DOPEY1 cDNA ORF clone, Xiphophorus maculatus(Southern platyfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	DOPEY1
Entrez Gene ID	102218900
Full Name	dopey family member 1
Gene Type	protein-coding
Organism	Xiphophorus maculatus(Southern platyfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023330621.1	XP_023186389.1	protein dopey-1 isoform X1
XM_023330623.1	XP_023186391.1	protein dopey-1 isoform X2
XM_023330624.1	XP_023186392.1	protein dopey-1 isoform X3
XM_023330625.1	XP_023186393.1	protein dopey-1 isoform X4
XM_023330626.1	XP_023186394.1	protein dopey-1 isoform X5
XM_023330627.1	XP_023186395.1	protein dopey-1 isoform X6
XM_023330622.1	XP_023186390.1	protein dopey-1 isoform X1
XM_014472742.1	XP_014328228.1	LOW QUALITY PROTEIN: protein dopey-1

Pathways from BioSystems
Gene Ontology

The following DOPEY1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DOPEY1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXi14919	XM_014472742.1 Latest version!	Xiphophorus maculatus dopey family member 1 (dopey1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXi35839	XM_023330621.1 Latest version!	Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXi35839	XM_023330622.1 Latest version!	Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXi35840	XM_023330623.1 Latest version!	Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXi35841	XM_023330624.1 Latest version!	Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXi35842	XM_023330625.1 Latest version!	Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXi35843	XM_023330626.1 Latest version!	Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXi35844	XM_023330627.1 Latest version!	Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DOPEY1 ( XM_014472742.1 ) cDNA ORF clone, Xiphophorus maculatus(Southern platyfish) -> XP_014328228.1 Xiphophorus maculatus dopey family member 1 (dopey1), mRNA.

Price & Availability↑

CloneID	OXi14919
Clone ID Related Accession (Same CDS sequence)	XM_014472742.1
Accession Version	XM_014472742.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1445702400000
Organism	Xiphophorus maculatus(Southern platyfish)
Product	LOW QUALITY PROTEIN: protein dopey-1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005372453.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xiphophorus maculatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## internal stop codons :: corrected 2 genomic stop codon ##RefSeq-Attributes-END##

ATGAATGCAG AGGAAGTGGA GCTGCTCAGC GACTCAAAGT ACAGGAACTA TGTGGCAGCA 
GTGGACAAAG CCCTCAAGAA CTTTGAGTAT TCCAGCGAGT GGGCCGACCT CATCTCCGCA 
CTGGGCAAGC TCAACAAGGT TTTGCAGAAC AATGCAAAAT ATCAAGTTGT CCCCAAGAAG 
CTGACAATAG GCAAACGGCT GGCCCAGTGC CTCCACCCAG CCCTGCCCAG CGGCGTGCAC 
CGGAAGGCCT TGGAGACCTA CGAGATCATT TTCAAGATCA TTGGGCCCAA GAGGCTGGCC 
AAAGACCTCT TTCTGTACAG CTCGGGACTC TTCCCACTAC TCTCCAACGC TGCTATGTCT 
GTGAAGCCCG TGTTGCTGGG GCTCTATGAG ACCTACTACC TGCCCCTGGG GAAGACTCTC 
AAACCAGGCC TGCAGGGCTT ACTGACCGGC GTGCTGCCTG GTCTGGAGGA AGGTTCAGAG 
TATTACGACA GGTAGGAAAA GAGAAGTGCT GCTGCAACAA CATGAGCGCA CACCAGTGCC 
TTGTGGGGCA GCATCCTGAC CAGCCCCTCT GTGCGTCTCC CCGGGGTCTC CTTCGTTCTG 
CTGCACCTCA ACCGGAAGCT CTCCATGGAG GACCAGCTCT ACGTCATGGG CAGCGACATT 
GAGCTTATGG TGGAGGCTGT TAGTACCTCG GTGCAGGACT CAAGCGTTTT GGTCCAGAGA 
AGCACTCTGG ACCTCATCCT CTTCTGCTTC CCCTTCCATA TGAGCCAGGC TACCCGCCCT 
GACATGATCC GGATCCTTTC AGCAGCTTTG CATGTGGTTC TGAGAAGAGA CATGTCTCTG 
AACCGCAGAC TCTACGCCTG GCTGCTGGGC TTTGACAATA ATGGCGGGAT GTCAGGCCCC 
CGCAGTACTC GGCAGAGCAA CCCGGAGGAA CACGCTTCCC ACTACTTCAA CAGCTTCTCC 
AAGGACATGC TCGTCCAGGC AATGGTTGGG ATTCTGCAGG GCAAGGCTCG AGGTGGGGAG 
GAGGAGAGCA TCCTGATGCA CGACCTCAAG CCATTTCGCA TCCTGATCAG TCTGCTGGAC 
AAACCAGAAC TAGGGCCGGC CATTCTAGAG GACGTTCTGA TCGAAGTGTT TCGGACTCTT 
TACACACAGT GCCGGACAGA GTTGGACCTT CAAAACCAGA GTCCGTTCAG CAAAGATCAC 
ACACACCTCA GCAGTAAACT GCGAGAAAAC AAGAAGACGG CAGAGCTTAT CAAAACAGCC 
AATCTCCTGT TCAACTCGTT TGAGCCGTAC TACATGTGGG ACTACATCGC TCGCTGGTTT 
GAGGAGTGCT GCAGGAGAAC ATTGAACCGC AGCACACGTG TTGCACGATA CGGTTGGAGT 
CTGGATTCTC CTGATCTTTC TCTGGTGGAG TTCTGTCAGT TGGTAGATTT TCTACTAGAC 
ATTGTTTCAT TGCCTACTAG AAGCATGAGG GTAATCTGCC AGGAGACGTA CATAGAAATC 
CAGACGGAGC ATCTCCCTCA ACTGCTGTTG CGGATGGTGG CAGCTCTGAC CTGTCATCTC 
CAGGCGCTGG ACCTCGGGGA GCTCACCCAC TGCCTTCGCC TCTGCTCCAA GATCCTCAGC 
AAGGTGCAGC CTCCGTTGGT GTCCCCGCTC GCCCTCCCCG TGAGTCCACA GCCGCCGGGC 
CTGGCGGCAC AGAACAAAAG CAGAGATGTG GATGTTAAAC AGACTCCTCC CTCTGTCCCA 
GAGATGACCG ACAGTGGGGA CGTGTTTGAC GATGGGGAAA TCCCTACTGG CAGTCGCTCT 
GCAGAAAGTG GCTTCACAGA CTTTGTTCAA TACCGAGAAG ATGGTTCCGA GGATCCAGAG 
CGAGCCTCTC ATCCCCACCC GCCTCTTAGA GCAGGCAGTC GTCCGTCAGG CTCGTGTCAG 
TCCAAGCCTT TGGACAAACC AGTCATGCAG TGTTGCCTGG AGCACTTCCA GCAGTTTCTC 
TCCTGTCTGA TCACTTTTTA TATTGTTCCT GAGCACCTGG ACAAAGCTGA GGGTCAGAGA 
GGTGAGCTCT TGCATTCGGG GCCTTTGGTT TCCGCAGGTT CCCAGCCCAG TAATTATACG 
GAGCCGTGCT CAGGAGCTGG AGTGGTCCAG AAAGATTGTC TGGCTGCATT TACTGCTGCC 
TGTCAGCTCT TCTTAGAATG CTCCAGTTTC CCAGTCTACA TTGCAGAGGG CAACCTCAAG 
TCCTCGCCTG CACAGGAAGA GCAATGCGAC AGTGAGCAGG TTCGGCTGCC AGTGTGGCTG 
CAGACCCTGA TGGATGCCTG CTGCCTGGCT GGTGACTTCA GCCTACAGGG TGTTGCCATC 
TCCCTGCTTA TGGACCTAAT AGGCCTGACT CAGTCAGTTG CTGTGGTGAC GGCAGAGAGT 
CTGGCTTCCA GTAGCAGTAC GGAGCCCACT CAGCCAATGA GCCCCAGCCA GGGTCGGGTG 
GCTGTGGTCA TTAGGCCTCC ACTCACTCAG GGAATCCTGA AGTATATTGC GGATAAAACG 
GACTTCTTCA AGAGCGTTGC AGTGATATTA TGGGACCAGC TGGGTGAAGG AACTCCTCAG 
CATCACCAGC GCAGTGTTGA GCTCTTTTAT CAGCTACACA ACCTGGTCCC TTCATCCAGC 
ACCTGTGAGG ACGTCATAAG CCAGCAACTC ATGCACAGAG ACAAGAGAAT TCGTTTGGCA 
GCCCACGTGA AGTTCTCTGT TCTGTGGCAT TTAACGCGAG ACTTGAACAT CACCAAGTCT 
TCTCCGTTTA GTCGCACATT TGACAGGTCT CTCTTCATCA TGCTCGACAG CTTAAGTTAT 
TGGGATCCAT GTACCAGTGC GGTCGGTCGG GCTTGGTTAA ATCAAGTCCT TCAGAGACAT 
GACATTGCAC GGGTTCTTGA GCCACTCCTA CTTCTTCTGC TCCATCCTAA AACTCACAGG 
GTTTCTATTC AGAAGGTGCA AGCCCAACGC CATTGGGCGC AGATATTTCC TGATCCACCT 
GAGCAGGAAC CAACAGAACC AATCTACATT AGAGACTCCG GCTTCTCAGA CAACTTTCTT 
CAGATTCGAA GCGAAGGGGG TGGGCATGAG GACTTCAGAG GTCTGACAAT AGGTGACATG 
GAGCCATTTT GCCTGACGGT AAACCCCCTA AGTGACAGCT TGTCCATTCT TAGCCTGAGC 
AGTGAAAACT TGCACTTAGC TGGTGAGTAT CAGACCAGGG ACCAGCAGGA GGAGCAACAG 
AGTTCAAAAT CAAGTGGTTC TCATTCATCT ACACTGGAAA ACGGTAGCTT TGAAGACCCT 
GAGGGGGTCA GCTACATCGG AAATGGCTCA GATCGACCGC TTGGTTCATC TGATGAAACG 
CCTGACGACT CCATAGAGGA AGCTGTGTCT TGTGTAGTGA AGGACCTGAT TGATAGGGTT 
CTGAATTTAG TTGATGAGGA ATCAAATGAA ACCTCATCTC CACCTGAAAA TTGGCCCCAA 
ACTGATACTG AAAGCACTTC TTCAGACACC TCCACCGGGA TCCGCCTGGA CTCCTGTCCT 
CCTCAATGCT CCAACCACCA GAATCTGCCA CAGTTATTGG CTGAAGGCAC ACTAGAGTTC 
CTCTCTGTTA CTCCAGCGGA GGGGCTACAC AAAGAGGGCA TCGCCCGCCA CAGTTCATCG 
CCCTCCATCG TCATGTTGCC AGACAGCACT GATGCAGCCA CTCCAGACCG CAGCCTGCAG 
GTGGAGGACC CTCAGGCCCG CAAACGTAGC CACAGCAGCA CTCAGCTTAG CCTTAAAGGG 
AAGATCATGG AGAAGCTGGT AGACAAGTCT CCAGGGGCAA AGCCCAAGAT CAAGAAGACT 
AAGAGGAAAG AGGAAGAGAA GCAGAAAAAA ACGAACCAAG CTGAGAAACT GCATCCACTC 
AGTATTTTCT TTGGAGACAG TCTAGACCTC GAGAACTGGT ACAGCTGTGG TGAAGGGGAG 
GTGTCGGAGA TCGAGAGTGA CATGGGCTCA CCGAGCGGGG GCTCTTGCGG GACCGGTGCT 
GGGGCGAACG TCACAGGGCG CAGATCGTCC TCCACTCTGC CTCGTTTTAA CATCCATCCT 
CTCTACCAGC ACGTCCTGCT CTACCTACAG CTCTACGACT CCTCTAGGAC CCTGCATGCC 
CTGTCGGCGA TCGCAGCCAT GGTGAGAGCC GCTCCCTCAG GCTTTGTAAG CGCCATCTCC 
ACCACCAGCA TCAATAACAC CTACACTCCA CAGCTCTCCT TGCTGCAAAA CCTACTTGCT 
CGCCACAGAG TCTCTGTTAT GGGCAAAGAC TTCTACTGCC CCATTCCCCA GGACTCTCAC 
TCCCATTCTT TCCGCAGTGC CATGTACCTG GAGATCATCA TCTCCCTCTG TCTCTACTTC 
CTGAGAAGCT ATTACTCTGC CCATGTGACA TTGGGTCCTC AGGACTTGGC AGGAAACCGG 
GCCATGCAGC TGACCAGCGT GGAGGTCTTG ACACTGCTAT TCAGCGAACT AGCCAAAGTC 
ACAGCTGGCT CGGCAAAGGG TTTTGCTAGC TTCATCAGTG ACGTCCTTTC CAAGTGCAAA 
GTTCAGAAAG TCATCCTCCA TTGCCTGCTT TCCTCCATAT TCAGCGCCCA GAAATGGCAT 
GACCAACGGA CGGCAGGGGT CAACGTGGCC ACGGTGGAGG AAGGTCTCTC GGAGGACAGC 
GTCATCAACA TGTCGGAGGA CCAGATAGAC AGCTGCAGCG CCGTTCAGTC CCAGCTGCTC 
CGGCTGCTGC AGAGTCTAGT TGTACTTGAG CATAGGGTCT TAATGCCAGC AGACGAAGGT 
GGAGAAGGAG GACCTGCCGC AGGAGGAGCA GGTGGAGGGC CAGGAAGCGG TGGAGTGGCA 
GGATTTGAGA TCCTAGGAGG GGAGGTGGAG CATGTTAACC CTCAGCAGCC TATGACGTCA 
CTGCAGTACC TCCATGGTCA GCCTATTACA GCTCAGGGCA TGTTCCTGTG TGCAGTCATC 
AGGGCCCTGC ACCAGCACCA TGCCTGTAAG ATGCACCCCC AATGGATAGG GCTGATCACA 
ACCACCCTGC CTTACATGGG GAGGGTCCTG AGGAGGGTGG TGGCCTCAGT GACTCTGCAG 
CTATGCAGAA ACCTGGATAA TCTCCTCCAG CAGTACCGCT ATGAAACCAG CATAACTGAC 
ACCAGACCCC AGTGGATGGC TCTCTGCATC CCTCCAGACC TGATTCTGAC TGTGCTGGAG 
GGAATCACTT CCATTATCCA TTACTGTCTG CTGGACCCCA CTTCACAGTA CCACCAGTTA 
CAAGTGAGTG TTGACCAGAA GCACCTGGCC GAGGCGCGTT CAGGTATCCT GTCCATCCTC 
CACACCATCA TGTCTTCCGT CACGCTGCTG TGGAGCGTGC TCCACCAAGC CGACAGCTCT 
GACAAGCCAG CTGCCGCTTC AGCTGCCGCC ACCTCAAACA TCAACCTAGG CTCCACCAAG 
AATCTCCGTC AGCAGATCTT GGAACTGCTG GGTCCAATCT CCATGAACCA CGGCGCTCAT 
TTCATGGCTG CAATTGCTTA TGTTTGGAAC GAGAGAAAAC AAGTCAAGGC GCCAATCAGA 
AATAAGGTCA TCCCTGTAGC GAGTGAGGAG CAGCTGCTGC TGGTTGAGCT CGTTCGGTCG 
GTCAGCGCCA TGCGCACTGA GACTGTTATG CAGACAGTGA AGGAGGTCCT GAAGCAGCCA 
CCTGCTATCG CAAAAGACAA GAAGCACATC TCTTTGGAAG TCTGCATGCT ACAGTTCTTC 
TATGCCTATG TACAGAGGAT TCCTGTTTCC AGTTTGCTTG ATAGCTGGCC GTCTCTGCTG 
GCGCTGCTGA AGGACTCGGT CCAGTTGGGT CTGCCTGCTC CTGGGCAGTT TCTCATACTG 
GGAGTTCTGA ATGAGTTTAT CTTGAAGAAT CCCAACCTGG AGAGTAAGAA AGACCAGCGA 
GAGCTGCAGG ATGTGACTCA CAAGGTGGTG GAGGCCATCG GCACCATCGC TGGCTCCTCT 
TTGGAACAAA CCACGTGGCT AAGGAGGAAC CTGGAGGTGA AGGCATCGCC TCAGATTGTT 
GTGGACGGAA CCAACCTGGA GGCAGACGTA GAAGACTTAA TGCTCACAGT GATGGAGGCC 
TCCAGCTTCA CTCCATCTGT GTACAGTGTT CACGCCCTCA CGCTGCTGGC CGAGGTGCTG 
GCTCATCTAT TGGACATGGT GTTCTACAGT GACGAGAAGG AGAAAGTCAT CCCTCTGCTG 
GTTAACATTA TGCACTACGT AGTTCCCTAC CTCCGCAACC ACAGTGCTCA CAATGCCCCC 
AGCTACCGAG CCTGCATCCA GCTGCTGAGC AGCCTGAGCG GCTACCAGTA CACCCGCCGT 
GCCTGGAAAA AAGAGGCCTT CGACCTCTTC ATGGACCACA CCTTCTTCCA GATGGATGCT 
TCGTGTGTCA GCCACTGGAG AGCCATCATT GACCACCTGA TGACTCATGA CAAGACCACA 
TTTAGAGACC TGATGACCCG GGTGGCGGTG GCTCAGAGCA GCTCCCTGAG TTTGTTCACC 
AACAGAGACG CTGAGCTGGA GCAGAGGGCC ATGCTGCTCA AACGCCTGGC CTTCACCATC 
TACAGCAGCG AGGTGGACCA GTACCAGAAG TACCTGCCGG ACATCCAAGA GCGTCTGGTG 
GAGAGCCTGC GTCTGCCTCA GGTACCCATC CTCCACGCTC AGGTGTTCCT GTTCTTCAGA 
GTGCTGCTGC TGCGCATGTC GCCTCAACAC CTCACGTCAC TCTGGCCCAC CATGATCACT 
GAGCTGGTCC AAGTGTTTCT GCTGATGGAG CAGGAGTTAA CCGCTGACGA GGACATATCA 
AGGACGTCAG GACCGTCGGT TGTCGGGTTG GAGACCACCT ACTCTGGAGG GAACGGCTTC 
TCCACGTCCT ACAACAGCCA GCGCTGGCTC AACCTCTACC TCTCTGCTTG CAAGTTCCTG 
GATCTTGCCT TGGCGTTGCC TCCTGAGAGT CTCCCTCAGT TCCAGATGTA CCGCTGGGCC 
TTCATCCCAG AGGCTTCAGA CGATTCAGGG TTGGAAGTGA GACGCCAGGG GACGCATCAG 
AGGGAGTTCA AGCCCTACGT GGTCCGTCTG GTGAAGCTGC TGAGAAAACG AGCCAAGAAA 
AACCCAGAGG AGGACTGTTC CACTAAAACC CTGCCGTGGG AGCCGGGCCA CCTGATGCTG 
ACCCTCTACG TCATCCGCAG CATGGAGCAG CTGCTGCCCT TCTTCAACCT GCTCAGCCAG 
GTCTTCAACA GCAGGGCGAG CTGCCGATCG GGGCCCGTCT ACACCCACAA TCCCGTGGAT 
TCCTCCTTCC CCGCCAGCAA GGAGGGCCAC AAGCTGGAGA GCCAGAAGGT GTTCTGGAGC 
CGAGCCCGAC AGAACATCGA GGAGATGGTG GAAAAAGACT TTCTAGAGGG TCTTATCAAG 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014328228.1
CDS	325..7770
Translation	MNAEEVELLSDSKYRNYVAAVDKALKNFEYSSEWADLISALGKLNKVLQNNAKYQVVPKKLTIGKRLAQCLHPALPSGVHRKALETYEIIFKIIGPKRLAKDLFLYSSGLFPLLSNAAMSVKPVLLGLYETYYLPLGKTLKPGLQGLLTGVLPGLEEGSEYYDREKRSAAATTAHTSALWGSILTSPSVRLPGVSFVLLHLNRKLSMEDQLYVMGSDIELMVEAVSTSVQDSSVLVQRSTLDLILFCFPFHMSQATRPDMIRILSAALHVVLRRDMSLNRRLYAWLLGFDNNGGMSGPRSTRQSNPEEHASHYFNSFSKDMLVQAMVGILQGKARGGEEESILMHDLKPFRILISLLDKPELGPAILEDVLIEVFRTLYTQCRTELDLQNQSPFSKDHTHLSSKLRENKKTAELIKTANLLFNSFEPYYMWDYIARWFEECCRRTLNRSTRVARYGWSLDSPDLSLVEFCQLVDFLLDIVSLPTRSMRVICQETYIEIQTEHLPQLLLRMVAALTCHLQALDLGELTHCLRLCSKILSKVQPPLVSPLALPVSPQPPGLAAQNKSRDVDVKQTPPSVPEMTDSGDVFDDGEIPTGSRSAESGFTDFVQYREDGSEDPERASHPHPPLRAGSRPSGSCQSKPLDKPVMQCCLEHFQQFLSCLITFYIVPEHLDKAEGQRGELLHSGPLVSAGSQPSNYTEPCSGAGVVQKDCLAAFTAACQLFLECSSFPVYIAEGNLKSSPAQEEQCDSEQVRLPVWLQTLMDACCLAGDFSLQGVAISLLMDLIGLTQSVAVVTAESLASSSSTEPTQPMSPSQGRVAVVIRPPLTQGILKYIADKTDFFKSVAVILWDQLGEGTPQHHQRSVELFYQLHNLVPSSSTCEDVISQQLMHRDKRIRLAAHVKFSVLWHLTRDLNITKSSPFSRTFDRSLFIMLDSLSYWDPCTSAVGRAWLNQVLQRHDIARVLEPLLLLLLHPKTHRVSIQKVQAQRHWAQIFPDPPEQEPTEPIYIRDSGFSDNFLQIRSEGGGHEDFRGLTIGDMEPFCLTVNPLSDSLSILSLSSENLHLAGEYQTRDQQEEQQSSKSSGSHSSTLENGSFEDPEGVSYIGNGSDRPLGSSDETPDDSIEEAVSCVVKDLIDRVLNLVDEESNETSSPPENWPQTDTESTSSDTSTGIRLDSCPPQCSNHQNLPQLLAEGTLEFLSVTPAEGLHKEGIARHSSSPSIVMLPDSTDAATPDRSLQVEDPQARKRSHSSTQLSLKGKIMEKLVDKSPGAKPKIKKTKRKEEEKQKKTNQAEKLHPLSIFFGDSLDLENWYSCGEGEVSEIESDMGSPSGGSCGTGAGANVTGRRSSSTLPRFNIHPLYQHVLLYLQLYDSSRTLHALSAIAAMVRAAPSGFVSAISTTSINNTYTPQLSLLQNLLARHRVSVMGKDFYCPIPQDSHSHSFRSAMYLEIIISLCLYFLRSYYSAHVTLGPQDLAGNRAMQLTSVEVLTLLFSELAKVTAGSAKGFASFISDVLSKCKVQKVILHCLLSSIFSAQKWHDQRTAGVNVATVEEGLSEDSVINMSEDQIDSCSAVQSQLLRLLQSLVVLEHRVLMPADEGGEGGPAAGGAGGGPGSGGVAGFEILGGEVEHVNPQQPMTSLQYLHGQPITAQGMFLCAVIRALHQHHACKMHPQWIGLITTTLPYMGRVLRRVVASVTLQLCRNLDNLLQQYRYETSITDTRPQWMALCIPPDLILTVLEGITSIIHYCLLDPTSQYHQLQVSVDQKHLAEARSGILSILHTIMSSVTLLWSVLHQADSSDKPAAASAAATSNINLGSTKNLRQQILELLGPISMNHGAHFMAAIAYVWNERKQVKAPIRNKVIPVASEEQLLLVELVRSVSAMRTETVMQTVKEVLKQPPAIAKDKKHISLEVCMLQFFYAYVQRIPVSSLLDSWPSLLALLKDSVQLGLPAPGQFLILGVLNEFILKNPNLESKKDQRELQDVTHKVVEAIGTIAGSSLEQTTWLRRNLEVKASPQIVVDGTNLEADVEDLMLTVMEASSFTPSVYSVHALTLLAEVLAHLLDMVFYSDEKEKVIPLLVNIMHYVVPYLRNHSAHNAPSYRACIQLLSSLSGYQYTRRAWKKEAFDLFMDHTFFQMDASCVSHWRAIIDHLMTHDKTTFRDLMTRVAVAQSSSLSLFTNRDAELEQRAMLLKRLAFTIYSSEVDQYQKYLPDIQERLVESLRLPQVPILHAQVFLFFRVLLLRMSPQHLTSLWPTMITELVQVFLLMEQELTADEDISRTSGPSVVGLETTYSGGNGFSTSYNSQRWLNLYLSACKFLDLALALPPESLPQFQMYRWAFIPEASDDSGLEVRRQGTHQREFKPYVVRLVKLLRKRAKKNPEEDCSTKTLPWEPGHLMLTLYVIRSMEQLLPFFNLLSQVFNSRASCRSGPVYTHNPVDSSFPASKEGHKLESQKVFWSRARQNIEEMVEKDFLEGLIKT

Target ORF information:

RefSeq Version	XM_014472742.1
Organism	Xiphophorus maculatus(Southern platyfish)
Definition	Xiphophorus maculatus dopey family member 1 (dopey1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014472742.1

ORF Insert Sequence:

ATGAATGCAG AGGAAGTGGA GCTGCTCAGC GACTCAAAGT ACAGGAACTA TGTGGCAGCA 
GTGGACAAAG CCCTCAAGAA CTTTGAGTAT TCCAGCGAGT GGGCCGACCT CATCTCCGCA 
CTGGGCAAGC TCAACAAGGT TTTGCAGAAC AATGCAAAAT ATCAAGTTGT CCCCAAGAAG 
CTGACAATAG GCAAACGGCT GGCCCAGTGC CTCCACCCAG CCCTGCCCAG CGGCGTGCAC 
CGGAAGGCCT TGGAGACCTA CGAGATCATT TTCAAGATCA TTGGGCCCAA GAGGCTGGCC 
AAAGACCTCT TTCTGTACAG CTCGGGACTC TTCCCACTAC TCTCCAACGC TGCTATGTCT 
GTGAAGCCCG TGTTGCTGGG GCTCTATGAG ACCTACTACC TGCCCCTGGG GAAGACTCTC 
AAACCAGGCC TGCAGGGCTT ACTGACCGGC GTGCTGCCTG GTCTGGAGGA AGGTTCAGAG 
TATTACGACA GGTAGGAAAA GAGAAGTGCT GCTGCAACAA CATGAGCGCA CACCAGTGCC 
TTGTGGGGCA GCATCCTGAC CAGCCCCTCT GTGCGTCTCC CCGGGGTCTC CTTCGTTCTG 
CTGCACCTCA ACCGGAAGCT CTCCATGGAG GACCAGCTCT ACGTCATGGG CAGCGACATT 
GAGCTTATGG TGGAGGCTGT TAGTACCTCG GTGCAGGACT CAAGCGTTTT GGTCCAGAGA 
AGCACTCTGG ACCTCATCCT CTTCTGCTTC CCCTTCCATA TGAGCCAGGC TACCCGCCCT 
GACATGATCC GGATCCTTTC AGCAGCTTTG CATGTGGTTC TGAGAAGAGA CATGTCTCTG 
AACCGCAGAC TCTACGCCTG GCTGCTGGGC TTTGACAATA ATGGCGGGAT GTCAGGCCCC 
CGCAGTACTC GGCAGAGCAA CCCGGAGGAA CACGCTTCCC ACTACTTCAA CAGCTTCTCC 
AAGGACATGC TCGTCCAGGC AATGGTTGGG ATTCTGCAGG GCAAGGCTCG AGGTGGGGAG 
GAGGAGAGCA TCCTGATGCA CGACCTCAAG CCATTTCGCA TCCTGATCAG TCTGCTGGAC 
AAACCAGAAC TAGGGCCGGC CATTCTAGAG GACGTTCTGA TCGAAGTGTT TCGGACTCTT 
TACACACAGT GCCGGACAGA GTTGGACCTT CAAAACCAGA GTCCGTTCAG CAAAGATCAC 
ACACACCTCA GCAGTAAACT GCGAGAAAAC AAGAAGACGG CAGAGCTTAT CAAAACAGCC 
AATCTCCTGT TCAACTCGTT TGAGCCGTAC TACATGTGGG ACTACATCGC TCGCTGGTTT 
GAGGAGTGCT GCAGGAGAAC ATTGAACCGC AGCACACGTG TTGCACGATA CGGTTGGAGT 
CTGGATTCTC CTGATCTTTC TCTGGTGGAG TTCTGTCAGT TGGTAGATTT TCTACTAGAC 
ATTGTTTCAT TGCCTACTAG AAGCATGAGG GTAATCTGCC AGGAGACGTA CATAGAAATC 
CAGACGGAGC ATCTCCCTCA ACTGCTGTTG CGGATGGTGG CAGCTCTGAC CTGTCATCTC 
CAGGCGCTGG ACCTCGGGGA GCTCACCCAC TGCCTTCGCC TCTGCTCCAA GATCCTCAGC 
AAGGTGCAGC CTCCGTTGGT GTCCCCGCTC GCCCTCCCCG TGAGTCCACA GCCGCCGGGC 
CTGGCGGCAC AGAACAAAAG CAGAGATGTG GATGTTAAAC AGACTCCTCC CTCTGTCCCA 
GAGATGACCG ACAGTGGGGA CGTGTTTGAC GATGGGGAAA TCCCTACTGG CAGTCGCTCT 
GCAGAAAGTG GCTTCACAGA CTTTGTTCAA TACCGAGAAG ATGGTTCCGA GGATCCAGAG 
CGAGCCTCTC ATCCCCACCC GCCTCTTAGA GCAGGCAGTC GTCCGTCAGG CTCGTGTCAG 
TCCAAGCCTT TGGACAAACC AGTCATGCAG TGTTGCCTGG AGCACTTCCA GCAGTTTCTC 
TCCTGTCTGA TCACTTTTTA TATTGTTCCT GAGCACCTGG ACAAAGCTGA GGGTCAGAGA 
GGTGAGCTCT TGCATTCGGG GCCTTTGGTT TCCGCAGGTT CCCAGCCCAG TAATTATACG 
GAGCCGTGCT CAGGAGCTGG AGTGGTCCAG AAAGATTGTC TGGCTGCATT TACTGCTGCC 
TGTCAGCTCT TCTTAGAATG CTCCAGTTTC CCAGTCTACA TTGCAGAGGG CAACCTCAAG 
TCCTCGCCTG CACAGGAAGA GCAATGCGAC AGTGAGCAGG TTCGGCTGCC AGTGTGGCTG 
CAGACCCTGA TGGATGCCTG CTGCCTGGCT GGTGACTTCA GCCTACAGGG TGTTGCCATC 
TCCCTGCTTA TGGACCTAAT AGGCCTGACT CAGTCAGTTG CTGTGGTGAC GGCAGAGAGT 
CTGGCTTCCA GTAGCAGTAC GGAGCCCACT CAGCCAATGA GCCCCAGCCA GGGTCGGGTG 
GCTGTGGTCA TTAGGCCTCC ACTCACTCAG GGAATCCTGA AGTATATTGC GGATAAAACG 
GACTTCTTCA AGAGCGTTGC AGTGATATTA TGGGACCAGC TGGGTGAAGG AACTCCTCAG 
CATCACCAGC GCAGTGTTGA GCTCTTTTAT CAGCTACACA ACCTGGTCCC TTCATCCAGC 
ACCTGTGAGG ACGTCATAAG CCAGCAACTC ATGCACAGAG ACAAGAGAAT TCGTTTGGCA 
GCCCACGTGA AGTTCTCTGT TCTGTGGCAT TTAACGCGAG ACTTGAACAT CACCAAGTCT 
TCTCCGTTTA GTCGCACATT TGACAGGTCT CTCTTCATCA TGCTCGACAG CTTAAGTTAT 
TGGGATCCAT GTACCAGTGC GGTCGGTCGG GCTTGGTTAA ATCAAGTCCT TCAGAGACAT 
GACATTGCAC GGGTTCTTGA GCCACTCCTA CTTCTTCTGC TCCATCCTAA AACTCACAGG 
GTTTCTATTC AGAAGGTGCA AGCCCAACGC CATTGGGCGC AGATATTTCC TGATCCACCT 
GAGCAGGAAC CAACAGAACC AATCTACATT AGAGACTCCG GCTTCTCAGA CAACTTTCTT 
CAGATTCGAA GCGAAGGGGG TGGGCATGAG GACTTCAGAG GTCTGACAAT AGGTGACATG 
GAGCCATTTT GCCTGACGGT AAACCCCCTA AGTGACAGCT TGTCCATTCT TAGCCTGAGC 
AGTGAAAACT TGCACTTAGC TGGTGAGTAT CAGACCAGGG ACCAGCAGGA GGAGCAACAG 
AGTTCAAAAT CAAGTGGTTC TCATTCATCT ACACTGGAAA ACGGTAGCTT TGAAGACCCT 
GAGGGGGTCA GCTACATCGG AAATGGCTCA GATCGACCGC TTGGTTCATC TGATGAAACG 
CCTGACGACT CCATAGAGGA AGCTGTGTCT TGTGTAGTGA AGGACCTGAT TGATAGGGTT 
CTGAATTTAG TTGATGAGGA ATCAAATGAA ACCTCATCTC CACCTGAAAA TTGGCCCCAA 
ACTGATACTG AAAGCACTTC TTCAGACACC TCCACCGGGA TCCGCCTGGA CTCCTGTCCT 
CCTCAATGCT CCAACCACCA GAATCTGCCA CAGTTATTGG CTGAAGGCAC ACTAGAGTTC 
CTCTCTGTTA CTCCAGCGGA GGGGCTACAC AAAGAGGGCA TCGCCCGCCA CAGTTCATCG 
CCCTCCATCG TCATGTTGCC AGACAGCACT GATGCAGCCA CTCCAGACCG CAGCCTGCAG 
GTGGAGGACC CTCAGGCCCG CAAACGTAGC CACAGCAGCA CTCAGCTTAG CCTTAAAGGG 
AAGATCATGG AGAAGCTGGT AGACAAGTCT CCAGGGGCAA AGCCCAAGAT CAAGAAGACT 
AAGAGGAAAG AGGAAGAGAA GCAGAAAAAA ACGAACCAAG CTGAGAAACT GCATCCACTC 
AGTATTTTCT TTGGAGACAG TCTAGACCTC GAGAACTGGT ACAGCTGTGG TGAAGGGGAG 
GTGTCGGAGA TCGAGAGTGA CATGGGCTCA CCGAGCGGGG GCTCTTGCGG GACCGGTGCT 
GGGGCGAACG TCACAGGGCG CAGATCGTCC TCCACTCTGC CTCGTTTTAA CATCCATCCT 
CTCTACCAGC ACGTCCTGCT CTACCTACAG CTCTACGACT CCTCTAGGAC CCTGCATGCC 
CTGTCGGCGA TCGCAGCCAT GGTGAGAGCC GCTCCCTCAG GCTTTGTAAG CGCCATCTCC 
ACCACCAGCA TCAATAACAC CTACACTCCA CAGCTCTCCT TGCTGCAAAA CCTACTTGCT 
CGCCACAGAG TCTCTGTTAT GGGCAAAGAC TTCTACTGCC CCATTCCCCA GGACTCTCAC 
TCCCATTCTT TCCGCAGTGC CATGTACCTG GAGATCATCA TCTCCCTCTG TCTCTACTTC 
CTGAGAAGCT ATTACTCTGC CCATGTGACA TTGGGTCCTC AGGACTTGGC AGGAAACCGG 
GCCATGCAGC TGACCAGCGT GGAGGTCTTG ACACTGCTAT TCAGCGAACT AGCCAAAGTC 
ACAGCTGGCT CGGCAAAGGG TTTTGCTAGC TTCATCAGTG ACGTCCTTTC CAAGTGCAAA 
GTTCAGAAAG TCATCCTCCA TTGCCTGCTT TCCTCCATAT TCAGCGCCCA GAAATGGCAT 
GACCAACGGA CGGCAGGGGT CAACGTGGCC ACGGTGGAGG AAGGTCTCTC GGAGGACAGC 
GTCATCAACA TGTCGGAGGA CCAGATAGAC AGCTGCAGCG CCGTTCAGTC CCAGCTGCTC 
CGGCTGCTGC AGAGTCTAGT TGTACTTGAG CATAGGGTCT TAATGCCAGC AGACGAAGGT 
GGAGAAGGAG GACCTGCCGC AGGAGGAGCA GGTGGAGGGC CAGGAAGCGG TGGAGTGGCA 
GGATTTGAGA TCCTAGGAGG GGAGGTGGAG CATGTTAACC CTCAGCAGCC TATGACGTCA 
CTGCAGTACC TCCATGGTCA GCCTATTACA GCTCAGGGCA TGTTCCTGTG TGCAGTCATC 
AGGGCCCTGC ACCAGCACCA TGCCTGTAAG ATGCACCCCC AATGGATAGG GCTGATCACA 
ACCACCCTGC CTTACATGGG GAGGGTCCTG AGGAGGGTGG TGGCCTCAGT GACTCTGCAG 
CTATGCAGAA ACCTGGATAA TCTCCTCCAG CAGTACCGCT ATGAAACCAG CATAACTGAC 
ACCAGACCCC AGTGGATGGC TCTCTGCATC CCTCCAGACC TGATTCTGAC TGTGCTGGAG 
GGAATCACTT CCATTATCCA TTACTGTCTG CTGGACCCCA CTTCACAGTA CCACCAGTTA 
CAAGTGAGTG TTGACCAGAA GCACCTGGCC GAGGCGCGTT CAGGTATCCT GTCCATCCTC 
CACACCATCA TGTCTTCCGT CACGCTGCTG TGGAGCGTGC TCCACCAAGC CGACAGCTCT 
GACAAGCCAG CTGCCGCTTC AGCTGCCGCC ACCTCAAACA TCAACCTAGG CTCCACCAAG 
AATCTCCGTC AGCAGATCTT GGAACTGCTG GGTCCAATCT CCATGAACCA CGGCGCTCAT 
TTCATGGCTG CAATTGCTTA TGTTTGGAAC GAGAGAAAAC AAGTCAAGGC GCCAATCAGA 
AATAAGGTCA TCCCTGTAGC GAGTGAGGAG CAGCTGCTGC TGGTTGAGCT CGTTCGGTCG 
GTCAGCGCCA TGCGCACTGA GACTGTTATG CAGACAGTGA AGGAGGTCCT GAAGCAGCCA 
CCTGCTATCG CAAAAGACAA GAAGCACATC TCTTTGGAAG TCTGCATGCT ACAGTTCTTC 
TATGCCTATG TACAGAGGAT TCCTGTTTCC AGTTTGCTTG ATAGCTGGCC GTCTCTGCTG 
GCGCTGCTGA AGGACTCGGT CCAGTTGGGT CTGCCTGCTC CTGGGCAGTT TCTCATACTG 
GGAGTTCTGA ATGAGTTTAT CTTGAAGAAT CCCAACCTGG AGAGTAAGAA AGACCAGCGA 
GAGCTGCAGG ATGTGACTCA CAAGGTGGTG GAGGCCATCG GCACCATCGC TGGCTCCTCT 
TTGGAACAAA CCACGTGGCT AAGGAGGAAC CTGGAGGTGA AGGCATCGCC TCAGATTGTT 
GTGGACGGAA CCAACCTGGA GGCAGACGTA GAAGACTTAA TGCTCACAGT GATGGAGGCC 
TCCAGCTTCA CTCCATCTGT GTACAGTGTT CACGCCCTCA CGCTGCTGGC CGAGGTGCTG 
GCTCATCTAT TGGACATGGT GTTCTACAGT GACGAGAAGG AGAAAGTCAT CCCTCTGCTG 
GTTAACATTA TGCACTACGT AGTTCCCTAC CTCCGCAACC ACAGTGCTCA CAATGCCCCC 
AGCTACCGAG CCTGCATCCA GCTGCTGAGC AGCCTGAGCG GCTACCAGTA CACCCGCCGT 
GCCTGGAAAA AAGAGGCCTT CGACCTCTTC ATGGACCACA CCTTCTTCCA GATGGATGCT 
TCGTGTGTCA GCCACTGGAG AGCCATCATT GACCACCTGA TGACTCATGA CAAGACCACA 
TTTAGAGACC TGATGACCCG GGTGGCGGTG GCTCAGAGCA GCTCCCTGAG TTTGTTCACC 
AACAGAGACG CTGAGCTGGA GCAGAGGGCC ATGCTGCTCA AACGCCTGGC CTTCACCATC 
TACAGCAGCG AGGTGGACCA GTACCAGAAG TACCTGCCGG ACATCCAAGA GCGTCTGGTG 
GAGAGCCTGC GTCTGCCTCA GGTACCCATC CTCCACGCTC AGGTGTTCCT GTTCTTCAGA 
GTGCTGCTGC TGCGCATGTC GCCTCAACAC CTCACGTCAC TCTGGCCCAC CATGATCACT 
GAGCTGGTCC AAGTGTTTCT GCTGATGGAG CAGGAGTTAA CCGCTGACGA GGACATATCA 
AGGACGTCAG GACCGTCGGT TGTCGGGTTG GAGACCACCT ACTCTGGAGG GAACGGCTTC 
TCCACGTCCT ACAACAGCCA GCGCTGGCTC AACCTCTACC TCTCTGCTTG CAAGTTCCTG 
GATCTTGCCT TGGCGTTGCC TCCTGAGAGT CTCCCTCAGT TCCAGATGTA CCGCTGGGCC 
TTCATCCCAG AGGCTTCAGA CGATTCAGGG TTGGAAGTGA GACGCCAGGG GACGCATCAG 
AGGGAGTTCA AGCCCTACGT GGTCCGTCTG GTGAAGCTGC TGAGAAAACG AGCCAAGAAA 
AACCCAGAGG AGGACTGTTC CACTAAAACC CTGCCGTGGG AGCCGGGCCA CCTGATGCTG 
ACCCTCTACG TCATCCGCAG CATGGAGCAG CTGCTGCCCT TCTTCAACCT GCTCAGCCAG 
GTCTTCAACA GCAGGGCGAG CTGCCGATCG GGGCCCGTCT ACACCCACAA TCCCGTGGAT 
TCCTCCTTCC CCGCCAGCAA GGAGGGCCAC AAGCTGGAGA GCCAGAAGGT GTTCTGGAGC 
CGAGCCCGAC AGAACATCGA GGAGATGGTG GAAAAAGACT TTCTAGAGGG TCTTATCAAG 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOPEY1 ( XM_023330621.1 ) cDNA ORF clone, Xiphophorus maculatus(Southern platyfish) -> XP_023186389.1 Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X1, mRNA.

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CloneID	OXi35839
Clone ID Related Accession (Same CDS sequence)	XM_023330621.1 , XM_023330622.1
Accession Version	XM_023330621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	protein dopey-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036445.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAG AGGAAGTGGA GCTGCTCAGC GACTCAAAGT ACAGGAACTA TGTGGCAGCA 
GTGGACAAAG CCCTCAAGAA CTTTGAGTAT TCCAGCGAGT GGGCCGACCT CATCTCCGCA 
CTGGGCAAGC TCAACAAGGT TTTGCAGAAC AATGCAAAAT ATCAAGTTGT CCCCAAGAAG 
CTGACAATAG GCAAACGGCT GGCCCAGTGC CTCCACCCAG CCCTGCCCAG CGGCGTGCAC 
CGGAAGGCCT TGGAGACCTA CGAGATCATT TTCAAGATCA TTGGGCCCAA GAGGCTGGCC 
AAAGACCTCT TTCTGTACAG CTCGGGACTC TTCCCACTAC TCTCCAACGC TGCTATGTCT 
GTGAAGCCCG TGTTGCTGGG GCTCTATGAG ACCTACTACC TGCCCCTGGG GAAGACTCTC 
AAACCAGGCC TGCAGGGCTT ACTGACCGGC GTGCTGCCTG GTCTGGAGGA AGGTTCAGAG 
TATTACGACA GAACCAACAC GCTGTTGGAG AAAGTTGCTG CAGCCGTGGA GCAGTCGGCC 
TTCTACAGTG CCTTGTGGGG CAGCATCCTG ACCAGCCCCT CTGTGCGTCT CCCCGGGGTC 
TCCTTCGTTC TGCTGCACCT CAACCGGAAG CTCTCCATGG AGGACCAGCT CTACGTCATG 
GGCAGCGACA TTGAGCTTAT GGTGGAGGCT GTTAGTACCT CGGTGCAGGA CTCAAGCGTT 
TTGGTCCAGA GAAGCACTCT GGACCTCATC CTCTTCTGCT TCCCCTTCCA TATGAGCCAG 
GCTACCCGCC CTGACATGAT CCGGATCCTT TCAGCAGCTT TGCATGTGGT TCTGAGAAGA 
GACATGTCTC TGAACCGCAG ACTCTACGCC TGGCTGCTGG GCTTTGACAA TAATGGCGGG 
ATGTCAGGCC CCCGCAGTAC TCGGCAGAGC AACCCGGAGG AACACGCTTC CCACTACTTC 
AACAGCTTCT CCAAGGACAT GCTCGTCCAG GCAATGGTTG GGATTCTGCA GGGCAAGGCT 
CGAGGTGGGG AGGAGGAGAG CATCCTGATG CACGACCTCA AGCCATTTCG CATCCTGATC 
AGTCTGCTGG ACAAACCAGA ACTAGGGCCG GCCATTCTAG AGGACGTTCT GATCGAAGTG 
TTTCGGACTC TTTACACACA GTGCCGGACA GAGTTGGACC TTCAAAACCA GAGTCCGTTC 
AGCAAAGATC ACACACACCT CAGCAGTAAA CTGCGAGAAA ACAAGAAGAC GGCAGAGCTT 
ATCAAAACAG CCAATCTCCT GTTCAACTCG TTTGAGCCGT ACTACATGTG GGACTACATC 
GCTCGCTGGT TTGAGGAGTG CTGCAGGAGA ACATTGAACC GCAGCACACG TGTTGCACGA 
TACGGTTGGA GTCTGGATTC TCCTGATCTT TCTCTGGTGG AGTTCTGTCA GTTGGTAGAT 
TTTCTACTAG ACATTGTTTC ATTGCCTACT AGAAGCATGA GGGTAATCTG CCAGGAGACG 
TACATAGAAA TCCAGACGGA GCATCTCCCT CAACTGCTGT TGCGGATGGT GGCAGCTCTG 
ACCTGTCATC TCCAGGCGCT GGACCTCGGG GAGCTCACCC ACTGCCTTCG CCTCTGCTCC 
AAGATCCTCA GCAAGGTGCA GCCTCCGTTG GTGTCCCCGC TCGCCCTCCC CGTGAGTCCA 
CAGCCGCCGG GCCTGGCGGC ACAGAACAAA AGCAGAGATG TGGATGTTAA ACAGACTCCT 
CCCTCTGTCC CAGAGATGAC CGACAGTGGG GACGTGTTTG ACGATGGGGA AATCCCTACT 
GGCAGTCGCT CTGCAGAAAG TGGCTTCACA GACTTTGTTC AATACCGAGA AGATGGTTCC 
GAGGATCCAG AGCGAGCCTC TCATCCCCAC CCGCCTCTTA GAGCAGGCAG TCGTCCGTCA 
GGCTCGTGTC AGTCCAAGCC TTTGGACAAA CCAGTCATGC AGTGTTGCCT GGAGCACTTC 
CAGCAGTTTC TCTCCTGTCT GATCACTTTT TATATTGTTC CTGAGCACCT GGACAAAGCT 
GAGGGTCAGA GAGGTGAGCT CTTGCATTCG GGGCCTTTGG TTTCCGCAGG TTCCCAGCCC 
AGTAATTATA CGGAGCCGTG CTCAGGAGCT GGAGTGGTCC AGAAAGATTG TCTGGCTGCA 
TTTACTGCTG CCTGTCAGCT CTTCTTAGAA TGCTCCAGTT TCCCAGTCTA CATTGCAGAG 
GGCAACCTCA AGTCCTCGCC TGCACAGGAA GAGCAATGCG ACAGTGAGCA GGTTCGGCTG 
CCAGTGTGGC TGCAGACCCT GATGGATGCC TGCTGCCTGG CTGGTGACTT CAGCCTACAG 
GGTGTTGCCA TCTCCCTGCT TATGGACCTA ATAGGCCTGA CTCAGTCAGT TGCTGTGGTG 
ACGGCAGAGA GTCTGGCTTC CAGTAGCAGT ACGGAGCCCA CTCAGCCAAT GAGCCCCAGC 
CAGGGTCGGG TGGCTGTGGT CATTAGGCCT CCACTCACTC AGGGAATCCT GAAGTATATT 
GCGGATAAAA CGGACTTCTT CAAGAGCGTT GCAGTGATAT TATGGGACCA GCTGGGTGAA 
GGAACTCCTC AGCATCACCA GCGCAGTGTT GAGCTCTTTT ATCAGCTACA CAACCTGGTC 
CCTTCATCCA GCACCTGTGA GGACGTCATA AGCCAGCAAC TCATGCACAG AGACAAGAGA 
ATTCGTTTGG CAGCCCACGT GAAGTTCTCT GTTCTGTGGC ATTTAACGCG AGACTTGAAC 
ATCACCAAGT CTTCTCCGTT TAGTCGCACA TTTGACAGGT CTCTCTTCAT CATGCTCGAC 
AGCTTAAGTT ATTGGGATCC ATGTACCAGT GCGGTCGGTC GGGCTTGGTT AAATCAAGTC 
CTTCAGAGAC ATGACATTGC ACGGGTTCTT GAGCCACTCC TACTTCTTCT GCTCCATCCT 
AAAACTCACA GGGTTTCTAT TCAGAAGGTG CAAGCCCAAC GCCATTGGGC GCAGATATTT 
CCTGATCCAC CTGAGCAGGA ACCAACAGAA CCAATCTACA TTAGAGACTC CGGCTTCTCA 
GACAACTTTC TTCAGATTCG AAGCGAAGGG GGTGGGCATG AGGACTTCAG AGGTCTGACA 
ATAGGTGACA TGGAGCCATT TTGCCTGACG GTAAACCCCC TAAGTGACAG CTTGTCCATT 
CTTAGCCTGA GCAGTGAAAA CTTGCACTTA GCTGGTGAGT ATCAGACCAG GGACCAGCAG 
GAGGAGCAAC AGAGTTCAAA ATCAAGTGGT TCTCATTCAT CTACACTGGA AAACGGTAGC 
TTTGAAGACC CTGAGGGGGT CAGCTACATC GGAAATGGCT CAGATCGACC GCTTGGTTCA 
TCTGATGAAA CGCCTGACGA CTCCATAGAG GAAGCTGTGT CTTGTGTAGT GAAGGACCTG 
ATTGATAGGG TTCTGAATTT AGTTGATGAG GAATCAAATG AAACCTCATC TCCACCTGAA 
AATTGGCCCC AAACTGATAC TGAAAGCACT TCTTCAGACA CCTCCACCGG GATCCGCCTG 
GACTCCTGTC CTCCTCAATG CTCCAACCAC CAGAATCTGC CACAGTTATT GGCTGAAGGC 
ACACTAGAGT TCCTCTCTGT TACTCCAGCG GAGGGGCTAC ACAAAGAGGG CATCGCCCGC 
CACAGTTCAT CGCCCTCCAT CGTCATGTTG CCAGACAGCA CTGATGCAGC CACTCCAGAC 
CGCAGCCTGC AGGTGGAGGA CCCTCAGGCC CGCAAACGTA GCCACAGCAG CACTCAGCTT 
AGCCTTAAAG GGAAGATCAT GGAGAAGCTG GTAGACAAGT CTCCAGGGGC AAAGCCCAAG 
ATCAAGAAGA CTAAGAGGAA AGAGGAAGAG AAGCAGAAAA AAACGAACCA AGCTGAGAAA 
CTGCATCCAC TCAGTATTTT CTTTGGAGAC AGTCTAGACC TCGAGAACTG GTACAGCTGT 
GGTGAAGGGG AGGTGTCGGA GATCGAGAGT GACATGGGCT CACCGAGCGG GGGCTCTTGC 
GGGACCGGTG CTGGGGCGAA CGTCACAGGG CGCAGATCGT CCTCCACTCT GCCTCGTTTT 
AACATCCATC CTCTCTACCA GCACGTCCTG CTCTACCTAC AGCTCTACGA CTCCTCTAGG 
ACCCTGCATG CCCTGTCGGC GATCGCAGCC ATGGTGAGAG CCGCTCCCTC AGGCTTTGTA 
AGCGCCATCT CCACCACCAG CATCAATAAC ACCTACACTC CACAGCTCTC CTTGCTGCAA 
AACCTACTTG CTCGCCACAG AGTCTCTGTT ATGGGCAAAG ACTTCTACTG CCCCATTCCC 
CAGGACTCTC ACTCCCATTC TTTCCGCAGT GCCATGTACC TGGAGATCAT CATCTCCCTC 
TGTCTCTACT TCCTGAGAAG CTATTACTCT GCCCATGTGA CATTGGGTCC TCAGGACTTG 
GCAGGAAACC GGGCCATGCA GCTGACCAGC GTGGAGGTCT TGACACTGCT ATTCAGCGAA 
CTAGCCAAAG TCACAGCTGG CTCGGCAAAG GGTTTTGCTA GCTTCATCAG TGACGTCCTT 
TCCAAGTGCA AAGTTCAGAA AGTCATCCTC CATTGCCTGC TTTCCTCCAT ATTCAGCGCC 
CAGAAATGGC ATGACCAACG GACGGCAGGG GTCAACGTGG CCACGGTGGA GGAAGGTCTC 
TCGGAGGACA GCGTCATCAA CATGTCGGAG GACCAGATAG ACAGCTGCAG CGCCGTTCAG 
TCCCAGCTGC TCCGGCTGCT GCAGAGTCTA GTTGTACTTG AGCATAGGGT CTTAATGCCA 
GCAGACGAAG GTGGAGAAGG AGGACCTGCC GCAGGAGGAG CAGGTGGAGG GCCAGGAAGC 
GGTGGAGTGG CAGGATTTGA GATCCTAGGA GGGGAGGTGG AGCATGTTAA CCCTCAGCAG 
CCTATGACGT CACTGCAGTA CCTCCATGGT CAGCCTATTA CAGCTCAGGG CATGTTCCTG 
TGTGCAGTCA TCAGGGCCCT GCACCAGCAC CATGCCTGTA AGATGCACCC CCAATGGATA 
GGGCTGATCA CAACCACCCT GCCTTACATG GGGAGGGTCC TGAGGAGGGT GGTGGCCTCA 
GTGACTCTGC AGCTATGCAG AAACCTGGAT AATCTCCTCC AGCAGTACCG CTATGAAACC 
AGCATAACTG ACACCAGACC CCAGTGGATG GCTCTCTGCA TCCCTCCAGA CCTGATTCTG 
ACTGTGCTGG AGGGAATCAC TTCCATTATC CATTACTGTC TGCTGGACCC CACTTCACAG 
TACCACCAGT TACAAGTGAG TGTTGACCAG AAGCACCTGG CCGAGGCGCG TTCAGGTATC 
CTGTCCATCC TCCACACCAT CATGTCTTCC GTCACGCTGC TGTGGAGCGT GCTCCACCAA 
GCCGACAGCT CTGACAAGCC AGCTGCCGCT TCAGCTGCCG CCACCTCAAA CATCAACCTA 
GGCTCCACCA AGAATCTCCG TCAGCAGATC TTGGAACTGC TGGGTCCAAT CTCCATGAAC 
CACGGCGCTC ATTTCATGGC TGCAATTGCT TATGTTTGGA ACGAGAGAAA ACAAGTCAAG 
GCGCCAATCA GAAATAAGGT CATCCCTGTA GCGAGTGAGG AGCAGCTGCT GCTGGTTGAG 
CTCGTTCGGT CGGTCAGCGC CATGCGCACT GAGACTGTTA TGCAGACAGT GAAGGAGGTC 
CTGAAGCAGC CACCTGCTAT CGCAAAAGAC AAGAAGCACA TCTCTTTGGA AGTCTGCATG 
CTACAGTTCT TCTATGCCTA TGTACAGAGG ATTCCTGTTT CCAGTTTGCT TGATAGCTGG 
CCGTCTCTGC TGGCGCTGCT GAAGGACTCG GTCCAGTTGG GTCTGCCTGC TCCTGGGCAG 
TTTCTCATAC TGGGAGTTCT GAATGAGTTT ATCTTGAAGA ATCCCAACCT GGAGAGTAAG 
AAAGACCAGC GAGAGCTGCA GGATGTGACT CACAAGGTGG TGGAGGCCAT CGGCACCATC 
GCTGGCTCCT CTTTGGAACA AACCACGTGG CTAAGGAGGA ACCTGGAGGT GAAGGCATCG 
CCTCAGATTG TTGTGGACGG AACCAACCTG GAGGCAGACG TAGAAGGTGC TCCGTTACCA 
AACTTAATGC TCACAGTGAT GGAGGCCTCC AGCTTCACTC CATCTGTGTA CAGTGTTCAC 
GCCCTCACGC TGCTGGCCGA GGTGCTGGCT CATCTATTGG ACATGGTGTT CTACAGTGAC 
GAGAAGGAGA AAGTCATCCC TCTGCTGGTT AACATTATGC ACTACGTAGT TCCCTACCTC 
CGCAACCACA GTGCTCACAA TGCCCCCAGC TACCGAGCCT GCATCCAGCT GCTGAGCAGC 
CTGAGCGGCT ACCAGTACAC CCGCCGTGCC TGGAAAAAAG AGGCCTTCGA CCTCTTCATG 
GACCACACCT TCTTCCAGAT GGATGCTTCG TGTGTCAGCC ACTGGAGAGC CATCATTGAC 
CACCTGATGA CTCATGACAA GACCACATTT AGAGACCTGA TGACCCGGGT GGCGGTGGCT 
CAGAGCAGCT CCCTGAGTTT GTTCACCAAC AGAGACGCTG AGCTGGAGCA GAGGGCCATG 
CTGCTCAAAC GCCTGGCCTT CACCATCTAC AGCAGCGAGG TGGACCAGTA CCAGAAGTAC 
CTGCCGGACA TCCAAGAGCG TCTGGTGGAG AGCCTGCGTC TGCCTCAGGT ACCCATCCTC 
CACGCTCAGG TGTTCCTGTT CTTCAGAGTG CTGCTGCTGC GCATGTCGCC TCAACACCTC 
ACGTCACTCT GGCCCACCAT GATCACTGAG CTGGTCCAAG TGTTTCTGCT GATGGAGCAG 
GAGTTAACCG CTGACGAGGA CATATCAAGG ACGTCAGGAC CGTCGGTTGT CGGGTTGGAG 
ACCACCTACT CTGGAGGGAA CGGCTTCTCC ACGTCCTACA ACAGCCAGCG CTGGCTCAAC 
CTCTACCTCT CTGCTTGCAA GTTCCTGGAT CTTGCCTTGG CGTTGCCTCC TGAGAGTCTC 
CCTCAGTTCC AGATGTACCG CTGGGCCTTC ATCCCAGAGG CTTCAGACGA TTCAGGGTTG 
GAAGTGAGAC GCCAGGGGAC GCATCAGAGG GAGTTCAAGC CCTACGTGGT CCGTCTGGTG 
AAGCTGCTGA GAAAACGAGC CAAGAAAAAC CCAGAGGAGG ACTGTTCCAC TAAAACCCTG 
CCGTGGGAGC CGGGCCACCT GATGCTGACC CTCTACGTCA TCCGCAGCAT GGAGCAGCTG 
CTGCCCTTCT TCAACCTGCT CAGCCAGGTC TTCAACAGCA GGGCGAGCTG CCGATCGGGG 
CCCGTCTACA CCCACAATCC CGTGGATTCC TCCTTCCCCG CCAGCAAGGA GGGCCACAAG 
CTGGAGAGCC AGAAGGTGTT CTGGAGCCGA GCCCGACAGA ACATCGAGGA GATGGTGGAA 
AAAGACTTTC TAGAGGGTCT TATCAAGACA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023186389.1
CDS	395..7867
Translation	MNAEEVELLSDSKYRNYVAAVDKALKNFEYSSEWADLISALGKLNKVLQNNAKYQVVPKKLTIGKRLAQCLHPALPSGVHRKALETYEIIFKIIGPKRLAKDLFLYSSGLFPLLSNAAMSVKPVLLGLYETYYLPLGKTLKPGLQGLLTGVLPGLEEGSEYYDRTNTLLEKVAAAVEQSAFYSALWGSILTSPSVRLPGVSFVLLHLNRKLSMEDQLYVMGSDIELMVEAVSTSVQDSSVLVQRSTLDLILFCFPFHMSQATRPDMIRILSAALHVVLRRDMSLNRRLYAWLLGFDNNGGMSGPRSTRQSNPEEHASHYFNSFSKDMLVQAMVGILQGKARGGEEESILMHDLKPFRILISLLDKPELGPAILEDVLIEVFRTLYTQCRTELDLQNQSPFSKDHTHLSSKLRENKKTAELIKTANLLFNSFEPYYMWDYIARWFEECCRRTLNRSTRVARYGWSLDSPDLSLVEFCQLVDFLLDIVSLPTRSMRVICQETYIEIQTEHLPQLLLRMVAALTCHLQALDLGELTHCLRLCSKILSKVQPPLVSPLALPVSPQPPGLAAQNKSRDVDVKQTPPSVPEMTDSGDVFDDGEIPTGSRSAESGFTDFVQYREDGSEDPERASHPHPPLRAGSRPSGSCQSKPLDKPVMQCCLEHFQQFLSCLITFYIVPEHLDKAEGQRGELLHSGPLVSAGSQPSNYTEPCSGAGVVQKDCLAAFTAACQLFLECSSFPVYIAEGNLKSSPAQEEQCDSEQVRLPVWLQTLMDACCLAGDFSLQGVAISLLMDLIGLTQSVAVVTAESLASSSSTEPTQPMSPSQGRVAVVIRPPLTQGILKYIADKTDFFKSVAVILWDQLGEGTPQHHQRSVELFYQLHNLVPSSSTCEDVISQQLMHRDKRIRLAAHVKFSVLWHLTRDLNITKSSPFSRTFDRSLFIMLDSLSYWDPCTSAVGRAWLNQVLQRHDIARVLEPLLLLLLHPKTHRVSIQKVQAQRHWAQIFPDPPEQEPTEPIYIRDSGFSDNFLQIRSEGGGHEDFRGLTIGDMEPFCLTVNPLSDSLSILSLSSENLHLAGEYQTRDQQEEQQSSKSSGSHSSTLENGSFEDPEGVSYIGNGSDRPLGSSDETPDDSIEEAVSCVVKDLIDRVLNLVDEESNETSSPPENWPQTDTESTSSDTSTGIRLDSCPPQCSNHQNLPQLLAEGTLEFLSVTPAEGLHKEGIARHSSSPSIVMLPDSTDAATPDRSLQVEDPQARKRSHSSTQLSLKGKIMEKLVDKSPGAKPKIKKTKRKEEEKQKKTNQAEKLHPLSIFFGDSLDLENWYSCGEGEVSEIESDMGSPSGGSCGTGAGANVTGRRSSSTLPRFNIHPLYQHVLLYLQLYDSSRTLHALSAIAAMVRAAPSGFVSAISTTSINNTYTPQLSLLQNLLARHRVSVMGKDFYCPIPQDSHSHSFRSAMYLEIIISLCLYFLRSYYSAHVTLGPQDLAGNRAMQLTSVEVLTLLFSELAKVTAGSAKGFASFISDVLSKCKVQKVILHCLLSSIFSAQKWHDQRTAGVNVATVEEGLSEDSVINMSEDQIDSCSAVQSQLLRLLQSLVVLEHRVLMPADEGGEGGPAAGGAGGGPGSGGVAGFEILGGEVEHVNPQQPMTSLQYLHGQPITAQGMFLCAVIRALHQHHACKMHPQWIGLITTTLPYMGRVLRRVVASVTLQLCRNLDNLLQQYRYETSITDTRPQWMALCIPPDLILTVLEGITSIIHYCLLDPTSQYHQLQVSVDQKHLAEARSGILSILHTIMSSVTLLWSVLHQADSSDKPAAASAAATSNINLGSTKNLRQQILELLGPISMNHGAHFMAAIAYVWNERKQVKAPIRNKVIPVASEEQLLLVELVRSVSAMRTETVMQTVKEVLKQPPAIAKDKKHISLEVCMLQFFYAYVQRIPVSSLLDSWPSLLALLKDSVQLGLPAPGQFLILGVLNEFILKNPNLESKKDQRELQDVTHKVVEAIGTIAGSSLEQTTWLRRNLEVKASPQIVVDGTNLEADVEGAPLPNLMLTVMEASSFTPSVYSVHALTLLAEVLAHLLDMVFYSDEKEKVIPLLVNIMHYVVPYLRNHSAHNAPSYRACIQLLSSLSGYQYTRRAWKKEAFDLFMDHTFFQMDASCVSHWRAIIDHLMTHDKTTFRDLMTRVAVAQSSSLSLFTNRDAELEQRAMLLKRLAFTIYSSEVDQYQKYLPDIQERLVESLRLPQVPILHAQVFLFFRVLLLRMSPQHLTSLWPTMITELVQVFLLMEQELTADEDISRTSGPSVVGLETTYSGGNGFSTSYNSQRWLNLYLSACKFLDLALALPPESLPQFQMYRWAFIPEASDDSGLEVRRQGTHQREFKPYVVRLVKLLRKRAKKNPEEDCSTKTLPWEPGHLMLTLYVIRSMEQLLPFFNLLSQVFNSRASCRSGPVYTHNPVDSSFPASKEGHKLESQKVFWSRARQNIEEMVEKDFLEGLIKT

Target ORF information:

RefSeq Version	XM_023330621.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023330621.1

ORF Insert Sequence:

ATGAATGCAG AGGAAGTGGA GCTGCTCAGC GACTCAAAGT ACAGGAACTA TGTGGCAGCA 
GTGGACAAAG CCCTCAAGAA CTTTGAGTAT TCCAGCGAGT GGGCCGACCT CATCTCCGCA 
CTGGGCAAGC TCAACAAGGT TTTGCAGAAC AATGCAAAAT ATCAAGTTGT CCCCAAGAAG 
CTGACAATAG GCAAACGGCT GGCCCAGTGC CTCCACCCAG CCCTGCCCAG CGGCGTGCAC 
CGGAAGGCCT TGGAGACCTA CGAGATCATT TTCAAGATCA TTGGGCCCAA GAGGCTGGCC 
AAAGACCTCT TTCTGTACAG CTCGGGACTC TTCCCACTAC TCTCCAACGC TGCTATGTCT 
GTGAAGCCCG TGTTGCTGGG GCTCTATGAG ACCTACTACC TGCCCCTGGG GAAGACTCTC 
AAACCAGGCC TGCAGGGCTT ACTGACCGGC GTGCTGCCTG GTCTGGAGGA AGGTTCAGAG 
TATTACGACA GAACCAACAC GCTGTTGGAG AAAGTTGCTG CAGCCGTGGA GCAGTCGGCC 
TTCTACAGTG CCTTGTGGGG CAGCATCCTG ACCAGCCCCT CTGTGCGTCT CCCCGGGGTC 
TCCTTCGTTC TGCTGCACCT CAACCGGAAG CTCTCCATGG AGGACCAGCT CTACGTCATG 
GGCAGCGACA TTGAGCTTAT GGTGGAGGCT GTTAGTACCT CGGTGCAGGA CTCAAGCGTT 
TTGGTCCAGA GAAGCACTCT GGACCTCATC CTCTTCTGCT TCCCCTTCCA TATGAGCCAG 
GCTACCCGCC CTGACATGAT CCGGATCCTT TCAGCAGCTT TGCATGTGGT TCTGAGAAGA 
GACATGTCTC TGAACCGCAG ACTCTACGCC TGGCTGCTGG GCTTTGACAA TAATGGCGGG 
ATGTCAGGCC CCCGCAGTAC TCGGCAGAGC AACCCGGAGG AACACGCTTC CCACTACTTC 
AACAGCTTCT CCAAGGACAT GCTCGTCCAG GCAATGGTTG GGATTCTGCA GGGCAAGGCT 
CGAGGTGGGG AGGAGGAGAG CATCCTGATG CACGACCTCA AGCCATTTCG CATCCTGATC 
AGTCTGCTGG ACAAACCAGA ACTAGGGCCG GCCATTCTAG AGGACGTTCT GATCGAAGTG 
TTTCGGACTC TTTACACACA GTGCCGGACA GAGTTGGACC TTCAAAACCA GAGTCCGTTC 
AGCAAAGATC ACACACACCT CAGCAGTAAA CTGCGAGAAA ACAAGAAGAC GGCAGAGCTT 
ATCAAAACAG CCAATCTCCT GTTCAACTCG TTTGAGCCGT ACTACATGTG GGACTACATC 
GCTCGCTGGT TTGAGGAGTG CTGCAGGAGA ACATTGAACC GCAGCACACG TGTTGCACGA 
TACGGTTGGA GTCTGGATTC TCCTGATCTT TCTCTGGTGG AGTTCTGTCA GTTGGTAGAT 
TTTCTACTAG ACATTGTTTC ATTGCCTACT AGAAGCATGA GGGTAATCTG CCAGGAGACG 
TACATAGAAA TCCAGACGGA GCATCTCCCT CAACTGCTGT TGCGGATGGT GGCAGCTCTG 
ACCTGTCATC TCCAGGCGCT GGACCTCGGG GAGCTCACCC ACTGCCTTCG CCTCTGCTCC 
AAGATCCTCA GCAAGGTGCA GCCTCCGTTG GTGTCCCCGC TCGCCCTCCC CGTGAGTCCA 
CAGCCGCCGG GCCTGGCGGC ACAGAACAAA AGCAGAGATG TGGATGTTAA ACAGACTCCT 
CCCTCTGTCC CAGAGATGAC CGACAGTGGG GACGTGTTTG ACGATGGGGA AATCCCTACT 
GGCAGTCGCT CTGCAGAAAG TGGCTTCACA GACTTTGTTC AATACCGAGA AGATGGTTCC 
GAGGATCCAG AGCGAGCCTC TCATCCCCAC CCGCCTCTTA GAGCAGGCAG TCGTCCGTCA 
GGCTCGTGTC AGTCCAAGCC TTTGGACAAA CCAGTCATGC AGTGTTGCCT GGAGCACTTC 
CAGCAGTTTC TCTCCTGTCT GATCACTTTT TATATTGTTC CTGAGCACCT GGACAAAGCT 
GAGGGTCAGA GAGGTGAGCT CTTGCATTCG GGGCCTTTGG TTTCCGCAGG TTCCCAGCCC 
AGTAATTATA CGGAGCCGTG CTCAGGAGCT GGAGTGGTCC AGAAAGATTG TCTGGCTGCA 
TTTACTGCTG CCTGTCAGCT CTTCTTAGAA TGCTCCAGTT TCCCAGTCTA CATTGCAGAG 
GGCAACCTCA AGTCCTCGCC TGCACAGGAA GAGCAATGCG ACAGTGAGCA GGTTCGGCTG 
CCAGTGTGGC TGCAGACCCT GATGGATGCC TGCTGCCTGG CTGGTGACTT CAGCCTACAG 
GGTGTTGCCA TCTCCCTGCT TATGGACCTA ATAGGCCTGA CTCAGTCAGT TGCTGTGGTG 
ACGGCAGAGA GTCTGGCTTC CAGTAGCAGT ACGGAGCCCA CTCAGCCAAT GAGCCCCAGC 
CAGGGTCGGG TGGCTGTGGT CATTAGGCCT CCACTCACTC AGGGAATCCT GAAGTATATT 
GCGGATAAAA CGGACTTCTT CAAGAGCGTT GCAGTGATAT TATGGGACCA GCTGGGTGAA 
GGAACTCCTC AGCATCACCA GCGCAGTGTT GAGCTCTTTT ATCAGCTACA CAACCTGGTC 
CCTTCATCCA GCACCTGTGA GGACGTCATA AGCCAGCAAC TCATGCACAG AGACAAGAGA 
ATTCGTTTGG CAGCCCACGT GAAGTTCTCT GTTCTGTGGC ATTTAACGCG AGACTTGAAC 
ATCACCAAGT CTTCTCCGTT TAGTCGCACA TTTGACAGGT CTCTCTTCAT CATGCTCGAC 
AGCTTAAGTT ATTGGGATCC ATGTACCAGT GCGGTCGGTC GGGCTTGGTT AAATCAAGTC 
CTTCAGAGAC ATGACATTGC ACGGGTTCTT GAGCCACTCC TACTTCTTCT GCTCCATCCT 
AAAACTCACA GGGTTTCTAT TCAGAAGGTG CAAGCCCAAC GCCATTGGGC GCAGATATTT 
CCTGATCCAC CTGAGCAGGA ACCAACAGAA CCAATCTACA TTAGAGACTC CGGCTTCTCA 
GACAACTTTC TTCAGATTCG AAGCGAAGGG GGTGGGCATG AGGACTTCAG AGGTCTGACA 
ATAGGTGACA TGGAGCCATT TTGCCTGACG GTAAACCCCC TAAGTGACAG CTTGTCCATT 
CTTAGCCTGA GCAGTGAAAA CTTGCACTTA GCTGGTGAGT ATCAGACCAG GGACCAGCAG 
GAGGAGCAAC AGAGTTCAAA ATCAAGTGGT TCTCATTCAT CTACACTGGA AAACGGTAGC 
TTTGAAGACC CTGAGGGGGT CAGCTACATC GGAAATGGCT CAGATCGACC GCTTGGTTCA 
TCTGATGAAA CGCCTGACGA CTCCATAGAG GAAGCTGTGT CTTGTGTAGT GAAGGACCTG 
ATTGATAGGG TTCTGAATTT AGTTGATGAG GAATCAAATG AAACCTCATC TCCACCTGAA 
AATTGGCCCC AAACTGATAC TGAAAGCACT TCTTCAGACA CCTCCACCGG GATCCGCCTG 
GACTCCTGTC CTCCTCAATG CTCCAACCAC CAGAATCTGC CACAGTTATT GGCTGAAGGC 
ACACTAGAGT TCCTCTCTGT TACTCCAGCG GAGGGGCTAC ACAAAGAGGG CATCGCCCGC 
CACAGTTCAT CGCCCTCCAT CGTCATGTTG CCAGACAGCA CTGATGCAGC CACTCCAGAC 
CGCAGCCTGC AGGTGGAGGA CCCTCAGGCC CGCAAACGTA GCCACAGCAG CACTCAGCTT 
AGCCTTAAAG GGAAGATCAT GGAGAAGCTG GTAGACAAGT CTCCAGGGGC AAAGCCCAAG 
ATCAAGAAGA CTAAGAGGAA AGAGGAAGAG AAGCAGAAAA AAACGAACCA AGCTGAGAAA 
CTGCATCCAC TCAGTATTTT CTTTGGAGAC AGTCTAGACC TCGAGAACTG GTACAGCTGT 
GGTGAAGGGG AGGTGTCGGA GATCGAGAGT GACATGGGCT CACCGAGCGG GGGCTCTTGC 
GGGACCGGTG CTGGGGCGAA CGTCACAGGG CGCAGATCGT CCTCCACTCT GCCTCGTTTT 
AACATCCATC CTCTCTACCA GCACGTCCTG CTCTACCTAC AGCTCTACGA CTCCTCTAGG 
ACCCTGCATG CCCTGTCGGC GATCGCAGCC ATGGTGAGAG CCGCTCCCTC AGGCTTTGTA 
AGCGCCATCT CCACCACCAG CATCAATAAC ACCTACACTC CACAGCTCTC CTTGCTGCAA 
AACCTACTTG CTCGCCACAG AGTCTCTGTT ATGGGCAAAG ACTTCTACTG CCCCATTCCC 
CAGGACTCTC ACTCCCATTC TTTCCGCAGT GCCATGTACC TGGAGATCAT CATCTCCCTC 
TGTCTCTACT TCCTGAGAAG CTATTACTCT GCCCATGTGA CATTGGGTCC TCAGGACTTG 
GCAGGAAACC GGGCCATGCA GCTGACCAGC GTGGAGGTCT TGACACTGCT ATTCAGCGAA 
CTAGCCAAAG TCACAGCTGG CTCGGCAAAG GGTTTTGCTA GCTTCATCAG TGACGTCCTT 
TCCAAGTGCA AAGTTCAGAA AGTCATCCTC CATTGCCTGC TTTCCTCCAT ATTCAGCGCC 
CAGAAATGGC ATGACCAACG GACGGCAGGG GTCAACGTGG CCACGGTGGA GGAAGGTCTC 
TCGGAGGACA GCGTCATCAA CATGTCGGAG GACCAGATAG ACAGCTGCAG CGCCGTTCAG 
TCCCAGCTGC TCCGGCTGCT GCAGAGTCTA GTTGTACTTG AGCATAGGGT CTTAATGCCA 
GCAGACGAAG GTGGAGAAGG AGGACCTGCC GCAGGAGGAG CAGGTGGAGG GCCAGGAAGC 
GGTGGAGTGG CAGGATTTGA GATCCTAGGA GGGGAGGTGG AGCATGTTAA CCCTCAGCAG 
CCTATGACGT CACTGCAGTA CCTCCATGGT CAGCCTATTA CAGCTCAGGG CATGTTCCTG 
TGTGCAGTCA TCAGGGCCCT GCACCAGCAC CATGCCTGTA AGATGCACCC CCAATGGATA 
GGGCTGATCA CAACCACCCT GCCTTACATG GGGAGGGTCC TGAGGAGGGT GGTGGCCTCA 
GTGACTCTGC AGCTATGCAG AAACCTGGAT AATCTCCTCC AGCAGTACCG CTATGAAACC 
AGCATAACTG ACACCAGACC CCAGTGGATG GCTCTCTGCA TCCCTCCAGA CCTGATTCTG 
ACTGTGCTGG AGGGAATCAC TTCCATTATC CATTACTGTC TGCTGGACCC CACTTCACAG 
TACCACCAGT TACAAGTGAG TGTTGACCAG AAGCACCTGG CCGAGGCGCG TTCAGGTATC 
CTGTCCATCC TCCACACCAT CATGTCTTCC GTCACGCTGC TGTGGAGCGT GCTCCACCAA 
GCCGACAGCT CTGACAAGCC AGCTGCCGCT TCAGCTGCCG CCACCTCAAA CATCAACCTA 
GGCTCCACCA AGAATCTCCG TCAGCAGATC TTGGAACTGC TGGGTCCAAT CTCCATGAAC 
CACGGCGCTC ATTTCATGGC TGCAATTGCT TATGTTTGGA ACGAGAGAAA ACAAGTCAAG 
GCGCCAATCA GAAATAAGGT CATCCCTGTA GCGAGTGAGG AGCAGCTGCT GCTGGTTGAG 
CTCGTTCGGT CGGTCAGCGC CATGCGCACT GAGACTGTTA TGCAGACAGT GAAGGAGGTC 
CTGAAGCAGC CACCTGCTAT CGCAAAAGAC AAGAAGCACA TCTCTTTGGA AGTCTGCATG 
CTACAGTTCT TCTATGCCTA TGTACAGAGG ATTCCTGTTT CCAGTTTGCT TGATAGCTGG 
CCGTCTCTGC TGGCGCTGCT GAAGGACTCG GTCCAGTTGG GTCTGCCTGC TCCTGGGCAG 
TTTCTCATAC TGGGAGTTCT GAATGAGTTT ATCTTGAAGA ATCCCAACCT GGAGAGTAAG 
AAAGACCAGC GAGAGCTGCA GGATGTGACT CACAAGGTGG TGGAGGCCAT CGGCACCATC 
GCTGGCTCCT CTTTGGAACA AACCACGTGG CTAAGGAGGA ACCTGGAGGT GAAGGCATCG 
CCTCAGATTG TTGTGGACGG AACCAACCTG GAGGCAGACG TAGAAGGTGC TCCGTTACCA 
AACTTAATGC TCACAGTGAT GGAGGCCTCC AGCTTCACTC CATCTGTGTA CAGTGTTCAC 
GCCCTCACGC TGCTGGCCGA GGTGCTGGCT CATCTATTGG ACATGGTGTT CTACAGTGAC 
GAGAAGGAGA AAGTCATCCC TCTGCTGGTT AACATTATGC ACTACGTAGT TCCCTACCTC 
CGCAACCACA GTGCTCACAA TGCCCCCAGC TACCGAGCCT GCATCCAGCT GCTGAGCAGC 
CTGAGCGGCT ACCAGTACAC CCGCCGTGCC TGGAAAAAAG AGGCCTTCGA CCTCTTCATG 
GACCACACCT TCTTCCAGAT GGATGCTTCG TGTGTCAGCC ACTGGAGAGC CATCATTGAC 
CACCTGATGA CTCATGACAA GACCACATTT AGAGACCTGA TGACCCGGGT GGCGGTGGCT 
CAGAGCAGCT CCCTGAGTTT GTTCACCAAC AGAGACGCTG AGCTGGAGCA GAGGGCCATG 
CTGCTCAAAC GCCTGGCCTT CACCATCTAC AGCAGCGAGG TGGACCAGTA CCAGAAGTAC 
CTGCCGGACA TCCAAGAGCG TCTGGTGGAG AGCCTGCGTC TGCCTCAGGT ACCCATCCTC 
CACGCTCAGG TGTTCCTGTT CTTCAGAGTG CTGCTGCTGC GCATGTCGCC TCAACACCTC 
ACGTCACTCT GGCCCACCAT GATCACTGAG CTGGTCCAAG TGTTTCTGCT GATGGAGCAG 
GAGTTAACCG CTGACGAGGA CATATCAAGG ACGTCAGGAC CGTCGGTTGT CGGGTTGGAG 
ACCACCTACT CTGGAGGGAA CGGCTTCTCC ACGTCCTACA ACAGCCAGCG CTGGCTCAAC 
CTCTACCTCT CTGCTTGCAA GTTCCTGGAT CTTGCCTTGG CGTTGCCTCC TGAGAGTCTC 
CCTCAGTTCC AGATGTACCG CTGGGCCTTC ATCCCAGAGG CTTCAGACGA TTCAGGGTTG 
GAAGTGAGAC GCCAGGGGAC GCATCAGAGG GAGTTCAAGC CCTACGTGGT CCGTCTGGTG 
AAGCTGCTGA GAAAACGAGC CAAGAAAAAC CCAGAGGAGG ACTGTTCCAC TAAAACCCTG 
CCGTGGGAGC CGGGCCACCT GATGCTGACC CTCTACGTCA TCCGCAGCAT GGAGCAGCTG 
CTGCCCTTCT TCAACCTGCT CAGCCAGGTC TTCAACAGCA GGGCGAGCTG CCGATCGGGG 
CCCGTCTACA CCCACAATCC CGTGGATTCC TCCTTCCCCG CCAGCAAGGA GGGCCACAAG 
CTGGAGAGCC AGAAGGTGTT CTGGAGCCGA GCCCGACAGA ACATCGAGGA GATGGTGGAA 
AAAGACTTTC TAGAGGGTCT TATCAAGACA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOPEY1 ( XM_023330622.1 ) cDNA ORF clone, Xiphophorus maculatus(Southern platyfish) -> XP_023186390.1 Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X2, mRNA.

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CloneID	OXi35839
Clone ID Related Accession (Same CDS sequence)	XM_023330621.1 , XM_023330622.1
Accession Version	XM_023330622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	protein dopey-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036445.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAG AGGAAGTGGA GCTGCTCAGC GACTCAAAGT ACAGGAACTA TGTGGCAGCA 
GTGGACAAAG CCCTCAAGAA CTTTGAGTAT TCCAGCGAGT GGGCCGACCT CATCTCCGCA 
CTGGGCAAGC TCAACAAGGT TTTGCAGAAC AATGCAAAAT ATCAAGTTGT CCCCAAGAAG 
CTGACAATAG GCAAACGGCT GGCCCAGTGC CTCCACCCAG CCCTGCCCAG CGGCGTGCAC 
CGGAAGGCCT TGGAGACCTA CGAGATCATT TTCAAGATCA TTGGGCCCAA GAGGCTGGCC 
AAAGACCTCT TTCTGTACAG CTCGGGACTC TTCCCACTAC TCTCCAACGC TGCTATGTCT 
GTGAAGCCCG TGTTGCTGGG GCTCTATGAG ACCTACTACC TGCCCCTGGG GAAGACTCTC 
AAACCAGGCC TGCAGGGCTT ACTGACCGGC GTGCTGCCTG GTCTGGAGGA AGGTTCAGAG 
TATTACGACA GAACCAACAC GCTGTTGGAG AAAGTTGCTG CAGCCGTGGA GCAGTCGGCC 
TTCTACAGTG CCTTGTGGGG CAGCATCCTG ACCAGCCCCT CTGTGCGTCT CCCCGGGGTC 
TCCTTCGTTC TGCTGCACCT CAACCGGAAG CTCTCCATGG AGGACCAGCT CTACGTCATG 
GGCAGCGACA TTGAGCTTAT GGTGGAGGCT GTTAGTACCT CGGTGCAGGA CTCAAGCGTT 
TTGGTCCAGA GAAGCACTCT GGACCTCATC CTCTTCTGCT TCCCCTTCCA TATGAGCCAG 
GCTACCCGCC CTGACATGAT CCGGATCCTT TCAGCAGCTT TGCATGTGGT TCTGAGAAGA 
GACATGTCTC TGAACCGCAG ACTCTACGCC TGGCTGCTGG GCTTTGACAA TAATGGCGGG 
ATGTCAGGCC CCCGCAGTAC TCGGCAGAGC AACCCGGAGG AACACGCTTC CCACTACTTC 
AACAGCTTCT CCAAGGACAT GCTCGTCCAG GCAATGGTTG GGATTCTGCA GGGCAAGGCT 
CGAGGTGGGG AGGAGGAGAG CATCCTGATG CACGACCTCA AGCCATTTCG CATCCTGATC 
AGTCTGCTGG ACAAACCAGA ACTAGGGCCG GCCATTCTAG AGGACGTTCT GATCGAAGTG 
TTTCGGACTC TTTACACACA GTGCCGGACA GAGTTGGACC TTCAAAACCA GAGTCCGTTC 
AGCAAAGATC ACACACACCT CAGCAGTAAA CTGCGAGAAA ACAAGAAGAC GGCAGAGCTT 
ATCAAAACAG CCAATCTCCT GTTCAACTCG TTTGAGCCGT ACTACATGTG GGACTACATC 
GCTCGCTGGT TTGAGGAGTG CTGCAGGAGA ACATTGAACC GCAGCACACG TGTTGCACGA 
TACGGTTGGA GTCTGGATTC TCCTGATCTT TCTCTGGTGG AGTTCTGTCA GTTGGTAGAT 
TTTCTACTAG ACATTGTTTC ATTGCCTACT AGAAGCATGA GGGTAATCTG CCAGGAGACG 
TACATAGAAA TCCAGACGGA GCATCTCCCT CAACTGCTGT TGCGGATGGT GGCAGCTCTG 
ACCTGTCATC TCCAGGCGCT GGACCTCGGG GAGCTCACCC ACTGCCTTCG CCTCTGCTCC 
AAGATCCTCA GCAAGGTGCA GCCTCCGTTG GTGTCCCCGC TCGCCCTCCC CGTGAGTCCA 
CAGCCGCCGG GCCTGGCGGC ACAGAACAAA AGCAGAGATG TGGATGTTAA ACAGACTCCT 
CCCTCTGTCC CAGAGATGAC CGACAGTGGG GACGTGTTTG ACGATGGGGA AATCCCTACT 
GGCAGTCGCT CTGCAGAAAG TGGCTTCACA GACTTTGTTC AATACCGAGA AGATGGTTCC 
GAGGATCCAG AGCGAGCCTC TCATCCCCAC CCGCCTCTTA GAGCAGGCAG TCGTCCGTCA 
GGCTCGTGTC AGTCCAAGCC TTTGGACAAA CCAGTCATGC AGTGTTGCCT GGAGCACTTC 
CAGCAGTTTC TCTCCTGTCT GATCACTTTT TATATTGTTC CTGAGCACCT GGACAAAGCT 
GAGGGTCAGA GAGGTGAGCT CTTGCATTCG GGGCCTTTGG TTTCCGCAGG TTCCCAGCCC 
AGTAATTATA CGGAGCCGTG CTCAGGAGCT GGAGTGGTCC AGAAAGATTG TCTGGCTGCA 
TTTACTGCTG CCTGTCAGCT CTTCTTAGAA TGCTCCAGTT TCCCAGTCTA CATTGCAGAG 
GGCAACCTCA AGTCCTCGCC TGCACAGGAA GAGCAATGCG ACAGTGAGCA GGTTCGGCTG 
CCAGTGTGGC TGCAGACCCT GATGGATGCC TGCTGCCTGG CTGGTGACTT CAGCCTACAG 
GGTGTTGCCA TCTCCCTGCT TATGGACCTA ATAGGCCTGA CTCAGTCAGT TGCTGTGGTG 
ACGGCAGAGA GTCTGGCTTC CAGTAGCAGT ACGGAGCCCA CTCAGCCAAT GAGCCCCAGC 
CAGGGTCGGG TGGCTGTGGT CATTAGGCCT CCACTCACTC AGGGAATCCT GAAGTATATT 
GCGGATAAAA CGGACTTCTT CAAGAGCGTT GCAGTGATAT TATGGGACCA GCTGGGTGAA 
GGAACTCCTC AGCATCACCA GCGCAGTGTT GAGCTCTTTT ATCAGCTACA CAACCTGGTC 
CCTTCATCCA GCACCTGTGA GGACGTCATA AGCCAGCAAC TCATGCACAG AGACAAGAGA 
ATTCGTTTGG CAGCCCACGT GAAGTTCTCT GTTCTGTGGC ATTTAACGCG AGACTTGAAC 
ATCACCAAGT CTTCTCCGTT TAGTCGCACA TTTGACAGGT CTCTCTTCAT CATGCTCGAC 
AGCTTAAGTT ATTGGGATCC ATGTACCAGT GCGGTCGGTC GGGCTTGGTT AAATCAAGTC 
CTTCAGAGAC ATGACATTGC ACGGGTTCTT GAGCCACTCC TACTTCTTCT GCTCCATCCT 
AAAACTCACA GGGTTTCTAT TCAGAAGGTG CAAGCCCAAC GCCATTGGGC GCAGATATTT 
CCTGATCCAC CTGAGCAGGA ACCAACAGAA CCAATCTACA TTAGAGACTC CGGCTTCTCA 
GACAACTTTC TTCAGATTCG AAGCGAAGGG GGTGGGCATG AGGACTTCAG AGGTCTGACA 
ATAGGTGACA TGGAGCCATT TTGCCTGACG GTAAACCCCC TAAGTGACAG CTTGTCCATT 
CTTAGCCTGA GCAGTGAAAA CTTGCACTTA GCTGGTGAGT ATCAGACCAG GGACCAGCAG 
GAGGAGCAAC AGAGTTCAAA ATCAAGTGGT TCTCATTCAT CTACACTGGA AAACGGTAGC 
TTTGAAGACC CTGAGGGGGT CAGCTACATC GGAAATGGCT CAGATCGACC GCTTGGTTCA 
TCTGATGAAA CGCCTGACGA CTCCATAGAG GAAGCTGTGT CTTGTGTAGT GAAGGACCTG 
ATTGATAGGG TTCTGAATTT AGTTGATGAG GAATCAAATG AAACCTCATC TCCACCTGAA 
AATTGGCCCC AAACTGATAC TGAAAGCACT TCTTCAGACA CCTCCACCGG GATCCGCCTG 
GACTCCTGTC CTCCTCAATG CTCCAACCAC CAGAATCTGC CACAGTTATT GGCTGAAGGC 
ACACTAGAGT TCCTCTCTGT TACTCCAGCG GAGGGGCTAC ACAAAGAGGG CATCGCCCGC 
CACAGTTCAT CGCCCTCCAT CGTCATGTTG CCAGACAGCA CTGATGCAGC CACTCCAGAC 
CGCAGCCTGC AGGTGGAGGA CCCTCAGGCC CGCAAACGTA GCCACAGCAG CACTCAGCTT 
AGCCTTAAAG GGAAGATCAT GGAGAAGCTG GTAGACAAGT CTCCAGGGGC AAAGCCCAAG 
ATCAAGAAGA CTAAGAGGAA AGAGGAAGAG AAGCAGAAAA AAACGAACCA AGCTGAGAAA 
CTGCATCCAC TCAGTATTTT CTTTGGAGAC AGTCTAGACC TCGAGAACTG GTACAGCTGT 
GGTGAAGGGG AGGTGTCGGA GATCGAGAGT GACATGGGCT CACCGAGCGG GGGCTCTTGC 
GGGACCGGTG CTGGGGCGAA CGTCACAGGG CGCAGATCGT CCTCCACTCT GCCTCGTTTT 
AACATCCATC CTCTCTACCA GCACGTCCTG CTCTACCTAC AGCTCTACGA CTCCTCTAGG 
ACCCTGCATG CCCTGTCGGC GATCGCAGCC ATGGTGAGAG CCGCTCCCTC AGGCTTTGTA 
AGCGCCATCT CCACCACCAG CATCAATAAC ACCTACACTC CACAGCTCTC CTTGCTGCAA 
AACCTACTTG CTCGCCACAG AGTCTCTGTT ATGGGCAAAG ACTTCTACTG CCCCATTCCC 
CAGGACTCTC ACTCCCATTC TTTCCGCAGT GCCATGTACC TGGAGATCAT CATCTCCCTC 
TGTCTCTACT TCCTGAGAAG CTATTACTCT GCCCATGTGA CATTGGGTCC TCAGGACTTG 
GCAGGAAACC GGGCCATGCA GCTGACCAGC GTGGAGGTCT TGACACTGCT ATTCAGCGAA 
CTAGCCAAAG TCACAGCTGG CTCGGCAAAG GGTTTTGCTA GCTTCATCAG TGACGTCCTT 
TCCAAGTGCA AAGTTCAGAA AGTCATCCTC CATTGCCTGC TTTCCTCCAT ATTCAGCGCC 
CAGAAATGGC ATGACCAACG GACGGCAGGG GTCAACGTGG CCACGGTGGA GGAAGGTCTC 
TCGGAGGACA GCGTCATCAA CATGTCGGAG GACCAGATAG ACAGCTGCAG CGCCGTTCAG 
TCCCAGCTGC TCCGGCTGCT GCAGAGTCTA GTTGTACTTG AGCATAGGGT CTTAATGCCA 
GCAGACGAAG GTGGAGAAGG AGGACCTGCC GCAGGAGGAG CAGGTGGAGG GCCAGGAAGC 
GGTGGAGTGG CAGGATTTGA GATCCTAGGA GGGGAGGTGG AGCATGTTAA CCCTCAGCAG 
CCTATGACGT CACTGCAGTA CCTCCATGGT CAGCCTATTA CAGCTCAGGG CATGTTCCTG 
TGTGCAGTCA TCAGGGCCCT GCACCAGCAC CATGCCTGTA AGATGCACCC CCAATGGATA 
GGGCTGATCA CAACCACCCT GCCTTACATG GGGAGGGTCC TGAGGAGGGT GGTGGCCTCA 
GTGACTCTGC AGCTATGCAG AAACCTGGAT AATCTCCTCC AGCAGTACCG CTATGAAACC 
AGCATAACTG ACACCAGACC CCAGTGGATG GCTCTCTGCA TCCCTCCAGA CCTGATTCTG 
ACTGTGCTGG AGGGAATCAC TTCCATTATC CATTACTGTC TGCTGGACCC CACTTCACAG 
TACCACCAGT TACAAGTGAG TGTTGACCAG AAGCACCTGG CCGAGGCGCG TTCAGGTATC 
CTGTCCATCC TCCACACCAT CATGTCTTCC GTCACGCTGC TGTGGAGCGT GCTCCACCAA 
GCCGACAGCT CTGACAAGCC AGCTGCCGCT TCAGCTGCCG CCACCTCAAA CATCAACCTA 
GGCTCCACCA AGAATCTCCG TCAGCAGATC TTGGAACTGC TGGGTCCAAT CTCCATGAAC 
CACGGCGCTC ATTTCATGGC TGCAATTGCT TATGTTTGGA ACGAGAGAAA ACAAGTCAAG 
GCGCCAATCA GAAATAAGGT CATCCCTGTA GCGAGTGAGG AGCAGCTGCT GCTGGTTGAG 
CTCGTTCGGT CGGTCAGCGC CATGCGCACT GAGACTGTTA TGCAGACAGT GAAGGAGGTC 
CTGAAGCAGC CACCTGCTAT CGCAAAAGAC AAGAAGCACA TCTCTTTGGA AGTCTGCATG 
CTACAGTTCT TCTATGCCTA TGTACAGAGG ATTCCTGTTT CCAGTTTGCT TGATAGCTGG 
CCGTCTCTGC TGGCGCTGCT GAAGGACTCG GTCCAGTTGG GTCTGCCTGC TCCTGGGCAG 
TTTCTCATAC TGGGAGTTCT GAATGAGTTT ATCTTGAAGA ATCCCAACCT GGAGAGTAAG 
AAAGACCAGC GAGAGCTGCA GGATGTGACT CACAAGGTGG TGGAGGCCAT CGGCACCATC 
GCTGGCTCCT CTTTGGAACA AACCACGTGG CTAAGGAGGA ACCTGGAGGT GAAGGCATCG 
CCTCAGATTG TTGTGGACGG AACCAACCTG GAGGCAGACG TAGAAGGTGC TCCGTTACCA 
AACTTAATGC TCACAGTGAT GGAGGCCTCC AGCTTCACTC CATCTGTGTA CAGTGTTCAC 
GCCCTCACGC TGCTGGCCGA GGTGCTGGCT CATCTATTGG ACATGGTGTT CTACAGTGAC 
GAGAAGGAGA AAGTCATCCC TCTGCTGGTT AACATTATGC ACTACGTAGT TCCCTACCTC 
CGCAACCACA GTGCTCACAA TGCCCCCAGC TACCGAGCCT GCATCCAGCT GCTGAGCAGC 
CTGAGCGGCT ACCAGTACAC CCGCCGTGCC TGGAAAAAAG AGGCCTTCGA CCTCTTCATG 
GACCACACCT TCTTCCAGAT GGATGCTTCG TGTGTCAGCC ACTGGAGAGC CATCATTGAC 
CACCTGATGA CTCATGACAA GACCACATTT AGAGACCTGA TGACCCGGGT GGCGGTGGCT 
CAGAGCAGCT CCCTGAGTTT GTTCACCAAC AGAGACGCTG AGCTGGAGCA GAGGGCCATG 
CTGCTCAAAC GCCTGGCCTT CACCATCTAC AGCAGCGAGG TGGACCAGTA CCAGAAGTAC 
CTGCCGGACA TCCAAGAGCG TCTGGTGGAG AGCCTGCGTC TGCCTCAGGT ACCCATCCTC 
CACGCTCAGG TGTTCCTGTT CTTCAGAGTG CTGCTGCTGC GCATGTCGCC TCAACACCTC 
ACGTCACTCT GGCCCACCAT GATCACTGAG CTGGTCCAAG TGTTTCTGCT GATGGAGCAG 
GAGTTAACCG CTGACGAGGA CATATCAAGG ACGTCAGGAC CGTCGGTTGT CGGGTTGGAG 
ACCACCTACT CTGGAGGGAA CGGCTTCTCC ACGTCCTACA ACAGCCAGCG CTGGCTCAAC 
CTCTACCTCT CTGCTTGCAA GTTCCTGGAT CTTGCCTTGG CGTTGCCTCC TGAGAGTCTC 
CCTCAGTTCC AGATGTACCG CTGGGCCTTC ATCCCAGAGG CTTCAGACGA TTCAGGGTTG 
GAAGTGAGAC GCCAGGGGAC GCATCAGAGG GAGTTCAAGC CCTACGTGGT CCGTCTGGTG 
AAGCTGCTGA GAAAACGAGC CAAGAAAAAC CCAGAGGAGG ACTGTTCCAC TAAAACCCTG 
CCGTGGGAGC CGGGCCACCT GATGCTGACC CTCTACGTCA TCCGCAGCAT GGAGCAGCTG 
CTGCCCTTCT TCAACCTGCT CAGCCAGGTC TTCAACAGCA GGGCGAGCTG CCGATCGGGG 
CCCGTCTACA CCCACAATCC CGTGGATTCC TCCTTCCCCG CCAGCAAGGA GGGCCACAAG 
CTGGAGAGCC AGAAGGTGTT CTGGAGCCGA GCCCGACAGA ACATCGAGGA GATGGTGGAA 
AAAGACTTTC TAGAGGGTCT TATCAAGACA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023186390.1
CDS	466..7938
Translation	MNAEEVELLSDSKYRNYVAAVDKALKNFEYSSEWADLISALGKLNKVLQNNAKYQVVPKKLTIGKRLAQCLHPALPSGVHRKALETYEIIFKIIGPKRLAKDLFLYSSGLFPLLSNAAMSVKPVLLGLYETYYLPLGKTLKPGLQGLLTGVLPGLEEGSEYYDRTNTLLEKVAAAVEQSAFYSALWGSILTSPSVRLPGVSFVLLHLNRKLSMEDQLYVMGSDIELMVEAVSTSVQDSSVLVQRSTLDLILFCFPFHMSQATRPDMIRILSAALHVVLRRDMSLNRRLYAWLLGFDNNGGMSGPRSTRQSNPEEHASHYFNSFSKDMLVQAMVGILQGKARGGEEESILMHDLKPFRILISLLDKPELGPAILEDVLIEVFRTLYTQCRTELDLQNQSPFSKDHTHLSSKLRENKKTAELIKTANLLFNSFEPYYMWDYIARWFEECCRRTLNRSTRVARYGWSLDSPDLSLVEFCQLVDFLLDIVSLPTRSMRVICQETYIEIQTEHLPQLLLRMVAALTCHLQALDLGELTHCLRLCSKILSKVQPPLVSPLALPVSPQPPGLAAQNKSRDVDVKQTPPSVPEMTDSGDVFDDGEIPTGSRSAESGFTDFVQYREDGSEDPERASHPHPPLRAGSRPSGSCQSKPLDKPVMQCCLEHFQQFLSCLITFYIVPEHLDKAEGQRGELLHSGPLVSAGSQPSNYTEPCSGAGVVQKDCLAAFTAACQLFLECSSFPVYIAEGNLKSSPAQEEQCDSEQVRLPVWLQTLMDACCLAGDFSLQGVAISLLMDLIGLTQSVAVVTAESLASSSSTEPTQPMSPSQGRVAVVIRPPLTQGILKYIADKTDFFKSVAVILWDQLGEGTPQHHQRSVELFYQLHNLVPSSSTCEDVISQQLMHRDKRIRLAAHVKFSVLWHLTRDLNITKSSPFSRTFDRSLFIMLDSLSYWDPCTSAVGRAWLNQVLQRHDIARVLEPLLLLLLHPKTHRVSIQKVQAQRHWAQIFPDPPEQEPTEPIYIRDSGFSDNFLQIRSEGGGHEDFRGLTIGDMEPFCLTVNPLSDSLSILSLSSENLHLAGEYQTRDQQEEQQSSKSSGSHSSTLENGSFEDPEGVSYIGNGSDRPLGSSDETPDDSIEEAVSCVVKDLIDRVLNLVDEESNETSSPPENWPQTDTESTSSDTSTGIRLDSCPPQCSNHQNLPQLLAEGTLEFLSVTPAEGLHKEGIARHSSSPSIVMLPDSTDAATPDRSLQVEDPQARKRSHSSTQLSLKGKIMEKLVDKSPGAKPKIKKTKRKEEEKQKKTNQAEKLHPLSIFFGDSLDLENWYSCGEGEVSEIESDMGSPSGGSCGTGAGANVTGRRSSSTLPRFNIHPLYQHVLLYLQLYDSSRTLHALSAIAAMVRAAPSGFVSAISTTSINNTYTPQLSLLQNLLARHRVSVMGKDFYCPIPQDSHSHSFRSAMYLEIIISLCLYFLRSYYSAHVTLGPQDLAGNRAMQLTSVEVLTLLFSELAKVTAGSAKGFASFISDVLSKCKVQKVILHCLLSSIFSAQKWHDQRTAGVNVATVEEGLSEDSVINMSEDQIDSCSAVQSQLLRLLQSLVVLEHRVLMPADEGGEGGPAAGGAGGGPGSGGVAGFEILGGEVEHVNPQQPMTSLQYLHGQPITAQGMFLCAVIRALHQHHACKMHPQWIGLITTTLPYMGRVLRRVVASVTLQLCRNLDNLLQQYRYETSITDTRPQWMALCIPPDLILTVLEGITSIIHYCLLDPTSQYHQLQVSVDQKHLAEARSGILSILHTIMSSVTLLWSVLHQADSSDKPAAASAAATSNINLGSTKNLRQQILELLGPISMNHGAHFMAAIAYVWNERKQVKAPIRNKVIPVASEEQLLLVELVRSVSAMRTETVMQTVKEVLKQPPAIAKDKKHISLEVCMLQFFYAYVQRIPVSSLLDSWPSLLALLKDSVQLGLPAPGQFLILGVLNEFILKNPNLESKKDQRELQDVTHKVVEAIGTIAGSSLEQTTWLRRNLEVKASPQIVVDGTNLEADVEGAPLPNLMLTVMEASSFTPSVYSVHALTLLAEVLAHLLDMVFYSDEKEKVIPLLVNIMHYVVPYLRNHSAHNAPSYRACIQLLSSLSGYQYTRRAWKKEAFDLFMDHTFFQMDASCVSHWRAIIDHLMTHDKTTFRDLMTRVAVAQSSSLSLFTNRDAELEQRAMLLKRLAFTIYSSEVDQYQKYLPDIQERLVESLRLPQVPILHAQVFLFFRVLLLRMSPQHLTSLWPTMITELVQVFLLMEQELTADEDISRTSGPSVVGLETTYSGGNGFSTSYNSQRWLNLYLSACKFLDLALALPPESLPQFQMYRWAFIPEASDDSGLEVRRQGTHQREFKPYVVRLVKLLRKRAKKNPEEDCSTKTLPWEPGHLMLTLYVIRSMEQLLPFFNLLSQVFNSRASCRSGPVYTHNPVDSSFPASKEGHKLESQKVFWSRARQNIEEMVEKDFLEGLIKT

Target ORF information:

RefSeq Version	XM_023330622.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023330622.1

ORF Insert Sequence:

ATGAATGCAG AGGAAGTGGA GCTGCTCAGC GACTCAAAGT ACAGGAACTA TGTGGCAGCA 
GTGGACAAAG CCCTCAAGAA CTTTGAGTAT TCCAGCGAGT GGGCCGACCT CATCTCCGCA 
CTGGGCAAGC TCAACAAGGT TTTGCAGAAC AATGCAAAAT ATCAAGTTGT CCCCAAGAAG 
CTGACAATAG GCAAACGGCT GGCCCAGTGC CTCCACCCAG CCCTGCCCAG CGGCGTGCAC 
CGGAAGGCCT TGGAGACCTA CGAGATCATT TTCAAGATCA TTGGGCCCAA GAGGCTGGCC 
AAAGACCTCT TTCTGTACAG CTCGGGACTC TTCCCACTAC TCTCCAACGC TGCTATGTCT 
GTGAAGCCCG TGTTGCTGGG GCTCTATGAG ACCTACTACC TGCCCCTGGG GAAGACTCTC 
AAACCAGGCC TGCAGGGCTT ACTGACCGGC GTGCTGCCTG GTCTGGAGGA AGGTTCAGAG 
TATTACGACA GAACCAACAC GCTGTTGGAG AAAGTTGCTG CAGCCGTGGA GCAGTCGGCC 
TTCTACAGTG CCTTGTGGGG CAGCATCCTG ACCAGCCCCT CTGTGCGTCT CCCCGGGGTC 
TCCTTCGTTC TGCTGCACCT CAACCGGAAG CTCTCCATGG AGGACCAGCT CTACGTCATG 
GGCAGCGACA TTGAGCTTAT GGTGGAGGCT GTTAGTACCT CGGTGCAGGA CTCAAGCGTT 
TTGGTCCAGA GAAGCACTCT GGACCTCATC CTCTTCTGCT TCCCCTTCCA TATGAGCCAG 
GCTACCCGCC CTGACATGAT CCGGATCCTT TCAGCAGCTT TGCATGTGGT TCTGAGAAGA 
GACATGTCTC TGAACCGCAG ACTCTACGCC TGGCTGCTGG GCTTTGACAA TAATGGCGGG 
ATGTCAGGCC CCCGCAGTAC TCGGCAGAGC AACCCGGAGG AACACGCTTC CCACTACTTC 
AACAGCTTCT CCAAGGACAT GCTCGTCCAG GCAATGGTTG GGATTCTGCA GGGCAAGGCT 
CGAGGTGGGG AGGAGGAGAG CATCCTGATG CACGACCTCA AGCCATTTCG CATCCTGATC 
AGTCTGCTGG ACAAACCAGA ACTAGGGCCG GCCATTCTAG AGGACGTTCT GATCGAAGTG 
TTTCGGACTC TTTACACACA GTGCCGGACA GAGTTGGACC TTCAAAACCA GAGTCCGTTC 
AGCAAAGATC ACACACACCT CAGCAGTAAA CTGCGAGAAA ACAAGAAGAC GGCAGAGCTT 
ATCAAAACAG CCAATCTCCT GTTCAACTCG TTTGAGCCGT ACTACATGTG GGACTACATC 
GCTCGCTGGT TTGAGGAGTG CTGCAGGAGA ACATTGAACC GCAGCACACG TGTTGCACGA 
TACGGTTGGA GTCTGGATTC TCCTGATCTT TCTCTGGTGG AGTTCTGTCA GTTGGTAGAT 
TTTCTACTAG ACATTGTTTC ATTGCCTACT AGAAGCATGA GGGTAATCTG CCAGGAGACG 
TACATAGAAA TCCAGACGGA GCATCTCCCT CAACTGCTGT TGCGGATGGT GGCAGCTCTG 
ACCTGTCATC TCCAGGCGCT GGACCTCGGG GAGCTCACCC ACTGCCTTCG CCTCTGCTCC 
AAGATCCTCA GCAAGGTGCA GCCTCCGTTG GTGTCCCCGC TCGCCCTCCC CGTGAGTCCA 
CAGCCGCCGG GCCTGGCGGC ACAGAACAAA AGCAGAGATG TGGATGTTAA ACAGACTCCT 
CCCTCTGTCC CAGAGATGAC CGACAGTGGG GACGTGTTTG ACGATGGGGA AATCCCTACT 
GGCAGTCGCT CTGCAGAAAG TGGCTTCACA GACTTTGTTC AATACCGAGA AGATGGTTCC 
GAGGATCCAG AGCGAGCCTC TCATCCCCAC CCGCCTCTTA GAGCAGGCAG TCGTCCGTCA 
GGCTCGTGTC AGTCCAAGCC TTTGGACAAA CCAGTCATGC AGTGTTGCCT GGAGCACTTC 
CAGCAGTTTC TCTCCTGTCT GATCACTTTT TATATTGTTC CTGAGCACCT GGACAAAGCT 
GAGGGTCAGA GAGGTGAGCT CTTGCATTCG GGGCCTTTGG TTTCCGCAGG TTCCCAGCCC 
AGTAATTATA CGGAGCCGTG CTCAGGAGCT GGAGTGGTCC AGAAAGATTG TCTGGCTGCA 
TTTACTGCTG CCTGTCAGCT CTTCTTAGAA TGCTCCAGTT TCCCAGTCTA CATTGCAGAG 
GGCAACCTCA AGTCCTCGCC TGCACAGGAA GAGCAATGCG ACAGTGAGCA GGTTCGGCTG 
CCAGTGTGGC TGCAGACCCT GATGGATGCC TGCTGCCTGG CTGGTGACTT CAGCCTACAG 
GGTGTTGCCA TCTCCCTGCT TATGGACCTA ATAGGCCTGA CTCAGTCAGT TGCTGTGGTG 
ACGGCAGAGA GTCTGGCTTC CAGTAGCAGT ACGGAGCCCA CTCAGCCAAT GAGCCCCAGC 
CAGGGTCGGG TGGCTGTGGT CATTAGGCCT CCACTCACTC AGGGAATCCT GAAGTATATT 
GCGGATAAAA CGGACTTCTT CAAGAGCGTT GCAGTGATAT TATGGGACCA GCTGGGTGAA 
GGAACTCCTC AGCATCACCA GCGCAGTGTT GAGCTCTTTT ATCAGCTACA CAACCTGGTC 
CCTTCATCCA GCACCTGTGA GGACGTCATA AGCCAGCAAC TCATGCACAG AGACAAGAGA 
ATTCGTTTGG CAGCCCACGT GAAGTTCTCT GTTCTGTGGC ATTTAACGCG AGACTTGAAC 
ATCACCAAGT CTTCTCCGTT TAGTCGCACA TTTGACAGGT CTCTCTTCAT CATGCTCGAC 
AGCTTAAGTT ATTGGGATCC ATGTACCAGT GCGGTCGGTC GGGCTTGGTT AAATCAAGTC 
CTTCAGAGAC ATGACATTGC ACGGGTTCTT GAGCCACTCC TACTTCTTCT GCTCCATCCT 
AAAACTCACA GGGTTTCTAT TCAGAAGGTG CAAGCCCAAC GCCATTGGGC GCAGATATTT 
CCTGATCCAC CTGAGCAGGA ACCAACAGAA CCAATCTACA TTAGAGACTC CGGCTTCTCA 
GACAACTTTC TTCAGATTCG AAGCGAAGGG GGTGGGCATG AGGACTTCAG AGGTCTGACA 
ATAGGTGACA TGGAGCCATT TTGCCTGACG GTAAACCCCC TAAGTGACAG CTTGTCCATT 
CTTAGCCTGA GCAGTGAAAA CTTGCACTTA GCTGGTGAGT ATCAGACCAG GGACCAGCAG 
GAGGAGCAAC AGAGTTCAAA ATCAAGTGGT TCTCATTCAT CTACACTGGA AAACGGTAGC 
TTTGAAGACC CTGAGGGGGT CAGCTACATC GGAAATGGCT CAGATCGACC GCTTGGTTCA 
TCTGATGAAA CGCCTGACGA CTCCATAGAG GAAGCTGTGT CTTGTGTAGT GAAGGACCTG 
ATTGATAGGG TTCTGAATTT AGTTGATGAG GAATCAAATG AAACCTCATC TCCACCTGAA 
AATTGGCCCC AAACTGATAC TGAAAGCACT TCTTCAGACA CCTCCACCGG GATCCGCCTG 
GACTCCTGTC CTCCTCAATG CTCCAACCAC CAGAATCTGC CACAGTTATT GGCTGAAGGC 
ACACTAGAGT TCCTCTCTGT TACTCCAGCG GAGGGGCTAC ACAAAGAGGG CATCGCCCGC 
CACAGTTCAT CGCCCTCCAT CGTCATGTTG CCAGACAGCA CTGATGCAGC CACTCCAGAC 
CGCAGCCTGC AGGTGGAGGA CCCTCAGGCC CGCAAACGTA GCCACAGCAG CACTCAGCTT 
AGCCTTAAAG GGAAGATCAT GGAGAAGCTG GTAGACAAGT CTCCAGGGGC AAAGCCCAAG 
ATCAAGAAGA CTAAGAGGAA AGAGGAAGAG AAGCAGAAAA AAACGAACCA AGCTGAGAAA 
CTGCATCCAC TCAGTATTTT CTTTGGAGAC AGTCTAGACC TCGAGAACTG GTACAGCTGT 
GGTGAAGGGG AGGTGTCGGA GATCGAGAGT GACATGGGCT CACCGAGCGG GGGCTCTTGC 
GGGACCGGTG CTGGGGCGAA CGTCACAGGG CGCAGATCGT CCTCCACTCT GCCTCGTTTT 
AACATCCATC CTCTCTACCA GCACGTCCTG CTCTACCTAC AGCTCTACGA CTCCTCTAGG 
ACCCTGCATG CCCTGTCGGC GATCGCAGCC ATGGTGAGAG CCGCTCCCTC AGGCTTTGTA 
AGCGCCATCT CCACCACCAG CATCAATAAC ACCTACACTC CACAGCTCTC CTTGCTGCAA 
AACCTACTTG CTCGCCACAG AGTCTCTGTT ATGGGCAAAG ACTTCTACTG CCCCATTCCC 
CAGGACTCTC ACTCCCATTC TTTCCGCAGT GCCATGTACC TGGAGATCAT CATCTCCCTC 
TGTCTCTACT TCCTGAGAAG CTATTACTCT GCCCATGTGA CATTGGGTCC TCAGGACTTG 
GCAGGAAACC GGGCCATGCA GCTGACCAGC GTGGAGGTCT TGACACTGCT ATTCAGCGAA 
CTAGCCAAAG TCACAGCTGG CTCGGCAAAG GGTTTTGCTA GCTTCATCAG TGACGTCCTT 
TCCAAGTGCA AAGTTCAGAA AGTCATCCTC CATTGCCTGC TTTCCTCCAT ATTCAGCGCC 
CAGAAATGGC ATGACCAACG GACGGCAGGG GTCAACGTGG CCACGGTGGA GGAAGGTCTC 
TCGGAGGACA GCGTCATCAA CATGTCGGAG GACCAGATAG ACAGCTGCAG CGCCGTTCAG 
TCCCAGCTGC TCCGGCTGCT GCAGAGTCTA GTTGTACTTG AGCATAGGGT CTTAATGCCA 
GCAGACGAAG GTGGAGAAGG AGGACCTGCC GCAGGAGGAG CAGGTGGAGG GCCAGGAAGC 
GGTGGAGTGG CAGGATTTGA GATCCTAGGA GGGGAGGTGG AGCATGTTAA CCCTCAGCAG 
CCTATGACGT CACTGCAGTA CCTCCATGGT CAGCCTATTA CAGCTCAGGG CATGTTCCTG 
TGTGCAGTCA TCAGGGCCCT GCACCAGCAC CATGCCTGTA AGATGCACCC CCAATGGATA 
GGGCTGATCA CAACCACCCT GCCTTACATG GGGAGGGTCC TGAGGAGGGT GGTGGCCTCA 
GTGACTCTGC AGCTATGCAG AAACCTGGAT AATCTCCTCC AGCAGTACCG CTATGAAACC 
AGCATAACTG ACACCAGACC CCAGTGGATG GCTCTCTGCA TCCCTCCAGA CCTGATTCTG 
ACTGTGCTGG AGGGAATCAC TTCCATTATC CATTACTGTC TGCTGGACCC CACTTCACAG 
TACCACCAGT TACAAGTGAG TGTTGACCAG AAGCACCTGG CCGAGGCGCG TTCAGGTATC 
CTGTCCATCC TCCACACCAT CATGTCTTCC GTCACGCTGC TGTGGAGCGT GCTCCACCAA 
GCCGACAGCT CTGACAAGCC AGCTGCCGCT TCAGCTGCCG CCACCTCAAA CATCAACCTA 
GGCTCCACCA AGAATCTCCG TCAGCAGATC TTGGAACTGC TGGGTCCAAT CTCCATGAAC 
CACGGCGCTC ATTTCATGGC TGCAATTGCT TATGTTTGGA ACGAGAGAAA ACAAGTCAAG 
GCGCCAATCA GAAATAAGGT CATCCCTGTA GCGAGTGAGG AGCAGCTGCT GCTGGTTGAG 
CTCGTTCGGT CGGTCAGCGC CATGCGCACT GAGACTGTTA TGCAGACAGT GAAGGAGGTC 
CTGAAGCAGC CACCTGCTAT CGCAAAAGAC AAGAAGCACA TCTCTTTGGA AGTCTGCATG 
CTACAGTTCT TCTATGCCTA TGTACAGAGG ATTCCTGTTT CCAGTTTGCT TGATAGCTGG 
CCGTCTCTGC TGGCGCTGCT GAAGGACTCG GTCCAGTTGG GTCTGCCTGC TCCTGGGCAG 
TTTCTCATAC TGGGAGTTCT GAATGAGTTT ATCTTGAAGA ATCCCAACCT GGAGAGTAAG 
AAAGACCAGC GAGAGCTGCA GGATGTGACT CACAAGGTGG TGGAGGCCAT CGGCACCATC 
GCTGGCTCCT CTTTGGAACA AACCACGTGG CTAAGGAGGA ACCTGGAGGT GAAGGCATCG 
CCTCAGATTG TTGTGGACGG AACCAACCTG GAGGCAGACG TAGAAGGTGC TCCGTTACCA 
AACTTAATGC TCACAGTGAT GGAGGCCTCC AGCTTCACTC CATCTGTGTA CAGTGTTCAC 
GCCCTCACGC TGCTGGCCGA GGTGCTGGCT CATCTATTGG ACATGGTGTT CTACAGTGAC 
GAGAAGGAGA AAGTCATCCC TCTGCTGGTT AACATTATGC ACTACGTAGT TCCCTACCTC 
CGCAACCACA GTGCTCACAA TGCCCCCAGC TACCGAGCCT GCATCCAGCT GCTGAGCAGC 
CTGAGCGGCT ACCAGTACAC CCGCCGTGCC TGGAAAAAAG AGGCCTTCGA CCTCTTCATG 
GACCACACCT TCTTCCAGAT GGATGCTTCG TGTGTCAGCC ACTGGAGAGC CATCATTGAC 
CACCTGATGA CTCATGACAA GACCACATTT AGAGACCTGA TGACCCGGGT GGCGGTGGCT 
CAGAGCAGCT CCCTGAGTTT GTTCACCAAC AGAGACGCTG AGCTGGAGCA GAGGGCCATG 
CTGCTCAAAC GCCTGGCCTT CACCATCTAC AGCAGCGAGG TGGACCAGTA CCAGAAGTAC 
CTGCCGGACA TCCAAGAGCG TCTGGTGGAG AGCCTGCGTC TGCCTCAGGT ACCCATCCTC 
CACGCTCAGG TGTTCCTGTT CTTCAGAGTG CTGCTGCTGC GCATGTCGCC TCAACACCTC 
ACGTCACTCT GGCCCACCAT GATCACTGAG CTGGTCCAAG TGTTTCTGCT GATGGAGCAG 
GAGTTAACCG CTGACGAGGA CATATCAAGG ACGTCAGGAC CGTCGGTTGT CGGGTTGGAG 
ACCACCTACT CTGGAGGGAA CGGCTTCTCC ACGTCCTACA ACAGCCAGCG CTGGCTCAAC 
CTCTACCTCT CTGCTTGCAA GTTCCTGGAT CTTGCCTTGG CGTTGCCTCC TGAGAGTCTC 
CCTCAGTTCC AGATGTACCG CTGGGCCTTC ATCCCAGAGG CTTCAGACGA TTCAGGGTTG 
GAAGTGAGAC GCCAGGGGAC GCATCAGAGG GAGTTCAAGC CCTACGTGGT CCGTCTGGTG 
AAGCTGCTGA GAAAACGAGC CAAGAAAAAC CCAGAGGAGG ACTGTTCCAC TAAAACCCTG 
CCGTGGGAGC CGGGCCACCT GATGCTGACC CTCTACGTCA TCCGCAGCAT GGAGCAGCTG 
CTGCCCTTCT TCAACCTGCT CAGCCAGGTC TTCAACAGCA GGGCGAGCTG CCGATCGGGG 
CCCGTCTACA CCCACAATCC CGTGGATTCC TCCTTCCCCG CCAGCAAGGA GGGCCACAAG 
CTGGAGAGCC AGAAGGTGTT CTGGAGCCGA GCCCGACAGA ACATCGAGGA GATGGTGGAA 
AAAGACTTTC TAGAGGGTCT TATCAAGACA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOPEY1 ( XM_023330623.1 ) cDNA ORF clone, Xiphophorus maculatus(Southern platyfish) -> XP_023186391.1 Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X3, mRNA.

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CloneID	OXi35840
Clone ID Related Accession (Same CDS sequence)	XM_023330623.1
Accession Version	XM_023330623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	protein dopey-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036445.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAG AGGAAGTGGA GCTGCTCAGC GACTCAAAGT ACAGGAACTA TGTGGCAGCA 
GTGGACAAAG CCCTCAAGAA CTTTGAGTAT TCCAGCGAGT GGGCCGACCT CATCTCCGCA 
CTGGGCAAGC TCAACAAGGT TTTGCAGAAC AATGCAAAAT ATCAAGTTGT CCCCAAGAAG 
CTGACAATAG GCAAACGGCT GGCCCAGTGC CTCCACCCAG CCCTGCCCAG CGGCGTGCAC 
CGGAAGGCCT TGGAGACCTA CGAGATCATT TTCAAGATCA TTGGGCCCAA GAGGCTGGCC 
AAAGACCTCT TTCTGTACAG CTCGGGACTC TTCCCACTAC TCTCCAACGC TGCTATGTCT 
GTGAAGCCCG TGTTGCTGGG GCTCTATGAG ACCTACTACC TGCCCCTGGG GAAGACTCTC 
AAACCAGGCC TGCAGGGCTT ACTGACCGGC GTGCTGCCTG GTCTGGAGGA AGGTTCAGAG 
TATTACGACA GAACCAACAC GCTGTTGGAG AAAGTTGCTG CAGCCGTGGA GCAGTCGGCC 
TTCTACAGTG CCTTGTGGGG CAGCATCCTG ACCAGCCCCT CTGTGCGTCT CCCCGGGGTC 
TCCTTCGTTC TGCTGCACCT CAACCGGAAG CTCTCCATGG AGGACCAGCT CTACGTCATG 
GGCAGCGACA TTGAGCTTAT GGTGGAGGCT GTTAGTACCT CGGTGCAGGA CTCAAGCGTT 
TTGGTCCAGA GAAGCACTCT GGACCTCATC CTCTTCTGCT TCCCCTTCCA TATGAGCCAG 
GCTACCCGCC CTGACATGAT CCGGATCCTT TCAGCAGCTT TGCATGTGGT TCTGAGAAGA 
GACATGTCTC TGAACCGCAG ACTCTACGCC TGGCTGCTGG GCTTTGACAA TAATGGCGGG 
ATGTCAGGCC CCCGCAGTAC TCGGCAGAGC AACCCGGAGG AACACGCTTC CCACTACTTC 
AACAGCTTCT CCAAGGACAT GCTCGTCCAG GCAATGGTTG GGATTCTGCA GGGCAAGGCT 
CGAGGTGGGG AGGAGGAGAG CATCCTGATG CACGACCTCA AGCCATTTCG CATCCTGATC 
AGTCTGCTGG ACAAACCAGA ACTAGGGCCG GCCATTCTAG AGGACGTTCT GATCGAAGTG 
TTTCGGACTC TTTACACACA GTGCCGGACA GAGTTGGACC TTCAAAACCA GAGTCCGTTC 
AGCAAAGATC ACACACACCT CAGCAGTAAA CTGCGAGAAA ACAAGAAGAC GGCAGAGCTT 
ATCAAAACAG CCAATCTCCT GTTCAACTCG TTTGAGCCGT ACTACATGTG GGACTACATC 
GCTCGCTGGT TTGAGGAGTG CTGCAGGAGA ACATTGAACC GCAGCACACG TGTTGCACGA 
TACGGTTGGA GTCTGGATTC TCCTGATCTT TCTCTGGTGG AGTTCTGTCA GTTGGTAGAT 
TTTCTACTAG ACATTGTTTC ATTGCCTACT AGAAGCATGA GGGTAATCTG CCAGGAGACG 
TACATAGAAA TCCAGACGGA GCATCTCCCT CAACTGCTGT TGCGGATGGT GGCAGCTCTG 
ACCTGTCATC TCCAGGCGCT GGACCTCGGG GAGCTCACCC ACTGCCTTCG CCTCTGCTCC 
AAGATCCTCA GCAAGGTGCA GCCTCCGTTG GTGTCCCCGC TCGCCCTCCC CGTGAGTCCA 
CAGCCGCCGG GCCTGGCGGC ACAGAACAAA AGCAGAGATG TGGATGTTAA ACAGACTCCT 
CCCTCTGTCC CAGAGATGAC CGACAGTGGG GACGTGTTTG ACGATGGGGA AATCCCTACT 
GGCAGTCGCT CTGCAGAAAG TGGCTTCACA GACTTTGTTC AATACCGAGA AGATGGTTCC 
GAGGATCCAG AGCGAGCCTC TCATCCCCAC CCGCCTCTTA GAGCAGGCAG TCGTCCGTCA 
GGCTCGTGTC AGTCCAAGCC TTTGGACAAA CCAGTCATGC AGTGTTGCCT GGAGCACTTC 
CAGCAGTTTC TCTCCTGTCT GATCACTTTT TATATTGTTC CTGAGCACCT GGACAAAGCT 
GAGGGTCAGA GAGGTGAGCT CTTGCATTCG GGGCCTTTGG TTTCCGCAGG TTCCCAGCCC 
AGTAATTATA CGGAGCCGTG CTCAGGAGCT GGAGTGGTCC AGAAAGATTG TCTGGCTGCA 
TTTACTGCTG CCTGTCAGCT CTTCTTAGAA TGCTCCAGTT TCCCAGTCTA CATTGCAGAG 
GGCAACCTCA AGTCCTCGCC TGCACAGGAA GAGCAATGCG ACAGTGAGCA GGTTCGGCTG 
CCAGTGTGGC TGCAGACCCT GATGGATGCC TGCTGCCTGG CTGGTGACTT CAGCCTACAG 
GGTGTTGCCA TCTCCCTGCT TATGGACCTA ATAGGCCTGA CTCAGTCAGT TGCTGTGGTG 
ACGGCAGAGA GTCTGGCTTC CAGTAGCAGT ACGGAGCCCA CTCAGCCAAT GAGCCCCAGC 
CAGGGTCGGG TGGCTGTGGT CATTAGGCCT CCACTCACTC AGGGAATCCT GAAGTATATT 
GCGGATAAAA CGGACTTCTT CAAGAGCGTT GCAGTGATAT TATGGGACCA GCTGGGTGAA 
GGAACTCCTC AGCATCACCA GCGCAGTGTT GAGCTCTTTT ATCAGCTACA CAACCTGGTC 
CCTTCATCCA GCACCTGTGA GGACGTCATA AGCCAGCAAC TCATGCACAG AGACAAGAGA 
ATTCGTTTGG CAGCCCACGT GAAGTTCTCT GTTCTGTGGC ATTTAACGCG AGACTTGAAC 
ATCACCAAGT CTTCTCCGTT TAGTCGCACA TTTGACAGGT CTCTCTTCAT CATGCTCGAC 
AGCTTAAGTT ATTGGGATCC ATGTACCAGT GCGGTCGGTC GGGCTTGGTT AAATCAAGTC 
CTTCAGAGAC ATGACATTGC ACGGGTTCTT GAGCCACTCC TACTTCTTCT GCTCCATCCT 
AAAACTCACA GGGTTTCTAT TCAGAAGGTG CAAGCCCAAC GCCATTGGGC GCAGATATTT 
CCTGATCCAC CTGAGCAGGA ACCAACAGAA CCAATCTACA TTAGAGACTC CGGCTTCTCA 
GACAACTTTC TTCAGATTCG AAGCGAAGGG GGTGGGCATG AGGACTTCAG AGGTCTGACA 
ATAGGTGACA TGGAGCCATT TTGCCTGACG GTAAACCCCC TAAGTGACAG CTTGTCCATT 
CTTAGCCTGA GCAGTGAAAA CTTGCACTTA GCTGGTGAGT ATCAGACCAG GGACCAGCAG 
GAGGAGCAAC AGAGTTCAAA ATCAAGTGGT TCTCATTCAT CTACACTGGA AAACGGTAGC 
TTTGAAGACC CTGAGGGGGT CAGCTACATC GGAAATGGCT CAGATCGACC GCTTGGTTCA 
TCTGATGAAA CGCCTGACGA CTCCATAGAG GAAGCTGTGT CTTGTGTAGT GAAGGACCTG 
ATTGATAGGG TTCTGAATTT AGTTGATGAG GAATCAAATG AAACCTCATC TCCACCTGAA 
AATTGGCCCC AAACTGATAC TGAAAGCACT TCTTCAGACA CCTCCACCGG GATCCGCCTG 
GACTCCTGTC CTCCTCAATG CTCCAACCAC CAGAATCTGC CACAGTTATT GGCTGAAGGC 
ACACTAGAGT TCCTCTCTGT TACTCCAGCG GAGGGGCTAC ACAAAGAGGG CATCGCCCGC 
CACAGTTCAT CGCCCTCCAT CGTCATGTTG CCAGACAGCA CTGATGCAGC CACTCCAGAC 
CGCAGCCTGC AGGTGGAGGA CCCTCAGGCC CGCAAACGTA GCCACAGCAG CACTCAGCTT 
AGCCTTAAAG GGAAGATCAT GGAGAAGCTG GTAGACAAGT CTCCAGGGGC AAAGCCCAAG 
ATCAAGAAGA CTAAGAGGAA AGAGGAAGAG AAGCAGAAAA AAACGAACCA AGCTGAGAAA 
CTGCATCCAC TCAGTATTTT CTTTGGAGAC AGTCTAGACC TCGAGAACTG GTACAGCTGT 
GGTGAAGGGG AGGTGTCGGA GATCGAGAGT GACATGGGCT CACCGAGCGG GGGCTCTTGC 
GGGACCGGTG CTGGGGCGAA CGTCACAGGG CGCAGATCGT CCTCCACTCT GCCTCGTTTT 
AACATCCATC CTCTCTACCA GCACGTCCTG CTCTACCTAC AGCTCTACGA CTCCTCTAGG 
ACCCTGCATG CCCTGTCGGC GATCGCAGCC ATGGTGAGAG CCGCTCCCTC AGGCTTTGTA 
AGCGCCATCT CCACCACCAG CATCAATAAC ACCTACACTC CACAGCTCTC CTTGCTGCAA 
AACCTACTTG CTCGCCACAG AGTCTCTGTT ATGGGCAAAG ACTTCTACTG CCCCATTCCC 
CAGGACTCTC ACTCCCATTC TTTCCGCAGT GCCATGTACC TGGAGATCAT CATCTCCCTC 
TGTCTCTACT TCCTGAGAAG CTATTACTCT GCCCATGTGA CATTGGGTCC TCAGGACTTG 
GCAGGAAACC GGGCCATGCA GCTGACCAGC GTGGAGGTCT TGACACTGCT ATTCAGCGAA 
CTAGCCAAAG TCACAGCTGG CTCGGCAAAG GGTTTTGCTA GCTTCATCAG TGACGTCCTT 
TCCAAGTGCA AAGTTCAGAA AGTCATCCTC CATTGCCTGC TTTCCTCCAT ATTCAGCGCC 
CAGAAATGGC ATGACCAACG GACGGCAGGG GTCAACGTGG CCACGGTGGA GGAAGGTCTC 
TCGGAGGACA GCGTCATCAA CATGTCGGAG GACCAGATAG ACAGCTGCAG CGCCGTTCAG 
TCCCAGCTGC TCCGGCTGCT GCAGAGTCTA GTTGTACTTG AGCATAGGGT CTTAATGCCA 
GCAGACGAAG GTGGAGAAGG AGGACCTGCC GCAGGAGGAG CAGGTGGAGG GCCAGGAAGC 
GGTGGAGTGG CAGGATTTGA GATCCTAGGA GGGGAGGTGG AGCATGTTAA CCCTCAGCAG 
CCTATGACGT CACTGCAGTA CCTCCATGGT CAGCCTATTA CAGCTCAGGG CATGTTCCTG 
TGTGCAGTCA TCAGGGCCCT GCACCAGCAC CATGCCTGTA AGATGCACCC CCAATGGATA 
GGGCTGATCA CAACCACCCT GCCTTACATG GGGAGGGTCC TGAGGAGGGT GGTGGCCTCA 
GTGACTCTGC AGCTATGCAG AAACCTGGAT AATCTCCTCC AGCAGTACCG CTATGAAACC 
AGCATAACTG ACACCAGACC CCAGTGGATG GCTCTCTGCA TCCCTCCAGA CCTGATTCTG 
ACTGTGCTGG AGGGAATCAC TTCCATTATC CATTACTGTC TGCTGGACCC CACTTCACAG 
TACCACCAGT TACAAGTGAG TGTTGACCAG AAGCACCTGG CCGAGGCGCG TTCAGGTATC 
CTGTCCATCC TCCACACCAT CATGTCTTCC GTCACGCTGC TGTGGAGCGT GCTCCACCAA 
GCCGACAGCT CTGACAAGCC AGCTGCCGCT TCAGCTGCCG CCACCTCAAA CATCAACCTA 
GGCTCCACCA AGAATCTCCG TCAGCAGATC TTGGAACTGC TGGGTCCAAT CTCCATGAAC 
CACGGCGCTC ATTTCATGGC TGCAATTGCT TATGTTTGGA ACGAGAGAAA ACAAGTCAAG 
GCGCCAATCA GAAATAAGGT CATCCCTGTA GCGAGTGAGG AGCAGCTGCT GCTGGTTGAG 
CTCGTTCGGT CGGTCAGCGC CATGCGCACT GAGACTGTTA TGCAGACAGT GAAGGAGGTC 
CTGAAGCAGC CACCTGCTAT CGCAAAAGAC AAGAAGCACA TCTCTTTGGA AGTCTGCATG 
CTACAGTTCT TCTATGCCTA TGTACAGAGG ATTCCTGTTT CCAGTTTGCT TGATAGCTGG 
CCGTCTCTGC TGGCGCTGCT GAAGGACTCG GTCCAGTTGG GTCTGCCTGC TCCTGGGCAG 
TTTCTCATAC TGGGAGTTCT GAATGAGTTT ATCTTGAAGA ATCCCAACCT GGAGAGTAAG 
AAAGACCAGC GAGAGCTGCA GGATGTGACT CACAAGGTGG TGGAGGCCAT CGGCACCATC 
GCTGGCTCCT CTTTGGAACA AACCACGTGG CTAAGGAGGA ACCTGGAGGT GAAGGCATCG 
CCTCAGATTG TTGTGGACGG AACCAACCTG GAGGCAGACG TAGAAGACTT AATGCTCACA 
GTGATGGAGG CCTCCAGCTT CACTCCATCT GTGTACAGTG TTCACGCCCT CACGCTGCTG 
GCCGAGGTGC TGGCTCATCT ATTGGACATG GTGTTCTACA GTGACGAGAA GGAGAAAGTC 
ATCCCTCTGC TGGTTAACAT TATGCACTAC GTAGTTCCCT ACCTCCGCAA CCACAGTGCT 
CACAATGCCC CCAGCTACCG AGCCTGCATC CAGCTGCTGA GCAGCCTGAG CGGCTACCAG 
TACACCCGCC GTGCCTGGAA AAAAGAGGCC TTCGACCTCT TCATGGACCA CACCTTCTTC 
CAGATGGATG CTTCGTGTGT CAGCCACTGG AGAGCCATCA TTGACCACCT GATGACTCAT 
GACAAGACCA CATTTAGAGA CCTGATGACC CGGGTGGCGG TGGCTCAGAG CAGCTCCCTG 
AGTTTGTTCA CCAACAGAGA CGCTGAGCTG GAGCAGAGGG CCATGCTGCT CAAACGCCTG 
GCCTTCACCA TCTACAGCAG CGAGGTGGAC CAGTACCAGA AGTACCTGCC GGACATCCAA 
GAGCGTCTGG TGGAGAGCCT GCGTCTGCCT CAGGTACCCA TCCTCCACGC TCAGGTGTTC 
CTGTTCTTCA GAGTGCTGCT GCTGCGCATG TCGCCTCAAC ACCTCACGTC ACTCTGGCCC 
ACCATGATCA CTGAGCTGGT CCAAGTGTTT CTGCTGATGG AGCAGGAGTT AACCGCTGAC 
GAGGACATAT CAAGGACGTC AGGACCGTCG GTTGTCGGGT TGGAGACCAC CTACTCTGGA 
GGGAACGGCT TCTCCACGTC CTACAACAGC CAGCGCTGGC TCAACCTCTA CCTCTCTGCT 
TGCAAGTTCC TGGATCTTGC CTTGGCGTTG CCTCCTGAGA GTCTCCCTCA GTTCCAGATG 
TACCGCTGGG CCTTCATCCC AGAGGCTTCA GACGATTCAG GGTTGGAAGT GAGACGCCAG 
GGGACGCATC AGAGGGAGTT CAAGCCCTAC GTGGTCCGTC TGGTGAAGCT GCTGAGAAAA 
CGAGCCAAGA AAAACCCAGA GGAGGACTGT TCCACTAAAA CCCTGCCGTG GGAGCCGGGC 
CACCTGATGC TGACCCTCTA CGTCATCCGC AGCATGGAGC AGCTGCTGCC CTTCTTCAAC 
CTGCTCAGCC AGGTCTTCAA CAGCAGGGCG AGCTGCCGAT CGGGGCCCGT CTACACCCAC 
AATCCCGTGG ATTCCTCCTT CCCCGCCAGC AAGGAGGGCC ACAAGCTGGA GAGCCAGAAG 
GTGTTCTGGA GCCGAGCCCG ACAGAACATC GAGGAGATGG TGGAAAAAGA CTTTCTAGAG 
GGTCTTATCA AGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023186391.1
CDS	395..7852
Translation	MNAEEVELLSDSKYRNYVAAVDKALKNFEYSSEWADLISALGKLNKVLQNNAKYQVVPKKLTIGKRLAQCLHPALPSGVHRKALETYEIIFKIIGPKRLAKDLFLYSSGLFPLLSNAAMSVKPVLLGLYETYYLPLGKTLKPGLQGLLTGVLPGLEEGSEYYDRTNTLLEKVAAAVEQSAFYSALWGSILTSPSVRLPGVSFVLLHLNRKLSMEDQLYVMGSDIELMVEAVSTSVQDSSVLVQRSTLDLILFCFPFHMSQATRPDMIRILSAALHVVLRRDMSLNRRLYAWLLGFDNNGGMSGPRSTRQSNPEEHASHYFNSFSKDMLVQAMVGILQGKARGGEEESILMHDLKPFRILISLLDKPELGPAILEDVLIEVFRTLYTQCRTELDLQNQSPFSKDHTHLSSKLRENKKTAELIKTANLLFNSFEPYYMWDYIARWFEECCRRTLNRSTRVARYGWSLDSPDLSLVEFCQLVDFLLDIVSLPTRSMRVICQETYIEIQTEHLPQLLLRMVAALTCHLQALDLGELTHCLRLCSKILSKVQPPLVSPLALPVSPQPPGLAAQNKSRDVDVKQTPPSVPEMTDSGDVFDDGEIPTGSRSAESGFTDFVQYREDGSEDPERASHPHPPLRAGSRPSGSCQSKPLDKPVMQCCLEHFQQFLSCLITFYIVPEHLDKAEGQRGELLHSGPLVSAGSQPSNYTEPCSGAGVVQKDCLAAFTAACQLFLECSSFPVYIAEGNLKSSPAQEEQCDSEQVRLPVWLQTLMDACCLAGDFSLQGVAISLLMDLIGLTQSVAVVTAESLASSSSTEPTQPMSPSQGRVAVVIRPPLTQGILKYIADKTDFFKSVAVILWDQLGEGTPQHHQRSVELFYQLHNLVPSSSTCEDVISQQLMHRDKRIRLAAHVKFSVLWHLTRDLNITKSSPFSRTFDRSLFIMLDSLSYWDPCTSAVGRAWLNQVLQRHDIARVLEPLLLLLLHPKTHRVSIQKVQAQRHWAQIFPDPPEQEPTEPIYIRDSGFSDNFLQIRSEGGGHEDFRGLTIGDMEPFCLTVNPLSDSLSILSLSSENLHLAGEYQTRDQQEEQQSSKSSGSHSSTLENGSFEDPEGVSYIGNGSDRPLGSSDETPDDSIEEAVSCVVKDLIDRVLNLVDEESNETSSPPENWPQTDTESTSSDTSTGIRLDSCPPQCSNHQNLPQLLAEGTLEFLSVTPAEGLHKEGIARHSSSPSIVMLPDSTDAATPDRSLQVEDPQARKRSHSSTQLSLKGKIMEKLVDKSPGAKPKIKKTKRKEEEKQKKTNQAEKLHPLSIFFGDSLDLENWYSCGEGEVSEIESDMGSPSGGSCGTGAGANVTGRRSSSTLPRFNIHPLYQHVLLYLQLYDSSRTLHALSAIAAMVRAAPSGFVSAISTTSINNTYTPQLSLLQNLLARHRVSVMGKDFYCPIPQDSHSHSFRSAMYLEIIISLCLYFLRSYYSAHVTLGPQDLAGNRAMQLTSVEVLTLLFSELAKVTAGSAKGFASFISDVLSKCKVQKVILHCLLSSIFSAQKWHDQRTAGVNVATVEEGLSEDSVINMSEDQIDSCSAVQSQLLRLLQSLVVLEHRVLMPADEGGEGGPAAGGAGGGPGSGGVAGFEILGGEVEHVNPQQPMTSLQYLHGQPITAQGMFLCAVIRALHQHHACKMHPQWIGLITTTLPYMGRVLRRVVASVTLQLCRNLDNLLQQYRYETSITDTRPQWMALCIPPDLILTVLEGITSIIHYCLLDPTSQYHQLQVSVDQKHLAEARSGILSILHTIMSSVTLLWSVLHQADSSDKPAAASAAATSNINLGSTKNLRQQILELLGPISMNHGAHFMAAIAYVWNERKQVKAPIRNKVIPVASEEQLLLVELVRSVSAMRTETVMQTVKEVLKQPPAIAKDKKHISLEVCMLQFFYAYVQRIPVSSLLDSWPSLLALLKDSVQLGLPAPGQFLILGVLNEFILKNPNLESKKDQRELQDVTHKVVEAIGTIAGSSLEQTTWLRRNLEVKASPQIVVDGTNLEADVEDLMLTVMEASSFTPSVYSVHALTLLAEVLAHLLDMVFYSDEKEKVIPLLVNIMHYVVPYLRNHSAHNAPSYRACIQLLSSLSGYQYTRRAWKKEAFDLFMDHTFFQMDASCVSHWRAIIDHLMTHDKTTFRDLMTRVAVAQSSSLSLFTNRDAELEQRAMLLKRLAFTIYSSEVDQYQKYLPDIQERLVESLRLPQVPILHAQVFLFFRVLLLRMSPQHLTSLWPTMITELVQVFLLMEQELTADEDISRTSGPSVVGLETTYSGGNGFSTSYNSQRWLNLYLSACKFLDLALALPPESLPQFQMYRWAFIPEASDDSGLEVRRQGTHQREFKPYVVRLVKLLRKRAKKNPEEDCSTKTLPWEPGHLMLTLYVIRSMEQLLPFFNLLSQVFNSRASCRSGPVYTHNPVDSSFPASKEGHKLESQKVFWSRARQNIEEMVEKDFLEGLIKT

Target ORF information:

RefSeq Version	XM_023330623.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023330623.1

ORF Insert Sequence:

ATGAATGCAG AGGAAGTGGA GCTGCTCAGC GACTCAAAGT ACAGGAACTA TGTGGCAGCA 
GTGGACAAAG CCCTCAAGAA CTTTGAGTAT TCCAGCGAGT GGGCCGACCT CATCTCCGCA 
CTGGGCAAGC TCAACAAGGT TTTGCAGAAC AATGCAAAAT ATCAAGTTGT CCCCAAGAAG 
CTGACAATAG GCAAACGGCT GGCCCAGTGC CTCCACCCAG CCCTGCCCAG CGGCGTGCAC 
CGGAAGGCCT TGGAGACCTA CGAGATCATT TTCAAGATCA TTGGGCCCAA GAGGCTGGCC 
AAAGACCTCT TTCTGTACAG CTCGGGACTC TTCCCACTAC TCTCCAACGC TGCTATGTCT 
GTGAAGCCCG TGTTGCTGGG GCTCTATGAG ACCTACTACC TGCCCCTGGG GAAGACTCTC 
AAACCAGGCC TGCAGGGCTT ACTGACCGGC GTGCTGCCTG GTCTGGAGGA AGGTTCAGAG 
TATTACGACA GAACCAACAC GCTGTTGGAG AAAGTTGCTG CAGCCGTGGA GCAGTCGGCC 
TTCTACAGTG CCTTGTGGGG CAGCATCCTG ACCAGCCCCT CTGTGCGTCT CCCCGGGGTC 
TCCTTCGTTC TGCTGCACCT CAACCGGAAG CTCTCCATGG AGGACCAGCT CTACGTCATG 
GGCAGCGACA TTGAGCTTAT GGTGGAGGCT GTTAGTACCT CGGTGCAGGA CTCAAGCGTT 
TTGGTCCAGA GAAGCACTCT GGACCTCATC CTCTTCTGCT TCCCCTTCCA TATGAGCCAG 
GCTACCCGCC CTGACATGAT CCGGATCCTT TCAGCAGCTT TGCATGTGGT TCTGAGAAGA 
GACATGTCTC TGAACCGCAG ACTCTACGCC TGGCTGCTGG GCTTTGACAA TAATGGCGGG 
ATGTCAGGCC CCCGCAGTAC TCGGCAGAGC AACCCGGAGG AACACGCTTC CCACTACTTC 
AACAGCTTCT CCAAGGACAT GCTCGTCCAG GCAATGGTTG GGATTCTGCA GGGCAAGGCT 
CGAGGTGGGG AGGAGGAGAG CATCCTGATG CACGACCTCA AGCCATTTCG CATCCTGATC 
AGTCTGCTGG ACAAACCAGA ACTAGGGCCG GCCATTCTAG AGGACGTTCT GATCGAAGTG 
TTTCGGACTC TTTACACACA GTGCCGGACA GAGTTGGACC TTCAAAACCA GAGTCCGTTC 
AGCAAAGATC ACACACACCT CAGCAGTAAA CTGCGAGAAA ACAAGAAGAC GGCAGAGCTT 
ATCAAAACAG CCAATCTCCT GTTCAACTCG TTTGAGCCGT ACTACATGTG GGACTACATC 
GCTCGCTGGT TTGAGGAGTG CTGCAGGAGA ACATTGAACC GCAGCACACG TGTTGCACGA 
TACGGTTGGA GTCTGGATTC TCCTGATCTT TCTCTGGTGG AGTTCTGTCA GTTGGTAGAT 
TTTCTACTAG ACATTGTTTC ATTGCCTACT AGAAGCATGA GGGTAATCTG CCAGGAGACG 
TACATAGAAA TCCAGACGGA GCATCTCCCT CAACTGCTGT TGCGGATGGT GGCAGCTCTG 
ACCTGTCATC TCCAGGCGCT GGACCTCGGG GAGCTCACCC ACTGCCTTCG CCTCTGCTCC 
AAGATCCTCA GCAAGGTGCA GCCTCCGTTG GTGTCCCCGC TCGCCCTCCC CGTGAGTCCA 
CAGCCGCCGG GCCTGGCGGC ACAGAACAAA AGCAGAGATG TGGATGTTAA ACAGACTCCT 
CCCTCTGTCC CAGAGATGAC CGACAGTGGG GACGTGTTTG ACGATGGGGA AATCCCTACT 
GGCAGTCGCT CTGCAGAAAG TGGCTTCACA GACTTTGTTC AATACCGAGA AGATGGTTCC 
GAGGATCCAG AGCGAGCCTC TCATCCCCAC CCGCCTCTTA GAGCAGGCAG TCGTCCGTCA 
GGCTCGTGTC AGTCCAAGCC TTTGGACAAA CCAGTCATGC AGTGTTGCCT GGAGCACTTC 
CAGCAGTTTC TCTCCTGTCT GATCACTTTT TATATTGTTC CTGAGCACCT GGACAAAGCT 
GAGGGTCAGA GAGGTGAGCT CTTGCATTCG GGGCCTTTGG TTTCCGCAGG TTCCCAGCCC 
AGTAATTATA CGGAGCCGTG CTCAGGAGCT GGAGTGGTCC AGAAAGATTG TCTGGCTGCA 
TTTACTGCTG CCTGTCAGCT CTTCTTAGAA TGCTCCAGTT TCCCAGTCTA CATTGCAGAG 
GGCAACCTCA AGTCCTCGCC TGCACAGGAA GAGCAATGCG ACAGTGAGCA GGTTCGGCTG 
CCAGTGTGGC TGCAGACCCT GATGGATGCC TGCTGCCTGG CTGGTGACTT CAGCCTACAG 
GGTGTTGCCA TCTCCCTGCT TATGGACCTA ATAGGCCTGA CTCAGTCAGT TGCTGTGGTG 
ACGGCAGAGA GTCTGGCTTC CAGTAGCAGT ACGGAGCCCA CTCAGCCAAT GAGCCCCAGC 
CAGGGTCGGG TGGCTGTGGT CATTAGGCCT CCACTCACTC AGGGAATCCT GAAGTATATT 
GCGGATAAAA CGGACTTCTT CAAGAGCGTT GCAGTGATAT TATGGGACCA GCTGGGTGAA 
GGAACTCCTC AGCATCACCA GCGCAGTGTT GAGCTCTTTT ATCAGCTACA CAACCTGGTC 
CCTTCATCCA GCACCTGTGA GGACGTCATA AGCCAGCAAC TCATGCACAG AGACAAGAGA 
ATTCGTTTGG CAGCCCACGT GAAGTTCTCT GTTCTGTGGC ATTTAACGCG AGACTTGAAC 
ATCACCAAGT CTTCTCCGTT TAGTCGCACA TTTGACAGGT CTCTCTTCAT CATGCTCGAC 
AGCTTAAGTT ATTGGGATCC ATGTACCAGT GCGGTCGGTC GGGCTTGGTT AAATCAAGTC 
CTTCAGAGAC ATGACATTGC ACGGGTTCTT GAGCCACTCC TACTTCTTCT GCTCCATCCT 
AAAACTCACA GGGTTTCTAT TCAGAAGGTG CAAGCCCAAC GCCATTGGGC GCAGATATTT 
CCTGATCCAC CTGAGCAGGA ACCAACAGAA CCAATCTACA TTAGAGACTC CGGCTTCTCA 
GACAACTTTC TTCAGATTCG AAGCGAAGGG GGTGGGCATG AGGACTTCAG AGGTCTGACA 
ATAGGTGACA TGGAGCCATT TTGCCTGACG GTAAACCCCC TAAGTGACAG CTTGTCCATT 
CTTAGCCTGA GCAGTGAAAA CTTGCACTTA GCTGGTGAGT ATCAGACCAG GGACCAGCAG 
GAGGAGCAAC AGAGTTCAAA ATCAAGTGGT TCTCATTCAT CTACACTGGA AAACGGTAGC 
TTTGAAGACC CTGAGGGGGT CAGCTACATC GGAAATGGCT CAGATCGACC GCTTGGTTCA 
TCTGATGAAA CGCCTGACGA CTCCATAGAG GAAGCTGTGT CTTGTGTAGT GAAGGACCTG 
ATTGATAGGG TTCTGAATTT AGTTGATGAG GAATCAAATG AAACCTCATC TCCACCTGAA 
AATTGGCCCC AAACTGATAC TGAAAGCACT TCTTCAGACA CCTCCACCGG GATCCGCCTG 
GACTCCTGTC CTCCTCAATG CTCCAACCAC CAGAATCTGC CACAGTTATT GGCTGAAGGC 
ACACTAGAGT TCCTCTCTGT TACTCCAGCG GAGGGGCTAC ACAAAGAGGG CATCGCCCGC 
CACAGTTCAT CGCCCTCCAT CGTCATGTTG CCAGACAGCA CTGATGCAGC CACTCCAGAC 
CGCAGCCTGC AGGTGGAGGA CCCTCAGGCC CGCAAACGTA GCCACAGCAG CACTCAGCTT 
AGCCTTAAAG GGAAGATCAT GGAGAAGCTG GTAGACAAGT CTCCAGGGGC AAAGCCCAAG 
ATCAAGAAGA CTAAGAGGAA AGAGGAAGAG AAGCAGAAAA AAACGAACCA AGCTGAGAAA 
CTGCATCCAC TCAGTATTTT CTTTGGAGAC AGTCTAGACC TCGAGAACTG GTACAGCTGT 
GGTGAAGGGG AGGTGTCGGA GATCGAGAGT GACATGGGCT CACCGAGCGG GGGCTCTTGC 
GGGACCGGTG CTGGGGCGAA CGTCACAGGG CGCAGATCGT CCTCCACTCT GCCTCGTTTT 
AACATCCATC CTCTCTACCA GCACGTCCTG CTCTACCTAC AGCTCTACGA CTCCTCTAGG 
ACCCTGCATG CCCTGTCGGC GATCGCAGCC ATGGTGAGAG CCGCTCCCTC AGGCTTTGTA 
AGCGCCATCT CCACCACCAG CATCAATAAC ACCTACACTC CACAGCTCTC CTTGCTGCAA 
AACCTACTTG CTCGCCACAG AGTCTCTGTT ATGGGCAAAG ACTTCTACTG CCCCATTCCC 
CAGGACTCTC ACTCCCATTC TTTCCGCAGT GCCATGTACC TGGAGATCAT CATCTCCCTC 
TGTCTCTACT TCCTGAGAAG CTATTACTCT GCCCATGTGA CATTGGGTCC TCAGGACTTG 
GCAGGAAACC GGGCCATGCA GCTGACCAGC GTGGAGGTCT TGACACTGCT ATTCAGCGAA 
CTAGCCAAAG TCACAGCTGG CTCGGCAAAG GGTTTTGCTA GCTTCATCAG TGACGTCCTT 
TCCAAGTGCA AAGTTCAGAA AGTCATCCTC CATTGCCTGC TTTCCTCCAT ATTCAGCGCC 
CAGAAATGGC ATGACCAACG GACGGCAGGG GTCAACGTGG CCACGGTGGA GGAAGGTCTC 
TCGGAGGACA GCGTCATCAA CATGTCGGAG GACCAGATAG ACAGCTGCAG CGCCGTTCAG 
TCCCAGCTGC TCCGGCTGCT GCAGAGTCTA GTTGTACTTG AGCATAGGGT CTTAATGCCA 
GCAGACGAAG GTGGAGAAGG AGGACCTGCC GCAGGAGGAG CAGGTGGAGG GCCAGGAAGC 
GGTGGAGTGG CAGGATTTGA GATCCTAGGA GGGGAGGTGG AGCATGTTAA CCCTCAGCAG 
CCTATGACGT CACTGCAGTA CCTCCATGGT CAGCCTATTA CAGCTCAGGG CATGTTCCTG 
TGTGCAGTCA TCAGGGCCCT GCACCAGCAC CATGCCTGTA AGATGCACCC CCAATGGATA 
GGGCTGATCA CAACCACCCT GCCTTACATG GGGAGGGTCC TGAGGAGGGT GGTGGCCTCA 
GTGACTCTGC AGCTATGCAG AAACCTGGAT AATCTCCTCC AGCAGTACCG CTATGAAACC 
AGCATAACTG ACACCAGACC CCAGTGGATG GCTCTCTGCA TCCCTCCAGA CCTGATTCTG 
ACTGTGCTGG AGGGAATCAC TTCCATTATC CATTACTGTC TGCTGGACCC CACTTCACAG 
TACCACCAGT TACAAGTGAG TGTTGACCAG AAGCACCTGG CCGAGGCGCG TTCAGGTATC 
CTGTCCATCC TCCACACCAT CATGTCTTCC GTCACGCTGC TGTGGAGCGT GCTCCACCAA 
GCCGACAGCT CTGACAAGCC AGCTGCCGCT TCAGCTGCCG CCACCTCAAA CATCAACCTA 
GGCTCCACCA AGAATCTCCG TCAGCAGATC TTGGAACTGC TGGGTCCAAT CTCCATGAAC 
CACGGCGCTC ATTTCATGGC TGCAATTGCT TATGTTTGGA ACGAGAGAAA ACAAGTCAAG 
GCGCCAATCA GAAATAAGGT CATCCCTGTA GCGAGTGAGG AGCAGCTGCT GCTGGTTGAG 
CTCGTTCGGT CGGTCAGCGC CATGCGCACT GAGACTGTTA TGCAGACAGT GAAGGAGGTC 
CTGAAGCAGC CACCTGCTAT CGCAAAAGAC AAGAAGCACA TCTCTTTGGA AGTCTGCATG 
CTACAGTTCT TCTATGCCTA TGTACAGAGG ATTCCTGTTT CCAGTTTGCT TGATAGCTGG 
CCGTCTCTGC TGGCGCTGCT GAAGGACTCG GTCCAGTTGG GTCTGCCTGC TCCTGGGCAG 
TTTCTCATAC TGGGAGTTCT GAATGAGTTT ATCTTGAAGA ATCCCAACCT GGAGAGTAAG 
AAAGACCAGC GAGAGCTGCA GGATGTGACT CACAAGGTGG TGGAGGCCAT CGGCACCATC 
GCTGGCTCCT CTTTGGAACA AACCACGTGG CTAAGGAGGA ACCTGGAGGT GAAGGCATCG 
CCTCAGATTG TTGTGGACGG AACCAACCTG GAGGCAGACG TAGAAGACTT AATGCTCACA 
GTGATGGAGG CCTCCAGCTT CACTCCATCT GTGTACAGTG TTCACGCCCT CACGCTGCTG 
GCCGAGGTGC TGGCTCATCT ATTGGACATG GTGTTCTACA GTGACGAGAA GGAGAAAGTC 
ATCCCTCTGC TGGTTAACAT TATGCACTAC GTAGTTCCCT ACCTCCGCAA CCACAGTGCT 
CACAATGCCC CCAGCTACCG AGCCTGCATC CAGCTGCTGA GCAGCCTGAG CGGCTACCAG 
TACACCCGCC GTGCCTGGAA AAAAGAGGCC TTCGACCTCT TCATGGACCA CACCTTCTTC 
CAGATGGATG CTTCGTGTGT CAGCCACTGG AGAGCCATCA TTGACCACCT GATGACTCAT 
GACAAGACCA CATTTAGAGA CCTGATGACC CGGGTGGCGG TGGCTCAGAG CAGCTCCCTG 
AGTTTGTTCA CCAACAGAGA CGCTGAGCTG GAGCAGAGGG CCATGCTGCT CAAACGCCTG 
GCCTTCACCA TCTACAGCAG CGAGGTGGAC CAGTACCAGA AGTACCTGCC GGACATCCAA 
GAGCGTCTGG TGGAGAGCCT GCGTCTGCCT CAGGTACCCA TCCTCCACGC TCAGGTGTTC 
CTGTTCTTCA GAGTGCTGCT GCTGCGCATG TCGCCTCAAC ACCTCACGTC ACTCTGGCCC 
ACCATGATCA CTGAGCTGGT CCAAGTGTTT CTGCTGATGG AGCAGGAGTT AACCGCTGAC 
GAGGACATAT CAAGGACGTC AGGACCGTCG GTTGTCGGGT TGGAGACCAC CTACTCTGGA 
GGGAACGGCT TCTCCACGTC CTACAACAGC CAGCGCTGGC TCAACCTCTA CCTCTCTGCT 
TGCAAGTTCC TGGATCTTGC CTTGGCGTTG CCTCCTGAGA GTCTCCCTCA GTTCCAGATG 
TACCGCTGGG CCTTCATCCC AGAGGCTTCA GACGATTCAG GGTTGGAAGT GAGACGCCAG 
GGGACGCATC AGAGGGAGTT CAAGCCCTAC GTGGTCCGTC TGGTGAAGCT GCTGAGAAAA 
CGAGCCAAGA AAAACCCAGA GGAGGACTGT TCCACTAAAA CCCTGCCGTG GGAGCCGGGC 
CACCTGATGC TGACCCTCTA CGTCATCCGC AGCATGGAGC AGCTGCTGCC CTTCTTCAAC 
CTGCTCAGCC AGGTCTTCAA CAGCAGGGCG AGCTGCCGAT CGGGGCCCGT CTACACCCAC 
AATCCCGTGG ATTCCTCCTT CCCCGCCAGC AAGGAGGGCC ACAAGCTGGA GAGCCAGAAG 
GTGTTCTGGA GCCGAGCCCG ACAGAACATC GAGGAGATGG TGGAAAAAGA CTTTCTAGAG 
GGTCTTATCA AGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOPEY1 ( XM_023330624.1 ) cDNA ORF clone, Xiphophorus maculatus(Southern platyfish) -> XP_023186392.1 Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X4, mRNA.

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CloneID	OXi35841
Clone ID Related Accession (Same CDS sequence)	XM_023330624.1
Accession Version	XM_023330624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	protein dopey-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036445.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAG AGGAAGTGGA GCTGCTCAGC GACTCAAAGT ACAGGAACTA TGTGGCAGCA 
GTGGACAAAG CCCTCAAGAA CTTTGAGTAT TCCAGCGAGT GGGCCGACCT CATCTCCGCA 
CTGGGCAAGC TCAACAAGGT TTTGCAGAAC AATGCAAAAT ATCAAGTTGT CCCCAAGAAG 
CTGACAATAG GCAAACGGCT GGCCCAGTGC CTCCACCCAG CCCTGCCCAG CGGCGTGCAC 
CGGAAGGCCT TGGAGACCTA CGAGATCATT TTCAAGATCA TTGGGCCCAA GAGGCTGGCC 
AAAGACCTCT TTCTGTACAG CTCGGGACTC TTCCCACTAC TCTCCAACGC TGCTATGTCT 
GTGAAGCCCG TGTTGCTGGG GCTCTATGAG ACCTACTACC TGCCCCTGGG GAAGACTCTC 
AAACCAGGCC TGCAGGGCTT ACTGACCGGC GTGCTGCCTG GTCTGGAGGA AGGTTCAGAG 
TATTACGACA GAACCAACAC GCTGTTGGAG AAAGTTGCTG CAGCCGTGGA GCAGTCGGCC 
TTCTACAGTG CCTTGTGGGG CAGCATCCTG ACCAGCCCCT CTGTGCGTCT CCCCGGGGTC 
TCCTTCGTTC TGCTGCACCT CAACCGGAAG CTCTCCATGG AGGACCAGCT CTACGTCATG 
GGCAGCGACA TTGAGCTTAT GGTGGAGGCT GTTAGTACCT CGGTGCAGGA CTCAAGCGTT 
TTGGTCCAGA GAAGCACTCT GGACCTCATC CTCTTCTGCT TCCCCTTCCA TATGAGCCAG 
GCTACCCGCC CTGACATGAT CCGGATCCTT TCAGCAGCTT TGCATGTGGT TCTGAGAAGA 
GACATGTCTC TGAACCGCAG ACTCTACGCC TGGCTGCTGG GCCCCCGCAG TACTCGGCAG 
AGCAACCCGG AGGAACACGC TTCCCACTAC TTCAACAGCT TCTCCAAGGA CATGCTCGTC 
CAGGCAATGG TTGGGATTCT GCAGGGCAAG GCTCGAGGTG GGGAGGAGGA GAGCATCCTG 
ATGCACGACC TCAAGCCATT TCGCATCCTG ATCAGTCTGC TGGACAAACC AGAACTAGGG 
CCGGCCATTC TAGAGGACGT TCTGATCGAA GTGTTTCGGA CTCTTTACAC ACAGTGCCGG 
ACAGAGTTGG ACCTTCAAAA CCAGAGTCCG TTCAGCAAAG ATCACACACA CCTCAGCAGT 
AAACTGCGAG AAAACAAGAA GACGGCAGAG CTTATCAAAA CAGCCAATCT CCTGTTCAAC 
TCGTTTGAGC CGTACTACAT GTGGGACTAC ATCGCTCGCT GGTTTGAGGA GTGCTGCAGG 
AGAACATTGA ACCGCAGCAC ACGTGTTGCA CGATACGGTT GGAGTCTGGA TTCTCCTGAT 
CTTTCTCTGG TGGAGTTCTG TCAGTTGGTA GATTTTCTAC TAGACATTGT TTCATTGCCT 
ACTAGAAGCA TGAGGGTAAT CTGCCAGGAG ACGTACATAG AAATCCAGAC GGAGCATCTC 
CCTCAACTGC TGTTGCGGAT GGTGGCAGCT CTGACCTGTC ATCTCCAGGC GCTGGACCTC 
GGGGAGCTCA CCCACTGCCT TCGCCTCTGC TCCAAGATCC TCAGCAAGGT GCAGCCTCCG 
TTGGTGTCCC CGCTCGCCCT CCCCGTGAGT CCACAGCCGC CGGGCCTGGC GGCACAGAAC 
AAAAGCAGAG ATGTGGATGT TAAACAGACT CCTCCCTCTG TCCCAGAGAT GACCGACAGT 
GGGGACGTGT TTGACGATGG GGAAATCCCT ACTGGCAGTC GCTCTGCAGA AAGTGGCTTC 
ACAGACTTTG TTCAATACCG AGAAGATGGT TCCGAGGATC CAGAGCGAGC CTCTCATCCC 
CACCCGCCTC TTAGAGCAGG CAGTCGTCCG TCAGGCTCGT GTCAGTCCAA GCCTTTGGAC 
AAACCAGTCA TGCAGTGTTG CCTGGAGCAC TTCCAGCAGT TTCTCTCCTG TCTGATCACT 
TTTTATATTG TTCCTGAGCA CCTGGACAAA GCTGAGGGTC AGAGAGGTGA GCTCTTGCAT 
TCGGGGCCTT TGGTTTCCGC AGGTTCCCAG CCCAGTAATT ATACGGAGCC GTGCTCAGGA 
GCTGGAGTGG TCCAGAAAGA TTGTCTGGCT GCATTTACTG CTGCCTGTCA GCTCTTCTTA 
GAATGCTCCA GTTTCCCAGT CTACATTGCA GAGGGCAACC TCAAGTCCTC GCCTGCACAG 
GAAGAGCAAT GCGACAGTGA GCAGGTTCGG CTGCCAGTGT GGCTGCAGAC CCTGATGGAT 
GCCTGCTGCC TGGCTGGTGA CTTCAGCCTA CAGGGTGTTG CCATCTCCCT GCTTATGGAC 
CTAATAGGCC TGACTCAGTC AGTTGCTGTG GTGACGGCAG AGAGTCTGGC TTCCAGTAGC 
AGTACGGAGC CCACTCAGCC AATGAGCCCC AGCCAGGGTC GGGTGGCTGT GGTCATTAGG 
CCTCCACTCA CTCAGGGAAT CCTGAAGTAT ATTGCGGATA AAACGGACTT CTTCAAGAGC 
GTTGCAGTGA TATTATGGGA CCAGCTGGGT GAAGGAACTC CTCAGCATCA CCAGCGCAGT 
GTTGAGCTCT TTTATCAGCT ACACAACCTG GTCCCTTCAT CCAGCACCTG TGAGGACGTC 
ATAAGCCAGC AACTCATGCA CAGAGACAAG AGAATTCGTT TGGCAGCCCA CGTGAAGTTC 
TCTGTTCTGT GGCATTTAAC GCGAGACTTG AACATCACCA AGTCTTCTCC GTTTAGTCGC 
ACATTTGACA GGTCTCTCTT CATCATGCTC GACAGCTTAA GTTATTGGGA TCCATGTACC 
AGTGCGGTCG GTCGGGCTTG GTTAAATCAA GTCCTTCAGA GACATGACAT TGCACGGGTT 
CTTGAGCCAC TCCTACTTCT TCTGCTCCAT CCTAAAACTC ACAGGGTTTC TATTCAGAAG 
GTGCAAGCCC AACGCCATTG GGCGCAGATA TTTCCTGATC CACCTGAGCA GGAACCAACA 
GAACCAATCT ACATTAGAGA CTCCGGCTTC TCAGACAACT TTCTTCAGAT TCGAAGCGAA 
GGGGGTGGGC ATGAGGACTT CAGAGGTCTG ACAATAGGTG ACATGGAGCC ATTTTGCCTG 
ACGGTAAACC CCCTAAGTGA CAGCTTGTCC ATTCTTAGCC TGAGCAGTGA AAACTTGCAC 
TTAGCTGGTG AGTATCAGAC CAGGGACCAG CAGGAGGAGC AACAGAGTTC AAAATCAAGT 
GGTTCTCATT CATCTACACT GGAAAACGGT AGCTTTGAAG ACCCTGAGGG GGTCAGCTAC 
ATCGGAAATG GCTCAGATCG ACCGCTTGGT TCATCTGATG AAACGCCTGA CGACTCCATA 
GAGGAAGCTG TGTCTTGTGT AGTGAAGGAC CTGATTGATA GGGTTCTGAA TTTAGTTGAT 
GAGGAATCAA ATGAAACCTC ATCTCCACCT GAAAATTGGC CCCAAACTGA TACTGAAAGC 
ACTTCTTCAG ACACCTCCAC CGGGATCCGC CTGGACTCCT GTCCTCCTCA ATGCTCCAAC 
CACCAGAATC TGCCACAGTT ATTGGCTGAA GGCACACTAG AGTTCCTCTC TGTTACTCCA 
GCGGAGGGGC TACACAAAGA GGGCATCGCC CGCCACAGTT CATCGCCCTC CATCGTCATG 
TTGCCAGACA GCACTGATGC AGCCACTCCA GACCGCAGCC TGCAGGTGGA GGACCCTCAG 
GCCCGCAAAC GTAGCCACAG CAGCACTCAG CTTAGCCTTA AAGGGAAGAT CATGGAGAAG 
CTGGTAGACA AGTCTCCAGG GGCAAAGCCC AAGATCAAGA AGACTAAGAG GAAAGAGGAA 
GAGAAGCAGA AAAAAACGAA CCAAGCTGAG AAACTGCATC CACTCAGTAT TTTCTTTGGA 
GACAGTCTAG ACCTCGAGAA CTGGTACAGC TGTGGTGAAG GGGAGGTGTC GGAGATCGAG 
AGTGACATGG GCTCACCGAG CGGGGGCTCT TGCGGGACCG GTGCTGGGGC GAACGTCACA 
GGGCGCAGAT CGTCCTCCAC TCTGCCTCGT TTTAACATCC ATCCTCTCTA CCAGCACGTC 
CTGCTCTACC TACAGCTCTA CGACTCCTCT AGGACCCTGC ATGCCCTGTC GGCGATCGCA 
GCCATGGTGA GAGCCGCTCC CTCAGGCTTT GTAAGCGCCA TCTCCACCAC CAGCATCAAT 
AACACCTACA CTCCACAGCT CTCCTTGCTG CAAAACCTAC TTGCTCGCCA CAGAGTCTCT 
GTTATGGGCA AAGACTTCTA CTGCCCCATT CCCCAGGACT CTCACTCCCA TTCTTTCCGC 
AGTGCCATGT ACCTGGAGAT CATCATCTCC CTCTGTCTCT ACTTCCTGAG AAGCTATTAC 
TCTGCCCATG TGACATTGGG TCCTCAGGAC TTGGCAGGAA ACCGGGCCAT GCAGCTGACC 
AGCGTGGAGG TCTTGACACT GCTATTCAGC GAACTAGCCA AAGTCACAGC TGGCTCGGCA 
AAGGGTTTTG CTAGCTTCAT CAGTGACGTC CTTTCCAAGT GCAAAGTTCA GAAAGTCATC 
CTCCATTGCC TGCTTTCCTC CATATTCAGC GCCCAGAAAT GGCATGACCA ACGGACGGCA 
GGGGTCAACG TGGCCACGGT GGAGGAAGGT CTCTCGGAGG ACAGCGTCAT CAACATGTCG 
GAGGACCAGA TAGACAGCTG CAGCGCCGTT CAGTCCCAGC TGCTCCGGCT GCTGCAGAGT 
CTAGTTGTAC TTGAGCATAG GGTCTTAATG CCAGCAGACG AAGGTGGAGA AGGAGGACCT 
GCCGCAGGAG GAGCAGGTGG AGGGCCAGGA AGCGGTGGAG TGGCAGGATT TGAGATCCTA 
GGAGGGGAGG TGGAGCATGT TAACCCTCAG CAGCCTATGA CGTCACTGCA GTACCTCCAT 
GGTCAGCCTA TTACAGCTCA GGGCATGTTC CTGTGTGCAG TCATCAGGGC CCTGCACCAG 
CACCATGCCT GTAAGATGCA CCCCCAATGG ATAGGGCTGA TCACAACCAC CCTGCCTTAC 
ATGGGGAGGG TCCTGAGGAG GGTGGTGGCC TCAGTGACTC TGCAGCTATG CAGAAACCTG 
GATAATCTCC TCCAGCAGTA CCGCTATGAA ACCAGCATAA CTGACACCAG ACCCCAGTGG 
ATGGCTCTCT GCATCCCTCC AGACCTGATT CTGACTGTGC TGGAGGGAAT CACTTCCATT 
ATCCATTACT GTCTGCTGGA CCCCACTTCA CAGTACCACC AGTTACAAGT GAGTGTTGAC 
CAGAAGCACC TGGCCGAGGC GCGTTCAGGT ATCCTGTCCA TCCTCCACAC CATCATGTCT 
TCCGTCACGC TGCTGTGGAG CGTGCTCCAC CAAGCCGACA GCTCTGACAA GCCAGCTGCC 
GCTTCAGCTG CCGCCACCTC AAACATCAAC CTAGGCTCCA CCAAGAATCT CCGTCAGCAG 
ATCTTGGAAC TGCTGGGTCC AATCTCCATG AACCACGGCG CTCATTTCAT GGCTGCAATT 
GCTTATGTTT GGAACGAGAG AAAACAAGTC AAGGCGCCAA TCAGAAATAA GGTCATCCCT 
GTAGCGAGTG AGGAGCAGCT GCTGCTGGTT GAGCTCGTTC GGTCGGTCAG CGCCATGCGC 
ACTGAGACTG TTATGCAGAC AGTGAAGGAG GTCCTGAAGC AGCCACCTGC TATCGCAAAA 
GACAAGAAGC ACATCTCTTT GGAAGTCTGC ATGCTACAGT TCTTCTATGC CTATGTACAG 
AGGATTCCTG TTTCCAGTTT GCTTGATAGC TGGCCGTCTC TGCTGGCGCT GCTGAAGGAC 
TCGGTCCAGT TGGGTCTGCC TGCTCCTGGG CAGTTTCTCA TACTGGGAGT TCTGAATGAG 
TTTATCTTGA AGAATCCCAA CCTGGAGAGT AAGAAAGACC AGCGAGAGCT GCAGGATGTG 
ACTCACAAGG TGGTGGAGGC CATCGGCACC ATCGCTGGCT CCTCTTTGGA ACAAACCACG 
TGGCTAAGGA GGAACCTGGA GGTGAAGGCA TCGCCTCAGA TTGTTGTGGA CGGAACCAAC 
CTGGAGGCAG ACGTAGAAGG TGCTCCGTTA CCAAACTTAA TGCTCACAGT GATGGAGGCC 
TCCAGCTTCA CTCCATCTGT GTACAGTGTT CACGCCCTCA CGCTGCTGGC CGAGGTGCTG 
GCTCATCTAT TGGACATGGT GTTCTACAGT GACGAGAAGG AGAAAGTCAT CCCTCTGCTG 
GTTAACATTA TGCACTACGT AGTTCCCTAC CTCCGCAACC ACAGTGCTCA CAATGCCCCC 
AGCTACCGAG CCTGCATCCA GCTGCTGAGC AGCCTGAGCG GCTACCAGTA CACCCGCCGT 
GCCTGGAAAA AAGAGGCCTT CGACCTCTTC ATGGACCACA CCTTCTTCCA GATGGATGCT 
TCGTGTGTCA GCCACTGGAG AGCCATCATT GACCACCTGA TGACTCATGA CAAGACCACA 
TTTAGAGACC TGATGACCCG GGTGGCGGTG GCTCAGAGCA GCTCCCTGAG TTTGTTCACC 
AACAGAGACG CTGAGCTGGA GCAGAGGGCC ATGCTGCTCA AACGCCTGGC CTTCACCATC 
TACAGCAGCG AGGTGGACCA GTACCAGAAG TACCTGCCGG ACATCCAAGA GCGTCTGGTG 
GAGAGCCTGC GTCTGCCTCA GGTACCCATC CTCCACGCTC AGGTGTTCCT GTTCTTCAGA 
GTGCTGCTGC TGCGCATGTC GCCTCAACAC CTCACGTCAC TCTGGCCCAC CATGATCACT 
GAGCTGGTCC AAGTGTTTCT GCTGATGGAG CAGGAGTTAA CCGCTGACGA GGACATATCA 
AGGACGTCAG GACCGTCGGT TGTCGGGTTG GAGACCACCT ACTCTGGAGG GAACGGCTTC 
TCCACGTCCT ACAACAGCCA GCGCTGGCTC AACCTCTACC TCTCTGCTTG CAAGTTCCTG 
GATCTTGCCT TGGCGTTGCC TCCTGAGAGT CTCCCTCAGT TCCAGATGTA CCGCTGGGCC 
TTCATCCCAG AGGCTTCAGA CGATTCAGGG TTGGAAGTGA GACGCCAGGG GACGCATCAG 
AGGGAGTTCA AGCCCTACGT GGTCCGTCTG GTGAAGCTGC TGAGAAAACG AGCCAAGAAA 
AACCCAGAGG AGGACTGTTC CACTAAAACC CTGCCGTGGG AGCCGGGCCA CCTGATGCTG 
ACCCTCTACG TCATCCGCAG CATGGAGCAG CTGCTGCCCT TCTTCAACCT GCTCAGCCAG 
GTCTTCAACA GCAGGGCGAG CTGCCGATCG GGGCCCGTCT ACACCCACAA TCCCGTGGAT 
TCCTCCTTCC CCGCCAGCAA GGAGGGCCAC AAGCTGGAGA GCCAGAAGGT GTTCTGGAGC 
CGAGCCCGAC AGAACATCGA GGAGATGGTG GAAAAAGACT TTCTAGAGGG TCTTATCAAG 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023186392.1
CDS	395..7840
Translation	MNAEEVELLSDSKYRNYVAAVDKALKNFEYSSEWADLISALGKLNKVLQNNAKYQVVPKKLTIGKRLAQCLHPALPSGVHRKALETYEIIFKIIGPKRLAKDLFLYSSGLFPLLSNAAMSVKPVLLGLYETYYLPLGKTLKPGLQGLLTGVLPGLEEGSEYYDRTNTLLEKVAAAVEQSAFYSALWGSILTSPSVRLPGVSFVLLHLNRKLSMEDQLYVMGSDIELMVEAVSTSVQDSSVLVQRSTLDLILFCFPFHMSQATRPDMIRILSAALHVVLRRDMSLNRRLYAWLLGPRSTRQSNPEEHASHYFNSFSKDMLVQAMVGILQGKARGGEEESILMHDLKPFRILISLLDKPELGPAILEDVLIEVFRTLYTQCRTELDLQNQSPFSKDHTHLSSKLRENKKTAELIKTANLLFNSFEPYYMWDYIARWFEECCRRTLNRSTRVARYGWSLDSPDLSLVEFCQLVDFLLDIVSLPTRSMRVICQETYIEIQTEHLPQLLLRMVAALTCHLQALDLGELTHCLRLCSKILSKVQPPLVSPLALPVSPQPPGLAAQNKSRDVDVKQTPPSVPEMTDSGDVFDDGEIPTGSRSAESGFTDFVQYREDGSEDPERASHPHPPLRAGSRPSGSCQSKPLDKPVMQCCLEHFQQFLSCLITFYIVPEHLDKAEGQRGELLHSGPLVSAGSQPSNYTEPCSGAGVVQKDCLAAFTAACQLFLECSSFPVYIAEGNLKSSPAQEEQCDSEQVRLPVWLQTLMDACCLAGDFSLQGVAISLLMDLIGLTQSVAVVTAESLASSSSTEPTQPMSPSQGRVAVVIRPPLTQGILKYIADKTDFFKSVAVILWDQLGEGTPQHHQRSVELFYQLHNLVPSSSTCEDVISQQLMHRDKRIRLAAHVKFSVLWHLTRDLNITKSSPFSRTFDRSLFIMLDSLSYWDPCTSAVGRAWLNQVLQRHDIARVLEPLLLLLLHPKTHRVSIQKVQAQRHWAQIFPDPPEQEPTEPIYIRDSGFSDNFLQIRSEGGGHEDFRGLTIGDMEPFCLTVNPLSDSLSILSLSSENLHLAGEYQTRDQQEEQQSSKSSGSHSSTLENGSFEDPEGVSYIGNGSDRPLGSSDETPDDSIEEAVSCVVKDLIDRVLNLVDEESNETSSPPENWPQTDTESTSSDTSTGIRLDSCPPQCSNHQNLPQLLAEGTLEFLSVTPAEGLHKEGIARHSSSPSIVMLPDSTDAATPDRSLQVEDPQARKRSHSSTQLSLKGKIMEKLVDKSPGAKPKIKKTKRKEEEKQKKTNQAEKLHPLSIFFGDSLDLENWYSCGEGEVSEIESDMGSPSGGSCGTGAGANVTGRRSSSTLPRFNIHPLYQHVLLYLQLYDSSRTLHALSAIAAMVRAAPSGFVSAISTTSINNTYTPQLSLLQNLLARHRVSVMGKDFYCPIPQDSHSHSFRSAMYLEIIISLCLYFLRSYYSAHVTLGPQDLAGNRAMQLTSVEVLTLLFSELAKVTAGSAKGFASFISDVLSKCKVQKVILHCLLSSIFSAQKWHDQRTAGVNVATVEEGLSEDSVINMSEDQIDSCSAVQSQLLRLLQSLVVLEHRVLMPADEGGEGGPAAGGAGGGPGSGGVAGFEILGGEVEHVNPQQPMTSLQYLHGQPITAQGMFLCAVIRALHQHHACKMHPQWIGLITTTLPYMGRVLRRVVASVTLQLCRNLDNLLQQYRYETSITDTRPQWMALCIPPDLILTVLEGITSIIHYCLLDPTSQYHQLQVSVDQKHLAEARSGILSILHTIMSSVTLLWSVLHQADSSDKPAAASAAATSNINLGSTKNLRQQILELLGPISMNHGAHFMAAIAYVWNERKQVKAPIRNKVIPVASEEQLLLVELVRSVSAMRTETVMQTVKEVLKQPPAIAKDKKHISLEVCMLQFFYAYVQRIPVSSLLDSWPSLLALLKDSVQLGLPAPGQFLILGVLNEFILKNPNLESKKDQRELQDVTHKVVEAIGTIAGSSLEQTTWLRRNLEVKASPQIVVDGTNLEADVEGAPLPNLMLTVMEASSFTPSVYSVHALTLLAEVLAHLLDMVFYSDEKEKVIPLLVNIMHYVVPYLRNHSAHNAPSYRACIQLLSSLSGYQYTRRAWKKEAFDLFMDHTFFQMDASCVSHWRAIIDHLMTHDKTTFRDLMTRVAVAQSSSLSLFTNRDAELEQRAMLLKRLAFTIYSSEVDQYQKYLPDIQERLVESLRLPQVPILHAQVFLFFRVLLLRMSPQHLTSLWPTMITELVQVFLLMEQELTADEDISRTSGPSVVGLETTYSGGNGFSTSYNSQRWLNLYLSACKFLDLALALPPESLPQFQMYRWAFIPEASDDSGLEVRRQGTHQREFKPYVVRLVKLLRKRAKKNPEEDCSTKTLPWEPGHLMLTLYVIRSMEQLLPFFNLLSQVFNSRASCRSGPVYTHNPVDSSFPASKEGHKLESQKVFWSRARQNIEEMVEKDFLEGLIKT

Target ORF information:

RefSeq Version	XM_023330624.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023330624.1

ORF Insert Sequence:

ATGAATGCAG AGGAAGTGGA GCTGCTCAGC GACTCAAAGT ACAGGAACTA TGTGGCAGCA 
GTGGACAAAG CCCTCAAGAA CTTTGAGTAT TCCAGCGAGT GGGCCGACCT CATCTCCGCA 
CTGGGCAAGC TCAACAAGGT TTTGCAGAAC AATGCAAAAT ATCAAGTTGT CCCCAAGAAG 
CTGACAATAG GCAAACGGCT GGCCCAGTGC CTCCACCCAG CCCTGCCCAG CGGCGTGCAC 
CGGAAGGCCT TGGAGACCTA CGAGATCATT TTCAAGATCA TTGGGCCCAA GAGGCTGGCC 
AAAGACCTCT TTCTGTACAG CTCGGGACTC TTCCCACTAC TCTCCAACGC TGCTATGTCT 
GTGAAGCCCG TGTTGCTGGG GCTCTATGAG ACCTACTACC TGCCCCTGGG GAAGACTCTC 
AAACCAGGCC TGCAGGGCTT ACTGACCGGC GTGCTGCCTG GTCTGGAGGA AGGTTCAGAG 
TATTACGACA GAACCAACAC GCTGTTGGAG AAAGTTGCTG CAGCCGTGGA GCAGTCGGCC 
TTCTACAGTG CCTTGTGGGG CAGCATCCTG ACCAGCCCCT CTGTGCGTCT CCCCGGGGTC 
TCCTTCGTTC TGCTGCACCT CAACCGGAAG CTCTCCATGG AGGACCAGCT CTACGTCATG 
GGCAGCGACA TTGAGCTTAT GGTGGAGGCT GTTAGTACCT CGGTGCAGGA CTCAAGCGTT 
TTGGTCCAGA GAAGCACTCT GGACCTCATC CTCTTCTGCT TCCCCTTCCA TATGAGCCAG 
GCTACCCGCC CTGACATGAT CCGGATCCTT TCAGCAGCTT TGCATGTGGT TCTGAGAAGA 
GACATGTCTC TGAACCGCAG ACTCTACGCC TGGCTGCTGG GCCCCCGCAG TACTCGGCAG 
AGCAACCCGG AGGAACACGC TTCCCACTAC TTCAACAGCT TCTCCAAGGA CATGCTCGTC 
CAGGCAATGG TTGGGATTCT GCAGGGCAAG GCTCGAGGTG GGGAGGAGGA GAGCATCCTG 
ATGCACGACC TCAAGCCATT TCGCATCCTG ATCAGTCTGC TGGACAAACC AGAACTAGGG 
CCGGCCATTC TAGAGGACGT TCTGATCGAA GTGTTTCGGA CTCTTTACAC ACAGTGCCGG 
ACAGAGTTGG ACCTTCAAAA CCAGAGTCCG TTCAGCAAAG ATCACACACA CCTCAGCAGT 
AAACTGCGAG AAAACAAGAA GACGGCAGAG CTTATCAAAA CAGCCAATCT CCTGTTCAAC 
TCGTTTGAGC CGTACTACAT GTGGGACTAC ATCGCTCGCT GGTTTGAGGA GTGCTGCAGG 
AGAACATTGA ACCGCAGCAC ACGTGTTGCA CGATACGGTT GGAGTCTGGA TTCTCCTGAT 
CTTTCTCTGG TGGAGTTCTG TCAGTTGGTA GATTTTCTAC TAGACATTGT TTCATTGCCT 
ACTAGAAGCA TGAGGGTAAT CTGCCAGGAG ACGTACATAG AAATCCAGAC GGAGCATCTC 
CCTCAACTGC TGTTGCGGAT GGTGGCAGCT CTGACCTGTC ATCTCCAGGC GCTGGACCTC 
GGGGAGCTCA CCCACTGCCT TCGCCTCTGC TCCAAGATCC TCAGCAAGGT GCAGCCTCCG 
TTGGTGTCCC CGCTCGCCCT CCCCGTGAGT CCACAGCCGC CGGGCCTGGC GGCACAGAAC 
AAAAGCAGAG ATGTGGATGT TAAACAGACT CCTCCCTCTG TCCCAGAGAT GACCGACAGT 
GGGGACGTGT TTGACGATGG GGAAATCCCT ACTGGCAGTC GCTCTGCAGA AAGTGGCTTC 
ACAGACTTTG TTCAATACCG AGAAGATGGT TCCGAGGATC CAGAGCGAGC CTCTCATCCC 
CACCCGCCTC TTAGAGCAGG CAGTCGTCCG TCAGGCTCGT GTCAGTCCAA GCCTTTGGAC 
AAACCAGTCA TGCAGTGTTG CCTGGAGCAC TTCCAGCAGT TTCTCTCCTG TCTGATCACT 
TTTTATATTG TTCCTGAGCA CCTGGACAAA GCTGAGGGTC AGAGAGGTGA GCTCTTGCAT 
TCGGGGCCTT TGGTTTCCGC AGGTTCCCAG CCCAGTAATT ATACGGAGCC GTGCTCAGGA 
GCTGGAGTGG TCCAGAAAGA TTGTCTGGCT GCATTTACTG CTGCCTGTCA GCTCTTCTTA 
GAATGCTCCA GTTTCCCAGT CTACATTGCA GAGGGCAACC TCAAGTCCTC GCCTGCACAG 
GAAGAGCAAT GCGACAGTGA GCAGGTTCGG CTGCCAGTGT GGCTGCAGAC CCTGATGGAT 
GCCTGCTGCC TGGCTGGTGA CTTCAGCCTA CAGGGTGTTG CCATCTCCCT GCTTATGGAC 
CTAATAGGCC TGACTCAGTC AGTTGCTGTG GTGACGGCAG AGAGTCTGGC TTCCAGTAGC 
AGTACGGAGC CCACTCAGCC AATGAGCCCC AGCCAGGGTC GGGTGGCTGT GGTCATTAGG 
CCTCCACTCA CTCAGGGAAT CCTGAAGTAT ATTGCGGATA AAACGGACTT CTTCAAGAGC 
GTTGCAGTGA TATTATGGGA CCAGCTGGGT GAAGGAACTC CTCAGCATCA CCAGCGCAGT 
GTTGAGCTCT TTTATCAGCT ACACAACCTG GTCCCTTCAT CCAGCACCTG TGAGGACGTC 
ATAAGCCAGC AACTCATGCA CAGAGACAAG AGAATTCGTT TGGCAGCCCA CGTGAAGTTC 
TCTGTTCTGT GGCATTTAAC GCGAGACTTG AACATCACCA AGTCTTCTCC GTTTAGTCGC 
ACATTTGACA GGTCTCTCTT CATCATGCTC GACAGCTTAA GTTATTGGGA TCCATGTACC 
AGTGCGGTCG GTCGGGCTTG GTTAAATCAA GTCCTTCAGA GACATGACAT TGCACGGGTT 
CTTGAGCCAC TCCTACTTCT TCTGCTCCAT CCTAAAACTC ACAGGGTTTC TATTCAGAAG 
GTGCAAGCCC AACGCCATTG GGCGCAGATA TTTCCTGATC CACCTGAGCA GGAACCAACA 
GAACCAATCT ACATTAGAGA CTCCGGCTTC TCAGACAACT TTCTTCAGAT TCGAAGCGAA 
GGGGGTGGGC ATGAGGACTT CAGAGGTCTG ACAATAGGTG ACATGGAGCC ATTTTGCCTG 
ACGGTAAACC CCCTAAGTGA CAGCTTGTCC ATTCTTAGCC TGAGCAGTGA AAACTTGCAC 
TTAGCTGGTG AGTATCAGAC CAGGGACCAG CAGGAGGAGC AACAGAGTTC AAAATCAAGT 
GGTTCTCATT CATCTACACT GGAAAACGGT AGCTTTGAAG ACCCTGAGGG GGTCAGCTAC 
ATCGGAAATG GCTCAGATCG ACCGCTTGGT TCATCTGATG AAACGCCTGA CGACTCCATA 
GAGGAAGCTG TGTCTTGTGT AGTGAAGGAC CTGATTGATA GGGTTCTGAA TTTAGTTGAT 
GAGGAATCAA ATGAAACCTC ATCTCCACCT GAAAATTGGC CCCAAACTGA TACTGAAAGC 
ACTTCTTCAG ACACCTCCAC CGGGATCCGC CTGGACTCCT GTCCTCCTCA ATGCTCCAAC 
CACCAGAATC TGCCACAGTT ATTGGCTGAA GGCACACTAG AGTTCCTCTC TGTTACTCCA 
GCGGAGGGGC TACACAAAGA GGGCATCGCC CGCCACAGTT CATCGCCCTC CATCGTCATG 
TTGCCAGACA GCACTGATGC AGCCACTCCA GACCGCAGCC TGCAGGTGGA GGACCCTCAG 
GCCCGCAAAC GTAGCCACAG CAGCACTCAG CTTAGCCTTA AAGGGAAGAT CATGGAGAAG 
CTGGTAGACA AGTCTCCAGG GGCAAAGCCC AAGATCAAGA AGACTAAGAG GAAAGAGGAA 
GAGAAGCAGA AAAAAACGAA CCAAGCTGAG AAACTGCATC CACTCAGTAT TTTCTTTGGA 
GACAGTCTAG ACCTCGAGAA CTGGTACAGC TGTGGTGAAG GGGAGGTGTC GGAGATCGAG 
AGTGACATGG GCTCACCGAG CGGGGGCTCT TGCGGGACCG GTGCTGGGGC GAACGTCACA 
GGGCGCAGAT CGTCCTCCAC TCTGCCTCGT TTTAACATCC ATCCTCTCTA CCAGCACGTC 
CTGCTCTACC TACAGCTCTA CGACTCCTCT AGGACCCTGC ATGCCCTGTC GGCGATCGCA 
GCCATGGTGA GAGCCGCTCC CTCAGGCTTT GTAAGCGCCA TCTCCACCAC CAGCATCAAT 
AACACCTACA CTCCACAGCT CTCCTTGCTG CAAAACCTAC TTGCTCGCCA CAGAGTCTCT 
GTTATGGGCA AAGACTTCTA CTGCCCCATT CCCCAGGACT CTCACTCCCA TTCTTTCCGC 
AGTGCCATGT ACCTGGAGAT CATCATCTCC CTCTGTCTCT ACTTCCTGAG AAGCTATTAC 
TCTGCCCATG TGACATTGGG TCCTCAGGAC TTGGCAGGAA ACCGGGCCAT GCAGCTGACC 
AGCGTGGAGG TCTTGACACT GCTATTCAGC GAACTAGCCA AAGTCACAGC TGGCTCGGCA 
AAGGGTTTTG CTAGCTTCAT CAGTGACGTC CTTTCCAAGT GCAAAGTTCA GAAAGTCATC 
CTCCATTGCC TGCTTTCCTC CATATTCAGC GCCCAGAAAT GGCATGACCA ACGGACGGCA 
GGGGTCAACG TGGCCACGGT GGAGGAAGGT CTCTCGGAGG ACAGCGTCAT CAACATGTCG 
GAGGACCAGA TAGACAGCTG CAGCGCCGTT CAGTCCCAGC TGCTCCGGCT GCTGCAGAGT 
CTAGTTGTAC TTGAGCATAG GGTCTTAATG CCAGCAGACG AAGGTGGAGA AGGAGGACCT 
GCCGCAGGAG GAGCAGGTGG AGGGCCAGGA AGCGGTGGAG TGGCAGGATT TGAGATCCTA 
GGAGGGGAGG TGGAGCATGT TAACCCTCAG CAGCCTATGA CGTCACTGCA GTACCTCCAT 
GGTCAGCCTA TTACAGCTCA GGGCATGTTC CTGTGTGCAG TCATCAGGGC CCTGCACCAG 
CACCATGCCT GTAAGATGCA CCCCCAATGG ATAGGGCTGA TCACAACCAC CCTGCCTTAC 
ATGGGGAGGG TCCTGAGGAG GGTGGTGGCC TCAGTGACTC TGCAGCTATG CAGAAACCTG 
GATAATCTCC TCCAGCAGTA CCGCTATGAA ACCAGCATAA CTGACACCAG ACCCCAGTGG 
ATGGCTCTCT GCATCCCTCC AGACCTGATT CTGACTGTGC TGGAGGGAAT CACTTCCATT 
ATCCATTACT GTCTGCTGGA CCCCACTTCA CAGTACCACC AGTTACAAGT GAGTGTTGAC 
CAGAAGCACC TGGCCGAGGC GCGTTCAGGT ATCCTGTCCA TCCTCCACAC CATCATGTCT 
TCCGTCACGC TGCTGTGGAG CGTGCTCCAC CAAGCCGACA GCTCTGACAA GCCAGCTGCC 
GCTTCAGCTG CCGCCACCTC AAACATCAAC CTAGGCTCCA CCAAGAATCT CCGTCAGCAG 
ATCTTGGAAC TGCTGGGTCC AATCTCCATG AACCACGGCG CTCATTTCAT GGCTGCAATT 
GCTTATGTTT GGAACGAGAG AAAACAAGTC AAGGCGCCAA TCAGAAATAA GGTCATCCCT 
GTAGCGAGTG AGGAGCAGCT GCTGCTGGTT GAGCTCGTTC GGTCGGTCAG CGCCATGCGC 
ACTGAGACTG TTATGCAGAC AGTGAAGGAG GTCCTGAAGC AGCCACCTGC TATCGCAAAA 
GACAAGAAGC ACATCTCTTT GGAAGTCTGC ATGCTACAGT TCTTCTATGC CTATGTACAG 
AGGATTCCTG TTTCCAGTTT GCTTGATAGC TGGCCGTCTC TGCTGGCGCT GCTGAAGGAC 
TCGGTCCAGT TGGGTCTGCC TGCTCCTGGG CAGTTTCTCA TACTGGGAGT TCTGAATGAG 
TTTATCTTGA AGAATCCCAA CCTGGAGAGT AAGAAAGACC AGCGAGAGCT GCAGGATGTG 
ACTCACAAGG TGGTGGAGGC CATCGGCACC ATCGCTGGCT CCTCTTTGGA ACAAACCACG 
TGGCTAAGGA GGAACCTGGA GGTGAAGGCA TCGCCTCAGA TTGTTGTGGA CGGAACCAAC 
CTGGAGGCAG ACGTAGAAGG TGCTCCGTTA CCAAACTTAA TGCTCACAGT GATGGAGGCC 
TCCAGCTTCA CTCCATCTGT GTACAGTGTT CACGCCCTCA CGCTGCTGGC CGAGGTGCTG 
GCTCATCTAT TGGACATGGT GTTCTACAGT GACGAGAAGG AGAAAGTCAT CCCTCTGCTG 
GTTAACATTA TGCACTACGT AGTTCCCTAC CTCCGCAACC ACAGTGCTCA CAATGCCCCC 
AGCTACCGAG CCTGCATCCA GCTGCTGAGC AGCCTGAGCG GCTACCAGTA CACCCGCCGT 
GCCTGGAAAA AAGAGGCCTT CGACCTCTTC ATGGACCACA CCTTCTTCCA GATGGATGCT 
TCGTGTGTCA GCCACTGGAG AGCCATCATT GACCACCTGA TGACTCATGA CAAGACCACA 
TTTAGAGACC TGATGACCCG GGTGGCGGTG GCTCAGAGCA GCTCCCTGAG TTTGTTCACC 
AACAGAGACG CTGAGCTGGA GCAGAGGGCC ATGCTGCTCA AACGCCTGGC CTTCACCATC 
TACAGCAGCG AGGTGGACCA GTACCAGAAG TACCTGCCGG ACATCCAAGA GCGTCTGGTG 
GAGAGCCTGC GTCTGCCTCA GGTACCCATC CTCCACGCTC AGGTGTTCCT GTTCTTCAGA 
GTGCTGCTGC TGCGCATGTC GCCTCAACAC CTCACGTCAC TCTGGCCCAC CATGATCACT 
GAGCTGGTCC AAGTGTTTCT GCTGATGGAG CAGGAGTTAA CCGCTGACGA GGACATATCA 
AGGACGTCAG GACCGTCGGT TGTCGGGTTG GAGACCACCT ACTCTGGAGG GAACGGCTTC 
TCCACGTCCT ACAACAGCCA GCGCTGGCTC AACCTCTACC TCTCTGCTTG CAAGTTCCTG 
GATCTTGCCT TGGCGTTGCC TCCTGAGAGT CTCCCTCAGT TCCAGATGTA CCGCTGGGCC 
TTCATCCCAG AGGCTTCAGA CGATTCAGGG TTGGAAGTGA GACGCCAGGG GACGCATCAG 
AGGGAGTTCA AGCCCTACGT GGTCCGTCTG GTGAAGCTGC TGAGAAAACG AGCCAAGAAA 
AACCCAGAGG AGGACTGTTC CACTAAAACC CTGCCGTGGG AGCCGGGCCA CCTGATGCTG 
ACCCTCTACG TCATCCGCAG CATGGAGCAG CTGCTGCCCT TCTTCAACCT GCTCAGCCAG 
GTCTTCAACA GCAGGGCGAG CTGCCGATCG GGGCCCGTCT ACACCCACAA TCCCGTGGAT 
TCCTCCTTCC CCGCCAGCAA GGAGGGCCAC AAGCTGGAGA GCCAGAAGGT GTTCTGGAGC 
CGAGCCCGAC AGAACATCGA GGAGATGGTG GAAAAAGACT TTCTAGAGGG TCTTATCAAG 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOPEY1 ( XM_023330625.1 ) cDNA ORF clone, Xiphophorus maculatus(Southern platyfish) -> XP_023186393.1 Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X5, mRNA.

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CloneID	OXi35842
Clone ID Related Accession (Same CDS sequence)	XM_023330625.1
Accession Version	XM_023330625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	protein dopey-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036445.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAG AGGAAGTGGA GCTGCTCAGC GACTCAAAGT ACAGGAACTA TGTGGCAGCA 
GTGGACAAAG CCCTCAAGAA CTTTGAGTAT TCCAGCGAGT GGGCCGACCT CATCTCCGCA 
CTGGGCAAGC TCAACAAGGT TTTGCAGAAC AATGCAAAAT ATCAAGTTGT CCCCAAGAAG 
CTGACAATAG GCAAACGGCT GGCCCAGTGC CTCCACCCAG CCCTGCCCAG CGGCGTGCAC 
CGGAAGGCCT TGGAGACCTA CGAGATCATT TTCAAGATCA TTGGGCCCAA GAGGCTGGCC 
AAAGACCTCT TTCTGTACAG CTCGGGACTC TTCCCACTAC TCTCCAACGC TGCTATGTCT 
GTGAAGCCCG TGTTGCTGGG GCTCTATGAG ACCTACTACC TGCCCCTGGG GAAGACTCTC 
AAACCAGGCC TGCAGGGCTT ACTGACCGGC GTGCTGCCTG GTCTGGAGGA AGGTTCAGAG 
TATTACGACA GAACCAACAC GCTGTTGGAG AAAGTTGCTG CAGCCGTGGA GCAGTCGGCC 
TTCTACAGTG CCTTGTGGGG CAGCATCCTG ACCAGCCCCT CTGTGCGTCT CCCCGGGGTC 
TCCTTCGTTC TGCTGCACCT CAACCGGAAG CTCTCCATGG AGGACCAGCT CTACGTCATG 
GGCAGCGACA TTGAGCTTAT GGTGGAGGCT GTTAGTACCT CGGTGCAGGA CTCAAGCGTT 
TTGGTCCAGA GAAGCACTCT GGACCTCATC CTCTTCTGCT TCCCCTTCCA TATGAGCCAG 
GCTACCCGCC CTGACATGAT CCGGATCCTT TCAGCAGCTT TGCATGTGGT TCTGAGAAGA 
GACATGTCTC TGAACCGCAG ACTCTACGCC TGGCTGCTGG GCTTTGACAA TAATGGCGGG 
ATGTCAGGCC CCCGCAGTAC TCGGCAGAGC AACCCGGAGG AACACGCTTC CCACTACTTC 
AACAGCTTCT CCAAGGACAT GCTCGTCCAG GCAATGGTTG GGATTCTGCA GGGCAAGGCT 
CGAGGTGGGG AGGAGGAGAG CATCCTGATG CACGACCTCA AGCCATTTCG CATCCTGATC 
AGTCTGCTGG ACAAACCAGA ACTAGGGCCG GCCATTCTAG AGGACGTTCT GATCGAAGTG 
TTTCGGACTC TTTACACACA GTGCCGGACA GAGTTGGACC TTCAAAACCA GAGTCCGTTC 
AGCAAAGATC ACACACACCT CAGCAGTAAA CTGCGAGAAA ACAAGAAGAC GGCAGAGCTT 
ATCAAAACAG CCAATCTCCT GTTCAACTCG TTTGAGCCGT ACTACATGTG GGACTACATC 
GCTCGCTGGT TTGAGGAGTG CTGCAGGAGA ACATTGAACC GCAGCACACG TGTTGCACGA 
TACGGTTGGA GTCTGGATTC TCCTGATCTT TCTCTGGTGG AGTTCTGTCA GTTGGTAGAT 
TTTCTACTAG ACATTGTTTC ATTGGAGACG TACATAGAAA TCCAGACGGA GCATCTCCCT 
CAACTGCTGT TGCGGATGGT GGCAGCTCTG ACCTGTCATC TCCAGGCGCT GGACCTCGGG 
GAGCTCACCC ACTGCCTTCG CCTCTGCTCC AAGATCCTCA GCAAGGTGCA GCCTCCGTTG 
GTGTCCCCGC TCGCCCTCCC CGTGAGTCCA CAGCCGCCGG GCCTGGCGGC ACAGAACAAA 
AGCAGAGATG TGGATGTTAA ACAGACTCCT CCCTCTGTCC CAGAGATGAC CGACAGTGGG 
GACGTGTTTG ACGATGGGGA AATCCCTACT GGCAGTCGCT CTGCAGAAAG TGGCTTCACA 
GACTTTGTTC AATACCGAGA AGATGGTTCC GAGGATCCAG AGCGAGCCTC TCATCCCCAC 
CCGCCTCTTA GAGCAGGCAG TCGTCCGTCA GGCTCGTGTC AGTCCAAGCC TTTGGACAAA 
CCAGTCATGC AGTGTTGCCT GGAGCACTTC CAGCAGTTTC TCTCCTGTCT GATCACTTTT 
TATATTGTTC CTGAGCACCT GGACAAAGCT GAGGGTCAGA GAGGTGAGCT CTTGCATTCG 
GGGCCTTTGG TTTCCGCAGG TTCCCAGCCC AGTAATTATA CGGAGCCGTG CTCAGGAGCT 
GGAGTGGTCC AGAAAGATTG TCTGGCTGCA TTTACTGCTG CCTGTCAGCT CTTCTTAGAA 
TGCTCCAGTT TCCCAGTCTA CATTGCAGAG GGCAACCTCA AGTCCTCGCC TGCACAGGAA 
GAGCAATGCG ACAGTGAGCA GGTTCGGCTG CCAGTGTGGC TGCAGACCCT GATGGATGCC 
TGCTGCCTGG CTGGTGACTT CAGCCTACAG GGTGTTGCCA TCTCCCTGCT TATGGACCTA 
ATAGGCCTGA CTCAGTCAGT TGCTGTGGTG ACGGCAGAGA GTCTGGCTTC CAGTAGCAGT 
ACGGAGCCCA CTCAGCCAAT GAGCCCCAGC CAGGGTCGGG TGGCTGTGGT CATTAGGCCT 
CCACTCACTC AGGGAATCCT GAAGTATATT GCGGATAAAA CGGACTTCTT CAAGAGCGTT 
GCAGTGATAT TATGGGACCA GCTGGGTGAA GGAACTCCTC AGCATCACCA GCGCAGTGTT 
GAGCTCTTTT ATCAGCTACA CAACCTGGTC CCTTCATCCA GCACCTGTGA GGACGTCATA 
AGCCAGCAAC TCATGCACAG AGACAAGAGA ATTCGTTTGG CAGCCCACGT GAAGTTCTCT 
GTTCTGTGGC ATTTAACGCG AGACTTGAAC ATCACCAAGT CTTCTCCGTT TAGTCGCACA 
TTTGACAGGT CTCTCTTCAT CATGCTCGAC AGCTTAAGTT ATTGGGATCC ATGTACCAGT 
GCGGTCGGTC GGGCTTGGTT AAATCAAGTC CTTCAGAGAC ATGACATTGC ACGGGTTCTT 
GAGCCACTCC TACTTCTTCT GCTCCATCCT AAAACTCACA GGGTTTCTAT TCAGAAGGTG 
CAAGCCCAAC GCCATTGGGC GCAGATATTT CCTGATCCAC CTGAGCAGGA ACCAACAGAA 
CCAATCTACA TTAGAGACTC CGGCTTCTCA GACAACTTTC TTCAGATTCG AAGCGAAGGG 
GGTGGGCATG AGGACTTCAG AGGTCTGACA ATAGGTGACA TGGAGCCATT TTGCCTGACG 
GTAAACCCCC TAAGTGACAG CTTGTCCATT CTTAGCCTGA GCAGTGAAAA CTTGCACTTA 
GCTGGTGAGT ATCAGACCAG GGACCAGCAG GAGGAGCAAC AGAGTTCAAA ATCAAGTGGT 
TCTCATTCAT CTACACTGGA AAACGGTAGC TTTGAAGACC CTGAGGGGGT CAGCTACATC 
GGAAATGGCT CAGATCGACC GCTTGGTTCA TCTGATGAAA CGCCTGACGA CTCCATAGAG 
GAAGCTGTGT CTTGTGTAGT GAAGGACCTG ATTGATAGGG TTCTGAATTT AGTTGATGAG 
GAATCAAATG AAACCTCATC TCCACCTGAA AATTGGCCCC AAACTGATAC TGAAAGCACT 
TCTTCAGACA CCTCCACCGG GATCCGCCTG GACTCCTGTC CTCCTCAATG CTCCAACCAC 
CAGAATCTGC CACAGTTATT GGCTGAAGGC ACACTAGAGT TCCTCTCTGT TACTCCAGCG 
GAGGGGCTAC ACAAAGAGGG CATCGCCCGC CACAGTTCAT CGCCCTCCAT CGTCATGTTG 
CCAGACAGCA CTGATGCAGC CACTCCAGAC CGCAGCCTGC AGGTGGAGGA CCCTCAGGCC 
CGCAAACGTA GCCACAGCAG CACTCAGCTT AGCCTTAAAG GGAAGATCAT GGAGAAGCTG 
GTAGACAAGT CTCCAGGGGC AAAGCCCAAG ATCAAGAAGA CTAAGAGGAA AGAGGAAGAG 
AAGCAGAAAA AAACGAACCA AGCTGAGAAA CTGCATCCAC TCAGTATTTT CTTTGGAGAC 
AGTCTAGACC TCGAGAACTG GTACAGCTGT GGTGAAGGGG AGGTGTCGGA GATCGAGAGT 
GACATGGGCT CACCGAGCGG GGGCTCTTGC GGGACCGGTG CTGGGGCGAA CGTCACAGGG 
CGCAGATCGT CCTCCACTCT GCCTCGTTTT AACATCCATC CTCTCTACCA GCACGTCCTG 
CTCTACCTAC AGCTCTACGA CTCCTCTAGG ACCCTGCATG CCCTGTCGGC GATCGCAGCC 
ATGGTGAGAG CCGCTCCCTC AGGCTTTGTA AGCGCCATCT CCACCACCAG CATCAATAAC 
ACCTACACTC CACAGCTCTC CTTGCTGCAA AACCTACTTG CTCGCCACAG AGTCTCTGTT 
ATGGGCAAAG ACTTCTACTG CCCCATTCCC CAGGACTCTC ACTCCCATTC TTTCCGCAGT 
GCCATGTACC TGGAGATCAT CATCTCCCTC TGTCTCTACT TCCTGAGAAG CTATTACTCT 
GCCCATGTGA CATTGGGTCC TCAGGACTTG GCAGGAAACC GGGCCATGCA GCTGACCAGC 
GTGGAGGTCT TGACACTGCT ATTCAGCGAA CTAGCCAAAG TCACAGCTGG CTCGGCAAAG 
GGTTTTGCTA GCTTCATCAG TGACGTCCTT TCCAAGTGCA AAGTTCAGAA AGTCATCCTC 
CATTGCCTGC TTTCCTCCAT ATTCAGCGCC CAGAAATGGC ATGACCAACG GACGGCAGGG 
GTCAACGTGG CCACGGTGGA GGAAGGTCTC TCGGAGGACA GCGTCATCAA CATGTCGGAG 
GACCAGATAG ACAGCTGCAG CGCCGTTCAG TCCCAGCTGC TCCGGCTGCT GCAGAGTCTA 
GTTGTACTTG AGCATAGGGT CTTAATGCCA GCAGACGAAG GTGGAGAAGG AGGACCTGCC 
GCAGGAGGAG CAGGTGGAGG GCCAGGAAGC GGTGGAGTGG CAGGATTTGA GATCCTAGGA 
GGGGAGGTGG AGCATGTTAA CCCTCAGCAG CCTATGACGT CACTGCAGTA CCTCCATGGT 
CAGCCTATTA CAGCTCAGGG CATGTTCCTG TGTGCAGTCA TCAGGGCCCT GCACCAGCAC 
CATGCCTGTA AGATGCACCC CCAATGGATA GGGCTGATCA CAACCACCCT GCCTTACATG 
GGGAGGGTCC TGAGGAGGGT GGTGGCCTCA GTGACTCTGC AGCTATGCAG AAACCTGGAT 
AATCTCCTCC AGCAGTACCG CTATGAAACC AGCATAACTG ACACCAGACC CCAGTGGATG 
GCTCTCTGCA TCCCTCCAGA CCTGATTCTG ACTGTGCTGG AGGGAATCAC TTCCATTATC 
CATTACTGTC TGCTGGACCC CACTTCACAG TACCACCAGT TACAAGTGAG TGTTGACCAG 
AAGCACCTGG CCGAGGCGCG TTCAGGTATC CTGTCCATCC TCCACACCAT CATGTCTTCC 
GTCACGCTGC TGTGGAGCGT GCTCCACCAA GCCGACAGCT CTGACAAGCC AGCTGCCGCT 
TCAGCTGCCG CCACCTCAAA CATCAACCTA GGCTCCACCA AGAATCTCCG TCAGCAGATC 
TTGGAACTGC TGGGTCCAAT CTCCATGAAC CACGGCGCTC ATTTCATGGC TGCAATTGCT 
TATGTTTGGA ACGAGAGAAA ACAAGTCAAG GCGCCAATCA GAAATAAGGT CATCCCTGTA 
GCGAGTGAGG AGCAGCTGCT GCTGGTTGAG CTCGTTCGGT CGGTCAGCGC CATGCGCACT 
GAGACTGTTA TGCAGACAGT GAAGGAGGTC CTGAAGCAGC CACCTGCTAT CGCAAAAGAC 
AAGAAGCACA TCTCTTTGGA AGTCTGCATG CTACAGTTCT TCTATGCCTA TGTACAGAGG 
ATTCCTGTTT CCAGTTTGCT TGATAGCTGG CCGTCTCTGC TGGCGCTGCT GAAGGACTCG 
GTCCAGTTGG GTCTGCCTGC TCCTGGGCAG TTTCTCATAC TGGGAGTTCT GAATGAGTTT 
ATCTTGAAGA ATCCCAACCT GGAGAGTAAG AAAGACCAGC GAGAGCTGCA GGATGTGACT 
CACAAGGTGG TGGAGGCCAT CGGCACCATC GCTGGCTCCT CTTTGGAACA AACCACGTGG 
CTAAGGAGGA ACCTGGAGGT GAAGGCATCG CCTCAGATTG TTGTGGACGG AACCAACCTG 
GAGGCAGACG TAGAAGGTGC TCCGTTACCA AACTTAATGC TCACAGTGAT GGAGGCCTCC 
AGCTTCACTC CATCTGTGTA CAGTGTTCAC GCCCTCACGC TGCTGGCCGA GGTGCTGGCT 
CATCTATTGG ACATGGTGTT CTACAGTGAC GAGAAGGAGA AAGTCATCCC TCTGCTGGTT 
AACATTATGC ACTACGTAGT TCCCTACCTC CGCAACCACA GTGCTCACAA TGCCCCCAGC 
TACCGAGCCT GCATCCAGCT GCTGAGCAGC CTGAGCGGCT ACCAGTACAC CCGCCGTGCC 
TGGAAAAAAG AGGCCTTCGA CCTCTTCATG GACCACACCT TCTTCCAGAT GGATGCTTCG 
TGTGTCAGCC ACTGGAGAGC CATCATTGAC CACCTGATGA CTCATGACAA GACCACATTT 
AGAGACCTGA TGACCCGGGT GGCGGTGGCT CAGAGCAGCT CCCTGAGTTT GTTCACCAAC 
AGAGACGCTG AGCTGGAGCA GAGGGCCATG CTGCTCAAAC GCCTGGCCTT CACCATCTAC 
AGCAGCGAGG TGGACCAGTA CCAGAAGTAC CTGCCGGACA TCCAAGAGCG TCTGGTGGAG 
AGCCTGCGTC TGCCTCAGGT ACCCATCCTC CACGCTCAGG TGTTCCTGTT CTTCAGAGTG 
CTGCTGCTGC GCATGTCGCC TCAACACCTC ACGTCACTCT GGCCCACCAT GATCACTGAG 
CTGGTCCAAG TGTTTCTGCT GATGGAGCAG GAGTTAACCG CTGACGAGGA CATATCAAGG 
ACGTCAGGAC CGTCGGTTGT CGGGTTGGAG ACCACCTACT CTGGAGGGAA CGGCTTCTCC 
ACGTCCTACA ACAGCCAGCG CTGGCTCAAC CTCTACCTCT CTGCTTGCAA GTTCCTGGAT 
CTTGCCTTGG CGTTGCCTCC TGAGAGTCTC CCTCAGTTCC AGATGTACCG CTGGGCCTTC 
ATCCCAGAGG CTTCAGACGA TTCAGGGTTG GAAGTGAGAC GCCAGGGGAC GCATCAGAGG 
GAGTTCAAGC CCTACGTGGT CCGTCTGGTG AAGCTGCTGA GAAAACGAGC CAAGAAAAAC 
CCAGAGGAGG ACTGTTCCAC TAAAACCCTG CCGTGGGAGC CGGGCCACCT GATGCTGACC 
CTCTACGTCA TCCGCAGCAT GGAGCAGCTG CTGCCCTTCT TCAACCTGCT CAGCCAGGTC 
TTCAACAGCA GGGCGAGCTG CCGATCGGGG CCCGTCTACA CCCACAATCC CGTGGATTCC 
TCCTTCCCCG CCAGCAAGGA GGGCCACAAG CTGGAGAGCC AGAAGGTGTT CTGGAGCCGA 
GCCCGACAGA ACATCGAGGA GATGGTGGAA AAAGACTTTC TAGAGGGTCT TATCAAGACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023186393.1
CDS	395..7837
Translation	MNAEEVELLSDSKYRNYVAAVDKALKNFEYSSEWADLISALGKLNKVLQNNAKYQVVPKKLTIGKRLAQCLHPALPSGVHRKALETYEIIFKIIGPKRLAKDLFLYSSGLFPLLSNAAMSVKPVLLGLYETYYLPLGKTLKPGLQGLLTGVLPGLEEGSEYYDRTNTLLEKVAAAVEQSAFYSALWGSILTSPSVRLPGVSFVLLHLNRKLSMEDQLYVMGSDIELMVEAVSTSVQDSSVLVQRSTLDLILFCFPFHMSQATRPDMIRILSAALHVVLRRDMSLNRRLYAWLLGFDNNGGMSGPRSTRQSNPEEHASHYFNSFSKDMLVQAMVGILQGKARGGEEESILMHDLKPFRILISLLDKPELGPAILEDVLIEVFRTLYTQCRTELDLQNQSPFSKDHTHLSSKLRENKKTAELIKTANLLFNSFEPYYMWDYIARWFEECCRRTLNRSTRVARYGWSLDSPDLSLVEFCQLVDFLLDIVSLETYIEIQTEHLPQLLLRMVAALTCHLQALDLGELTHCLRLCSKILSKVQPPLVSPLALPVSPQPPGLAAQNKSRDVDVKQTPPSVPEMTDSGDVFDDGEIPTGSRSAESGFTDFVQYREDGSEDPERASHPHPPLRAGSRPSGSCQSKPLDKPVMQCCLEHFQQFLSCLITFYIVPEHLDKAEGQRGELLHSGPLVSAGSQPSNYTEPCSGAGVVQKDCLAAFTAACQLFLECSSFPVYIAEGNLKSSPAQEEQCDSEQVRLPVWLQTLMDACCLAGDFSLQGVAISLLMDLIGLTQSVAVVTAESLASSSSTEPTQPMSPSQGRVAVVIRPPLTQGILKYIADKTDFFKSVAVILWDQLGEGTPQHHQRSVELFYQLHNLVPSSSTCEDVISQQLMHRDKRIRLAAHVKFSVLWHLTRDLNITKSSPFSRTFDRSLFIMLDSLSYWDPCTSAVGRAWLNQVLQRHDIARVLEPLLLLLLHPKTHRVSIQKVQAQRHWAQIFPDPPEQEPTEPIYIRDSGFSDNFLQIRSEGGGHEDFRGLTIGDMEPFCLTVNPLSDSLSILSLSSENLHLAGEYQTRDQQEEQQSSKSSGSHSSTLENGSFEDPEGVSYIGNGSDRPLGSSDETPDDSIEEAVSCVVKDLIDRVLNLVDEESNETSSPPENWPQTDTESTSSDTSTGIRLDSCPPQCSNHQNLPQLLAEGTLEFLSVTPAEGLHKEGIARHSSSPSIVMLPDSTDAATPDRSLQVEDPQARKRSHSSTQLSLKGKIMEKLVDKSPGAKPKIKKTKRKEEEKQKKTNQAEKLHPLSIFFGDSLDLENWYSCGEGEVSEIESDMGSPSGGSCGTGAGANVTGRRSSSTLPRFNIHPLYQHVLLYLQLYDSSRTLHALSAIAAMVRAAPSGFVSAISTTSINNTYTPQLSLLQNLLARHRVSVMGKDFYCPIPQDSHSHSFRSAMYLEIIISLCLYFLRSYYSAHVTLGPQDLAGNRAMQLTSVEVLTLLFSELAKVTAGSAKGFASFISDVLSKCKVQKVILHCLLSSIFSAQKWHDQRTAGVNVATVEEGLSEDSVINMSEDQIDSCSAVQSQLLRLLQSLVVLEHRVLMPADEGGEGGPAAGGAGGGPGSGGVAGFEILGGEVEHVNPQQPMTSLQYLHGQPITAQGMFLCAVIRALHQHHACKMHPQWIGLITTTLPYMGRVLRRVVASVTLQLCRNLDNLLQQYRYETSITDTRPQWMALCIPPDLILTVLEGITSIIHYCLLDPTSQYHQLQVSVDQKHLAEARSGILSILHTIMSSVTLLWSVLHQADSSDKPAAASAAATSNINLGSTKNLRQQILELLGPISMNHGAHFMAAIAYVWNERKQVKAPIRNKVIPVASEEQLLLVELVRSVSAMRTETVMQTVKEVLKQPPAIAKDKKHISLEVCMLQFFYAYVQRIPVSSLLDSWPSLLALLKDSVQLGLPAPGQFLILGVLNEFILKNPNLESKKDQRELQDVTHKVVEAIGTIAGSSLEQTTWLRRNLEVKASPQIVVDGTNLEADVEGAPLPNLMLTVMEASSFTPSVYSVHALTLLAEVLAHLLDMVFYSDEKEKVIPLLVNIMHYVVPYLRNHSAHNAPSYRACIQLLSSLSGYQYTRRAWKKEAFDLFMDHTFFQMDASCVSHWRAIIDHLMTHDKTTFRDLMTRVAVAQSSSLSLFTNRDAELEQRAMLLKRLAFTIYSSEVDQYQKYLPDIQERLVESLRLPQVPILHAQVFLFFRVLLLRMSPQHLTSLWPTMITELVQVFLLMEQELTADEDISRTSGPSVVGLETTYSGGNGFSTSYNSQRWLNLYLSACKFLDLALALPPESLPQFQMYRWAFIPEASDDSGLEVRRQGTHQREFKPYVVRLVKLLRKRAKKNPEEDCSTKTLPWEPGHLMLTLYVIRSMEQLLPFFNLLSQVFNSRASCRSGPVYTHNPVDSSFPASKEGHKLESQKVFWSRARQNIEEMVEKDFLEGLIKT

Target ORF information:

RefSeq Version	XM_023330625.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023330625.1

ORF Insert Sequence:

ATGAATGCAG AGGAAGTGGA GCTGCTCAGC GACTCAAAGT ACAGGAACTA TGTGGCAGCA 
GTGGACAAAG CCCTCAAGAA CTTTGAGTAT TCCAGCGAGT GGGCCGACCT CATCTCCGCA 
CTGGGCAAGC TCAACAAGGT TTTGCAGAAC AATGCAAAAT ATCAAGTTGT CCCCAAGAAG 
CTGACAATAG GCAAACGGCT GGCCCAGTGC CTCCACCCAG CCCTGCCCAG CGGCGTGCAC 
CGGAAGGCCT TGGAGACCTA CGAGATCATT TTCAAGATCA TTGGGCCCAA GAGGCTGGCC 
AAAGACCTCT TTCTGTACAG CTCGGGACTC TTCCCACTAC TCTCCAACGC TGCTATGTCT 
GTGAAGCCCG TGTTGCTGGG GCTCTATGAG ACCTACTACC TGCCCCTGGG GAAGACTCTC 
AAACCAGGCC TGCAGGGCTT ACTGACCGGC GTGCTGCCTG GTCTGGAGGA AGGTTCAGAG 
TATTACGACA GAACCAACAC GCTGTTGGAG AAAGTTGCTG CAGCCGTGGA GCAGTCGGCC 
TTCTACAGTG CCTTGTGGGG CAGCATCCTG ACCAGCCCCT CTGTGCGTCT CCCCGGGGTC 
TCCTTCGTTC TGCTGCACCT CAACCGGAAG CTCTCCATGG AGGACCAGCT CTACGTCATG 
GGCAGCGACA TTGAGCTTAT GGTGGAGGCT GTTAGTACCT CGGTGCAGGA CTCAAGCGTT 
TTGGTCCAGA GAAGCACTCT GGACCTCATC CTCTTCTGCT TCCCCTTCCA TATGAGCCAG 
GCTACCCGCC CTGACATGAT CCGGATCCTT TCAGCAGCTT TGCATGTGGT TCTGAGAAGA 
GACATGTCTC TGAACCGCAG ACTCTACGCC TGGCTGCTGG GCTTTGACAA TAATGGCGGG 
ATGTCAGGCC CCCGCAGTAC TCGGCAGAGC AACCCGGAGG AACACGCTTC CCACTACTTC 
AACAGCTTCT CCAAGGACAT GCTCGTCCAG GCAATGGTTG GGATTCTGCA GGGCAAGGCT 
CGAGGTGGGG AGGAGGAGAG CATCCTGATG CACGACCTCA AGCCATTTCG CATCCTGATC 
AGTCTGCTGG ACAAACCAGA ACTAGGGCCG GCCATTCTAG AGGACGTTCT GATCGAAGTG 
TTTCGGACTC TTTACACACA GTGCCGGACA GAGTTGGACC TTCAAAACCA GAGTCCGTTC 
AGCAAAGATC ACACACACCT CAGCAGTAAA CTGCGAGAAA ACAAGAAGAC GGCAGAGCTT 
ATCAAAACAG CCAATCTCCT GTTCAACTCG TTTGAGCCGT ACTACATGTG GGACTACATC 
GCTCGCTGGT TTGAGGAGTG CTGCAGGAGA ACATTGAACC GCAGCACACG TGTTGCACGA 
TACGGTTGGA GTCTGGATTC TCCTGATCTT TCTCTGGTGG AGTTCTGTCA GTTGGTAGAT 
TTTCTACTAG ACATTGTTTC ATTGGAGACG TACATAGAAA TCCAGACGGA GCATCTCCCT 
CAACTGCTGT TGCGGATGGT GGCAGCTCTG ACCTGTCATC TCCAGGCGCT GGACCTCGGG 
GAGCTCACCC ACTGCCTTCG CCTCTGCTCC AAGATCCTCA GCAAGGTGCA GCCTCCGTTG 
GTGTCCCCGC TCGCCCTCCC CGTGAGTCCA CAGCCGCCGG GCCTGGCGGC ACAGAACAAA 
AGCAGAGATG TGGATGTTAA ACAGACTCCT CCCTCTGTCC CAGAGATGAC CGACAGTGGG 
GACGTGTTTG ACGATGGGGA AATCCCTACT GGCAGTCGCT CTGCAGAAAG TGGCTTCACA 
GACTTTGTTC AATACCGAGA AGATGGTTCC GAGGATCCAG AGCGAGCCTC TCATCCCCAC 
CCGCCTCTTA GAGCAGGCAG TCGTCCGTCA GGCTCGTGTC AGTCCAAGCC TTTGGACAAA 
CCAGTCATGC AGTGTTGCCT GGAGCACTTC CAGCAGTTTC TCTCCTGTCT GATCACTTTT 
TATATTGTTC CTGAGCACCT GGACAAAGCT GAGGGTCAGA GAGGTGAGCT CTTGCATTCG 
GGGCCTTTGG TTTCCGCAGG TTCCCAGCCC AGTAATTATA CGGAGCCGTG CTCAGGAGCT 
GGAGTGGTCC AGAAAGATTG TCTGGCTGCA TTTACTGCTG CCTGTCAGCT CTTCTTAGAA 
TGCTCCAGTT TCCCAGTCTA CATTGCAGAG GGCAACCTCA AGTCCTCGCC TGCACAGGAA 
GAGCAATGCG ACAGTGAGCA GGTTCGGCTG CCAGTGTGGC TGCAGACCCT GATGGATGCC 
TGCTGCCTGG CTGGTGACTT CAGCCTACAG GGTGTTGCCA TCTCCCTGCT TATGGACCTA 
ATAGGCCTGA CTCAGTCAGT TGCTGTGGTG ACGGCAGAGA GTCTGGCTTC CAGTAGCAGT 
ACGGAGCCCA CTCAGCCAAT GAGCCCCAGC CAGGGTCGGG TGGCTGTGGT CATTAGGCCT 
CCACTCACTC AGGGAATCCT GAAGTATATT GCGGATAAAA CGGACTTCTT CAAGAGCGTT 
GCAGTGATAT TATGGGACCA GCTGGGTGAA GGAACTCCTC AGCATCACCA GCGCAGTGTT 
GAGCTCTTTT ATCAGCTACA CAACCTGGTC CCTTCATCCA GCACCTGTGA GGACGTCATA 
AGCCAGCAAC TCATGCACAG AGACAAGAGA ATTCGTTTGG CAGCCCACGT GAAGTTCTCT 
GTTCTGTGGC ATTTAACGCG AGACTTGAAC ATCACCAAGT CTTCTCCGTT TAGTCGCACA 
TTTGACAGGT CTCTCTTCAT CATGCTCGAC AGCTTAAGTT ATTGGGATCC ATGTACCAGT 
GCGGTCGGTC GGGCTTGGTT AAATCAAGTC CTTCAGAGAC ATGACATTGC ACGGGTTCTT 
GAGCCACTCC TACTTCTTCT GCTCCATCCT AAAACTCACA GGGTTTCTAT TCAGAAGGTG 
CAAGCCCAAC GCCATTGGGC GCAGATATTT CCTGATCCAC CTGAGCAGGA ACCAACAGAA 
CCAATCTACA TTAGAGACTC CGGCTTCTCA GACAACTTTC TTCAGATTCG AAGCGAAGGG 
GGTGGGCATG AGGACTTCAG AGGTCTGACA ATAGGTGACA TGGAGCCATT TTGCCTGACG 
GTAAACCCCC TAAGTGACAG CTTGTCCATT CTTAGCCTGA GCAGTGAAAA CTTGCACTTA 
GCTGGTGAGT ATCAGACCAG GGACCAGCAG GAGGAGCAAC AGAGTTCAAA ATCAAGTGGT 
TCTCATTCAT CTACACTGGA AAACGGTAGC TTTGAAGACC CTGAGGGGGT CAGCTACATC 
GGAAATGGCT CAGATCGACC GCTTGGTTCA TCTGATGAAA CGCCTGACGA CTCCATAGAG 
GAAGCTGTGT CTTGTGTAGT GAAGGACCTG ATTGATAGGG TTCTGAATTT AGTTGATGAG 
GAATCAAATG AAACCTCATC TCCACCTGAA AATTGGCCCC AAACTGATAC TGAAAGCACT 
TCTTCAGACA CCTCCACCGG GATCCGCCTG GACTCCTGTC CTCCTCAATG CTCCAACCAC 
CAGAATCTGC CACAGTTATT GGCTGAAGGC ACACTAGAGT TCCTCTCTGT TACTCCAGCG 
GAGGGGCTAC ACAAAGAGGG CATCGCCCGC CACAGTTCAT CGCCCTCCAT CGTCATGTTG 
CCAGACAGCA CTGATGCAGC CACTCCAGAC CGCAGCCTGC AGGTGGAGGA CCCTCAGGCC 
CGCAAACGTA GCCACAGCAG CACTCAGCTT AGCCTTAAAG GGAAGATCAT GGAGAAGCTG 
GTAGACAAGT CTCCAGGGGC AAAGCCCAAG ATCAAGAAGA CTAAGAGGAA AGAGGAAGAG 
AAGCAGAAAA AAACGAACCA AGCTGAGAAA CTGCATCCAC TCAGTATTTT CTTTGGAGAC 
AGTCTAGACC TCGAGAACTG GTACAGCTGT GGTGAAGGGG AGGTGTCGGA GATCGAGAGT 
GACATGGGCT CACCGAGCGG GGGCTCTTGC GGGACCGGTG CTGGGGCGAA CGTCACAGGG 
CGCAGATCGT CCTCCACTCT GCCTCGTTTT AACATCCATC CTCTCTACCA GCACGTCCTG 
CTCTACCTAC AGCTCTACGA CTCCTCTAGG ACCCTGCATG CCCTGTCGGC GATCGCAGCC 
ATGGTGAGAG CCGCTCCCTC AGGCTTTGTA AGCGCCATCT CCACCACCAG CATCAATAAC 
ACCTACACTC CACAGCTCTC CTTGCTGCAA AACCTACTTG CTCGCCACAG AGTCTCTGTT 
ATGGGCAAAG ACTTCTACTG CCCCATTCCC CAGGACTCTC ACTCCCATTC TTTCCGCAGT 
GCCATGTACC TGGAGATCAT CATCTCCCTC TGTCTCTACT TCCTGAGAAG CTATTACTCT 
GCCCATGTGA CATTGGGTCC TCAGGACTTG GCAGGAAACC GGGCCATGCA GCTGACCAGC 
GTGGAGGTCT TGACACTGCT ATTCAGCGAA CTAGCCAAAG TCACAGCTGG CTCGGCAAAG 
GGTTTTGCTA GCTTCATCAG TGACGTCCTT TCCAAGTGCA AAGTTCAGAA AGTCATCCTC 
CATTGCCTGC TTTCCTCCAT ATTCAGCGCC CAGAAATGGC ATGACCAACG GACGGCAGGG 
GTCAACGTGG CCACGGTGGA GGAAGGTCTC TCGGAGGACA GCGTCATCAA CATGTCGGAG 
GACCAGATAG ACAGCTGCAG CGCCGTTCAG TCCCAGCTGC TCCGGCTGCT GCAGAGTCTA 
GTTGTACTTG AGCATAGGGT CTTAATGCCA GCAGACGAAG GTGGAGAAGG AGGACCTGCC 
GCAGGAGGAG CAGGTGGAGG GCCAGGAAGC GGTGGAGTGG CAGGATTTGA GATCCTAGGA 
GGGGAGGTGG AGCATGTTAA CCCTCAGCAG CCTATGACGT CACTGCAGTA CCTCCATGGT 
CAGCCTATTA CAGCTCAGGG CATGTTCCTG TGTGCAGTCA TCAGGGCCCT GCACCAGCAC 
CATGCCTGTA AGATGCACCC CCAATGGATA GGGCTGATCA CAACCACCCT GCCTTACATG 
GGGAGGGTCC TGAGGAGGGT GGTGGCCTCA GTGACTCTGC AGCTATGCAG AAACCTGGAT 
AATCTCCTCC AGCAGTACCG CTATGAAACC AGCATAACTG ACACCAGACC CCAGTGGATG 
GCTCTCTGCA TCCCTCCAGA CCTGATTCTG ACTGTGCTGG AGGGAATCAC TTCCATTATC 
CATTACTGTC TGCTGGACCC CACTTCACAG TACCACCAGT TACAAGTGAG TGTTGACCAG 
AAGCACCTGG CCGAGGCGCG TTCAGGTATC CTGTCCATCC TCCACACCAT CATGTCTTCC 
GTCACGCTGC TGTGGAGCGT GCTCCACCAA GCCGACAGCT CTGACAAGCC AGCTGCCGCT 
TCAGCTGCCG CCACCTCAAA CATCAACCTA GGCTCCACCA AGAATCTCCG TCAGCAGATC 
TTGGAACTGC TGGGTCCAAT CTCCATGAAC CACGGCGCTC ATTTCATGGC TGCAATTGCT 
TATGTTTGGA ACGAGAGAAA ACAAGTCAAG GCGCCAATCA GAAATAAGGT CATCCCTGTA 
GCGAGTGAGG AGCAGCTGCT GCTGGTTGAG CTCGTTCGGT CGGTCAGCGC CATGCGCACT 
GAGACTGTTA TGCAGACAGT GAAGGAGGTC CTGAAGCAGC CACCTGCTAT CGCAAAAGAC 
AAGAAGCACA TCTCTTTGGA AGTCTGCATG CTACAGTTCT TCTATGCCTA TGTACAGAGG 
ATTCCTGTTT CCAGTTTGCT TGATAGCTGG CCGTCTCTGC TGGCGCTGCT GAAGGACTCG 
GTCCAGTTGG GTCTGCCTGC TCCTGGGCAG TTTCTCATAC TGGGAGTTCT GAATGAGTTT 
ATCTTGAAGA ATCCCAACCT GGAGAGTAAG AAAGACCAGC GAGAGCTGCA GGATGTGACT 
CACAAGGTGG TGGAGGCCAT CGGCACCATC GCTGGCTCCT CTTTGGAACA AACCACGTGG 
CTAAGGAGGA ACCTGGAGGT GAAGGCATCG CCTCAGATTG TTGTGGACGG AACCAACCTG 
GAGGCAGACG TAGAAGGTGC TCCGTTACCA AACTTAATGC TCACAGTGAT GGAGGCCTCC 
AGCTTCACTC CATCTGTGTA CAGTGTTCAC GCCCTCACGC TGCTGGCCGA GGTGCTGGCT 
CATCTATTGG ACATGGTGTT CTACAGTGAC GAGAAGGAGA AAGTCATCCC TCTGCTGGTT 
AACATTATGC ACTACGTAGT TCCCTACCTC CGCAACCACA GTGCTCACAA TGCCCCCAGC 
TACCGAGCCT GCATCCAGCT GCTGAGCAGC CTGAGCGGCT ACCAGTACAC CCGCCGTGCC 
TGGAAAAAAG AGGCCTTCGA CCTCTTCATG GACCACACCT TCTTCCAGAT GGATGCTTCG 
TGTGTCAGCC ACTGGAGAGC CATCATTGAC CACCTGATGA CTCATGACAA GACCACATTT 
AGAGACCTGA TGACCCGGGT GGCGGTGGCT CAGAGCAGCT CCCTGAGTTT GTTCACCAAC 
AGAGACGCTG AGCTGGAGCA GAGGGCCATG CTGCTCAAAC GCCTGGCCTT CACCATCTAC 
AGCAGCGAGG TGGACCAGTA CCAGAAGTAC CTGCCGGACA TCCAAGAGCG TCTGGTGGAG 
AGCCTGCGTC TGCCTCAGGT ACCCATCCTC CACGCTCAGG TGTTCCTGTT CTTCAGAGTG 
CTGCTGCTGC GCATGTCGCC TCAACACCTC ACGTCACTCT GGCCCACCAT GATCACTGAG 
CTGGTCCAAG TGTTTCTGCT GATGGAGCAG GAGTTAACCG CTGACGAGGA CATATCAAGG 
ACGTCAGGAC CGTCGGTTGT CGGGTTGGAG ACCACCTACT CTGGAGGGAA CGGCTTCTCC 
ACGTCCTACA ACAGCCAGCG CTGGCTCAAC CTCTACCTCT CTGCTTGCAA GTTCCTGGAT 
CTTGCCTTGG CGTTGCCTCC TGAGAGTCTC CCTCAGTTCC AGATGTACCG CTGGGCCTTC 
ATCCCAGAGG CTTCAGACGA TTCAGGGTTG GAAGTGAGAC GCCAGGGGAC GCATCAGAGG 
GAGTTCAAGC CCTACGTGGT CCGTCTGGTG AAGCTGCTGA GAAAACGAGC CAAGAAAAAC 
CCAGAGGAGG ACTGTTCCAC TAAAACCCTG CCGTGGGAGC CGGGCCACCT GATGCTGACC 
CTCTACGTCA TCCGCAGCAT GGAGCAGCTG CTGCCCTTCT TCAACCTGCT CAGCCAGGTC 
TTCAACAGCA GGGCGAGCTG CCGATCGGGG CCCGTCTACA CCCACAATCC CGTGGATTCC 
TCCTTCCCCG CCAGCAAGGA GGGCCACAAG CTGGAGAGCC AGAAGGTGTT CTGGAGCCGA 
GCCCGACAGA ACATCGAGGA GATGGTGGAA AAAGACTTTC TAGAGGGTCT TATCAAGACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOPEY1 ( XM_023330626.1 ) cDNA ORF clone, Xiphophorus maculatus(Southern platyfish) -> XP_023186394.1 Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X6, mRNA.

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CloneID	OXi35843
Clone ID Related Accession (Same CDS sequence)	XM_023330626.1
Accession Version	XM_023330626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	protein dopey-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036445.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAG AGGAAGTGGA GCTGCTCAGC GACTCAAAGT ACAGGAACTA TGTGGCAGCA 
GTGGACAAAG CCCTCAAGAA CTTTGAGTAT TCCAGCGAGT GGGCCGACCT CATCTCCGCA 
CTGGGCAAGC TCAACAAGGT TTTGCAGAAC AATGCAAAAT ATCAAGTTGT CCCCAAGAAG 
CTGACAATAG GCAAACGGCT GGCCCAGTGC CTCCACCCAG CCCTGCCCAG CGGCGTGCAC 
CGGAAGGCCT TGGAGACCTA CGAGATCATT TTCAAGATCA TTGGGCCCAA GAGGCTGGCC 
AAAGACCTCT TTCTGTACAG CTCGGGACTC TTCCCACTAC TCTCCAACGC TGCTATGTCT 
GTGAAGCCCG TGTTGCTGGG GCTCTATGAG ACCTACTACC TGCCCCTGGG GAAGACTCTC 
AAACCAGGCC TGCAGGGCTT ACTGACCGGC GTGCTGCCTG GTCTGGAGGA AGGTTCAGAG 
TATTACGACA GAACCAACAC GCTGTTGGAG AAAGTTGCTG CAGCCGTGGA GCAGTCGGCC 
TTCTACAGTG CCTTGTGGGG CAGCATCCTG ACCAGCCCCT CTGTGCGTCT CCCCGGGGTC 
TCCTTCGTTC TGCTGCACCT CAACCGGAAG CTCTCCATGG AGGACCAGCT CTACGTCATG 
GGCAGCGACA TTGAGCTTAT GGTGGAGGCT GTTAGTACCT CGGTGCAGGA CTCAAGCGTT 
TTGGTCCAGA GAAGCACTCT GGACCTCATC CTCTTCTGCT TCCCCTTCCA TATGAGCCAG 
GCTACCCGCC CTGACATGAT CCGGATCCTT TCAGCAGCTT TGCATGTGGT TCTGAGAAGA 
GACATGTCTC TGAACCGCAG ACTCTACGCC TGGCTGCTGG GCTTTGACAA TAATGGCGGG 
ATGTCAGGCC CCCGCAGTAC TCGGCAGAGC AACCCGGAGG AACACGCTTC CCACTACTTC 
AACAGCTTCT CCAAGGACAT GCTCGTCCAG GCAATGGTTG GGATTCTGCA GGGCAAGGCT 
CGAGGTGGGG AGGAGGAGAG CATCCTGATG CACGACCTCA AGCCATTTCG CATCCTGATC 
AGTCTGCTGG ACAAACCAGA ACTAGGGCCG GCCATTCTAG AGGACGTTCT GATCGAAGTG 
TTTCGGACTC TTTACACACA GTGCCGGACA GAGTTGGACC TTCAAAACCA GAGTCCGTTC 
AGCAAAGATC ACACACACCT CAGCAGTAAA CTGCGAGAAA ACAAGAAGAC GGCAGAGCTT 
ATCAAAACAG CCAATCTCCT GTTCAACTCG TTTGAGCCGT ACTACATGTG GGACTACATC 
GCTCGCTGGT TTGAGGAGTG CTGCAGGAGA ACATTGAACC GCAGCACACG TGTTGCACGA 
TACGGTTGGA GTCTGGATTC TCCTGATCTT TCTCTGGTGG AGTTCTGTCA GTTGGTAGAT 
TTTCTACTAG ACATTGTTTC ATTGCCTACT AGAAGCATGA GGGTAATCTG CCAGGAGACG 
TACATAGAAA TCCAGACGGA GCATCTCCCT CAACTGCTGT TGCGGATGGT GGCAGCTCTG 
ACCTGTCATC TCCAGGCGCT GGACCTCGGG GAGCTCACCC ACTGCCTTCG CCTCTGCTCC 
AAGATCCTCA GCAAGGTGCA GCCTCCGTTG GTGTCCCCGC TCGCCCTCCC CACTCCTCCC 
TCTGTCCCAG AGATGACCGA CAGTGGGGAC GTGTTTGACG ATGGGGAAAT CCCTACTGGC 
AGTCGCTCTG CAGAAAGTGG CTTCACAGAC TTTGTTCAAT ACCGAGAAGA TGGTTCCGAG 
GATCCAGAGC GAGCCTCTCA TCCCCACCCG CCTCTTAGAG CAGGCAGTCG TCCGTCAGGC 
TCGTGTCAGT CCAAGCCTTT GGACAAACCA GTCATGCAGT GTTGCCTGGA GCACTTCCAG 
CAGTTTCTCT CCTGTCTGAT CACTTTTTAT ATTGTTCCTG AGCACCTGGA CAAAGCTGAG 
GGTCAGAGAG GTGAGCTCTT GCATTCGGGG CCTTTGGTTT CCGCAGGTTC CCAGCCCAGT 
AATTATACGG AGCCGTGCTC AGGAGCTGGA GTGGTCCAGA AAGATTGTCT GGCTGCATTT 
ACTGCTGCCT GTCAGCTCTT CTTAGAATGC TCCAGTTTCC CAGTCTACAT TGCAGAGGGC 
AACCTCAAGT CCTCGCCTGC ACAGGAAGAG CAATGCGACA GTGAGCAGGT TCGGCTGCCA 
GTGTGGCTGC AGACCCTGAT GGATGCCTGC TGCCTGGCTG GTGACTTCAG CCTACAGGGT 
GTTGCCATCT CCCTGCTTAT GGACCTAATA GGCCTGACTC AGTCAGTTGC TGTGGTGACG 
GCAGAGAGTC TGGCTTCCAG TAGCAGTACG GAGCCCACTC AGCCAATGAG CCCCAGCCAG 
GGTCGGGTGG CTGTGGTCAT TAGGCCTCCA CTCACTCAGG GAATCCTGAA GTATATTGCG 
GATAAAACGG ACTTCTTCAA GAGCGTTGCA GTGATATTAT GGGACCAGCT GGGTGAAGGA 
ACTCCTCAGC ATCACCAGCG CAGTGTTGAG CTCTTTTATC AGCTACACAA CCTGGTCCCT 
TCATCCAGCA CCTGTGAGGA CGTCATAAGC CAGCAACTCA TGCACAGAGA CAAGAGAATT 
CGTTTGGCAG CCCACGTGAA GTTCTCTGTT CTGTGGCATT TAACGCGAGA CTTGAACATC 
ACCAAGTCTT CTCCGTTTAG TCGCACATTT GACAGGTCTC TCTTCATCAT GCTCGACAGC 
TTAAGTTATT GGGATCCATG TACCAGTGCG GTCGGTCGGG CTTGGTTAAA TCAAGTCCTT 
CAGAGACATG ACATTGCACG GGTTCTTGAG CCACTCCTAC TTCTTCTGCT CCATCCTAAA 
ACTCACAGGG TTTCTATTCA GAAGGTGCAA GCCCAACGCC ATTGGGCGCA GATATTTCCT 
GATCCACCTG AGCAGGAACC AACAGAACCA ATCTACATTA GAGACTCCGG CTTCTCAGAC 
AACTTTCTTC AGATTCGAAG CGAAGGGGGT GGGCATGAGG ACTTCAGAGG TCTGACAATA 
GGTGACATGG AGCCATTTTG CCTGACGGTA AACCCCCTAA GTGACAGCTT GTCCATTCTT 
AGCCTGAGCA GTGAAAACTT GCACTTAGCT GGTGAGTATC AGACCAGGGA CCAGCAGGAG 
GAGCAACAGA GTTCAAAATC AAGTGGTTCT CATTCATCTA CACTGGAAAA CGGTAGCTTT 
GAAGACCCTG AGGGGGTCAG CTACATCGGA AATGGCTCAG ATCGACCGCT TGGTTCATCT 
GATGAAACGC CTGACGACTC CATAGAGGAA GCTGTGTCTT GTGTAGTGAA GGACCTGATT 
GATAGGGTTC TGAATTTAGT TGATGAGGAA TCAAATGAAA CCTCATCTCC ACCTGAAAAT 
TGGCCCCAAA CTGATACTGA AAGCACTTCT TCAGACACCT CCACCGGGAT CCGCCTGGAC 
TCCTGTCCTC CTCAATGCTC CAACCACCAG AATCTGCCAC AGTTATTGGC TGAAGGCACA 
CTAGAGTTCC TCTCTGTTAC TCCAGCGGAG GGGCTACACA AAGAGGGCAT CGCCCGCCAC 
AGTTCATCGC CCTCCATCGT CATGTTGCCA GACAGCACTG ATGCAGCCAC TCCAGACCGC 
AGCCTGCAGG TGGAGGACCC TCAGGCCCGC AAACGTAGCC ACAGCAGCAC TCAGCTTAGC 
CTTAAAGGGA AGATCATGGA GAAGCTGGTA GACAAGTCTC CAGGGGCAAA GCCCAAGATC 
AAGAAGACTA AGAGGAAAGA GGAAGAGAAG CAGAAAAAAA CGAACCAAGC TGAGAAACTG 
CATCCACTCA GTATTTTCTT TGGAGACAGT CTAGACCTCG AGAACTGGTA CAGCTGTGGT 
GAAGGGGAGG TGTCGGAGAT CGAGAGTGAC ATGGGCTCAC CGAGCGGGGG CTCTTGCGGG 
ACCGGTGCTG GGGCGAACGT CACAGGGCGC AGATCGTCCT CCACTCTGCC TCGTTTTAAC 
ATCCATCCTC TCTACCAGCA CGTCCTGCTC TACCTACAGC TCTACGACTC CTCTAGGACC 
CTGCATGCCC TGTCGGCGAT CGCAGCCATG GTGAGAGCCG CTCCCTCAGG CTTTGTAAGC 
GCCATCTCCA CCACCAGCAT CAATAACACC TACACTCCAC AGCTCTCCTT GCTGCAAAAC 
CTACTTGCTC GCCACAGAGT CTCTGTTATG GGCAAAGACT TCTACTGCCC CATTCCCCAG 
GACTCTCACT CCCATTCTTT CCGCAGTGCC ATGTACCTGG AGATCATCAT CTCCCTCTGT 
CTCTACTTCC TGAGAAGCTA TTACTCTGCC CATGTGACAT TGGGTCCTCA GGACTTGGCA 
GGAAACCGGG CCATGCAGCT GACCAGCGTG GAGGTCTTGA CACTGCTATT CAGCGAACTA 
GCCAAAGTCA CAGCTGGCTC GGCAAAGGGT TTTGCTAGCT TCATCAGTGA CGTCCTTTCC 
AAGTGCAAAG TTCAGAAAGT CATCCTCCAT TGCCTGCTTT CCTCCATATT CAGCGCCCAG 
AAATGGCATG ACCAACGGAC GGCAGGGGTC AACGTGGCCA CGGTGGAGGA AGGTCTCTCG 
GAGGACAGCG TCATCAACAT GTCGGAGGAC CAGATAGACA GCTGCAGCGC CGTTCAGTCC 
CAGCTGCTCC GGCTGCTGCA GAGTCTAGTT GTACTTGAGC ATAGGGTCTT AATGCCAGCA 
GACGAAGGTG GAGAAGGAGG ACCTGCCGCA GGAGGAGCAG GTGGAGGGCC AGGAAGCGGT 
GGAGTGGCAG GATTTGAGAT CCTAGGAGGG GAGGTGGAGC ATGTTAACCC TCAGCAGCCT 
ATGACGTCAC TGCAGTACCT CCATGGTCAG CCTATTACAG CTCAGGGCAT GTTCCTGTGT 
GCAGTCATCA GGGCCCTGCA CCAGCACCAT GCCTGTAAGA TGCACCCCCA ATGGATAGGG 
CTGATCACAA CCACCCTGCC TTACATGGGG AGGGTCCTGA GGAGGGTGGT GGCCTCAGTG 
ACTCTGCAGC TATGCAGAAA CCTGGATAAT CTCCTCCAGC AGTACCGCTA TGAAACCAGC 
ATAACTGACA CCAGACCCCA GTGGATGGCT CTCTGCATCC CTCCAGACCT GATTCTGACT 
GTGCTGGAGG GAATCACTTC CATTATCCAT TACTGTCTGC TGGACCCCAC TTCACAGTAC 
CACCAGTTAC AAGTGAGTGT TGACCAGAAG CACCTGGCCG AGGCGCGTTC AGGTATCCTG 
TCCATCCTCC ACACCATCAT GTCTTCCGTC ACGCTGCTGT GGAGCGTGCT CCACCAAGCC 
GACAGCTCTG ACAAGCCAGC TGCCGCTTCA GCTGCCGCCA CCTCAAACAT CAACCTAGGC 
TCCACCAAGA ATCTCCGTCA GCAGATCTTG GAACTGCTGG GTCCAATCTC CATGAACCAC 
GGCGCTCATT TCATGGCTGC AATTGCTTAT GTTTGGAACG AGAGAAAACA AGTCAAGGCG 
CCAATCAGAA ATAAGGTCAT CCCTGTAGCG AGTGAGGAGC AGCTGCTGCT GGTTGAGCTC 
GTTCGGTCGG TCAGCGCCAT GCGCACTGAG ACTGTTATGC AGACAGTGAA GGAGGTCCTG 
AAGCAGCCAC CTGCTATCGC AAAAGACAAG AAGCACATCT CTTTGGAAGT CTGCATGCTA 
CAGTTCTTCT ATGCCTATGT ACAGAGGATT CCTGTTTCCA GTTTGCTTGA TAGCTGGCCG 
TCTCTGCTGG CGCTGCTGAA GGACTCGGTC CAGTTGGGTC TGCCTGCTCC TGGGCAGTTT 
CTCATACTGG GAGTTCTGAA TGAGTTTATC TTGAAGAATC CCAACCTGGA GAGTAAGAAA 
GACCAGCGAG AGCTGCAGGA TGTGACTCAC AAGGTGGTGG AGGCCATCGG CACCATCGCT 
GGCTCCTCTT TGGAACAAAC CACGTGGCTA AGGAGGAACC TGGAGGTGAA GGCATCGCCT 
CAGATTGTTG TGGACGGAAC CAACCTGGAG GCAGACGTAG AAGGTGCTCC GTTACCAAAC 
TTAATGCTCA CAGTGATGGA GGCCTCCAGC TTCACTCCAT CTGTGTACAG TGTTCACGCC 
CTCACGCTGC TGGCCGAGGT GCTGGCTCAT CTATTGGACA TGGTGTTCTA CAGTGACGAG 
AAGGAGAAAG TCATCCCTCT GCTGGTTAAC ATTATGCACT ACGTAGTTCC CTACCTCCGC 
AACCACAGTG CTCACAATGC CCCCAGCTAC CGAGCCTGCA TCCAGCTGCT GAGCAGCCTG 
AGCGGCTACC AGTACACCCG CCGTGCCTGG AAAAAAGAGG CCTTCGACCT CTTCATGGAC 
CACACCTTCT TCCAGATGGA TGCTTCGTGT GTCAGCCACT GGAGAGCCAT CATTGACCAC 
CTGATGACTC ATGACAAGAC CACATTTAGA GACCTGATGA CCCGGGTGGC GGTGGCTCAG 
AGCAGCTCCC TGAGTTTGTT CACCAACAGA GACGCTGAGC TGGAGCAGAG GGCCATGCTG 
CTCAAACGCC TGGCCTTCAC CATCTACAGC AGCGAGGTGG ACCAGTACCA GAAGTACCTG 
CCGGACATCC AAGAGCGTCT GGTGGAGAGC CTGCGTCTGC CTCAGGTACC CATCCTCCAC 
GCTCAGGTGT TCCTGTTCTT CAGAGTGCTG CTGCTGCGCA TGTCGCCTCA ACACCTCACG 
TCACTCTGGC CCACCATGAT CACTGAGCTG GTCCAAGTGT TTCTGCTGAT GGAGCAGGAG 
TTAACCGCTG ACGAGGACAT ATCAAGGACG TCAGGACCGT CGGTTGTCGG GTTGGAGACC 
ACCTACTCTG GAGGGAACGG CTTCTCCACG TCCTACAACA GCCAGCGCTG GCTCAACCTC 
TACCTCTCTG CTTGCAAGTT CCTGGATCTT GCCTTGGCGT TGCCTCCTGA GAGTCTCCCT 
CAGTTCCAGA TGTACCGCTG GGCCTTCATC CCAGAGGCTT CAGACGATTC AGGGTTGGAA 
GTGAGACGCC AGGGGACGCA TCAGAGGGAG TTCAAGCCCT ACGTGGTCCG TCTGGTGAAG 
CTGCTGAGAA AACGAGCCAA GAAAAACCCA GAGGAGGACT GTTCCACTAA AACCCTGCCG 
TGGGAGCCGG GCCACCTGAT GCTGACCCTC TACGTCATCC GCAGCATGGA GCAGCTGCTG 
CCCTTCTTCA ACCTGCTCAG CCAGGTCTTC AACAGCAGGG CGAGCTGCCG ATCGGGGCCC 
GTCTACACCC ACAATCCCGT GGATTCCTCC TTCCCCGCCA GCAAGGAGGG CCACAAGCTG 
GAGAGCCAGA AGGTGTTCTG GAGCCGAGCC CGACAGAACA TCGAGGAGAT GGTGGAAAAA 
GACTTTCTAG AGGGTCTTAT CAAGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023186394.1
CDS	395..7804
Translation	MNAEEVELLSDSKYRNYVAAVDKALKNFEYSSEWADLISALGKLNKVLQNNAKYQVVPKKLTIGKRLAQCLHPALPSGVHRKALETYEIIFKIIGPKRLAKDLFLYSSGLFPLLSNAAMSVKPVLLGLYETYYLPLGKTLKPGLQGLLTGVLPGLEEGSEYYDRTNTLLEKVAAAVEQSAFYSALWGSILTSPSVRLPGVSFVLLHLNRKLSMEDQLYVMGSDIELMVEAVSTSVQDSSVLVQRSTLDLILFCFPFHMSQATRPDMIRILSAALHVVLRRDMSLNRRLYAWLLGFDNNGGMSGPRSTRQSNPEEHASHYFNSFSKDMLVQAMVGILQGKARGGEEESILMHDLKPFRILISLLDKPELGPAILEDVLIEVFRTLYTQCRTELDLQNQSPFSKDHTHLSSKLRENKKTAELIKTANLLFNSFEPYYMWDYIARWFEECCRRTLNRSTRVARYGWSLDSPDLSLVEFCQLVDFLLDIVSLPTRSMRVICQETYIEIQTEHLPQLLLRMVAALTCHLQALDLGELTHCLRLCSKILSKVQPPLVSPLALPTPPSVPEMTDSGDVFDDGEIPTGSRSAESGFTDFVQYREDGSEDPERASHPHPPLRAGSRPSGSCQSKPLDKPVMQCCLEHFQQFLSCLITFYIVPEHLDKAEGQRGELLHSGPLVSAGSQPSNYTEPCSGAGVVQKDCLAAFTAACQLFLECSSFPVYIAEGNLKSSPAQEEQCDSEQVRLPVWLQTLMDACCLAGDFSLQGVAISLLMDLIGLTQSVAVVTAESLASSSSTEPTQPMSPSQGRVAVVIRPPLTQGILKYIADKTDFFKSVAVILWDQLGEGTPQHHQRSVELFYQLHNLVPSSSTCEDVISQQLMHRDKRIRLAAHVKFSVLWHLTRDLNITKSSPFSRTFDRSLFIMLDSLSYWDPCTSAVGRAWLNQVLQRHDIARVLEPLLLLLLHPKTHRVSIQKVQAQRHWAQIFPDPPEQEPTEPIYIRDSGFSDNFLQIRSEGGGHEDFRGLTIGDMEPFCLTVNPLSDSLSILSLSSENLHLAGEYQTRDQQEEQQSSKSSGSHSSTLENGSFEDPEGVSYIGNGSDRPLGSSDETPDDSIEEAVSCVVKDLIDRVLNLVDEESNETSSPPENWPQTDTESTSSDTSTGIRLDSCPPQCSNHQNLPQLLAEGTLEFLSVTPAEGLHKEGIARHSSSPSIVMLPDSTDAATPDRSLQVEDPQARKRSHSSTQLSLKGKIMEKLVDKSPGAKPKIKKTKRKEEEKQKKTNQAEKLHPLSIFFGDSLDLENWYSCGEGEVSEIESDMGSPSGGSCGTGAGANVTGRRSSSTLPRFNIHPLYQHVLLYLQLYDSSRTLHALSAIAAMVRAAPSGFVSAISTTSINNTYTPQLSLLQNLLARHRVSVMGKDFYCPIPQDSHSHSFRSAMYLEIIISLCLYFLRSYYSAHVTLGPQDLAGNRAMQLTSVEVLTLLFSELAKVTAGSAKGFASFISDVLSKCKVQKVILHCLLSSIFSAQKWHDQRTAGVNVATVEEGLSEDSVINMSEDQIDSCSAVQSQLLRLLQSLVVLEHRVLMPADEGGEGGPAAGGAGGGPGSGGVAGFEILGGEVEHVNPQQPMTSLQYLHGQPITAQGMFLCAVIRALHQHHACKMHPQWIGLITTTLPYMGRVLRRVVASVTLQLCRNLDNLLQQYRYETSITDTRPQWMALCIPPDLILTVLEGITSIIHYCLLDPTSQYHQLQVSVDQKHLAEARSGILSILHTIMSSVTLLWSVLHQADSSDKPAAASAAATSNINLGSTKNLRQQILELLGPISMNHGAHFMAAIAYVWNERKQVKAPIRNKVIPVASEEQLLLVELVRSVSAMRTETVMQTVKEVLKQPPAIAKDKKHISLEVCMLQFFYAYVQRIPVSSLLDSWPSLLALLKDSVQLGLPAPGQFLILGVLNEFILKNPNLESKKDQRELQDVTHKVVEAIGTIAGSSLEQTTWLRRNLEVKASPQIVVDGTNLEADVEGAPLPNLMLTVMEASSFTPSVYSVHALTLLAEVLAHLLDMVFYSDEKEKVIPLLVNIMHYVVPYLRNHSAHNAPSYRACIQLLSSLSGYQYTRRAWKKEAFDLFMDHTFFQMDASCVSHWRAIIDHLMTHDKTTFRDLMTRVAVAQSSSLSLFTNRDAELEQRAMLLKRLAFTIYSSEVDQYQKYLPDIQERLVESLRLPQVPILHAQVFLFFRVLLLRMSPQHLTSLWPTMITELVQVFLLMEQELTADEDISRTSGPSVVGLETTYSGGNGFSTSYNSQRWLNLYLSACKFLDLALALPPESLPQFQMYRWAFIPEASDDSGLEVRRQGTHQREFKPYVVRLVKLLRKRAKKNPEEDCSTKTLPWEPGHLMLTLYVIRSMEQLLPFFNLLSQVFNSRASCRSGPVYTHNPVDSSFPASKEGHKLESQKVFWSRARQNIEEMVEKDFLEGLIKT

Target ORF information:

RefSeq Version	XM_023330626.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023330626.1

ORF Insert Sequence:

ATGAATGCAG AGGAAGTGGA GCTGCTCAGC GACTCAAAGT ACAGGAACTA TGTGGCAGCA 
GTGGACAAAG CCCTCAAGAA CTTTGAGTAT TCCAGCGAGT GGGCCGACCT CATCTCCGCA 
CTGGGCAAGC TCAACAAGGT TTTGCAGAAC AATGCAAAAT ATCAAGTTGT CCCCAAGAAG 
CTGACAATAG GCAAACGGCT GGCCCAGTGC CTCCACCCAG CCCTGCCCAG CGGCGTGCAC 
CGGAAGGCCT TGGAGACCTA CGAGATCATT TTCAAGATCA TTGGGCCCAA GAGGCTGGCC 
AAAGACCTCT TTCTGTACAG CTCGGGACTC TTCCCACTAC TCTCCAACGC TGCTATGTCT 
GTGAAGCCCG TGTTGCTGGG GCTCTATGAG ACCTACTACC TGCCCCTGGG GAAGACTCTC 
AAACCAGGCC TGCAGGGCTT ACTGACCGGC GTGCTGCCTG GTCTGGAGGA AGGTTCAGAG 
TATTACGACA GAACCAACAC GCTGTTGGAG AAAGTTGCTG CAGCCGTGGA GCAGTCGGCC 
TTCTACAGTG CCTTGTGGGG CAGCATCCTG ACCAGCCCCT CTGTGCGTCT CCCCGGGGTC 
TCCTTCGTTC TGCTGCACCT CAACCGGAAG CTCTCCATGG AGGACCAGCT CTACGTCATG 
GGCAGCGACA TTGAGCTTAT GGTGGAGGCT GTTAGTACCT CGGTGCAGGA CTCAAGCGTT 
TTGGTCCAGA GAAGCACTCT GGACCTCATC CTCTTCTGCT TCCCCTTCCA TATGAGCCAG 
GCTACCCGCC CTGACATGAT CCGGATCCTT TCAGCAGCTT TGCATGTGGT TCTGAGAAGA 
GACATGTCTC TGAACCGCAG ACTCTACGCC TGGCTGCTGG GCTTTGACAA TAATGGCGGG 
ATGTCAGGCC CCCGCAGTAC TCGGCAGAGC AACCCGGAGG AACACGCTTC CCACTACTTC 
AACAGCTTCT CCAAGGACAT GCTCGTCCAG GCAATGGTTG GGATTCTGCA GGGCAAGGCT 
CGAGGTGGGG AGGAGGAGAG CATCCTGATG CACGACCTCA AGCCATTTCG CATCCTGATC 
AGTCTGCTGG ACAAACCAGA ACTAGGGCCG GCCATTCTAG AGGACGTTCT GATCGAAGTG 
TTTCGGACTC TTTACACACA GTGCCGGACA GAGTTGGACC TTCAAAACCA GAGTCCGTTC 
AGCAAAGATC ACACACACCT CAGCAGTAAA CTGCGAGAAA ACAAGAAGAC GGCAGAGCTT 
ATCAAAACAG CCAATCTCCT GTTCAACTCG TTTGAGCCGT ACTACATGTG GGACTACATC 
GCTCGCTGGT TTGAGGAGTG CTGCAGGAGA ACATTGAACC GCAGCACACG TGTTGCACGA 
TACGGTTGGA GTCTGGATTC TCCTGATCTT TCTCTGGTGG AGTTCTGTCA GTTGGTAGAT 
TTTCTACTAG ACATTGTTTC ATTGCCTACT AGAAGCATGA GGGTAATCTG CCAGGAGACG 
TACATAGAAA TCCAGACGGA GCATCTCCCT CAACTGCTGT TGCGGATGGT GGCAGCTCTG 
ACCTGTCATC TCCAGGCGCT GGACCTCGGG GAGCTCACCC ACTGCCTTCG CCTCTGCTCC 
AAGATCCTCA GCAAGGTGCA GCCTCCGTTG GTGTCCCCGC TCGCCCTCCC CACTCCTCCC 
TCTGTCCCAG AGATGACCGA CAGTGGGGAC GTGTTTGACG ATGGGGAAAT CCCTACTGGC 
AGTCGCTCTG CAGAAAGTGG CTTCACAGAC TTTGTTCAAT ACCGAGAAGA TGGTTCCGAG 
GATCCAGAGC GAGCCTCTCA TCCCCACCCG CCTCTTAGAG CAGGCAGTCG TCCGTCAGGC 
TCGTGTCAGT CCAAGCCTTT GGACAAACCA GTCATGCAGT GTTGCCTGGA GCACTTCCAG 
CAGTTTCTCT CCTGTCTGAT CACTTTTTAT ATTGTTCCTG AGCACCTGGA CAAAGCTGAG 
GGTCAGAGAG GTGAGCTCTT GCATTCGGGG CCTTTGGTTT CCGCAGGTTC CCAGCCCAGT 
AATTATACGG AGCCGTGCTC AGGAGCTGGA GTGGTCCAGA AAGATTGTCT GGCTGCATTT 
ACTGCTGCCT GTCAGCTCTT CTTAGAATGC TCCAGTTTCC CAGTCTACAT TGCAGAGGGC 
AACCTCAAGT CCTCGCCTGC ACAGGAAGAG CAATGCGACA GTGAGCAGGT TCGGCTGCCA 
GTGTGGCTGC AGACCCTGAT GGATGCCTGC TGCCTGGCTG GTGACTTCAG CCTACAGGGT 
GTTGCCATCT CCCTGCTTAT GGACCTAATA GGCCTGACTC AGTCAGTTGC TGTGGTGACG 
GCAGAGAGTC TGGCTTCCAG TAGCAGTACG GAGCCCACTC AGCCAATGAG CCCCAGCCAG 
GGTCGGGTGG CTGTGGTCAT TAGGCCTCCA CTCACTCAGG GAATCCTGAA GTATATTGCG 
GATAAAACGG ACTTCTTCAA GAGCGTTGCA GTGATATTAT GGGACCAGCT GGGTGAAGGA 
ACTCCTCAGC ATCACCAGCG CAGTGTTGAG CTCTTTTATC AGCTACACAA CCTGGTCCCT 
TCATCCAGCA CCTGTGAGGA CGTCATAAGC CAGCAACTCA TGCACAGAGA CAAGAGAATT 
CGTTTGGCAG CCCACGTGAA GTTCTCTGTT CTGTGGCATT TAACGCGAGA CTTGAACATC 
ACCAAGTCTT CTCCGTTTAG TCGCACATTT GACAGGTCTC TCTTCATCAT GCTCGACAGC 
TTAAGTTATT GGGATCCATG TACCAGTGCG GTCGGTCGGG CTTGGTTAAA TCAAGTCCTT 
CAGAGACATG ACATTGCACG GGTTCTTGAG CCACTCCTAC TTCTTCTGCT CCATCCTAAA 
ACTCACAGGG TTTCTATTCA GAAGGTGCAA GCCCAACGCC ATTGGGCGCA GATATTTCCT 
GATCCACCTG AGCAGGAACC AACAGAACCA ATCTACATTA GAGACTCCGG CTTCTCAGAC 
AACTTTCTTC AGATTCGAAG CGAAGGGGGT GGGCATGAGG ACTTCAGAGG TCTGACAATA 
GGTGACATGG AGCCATTTTG CCTGACGGTA AACCCCCTAA GTGACAGCTT GTCCATTCTT 
AGCCTGAGCA GTGAAAACTT GCACTTAGCT GGTGAGTATC AGACCAGGGA CCAGCAGGAG 
GAGCAACAGA GTTCAAAATC AAGTGGTTCT CATTCATCTA CACTGGAAAA CGGTAGCTTT 
GAAGACCCTG AGGGGGTCAG CTACATCGGA AATGGCTCAG ATCGACCGCT TGGTTCATCT 
GATGAAACGC CTGACGACTC CATAGAGGAA GCTGTGTCTT GTGTAGTGAA GGACCTGATT 
GATAGGGTTC TGAATTTAGT TGATGAGGAA TCAAATGAAA CCTCATCTCC ACCTGAAAAT 
TGGCCCCAAA CTGATACTGA AAGCACTTCT TCAGACACCT CCACCGGGAT CCGCCTGGAC 
TCCTGTCCTC CTCAATGCTC CAACCACCAG AATCTGCCAC AGTTATTGGC TGAAGGCACA 
CTAGAGTTCC TCTCTGTTAC TCCAGCGGAG GGGCTACACA AAGAGGGCAT CGCCCGCCAC 
AGTTCATCGC CCTCCATCGT CATGTTGCCA GACAGCACTG ATGCAGCCAC TCCAGACCGC 
AGCCTGCAGG TGGAGGACCC TCAGGCCCGC AAACGTAGCC ACAGCAGCAC TCAGCTTAGC 
CTTAAAGGGA AGATCATGGA GAAGCTGGTA GACAAGTCTC CAGGGGCAAA GCCCAAGATC 
AAGAAGACTA AGAGGAAAGA GGAAGAGAAG CAGAAAAAAA CGAACCAAGC TGAGAAACTG 
CATCCACTCA GTATTTTCTT TGGAGACAGT CTAGACCTCG AGAACTGGTA CAGCTGTGGT 
GAAGGGGAGG TGTCGGAGAT CGAGAGTGAC ATGGGCTCAC CGAGCGGGGG CTCTTGCGGG 
ACCGGTGCTG GGGCGAACGT CACAGGGCGC AGATCGTCCT CCACTCTGCC TCGTTTTAAC 
ATCCATCCTC TCTACCAGCA CGTCCTGCTC TACCTACAGC TCTACGACTC CTCTAGGACC 
CTGCATGCCC TGTCGGCGAT CGCAGCCATG GTGAGAGCCG CTCCCTCAGG CTTTGTAAGC 
GCCATCTCCA CCACCAGCAT CAATAACACC TACACTCCAC AGCTCTCCTT GCTGCAAAAC 
CTACTTGCTC GCCACAGAGT CTCTGTTATG GGCAAAGACT TCTACTGCCC CATTCCCCAG 
GACTCTCACT CCCATTCTTT CCGCAGTGCC ATGTACCTGG AGATCATCAT CTCCCTCTGT 
CTCTACTTCC TGAGAAGCTA TTACTCTGCC CATGTGACAT TGGGTCCTCA GGACTTGGCA 
GGAAACCGGG CCATGCAGCT GACCAGCGTG GAGGTCTTGA CACTGCTATT CAGCGAACTA 
GCCAAAGTCA CAGCTGGCTC GGCAAAGGGT TTTGCTAGCT TCATCAGTGA CGTCCTTTCC 
AAGTGCAAAG TTCAGAAAGT CATCCTCCAT TGCCTGCTTT CCTCCATATT CAGCGCCCAG 
AAATGGCATG ACCAACGGAC GGCAGGGGTC AACGTGGCCA CGGTGGAGGA AGGTCTCTCG 
GAGGACAGCG TCATCAACAT GTCGGAGGAC CAGATAGACA GCTGCAGCGC CGTTCAGTCC 
CAGCTGCTCC GGCTGCTGCA GAGTCTAGTT GTACTTGAGC ATAGGGTCTT AATGCCAGCA 
GACGAAGGTG GAGAAGGAGG ACCTGCCGCA GGAGGAGCAG GTGGAGGGCC AGGAAGCGGT 
GGAGTGGCAG GATTTGAGAT CCTAGGAGGG GAGGTGGAGC ATGTTAACCC TCAGCAGCCT 
ATGACGTCAC TGCAGTACCT CCATGGTCAG CCTATTACAG CTCAGGGCAT GTTCCTGTGT 
GCAGTCATCA GGGCCCTGCA CCAGCACCAT GCCTGTAAGA TGCACCCCCA ATGGATAGGG 
CTGATCACAA CCACCCTGCC TTACATGGGG AGGGTCCTGA GGAGGGTGGT GGCCTCAGTG 
ACTCTGCAGC TATGCAGAAA CCTGGATAAT CTCCTCCAGC AGTACCGCTA TGAAACCAGC 
ATAACTGACA CCAGACCCCA GTGGATGGCT CTCTGCATCC CTCCAGACCT GATTCTGACT 
GTGCTGGAGG GAATCACTTC CATTATCCAT TACTGTCTGC TGGACCCCAC TTCACAGTAC 
CACCAGTTAC AAGTGAGTGT TGACCAGAAG CACCTGGCCG AGGCGCGTTC AGGTATCCTG 
TCCATCCTCC ACACCATCAT GTCTTCCGTC ACGCTGCTGT GGAGCGTGCT CCACCAAGCC 
GACAGCTCTG ACAAGCCAGC TGCCGCTTCA GCTGCCGCCA CCTCAAACAT CAACCTAGGC 
TCCACCAAGA ATCTCCGTCA GCAGATCTTG GAACTGCTGG GTCCAATCTC CATGAACCAC 
GGCGCTCATT TCATGGCTGC AATTGCTTAT GTTTGGAACG AGAGAAAACA AGTCAAGGCG 
CCAATCAGAA ATAAGGTCAT CCCTGTAGCG AGTGAGGAGC AGCTGCTGCT GGTTGAGCTC 
GTTCGGTCGG TCAGCGCCAT GCGCACTGAG ACTGTTATGC AGACAGTGAA GGAGGTCCTG 
AAGCAGCCAC CTGCTATCGC AAAAGACAAG AAGCACATCT CTTTGGAAGT CTGCATGCTA 
CAGTTCTTCT ATGCCTATGT ACAGAGGATT CCTGTTTCCA GTTTGCTTGA TAGCTGGCCG 
TCTCTGCTGG CGCTGCTGAA GGACTCGGTC CAGTTGGGTC TGCCTGCTCC TGGGCAGTTT 
CTCATACTGG GAGTTCTGAA TGAGTTTATC TTGAAGAATC CCAACCTGGA GAGTAAGAAA 
GACCAGCGAG AGCTGCAGGA TGTGACTCAC AAGGTGGTGG AGGCCATCGG CACCATCGCT 
GGCTCCTCTT TGGAACAAAC CACGTGGCTA AGGAGGAACC TGGAGGTGAA GGCATCGCCT 
CAGATTGTTG TGGACGGAAC CAACCTGGAG GCAGACGTAG AAGGTGCTCC GTTACCAAAC 
TTAATGCTCA CAGTGATGGA GGCCTCCAGC TTCACTCCAT CTGTGTACAG TGTTCACGCC 
CTCACGCTGC TGGCCGAGGT GCTGGCTCAT CTATTGGACA TGGTGTTCTA CAGTGACGAG 
AAGGAGAAAG TCATCCCTCT GCTGGTTAAC ATTATGCACT ACGTAGTTCC CTACCTCCGC 
AACCACAGTG CTCACAATGC CCCCAGCTAC CGAGCCTGCA TCCAGCTGCT GAGCAGCCTG 
AGCGGCTACC AGTACACCCG CCGTGCCTGG AAAAAAGAGG CCTTCGACCT CTTCATGGAC 
CACACCTTCT TCCAGATGGA TGCTTCGTGT GTCAGCCACT GGAGAGCCAT CATTGACCAC 
CTGATGACTC ATGACAAGAC CACATTTAGA GACCTGATGA CCCGGGTGGC GGTGGCTCAG 
AGCAGCTCCC TGAGTTTGTT CACCAACAGA GACGCTGAGC TGGAGCAGAG GGCCATGCTG 
CTCAAACGCC TGGCCTTCAC CATCTACAGC AGCGAGGTGG ACCAGTACCA GAAGTACCTG 
CCGGACATCC AAGAGCGTCT GGTGGAGAGC CTGCGTCTGC CTCAGGTACC CATCCTCCAC 
GCTCAGGTGT TCCTGTTCTT CAGAGTGCTG CTGCTGCGCA TGTCGCCTCA ACACCTCACG 
TCACTCTGGC CCACCATGAT CACTGAGCTG GTCCAAGTGT TTCTGCTGAT GGAGCAGGAG 
TTAACCGCTG ACGAGGACAT ATCAAGGACG TCAGGACCGT CGGTTGTCGG GTTGGAGACC 
ACCTACTCTG GAGGGAACGG CTTCTCCACG TCCTACAACA GCCAGCGCTG GCTCAACCTC 
TACCTCTCTG CTTGCAAGTT CCTGGATCTT GCCTTGGCGT TGCCTCCTGA GAGTCTCCCT 
CAGTTCCAGA TGTACCGCTG GGCCTTCATC CCAGAGGCTT CAGACGATTC AGGGTTGGAA 
GTGAGACGCC AGGGGACGCA TCAGAGGGAG TTCAAGCCCT ACGTGGTCCG TCTGGTGAAG 
CTGCTGAGAA AACGAGCCAA GAAAAACCCA GAGGAGGACT GTTCCACTAA AACCCTGCCG 
TGGGAGCCGG GCCACCTGAT GCTGACCCTC TACGTCATCC GCAGCATGGA GCAGCTGCTG 
CCCTTCTTCA ACCTGCTCAG CCAGGTCTTC AACAGCAGGG CGAGCTGCCG ATCGGGGCCC 
GTCTACACCC ACAATCCCGT GGATTCCTCC TTCCCCGCCA GCAAGGAGGG CCACAAGCTG 
GAGAGCCAGA AGGTGTTCTG GAGCCGAGCC CGACAGAACA TCGAGGAGAT GGTGGAAAAA 
GACTTTCTAG AGGGTCTTAT CAAGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOPEY1 ( XM_023330627.1 ) cDNA ORF clone, Xiphophorus maculatus(Southern platyfish) -> XP_023186395.1 Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X7, mRNA.

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CloneID	OXi35844
Clone ID Related Accession (Same CDS sequence)	XM_023330627.1
Accession Version	XM_023330627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	protein dopey-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036445.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGCAG AGGAAGTGGA GCTGCTCAGC GACTCAAAGT ACAGGAACTA TGTGGCAGCA 
GTGGACAAAG CCCTCAAGAA CTTTGAGTAT TCCAGCGAGT GGGCCGACCT CATCTCCGCA 
CTGGGCAAGC TCAACAAGGT TTTGCAGAAC AATGCAAAAT ATCAAGTTGT CCCCAAGAAG 
CTGACAATAG GCAAACGGCT GGCCCAGTGC CTCCACCCAG CCCTGCCCAG CGGCGTGCAC 
CGGAAGGCCT TGGAGACCTA CGAGATCATT TTCAAGATCA TTGGGCCCAA GAGGCTGGCC 
AAAGACCTCT TTCTGTACAG CTCGGGACTC TTCCCACTAC TCTCCAACGC TGCTATGTCT 
GTGAAGCCCG TGTTGCTGGG GCTCTATGAG ACCTACTACC TGCCCCTGGG GAAGACTCTC 
AAACCAGGCC TGCAGGGCTT ACTGACCGGC GTGCTGCCTG GTCTGGAGGA AGGTTCAGAG 
TATTACGACA GAACCAACAC GCTGTTGGAG AAAGTTGCTG CAGCCGTGGA GCAGTCGGCC 
TTCTACAGTG CCTTGTGGGG CAGCATCCTG ACCAGCCCCT CTGTGCGTCT CCCCGGGGTC 
TCCTTCGTTC TGCTGCACCT CAACCGGAAG CTCTCCATGG AGGACCAGCT CTACGTCATG 
GGCAGCGACA TTGAGCTTAT GGTGGAGGCT GTTAGTACCT CGGTGCAGGA CTCAAGCGTT 
TTGGTCCAGA GAAGCACTCT GGACCTCATC CTCTTCTGCT TCCCCTTCCA TATGAGCCAG 
GCTACCCGCC CTGACATGAT CCGGATCCTT TCAGCAGCTT TGCATGTGGT TCTGAGAAGA 
GACATGTCTC TGAACCGCAG ACTCTACGCC TGGCTGCTGG GCCCCCGCAG TACTCGGCAG 
AGCAACCCGG AGGAACACGC TTCCCACTAC TTCAACAGCT TCTCCAAGGA CATGCTCGTC 
CAGGCAATGG TTGGGATTCT GCAGGGCAAG GCTCGAGGTG GGGAGGAGGA GAGCATCCTG 
ATGCACGACC TCAAGCCATT TCGCATCCTG ATCAGTCTGC TGGACAAACC AGAACTAGGG 
CCGGCCATTC TAGAGGACGT TCTGATCGAA GTGTTTCGGA CTCTTTACAC ACAGTGCCGG 
ACAGAGTTGG ACCTTCAAAA CCAGAGTCCG TTCAGCAAAG ATCACACACA CCTCAGCAGT 
AAACTGCGAG AAAACAAGAA GACGGCAGAG CTTATCAAAA CAGCCAATCT CCTGTTCAAC 
TCGTTTGAGC CGTACTACAT GTGGGACTAC ATCGCTCGCT GGTTTGAGGA GTGCTGCAGG 
AGAACATTGA ACCGCAGCAC ACGTGTTGCA CGATACGGTT GGAGTCTGGA TTCTCCTGAT 
CTTTCTCTGG TGGAGTTCTG TCAGTTGGTA GATTTTCTAC TAGACATTGT TTCATTGCCT 
ACTAGAAGCA TGAGGGTAAT CTGCCAGGAG ACGTACATAG AAATCCAGAC GGAGCATCTC 
CCTCAACTGC TGTTGCGGAT GGTGGCAGCT CTGACCTGTC ATCTCCAGGC GCTGGACCTC 
GGGGAGCTCA CCCACTGCCT TCGCCTCTGC TCCAAGATCC TCAGCAAGGT GCAGCCTCCG 
TTGGTGTCCC CGCTCGCCCT CCCCGTGAGT CCACAGCCGC CGGGCCTGGC GGCACAGAAC 
AAAAGCAGAG ATGTGGATGT TAAACAGACT CCTCCCTCTG TCCCAGAGAT GACCGACAGT 
GGGGACGTGT TTGACGATGG GGAAATCCCT ACTGGCAGTC GCTCTGCAGA AAGTGGCTTC 
ACAGACTTTG TTCAATACCG AGAAGATGGT TCCGAGGATC CAGAGCGAGC CTCTCATCCC 
CACCCGCCTC TTAGAGCAGG CAGTCGTCCG TCAGGCTCGT GTCAGTCCAA GCCTTTGGAC 
AAACCAGTCA TGCAGTGTTG CCTGGAGCAC TTCCAGCAGT TTCTCTCCTG TCTGATCACT 
TTTTATATTG TTCCTGAGCA CCTGGACAAA GCTGAGGGTC AGAGAGGTGA GCTCTTGCAT 
TCGGGGCCTT TGGTTTCCGC AGGTTCCCAG CCCAGTAATT ATACGGAGCC GTGCTCAGGA 
GCTGGAGTGG TCCAGAAAGA TTGTCTGGCT GCATTTACTG CTGCCTGTCA GCTCTTCTTA 
GAATGCTCCA GTTTCCCAGT CTACATTGCA GAGGGCAACC TCAAGTCCTC GCCTGCACAG 
GAAGAGCAAT GCGACAGTGA GCAGGTTCGG CTGCCAGTGT GGCTGCAGAC CCTGATGGAT 
GCCTGCTGCC TGGCTGGTGA CTTCAGCCTA CAGGGTGTTG CCATCTCCCT GCTTATGGAC 
CTAATAGGCC TGACTCAGTC AGTTGCTGTG GTGACGGCAG AGAGTCTGGC TTCCAGTAGC 
AGTACGGAGC CCACTCAGCC AATGAGCCCC AGCCAGGGTC GGGTGGCTGT GGTCATTAGG 
CCTCCACTCA CTCAGGGAAT CCTGAAGTAT ATTGCGGATA AAACGGACTT CTTCAAGAGC 
GTTGCAGTGA TATTATGGGA CCAGCTGGGT GAAGGAACTC CTCAGCATCA CCAGCGCAGT 
GTTGAGCTCT TTTATCAGCT ACACAACCTG GTCCCTTCAT CCAGCACCTG TGAGGACGTC 
ATAAGCCAGC AACTCATGCA CAGAGACAAG AGAATTCGTT TGGCAGCCCA CGTGAAGTTC 
TCTGTTCTGT GGCATTTAAC GCGAGACTTG AACATCACCA AGTCTTCTCC GTTTAGTCGC 
ACATTTGACA GGTCTCTCTT CATCATGCTC GACAGCTTAA GTTATTGGGA TCCATGTACC 
AGTGCGGTCG GTCGGGCTTG GTTAAATCAA GTCCTTCAGA GACATGACAT TGCACGGGTT 
CTTGAGCCAC TCCTACTTCT TCTGCTCCAT CCTAAAACTC ACAGGGTTTC TATTCAGAAG 
GTGCAAGCCC AACGCCATTG GGCGCAGATA TTTCCTGATC CACCTGAGCA GGAACCAACA 
GAACCAATCT ACATTAGAGA CTCCGGCTTC TCAGACAACT TTCTTCAGAT TCGAAGCGAA 
GGGGGTGGGC ATGAGGACTT CAGAGGTCTG ACAATAGGTG ACATGGAGCC ATTTTGCCTG 
ACGGTAAACC CCCTAAGTGA CAGCTTGTCC ATTCTTAGCC TGAGCAGTGA AAACTTGCAC 
TTAGCTGGTG AGTATCAGAC CAGGGACCAG CAGGAGGAGC AACAGAGTTC AAAATCAAGT 
GGTTCTCATT CATCTACACT GGAAAACGGT AGCTTTGAAG ACCCTGAGGG GGTCAGCTAC 
ATCGGAAATG GCTCAGATCG ACCGCTTGGT TCATCTGATG AAACGCCTGA CGACTCCATA 
GAGGAAGCTG TGTCTTGTGT AGTGAAGGAC CTGATTGATA GGGTTCTGAA TTTAGTTGAT 
GAGGAATCAA ATGAAACCTC ATCTCCACCT GAAAATTGGC CCCAAACTGA TACTGAAAGC 
ACTTCTTCAG ACACCTCCAC CGGGATCCGC CTGGACTCCT GTCCTCCTCA ATGCTCCAAC 
CACCAGAATC TGCCACAGTT ATTGGCTGAA GGCACACTAG AGTTCCTCTC TGTTACTCCA 
GCGGAGGGGC TACACAAAGA GGGCATCGCC CGCCACAGTT CATCGCCCTC CATCGTCATG 
TTGCCAGACA GCACTGATGC AGCCACTCCA GACCGCAGCC TGCAGGTGGA GGACCCTCAG 
GCCCGCAAAC GTAGCCACAG CAGCACTCAG CTTAGCCTTA AAGGGAAGAT CATGGAGAAG 
CTGGTAGACA AGTCTCCAGG GGCAAAGCCC AAGATCAAGA AGACTAAGAG GAAAGAGGAA 
GAGAAGCAGA AAAAAACGAA CCAAGCTGAG AAACTGCATC CACTCAGTAT TTTCTTTGGA 
GACAGTCTAG ACCTCGAGAA CTGGTACAGC TGTGGTGAAG GGGAGGTGTC GGAGATCGAG 
AGTGACATGG GCTCACCGAG CGGGGGCTCT TGCGGGACCG GTGCTGGGGC GAACGTCACA 
GGGCGCAGAT CGTCCTCCAC TCTGCCTCGT TTTAACATCC ATCCTCTCTA CCAGCACGTC 
CTGCTCTACC TACAGCTCTA CGACTCCTCT AGGACCCTGC ATGCCCTGTC GGCGATCGCA 
GCCATGGTGA GAGCCGCTCC CTCAGGCTTT GTAAGCGCCA TCTCCACCAC CAGCATCAAT 
AACACCTACA CTCCACAGCT CTCCTTGCTG CAAAACCTAC TTGCTCGCCA CAGAGTCTCT 
GTTATGGGCA AAGACTTCTA CTGCCCCATT CCCCAGGACT CTCACTCCCA TTCTTTCCGC 
AGTGCCATGT ACCTGGAGAT CATCATCTCC CTCTGTCTCT ACTTCCTGAG AAGCTATTAC 
TCTGCCCATG TGACATTGGG TCCTCAGGAC TTGGCAGGAA ACCGGGCCAT GCAGCTGACC 
AGCGTGGAGG TCTTGACACT GCTATTCAGC GAACTAGCCA AAGTCACAGC TGGCTCGGCA 
AAGGGTTTTG CTAGCTTCAT CAGTGACGTC CTTTCCAAGT GCAAAGTTCA GAAAGTCATC 
CTCCATTGCC TGCTTTCCTC CATATTCAGC GCCCAGAAAT GGCATGACCA ACGGACGGCA 
GGGGTCAACG TGGCCACGGT GGAGGAAGGT CTCTCGGAGG ACAGCGTCAT CAACATGTCG 
GAGGACCAGA TAGACAGCTG CAGCGCCGTT CAGTCCCAGC TGCTCCGGCT GCTGCAGAGT 
CTAGTTGTAC TTGAGCATAG GGTCTTAATG CCAGCAGACG AAGGTGGAGA AGGAGGACCT 
GCCGCAGGAG GAGCAGGTGG AGGGCCAGGA AGCGGTGGAG TGGCAGGATT TGAGATCCTA 
GGAGGGGAGG TGGAGCATGT TAACCCTCAG CAGCCTATGA CGTCACTGCA GTACCTCCAT 
GGTCAGCCTA TTACAGCTCA GGGCATGTTC CTGTGTGCAG TCATCAGGGC CCTGCACCAG 
CACCATGCCT GTAAGATGCA CCCCCAATGG ATAGGGCTGA TCACAACCAC CCTGCCTTAC 
ATGGGGAGGG TCCTGAGGAG GGTGGTGGCC TCAGTGACTC TGCAGCTATG CAGAAACCTG 
GATAATCTCC TCCAGCAGTA CCGCTATGAA ACCAGCATAA CTGACACCAG ACCCCAGTGG 
ATGGCTCTCT GCATCCCTCC AGACCTGATT CTGACTGTGC TGGAGGGAAT CACTTCCATT 
ATCCATTACT GTCTGCTGGA CCCCACTTCA CAGTACCACC AGTTACAAGT GAGTGTTGAC 
CAGAAGCACC TGGCCGAGGC GCGTTCAGGT ATCCTGTCCA TCCTCCACAC CATCATGTCT 
TCCGTCACGC TGCTGTGGAG CGTGCTCCAC CAAGCCGACA GCTCTGACAA GCCAGCTGCC 
GCTTCAGCTG CCGCCACCTC AAACATCAAC CTAGGCTCCA CCAAGAATCT CCGTCAGCAG 
ATCTTGGAAC TGCTGGGTCC AATCTCCATG AACCACGGCG CTCATTTCAT GGCTGCAATT 
GCTTATGTTT GGAACGAGAG AAAACAAGTC AAGGCGCCAA TCAGAAATAA GGTCATCCCT 
GTAGCGAGTG AGGAGCAGCT GCTGCTGGTT GAGCTCGTTC GGTCGGTCAG CGCCATGCGC 
ACTGAGACTG TTATGCAGAC AGTGAAGGAG GTCCTGAAGC AGCCACCTGC TATCGCAAAA 
GACAAGAAGC ACATCTCTTT GGAAGTCTGC ATGCTACAGT TCTTCTATGC CTATGTACAG 
AGGATTCCTG TTTCCAGTTT GCTTGATAGC TGGCCGTCTC TGCTGGCGCT GCTGAAGGAC 
TCGGTCCAGT TGGGTCTGCC TGCTCCTGGG CAGTTTCTCA TACTGGGAGT TCTGAATGAG 
TTTATCTTGA AGAATCCCAA CCTGGAGAGT AAGAAAGACC AGCGAGAGCT GCAGGATGTG 
ACTCACAAGG TGGTGGAGGC CATCGGCACC ATCGCTGGCT CCTCTTTGGA ACAAACCACG 
TGGCTAAGGA GGAACCTGGA GGTGAAGGCA TCGCCTCAGA TTGTTGTGGA CGGAACCAAC 
CTGGAGGCAG ACGTAGAAGA CTTAATGCTC ACAGTGATGG AGGCCTCCAG CTTCACTCCA 
TCTGTGTACA GTGTTCACGC CCTCACGCTG CTGGCCGAGG TGCTGGCTCA TCTATTGGAC 
ATGGTGTTCT ACAGTGACGA GAAGGAGAAA GTCATCCCTC TGCTGGTTAA CATTATGCAC 
TACGTAGTTC CCTACCTCCG CAACCACAGT GCTCACAATG CCCCCAGCTA CCGAGCCTGC 
ATCCAGCTGC TGAGCAGCCT GAGCGGCTAC CAGTACACCC GCCGTGCCTG GAAAAAAGAG 
GCCTTCGACC TCTTCATGGA CCACACCTTC TTCCAGATGG ATGCTTCGTG TGTCAGCCAC 
TGGAGAGCCA TCATTGACCA CCTGATGACT CATGACAAGA CCACATTTAG AGACCTGATG 
ACCCGGGTGG CGGTGGCTCA GAGCAGCTCC CTGAGTTTGT TCACCAACAG AGACGCTGAG 
CTGGAGCAGA GGGCCATGCT GCTCAAACGC CTGGCCTTCA CCATCTACAG CAGCGAGGTG 
GACCAGTACC AGAAGTACCT GCCGGACATC CAAGAGCGTC TGGTGGAGAG CCTGCGTCTG 
CCTCAGGTAC CCATCCTCCA CGCTCAGGTG TTCCTGTTCT TCAGAGTGCT GCTGCTGCGC 
ATGTCGCCTC AACACCTCAC GTCACTCTGG CCCACCATGA TCACTGAGCT GGTCCAAGTG 
TTTCTGCTGA TGGAGCAGGA GTTAACCGCT GACGAGGACA TATCAAGGAC GTCAGGACCG 
TCGGTTGTCG GGTTGGAGAC CACCTACTCT GGAGGGAACG GCTTCTCCAC GTCCTACAAC 
AGCCAGCGCT GGCTCAACCT CTACCTCTCT GCTTGCAAGT TCCTGGATCT TGCCTTGGCG 
TTGCCTCCTG AGAGTCTCCC TCAGTTCCAG ATGTACCGCT GGGCCTTCAT CCCAGAGGCT 
TCAGACGATT CAGGGTTGGA AGTGAGACGC CAGGGGACGC ATCAGAGGGA GTTCAAGCCC 
TACGTGGTCC GTCTGGTGAA GCTGCTGAGA AAACGAGCCA AGAAAAACCC AGAGGAGGAC 
TGTTCCACTA AAACCCTGCC GTGGGAGCCG GGCCACCTGA TGCTGACCCT CTACGTCATC 
CGCAGCATGG AGCAGCTGCT GCCCTTCTTC AACCTGCTCA GCCAGGTCTT CAACAGCAGG 
GCGAGCTGCC GATCGGGGCC CGTCTACACC CACAATCCCG TGGATTCCTC CTTCCCCGCC 
AGCAAGGAGG GCCACAAGCT GGAGAGCCAG AAGGTGTTCT GGAGCCGAGC CCGACAGAAC 
ATCGAGGAGA TGGTGGAAAA AGACTTTCTA GAGGGTCTTA TCAAGACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023186395.1
CDS	395..7825
Translation	MNAEEVELLSDSKYRNYVAAVDKALKNFEYSSEWADLISALGKLNKVLQNNAKYQVVPKKLTIGKRLAQCLHPALPSGVHRKALETYEIIFKIIGPKRLAKDLFLYSSGLFPLLSNAAMSVKPVLLGLYETYYLPLGKTLKPGLQGLLTGVLPGLEEGSEYYDRTNTLLEKVAAAVEQSAFYSALWGSILTSPSVRLPGVSFVLLHLNRKLSMEDQLYVMGSDIELMVEAVSTSVQDSSVLVQRSTLDLILFCFPFHMSQATRPDMIRILSAALHVVLRRDMSLNRRLYAWLLGPRSTRQSNPEEHASHYFNSFSKDMLVQAMVGILQGKARGGEEESILMHDLKPFRILISLLDKPELGPAILEDVLIEVFRTLYTQCRTELDLQNQSPFSKDHTHLSSKLRENKKTAELIKTANLLFNSFEPYYMWDYIARWFEECCRRTLNRSTRVARYGWSLDSPDLSLVEFCQLVDFLLDIVSLPTRSMRVICQETYIEIQTEHLPQLLLRMVAALTCHLQALDLGELTHCLRLCSKILSKVQPPLVSPLALPVSPQPPGLAAQNKSRDVDVKQTPPSVPEMTDSGDVFDDGEIPTGSRSAESGFTDFVQYREDGSEDPERASHPHPPLRAGSRPSGSCQSKPLDKPVMQCCLEHFQQFLSCLITFYIVPEHLDKAEGQRGELLHSGPLVSAGSQPSNYTEPCSGAGVVQKDCLAAFTAACQLFLECSSFPVYIAEGNLKSSPAQEEQCDSEQVRLPVWLQTLMDACCLAGDFSLQGVAISLLMDLIGLTQSVAVVTAESLASSSSTEPTQPMSPSQGRVAVVIRPPLTQGILKYIADKTDFFKSVAVILWDQLGEGTPQHHQRSVELFYQLHNLVPSSSTCEDVISQQLMHRDKRIRLAAHVKFSVLWHLTRDLNITKSSPFSRTFDRSLFIMLDSLSYWDPCTSAVGRAWLNQVLQRHDIARVLEPLLLLLLHPKTHRVSIQKVQAQRHWAQIFPDPPEQEPTEPIYIRDSGFSDNFLQIRSEGGGHEDFRGLTIGDMEPFCLTVNPLSDSLSILSLSSENLHLAGEYQTRDQQEEQQSSKSSGSHSSTLENGSFEDPEGVSYIGNGSDRPLGSSDETPDDSIEEAVSCVVKDLIDRVLNLVDEESNETSSPPENWPQTDTESTSSDTSTGIRLDSCPPQCSNHQNLPQLLAEGTLEFLSVTPAEGLHKEGIARHSSSPSIVMLPDSTDAATPDRSLQVEDPQARKRSHSSTQLSLKGKIMEKLVDKSPGAKPKIKKTKRKEEEKQKKTNQAEKLHPLSIFFGDSLDLENWYSCGEGEVSEIESDMGSPSGGSCGTGAGANVTGRRSSSTLPRFNIHPLYQHVLLYLQLYDSSRTLHALSAIAAMVRAAPSGFVSAISTTSINNTYTPQLSLLQNLLARHRVSVMGKDFYCPIPQDSHSHSFRSAMYLEIIISLCLYFLRSYYSAHVTLGPQDLAGNRAMQLTSVEVLTLLFSELAKVTAGSAKGFASFISDVLSKCKVQKVILHCLLSSIFSAQKWHDQRTAGVNVATVEEGLSEDSVINMSEDQIDSCSAVQSQLLRLLQSLVVLEHRVLMPADEGGEGGPAAGGAGGGPGSGGVAGFEILGGEVEHVNPQQPMTSLQYLHGQPITAQGMFLCAVIRALHQHHACKMHPQWIGLITTTLPYMGRVLRRVVASVTLQLCRNLDNLLQQYRYETSITDTRPQWMALCIPPDLILTVLEGITSIIHYCLLDPTSQYHQLQVSVDQKHLAEARSGILSILHTIMSSVTLLWSVLHQADSSDKPAAASAAATSNINLGSTKNLRQQILELLGPISMNHGAHFMAAIAYVWNERKQVKAPIRNKVIPVASEEQLLLVELVRSVSAMRTETVMQTVKEVLKQPPAIAKDKKHISLEVCMLQFFYAYVQRIPVSSLLDSWPSLLALLKDSVQLGLPAPGQFLILGVLNEFILKNPNLESKKDQRELQDVTHKVVEAIGTIAGSSLEQTTWLRRNLEVKASPQIVVDGTNLEADVEDLMLTVMEASSFTPSVYSVHALTLLAEVLAHLLDMVFYSDEKEKVIPLLVNIMHYVVPYLRNHSAHNAPSYRACIQLLSSLSGYQYTRRAWKKEAFDLFMDHTFFQMDASCVSHWRAIIDHLMTHDKTTFRDLMTRVAVAQSSSLSLFTNRDAELEQRAMLLKRLAFTIYSSEVDQYQKYLPDIQERLVESLRLPQVPILHAQVFLFFRVLLLRMSPQHLTSLWPTMITELVQVFLLMEQELTADEDISRTSGPSVVGLETTYSGGNGFSTSYNSQRWLNLYLSACKFLDLALALPPESLPQFQMYRWAFIPEASDDSGLEVRRQGTHQREFKPYVVRLVKLLRKRAKKNPEEDCSTKTLPWEPGHLMLTLYVIRSMEQLLPFFNLLSQVFNSRASCRSGPVYTHNPVDSSFPASKEGHKLESQKVFWSRARQNIEEMVEKDFLEGLIKT

Target ORF information:

RefSeq Version	XM_023330627.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus dopey family member 1 (dopey1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023330627.1

ORF Insert Sequence:

ATGAATGCAG AGGAAGTGGA GCTGCTCAGC GACTCAAAGT ACAGGAACTA TGTGGCAGCA 
GTGGACAAAG CCCTCAAGAA CTTTGAGTAT TCCAGCGAGT GGGCCGACCT CATCTCCGCA 
CTGGGCAAGC TCAACAAGGT TTTGCAGAAC AATGCAAAAT ATCAAGTTGT CCCCAAGAAG 
CTGACAATAG GCAAACGGCT GGCCCAGTGC CTCCACCCAG CCCTGCCCAG CGGCGTGCAC 
CGGAAGGCCT TGGAGACCTA CGAGATCATT TTCAAGATCA TTGGGCCCAA GAGGCTGGCC 
AAAGACCTCT TTCTGTACAG CTCGGGACTC TTCCCACTAC TCTCCAACGC TGCTATGTCT 
GTGAAGCCCG TGTTGCTGGG GCTCTATGAG ACCTACTACC TGCCCCTGGG GAAGACTCTC 
AAACCAGGCC TGCAGGGCTT ACTGACCGGC GTGCTGCCTG GTCTGGAGGA AGGTTCAGAG 
TATTACGACA GAACCAACAC GCTGTTGGAG AAAGTTGCTG CAGCCGTGGA GCAGTCGGCC 
TTCTACAGTG CCTTGTGGGG CAGCATCCTG ACCAGCCCCT CTGTGCGTCT CCCCGGGGTC 
TCCTTCGTTC TGCTGCACCT CAACCGGAAG CTCTCCATGG AGGACCAGCT CTACGTCATG 
GGCAGCGACA TTGAGCTTAT GGTGGAGGCT GTTAGTACCT CGGTGCAGGA CTCAAGCGTT 
TTGGTCCAGA GAAGCACTCT GGACCTCATC CTCTTCTGCT TCCCCTTCCA TATGAGCCAG 
GCTACCCGCC CTGACATGAT CCGGATCCTT TCAGCAGCTT TGCATGTGGT TCTGAGAAGA 
GACATGTCTC TGAACCGCAG ACTCTACGCC TGGCTGCTGG GCCCCCGCAG TACTCGGCAG 
AGCAACCCGG AGGAACACGC TTCCCACTAC TTCAACAGCT TCTCCAAGGA CATGCTCGTC 
CAGGCAATGG TTGGGATTCT GCAGGGCAAG GCTCGAGGTG GGGAGGAGGA GAGCATCCTG 
ATGCACGACC TCAAGCCATT TCGCATCCTG ATCAGTCTGC TGGACAAACC AGAACTAGGG 
CCGGCCATTC TAGAGGACGT TCTGATCGAA GTGTTTCGGA CTCTTTACAC ACAGTGCCGG 
ACAGAGTTGG ACCTTCAAAA CCAGAGTCCG TTCAGCAAAG ATCACACACA CCTCAGCAGT 
AAACTGCGAG AAAACAAGAA GACGGCAGAG CTTATCAAAA CAGCCAATCT CCTGTTCAAC 
TCGTTTGAGC CGTACTACAT GTGGGACTAC ATCGCTCGCT GGTTTGAGGA GTGCTGCAGG 
AGAACATTGA ACCGCAGCAC ACGTGTTGCA CGATACGGTT GGAGTCTGGA TTCTCCTGAT 
CTTTCTCTGG TGGAGTTCTG TCAGTTGGTA GATTTTCTAC TAGACATTGT TTCATTGCCT 
ACTAGAAGCA TGAGGGTAAT CTGCCAGGAG ACGTACATAG AAATCCAGAC GGAGCATCTC 
CCTCAACTGC TGTTGCGGAT GGTGGCAGCT CTGACCTGTC ATCTCCAGGC GCTGGACCTC 
GGGGAGCTCA CCCACTGCCT TCGCCTCTGC TCCAAGATCC TCAGCAAGGT GCAGCCTCCG 
TTGGTGTCCC CGCTCGCCCT CCCCGTGAGT CCACAGCCGC CGGGCCTGGC GGCACAGAAC 
AAAAGCAGAG ATGTGGATGT TAAACAGACT CCTCCCTCTG TCCCAGAGAT GACCGACAGT 
GGGGACGTGT TTGACGATGG GGAAATCCCT ACTGGCAGTC GCTCTGCAGA AAGTGGCTTC 
ACAGACTTTG TTCAATACCG AGAAGATGGT TCCGAGGATC CAGAGCGAGC CTCTCATCCC 
CACCCGCCTC TTAGAGCAGG CAGTCGTCCG TCAGGCTCGT GTCAGTCCAA GCCTTTGGAC 
AAACCAGTCA TGCAGTGTTG CCTGGAGCAC TTCCAGCAGT TTCTCTCCTG TCTGATCACT 
TTTTATATTG TTCCTGAGCA CCTGGACAAA GCTGAGGGTC AGAGAGGTGA GCTCTTGCAT 
TCGGGGCCTT TGGTTTCCGC AGGTTCCCAG CCCAGTAATT ATACGGAGCC GTGCTCAGGA 
GCTGGAGTGG TCCAGAAAGA TTGTCTGGCT GCATTTACTG CTGCCTGTCA GCTCTTCTTA 
GAATGCTCCA GTTTCCCAGT CTACATTGCA GAGGGCAACC TCAAGTCCTC GCCTGCACAG 
GAAGAGCAAT GCGACAGTGA GCAGGTTCGG CTGCCAGTGT GGCTGCAGAC CCTGATGGAT 
GCCTGCTGCC TGGCTGGTGA CTTCAGCCTA CAGGGTGTTG CCATCTCCCT GCTTATGGAC 
CTAATAGGCC TGACTCAGTC AGTTGCTGTG GTGACGGCAG AGAGTCTGGC TTCCAGTAGC 
AGTACGGAGC CCACTCAGCC AATGAGCCCC AGCCAGGGTC GGGTGGCTGT GGTCATTAGG 
CCTCCACTCA CTCAGGGAAT CCTGAAGTAT ATTGCGGATA AAACGGACTT CTTCAAGAGC 
GTTGCAGTGA TATTATGGGA CCAGCTGGGT GAAGGAACTC CTCAGCATCA CCAGCGCAGT 
GTTGAGCTCT TTTATCAGCT ACACAACCTG GTCCCTTCAT CCAGCACCTG TGAGGACGTC 
ATAAGCCAGC AACTCATGCA CAGAGACAAG AGAATTCGTT TGGCAGCCCA CGTGAAGTTC 
TCTGTTCTGT GGCATTTAAC GCGAGACTTG AACATCACCA AGTCTTCTCC GTTTAGTCGC 
ACATTTGACA GGTCTCTCTT CATCATGCTC GACAGCTTAA GTTATTGGGA TCCATGTACC 
AGTGCGGTCG GTCGGGCTTG GTTAAATCAA GTCCTTCAGA GACATGACAT TGCACGGGTT 
CTTGAGCCAC TCCTACTTCT TCTGCTCCAT CCTAAAACTC ACAGGGTTTC TATTCAGAAG 
GTGCAAGCCC AACGCCATTG GGCGCAGATA TTTCCTGATC CACCTGAGCA GGAACCAACA 
GAACCAATCT ACATTAGAGA CTCCGGCTTC TCAGACAACT TTCTTCAGAT TCGAAGCGAA 
GGGGGTGGGC ATGAGGACTT CAGAGGTCTG ACAATAGGTG ACATGGAGCC ATTTTGCCTG 
ACGGTAAACC CCCTAAGTGA CAGCTTGTCC ATTCTTAGCC TGAGCAGTGA AAACTTGCAC 
TTAGCTGGTG AGTATCAGAC CAGGGACCAG CAGGAGGAGC AACAGAGTTC AAAATCAAGT 
GGTTCTCATT CATCTACACT GGAAAACGGT AGCTTTGAAG ACCCTGAGGG GGTCAGCTAC 
ATCGGAAATG GCTCAGATCG ACCGCTTGGT TCATCTGATG AAACGCCTGA CGACTCCATA 
GAGGAAGCTG TGTCTTGTGT AGTGAAGGAC CTGATTGATA GGGTTCTGAA TTTAGTTGAT 
GAGGAATCAA ATGAAACCTC ATCTCCACCT GAAAATTGGC CCCAAACTGA TACTGAAAGC 
ACTTCTTCAG ACACCTCCAC CGGGATCCGC CTGGACTCCT GTCCTCCTCA ATGCTCCAAC 
CACCAGAATC TGCCACAGTT ATTGGCTGAA GGCACACTAG AGTTCCTCTC TGTTACTCCA 
GCGGAGGGGC TACACAAAGA GGGCATCGCC CGCCACAGTT CATCGCCCTC CATCGTCATG 
TTGCCAGACA GCACTGATGC AGCCACTCCA GACCGCAGCC TGCAGGTGGA GGACCCTCAG 
GCCCGCAAAC GTAGCCACAG CAGCACTCAG CTTAGCCTTA AAGGGAAGAT CATGGAGAAG 
CTGGTAGACA AGTCTCCAGG GGCAAAGCCC AAGATCAAGA AGACTAAGAG GAAAGAGGAA 
GAGAAGCAGA AAAAAACGAA CCAAGCTGAG AAACTGCATC CACTCAGTAT TTTCTTTGGA 
GACAGTCTAG ACCTCGAGAA CTGGTACAGC TGTGGTGAAG GGGAGGTGTC GGAGATCGAG 
AGTGACATGG GCTCACCGAG CGGGGGCTCT TGCGGGACCG GTGCTGGGGC GAACGTCACA 
GGGCGCAGAT CGTCCTCCAC TCTGCCTCGT TTTAACATCC ATCCTCTCTA CCAGCACGTC 
CTGCTCTACC TACAGCTCTA CGACTCCTCT AGGACCCTGC ATGCCCTGTC GGCGATCGCA 
GCCATGGTGA GAGCCGCTCC CTCAGGCTTT GTAAGCGCCA TCTCCACCAC CAGCATCAAT 
AACACCTACA CTCCACAGCT CTCCTTGCTG CAAAACCTAC TTGCTCGCCA CAGAGTCTCT 
GTTATGGGCA AAGACTTCTA CTGCCCCATT CCCCAGGACT CTCACTCCCA TTCTTTCCGC 
AGTGCCATGT ACCTGGAGAT CATCATCTCC CTCTGTCTCT ACTTCCTGAG AAGCTATTAC 
TCTGCCCATG TGACATTGGG TCCTCAGGAC TTGGCAGGAA ACCGGGCCAT GCAGCTGACC 
AGCGTGGAGG TCTTGACACT GCTATTCAGC GAACTAGCCA AAGTCACAGC TGGCTCGGCA 
AAGGGTTTTG CTAGCTTCAT CAGTGACGTC CTTTCCAAGT GCAAAGTTCA GAAAGTCATC 
CTCCATTGCC TGCTTTCCTC CATATTCAGC GCCCAGAAAT GGCATGACCA ACGGACGGCA 
GGGGTCAACG TGGCCACGGT GGAGGAAGGT CTCTCGGAGG ACAGCGTCAT CAACATGTCG 
GAGGACCAGA TAGACAGCTG CAGCGCCGTT CAGTCCCAGC TGCTCCGGCT GCTGCAGAGT 
CTAGTTGTAC TTGAGCATAG GGTCTTAATG CCAGCAGACG AAGGTGGAGA AGGAGGACCT 
GCCGCAGGAG GAGCAGGTGG AGGGCCAGGA AGCGGTGGAG TGGCAGGATT TGAGATCCTA 
GGAGGGGAGG TGGAGCATGT TAACCCTCAG CAGCCTATGA CGTCACTGCA GTACCTCCAT 
GGTCAGCCTA TTACAGCTCA GGGCATGTTC CTGTGTGCAG TCATCAGGGC CCTGCACCAG 
CACCATGCCT GTAAGATGCA CCCCCAATGG ATAGGGCTGA TCACAACCAC CCTGCCTTAC 
ATGGGGAGGG TCCTGAGGAG GGTGGTGGCC TCAGTGACTC TGCAGCTATG CAGAAACCTG 
GATAATCTCC TCCAGCAGTA CCGCTATGAA ACCAGCATAA CTGACACCAG ACCCCAGTGG 
ATGGCTCTCT GCATCCCTCC AGACCTGATT CTGACTGTGC TGGAGGGAAT CACTTCCATT 
ATCCATTACT GTCTGCTGGA CCCCACTTCA CAGTACCACC AGTTACAAGT GAGTGTTGAC 
CAGAAGCACC TGGCCGAGGC GCGTTCAGGT ATCCTGTCCA TCCTCCACAC CATCATGTCT 
TCCGTCACGC TGCTGTGGAG CGTGCTCCAC CAAGCCGACA GCTCTGACAA GCCAGCTGCC 
GCTTCAGCTG CCGCCACCTC AAACATCAAC CTAGGCTCCA CCAAGAATCT CCGTCAGCAG 
ATCTTGGAAC TGCTGGGTCC AATCTCCATG AACCACGGCG CTCATTTCAT GGCTGCAATT 
GCTTATGTTT GGAACGAGAG AAAACAAGTC AAGGCGCCAA TCAGAAATAA GGTCATCCCT 
GTAGCGAGTG AGGAGCAGCT GCTGCTGGTT GAGCTCGTTC GGTCGGTCAG CGCCATGCGC 
ACTGAGACTG TTATGCAGAC AGTGAAGGAG GTCCTGAAGC AGCCACCTGC TATCGCAAAA 
GACAAGAAGC ACATCTCTTT GGAAGTCTGC ATGCTACAGT TCTTCTATGC CTATGTACAG 
AGGATTCCTG TTTCCAGTTT GCTTGATAGC TGGCCGTCTC TGCTGGCGCT GCTGAAGGAC 
TCGGTCCAGT TGGGTCTGCC TGCTCCTGGG CAGTTTCTCA TACTGGGAGT TCTGAATGAG 
TTTATCTTGA AGAATCCCAA CCTGGAGAGT AAGAAAGACC AGCGAGAGCT GCAGGATGTG 
ACTCACAAGG TGGTGGAGGC CATCGGCACC ATCGCTGGCT CCTCTTTGGA ACAAACCACG 
TGGCTAAGGA GGAACCTGGA GGTGAAGGCA TCGCCTCAGA TTGTTGTGGA CGGAACCAAC 
CTGGAGGCAG ACGTAGAAGA CTTAATGCTC ACAGTGATGG AGGCCTCCAG CTTCACTCCA 
TCTGTGTACA GTGTTCACGC CCTCACGCTG CTGGCCGAGG TGCTGGCTCA TCTATTGGAC 
ATGGTGTTCT ACAGTGACGA GAAGGAGAAA GTCATCCCTC TGCTGGTTAA CATTATGCAC 
TACGTAGTTC CCTACCTCCG CAACCACAGT GCTCACAATG CCCCCAGCTA CCGAGCCTGC 
ATCCAGCTGC TGAGCAGCCT GAGCGGCTAC CAGTACACCC GCCGTGCCTG GAAAAAAGAG 
GCCTTCGACC TCTTCATGGA CCACACCTTC TTCCAGATGG ATGCTTCGTG TGTCAGCCAC 
TGGAGAGCCA TCATTGACCA CCTGATGACT CATGACAAGA CCACATTTAG AGACCTGATG 
ACCCGGGTGG CGGTGGCTCA GAGCAGCTCC CTGAGTTTGT TCACCAACAG AGACGCTGAG 
CTGGAGCAGA GGGCCATGCT GCTCAAACGC CTGGCCTTCA CCATCTACAG CAGCGAGGTG 
GACCAGTACC AGAAGTACCT GCCGGACATC CAAGAGCGTC TGGTGGAGAG CCTGCGTCTG 
CCTCAGGTAC CCATCCTCCA CGCTCAGGTG TTCCTGTTCT TCAGAGTGCT GCTGCTGCGC 
ATGTCGCCTC AACACCTCAC GTCACTCTGG CCCACCATGA TCACTGAGCT GGTCCAAGTG 
TTTCTGCTGA TGGAGCAGGA GTTAACCGCT GACGAGGACA TATCAAGGAC GTCAGGACCG 
TCGGTTGTCG GGTTGGAGAC CACCTACTCT GGAGGGAACG GCTTCTCCAC GTCCTACAAC 
AGCCAGCGCT GGCTCAACCT CTACCTCTCT GCTTGCAAGT TCCTGGATCT TGCCTTGGCG 
TTGCCTCCTG AGAGTCTCCC TCAGTTCCAG ATGTACCGCT GGGCCTTCAT CCCAGAGGCT 
TCAGACGATT CAGGGTTGGA AGTGAGACGC CAGGGGACGC ATCAGAGGGA GTTCAAGCCC 
TACGTGGTCC GTCTGGTGAA GCTGCTGAGA AAACGAGCCA AGAAAAACCC AGAGGAGGAC 
TGTTCCACTA AAACCCTGCC GTGGGAGCCG GGCCACCTGA TGCTGACCCT CTACGTCATC 
CGCAGCATGG AGCAGCTGCT GCCCTTCTTC AACCTGCTCA GCCAGGTCTT CAACAGCAGG 
GCGAGCTGCC GATCGGGGCC CGTCTACACC CACAATCCCG TGGATTCCTC CTTCCCCGCC 
AGCAAGGAGG GCCACAAGCT GGAGAGCCAG AAGGTGTTCT GGAGCCGAGC CCGACAGAAC 
ATCGAGGAGA TGGTGGAAAA AGACTTTCTA GAGGGTCTTA TCAAGACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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DOPEY1 cDNA ORF clone, Xiphophorus maculatus(Southern platyfish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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