agl cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	agl
Entrez Gene ID	102230673
Full Name	amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
Gene Type	protein-coding
Organism	Xiphophorus maculatus(southern platyfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023334840.1	XP_023190608.1	glycogen debranching enzyme isoform X2
XM_023334838.1	XP_023190606.1	glycogen debranching enzyme isoform X1
XM_023334841.1	XP_023190609.1	glycogen debranching enzyme isoform X3
XM_023334842.1	XP_023190610.1	glycogen debranching enzyme isoform X4
XM_014472572.1	XP_014328058.1	glycogen debranching enzyme
XM_023334839.1	XP_023190607.1	glycogen debranching enzyme isoform X2

Pathways from BioSystems
Gene Ontology

The following agl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the agl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXi39001	XM_023334842.1 Latest version!	Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXi14483	XM_014472572.1 Latest version!	Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXi38998	XM_023334838.1 Latest version!	Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXi38999	XM_023334840.1 Latest version!	Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXi38999	XM_023334839.1 Latest version!	Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXi39000	XM_023334841.1 Latest version!	Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

agl ( XM_023334842.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023190610.1 Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXi39001
Clone ID Related Accession (Same CDS sequence)	XM_023334842.1
Accession Version	XM_023334842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	glycogen debranching enzyme isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036448.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTACA ACATGATCCA CTTCACTCCT CTGCAGACTT TGGGAGAGTC TAGGTCCTGT 
TACTCCTTAG CTGACCAGCT GACCTTTAGC CTGGAGTTCA GCCCAGATGG CAAGAGTTGC 
ACCTGGGAAG ATGTGGGAAG ACTGGTGGAA AAACTGAGGA TGGAATGGAA TATGGTATCA 
ATCACTGATG TGGTGTATAA TCACACTGCT GCAAACAGTG TGTGGATAAA GGAACATCCA 
GAGTGTGGCT ACAACCTGAT GAACTCTCCT CATCTGCGTC CTGCCTGGGT CTTGGACAGA 
GCTATCTGGC ATTTAACCAC TAGGGTAGCA GATGGACGCT ACAAAGAGAA GGGACTTCCT 
TCTGACATTA CCAACGAGTG CCACCTAAAT GCTCTCCGCA GGGTGTTTTG GGAGGACATG 
TTTCCTCAAA TCAAACTTTG GGAGTTCTAC CAGGTCAACG TGGAAAATGC TGTGGAGCAG 
TTCAGAATCC TGTTGCAAAA TGGCACAAAG CCAGATCACA GCAAGACTGA AGGAAATAAA 
GGATTAGAGA TCATTCAAGA CCTCCAGTGC TGTCGCTATG GCAACAGAGT AAACATGGAC 
TCAGCTTTGG AGACATTTGT ACCTCACAGC TCCTCTCCAC AGCACATTGA AGAGTGCTGT 
GGTTGGCTGA AACAGAAGTT GACTGAGATG AATGAAGAAC AGTACCACAT CATACACCAG 
CATCAGGAAC AGGCAGTCAA CTGCATTGTG GAGAACATAG TGTATGAACG TCTAGCTGAC 
CACGGTCCTA AACTGGGTCC AGTTACCAGG AAAGAGCCTC TTGTCACCAG GTATTTCACC 
TTCCCCTTTG AGGAAATGAC TTTAGAGCAG GAGATGGAGT TTTTAAACCA ACCAGATAAG 
ATGTGCCACT TCTTGGCTCA CAACGGCTGG GTGATGGGTG ATGATCCATT GCGCAACTTT 
GCTGAACCAG GATCCAATGT GTACCTGCGT CGGGAGCTGA TCTGCTGGGG GGACAGTGTC 
AAACTCCGCT ATGGGAAAGG ACCTGAGGAT TGCCCCTACC TGTGGGCGCA CATGCAGAAA 
TACACTGAAA TTACTGCCAA ATATTTCCAC GGAGTCCGAT TGGACAACTG CCACTCTACG 
CCCTTGCATG TTGCTGAGTT CATGTTGGAT GTAGCCAGAA GCCAGTGTCC TAACCTGTAC 
GTGGTGGCTG AACTGTTCAC TGGCAGCGAG GAGCTGGATA ACATCTTTGT TACCAGACTA 
GGGATTACAT CCCTCATACG AGAGGCCATG TCCGCCAGAG ACAGCCATGA AGAGGGCCGG 
CTGGTCTACC GTTACGGTGG AGAGCCGGTC GGAGCCTTTG TTCAGCCCAG CCTGCGTCCT 
CTCATGCCTT CTATTGCTCA CGCCATGTTC TTGGATGTGA CCCATGACAA CGAGTGTCCC 
ATTGAGCTGC GGTCAGCTCT CGACTCGTTG CCCAGTTCTG CCATCGTCTC CATGGCCTGC 
TGTGCTACTG GCTCCACCAG AGGATATGAT GAGCTGGTCC CACATCAGAT ATCTGTGGTG 
AAGGAGGAGC GTTTTTATCC CAAGTGGAAT CCCTCTTCAG CTCCCACCTC TACTGGTGAA 
GTTGGCTTTA AGAGCGGCAT CATCACTGGG AAGCTGGCTC TCAACAAGCT GCACCAGGAG 
CTGGCTGCCC AGGGCTTTTC ACAGGTGTAT GTGGACCAGG TCGATGCAGA TATTGTTGCA 
GTTACTCGTC ACTGCCCCAG CACACACCAG TCTGTGGTAA CAGTGAGCAG GACAGCCTTC 
AAGAACCCCA AAACTCATCA GTACAGCAAC AATGTCCCAC CTATGTTCAT ACCTGGCAAG 
ATTGAAGAAA TTGTACTGGA GGCAAGGACA GTGGAGAGAG AGGCTAGGAG TTACAAGAAG 
GATGAGAAAT ACATAAACGG CATGCCAGAA TACACAGTGG AAATAAAAGA GCATATACCG 
ATGGAGGAGA GTGCAGTGGT GAAACATGCT GGAGTGACAT CTAAAGGCCG CTCAGAGTTT 
GTTCAAGAAA TTCAGTTCCA GAAATTGACC CCTGGTAGCA TCATTGCCTT CAGAGTCAGT 
TTGGATCCCA AGGCACAGAA GCTGGTGGGC GTGTTGAGGT TTTATCTGTC CCAGTTCAGT 
CCAAAGTACA GGAGGGGCAG TGTACCTGAT GAGAACCCAC CAGAAGTGCT GCAGAAACCC 
CTTACGCAGT TAATGTCTAA ACTGACGCTA GCCGACATGA ACATCTTGCT GTTTCGCTGC 
GAAGCAGAAG AACAAGAGGA CGGTGGAGGC TGTTACAGTA TCCCAGGCTG GGAGTGCCTG 
AAGTATGCAG GACTACAAGG TCTGATGTCT GTATTGGCGG ATATCCGTCC CACTAATGAC 
CTTGGCCATC CCCTGTGTGC TAACCTCAGA GAAGGAGACT GGCTCATAGA CTTTGTCTCC 
AATAGGCTGA AGCACAGAGA TGGACCTCTG GCACAGGTGG GTCAGTGGTT GGGTGCCATT 
TTTGACTACC TGAGGCACTT TCCTCGATAT CTCATCCCCT GTTACTTTGA CGCCATCTTG 
GTGTCAACCT ACACAACAGC TCTGGATGCC ACGTTCAAAC TCATGTCGAG CTTCATTGAG 
CGAGGTTCCA CGTTTGTCCG TCACCTGGCT CTCGGCTCGG TTCAGATGTG TGGAGTTGGA 
CGTTTTCCGG CTCTGCCTCC CATTTCAACA AAAGTGCAGG ATGTCCCATG CCGCATCAGT 
CCTATCACTG GGGAAAAGGA GCAGTGTTGT GTCTCACTGG CAGCAGGTCT TCCTCATTTT 
TCTGCTGGGA TTTTCCGTTG TTGGGGTAGA GATACTTTCA TCGCTCTCAG AGGACTTATG 
CTTATAACAG GGAGACACAC TGAGGCACGC AACATCATCC TGGCTTTTGC TGGAACCCTG 
CGTCACGGTT TAATCCCCAA CTTGCTGGGA GAAGGTCACA ATGCCAGATA CAACTGCAGG 
GATGCTGTGT GGTGGTGGCT GCAGTGCATT CAGGACTATA CCAATCTCGT TCCTGAGGGA 
CATGAAATTC TGCATTGCCC TGTCACCCGC ATGTACCCCA CAGACGACTG TGAGCCTTGC 
AAACCAGGGG AGGTTGAACA GCCATTGTAT GATGTCATCC AGGAGGCACT GCAGCGCCAT 
CTGGAGGGTA TTTCCTTCAG AGAGAGGAAT GCTGGACCCA AGATAGACAG GAACATGAGA 
GATGAAGGGT TCAATGTGGT TGCTAAAGTG GACCCAGCAA CAGGTTTTGT TACCGGAGGA 
AACCGCTTCA ACTGTGGCAC TTGGATGGAT AAAATGGGGG AGAGTGACAG AGCGAGGAAC 
AAGGGGATGC CTGCAACTCC AAGAGACGGC GCGGCGGTGG AGATCGTTGG TCTGAGTAAA 
TCAGCAGTAC GTTGGCTTGT GGAGCTGCAG TCTAAAGAAC TGTTCCCCTA TGATGGAGTT 
AAGGTGCTTA GAGATGGAAC AGATGTGTTC ATCTTGTACA CTGAGTGGAA CAAGCAGCTT 
CAGCAGTCCT TTGAAGTTGA ATTCTGGGTG TCTGGGGACT CACAGGACCC CAATGAGAAG 
CACCCTGATT TGGTGCATAA AAAGGGAATT TATAAAGATA GTTATGGAGC TTCCAATCCC 
TGGTGTGACT ACCAGCTGAG GCCCAACTTC ACCATCGCAA TGGTGGTGGC TCCAGAGCTG 
TTCACGTTAG AAAGAGCATG GAAGGCTTTG GAAATAGCTC AGAAGAAACT CTTGGGACCA 
CTGGGAATGA AAACTCTTGA CCCAGATGAC ATGGTCTACT GTGGTGTTTA CAATAATGCA 
CTGGACAGCG ACAACTACAA CCTGGCCAAG GGCTTTAACT ACCACCAAGG ACCTGAGTGG 
CTGTGGCCTG TTGGCTATTT TCTCCGTGCT AAGCTCTACT TTGCCAAGAA GTTAGGGGAG 
GAGGCCTACT CCAAGACCGT CATTCTAGTG AAAAATGTTC TGTCACGACA TTATACCCAC 
TTGGAGAGGT CTCTATGGAA AGGCTTACCA GAGCTGACCA ATGAGAATGG CCAGCATTGT 
CCCTTCAGCT GTGAGACCCA GGCCTGGTCT CTGGCTACTG TGCTGGAGGT GCTCCATGAT 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023190610.1
CDS	73..4218
Translation	MRYNMIHFTPLQTLGESRSCYSLADQLTFSLEFSPDGKSCTWEDVGRLVEKLRMEWNMVSITDVVYNHTAANSVWIKEHPECGYNLMNSPHLRPAWVLDRAIWHLTTRVADGRYKEKGLPSDITNECHLNALRRVFWEDMFPQIKLWEFYQVNVENAVEQFRILLQNGTKPDHSKTEGNKGLEIIQDLQCCRYGNRVNMDSALETFVPHSSSPQHIEECCGWLKQKLTEMNEEQYHIIHQHQEQAVNCIVENIVYERLADHGPKLGPVTRKEPLVTRYFTFPFEEMTLEQEMEFLNQPDKMCHFLAHNGWVMGDDPLRNFAEPGSNVYLRRELICWGDSVKLRYGKGPEDCPYLWAHMQKYTEITAKYFHGVRLDNCHSTPLHVAEFMLDVARSQCPNLYVVAELFTGSEELDNIFVTRLGITSLIREAMSARDSHEEGRLVYRYGGEPVGAFVQPSLRPLMPSIAHAMFLDVTHDNECPIELRSALDSLPSSAIVSMACCATGSTRGYDELVPHQISVVKEERFYPKWNPSSAPTSTGEVGFKSGIITGKLALNKLHQELAAQGFSQVYVDQVDADIVAVTRHCPSTHQSVVTVSRTAFKNPKTHQYSNNVPPMFIPGKIEEIVLEARTVEREARSYKKDEKYINGMPEYTVEIKEHIPMEESAVVKHAGVTSKGRSEFVQEIQFQKLTPGSIIAFRVSLDPKAQKLVGVLRFYLSQFSPKYRRGSVPDENPPEVLQKPLTQLMSKLTLADMNILLFRCEAEEQEDGGGCYSIPGWECLKYAGLQGLMSVLADIRPTNDLGHPLCANLREGDWLIDFVSNRLKHRDGPLAQVGQWLGAIFDYLRHFPRYLIPCYFDAILVSTYTTALDATFKLMSSFIERGSTFVRHLALGSVQMCGVGRFPALPPISTKVQDVPCRISPITGEKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLMLITGRHTEARNIILAFAGTLRHGLIPNLLGEGHNARYNCRDAVWWWLQCIQDYTNLVPEGHEILHCPVTRMYPTDDCEPCKPGEVEQPLYDVIQEALQRHLEGISFRERNAGPKIDRNMRDEGFNVVAKVDPATGFVTGGNRFNCGTWMDKMGESDRARNKGMPATPRDGAAVEIVGLSKSAVRWLVELQSKELFPYDGVKVLRDGTDVFILYTEWNKQLQQSFEVEFWVSGDSQDPNEKHPDLVHKKGIYKDSYGASNPWCDYQLRPNFTIAMVVAPELFTLERAWKALEIAQKKLLGPLGMKTLDPDDMVYCGVYNNALDSDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFAKKLGEEAYSKTVILVKNVLSRHYTHLERSLWKGLPELTNENGQHCPFSCETQAWSLATVLEVLHDL

Target ORF information:

RefSeq Version	XM_023334842.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023334842.1

ORF Insert Sequence:

ATGCGTTACA ACATGATCCA CTTCACTCCT CTGCAGACTT TGGGAGAGTC TAGGTCCTGT 
TACTCCTTAG CTGACCAGCT GACCTTTAGC CTGGAGTTCA GCCCAGATGG CAAGAGTTGC 
ACCTGGGAAG ATGTGGGAAG ACTGGTGGAA AAACTGAGGA TGGAATGGAA TATGGTATCA 
ATCACTGATG TGGTGTATAA TCACACTGCT GCAAACAGTG TGTGGATAAA GGAACATCCA 
GAGTGTGGCT ACAACCTGAT GAACTCTCCT CATCTGCGTC CTGCCTGGGT CTTGGACAGA 
GCTATCTGGC ATTTAACCAC TAGGGTAGCA GATGGACGCT ACAAAGAGAA GGGACTTCCT 
TCTGACATTA CCAACGAGTG CCACCTAAAT GCTCTCCGCA GGGTGTTTTG GGAGGACATG 
TTTCCTCAAA TCAAACTTTG GGAGTTCTAC CAGGTCAACG TGGAAAATGC TGTGGAGCAG 
TTCAGAATCC TGTTGCAAAA TGGCACAAAG CCAGATCACA GCAAGACTGA AGGAAATAAA 
GGATTAGAGA TCATTCAAGA CCTCCAGTGC TGTCGCTATG GCAACAGAGT AAACATGGAC 
TCAGCTTTGG AGACATTTGT ACCTCACAGC TCCTCTCCAC AGCACATTGA AGAGTGCTGT 
GGTTGGCTGA AACAGAAGTT GACTGAGATG AATGAAGAAC AGTACCACAT CATACACCAG 
CATCAGGAAC AGGCAGTCAA CTGCATTGTG GAGAACATAG TGTATGAACG TCTAGCTGAC 
CACGGTCCTA AACTGGGTCC AGTTACCAGG AAAGAGCCTC TTGTCACCAG GTATTTCACC 
TTCCCCTTTG AGGAAATGAC TTTAGAGCAG GAGATGGAGT TTTTAAACCA ACCAGATAAG 
ATGTGCCACT TCTTGGCTCA CAACGGCTGG GTGATGGGTG ATGATCCATT GCGCAACTTT 
GCTGAACCAG GATCCAATGT GTACCTGCGT CGGGAGCTGA TCTGCTGGGG GGACAGTGTC 
AAACTCCGCT ATGGGAAAGG ACCTGAGGAT TGCCCCTACC TGTGGGCGCA CATGCAGAAA 
TACACTGAAA TTACTGCCAA ATATTTCCAC GGAGTCCGAT TGGACAACTG CCACTCTACG 
CCCTTGCATG TTGCTGAGTT CATGTTGGAT GTAGCCAGAA GCCAGTGTCC TAACCTGTAC 
GTGGTGGCTG AACTGTTCAC TGGCAGCGAG GAGCTGGATA ACATCTTTGT TACCAGACTA 
GGGATTACAT CCCTCATACG AGAGGCCATG TCCGCCAGAG ACAGCCATGA AGAGGGCCGG 
CTGGTCTACC GTTACGGTGG AGAGCCGGTC GGAGCCTTTG TTCAGCCCAG CCTGCGTCCT 
CTCATGCCTT CTATTGCTCA CGCCATGTTC TTGGATGTGA CCCATGACAA CGAGTGTCCC 
ATTGAGCTGC GGTCAGCTCT CGACTCGTTG CCCAGTTCTG CCATCGTCTC CATGGCCTGC 
TGTGCTACTG GCTCCACCAG AGGATATGAT GAGCTGGTCC CACATCAGAT ATCTGTGGTG 
AAGGAGGAGC GTTTTTATCC CAAGTGGAAT CCCTCTTCAG CTCCCACCTC TACTGGTGAA 
GTTGGCTTTA AGAGCGGCAT CATCACTGGG AAGCTGGCTC TCAACAAGCT GCACCAGGAG 
CTGGCTGCCC AGGGCTTTTC ACAGGTGTAT GTGGACCAGG TCGATGCAGA TATTGTTGCA 
GTTACTCGTC ACTGCCCCAG CACACACCAG TCTGTGGTAA CAGTGAGCAG GACAGCCTTC 
AAGAACCCCA AAACTCATCA GTACAGCAAC AATGTCCCAC CTATGTTCAT ACCTGGCAAG 
ATTGAAGAAA TTGTACTGGA GGCAAGGACA GTGGAGAGAG AGGCTAGGAG TTACAAGAAG 
GATGAGAAAT ACATAAACGG CATGCCAGAA TACACAGTGG AAATAAAAGA GCATATACCG 
ATGGAGGAGA GTGCAGTGGT GAAACATGCT GGAGTGACAT CTAAAGGCCG CTCAGAGTTT 
GTTCAAGAAA TTCAGTTCCA GAAATTGACC CCTGGTAGCA TCATTGCCTT CAGAGTCAGT 
TTGGATCCCA AGGCACAGAA GCTGGTGGGC GTGTTGAGGT TTTATCTGTC CCAGTTCAGT 
CCAAAGTACA GGAGGGGCAG TGTACCTGAT GAGAACCCAC CAGAAGTGCT GCAGAAACCC 
CTTACGCAGT TAATGTCTAA ACTGACGCTA GCCGACATGA ACATCTTGCT GTTTCGCTGC 
GAAGCAGAAG AACAAGAGGA CGGTGGAGGC TGTTACAGTA TCCCAGGCTG GGAGTGCCTG 
AAGTATGCAG GACTACAAGG TCTGATGTCT GTATTGGCGG ATATCCGTCC CACTAATGAC 
CTTGGCCATC CCCTGTGTGC TAACCTCAGA GAAGGAGACT GGCTCATAGA CTTTGTCTCC 
AATAGGCTGA AGCACAGAGA TGGACCTCTG GCACAGGTGG GTCAGTGGTT GGGTGCCATT 
TTTGACTACC TGAGGCACTT TCCTCGATAT CTCATCCCCT GTTACTTTGA CGCCATCTTG 
GTGTCAACCT ACACAACAGC TCTGGATGCC ACGTTCAAAC TCATGTCGAG CTTCATTGAG 
CGAGGTTCCA CGTTTGTCCG TCACCTGGCT CTCGGCTCGG TTCAGATGTG TGGAGTTGGA 
CGTTTTCCGG CTCTGCCTCC CATTTCAACA AAAGTGCAGG ATGTCCCATG CCGCATCAGT 
CCTATCACTG GGGAAAAGGA GCAGTGTTGT GTCTCACTGG CAGCAGGTCT TCCTCATTTT 
TCTGCTGGGA TTTTCCGTTG TTGGGGTAGA GATACTTTCA TCGCTCTCAG AGGACTTATG 
CTTATAACAG GGAGACACAC TGAGGCACGC AACATCATCC TGGCTTTTGC TGGAACCCTG 
CGTCACGGTT TAATCCCCAA CTTGCTGGGA GAAGGTCACA ATGCCAGATA CAACTGCAGG 
GATGCTGTGT GGTGGTGGCT GCAGTGCATT CAGGACTATA CCAATCTCGT TCCTGAGGGA 
CATGAAATTC TGCATTGCCC TGTCACCCGC ATGTACCCCA CAGACGACTG TGAGCCTTGC 
AAACCAGGGG AGGTTGAACA GCCATTGTAT GATGTCATCC AGGAGGCACT GCAGCGCCAT 
CTGGAGGGTA TTTCCTTCAG AGAGAGGAAT GCTGGACCCA AGATAGACAG GAACATGAGA 
GATGAAGGGT TCAATGTGGT TGCTAAAGTG GACCCAGCAA CAGGTTTTGT TACCGGAGGA 
AACCGCTTCA ACTGTGGCAC TTGGATGGAT AAAATGGGGG AGAGTGACAG AGCGAGGAAC 
AAGGGGATGC CTGCAACTCC AAGAGACGGC GCGGCGGTGG AGATCGTTGG TCTGAGTAAA 
TCAGCAGTAC GTTGGCTTGT GGAGCTGCAG TCTAAAGAAC TGTTCCCCTA TGATGGAGTT 
AAGGTGCTTA GAGATGGAAC AGATGTGTTC ATCTTGTACA CTGAGTGGAA CAAGCAGCTT 
CAGCAGTCCT TTGAAGTTGA ATTCTGGGTG TCTGGGGACT CACAGGACCC CAATGAGAAG 
CACCCTGATT TGGTGCATAA AAAGGGAATT TATAAAGATA GTTATGGAGC TTCCAATCCC 
TGGTGTGACT ACCAGCTGAG GCCCAACTTC ACCATCGCAA TGGTGGTGGC TCCAGAGCTG 
TTCACGTTAG AAAGAGCATG GAAGGCTTTG GAAATAGCTC AGAAGAAACT CTTGGGACCA 
CTGGGAATGA AAACTCTTGA CCCAGATGAC ATGGTCTACT GTGGTGTTTA CAATAATGCA 
CTGGACAGCG ACAACTACAA CCTGGCCAAG GGCTTTAACT ACCACCAAGG ACCTGAGTGG 
CTGTGGCCTG TTGGCTATTT TCTCCGTGCT AAGCTCTACT TTGCCAAGAA GTTAGGGGAG 
GAGGCCTACT CCAAGACCGT CATTCTAGTG AAAAATGTTC TGTCACGACA TTATACCCAC 
TTGGAGAGGT CTCTATGGAA AGGCTTACCA GAGCTGACCA ATGAGAATGG CCAGCATTGT 
CCCTTCAGCT GTGAGACCCA GGCCTGGTCT CTGGCTACTG TGCTGGAGGT GCTCCATGAT 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl ( XM_014472572.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_014328058.1 Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), mRNA.

Price & Availability↑

CloneID	OXi14483
Clone ID Related Accession (Same CDS sequence)	XM_014472572.1
Accession Version	XM_014472572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1445702400000
Organism	Xiphophorus maculatus(Southern platyfish)
Product	glycogen debranching enzyme
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005372430.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xiphophorus maculatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 15% of CDS bases ##RefSeq-Attributes-END##

ATGATGCTGC CTCAGCACCT CAAGCAAATC AGAGTCCTCA TGCTTAACGG AAAGGAGAAT 
TTAGAGAGAA CTTTGTTCAG ACTTGAACAA GGTTTTGAGC TCCAGTTCCG CCTGGGTCCA 
AGCCTTCAAG GAAGGAGAGT CATAGTTCAC ACCAACTACC CACTTGATGG ACAGAAGTTC 
ATTCGAAACA ACTTCCGTGT TTTAGCCTGG AACTACCCAA CAGGAAGGGA GGATGACTCT 
GATAAGTACT GCTCCCTAGA GCTCAAGATT GCTGGATCCT ACCAGTACTA CTTTGGATAT 
GCCAAGGAGA ACGAGCTAAC GACTGCTAAG GCTATCCAGA ATCTATGCAA CAATATCAAA 
GAAATGCAAA ATGACATCAT CGCTATGAAA AACGACATCA AACAAGACCC GAACAGCTTT 
AAGAGTGAAA TGAACAAGAA ATTGGAAGAG GTCAGCTCGG ACATTCGAAA TCAAGCTGCT 
AGGCTAACTG AAGCGGAACA ATGGGTGAAC GAGCTGGAGA TGGTGAATAT GGACTTGAGA 
GATTCTTTGT CTTACTGCCT CAAACAGCAG AAAACTCTCC TGGACAAAGT TACTGATTTA 
GAAGGAAGAT CCCTACGCAA TAATATCCGC ATTTACGGCT TTAAAGAAGA AGCTGAAGGC 
AACGCCATGC AGACGTTCAT TGAAAACCTT CTGAAAGAGC AGCTCTCCAT CAACCAAGAT 
TTGCAGATTC AACGAGCACA TCGTTCACTG GGACCAAGAC CTCGAGACGC ATCAATTTCA 
AGATCCATAC TCGTCAACTT TCACAGATAC GACATAAAAG AGAAAATCCT TAAAGCAGCG 
TGGGGAAAGA AGATATTTTA TGATGGCAAA CTGACCTCAT TTGCGCACGA TCTACCGACC 
GAGATAAATA ATAAGCTTAA GGAATACAAG AACATCAAGA AGAGTCTCAA AGAGGGGAAA 
ATACGGTTCC AAACACCTTA CCCGGCTAAG ATGAGGATTC ACTGGGACGA CGGACCACAC 
CTGTACGCGA GTGCTTCAGA AGCAGTGGAC GACATGAGGA AGATTGGATA CCGGGCTTAC 
TCCACACCTG GTTACAACAT GATCCACTTC ACTCCTCTGC AGACTTTGGG AGAGTCTAGG 
TCCTGTTACT CCTTAGCTGA CCAGCTGACC TTTAGCCTGG AGTTCAGCCC AGATGGCAAG 
AGTTGCACCT GGGAAGATGT GGGAAGACTG GTGGAAAAAC TGAGGATGGA ATGGAATATG 
GTATCAATCA CTGATGTGGT GTATAATCAC ACTGCTGCAA ACAGTGTGTG GATAAAGGAA 
CATCCAGAGT GTGGCTACAA CCTGATGAAC TCTCCTCATC TGCGTCCTGC CTGGGTCTTG 
GACAGAGCTA TCTGGCATTT AACCACTAGG GTAGCAGATG GACGCTACAA AGAGAAGGGA 
CTTCCTTCTG ACATTACCAA CGAGTGCCAC CTAAATGCTC TCCGCAGGGT GTTTTGGGAG 
GACATGTTTC CTCAAATCAA ACTTTGGGAG TTCTACCAGG TCAACGTGGA AAATGCTGTG 
GAGCAGTTCA GAATCCTGTT GCAAAATGGC ACAAAGCCAG ATCACAGCAA GACTGAAGGA 
AATAAAGGAT TAGAGATCAT TCAAGACCTC CAGTGCTGTC GCTATGGCAA CAGAGTAAAC 
ATGGACTCAG CTTTGGAGAC ATTTGTACCT CACAGCTCCT CTCCACAGCA CATTGAAGAG 
TGCTGTGGTT GGCTGAAACA GAAGTTGACT GAGATGAATG AAGAACAGTA CCACATCATA 
CACCAGCATC AGGAACAGGC AGTCAACTGC ATTGTGGAGA ACATAGTGTA TGAACGTCTA 
GCTGACCACG GTCCTAAACT GGGTCCAGTT ACCAGGAAAG AGCCTCTTGT CACCAGGTAT 
TTCACCTTCC CCTTTGAGGA AATGACTTTA GAGCAGGAGA TGGAGTTTTT AAACCAACCA 
GATAAGATGT GCCACTTCTT GGCTCACAAC GGCTGGGTGA TGGGTGATGA TCCATTGCGC 
AACTTTGCTG AACCAGGATC CAATGTGTAC CTGCGTCGGG AGCTGATCTG CTGGGGGGAC 
AGTGTCAAAC TCCGCTATGG GAAAGGACCT GAGGATTGCC CCTACCTGTG GGCGCACATG 
CAGAAATACA CTGAAATTAC TGCCAAATAT TTCCACGGAG TCCGATTGGA CAACTGCCAC 
TCTACGCCCT TGCATGTTGC TGAGTTCATG TTGGATGTAG CCAGAAGCCA GTGTCCTAAC 
CTGTACGTGG TGGCTGAACT GTTCACTGGC AGCGAGGAGC TGGATAACAT CTTTGTTACC 
AGACTAGGGA TTACATCCCT CATACGAGAG GCCATGTCCG CCAGAGACAG CCATGAAGAG 
GGCCGGCTGG TCTACCGTTA CGGTGGAGAG CCGGTCGGAG CCTTTGTTCA GCCCAGCCTG 
CGTCCTCTCA TGCCTTCTAT TGCTCACGCC ATGTTCTTGG ATGTGACCCA TGACAACGAG 
TGTCCCATTG AGCTGCGGTC AGCTCTCGAC TCGTTGCCCA GTTCTGCCAT CGTCTCCATG 
GCCTGCTGTG CTACTGGCTC CACCAGAGGA TATGATGAGC TGGTCCCACA TCAGATATCT 
GTGGTGAAGG AGGAGCGTTT TTATCCCAAG TGGAATCCCT CTTCAGCTCC CACCTCTACT 
GGTGAAGTTG GCTTTAAGAG CGGCATCATC ACTGGGAAGC TGGCTCTCAA CAAGCTGCAC 
CAGGAGCTGG CTGCCCAGGG CTTTTCACAG GTGTATGTGG ACCAGGTCGA TGCAGATATT 
GTTGCAGTTA CTCGTCACTG CCCCAGCACA CACCAGTCTG TGGTAACAGT GAGCAGGACA 
GCCTTCAAGA ACCCCAAAAC TCATCAGTAC AGCAACAATG TCCCACCTAT GTTCATACCT 
GGCAAGATTG AAGAAATTGT ACTGGAGGCA AGGACAGTGG AGAGAGAGGC TAGGAGTTAC 
AAGAAGGATG AGAAATACAT AAACGGCATG CCAGAATACA CAGTGGAAAT AAAAGAGCAT 
ATACCGATGG AGGAGAGTGC AGTGGTGAAA CATGCTGGAG TGACATCTAA AGGCCGCTCA 
GAGTTTGTTC AAGAAATTCA GTTCCAGAAA TTGACCCCTG GTAGCATCAT TGCCTTCAGA 
GTCAGTTTGG ATCCCAAGGC ACAGAAGCTG GTGGGCGTGT TGAGGTTTTA TCTGTCCCAG 
TTCAGTCCAA AGTACAGGAG GGGCAGTGTA CCTGATGAGA ACCCACCAGA AGTGCTGCAG 
AAACCCCTTA CGCAGTTAAT GTCTAAACTG ACGCTAGCCG ACATGAACAT CTTGCTGTTT 
CGCTGCGAAG CAGAAGAACA AGAGGACGGT GGAGGCTGTT ACAGTATCCC AGGCTGGGAG 
TGCCTGAAGT ATGCAGGACT ACAAGGTCTG ATGTCTGTAT TGGCGGATAT CCGTCCCACT 
AATGACCTTG GCCATCCCCT GTGTGCTAAC CTCAGAGAAG GAGACTGGCT CATAGACTTT 
GTCTCCAATA GGCTGAAGCA CAGAGATGGA CCTCTGGCAC AGGTGGGTCA GTGGTTGGGT 
GCCATTTTTG ACTACCTGAG GCACTTTCCT CGATATCTCA TCCCCTGTTA CTTTGACGCC 
ATCTTGGTGT CAACCTACAC AACAGCTCTG GATGCCACGT TCAAACTCAT GTCGAGCTTC 
ATTGAGCGAG GTTCCACGTT TGTCCGTCAC CTGGCTCTCG GCTCGGTTCA GATGTGTGGA 
GTTGGACGTT TTCCGGCTCT GCCTCCCATT TCAACAAAAG TGCAGGATGT CCCATGCCGC 
ATCAGTCCTA TCACTGGGGA AAAGGAGCAG TGTTGTGTCT CACTGGCAGC AGGTCTTCCT 
CATTTTTCTG CTGGGATTTT CCGTTGTTGG GGTAGAGATA CTTTCATCGC TCTCAGAGGA 
CTTATGCTTA TAACAGGGAG ACACACTGAG GCACGCAACA TCATCCTGGC TTTTGCTGGA 
ACCCTGCGTC ACGGTTTAAT CCCCAACTTG CTGGGAGAAG GTCACAATGC CAGATACAAC 
TGCAGGGATG CTGTGTGGTG GTGGCTGCAG TGCATTCAGG ACTATACCAA TCTCGTTCCT 
GAGGGACATG AAATTCTGCA TTGCCCTGTC ACCCGCATGT ACCCCACAGA CGACTGTGAG 
CCTTGCAAAC CAGGGGAGGT TGAACAGCCA TTGTATGATG TCATCCAGGA GGCACTGCAG 
CGCCATCTGG AGGGTATTTC CTTCAGAGAG AGGAATGCTG GACCCAAGAT AGACAGGAAC 
ATGAGAGATG AAGGGTTCAA TGTGGTTGCT AAAGTGGACC CAGCAACAGG TTTTGTTACC 
GGAGGAAACC GCTTCAACTG TGGCACTTGG ATGGATAAAA TGGGGGAGAG TGACAGAGCG 
AGGAACAAGG GGATGCCTGC AACTCCAAGA GACGGCGCGG CGGTGGAGAT CGTTGGTCTG 
AGTAAATCAG CAGTACGTTG GCTTGTGGAG CTGCAGTCTA AAGAACTGTT CCCCTATGAT 
GGAGTTAAGG TGCTTAGAGA TGGAACAGAT GTGTTCATCT TGTACACTGA GTGGAACAAG 
CAGCTTCAGC AGTCCTTTGA AGTTGAATTC TGGGTGTCTG GGGACTCACA GGACCCCAAT 
GAGAAGCACC CTGATTTGGT GCATAAAAAG GGAATTTATA AAGATAGTTA TGGAGCTTCC 
AATCCCTGGT GTGACTACCA GCTGAGGCCC AACTTCACCA TCGCAATGGT GGTGGCTCCA 
GAGCTGTTCA CGTTAGAAAG AGCATGGAAG GCTTTGGAAA TAGCTCAGAA GAAACTCTTG 
GGACCACTGG GAATGAAAAC TCTTGACCCA GATGACATGG TCTACTGTGG TGTTTACAAT 
AATGCACTGG ACAGCGACAA CTACAACCTG GCCAAGGGCT TTAACTACCA CCAAGGACCT 
GAGTGGCTGT GGCCTGTTGG CTATTTTCTC CGTGCTAAGC TCTACTTTGC CAAGAAGTTA 
GGGGAGGAGG CCTACTCCAA GACCGTCATT CTAGTGAAAA ATGTTCTGTC ACGACATTAT 
ACCCACTTGG AGAGGTCTCT ATGGAAAGGC TTACCAGAGC TGACCAATGA GAATGGCCAG 
CATTGTCCCT TCAGCTGTGA GACCCAGGCC TGGTCTCTGG CTACTGTGCT GGAGGTGCTC 
CATGATCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014328058.1
CDS	123..5354
Translation	MMLPQHLKQIRVLMLNGKENLERTLFRLEQGFELQFRLGPSLQGRRVIVHTNYPLDGQKFIRNNFRVLAWNYPTGREDDSDKYCSLELKIAGSYQYYFGYAKENELTTAKAIQNLCNNIKEMQNDIIAMKNDIKQDPNSFKSEMNKKLEEVSSDIRNQAARLTEAEQWVNELEMVNMDLRDSLSYCLKQQKTLLDKVTDLEGRSLRNNIRIYGFKEEAEGNAMQTFIENLLKEQLSINQDLQIQRAHRSLGPRPRDASISRSILVNFHRYDIKEKILKAAWGKKIFYDGKLTSFAHDLPTEINNKLKEYKNIKKSLKEGKIRFQTPYPAKMRIHWDDGPHLYASASEAVDDMRKIGYRAYSTPGYNMIHFTPLQTLGESRSCYSLADQLTFSLEFSPDGKSCTWEDVGRLVEKLRMEWNMVSITDVVYNHTAANSVWIKEHPECGYNLMNSPHLRPAWVLDRAIWHLTTRVADGRYKEKGLPSDITNECHLNALRRVFWEDMFPQIKLWEFYQVNVENAVEQFRILLQNGTKPDHSKTEGNKGLEIIQDLQCCRYGNRVNMDSALETFVPHSSSPQHIEECCGWLKQKLTEMNEEQYHIIHQHQEQAVNCIVENIVYERLADHGPKLGPVTRKEPLVTRYFTFPFEEMTLEQEMEFLNQPDKMCHFLAHNGWVMGDDPLRNFAEPGSNVYLRRELICWGDSVKLRYGKGPEDCPYLWAHMQKYTEITAKYFHGVRLDNCHSTPLHVAEFMLDVARSQCPNLYVVAELFTGSEELDNIFVTRLGITSLIREAMSARDSHEEGRLVYRYGGEPVGAFVQPSLRPLMPSIAHAMFLDVTHDNECPIELRSALDSLPSSAIVSMACCATGSTRGYDELVPHQISVVKEERFYPKWNPSSAPTSTGEVGFKSGIITGKLALNKLHQELAAQGFSQVYVDQVDADIVAVTRHCPSTHQSVVTVSRTAFKNPKTHQYSNNVPPMFIPGKIEEIVLEARTVEREARSYKKDEKYINGMPEYTVEIKEHIPMEESAVVKHAGVTSKGRSEFVQEIQFQKLTPGSIIAFRVSLDPKAQKLVGVLRFYLSQFSPKYRRGSVPDENPPEVLQKPLTQLMSKLTLADMNILLFRCEAEEQEDGGGCYSIPGWECLKYAGLQGLMSVLADIRPTNDLGHPLCANLREGDWLIDFVSNRLKHRDGPLAQVGQWLGAIFDYLRHFPRYLIPCYFDAILVSTYTTALDATFKLMSSFIERGSTFVRHLALGSVQMCGVGRFPALPPISTKVQDVPCRISPITGEKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLMLITGRHTEARNIILAFAGTLRHGLIPNLLGEGHNARYNCRDAVWWWLQCIQDYTNLVPEGHEILHCPVTRMYPTDDCEPCKPGEVEQPLYDVIQEALQRHLEGISFRERNAGPKIDRNMRDEGFNVVAKVDPATGFVTGGNRFNCGTWMDKMGESDRARNKGMPATPRDGAAVEIVGLSKSAVRWLVELQSKELFPYDGVKVLRDGTDVFILYTEWNKQLQQSFEVEFWVSGDSQDPNEKHPDLVHKKGIYKDSYGASNPWCDYQLRPNFTIAMVVAPELFTLERAWKALEIAQKKLLGPLGMKTLDPDDMVYCGVYNNALDSDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFAKKLGEEAYSKTVILVKNVLSRHYTHLERSLWKGLPELTNENGQHCPFSCETQAWSLATVLEVLHDL

Target ORF information:

RefSeq Version	XM_014472572.1
Organism	Xiphophorus maculatus(Southern platyfish)
Definition	Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014472572.1

ORF Insert Sequence:

ATGATGCTGC CTCAGCACCT CAAGCAAATC AGAGTCCTCA TGCTTAACGG AAAGGAGAAT 
TTAGAGAGAA CTTTGTTCAG ACTTGAACAA GGTTTTGAGC TCCAGTTCCG CCTGGGTCCA 
AGCCTTCAAG GAAGGAGAGT CATAGTTCAC ACCAACTACC CACTTGATGG ACAGAAGTTC 
ATTCGAAACA ACTTCCGTGT TTTAGCCTGG AACTACCCAA CAGGAAGGGA GGATGACTCT 
GATAAGTACT GCTCCCTAGA GCTCAAGATT GCTGGATCCT ACCAGTACTA CTTTGGATAT 
GCCAAGGAGA ACGAGCTAAC GACTGCTAAG GCTATCCAGA ATCTATGCAA CAATATCAAA 
GAAATGCAAA ATGACATCAT CGCTATGAAA AACGACATCA AACAAGACCC GAACAGCTTT 
AAGAGTGAAA TGAACAAGAA ATTGGAAGAG GTCAGCTCGG ACATTCGAAA TCAAGCTGCT 
AGGCTAACTG AAGCGGAACA ATGGGTGAAC GAGCTGGAGA TGGTGAATAT GGACTTGAGA 
GATTCTTTGT CTTACTGCCT CAAACAGCAG AAAACTCTCC TGGACAAAGT TACTGATTTA 
GAAGGAAGAT CCCTACGCAA TAATATCCGC ATTTACGGCT TTAAAGAAGA AGCTGAAGGC 
AACGCCATGC AGACGTTCAT TGAAAACCTT CTGAAAGAGC AGCTCTCCAT CAACCAAGAT 
TTGCAGATTC AACGAGCACA TCGTTCACTG GGACCAAGAC CTCGAGACGC ATCAATTTCA 
AGATCCATAC TCGTCAACTT TCACAGATAC GACATAAAAG AGAAAATCCT TAAAGCAGCG 
TGGGGAAAGA AGATATTTTA TGATGGCAAA CTGACCTCAT TTGCGCACGA TCTACCGACC 
GAGATAAATA ATAAGCTTAA GGAATACAAG AACATCAAGA AGAGTCTCAA AGAGGGGAAA 
ATACGGTTCC AAACACCTTA CCCGGCTAAG ATGAGGATTC ACTGGGACGA CGGACCACAC 
CTGTACGCGA GTGCTTCAGA AGCAGTGGAC GACATGAGGA AGATTGGATA CCGGGCTTAC 
TCCACACCTG GTTACAACAT GATCCACTTC ACTCCTCTGC AGACTTTGGG AGAGTCTAGG 
TCCTGTTACT CCTTAGCTGA CCAGCTGACC TTTAGCCTGG AGTTCAGCCC AGATGGCAAG 
AGTTGCACCT GGGAAGATGT GGGAAGACTG GTGGAAAAAC TGAGGATGGA ATGGAATATG 
GTATCAATCA CTGATGTGGT GTATAATCAC ACTGCTGCAA ACAGTGTGTG GATAAAGGAA 
CATCCAGAGT GTGGCTACAA CCTGATGAAC TCTCCTCATC TGCGTCCTGC CTGGGTCTTG 
GACAGAGCTA TCTGGCATTT AACCACTAGG GTAGCAGATG GACGCTACAA AGAGAAGGGA 
CTTCCTTCTG ACATTACCAA CGAGTGCCAC CTAAATGCTC TCCGCAGGGT GTTTTGGGAG 
GACATGTTTC CTCAAATCAA ACTTTGGGAG TTCTACCAGG TCAACGTGGA AAATGCTGTG 
GAGCAGTTCA GAATCCTGTT GCAAAATGGC ACAAAGCCAG ATCACAGCAA GACTGAAGGA 
AATAAAGGAT TAGAGATCAT TCAAGACCTC CAGTGCTGTC GCTATGGCAA CAGAGTAAAC 
ATGGACTCAG CTTTGGAGAC ATTTGTACCT CACAGCTCCT CTCCACAGCA CATTGAAGAG 
TGCTGTGGTT GGCTGAAACA GAAGTTGACT GAGATGAATG AAGAACAGTA CCACATCATA 
CACCAGCATC AGGAACAGGC AGTCAACTGC ATTGTGGAGA ACATAGTGTA TGAACGTCTA 
GCTGACCACG GTCCTAAACT GGGTCCAGTT ACCAGGAAAG AGCCTCTTGT CACCAGGTAT 
TTCACCTTCC CCTTTGAGGA AATGACTTTA GAGCAGGAGA TGGAGTTTTT AAACCAACCA 
GATAAGATGT GCCACTTCTT GGCTCACAAC GGCTGGGTGA TGGGTGATGA TCCATTGCGC 
AACTTTGCTG AACCAGGATC CAATGTGTAC CTGCGTCGGG AGCTGATCTG CTGGGGGGAC 
AGTGTCAAAC TCCGCTATGG GAAAGGACCT GAGGATTGCC CCTACCTGTG GGCGCACATG 
CAGAAATACA CTGAAATTAC TGCCAAATAT TTCCACGGAG TCCGATTGGA CAACTGCCAC 
TCTACGCCCT TGCATGTTGC TGAGTTCATG TTGGATGTAG CCAGAAGCCA GTGTCCTAAC 
CTGTACGTGG TGGCTGAACT GTTCACTGGC AGCGAGGAGC TGGATAACAT CTTTGTTACC 
AGACTAGGGA TTACATCCCT CATACGAGAG GCCATGTCCG CCAGAGACAG CCATGAAGAG 
GGCCGGCTGG TCTACCGTTA CGGTGGAGAG CCGGTCGGAG CCTTTGTTCA GCCCAGCCTG 
CGTCCTCTCA TGCCTTCTAT TGCTCACGCC ATGTTCTTGG ATGTGACCCA TGACAACGAG 
TGTCCCATTG AGCTGCGGTC AGCTCTCGAC TCGTTGCCCA GTTCTGCCAT CGTCTCCATG 
GCCTGCTGTG CTACTGGCTC CACCAGAGGA TATGATGAGC TGGTCCCACA TCAGATATCT 
GTGGTGAAGG AGGAGCGTTT TTATCCCAAG TGGAATCCCT CTTCAGCTCC CACCTCTACT 
GGTGAAGTTG GCTTTAAGAG CGGCATCATC ACTGGGAAGC TGGCTCTCAA CAAGCTGCAC 
CAGGAGCTGG CTGCCCAGGG CTTTTCACAG GTGTATGTGG ACCAGGTCGA TGCAGATATT 
GTTGCAGTTA CTCGTCACTG CCCCAGCACA CACCAGTCTG TGGTAACAGT GAGCAGGACA 
GCCTTCAAGA ACCCCAAAAC TCATCAGTAC AGCAACAATG TCCCACCTAT GTTCATACCT 
GGCAAGATTG AAGAAATTGT ACTGGAGGCA AGGACAGTGG AGAGAGAGGC TAGGAGTTAC 
AAGAAGGATG AGAAATACAT AAACGGCATG CCAGAATACA CAGTGGAAAT AAAAGAGCAT 
ATACCGATGG AGGAGAGTGC AGTGGTGAAA CATGCTGGAG TGACATCTAA AGGCCGCTCA 
GAGTTTGTTC AAGAAATTCA GTTCCAGAAA TTGACCCCTG GTAGCATCAT TGCCTTCAGA 
GTCAGTTTGG ATCCCAAGGC ACAGAAGCTG GTGGGCGTGT TGAGGTTTTA TCTGTCCCAG 
TTCAGTCCAA AGTACAGGAG GGGCAGTGTA CCTGATGAGA ACCCACCAGA AGTGCTGCAG 
AAACCCCTTA CGCAGTTAAT GTCTAAACTG ACGCTAGCCG ACATGAACAT CTTGCTGTTT 
CGCTGCGAAG CAGAAGAACA AGAGGACGGT GGAGGCTGTT ACAGTATCCC AGGCTGGGAG 
TGCCTGAAGT ATGCAGGACT ACAAGGTCTG ATGTCTGTAT TGGCGGATAT CCGTCCCACT 
AATGACCTTG GCCATCCCCT GTGTGCTAAC CTCAGAGAAG GAGACTGGCT CATAGACTTT 
GTCTCCAATA GGCTGAAGCA CAGAGATGGA CCTCTGGCAC AGGTGGGTCA GTGGTTGGGT 
GCCATTTTTG ACTACCTGAG GCACTTTCCT CGATATCTCA TCCCCTGTTA CTTTGACGCC 
ATCTTGGTGT CAACCTACAC AACAGCTCTG GATGCCACGT TCAAACTCAT GTCGAGCTTC 
ATTGAGCGAG GTTCCACGTT TGTCCGTCAC CTGGCTCTCG GCTCGGTTCA GATGTGTGGA 
GTTGGACGTT TTCCGGCTCT GCCTCCCATT TCAACAAAAG TGCAGGATGT CCCATGCCGC 
ATCAGTCCTA TCACTGGGGA AAAGGAGCAG TGTTGTGTCT CACTGGCAGC AGGTCTTCCT 
CATTTTTCTG CTGGGATTTT CCGTTGTTGG GGTAGAGATA CTTTCATCGC TCTCAGAGGA 
CTTATGCTTA TAACAGGGAG ACACACTGAG GCACGCAACA TCATCCTGGC TTTTGCTGGA 
ACCCTGCGTC ACGGTTTAAT CCCCAACTTG CTGGGAGAAG GTCACAATGC CAGATACAAC 
TGCAGGGATG CTGTGTGGTG GTGGCTGCAG TGCATTCAGG ACTATACCAA TCTCGTTCCT 
GAGGGACATG AAATTCTGCA TTGCCCTGTC ACCCGCATGT ACCCCACAGA CGACTGTGAG 
CCTTGCAAAC CAGGGGAGGT TGAACAGCCA TTGTATGATG TCATCCAGGA GGCACTGCAG 
CGCCATCTGG AGGGTATTTC CTTCAGAGAG AGGAATGCTG GACCCAAGAT AGACAGGAAC 
ATGAGAGATG AAGGGTTCAA TGTGGTTGCT AAAGTGGACC CAGCAACAGG TTTTGTTACC 
GGAGGAAACC GCTTCAACTG TGGCACTTGG ATGGATAAAA TGGGGGAGAG TGACAGAGCG 
AGGAACAAGG GGATGCCTGC AACTCCAAGA GACGGCGCGG CGGTGGAGAT CGTTGGTCTG 
AGTAAATCAG CAGTACGTTG GCTTGTGGAG CTGCAGTCTA AAGAACTGTT CCCCTATGAT 
GGAGTTAAGG TGCTTAGAGA TGGAACAGAT GTGTTCATCT TGTACACTGA GTGGAACAAG 
CAGCTTCAGC AGTCCTTTGA AGTTGAATTC TGGGTGTCTG GGGACTCACA GGACCCCAAT 
GAGAAGCACC CTGATTTGGT GCATAAAAAG GGAATTTATA AAGATAGTTA TGGAGCTTCC 
AATCCCTGGT GTGACTACCA GCTGAGGCCC AACTTCACCA TCGCAATGGT GGTGGCTCCA 
GAGCTGTTCA CGTTAGAAAG AGCATGGAAG GCTTTGGAAA TAGCTCAGAA GAAACTCTTG 
GGACCACTGG GAATGAAAAC TCTTGACCCA GATGACATGG TCTACTGTGG TGTTTACAAT 
AATGCACTGG ACAGCGACAA CTACAACCTG GCCAAGGGCT TTAACTACCA CCAAGGACCT 
GAGTGGCTGT GGCCTGTTGG CTATTTTCTC CGTGCTAAGC TCTACTTTGC CAAGAAGTTA 
GGGGAGGAGG CCTACTCCAA GACCGTCATT CTAGTGAAAA ATGTTCTGTC ACGACATTAT 
ACCCACTTGG AGAGGTCTCT ATGGAAAGGC TTACCAGAGC TGACCAATGA GAATGGCCAG 
CATTGTCCCT TCAGCTGTGA GACCCAGGCC TGGTCTCTGG CTACTGTGCT GGAGGTGCTC 
CATGATCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl ( XM_023334838.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023190606.1 Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X1, mRNA.

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CloneID	OXi38998
Clone ID Related Accession (Same CDS sequence)	XM_023334838.1
Accession Version	XM_023334838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036448.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTCT CTTTGGAGAC CAGGGTTCCC TCCAAGTTCA GGAGAACTCC CATGATGCTG 
CCTCAGCACC TCAAGCAAAT CAGAGTCCTC ATGCTTAACG GAAAGGAGAA TTTAGAGAGA 
ACTTTGTTCA GACTTGAACA AGGTTTTGAG CTCCAGTTCC GCCTGGGTCC AAGCCTTCAA 
GGAAGGAGAG TCATAGTTCA CACCAACTAC CCACTTGATG GACAGAAGTT CATTCGAAAC 
AACTTCCGTG TTTTAGCCTG GAACTACCCA ACAGGAAGGG AGGATGACTC TGATAAGTAC 
TGCTCCCTAG AGCTCAAGAT TGCTGGATCC TACCAGTACT ACTTTGGATA TGCAGGTGTG 
GAGAGGTTAG GAGGAGGCTA CATCGTCGTT GATCCTATTC TTCATGTTGG AGCAGACAAC 
CATGTCCTTC TGCTGGACTG TGTCACCATC CAGACGTACT TGTCCAAATG TCTGGGACGC 
CTTGATGACT GGCCAGACAG ACTGAGAGTA GCCAAGGAGT CAGGTTACAA CATGATCCAC 
TTCACTCCTC TGCAGACTTT GGGAGAGTCT AGGTCCTGTT ACTCCTTAGC TGACCAGCTG 
ACCTTTAGCC TGGAGTTCAG CCCAGATGGC AAGAGTTGCA CCTGGGAAGA TGTGGGAAGA 
CTGGTGGAAA AACTGAGGAT GGAATGGAAT ATGGTATCAA TCACTGATGT GGTGTATAAT 
CACACTGCTG CAAACAGTGT GTGGATAAAG GAACATCCAG AGTGTGGCTA CAACCTGATG 
AACTCTCCTC ATCTGCGTCC TGCCTGGGTC TTGGACAGAG CTATCTGGCA TTTAACCACT 
AGGGTAGCAG ATGGACGCTA CAAAGAGAAG GGACTTCCTT CTGACATTAC CAACGAGTGC 
CACCTAAATG CTCTCCGCAG GGTGTTTTGG GAGGACATGT TTCCTCAAAT CAAACTTTGG 
GAGTTCTACC AGGTCAACGT GGAAAATGCT GTGGAGCAGT TCAGAATCCT GTTGCAAAAT 
GGCACAAAGC CAGATCACAG CAAGACTGAA GGAAATAAAG GATTAGAGAT CATTCAAGAC 
CTCCAGTGCT GTCGCTATGG CAACAGAGTA AACATGGACT CAGCTTTGGA GACATTTGTA 
CCTCACAGCT CCTCTCCACA GCACATTGAA GAGTGCTGTG GTTGGCTGAA ACAGAAGTTG 
ACTGAGATGA ATGAAGAACA GTACCACATC ATACACCAGC ATCAGGAACA GGCAGTCAAC 
TGCATTGTGG AGAACATAGT GTATGAACGT CTAGCTGACC ACGGTCCTAA ACTGGGTCCA 
GTTACCAGGA AAGAGCCTCT TGTCACCAGG TATTTCACCT TCCCCTTTGA GGAAATGACT 
TTAGAGCAGG AGATGGAGTT TTTAAACCAA CCAGATAAGA TGTGCCACTT CTTGGCTCAC 
AACGGCTGGG TGATGGGTGA TGATCCATTG CGCAACTTTG CTGAACCAGG ATCCAATGTG 
TACCTGCGTC GGGAGCTGAT CTGCTGGGGG GACAGTGTCA AACTCCGCTA TGGGAAAGGA 
CCTGAGGATT GCCCCTACCT GTGGGCGCAC ATGCAGAAAT ACACTGAAAT TACTGCCAAA 
TATTTCCACG GAGTCCGATT GGACAACTGC CACTCTACGC CCTTGCATGT TGCTGAGTTC 
ATGTTGGATG TAGCCAGAAG CCAGTGTCCT AACCTGTACG TGGTGGCTGA ACTGTTCACT 
GGCAGCGAGG AGCTGGATAA CATCTTTGTT ACCAGACTAG GGATTACATC CCTCATACGA 
GAGGCCATGT CCGCCAGAGA CAGCCATGAA GAGGGCCGGC TGGTCTACCG TTACGGTGGA 
GAGCCGGTCG GAGCCTTTGT TCAGCCCAGC CTGCGTCCTC TCATGCCTTC TATTGCTCAC 
GCCATGTTCT TGGATGTGAC CCATGACAAC GAGTGTCCCA TTGAGCTGCG GTCAGCTCTC 
GACTCGTTGC CCAGTTCTGC CATCGTCTCC ATGGCCTGCT GTGCTACTGG CTCCACCAGA 
GGATATGATG AGCTGGTCCC ACATCAGATA TCTGTGGTGA AGGAGGAGCG TTTTTATCCC 
AAGTGGAATC CCTCTTCAGC TCCCACCTCT ACTGGTGAAG TTGGCTTTAA GAGCGGCATC 
ATCACTGGGA AGCTGGCTCT CAACAAGCTG CACCAGGAGC TGGCTGCCCA GGGCTTTTCA 
CAGGTGTATG TGGACCAGGT CGATGCAGAT ATTGTTGCAG TTACTCGTCA CTGCCCCAGC 
ACACACCAGT CTGTGGTAAC AGTGAGCAGG ACAGCCTTCA AGAACCCCAA AACTCATCAG 
TACAGCAACA ATGTCCCACC TATGTTCATA CCTGGCAAGA TTGAAGAAAT TGTACTGGAG 
GCAAGGACAG TGGAGAGAGA GGCTAGGAGT TACAAGAAGG ATGAGAAATA CATAAACGGC 
ATGCCAGAAT ACACAGTGGA AATAAAAGAG CATATACCGA TGGAGGAGAG TGCAGTGGTG 
AAACATGCTG GAGTGACATC TAAAGGCCGC TCAGAGTTTG TTCAAGAAAT TCAGTTCCAG 
AAATTGACCC CTGGTAGCAT CATTGCCTTC AGAGTCAGTT TGGATCCCAA GGCACAGAAG 
CTGGTGGGCG TGTTGAGGTT TTATCTGTCC CAGTTCAGTC CAAAGTACAG GAGGGGCAGT 
GTACCTGATG AGAACCCACC AGAAGTGCTG CAGAAACCCC TTACGCAGTT AATGTCTAAA 
CTGACGCTAG CCGACATGAA CATCTTGCTG TTTCGCTGCG AAGCAGAAGA ACAAGAGGAC 
GGTGGAGGCT GTTACAGTAT CCCAGGCTGG GAGTGCCTGA AGTATGCAGG ACTACAAGGT 
CTGATGTCTG TATTGGCGGA TATCCGTCCC ACTAATGACC TTGGCCATCC CCTGTGTGCT 
AACCTCAGAG AAGGAGACTG GCTCATAGAC TTTGTCTCCA ATAGGCTGAA GCACAGAGAT 
GGACCTCTGG CACAGGTGGG TCAGTGGTTG GGTGCCATTT TTGACTACCT GAGGCACTTT 
CCTCGATATC TCATCCCCTG TTACTTTGAC GCCATCTTGG TGTCAACCTA CACAACAGCT 
CTGGATGCCA CGTTCAAACT CATGTCGAGC TTCATTGAGC GAGGTTCCAC GTTTGTCCGT 
CACCTGGCTC TCGGCTCGGT TCAGATGTGT GGAGTTGGAC GTTTTCCGGC TCTGCCTCCC 
ATTTCAACAA AAGTGCAGGA TGTCCCATGC CGCATCAGTC CTATCACTGG GGAAAAGGAG 
CAGTGTTGTG TCTCACTGGC AGCAGGTCTT CCTCATTTTT CTGCTGGGAT TTTCCGTTGT 
TGGGGTAGAG ATACTTTCAT CGCTCTCAGA GGACTTATGC TTATAACAGG GAGACACACT 
GAGGCACGCA ACATCATCCT GGCTTTTGCT GGAACCCTGC GTCACGGTTT AATCCCCAAC 
TTGCTGGGAG AAGGTCACAA TGCCAGATAC AACTGCAGGG ATGCTGTGTG GTGGTGGCTG 
CAGTGCATTC AGGACTATAC CAATCTCGTT CCTGAGGGAC ATGAAATTCT GCATTGCCCT 
GTCACCCGCA TGTACCCCAC AGACGACTGT GAGCCTTGCA AACCAGGGGA GGAACAGCCA 
TTGTATGATG TCATCCAGGA GGCACTGCAG CGCCATCTGG AGGGTATTTC CTTCAGAGAG 
AGGAATGCTG GACCCAAGAT AGACAGGAAC ATGAGAGATG AAGGGTTCAA TGTGGTTGCT 
AAAGTGGACC CAGCAACAGG TTTTGTTACC GGAGGAAACC GCTTCAACTG TGGCACTTGG 
ATGGATAAAA TGGGGGAGAG TGACAGAGCG AGGAACAAGG GGATGCCTGC AACTCCAAGA 
GACGGCGCGG CGGTGGAGAT CGTTGGTCTG AGTAAATCAG CAGTACGTTG GCTTGTGGAG 
CTGCAGTCTA AAGAACTGTT CCCCTATGAT GGAGTTAAGG TGCTTAGAGA TGGAACAGAT 
GTGTTCATCT TGTACACTGA GTGGAACAAG CAGCTTCAGC AGTCCTTTGA AGTTGAATTC 
TGGGTGTCTG GGGACTCACA GGACCCCAAT GAGAAGCACC CTGATTTGGT GCATAAAAAG 
GGAATTTATA AAGATAGTTA TGGAGCTTCC AATCCCTGGT GTGACTACCA GCTGAGGCCC 
AACTTCACCA TCGCAATGGT GGTGGCTCCA GAGCTGTTCA CGTTAGAAAG AGCATGGAAG 
GCTTTGGAAA TAGCTCAGAA GAAACTCTTG GGACCACTGG GAATGAAAAC TCTTGACCCA 
GATGACATGG TCTACTGTGG TGTTTACAAT AATGCACTGG ACAGCGACAA CTACAACCTG 
GCCAAGGGCT TTAACTACCA CCAAGGACCT GAGTGGCTGT GGCCTGTTGG CTATTTTCTC 
CGTGCTAAGC TCTACTTTGC CAAGAAGTTA GGGGAGGAGG CCTACTCCAA GACCGTCATT 
CTAGTGAAAA ATGTTCTGTC ACGACATTAT ACCCACTTGG AGAGGTCTCT ATGGAAAGGC 
TTACCAGAGC TGACCAATGA GAATGGCCAG CATTGTCCCT TCAGCTGTGA GACCCAGGCC 
TGGTCTCTGG CTACTGTGCT GGAGGTGCTC CATGATCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023190606.1
CDS	96..4757
Translation	MPVSLETRVPSKFRRTPMMLPQHLKQIRVLMLNGKENLERTLFRLEQGFELQFRLGPSLQGRRVIVHTNYPLDGQKFIRNNFRVLAWNYPTGREDDSDKYCSLELKIAGSYQYYFGYAGVERLGGGYIVVDPILHVGADNHVLLLDCVTIQTYLSKCLGRLDDWPDRLRVAKESGYNMIHFTPLQTLGESRSCYSLADQLTFSLEFSPDGKSCTWEDVGRLVEKLRMEWNMVSITDVVYNHTAANSVWIKEHPECGYNLMNSPHLRPAWVLDRAIWHLTTRVADGRYKEKGLPSDITNECHLNALRRVFWEDMFPQIKLWEFYQVNVENAVEQFRILLQNGTKPDHSKTEGNKGLEIIQDLQCCRYGNRVNMDSALETFVPHSSSPQHIEECCGWLKQKLTEMNEEQYHIIHQHQEQAVNCIVENIVYERLADHGPKLGPVTRKEPLVTRYFTFPFEEMTLEQEMEFLNQPDKMCHFLAHNGWVMGDDPLRNFAEPGSNVYLRRELICWGDSVKLRYGKGPEDCPYLWAHMQKYTEITAKYFHGVRLDNCHSTPLHVAEFMLDVARSQCPNLYVVAELFTGSEELDNIFVTRLGITSLIREAMSARDSHEEGRLVYRYGGEPVGAFVQPSLRPLMPSIAHAMFLDVTHDNECPIELRSALDSLPSSAIVSMACCATGSTRGYDELVPHQISVVKEERFYPKWNPSSAPTSTGEVGFKSGIITGKLALNKLHQELAAQGFSQVYVDQVDADIVAVTRHCPSTHQSVVTVSRTAFKNPKTHQYSNNVPPMFIPGKIEEIVLEARTVEREARSYKKDEKYINGMPEYTVEIKEHIPMEESAVVKHAGVTSKGRSEFVQEIQFQKLTPGSIIAFRVSLDPKAQKLVGVLRFYLSQFSPKYRRGSVPDENPPEVLQKPLTQLMSKLTLADMNILLFRCEAEEQEDGGGCYSIPGWECLKYAGLQGLMSVLADIRPTNDLGHPLCANLREGDWLIDFVSNRLKHRDGPLAQVGQWLGAIFDYLRHFPRYLIPCYFDAILVSTYTTALDATFKLMSSFIERGSTFVRHLALGSVQMCGVGRFPALPPISTKVQDVPCRISPITGEKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLMLITGRHTEARNIILAFAGTLRHGLIPNLLGEGHNARYNCRDAVWWWLQCIQDYTNLVPEGHEILHCPVTRMYPTDDCEPCKPGEEQPLYDVIQEALQRHLEGISFRERNAGPKIDRNMRDEGFNVVAKVDPATGFVTGGNRFNCGTWMDKMGESDRARNKGMPATPRDGAAVEIVGLSKSAVRWLVELQSKELFPYDGVKVLRDGTDVFILYTEWNKQLQQSFEVEFWVSGDSQDPNEKHPDLVHKKGIYKDSYGASNPWCDYQLRPNFTIAMVVAPELFTLERAWKALEIAQKKLLGPLGMKTLDPDDMVYCGVYNNALDSDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFAKKLGEEAYSKTVILVKNVLSRHYTHLERSLWKGLPELTNENGQHCPFSCETQAWSLATVLEVLHDL

Target ORF information:

RefSeq Version	XM_023334838.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023334838.1

ORF Insert Sequence:

ATGCCTGTCT CTTTGGAGAC CAGGGTTCCC TCCAAGTTCA GGAGAACTCC CATGATGCTG 
CCTCAGCACC TCAAGCAAAT CAGAGTCCTC ATGCTTAACG GAAAGGAGAA TTTAGAGAGA 
ACTTTGTTCA GACTTGAACA AGGTTTTGAG CTCCAGTTCC GCCTGGGTCC AAGCCTTCAA 
GGAAGGAGAG TCATAGTTCA CACCAACTAC CCACTTGATG GACAGAAGTT CATTCGAAAC 
AACTTCCGTG TTTTAGCCTG GAACTACCCA ACAGGAAGGG AGGATGACTC TGATAAGTAC 
TGCTCCCTAG AGCTCAAGAT TGCTGGATCC TACCAGTACT ACTTTGGATA TGCAGGTGTG 
GAGAGGTTAG GAGGAGGCTA CATCGTCGTT GATCCTATTC TTCATGTTGG AGCAGACAAC 
CATGTCCTTC TGCTGGACTG TGTCACCATC CAGACGTACT TGTCCAAATG TCTGGGACGC 
CTTGATGACT GGCCAGACAG ACTGAGAGTA GCCAAGGAGT CAGGTTACAA CATGATCCAC 
TTCACTCCTC TGCAGACTTT GGGAGAGTCT AGGTCCTGTT ACTCCTTAGC TGACCAGCTG 
ACCTTTAGCC TGGAGTTCAG CCCAGATGGC AAGAGTTGCA CCTGGGAAGA TGTGGGAAGA 
CTGGTGGAAA AACTGAGGAT GGAATGGAAT ATGGTATCAA TCACTGATGT GGTGTATAAT 
CACACTGCTG CAAACAGTGT GTGGATAAAG GAACATCCAG AGTGTGGCTA CAACCTGATG 
AACTCTCCTC ATCTGCGTCC TGCCTGGGTC TTGGACAGAG CTATCTGGCA TTTAACCACT 
AGGGTAGCAG ATGGACGCTA CAAAGAGAAG GGACTTCCTT CTGACATTAC CAACGAGTGC 
CACCTAAATG CTCTCCGCAG GGTGTTTTGG GAGGACATGT TTCCTCAAAT CAAACTTTGG 
GAGTTCTACC AGGTCAACGT GGAAAATGCT GTGGAGCAGT TCAGAATCCT GTTGCAAAAT 
GGCACAAAGC CAGATCACAG CAAGACTGAA GGAAATAAAG GATTAGAGAT CATTCAAGAC 
CTCCAGTGCT GTCGCTATGG CAACAGAGTA AACATGGACT CAGCTTTGGA GACATTTGTA 
CCTCACAGCT CCTCTCCACA GCACATTGAA GAGTGCTGTG GTTGGCTGAA ACAGAAGTTG 
ACTGAGATGA ATGAAGAACA GTACCACATC ATACACCAGC ATCAGGAACA GGCAGTCAAC 
TGCATTGTGG AGAACATAGT GTATGAACGT CTAGCTGACC ACGGTCCTAA ACTGGGTCCA 
GTTACCAGGA AAGAGCCTCT TGTCACCAGG TATTTCACCT TCCCCTTTGA GGAAATGACT 
TTAGAGCAGG AGATGGAGTT TTTAAACCAA CCAGATAAGA TGTGCCACTT CTTGGCTCAC 
AACGGCTGGG TGATGGGTGA TGATCCATTG CGCAACTTTG CTGAACCAGG ATCCAATGTG 
TACCTGCGTC GGGAGCTGAT CTGCTGGGGG GACAGTGTCA AACTCCGCTA TGGGAAAGGA 
CCTGAGGATT GCCCCTACCT GTGGGCGCAC ATGCAGAAAT ACACTGAAAT TACTGCCAAA 
TATTTCCACG GAGTCCGATT GGACAACTGC CACTCTACGC CCTTGCATGT TGCTGAGTTC 
ATGTTGGATG TAGCCAGAAG CCAGTGTCCT AACCTGTACG TGGTGGCTGA ACTGTTCACT 
GGCAGCGAGG AGCTGGATAA CATCTTTGTT ACCAGACTAG GGATTACATC CCTCATACGA 
GAGGCCATGT CCGCCAGAGA CAGCCATGAA GAGGGCCGGC TGGTCTACCG TTACGGTGGA 
GAGCCGGTCG GAGCCTTTGT TCAGCCCAGC CTGCGTCCTC TCATGCCTTC TATTGCTCAC 
GCCATGTTCT TGGATGTGAC CCATGACAAC GAGTGTCCCA TTGAGCTGCG GTCAGCTCTC 
GACTCGTTGC CCAGTTCTGC CATCGTCTCC ATGGCCTGCT GTGCTACTGG CTCCACCAGA 
GGATATGATG AGCTGGTCCC ACATCAGATA TCTGTGGTGA AGGAGGAGCG TTTTTATCCC 
AAGTGGAATC CCTCTTCAGC TCCCACCTCT ACTGGTGAAG TTGGCTTTAA GAGCGGCATC 
ATCACTGGGA AGCTGGCTCT CAACAAGCTG CACCAGGAGC TGGCTGCCCA GGGCTTTTCA 
CAGGTGTATG TGGACCAGGT CGATGCAGAT ATTGTTGCAG TTACTCGTCA CTGCCCCAGC 
ACACACCAGT CTGTGGTAAC AGTGAGCAGG ACAGCCTTCA AGAACCCCAA AACTCATCAG 
TACAGCAACA ATGTCCCACC TATGTTCATA CCTGGCAAGA TTGAAGAAAT TGTACTGGAG 
GCAAGGACAG TGGAGAGAGA GGCTAGGAGT TACAAGAAGG ATGAGAAATA CATAAACGGC 
ATGCCAGAAT ACACAGTGGA AATAAAAGAG CATATACCGA TGGAGGAGAG TGCAGTGGTG 
AAACATGCTG GAGTGACATC TAAAGGCCGC TCAGAGTTTG TTCAAGAAAT TCAGTTCCAG 
AAATTGACCC CTGGTAGCAT CATTGCCTTC AGAGTCAGTT TGGATCCCAA GGCACAGAAG 
CTGGTGGGCG TGTTGAGGTT TTATCTGTCC CAGTTCAGTC CAAAGTACAG GAGGGGCAGT 
GTACCTGATG AGAACCCACC AGAAGTGCTG CAGAAACCCC TTACGCAGTT AATGTCTAAA 
CTGACGCTAG CCGACATGAA CATCTTGCTG TTTCGCTGCG AAGCAGAAGA ACAAGAGGAC 
GGTGGAGGCT GTTACAGTAT CCCAGGCTGG GAGTGCCTGA AGTATGCAGG ACTACAAGGT 
CTGATGTCTG TATTGGCGGA TATCCGTCCC ACTAATGACC TTGGCCATCC CCTGTGTGCT 
AACCTCAGAG AAGGAGACTG GCTCATAGAC TTTGTCTCCA ATAGGCTGAA GCACAGAGAT 
GGACCTCTGG CACAGGTGGG TCAGTGGTTG GGTGCCATTT TTGACTACCT GAGGCACTTT 
CCTCGATATC TCATCCCCTG TTACTTTGAC GCCATCTTGG TGTCAACCTA CACAACAGCT 
CTGGATGCCA CGTTCAAACT CATGTCGAGC TTCATTGAGC GAGGTTCCAC GTTTGTCCGT 
CACCTGGCTC TCGGCTCGGT TCAGATGTGT GGAGTTGGAC GTTTTCCGGC TCTGCCTCCC 
ATTTCAACAA AAGTGCAGGA TGTCCCATGC CGCATCAGTC CTATCACTGG GGAAAAGGAG 
CAGTGTTGTG TCTCACTGGC AGCAGGTCTT CCTCATTTTT CTGCTGGGAT TTTCCGTTGT 
TGGGGTAGAG ATACTTTCAT CGCTCTCAGA GGACTTATGC TTATAACAGG GAGACACACT 
GAGGCACGCA ACATCATCCT GGCTTTTGCT GGAACCCTGC GTCACGGTTT AATCCCCAAC 
TTGCTGGGAG AAGGTCACAA TGCCAGATAC AACTGCAGGG ATGCTGTGTG GTGGTGGCTG 
CAGTGCATTC AGGACTATAC CAATCTCGTT CCTGAGGGAC ATGAAATTCT GCATTGCCCT 
GTCACCCGCA TGTACCCCAC AGACGACTGT GAGCCTTGCA AACCAGGGGA GGAACAGCCA 
TTGTATGATG TCATCCAGGA GGCACTGCAG CGCCATCTGG AGGGTATTTC CTTCAGAGAG 
AGGAATGCTG GACCCAAGAT AGACAGGAAC ATGAGAGATG AAGGGTTCAA TGTGGTTGCT 
AAAGTGGACC CAGCAACAGG TTTTGTTACC GGAGGAAACC GCTTCAACTG TGGCACTTGG 
ATGGATAAAA TGGGGGAGAG TGACAGAGCG AGGAACAAGG GGATGCCTGC AACTCCAAGA 
GACGGCGCGG CGGTGGAGAT CGTTGGTCTG AGTAAATCAG CAGTACGTTG GCTTGTGGAG 
CTGCAGTCTA AAGAACTGTT CCCCTATGAT GGAGTTAAGG TGCTTAGAGA TGGAACAGAT 
GTGTTCATCT TGTACACTGA GTGGAACAAG CAGCTTCAGC AGTCCTTTGA AGTTGAATTC 
TGGGTGTCTG GGGACTCACA GGACCCCAAT GAGAAGCACC CTGATTTGGT GCATAAAAAG 
GGAATTTATA AAGATAGTTA TGGAGCTTCC AATCCCTGGT GTGACTACCA GCTGAGGCCC 
AACTTCACCA TCGCAATGGT GGTGGCTCCA GAGCTGTTCA CGTTAGAAAG AGCATGGAAG 
GCTTTGGAAA TAGCTCAGAA GAAACTCTTG GGACCACTGG GAATGAAAAC TCTTGACCCA 
GATGACATGG TCTACTGTGG TGTTTACAAT AATGCACTGG ACAGCGACAA CTACAACCTG 
GCCAAGGGCT TTAACTACCA CCAAGGACCT GAGTGGCTGT GGCCTGTTGG CTATTTTCTC 
CGTGCTAAGC TCTACTTTGC CAAGAAGTTA GGGGAGGAGG CCTACTCCAA GACCGTCATT 
CTAGTGAAAA ATGTTCTGTC ACGACATTAT ACCCACTTGG AGAGGTCTCT ATGGAAAGGC 
TTACCAGAGC TGACCAATGA GAATGGCCAG CATTGTCCCT TCAGCTGTGA GACCCAGGCC 
TGGTCTCTGG CTACTGTGCT GGAGGTGCTC CATGATCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl ( XM_023334840.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023190608.1 Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X3, mRNA.

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CloneID	OXi38999
Clone ID Related Accession (Same CDS sequence)	XM_023334840.1 , XM_023334839.1
Accession Version	XM_023334840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036448.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGC CTCAGCACCT CAAGCAAATC AGAGTCCTCA TGCTTAACGG AAAGGAGAAT 
TTAGAGAGAA CTTTGTTCAG ACTTGAACAA GGTTTTGAGC TCCAGTTCCG CCTGGGTCCA 
AGCCTTCAAG GAAGGAGAGT CATAGTTCAC ACCAACTACC CACTTGATGG ACAGAAGTTC 
ATTCGAAACA ACTTCCGTGT TTTAGCCTGG AACTACCCAA CAGGAAGGGA GGATGACTCT 
GATAAGTACT GCTCCCTAGA GCTCAAGATT GCTGGATCCT ACCAGTACTA CTTTGGATAT 
GCAGGTGTGG AGAGGTTAGG AGGAGGCTAC ATCGTCGTTG ATCCTATTCT TCATGTTGGA 
GCAGACAACC ATGTCCTTCT GCTGGACTGT GTCACCATCC AGACGTACTT GTCCAAATGT 
CTGGGACGCC TTGATGACTG GCCAGACAGA CTGAGAGTAG CCAAGGAGTC AGGTTACAAC 
ATGATCCACT TCACTCCTCT GCAGACTTTG GGAGAGTCTA GGTCCTGTTA CTCCTTAGCT 
GACCAGCTGA CCTTTAGCCT GGAGTTCAGC CCAGATGGCA AGAGTTGCAC CTGGGAAGAT 
GTGGGAAGAC TGGTGGAAAA ACTGAGGATG GAATGGAATA TGGTATCAAT CACTGATGTG 
GTGTATAATC ACACTGCTGC AAACAGTGTG TGGATAAAGG AACATCCAGA GTGTGGCTAC 
AACCTGATGA ACTCTCCTCA TCTGCGTCCT GCCTGGGTCT TGGACAGAGC TATCTGGCAT 
TTAACCACTA GGGTAGCAGA TGGACGCTAC AAAGAGAAGG GACTTCCTTC TGACATTACC 
AACGAGTGCC ACCTAAATGC TCTCCGCAGG GTGTTTTGGG AGGACATGTT TCCTCAAATC 
AAACTTTGGG AGTTCTACCA GGTCAACGTG GAAAATGCTG TGGAGCAGTT CAGAATCCTG 
TTGCAAAATG GCACAAAGCC AGATCACAGC AAGACTGAAG GAAATAAAGG ATTAGAGATC 
ATTCAAGACC TCCAGTGCTG TCGCTATGGC AACAGAGTAA ACATGGACTC AGCTTTGGAG 
ACATTTGTAC CTCACAGCTC CTCTCCACAG CACATTGAAG AGTGCTGTGG TTGGCTGAAA 
CAGAAGTTGA CTGAGATGAA TGAAGAACAG TACCACATCA TACACCAGCA TCAGGAACAG 
GCAGTCAACT GCATTGTGGA GAACATAGTG TATGAACGTC TAGCTGACCA CGGTCCTAAA 
CTGGGTCCAG TTACCAGGAA AGAGCCTCTT GTCACCAGGT ATTTCACCTT CCCCTTTGAG 
GAAATGACTT TAGAGCAGGA GATGGAGTTT TTAAACCAAC CAGATAAGAT GTGCCACTTC 
TTGGCTCACA ACGGCTGGGT GATGGGTGAT GATCCATTGC GCAACTTTGC TGAACCAGGA 
TCCAATGTGT ACCTGCGTCG GGAGCTGATC TGCTGGGGGG ACAGTGTCAA ACTCCGCTAT 
GGGAAAGGAC CTGAGGATTG CCCCTACCTG TGGGCGCACA TGCAGAAATA CACTGAAATT 
ACTGCCAAAT ATTTCCACGG AGTCCGATTG GACAACTGCC ACTCTACGCC CTTGCATGTT 
GCTGAGTTCA TGTTGGATGT AGCCAGAAGC CAGTGTCCTA ACCTGTACGT GGTGGCTGAA 
CTGTTCACTG GCAGCGAGGA GCTGGATAAC ATCTTTGTTA CCAGACTAGG GATTACATCC 
CTCATACGAG AGGCCATGTC CGCCAGAGAC AGCCATGAAG AGGGCCGGCT GGTCTACCGT 
TACGGTGGAG AGCCGGTCGG AGCCTTTGTT CAGCCCAGCC TGCGTCCTCT CATGCCTTCT 
ATTGCTCACG CCATGTTCTT GGATGTGACC CATGACAACG AGTGTCCCAT TGAGCTGCGG 
TCAGCTCTCG ACTCGTTGCC CAGTTCTGCC ATCGTCTCCA TGGCCTGCTG TGCTACTGGC 
TCCACCAGAG GATATGATGA GCTGGTCCCA CATCAGATAT CTGTGGTGAA GGAGGAGCGT 
TTTTATCCCA AGTGGAATCC CTCTTCAGCT CCCACCTCTA CTGGTGAAGT TGGCTTTAAG 
AGCGGCATCA TCACTGGGAA GCTGGCTCTC AACAAGCTGC ACCAGGAGCT GGCTGCCCAG 
GGCTTTTCAC AGGTGTATGT GGACCAGGTC GATGCAGATA TTGTTGCAGT TACTCGTCAC 
TGCCCCAGCA CACACCAGTC TGTGGTAACA GTGAGCAGGA CAGCCTTCAA GAACCCCAAA 
ACTCATCAGT ACAGCAACAA TGTCCCACCT ATGTTCATAC CTGGCAAGAT TGAAGAAATT 
GTACTGGAGG CAAGGACAGT GGAGAGAGAG GCTAGGAGTT ACAAGAAGGA TGAGAAATAC 
ATAAACGGCA TGCCAGAATA CACAGTGGAA ATAAAAGAGC ATATACCGAT GGAGGAGAGT 
GCAGTGGTGA AACATGCTGG AGTGACATCT AAAGGCCGCT CAGAGTTTGT TCAAGAAATT 
CAGTTCCAGA AATTGACCCC TGGTAGCATC ATTGCCTTCA GAGTCAGTTT GGATCCCAAG 
GCACAGAAGC TGGTGGGCGT GTTGAGGTTT TATCTGTCCC AGTTCAGTCC AAAGTACAGG 
AGGGGCAGTG TACCTGATGA GAACCCACCA GAAGTGCTGC AGAAACCCCT TACGCAGTTA 
ATGTCTAAAC TGACGCTAGC CGACATGAAC ATCTTGCTGT TTCGCTGCGA AGCAGAAGAA 
CAAGAGGACG GTGGAGGCTG TTACAGTATC CCAGGCTGGG AGTGCCTGAA GTATGCAGGA 
CTACAAGGTC TGATGTCTGT ATTGGCGGAT ATCCGTCCCA CTAATGACCT TGGCCATCCC 
CTGTGTGCTA ACCTCAGAGA AGGAGACTGG CTCATAGACT TTGTCTCCAA TAGGCTGAAG 
CACAGAGATG GACCTCTGGC ACAGGTGGGT CAGTGGTTGG GTGCCATTTT TGACTACCTG 
AGGCACTTTC CTCGATATCT CATCCCCTGT TACTTTGACG CCATCTTGGT GTCAACCTAC 
ACAACAGCTC TGGATGCCAC GTTCAAACTC ATGTCGAGCT TCATTGAGCG AGGTTCCACG 
TTTGTCCGTC ACCTGGCTCT CGGCTCGGTT CAGATGTGTG GAGTTGGACG TTTTCCGGCT 
CTGCCTCCCA TTTCAACAAA AGTGCAGGAT GTCCCATGCC GCATCAGTCC TATCACTGGG 
GAAAAGGAGC AGTGTTGTGT CTCACTGGCA GCAGGTCTTC CTCATTTTTC TGCTGGGATT 
TTCCGTTGTT GGGGTAGAGA TACTTTCATC GCTCTCAGAG GACTTATGCT TATAACAGGG 
AGACACACTG AGGCACGCAA CATCATCCTG GCTTTTGCTG GAACCCTGCG TCACGGTTTA 
ATCCCCAACT TGCTGGGAGA AGGTCACAAT GCCAGATACA ACTGCAGGGA TGCTGTGTGG 
TGGTGGCTGC AGTGCATTCA GGACTATACC AATCTCGTTC CTGAGGGACA TGAAATTCTG 
CATTGCCCTG TCACCCGCAT GTACCCCACA GACGACTGTG AGCCTTGCAA ACCAGGGGAG 
GTTGAACAGC CATTGTATGA TGTCATCCAG GAGGCACTGC AGCGCCATCT GGAGGGTATT 
TCCTTCAGAG AGAGGAATGC TGGACCCAAG ATAGACAGGA ACATGAGAGA TGAAGGGTTC 
AATGTGGTTG CTAAAGTGGA CCCAGCAACA GGTTTTGTTA CCGGAGGAAA CCGCTTCAAC 
TGTGGCACTT GGATGGATAA AATGGGGGAG AGTGACAGAG CGAGGAACAA GGGGATGCCT 
GCAACTCCAA GAGACGGCGC GGCGGTGGAG ATCGTTGGTC TGAGTAAATC AGCAGTACGT 
TGGCTTGTGG AGCTGCAGTC TAAAGAACTG TTCCCCTATG ATGGAGTTAA GGTGCTTAGA 
GATGGAACAG ATGTGTTCAT CTTGTACACT GAGTGGAACA AGCAGCTTCA GCAGTCCTTT 
GAAGTTGAAT TCTGGGTGTC TGGGGACTCA CAGGACCCCA ATGAGAAGCA CCCTGATTTG 
GTGCATAAAA AGGGAATTTA TAAAGATAGT TATGGAGCTT CCAATCCCTG GTGTGACTAC 
CAGCTGAGGC CCAACTTCAC CATCGCAATG GTGGTGGCTC CAGAGCTGTT CACGTTAGAA 
AGAGCATGGA AGGCTTTGGA AATAGCTCAG AAGAAACTCT TGGGACCACT GGGAATGAAA 
ACTCTTGACC CAGATGACAT GGTCTACTGT GGTGTTTACA ATAATGCACT GGACAGCGAC 
AACTACAACC TGGCCAAGGG CTTTAACTAC CACCAAGGAC CTGAGTGGCT GTGGCCTGTT 
GGCTATTTTC TCCGTGCTAA GCTCTACTTT GCCAAGAAGT TAGGGGAGGA GGCCTACTCC 
AAGACCGTCA TTCTAGTGAA AAATGTTCTG TCACGACATT ATACCCACTT GGAGAGGTCT 
CTATGGAAAG GCTTACCAGA GCTGACCAAT GAGAATGGCC AGCATTGTCC CTTCAGCTGT 
GAGACCCAGG CCTGGTCTCT GGCTACTGTG CTGGAGGTGC TCCATGATCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023190608.1
CDS	182..4795
Translation	MMLPQHLKQIRVLMLNGKENLERTLFRLEQGFELQFRLGPSLQGRRVIVHTNYPLDGQKFIRNNFRVLAWNYPTGREDDSDKYCSLELKIAGSYQYYFGYAGVERLGGGYIVVDPILHVGADNHVLLLDCVTIQTYLSKCLGRLDDWPDRLRVAKESGYNMIHFTPLQTLGESRSCYSLADQLTFSLEFSPDGKSCTWEDVGRLVEKLRMEWNMVSITDVVYNHTAANSVWIKEHPECGYNLMNSPHLRPAWVLDRAIWHLTTRVADGRYKEKGLPSDITNECHLNALRRVFWEDMFPQIKLWEFYQVNVENAVEQFRILLQNGTKPDHSKTEGNKGLEIIQDLQCCRYGNRVNMDSALETFVPHSSSPQHIEECCGWLKQKLTEMNEEQYHIIHQHQEQAVNCIVENIVYERLADHGPKLGPVTRKEPLVTRYFTFPFEEMTLEQEMEFLNQPDKMCHFLAHNGWVMGDDPLRNFAEPGSNVYLRRELICWGDSVKLRYGKGPEDCPYLWAHMQKYTEITAKYFHGVRLDNCHSTPLHVAEFMLDVARSQCPNLYVVAELFTGSEELDNIFVTRLGITSLIREAMSARDSHEEGRLVYRYGGEPVGAFVQPSLRPLMPSIAHAMFLDVTHDNECPIELRSALDSLPSSAIVSMACCATGSTRGYDELVPHQISVVKEERFYPKWNPSSAPTSTGEVGFKSGIITGKLALNKLHQELAAQGFSQVYVDQVDADIVAVTRHCPSTHQSVVTVSRTAFKNPKTHQYSNNVPPMFIPGKIEEIVLEARTVEREARSYKKDEKYINGMPEYTVEIKEHIPMEESAVVKHAGVTSKGRSEFVQEIQFQKLTPGSIIAFRVSLDPKAQKLVGVLRFYLSQFSPKYRRGSVPDENPPEVLQKPLTQLMSKLTLADMNILLFRCEAEEQEDGGGCYSIPGWECLKYAGLQGLMSVLADIRPTNDLGHPLCANLREGDWLIDFVSNRLKHRDGPLAQVGQWLGAIFDYLRHFPRYLIPCYFDAILVSTYTTALDATFKLMSSFIERGSTFVRHLALGSVQMCGVGRFPALPPISTKVQDVPCRISPITGEKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLMLITGRHTEARNIILAFAGTLRHGLIPNLLGEGHNARYNCRDAVWWWLQCIQDYTNLVPEGHEILHCPVTRMYPTDDCEPCKPGEVEQPLYDVIQEALQRHLEGISFRERNAGPKIDRNMRDEGFNVVAKVDPATGFVTGGNRFNCGTWMDKMGESDRARNKGMPATPRDGAAVEIVGLSKSAVRWLVELQSKELFPYDGVKVLRDGTDVFILYTEWNKQLQQSFEVEFWVSGDSQDPNEKHPDLVHKKGIYKDSYGASNPWCDYQLRPNFTIAMVVAPELFTLERAWKALEIAQKKLLGPLGMKTLDPDDMVYCGVYNNALDSDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFAKKLGEEAYSKTVILVKNVLSRHYTHLERSLWKGLPELTNENGQHCPFSCETQAWSLATVLEVLHDL

Target ORF information:

RefSeq Version	XM_023334840.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023334840.1

ORF Insert Sequence:

ATGATGCTGC CTCAGCACCT CAAGCAAATC AGAGTCCTCA TGCTTAACGG AAAGGAGAAT 
TTAGAGAGAA CTTTGTTCAG ACTTGAACAA GGTTTTGAGC TCCAGTTCCG CCTGGGTCCA 
AGCCTTCAAG GAAGGAGAGT CATAGTTCAC ACCAACTACC CACTTGATGG ACAGAAGTTC 
ATTCGAAACA ACTTCCGTGT TTTAGCCTGG AACTACCCAA CAGGAAGGGA GGATGACTCT 
GATAAGTACT GCTCCCTAGA GCTCAAGATT GCTGGATCCT ACCAGTACTA CTTTGGATAT 
GCAGGTGTGG AGAGGTTAGG AGGAGGCTAC ATCGTCGTTG ATCCTATTCT TCATGTTGGA 
GCAGACAACC ATGTCCTTCT GCTGGACTGT GTCACCATCC AGACGTACTT GTCCAAATGT 
CTGGGACGCC TTGATGACTG GCCAGACAGA CTGAGAGTAG CCAAGGAGTC AGGTTACAAC 
ATGATCCACT TCACTCCTCT GCAGACTTTG GGAGAGTCTA GGTCCTGTTA CTCCTTAGCT 
GACCAGCTGA CCTTTAGCCT GGAGTTCAGC CCAGATGGCA AGAGTTGCAC CTGGGAAGAT 
GTGGGAAGAC TGGTGGAAAA ACTGAGGATG GAATGGAATA TGGTATCAAT CACTGATGTG 
GTGTATAATC ACACTGCTGC AAACAGTGTG TGGATAAAGG AACATCCAGA GTGTGGCTAC 
AACCTGATGA ACTCTCCTCA TCTGCGTCCT GCCTGGGTCT TGGACAGAGC TATCTGGCAT 
TTAACCACTA GGGTAGCAGA TGGACGCTAC AAAGAGAAGG GACTTCCTTC TGACATTACC 
AACGAGTGCC ACCTAAATGC TCTCCGCAGG GTGTTTTGGG AGGACATGTT TCCTCAAATC 
AAACTTTGGG AGTTCTACCA GGTCAACGTG GAAAATGCTG TGGAGCAGTT CAGAATCCTG 
TTGCAAAATG GCACAAAGCC AGATCACAGC AAGACTGAAG GAAATAAAGG ATTAGAGATC 
ATTCAAGACC TCCAGTGCTG TCGCTATGGC AACAGAGTAA ACATGGACTC AGCTTTGGAG 
ACATTTGTAC CTCACAGCTC CTCTCCACAG CACATTGAAG AGTGCTGTGG TTGGCTGAAA 
CAGAAGTTGA CTGAGATGAA TGAAGAACAG TACCACATCA TACACCAGCA TCAGGAACAG 
GCAGTCAACT GCATTGTGGA GAACATAGTG TATGAACGTC TAGCTGACCA CGGTCCTAAA 
CTGGGTCCAG TTACCAGGAA AGAGCCTCTT GTCACCAGGT ATTTCACCTT CCCCTTTGAG 
GAAATGACTT TAGAGCAGGA GATGGAGTTT TTAAACCAAC CAGATAAGAT GTGCCACTTC 
TTGGCTCACA ACGGCTGGGT GATGGGTGAT GATCCATTGC GCAACTTTGC TGAACCAGGA 
TCCAATGTGT ACCTGCGTCG GGAGCTGATC TGCTGGGGGG ACAGTGTCAA ACTCCGCTAT 
GGGAAAGGAC CTGAGGATTG CCCCTACCTG TGGGCGCACA TGCAGAAATA CACTGAAATT 
ACTGCCAAAT ATTTCCACGG AGTCCGATTG GACAACTGCC ACTCTACGCC CTTGCATGTT 
GCTGAGTTCA TGTTGGATGT AGCCAGAAGC CAGTGTCCTA ACCTGTACGT GGTGGCTGAA 
CTGTTCACTG GCAGCGAGGA GCTGGATAAC ATCTTTGTTA CCAGACTAGG GATTACATCC 
CTCATACGAG AGGCCATGTC CGCCAGAGAC AGCCATGAAG AGGGCCGGCT GGTCTACCGT 
TACGGTGGAG AGCCGGTCGG AGCCTTTGTT CAGCCCAGCC TGCGTCCTCT CATGCCTTCT 
ATTGCTCACG CCATGTTCTT GGATGTGACC CATGACAACG AGTGTCCCAT TGAGCTGCGG 
TCAGCTCTCG ACTCGTTGCC CAGTTCTGCC ATCGTCTCCA TGGCCTGCTG TGCTACTGGC 
TCCACCAGAG GATATGATGA GCTGGTCCCA CATCAGATAT CTGTGGTGAA GGAGGAGCGT 
TTTTATCCCA AGTGGAATCC CTCTTCAGCT CCCACCTCTA CTGGTGAAGT TGGCTTTAAG 
AGCGGCATCA TCACTGGGAA GCTGGCTCTC AACAAGCTGC ACCAGGAGCT GGCTGCCCAG 
GGCTTTTCAC AGGTGTATGT GGACCAGGTC GATGCAGATA TTGTTGCAGT TACTCGTCAC 
TGCCCCAGCA CACACCAGTC TGTGGTAACA GTGAGCAGGA CAGCCTTCAA GAACCCCAAA 
ACTCATCAGT ACAGCAACAA TGTCCCACCT ATGTTCATAC CTGGCAAGAT TGAAGAAATT 
GTACTGGAGG CAAGGACAGT GGAGAGAGAG GCTAGGAGTT ACAAGAAGGA TGAGAAATAC 
ATAAACGGCA TGCCAGAATA CACAGTGGAA ATAAAAGAGC ATATACCGAT GGAGGAGAGT 
GCAGTGGTGA AACATGCTGG AGTGACATCT AAAGGCCGCT CAGAGTTTGT TCAAGAAATT 
CAGTTCCAGA AATTGACCCC TGGTAGCATC ATTGCCTTCA GAGTCAGTTT GGATCCCAAG 
GCACAGAAGC TGGTGGGCGT GTTGAGGTTT TATCTGTCCC AGTTCAGTCC AAAGTACAGG 
AGGGGCAGTG TACCTGATGA GAACCCACCA GAAGTGCTGC AGAAACCCCT TACGCAGTTA 
ATGTCTAAAC TGACGCTAGC CGACATGAAC ATCTTGCTGT TTCGCTGCGA AGCAGAAGAA 
CAAGAGGACG GTGGAGGCTG TTACAGTATC CCAGGCTGGG AGTGCCTGAA GTATGCAGGA 
CTACAAGGTC TGATGTCTGT ATTGGCGGAT ATCCGTCCCA CTAATGACCT TGGCCATCCC 
CTGTGTGCTA ACCTCAGAGA AGGAGACTGG CTCATAGACT TTGTCTCCAA TAGGCTGAAG 
CACAGAGATG GACCTCTGGC ACAGGTGGGT CAGTGGTTGG GTGCCATTTT TGACTACCTG 
AGGCACTTTC CTCGATATCT CATCCCCTGT TACTTTGACG CCATCTTGGT GTCAACCTAC 
ACAACAGCTC TGGATGCCAC GTTCAAACTC ATGTCGAGCT TCATTGAGCG AGGTTCCACG 
TTTGTCCGTC ACCTGGCTCT CGGCTCGGTT CAGATGTGTG GAGTTGGACG TTTTCCGGCT 
CTGCCTCCCA TTTCAACAAA AGTGCAGGAT GTCCCATGCC GCATCAGTCC TATCACTGGG 
GAAAAGGAGC AGTGTTGTGT CTCACTGGCA GCAGGTCTTC CTCATTTTTC TGCTGGGATT 
TTCCGTTGTT GGGGTAGAGA TACTTTCATC GCTCTCAGAG GACTTATGCT TATAACAGGG 
AGACACACTG AGGCACGCAA CATCATCCTG GCTTTTGCTG GAACCCTGCG TCACGGTTTA 
ATCCCCAACT TGCTGGGAGA AGGTCACAAT GCCAGATACA ACTGCAGGGA TGCTGTGTGG 
TGGTGGCTGC AGTGCATTCA GGACTATACC AATCTCGTTC CTGAGGGACA TGAAATTCTG 
CATTGCCCTG TCACCCGCAT GTACCCCACA GACGACTGTG AGCCTTGCAA ACCAGGGGAG 
GTTGAACAGC CATTGTATGA TGTCATCCAG GAGGCACTGC AGCGCCATCT GGAGGGTATT 
TCCTTCAGAG AGAGGAATGC TGGACCCAAG ATAGACAGGA ACATGAGAGA TGAAGGGTTC 
AATGTGGTTG CTAAAGTGGA CCCAGCAACA GGTTTTGTTA CCGGAGGAAA CCGCTTCAAC 
TGTGGCACTT GGATGGATAA AATGGGGGAG AGTGACAGAG CGAGGAACAA GGGGATGCCT 
GCAACTCCAA GAGACGGCGC GGCGGTGGAG ATCGTTGGTC TGAGTAAATC AGCAGTACGT 
TGGCTTGTGG AGCTGCAGTC TAAAGAACTG TTCCCCTATG ATGGAGTTAA GGTGCTTAGA 
GATGGAACAG ATGTGTTCAT CTTGTACACT GAGTGGAACA AGCAGCTTCA GCAGTCCTTT 
GAAGTTGAAT TCTGGGTGTC TGGGGACTCA CAGGACCCCA ATGAGAAGCA CCCTGATTTG 
GTGCATAAAA AGGGAATTTA TAAAGATAGT TATGGAGCTT CCAATCCCTG GTGTGACTAC 
CAGCTGAGGC CCAACTTCAC CATCGCAATG GTGGTGGCTC CAGAGCTGTT CACGTTAGAA 
AGAGCATGGA AGGCTTTGGA AATAGCTCAG AAGAAACTCT TGGGACCACT GGGAATGAAA 
ACTCTTGACC CAGATGACAT GGTCTACTGT GGTGTTTACA ATAATGCACT GGACAGCGAC 
AACTACAACC TGGCCAAGGG CTTTAACTAC CACCAAGGAC CTGAGTGGCT GTGGCCTGTT 
GGCTATTTTC TCCGTGCTAA GCTCTACTTT GCCAAGAAGT TAGGGGAGGA GGCCTACTCC 
AAGACCGTCA TTCTAGTGAA AAATGTTCTG TCACGACATT ATACCCACTT GGAGAGGTCT 
CTATGGAAAG GCTTACCAGA GCTGACCAAT GAGAATGGCC AGCATTGTCC CTTCAGCTGT 
GAGACCCAGG CCTGGTCTCT GGCTACTGTG CTGGAGGTGC TCCATGATCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl ( XM_023334839.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023190607.1 Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X2, mRNA.

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CloneID	OXi38999
Clone ID Related Accession (Same CDS sequence)	XM_023334840.1 , XM_023334839.1
Accession Version	XM_023334839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036448.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGC CTCAGCACCT CAAGCAAATC AGAGTCCTCA TGCTTAACGG AAAGGAGAAT 
TTAGAGAGAA CTTTGTTCAG ACTTGAACAA GGTTTTGAGC TCCAGTTCCG CCTGGGTCCA 
AGCCTTCAAG GAAGGAGAGT CATAGTTCAC ACCAACTACC CACTTGATGG ACAGAAGTTC 
ATTCGAAACA ACTTCCGTGT TTTAGCCTGG AACTACCCAA CAGGAAGGGA GGATGACTCT 
GATAAGTACT GCTCCCTAGA GCTCAAGATT GCTGGATCCT ACCAGTACTA CTTTGGATAT 
GCAGGTGTGG AGAGGTTAGG AGGAGGCTAC ATCGTCGTTG ATCCTATTCT TCATGTTGGA 
GCAGACAACC ATGTCCTTCT GCTGGACTGT GTCACCATCC AGACGTACTT GTCCAAATGT 
CTGGGACGCC TTGATGACTG GCCAGACAGA CTGAGAGTAG CCAAGGAGTC AGGTTACAAC 
ATGATCCACT TCACTCCTCT GCAGACTTTG GGAGAGTCTA GGTCCTGTTA CTCCTTAGCT 
GACCAGCTGA CCTTTAGCCT GGAGTTCAGC CCAGATGGCA AGAGTTGCAC CTGGGAAGAT 
GTGGGAAGAC TGGTGGAAAA ACTGAGGATG GAATGGAATA TGGTATCAAT CACTGATGTG 
GTGTATAATC ACACTGCTGC AAACAGTGTG TGGATAAAGG AACATCCAGA GTGTGGCTAC 
AACCTGATGA ACTCTCCTCA TCTGCGTCCT GCCTGGGTCT TGGACAGAGC TATCTGGCAT 
TTAACCACTA GGGTAGCAGA TGGACGCTAC AAAGAGAAGG GACTTCCTTC TGACATTACC 
AACGAGTGCC ACCTAAATGC TCTCCGCAGG GTGTTTTGGG AGGACATGTT TCCTCAAATC 
AAACTTTGGG AGTTCTACCA GGTCAACGTG GAAAATGCTG TGGAGCAGTT CAGAATCCTG 
TTGCAAAATG GCACAAAGCC AGATCACAGC AAGACTGAAG GAAATAAAGG ATTAGAGATC 
ATTCAAGACC TCCAGTGCTG TCGCTATGGC AACAGAGTAA ACATGGACTC AGCTTTGGAG 
ACATTTGTAC CTCACAGCTC CTCTCCACAG CACATTGAAG AGTGCTGTGG TTGGCTGAAA 
CAGAAGTTGA CTGAGATGAA TGAAGAACAG TACCACATCA TACACCAGCA TCAGGAACAG 
GCAGTCAACT GCATTGTGGA GAACATAGTG TATGAACGTC TAGCTGACCA CGGTCCTAAA 
CTGGGTCCAG TTACCAGGAA AGAGCCTCTT GTCACCAGGT ATTTCACCTT CCCCTTTGAG 
GAAATGACTT TAGAGCAGGA GATGGAGTTT TTAAACCAAC CAGATAAGAT GTGCCACTTC 
TTGGCTCACA ACGGCTGGGT GATGGGTGAT GATCCATTGC GCAACTTTGC TGAACCAGGA 
TCCAATGTGT ACCTGCGTCG GGAGCTGATC TGCTGGGGGG ACAGTGTCAA ACTCCGCTAT 
GGGAAAGGAC CTGAGGATTG CCCCTACCTG TGGGCGCACA TGCAGAAATA CACTGAAATT 
ACTGCCAAAT ATTTCCACGG AGTCCGATTG GACAACTGCC ACTCTACGCC CTTGCATGTT 
GCTGAGTTCA TGTTGGATGT AGCCAGAAGC CAGTGTCCTA ACCTGTACGT GGTGGCTGAA 
CTGTTCACTG GCAGCGAGGA GCTGGATAAC ATCTTTGTTA CCAGACTAGG GATTACATCC 
CTCATACGAG AGGCCATGTC CGCCAGAGAC AGCCATGAAG AGGGCCGGCT GGTCTACCGT 
TACGGTGGAG AGCCGGTCGG AGCCTTTGTT CAGCCCAGCC TGCGTCCTCT CATGCCTTCT 
ATTGCTCACG CCATGTTCTT GGATGTGACC CATGACAACG AGTGTCCCAT TGAGCTGCGG 
TCAGCTCTCG ACTCGTTGCC CAGTTCTGCC ATCGTCTCCA TGGCCTGCTG TGCTACTGGC 
TCCACCAGAG GATATGATGA GCTGGTCCCA CATCAGATAT CTGTGGTGAA GGAGGAGCGT 
TTTTATCCCA AGTGGAATCC CTCTTCAGCT CCCACCTCTA CTGGTGAAGT TGGCTTTAAG 
AGCGGCATCA TCACTGGGAA GCTGGCTCTC AACAAGCTGC ACCAGGAGCT GGCTGCCCAG 
GGCTTTTCAC AGGTGTATGT GGACCAGGTC GATGCAGATA TTGTTGCAGT TACTCGTCAC 
TGCCCCAGCA CACACCAGTC TGTGGTAACA GTGAGCAGGA CAGCCTTCAA GAACCCCAAA 
ACTCATCAGT ACAGCAACAA TGTCCCACCT ATGTTCATAC CTGGCAAGAT TGAAGAAATT 
GTACTGGAGG CAAGGACAGT GGAGAGAGAG GCTAGGAGTT ACAAGAAGGA TGAGAAATAC 
ATAAACGGCA TGCCAGAATA CACAGTGGAA ATAAAAGAGC ATATACCGAT GGAGGAGAGT 
GCAGTGGTGA AACATGCTGG AGTGACATCT AAAGGCCGCT CAGAGTTTGT TCAAGAAATT 
CAGTTCCAGA AATTGACCCC TGGTAGCATC ATTGCCTTCA GAGTCAGTTT GGATCCCAAG 
GCACAGAAGC TGGTGGGCGT GTTGAGGTTT TATCTGTCCC AGTTCAGTCC AAAGTACAGG 
AGGGGCAGTG TACCTGATGA GAACCCACCA GAAGTGCTGC AGAAACCCCT TACGCAGTTA 
ATGTCTAAAC TGACGCTAGC CGACATGAAC ATCTTGCTGT TTCGCTGCGA AGCAGAAGAA 
CAAGAGGACG GTGGAGGCTG TTACAGTATC CCAGGCTGGG AGTGCCTGAA GTATGCAGGA 
CTACAAGGTC TGATGTCTGT ATTGGCGGAT ATCCGTCCCA CTAATGACCT TGGCCATCCC 
CTGTGTGCTA ACCTCAGAGA AGGAGACTGG CTCATAGACT TTGTCTCCAA TAGGCTGAAG 
CACAGAGATG GACCTCTGGC ACAGGTGGGT CAGTGGTTGG GTGCCATTTT TGACTACCTG 
AGGCACTTTC CTCGATATCT CATCCCCTGT TACTTTGACG CCATCTTGGT GTCAACCTAC 
ACAACAGCTC TGGATGCCAC GTTCAAACTC ATGTCGAGCT TCATTGAGCG AGGTTCCACG 
TTTGTCCGTC ACCTGGCTCT CGGCTCGGTT CAGATGTGTG GAGTTGGACG TTTTCCGGCT 
CTGCCTCCCA TTTCAACAAA AGTGCAGGAT GTCCCATGCC GCATCAGTCC TATCACTGGG 
GAAAAGGAGC AGTGTTGTGT CTCACTGGCA GCAGGTCTTC CTCATTTTTC TGCTGGGATT 
TTCCGTTGTT GGGGTAGAGA TACTTTCATC GCTCTCAGAG GACTTATGCT TATAACAGGG 
AGACACACTG AGGCACGCAA CATCATCCTG GCTTTTGCTG GAACCCTGCG TCACGGTTTA 
ATCCCCAACT TGCTGGGAGA AGGTCACAAT GCCAGATACA ACTGCAGGGA TGCTGTGTGG 
TGGTGGCTGC AGTGCATTCA GGACTATACC AATCTCGTTC CTGAGGGACA TGAAATTCTG 
CATTGCCCTG TCACCCGCAT GTACCCCACA GACGACTGTG AGCCTTGCAA ACCAGGGGAG 
GTTGAACAGC CATTGTATGA TGTCATCCAG GAGGCACTGC AGCGCCATCT GGAGGGTATT 
TCCTTCAGAG AGAGGAATGC TGGACCCAAG ATAGACAGGA ACATGAGAGA TGAAGGGTTC 
AATGTGGTTG CTAAAGTGGA CCCAGCAACA GGTTTTGTTA CCGGAGGAAA CCGCTTCAAC 
TGTGGCACTT GGATGGATAA AATGGGGGAG AGTGACAGAG CGAGGAACAA GGGGATGCCT 
GCAACTCCAA GAGACGGCGC GGCGGTGGAG ATCGTTGGTC TGAGTAAATC AGCAGTACGT 
TGGCTTGTGG AGCTGCAGTC TAAAGAACTG TTCCCCTATG ATGGAGTTAA GGTGCTTAGA 
GATGGAACAG ATGTGTTCAT CTTGTACACT GAGTGGAACA AGCAGCTTCA GCAGTCCTTT 
GAAGTTGAAT TCTGGGTGTC TGGGGACTCA CAGGACCCCA ATGAGAAGCA CCCTGATTTG 
GTGCATAAAA AGGGAATTTA TAAAGATAGT TATGGAGCTT CCAATCCCTG GTGTGACTAC 
CAGCTGAGGC CCAACTTCAC CATCGCAATG GTGGTGGCTC CAGAGCTGTT CACGTTAGAA 
AGAGCATGGA AGGCTTTGGA AATAGCTCAG AAGAAACTCT TGGGACCACT GGGAATGAAA 
ACTCTTGACC CAGATGACAT GGTCTACTGT GGTGTTTACA ATAATGCACT GGACAGCGAC 
AACTACAACC TGGCCAAGGG CTTTAACTAC CACCAAGGAC CTGAGTGGCT GTGGCCTGTT 
GGCTATTTTC TCCGTGCTAA GCTCTACTTT GCCAAGAAGT TAGGGGAGGA GGCCTACTCC 
AAGACCGTCA TTCTAGTGAA AAATGTTCTG TCACGACATT ATACCCACTT GGAGAGGTCT 
CTATGGAAAG GCTTACCAGA GCTGACCAAT GAGAATGGCC AGCATTGTCC CTTCAGCTGT 
GAGACCCAGG CCTGGTCTCT GGCTACTGTG CTGGAGGTGC TCCATGATCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023190607.1
CDS	125..4738
Translation	MMLPQHLKQIRVLMLNGKENLERTLFRLEQGFELQFRLGPSLQGRRVIVHTNYPLDGQKFIRNNFRVLAWNYPTGREDDSDKYCSLELKIAGSYQYYFGYAGVERLGGGYIVVDPILHVGADNHVLLLDCVTIQTYLSKCLGRLDDWPDRLRVAKESGYNMIHFTPLQTLGESRSCYSLADQLTFSLEFSPDGKSCTWEDVGRLVEKLRMEWNMVSITDVVYNHTAANSVWIKEHPECGYNLMNSPHLRPAWVLDRAIWHLTTRVADGRYKEKGLPSDITNECHLNALRRVFWEDMFPQIKLWEFYQVNVENAVEQFRILLQNGTKPDHSKTEGNKGLEIIQDLQCCRYGNRVNMDSALETFVPHSSSPQHIEECCGWLKQKLTEMNEEQYHIIHQHQEQAVNCIVENIVYERLADHGPKLGPVTRKEPLVTRYFTFPFEEMTLEQEMEFLNQPDKMCHFLAHNGWVMGDDPLRNFAEPGSNVYLRRELICWGDSVKLRYGKGPEDCPYLWAHMQKYTEITAKYFHGVRLDNCHSTPLHVAEFMLDVARSQCPNLYVVAELFTGSEELDNIFVTRLGITSLIREAMSARDSHEEGRLVYRYGGEPVGAFVQPSLRPLMPSIAHAMFLDVTHDNECPIELRSALDSLPSSAIVSMACCATGSTRGYDELVPHQISVVKEERFYPKWNPSSAPTSTGEVGFKSGIITGKLALNKLHQELAAQGFSQVYVDQVDADIVAVTRHCPSTHQSVVTVSRTAFKNPKTHQYSNNVPPMFIPGKIEEIVLEARTVEREARSYKKDEKYINGMPEYTVEIKEHIPMEESAVVKHAGVTSKGRSEFVQEIQFQKLTPGSIIAFRVSLDPKAQKLVGVLRFYLSQFSPKYRRGSVPDENPPEVLQKPLTQLMSKLTLADMNILLFRCEAEEQEDGGGCYSIPGWECLKYAGLQGLMSVLADIRPTNDLGHPLCANLREGDWLIDFVSNRLKHRDGPLAQVGQWLGAIFDYLRHFPRYLIPCYFDAILVSTYTTALDATFKLMSSFIERGSTFVRHLALGSVQMCGVGRFPALPPISTKVQDVPCRISPITGEKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLMLITGRHTEARNIILAFAGTLRHGLIPNLLGEGHNARYNCRDAVWWWLQCIQDYTNLVPEGHEILHCPVTRMYPTDDCEPCKPGEVEQPLYDVIQEALQRHLEGISFRERNAGPKIDRNMRDEGFNVVAKVDPATGFVTGGNRFNCGTWMDKMGESDRARNKGMPATPRDGAAVEIVGLSKSAVRWLVELQSKELFPYDGVKVLRDGTDVFILYTEWNKQLQQSFEVEFWVSGDSQDPNEKHPDLVHKKGIYKDSYGASNPWCDYQLRPNFTIAMVVAPELFTLERAWKALEIAQKKLLGPLGMKTLDPDDMVYCGVYNNALDSDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFAKKLGEEAYSKTVILVKNVLSRHYTHLERSLWKGLPELTNENGQHCPFSCETQAWSLATVLEVLHDL

Target ORF information:

RefSeq Version	XM_023334839.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023334839.1

ORF Insert Sequence:

ATGATGCTGC CTCAGCACCT CAAGCAAATC AGAGTCCTCA TGCTTAACGG AAAGGAGAAT 
TTAGAGAGAA CTTTGTTCAG ACTTGAACAA GGTTTTGAGC TCCAGTTCCG CCTGGGTCCA 
AGCCTTCAAG GAAGGAGAGT CATAGTTCAC ACCAACTACC CACTTGATGG ACAGAAGTTC 
ATTCGAAACA ACTTCCGTGT TTTAGCCTGG AACTACCCAA CAGGAAGGGA GGATGACTCT 
GATAAGTACT GCTCCCTAGA GCTCAAGATT GCTGGATCCT ACCAGTACTA CTTTGGATAT 
GCAGGTGTGG AGAGGTTAGG AGGAGGCTAC ATCGTCGTTG ATCCTATTCT TCATGTTGGA 
GCAGACAACC ATGTCCTTCT GCTGGACTGT GTCACCATCC AGACGTACTT GTCCAAATGT 
CTGGGACGCC TTGATGACTG GCCAGACAGA CTGAGAGTAG CCAAGGAGTC AGGTTACAAC 
ATGATCCACT TCACTCCTCT GCAGACTTTG GGAGAGTCTA GGTCCTGTTA CTCCTTAGCT 
GACCAGCTGA CCTTTAGCCT GGAGTTCAGC CCAGATGGCA AGAGTTGCAC CTGGGAAGAT 
GTGGGAAGAC TGGTGGAAAA ACTGAGGATG GAATGGAATA TGGTATCAAT CACTGATGTG 
GTGTATAATC ACACTGCTGC AAACAGTGTG TGGATAAAGG AACATCCAGA GTGTGGCTAC 
AACCTGATGA ACTCTCCTCA TCTGCGTCCT GCCTGGGTCT TGGACAGAGC TATCTGGCAT 
TTAACCACTA GGGTAGCAGA TGGACGCTAC AAAGAGAAGG GACTTCCTTC TGACATTACC 
AACGAGTGCC ACCTAAATGC TCTCCGCAGG GTGTTTTGGG AGGACATGTT TCCTCAAATC 
AAACTTTGGG AGTTCTACCA GGTCAACGTG GAAAATGCTG TGGAGCAGTT CAGAATCCTG 
TTGCAAAATG GCACAAAGCC AGATCACAGC AAGACTGAAG GAAATAAAGG ATTAGAGATC 
ATTCAAGACC TCCAGTGCTG TCGCTATGGC AACAGAGTAA ACATGGACTC AGCTTTGGAG 
ACATTTGTAC CTCACAGCTC CTCTCCACAG CACATTGAAG AGTGCTGTGG TTGGCTGAAA 
CAGAAGTTGA CTGAGATGAA TGAAGAACAG TACCACATCA TACACCAGCA TCAGGAACAG 
GCAGTCAACT GCATTGTGGA GAACATAGTG TATGAACGTC TAGCTGACCA CGGTCCTAAA 
CTGGGTCCAG TTACCAGGAA AGAGCCTCTT GTCACCAGGT ATTTCACCTT CCCCTTTGAG 
GAAATGACTT TAGAGCAGGA GATGGAGTTT TTAAACCAAC CAGATAAGAT GTGCCACTTC 
TTGGCTCACA ACGGCTGGGT GATGGGTGAT GATCCATTGC GCAACTTTGC TGAACCAGGA 
TCCAATGTGT ACCTGCGTCG GGAGCTGATC TGCTGGGGGG ACAGTGTCAA ACTCCGCTAT 
GGGAAAGGAC CTGAGGATTG CCCCTACCTG TGGGCGCACA TGCAGAAATA CACTGAAATT 
ACTGCCAAAT ATTTCCACGG AGTCCGATTG GACAACTGCC ACTCTACGCC CTTGCATGTT 
GCTGAGTTCA TGTTGGATGT AGCCAGAAGC CAGTGTCCTA ACCTGTACGT GGTGGCTGAA 
CTGTTCACTG GCAGCGAGGA GCTGGATAAC ATCTTTGTTA CCAGACTAGG GATTACATCC 
CTCATACGAG AGGCCATGTC CGCCAGAGAC AGCCATGAAG AGGGCCGGCT GGTCTACCGT 
TACGGTGGAG AGCCGGTCGG AGCCTTTGTT CAGCCCAGCC TGCGTCCTCT CATGCCTTCT 
ATTGCTCACG CCATGTTCTT GGATGTGACC CATGACAACG AGTGTCCCAT TGAGCTGCGG 
TCAGCTCTCG ACTCGTTGCC CAGTTCTGCC ATCGTCTCCA TGGCCTGCTG TGCTACTGGC 
TCCACCAGAG GATATGATGA GCTGGTCCCA CATCAGATAT CTGTGGTGAA GGAGGAGCGT 
TTTTATCCCA AGTGGAATCC CTCTTCAGCT CCCACCTCTA CTGGTGAAGT TGGCTTTAAG 
AGCGGCATCA TCACTGGGAA GCTGGCTCTC AACAAGCTGC ACCAGGAGCT GGCTGCCCAG 
GGCTTTTCAC AGGTGTATGT GGACCAGGTC GATGCAGATA TTGTTGCAGT TACTCGTCAC 
TGCCCCAGCA CACACCAGTC TGTGGTAACA GTGAGCAGGA CAGCCTTCAA GAACCCCAAA 
ACTCATCAGT ACAGCAACAA TGTCCCACCT ATGTTCATAC CTGGCAAGAT TGAAGAAATT 
GTACTGGAGG CAAGGACAGT GGAGAGAGAG GCTAGGAGTT ACAAGAAGGA TGAGAAATAC 
ATAAACGGCA TGCCAGAATA CACAGTGGAA ATAAAAGAGC ATATACCGAT GGAGGAGAGT 
GCAGTGGTGA AACATGCTGG AGTGACATCT AAAGGCCGCT CAGAGTTTGT TCAAGAAATT 
CAGTTCCAGA AATTGACCCC TGGTAGCATC ATTGCCTTCA GAGTCAGTTT GGATCCCAAG 
GCACAGAAGC TGGTGGGCGT GTTGAGGTTT TATCTGTCCC AGTTCAGTCC AAAGTACAGG 
AGGGGCAGTG TACCTGATGA GAACCCACCA GAAGTGCTGC AGAAACCCCT TACGCAGTTA 
ATGTCTAAAC TGACGCTAGC CGACATGAAC ATCTTGCTGT TTCGCTGCGA AGCAGAAGAA 
CAAGAGGACG GTGGAGGCTG TTACAGTATC CCAGGCTGGG AGTGCCTGAA GTATGCAGGA 
CTACAAGGTC TGATGTCTGT ATTGGCGGAT ATCCGTCCCA CTAATGACCT TGGCCATCCC 
CTGTGTGCTA ACCTCAGAGA AGGAGACTGG CTCATAGACT TTGTCTCCAA TAGGCTGAAG 
CACAGAGATG GACCTCTGGC ACAGGTGGGT CAGTGGTTGG GTGCCATTTT TGACTACCTG 
AGGCACTTTC CTCGATATCT CATCCCCTGT TACTTTGACG CCATCTTGGT GTCAACCTAC 
ACAACAGCTC TGGATGCCAC GTTCAAACTC ATGTCGAGCT TCATTGAGCG AGGTTCCACG 
TTTGTCCGTC ACCTGGCTCT CGGCTCGGTT CAGATGTGTG GAGTTGGACG TTTTCCGGCT 
CTGCCTCCCA TTTCAACAAA AGTGCAGGAT GTCCCATGCC GCATCAGTCC TATCACTGGG 
GAAAAGGAGC AGTGTTGTGT CTCACTGGCA GCAGGTCTTC CTCATTTTTC TGCTGGGATT 
TTCCGTTGTT GGGGTAGAGA TACTTTCATC GCTCTCAGAG GACTTATGCT TATAACAGGG 
AGACACACTG AGGCACGCAA CATCATCCTG GCTTTTGCTG GAACCCTGCG TCACGGTTTA 
ATCCCCAACT TGCTGGGAGA AGGTCACAAT GCCAGATACA ACTGCAGGGA TGCTGTGTGG 
TGGTGGCTGC AGTGCATTCA GGACTATACC AATCTCGTTC CTGAGGGACA TGAAATTCTG 
CATTGCCCTG TCACCCGCAT GTACCCCACA GACGACTGTG AGCCTTGCAA ACCAGGGGAG 
GTTGAACAGC CATTGTATGA TGTCATCCAG GAGGCACTGC AGCGCCATCT GGAGGGTATT 
TCCTTCAGAG AGAGGAATGC TGGACCCAAG ATAGACAGGA ACATGAGAGA TGAAGGGTTC 
AATGTGGTTG CTAAAGTGGA CCCAGCAACA GGTTTTGTTA CCGGAGGAAA CCGCTTCAAC 
TGTGGCACTT GGATGGATAA AATGGGGGAG AGTGACAGAG CGAGGAACAA GGGGATGCCT 
GCAACTCCAA GAGACGGCGC GGCGGTGGAG ATCGTTGGTC TGAGTAAATC AGCAGTACGT 
TGGCTTGTGG AGCTGCAGTC TAAAGAACTG TTCCCCTATG ATGGAGTTAA GGTGCTTAGA 
GATGGAACAG ATGTGTTCAT CTTGTACACT GAGTGGAACA AGCAGCTTCA GCAGTCCTTT 
GAAGTTGAAT TCTGGGTGTC TGGGGACTCA CAGGACCCCA ATGAGAAGCA CCCTGATTTG 
GTGCATAAAA AGGGAATTTA TAAAGATAGT TATGGAGCTT CCAATCCCTG GTGTGACTAC 
CAGCTGAGGC CCAACTTCAC CATCGCAATG GTGGTGGCTC CAGAGCTGTT CACGTTAGAA 
AGAGCATGGA AGGCTTTGGA AATAGCTCAG AAGAAACTCT TGGGACCACT GGGAATGAAA 
ACTCTTGACC CAGATGACAT GGTCTACTGT GGTGTTTACA ATAATGCACT GGACAGCGAC 
AACTACAACC TGGCCAAGGG CTTTAACTAC CACCAAGGAC CTGAGTGGCT GTGGCCTGTT 
GGCTATTTTC TCCGTGCTAA GCTCTACTTT GCCAAGAAGT TAGGGGAGGA GGCCTACTCC 
AAGACCGTCA TTCTAGTGAA AAATGTTCTG TCACGACATT ATACCCACTT GGAGAGGTCT 
CTATGGAAAG GCTTACCAGA GCTGACCAAT GAGAATGGCC AGCATTGTCC CTTCAGCTGT 
GAGACCCAGG CCTGGTCTCT GGCTACTGTG CTGGAGGTGC TCCATGATCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agl ( XM_023334841.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023190609.1 Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X4, mRNA.

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CloneID	OXi39000
Clone ID Related Accession (Same CDS sequence)	XM_023334841.1
Accession Version	XM_023334841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	glycogen debranching enzyme isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036448.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTCT CTTTGGAGAC CAGGGTTCCC TCCAAGTTCA GGAGAACTCC CATGATGCTG 
CCTCAGCACC TCAAGCAAAT CAGAGTCCTC ATGCTTAACG GAAAGGAGAA TTTAGAGAGA 
ACTTTGTTCA GACTTGAACA AGGTTTTGAG CTCCAGTTCC GCCTGGGTCC AAGCCTTCAA 
GGAAGGAGAG TCATAGTTCA CACCAACTAC CCACTTGATG GACAGAAGTT CATTCGAAAC 
AACTTCCGTG TTTTAGCCTG GAACTACCCA ACAGGAAGGG AGGATGACTC TGATAAGTAC 
TGCTCCCTAG AGCTCAAGAT TGCTGGATCC TACCAGTACT ACTTTGGATA TGCAGGTGTG 
GAGAGGTTAG GAGGAGGCTA CATCGTCGTT GATCCTATTC TTCATGTTGG AGCAGACAAC 
CATGTCCTTC TGCTGGACTG TGTCACCATC CAGACGTACT TGTCCAAATG TCTGGGACGC 
CTTGATGACT GGCCAGACAG ACTGAGAGTA GCCAAGGAGT CAGGTTACAA CATGATCCAC 
TTCACTCCTC TGCAGACTTT GGGAGAGTCT AGGTCCTGTT ACTCCTTAGC TGACCAGCTG 
ACCTTTAGCC TGGAGTTCAG CCCAGATGGC AAGAGTTGCA CCTGGGAAGA TGTGGGAAGA 
CTGGTGGAAA AACTGAGGAT GGAATGGAAT ATGGTATCAA TCACTGATGT GGTGTATAAT 
CACACTGCTG CAAACAGTGT GTGGATAAAG GAACATCCAG AGTGTGGCTA CAACCTGATG 
AACTCTCCTC ATCTGCGTCC TGCCTGGGTC TTGGACAGAG CTATCTGGCA TTTAACCACT 
AGGGTAGCAG ATGGACGCTA CAAAGAGAAG GGACTTCCTT CTGACATTAC CAACGAGTGC 
CACCTAAATG CTCTCCGCAG GGTGTTTTGG GAGGACATGT TTCCTCAAAT CAAACTTTGG 
GAGTTCTACC AGGTCAACGT GGAAAATGCT GTGGAGCAGT TCAGAATCCT GTTGCAAAAT 
GGCACAAAGC CAGATCACAG CAAGACTGAA GGAAATAAAG GATTAGAGAT CATTCAAGAC 
CTCCAGTGCT GTCGCTATGG CAACAGAGTA AACATGGACT CAGCTTTGGA GACATTTGTA 
CCTCACAGCT CCTCTCCACA GCACATTGAA GAGTGCTGTG GTTGGCTGAA ACAGAAGTTG 
ACTGAGATGA ATGAAGAACA GTACCACATC ATACACCAGC ATCAGGAACA GGCAGTCAAC 
TGCATTGTGG AGAACATAGT GTATGAACGT CTAGCTGACC ACGGTCCTAA ACTGGGTCCA 
GTTACCAGGA AAGAGCCTCT TGTCACCAGG TATTTCACCT TCCCCTTTGA GGAAATGACT 
TTAGAGCAGG AGATGGAGTT TTTAAACCAA CCAGATAAGA TGTGCCACTT CTTGGCTCAC 
AACGGCTGGG TGATGGGTGA TGATCCATTG CGCAACTTTG CTGAACCAGG ATCCAATGTG 
TACCTGCGTC GGGAGCTGAT CTGCTGGGGG GACAGTGTCA AACTCCGCTA TGGGAAAGGA 
CCTGAGGATT GCCCCTACCT GTGGGCGCAC ATGCAGAAAT ACACTGAAAT TACTGCCAAA 
TATTTCCACG GAGTCCGATT GGACAACTGC CACTCTACGC CCTTGCATGT TGCTGAGTTC 
ATGTTGGATG TAGCCAGAAG CCAGTGTCCT AACCTGTACG TGGTGGCTGA ACTGTTCACT 
GGCAGCGAGG AGCTGGATAA CATCTTTGTT ACCAGACTAG GGATTACATC CCTCATACGA 
GAGGCCATGT CCGCCAGAGA CAGCCATGAA GAGGGCCGGC TGGTCTACCG TTACGGTGGA 
GAGCCGGTCG GAGCCTTTGT TCAGCCCAGC CTGCGTCCTC TCATGCCTTC TATTGCTCAC 
GCCATGTTCT TGGATGTGAC CCATGACAAC GAGTGTCCCA TTGAGCTGCG GTCAGCTCTC 
GACTCGTTGC CCAGTTCTGC CATCGTCTCC ATGGCCTGCT GTGCTACTGG CTCCACCAGA 
GGATATGATG AGCTGGTCCC ACATCAGATA TCTGTGGTGA AGGAGGAGCG TTTTTATCCC 
AAGTGGAATC CCTCTTCAGC TCCCACCTCT ACTGGTGAAG TTGGCTTTAA GAGCGGCATC 
ATCACTGGGA AGCTGGCTCT CAACAAGCTG CACCAGGAGC TGGCTGCCCA GGGCTTTTCA 
CAGGTGTATG TGGACCAGGT CGATGCAGAT ATTGTTGCAG TTACTCGTCA CTGCCCCAGC 
ACACACCAGT CTGTGGTAAC AGTGAGCAGG ACAGCCTTCA AGAACCCCAA AACTCATCAG 
TACAGCAACA ATGTCCCACC TATGTTCATA CCTGGCAAGA TTGAAGAAAT TGTACTGGAG 
GCAAGGACAG TGGAGAGAGA GGCTAGGAGT TACAAGAAGG ATGAGAAATA CATAAACGGC 
ATGCCAGAAT ACACAGTGGA AATAAAAGAG CATATACCGA TGGAGGAGAG TGCAGTGGTG 
AAACATGCTG GAGTGACATC TAAAGGCCGC TCAGAGTTTG TTCAAGAAAT TCAGTTCCAG 
AAATTGACCC CTGGTAGCAT CATTGCCTTC AGAGTCAGTT TGGATCCCAA GGCACAGAAG 
CTGGTGGGCG TGTTGAGGTT TTATCTGTCC CAGTTCAGTC CAAAGTACAG GAGGGGCAGT 
GTACCTGATG AGAACCCACC AGAAGTGCTG CAGAAACCCC TTACGCAGTT AATGTCTAAA 
CTGACGCTAG CCGACATGAA CATCTTGCTG TTTCGCTGCG AAGCAGAAGA ACAAGAGGAC 
GGTGGAGGCT GTTACAGTAT CCCAGGCTGG GAGTGCCTGA AGTATGCAGG ACTACAAGGT 
CTGATGTCTG TATTGGCGGA TATCCGTCCC ACTAATGACC TTGGCCATCC CCTGTGTGCT 
AACCTCAGAG AAGGAGACTG GCTCATAGAC TTTGTCTCCA ATAGGCTGAA GCACAGAGAT 
GGACCTCTGG CACAGGTGGG TCAGTGGTTG GGTGCCATTT TTGACTACCT GAGGCACTTT 
CCTCGATATC TCATCCCCTG TTACTTTGAC GCCATCTTGG TGTCAACCTA CACAACAGCT 
CTGGATGCCA CGTTCAAACT CATGTCGAGC TTCATTGAGC GAGGTTCCAC GTTTGTCCGT 
CACCTGGCTC TCGGCTCGGT TCAGATGTGT GGAGTTGGAC GTTTTCCGGC TCTGCCTCCC 
ATTTCAACAA AAGTGCAGGA TGTCCCATGC CGCATCAGTC CTATCACTGG GGAAAAGGAG 
CAGTGTTGTG TCTCACTGGC AGCAGGTCTT CCTCATTTTT CTGCTGGGAT TTTCCGTTGT 
TGGGGTAGAG ATACTTTCAT CGCTCTCAGA GGACTTATGC TTATAACAGG GAGACACACT 
GAGGCACGCA ACATCATCCT GGCTTTTGCT GGAACCCTGC GTCACGGTTT AATCCCCAAC 
TTGCTGGGAG AAGGTCACAA TGCCAGATAC AACTGCAGGG ATGCTGTGTG GTGGTGGCTG 
CAGTGCATTC AGGACTATAC CAATCTCGTT CCTGAGGGAC ATGAAATTCT GCATTGCCCT 
GTCACCCGCA TGTACCCCAC AGACGACTGT GAGCCTTGCA AACCAGGGGA GGTTGAACAG 
CCATTGTATG ATGTCATCCA GGAGGCACTG CAGCGCCATC TGGAGGGTAT TTCCTTCAGA 
GAGAGGAATG CTGGACCCAA GATAGACAGG AACATGAGAG ATGAAGGGTT CAATGTGGTT 
GCTAAAGTGG ACCCAGCAAC AGGTTTTGTT ACCGGAGGAA ACCGCTTCAA CTGTGGCACT 
TGGATGGATA AAATGGGGGA GAGTGACAGA GCGAGGAACA AGGGGATGCC TGCAACTCCA 
AGAGACGGCG CGGCGGTGGA GATCGTTGGT CTGAGTAAAT CAGCAGTACG TTGGCTTGTG 
GAGCTGCAGT CTAAAGAACT GTTCCCCTAT GATGGAGTTA AGGTGCTTAG AGATGGAACA 
GATGTGTTCA TCTTGTACAC TGAGTGGAAC AAGCAGCTTC AGCAGTCCTT TGAAGTTGAA 
TTCTGGGTGT CTGGGGACTC ACAGGACCCC AATGAGAAGC ACCCTGATTT GGTGCATAAA 
AAGGGAATTT ATAAAGATAG TTATGGAGCT TCCAATCCCT GGTGTGACTA CCAGCTGAGG 
CCCAACTTCA CCATCGCAAT GGTGGTGGCT CCAGAGCTGT TCACGTTAGA AAGAGCATGG 
AAGGCTTTGG AAATAGCTCA GAAGAAACTC TTGGGACCAC TGGGAATGAA AACTCTTGAC 
CCAGATGACA TGGTCTACTG TGGTGTTTAC AATAATGCAC TGGACAGCGA CAACTACAAC 
CTGGCCAAGG GCTTTAACTA CCACCAAGGA CCTGAGTGGC TGTGGCCTGT TGGCTATTTT 
CTCCGTGCTA AGCTCTACTT TGCCAAGAAG TTAGGGGAGG AGGCCTACTC CAAGACCGTC 
ATTCTAGTGA AAAATGTTCT GTCACGACAT TATACCCACT TGGAGAGGTC TCTATGGAAA 
GGCTTACCAG AGCTGACCAA TGAGAATGGC CAGCATTGTC CCTTCAGCTG TGAGACCCAG 
GCCTGGTCTC TGGCTACTGT GCTGGAGGTG CTCCATGATC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023190609.1
CDS	146..4810
Translation	MPVSLETRVPSKFRRTPMMLPQHLKQIRVLMLNGKENLERTLFRLEQGFELQFRLGPSLQGRRVIVHTNYPLDGQKFIRNNFRVLAWNYPTGREDDSDKYCSLELKIAGSYQYYFGYAGVERLGGGYIVVDPILHVGADNHVLLLDCVTIQTYLSKCLGRLDDWPDRLRVAKESGYNMIHFTPLQTLGESRSCYSLADQLTFSLEFSPDGKSCTWEDVGRLVEKLRMEWNMVSITDVVYNHTAANSVWIKEHPECGYNLMNSPHLRPAWVLDRAIWHLTTRVADGRYKEKGLPSDITNECHLNALRRVFWEDMFPQIKLWEFYQVNVENAVEQFRILLQNGTKPDHSKTEGNKGLEIIQDLQCCRYGNRVNMDSALETFVPHSSSPQHIEECCGWLKQKLTEMNEEQYHIIHQHQEQAVNCIVENIVYERLADHGPKLGPVTRKEPLVTRYFTFPFEEMTLEQEMEFLNQPDKMCHFLAHNGWVMGDDPLRNFAEPGSNVYLRRELICWGDSVKLRYGKGPEDCPYLWAHMQKYTEITAKYFHGVRLDNCHSTPLHVAEFMLDVARSQCPNLYVVAELFTGSEELDNIFVTRLGITSLIREAMSARDSHEEGRLVYRYGGEPVGAFVQPSLRPLMPSIAHAMFLDVTHDNECPIELRSALDSLPSSAIVSMACCATGSTRGYDELVPHQISVVKEERFYPKWNPSSAPTSTGEVGFKSGIITGKLALNKLHQELAAQGFSQVYVDQVDADIVAVTRHCPSTHQSVVTVSRTAFKNPKTHQYSNNVPPMFIPGKIEEIVLEARTVEREARSYKKDEKYINGMPEYTVEIKEHIPMEESAVVKHAGVTSKGRSEFVQEIQFQKLTPGSIIAFRVSLDPKAQKLVGVLRFYLSQFSPKYRRGSVPDENPPEVLQKPLTQLMSKLTLADMNILLFRCEAEEQEDGGGCYSIPGWECLKYAGLQGLMSVLADIRPTNDLGHPLCANLREGDWLIDFVSNRLKHRDGPLAQVGQWLGAIFDYLRHFPRYLIPCYFDAILVSTYTTALDATFKLMSSFIERGSTFVRHLALGSVQMCGVGRFPALPPISTKVQDVPCRISPITGEKEQCCVSLAAGLPHFSAGIFRCWGRDTFIALRGLMLITGRHTEARNIILAFAGTLRHGLIPNLLGEGHNARYNCRDAVWWWLQCIQDYTNLVPEGHEILHCPVTRMYPTDDCEPCKPGEVEQPLYDVIQEALQRHLEGISFRERNAGPKIDRNMRDEGFNVVAKVDPATGFVTGGNRFNCGTWMDKMGESDRARNKGMPATPRDGAAVEIVGLSKSAVRWLVELQSKELFPYDGVKVLRDGTDVFILYTEWNKQLQQSFEVEFWVSGDSQDPNEKHPDLVHKKGIYKDSYGASNPWCDYQLRPNFTIAMVVAPELFTLERAWKALEIAQKKLLGPLGMKTLDPDDMVYCGVYNNALDSDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFAKKLGEEAYSKTVILVKNVLSRHYTHLERSLWKGLPELTNENGQHCPFSCETQAWSLATVLEVLHDL

Target ORF information:

RefSeq Version	XM_023334841.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (agl), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023334841.1

ORF Insert Sequence:

ATGCCTGTCT CTTTGGAGAC CAGGGTTCCC TCCAAGTTCA GGAGAACTCC CATGATGCTG 
CCTCAGCACC TCAAGCAAAT CAGAGTCCTC ATGCTTAACG GAAAGGAGAA TTTAGAGAGA 
ACTTTGTTCA GACTTGAACA AGGTTTTGAG CTCCAGTTCC GCCTGGGTCC AAGCCTTCAA 
GGAAGGAGAG TCATAGTTCA CACCAACTAC CCACTTGATG GACAGAAGTT CATTCGAAAC 
AACTTCCGTG TTTTAGCCTG GAACTACCCA ACAGGAAGGG AGGATGACTC TGATAAGTAC 
TGCTCCCTAG AGCTCAAGAT TGCTGGATCC TACCAGTACT ACTTTGGATA TGCAGGTGTG 
GAGAGGTTAG GAGGAGGCTA CATCGTCGTT GATCCTATTC TTCATGTTGG AGCAGACAAC 
CATGTCCTTC TGCTGGACTG TGTCACCATC CAGACGTACT TGTCCAAATG TCTGGGACGC 
CTTGATGACT GGCCAGACAG ACTGAGAGTA GCCAAGGAGT CAGGTTACAA CATGATCCAC 
TTCACTCCTC TGCAGACTTT GGGAGAGTCT AGGTCCTGTT ACTCCTTAGC TGACCAGCTG 
ACCTTTAGCC TGGAGTTCAG CCCAGATGGC AAGAGTTGCA CCTGGGAAGA TGTGGGAAGA 
CTGGTGGAAA AACTGAGGAT GGAATGGAAT ATGGTATCAA TCACTGATGT GGTGTATAAT 
CACACTGCTG CAAACAGTGT GTGGATAAAG GAACATCCAG AGTGTGGCTA CAACCTGATG 
AACTCTCCTC ATCTGCGTCC TGCCTGGGTC TTGGACAGAG CTATCTGGCA TTTAACCACT 
AGGGTAGCAG ATGGACGCTA CAAAGAGAAG GGACTTCCTT CTGACATTAC CAACGAGTGC 
CACCTAAATG CTCTCCGCAG GGTGTTTTGG GAGGACATGT TTCCTCAAAT CAAACTTTGG 
GAGTTCTACC AGGTCAACGT GGAAAATGCT GTGGAGCAGT TCAGAATCCT GTTGCAAAAT 
GGCACAAAGC CAGATCACAG CAAGACTGAA GGAAATAAAG GATTAGAGAT CATTCAAGAC 
CTCCAGTGCT GTCGCTATGG CAACAGAGTA AACATGGACT CAGCTTTGGA GACATTTGTA 
CCTCACAGCT CCTCTCCACA GCACATTGAA GAGTGCTGTG GTTGGCTGAA ACAGAAGTTG 
ACTGAGATGA ATGAAGAACA GTACCACATC ATACACCAGC ATCAGGAACA GGCAGTCAAC 
TGCATTGTGG AGAACATAGT GTATGAACGT CTAGCTGACC ACGGTCCTAA ACTGGGTCCA 
GTTACCAGGA AAGAGCCTCT TGTCACCAGG TATTTCACCT TCCCCTTTGA GGAAATGACT 
TTAGAGCAGG AGATGGAGTT TTTAAACCAA CCAGATAAGA TGTGCCACTT CTTGGCTCAC 
AACGGCTGGG TGATGGGTGA TGATCCATTG CGCAACTTTG CTGAACCAGG ATCCAATGTG 
TACCTGCGTC GGGAGCTGAT CTGCTGGGGG GACAGTGTCA AACTCCGCTA TGGGAAAGGA 
CCTGAGGATT GCCCCTACCT GTGGGCGCAC ATGCAGAAAT ACACTGAAAT TACTGCCAAA 
TATTTCCACG GAGTCCGATT GGACAACTGC CACTCTACGC CCTTGCATGT TGCTGAGTTC 
ATGTTGGATG TAGCCAGAAG CCAGTGTCCT AACCTGTACG TGGTGGCTGA ACTGTTCACT 
GGCAGCGAGG AGCTGGATAA CATCTTTGTT ACCAGACTAG GGATTACATC CCTCATACGA 
GAGGCCATGT CCGCCAGAGA CAGCCATGAA GAGGGCCGGC TGGTCTACCG TTACGGTGGA 
GAGCCGGTCG GAGCCTTTGT TCAGCCCAGC CTGCGTCCTC TCATGCCTTC TATTGCTCAC 
GCCATGTTCT TGGATGTGAC CCATGACAAC GAGTGTCCCA TTGAGCTGCG GTCAGCTCTC 
GACTCGTTGC CCAGTTCTGC CATCGTCTCC ATGGCCTGCT GTGCTACTGG CTCCACCAGA 
GGATATGATG AGCTGGTCCC ACATCAGATA TCTGTGGTGA AGGAGGAGCG TTTTTATCCC 
AAGTGGAATC CCTCTTCAGC TCCCACCTCT ACTGGTGAAG TTGGCTTTAA GAGCGGCATC 
ATCACTGGGA AGCTGGCTCT CAACAAGCTG CACCAGGAGC TGGCTGCCCA GGGCTTTTCA 
CAGGTGTATG TGGACCAGGT CGATGCAGAT ATTGTTGCAG TTACTCGTCA CTGCCCCAGC 
ACACACCAGT CTGTGGTAAC AGTGAGCAGG ACAGCCTTCA AGAACCCCAA AACTCATCAG 
TACAGCAACA ATGTCCCACC TATGTTCATA CCTGGCAAGA TTGAAGAAAT TGTACTGGAG 
GCAAGGACAG TGGAGAGAGA GGCTAGGAGT TACAAGAAGG ATGAGAAATA CATAAACGGC 
ATGCCAGAAT ACACAGTGGA AATAAAAGAG CATATACCGA TGGAGGAGAG TGCAGTGGTG 
AAACATGCTG GAGTGACATC TAAAGGCCGC TCAGAGTTTG TTCAAGAAAT TCAGTTCCAG 
AAATTGACCC CTGGTAGCAT CATTGCCTTC AGAGTCAGTT TGGATCCCAA GGCACAGAAG 
CTGGTGGGCG TGTTGAGGTT TTATCTGTCC CAGTTCAGTC CAAAGTACAG GAGGGGCAGT 
GTACCTGATG AGAACCCACC AGAAGTGCTG CAGAAACCCC TTACGCAGTT AATGTCTAAA 
CTGACGCTAG CCGACATGAA CATCTTGCTG TTTCGCTGCG AAGCAGAAGA ACAAGAGGAC 
GGTGGAGGCT GTTACAGTAT CCCAGGCTGG GAGTGCCTGA AGTATGCAGG ACTACAAGGT 
CTGATGTCTG TATTGGCGGA TATCCGTCCC ACTAATGACC TTGGCCATCC CCTGTGTGCT 
AACCTCAGAG AAGGAGACTG GCTCATAGAC TTTGTCTCCA ATAGGCTGAA GCACAGAGAT 
GGACCTCTGG CACAGGTGGG TCAGTGGTTG GGTGCCATTT TTGACTACCT GAGGCACTTT 
CCTCGATATC TCATCCCCTG TTACTTTGAC GCCATCTTGG TGTCAACCTA CACAACAGCT 
CTGGATGCCA CGTTCAAACT CATGTCGAGC TTCATTGAGC GAGGTTCCAC GTTTGTCCGT 
CACCTGGCTC TCGGCTCGGT TCAGATGTGT GGAGTTGGAC GTTTTCCGGC TCTGCCTCCC 
ATTTCAACAA AAGTGCAGGA TGTCCCATGC CGCATCAGTC CTATCACTGG GGAAAAGGAG 
CAGTGTTGTG TCTCACTGGC AGCAGGTCTT CCTCATTTTT CTGCTGGGAT TTTCCGTTGT 
TGGGGTAGAG ATACTTTCAT CGCTCTCAGA GGACTTATGC TTATAACAGG GAGACACACT 
GAGGCACGCA ACATCATCCT GGCTTTTGCT GGAACCCTGC GTCACGGTTT AATCCCCAAC 
TTGCTGGGAG AAGGTCACAA TGCCAGATAC AACTGCAGGG ATGCTGTGTG GTGGTGGCTG 
CAGTGCATTC AGGACTATAC CAATCTCGTT CCTGAGGGAC ATGAAATTCT GCATTGCCCT 
GTCACCCGCA TGTACCCCAC AGACGACTGT GAGCCTTGCA AACCAGGGGA GGTTGAACAG 
CCATTGTATG ATGTCATCCA GGAGGCACTG CAGCGCCATC TGGAGGGTAT TTCCTTCAGA 
GAGAGGAATG CTGGACCCAA GATAGACAGG AACATGAGAG ATGAAGGGTT CAATGTGGTT 
GCTAAAGTGG ACCCAGCAAC AGGTTTTGTT ACCGGAGGAA ACCGCTTCAA CTGTGGCACT 
TGGATGGATA AAATGGGGGA GAGTGACAGA GCGAGGAACA AGGGGATGCC TGCAACTCCA 
AGAGACGGCG CGGCGGTGGA GATCGTTGGT CTGAGTAAAT CAGCAGTACG TTGGCTTGTG 
GAGCTGCAGT CTAAAGAACT GTTCCCCTAT GATGGAGTTA AGGTGCTTAG AGATGGAACA 
GATGTGTTCA TCTTGTACAC TGAGTGGAAC AAGCAGCTTC AGCAGTCCTT TGAAGTTGAA 
TTCTGGGTGT CTGGGGACTC ACAGGACCCC AATGAGAAGC ACCCTGATTT GGTGCATAAA 
AAGGGAATTT ATAAAGATAG TTATGGAGCT TCCAATCCCT GGTGTGACTA CCAGCTGAGG 
CCCAACTTCA CCATCGCAAT GGTGGTGGCT CCAGAGCTGT TCACGTTAGA AAGAGCATGG 
AAGGCTTTGG AAATAGCTCA GAAGAAACTC TTGGGACCAC TGGGAATGAA AACTCTTGAC 
CCAGATGACA TGGTCTACTG TGGTGTTTAC AATAATGCAC TGGACAGCGA CAACTACAAC 
CTGGCCAAGG GCTTTAACTA CCACCAAGGA CCTGAGTGGC TGTGGCCTGT TGGCTATTTT 
CTCCGTGCTA AGCTCTACTT TGCCAAGAAG TTAGGGGAGG AGGCCTACTC CAAGACCGTC 
ATTCTAGTGA AAAATGTTCT GTCACGACAT TATACCCACT TGGAGAGGTC TCTATGGAAA 
GGCTTACCAG AGCTGACCAA TGAGAATGGC CAGCATTGTC CCTTCAGCTG TGAGACCCAG 
GCCTGGTCTC TGGCTACTGT GCTGGAGGTG CTCCATGATC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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agl cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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