cenpf cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cenpf
Entrez Gene ID	102232107
Full Name	centromere protein F
Gene Type	protein-coding
Organism	Xiphophorus maculatus(southern platyfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023332347.1	XP_023188115.1	centromere protein F isoform X1
XM_023332348.1	XP_023188116.1	centromere protein F isoform X2
XM_005804243.1	XP_005804300.1	centromere protein F
XM_023332349.1	XP_023188117.1	centromere protein F isoform X3

Pathways from BioSystems
Gene Ontology

The following cenpf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cenpf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXi09585	XM_005804243.1 Latest version!	Xiphophorus maculatus centromere protein F, 350/400kDa (cenpf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXi37125	XM_023332347.1 Latest version!	Xiphophorus maculatus centromere protein F (cenpf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXi37126	XM_023332348.1 Latest version!	Xiphophorus maculatus centromere protein F (cenpf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXi37127	XM_023332349.1 Latest version!	Xiphophorus maculatus centromere protein F (cenpf), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cenpf ( XM_005804243.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_005804300.1 Xiphophorus maculatus centromere protein F, 350/400kDa (cenpf), mRNA.

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CloneID	OXi09585
Clone ID Related Accession (Same CDS sequence)	XM_005804243.1
Accession Version	XM_005804243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1445702400000
Organism	Xiphophorus maculatus(Southern platyfish)
Product	centromere protein F
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005372290.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xiphophorus maculatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 76% of CDS bases ##RefSeq-Attributes-END##

ATGAGCTGGG CGGTGGAGGA GTGGAAGGAT GGACTTCCAG GGAAAGCCCT GCAGAAGATC 
CAGGAGATGG AGGTCCAACT GGACAAACTG AAGAAGGAAA GGACACAGAA GCAGTTTCAG 
CTGGACTCTC TGGAAGCTGC CCTGCAGAAA CAGAAGCAGA AGGTGGACAG TGAACGCACC 
GAGATCTCCG CTTTGAAAAG AGAAAACCAG TCGATGATTG AGTCATGTGA CGCTTTGGAG 
AAAACTCGTC AGAAGGTGGT TCATGACCTT GGAGTCAAAG AGCAGCAGGT GAGCTACCTG 
GAGGGTCAAC TTAACTCCTG CAGGAAGACA ATAGATCGTC TGGAGCAAGA ACTCAAGAAG 
TACAAAACTG AGCTCGATCG CTCTCAACCT GCCGGATCTT CCTTGCTGTC CACCTCTTCC 
TCTGACCTGC AGACGTTCAG CACGCCACAG AAGACTTTTG CTACGCCAGC GCCTGTCGCT 
GCACACAGAC AGCCAGGTAT CCTGGCTTGC AGGTTCTTTG TGTTTCGTCT GGGCGGTCAT 
ATTTGCAGGT CTCACTCGGG TGTCTTTGCA GACAGCAAAC TGGACGAGCT TCAGGAAAAG 
TACAACCATG AAGTGGAAGA AAGAAGAAAG CTGGAAAGTG AGCTCAAAAT GTTGCAGGTC 
AAGGTGAGTT TTACTTTTCC TGATTCATCC GTCAGCCACA AGGACATCGC CGCCCGCCAA 
GCTGGGTCAT CCATATTCCC ATGGCAACAG CAGGATCAGG TCCACAGCCT ACGGGGCCAG 
GATGCGATGG AAACGCCACT AAAGAGACGC GGTGCTTCTC TGTGGGACAC CCACGAGGAA 
ACGCCGATCA AACCCAACCA GCGGATGAGT TCGTCCAGAA CCGTCCAGAG TCCCAGTGGA 
TCGACCCAGC AGACGGAGCA GCTGAAGAGC CTCAACCAGG AGCTGCGTGG ACGTGTGTCA 
GAGCTGGAGA GGAATCTGGC TGCTCAGGAA AAGGAAATTC GTAACCAAAC ATCGAAGCTG 
CAAGAACTCC AAACTCACTT GAACCAAACC CGCAAAGAGC TGGCGGAGCG GGACAGAGAG 
CTGGCCAAGG CCGGCCACGA GCTGAGCCAG GCTGCAGACC GCCACCAGCA GCTGGAGGCA 
AAGTGCTCCT TGGTGGAGCA GAAGCTGAAG CAGGTCTCCG AGGAGATGAG CTGCCAGAGG 
CACAACGCTG AAAGCTGCAG ACGAGCTCTG GAGCAGAAAC TCAAAGACCA AGAAAGAAAC 
AGTCAGAAGG AGTTGGCTCA GCTTCAGAGT TCCCATCAGG CTTTGGATCA GCAGCTCAAC 
CAGACCAGAA CCAAGCTGAC ACAGGAGATC CAACAGGCCA AAAAAGACCA CAATGTTCTT 
CAAGCGGACA TGGAGAAGAC GCGCTTGCAG AAGAGTCAGA TGGAGAGAGA AATGGAGGAG 
CAGAAGCAGA AGCTGCTGCG GTCCGAACAA GGCCTGCAGG CGACTCAGAC CAAAGAGCAA 
GACCTCCGCA AAAAGATGGA GGAGCTGCAG AAAGAGAAGA ACGGCGTGAC CATCCAGTTG 
GACCGCTGCA GCAGGTCGCT GACTCAGCTG GAAGAGGAGA AGAGGATCTC AGAGCAGTCG 
CTGAAACGCA CCCAGGGGCT GCTGGAGGAC CTGAAAGCGA AATCTGAAGG ACAGGCCGAG 
GAGCTGAAGA GACTCCAGTC AAAATTTGAG CAACAGACTC AGACGGCAGC AAGAGAGCTG 
GAACACGTGA AGAAGACGCT TTCTGACGCA GAGGCCAGGA ATGACAAATC TCAGAATGAG 
CTTCAAAAGC AGAAGCAGGA GAGTGAGAAG CTGAGCAACA AGCTGACGGT CATAGAGCAG 
GAGAGCGAAG AGCTGAAATC AAACCTCCGT CAGAGTCGGG ACGAGTGCCA GGCTGTGAAA 
CAGGAGCACC AGGCTCTGCT GGAGTGGAAG AAAAACAAGG AGAACTTGAT AAACCAAACC 
GAGGCCGCGC AGCAGGAGCT CACTGACAAG ATTATCACTT TGGAGGGCAA AGCGAGCCGG 
ATGCATGAGG CCAACAATGA ACTCCAGGAC AAGATCTCAT CCACGGAGGC AGACAAAGCC 
AGTCTGTCCG CCCACATCGA CGCCCTGAAG GGAGAACTCC TCATGAGGAG CACGGAGCTG 
GAGGAGAAGG AGCATCAGTA CCAGCAGCTT CAGCTCCACG TCTCTGAAGC GGCACAGAAA 
CACGGCAAAG ACCTGGAGAA CGCCGGGAAG CAGCTGGCGC AGCTCCAAGG ACAGGTGAAA 
GAGCTGGAGT CCCGGCTGCA GAGGGAGGCG TCTCGAGCAG AGCTGGCCGA AAAGACGAAC 
TGCGAGCTGC AGGAGGAGCA CCAGGCCGCG TGCGACCTGC TGCGCTCCAA AGACCAGCTG 
CTGGAGCTGG GCCGGGCCGA GGTCAGCCAG CTGAAGGACA GCCTCGCTCA GTCCGCCGCA 
CAGCAGGAGA TCACCTCGAT GGAGCAGATC CTGAAGGTCC AGGAACAGCT GGGAGCCGAG 
CTGCAGAAAC AAATTAAGAC GACGTCCGAT AAGGAGGAGG AGCTCACGAA GACGTGCGAG 
GAGAAATCAG AGGAGCTGAA ACGCTTGGAG GAGGAGCTGA AGGACCAGCG GAGCCTCACC 
GAGGAGACGG AGCAGCAGAT CAGAGCGGCT GAAGCTCGGA TCCACTCCGT GGAAAATCAA 
AAGGCTGAGC TGGAAAATCG ACTTCAAGAG ATGAAGACAG AAGCAGAGAT CCTGCAGTTC 
AGTCACACTG AAAGGATGAA CGCTCTCCAG GAACAGATCG TATGTCTGGA AAAGCAGGTT 
GCTGCAAACC AGGAAGAAGC AGAGGAGGTT CCTGTCCTGA AAAACAAACT GGACGTGGTC 
AACCAGTCTC TTGCTCACCT TAACAAATCT CTTGAAGCTT CAGAAAAGAG CCTCAGCTGT 
GCAAATGAAA TAAAAGCTGA TCTGGAGGTC ACCCTGTCTG AGAAAGTGAA ACTTATCACT 
GCTTTGGAGG ATCAGATCAA TAATCTCACT GAGAAGCTGA GGAAAGAGTC TGAGAGCCAC 
AGCTCAGAGG TTGAGGACTT CATCAATAAG GAGAGGAGCC TCAAAGAGCA GCTGGAAGCC 
ACTAGGAAAT CAGTAGCTGC AGCCAAAGAA GAGTCCAGCT CTAGACGGGA GAATATCCGA 
GAAATGAAGA TGACTCTGTC TGCAGCGTCC CGCGGCCTGG AAGAAAGAGA CAACACGATA 
AAGAGTCTGA AAGAGAAGCT GAACAAAGCA GAGGCAGAGC AGGCCAAAGC CTCTGAGCTC 
CTGAAGGAGA AGACGGTGGC CATGAGCAAA ATCAAGGTGC AGCTGGAGAT GCTGCAGATG 
GACCTGGAGG ACAACGAGTT GGCCATGACC TCTGTGGGCA GTCAGGCGGA GGAGCTGAAG 
GGAACCATAG CGTCCCTGGA GGCCATGCTG GAGGAAAGCA AAGCCCAGGT TTCTGTTTTG 
GAGACCCGTT TGGATGAGGT CCAGTACCAA AACTCTCTGC TGGAGACAAA ATACGTGACG 
GCGAAGGACG AGCTGCTGGA GCGGAGCTGC GAGATAACCC GTCTGGAGGA GGACGCCGTC 
AGACGGCAGC AGCTAGAGGA GAACGTCTCT GCCTTGGAGG CCAAACTGAG TCAGATTACT 
GAGGAAAACT CAAAGGCAGA GACGGAGCTG AGGCAGATCA TTCAGGAGAA AGAGAGCCAT 
TTGGAGCAAG TAAACGAGGA GAGAAACAGC CTCCAGGCTA CCTTAACGCA GTCAGTGGAT 
GAGCAGAAAC GAGCTGAATC TGAAATCACT CAGGTAAAAG CGGTGAAGGC GGAGCTGGAG 
GAGAGAATGA GCAACCTGTC GAAGGAGATT CAGCAACTCC ATGCTGATGT TAGAGAGTTA 
TCTGAACAGA AACAGGAAGC GGAGGGAACG ATTGACCAGA TGGCTGCAGA AACACAACAG 
ACAGAGTCCG CTCTCCGTCG GATCTCAGAG GAGAAAGCCC AGCTGGACGT TGCTCTGAGT 
TCAGTCAGCG AGGAAAAAGT TGAGCTGCAG GAGAGGATTT CTACTTTAAC CTCTGAGAAC 
GGGGAGCTGG TCTGCAAAGT GAAGCAGGTG GAGGAGGAGA AACTCCAGCT GGAGTCTAAG 
TTTAGTCAAA TCTCTGAGGA AAACGTCAAG CAGCATGCAG AGAGGCTGCA GGAGGAGATG 
GCGTCTCTGC GGGAGGAGAG CGAGCGTGTC CAGAGCTCGC TGCTGTCCGC TGAGGAGGAG 
CGACAGATGC TGCGGGAGCA GCTCAACACG AGGGACGAGA CCAACCACAC GGAGAGCAGA 
GCGCGCAACC AGCAGTTTGA GGCGGAGCAG ACTGCACTTC ATCAGAAGCT GTCCATCCTC 
CAGACGGAGC TGGCTGTGCT GCAGCAGCAG TACGATTTGC TTCTGCAGCA AGTGGACCAG 
CAGCAACGCA TCATTCAGCA GCTCACAGAA TCACAGAAAT ACCAGAATCC GACTGGAAAC 
ATCCCGCGCC TCGAACCCAA AGACGCTGAG GATGGCGAGG CAGCTGAACA GACCAAACCA 
AACCCAGAAC TTGGTGCGAA CGTCGGTACC AGTGCAGAGT CAGATCCACT GAATGAACAG 
GAAGAGGCGT CTGAAAAGAG CCCTGATCAG GCTGCGAGTG AGGCAGAGCT GATCGTTCGG 
GAGGACGCCT CTGATCAGGA GATGATCAAT GAGGAGGAGG CTCATAAACA TGATGCTCAG 
GAAATCCAAC TTCGCCAGCA GGTCTCTGAG GAGAACTGCA GCAGTAGTGG AGATCTTGAA 
AATGAGAAGT ATGATGCATC TTCCTTCCAA CATGAGATAA GAGGCATGAA ATTTTCTGGA 
GACGAGTCTT GGCCGGTGAA AGACGTCGAT ACAGAAATGA TCTCAGAGCA GCAGAGTGAA 
CTGAAGACGC TGCGGTCGGA GTTTGACCTG CTGACCTCTG AGCTGCAGCT GAGAGCAGAG 
CTGACCTCAG AGCTGGAAGT CCAAGTTCAG AAGTTGGAGG AGAAAGTTCA GGCTGCAGAG 
GAGCAGCTGA GCACCGCTTT GGAGGAGAAG AAGAGTCTTT CTGATCAGGT GACCCAGCTG 
CTGGAGGAGA GGGAGTCACT TAGTCTGCAG CTGGAAACGT CTAAATGTCA ACTCACTGAC 
TTCATGGAGA TGTTGGAAGG ACTTGAGATG GCCAAAGGCG GATTGGATGA GAAGTTCCTT 
CAGCAGGAGA GTGAGTTGAA GAGGGTCCGC TCTGAAAAAG CTAACCTGGA GCAGCACATC 
CTGGGAATGG AGTCTGAACT GGAGAGCATG CAAGCAGAAA CCTCCAGGCT TACGGAGGAG 
CTGGAGATCC AGAGGAAGAC TTGTTCAGGA CGGGAACAAC AGATAGAAAC TCTCCTTACG 
GAGACGACCC AGCTGAGAGC TGAACTCGTG TCGTGCTCTG AAGACCGAGA TGAGTTGAGT 
CAGTCTTTAG GTCAGTGGAG AGACAAAGTT CACGGTTTGG AGAAGACAAA CCTGGAAACC 
AGAAATTTAA TCTCCATCCT GGAGGAAGAC ATCCGGGTCG GAAGAAAGGA GTACGAAGGT 
CTGCAAAGCG GCATGGAGAG AGTGAAGGCA GAGAGGGAGC AGGTTGTGGT TCTGGAAGAG 
GCCATTTCTA AACATAACAG GGAGAAGGAG GGGCTGCTCA ACCACCTCCA TGAGATGGAA 
GAAGATCACA CGTCTACCAA CCAAAACACC GAGTCCATGG CCGGCAAAAT CCAGGCGTTG 
GAGGGAGAAG TGTGTCGCCT CTCCCAGTCT CTGGAGTCGT CGCTGCTGGA GAAAGGAGAA 
ATCGCGTCTC GGCTGAACTC CACTCAGGAT GAAGTCCGGC AGATGAGGAC GGGCATCGAG 
AAGCTGCAGG TCCGCATCGA GTCAGACGAG AGGAAGAAGA AGAAGATGGG AGAGCTGCTC 
AAAGCTGCTC AGAGGAAGTC TGACTCGCTG CAGGATCGCA TCGATGCGCT GGAGCGAGAG 
AAAGACGAAT TTGAGCAAAA TCTAGAGGAG GCCGTGTTGC AGGCTGAAGT AGCAAAAGCT 
GAGCTGGAAG AGGAGAGGAC TAAGGTGGAA GAAGAGAAGA AAGAGCTGAA TGAGAAACTG 
ACTGAGCTGT CCGCCGCTCT GGACACTCTG ACATCTCAGA AAGCTCATTT AGAGAGAGAG 
CTGGACATGA AAAATGTAGA GATAGAGGAG CTGAAGGCAG CTAAGGACAA ACTGGAACAG 
GGTTTGGAGA AAGCAGAGGT GGACAGAAGA GAAGAAGACA GACAGAGACA AAGACTAGAG 
GAGCTGGAGA AATGGACGAG AGAGGAAGCA GAGAGACAAA GTGTGCGAGT CGGAGACCTT 
CAGGGCCAGC TGACGGAGTT GGAAAGGGAA AACAACGACC TTAGGGCAAC GTTTGAGTCT 
TTAGGAGAAG AAATAAAGGA TCTTAAAACA CTTGCTCAAG CTAAAGAGGA AGAAATCCTC 
GCTTTGGAGA AGGACAAAGA GAATAAGGCA GAATCCATCT CATTAATCGT AGCAGAGAAG 
AAACGATTAG AGGAAAGAAA TGAACAGCTG GAAAAGGAGA GAGACGACCT TCAGTCTGCT 
CTGGTGTCTG TGAACCAAGA GAAAGCAAAG GCAGATCAGG AGAAAACAGA GTTGGATGAA 
GGGAGAGTAA AGCTTCAGTC CAAGATCTCG TTGGTAGAAA CGGAGAAACA GAACCTCCAA 
CAAACCGTCT CCTTGTTGGA GCAGGAAAAG CTGAGGTCAG AGGCTGAAAC TGAGGAGTTG 
CAGTCTTCAC TGTTGATGAT GGAAGAGGAG AAGAACAACC TGTCTTCAGC TCTTTCTCTG 
CTGGAAGAAG AGAAGCAGCA GGCAACAGAG GAGAAAGAGA GGCTCACACA GGAACATGAG 
GTTCTCCAGA AAACAGTCGC CTCCATGGAA GAAGAACTGG AGACACACAA ACGATCCATG 
ACCCAGCTCA GTGAGCAGGT TTCTGAACTA ACATCCAGTT TAGCTCGTCT GACAAAAGAG 
AGAGACTCTG CTCTGAGCAA AATGAACCTT TGGATGAAGA CCTGCAAACA GCTGGAGCAG 
GAGAAGCAGA ACATCCTAAA CAGCTCAGGA AGAAGCAGTG AGGAGTTTCA GGCTGAAGCG 
GCCCAGCTGA GGGCGCAGGC AGAGGTCAGG AAGAAAGAAG TCCAAGAACT GAAAACCGCC 
CTGGAGAAAA AAACGACAGA GGCTGAGGAA AGACGACAAG AATTGAAGCA AAAGGAGGAA 
GAGGCCGATG AGAGCATAGA GAAATACTGC AGCCTGCTGG TCAAAGTCCA CAAGCTGGAG 
GAGAGCAATG ACGCGCTGAA GACCCGACTG GAGCATCTCA CTGCCAGCCA GCCTGTGAAT 
GAAGCCAAGG TCCCTCCTGA AACCCGCCGG CGTTCAGTGA GGAAGTCTTC CTCCAAACAC 
CAGGAAGAAA AAATGAGTGA AAACACAGAG AATCTGGTTC CCACGACAAC ACCGAGCTCT 
CCACAGGGAG GGTCTCCGGG GAAACGGGGT CATAAAGAAA TCAGTGACAG AGACAGCGCC 
CAGGAGGCTC TGCACAACCT GACGAAGAAA CTAAAAGCCA ACACCGCGAC GCCCAGAGGA 
AGAGGAGAAC AGGATGACGA AGAGTTCAGA CCAGAGGGCC TGCCTGACCT GGTGCAGAAA 
GGGTTCGCTG ATATCCCACT GGGGGAGGCC AGTCCGTACA TCATCCGGAG GACCACCGGG 
AGGCGCTGCA GTCCTCGCCT GGCTGCAAGG CAGACTAAGC CTGACATCAA GGTTTTGGGG 
CCCGTCCATC TGCAGAGTCC CTCTGGTTCA TCATCGGACG GCTCCGACAG GACGCGCCAG 
CCTCTGAGTG GCGAACAGGA ACCTTCGCTG AATGTCCATC AGGAAGCAGA GCAGAGAGAG 
AAACAGGTTT TAGTGGAGCA AACAAAACAA GGAGAAAACT GTCATGTTCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005804300.1
CDS	1..7974
Translation	MSWAVEEWKDGLPGKALQKIQEMEVQLDKLKKERTQKQFQLDSLEAALQKQKQKVDSERTEISALKRENQSMIESCDALEKTRQKVVHDLGVKEQQVSYLEGQLNSCRKTIDRLEQELKKYKTELDRSQPAGSSLLSTSSSDLQTFSTPQKTFATPAPVAAHRQPGILACRFFVFRLGGHICRSHSGVFADSKLDELQEKYNHEVEERRKLESELKMLQVKVSFTFPDSSVSHKDIAARQAGSSIFPWQQQDQVHSLRGQDAMETPLKRRGASLWDTHEETPIKPNQRMSSSRTVQSPSGSTQQTEQLKSLNQELRGRVSELERNLAAQEKEIRNQTSKLQELQTHLNQTRKELAERDRELAKAGHELSQAADRHQQLEAKCSLVEQKLKQVSEEMSCQRHNAESCRRALEQKLKDQERNSQKELAQLQSSHQALDQQLNQTRTKLTQEIQQAKKDHNVLQADMEKTRLQKSQMEREMEEQKQKLLRSEQGLQATQTKEQDLRKKMEELQKEKNGVTIQLDRCSRSLTQLEEEKRISEQSLKRTQGLLEDLKAKSEGQAEELKRLQSKFEQQTQTAARELEHVKKTLSDAEARNDKSQNELQKQKQESEKLSNKLTVIEQESEELKSNLRQSRDECQAVKQEHQALLEWKKNKENLINQTEAAQQELTDKIITLEGKASRMHEANNELQDKISSTEADKASLSAHIDALKGELLMRSTELEEKEHQYQQLQLHVSEAAQKHGKDLENAGKQLAQLQGQVKELESRLQREASRAELAEKTNCELQEEHQAACDLLRSKDQLLELGRAEVSQLKDSLAQSAAQQEITSMEQILKVQEQLGAELQKQIKTTSDKEEELTKTCEEKSEELKRLEEELKDQRSLTEETEQQIRAAEARIHSVENQKAELENRLQEMKTEAEILQFSHTERMNALQEQIVCLEKQVAANQEEAEEVPVLKNKLDVVNQSLAHLNKSLEASEKSLSCANEIKADLEVTLSEKVKLITALEDQINNLTEKLRKESESHSSEVEDFINKERSLKEQLEATRKSVAAAKEESSSRRENIREMKMTLSAASRGLEERDNTIKSLKEKLNKAEAEQAKASELLKEKTVAMSKIKVQLEMLQMDLEDNELAMTSVGSQAEELKGTIASLEAMLEESKAQVSVLETRLDEVQYQNSLLETKYVTAKDELLERSCEITRLEEDAVRRQQLEENVSALEAKLSQITEENSKAETELRQIIQEKESHLEQVNEERNSLQATLTQSVDEQKRAESEITQVKAVKAELEERMSNLSKEIQQLHADVRELSEQKQEAEGTIDQMAAETQQTESALRRISEEKAQLDVALSSVSEEKVELQERISTLTSENGELVCKVKQVEEEKLQLESKFSQISEENVKQHAERLQEEMASLREESERVQSSLLSAEEERQMLREQLNTRDETNHTESRARNQQFEAEQTALHQKLSILQTELAVLQQQYDLLLQQVDQQQRIIQQLTESQKYQNPTGNIPRLEPKDAEDGEAAEQTKPNPELGANVGTSAESDPLNEQEEASEKSPDQAASEAELIVREDASDQEMINEEEAHKHDAQEIQLRQQVSEENCSSSGDLENEKYDASSFQHEIRGMKFSGDESWPVKDVDTEMISEQQSELKTLRSEFDLLTSELQLRAELTSELEVQVQKLEEKVQAAEEQLSTALEEKKSLSDQVTQLLEERESLSLQLETSKCQLTDFMEMLEGLEMAKGGLDEKFLQQESELKRVRSEKANLEQHILGMESELESMQAETSRLTEELEIQRKTCSGREQQIETLLTETTQLRAELVSCSEDRDELSQSLGQWRDKVHGLEKTNLETRNLISILEEDIRVGRKEYEGLQSGMERVKAEREQVVVLEEAISKHNREKEGLLNHLHEMEEDHTSTNQNTESMAGKIQALEGEVCRLSQSLESSLLEKGEIASRLNSTQDEVRQMRTGIEKLQVRIESDERKKKKMGELLKAAQRKSDSLQDRIDALEREKDEFEQNLEEAVLQAEVAKAELEEERTKVEEEKKELNEKLTELSAALDTLTSQKAHLERELDMKNVEIEELKAAKDKLEQGLEKAEVDRREEDRQRQRLEELEKWTREEAERQSVRVGDLQGQLTELERENNDLRATFESLGEEIKDLKTLAQAKEEEILALEKDKENKAESISLIVAEKKRLEERNEQLEKERDDLQSALVSVNQEKAKADQEKTELDEGRVKLQSKISLVETEKQNLQQTVSLLEQEKLRSEAETEELQSSLLMMEEEKNNLSSALSLLEEEKQQATEEKERLTQEHEVLQKTVASMEEELETHKRSMTQLSEQVSELTSSLARLTKERDSALSKMNLWMKTCKQLEQEKQNILNSSGRSSEEFQAEAAQLRAQAEVRKKEVQELKTALEKKTTEAEERRQELKQKEEEADESIEKYCSLLVKVHKLEESNDALKTRLEHLTASQPVNEAKVPPETRRRSVRKSSSKHQEEKMSENTENLVPTTTPSSPQGGSPGKRGHKEISDRDSAQEALHNLTKKLKANTATPRGRGEQDDEEFRPEGLPDLVQKGFADIPLGEASPYIIRRTTGRRCSPRLAARQTKPDIKVLGPVHLQSPSGSSSDGSDRTRQPLSGEQEPSLNVHQEAEQREKQVLVEQTKQGENCHVQ

Target ORF information:

RefSeq Version	XM_005804243.1
Organism	Xiphophorus maculatus(Southern platyfish)
Definition	Xiphophorus maculatus centromere protein F, 350/400kDa (cenpf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005804243.1

ORF Insert Sequence:

ATGAGCTGGG CGGTGGAGGA GTGGAAGGAT GGACTTCCAG GGAAAGCCCT GCAGAAGATC 
CAGGAGATGG AGGTCCAACT GGACAAACTG AAGAAGGAAA GGACACAGAA GCAGTTTCAG 
CTGGACTCTC TGGAAGCTGC CCTGCAGAAA CAGAAGCAGA AGGTGGACAG TGAACGCACC 
GAGATCTCCG CTTTGAAAAG AGAAAACCAG TCGATGATTG AGTCATGTGA CGCTTTGGAG 
AAAACTCGTC AGAAGGTGGT TCATGACCTT GGAGTCAAAG AGCAGCAGGT GAGCTACCTG 
GAGGGTCAAC TTAACTCCTG CAGGAAGACA ATAGATCGTC TGGAGCAAGA ACTCAAGAAG 
TACAAAACTG AGCTCGATCG CTCTCAACCT GCCGGATCTT CCTTGCTGTC CACCTCTTCC 
TCTGACCTGC AGACGTTCAG CACGCCACAG AAGACTTTTG CTACGCCAGC GCCTGTCGCT 
GCACACAGAC AGCCAGGTAT CCTGGCTTGC AGGTTCTTTG TGTTTCGTCT GGGCGGTCAT 
ATTTGCAGGT CTCACTCGGG TGTCTTTGCA GACAGCAAAC TGGACGAGCT TCAGGAAAAG 
TACAACCATG AAGTGGAAGA AAGAAGAAAG CTGGAAAGTG AGCTCAAAAT GTTGCAGGTC 
AAGGTGAGTT TTACTTTTCC TGATTCATCC GTCAGCCACA AGGACATCGC CGCCCGCCAA 
GCTGGGTCAT CCATATTCCC ATGGCAACAG CAGGATCAGG TCCACAGCCT ACGGGGCCAG 
GATGCGATGG AAACGCCACT AAAGAGACGC GGTGCTTCTC TGTGGGACAC CCACGAGGAA 
ACGCCGATCA AACCCAACCA GCGGATGAGT TCGTCCAGAA CCGTCCAGAG TCCCAGTGGA 
TCGACCCAGC AGACGGAGCA GCTGAAGAGC CTCAACCAGG AGCTGCGTGG ACGTGTGTCA 
GAGCTGGAGA GGAATCTGGC TGCTCAGGAA AAGGAAATTC GTAACCAAAC ATCGAAGCTG 
CAAGAACTCC AAACTCACTT GAACCAAACC CGCAAAGAGC TGGCGGAGCG GGACAGAGAG 
CTGGCCAAGG CCGGCCACGA GCTGAGCCAG GCTGCAGACC GCCACCAGCA GCTGGAGGCA 
AAGTGCTCCT TGGTGGAGCA GAAGCTGAAG CAGGTCTCCG AGGAGATGAG CTGCCAGAGG 
CACAACGCTG AAAGCTGCAG ACGAGCTCTG GAGCAGAAAC TCAAAGACCA AGAAAGAAAC 
AGTCAGAAGG AGTTGGCTCA GCTTCAGAGT TCCCATCAGG CTTTGGATCA GCAGCTCAAC 
CAGACCAGAA CCAAGCTGAC ACAGGAGATC CAACAGGCCA AAAAAGACCA CAATGTTCTT 
CAAGCGGACA TGGAGAAGAC GCGCTTGCAG AAGAGTCAGA TGGAGAGAGA AATGGAGGAG 
CAGAAGCAGA AGCTGCTGCG GTCCGAACAA GGCCTGCAGG CGACTCAGAC CAAAGAGCAA 
GACCTCCGCA AAAAGATGGA GGAGCTGCAG AAAGAGAAGA ACGGCGTGAC CATCCAGTTG 
GACCGCTGCA GCAGGTCGCT GACTCAGCTG GAAGAGGAGA AGAGGATCTC AGAGCAGTCG 
CTGAAACGCA CCCAGGGGCT GCTGGAGGAC CTGAAAGCGA AATCTGAAGG ACAGGCCGAG 
GAGCTGAAGA GACTCCAGTC AAAATTTGAG CAACAGACTC AGACGGCAGC AAGAGAGCTG 
GAACACGTGA AGAAGACGCT TTCTGACGCA GAGGCCAGGA ATGACAAATC TCAGAATGAG 
CTTCAAAAGC AGAAGCAGGA GAGTGAGAAG CTGAGCAACA AGCTGACGGT CATAGAGCAG 
GAGAGCGAAG AGCTGAAATC AAACCTCCGT CAGAGTCGGG ACGAGTGCCA GGCTGTGAAA 
CAGGAGCACC AGGCTCTGCT GGAGTGGAAG AAAAACAAGG AGAACTTGAT AAACCAAACC 
GAGGCCGCGC AGCAGGAGCT CACTGACAAG ATTATCACTT TGGAGGGCAA AGCGAGCCGG 
ATGCATGAGG CCAACAATGA ACTCCAGGAC AAGATCTCAT CCACGGAGGC AGACAAAGCC 
AGTCTGTCCG CCCACATCGA CGCCCTGAAG GGAGAACTCC TCATGAGGAG CACGGAGCTG 
GAGGAGAAGG AGCATCAGTA CCAGCAGCTT CAGCTCCACG TCTCTGAAGC GGCACAGAAA 
CACGGCAAAG ACCTGGAGAA CGCCGGGAAG CAGCTGGCGC AGCTCCAAGG ACAGGTGAAA 
GAGCTGGAGT CCCGGCTGCA GAGGGAGGCG TCTCGAGCAG AGCTGGCCGA AAAGACGAAC 
TGCGAGCTGC AGGAGGAGCA CCAGGCCGCG TGCGACCTGC TGCGCTCCAA AGACCAGCTG 
CTGGAGCTGG GCCGGGCCGA GGTCAGCCAG CTGAAGGACA GCCTCGCTCA GTCCGCCGCA 
CAGCAGGAGA TCACCTCGAT GGAGCAGATC CTGAAGGTCC AGGAACAGCT GGGAGCCGAG 
CTGCAGAAAC AAATTAAGAC GACGTCCGAT AAGGAGGAGG AGCTCACGAA GACGTGCGAG 
GAGAAATCAG AGGAGCTGAA ACGCTTGGAG GAGGAGCTGA AGGACCAGCG GAGCCTCACC 
GAGGAGACGG AGCAGCAGAT CAGAGCGGCT GAAGCTCGGA TCCACTCCGT GGAAAATCAA 
AAGGCTGAGC TGGAAAATCG ACTTCAAGAG ATGAAGACAG AAGCAGAGAT CCTGCAGTTC 
AGTCACACTG AAAGGATGAA CGCTCTCCAG GAACAGATCG TATGTCTGGA AAAGCAGGTT 
GCTGCAAACC AGGAAGAAGC AGAGGAGGTT CCTGTCCTGA AAAACAAACT GGACGTGGTC 
AACCAGTCTC TTGCTCACCT TAACAAATCT CTTGAAGCTT CAGAAAAGAG CCTCAGCTGT 
GCAAATGAAA TAAAAGCTGA TCTGGAGGTC ACCCTGTCTG AGAAAGTGAA ACTTATCACT 
GCTTTGGAGG ATCAGATCAA TAATCTCACT GAGAAGCTGA GGAAAGAGTC TGAGAGCCAC 
AGCTCAGAGG TTGAGGACTT CATCAATAAG GAGAGGAGCC TCAAAGAGCA GCTGGAAGCC 
ACTAGGAAAT CAGTAGCTGC AGCCAAAGAA GAGTCCAGCT CTAGACGGGA GAATATCCGA 
GAAATGAAGA TGACTCTGTC TGCAGCGTCC CGCGGCCTGG AAGAAAGAGA CAACACGATA 
AAGAGTCTGA AAGAGAAGCT GAACAAAGCA GAGGCAGAGC AGGCCAAAGC CTCTGAGCTC 
CTGAAGGAGA AGACGGTGGC CATGAGCAAA ATCAAGGTGC AGCTGGAGAT GCTGCAGATG 
GACCTGGAGG ACAACGAGTT GGCCATGACC TCTGTGGGCA GTCAGGCGGA GGAGCTGAAG 
GGAACCATAG CGTCCCTGGA GGCCATGCTG GAGGAAAGCA AAGCCCAGGT TTCTGTTTTG 
GAGACCCGTT TGGATGAGGT CCAGTACCAA AACTCTCTGC TGGAGACAAA ATACGTGACG 
GCGAAGGACG AGCTGCTGGA GCGGAGCTGC GAGATAACCC GTCTGGAGGA GGACGCCGTC 
AGACGGCAGC AGCTAGAGGA GAACGTCTCT GCCTTGGAGG CCAAACTGAG TCAGATTACT 
GAGGAAAACT CAAAGGCAGA GACGGAGCTG AGGCAGATCA TTCAGGAGAA AGAGAGCCAT 
TTGGAGCAAG TAAACGAGGA GAGAAACAGC CTCCAGGCTA CCTTAACGCA GTCAGTGGAT 
GAGCAGAAAC GAGCTGAATC TGAAATCACT CAGGTAAAAG CGGTGAAGGC GGAGCTGGAG 
GAGAGAATGA GCAACCTGTC GAAGGAGATT CAGCAACTCC ATGCTGATGT TAGAGAGTTA 
TCTGAACAGA AACAGGAAGC GGAGGGAACG ATTGACCAGA TGGCTGCAGA AACACAACAG 
ACAGAGTCCG CTCTCCGTCG GATCTCAGAG GAGAAAGCCC AGCTGGACGT TGCTCTGAGT 
TCAGTCAGCG AGGAAAAAGT TGAGCTGCAG GAGAGGATTT CTACTTTAAC CTCTGAGAAC 
GGGGAGCTGG TCTGCAAAGT GAAGCAGGTG GAGGAGGAGA AACTCCAGCT GGAGTCTAAG 
TTTAGTCAAA TCTCTGAGGA AAACGTCAAG CAGCATGCAG AGAGGCTGCA GGAGGAGATG 
GCGTCTCTGC GGGAGGAGAG CGAGCGTGTC CAGAGCTCGC TGCTGTCCGC TGAGGAGGAG 
CGACAGATGC TGCGGGAGCA GCTCAACACG AGGGACGAGA CCAACCACAC GGAGAGCAGA 
GCGCGCAACC AGCAGTTTGA GGCGGAGCAG ACTGCACTTC ATCAGAAGCT GTCCATCCTC 
CAGACGGAGC TGGCTGTGCT GCAGCAGCAG TACGATTTGC TTCTGCAGCA AGTGGACCAG 
CAGCAACGCA TCATTCAGCA GCTCACAGAA TCACAGAAAT ACCAGAATCC GACTGGAAAC 
ATCCCGCGCC TCGAACCCAA AGACGCTGAG GATGGCGAGG CAGCTGAACA GACCAAACCA 
AACCCAGAAC TTGGTGCGAA CGTCGGTACC AGTGCAGAGT CAGATCCACT GAATGAACAG 
GAAGAGGCGT CTGAAAAGAG CCCTGATCAG GCTGCGAGTG AGGCAGAGCT GATCGTTCGG 
GAGGACGCCT CTGATCAGGA GATGATCAAT GAGGAGGAGG CTCATAAACA TGATGCTCAG 
GAAATCCAAC TTCGCCAGCA GGTCTCTGAG GAGAACTGCA GCAGTAGTGG AGATCTTGAA 
AATGAGAAGT ATGATGCATC TTCCTTCCAA CATGAGATAA GAGGCATGAA ATTTTCTGGA 
GACGAGTCTT GGCCGGTGAA AGACGTCGAT ACAGAAATGA TCTCAGAGCA GCAGAGTGAA 
CTGAAGACGC TGCGGTCGGA GTTTGACCTG CTGACCTCTG AGCTGCAGCT GAGAGCAGAG 
CTGACCTCAG AGCTGGAAGT CCAAGTTCAG AAGTTGGAGG AGAAAGTTCA GGCTGCAGAG 
GAGCAGCTGA GCACCGCTTT GGAGGAGAAG AAGAGTCTTT CTGATCAGGT GACCCAGCTG 
CTGGAGGAGA GGGAGTCACT TAGTCTGCAG CTGGAAACGT CTAAATGTCA ACTCACTGAC 
TTCATGGAGA TGTTGGAAGG ACTTGAGATG GCCAAAGGCG GATTGGATGA GAAGTTCCTT 
CAGCAGGAGA GTGAGTTGAA GAGGGTCCGC TCTGAAAAAG CTAACCTGGA GCAGCACATC 
CTGGGAATGG AGTCTGAACT GGAGAGCATG CAAGCAGAAA CCTCCAGGCT TACGGAGGAG 
CTGGAGATCC AGAGGAAGAC TTGTTCAGGA CGGGAACAAC AGATAGAAAC TCTCCTTACG 
GAGACGACCC AGCTGAGAGC TGAACTCGTG TCGTGCTCTG AAGACCGAGA TGAGTTGAGT 
CAGTCTTTAG GTCAGTGGAG AGACAAAGTT CACGGTTTGG AGAAGACAAA CCTGGAAACC 
AGAAATTTAA TCTCCATCCT GGAGGAAGAC ATCCGGGTCG GAAGAAAGGA GTACGAAGGT 
CTGCAAAGCG GCATGGAGAG AGTGAAGGCA GAGAGGGAGC AGGTTGTGGT TCTGGAAGAG 
GCCATTTCTA AACATAACAG GGAGAAGGAG GGGCTGCTCA ACCACCTCCA TGAGATGGAA 
GAAGATCACA CGTCTACCAA CCAAAACACC GAGTCCATGG CCGGCAAAAT CCAGGCGTTG 
GAGGGAGAAG TGTGTCGCCT CTCCCAGTCT CTGGAGTCGT CGCTGCTGGA GAAAGGAGAA 
ATCGCGTCTC GGCTGAACTC CACTCAGGAT GAAGTCCGGC AGATGAGGAC GGGCATCGAG 
AAGCTGCAGG TCCGCATCGA GTCAGACGAG AGGAAGAAGA AGAAGATGGG AGAGCTGCTC 
AAAGCTGCTC AGAGGAAGTC TGACTCGCTG CAGGATCGCA TCGATGCGCT GGAGCGAGAG 
AAAGACGAAT TTGAGCAAAA TCTAGAGGAG GCCGTGTTGC AGGCTGAAGT AGCAAAAGCT 
GAGCTGGAAG AGGAGAGGAC TAAGGTGGAA GAAGAGAAGA AAGAGCTGAA TGAGAAACTG 
ACTGAGCTGT CCGCCGCTCT GGACACTCTG ACATCTCAGA AAGCTCATTT AGAGAGAGAG 
CTGGACATGA AAAATGTAGA GATAGAGGAG CTGAAGGCAG CTAAGGACAA ACTGGAACAG 
GGTTTGGAGA AAGCAGAGGT GGACAGAAGA GAAGAAGACA GACAGAGACA AAGACTAGAG 
GAGCTGGAGA AATGGACGAG AGAGGAAGCA GAGAGACAAA GTGTGCGAGT CGGAGACCTT 
CAGGGCCAGC TGACGGAGTT GGAAAGGGAA AACAACGACC TTAGGGCAAC GTTTGAGTCT 
TTAGGAGAAG AAATAAAGGA TCTTAAAACA CTTGCTCAAG CTAAAGAGGA AGAAATCCTC 
GCTTTGGAGA AGGACAAAGA GAATAAGGCA GAATCCATCT CATTAATCGT AGCAGAGAAG 
AAACGATTAG AGGAAAGAAA TGAACAGCTG GAAAAGGAGA GAGACGACCT TCAGTCTGCT 
CTGGTGTCTG TGAACCAAGA GAAAGCAAAG GCAGATCAGG AGAAAACAGA GTTGGATGAA 
GGGAGAGTAA AGCTTCAGTC CAAGATCTCG TTGGTAGAAA CGGAGAAACA GAACCTCCAA 
CAAACCGTCT CCTTGTTGGA GCAGGAAAAG CTGAGGTCAG AGGCTGAAAC TGAGGAGTTG 
CAGTCTTCAC TGTTGATGAT GGAAGAGGAG AAGAACAACC TGTCTTCAGC TCTTTCTCTG 
CTGGAAGAAG AGAAGCAGCA GGCAACAGAG GAGAAAGAGA GGCTCACACA GGAACATGAG 
GTTCTCCAGA AAACAGTCGC CTCCATGGAA GAAGAACTGG AGACACACAA ACGATCCATG 
ACCCAGCTCA GTGAGCAGGT TTCTGAACTA ACATCCAGTT TAGCTCGTCT GACAAAAGAG 
AGAGACTCTG CTCTGAGCAA AATGAACCTT TGGATGAAGA CCTGCAAACA GCTGGAGCAG 
GAGAAGCAGA ACATCCTAAA CAGCTCAGGA AGAAGCAGTG AGGAGTTTCA GGCTGAAGCG 
GCCCAGCTGA GGGCGCAGGC AGAGGTCAGG AAGAAAGAAG TCCAAGAACT GAAAACCGCC 
CTGGAGAAAA AAACGACAGA GGCTGAGGAA AGACGACAAG AATTGAAGCA AAAGGAGGAA 
GAGGCCGATG AGAGCATAGA GAAATACTGC AGCCTGCTGG TCAAAGTCCA CAAGCTGGAG 
GAGAGCAATG ACGCGCTGAA GACCCGACTG GAGCATCTCA CTGCCAGCCA GCCTGTGAAT 
GAAGCCAAGG TCCCTCCTGA AACCCGCCGG CGTTCAGTGA GGAAGTCTTC CTCCAAACAC 
CAGGAAGAAA AAATGAGTGA AAACACAGAG AATCTGGTTC CCACGACAAC ACCGAGCTCT 
CCACAGGGAG GGTCTCCGGG GAAACGGGGT CATAAAGAAA TCAGTGACAG AGACAGCGCC 
CAGGAGGCTC TGCACAACCT GACGAAGAAA CTAAAAGCCA ACACCGCGAC GCCCAGAGGA 
AGAGGAGAAC AGGATGACGA AGAGTTCAGA CCAGAGGGCC TGCCTGACCT GGTGCAGAAA 
GGGTTCGCTG ATATCCCACT GGGGGAGGCC AGTCCGTACA TCATCCGGAG GACCACCGGG 
AGGCGCTGCA GTCCTCGCCT GGCTGCAAGG CAGACTAAGC CTGACATCAA GGTTTTGGGG 
CCCGTCCATC TGCAGAGTCC CTCTGGTTCA TCATCGGACG GCTCCGACAG GACGCGCCAG 
CCTCTGAGTG GCGAACAGGA ACCTTCGCTG AATGTCCATC AGGAAGCAGA GCAGAGAGAG 
AAACAGGTTT TAGTGGAGCA AACAAAACAA GGAGAAAACT GTCATGTTCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cenpf ( XM_023332347.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023188115.1 Xiphophorus maculatus centromere protein F (cenpf), transcript variant X1, mRNA.

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CloneID	OXi37125
Clone ID Related Accession (Same CDS sequence)	XM_023332347.1
Accession Version	XM_023332347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	centromere protein F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036446.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG CGGTGGAGGA GTGGAAGGAT GGACTTCCAG GGAAAGCCCT GCAGAAGATC 
CAGGAGATGG AGGTCCAACT GGACAAACTG AAGAAGGAAA GGACACAGAA GCAGTTTCAG 
CTGGACTCTC TGGAAGCTGC CCTGCAGAAA CAGAAGCAGA AGGTGGACAG TGAACGCACC 
GAGATCTCCG CTTTGAAAAG AGAAAACCAG TCGATGATTG AGTCATGTGA CGCTTTGGAG 
AAAACTCGTC AGAAGGTGGT TCATGACCTT GGAGTCAAAG AGCAGCAGGT GAGCTACCTG 
GAGGGTCAAC TTAACTCCTG CAGGAAGACA ATAGATCGTC TGGAGCAAGA ACTCAAGAAG 
TACAAAACTG AGCTCGATCG CTCTCAACCT GCCGGATCTT CCTTGCTGTC CACCTCTTCC 
TCTGACCTGC AGACGTTCAG CACGCCACAG AAGACTTTTG CTACGCCAGC GCCTGTCGCT 
GCACACAGAC AGCCAGACAG CAAACTGGAC GAGCTTCAGG AAAAGTACAA CCATGAAGTG 
GAAGAAAGAA GAAAGCTGGA AAGTGAGCTC AAAATGTTGC AGGTCAAGTT GCTGAATCAG 
TCATCCGTCA GCCACAAGGA CATCGCCGCC CGCCAAGCTG GGTCATCCAT ATTCCCATGG 
CAACAGCAGG ATCAGGTCCA CAGCCTACGG GGCCAGGATG CGATGGAAAC GCCACTAAAG 
AGACGCGGTG CTTCTCTGTG GGACACCCAC GAGGAAACGC CGATCAAACC CAACCAGCGG 
ATGAGTTCGT CCAGAACCGT CCAGAGTCCC AGTGGATCGA CCCAGCAGAC GGAGCAGCTG 
AAGAGCCTCA ACCAGGAGCT GCGTGGACGT GTGTCAGAGC TGGAGAGGAA TCTGGCTGCT 
CAGGAAAAGG AAATTCGTAA CCAAACATCG AAGCTGCAAG AACTCCAAAC TCACTTGAAC 
CAAACCCGCA AAGAGCTGGC GGAGCGGGAC AGAGAGCTGG CCAAGGCCGG CCACGAGCTG 
AGCCAGGCTG CAGACCGCCA CCAGCAGCTG GAGGCAAAGT GCTCCTTGGT GGAGCAGAAG 
CTGAAGCAGG TCTCCGAGGA GATGAGCTGC CAGAGGCACA ACGCTGAAAG CTGCAGACGA 
GCTCTGGAGC AGAAACTCAA AGACCAAGAA AGAAACAGTC AGAAGGAGTT GGCTCAGCTT 
CAGAGTTCCC ATCAGGCTTT GGATCAGCAG CTCAACCAGA CCAGAACCAA GCTGACACAG 
GAGATCCAAC AGGCCAAAAA AGACCACAAT GTTCTTCAAG CGGACATGGA GAAGACGCGC 
TTGCAGAAGA GTCAGATGGA GAGAGAAATG GAGGAGCAGA AGCAGAAGCT GCTGCGGTCC 
GAACAAGGCC TGCAGGCGAC TCAGACCAAA GAGCAAGACC TCCGCAAAAA GATGGAGGAG 
CTGCAGAAAG AGAAGAACGG CGTGACCATC CAGTTGGACC GCTGCAGCAG GTCGCTGACT 
CAGCTGGAAG AGGAGAAGAG GATCTCAGAG CAGTCGCTGA AACGCACCCA GGGGCTGCTG 
GAGGACCTGA AAGCGAAATC TGAAGGACAG GCCGAGGAGC TGAAGAGACT CCAGTCAAAA 
TTTGAGCAAC AGACTCAGAC GGCAGCAAGA GAGCTGGAAC ACGTGAAGAA GACGCTTTCT 
GACGCAGAGG CCAGGAATGA CAAATCTCAG AATGAGCTTC AAAAGCAGAA GCAGGAGAGT 
GAGAAGCTGA GCAACAAGCT GACGGTCATA GAGCAGGAGA GCGAAGAGCT GAAATCAAAC 
CTCCGTCAGA GTCGGGACGA GTGCCAGGCT GTGAAACAGG AGCACCAGGC TCTGCTGGAG 
TGGAAGAAAA ACAAGGAGAA CTTGATAAAC CAAACCGAGG CCGCGCAGCA GGAGCTCACT 
GACAAGATTA TCACTTTGGA GGGCAAAGCG AGCCGGATGC ATGAGGCCAA CAATGAACTC 
CAGGACAAGA TCTCATCCAC GGAGGCAGAC AAAGCCAGTC TGTCCGCCCA CATCGACGCC 
CTGAAGGGAG AACTCCTCAT GAGGAGCACG GAGCTGGAGG AGAAGGAGCA TCAGTACCAG 
CAGCTTCAGC TCCACGTCTC TGAAGCGGCA CAGAAACACG GCAAAGACCT GGAGAACGCC 
GGGAAGCAGC TGGCGCAGCT CCAAGGACAG GTGAAAGAGC TGGAGTCCCG GCTGCAGAGG 
GAGGCGTCTC GAGCAGAGCT GGCCGAAAAG ACGAACTGCG AGCTGCAGGA GGAGCACCAG 
GCCGCGTGCG ACCTGCTGCG CTCCAAAGAC CAGCTGCTGG AGCTGGGCCG GGCCGAGGTC 
AGCCAGCTGA AGGACAGCCT CGCTCAGTCC GCCGCACAGC AGGAGGCGCA GAGCGACAGC 
TTTGTGAAGC AGAAGGCCGT CCTGCTGAAG CGGTGTGAGG AGAGCATTTC GGCGCAGGCT 
GAGGAGACGG AGCGCGTCAA GCTGCAGGCA GAGGAAGTCC AGCAGGAGCT GCTGCTCTCC 
AGACACAAAA TCACCTCGAT GGAGCAGATC CTGAAGGTCC AGGAACAGCT GGGAGCCGAG 
CTGCAGAAAC AAATTAAGAC GACGTCCGAT AAGGAGGAGG AGCTCACGAA GACGTGCGAG 
GAGAAATCAG AGGAGCTGAA ACGCTTGGAG GAGGAGCTGA AGGACCAGCG GAGCCTCACC 
GAGGAGACGG AGCAGCAGAT CAGAGCGGCT GAAGCTCGGA TCCACTCCGT GGAAAATCAA 
AAGGCTGAGC TGGAAAATCG ACTTCAAGAG ATGAAGACAG AAGCAGAGAT CCTGCAGTTC 
AGTCACACTG AAAGGATGAA CGCTCTCCAG GAACAGATCG TATGTCTGGA AAAGCAGGTT 
GCTGCAAACC AGGAAGAAGC AGAGGAGGTT CCTGTCCTGA AAAACAAACT GGACGTGGTC 
AACCAGTCTC TTGCTCACCT TAACAAATCT CTTGAAGCTT CAGAAAAGAG CCTCAGCTGT 
GCAAATGAAA TAAAAGCTGA TCTGGAGGTC ACCCTGTCTG AGAAAGTGAA ACTTATCACT 
GCTTTGGAGG ATCAGATCAA TAATCTCACT GAGAAGCTGA GGAAAGAGTC TGAGAGCCAC 
AGCTCAGAGG TTGAGGACTT CATCAATAAG GAGAGGAGCC TCAAAGAGCA GCTGGAAGCC 
ACTAGGAAAT CAGTAGCTGC AGCCAAAGAA GAGTCCAGCT CTAGACGGGA GAATATCCGA 
GAAATGAAGA TGACTCTGTC TGCAGCGTCC CGCGGCCTGG AAGAAAGAGA CAACACGATA 
AAGAGTCTGA AAGAGAAGCT GAACAAAGCA GAGGCAGAGC AGGCCAAAGC CTCTGAGCTC 
CTGAAGGAGA AGACGGTGGC CATGAGCAAA ATCAAGGTGC AGCTGGAGAT GCTGCAGATG 
GACCTGGAGG ACAACGAGTT GGCCATGACC TCTGTGGGCA GTCAGGCGGA GGAGCTGAAG 
GGAACCATAG CGTCCCTGGA GGCCATGCTG GAGGAAAGCA AAGCCCAGGT TTCTGTTTTG 
GAGACCCGTT TGGATGAGGT CCAGTACCAA AACTCTCTGC TGGAGACAAA ATACGTGACG 
GCGAAGGACG AGCTGCTGGA GCGGAGCTGC GAGATAACCC GTCTGGAGGA GGACGCCGTC 
AGACGGCAGC AGCTAGAGGA GAACGTCTCT GCCTTGGAGG CCAAACTGAG TCAGATTACT 
GAGGAAAACT CAAAGGCAGA GACGGAGCTG AGGCAGATCA TTCAGGAGAA AGAGAGCCAT 
TTGGAGCAAG TAAACGAGGA GAGAAACAGC CTCCAGGCTA CCTTAACGCA GTCAGTGGAT 
GAGCAGAAAC GAGCTGAATC TGAAATCACT CAGGTAAAAG CGGTGAAGGC GGAGCTGGAG 
GAGAGAATGA GCAACCTGTC GAAGGAGATT CAGCAACTCC ATGCTGATGT TAGAGAGTTA 
TCTGAACAGA AACAGGAAGC GGAGGGAACG ATTGACCAGA TGGCTGCAGA AACACAACAG 
ACAGAGTCCG CTCTCCGTCG GATCTCAGAG GAGAAAGCCC AGCTGGACGT TGCTCTGAGT 
TCAGTCAGCG AGGAAAAAGT TGAGCTGCAG GAGAGGATTT CTACTTTAAC CTCTGAGAAC 
GGGGAGCTGG TCTGCAAAGT GAAGCAGGTG GAGGAGGAGA AACTCCAGCT GGAGTCTAAG 
TTTAGTCAAA TCTCTGAGGA AAACGTCAAG CAGCAGTCAG ACATGAACCG TCTGACTGCA 
GAGAAGCAGC AGCTGCAGAG CAGTGCAGAG AGGCTGCAGG AGGAGATGGC GTCTCTGCGG 
GAGGAGAGCG AGCGTGTCCA GAGCTCGCTG CTGTCCGCTG AGGAGGAGCG ACAGATGCTG 
CGGGAGCAGC TCAACACGAG GGACGAGACC AACCACACGG AGAGCAGAGC GCGCAACCAG 
CAGTTTGAGG CGGAGCAGAC TGCACTTCAT CAGAAGCTGT CCATCCTCCA GACGGAGCTG 
GCTGTGCTGC AGCAGCAGTA CGATTTGCTT CTGCAGCAAG TGGACCAGCA GCAACGCATC 
ATTCAGCAGC TCACAGAATC ACAGAAATAC CAGAATCCGA CTGGAAACAT CCCGCGCCTC 
GAACCCAAAG ACGCTGAGGA TGGCGCAGAG GCAGCTGAAC AGACCAAACC AAACCCAGAA 
CTTGGTGCGA ACGTCGGTAC CAGTGCAGAG TCAGATCCAC TGAATGAACA GGAAGAGGCG 
TCTGAAAAGA GCCCTGATCA GGCTGCGAGT GAGGCAGAGC TGATCGTTCG GGAGGACGCC 
TCTGATCAGG AGATGATCAA TGAGGAGGAG GCTCATAAAC ATGATGCTCA GGAAATCCAA 
CTTCGCCAGC AGGTCTCTGA GGAGAACTGC AGCAGTAGTG GAGATCTTGA AAATGAGAAG 
TATGATGCAT CTTCCTTCCA ACATGAGATA AGAGGCATGA AATTTTCTGG AGACGAGTCT 
TGGCCGGTGA AAGACGTCGA TACAGAAATG ATCTCAGAGC AGCAGAGTGA ACTGAAGACG 
CTGCGGTCGG AGTTTGACCT GCTGACCTCT GAGCTGCAGC TGAGAGCAGA GCTGACCTCA 
GAGCTGGAAG TCCAAGTTCA GAAGTTGGAG GAGAAAGTTC AGGCTGCAGA GGAGCAGCTG 
AGCACCGCTT TGGAGGAGAA GAAGAGTCTT TCTGATCAGG TGACCCAGCT GCTGGAGGAG 
AGGGAGTCAC TTAGTCTGCA GCTGGAAACG TCTAAATGTC AACTCACTGA CTTCATGGAG 
ATGTTGGAAG GACTTGAGAT GGCCAAAGGC GGATTGGATG AGAAGTTCCT TCAGCAGGAG 
AGTGAGTTGA AGAGGGTCCG CTCTGAAAAA GCTAACCTGG AGCAGCACAT CCTGGGAATG 
GAGTCTGAAC TGGAGAGCAT GCAAGCAGAA ACCTCCAGGC TTACGGAGGA GCTGGAGATC 
CAGAGGAAGA CTTGTTCAGG ACGGGAACAA CAGATAGAAA CTCTCCTTAC GGAGACGACC 
CAGCTGAGAG CTGAACTCGT GTCGTGCTCT GAAGACCGAG ATGAGTTGAG TCAGTCTTTA 
GGTCAGTGGA GAGACAAAGT TCACGGTTTG GAGAAGACAA ACCTGGAAAC CAGAAATTTA 
ATCTCCATCC TGGAGGAAGA CATCCGGGTC GGAAGAAAGG AGTACGAAGG TCTGCAAAGC 
GGCATGGAGA GAGTGAAGGC AGAGAGGGAG CAGTTTTTGC AGCAGGTTGT GGTTCTGGAA 
GAGGCCATTT CTAAACATAA CAGGGAGAAG GAGGGGCTGC TCAACCACCT CCATGAGATG 
GAAGAAGATC ACACGTCTAC CAACCAAAAC ACCGAGTCCA TGGCCGGCAA AATCCAGGCG 
TTGGAGGGAG AAGTGTGTCG CCTCTCCCAG TCTCTGGAGT CGTCGCTGCT GGAGAAAGGA 
GAAATCGCGT CTCGGCTGAA CTCCACTCAG GATGAAGTCC GGCAGATGAG GACGGGCATC 
GAGAAGCTGC AGGTCCGCAT CGAGTCAGAC GAGAGGAAGA AGAAGAAGAT GGGAGAGCTG 
CTCAAAGCTG CTCAGAGGAA GTCTGACTCG CTGCAGGATC GCATCGATGC GCTGGAGCGA 
GAGAAAGACG AATTTGAGCA AAATCTAGAG GAGGCCGTGT TGCAGGCTGA AGTAGCAAAA 
GCTGAGCTGG AAGAGGAGAG GACTAAGGTG GAAGAAGAGA AGAAAGAGCT GAATGAGAAA 
CTGACTGAGC TGTCCGCCGC TCTGGACACT CTGACATCTC AGAAAGCTCA TTTAGAGAGA 
GAGCTGGACA TGAAAAATGT AGAGATAGAG GAGCTGAAGG CAGCTAAGGA CAAACTGGAA 
CAGGGTTTGG AGAAAGCAGA GGTGGACAGA AGAGAAGAAG ACAGACAGAG ACAAAGACTA 
GAGGAGCTGG AGAAATGGAC GAGAGAGGAA GCAGAGAGAC AAAGTGTGCG AGTCGGAGAC 
CTTCAGGGCC AGCTGACGGA GTTGGAAAGG GAAAACAACG ACCTTAGGGC AACGTTTGAG 
TCTTTAGGAG AAGAAATAAA GGATCTTAAA ACACTTGCTC AAGCTAAAGA GGAAGAAATC 
CTCGCTTTGG AGAAGGACAA AGAGAATAAG GCAGAATCCA TCTCATTAAT CGTAGCAGAG 
AAGAAACGAT TAGAGGAAAG AAATGAACAG CTGGAAAAGG AGAGAGACGA CCTTCAGTCT 
GCTCTGGTGT CTGTGAACCA AGAGAAAGCA AAGGCAGATC AGGAGAAAAC AGAGTTGGAT 
GAAGGGAGAG TAAAGCTTCA GTCCAAGATC TCGTTGGTAG AAACGGAGAA ACAGAACCTC 
CAACAAACCG TCTCCTTGTT GGAGCAGGAA AAGCTGAGGT CAGAGGCTGA AACTGAGGAG 
TTGCAGTCTT CACTGTTGAT GATGGAAGAG GAGAAGAACA ACCTGTCTTC AGCTCTTTCT 
CTGCTGGAAG AAGAGAAGCA GCAGGCAACA GAGGAGAAAG AGAGGCTCAC ACAGGAACAT 
GAGGTTCTCC AGAAAACAGT CGCCTCCATG GAAGAAGAAC TGGAGACACA CAAACGATCC 
ATGACCCAGC TCAGTGAGCA GGTTTCTGAA CTAACATCCA GTTTAGCTCG TCTGACAAAA 
GAGAGAGACT CTGCTCTGAG CAAAATGAAC CTTTGGATGA AGACCTGCAA ACAGCTGGAG 
CAGGAGAAGC AGAACATCCT AAACAGCTCA GAAGGAAGAA GCAGTGAGGA GTTTCAGGCT 
GAAGCGGCCC AGCTGAGGGC GCAGGCAGAG GTCAGGAAGA AAGAAGTCCA AGAACTGAAA 
ACCGCCCTGG AGAAAAAAAC GACAGAGGCT GAGGAAAGAC GACAAGAATT GAAGCAAAAG 
GAGGAAGAGG TGAAGGAGCG AGCGGCTGCT CTGGAGGGAG TGAGAGCGGA GAAGGAAAGG 
GAGCTGGAGG AGATCAGGAA GGAACTGGAT GAGGTTAACG CGCTTCTGGA GGAGAAAAGC 
ACCGAGGCCG ATGAGAGCAT AGAGAAATAC TGCAGCCTGC TGGTCAAAGT CCACAAGCTG 
GAGGAGAGCA ATGACGCGCT GAAGACCCGA CTGGAGCATC TCACTGCCAG CCAGCCTGTG 
AATGAAGCCA AGGTCCCTCC TGAAACCCGC CGGCGTTCAG TGAGGAAGTC TTCCTCCAAA 
CACCAGGAAG AAAAAATGAG TGAAAACACA GAGAATCTGG TTCCCACGAC AACACCGAGC 
TCTCCACAGG GAGGGTCTCC GGGGAAACGG GGTCATAAAG AAATCAGTGA CAGAGACAGC 
GCCCAGGAGG CTCTGCACAA CCTGACGAAG AAACTAAAAG CCAACACCGC GACGCCCAGA 
GGAAGAGGAG AACAGGATGA CGAAGAGTTC AGACCAGAGG GCCTGCCTGA CCTGGTGCAG 
AAAGGGTTCG CTGATATCCC ACTGGGGGAG GCCAGTCCGT ACATCATCCG GAGGACCACC 
GGGAGGCGCT GCAGTCCTCG CCTGGCTGCA AGGCAGACTA AGCCTGACAT CAAGGTTTTG 
GGGCCCGTCC ATCTGCAGAG TCCCTCTGGT TCATCATCGG ACGGCTCCGA CAGGACGCGC 
CAGCCTCTGA GTGGCGAACA GGAACCTTCG CTGAATGTCC ATCAGGAAGC AGAGCAGAGA 
GAGAAACAGG TTTTAGTGGA GCAAACAAAA CAAGGAGAAA ACTGTCATGT TCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023188115.1
CDS	53..8269
Translation	MSWAVEEWKDGLPGKALQKIQEMEVQLDKLKKERTQKQFQLDSLEAALQKQKQKVDSERTEISALKRENQSMIESCDALEKTRQKVVHDLGVKEQQVSYLEGQLNSCRKTIDRLEQELKKYKTELDRSQPAGSSLLSTSSSDLQTFSTPQKTFATPAPVAAHRQPDSKLDELQEKYNHEVEERRKLESELKMLQVKLLNQSSVSHKDIAARQAGSSIFPWQQQDQVHSLRGQDAMETPLKRRGASLWDTHEETPIKPNQRMSSSRTVQSPSGSTQQTEQLKSLNQELRGRVSELERNLAAQEKEIRNQTSKLQELQTHLNQTRKELAERDRELAKAGHELSQAADRHQQLEAKCSLVEQKLKQVSEEMSCQRHNAESCRRALEQKLKDQERNSQKELAQLQSSHQALDQQLNQTRTKLTQEIQQAKKDHNVLQADMEKTRLQKSQMEREMEEQKQKLLRSEQGLQATQTKEQDLRKKMEELQKEKNGVTIQLDRCSRSLTQLEEEKRISEQSLKRTQGLLEDLKAKSEGQAEELKRLQSKFEQQTQTAARELEHVKKTLSDAEARNDKSQNELQKQKQESEKLSNKLTVIEQESEELKSNLRQSRDECQAVKQEHQALLEWKKNKENLINQTEAAQQELTDKIITLEGKASRMHEANNELQDKISSTEADKASLSAHIDALKGELLMRSTELEEKEHQYQQLQLHVSEAAQKHGKDLENAGKQLAQLQGQVKELESRLQREASRAELAEKTNCELQEEHQAACDLLRSKDQLLELGRAEVSQLKDSLAQSAAQQEAQSDSFVKQKAVLLKRCEESISAQAEETERVKLQAEEVQQELLLSRHKITSMEQILKVQEQLGAELQKQIKTTSDKEEELTKTCEEKSEELKRLEEELKDQRSLTEETEQQIRAAEARIHSVENQKAELENRLQEMKTEAEILQFSHTERMNALQEQIVCLEKQVAANQEEAEEVPVLKNKLDVVNQSLAHLNKSLEASEKSLSCANEIKADLEVTLSEKVKLITALEDQINNLTEKLRKESESHSSEVEDFINKERSLKEQLEATRKSVAAAKEESSSRRENIREMKMTLSAASRGLEERDNTIKSLKEKLNKAEAEQAKASELLKEKTVAMSKIKVQLEMLQMDLEDNELAMTSVGSQAEELKGTIASLEAMLEESKAQVSVLETRLDEVQYQNSLLETKYVTAKDELLERSCEITRLEEDAVRRQQLEENVSALEAKLSQITEENSKAETELRQIIQEKESHLEQVNEERNSLQATLTQSVDEQKRAESEITQVKAVKAELEERMSNLSKEIQQLHADVRELSEQKQEAEGTIDQMAAETQQTESALRRISEEKAQLDVALSSVSEEKVELQERISTLTSENGELVCKVKQVEEEKLQLESKFSQISEENVKQQSDMNRLTAEKQQLQSSAERLQEEMASLREESERVQSSLLSAEEERQMLREQLNTRDETNHTESRARNQQFEAEQTALHQKLSILQTELAVLQQQYDLLLQQVDQQQRIIQQLTESQKYQNPTGNIPRLEPKDAEDGAEAAEQTKPNPELGANVGTSAESDPLNEQEEASEKSPDQAASEAELIVREDASDQEMINEEEAHKHDAQEIQLRQQVSEENCSSSGDLENEKYDASSFQHEIRGMKFSGDESWPVKDVDTEMISEQQSELKTLRSEFDLLTSELQLRAELTSELEVQVQKLEEKVQAAEEQLSTALEEKKSLSDQVTQLLEERESLSLQLETSKCQLTDFMEMLEGLEMAKGGLDEKFLQQESELKRVRSEKANLEQHILGMESELESMQAETSRLTEELEIQRKTCSGREQQIETLLTETTQLRAELVSCSEDRDELSQSLGQWRDKVHGLEKTNLETRNLISILEEDIRVGRKEYEGLQSGMERVKAEREQFLQQVVVLEEAISKHNREKEGLLNHLHEMEEDHTSTNQNTESMAGKIQALEGEVCRLSQSLESSLLEKGEIASRLNSTQDEVRQMRTGIEKLQVRIESDERKKKKMGELLKAAQRKSDSLQDRIDALEREKDEFEQNLEEAVLQAEVAKAELEEERTKVEEEKKELNEKLTELSAALDTLTSQKAHLERELDMKNVEIEELKAAKDKLEQGLEKAEVDRREEDRQRQRLEELEKWTREEAERQSVRVGDLQGQLTELERENNDLRATFESLGEEIKDLKTLAQAKEEEILALEKDKENKAESISLIVAEKKRLEERNEQLEKERDDLQSALVSVNQEKAKADQEKTELDEGRVKLQSKISLVETEKQNLQQTVSLLEQEKLRSEAETEELQSSLLMMEEEKNNLSSALSLLEEEKQQATEEKERLTQEHEVLQKTVASMEEELETHKRSMTQLSEQVSELTSSLARLTKERDSALSKMNLWMKTCKQLEQEKQNILNSSEGRSSEEFQAEAAQLRAQAEVRKKEVQELKTALEKKTTEAEERRQELKQKEEEVKERAAALEGVRAEKERELEEIRKELDEVNALLEEKSTEADESIEKYCSLLVKVHKLEESNDALKTRLEHLTASQPVNEAKVPPETRRRSVRKSSSKHQEEKMSENTENLVPTTTPSSPQGGSPGKRGHKEISDRDSAQEALHNLTKKLKANTATPRGRGEQDDEEFRPEGLPDLVQKGFADIPLGEASPYIIRRTTGRRCSPRLAARQTKPDIKVLGPVHLQSPSGSSSDGSDRTRQPLSGEQEPSLNVHQEAEQREKQVLVEQTKQGENCHVQ

Target ORF information:

RefSeq Version	XM_023332347.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus centromere protein F (cenpf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023332347.1

ORF Insert Sequence:

ATGAGCTGGG CGGTGGAGGA GTGGAAGGAT GGACTTCCAG GGAAAGCCCT GCAGAAGATC 
CAGGAGATGG AGGTCCAACT GGACAAACTG AAGAAGGAAA GGACACAGAA GCAGTTTCAG 
CTGGACTCTC TGGAAGCTGC CCTGCAGAAA CAGAAGCAGA AGGTGGACAG TGAACGCACC 
GAGATCTCCG CTTTGAAAAG AGAAAACCAG TCGATGATTG AGTCATGTGA CGCTTTGGAG 
AAAACTCGTC AGAAGGTGGT TCATGACCTT GGAGTCAAAG AGCAGCAGGT GAGCTACCTG 
GAGGGTCAAC TTAACTCCTG CAGGAAGACA ATAGATCGTC TGGAGCAAGA ACTCAAGAAG 
TACAAAACTG AGCTCGATCG CTCTCAACCT GCCGGATCTT CCTTGCTGTC CACCTCTTCC 
TCTGACCTGC AGACGTTCAG CACGCCACAG AAGACTTTTG CTACGCCAGC GCCTGTCGCT 
GCACACAGAC AGCCAGACAG CAAACTGGAC GAGCTTCAGG AAAAGTACAA CCATGAAGTG 
GAAGAAAGAA GAAAGCTGGA AAGTGAGCTC AAAATGTTGC AGGTCAAGTT GCTGAATCAG 
TCATCCGTCA GCCACAAGGA CATCGCCGCC CGCCAAGCTG GGTCATCCAT ATTCCCATGG 
CAACAGCAGG ATCAGGTCCA CAGCCTACGG GGCCAGGATG CGATGGAAAC GCCACTAAAG 
AGACGCGGTG CTTCTCTGTG GGACACCCAC GAGGAAACGC CGATCAAACC CAACCAGCGG 
ATGAGTTCGT CCAGAACCGT CCAGAGTCCC AGTGGATCGA CCCAGCAGAC GGAGCAGCTG 
AAGAGCCTCA ACCAGGAGCT GCGTGGACGT GTGTCAGAGC TGGAGAGGAA TCTGGCTGCT 
CAGGAAAAGG AAATTCGTAA CCAAACATCG AAGCTGCAAG AACTCCAAAC TCACTTGAAC 
CAAACCCGCA AAGAGCTGGC GGAGCGGGAC AGAGAGCTGG CCAAGGCCGG CCACGAGCTG 
AGCCAGGCTG CAGACCGCCA CCAGCAGCTG GAGGCAAAGT GCTCCTTGGT GGAGCAGAAG 
CTGAAGCAGG TCTCCGAGGA GATGAGCTGC CAGAGGCACA ACGCTGAAAG CTGCAGACGA 
GCTCTGGAGC AGAAACTCAA AGACCAAGAA AGAAACAGTC AGAAGGAGTT GGCTCAGCTT 
CAGAGTTCCC ATCAGGCTTT GGATCAGCAG CTCAACCAGA CCAGAACCAA GCTGACACAG 
GAGATCCAAC AGGCCAAAAA AGACCACAAT GTTCTTCAAG CGGACATGGA GAAGACGCGC 
TTGCAGAAGA GTCAGATGGA GAGAGAAATG GAGGAGCAGA AGCAGAAGCT GCTGCGGTCC 
GAACAAGGCC TGCAGGCGAC TCAGACCAAA GAGCAAGACC TCCGCAAAAA GATGGAGGAG 
CTGCAGAAAG AGAAGAACGG CGTGACCATC CAGTTGGACC GCTGCAGCAG GTCGCTGACT 
CAGCTGGAAG AGGAGAAGAG GATCTCAGAG CAGTCGCTGA AACGCACCCA GGGGCTGCTG 
GAGGACCTGA AAGCGAAATC TGAAGGACAG GCCGAGGAGC TGAAGAGACT CCAGTCAAAA 
TTTGAGCAAC AGACTCAGAC GGCAGCAAGA GAGCTGGAAC ACGTGAAGAA GACGCTTTCT 
GACGCAGAGG CCAGGAATGA CAAATCTCAG AATGAGCTTC AAAAGCAGAA GCAGGAGAGT 
GAGAAGCTGA GCAACAAGCT GACGGTCATA GAGCAGGAGA GCGAAGAGCT GAAATCAAAC 
CTCCGTCAGA GTCGGGACGA GTGCCAGGCT GTGAAACAGG AGCACCAGGC TCTGCTGGAG 
TGGAAGAAAA ACAAGGAGAA CTTGATAAAC CAAACCGAGG CCGCGCAGCA GGAGCTCACT 
GACAAGATTA TCACTTTGGA GGGCAAAGCG AGCCGGATGC ATGAGGCCAA CAATGAACTC 
CAGGACAAGA TCTCATCCAC GGAGGCAGAC AAAGCCAGTC TGTCCGCCCA CATCGACGCC 
CTGAAGGGAG AACTCCTCAT GAGGAGCACG GAGCTGGAGG AGAAGGAGCA TCAGTACCAG 
CAGCTTCAGC TCCACGTCTC TGAAGCGGCA CAGAAACACG GCAAAGACCT GGAGAACGCC 
GGGAAGCAGC TGGCGCAGCT CCAAGGACAG GTGAAAGAGC TGGAGTCCCG GCTGCAGAGG 
GAGGCGTCTC GAGCAGAGCT GGCCGAAAAG ACGAACTGCG AGCTGCAGGA GGAGCACCAG 
GCCGCGTGCG ACCTGCTGCG CTCCAAAGAC CAGCTGCTGG AGCTGGGCCG GGCCGAGGTC 
AGCCAGCTGA AGGACAGCCT CGCTCAGTCC GCCGCACAGC AGGAGGCGCA GAGCGACAGC 
TTTGTGAAGC AGAAGGCCGT CCTGCTGAAG CGGTGTGAGG AGAGCATTTC GGCGCAGGCT 
GAGGAGACGG AGCGCGTCAA GCTGCAGGCA GAGGAAGTCC AGCAGGAGCT GCTGCTCTCC 
AGACACAAAA TCACCTCGAT GGAGCAGATC CTGAAGGTCC AGGAACAGCT GGGAGCCGAG 
CTGCAGAAAC AAATTAAGAC GACGTCCGAT AAGGAGGAGG AGCTCACGAA GACGTGCGAG 
GAGAAATCAG AGGAGCTGAA ACGCTTGGAG GAGGAGCTGA AGGACCAGCG GAGCCTCACC 
GAGGAGACGG AGCAGCAGAT CAGAGCGGCT GAAGCTCGGA TCCACTCCGT GGAAAATCAA 
AAGGCTGAGC TGGAAAATCG ACTTCAAGAG ATGAAGACAG AAGCAGAGAT CCTGCAGTTC 
AGTCACACTG AAAGGATGAA CGCTCTCCAG GAACAGATCG TATGTCTGGA AAAGCAGGTT 
GCTGCAAACC AGGAAGAAGC AGAGGAGGTT CCTGTCCTGA AAAACAAACT GGACGTGGTC 
AACCAGTCTC TTGCTCACCT TAACAAATCT CTTGAAGCTT CAGAAAAGAG CCTCAGCTGT 
GCAAATGAAA TAAAAGCTGA TCTGGAGGTC ACCCTGTCTG AGAAAGTGAA ACTTATCACT 
GCTTTGGAGG ATCAGATCAA TAATCTCACT GAGAAGCTGA GGAAAGAGTC TGAGAGCCAC 
AGCTCAGAGG TTGAGGACTT CATCAATAAG GAGAGGAGCC TCAAAGAGCA GCTGGAAGCC 
ACTAGGAAAT CAGTAGCTGC AGCCAAAGAA GAGTCCAGCT CTAGACGGGA GAATATCCGA 
GAAATGAAGA TGACTCTGTC TGCAGCGTCC CGCGGCCTGG AAGAAAGAGA CAACACGATA 
AAGAGTCTGA AAGAGAAGCT GAACAAAGCA GAGGCAGAGC AGGCCAAAGC CTCTGAGCTC 
CTGAAGGAGA AGACGGTGGC CATGAGCAAA ATCAAGGTGC AGCTGGAGAT GCTGCAGATG 
GACCTGGAGG ACAACGAGTT GGCCATGACC TCTGTGGGCA GTCAGGCGGA GGAGCTGAAG 
GGAACCATAG CGTCCCTGGA GGCCATGCTG GAGGAAAGCA AAGCCCAGGT TTCTGTTTTG 
GAGACCCGTT TGGATGAGGT CCAGTACCAA AACTCTCTGC TGGAGACAAA ATACGTGACG 
GCGAAGGACG AGCTGCTGGA GCGGAGCTGC GAGATAACCC GTCTGGAGGA GGACGCCGTC 
AGACGGCAGC AGCTAGAGGA GAACGTCTCT GCCTTGGAGG CCAAACTGAG TCAGATTACT 
GAGGAAAACT CAAAGGCAGA GACGGAGCTG AGGCAGATCA TTCAGGAGAA AGAGAGCCAT 
TTGGAGCAAG TAAACGAGGA GAGAAACAGC CTCCAGGCTA CCTTAACGCA GTCAGTGGAT 
GAGCAGAAAC GAGCTGAATC TGAAATCACT CAGGTAAAAG CGGTGAAGGC GGAGCTGGAG 
GAGAGAATGA GCAACCTGTC GAAGGAGATT CAGCAACTCC ATGCTGATGT TAGAGAGTTA 
TCTGAACAGA AACAGGAAGC GGAGGGAACG ATTGACCAGA TGGCTGCAGA AACACAACAG 
ACAGAGTCCG CTCTCCGTCG GATCTCAGAG GAGAAAGCCC AGCTGGACGT TGCTCTGAGT 
TCAGTCAGCG AGGAAAAAGT TGAGCTGCAG GAGAGGATTT CTACTTTAAC CTCTGAGAAC 
GGGGAGCTGG TCTGCAAAGT GAAGCAGGTG GAGGAGGAGA AACTCCAGCT GGAGTCTAAG 
TTTAGTCAAA TCTCTGAGGA AAACGTCAAG CAGCAGTCAG ACATGAACCG TCTGACTGCA 
GAGAAGCAGC AGCTGCAGAG CAGTGCAGAG AGGCTGCAGG AGGAGATGGC GTCTCTGCGG 
GAGGAGAGCG AGCGTGTCCA GAGCTCGCTG CTGTCCGCTG AGGAGGAGCG ACAGATGCTG 
CGGGAGCAGC TCAACACGAG GGACGAGACC AACCACACGG AGAGCAGAGC GCGCAACCAG 
CAGTTTGAGG CGGAGCAGAC TGCACTTCAT CAGAAGCTGT CCATCCTCCA GACGGAGCTG 
GCTGTGCTGC AGCAGCAGTA CGATTTGCTT CTGCAGCAAG TGGACCAGCA GCAACGCATC 
ATTCAGCAGC TCACAGAATC ACAGAAATAC CAGAATCCGA CTGGAAACAT CCCGCGCCTC 
GAACCCAAAG ACGCTGAGGA TGGCGCAGAG GCAGCTGAAC AGACCAAACC AAACCCAGAA 
CTTGGTGCGA ACGTCGGTAC CAGTGCAGAG TCAGATCCAC TGAATGAACA GGAAGAGGCG 
TCTGAAAAGA GCCCTGATCA GGCTGCGAGT GAGGCAGAGC TGATCGTTCG GGAGGACGCC 
TCTGATCAGG AGATGATCAA TGAGGAGGAG GCTCATAAAC ATGATGCTCA GGAAATCCAA 
CTTCGCCAGC AGGTCTCTGA GGAGAACTGC AGCAGTAGTG GAGATCTTGA AAATGAGAAG 
TATGATGCAT CTTCCTTCCA ACATGAGATA AGAGGCATGA AATTTTCTGG AGACGAGTCT 
TGGCCGGTGA AAGACGTCGA TACAGAAATG ATCTCAGAGC AGCAGAGTGA ACTGAAGACG 
CTGCGGTCGG AGTTTGACCT GCTGACCTCT GAGCTGCAGC TGAGAGCAGA GCTGACCTCA 
GAGCTGGAAG TCCAAGTTCA GAAGTTGGAG GAGAAAGTTC AGGCTGCAGA GGAGCAGCTG 
AGCACCGCTT TGGAGGAGAA GAAGAGTCTT TCTGATCAGG TGACCCAGCT GCTGGAGGAG 
AGGGAGTCAC TTAGTCTGCA GCTGGAAACG TCTAAATGTC AACTCACTGA CTTCATGGAG 
ATGTTGGAAG GACTTGAGAT GGCCAAAGGC GGATTGGATG AGAAGTTCCT TCAGCAGGAG 
AGTGAGTTGA AGAGGGTCCG CTCTGAAAAA GCTAACCTGG AGCAGCACAT CCTGGGAATG 
GAGTCTGAAC TGGAGAGCAT GCAAGCAGAA ACCTCCAGGC TTACGGAGGA GCTGGAGATC 
CAGAGGAAGA CTTGTTCAGG ACGGGAACAA CAGATAGAAA CTCTCCTTAC GGAGACGACC 
CAGCTGAGAG CTGAACTCGT GTCGTGCTCT GAAGACCGAG ATGAGTTGAG TCAGTCTTTA 
GGTCAGTGGA GAGACAAAGT TCACGGTTTG GAGAAGACAA ACCTGGAAAC CAGAAATTTA 
ATCTCCATCC TGGAGGAAGA CATCCGGGTC GGAAGAAAGG AGTACGAAGG TCTGCAAAGC 
GGCATGGAGA GAGTGAAGGC AGAGAGGGAG CAGTTTTTGC AGCAGGTTGT GGTTCTGGAA 
GAGGCCATTT CTAAACATAA CAGGGAGAAG GAGGGGCTGC TCAACCACCT CCATGAGATG 
GAAGAAGATC ACACGTCTAC CAACCAAAAC ACCGAGTCCA TGGCCGGCAA AATCCAGGCG 
TTGGAGGGAG AAGTGTGTCG CCTCTCCCAG TCTCTGGAGT CGTCGCTGCT GGAGAAAGGA 
GAAATCGCGT CTCGGCTGAA CTCCACTCAG GATGAAGTCC GGCAGATGAG GACGGGCATC 
GAGAAGCTGC AGGTCCGCAT CGAGTCAGAC GAGAGGAAGA AGAAGAAGAT GGGAGAGCTG 
CTCAAAGCTG CTCAGAGGAA GTCTGACTCG CTGCAGGATC GCATCGATGC GCTGGAGCGA 
GAGAAAGACG AATTTGAGCA AAATCTAGAG GAGGCCGTGT TGCAGGCTGA AGTAGCAAAA 
GCTGAGCTGG AAGAGGAGAG GACTAAGGTG GAAGAAGAGA AGAAAGAGCT GAATGAGAAA 
CTGACTGAGC TGTCCGCCGC TCTGGACACT CTGACATCTC AGAAAGCTCA TTTAGAGAGA 
GAGCTGGACA TGAAAAATGT AGAGATAGAG GAGCTGAAGG CAGCTAAGGA CAAACTGGAA 
CAGGGTTTGG AGAAAGCAGA GGTGGACAGA AGAGAAGAAG ACAGACAGAG ACAAAGACTA 
GAGGAGCTGG AGAAATGGAC GAGAGAGGAA GCAGAGAGAC AAAGTGTGCG AGTCGGAGAC 
CTTCAGGGCC AGCTGACGGA GTTGGAAAGG GAAAACAACG ACCTTAGGGC AACGTTTGAG 
TCTTTAGGAG AAGAAATAAA GGATCTTAAA ACACTTGCTC AAGCTAAAGA GGAAGAAATC 
CTCGCTTTGG AGAAGGACAA AGAGAATAAG GCAGAATCCA TCTCATTAAT CGTAGCAGAG 
AAGAAACGAT TAGAGGAAAG AAATGAACAG CTGGAAAAGG AGAGAGACGA CCTTCAGTCT 
GCTCTGGTGT CTGTGAACCA AGAGAAAGCA AAGGCAGATC AGGAGAAAAC AGAGTTGGAT 
GAAGGGAGAG TAAAGCTTCA GTCCAAGATC TCGTTGGTAG AAACGGAGAA ACAGAACCTC 
CAACAAACCG TCTCCTTGTT GGAGCAGGAA AAGCTGAGGT CAGAGGCTGA AACTGAGGAG 
TTGCAGTCTT CACTGTTGAT GATGGAAGAG GAGAAGAACA ACCTGTCTTC AGCTCTTTCT 
CTGCTGGAAG AAGAGAAGCA GCAGGCAACA GAGGAGAAAG AGAGGCTCAC ACAGGAACAT 
GAGGTTCTCC AGAAAACAGT CGCCTCCATG GAAGAAGAAC TGGAGACACA CAAACGATCC 
ATGACCCAGC TCAGTGAGCA GGTTTCTGAA CTAACATCCA GTTTAGCTCG TCTGACAAAA 
GAGAGAGACT CTGCTCTGAG CAAAATGAAC CTTTGGATGA AGACCTGCAA ACAGCTGGAG 
CAGGAGAAGC AGAACATCCT AAACAGCTCA GAAGGAAGAA GCAGTGAGGA GTTTCAGGCT 
GAAGCGGCCC AGCTGAGGGC GCAGGCAGAG GTCAGGAAGA AAGAAGTCCA AGAACTGAAA 
ACCGCCCTGG AGAAAAAAAC GACAGAGGCT GAGGAAAGAC GACAAGAATT GAAGCAAAAG 
GAGGAAGAGG TGAAGGAGCG AGCGGCTGCT CTGGAGGGAG TGAGAGCGGA GAAGGAAAGG 
GAGCTGGAGG AGATCAGGAA GGAACTGGAT GAGGTTAACG CGCTTCTGGA GGAGAAAAGC 
ACCGAGGCCG ATGAGAGCAT AGAGAAATAC TGCAGCCTGC TGGTCAAAGT CCACAAGCTG 
GAGGAGAGCA ATGACGCGCT GAAGACCCGA CTGGAGCATC TCACTGCCAG CCAGCCTGTG 
AATGAAGCCA AGGTCCCTCC TGAAACCCGC CGGCGTTCAG TGAGGAAGTC TTCCTCCAAA 
CACCAGGAAG AAAAAATGAG TGAAAACACA GAGAATCTGG TTCCCACGAC AACACCGAGC 
TCTCCACAGG GAGGGTCTCC GGGGAAACGG GGTCATAAAG AAATCAGTGA CAGAGACAGC 
GCCCAGGAGG CTCTGCACAA CCTGACGAAG AAACTAAAAG CCAACACCGC GACGCCCAGA 
GGAAGAGGAG AACAGGATGA CGAAGAGTTC AGACCAGAGG GCCTGCCTGA CCTGGTGCAG 
AAAGGGTTCG CTGATATCCC ACTGGGGGAG GCCAGTCCGT ACATCATCCG GAGGACCACC 
GGGAGGCGCT GCAGTCCTCG CCTGGCTGCA AGGCAGACTA AGCCTGACAT CAAGGTTTTG 
GGGCCCGTCC ATCTGCAGAG TCCCTCTGGT TCATCATCGG ACGGCTCCGA CAGGACGCGC 
CAGCCTCTGA GTGGCGAACA GGAACCTTCG CTGAATGTCC ATCAGGAAGC AGAGCAGAGA 
GAGAAACAGG TTTTAGTGGA GCAAACAAAA CAAGGAGAAA ACTGTCATGT TCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cenpf ( XM_023332348.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023188116.1 Xiphophorus maculatus centromere protein F (cenpf), transcript variant X2, mRNA.

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CloneID	OXi37126
Clone ID Related Accession (Same CDS sequence)	XM_023332348.1
Accession Version	XM_023332348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	centromere protein F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036446.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG CGGTGGAGGA GTGGAAGGAT GGACTTCCAG GGAAAGCCCT GCAGAAGATC 
CAGGAGATGG AGGTCCAACT GGACAAACTG AAGAAGGAAA GGACACAGAA GCAGTTTCAG 
CTGGACTCTC TGGAAGCTGC CCTGCAGAAA CAGAAGCAGA AGGTGGACAG TGAACGCACC 
GAGATCTCCG CTTTGAAAAG AGAAAACCAG TCGATGATTG AGTCATGTGA CGCTTTGGAG 
AAAACTCGTC AGAAGGTGGT TCATGACCTT GGAGTCAAAG AGCAGCAGGT GAGCTACCTG 
GAGGGTCAAC TTAACTCCTG CAGGAAGACA ATAGATCGTC TGGAGCAAGA ACTCAAGAAG 
TACAAAACTG AGCTCGATCG CTCTCAACCT GCCGGATCTT CCTTGCTGTC CACCTCTTCC 
TCTGACCTGC AGACGTTCAG CACGCCACAG AAGACTTTTG CTACGCCAGC GCCTGTCGCT 
GCACACAGAC AGCCAGACAG CAAACTGGAC GAGCTTCAGG AAAAGTACAA CCATGAAGTG 
GAAGAAAGAA GAAAGCTGGA AAGTGAGCTC AAAATGTTGC AGGTCAAGTT GCTGAATCAG 
TCATCCGTCA GCCACAAGGA CATCGCCGCC CGCCAAGCTG GGTCATCCAT ATTCCCATGG 
CAACAGCAGG ATCAGGTCCA CAGCCTACGG GGCCAGGATG CGATGGAAAC GCCACTAAAG 
AGACGCGGTG CTTCTCTGTG GGACACCCAC GAGGAAACGC CGATCAAACC CAACCAGCGG 
ATGAGTTCGT CCAGAACCGT CCAGAGTCCC AGTGGATCGA CCCAGCAGAC GGAGCAGCTG 
AAGAGCCTCA ACCAGGAGCT GCGTGGACGT GTGTCAGAGC TGGAGAGGAA TCTGGCTGCT 
CAGGAAAAGG AAATTCGTAA CCAAACATCG AAGCTGCAAG AACTCCAAAC TCACTTGAAC 
CAAACCCGCA AAGAGCTGGC GGAGCGGGAC AGAGAGCTGG CCAAGGCCGG CCACGAGCTG 
AGCCAGGCTG CAGACCGCCA CCAGCAGCTG GAGGCAAAGT GCTCCTTGGT GGAGCAGAAG 
CTGAAGCAGG TCTCCGAGGA GATGAGCTGC CAGAGGCACA ACGCTGAAAG CTGCAGACGA 
GCTCTGGAGC AGAAACTCAA AGACCAAGAA AGAAACAGTC AGAAGGAGTT GGCTCAGCTT 
CAGAGTTCCC ATCAGGCTTT GGATCAGCAG CTCAACCAGA CCAGAACCAA GCTGACACAG 
GAGATCCAAC AGGCCAAAAA AGACCACAAT GTTCTTCAAG CGGACATGGA GAAGACGCGC 
TTGCAGAAGA GTCAGATGGA GAGAGAAATG GAGGAGCAGA AGCAGAAGCT GCTGCGGTCC 
GAACAAGGCC TGCAGGCGAC TCAGACCAAA GAGCAAGACC TCCGCAAAAA GATGGAGGAG 
CTGCAGAAAG AGAAGAACGG CGTGACCATC CAGTTGGACC GCTGCAGCAG GTCGCTGACT 
CAGCTGGAAG AGGAGAAGAG GATCTCAGAG CAGTCGCTGA AACGCACCCA GGGGCTGCTG 
GAGGACCTGA AAGCGAAATC TGAAGGACAG GCCGAGGAGC TGAAGAGACT CCAGTCAAAA 
TTTGAGCAAC AGACTCAGAC GGCAGCAAGA GAGCTGGAAC ACGTGAAGAA GACGCTTTCT 
GACGCAGAGG CCAGGAATGA CAAATCTCAG AATGAGCTTC AAAAGCAGAA GCAGGAGAGT 
GAGAAGCTGA GCAACAAGCT GACGGTCATA GAGCAGGAGA GCGAAGAGCT GAAATCAAAC 
CTCCGTCAGA GTCGGGACGA GTGCCAGGCT GTGAAACAGG AGCACCAGGC TCTGCTGGAG 
TGGAAGAAAA ACAAGGAGAA CTTGATAAAC CAAACCGAGG CCGCGCAGCA GGAGCTCACT 
GACAAGATTA TCACTTTGGA GGGCAAAGCG AGCCGGATGC ATGAGGCCAA CAATGAACTC 
CAGGACAAGA TCTCATCCAC GGAGGCAGAC AAAGCCAGTC TGTCCGCCCA CATCGACGCC 
CTGAAGGGAG AACTCCTCAT GAGGAGCACG GAGCTGGAGG AGAAGGAGCA TCAGTACCAG 
CAGCTTCAGC TCCACGTCTC TGAAGCGGCA CAGAAACACG GCAAAGACCT GGAGAACGCC 
GGGAAGCAGC TGGCGCAGCT CCAAGGACAG GTGAAAGAGC TGGAGTCCCG GCTGCAGAGG 
GAGGCGTCTC GAGCAGAGCT GGCCGAAAAG ACGAACTGCG AGCTGCAGGA GGAGCACCAG 
GCCGCGTGCG ACCTGCTGCG CTCCAAAGAC CAGCTGCTGG AGCTGGGCCG GGCCGAGGTC 
AGCCAGCTGA AGGACAGCCT CGCTCAGTCC GCCGCACAGC AGGAGGCGCA GAGCGACAGC 
TTTGTGAAGC AGAAGGCCGT CCTGCTGAAG CGGTGTGAGG AGAGCATTTC GGCGCAGGCT 
GAGGAGACGG AGCGCGTCAA GCTGCAGGCA GAGGAAGTCC AGCAGGAGCT GCTGCTCTCC 
AGACACAAAA TCACCTCGAT GGAGCAGATC CTGAAGGTCC AGGAACAGCT GGGAGCCGAG 
CTGCAGAAAC AAATTAAGAC GACGTCCGAT AAGGAGGAGG AGCTCACGAA GACGTGCGAG 
GAGAAATCAG AGGAGCTGAA ACGCTTGGAG GAGGAGCTGA AGGACCAGCG GAGCCTCACC 
GAGGAGACGG AGCAGCAGAT CAGAGCGGCT GAAGCTCGGA TCCACTCCGT GGAAAATCAA 
AAGGCTGAGC TGGAAAATCG ACTTCAAGAG ATGAAGACAG AAGCAGAGAT CCTGCAGTTC 
AGTCACACTG AAAGGATGAA CGCTCTCCAG GAACAGATCG TATGTCTGGA AAAGCAGGTT 
GCTGCAAACC AGGAAGAAGC AGAGGAGGTT CCTGTCCTGA AAAACAAACT GGACGTGGTC 
AACCAGTCTC TTGCTCACCT TAACAAATCT CTTGAAGCTT CAGAAAAGAG CCTCAGCTGT 
GCAAATGAAA TAAAAGCTGA TCTGGAGGTC ACCCTGTCTG AGAAAGTGAA ACTTATCACT 
GCTTTGGAGG ATCAGATCAA TAATCTCACT GAGAAGCTGA GGAAAGAGTC TGAGAGCCAC 
AGCTCAGAGG TTGAGGACTT CATCAATAAG GAGAGGAGCC TCAAAGAGCA GCTGGAAGCC 
ACTAGGAAAT CAGTAGCTGC AGCCAAAGAA GAGTCCAGCT CTAGACGGGA GAATATCCGA 
GAAATGAAGA TGACTCTGTC TGCAGCGTCC CGCGGCCTGG AAGAAAGAGA CAACACGATA 
AAGAGTCTGA AAGAGAAGCT GAACAAAGCA GAGGCAGAGC AGGCCAAAGC CTCTGAGCTC 
CTGAAGGAGA AGACGGTGGC CATGAGCAAA ATCAAGGTGC AGCTGGAGAT GCTGCAGATG 
GACCTGGAGG ACAACGAGTT GGCCATGACC TCTGTGGGCA GTCAGGCGGA GGAGCTGAAG 
GGAACCATAG CGTCCCTGGA GGCCATGCTG GAGGAAAGCA AAGCCCAGGT TTCTGTTTTG 
GAGACCCGTT TGGATGAGGT CCAGTACCAA AACTCTCTGC TGGAGACAAA ATACGTGACG 
GCGAAGGACG AGCTGCTGGA GCGGAGCTGC GAGATAACCC GTCTGGAGGA GGACGCCGTC 
AGACGGCAGC AGCTAGAGGA GAACGTCTCT GCCTTGGAGG CCAAACTGAG TCAGATTACT 
GAGGAAAACT CAAAGGCAGA GACGGAGCTG AGGCAGATCA TTCAGGAGAA AGAGAGCCAT 
TTGGAGCAAG TAAACGAGGA GAGAAACAGC CTCCAGGCTA CCTTAACGCA GTCAGTGGAT 
GAGCAGAAAC GAGCTGAATC TGAAATCACT CAGGTAAAAG CGGTGAAGGC GGAGCTGGAG 
GAGAGAATGA GCAACCTGTC GAAGGAGATT CAGCAACTCC ATGCTGATGT TAGAGAGTTA 
TCTGAACAGA AACAGGAAGC GGAGGGAACG ATTGACCAGA TGGCTGCAGA AACACAACAG 
ACAGAGTCCG CTCTCCGTCG GATCTCAGAG GAGAAAGCCC AGCTGGACGT TGCTCTGAGT 
TCAGTCAGCG AGGAAAAAGT TGAGCTGCAG GAGAGGATTT CTACTTTAAC CTCTGAGAAC 
GGGGAGCTGG TCTGCAAAGT GAAGCAGGTG GAGGAGGAGA AACTCCAGCT GGAGTCTAAG 
TTTAGTCAAA TCTCTGAGGA AAACGTCAAG CAGCAGTCAG ACATGAACCG TCTGACTGCA 
GAGAAGCAGC AGCTGCAGAG CAGTGCAGAG AGGCTGCAGG AGGAGATGGC GTCTCTGCGG 
GAGGAGAGCG AGCGTGTCCA GAGCTCGCTG CTGTCCGCTG AGGAGGAGCG ACAGATGCTG 
CGGGAGCAGC TCAACACGAG GGACGAGACC AACCACACGG AGAGCAGAGC GCGCAACCAG 
CAGTTTGAGG CGGAGCAGAC TGCACTTCAT CAGAAGCTGT CCATCCTCCA GACGGAGCTG 
GCTGTGCTGC AGCAGCAGTA CGATTTGCTT CTGCAGCAAG TGGACCAGCA GCAACGCATC 
ATTCAGCAGC TCACAGAATC ACAGAAATAC CAGAATCCGA CTGGAAACAT CCCGCGCCTC 
GAACCCAAAG ACGCTGAGGA TGGCGCAGAG GCAGCTGAAC AGACCAAACC AAACCCAGAA 
CTTGGTGCGA ACGTCGGTAC CAGTGCAGAG TCAGATCCAC TGAATGAACA GGAAGAGGCG 
TCTGAAAAGA GCCCTGATCA GGCTGCGAGT GAGGCAGAGC TGATCGTTCG GGAGGACGCC 
TCTGATCAGG AGATGATCAA TGAGGAGGAG GCTCATAAAC ATGATGCTCA GGAAATCCAA 
CTTCGCCAGC AGGTCTCTGA GGAGAACTGC AGCAGTAGTG GAGATCTTGA AAATGAGAAG 
TATGATGCAT CTTCCTTCCA ACATGAGATA AGAGGCATGA AATTTTCTGG AGACGAGTCT 
TGGCCGGTGA AAGACGTCGA TACAGAAATG ATCTCAGAGC AGCAGAGTGA ACTGAAGACG 
CTGCGGTCGG AGTTTGACCT GCTGACCTCT GAGCTGCAGC TGAGAGCAGA GCTGACCTCA 
GAGCTGGAAG TCCAAGTTCA GAAGTTGGAG GAGAAAGTTC AGGCTGCAGA GGAGCAGCTG 
AGCACCGCTT TGGAGGAGAA GAAGAGTCTT TCTGATCAGG TGACCCAGCT GCTGGAGGAG 
AGGGAGTCAC TTAGTCTGCA GCTGGAAACG TCTAAATGTC AACTCACTGA CTTCATGGAG 
ATGTTGGAAG GACTTGAGAT GGCCAAAGGC GGATTGGATG AGAAGTTCCT TCAGCAGGAG 
AGTGAGTTGA AGAGGGTCCG CTCTGAAAAA GCTAACCTGG AGCAGCACAT CCTGGGAATG 
GAGTCTGAAC TGGAGAGCAT GCAAGCAGAA ACCTCCAGGC TTACGGAGGA GCTGGAGATC 
CAGAGGAAGA CTTGTTCAGG ACGGGAACAA CAGATAGAAA CTCTCCTTAC GGAGACGACC 
CAGCTGAGAG CTGAACTCGT GTCGTGCTCT GAAGACCGAG ATGAGTTGAG TCAGTCTTTA 
GGTCAGTGGA GAGACAAAGT TCACGGTTTG GAGAAGACAA ACCTGGAAAC CAGAAATTTA 
ATCTCCATCC TGGAGGAAGA CATCCGGGTC GGAAGAAAGG AGTACGAAGG TCTGCAAAGC 
GGCATGGAGA GAGTGAAGGC AGAGAGGGAG CAGTTTTTGC AGCAGGTTGT GGTTCTGGAA 
GAGGCCATTT CTAAACATAA CAGGGAGAAG GAGGGGCTGC TCAACCACCT CCATGAGATG 
GAAGAAGATC ACACGTCTAC CAACCAAAAC ACCGAGTCCA TGGCCGGCAA AATCCAGGCG 
TTGGAGGGAG AAGTGTGTCG CCTCTCCCAG TCTCTGGAGT CGTCGCTGCT GGAGAAAGGA 
GAAATCGCGT CTCGGCTGAA CTCCACTCAG GATGAAGTCC GGCAGATGAG GACGGGCATC 
GAGAAGCTGC AGGTCCGCAT CGAGTCAGAC GAGAGGAAGA AGAAGAAGAT GGGAGAGCTG 
CTCAAAGCTG CTCAGAGGAA GTCTGACTCG CTGCAGGATC GCATCGATGC GCTGGAGCGA 
GAGAAAGACG AATTTGAGCA AAATCTAGAG GAGGCCGTGT TGCAGGCTGA AGTAGCAAAA 
GCTGAGCTGG AAGAGGAGAG GACTAAGGTG GAAGAAGAGA AGAAAGAGCT GAATGAGAAA 
CTGACTGAGC TGTCCGCCGC TCTGGACACT CTGACATCTC AGAAAGCTCA TTTAGAGAGA 
GAGCTGGACA TGAAAAATGT AGAGATAGAG GAGCTGAAGG CAGCTAAGGA CAAACTGGAA 
CAGGGTTTGG AGAAAGCAGA GGTGGACAGA AGAGAAGAAG ACAGACAGAG ACAAAGACTA 
GAGGAGCTGG AGAAATGGAC GAGAGAGGAA GCAGAGAGAC AAAGTGTGCG AGTCGGAGAC 
CTTCAGGGCC AGCTGACGGA GTTGGAAAGG GAAAACAACG ACCTTAGGGC AACGTTTGAG 
TCTTTAGGAG AAGAAATAAA GGATCTTAAA ACACTTGCTC AAGCTAAAGA GGAAGAAATC 
CTCGCTTTGG AGAAGGACAA AGAGAATAAG GCAGAATCCA TCTCATTAAT CGTAGCAGAG 
AAGAAACGAT TAGAGGAAAG AAATGAACAG CTGGAAAAGG AGAGAGACGA CCTTCAGTCT 
GCTCTGGTGT CTGTGAACCA AGAGAAAGCA AAGGCAGATC AGGAGAAAAC AGAGTTGGAT 
GAAGGGAGAG TAAAGCTTCA GTCCAAGATC TCGTTGGTAG AAACGGAGAA ACAGAACCTC 
CAACAAACCG TCTCCTTGTT GGAGCAGGAA AAGCTGAGGT CAGAGGCTGA AACTGAGGAG 
TTGCAGTCTT CACTGTTGAT GATGGAAGAG GAGAAGAACA ACCTGTCTTC AGCTCTTTCT 
CTGCTGGAAG AAGAGAAGCA GCAGGCAACA GAGGAGAAAG AGAGGCTCAC ACAGGAACAT 
GAGGTTCTCC AGAAAACAGT CGCCTCCATG GAAGAAGAAC TGGAGACACA CAAACGATCC 
ATGACCCAGC TCAGTGAGCA GGTTTCTGAA CTAACATCCA GTTTAGCTCG TCTGACAAAA 
GAGAGAGACT CTGCTCTGAG CAAAATGAAC CTTTGGATGA AGACCTGCAA ACAGCTGGAG 
CAGGAGAAGC AGAACATCCT AAACAGCTCA GGAAGAAGCA GTGAGGAGTT TCAGGCTGAA 
GCGGCCCAGC TGAGGGCGCA GGCAGAGGTC AGGAAGAAAG AAGTCCAAGA ACTGAAAACC 
GCCCTGGAGA AAAAAACGAC AGAGGCTGAG GAAAGACGAC AAGAATTGAA GCAAAAGGAG 
GAAGAGGTGA AGGAGCGAGC GGCTGCTCTG GAGGGAGTGA GAGCGGAGAA GGAAAGGGAG 
CTGGAGGAGA TCAGGAAGGA ACTGGATGAG GTTAACGCGC TTCTGGAGGA GAAAAGCACC 
GAGGCCGATG AGAGCATAGA GAAATACTGC AGCCTGCTGG TCAAAGTCCA CAAGCTGGAG 
GAGAGCAATG ACGCGCTGAA GACCCGACTG GAGCATCTCA CTGCCAGCCA GCCTGTGAAT 
GAAGCCAAGG TCCCTCCTGA AACCCGCCGG CGTTCAGTGA GGAAGTCTTC CTCCAAACAC 
CAGGAAGAAA AAATGAGTGA AAACACAGAG AATCTGGTTC CCACGACAAC ACCGAGCTCT 
CCACAGGGAG GGTCTCCGGG GAAACGGGGT CATAAAGAAA TCAGTGACAG AGACAGCGCC 
CAGGAGGCTC TGCACAACCT GACGAAGAAA CTAAAAGCCA ACACCGCGAC GCCCAGAGGA 
AGAGGAGAAC AGGATGACGA AGAGTTCAGA CCAGAGGGCC TGCCTGACCT GGTGCAGAAA 
GGGTTCGCTG ATATCCCACT GGGGGAGGCC AGTCCGTACA TCATCCGGAG GACCACCGGG 
AGGCGCTGCA GTCCTCGCCT GGCTGCAAGG CAGACTAAGC CTGACATCAA GGTTTTGGGG 
CCCGTCCATC TGCAGAGTCC CTCTGGTTCA TCATCGGACG GCTCCGACAG GACGCGCCAG 
CCTCTGAGTG GCGAACAGGA ACCTTCGCTG AATGTCCATC AGGAAGCAGA GCAGAGAGAG 
AAACAGGTTT TAGTGGAGCA AACAAAACAA GGAGAAAACT GTCATGTTCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023188116.1
CDS	53..8266
Translation	MSWAVEEWKDGLPGKALQKIQEMEVQLDKLKKERTQKQFQLDSLEAALQKQKQKVDSERTEISALKRENQSMIESCDALEKTRQKVVHDLGVKEQQVSYLEGQLNSCRKTIDRLEQELKKYKTELDRSQPAGSSLLSTSSSDLQTFSTPQKTFATPAPVAAHRQPDSKLDELQEKYNHEVEERRKLESELKMLQVKLLNQSSVSHKDIAARQAGSSIFPWQQQDQVHSLRGQDAMETPLKRRGASLWDTHEETPIKPNQRMSSSRTVQSPSGSTQQTEQLKSLNQELRGRVSELERNLAAQEKEIRNQTSKLQELQTHLNQTRKELAERDRELAKAGHELSQAADRHQQLEAKCSLVEQKLKQVSEEMSCQRHNAESCRRALEQKLKDQERNSQKELAQLQSSHQALDQQLNQTRTKLTQEIQQAKKDHNVLQADMEKTRLQKSQMEREMEEQKQKLLRSEQGLQATQTKEQDLRKKMEELQKEKNGVTIQLDRCSRSLTQLEEEKRISEQSLKRTQGLLEDLKAKSEGQAEELKRLQSKFEQQTQTAARELEHVKKTLSDAEARNDKSQNELQKQKQESEKLSNKLTVIEQESEELKSNLRQSRDECQAVKQEHQALLEWKKNKENLINQTEAAQQELTDKIITLEGKASRMHEANNELQDKISSTEADKASLSAHIDALKGELLMRSTELEEKEHQYQQLQLHVSEAAQKHGKDLENAGKQLAQLQGQVKELESRLQREASRAELAEKTNCELQEEHQAACDLLRSKDQLLELGRAEVSQLKDSLAQSAAQQEAQSDSFVKQKAVLLKRCEESISAQAEETERVKLQAEEVQQELLLSRHKITSMEQILKVQEQLGAELQKQIKTTSDKEEELTKTCEEKSEELKRLEEELKDQRSLTEETEQQIRAAEARIHSVENQKAELENRLQEMKTEAEILQFSHTERMNALQEQIVCLEKQVAANQEEAEEVPVLKNKLDVVNQSLAHLNKSLEASEKSLSCANEIKADLEVTLSEKVKLITALEDQINNLTEKLRKESESHSSEVEDFINKERSLKEQLEATRKSVAAAKEESSSRRENIREMKMTLSAASRGLEERDNTIKSLKEKLNKAEAEQAKASELLKEKTVAMSKIKVQLEMLQMDLEDNELAMTSVGSQAEELKGTIASLEAMLEESKAQVSVLETRLDEVQYQNSLLETKYVTAKDELLERSCEITRLEEDAVRRQQLEENVSALEAKLSQITEENSKAETELRQIIQEKESHLEQVNEERNSLQATLTQSVDEQKRAESEITQVKAVKAELEERMSNLSKEIQQLHADVRELSEQKQEAEGTIDQMAAETQQTESALRRISEEKAQLDVALSSVSEEKVELQERISTLTSENGELVCKVKQVEEEKLQLESKFSQISEENVKQQSDMNRLTAEKQQLQSSAERLQEEMASLREESERVQSSLLSAEEERQMLREQLNTRDETNHTESRARNQQFEAEQTALHQKLSILQTELAVLQQQYDLLLQQVDQQQRIIQQLTESQKYQNPTGNIPRLEPKDAEDGAEAAEQTKPNPELGANVGTSAESDPLNEQEEASEKSPDQAASEAELIVREDASDQEMINEEEAHKHDAQEIQLRQQVSEENCSSSGDLENEKYDASSFQHEIRGMKFSGDESWPVKDVDTEMISEQQSELKTLRSEFDLLTSELQLRAELTSELEVQVQKLEEKVQAAEEQLSTALEEKKSLSDQVTQLLEERESLSLQLETSKCQLTDFMEMLEGLEMAKGGLDEKFLQQESELKRVRSEKANLEQHILGMESELESMQAETSRLTEELEIQRKTCSGREQQIETLLTETTQLRAELVSCSEDRDELSQSLGQWRDKVHGLEKTNLETRNLISILEEDIRVGRKEYEGLQSGMERVKAEREQFLQQVVVLEEAISKHNREKEGLLNHLHEMEEDHTSTNQNTESMAGKIQALEGEVCRLSQSLESSLLEKGEIASRLNSTQDEVRQMRTGIEKLQVRIESDERKKKKMGELLKAAQRKSDSLQDRIDALEREKDEFEQNLEEAVLQAEVAKAELEEERTKVEEEKKELNEKLTELSAALDTLTSQKAHLERELDMKNVEIEELKAAKDKLEQGLEKAEVDRREEDRQRQRLEELEKWTREEAERQSVRVGDLQGQLTELERENNDLRATFESLGEEIKDLKTLAQAKEEEILALEKDKENKAESISLIVAEKKRLEERNEQLEKERDDLQSALVSVNQEKAKADQEKTELDEGRVKLQSKISLVETEKQNLQQTVSLLEQEKLRSEAETEELQSSLLMMEEEKNNLSSALSLLEEEKQQATEEKERLTQEHEVLQKTVASMEEELETHKRSMTQLSEQVSELTSSLARLTKERDSALSKMNLWMKTCKQLEQEKQNILNSSGRSSEEFQAEAAQLRAQAEVRKKEVQELKTALEKKTTEAEERRQELKQKEEEVKERAAALEGVRAEKERELEEIRKELDEVNALLEEKSTEADESIEKYCSLLVKVHKLEESNDALKTRLEHLTASQPVNEAKVPPETRRRSVRKSSSKHQEEKMSENTENLVPTTTPSSPQGGSPGKRGHKEISDRDSAQEALHNLTKKLKANTATPRGRGEQDDEEFRPEGLPDLVQKGFADIPLGEASPYIIRRTTGRRCSPRLAARQTKPDIKVLGPVHLQSPSGSSSDGSDRTRQPLSGEQEPSLNVHQEAEQREKQVLVEQTKQGENCHVQ

Target ORF information:

RefSeq Version	XM_023332348.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus centromere protein F (cenpf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023332348.1

ORF Insert Sequence:

ATGAGCTGGG CGGTGGAGGA GTGGAAGGAT GGACTTCCAG GGAAAGCCCT GCAGAAGATC 
CAGGAGATGG AGGTCCAACT GGACAAACTG AAGAAGGAAA GGACACAGAA GCAGTTTCAG 
CTGGACTCTC TGGAAGCTGC CCTGCAGAAA CAGAAGCAGA AGGTGGACAG TGAACGCACC 
GAGATCTCCG CTTTGAAAAG AGAAAACCAG TCGATGATTG AGTCATGTGA CGCTTTGGAG 
AAAACTCGTC AGAAGGTGGT TCATGACCTT GGAGTCAAAG AGCAGCAGGT GAGCTACCTG 
GAGGGTCAAC TTAACTCCTG CAGGAAGACA ATAGATCGTC TGGAGCAAGA ACTCAAGAAG 
TACAAAACTG AGCTCGATCG CTCTCAACCT GCCGGATCTT CCTTGCTGTC CACCTCTTCC 
TCTGACCTGC AGACGTTCAG CACGCCACAG AAGACTTTTG CTACGCCAGC GCCTGTCGCT 
GCACACAGAC AGCCAGACAG CAAACTGGAC GAGCTTCAGG AAAAGTACAA CCATGAAGTG 
GAAGAAAGAA GAAAGCTGGA AAGTGAGCTC AAAATGTTGC AGGTCAAGTT GCTGAATCAG 
TCATCCGTCA GCCACAAGGA CATCGCCGCC CGCCAAGCTG GGTCATCCAT ATTCCCATGG 
CAACAGCAGG ATCAGGTCCA CAGCCTACGG GGCCAGGATG CGATGGAAAC GCCACTAAAG 
AGACGCGGTG CTTCTCTGTG GGACACCCAC GAGGAAACGC CGATCAAACC CAACCAGCGG 
ATGAGTTCGT CCAGAACCGT CCAGAGTCCC AGTGGATCGA CCCAGCAGAC GGAGCAGCTG 
AAGAGCCTCA ACCAGGAGCT GCGTGGACGT GTGTCAGAGC TGGAGAGGAA TCTGGCTGCT 
CAGGAAAAGG AAATTCGTAA CCAAACATCG AAGCTGCAAG AACTCCAAAC TCACTTGAAC 
CAAACCCGCA AAGAGCTGGC GGAGCGGGAC AGAGAGCTGG CCAAGGCCGG CCACGAGCTG 
AGCCAGGCTG CAGACCGCCA CCAGCAGCTG GAGGCAAAGT GCTCCTTGGT GGAGCAGAAG 
CTGAAGCAGG TCTCCGAGGA GATGAGCTGC CAGAGGCACA ACGCTGAAAG CTGCAGACGA 
GCTCTGGAGC AGAAACTCAA AGACCAAGAA AGAAACAGTC AGAAGGAGTT GGCTCAGCTT 
CAGAGTTCCC ATCAGGCTTT GGATCAGCAG CTCAACCAGA CCAGAACCAA GCTGACACAG 
GAGATCCAAC AGGCCAAAAA AGACCACAAT GTTCTTCAAG CGGACATGGA GAAGACGCGC 
TTGCAGAAGA GTCAGATGGA GAGAGAAATG GAGGAGCAGA AGCAGAAGCT GCTGCGGTCC 
GAACAAGGCC TGCAGGCGAC TCAGACCAAA GAGCAAGACC TCCGCAAAAA GATGGAGGAG 
CTGCAGAAAG AGAAGAACGG CGTGACCATC CAGTTGGACC GCTGCAGCAG GTCGCTGACT 
CAGCTGGAAG AGGAGAAGAG GATCTCAGAG CAGTCGCTGA AACGCACCCA GGGGCTGCTG 
GAGGACCTGA AAGCGAAATC TGAAGGACAG GCCGAGGAGC TGAAGAGACT CCAGTCAAAA 
TTTGAGCAAC AGACTCAGAC GGCAGCAAGA GAGCTGGAAC ACGTGAAGAA GACGCTTTCT 
GACGCAGAGG CCAGGAATGA CAAATCTCAG AATGAGCTTC AAAAGCAGAA GCAGGAGAGT 
GAGAAGCTGA GCAACAAGCT GACGGTCATA GAGCAGGAGA GCGAAGAGCT GAAATCAAAC 
CTCCGTCAGA GTCGGGACGA GTGCCAGGCT GTGAAACAGG AGCACCAGGC TCTGCTGGAG 
TGGAAGAAAA ACAAGGAGAA CTTGATAAAC CAAACCGAGG CCGCGCAGCA GGAGCTCACT 
GACAAGATTA TCACTTTGGA GGGCAAAGCG AGCCGGATGC ATGAGGCCAA CAATGAACTC 
CAGGACAAGA TCTCATCCAC GGAGGCAGAC AAAGCCAGTC TGTCCGCCCA CATCGACGCC 
CTGAAGGGAG AACTCCTCAT GAGGAGCACG GAGCTGGAGG AGAAGGAGCA TCAGTACCAG 
CAGCTTCAGC TCCACGTCTC TGAAGCGGCA CAGAAACACG GCAAAGACCT GGAGAACGCC 
GGGAAGCAGC TGGCGCAGCT CCAAGGACAG GTGAAAGAGC TGGAGTCCCG GCTGCAGAGG 
GAGGCGTCTC GAGCAGAGCT GGCCGAAAAG ACGAACTGCG AGCTGCAGGA GGAGCACCAG 
GCCGCGTGCG ACCTGCTGCG CTCCAAAGAC CAGCTGCTGG AGCTGGGCCG GGCCGAGGTC 
AGCCAGCTGA AGGACAGCCT CGCTCAGTCC GCCGCACAGC AGGAGGCGCA GAGCGACAGC 
TTTGTGAAGC AGAAGGCCGT CCTGCTGAAG CGGTGTGAGG AGAGCATTTC GGCGCAGGCT 
GAGGAGACGG AGCGCGTCAA GCTGCAGGCA GAGGAAGTCC AGCAGGAGCT GCTGCTCTCC 
AGACACAAAA TCACCTCGAT GGAGCAGATC CTGAAGGTCC AGGAACAGCT GGGAGCCGAG 
CTGCAGAAAC AAATTAAGAC GACGTCCGAT AAGGAGGAGG AGCTCACGAA GACGTGCGAG 
GAGAAATCAG AGGAGCTGAA ACGCTTGGAG GAGGAGCTGA AGGACCAGCG GAGCCTCACC 
GAGGAGACGG AGCAGCAGAT CAGAGCGGCT GAAGCTCGGA TCCACTCCGT GGAAAATCAA 
AAGGCTGAGC TGGAAAATCG ACTTCAAGAG ATGAAGACAG AAGCAGAGAT CCTGCAGTTC 
AGTCACACTG AAAGGATGAA CGCTCTCCAG GAACAGATCG TATGTCTGGA AAAGCAGGTT 
GCTGCAAACC AGGAAGAAGC AGAGGAGGTT CCTGTCCTGA AAAACAAACT GGACGTGGTC 
AACCAGTCTC TTGCTCACCT TAACAAATCT CTTGAAGCTT CAGAAAAGAG CCTCAGCTGT 
GCAAATGAAA TAAAAGCTGA TCTGGAGGTC ACCCTGTCTG AGAAAGTGAA ACTTATCACT 
GCTTTGGAGG ATCAGATCAA TAATCTCACT GAGAAGCTGA GGAAAGAGTC TGAGAGCCAC 
AGCTCAGAGG TTGAGGACTT CATCAATAAG GAGAGGAGCC TCAAAGAGCA GCTGGAAGCC 
ACTAGGAAAT CAGTAGCTGC AGCCAAAGAA GAGTCCAGCT CTAGACGGGA GAATATCCGA 
GAAATGAAGA TGACTCTGTC TGCAGCGTCC CGCGGCCTGG AAGAAAGAGA CAACACGATA 
AAGAGTCTGA AAGAGAAGCT GAACAAAGCA GAGGCAGAGC AGGCCAAAGC CTCTGAGCTC 
CTGAAGGAGA AGACGGTGGC CATGAGCAAA ATCAAGGTGC AGCTGGAGAT GCTGCAGATG 
GACCTGGAGG ACAACGAGTT GGCCATGACC TCTGTGGGCA GTCAGGCGGA GGAGCTGAAG 
GGAACCATAG CGTCCCTGGA GGCCATGCTG GAGGAAAGCA AAGCCCAGGT TTCTGTTTTG 
GAGACCCGTT TGGATGAGGT CCAGTACCAA AACTCTCTGC TGGAGACAAA ATACGTGACG 
GCGAAGGACG AGCTGCTGGA GCGGAGCTGC GAGATAACCC GTCTGGAGGA GGACGCCGTC 
AGACGGCAGC AGCTAGAGGA GAACGTCTCT GCCTTGGAGG CCAAACTGAG TCAGATTACT 
GAGGAAAACT CAAAGGCAGA GACGGAGCTG AGGCAGATCA TTCAGGAGAA AGAGAGCCAT 
TTGGAGCAAG TAAACGAGGA GAGAAACAGC CTCCAGGCTA CCTTAACGCA GTCAGTGGAT 
GAGCAGAAAC GAGCTGAATC TGAAATCACT CAGGTAAAAG CGGTGAAGGC GGAGCTGGAG 
GAGAGAATGA GCAACCTGTC GAAGGAGATT CAGCAACTCC ATGCTGATGT TAGAGAGTTA 
TCTGAACAGA AACAGGAAGC GGAGGGAACG ATTGACCAGA TGGCTGCAGA AACACAACAG 
ACAGAGTCCG CTCTCCGTCG GATCTCAGAG GAGAAAGCCC AGCTGGACGT TGCTCTGAGT 
TCAGTCAGCG AGGAAAAAGT TGAGCTGCAG GAGAGGATTT CTACTTTAAC CTCTGAGAAC 
GGGGAGCTGG TCTGCAAAGT GAAGCAGGTG GAGGAGGAGA AACTCCAGCT GGAGTCTAAG 
TTTAGTCAAA TCTCTGAGGA AAACGTCAAG CAGCAGTCAG ACATGAACCG TCTGACTGCA 
GAGAAGCAGC AGCTGCAGAG CAGTGCAGAG AGGCTGCAGG AGGAGATGGC GTCTCTGCGG 
GAGGAGAGCG AGCGTGTCCA GAGCTCGCTG CTGTCCGCTG AGGAGGAGCG ACAGATGCTG 
CGGGAGCAGC TCAACACGAG GGACGAGACC AACCACACGG AGAGCAGAGC GCGCAACCAG 
CAGTTTGAGG CGGAGCAGAC TGCACTTCAT CAGAAGCTGT CCATCCTCCA GACGGAGCTG 
GCTGTGCTGC AGCAGCAGTA CGATTTGCTT CTGCAGCAAG TGGACCAGCA GCAACGCATC 
ATTCAGCAGC TCACAGAATC ACAGAAATAC CAGAATCCGA CTGGAAACAT CCCGCGCCTC 
GAACCCAAAG ACGCTGAGGA TGGCGCAGAG GCAGCTGAAC AGACCAAACC AAACCCAGAA 
CTTGGTGCGA ACGTCGGTAC CAGTGCAGAG TCAGATCCAC TGAATGAACA GGAAGAGGCG 
TCTGAAAAGA GCCCTGATCA GGCTGCGAGT GAGGCAGAGC TGATCGTTCG GGAGGACGCC 
TCTGATCAGG AGATGATCAA TGAGGAGGAG GCTCATAAAC ATGATGCTCA GGAAATCCAA 
CTTCGCCAGC AGGTCTCTGA GGAGAACTGC AGCAGTAGTG GAGATCTTGA AAATGAGAAG 
TATGATGCAT CTTCCTTCCA ACATGAGATA AGAGGCATGA AATTTTCTGG AGACGAGTCT 
TGGCCGGTGA AAGACGTCGA TACAGAAATG ATCTCAGAGC AGCAGAGTGA ACTGAAGACG 
CTGCGGTCGG AGTTTGACCT GCTGACCTCT GAGCTGCAGC TGAGAGCAGA GCTGACCTCA 
GAGCTGGAAG TCCAAGTTCA GAAGTTGGAG GAGAAAGTTC AGGCTGCAGA GGAGCAGCTG 
AGCACCGCTT TGGAGGAGAA GAAGAGTCTT TCTGATCAGG TGACCCAGCT GCTGGAGGAG 
AGGGAGTCAC TTAGTCTGCA GCTGGAAACG TCTAAATGTC AACTCACTGA CTTCATGGAG 
ATGTTGGAAG GACTTGAGAT GGCCAAAGGC GGATTGGATG AGAAGTTCCT TCAGCAGGAG 
AGTGAGTTGA AGAGGGTCCG CTCTGAAAAA GCTAACCTGG AGCAGCACAT CCTGGGAATG 
GAGTCTGAAC TGGAGAGCAT GCAAGCAGAA ACCTCCAGGC TTACGGAGGA GCTGGAGATC 
CAGAGGAAGA CTTGTTCAGG ACGGGAACAA CAGATAGAAA CTCTCCTTAC GGAGACGACC 
CAGCTGAGAG CTGAACTCGT GTCGTGCTCT GAAGACCGAG ATGAGTTGAG TCAGTCTTTA 
GGTCAGTGGA GAGACAAAGT TCACGGTTTG GAGAAGACAA ACCTGGAAAC CAGAAATTTA 
ATCTCCATCC TGGAGGAAGA CATCCGGGTC GGAAGAAAGG AGTACGAAGG TCTGCAAAGC 
GGCATGGAGA GAGTGAAGGC AGAGAGGGAG CAGTTTTTGC AGCAGGTTGT GGTTCTGGAA 
GAGGCCATTT CTAAACATAA CAGGGAGAAG GAGGGGCTGC TCAACCACCT CCATGAGATG 
GAAGAAGATC ACACGTCTAC CAACCAAAAC ACCGAGTCCA TGGCCGGCAA AATCCAGGCG 
TTGGAGGGAG AAGTGTGTCG CCTCTCCCAG TCTCTGGAGT CGTCGCTGCT GGAGAAAGGA 
GAAATCGCGT CTCGGCTGAA CTCCACTCAG GATGAAGTCC GGCAGATGAG GACGGGCATC 
GAGAAGCTGC AGGTCCGCAT CGAGTCAGAC GAGAGGAAGA AGAAGAAGAT GGGAGAGCTG 
CTCAAAGCTG CTCAGAGGAA GTCTGACTCG CTGCAGGATC GCATCGATGC GCTGGAGCGA 
GAGAAAGACG AATTTGAGCA AAATCTAGAG GAGGCCGTGT TGCAGGCTGA AGTAGCAAAA 
GCTGAGCTGG AAGAGGAGAG GACTAAGGTG GAAGAAGAGA AGAAAGAGCT GAATGAGAAA 
CTGACTGAGC TGTCCGCCGC TCTGGACACT CTGACATCTC AGAAAGCTCA TTTAGAGAGA 
GAGCTGGACA TGAAAAATGT AGAGATAGAG GAGCTGAAGG CAGCTAAGGA CAAACTGGAA 
CAGGGTTTGG AGAAAGCAGA GGTGGACAGA AGAGAAGAAG ACAGACAGAG ACAAAGACTA 
GAGGAGCTGG AGAAATGGAC GAGAGAGGAA GCAGAGAGAC AAAGTGTGCG AGTCGGAGAC 
CTTCAGGGCC AGCTGACGGA GTTGGAAAGG GAAAACAACG ACCTTAGGGC AACGTTTGAG 
TCTTTAGGAG AAGAAATAAA GGATCTTAAA ACACTTGCTC AAGCTAAAGA GGAAGAAATC 
CTCGCTTTGG AGAAGGACAA AGAGAATAAG GCAGAATCCA TCTCATTAAT CGTAGCAGAG 
AAGAAACGAT TAGAGGAAAG AAATGAACAG CTGGAAAAGG AGAGAGACGA CCTTCAGTCT 
GCTCTGGTGT CTGTGAACCA AGAGAAAGCA AAGGCAGATC AGGAGAAAAC AGAGTTGGAT 
GAAGGGAGAG TAAAGCTTCA GTCCAAGATC TCGTTGGTAG AAACGGAGAA ACAGAACCTC 
CAACAAACCG TCTCCTTGTT GGAGCAGGAA AAGCTGAGGT CAGAGGCTGA AACTGAGGAG 
TTGCAGTCTT CACTGTTGAT GATGGAAGAG GAGAAGAACA ACCTGTCTTC AGCTCTTTCT 
CTGCTGGAAG AAGAGAAGCA GCAGGCAACA GAGGAGAAAG AGAGGCTCAC ACAGGAACAT 
GAGGTTCTCC AGAAAACAGT CGCCTCCATG GAAGAAGAAC TGGAGACACA CAAACGATCC 
ATGACCCAGC TCAGTGAGCA GGTTTCTGAA CTAACATCCA GTTTAGCTCG TCTGACAAAA 
GAGAGAGACT CTGCTCTGAG CAAAATGAAC CTTTGGATGA AGACCTGCAA ACAGCTGGAG 
CAGGAGAAGC AGAACATCCT AAACAGCTCA GGAAGAAGCA GTGAGGAGTT TCAGGCTGAA 
GCGGCCCAGC TGAGGGCGCA GGCAGAGGTC AGGAAGAAAG AAGTCCAAGA ACTGAAAACC 
GCCCTGGAGA AAAAAACGAC AGAGGCTGAG GAAAGACGAC AAGAATTGAA GCAAAAGGAG 
GAAGAGGTGA AGGAGCGAGC GGCTGCTCTG GAGGGAGTGA GAGCGGAGAA GGAAAGGGAG 
CTGGAGGAGA TCAGGAAGGA ACTGGATGAG GTTAACGCGC TTCTGGAGGA GAAAAGCACC 
GAGGCCGATG AGAGCATAGA GAAATACTGC AGCCTGCTGG TCAAAGTCCA CAAGCTGGAG 
GAGAGCAATG ACGCGCTGAA GACCCGACTG GAGCATCTCA CTGCCAGCCA GCCTGTGAAT 
GAAGCCAAGG TCCCTCCTGA AACCCGCCGG CGTTCAGTGA GGAAGTCTTC CTCCAAACAC 
CAGGAAGAAA AAATGAGTGA AAACACAGAG AATCTGGTTC CCACGACAAC ACCGAGCTCT 
CCACAGGGAG GGTCTCCGGG GAAACGGGGT CATAAAGAAA TCAGTGACAG AGACAGCGCC 
CAGGAGGCTC TGCACAACCT GACGAAGAAA CTAAAAGCCA ACACCGCGAC GCCCAGAGGA 
AGAGGAGAAC AGGATGACGA AGAGTTCAGA CCAGAGGGCC TGCCTGACCT GGTGCAGAAA 
GGGTTCGCTG ATATCCCACT GGGGGAGGCC AGTCCGTACA TCATCCGGAG GACCACCGGG 
AGGCGCTGCA GTCCTCGCCT GGCTGCAAGG CAGACTAAGC CTGACATCAA GGTTTTGGGG 
CCCGTCCATC TGCAGAGTCC CTCTGGTTCA TCATCGGACG GCTCCGACAG GACGCGCCAG 
CCTCTGAGTG GCGAACAGGA ACCTTCGCTG AATGTCCATC AGGAAGCAGA GCAGAGAGAG 
AAACAGGTTT TAGTGGAGCA AACAAAACAA GGAGAAAACT GTCATGTTCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cenpf ( XM_023332349.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023188117.1 Xiphophorus maculatus centromere protein F (cenpf), transcript variant X3, mRNA.

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CloneID	OXi37127
Clone ID Related Accession (Same CDS sequence)	XM_023332349.1
Accession Version	XM_023332349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	centromere protein F isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036446.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG CGGTGGAGGA GTGGAAGGAT GGACTTCCAG GGAAAGCCCT GCAGAAGATC 
CAGGAGATGG AGGTCCAACT GGACAAACTG AAGAAGGAAA GGACACAGAA GCAGTTTCAG 
CTGGACTCTC TGGAAGCTGC CCTGCAGAAA CAGAAGCAGA AGGTGGACAG TGAACGCACC 
GAGATCTCCG CTTTGAAAAG AGAAAACCAG TCGATGATTG AGTCATGTGA CGCTTTGGAG 
AAAACTCGTC AGAAGGTGGT TCATGACCTT GGAGTCAAAG AGCAGCAGGT GAGCTACCTG 
GAGGGTCAAC TTAACTCCTG CAGGAAGACA ATAGATCGTC TGGAGCAAGA ACTCAAGAAG 
TACAAAACTG AGCTCGATCG CTCTCAACCT GCCGGATCTT CCTTGCTGTC CACCTCTTCC 
TCTGACCTGC AGACGTTCAG CACGCCACAG AAGACTTTTG CTACGCCAGC GCCTGTCGCT 
GCACACAGAC AGCCAGACAG CAAACTGGAC GAGCTTCAGG AAAAGTACAA CCATGAAGTG 
GAAGAAAGAA GAAAGCTGGA AAGTGAGCTC AAAATGTTGC AGGTCAAGTT GCTGAATCAG 
TCATCCGTCA GCCACAAGGA CATCGCCGCC CGCCAAGCTG GGTCATCCAT ATTCCCATGG 
CAACAGCAGG ATCAGGTCCA CAGCCTACGG GGCCAGGATG CGATGGAAAC GCCACTAAAG 
AGACGCGGTG CTTCTCTGTG GGACACCCAC GAGGAAACGC CGATCAAACC CAACCAGCGG 
ATGAGTTCGT CCAGAACCGT CCAGAGTCCC AGTGGATCGA CCCAGCAGAC GGAGCAGCTG 
AAGAGCCTCA ACCAGGAGCT GCGTGGACGT GTGTCAGAGC TGGAGAGGAA TCTGGCTGCT 
CAGGAAAAGG AAATTCGTAA CCAAACATCG AAGCTGCAAG AACTCCAAAC TCACTTGAAC 
CAAACCCGCA AAGAGCTGGC GGAGCGGGAC AGAGAGCTGG CCAAGGCCGG CCACGAGCTG 
AGCCAGGCTG CAGACCGCCA CCAGCAGCTG GAGGCAAAGT GCTCCTTGGT GGAGCAGAAG 
CTGAAGCAGG TCTCCGAGGA GATGAGCTGC CAGAGGCACA ACGCTGAAAG CTGCAGACGA 
GCTCTGGAGC AGAAACTCAA AGACCAAGAA AGAAACAGTC AGAAGGAGTT GGCTCAGCTT 
CAGAGTTCCC ATCAGGCTTT GGATCAGCAG CTCAACCAGA CCAGAACCAA GCTGACACAG 
GAGATCCAAC AGGCCAAAAA AGACCACAAT GTTCTTCAAG CGGACATGGA GAAGACGCGC 
TTGCAGAAGA GTCAGATGGA GAGAGAAATG GAGGAGCAGA AGCAGAAGCT GCTGCGGTCC 
GAACAAGGCC TGCAGGCGAC TCAGACCAAA GAGCAAGACC TCCGCAAAAA GATGGAGGAG 
CTGCAGAAAG AGAAGAACGG CGTGACCATC CAGTTGGACC GCTGCAGCAG GTCGCTGACT 
CAGCTGGAAG AGGAGAAGAG GATCTCAGAG CAGTCGCTGA AACGCACCCA GGGGCTGCTG 
GAGGACCTGA AAGCGAAATC TGAAGGACAG GCCGAGGAGC TGAAGAGACT CCAGTCAAAA 
TTTGAGCAAC AGACTCAGAC GGCAGCAAGA GAGCTGGAAC ACGTGAAGAA GACGCTTTCT 
GACGCAGAGG CCAGGAATGA CAAATCTCAG AATGAGCTTC AAAAGCAGAA GCAGGAGAGT 
GAGAAGCTGA GCAACAAGCT GACGGTCATA GAGCAGGAGA GCGAAGAGCT GAAATCAAAC 
CTCCGTCAGA GTCGGGACGA GTGCCAGGCT GTGAAACAGG AGCACCAGGC TCTGCTGGAG 
TGGAAGAAAA ACAAGGAGAA CTTGATAAAC CAAACCGAGG CCGCGCAGCA GGAGCTCACT 
GACAAGATTA TCACTTTGGA GGGCAAAGCG AGCCGGATGC ATGAGGCCAA CAATGAACTC 
CAGGACAAGA TCTCATCCAC GGAGGCAGAC AAAGCCAGTC TGTCCGCCCA CATCGACGCC 
CTGAAGGGAG AACTCCTCAT GAGGAGCACG GAGCTGGAGG AGAAGGAGCA TCAGTACCAG 
CAGCTTCAGC TCCACGTCTC TGAAGCGGCA CAGAAACACG GCAAAGACCT GGAGAACGCC 
GGGAAGCAGC TGGCGCAGCT CCAAGGACAG GTGAAAGAGC TGGAGTCCCG GCTGCAGAGG 
GAGGCGTCTC GAGCAGAGCT GGCCGAAAAG ACGAACTGCG AGCTGCAGGA GGAGCACCAG 
GCCGCGTGCG ACCTGCTGCG CTCCAAAGAC CAGCTGCTGG AGCTGGGCCG GGCCGAGGTC 
AGCCAGCTGA AGGACAGCCT CGCTCAGTCC GCCGCACAGC AGGAGGCGCA GAGCGACAGC 
TTTGTGAAGC AGAAGGCCGT CCTGCTGAAG CGGTGTGAGG AGAGCATTTC GGCGCAGGCT 
GAGGAGACGG AGCGCGTCAA GCTGCAGGCA GAGGAAGTCC AGCAGGAGCT GCTGCTCTCC 
AGACACAAAA TCACCTCGAT GGAGCAGATC CTGAAGGTCC AGGAACAGCT GGGAGCCGAG 
CTGCAGAAAC AAATTAAGAC GACGTCCGAT AAGGAGGAGG AGCTCACGAA GACGTGCGAG 
GAGAAATCAG AGGAGCTGAA ACGCTTGGAG GAGGAGCTGA AGGACCAGCG GAGCCTCACC 
GAGGAGACGG AGCAGCAGAT CAGAGCGGCT GAAGCTCGGA TCCACTCCGT GGAAAATCAA 
AAGGCTGAGC TGGAAAATCG ACTTCAAGAG ATGAAGACAG AAGCAGAGAT CCTGCAGTTC 
AGTCACACTG AAAGGATGAA CGCTCTCCAG GAACAGATCG TATGTCTGGA AAAGCAGGTT 
GCTGCAAACC AGGAAGAAGC AGAGGAGGTT CCTGTCCTGA AAAACAAACT GGACGTGGTC 
AACCAGTCTC TTGCTCACCT TAACAAATCT CTTGAAGCTT CAGAAAAGAG CCTCAGCTGT 
GCAAATGAAA TAAAAGCTGA TCTGGAGGTC ACCCTGTCTG AGAAAGTGAA ACTTATCACT 
GCTTTGGAGG ATCAGATCAA TAATCTCACT GAGAAGCTGA GGAAAGAGTC TGAGAGCCAC 
AGCTCAGAGG TTGAGGACTT CATCAATAAG GAGAGGAGCC TCAAAGAGCA GCTGGAAGCC 
ACTAGGAAAT CAGTAGCTGC AGCCAAAGAA GAGTCCAGCT CTAGACGGGA GAATATCCGA 
GAAATGAAGA TGACTCTGTC TGCAGCGTCC CGCGGCCTGG AAGAAAGAGA CAACACGATA 
AAGAGTCTGA AAGAGAAGCT GAACAAAGCA GAGGCAGAGC AGGCCAAAGC CTCTGAGCTC 
CTGAAGGAGA AGACGGTGGC CATGAGCAAA ATCAAGGTGC AGCTGGAGAT GCTGCAGATG 
GACCTGGAGG ACAACGAGTT GGCCATGACC TCTGTGGGCA GTCAGGCGGA GGAGCTGAAG 
GGAACCATAG CGTCCCTGGA GGCCATGCTG GAGGAAAGCA AAGCCCAGGT TTCTGTTTTG 
GAGACCCGTT TGGATGAGGT CCAGTACCAA AACTCTCTGC TGGAGACAAA ATACGTGACG 
GCGAAGGACG AGCTGCTGGA GCGGAGCTGC GAGATAACCC GTCTGGAGGA GGACGCCGTC 
AGACGGCAGC AGCTAGAGGA GAACGTCTCT GCCTTGGAGG CCAAACTGAG TCAGATTACT 
GAGGAAAACT CAAAGGCAGA GACGGAGCTG AGGCAGATCA TTCAGGAGAA AGAGAGCCAT 
TTGGAGCAAG TAAACGAGGA GAGAAACAGC CTCCAGGCTA CCTTAACGCA GTCAGTGGAT 
GAGCAGAAAC GAGCTGAATC TGAAATCACT CAGGTAAAAG CGGTGAAGGC GGAGCTGGAG 
GAGAGAATGA GCAACCTGTC GAAGGAGATT CAGCAACTCC ATGCTGATGT TAGAGAGTTA 
TCTGAACAGA AACAGGAAGC GGAGGGAACG ATTGACCAGA TGGCTGCAGA AACACAACAG 
ACAGAGTCCG CTCTCCGTCG GATCTCAGAG GAGAAAGCCC AGCTGGACGT TGCTCTGAGT 
TCAGTCAGCG AGGAAAAAGT TGAGCTGCAG GAGAGGATTT CTACTTTAAC CTCTGAGAAC 
GGGGAGCTGG TCTGCAAAGT GAAGCAGGTG GAGGAGGAGA AACTCCAGCT GGAGTCTAAG 
TTTAGTCAAA TCTCTGAGGA AAACGTCAAG CAGCAGTCAG ACATGAACCG TCTGACTGCA 
GAGAAGCAGC AGCTGCAGAG CAGTGCAGAG AGGCTGCAGG AGGAGATGGC GTCTCTGCGG 
GAGGAGAGCG AGCGTGTCCA GAGCTCGCTG CTGTCCGCTG AGGAGGAGCG ACAGATGCTG 
CGGGAGCAGC TCAACACGAG GGACGAGACC AACCACACGG AGAGCAGAGC GCGCAACCAG 
CAGTTTGAGG CGGAGCAGAC TGCACTTCAT CAGAAGCTGT CCATCCTCCA GACGGAGCTG 
GCTGTGCTGC AGCAGCAGTA CGATTTGCTT CTGCAGCAAG TGGACCAGCA GCAACGCATC 
ATTCAGCAGC TCACAGAATC ACAGAAATAC CAGAATCCGA CTGGAAACAT CCCGCGCCTC 
GAACCCAAAG ACGCTGAGGA TGGCGAGGCA GCTGAACAGA CCAAACCAAA CCCAGAACTT 
GGTGCGAACG TCGGTACCAG TGCAGAGTCA GATCCACTGA ATGAACAGGA AGAGGCGTCT 
GAAAAGAGCC CTGATCAGGC TGCGAGTGAG GCAGAGCTGA TCGTTCGGGA GGACGCCTCT 
GATCAGGAGA TGATCAATGA GGAGGAGGCT CATAAACATG ATGCTCAGGA AATCCAACTT 
CGCCAGCAGG TCTCTGAGGA GAACTGCAGC AGTAGTGGAG ATCTTGAAAA TGAGAAGTAT 
GATGCATCTT CCTTCCAACA TGAGATAAGA GGCATGAAAT TTTCTGGAGA CGAGTCTTGG 
CCGGTGAAAG ACGTCGATAC AGAAATGATC TCAGAGCAGC AGAGTGAACT GAAGACGCTG 
CGGTCGGAGT TTGACCTGCT GACCTCTGAG CTGCAGCTGA GAGCAGAGCT GACCTCAGAG 
CTGGAAGTCC AAGTTCAGAA GTTGGAGGAG AAAGTTCAGG CTGCAGAGGA GCAGCTGAGC 
ACCGCTTTGG AGGAGAAGAA GAGTCTTTCT GATCAGGTGA CCCAGCTGCT GGAGGAGAGG 
GAGTCACTTA GTCTGCAGCT GGAAACGTCT AAATGTCAAC TCACTGACTT CATGGAGATG 
TTGGAAGGAC TTGAGATGGC CAAAGGCGGA TTGGATGAGA AGTTCCTTCA GCAGGAGAGT 
GAGTTGAAGA GGGTCCGCTC TGAAAAAGCT AACCTGGAGC AGCACATCCT GGGAATGGAG 
TCTGAACTGG AGAGCATGCA AGCAGAAACC TCCAGGCTTA CGGAGGAGCT GGAGATCCAG 
AGGAAGACTT GTTCAGGACG GGAACAACAG ATAGAAACTC TCCTTACGGA GACGACCCAG 
CTGAGAGCTG AACTCGTGTC GTGCTCTGAA GACCGAGATG AGTTGAGTCA GTCTTTAGGT 
CAGTGGAGAG ACAAAGTTCA CGGTTTGGAG AAGACAAACC TGGAAACCAG AAATTTAATC 
TCCATCCTGG AGGAAGACAT CCGGGTCGGA AGAAAGGAGT ACGAAGGTCT GCAAAGCGGC 
ATGGAGAGAG TGAAGGCAGA GAGGGAGCAG TTTTTGCAGC AGGTTGTGGT TCTGGAAGAG 
GCCATTTCTA AACATAACAG GGAGAAGGAG GGGCTGCTCA ACCACCTCCA TGAGATGGAA 
GAAGATCACA CGTCTACCAA CCAAAACACC GAGTCCATGG CCGGCAAAAT CCAGGCGTTG 
GAGGGAGAAG TGTGTCGCCT CTCCCAGTCT CTGGAGTCGT CGCTGCTGGA GAAAGGAGAA 
ATCGCGTCTC GGCTGAACTC CACTCAGGAT GAAGTCCGGC AGATGAGGAC GGGCATCGAG 
AAGCTGCAGG TCCGCATCGA GTCAGACGAG AGGAAGAAGA AGAAGATGGG AGAGCTGCTC 
AAAGCTGCTC AGAGGAAGTC TGACTCGCTG CAGGATCGCA TCGATGCGCT GGAGCGAGAG 
AAAGACGAAT TTGAGCAAAA TCTAGAGGAG GCCGTGTTGC AGGCTGAAGT AGCAAAAGCT 
GAGCTGGAAG AGGAGAGGAC TAAGGTGGAA GAAGAGAAGA AAGAGCTGAA TGAGAAACTG 
ACTGAGCTGT CCGCCGCTCT GGACACTCTG ACATCTCAGA AAGCTCATTT AGAGAGAGAG 
CTGGACATGA AAAATGTAGA GATAGAGGAG CTGAAGGCAG CTAAGGACAA ACTGGAACAG 
GGTTTGGAGA AAGCAGAGGT GGACAGAAGA GAAGAAGACA GACAGAGACA AAGACTAGAG 
GAGCTGGAGA AATGGACGAG AGAGGAAGCA GAGAGACAAA GTGTGCGAGT CGGAGACCTT 
CAGGGCCAGC TGACGGAGTT GGAAAGGGAA AACAACGACC TTAGGGCAAC GTTTGAGTCT 
TTAGGAGAAG AAATAAAGGA TCTTAAAACA CTTGCTCAAG CTAAAGAGGA AGAAATCCTC 
GCTTTGGAGA AGGACAAAGA GAATAAGGCA GAATCCATCT CATTAATCGT AGCAGAGAAG 
AAACGATTAG AGGAAAGAAA TGAACAGCTG GAAAAGGAGA GAGACGACCT TCAGTCTGCT 
CTGGTGTCTG TGAACCAAGA GAAAGCAAAG GCAGATCAGG AGAAAACAGA GTTGGATGAA 
GGGAGAGTAA AGCTTCAGTC CAAGATCTCG TTGGTAGAAA CGGAGAAACA GAACCTCCAA 
CAAACCGTCT CCTTGTTGGA GCAGGAAAAG CTGAGGTCAG AGGCTGAAAC TGAGGAGTTG 
CAGTCTTCAC TGTTGATGAT GGAAGAGGAG AAGAACAACC TGTCTTCAGC TCTTTCTCTG 
CTGGAAGAAG AGAAGCAGCA GGCAACAGAG GAGAAAGAGA GGCTCACACA GGAACATGAG 
GTTCTCCAGA AAACAGTCGC CTCCATGGAA GAAGAACTGG AGACACACAA ACGATCCATG 
ACCCAGCTCA GTGAGCAGGT TTCTGAACTA ACATCCAGTT TAGCTCGTCT GACAAAAGAG 
AGAGACTCTG CTCTGAGCAA AATGAACCTT TGGATGAAGA CCTGCAAACA GCTGGAGCAG 
GAGAAGCAGA ACATCCTAAA CAGCTCAGAA GGAAGAAGCA GTGAGGAGTT TCAGGCTGAA 
GCGGCCCAGC TGAGGGCGCA GGCAGAGGTC AGGAAGAAAG AAGTCCAAGA ACTGAAAACC 
GCCCTGGAGA AAAAAACGAC AGAGGCTGAG GAAAGACGAC AAGAATTGAA GCAAAAGGAG 
GAAGAGGTGA AGGAGCGAGC GGCTGCTCTG GAGGGAGTGA GAGCGGAGAA GGAAAGGGAG 
CTGGAGGAGA TCAGGAAGGA ACTGGATGAG GTTAACGCGC TTCTGGAGGA GAAAAGCACC 
GAGGCCGATG AGAGCATAGA GAAATACTGC AGCCTGCTGG TCAAAGTCCA CAAGCTGGAG 
GAGAGCAATG ACGCGCTGAA GACCCGACTG GAGCATCTCA CTGCCAGCCA GCCTGTGAAT 
GAAGCCAAGG TCCCTCCTGA AACCCGCCGG CGTTCAGTGA GGAAGTCTTC CTCCAAACAC 
CAGGAAGAAA AAATGAGTGA AAACACAGAG AATCTGGTTC CCACGACAAC ACCGAGCTCT 
CCACAGGGAG GGTCTCCGGG GAAACGGGGT CATAAAGAAA TCAGTGACAG AGACAGCGCC 
CAGGAGGCTC TGCACAACCT GACGAAGAAA CTAAAAGCCA ACACCGCGAC GCCCAGAGGA 
AGAGGAGAAC AGGATGACGA AGAGTTCAGA CCAGAGGGCC TGCCTGACCT GGTGCAGAAA 
GGGTTCGCTG ATATCCCACT GGGGGAGGCC AGTCCGTACA TCATCCGGAG GACCACCGGG 
AGGCGCTGCA GTCCTCGCCT GGCTGCAAGG CAGACTAAGC CTGACATCAA GGTTTTGGGG 
CCCGTCCATC TGCAGAGTCC CTCTGGTTCA TCATCGGACG GCTCCGACAG GACGCGCCAG 
CCTCTGAGTG GCGAACAGGA ACCTTCGCTG AATGTCCATC AGGAAGCAGA GCAGAGAGAG 
AAACAGGTTT TAGTGGAGCA AACAAAACAA GGAGAAAACT GTCATGTTCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023188117.1
CDS	53..8266
Translation	MSWAVEEWKDGLPGKALQKIQEMEVQLDKLKKERTQKQFQLDSLEAALQKQKQKVDSERTEISALKRENQSMIESCDALEKTRQKVVHDLGVKEQQVSYLEGQLNSCRKTIDRLEQELKKYKTELDRSQPAGSSLLSTSSSDLQTFSTPQKTFATPAPVAAHRQPDSKLDELQEKYNHEVEERRKLESELKMLQVKLLNQSSVSHKDIAARQAGSSIFPWQQQDQVHSLRGQDAMETPLKRRGASLWDTHEETPIKPNQRMSSSRTVQSPSGSTQQTEQLKSLNQELRGRVSELERNLAAQEKEIRNQTSKLQELQTHLNQTRKELAERDRELAKAGHELSQAADRHQQLEAKCSLVEQKLKQVSEEMSCQRHNAESCRRALEQKLKDQERNSQKELAQLQSSHQALDQQLNQTRTKLTQEIQQAKKDHNVLQADMEKTRLQKSQMEREMEEQKQKLLRSEQGLQATQTKEQDLRKKMEELQKEKNGVTIQLDRCSRSLTQLEEEKRISEQSLKRTQGLLEDLKAKSEGQAEELKRLQSKFEQQTQTAARELEHVKKTLSDAEARNDKSQNELQKQKQESEKLSNKLTVIEQESEELKSNLRQSRDECQAVKQEHQALLEWKKNKENLINQTEAAQQELTDKIITLEGKASRMHEANNELQDKISSTEADKASLSAHIDALKGELLMRSTELEEKEHQYQQLQLHVSEAAQKHGKDLENAGKQLAQLQGQVKELESRLQREASRAELAEKTNCELQEEHQAACDLLRSKDQLLELGRAEVSQLKDSLAQSAAQQEAQSDSFVKQKAVLLKRCEESISAQAEETERVKLQAEEVQQELLLSRHKITSMEQILKVQEQLGAELQKQIKTTSDKEEELTKTCEEKSEELKRLEEELKDQRSLTEETEQQIRAAEARIHSVENQKAELENRLQEMKTEAEILQFSHTERMNALQEQIVCLEKQVAANQEEAEEVPVLKNKLDVVNQSLAHLNKSLEASEKSLSCANEIKADLEVTLSEKVKLITALEDQINNLTEKLRKESESHSSEVEDFINKERSLKEQLEATRKSVAAAKEESSSRRENIREMKMTLSAASRGLEERDNTIKSLKEKLNKAEAEQAKASELLKEKTVAMSKIKVQLEMLQMDLEDNELAMTSVGSQAEELKGTIASLEAMLEESKAQVSVLETRLDEVQYQNSLLETKYVTAKDELLERSCEITRLEEDAVRRQQLEENVSALEAKLSQITEENSKAETELRQIIQEKESHLEQVNEERNSLQATLTQSVDEQKRAESEITQVKAVKAELEERMSNLSKEIQQLHADVRELSEQKQEAEGTIDQMAAETQQTESALRRISEEKAQLDVALSSVSEEKVELQERISTLTSENGELVCKVKQVEEEKLQLESKFSQISEENVKQQSDMNRLTAEKQQLQSSAERLQEEMASLREESERVQSSLLSAEEERQMLREQLNTRDETNHTESRARNQQFEAEQTALHQKLSILQTELAVLQQQYDLLLQQVDQQQRIIQQLTESQKYQNPTGNIPRLEPKDAEDGEAAEQTKPNPELGANVGTSAESDPLNEQEEASEKSPDQAASEAELIVREDASDQEMINEEEAHKHDAQEIQLRQQVSEENCSSSGDLENEKYDASSFQHEIRGMKFSGDESWPVKDVDTEMISEQQSELKTLRSEFDLLTSELQLRAELTSELEVQVQKLEEKVQAAEEQLSTALEEKKSLSDQVTQLLEERESLSLQLETSKCQLTDFMEMLEGLEMAKGGLDEKFLQQESELKRVRSEKANLEQHILGMESELESMQAETSRLTEELEIQRKTCSGREQQIETLLTETTQLRAELVSCSEDRDELSQSLGQWRDKVHGLEKTNLETRNLISILEEDIRVGRKEYEGLQSGMERVKAEREQFLQQVVVLEEAISKHNREKEGLLNHLHEMEEDHTSTNQNTESMAGKIQALEGEVCRLSQSLESSLLEKGEIASRLNSTQDEVRQMRTGIEKLQVRIESDERKKKKMGELLKAAQRKSDSLQDRIDALEREKDEFEQNLEEAVLQAEVAKAELEEERTKVEEEKKELNEKLTELSAALDTLTSQKAHLERELDMKNVEIEELKAAKDKLEQGLEKAEVDRREEDRQRQRLEELEKWTREEAERQSVRVGDLQGQLTELERENNDLRATFESLGEEIKDLKTLAQAKEEEILALEKDKENKAESISLIVAEKKRLEERNEQLEKERDDLQSALVSVNQEKAKADQEKTELDEGRVKLQSKISLVETEKQNLQQTVSLLEQEKLRSEAETEELQSSLLMMEEEKNNLSSALSLLEEEKQQATEEKERLTQEHEVLQKTVASMEEELETHKRSMTQLSEQVSELTSSLARLTKERDSALSKMNLWMKTCKQLEQEKQNILNSSEGRSSEEFQAEAAQLRAQAEVRKKEVQELKTALEKKTTEAEERRQELKQKEEEVKERAAALEGVRAEKERELEEIRKELDEVNALLEEKSTEADESIEKYCSLLVKVHKLEESNDALKTRLEHLTASQPVNEAKVPPETRRRSVRKSSSKHQEEKMSENTENLVPTTTPSSPQGGSPGKRGHKEISDRDSAQEALHNLTKKLKANTATPRGRGEQDDEEFRPEGLPDLVQKGFADIPLGEASPYIIRRTTGRRCSPRLAARQTKPDIKVLGPVHLQSPSGSSSDGSDRTRQPLSGEQEPSLNVHQEAEQREKQVLVEQTKQGENCHVQ

Target ORF information:

RefSeq Version	XM_023332349.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus centromere protein F (cenpf), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023332349.1

ORF Insert Sequence:

ATGAGCTGGG CGGTGGAGGA GTGGAAGGAT GGACTTCCAG GGAAAGCCCT GCAGAAGATC 
CAGGAGATGG AGGTCCAACT GGACAAACTG AAGAAGGAAA GGACACAGAA GCAGTTTCAG 
CTGGACTCTC TGGAAGCTGC CCTGCAGAAA CAGAAGCAGA AGGTGGACAG TGAACGCACC 
GAGATCTCCG CTTTGAAAAG AGAAAACCAG TCGATGATTG AGTCATGTGA CGCTTTGGAG 
AAAACTCGTC AGAAGGTGGT TCATGACCTT GGAGTCAAAG AGCAGCAGGT GAGCTACCTG 
GAGGGTCAAC TTAACTCCTG CAGGAAGACA ATAGATCGTC TGGAGCAAGA ACTCAAGAAG 
TACAAAACTG AGCTCGATCG CTCTCAACCT GCCGGATCTT CCTTGCTGTC CACCTCTTCC 
TCTGACCTGC AGACGTTCAG CACGCCACAG AAGACTTTTG CTACGCCAGC GCCTGTCGCT 
GCACACAGAC AGCCAGACAG CAAACTGGAC GAGCTTCAGG AAAAGTACAA CCATGAAGTG 
GAAGAAAGAA GAAAGCTGGA AAGTGAGCTC AAAATGTTGC AGGTCAAGTT GCTGAATCAG 
TCATCCGTCA GCCACAAGGA CATCGCCGCC CGCCAAGCTG GGTCATCCAT ATTCCCATGG 
CAACAGCAGG ATCAGGTCCA CAGCCTACGG GGCCAGGATG CGATGGAAAC GCCACTAAAG 
AGACGCGGTG CTTCTCTGTG GGACACCCAC GAGGAAACGC CGATCAAACC CAACCAGCGG 
ATGAGTTCGT CCAGAACCGT CCAGAGTCCC AGTGGATCGA CCCAGCAGAC GGAGCAGCTG 
AAGAGCCTCA ACCAGGAGCT GCGTGGACGT GTGTCAGAGC TGGAGAGGAA TCTGGCTGCT 
CAGGAAAAGG AAATTCGTAA CCAAACATCG AAGCTGCAAG AACTCCAAAC TCACTTGAAC 
CAAACCCGCA AAGAGCTGGC GGAGCGGGAC AGAGAGCTGG CCAAGGCCGG CCACGAGCTG 
AGCCAGGCTG CAGACCGCCA CCAGCAGCTG GAGGCAAAGT GCTCCTTGGT GGAGCAGAAG 
CTGAAGCAGG TCTCCGAGGA GATGAGCTGC CAGAGGCACA ACGCTGAAAG CTGCAGACGA 
GCTCTGGAGC AGAAACTCAA AGACCAAGAA AGAAACAGTC AGAAGGAGTT GGCTCAGCTT 
CAGAGTTCCC ATCAGGCTTT GGATCAGCAG CTCAACCAGA CCAGAACCAA GCTGACACAG 
GAGATCCAAC AGGCCAAAAA AGACCACAAT GTTCTTCAAG CGGACATGGA GAAGACGCGC 
TTGCAGAAGA GTCAGATGGA GAGAGAAATG GAGGAGCAGA AGCAGAAGCT GCTGCGGTCC 
GAACAAGGCC TGCAGGCGAC TCAGACCAAA GAGCAAGACC TCCGCAAAAA GATGGAGGAG 
CTGCAGAAAG AGAAGAACGG CGTGACCATC CAGTTGGACC GCTGCAGCAG GTCGCTGACT 
CAGCTGGAAG AGGAGAAGAG GATCTCAGAG CAGTCGCTGA AACGCACCCA GGGGCTGCTG 
GAGGACCTGA AAGCGAAATC TGAAGGACAG GCCGAGGAGC TGAAGAGACT CCAGTCAAAA 
TTTGAGCAAC AGACTCAGAC GGCAGCAAGA GAGCTGGAAC ACGTGAAGAA GACGCTTTCT 
GACGCAGAGG CCAGGAATGA CAAATCTCAG AATGAGCTTC AAAAGCAGAA GCAGGAGAGT 
GAGAAGCTGA GCAACAAGCT GACGGTCATA GAGCAGGAGA GCGAAGAGCT GAAATCAAAC 
CTCCGTCAGA GTCGGGACGA GTGCCAGGCT GTGAAACAGG AGCACCAGGC TCTGCTGGAG 
TGGAAGAAAA ACAAGGAGAA CTTGATAAAC CAAACCGAGG CCGCGCAGCA GGAGCTCACT 
GACAAGATTA TCACTTTGGA GGGCAAAGCG AGCCGGATGC ATGAGGCCAA CAATGAACTC 
CAGGACAAGA TCTCATCCAC GGAGGCAGAC AAAGCCAGTC TGTCCGCCCA CATCGACGCC 
CTGAAGGGAG AACTCCTCAT GAGGAGCACG GAGCTGGAGG AGAAGGAGCA TCAGTACCAG 
CAGCTTCAGC TCCACGTCTC TGAAGCGGCA CAGAAACACG GCAAAGACCT GGAGAACGCC 
GGGAAGCAGC TGGCGCAGCT CCAAGGACAG GTGAAAGAGC TGGAGTCCCG GCTGCAGAGG 
GAGGCGTCTC GAGCAGAGCT GGCCGAAAAG ACGAACTGCG AGCTGCAGGA GGAGCACCAG 
GCCGCGTGCG ACCTGCTGCG CTCCAAAGAC CAGCTGCTGG AGCTGGGCCG GGCCGAGGTC 
AGCCAGCTGA AGGACAGCCT CGCTCAGTCC GCCGCACAGC AGGAGGCGCA GAGCGACAGC 
TTTGTGAAGC AGAAGGCCGT CCTGCTGAAG CGGTGTGAGG AGAGCATTTC GGCGCAGGCT 
GAGGAGACGG AGCGCGTCAA GCTGCAGGCA GAGGAAGTCC AGCAGGAGCT GCTGCTCTCC 
AGACACAAAA TCACCTCGAT GGAGCAGATC CTGAAGGTCC AGGAACAGCT GGGAGCCGAG 
CTGCAGAAAC AAATTAAGAC GACGTCCGAT AAGGAGGAGG AGCTCACGAA GACGTGCGAG 
GAGAAATCAG AGGAGCTGAA ACGCTTGGAG GAGGAGCTGA AGGACCAGCG GAGCCTCACC 
GAGGAGACGG AGCAGCAGAT CAGAGCGGCT GAAGCTCGGA TCCACTCCGT GGAAAATCAA 
AAGGCTGAGC TGGAAAATCG ACTTCAAGAG ATGAAGACAG AAGCAGAGAT CCTGCAGTTC 
AGTCACACTG AAAGGATGAA CGCTCTCCAG GAACAGATCG TATGTCTGGA AAAGCAGGTT 
GCTGCAAACC AGGAAGAAGC AGAGGAGGTT CCTGTCCTGA AAAACAAACT GGACGTGGTC 
AACCAGTCTC TTGCTCACCT TAACAAATCT CTTGAAGCTT CAGAAAAGAG CCTCAGCTGT 
GCAAATGAAA TAAAAGCTGA TCTGGAGGTC ACCCTGTCTG AGAAAGTGAA ACTTATCACT 
GCTTTGGAGG ATCAGATCAA TAATCTCACT GAGAAGCTGA GGAAAGAGTC TGAGAGCCAC 
AGCTCAGAGG TTGAGGACTT CATCAATAAG GAGAGGAGCC TCAAAGAGCA GCTGGAAGCC 
ACTAGGAAAT CAGTAGCTGC AGCCAAAGAA GAGTCCAGCT CTAGACGGGA GAATATCCGA 
GAAATGAAGA TGACTCTGTC TGCAGCGTCC CGCGGCCTGG AAGAAAGAGA CAACACGATA 
AAGAGTCTGA AAGAGAAGCT GAACAAAGCA GAGGCAGAGC AGGCCAAAGC CTCTGAGCTC 
CTGAAGGAGA AGACGGTGGC CATGAGCAAA ATCAAGGTGC AGCTGGAGAT GCTGCAGATG 
GACCTGGAGG ACAACGAGTT GGCCATGACC TCTGTGGGCA GTCAGGCGGA GGAGCTGAAG 
GGAACCATAG CGTCCCTGGA GGCCATGCTG GAGGAAAGCA AAGCCCAGGT TTCTGTTTTG 
GAGACCCGTT TGGATGAGGT CCAGTACCAA AACTCTCTGC TGGAGACAAA ATACGTGACG 
GCGAAGGACG AGCTGCTGGA GCGGAGCTGC GAGATAACCC GTCTGGAGGA GGACGCCGTC 
AGACGGCAGC AGCTAGAGGA GAACGTCTCT GCCTTGGAGG CCAAACTGAG TCAGATTACT 
GAGGAAAACT CAAAGGCAGA GACGGAGCTG AGGCAGATCA TTCAGGAGAA AGAGAGCCAT 
TTGGAGCAAG TAAACGAGGA GAGAAACAGC CTCCAGGCTA CCTTAACGCA GTCAGTGGAT 
GAGCAGAAAC GAGCTGAATC TGAAATCACT CAGGTAAAAG CGGTGAAGGC GGAGCTGGAG 
GAGAGAATGA GCAACCTGTC GAAGGAGATT CAGCAACTCC ATGCTGATGT TAGAGAGTTA 
TCTGAACAGA AACAGGAAGC GGAGGGAACG ATTGACCAGA TGGCTGCAGA AACACAACAG 
ACAGAGTCCG CTCTCCGTCG GATCTCAGAG GAGAAAGCCC AGCTGGACGT TGCTCTGAGT 
TCAGTCAGCG AGGAAAAAGT TGAGCTGCAG GAGAGGATTT CTACTTTAAC CTCTGAGAAC 
GGGGAGCTGG TCTGCAAAGT GAAGCAGGTG GAGGAGGAGA AACTCCAGCT GGAGTCTAAG 
TTTAGTCAAA TCTCTGAGGA AAACGTCAAG CAGCAGTCAG ACATGAACCG TCTGACTGCA 
GAGAAGCAGC AGCTGCAGAG CAGTGCAGAG AGGCTGCAGG AGGAGATGGC GTCTCTGCGG 
GAGGAGAGCG AGCGTGTCCA GAGCTCGCTG CTGTCCGCTG AGGAGGAGCG ACAGATGCTG 
CGGGAGCAGC TCAACACGAG GGACGAGACC AACCACACGG AGAGCAGAGC GCGCAACCAG 
CAGTTTGAGG CGGAGCAGAC TGCACTTCAT CAGAAGCTGT CCATCCTCCA GACGGAGCTG 
GCTGTGCTGC AGCAGCAGTA CGATTTGCTT CTGCAGCAAG TGGACCAGCA GCAACGCATC 
ATTCAGCAGC TCACAGAATC ACAGAAATAC CAGAATCCGA CTGGAAACAT CCCGCGCCTC 
GAACCCAAAG ACGCTGAGGA TGGCGAGGCA GCTGAACAGA CCAAACCAAA CCCAGAACTT 
GGTGCGAACG TCGGTACCAG TGCAGAGTCA GATCCACTGA ATGAACAGGA AGAGGCGTCT 
GAAAAGAGCC CTGATCAGGC TGCGAGTGAG GCAGAGCTGA TCGTTCGGGA GGACGCCTCT 
GATCAGGAGA TGATCAATGA GGAGGAGGCT CATAAACATG ATGCTCAGGA AATCCAACTT 
CGCCAGCAGG TCTCTGAGGA GAACTGCAGC AGTAGTGGAG ATCTTGAAAA TGAGAAGTAT 
GATGCATCTT CCTTCCAACA TGAGATAAGA GGCATGAAAT TTTCTGGAGA CGAGTCTTGG 
CCGGTGAAAG ACGTCGATAC AGAAATGATC TCAGAGCAGC AGAGTGAACT GAAGACGCTG 
CGGTCGGAGT TTGACCTGCT GACCTCTGAG CTGCAGCTGA GAGCAGAGCT GACCTCAGAG 
CTGGAAGTCC AAGTTCAGAA GTTGGAGGAG AAAGTTCAGG CTGCAGAGGA GCAGCTGAGC 
ACCGCTTTGG AGGAGAAGAA GAGTCTTTCT GATCAGGTGA CCCAGCTGCT GGAGGAGAGG 
GAGTCACTTA GTCTGCAGCT GGAAACGTCT AAATGTCAAC TCACTGACTT CATGGAGATG 
TTGGAAGGAC TTGAGATGGC CAAAGGCGGA TTGGATGAGA AGTTCCTTCA GCAGGAGAGT 
GAGTTGAAGA GGGTCCGCTC TGAAAAAGCT AACCTGGAGC AGCACATCCT GGGAATGGAG 
TCTGAACTGG AGAGCATGCA AGCAGAAACC TCCAGGCTTA CGGAGGAGCT GGAGATCCAG 
AGGAAGACTT GTTCAGGACG GGAACAACAG ATAGAAACTC TCCTTACGGA GACGACCCAG 
CTGAGAGCTG AACTCGTGTC GTGCTCTGAA GACCGAGATG AGTTGAGTCA GTCTTTAGGT 
CAGTGGAGAG ACAAAGTTCA CGGTTTGGAG AAGACAAACC TGGAAACCAG AAATTTAATC 
TCCATCCTGG AGGAAGACAT CCGGGTCGGA AGAAAGGAGT ACGAAGGTCT GCAAAGCGGC 
ATGGAGAGAG TGAAGGCAGA GAGGGAGCAG TTTTTGCAGC AGGTTGTGGT TCTGGAAGAG 
GCCATTTCTA AACATAACAG GGAGAAGGAG GGGCTGCTCA ACCACCTCCA TGAGATGGAA 
GAAGATCACA CGTCTACCAA CCAAAACACC GAGTCCATGG CCGGCAAAAT CCAGGCGTTG 
GAGGGAGAAG TGTGTCGCCT CTCCCAGTCT CTGGAGTCGT CGCTGCTGGA GAAAGGAGAA 
ATCGCGTCTC GGCTGAACTC CACTCAGGAT GAAGTCCGGC AGATGAGGAC GGGCATCGAG 
AAGCTGCAGG TCCGCATCGA GTCAGACGAG AGGAAGAAGA AGAAGATGGG AGAGCTGCTC 
AAAGCTGCTC AGAGGAAGTC TGACTCGCTG CAGGATCGCA TCGATGCGCT GGAGCGAGAG 
AAAGACGAAT TTGAGCAAAA TCTAGAGGAG GCCGTGTTGC AGGCTGAAGT AGCAAAAGCT 
GAGCTGGAAG AGGAGAGGAC TAAGGTGGAA GAAGAGAAGA AAGAGCTGAA TGAGAAACTG 
ACTGAGCTGT CCGCCGCTCT GGACACTCTG ACATCTCAGA AAGCTCATTT AGAGAGAGAG 
CTGGACATGA AAAATGTAGA GATAGAGGAG CTGAAGGCAG CTAAGGACAA ACTGGAACAG 
GGTTTGGAGA AAGCAGAGGT GGACAGAAGA GAAGAAGACA GACAGAGACA AAGACTAGAG 
GAGCTGGAGA AATGGACGAG AGAGGAAGCA GAGAGACAAA GTGTGCGAGT CGGAGACCTT 
CAGGGCCAGC TGACGGAGTT GGAAAGGGAA AACAACGACC TTAGGGCAAC GTTTGAGTCT 
TTAGGAGAAG AAATAAAGGA TCTTAAAACA CTTGCTCAAG CTAAAGAGGA AGAAATCCTC 
GCTTTGGAGA AGGACAAAGA GAATAAGGCA GAATCCATCT CATTAATCGT AGCAGAGAAG 
AAACGATTAG AGGAAAGAAA TGAACAGCTG GAAAAGGAGA GAGACGACCT TCAGTCTGCT 
CTGGTGTCTG TGAACCAAGA GAAAGCAAAG GCAGATCAGG AGAAAACAGA GTTGGATGAA 
GGGAGAGTAA AGCTTCAGTC CAAGATCTCG TTGGTAGAAA CGGAGAAACA GAACCTCCAA 
CAAACCGTCT CCTTGTTGGA GCAGGAAAAG CTGAGGTCAG AGGCTGAAAC TGAGGAGTTG 
CAGTCTTCAC TGTTGATGAT GGAAGAGGAG AAGAACAACC TGTCTTCAGC TCTTTCTCTG 
CTGGAAGAAG AGAAGCAGCA GGCAACAGAG GAGAAAGAGA GGCTCACACA GGAACATGAG 
GTTCTCCAGA AAACAGTCGC CTCCATGGAA GAAGAACTGG AGACACACAA ACGATCCATG 
ACCCAGCTCA GTGAGCAGGT TTCTGAACTA ACATCCAGTT TAGCTCGTCT GACAAAAGAG 
AGAGACTCTG CTCTGAGCAA AATGAACCTT TGGATGAAGA CCTGCAAACA GCTGGAGCAG 
GAGAAGCAGA ACATCCTAAA CAGCTCAGAA GGAAGAAGCA GTGAGGAGTT TCAGGCTGAA 
GCGGCCCAGC TGAGGGCGCA GGCAGAGGTC AGGAAGAAAG AAGTCCAAGA ACTGAAAACC 
GCCCTGGAGA AAAAAACGAC AGAGGCTGAG GAAAGACGAC AAGAATTGAA GCAAAAGGAG 
GAAGAGGTGA AGGAGCGAGC GGCTGCTCTG GAGGGAGTGA GAGCGGAGAA GGAAAGGGAG 
CTGGAGGAGA TCAGGAAGGA ACTGGATGAG GTTAACGCGC TTCTGGAGGA GAAAAGCACC 
GAGGCCGATG AGAGCATAGA GAAATACTGC AGCCTGCTGG TCAAAGTCCA CAAGCTGGAG 
GAGAGCAATG ACGCGCTGAA GACCCGACTG GAGCATCTCA CTGCCAGCCA GCCTGTGAAT 
GAAGCCAAGG TCCCTCCTGA AACCCGCCGG CGTTCAGTGA GGAAGTCTTC CTCCAAACAC 
CAGGAAGAAA AAATGAGTGA AAACACAGAG AATCTGGTTC CCACGACAAC ACCGAGCTCT 
CCACAGGGAG GGTCTCCGGG GAAACGGGGT CATAAAGAAA TCAGTGACAG AGACAGCGCC 
CAGGAGGCTC TGCACAACCT GACGAAGAAA CTAAAAGCCA ACACCGCGAC GCCCAGAGGA 
AGAGGAGAAC AGGATGACGA AGAGTTCAGA CCAGAGGGCC TGCCTGACCT GGTGCAGAAA 
GGGTTCGCTG ATATCCCACT GGGGGAGGCC AGTCCGTACA TCATCCGGAG GACCACCGGG 
AGGCGCTGCA GTCCTCGCCT GGCTGCAAGG CAGACTAAGC CTGACATCAA GGTTTTGGGG 
CCCGTCCATC TGCAGAGTCC CTCTGGTTCA TCATCGGACG GCTCCGACAG GACGCGCCAG 
CCTCTGAGTG GCGAACAGGA ACCTTCGCTG AATGTCCATC AGGAAGCAGA GCAGAGAGAG 
AAACAGGTTT TAGTGGAGCA AACAAAACAA GGAGAAAACT GTCATGTTCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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cenpf cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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