cfap65 cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cfap65
Entrez Gene ID	102232253
Full Name	cilia and flagella associated protein 65
Gene Type	protein-coding
Organism	Xiphophorus maculatus(southern platyfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014475483.1	XP_014330969.1	coiled-coil domain-containing protein 108
XM_023336504.1	XP_023192272.1	cilia- and flagella-associated protein 65 isoform X1
XM_023336505.1	XP_023192273.1	cilia- and flagella-associated protein 65 isoform X2
XM_023336506.1	XP_023192274.1	cilia- and flagella-associated protein 65 isoform X3

Pathways from BioSystems
Gene Ontology

The following cfap65 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cfap65 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXi40281	XM_023336504.1 Latest version!	Xiphophorus maculatus cilia and flagella associated protein 65 (cfap65), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXi40282	XM_023336505.1 Latest version!	Xiphophorus maculatus cilia and flagella associated protein 65 (cfap65), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXi40283	XM_023336506.1 Latest version!	Xiphophorus maculatus cilia and flagella associated protein 65 (cfap65), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cfap65 ( XM_023336504.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023192272.1 Xiphophorus maculatus cilia and flagella associated protein 65 (cfap65), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXi40281
Clone ID Related Accession (Same CDS sequence)	XM_023336504.1
Accession Version	XM_023336504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	cilia- and flagella-associated protein 65 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036449.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGCTG AATCCCGCTG TCCTGCCTGT TTGGCGTCTG CTTTTTGTCG ATGGAAGCGC 
TCCAGCAGAG AGACTGACGT TAAAGAACCC CAACTCCCTG ATATAAAACA ATGTCAAAGA 
AAATCCCATC CCCAAAGAAG CATCTTCTTA GGCCTAGAAA CCACATTAGA TCTGACATGG 
AAGGACTGGG TCATTGAACG AGAGTACACC AAGACACTTG TGATAAGCAA CATCCGTAAT 
AAGCTGCAGA AGCTGCACAT TAGTGCTCCA GAGACCAAGT TTTTCTCAAT TTTGAATCCT 
GAAGTAGTTA TTCTCAGCCC AGGGACTTCA TTCTCCATAC CGGTCACTTT TAAACCTCTC 
CAAAAGTGTG GGTATAAGGA CTGCATTCAG TTTCAAGCTA TTGAAGGAAG CTTTAAAGTA 
TACCTGCATG CTGTGCTTTC AAGTCATACA ATGAAGGTGC CTGAATCTGT GATGCTGCCC 
CTATGTGCTG TTGAGCACTA CACACTAACC AGTTTCCCAC TAAAAAATAT CAGTAAGGTT 
CAGATGTCAT TTAAATGGAA TAGTGCAGCT CCTTTCCAAC TCAGTCCAGA ACAAGGCCGG 
CTAAAGCCCG GTCAGCAGTG TGTCATCACG GTGGACTTTC AGCCACTAGA GGCTCAGGTG 
TACCAGAAGC AGGTGCACTG CACATATGGA GAAGATGGCA ACAAAGCTAA TAGCTGCTGC 
ACTGTGCTTT TACAAGGGAT AGGAAAATAT CCATTTCTTC AATTAAGAAT TTCCAATGAC 
AAAGAGGAAA CCAGCCATGG TGATATTGAG TTGGACTTTG GGTCTGTGGC TGTTGGTCAC 
AGTTTGAGTA AACAATTTGA AATCTTCAAT CCTTCTCCTG TTAGTGTGTG GTTTTCTCTC 
TCACAACTGC CTGGAGCGGT AGCCATCTTT GGACCACACT TCAGTTGGGA TGTCACAAGT 
GAGAAAATAG TGCCTGGTGG TTCGGTCCAG GTTTTGGTTA CTTATTCTCC AACTATGGTC 
GACATAGTCT CTGTGGAGTA CTTGTCTTTG AATTATAGAG GGTCACTCAG TAAAAGTCAG 
GTCAAACTCA TAGGAAAGTG TATGGGTCCC GAACTCTGTT TGTCCTCCAC TGTGGTCGAC 
TTTGGGTGTG TTGAAGAAAA AGGATCAGCA GAACAAACAG TGGAACTGCT GAACTCTTCC 
CCTGTTCAGG CTACCTACCA ATGGGACATA GACTGTGCAA ACAGTGTTTT TAGAGTTTCA 
CCAGCAAGTG GCACTGTGCT TCCATATGGT CAAATCAAAG TCAAGGTCTC CTACAAACCC 
ACACACCCTA TGCCACACCA CAGAAGAGTG GCATGTCTTA TACTCCTCGG GGAGCCTCTG 
TTTCTGGACT TGATTGGTAC ATGCCACACA AATCTTCAGG AGCCAGTAGC ATTAACATTT 
GAGCACTTGG TTGAGTACAA GTCTAAGTTG TGCTATATGT GTAACTCATC AGACACACTG 
CTGGGGGTGC CTTTAGAAGA GGAAGGAAAT AGTGCTACTG CCATTCCTCC AGCACCTATG 
GAGAAATTGA ATGAAATCAG GGAAGAACAC GTGGATTCTC TCTTCTCACC ATCATTTCCA 
TTTGTATCTG TTGAACCAAG TGAACTGCTG TTTAATCACA AAACAAGATC CTTTATCGCT 
CCATCATTTG CTGTCTCACA GTGTGTTACC TTTAAAAATC ACACCAAAGA GAAACTCAGC 
CTCATGTGGA CCGTTATCCA AGATTCCCCT TTCTGTGTTA CTCCTTCGTC ATTTGAGTTG 
GCCCCACTGA AATCTACCAC TGCGCTTGTG ACATATGAAC CAAGACAGAT CAATGCCCTG 
CATGGAGGAG AGCTTGAGTG CTTTGCATAC TGTAAGATGC CACAGGAGGA TCCTTCCTCA 
GAACAAGACC TTCCATGTCT GCCTTGTTGT GTGACTGTCA GAGTCATTAG CCACTCTTTT 
CAGCCAGGGA AAGATCACTT CACTCCTTCC TGTATTTTGA AGCCCCACCA AGTGGTTTTT 
CCACCTCTGA GTCTTATTTC CTATCGGACC GTGCTACTGC AGAACAATGG CGATCAGCCT 
CTCACCTTCT GCCTGAACTA CAGAACAAAC AGTGACTTGC CAAGGTCTGT GCATATGTCA 
CCAGGCTGTG GTTTCATCCA GCCAGGACAA CACCAAATCC TTACCATTGG AGCTGTCCCC 
ACTCTGGATA GTCCCACAGA GGGGTTTAAT TTACCATTGC AGCTTAATGC AGATGAATTC 
ACGAAGGAAC TGACAGTTGT GAGCTCCTTA AAAAAGCCAT GTGTGTCTTT TGAAAATAGC 
AACGAATTAC ATTTCACTGC TACAGCTGTC GGCTCAAAAT CACAGCACAG CCATTCCATC 
AAGAATTTAA GCAGCGTGCC CATCACGTTT CAGTGGATCA TTTCAGAGAG AGATCAGAAT 
CTTATCTGCG TTGAACCAGA TGCTGGTAAA CTGCATCCCA ATGAGAGCTT GGTCCAGACA 
TGGACATTTT CTCCTCTGAC AGAGAAAGCA TACACATTCA AACCCACTCT CAGCTTCCAG 
AGCGATGACT TTAACAAGTC GCAACTCATT TTCAGAGTGA ATGGAACTGG TGCAGCTGGC 
ACCATTGAGG CAGGGAAAGA ACTCCTAGAT ATGGGAGAGA GCTTAGTTGG AAGCTGCCGG 
TCGTTTAATA TTCCTATAGT GAACAACTGT AGCTGCCCCA TCTCTTTTCA TCTGTCTGTA 
CAGCTAGAAG AAAATCCCCC TGATTCTGAA GATAACAGTG CTCTGCATCT GGACGTTGAA 
ACGGGAACAA TTGCTTCACA TTCCACTGTG CTAATCGGTT CCACTCTTAG ATTGGATGCA 
CAAGGCTGGT ACCGCTGGGC CATCAGCTAC CAGATTACAG ATGGCAATGG CTTTGGTGTA 
TGTCCTCCTG AGAAACTGTG TGAAGTGCGA GCTAAGGGGG TGTTCCCCAC CTTACAGGTG 
GTTGATGCAT GTAGCAGTGG CAGCGTGGAC AGGCTCTGCA AGCAGTATTT GTGGAAACTC 
TTCTCATTAG ACAGTTTTAA TGAGCACCTG CTTTCCACCC CATGTCCTGC AGAAATTACT 
TTCAAAGCGC CCACCAAGCA CAGACAGAAC AGTGTCTCCA GCTTCCCTCA AGTCATATTG 
GATTTCAATT TTGGTGCTGG TCCTCTGCAT TCAGATCCCT CAGATTTTAT GTTGATGTTT 
TGGAACCCCG GTTCTATCTC TGTAAACTGG ACATTCTTAT TTCCAGAGGA CCAAGAAATG 
AAGCTGGAGG ACTGGGCTAT GACAGGACAG TTTAGCAACA AAGAACTTCA GGAAATGAAG 
GTACAAGACA ATCAACTCTT CAGAGTTACC CCTCGTTCTG GTACTTTGCT TCCAGGCCAG 
AAATGTCCAG TACACTTCTG CTACAGTCAT GACTTTATTG GAATTGACCG ACTTCCTGTT 
TTGTTCAAAC TCAGTCTTGG CAGAGTTTTT TTGCTCAACT TTCAAGGAGT GACATTGAAC 
CGAAATGAAC CATATCTACA CTTTGCTTCC AATCACCATG TATTCAAGCC TGTAGCTATT 
CGGGACTCCA GTCCTCCACG ACAGATATAT GAGTTGTACA ACGGTGGTGC GGTGACAGTA 
CATTATGAAG TGGACCAAGT TGTATTGTCA CAGCTTCAGA TGGAGAACTA TGATCACCCA 
GTCTTGAGCT GTCTCAGTCC ACAAGGAGAA GTTCTCCCTG GGAATAAAGC CATGCTCGAA 
TGGATCTTCT CTCCATTGGA GGCTAAGATG TACCATGTGG ATGTTCCCAT CCACATCCGG 
GAAAAGGATT CGGTAATTAT AAACTTTGAG GGACGTGGGA TGAATGCATT ATCAACAAGC 
GCCAATATCT CCATTGAATA CAGCGATGCT AAGACTCAAG AACACCGTGC CCAGAAGGGG 
CCATTCCCAG GACAGGTGGC TTGGTTGTCC AAGGATAGTG TCTTTATTGG CGACATCCCT 
GTTTGCACAA AGTCTTCGAG AATCATGTTC ATCACCAGTT TGTCTTGCAC AGATACTGTG 
TACTTTGTCT GGGAGATTCC TACACAGAGC AACCAACAAG TGGTACAGAT CCATCCAGAA 
CGAGGCTGTC TTGGTCCAGG AGAGTGTGCT ATGTGCATCC TGACCTTCGT TTCTGATTTC 
CCCACTGTTT ATCAGCTAGA TCTCATATGT CAGGTCACTC AGAACGCTGC ACTCATCCAT 
TATCACAAAG CCTTGCAATG CTGGGAGGAA GAAAAGAAAC GACAGCAAAA CGAATTCACA 
ATCACAGACA AGATCCCTTC CCGCACTCAA AGAGTTCTAG TAGATGAAAT ACTTCTCGCA 
CCTCCTGCAA GAAAAGGGGC GCCCCTCCCA AAATACAAGA CTCTTCCTCC AATCTGTGGC 
AGCAAAGCCG TCATCAATTT ATACGACAAA AATAAGAGAG CTCAAATACG ACTACAGCGA 
GAATCAGCTA ACATATGGAG AAGACCCCAA ATGCCCCAAC CTGCTCTGCT GCACCTGAGT 
GTCACTGCCC ACACCTATGA CGTTCCGGAC TTCTACACAC ACTTTCCTGA TCGATACCTT 
CAGTTGACCA GGATCAAACA AAGATTAATC AATTCTTCAA GCACACGCCG CCTTGCGAAG 
CTGTGTCCAT CTACATGTGG CGCTGAGAGA GACATCGTTG TGGACATACT CACTCCTCTT 
TACAAAGATC TCCTTGAAGA TTCGGCTTTT GTAGAGTCTT TGATGGTTTT AGCCTCCAAG 
CCTCCAATCT ACGAGGGTCA GACCTCTACA TCTCCCTCCG TCACGTTTCC ATCTTCACCT 
CAACATTCTG TTCAGTCGAA CTGGACAGTG GATGGAGAAG AAACTGCAGT ATGTTTAGGG 
AAAATTCCTC AAATCCAGGC CACAGCGGAG TCCAAGCATG TTCCTTCTGA CTTGGCTGAG 
GCTGTTTTGT TAAAGACACT CCAGAATTTA ATGATGGAAG CTGTGACTGG AGAACTGACC 
TTTTCAGCAT ATTCCCGGAG CAAAGTACTG ACTTCGTTTT CAGTGAGCAG GACGTCGTTT 
GCTGAAGGGA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023192272.1
CDS	95..5209
Translation	MLAESRCPACLASAFCRWKRSSRETDVKEPQLPDIKQCQRKSHPQRSIFLGLETTLDLTWKDWVIEREYTKTLVISNIRNKLQKLHISAPETKFFSILNPEVVILSPGTSFSIPVTFKPLQKCGYKDCIQFQAIEGSFKVYLHAVLSSHTMKVPESVMLPLCAVEHYTLTSFPLKNISKVQMSFKWNSAAPFQLSPEQGRLKPGQQCVITVDFQPLEAQVYQKQVHCTYGEDGNKANSCCTVLLQGIGKYPFLQLRISNDKEETSHGDIELDFGSVAVGHSLSKQFEIFNPSPVSVWFSLSQLPGAVAIFGPHFSWDVTSEKIVPGGSVQVLVTYSPTMVDIVSVEYLSLNYRGSLSKSQVKLIGKCMGPELCLSSTVVDFGCVEEKGSAEQTVELLNSSPVQATYQWDIDCANSVFRVSPASGTVLPYGQIKVKVSYKPTHPMPHHRRVACLILLGEPLFLDLIGTCHTNLQEPVALTFEHLVEYKSKLCYMCNSSDTLLGVPLEEEGNSATAIPPAPMEKLNEIREEHVDSLFSPSFPFVSVEPSELLFNHKTRSFIAPSFAVSQCVTFKNHTKEKLSLMWTVIQDSPFCVTPSSFELAPLKSTTALVTYEPRQINALHGGELECFAYCKMPQEDPSSEQDLPCLPCCVTVRVISHSFQPGKDHFTPSCILKPHQVVFPPLSLISYRTVLLQNNGDQPLTFCLNYRTNSDLPRSVHMSPGCGFIQPGQHQILTIGAVPTLDSPTEGFNLPLQLNADEFTKELTVVSSLKKPCVSFENSNELHFTATAVGSKSQHSHSIKNLSSVPITFQWIISERDQNLICVEPDAGKLHPNESLVQTWTFSPLTEKAYTFKPTLSFQSDDFNKSQLIFRVNGTGAAGTIEAGKELLDMGESLVGSCRSFNIPIVNNCSCPISFHLSVQLEENPPDSEDNSALHLDVETGTIASHSTVLIGSTLRLDAQGWYRWAISYQITDGNGFGVCPPEKLCEVRAKGVFPTLQVVDACSSGSVDRLCKQYLWKLFSLDSFNEHLLSTPCPAEITFKAPTKHRQNSVSSFPQVILDFNFGAGPLHSDPSDFMLMFWNPGSISVNWTFLFPEDQEMKLEDWAMTGQFSNKELQEMKVQDNQLFRVTPRSGTLLPGQKCPVHFCYSHDFIGIDRLPVLFKLSLGRVFLLNFQGVTLNRNEPYLHFASNHHVFKPVAIRDSSPPRQIYELYNGGAVTVHYEVDQVVLSQLQMENYDHPVLSCLSPQGEVLPGNKAMLEWIFSPLEAKMYHVDVPIHIREKDSVIINFEGRGMNALSTSANISIEYSDAKTQEHRAQKGPFPGQVAWLSKDSVFIGDIPVCTKSSRIMFITSLSCTDTVYFVWEIPTQSNQQVVQIHPERGCLGPGECAMCILTFVSDFPTVYQLDLICQVTQNAALIHYHKALQCWEEEKKRQQNEFTITDKIPSRTQRVLVDEILLAPPARKGAPLPKYKTLPPICGSKAVINLYDKNKRAQIRLQRESANIWRRPQMPQPALLHLSVTAHTYDVPDFYTHFPDRYLQLTRIKQRLINSSSTRRLAKLCPSTCGAERDIVVDILTPLYKDLLEDSAFVESLMVLASKPPIYEGQTSTSPSVTFPSSPQHSVQSNWTVDGEETAVCLGKIPQIQATAESKHVPSDLAEAVLLKTLQNLMMEAVTGELTFSAYSRSKVLTSFSVSRTSFAEGS

Target ORF information:

RefSeq Version	XM_023336504.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus cilia and flagella associated protein 65 (cfap65), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023336504.1

ORF Insert Sequence:

ATGTTAGCTG AATCCCGCTG TCCTGCCTGT TTGGCGTCTG CTTTTTGTCG ATGGAAGCGC 
TCCAGCAGAG AGACTGACGT TAAAGAACCC CAACTCCCTG ATATAAAACA ATGTCAAAGA 
AAATCCCATC CCCAAAGAAG CATCTTCTTA GGCCTAGAAA CCACATTAGA TCTGACATGG 
AAGGACTGGG TCATTGAACG AGAGTACACC AAGACACTTG TGATAAGCAA CATCCGTAAT 
AAGCTGCAGA AGCTGCACAT TAGTGCTCCA GAGACCAAGT TTTTCTCAAT TTTGAATCCT 
GAAGTAGTTA TTCTCAGCCC AGGGACTTCA TTCTCCATAC CGGTCACTTT TAAACCTCTC 
CAAAAGTGTG GGTATAAGGA CTGCATTCAG TTTCAAGCTA TTGAAGGAAG CTTTAAAGTA 
TACCTGCATG CTGTGCTTTC AAGTCATACA ATGAAGGTGC CTGAATCTGT GATGCTGCCC 
CTATGTGCTG TTGAGCACTA CACACTAACC AGTTTCCCAC TAAAAAATAT CAGTAAGGTT 
CAGATGTCAT TTAAATGGAA TAGTGCAGCT CCTTTCCAAC TCAGTCCAGA ACAAGGCCGG 
CTAAAGCCCG GTCAGCAGTG TGTCATCACG GTGGACTTTC AGCCACTAGA GGCTCAGGTG 
TACCAGAAGC AGGTGCACTG CACATATGGA GAAGATGGCA ACAAAGCTAA TAGCTGCTGC 
ACTGTGCTTT TACAAGGGAT AGGAAAATAT CCATTTCTTC AATTAAGAAT TTCCAATGAC 
AAAGAGGAAA CCAGCCATGG TGATATTGAG TTGGACTTTG GGTCTGTGGC TGTTGGTCAC 
AGTTTGAGTA AACAATTTGA AATCTTCAAT CCTTCTCCTG TTAGTGTGTG GTTTTCTCTC 
TCACAACTGC CTGGAGCGGT AGCCATCTTT GGACCACACT TCAGTTGGGA TGTCACAAGT 
GAGAAAATAG TGCCTGGTGG TTCGGTCCAG GTTTTGGTTA CTTATTCTCC AACTATGGTC 
GACATAGTCT CTGTGGAGTA CTTGTCTTTG AATTATAGAG GGTCACTCAG TAAAAGTCAG 
GTCAAACTCA TAGGAAAGTG TATGGGTCCC GAACTCTGTT TGTCCTCCAC TGTGGTCGAC 
TTTGGGTGTG TTGAAGAAAA AGGATCAGCA GAACAAACAG TGGAACTGCT GAACTCTTCC 
CCTGTTCAGG CTACCTACCA ATGGGACATA GACTGTGCAA ACAGTGTTTT TAGAGTTTCA 
CCAGCAAGTG GCACTGTGCT TCCATATGGT CAAATCAAAG TCAAGGTCTC CTACAAACCC 
ACACACCCTA TGCCACACCA CAGAAGAGTG GCATGTCTTA TACTCCTCGG GGAGCCTCTG 
TTTCTGGACT TGATTGGTAC ATGCCACACA AATCTTCAGG AGCCAGTAGC ATTAACATTT 
GAGCACTTGG TTGAGTACAA GTCTAAGTTG TGCTATATGT GTAACTCATC AGACACACTG 
CTGGGGGTGC CTTTAGAAGA GGAAGGAAAT AGTGCTACTG CCATTCCTCC AGCACCTATG 
GAGAAATTGA ATGAAATCAG GGAAGAACAC GTGGATTCTC TCTTCTCACC ATCATTTCCA 
TTTGTATCTG TTGAACCAAG TGAACTGCTG TTTAATCACA AAACAAGATC CTTTATCGCT 
CCATCATTTG CTGTCTCACA GTGTGTTACC TTTAAAAATC ACACCAAAGA GAAACTCAGC 
CTCATGTGGA CCGTTATCCA AGATTCCCCT TTCTGTGTTA CTCCTTCGTC ATTTGAGTTG 
GCCCCACTGA AATCTACCAC TGCGCTTGTG ACATATGAAC CAAGACAGAT CAATGCCCTG 
CATGGAGGAG AGCTTGAGTG CTTTGCATAC TGTAAGATGC CACAGGAGGA TCCTTCCTCA 
GAACAAGACC TTCCATGTCT GCCTTGTTGT GTGACTGTCA GAGTCATTAG CCACTCTTTT 
CAGCCAGGGA AAGATCACTT CACTCCTTCC TGTATTTTGA AGCCCCACCA AGTGGTTTTT 
CCACCTCTGA GTCTTATTTC CTATCGGACC GTGCTACTGC AGAACAATGG CGATCAGCCT 
CTCACCTTCT GCCTGAACTA CAGAACAAAC AGTGACTTGC CAAGGTCTGT GCATATGTCA 
CCAGGCTGTG GTTTCATCCA GCCAGGACAA CACCAAATCC TTACCATTGG AGCTGTCCCC 
ACTCTGGATA GTCCCACAGA GGGGTTTAAT TTACCATTGC AGCTTAATGC AGATGAATTC 
ACGAAGGAAC TGACAGTTGT GAGCTCCTTA AAAAAGCCAT GTGTGTCTTT TGAAAATAGC 
AACGAATTAC ATTTCACTGC TACAGCTGTC GGCTCAAAAT CACAGCACAG CCATTCCATC 
AAGAATTTAA GCAGCGTGCC CATCACGTTT CAGTGGATCA TTTCAGAGAG AGATCAGAAT 
CTTATCTGCG TTGAACCAGA TGCTGGTAAA CTGCATCCCA ATGAGAGCTT GGTCCAGACA 
TGGACATTTT CTCCTCTGAC AGAGAAAGCA TACACATTCA AACCCACTCT CAGCTTCCAG 
AGCGATGACT TTAACAAGTC GCAACTCATT TTCAGAGTGA ATGGAACTGG TGCAGCTGGC 
ACCATTGAGG CAGGGAAAGA ACTCCTAGAT ATGGGAGAGA GCTTAGTTGG AAGCTGCCGG 
TCGTTTAATA TTCCTATAGT GAACAACTGT AGCTGCCCCA TCTCTTTTCA TCTGTCTGTA 
CAGCTAGAAG AAAATCCCCC TGATTCTGAA GATAACAGTG CTCTGCATCT GGACGTTGAA 
ACGGGAACAA TTGCTTCACA TTCCACTGTG CTAATCGGTT CCACTCTTAG ATTGGATGCA 
CAAGGCTGGT ACCGCTGGGC CATCAGCTAC CAGATTACAG ATGGCAATGG CTTTGGTGTA 
TGTCCTCCTG AGAAACTGTG TGAAGTGCGA GCTAAGGGGG TGTTCCCCAC CTTACAGGTG 
GTTGATGCAT GTAGCAGTGG CAGCGTGGAC AGGCTCTGCA AGCAGTATTT GTGGAAACTC 
TTCTCATTAG ACAGTTTTAA TGAGCACCTG CTTTCCACCC CATGTCCTGC AGAAATTACT 
TTCAAAGCGC CCACCAAGCA CAGACAGAAC AGTGTCTCCA GCTTCCCTCA AGTCATATTG 
GATTTCAATT TTGGTGCTGG TCCTCTGCAT TCAGATCCCT CAGATTTTAT GTTGATGTTT 
TGGAACCCCG GTTCTATCTC TGTAAACTGG ACATTCTTAT TTCCAGAGGA CCAAGAAATG 
AAGCTGGAGG ACTGGGCTAT GACAGGACAG TTTAGCAACA AAGAACTTCA GGAAATGAAG 
GTACAAGACA ATCAACTCTT CAGAGTTACC CCTCGTTCTG GTACTTTGCT TCCAGGCCAG 
AAATGTCCAG TACACTTCTG CTACAGTCAT GACTTTATTG GAATTGACCG ACTTCCTGTT 
TTGTTCAAAC TCAGTCTTGG CAGAGTTTTT TTGCTCAACT TTCAAGGAGT GACATTGAAC 
CGAAATGAAC CATATCTACA CTTTGCTTCC AATCACCATG TATTCAAGCC TGTAGCTATT 
CGGGACTCCA GTCCTCCACG ACAGATATAT GAGTTGTACA ACGGTGGTGC GGTGACAGTA 
CATTATGAAG TGGACCAAGT TGTATTGTCA CAGCTTCAGA TGGAGAACTA TGATCACCCA 
GTCTTGAGCT GTCTCAGTCC ACAAGGAGAA GTTCTCCCTG GGAATAAAGC CATGCTCGAA 
TGGATCTTCT CTCCATTGGA GGCTAAGATG TACCATGTGG ATGTTCCCAT CCACATCCGG 
GAAAAGGATT CGGTAATTAT AAACTTTGAG GGACGTGGGA TGAATGCATT ATCAACAAGC 
GCCAATATCT CCATTGAATA CAGCGATGCT AAGACTCAAG AACACCGTGC CCAGAAGGGG 
CCATTCCCAG GACAGGTGGC TTGGTTGTCC AAGGATAGTG TCTTTATTGG CGACATCCCT 
GTTTGCACAA AGTCTTCGAG AATCATGTTC ATCACCAGTT TGTCTTGCAC AGATACTGTG 
TACTTTGTCT GGGAGATTCC TACACAGAGC AACCAACAAG TGGTACAGAT CCATCCAGAA 
CGAGGCTGTC TTGGTCCAGG AGAGTGTGCT ATGTGCATCC TGACCTTCGT TTCTGATTTC 
CCCACTGTTT ATCAGCTAGA TCTCATATGT CAGGTCACTC AGAACGCTGC ACTCATCCAT 
TATCACAAAG CCTTGCAATG CTGGGAGGAA GAAAAGAAAC GACAGCAAAA CGAATTCACA 
ATCACAGACA AGATCCCTTC CCGCACTCAA AGAGTTCTAG TAGATGAAAT ACTTCTCGCA 
CCTCCTGCAA GAAAAGGGGC GCCCCTCCCA AAATACAAGA CTCTTCCTCC AATCTGTGGC 
AGCAAAGCCG TCATCAATTT ATACGACAAA AATAAGAGAG CTCAAATACG ACTACAGCGA 
GAATCAGCTA ACATATGGAG AAGACCCCAA ATGCCCCAAC CTGCTCTGCT GCACCTGAGT 
GTCACTGCCC ACACCTATGA CGTTCCGGAC TTCTACACAC ACTTTCCTGA TCGATACCTT 
CAGTTGACCA GGATCAAACA AAGATTAATC AATTCTTCAA GCACACGCCG CCTTGCGAAG 
CTGTGTCCAT CTACATGTGG CGCTGAGAGA GACATCGTTG TGGACATACT CACTCCTCTT 
TACAAAGATC TCCTTGAAGA TTCGGCTTTT GTAGAGTCTT TGATGGTTTT AGCCTCCAAG 
CCTCCAATCT ACGAGGGTCA GACCTCTACA TCTCCCTCCG TCACGTTTCC ATCTTCACCT 
CAACATTCTG TTCAGTCGAA CTGGACAGTG GATGGAGAAG AAACTGCAGT ATGTTTAGGG 
AAAATTCCTC AAATCCAGGC CACAGCGGAG TCCAAGCATG TTCCTTCTGA CTTGGCTGAG 
GCTGTTTTGT TAAAGACACT CCAGAATTTA ATGATGGAAG CTGTGACTGG AGAACTGACC 
TTTTCAGCAT ATTCCCGGAG CAAAGTACTG ACTTCGTTTT CAGTGAGCAG GACGTCGTTT 
GCTGAAGGGA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cfap65 ( XM_023336505.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023192273.1 Xiphophorus maculatus cilia and flagella associated protein 65 (cfap65), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXi40282
Clone ID Related Accession (Same CDS sequence)	XM_023336505.1
Accession Version	XM_023336505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	cilia- and flagella-associated protein 65 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036449.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGCTG AATCCCGCTG TCCTGCCTGT TTGGCGTCTG CTTTTTGTCG ATGGAAGCGC 
TCCAGCAGAG AGACTGACGT TAAAGAACCC CAACTCCCTG ATATAAAACA ATGTCAAAGA 
AAATCCCATC CCCAAAGAAG CATCTTCTTA GGCCTAGAAA CCACATTAGA TCTGACATGG 
AAGGACTGGG TCATTGAACG AGAGTACACC AAGACACTTG TGATAAGCAA CATCCGTAAT 
AAGCTGCAGA AGCTGCACAT TAGTGCTCCA GAGACCAAGT TTTTCTCAAT TTTGAATCCT 
GAAGTAGTTA TTCTCAGCCC AGGGACTTCA TTCTCCATAC CGGTCACTTT TAAACCTCTC 
CAAAAGTGTG GGTATAAGGA CTGCATTCAG TTTCAAGCTA TTGAAGGAAG CTTTAAAGTA 
TACCTGCATG CTGTGCTTTC AAGTCATACA ATGAAGGTGC CTGAATCTGT GATGCTGCCC 
CTATGTGCTG TTGAGCACTA CACACTAACC AGTTTCCCAC TAAAAAATAT CAGTAAGGTT 
CAGATGTCAT TTAAATGGAA TAGTGCAGCT CCTTTCCAAC TCAGTCCAGA ACAAGGCCGG 
CTAAAGCCCG GTCAGCAGTG TGTCATCACG GTGGACTTTC AGCCACTAGA GGCTCAGGTG 
TACCAGAAGC AGGTGCACTG CACATATGGA GAAGATGGCA ACAAAGCTAA TAGCTGCTGC 
ACTGTGCTTT TACAAGGGAT AGGAAAATAT CCATTTCTTC AATTAAGAAT TTCCAATGAC 
AAAGAGGAAA CCAGCCATGG TGATATTGAG TTGGACTTTG GGTCTGTGGC TGTTGGTCAC 
AGTTTGAGTA AACAATTTGA AATCTTCAAT CCTTCTCCTG TTAGTGTGTG GTTTTCTCTC 
TCACAACTGC CTGGAGCGGT AGCCATCTTT GGACCACACT TCAGTTGGGA TGTCACAAGT 
GAGAAAATAG TGCCTGGTGG TTCGGTCCAG GTTTTGGTTA CTTATTCTCC AACTATGGTC 
GACATAGTCT CTGTGGAGTA CTTGTCTTTG AATTATAGAG GGTCACTCAG TAAAAGTCAG 
GTCAAACTCA TAGGAAAGTG TATGGGTCCC GAACTCTGTT TGTCCTCCAC TGTGGTCGAC 
TTTGGGTGTG TTGAAGAAAA AGGATCAGCA GAACAAACAG TGGAACTGCT GAACTCTTCC 
CCTGTTCAGG CTACCTACCA ATGGGACATA GACTGTGCAA ACAGTGTTTT TAGAGTTTCA 
CCAGCAAGTG GCACTGTGCT TCCATATGGT CAAATCAAAG TCAAGGTCTC CTACAAACCC 
ACACACCCTA TGCCACACCA CAGAAGAGTG GCATGTCTTA TACTCCTCGG GGAGCCTCTG 
TTTCTGGACT TGATTGGTAC ATGCCACACA AATCTTCAGG AGCCAGTAGC ATTAACATTT 
GAGCACTTGG TTGAGTACAA GTCTAAGTTG TGCTATATGT GTAACTCATC AGACACACTG 
CTGGGGGTGC CTTTAGAAGA GGAAGGAAAT AGTGCTACTG CCATTCCTCC AGCACCTATG 
GAGAAATTGA ATGAAATCAG GGAAGAACAC GTGGATTCTC TCTTCTCACC ATCATTTCCA 
TTTGTATCTG TTGAACCAAG TGAACTGCTG TTTAATCACA AAACAAGATC CTTTATCGCT 
CCATCATTTG CTGTCTCACA GTGTGTTACC TTTAAAAATC ACACCAAAGA GAAACTCAGC 
CTCATGTGGA CCGTTATCCA AGATTCCCCT TTCTGTGTTA CTCCTTCGTC ATTTGAGTTG 
GCCCCACTGA AATCTACCAC TGCGCTTGTG ACATATGAAC CAAGACAGAT CAATGCCCTG 
CATGGAGGAG AGCTTGAGTG CTTTGCATAC TGTAAGATGC CACAGGAGGA TCCTTCCTCA 
GAACAAGACC TTCCATGTCT GCCTTGTTGT GTGACTGTCA GAGTCATTAG CCACTCTTTT 
CAGCCAGGGA AAGATCACTT CACTCCTTCC TGTATTTTGA AGCCCCACCA AGTGGTTTTT 
CCACCTCTGA GTCTTATTTC CTATCGGACC GTGCTACTGC AGAACAATGG CGATCAGCCT 
CTCACCTTCT GCCTGAACTA CAGAACAAAC AGTGACTTGC CAAGGTCTGT GCATATGTCA 
CCAGGCTGTG GTTTCATCCA GCCAGGACAA CACCAAATCC TTACCATTGG AGCTGTCCCC 
ACTCTGGATA GTCCCACAGA GGGGTTTAAT TTACCATTGC AGCTTAATGC AGATGAATTC 
ACGAAGGAAC TGACAGTTGT GAGCTCCTTA AAAAAGCCAT GTGTGTCTTT TGAAAATAGC 
AACGAATTAC ATTTCACTGC TACAGCTGTC GGCTCAAAAT CACAGCACAG CCATTCCATC 
AAGAATTTAA GCAGCGTGCC CATCACGTTT CAGTGGATCA TTTCAGAGAG AGATCAGAAT 
CTTATCTGCG TTGAACCAGA TGCTGGTAAA CTGCATCCCA ATGAGAGCTT GGTCCAGACA 
TGGACATTTT CTCCTCTGAC AGAGAAAGCA TACACATTCA AACCCACTCT CAGCTTCCAG 
AGCGATGACT TTAACAAGTC GCAACTCATT TTCAGAGTGA ATGGAACTGG TGCAGCTGGC 
ACCATTGAGG CAGGGAAAGA ACTCCTAGAT ATGGGAGAGA GCTTAGTTGG AAGCTGCCGG 
TCGTTTAATA TTCCTATAGT GAACAACTGT AGCTGCCCCA TCTCTTTTCA TCTGTCTGTA 
CAGCTAGAAG AAAATCCCCC TGATTCTGAA GATAACAGTG CTCTGCATCT GGACGTTGAA 
ACGGGAACAA TTGCTTCACA TTCCACTGTG CTAATCGGTT CCACTCTTAG ATTGGATGCA 
CAAGGCTGGT ACCGCTGGGC CATCAGCTAC CAGATTACAG ATGGCAATGG CTTTGGTGTA 
TGTCCTCCTG AGAAACTGTG TGAAGTGCGA GCTAAGGGGG TGTTCCCCAC CTTACAGGTG 
GTTGATGCAT GTAGCAGTGG CAGCGTGGAC AGGCTCTGCA AGCAGTATTT GTGGAAACTC 
TTCTCATTAG ACAGTTTTAA TGAGCACCTG CTTTCCACCC CATGTCCTGC AGAAATTACT 
TTCAAAGCGC CCACCAAGCA CAGACAGAAC AGTGTCTCCA GCTTCCCTCA AGTCATATTG 
GATTTCAATT TTGGTGCTGG TCCTCTGCAT TCAGATCCCT CAGATTTTAT GTTGATGTTT 
TGGAACCCCG GTTCTATCTC TGTAAACTGG ACATTCTTAT TTCCAGAGGA CCAAGAAATG 
AAGCTGGAGG ACTGGGCTAT GACAGGACAG TTTAGCAACA AAGAACTTCA GGAAATGAAG 
GTACAAGACA ATCAACTCTT CAGAGTTACC CCTCGTTCTG GTACTTTGCT TCCAGGCCAG 
AAATGTCCAG TACACTTCTG CTACAGTCAT GACTTTATTG GAATTGACCG ACTTCCTGTT 
TTGTTCAAAC TCAGTCTTGG CAGAGTTTTT TTGCTCAACT TTCAAGGAGT GACATTGAAC 
CGAAATGAAC CATATCTACA CTTTGCTTCC AATCACCATG TATTCAAGCC TGTAGCTATT 
CGGGACTCCA GTCCTCCACG ACAGATATAT GAGTTGTACA ACGGTGGTGC GGTGACAGTA 
CATTATGAAG TGGACCAAGT TGTATTGTCA CAGCTTCAGA TGGAGAACTA TGATCACCCA 
GTCTTGAGCT GTCTCAGTCC ACAAGGAGAA GTTCTCCCTG GGAATAAAGC CATGCTCGAA 
TGGATCTTCT CTCCATTGGA GGCTAAGATG TACCATGTGG ATGTTCCCAT CCACATCCGG 
GAAAAGGATT CGGTAATTAT AAACTTTGAG GGACGTGGGA TGAATGCATT ATCAACAAGC 
GCCAATATCT CCATTGAATA CAGCGATGCT AAGACTCAAG AACACCGTGC CCAGAAGGGG 
CCATTCCCAG GACAGGTGGC TTGGTTGTCC AAGGATAGTG TCTTTATTGG CGACATCCCT 
GTTTGCACAA AGTCTTCGAG AATCATGTTC ATCACCAGTT TGTCTTGCAC AGATACTGTG 
TACTTTGTCT GGGAGATTCC TACACAGAGC AACCAACAAG TGGTACAGAT CCATCCAGAA 
CGAGGCTGTC TTGGTCCAGG AGAGTGTGCT ATGTGCATCC TGACCTTCGT TTCTGATTTC 
CCCACTGTTT ATCAGCTAGA TCTCATATGT CAGGTCACTC AGAACGCTGC ACTCATCCAT 
TATCACAAAG CCTTGCAATG CTGGGAGGAA GAAAAGAAAC GACAGCAAAA CGAATTCACA 
ATCACAGACA AGATCCCTTC CCGCACTCAA AGAGTTCTAG TAGATGAAAT ACTTCTCGCA 
CCTCCTGCAA GAAAAGGGGC GCCCCTCCCA AAATACAAGA CTCTTCCTCC AATCTGTGGC 
AGCAAAGCCG TCATCAATTT ATACGACAAA AATAAGAGAG CTCAAATACG ACTACAGCGA 
GAATCAGCTA ACATATGGAG AAGACCCCAA ATGCCCCAAC CTGCTCTGCT GCACCTGAGT 
GTCACTGCCC ACACCTATGA CGTTCCGGAC TTCTACACAC ACTTTCCTGA TCGATACCTT 
CAGTTGACCA GGATCAAACA AAGATTAATC AATTCTTCAA GCACACGCCG CCTTGCGAAG 
CTGTGTCCAT CTACATGTGG CGCTGAGAGA GACATCGTTG TGGACATACT CACTCCTCTT 
TACAAAGATC TCCTTGAAGA TTCGGCTTTT GTAGAGTCTT TGATGGTTTT AGCCTCCAAG 
CCTCCAATCT ACGAGGGTCA GACCTCTACA TCTCCCTCCG TCACGTTTCC ATCTTCACCT 
CAACATTCTG TTCAGTCGAA CTGGACAGTG GATGGAGAAG AAACTGCAGT ATGTTTAGGG 
AAAATTCCTC AAATCCAGGC CACAGCGGAG TCCAAGCATG TTCCTTCTGA CTTGGCTGAG 
GCTGTTTTGT TAAAGACACT CCAGAATTTA ATGATGGAAG CTGTGACTGG AGAACTGACC 
TTTTCAGCAT ATTCCCGGAG CAAAGTACTG ACTTCGTTTT CAGTGAGGAC GTCGTTTGCT 
GAAGGGAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023192273.1
CDS	95..5206
Translation	MLAESRCPACLASAFCRWKRSSRETDVKEPQLPDIKQCQRKSHPQRSIFLGLETTLDLTWKDWVIEREYTKTLVISNIRNKLQKLHISAPETKFFSILNPEVVILSPGTSFSIPVTFKPLQKCGYKDCIQFQAIEGSFKVYLHAVLSSHTMKVPESVMLPLCAVEHYTLTSFPLKNISKVQMSFKWNSAAPFQLSPEQGRLKPGQQCVITVDFQPLEAQVYQKQVHCTYGEDGNKANSCCTVLLQGIGKYPFLQLRISNDKEETSHGDIELDFGSVAVGHSLSKQFEIFNPSPVSVWFSLSQLPGAVAIFGPHFSWDVTSEKIVPGGSVQVLVTYSPTMVDIVSVEYLSLNYRGSLSKSQVKLIGKCMGPELCLSSTVVDFGCVEEKGSAEQTVELLNSSPVQATYQWDIDCANSVFRVSPASGTVLPYGQIKVKVSYKPTHPMPHHRRVACLILLGEPLFLDLIGTCHTNLQEPVALTFEHLVEYKSKLCYMCNSSDTLLGVPLEEEGNSATAIPPAPMEKLNEIREEHVDSLFSPSFPFVSVEPSELLFNHKTRSFIAPSFAVSQCVTFKNHTKEKLSLMWTVIQDSPFCVTPSSFELAPLKSTTALVTYEPRQINALHGGELECFAYCKMPQEDPSSEQDLPCLPCCVTVRVISHSFQPGKDHFTPSCILKPHQVVFPPLSLISYRTVLLQNNGDQPLTFCLNYRTNSDLPRSVHMSPGCGFIQPGQHQILTIGAVPTLDSPTEGFNLPLQLNADEFTKELTVVSSLKKPCVSFENSNELHFTATAVGSKSQHSHSIKNLSSVPITFQWIISERDQNLICVEPDAGKLHPNESLVQTWTFSPLTEKAYTFKPTLSFQSDDFNKSQLIFRVNGTGAAGTIEAGKELLDMGESLVGSCRSFNIPIVNNCSCPISFHLSVQLEENPPDSEDNSALHLDVETGTIASHSTVLIGSTLRLDAQGWYRWAISYQITDGNGFGVCPPEKLCEVRAKGVFPTLQVVDACSSGSVDRLCKQYLWKLFSLDSFNEHLLSTPCPAEITFKAPTKHRQNSVSSFPQVILDFNFGAGPLHSDPSDFMLMFWNPGSISVNWTFLFPEDQEMKLEDWAMTGQFSNKELQEMKVQDNQLFRVTPRSGTLLPGQKCPVHFCYSHDFIGIDRLPVLFKLSLGRVFLLNFQGVTLNRNEPYLHFASNHHVFKPVAIRDSSPPRQIYELYNGGAVTVHYEVDQVVLSQLQMENYDHPVLSCLSPQGEVLPGNKAMLEWIFSPLEAKMYHVDVPIHIREKDSVIINFEGRGMNALSTSANISIEYSDAKTQEHRAQKGPFPGQVAWLSKDSVFIGDIPVCTKSSRIMFITSLSCTDTVYFVWEIPTQSNQQVVQIHPERGCLGPGECAMCILTFVSDFPTVYQLDLICQVTQNAALIHYHKALQCWEEEKKRQQNEFTITDKIPSRTQRVLVDEILLAPPARKGAPLPKYKTLPPICGSKAVINLYDKNKRAQIRLQRESANIWRRPQMPQPALLHLSVTAHTYDVPDFYTHFPDRYLQLTRIKQRLINSSSTRRLAKLCPSTCGAERDIVVDILTPLYKDLLEDSAFVESLMVLASKPPIYEGQTSTSPSVTFPSSPQHSVQSNWTVDGEETAVCLGKIPQIQATAESKHVPSDLAEAVLLKTLQNLMMEAVTGELTFSAYSRSKVLTSFSVRTSFAEGS

Target ORF information:

RefSeq Version	XM_023336505.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus cilia and flagella associated protein 65 (cfap65), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023336505.1

ORF Insert Sequence:

ATGTTAGCTG AATCCCGCTG TCCTGCCTGT TTGGCGTCTG CTTTTTGTCG ATGGAAGCGC 
TCCAGCAGAG AGACTGACGT TAAAGAACCC CAACTCCCTG ATATAAAACA ATGTCAAAGA 
AAATCCCATC CCCAAAGAAG CATCTTCTTA GGCCTAGAAA CCACATTAGA TCTGACATGG 
AAGGACTGGG TCATTGAACG AGAGTACACC AAGACACTTG TGATAAGCAA CATCCGTAAT 
AAGCTGCAGA AGCTGCACAT TAGTGCTCCA GAGACCAAGT TTTTCTCAAT TTTGAATCCT 
GAAGTAGTTA TTCTCAGCCC AGGGACTTCA TTCTCCATAC CGGTCACTTT TAAACCTCTC 
CAAAAGTGTG GGTATAAGGA CTGCATTCAG TTTCAAGCTA TTGAAGGAAG CTTTAAAGTA 
TACCTGCATG CTGTGCTTTC AAGTCATACA ATGAAGGTGC CTGAATCTGT GATGCTGCCC 
CTATGTGCTG TTGAGCACTA CACACTAACC AGTTTCCCAC TAAAAAATAT CAGTAAGGTT 
CAGATGTCAT TTAAATGGAA TAGTGCAGCT CCTTTCCAAC TCAGTCCAGA ACAAGGCCGG 
CTAAAGCCCG GTCAGCAGTG TGTCATCACG GTGGACTTTC AGCCACTAGA GGCTCAGGTG 
TACCAGAAGC AGGTGCACTG CACATATGGA GAAGATGGCA ACAAAGCTAA TAGCTGCTGC 
ACTGTGCTTT TACAAGGGAT AGGAAAATAT CCATTTCTTC AATTAAGAAT TTCCAATGAC 
AAAGAGGAAA CCAGCCATGG TGATATTGAG TTGGACTTTG GGTCTGTGGC TGTTGGTCAC 
AGTTTGAGTA AACAATTTGA AATCTTCAAT CCTTCTCCTG TTAGTGTGTG GTTTTCTCTC 
TCACAACTGC CTGGAGCGGT AGCCATCTTT GGACCACACT TCAGTTGGGA TGTCACAAGT 
GAGAAAATAG TGCCTGGTGG TTCGGTCCAG GTTTTGGTTA CTTATTCTCC AACTATGGTC 
GACATAGTCT CTGTGGAGTA CTTGTCTTTG AATTATAGAG GGTCACTCAG TAAAAGTCAG 
GTCAAACTCA TAGGAAAGTG TATGGGTCCC GAACTCTGTT TGTCCTCCAC TGTGGTCGAC 
TTTGGGTGTG TTGAAGAAAA AGGATCAGCA GAACAAACAG TGGAACTGCT GAACTCTTCC 
CCTGTTCAGG CTACCTACCA ATGGGACATA GACTGTGCAA ACAGTGTTTT TAGAGTTTCA 
CCAGCAAGTG GCACTGTGCT TCCATATGGT CAAATCAAAG TCAAGGTCTC CTACAAACCC 
ACACACCCTA TGCCACACCA CAGAAGAGTG GCATGTCTTA TACTCCTCGG GGAGCCTCTG 
TTTCTGGACT TGATTGGTAC ATGCCACACA AATCTTCAGG AGCCAGTAGC ATTAACATTT 
GAGCACTTGG TTGAGTACAA GTCTAAGTTG TGCTATATGT GTAACTCATC AGACACACTG 
CTGGGGGTGC CTTTAGAAGA GGAAGGAAAT AGTGCTACTG CCATTCCTCC AGCACCTATG 
GAGAAATTGA ATGAAATCAG GGAAGAACAC GTGGATTCTC TCTTCTCACC ATCATTTCCA 
TTTGTATCTG TTGAACCAAG TGAACTGCTG TTTAATCACA AAACAAGATC CTTTATCGCT 
CCATCATTTG CTGTCTCACA GTGTGTTACC TTTAAAAATC ACACCAAAGA GAAACTCAGC 
CTCATGTGGA CCGTTATCCA AGATTCCCCT TTCTGTGTTA CTCCTTCGTC ATTTGAGTTG 
GCCCCACTGA AATCTACCAC TGCGCTTGTG ACATATGAAC CAAGACAGAT CAATGCCCTG 
CATGGAGGAG AGCTTGAGTG CTTTGCATAC TGTAAGATGC CACAGGAGGA TCCTTCCTCA 
GAACAAGACC TTCCATGTCT GCCTTGTTGT GTGACTGTCA GAGTCATTAG CCACTCTTTT 
CAGCCAGGGA AAGATCACTT CACTCCTTCC TGTATTTTGA AGCCCCACCA AGTGGTTTTT 
CCACCTCTGA GTCTTATTTC CTATCGGACC GTGCTACTGC AGAACAATGG CGATCAGCCT 
CTCACCTTCT GCCTGAACTA CAGAACAAAC AGTGACTTGC CAAGGTCTGT GCATATGTCA 
CCAGGCTGTG GTTTCATCCA GCCAGGACAA CACCAAATCC TTACCATTGG AGCTGTCCCC 
ACTCTGGATA GTCCCACAGA GGGGTTTAAT TTACCATTGC AGCTTAATGC AGATGAATTC 
ACGAAGGAAC TGACAGTTGT GAGCTCCTTA AAAAAGCCAT GTGTGTCTTT TGAAAATAGC 
AACGAATTAC ATTTCACTGC TACAGCTGTC GGCTCAAAAT CACAGCACAG CCATTCCATC 
AAGAATTTAA GCAGCGTGCC CATCACGTTT CAGTGGATCA TTTCAGAGAG AGATCAGAAT 
CTTATCTGCG TTGAACCAGA TGCTGGTAAA CTGCATCCCA ATGAGAGCTT GGTCCAGACA 
TGGACATTTT CTCCTCTGAC AGAGAAAGCA TACACATTCA AACCCACTCT CAGCTTCCAG 
AGCGATGACT TTAACAAGTC GCAACTCATT TTCAGAGTGA ATGGAACTGG TGCAGCTGGC 
ACCATTGAGG CAGGGAAAGA ACTCCTAGAT ATGGGAGAGA GCTTAGTTGG AAGCTGCCGG 
TCGTTTAATA TTCCTATAGT GAACAACTGT AGCTGCCCCA TCTCTTTTCA TCTGTCTGTA 
CAGCTAGAAG AAAATCCCCC TGATTCTGAA GATAACAGTG CTCTGCATCT GGACGTTGAA 
ACGGGAACAA TTGCTTCACA TTCCACTGTG CTAATCGGTT CCACTCTTAG ATTGGATGCA 
CAAGGCTGGT ACCGCTGGGC CATCAGCTAC CAGATTACAG ATGGCAATGG CTTTGGTGTA 
TGTCCTCCTG AGAAACTGTG TGAAGTGCGA GCTAAGGGGG TGTTCCCCAC CTTACAGGTG 
GTTGATGCAT GTAGCAGTGG CAGCGTGGAC AGGCTCTGCA AGCAGTATTT GTGGAAACTC 
TTCTCATTAG ACAGTTTTAA TGAGCACCTG CTTTCCACCC CATGTCCTGC AGAAATTACT 
TTCAAAGCGC CCACCAAGCA CAGACAGAAC AGTGTCTCCA GCTTCCCTCA AGTCATATTG 
GATTTCAATT TTGGTGCTGG TCCTCTGCAT TCAGATCCCT CAGATTTTAT GTTGATGTTT 
TGGAACCCCG GTTCTATCTC TGTAAACTGG ACATTCTTAT TTCCAGAGGA CCAAGAAATG 
AAGCTGGAGG ACTGGGCTAT GACAGGACAG TTTAGCAACA AAGAACTTCA GGAAATGAAG 
GTACAAGACA ATCAACTCTT CAGAGTTACC CCTCGTTCTG GTACTTTGCT TCCAGGCCAG 
AAATGTCCAG TACACTTCTG CTACAGTCAT GACTTTATTG GAATTGACCG ACTTCCTGTT 
TTGTTCAAAC TCAGTCTTGG CAGAGTTTTT TTGCTCAACT TTCAAGGAGT GACATTGAAC 
CGAAATGAAC CATATCTACA CTTTGCTTCC AATCACCATG TATTCAAGCC TGTAGCTATT 
CGGGACTCCA GTCCTCCACG ACAGATATAT GAGTTGTACA ACGGTGGTGC GGTGACAGTA 
CATTATGAAG TGGACCAAGT TGTATTGTCA CAGCTTCAGA TGGAGAACTA TGATCACCCA 
GTCTTGAGCT GTCTCAGTCC ACAAGGAGAA GTTCTCCCTG GGAATAAAGC CATGCTCGAA 
TGGATCTTCT CTCCATTGGA GGCTAAGATG TACCATGTGG ATGTTCCCAT CCACATCCGG 
GAAAAGGATT CGGTAATTAT AAACTTTGAG GGACGTGGGA TGAATGCATT ATCAACAAGC 
GCCAATATCT CCATTGAATA CAGCGATGCT AAGACTCAAG AACACCGTGC CCAGAAGGGG 
CCATTCCCAG GACAGGTGGC TTGGTTGTCC AAGGATAGTG TCTTTATTGG CGACATCCCT 
GTTTGCACAA AGTCTTCGAG AATCATGTTC ATCACCAGTT TGTCTTGCAC AGATACTGTG 
TACTTTGTCT GGGAGATTCC TACACAGAGC AACCAACAAG TGGTACAGAT CCATCCAGAA 
CGAGGCTGTC TTGGTCCAGG AGAGTGTGCT ATGTGCATCC TGACCTTCGT TTCTGATTTC 
CCCACTGTTT ATCAGCTAGA TCTCATATGT CAGGTCACTC AGAACGCTGC ACTCATCCAT 
TATCACAAAG CCTTGCAATG CTGGGAGGAA GAAAAGAAAC GACAGCAAAA CGAATTCACA 
ATCACAGACA AGATCCCTTC CCGCACTCAA AGAGTTCTAG TAGATGAAAT ACTTCTCGCA 
CCTCCTGCAA GAAAAGGGGC GCCCCTCCCA AAATACAAGA CTCTTCCTCC AATCTGTGGC 
AGCAAAGCCG TCATCAATTT ATACGACAAA AATAAGAGAG CTCAAATACG ACTACAGCGA 
GAATCAGCTA ACATATGGAG AAGACCCCAA ATGCCCCAAC CTGCTCTGCT GCACCTGAGT 
GTCACTGCCC ACACCTATGA CGTTCCGGAC TTCTACACAC ACTTTCCTGA TCGATACCTT 
CAGTTGACCA GGATCAAACA AAGATTAATC AATTCTTCAA GCACACGCCG CCTTGCGAAG 
CTGTGTCCAT CTACATGTGG CGCTGAGAGA GACATCGTTG TGGACATACT CACTCCTCTT 
TACAAAGATC TCCTTGAAGA TTCGGCTTTT GTAGAGTCTT TGATGGTTTT AGCCTCCAAG 
CCTCCAATCT ACGAGGGTCA GACCTCTACA TCTCCCTCCG TCACGTTTCC ATCTTCACCT 
CAACATTCTG TTCAGTCGAA CTGGACAGTG GATGGAGAAG AAACTGCAGT ATGTTTAGGG 
AAAATTCCTC AAATCCAGGC CACAGCGGAG TCCAAGCATG TTCCTTCTGA CTTGGCTGAG 
GCTGTTTTGT TAAAGACACT CCAGAATTTA ATGATGGAAG CTGTGACTGG AGAACTGACC 
TTTTCAGCAT ATTCCCGGAG CAAAGTACTG ACTTCGTTTT CAGTGAGGAC GTCGTTTGCT 
GAAGGGAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cfap65 ( XM_023336506.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023192274.1 Xiphophorus maculatus cilia and flagella associated protein 65 (cfap65), transcript variant X3, mRNA.

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CloneID	OXi40283
Clone ID Related Accession (Same CDS sequence)	XM_023336506.1
Accession Version	XM_023336506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	cilia- and flagella-associated protein 65 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036449.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCCG ATTACAGAAG TTCAAAAGGT CACTGCTGTA ATGAACCCCA ACTCCCTGAT 
ATAAAACAAT GTCAAAGAAA ATCCCATCCC CAAAGAAGCA TCTTCTTAGG CCTAGAAACC 
ACATTAGATC TGACATGGAA GGACTGGGTC ATTGAACGAG AGTACACCAA GACACTTGTG 
ATAAGCAACA TCCGTAATAA GCTGCAGAAG CTGCACATTA GTGCTCCAGA GACCAAGTTT 
TTCTCAATTT TGAATCCTGA AGTAGTTATT CTCAGCCCAG GGACTTCATT CTCCATACCG 
GTCACTTTTA AACCTCTCCA AAAGTGTGGG TATAAGGACT GCATTCAGTT TCAAGCTATT 
GAAGGAAGCT TTAAAGTATA CCTGCATGCT GTGCTTTCAA GTCATACAAT GAAGGTGCCT 
GAATCTGTGA TGCTGCCCCT ATGTGCTGTT GAGCACTACA CACTAACCAG TTTCCCACTA 
AAAAATATCA GTAAGGTTCA GATGTCATTT AAATGGAATA GTGCAGCTCC TTTCCAACTC 
AGTCCAGAAC AAGGCCGGCT AAAGCCCGGT CAGCAGTGTG TCATCACGGT GGACTTTCAG 
CCACTAGAGG CTCAGGTGTA CCAGAAGCAG GTGCACTGCA CATATGGAGA AGATGGCAAC 
AAAGCTAATA GCTGCTGCAC TGTGCTTTTA CAAGGGATAG GAAAATATCC ATTTCTTCAA 
TTAAGAATTT CCAATGACAA AGAGGAAACC AGCCATGGTG ATATTGAGTT GGACTTTGGG 
TCTGTGGCTG TTGGTCACAG TTTGAGTAAA CAATTTGAAA TCTTCAATCC TTCTCCTGTT 
AGTGTGTGGT TTTCTCTCTC ACAACTGCCT GGAGCGGTAG CCATCTTTGG ACCACACTTC 
AGTTGGGATG TCACAAGTGA GAAAATAGTG CCTGGTGGTT CGGTCCAGGT TTTGGTTACT 
TATTCTCCAA CTATGGTCGA CATAGTCTCT GTGGAGTACT TGTCTTTGAA TTATAGAGGG 
TCACTCAGTA AAAGTCAGGT CAAACTCATA GGAAAGTGTA TGGGTCCCGA ACTCTGTTTG 
TCCTCCACTG TGGTCGACTT TGGGTGTGTT GAAGAAAAAG GATCAGCAGA ACAAACAGTG 
GAACTGCTGA ACTCTTCCCC TGTTCAGGCT ACCTACCAAT GGGACATAGA CTGTGCAAAC 
AGTGTTTTTA GAGTTTCACC AGCAAGTGGC ACTGTGCTTC CATATGGTCA AATCAAAGTC 
AAGGTCTCCT ACAAACCCAC ACACCCTATG CCACACCACA GAAGAGTGGC ATGTCTTATA 
CTCCTCGGGG AGCCTCTGTT TCTGGACTTG ATTGGTACAT GCCACACAAA TCTTCAGGAG 
CCAGTAGCAT TAACATTTGA GCACTTGGTT GAGTACAAGT CTAAGTTGTG CTATATGTGT 
AACTCATCAG ACACACTGCT GGGGGTGCCT TTAGAAGAGG AAGGAAATAG TGCTACTGCC 
ATTCCTCCAG CACCTATGGA GAAATTGAAT GAAATCAGGG AAGAACACGT GGATTCTCTC 
TTCTCACCAT CATTTCCATT TGTATCTGTT GAACCAAGTG AACTGCTGTT TAATCACAAA 
ACAAGATCCT TTATCGCTCC ATCATTTGCT GTCTCACAGT GTGTTACCTT TAAAAATCAC 
ACCAAAGAGA AACTCAGCCT CATGTGGACC GTTATCCAAG ATTCCCCTTT CTGTGTTACT 
CCTTCGTCAT TTGAGTTGGC CCCACTGAAA TCTACCACTG CGCTTGTGAC ATATGAACCA 
AGACAGATCA ATGCCCTGCA TGGAGGAGAG CTTGAGTGCT TTGCATACTG TAAGATGCCA 
CAGGAGGATC CTTCCTCAGA ACAAGACCTT CCATGTCTGC CTTGTTGTGT GACTGTCAGA 
GTCATTAGCC ACTCTTTTCA GCCAGGGAAA GATCACTTCA CTCCTTCCTG TATTTTGAAG 
CCCCACCAAG TGGTTTTTCC ACCTCTGAGT CTTATTTCCT ATCGGACCGT GCTACTGCAG 
AACAATGGCG ATCAGCCTCT CACCTTCTGC CTGAACTACA GAACAAACAG TGACTTGCCA 
AGGTCTGTGC ATATGTCACC AGGCTGTGGT TTCATCCAGC CAGGACAACA CCAAATCCTT 
ACCATTGGAG CTGTCCCCAC TCTGGATAGT CCCACAGAGG GGTTTAATTT ACCATTGCAG 
CTTAATGCAG ATGAATTCAC GAAGGAACTG ACAGTTGTGA GCTCCTTAAA AAAGCCATGT 
GTGTCTTTTG AAAATAGCAA CGAATTACAT TTCACTGCTA CAGCTGTCGG CTCAAAATCA 
CAGCACAGCC ATTCCATCAA GAATTTAAGC AGCGTGCCCA TCACGTTTCA GTGGATCATT 
TCAGAGAGAG ATCAGAATCT TATCTGCGTT GAACCAGATG CTGGTAAACT GCATCCCAAT 
GAGAGCTTGG TCCAGACATG GACATTTTCT CCTCTGACAG AGAAAGCATA CACATTCAAA 
CCCACTCTCA GCTTCCAGAG CGATGACTTT AACAAGTCGC AACTCATTTT CAGAGTGAAT 
GGAACTGGTG CAGCTGGCAC CATTGAGGCA GGGAAAGAAC TCCTAGATAT GGGAGAGAGC 
TTAGTTGGAA GCTGCCGGTC GTTTAATATT CCTATAGTGA ACAACTGTAG CTGCCCCATC 
TCTTTTCATC TGTCTGTACA GCTAGAAGAA AATCCCCCTG ATTCTGAAGA TAACAGTGCT 
CTGCATCTGG ACGTTGAAAC GGGAACAATT GCTTCACATT CCACTGTGCT AATCGGTTCC 
ACTCTTAGAT TGGATGCACA AGGCTGGTAC CGCTGGGCCA TCAGCTACCA GATTACAGAT 
GGCAATGGCT TTGGTGTATG TCCTCCTGAG AAACTGTGTG AAGTGCGAGC TAAGGGGGTG 
TTCCCCACCT TACAGGTGGT TGATGCATGT AGCAGTGGCA GCGTGGACAG GCTCTGCAAG 
CAGTATTTGT GGAAACTCTT CTCATTAGAC AGTTTTAATG AGCACCTGCT TTCCACCCCA 
TGTCCTGCAG AAATTACTTT CAAAGCGCCC ACCAAGCACA GACAGAACAG TGTCTCCAGC 
TTCCCTCAAG TCATATTGGA TTTCAATTTT GGTGCTGGTC CTCTGCATTC AGATCCCTCA 
GATTTTATGT TGATGTTTTG GAACCCCGGT TCTATCTCTG TAAACTGGAC ATTCTTATTT 
CCAGAGGACC AAGAAATGAA GCTGGAGGAC TGGGCTATGA CAGGACAGTT TAGCAACAAA 
GAACTTCAGG AAATGAAGGT ACAAGACAAT CAACTCTTCA GAGTTACCCC TCGTTCTGGT 
ACTTTGCTTC CAGGCCAGAA ATGTCCAGTA CACTTCTGCT ACAGTCATGA CTTTATTGGA 
ATTGACCGAC TTCCTGTTTT GTTCAAACTC AGTCTTGGCA GAGTTTTTTT GCTCAACTTT 
CAAGGAGTGA CATTGAACCG AAATGAACCA TATCTACACT TTGCTTCCAA TCACCATGTA 
TTCAAGCCTG TAGCTATTCG GGACTCCAGT CCTCCACGAC AGATATATGA GTTGTACAAC 
GGTGGTGCGG TGACAGTACA TTATGAAGTG GACCAAGTTG TATTGTCACA GCTTCAGATG 
GAGAACTATG ATCACCCAGT CTTGAGCTGT CTCAGTCCAC AAGGAGAAGT TCTCCCTGGG 
AATAAAGCCA TGCTCGAATG GATCTTCTCT CCATTGGAGG CTAAGATGTA CCATGTGGAT 
GTTCCCATCC ACATCCGGGA AAAGGATTCG GTAATTATAA ACTTTGAGGG ACGTGGGATG 
AATGCATTAT CAACAAGCGC CAATATCTCC ATTGAATACA GCGATGCTAA GACTCAAGAA 
CACCGTGCCC AGAAGGGGCC ATTCCCAGGA CAGGTGGCTT GGTTGTCCAA GGATAGTGTC 
TTTATTGGCG ACATCCCTGT TTGCACAAAG TCTTCGAGAA TCATGTTCAT CACCAGTTTG 
TCTTGCACAG ATACTGTGTA CTTTGTCTGG GAGATTCCTA CACAGAGCAA CCAACAAGTG 
GTACAGATCC ATCCAGAACG AGGCTGTCTT GGTCCAGGAG AGTGTGCTAT GTGCATCCTG 
ACCTTCGTTT CTGATTTCCC CACTGTTTAT CAGCTAGATC TCATATGTCA GGTCACTCAG 
AACGCTGCAC TCATCCATTA TCACAAAGCC TTGCAATGCT GGGAGGAAGA AAAGAAACGA 
CAGCAAAACG AATTCACAAT CACAGACAAG ATCCCTTCCC GCACTCAAAG AGTTCTAGTA 
GATGAAATAC TTCTCGCACC TCCTGCAAGA AAAGGGGCGC CCCTCCCAAA ATACAAGACT 
CTTCCTCCAA TCTGTGGCAG CAAAGCCGTC ATCAATTTAT ACGACAAAAA TAAGAGAGCT 
CAAATACGAC TACAGCGAGA ATCAGCTAAC ATATGGAGAA GACCCCAAAT GCCCCAACCT 
GCTCTGCTGC ACCTGAGTGT CACTGCCCAC ACCTATGACG TTCCGGACTT CTACACACAC 
TTTCCTGATC GATACCTTCA GTTGACCAGG ATCAAACAAA GATTAATCAA TTCTTCAAGC 
ACACGCCGCC TTGCGAAGCT GTGTCCATCT ACATGTGGCG CTGAGAGAGA CATCGTTGTG 
GACATACTCA CTCCTCTTTA CAAAGATCTC CTTGAAGATT CGGCTTTTGT AGAGTCTTTG 
ATGGTTTTAG CCTCCAAGCC TCCAATCTAC GAGGGTCAGA CCTCTACATC TCCCTCCGTC 
ACGTTTCCAT CTTCACCTCA ACATTCTGTT CAGTCGAACT GGACAGTGGA TGGAGAAGAA 
ACTGCAGTAT GTTTAGGGAA AATTCCTCAA ATCCAGGCCA CAGCGGAGTC CAAGCATGTT 
CCTTCTGACT TGGCTGAGGC TGTTTTGTTA AAGACACTCC AGAATTTAAT GATGGAAGCT 
GTGACTGGAG AACTGACCTT TTCAGCATAT TCCCGGAGCA AAGTACTGAC TTCGTTTTCA 
GTGAGCAGGA CGTCGTTTGC TGAAGGGAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023192274.1
CDS	114..5186
Translation	MNADYRSSKGHCCNEPQLPDIKQCQRKSHPQRSIFLGLETTLDLTWKDWVIEREYTKTLVISNIRNKLQKLHISAPETKFFSILNPEVVILSPGTSFSIPVTFKPLQKCGYKDCIQFQAIEGSFKVYLHAVLSSHTMKVPESVMLPLCAVEHYTLTSFPLKNISKVQMSFKWNSAAPFQLSPEQGRLKPGQQCVITVDFQPLEAQVYQKQVHCTYGEDGNKANSCCTVLLQGIGKYPFLQLRISNDKEETSHGDIELDFGSVAVGHSLSKQFEIFNPSPVSVWFSLSQLPGAVAIFGPHFSWDVTSEKIVPGGSVQVLVTYSPTMVDIVSVEYLSLNYRGSLSKSQVKLIGKCMGPELCLSSTVVDFGCVEEKGSAEQTVELLNSSPVQATYQWDIDCANSVFRVSPASGTVLPYGQIKVKVSYKPTHPMPHHRRVACLILLGEPLFLDLIGTCHTNLQEPVALTFEHLVEYKSKLCYMCNSSDTLLGVPLEEEGNSATAIPPAPMEKLNEIREEHVDSLFSPSFPFVSVEPSELLFNHKTRSFIAPSFAVSQCVTFKNHTKEKLSLMWTVIQDSPFCVTPSSFELAPLKSTTALVTYEPRQINALHGGELECFAYCKMPQEDPSSEQDLPCLPCCVTVRVISHSFQPGKDHFTPSCILKPHQVVFPPLSLISYRTVLLQNNGDQPLTFCLNYRTNSDLPRSVHMSPGCGFIQPGQHQILTIGAVPTLDSPTEGFNLPLQLNADEFTKELTVVSSLKKPCVSFENSNELHFTATAVGSKSQHSHSIKNLSSVPITFQWIISERDQNLICVEPDAGKLHPNESLVQTWTFSPLTEKAYTFKPTLSFQSDDFNKSQLIFRVNGTGAAGTIEAGKELLDMGESLVGSCRSFNIPIVNNCSCPISFHLSVQLEENPPDSEDNSALHLDVETGTIASHSTVLIGSTLRLDAQGWYRWAISYQITDGNGFGVCPPEKLCEVRAKGVFPTLQVVDACSSGSVDRLCKQYLWKLFSLDSFNEHLLSTPCPAEITFKAPTKHRQNSVSSFPQVILDFNFGAGPLHSDPSDFMLMFWNPGSISVNWTFLFPEDQEMKLEDWAMTGQFSNKELQEMKVQDNQLFRVTPRSGTLLPGQKCPVHFCYSHDFIGIDRLPVLFKLSLGRVFLLNFQGVTLNRNEPYLHFASNHHVFKPVAIRDSSPPRQIYELYNGGAVTVHYEVDQVVLSQLQMENYDHPVLSCLSPQGEVLPGNKAMLEWIFSPLEAKMYHVDVPIHIREKDSVIINFEGRGMNALSTSANISIEYSDAKTQEHRAQKGPFPGQVAWLSKDSVFIGDIPVCTKSSRIMFITSLSCTDTVYFVWEIPTQSNQQVVQIHPERGCLGPGECAMCILTFVSDFPTVYQLDLICQVTQNAALIHYHKALQCWEEEKKRQQNEFTITDKIPSRTQRVLVDEILLAPPARKGAPLPKYKTLPPICGSKAVINLYDKNKRAQIRLQRESANIWRRPQMPQPALLHLSVTAHTYDVPDFYTHFPDRYLQLTRIKQRLINSSSTRRLAKLCPSTCGAERDIVVDILTPLYKDLLEDSAFVESLMVLASKPPIYEGQTSTSPSVTFPSSPQHSVQSNWTVDGEETAVCLGKIPQIQATAESKHVPSDLAEAVLLKTLQNLMMEAVTGELTFSAYSRSKVLTSFSVSRTSFAEGS

Target ORF information:

RefSeq Version	XM_023336506.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus cilia and flagella associated protein 65 (cfap65), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023336506.1

ORF Insert Sequence:

ATGAATGCCG ATTACAGAAG TTCAAAAGGT CACTGCTGTA ATGAACCCCA ACTCCCTGAT 
ATAAAACAAT GTCAAAGAAA ATCCCATCCC CAAAGAAGCA TCTTCTTAGG CCTAGAAACC 
ACATTAGATC TGACATGGAA GGACTGGGTC ATTGAACGAG AGTACACCAA GACACTTGTG 
ATAAGCAACA TCCGTAATAA GCTGCAGAAG CTGCACATTA GTGCTCCAGA GACCAAGTTT 
TTCTCAATTT TGAATCCTGA AGTAGTTATT CTCAGCCCAG GGACTTCATT CTCCATACCG 
GTCACTTTTA AACCTCTCCA AAAGTGTGGG TATAAGGACT GCATTCAGTT TCAAGCTATT 
GAAGGAAGCT TTAAAGTATA CCTGCATGCT GTGCTTTCAA GTCATACAAT GAAGGTGCCT 
GAATCTGTGA TGCTGCCCCT ATGTGCTGTT GAGCACTACA CACTAACCAG TTTCCCACTA 
AAAAATATCA GTAAGGTTCA GATGTCATTT AAATGGAATA GTGCAGCTCC TTTCCAACTC 
AGTCCAGAAC AAGGCCGGCT AAAGCCCGGT CAGCAGTGTG TCATCACGGT GGACTTTCAG 
CCACTAGAGG CTCAGGTGTA CCAGAAGCAG GTGCACTGCA CATATGGAGA AGATGGCAAC 
AAAGCTAATA GCTGCTGCAC TGTGCTTTTA CAAGGGATAG GAAAATATCC ATTTCTTCAA 
TTAAGAATTT CCAATGACAA AGAGGAAACC AGCCATGGTG ATATTGAGTT GGACTTTGGG 
TCTGTGGCTG TTGGTCACAG TTTGAGTAAA CAATTTGAAA TCTTCAATCC TTCTCCTGTT 
AGTGTGTGGT TTTCTCTCTC ACAACTGCCT GGAGCGGTAG CCATCTTTGG ACCACACTTC 
AGTTGGGATG TCACAAGTGA GAAAATAGTG CCTGGTGGTT CGGTCCAGGT TTTGGTTACT 
TATTCTCCAA CTATGGTCGA CATAGTCTCT GTGGAGTACT TGTCTTTGAA TTATAGAGGG 
TCACTCAGTA AAAGTCAGGT CAAACTCATA GGAAAGTGTA TGGGTCCCGA ACTCTGTTTG 
TCCTCCACTG TGGTCGACTT TGGGTGTGTT GAAGAAAAAG GATCAGCAGA ACAAACAGTG 
GAACTGCTGA ACTCTTCCCC TGTTCAGGCT ACCTACCAAT GGGACATAGA CTGTGCAAAC 
AGTGTTTTTA GAGTTTCACC AGCAAGTGGC ACTGTGCTTC CATATGGTCA AATCAAAGTC 
AAGGTCTCCT ACAAACCCAC ACACCCTATG CCACACCACA GAAGAGTGGC ATGTCTTATA 
CTCCTCGGGG AGCCTCTGTT TCTGGACTTG ATTGGTACAT GCCACACAAA TCTTCAGGAG 
CCAGTAGCAT TAACATTTGA GCACTTGGTT GAGTACAAGT CTAAGTTGTG CTATATGTGT 
AACTCATCAG ACACACTGCT GGGGGTGCCT TTAGAAGAGG AAGGAAATAG TGCTACTGCC 
ATTCCTCCAG CACCTATGGA GAAATTGAAT GAAATCAGGG AAGAACACGT GGATTCTCTC 
TTCTCACCAT CATTTCCATT TGTATCTGTT GAACCAAGTG AACTGCTGTT TAATCACAAA 
ACAAGATCCT TTATCGCTCC ATCATTTGCT GTCTCACAGT GTGTTACCTT TAAAAATCAC 
ACCAAAGAGA AACTCAGCCT CATGTGGACC GTTATCCAAG ATTCCCCTTT CTGTGTTACT 
CCTTCGTCAT TTGAGTTGGC CCCACTGAAA TCTACCACTG CGCTTGTGAC ATATGAACCA 
AGACAGATCA ATGCCCTGCA TGGAGGAGAG CTTGAGTGCT TTGCATACTG TAAGATGCCA 
CAGGAGGATC CTTCCTCAGA ACAAGACCTT CCATGTCTGC CTTGTTGTGT GACTGTCAGA 
GTCATTAGCC ACTCTTTTCA GCCAGGGAAA GATCACTTCA CTCCTTCCTG TATTTTGAAG 
CCCCACCAAG TGGTTTTTCC ACCTCTGAGT CTTATTTCCT ATCGGACCGT GCTACTGCAG 
AACAATGGCG ATCAGCCTCT CACCTTCTGC CTGAACTACA GAACAAACAG TGACTTGCCA 
AGGTCTGTGC ATATGTCACC AGGCTGTGGT TTCATCCAGC CAGGACAACA CCAAATCCTT 
ACCATTGGAG CTGTCCCCAC TCTGGATAGT CCCACAGAGG GGTTTAATTT ACCATTGCAG 
CTTAATGCAG ATGAATTCAC GAAGGAACTG ACAGTTGTGA GCTCCTTAAA AAAGCCATGT 
GTGTCTTTTG AAAATAGCAA CGAATTACAT TTCACTGCTA CAGCTGTCGG CTCAAAATCA 
CAGCACAGCC ATTCCATCAA GAATTTAAGC AGCGTGCCCA TCACGTTTCA GTGGATCATT 
TCAGAGAGAG ATCAGAATCT TATCTGCGTT GAACCAGATG CTGGTAAACT GCATCCCAAT 
GAGAGCTTGG TCCAGACATG GACATTTTCT CCTCTGACAG AGAAAGCATA CACATTCAAA 
CCCACTCTCA GCTTCCAGAG CGATGACTTT AACAAGTCGC AACTCATTTT CAGAGTGAAT 
GGAACTGGTG CAGCTGGCAC CATTGAGGCA GGGAAAGAAC TCCTAGATAT GGGAGAGAGC 
TTAGTTGGAA GCTGCCGGTC GTTTAATATT CCTATAGTGA ACAACTGTAG CTGCCCCATC 
TCTTTTCATC TGTCTGTACA GCTAGAAGAA AATCCCCCTG ATTCTGAAGA TAACAGTGCT 
CTGCATCTGG ACGTTGAAAC GGGAACAATT GCTTCACATT CCACTGTGCT AATCGGTTCC 
ACTCTTAGAT TGGATGCACA AGGCTGGTAC CGCTGGGCCA TCAGCTACCA GATTACAGAT 
GGCAATGGCT TTGGTGTATG TCCTCCTGAG AAACTGTGTG AAGTGCGAGC TAAGGGGGTG 
TTCCCCACCT TACAGGTGGT TGATGCATGT AGCAGTGGCA GCGTGGACAG GCTCTGCAAG 
CAGTATTTGT GGAAACTCTT CTCATTAGAC AGTTTTAATG AGCACCTGCT TTCCACCCCA 
TGTCCTGCAG AAATTACTTT CAAAGCGCCC ACCAAGCACA GACAGAACAG TGTCTCCAGC 
TTCCCTCAAG TCATATTGGA TTTCAATTTT GGTGCTGGTC CTCTGCATTC AGATCCCTCA 
GATTTTATGT TGATGTTTTG GAACCCCGGT TCTATCTCTG TAAACTGGAC ATTCTTATTT 
CCAGAGGACC AAGAAATGAA GCTGGAGGAC TGGGCTATGA CAGGACAGTT TAGCAACAAA 
GAACTTCAGG AAATGAAGGT ACAAGACAAT CAACTCTTCA GAGTTACCCC TCGTTCTGGT 
ACTTTGCTTC CAGGCCAGAA ATGTCCAGTA CACTTCTGCT ACAGTCATGA CTTTATTGGA 
ATTGACCGAC TTCCTGTTTT GTTCAAACTC AGTCTTGGCA GAGTTTTTTT GCTCAACTTT 
CAAGGAGTGA CATTGAACCG AAATGAACCA TATCTACACT TTGCTTCCAA TCACCATGTA 
TTCAAGCCTG TAGCTATTCG GGACTCCAGT CCTCCACGAC AGATATATGA GTTGTACAAC 
GGTGGTGCGG TGACAGTACA TTATGAAGTG GACCAAGTTG TATTGTCACA GCTTCAGATG 
GAGAACTATG ATCACCCAGT CTTGAGCTGT CTCAGTCCAC AAGGAGAAGT TCTCCCTGGG 
AATAAAGCCA TGCTCGAATG GATCTTCTCT CCATTGGAGG CTAAGATGTA CCATGTGGAT 
GTTCCCATCC ACATCCGGGA AAAGGATTCG GTAATTATAA ACTTTGAGGG ACGTGGGATG 
AATGCATTAT CAACAAGCGC CAATATCTCC ATTGAATACA GCGATGCTAA GACTCAAGAA 
CACCGTGCCC AGAAGGGGCC ATTCCCAGGA CAGGTGGCTT GGTTGTCCAA GGATAGTGTC 
TTTATTGGCG ACATCCCTGT TTGCACAAAG TCTTCGAGAA TCATGTTCAT CACCAGTTTG 
TCTTGCACAG ATACTGTGTA CTTTGTCTGG GAGATTCCTA CACAGAGCAA CCAACAAGTG 
GTACAGATCC ATCCAGAACG AGGCTGTCTT GGTCCAGGAG AGTGTGCTAT GTGCATCCTG 
ACCTTCGTTT CTGATTTCCC CACTGTTTAT CAGCTAGATC TCATATGTCA GGTCACTCAG 
AACGCTGCAC TCATCCATTA TCACAAAGCC TTGCAATGCT GGGAGGAAGA AAAGAAACGA 
CAGCAAAACG AATTCACAAT CACAGACAAG ATCCCTTCCC GCACTCAAAG AGTTCTAGTA 
GATGAAATAC TTCTCGCACC TCCTGCAAGA AAAGGGGCGC CCCTCCCAAA ATACAAGACT 
CTTCCTCCAA TCTGTGGCAG CAAAGCCGTC ATCAATTTAT ACGACAAAAA TAAGAGAGCT 
CAAATACGAC TACAGCGAGA ATCAGCTAAC ATATGGAGAA GACCCCAAAT GCCCCAACCT 
GCTCTGCTGC ACCTGAGTGT CACTGCCCAC ACCTATGACG TTCCGGACTT CTACACACAC 
TTTCCTGATC GATACCTTCA GTTGACCAGG ATCAAACAAA GATTAATCAA TTCTTCAAGC 
ACACGCCGCC TTGCGAAGCT GTGTCCATCT ACATGTGGCG CTGAGAGAGA CATCGTTGTG 
GACATACTCA CTCCTCTTTA CAAAGATCTC CTTGAAGATT CGGCTTTTGT AGAGTCTTTG 
ATGGTTTTAG CCTCCAAGCC TCCAATCTAC GAGGGTCAGA CCTCTACATC TCCCTCCGTC 
ACGTTTCCAT CTTCACCTCA ACATTCTGTT CAGTCGAACT GGACAGTGGA TGGAGAAGAA 
ACTGCAGTAT GTTTAGGGAA AATTCCTCAA ATCCAGGCCA CAGCGGAGTC CAAGCATGTT 
CCTTCTGACT TGGCTGAGGC TGTTTTGTTA AAGACACTCC AGAATTTAAT GATGGAAGCT 
GTGACTGGAG AACTGACCTT TTCAGCATAT TCCCGGAGCA AAGTACTGAC TTCGTTTTCA 
GTGAGCAGGA CGTCGTTTGC TGAAGGGAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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cfap65 cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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