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CytoSinct™ CD3 Nanobeads, Human

T cells were isolated from human PBMCs using CytoSinct™ Human CD3 Nanobeads and CytoSinctTM gM columns. CD3+ cells were stained with anti- CD3 (UCHT1) PE and gated on Live/hCD45+.

CytoSinct™ CD3 Nanobeads, human

The CytoSinct™ CD3 Nanobeads, human are used for separating CD3+ cells from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products or single cell suspension based on the surface expression of human CD3. The surface of Nanobeads are labeled with anti-human CD3 monoclonal antibody. To begin the isolation, Nanobeads are added to the cells. The CD3 antibodies preconjugated to the Nanobeads can bind the target cells expressing CD3 on cell surface. The cells/beads mixed suspension is loaded onto a CytoSinctTM Column which is effectively magnetized by an external magnetic field from CytoSinct™ Magnet, or other compatible cell isolation columns and magnets. The Nanobeads-labeled CD3+ cells are retained within the column and enriched during the wash step when Isolation Buffer is used to flush out the CD3- cells. After removing magnetic field, the target CD3+ cells can be easily eluted from the column.
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Description

The CytoSinct™ CD3 Nanobeads, human are used for separating CD3+ cells from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products or single cell suspension based on the surface expression of human CD3. The surface of Nanobeads are labeled with anti-human CD3 monoclonal antibody. To begin the isolation, Nanobeads are added to the cells. The CD3 antibodies preconjugated to the Nanobeads can bind the target cells expressing CD3 on cell surface. The cells/beads mixed suspension is loaded onto a CytoSinctTM Column which is effectively magnetized by an external magnetic field from CytoSinct™ Magnet, or other compatible cell isolation columns and magnets. The Nanobeads-labeled CD3+ cells are retained within the column and enriched during the wash step when Isolation Buffer is used to flush out the CD3- cells. After removing magnetic field, the target CD3+ cells can be easily eluted from the column.
Note

For research use only. Not intended for human and animal therapeutic or diagnostic use.

Properties
Reactivity Human
Formulation Bio-degradable matrix coated nanoparticle conjugated with anti-CD3 antibodies supplied in phosphate buffered-saline (PBS), containing Human Serum Albumin (HSA), pH 7.0-7.4.
Storage Store at 2-8 °C. Do not freeze.

Applications
Positive selection or depletion of CD3+ T cells from leukapheresis, PBMC or cell cultures. Isolated CD3+ T cells can be used for culture and expansion, flow cytometry, T cells functional assays.

Examples
  • CytoSinct™ CD3 Nanobeads, Human
    • CytoSinct™ CD3 Nanobeads, Human

    T cells were isolated from human PBMCs using CytoSinct™ Human CD3 Nanobeads and CytoSinctTM gM columns. CD3+ cells were stained with anti- CD3 (UCHT1) PE and gated on Live/hCD45+.