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High Affinity Ni-NTA Resin

*This product has been discontinued!*
Protein Purification
L00250
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Description

none
Applications
Applications:
1. Purification of polyhistidine-tagged proteins under native conditions.
2. Purification of polyhistidine-tagged proteins from E. coli under denaturing conditions.
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Overview
Key Features 产品特征
基质4%琼脂糖
平均粒径90 μm（45-165 μm）
吸附量≥20 mg 6xHis-tagged 蛋白（27 kDa）
储存溶液含20%乙醇的1×PBS
Ni-NTA亲和层析介质可耐受试剂
变性剂表面活性剂还原剂盐其他 
6 M 盐酸胍2%Triton X-100 20 mM β-ME4 M MgCl250%甘油
8 M尿素2% Tween 201 mM DTT5 mM CaCl220%乙醇
——1% CHAPS——2 M NaCl  1 mM EDTA
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Properties
Storage 2-8℃；DO NOT FREEZE
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相关文献
ZiyuanHaoXiWangYaxianZongShaoyingWenYanliChengHuogenLi, et al. Enzymatic activity and functional analysis under multiple abiotic stress conditions of a dehydroascorbate reductase gene derived from Liriodendron Chinense. Environmental and Experimental Botany. (2019)