gart cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gart
Entrez Gene ID	102236613
Full Name	phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
Gene Type	protein-coding
Organism	Xiphophorus maculatus(southern platyfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023329334.1	XP_023185102.1	trifunctional purine biosynthetic protein adenosine-3 isoform X2
XM_005813110.1	XP_005813167.1	trifunctional purine biosynthetic protein adenosine-3
XM_023329333.1	XP_023185101.1	trifunctional purine biosynthetic protein adenosine-3 isoform X1

Pathways from BioSystems
Gene Ontology

The following gart gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gart cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXi34883	XM_023329333.1 Latest version!	Xiphophorus maculatus phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (gart), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXi34884	XM_023329334.1 Latest version!	Xiphophorus maculatus phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (gart), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gart ( XM_023329333.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023185101.1 Xiphophorus maculatus phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (gart), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXi34883
Clone ID Related Accession (Same CDS sequence)	XM_023329333.1
Accession Version	XM_023329333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	trifunctional purine biosynthetic protein adenosine-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036466.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GGGTTCTGGT GGTAGGAGGC GGGGGACGGG AACACGCGCT GGCCTGGAAG 
CTGGCCCAGT CGCCCCGGGT CCAGCAGGTT CTGGTGGCTC CCGGAAACGC AGGAACCGCC 
AGCTGCGGCA AGATCAGCAA CTCTGAGGTG TCGGTGAGCA ACCACAGCAT CCTGGCTCAG 
TTCTGTAAGG ACCACCACGT CGGTTTGGTC GTAGTCGGAC CCGAGGCGCC GCTGGCAGCA 
GGTATTGTGG ACGATCTGAC GGCGGCGGGC ATTTTGTGTT TTGGACCGTC TGCTGCGGCG 
GCTCGGTTGG AGTCCAGTAA GAGTTTTGCA AAGGCGTTCA TGGAGCGTCA CGGCGTTCCC 
ACGGCTCGCT ACGGCTCCTT CAGCGACCCG CAGGACGCCT GCAGCTTCAT CCGCGGCGCC 
GACTTTCCAG CTCTGGTGGT GAAGGCCAGT GGGCTGGCGG CGGGGAAGGG AGTCATCGTC 
GCCGGCGATC GGGACGAAGC CTGCCGGGCC GTCATGGAGA TCATGAAGGA CGGAGCCTTT 
GGGTCAGCAG GAACAACGGT TGTTATTGAG GAGCTTTTGG AGGGACAGGA AGTGTCTTGT 
CTGTGCTTCA GCGACGGTAC CTGGGTCTCT CCGATGCCAC CGGCTCAGGA CCACAAGCGA 
CTCCAGGACG GAGACGCGGG GCCCCTCACG GGCGGGATGG GAGCCTACTG CCCCACCCCA 
CAGGTGAGCG AGGAGCTGCT GCAGCAGATC AGAGAGTCGA TCCTGCAGAA GACTGTGGAT 
GGGATGAGGC AGGAAGGAAC TCCTTATATG GGCGTTCTGT ACGCGGGCCT CATGCTGACC 
AATCGGGGGC CAAAAGTTCT GGAGTTCAAC TGTCGCTTTG GCGACCCAGA ATGCCAGGTT 
CTTCTTCCTC TGCTGCAGAG CGACCTCTAT GACGTCATCA TGAACACCGT AAGCGGGAAC 
CTGGCCTCTA ACGTCCCCGT GTGGCAGCAG GGCAGCTCTG CTGTTACCGT GGTGATGGCC 
AGCGCAGGAT ACCCGGGTTC CTACGTGAAA GGGGTCGAGA TCACAGGTCT GACCCGGCTG 
CAGGAGGCCG GGCTCCAGGC GTTCCACGCC GGCACCGCCC TGAAGGACGA CCGGGTGGTG 
TCGAGTGGCG GCAGAGTGCT GGCCGTGACG GCGGTGAGGT CGAGCCTGGA GGTCGCCCTG 
CAGGCGGCCA ATCAGGGCGT GGCTGCTGTT GGATTCCCGG GCGCCGTGTA CCGGCGAGAC 
ATCGGCCACC GGGCCATCGC TCACCAGAAC CAGAACCGCA GGCGTCTGAC CTACAAGGAC 
AGCGGAGTGG ACATCGCTGC TGGGAACCAG CTGGTGGACA TCATCAAGCC TCTGGCCAGA 
GCCACGACCC GACCCGGCTG CGATGCAGAG CTGGGAGGTT TTGCTGGACT GTTTGACCTG 
AAGGCAGCCG GGTTTGTTGA CCCGATCCTG GTTTCTGGGA CAGACGGTGT TGGAACTAAG 
CTGAAGATCG CCCAGGCCTG TGGCCGTCAT GGCGGTCTGG GTCAGGACTT GGTTGCCATG 
TGTGTGAACG ATGTTTTGGC TCACGGCGCC GAGCCGCTCT TCTTCCTGGA CTACTTCTCT 
TGCGGCAGTC TGGATGTAGA AGTTGCCGCC TCCATCGTCG GCGGCGTCGC CAAGGCGTGT 
GAGGCGGCCG GCTGCGCCCT GCTGGGCGGT GAAACGGCAG AGATGCCCGG TGTTTATGCT 
GCCGGAGAGT ACGACCTGGC CGGGTTCTGT GTCGGCGCGG CGGAGCGGGG CGCCTTGTTG 
CCACGGCTAC AGGACATCTC GGAGGGAGAC GTGCTGATCG GCGTGGCTTC CTCTGGGATT 
CACAGCAACG GCTTCAGTCT GGTCCGTAAA GTTCTGGAGA GAAGCAAACT GAGTTTGAGC 
TCTGCTGCTC CGTTTGGGAA CCAGGACCAG ACTGTCGGCG AGGTGTTGCT GACTCCTACT 
AAGATCTACA GCCGCCTGCT GCTGCCCGTC CTGCGCAGCG GGGCGGTTAA AGCCTACGCT 
CACATCACCG GTGGGGGGCT TCTGGAGAAC CTGCCCCGGG TTCTGCCCCA GGGTCTGGCA 
GCAGAGTTGG ATGCGTCCCA GTGGAGGATT CCAGCTGTTT TCTCTTGGCT CTATGAAGAA 
GGATCTCTGA GTGAGGAGGA GACGAGCAGA ACCTTCAACT GTGGGCTCGG TGCGGTTCTG 
GTGGTCGGTC CGGCCGATGC TCAGAAGGTT CTGTCTCAGC TGCAGGTCCA TGATGAAGCC 
TGGGTGGTGG GCTCTCTTGT TCCTAAGCAA CCAGATGTTT CTTCTGTGGT GGTTTTCAAC 
CTGAAGCAGA GCTTGATGAA TGCTGGGACT GTGGACGTAA AGGAACATGG TTTCCATGGA 
AATCAAATCA CAGCACCCAA GAAGAGCAGA GTAGCAGTTC TCATCTCTGG TACAGGTACC 
AATCTGCAGG CCCTGATTGA TCAGGCTAAA GCTCCAGGCA GCTCAGCAGA GATCGTGTTG 
GTGGTTTCAA ACAGACCTGG AGTTCAGGGT CTGAAGAGAG CAGCGCTGGC CGGCATCCAG 
ACCAGGGTTG TGGATCACAA GCTGTATGGC AGCAGAGCTG AGTTTGATGC CACCATTGAC 
CTTCTCCTTA AAGAGTTTGG AGTGGAACTG GTCTGCCTTG CTGGTTTCAT GAGGATCCTG 
ACTGGGATCT TTGTGAAAAA ATGGCATGGG AGGCTCCTCA ACATTCATCC CTCCCTGCTG 
CCTTCATTCA AAGGGGTCAA TGCCCAGCAA CAGGCTCTCC AGGCCAGAGT GCTGATAACT 
GGGTGCACCG TCCACTTTGT AGCTGAAGAG GTTGATGCAG GCGCCATCGT GGTCCAGGAA 
GCCGTTCCGG TGTTGGTTGG AGACACAGAG GAAAGTCTGT CGGCCAGAAT CAGGGAGGCC 
GAGCACAAAG CGTTCCCTAA AGCTCTGGAG CTGGTGGCCA GTGGAGCGGT GCAACTGGGA 
CCAGATGGAA ACATAGTCTG GAAGCAACAG CGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023185101.1
CDS	675..3710
Translation	MAERVLVVGGGGREHALAWKLAQSPRVQQVLVAPGNAGTASCGKISNSEVSVSNHSILAQFCKDHHVGLVVVGPEAPLAAGIVDDLTAAGILCFGPSAAAARLESSKSFAKAFMERHGVPTARYGSFSDPQDACSFIRGADFPALVVKASGLAAGKGVIVAGDRDEACRAVMEIMKDGAFGSAGTTVVIEELLEGQEVSCLCFSDGTWVSPMPPAQDHKRLQDGDAGPLTGGMGAYCPTPQVSEELLQQIRESILQKTVDGMRQEGTPYMGVLYAGLMLTNRGPKVLEFNCRFGDPECQVLLPLLQSDLYDVIMNTVSGNLASNVPVWQQGSSAVTVVMASAGYPGSYVKGVEITGLTRLQEAGLQAFHAGTALKDDRVVSSGGRVLAVTAVRSSLEVALQAANQGVAAVGFPGAVYRRDIGHRAIAHQNQNRRRLTYKDSGVDIAAGNQLVDIIKPLARATTRPGCDAELGGFAGLFDLKAAGFVDPILVSGTDGVGTKLKIAQACGRHGGLGQDLVAMCVNDVLAHGAEPLFFLDYFSCGSLDVEVAASIVGGVAKACEAAGCALLGGETAEMPGVYAAGEYDLAGFCVGAAERGALLPRLQDISEGDVLIGVASSGIHSNGFSLVRKVLERSKLSLSSAAPFGNQDQTVGEVLLTPTKIYSRLLLPVLRSGAVKAYAHITGGGLLENLPRVLPQGLAAELDASQWRIPAVFSWLYEEGSLSEEETSRTFNCGLGAVLVVGPADAQKVLSQLQVHDEAWVVGSLVPKQPDVSSVVVFNLKQSLMNAGTVDVKEHGFHGNQITAPKKSRVAVLISGTGTNLQALIDQAKAPGSSAEIVLVVSNRPGVQGLKRAALAGIQTRVVDHKLYGSRAEFDATIDLLLKEFGVELVCLAGFMRILTGIFVKKWHGRLLNIHPSLLPSFKGVNAQQQALQARVLITGCTVHFVAEEVDAGAIVVQEAVPVLVGDTEESLSARIREAEHKAFPKALELVASGAVQLGPDGNIVWKQQR

Target ORF information:

RefSeq Version	XM_023329333.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (gart), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023329333.1

ORF Insert Sequence:

ATGGCGGAGC GGGTTCTGGT GGTAGGAGGC GGGGGACGGG AACACGCGCT GGCCTGGAAG 
CTGGCCCAGT CGCCCCGGGT CCAGCAGGTT CTGGTGGCTC CCGGAAACGC AGGAACCGCC 
AGCTGCGGCA AGATCAGCAA CTCTGAGGTG TCGGTGAGCA ACCACAGCAT CCTGGCTCAG 
TTCTGTAAGG ACCACCACGT CGGTTTGGTC GTAGTCGGAC CCGAGGCGCC GCTGGCAGCA 
GGTATTGTGG ACGATCTGAC GGCGGCGGGC ATTTTGTGTT TTGGACCGTC TGCTGCGGCG 
GCTCGGTTGG AGTCCAGTAA GAGTTTTGCA AAGGCGTTCA TGGAGCGTCA CGGCGTTCCC 
ACGGCTCGCT ACGGCTCCTT CAGCGACCCG CAGGACGCCT GCAGCTTCAT CCGCGGCGCC 
GACTTTCCAG CTCTGGTGGT GAAGGCCAGT GGGCTGGCGG CGGGGAAGGG AGTCATCGTC 
GCCGGCGATC GGGACGAAGC CTGCCGGGCC GTCATGGAGA TCATGAAGGA CGGAGCCTTT 
GGGTCAGCAG GAACAACGGT TGTTATTGAG GAGCTTTTGG AGGGACAGGA AGTGTCTTGT 
CTGTGCTTCA GCGACGGTAC CTGGGTCTCT CCGATGCCAC CGGCTCAGGA CCACAAGCGA 
CTCCAGGACG GAGACGCGGG GCCCCTCACG GGCGGGATGG GAGCCTACTG CCCCACCCCA 
CAGGTGAGCG AGGAGCTGCT GCAGCAGATC AGAGAGTCGA TCCTGCAGAA GACTGTGGAT 
GGGATGAGGC AGGAAGGAAC TCCTTATATG GGCGTTCTGT ACGCGGGCCT CATGCTGACC 
AATCGGGGGC CAAAAGTTCT GGAGTTCAAC TGTCGCTTTG GCGACCCAGA ATGCCAGGTT 
CTTCTTCCTC TGCTGCAGAG CGACCTCTAT GACGTCATCA TGAACACCGT AAGCGGGAAC 
CTGGCCTCTA ACGTCCCCGT GTGGCAGCAG GGCAGCTCTG CTGTTACCGT GGTGATGGCC 
AGCGCAGGAT ACCCGGGTTC CTACGTGAAA GGGGTCGAGA TCACAGGTCT GACCCGGCTG 
CAGGAGGCCG GGCTCCAGGC GTTCCACGCC GGCACCGCCC TGAAGGACGA CCGGGTGGTG 
TCGAGTGGCG GCAGAGTGCT GGCCGTGACG GCGGTGAGGT CGAGCCTGGA GGTCGCCCTG 
CAGGCGGCCA ATCAGGGCGT GGCTGCTGTT GGATTCCCGG GCGCCGTGTA CCGGCGAGAC 
ATCGGCCACC GGGCCATCGC TCACCAGAAC CAGAACCGCA GGCGTCTGAC CTACAAGGAC 
AGCGGAGTGG ACATCGCTGC TGGGAACCAG CTGGTGGACA TCATCAAGCC TCTGGCCAGA 
GCCACGACCC GACCCGGCTG CGATGCAGAG CTGGGAGGTT TTGCTGGACT GTTTGACCTG 
AAGGCAGCCG GGTTTGTTGA CCCGATCCTG GTTTCTGGGA CAGACGGTGT TGGAACTAAG 
CTGAAGATCG CCCAGGCCTG TGGCCGTCAT GGCGGTCTGG GTCAGGACTT GGTTGCCATG 
TGTGTGAACG ATGTTTTGGC TCACGGCGCC GAGCCGCTCT TCTTCCTGGA CTACTTCTCT 
TGCGGCAGTC TGGATGTAGA AGTTGCCGCC TCCATCGTCG GCGGCGTCGC CAAGGCGTGT 
GAGGCGGCCG GCTGCGCCCT GCTGGGCGGT GAAACGGCAG AGATGCCCGG TGTTTATGCT 
GCCGGAGAGT ACGACCTGGC CGGGTTCTGT GTCGGCGCGG CGGAGCGGGG CGCCTTGTTG 
CCACGGCTAC AGGACATCTC GGAGGGAGAC GTGCTGATCG GCGTGGCTTC CTCTGGGATT 
CACAGCAACG GCTTCAGTCT GGTCCGTAAA GTTCTGGAGA GAAGCAAACT GAGTTTGAGC 
TCTGCTGCTC CGTTTGGGAA CCAGGACCAG ACTGTCGGCG AGGTGTTGCT GACTCCTACT 
AAGATCTACA GCCGCCTGCT GCTGCCCGTC CTGCGCAGCG GGGCGGTTAA AGCCTACGCT 
CACATCACCG GTGGGGGGCT TCTGGAGAAC CTGCCCCGGG TTCTGCCCCA GGGTCTGGCA 
GCAGAGTTGG ATGCGTCCCA GTGGAGGATT CCAGCTGTTT TCTCTTGGCT CTATGAAGAA 
GGATCTCTGA GTGAGGAGGA GACGAGCAGA ACCTTCAACT GTGGGCTCGG TGCGGTTCTG 
GTGGTCGGTC CGGCCGATGC TCAGAAGGTT CTGTCTCAGC TGCAGGTCCA TGATGAAGCC 
TGGGTGGTGG GCTCTCTTGT TCCTAAGCAA CCAGATGTTT CTTCTGTGGT GGTTTTCAAC 
CTGAAGCAGA GCTTGATGAA TGCTGGGACT GTGGACGTAA AGGAACATGG TTTCCATGGA 
AATCAAATCA CAGCACCCAA GAAGAGCAGA GTAGCAGTTC TCATCTCTGG TACAGGTACC 
AATCTGCAGG CCCTGATTGA TCAGGCTAAA GCTCCAGGCA GCTCAGCAGA GATCGTGTTG 
GTGGTTTCAA ACAGACCTGG AGTTCAGGGT CTGAAGAGAG CAGCGCTGGC CGGCATCCAG 
ACCAGGGTTG TGGATCACAA GCTGTATGGC AGCAGAGCTG AGTTTGATGC CACCATTGAC 
CTTCTCCTTA AAGAGTTTGG AGTGGAACTG GTCTGCCTTG CTGGTTTCAT GAGGATCCTG 
ACTGGGATCT TTGTGAAAAA ATGGCATGGG AGGCTCCTCA ACATTCATCC CTCCCTGCTG 
CCTTCATTCA AAGGGGTCAA TGCCCAGCAA CAGGCTCTCC AGGCCAGAGT GCTGATAACT 
GGGTGCACCG TCCACTTTGT AGCTGAAGAG GTTGATGCAG GCGCCATCGT GGTCCAGGAA 
GCCGTTCCGG TGTTGGTTGG AGACACAGAG GAAAGTCTGT CGGCCAGAAT CAGGGAGGCC 
GAGCACAAAG CGTTCCCTAA AGCTCTGGAG CTGGTGGCCA GTGGAGCGGT GCAACTGGGA 
CCAGATGGAA ACATAGTCTG GAAGCAACAG CGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gart ( XM_023329334.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023185102.1 Xiphophorus maculatus phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (gart), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXi34884
Clone ID Related Accession (Same CDS sequence)	XM_023329334.1
Accession Version	XM_023329334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	trifunctional purine biosynthetic protein adenosine-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036466.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GGGTTCTGGT GGTAGGAGGC GGGGGACGGG AACACGCGCT GGCCTGGAAG 
CTGGCCCAGT CGCCCCGGGT CCAGCAGGTT CTGGTGGCTC CCGGAAACGC AGGAACCGCC 
AGCTGCGGCA AGATCAGCAA CTCTGAGGTG TCGGTGAGCA ACCACAGCAT CCTGGCTCAG 
TTCTGTAAGG ACCACCACGT CGGTTTGGTC GTAGTCGGAC CCGAGGCGCC GCTGGCAGCA 
GGTATTGTGG ACGATCTGAC GGCGGCGGGC ATTTTGTGTT TTGGACCGTC TGCTGCGGCG 
GCTCGGTTGG AGTCCAGTAA GAGTTTTGCA AAGGCGTTCA TGGAGCGTCA CGGCGTTCCC 
ACGGCTCGCT ACGGCTCCTT CAGCGACCCG CAGGACGCCT GCAGCTTCAT CCGCGGCGCC 
GACTTTCCAG CTCTGGTGGT GAAGGCCAGT GGGCTGGCGG CGGGGAAGGG AGTCATCGTC 
GCCGGCGATC GGGACGAAGC CTGCCGGGCC GTCATGGAGA TCATGAAGGA CGGAGCCTTT 
GGGTCAGCAG GAACAACGGT TGTTATTGAG GAGCTTTTGG AGGGACAGGA AGTGTCTTGT 
CTGTGCTTCA GCGACGGTAC CTGGGTCTCT CCGATGCCAC CGGCTCAGGA CCACAAGCGA 
CTCCAGGACG GAGACGCGGG GCCCCTCACG GGCGGGATGG GAGCCTACTG CCCCACCCCA 
CAGGTGAGCG AGGAGCTGCT GCAGCAGATC AGAGAGTCGA TCCTGCAGAA GACTGTGGAT 
GGGATGAGGC AGGAAGGAAC TCCTTATATG GGCGTTCTGT ACGCGGGCCT CATGCTGACC 
AATCGGGGGC CAAAAGTTCT GGAGTTCAAC TGTCGCTTTG GCGACCCAGA ATGCCAGGTT 
CTTCTTCCTC TGCTGCAGAG CGACCTCTAT GACGTCATCA TGAACACCGT AAGCGGGAAC 
CTGGCCTCTA ACGTCCCCGT GTGGCAGCAG GGCAGCTCTG CTGTTACCGT GGTGATGGCC 
AGCGCAGGAT ACCCGGGTTC CTACGTGAAA GGGGTCGAGA TCACAGGTCT GACCCGGCTG 
CAGGAGGCCG GGCTCCAGGC GTTCCACGCC GGCACCGCCC TGAAGGACGA CCGGGTGGTG 
TCGAGTGGCG GCAGAGTGCT GGCCGTGACG GCGGTGAGGT CGAGCCTGGA GGTCGCCCTG 
CAGGCGGCCA ATCAGGGCGT GGCTGCTGTT GGATTCCCGG GCGCCGTGTA CCGGCGAGAC 
ATCGGCCACC GGGCCATCGC TCACCAGAAC CAGAACCGGC GTCTGACCTA CAAGGACAGC 
GGAGTGGACA TCGCTGCTGG GAACCAGCTG GTGGACATCA TCAAGCCTCT GGCCAGAGCC 
ACGACCCGAC CCGGCTGCGA TGCAGAGCTG GGAGGTTTTG CTGGACTGTT TGACCTGAAG 
GCAGCCGGGT TTGTTGACCC GATCCTGGTT TCTGGGACAG ACGGTGTTGG AACTAAGCTG 
AAGATCGCCC AGGCCTGTGG CCGTCATGGC GGTCTGGGTC AGGACTTGGT TGCCATGTGT 
GTGAACGATG TTTTGGCTCA CGGCGCCGAG CCGCTCTTCT TCCTGGACTA CTTCTCTTGC 
GGCAGTCTGG ATGTAGAAGT TGCCGCCTCC ATCGTCGGCG GCGTCGCCAA GGCGTGTGAG 
GCGGCCGGCT GCGCCCTGCT GGGCGGTGAA ACGGCAGAGA TGCCCGGTGT TTATGCTGCC 
GGAGAGTACG ACCTGGCCGG GTTCTGTGTC GGCGCGGCGG AGCGGGGCGC CTTGTTGCCA 
CGGCTACAGG ACATCTCGGA GGGAGACGTG CTGATCGGCG TGGCTTCCTC TGGGATTCAC 
AGCAACGGCT TCAGTCTGGT CCGTAAAGTT CTGGAGAGAA GCAAACTGAG TTTGAGCTCT 
GCTGCTCCGT TTGGGAACCA GGACCAGACT GTCGGCGAGG TGTTGCTGAC TCCTACTAAG 
ATCTACAGCC GCCTGCTGCT GCCCGTCCTG CGCAGCGGGG CGGTTAAAGC CTACGCTCAC 
ATCACCGGTG GGGGGCTTCT GGAGAACCTG CCCCGGGTTC TGCCCCAGGG TCTGGCAGCA 
GAGTTGGATG CGTCCCAGTG GAGGATTCCA GCTGTTTTCT CTTGGCTCTA TGAAGAAGGA 
TCTCTGAGTG AGGAGGAGAC GAGCAGAACC TTCAACTGTG GGCTCGGTGC GGTTCTGGTG 
GTCGGTCCGG CCGATGCTCA GAAGGTTCTG TCTCAGCTGC AGGTCCATGA TGAAGCCTGG 
GTGGTGGGCT CTCTTGTTCC TAAGCAACCA GATGTTTCTT CTGTGGTGGT TTTCAACCTG 
AAGCAGAGCT TGATGAATGC TGGGACTGTG GACGTAAAGG AACATGGTTT CCATGGAAAT 
CAAATCACAG CACCCAAGAA GAGCAGAGTA GCAGTTCTCA TCTCTGGTAC AGGTACCAAT 
CTGCAGGCCC TGATTGATCA GGCTAAAGCT CCAGGCAGCT CAGCAGAGAT CGTGTTGGTG 
GTTTCAAACA GACCTGGAGT TCAGGGTCTG AAGAGAGCAG CGCTGGCCGG CATCCAGACC 
AGGGTTGTGG ATCACAAGCT GTATGGCAGC AGAGCTGAGT TTGATGCCAC CATTGACCTT 
CTCCTTAAAG AGTTTGGAGT GGAACTGGTC TGCCTTGCTG GTTTCATGAG GATCCTGACT 
GGGATCTTTG TGAAAAAATG GCATGGGAGG CTCCTCAACA TTCATCCCTC CCTGCTGCCT 
TCATTCAAAG GGGTCAATGC CCAGCAACAG GCTCTCCAGG CCAGAGTGCT GATAACTGGG 
TGCACCGTCC ACTTTGTAGC TGAAGAGGTT GATGCAGGCG CCATCGTGGT CCAGGAAGCC 
GTTCCGGTGT TGGTTGGAGA CACAGAGGAA AGTCTGTCGG CCAGAATCAG GGAGGCCGAG 
CACAAAGCGT TCCCTAAAGC TCTGGAGCTG GTGGCCAGTG GAGCGGTGCA ACTGGGACCA 
GATGGAAACA TAGTCTGGAA GCAACAGCGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023185102.1
CDS	675..3707
Translation	MAERVLVVGGGGREHALAWKLAQSPRVQQVLVAPGNAGTASCGKISNSEVSVSNHSILAQFCKDHHVGLVVVGPEAPLAAGIVDDLTAAGILCFGPSAAAARLESSKSFAKAFMERHGVPTARYGSFSDPQDACSFIRGADFPALVVKASGLAAGKGVIVAGDRDEACRAVMEIMKDGAFGSAGTTVVIEELLEGQEVSCLCFSDGTWVSPMPPAQDHKRLQDGDAGPLTGGMGAYCPTPQVSEELLQQIRESILQKTVDGMRQEGTPYMGVLYAGLMLTNRGPKVLEFNCRFGDPECQVLLPLLQSDLYDVIMNTVSGNLASNVPVWQQGSSAVTVVMASAGYPGSYVKGVEITGLTRLQEAGLQAFHAGTALKDDRVVSSGGRVLAVTAVRSSLEVALQAANQGVAAVGFPGAVYRRDIGHRAIAHQNQNRRLTYKDSGVDIAAGNQLVDIIKPLARATTRPGCDAELGGFAGLFDLKAAGFVDPILVSGTDGVGTKLKIAQACGRHGGLGQDLVAMCVNDVLAHGAEPLFFLDYFSCGSLDVEVAASIVGGVAKACEAAGCALLGGETAEMPGVYAAGEYDLAGFCVGAAERGALLPRLQDISEGDVLIGVASSGIHSNGFSLVRKVLERSKLSLSSAAPFGNQDQTVGEVLLTPTKIYSRLLLPVLRSGAVKAYAHITGGGLLENLPRVLPQGLAAELDASQWRIPAVFSWLYEEGSLSEEETSRTFNCGLGAVLVVGPADAQKVLSQLQVHDEAWVVGSLVPKQPDVSSVVVFNLKQSLMNAGTVDVKEHGFHGNQITAPKKSRVAVLISGTGTNLQALIDQAKAPGSSAEIVLVVSNRPGVQGLKRAALAGIQTRVVDHKLYGSRAEFDATIDLLLKEFGVELVCLAGFMRILTGIFVKKWHGRLLNIHPSLLPSFKGVNAQQQALQARVLITGCTVHFVAEEVDAGAIVVQEAVPVLVGDTEESLSARIREAEHKAFPKALELVASGAVQLGPDGNIVWKQQR

Target ORF information:

RefSeq Version	XM_023329334.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (gart), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023329334.1

ORF Insert Sequence:

ATGGCGGAGC GGGTTCTGGT GGTAGGAGGC GGGGGACGGG AACACGCGCT GGCCTGGAAG 
CTGGCCCAGT CGCCCCGGGT CCAGCAGGTT CTGGTGGCTC CCGGAAACGC AGGAACCGCC 
AGCTGCGGCA AGATCAGCAA CTCTGAGGTG TCGGTGAGCA ACCACAGCAT CCTGGCTCAG 
TTCTGTAAGG ACCACCACGT CGGTTTGGTC GTAGTCGGAC CCGAGGCGCC GCTGGCAGCA 
GGTATTGTGG ACGATCTGAC GGCGGCGGGC ATTTTGTGTT TTGGACCGTC TGCTGCGGCG 
GCTCGGTTGG AGTCCAGTAA GAGTTTTGCA AAGGCGTTCA TGGAGCGTCA CGGCGTTCCC 
ACGGCTCGCT ACGGCTCCTT CAGCGACCCG CAGGACGCCT GCAGCTTCAT CCGCGGCGCC 
GACTTTCCAG CTCTGGTGGT GAAGGCCAGT GGGCTGGCGG CGGGGAAGGG AGTCATCGTC 
GCCGGCGATC GGGACGAAGC CTGCCGGGCC GTCATGGAGA TCATGAAGGA CGGAGCCTTT 
GGGTCAGCAG GAACAACGGT TGTTATTGAG GAGCTTTTGG AGGGACAGGA AGTGTCTTGT 
CTGTGCTTCA GCGACGGTAC CTGGGTCTCT CCGATGCCAC CGGCTCAGGA CCACAAGCGA 
CTCCAGGACG GAGACGCGGG GCCCCTCACG GGCGGGATGG GAGCCTACTG CCCCACCCCA 
CAGGTGAGCG AGGAGCTGCT GCAGCAGATC AGAGAGTCGA TCCTGCAGAA GACTGTGGAT 
GGGATGAGGC AGGAAGGAAC TCCTTATATG GGCGTTCTGT ACGCGGGCCT CATGCTGACC 
AATCGGGGGC CAAAAGTTCT GGAGTTCAAC TGTCGCTTTG GCGACCCAGA ATGCCAGGTT 
CTTCTTCCTC TGCTGCAGAG CGACCTCTAT GACGTCATCA TGAACACCGT AAGCGGGAAC 
CTGGCCTCTA ACGTCCCCGT GTGGCAGCAG GGCAGCTCTG CTGTTACCGT GGTGATGGCC 
AGCGCAGGAT ACCCGGGTTC CTACGTGAAA GGGGTCGAGA TCACAGGTCT GACCCGGCTG 
CAGGAGGCCG GGCTCCAGGC GTTCCACGCC GGCACCGCCC TGAAGGACGA CCGGGTGGTG 
TCGAGTGGCG GCAGAGTGCT GGCCGTGACG GCGGTGAGGT CGAGCCTGGA GGTCGCCCTG 
CAGGCGGCCA ATCAGGGCGT GGCTGCTGTT GGATTCCCGG GCGCCGTGTA CCGGCGAGAC 
ATCGGCCACC GGGCCATCGC TCACCAGAAC CAGAACCGGC GTCTGACCTA CAAGGACAGC 
GGAGTGGACA TCGCTGCTGG GAACCAGCTG GTGGACATCA TCAAGCCTCT GGCCAGAGCC 
ACGACCCGAC CCGGCTGCGA TGCAGAGCTG GGAGGTTTTG CTGGACTGTT TGACCTGAAG 
GCAGCCGGGT TTGTTGACCC GATCCTGGTT TCTGGGACAG ACGGTGTTGG AACTAAGCTG 
AAGATCGCCC AGGCCTGTGG CCGTCATGGC GGTCTGGGTC AGGACTTGGT TGCCATGTGT 
GTGAACGATG TTTTGGCTCA CGGCGCCGAG CCGCTCTTCT TCCTGGACTA CTTCTCTTGC 
GGCAGTCTGG ATGTAGAAGT TGCCGCCTCC ATCGTCGGCG GCGTCGCCAA GGCGTGTGAG 
GCGGCCGGCT GCGCCCTGCT GGGCGGTGAA ACGGCAGAGA TGCCCGGTGT TTATGCTGCC 
GGAGAGTACG ACCTGGCCGG GTTCTGTGTC GGCGCGGCGG AGCGGGGCGC CTTGTTGCCA 
CGGCTACAGG ACATCTCGGA GGGAGACGTG CTGATCGGCG TGGCTTCCTC TGGGATTCAC 
AGCAACGGCT TCAGTCTGGT CCGTAAAGTT CTGGAGAGAA GCAAACTGAG TTTGAGCTCT 
GCTGCTCCGT TTGGGAACCA GGACCAGACT GTCGGCGAGG TGTTGCTGAC TCCTACTAAG 
ATCTACAGCC GCCTGCTGCT GCCCGTCCTG CGCAGCGGGG CGGTTAAAGC CTACGCTCAC 
ATCACCGGTG GGGGGCTTCT GGAGAACCTG CCCCGGGTTC TGCCCCAGGG TCTGGCAGCA 
GAGTTGGATG CGTCCCAGTG GAGGATTCCA GCTGTTTTCT CTTGGCTCTA TGAAGAAGGA 
TCTCTGAGTG AGGAGGAGAC GAGCAGAACC TTCAACTGTG GGCTCGGTGC GGTTCTGGTG 
GTCGGTCCGG CCGATGCTCA GAAGGTTCTG TCTCAGCTGC AGGTCCATGA TGAAGCCTGG 
GTGGTGGGCT CTCTTGTTCC TAAGCAACCA GATGTTTCTT CTGTGGTGGT TTTCAACCTG 
AAGCAGAGCT TGATGAATGC TGGGACTGTG GACGTAAAGG AACATGGTTT CCATGGAAAT 
CAAATCACAG CACCCAAGAA GAGCAGAGTA GCAGTTCTCA TCTCTGGTAC AGGTACCAAT 
CTGCAGGCCC TGATTGATCA GGCTAAAGCT CCAGGCAGCT CAGCAGAGAT CGTGTTGGTG 
GTTTCAAACA GACCTGGAGT TCAGGGTCTG AAGAGAGCAG CGCTGGCCGG CATCCAGACC 
AGGGTTGTGG ATCACAAGCT GTATGGCAGC AGAGCTGAGT TTGATGCCAC CATTGACCTT 
CTCCTTAAAG AGTTTGGAGT GGAACTGGTC TGCCTTGCTG GTTTCATGAG GATCCTGACT 
GGGATCTTTG TGAAAAAATG GCATGGGAGG CTCCTCAACA TTCATCCCTC CCTGCTGCCT 
TCATTCAAAG GGGTCAATGC CCAGCAACAG GCTCTCCAGG CCAGAGTGCT GATAACTGGG 
TGCACCGTCC ACTTTGTAGC TGAAGAGGTT GATGCAGGCG CCATCGTGGT CCAGGAAGCC 
GTTCCGGTGT TGGTTGGAGA CACAGAGGAA AGTCTGTCGG CCAGAATCAG GGAGGCCGAG 
CACAAAGCGT TCCCTAAAGC TCTGGAGCTG GTGGCCAGTG GAGCGGTGCA ACTGGGACCA 
GATGGAAACA TAGTCTGGAA GCAACAGCGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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gart cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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