mettl27 cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mettl27
Entrez Gene ID	102231724
Full Name	methyltransferase like 27
Synonyms	wbscr27
Gene Type	protein-coding
Organism	Xiphophorus maculatus(southern platyfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005813092.1	XP_005813149.1	Williams-Beuren syndrome chromosomal region 27 protein
XM_005813092.2	XP_005813149.2	methyltransferase-like protein 27 isoform X3
XM_023341627.1	XP_023197395.1	methyltransferase-like protein 27 isoform X2
XM_023341626.1	XP_023197394.1	methyltransferase-like protein 27 isoform X1

Pathways from BioSystems
Gene Ontology

The following mettl27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mettl27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXi18217	XM_005813092.1 Latest version!	Xiphophorus maculatus Williams Beuren syndrome chromosome region 27 (wbscr27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OXi45588	XM_023341626.1 Latest version!	Xiphophorus maculatus methyltransferase like 27 (mettl27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OXi45589	XM_023341627.1 Latest version!	Xiphophorus maculatus methyltransferase like 27 (mettl27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OXi45590	XM_005813092.2 Latest version!	Xiphophorus maculatus methyltransferase like 27 (mettl27), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mettl27 ( XM_005813092.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_005813149.1 Xiphophorus maculatus Williams Beuren syndrome chromosome region 27 (wbscr27), mRNA.

Price & Availability↑

CloneID	OXi18217
Clone ID Related Accession (Same CDS sequence)	XM_005813092.1
Accession Version	XM_005813092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1445702400000
Organism	Xiphophorus maculatus(Southern platyfish)
Product	Williams-Beuren syndrome chromosomal region 27 protein
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005372704.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xiphophorus maculatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTG ACAGTAACAC ATTTGAAAGC GTAAAAGCTG TTATCTTGTC AGCTCATAAA 
AGCACCACAT CAGAAGAGAA GGTCAGCTTC TACAACCGCT GGGCAGAAAA CTACGATCAG 
GATGTGGCCG TTTTGGATTA CCGTGCACCA AGTCTGGCAG CAGACTGCAT CTCGTCCCAT 
TTCAGCAACG TGCGAGAGGC AGCCGTCGTG CTGGATGTGG CATGTGGTAC CGGACTGGTG 
GCCAAACACA TGAAAAAACT CGGGTTTGAA CATTTTGTGG GTGTGGATGG AAGCGAGGCC 
ATGCTGGGCA GGGCGAGAGA GAGCGGCTTG TACCAGGACT TGAAGCAGTG CTTGCTGGGA 
GAGGAGCCTC TCCCCGTACA GTGGGGCAGC TTTGATGTGG TTGTAATTGT TGGAGCGTTA 
AGTGCTGGTC AGGTCCCTAT TGGTATCGCC AGACACTTGT GCAATGCCAC CAAATCAGGT 
GGCTCCATTT GCATGACAAC CAGAAGTAAC CAGGATAACT TGGAGTACAA AGCTGCCCTT 
GACAGCGAGC TGAGGAAGAT GGAGGAAGAG GAGCTCTGGA CTTGTTTAGA GGTCACCGAG 
GTCAAAGACT GGGAGAGGGC TGTATCAGAG AAGGAGGACG GCTACATATC TGGAGTTGTG 
TACCTCTATA AGAAACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005813149.1
CDS	1..681
Translation	MSADSNTFESVKAVILSAHKSTTSEEKVSFYNRWAENYDQDVAVLDYRAPSLAADCISSHFSNVREAAVVLDVACGTGLVAKHMKKLGFEHFVGVDGSEAMLGRARESGLYQDLKQCLLGEEPLPVQWGSFDVVVIVGALSAGQVPIGIARHLCNATKSGGSICMTTRSNQDNLEYKAALDSELRKMEEEELWTCLEVTEVKDWERAVSEKEDGYISGVVYLYKKL

Target ORF information:

RefSeq Version	XM_005813092.1
Organism	Xiphophorus maculatus(Southern platyfish)
Definition	Xiphophorus maculatus Williams Beuren syndrome chromosome region 27 (wbscr27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005813092.1

ORF Insert Sequence:

ATGTCTGCTG ACAGTAACAC ATTTGAAAGC GTAAAAGCTG TTATCTTGTC AGCTCATAAA 
AGCACCACAT CAGAAGAGAA GGTCAGCTTC TACAACCGCT GGGCAGAAAA CTACGATCAG 
GATGTGGCCG TTTTGGATTA CCGTGCACCA AGTCTGGCAG CAGACTGCAT CTCGTCCCAT 
TTCAGCAACG TGCGAGAGGC AGCCGTCGTG CTGGATGTGG CATGTGGTAC CGGACTGGTG 
GCCAAACACA TGAAAAAACT CGGGTTTGAA CATTTTGTGG GTGTGGATGG AAGCGAGGCC 
ATGCTGGGCA GGGCGAGAGA GAGCGGCTTG TACCAGGACT TGAAGCAGTG CTTGCTGGGA 
GAGGAGCCTC TCCCCGTACA GTGGGGCAGC TTTGATGTGG TTGTAATTGT TGGAGCGTTA 
AGTGCTGGTC AGGTCCCTAT TGGTATCGCC AGACACTTGT GCAATGCCAC CAAATCAGGT 
GGCTCCATTT GCATGACAAC CAGAAGTAAC CAGGATAACT TGGAGTACAA AGCTGCCCTT 
GACAGCGAGC TGAGGAAGAT GGAGGAAGAG GAGCTCTGGA CTTGTTTAGA GGTCACCGAG 
GTCAAAGACT GGGAGAGGGC TGTATCAGAG AAGGAGGACG GCTACATATC TGGAGTTGTG 
TACCTCTATA AGAAACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mettl27 ( XM_023341626.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023197394.1 Xiphophorus maculatus methyltransferase like 27 (mettl27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXi45588
Clone ID Related Accession (Same CDS sequence)	XM_023341626.1
Accession Version	XM_023341626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	methyltransferase-like protein 27 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036453.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGGTT TTTGTATAAT GTCTGCTGAC AGTAACACAT TTGAAAGCGT AAAAGCTGTT 
ATCTTGTCAG CTCATAAAAG CACCACATCA GAAGAGAAGG TCAGCTTCTA CAACCGCTGG 
GCAGAAAACT ACGATCAGTT GCTACATGTG AAAGACATTT CCTCAGATGT GGCCGTTTTG 
GATTACCGTG CACCAAGTCT GGCAGCAGAC TGCATCTCGT CCCATTTCAG CAACGTGCGA 
GAGGCAGCCG TCGTGCTGGA TGTGGCATGT GGTACCGGAC TGGTGGCCAA ACACATGAAA 
AAACTCGGGT TTGAACATTT TGTGGGTGTG GATGGAAGCG AGGCCATGCT GGGCAGGGCG 
AGAGAGAGCG GCTTGTACCA GGACTTGAAG CAGTGCTTGC TGGGAGAGGA GCCTCTCCCC 
GTACAGTGGG GCAGCTTTGA TGTGGTTGTA ATTGTTGGAG CGTTAAGTGC TGGTCAGGTC 
CCTATTGGTA TCGCCAGACA CTTGTGCAAT GCCACCAAAT CAGGTGGCTC CATTTGCATG 
ACAACCAGAA GTAACCAGGA TAACTTGGAG TACAAAGCTG CCCTTGACAG CGAGCTGAGG 
AAGATGGAGG AAGAGGAGCT CTGGACTTGT TTAGAGGTCA CCGAGGTCAA AGACTGGGAG 
AGGGCTGTAT CAGAGAAGGA GGACGGCTAC ATATCTGGAG TTGTGTACCT CTATAAGAAA 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023197394.1
CDS	61..786
Translation	MTGFCIMSADSNTFESVKAVILSAHKSTTSEEKVSFYNRWAENYDQLLHVKDISSDVAVLDYRAPSLAADCISSHFSNVREAAVVLDVACGTGLVAKHMKKLGFEHFVGVDGSEAMLGRARESGLYQDLKQCLLGEEPLPVQWGSFDVVVIVGALSAGQVPIGIARHLCNATKSGGSICMTTRSNQDNLEYKAALDSELRKMEEEELWTCLEVTEVKDWERAVSEKEDGYISGVVYLYKKL

Target ORF information:

RefSeq Version	XM_023341626.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus methyltransferase like 27 (mettl27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023341626.1

ORF Insert Sequence:

ATGACAGGTT TTTGTATAAT GTCTGCTGAC AGTAACACAT TTGAAAGCGT AAAAGCTGTT 
ATCTTGTCAG CTCATAAAAG CACCACATCA GAAGAGAAGG TCAGCTTCTA CAACCGCTGG 
GCAGAAAACT ACGATCAGTT GCTACATGTG AAAGACATTT CCTCAGATGT GGCCGTTTTG 
GATTACCGTG CACCAAGTCT GGCAGCAGAC TGCATCTCGT CCCATTTCAG CAACGTGCGA 
GAGGCAGCCG TCGTGCTGGA TGTGGCATGT GGTACCGGAC TGGTGGCCAA ACACATGAAA 
AAACTCGGGT TTGAACATTT TGTGGGTGTG GATGGAAGCG AGGCCATGCT GGGCAGGGCG 
AGAGAGAGCG GCTTGTACCA GGACTTGAAG CAGTGCTTGC TGGGAGAGGA GCCTCTCCCC 
GTACAGTGGG GCAGCTTTGA TGTGGTTGTA ATTGTTGGAG CGTTAAGTGC TGGTCAGGTC 
CCTATTGGTA TCGCCAGACA CTTGTGCAAT GCCACCAAAT CAGGTGGCTC CATTTGCATG 
ACAACCAGAA GTAACCAGGA TAACTTGGAG TACAAAGCTG CCCTTGACAG CGAGCTGAGG 
AAGATGGAGG AAGAGGAGCT CTGGACTTGT TTAGAGGTCA CCGAGGTCAA AGACTGGGAG 
AGGGCTGTAT CAGAGAAGGA GGACGGCTAC ATATCTGGAG TTGTGTACCT CTATAAGAAA 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mettl27 ( XM_023341627.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023197395.1 Xiphophorus maculatus methyltransferase like 27 (mettl27), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXi45589
Clone ID Related Accession (Same CDS sequence)	XM_023341627.1
Accession Version	XM_023341627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	methyltransferase-like protein 27 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036453.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTG ACAGTAACAC ATTTGAAAGC GTAAAAGCTG TTATCTTGTC AGCTCATAAA 
AGCACCACAT CAGAAGAGAA GGTCAGCTTC TACAACCGCT GGGCAGAAAA CTACGATCAG 
TTGCTACATG TGAAAGACAT TTCCTCAGAT GTGGCCGTTT TGGATTACCG TGCACCAAGT 
CTGGCAGCAG ACTGCATCTC GTCCCATTTC AGCAACGTGC GAGAGGCAGC CGTCGTGCTG 
GATGTGGCAT GTGGTACCGG ACTGGTGGCC AAACACATGA AAAAACTCGG GTTTGAACAT 
TTTGTGGGTG TGGATGGAAG CGAGGCCATG CTGGGCAGGG CGAGAGAGAG CGGCTTGTAC 
CAGGACTTGA AGCAGTGCTT GCTGGGAGAG GAGCCTCTCC CCGTACAGTG GGGCAGCTTT 
GATGTGGTTG TAATTGTTGG AGCGTTAAGT GCTGGTCAGG TCCCTATTGG TATCGCCAGA 
CACTTGTGCA ATGCCACCAA ATCAGGTGGC TCCATTTGCA TGACAACCAG AAGTAACCAG 
GATAACTTGG AGTACAAAGC TGCCCTTGAC AGCGAGCTGA GGAAGATGGA GGAAGAGGAG 
CTCTGGACTT GTTTAGAGGT CACCGAGGTC AAAGACTGGG AGAGGGCTGT ATCAGAGAAG 
GAGGACGGCT ACATATCTGG AGTTGTGTAC CTCTATAAGA AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023197395.1
CDS	69..776
Translation	MSADSNTFESVKAVILSAHKSTTSEEKVSFYNRWAENYDQLLHVKDISSDVAVLDYRAPSLAADCISSHFSNVREAAVVLDVACGTGLVAKHMKKLGFEHFVGVDGSEAMLGRARESGLYQDLKQCLLGEEPLPVQWGSFDVVVIVGALSAGQVPIGIARHLCNATKSGGSICMTTRSNQDNLEYKAALDSELRKMEEEELWTCLEVTEVKDWERAVSEKEDGYISGVVYLYKKL

Target ORF information:

RefSeq Version	XM_023341627.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus methyltransferase like 27 (mettl27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023341627.1

ORF Insert Sequence:

ATGTCTGCTG ACAGTAACAC ATTTGAAAGC GTAAAAGCTG TTATCTTGTC AGCTCATAAA 
AGCACCACAT CAGAAGAGAA GGTCAGCTTC TACAACCGCT GGGCAGAAAA CTACGATCAG 
TTGCTACATG TGAAAGACAT TTCCTCAGAT GTGGCCGTTT TGGATTACCG TGCACCAAGT 
CTGGCAGCAG ACTGCATCTC GTCCCATTTC AGCAACGTGC GAGAGGCAGC CGTCGTGCTG 
GATGTGGCAT GTGGTACCGG ACTGGTGGCC AAACACATGA AAAAACTCGG GTTTGAACAT 
TTTGTGGGTG TGGATGGAAG CGAGGCCATG CTGGGCAGGG CGAGAGAGAG CGGCTTGTAC 
CAGGACTTGA AGCAGTGCTT GCTGGGAGAG GAGCCTCTCC CCGTACAGTG GGGCAGCTTT 
GATGTGGTTG TAATTGTTGG AGCGTTAAGT GCTGGTCAGG TCCCTATTGG TATCGCCAGA 
CACTTGTGCA ATGCCACCAA ATCAGGTGGC TCCATTTGCA TGACAACCAG AAGTAACCAG 
GATAACTTGG AGTACAAAGC TGCCCTTGAC AGCGAGCTGA GGAAGATGGA GGAAGAGGAG 
CTCTGGACTT GTTTAGAGGT CACCGAGGTC AAAGACTGGG AGAGGGCTGT ATCAGAGAAG 
GAGGACGGCT ACATATCTGG AGTTGTGTAC CTCTATAAGA AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mettl27 ( XM_005813092.2 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_005813149.2 Xiphophorus maculatus methyltransferase like 27 (mettl27), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXi45590
Clone ID Related Accession (Same CDS sequence)	XM_005813092.2
Accession Version	XM_005813092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	methyltransferase-like protein 27 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036453.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 22, 2017 this sequence version replaced XM_005813092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGGTT TTTGTATAAT GTCTGCTGAC AGTAACACAT TTGAAAGCGT AAAAGCTGTT 
ATCTTGTCAG CTCATAAAAG CACCACATCA GAAGAGAAGG TCAGCTTCTA CAACCGCTGG 
GCAGAAAACT ACGATCAGGA TGTGGCCGTT TTGGATTACC GTGCACCAAG TCTGGCAGCA 
GACTGCATCT CGTCCCATTT CAGCAACGTG CGAGAGGCAG CCGTCGTGCT GGATGTGGCA 
TGTGGTACCG GACTGGTGGC CAAACACATG AAAAAACTCG GGTTTGAACA TTTTGTGGGT 
GTGGATGGAA GCGAGGCCAT GCTGGGCAGG GCGAGAGAGA GCGGCTTGTA CCAGGACTTG 
AAGCAGTGCT TGCTGGGAGA GGAGCCTCTC CCCGTACAGT GGGGCAGCTT TGATGTGGTT 
GTAATTGTTG GAGCGTTAAG TGCTGGTCAG GTCCCTATTG GTATCGCCAG ACACTTGTGC 
AATGCCACCA AATCAGGTGG CTCCATTTGC ATGACAACCA GAAGTAACCA GGATAACTTG 
GAGTACAAAG CTGCCCTTGA CAGCGAGCTG AGGAAGATGG AGGAAGAGGA GCTCTGGACT 
TGTTTAGAGG TCACCGAGGT CAAAGACTGG GAGAGGGCTG TATCAGAGAA GGAGGACGGC 
TACATATCTG GAGTTGTGTA CCTCTATAAG AAACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005813149.2
CDS	61..759
Translation	MTGFCIMSADSNTFESVKAVILSAHKSTTSEEKVSFYNRWAENYDQDVAVLDYRAPSLAADCISSHFSNVREAAVVLDVACGTGLVAKHMKKLGFEHFVGVDGSEAMLGRARESGLYQDLKQCLLGEEPLPVQWGSFDVVVIVGALSAGQVPIGIARHLCNATKSGGSICMTTRSNQDNLEYKAALDSELRKMEEEELWTCLEVTEVKDWERAVSEKEDGYISGVVYLYKKL

Target ORF information:

RefSeq Version	XM_005813092.2
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus methyltransferase like 27 (mettl27), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005813092.2

ORF Insert Sequence:

ATGACAGGTT TTTGTATAAT GTCTGCTGAC AGTAACACAT TTGAAAGCGT AAAAGCTGTT 
ATCTTGTCAG CTCATAAAAG CACCACATCA GAAGAGAAGG TCAGCTTCTA CAACCGCTGG 
GCAGAAAACT ACGATCAGGA TGTGGCCGTT TTGGATTACC GTGCACCAAG TCTGGCAGCA 
GACTGCATCT CGTCCCATTT CAGCAACGTG CGAGAGGCAG CCGTCGTGCT GGATGTGGCA 
TGTGGTACCG GACTGGTGGC CAAACACATG AAAAAACTCG GGTTTGAACA TTTTGTGGGT 
GTGGATGGAA GCGAGGCCAT GCTGGGCAGG GCGAGAGAGA GCGGCTTGTA CCAGGACTTG 
AAGCAGTGCT TGCTGGGAGA GGAGCCTCTC CCCGTACAGT GGGGCAGCTT TGATGTGGTT 
GTAATTGTTG GAGCGTTAAG TGCTGGTCAG GTCCCTATTG GTATCGCCAG ACACTTGTGC 
AATGCCACCA AATCAGGTGG CTCCATTTGC ATGACAACCA GAAGTAACCA GGATAACTTG 
GAGTACAAAG CTGCCCTTGA CAGCGAGCTG AGGAAGATGG AGGAAGAGGA GCTCTGGACT 
TGTTTAGAGG TCACCGAGGT CAAAGACTGG GAGAGGGCTG TATCAGAGAA GGAGGACGGC 
TACATATCTG GAGTTGTGTA CCTCTATAAG AAACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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mettl27 cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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