kif21b cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	kif21b
Entrez Gene ID	102218349
Full Name	kinesin family member 21B
Gene Type	protein-coding
Organism	Xiphophorus maculatus(southern platyfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005798016.1	XP_005798073.1	kinesin-like protein KIF21B
XM_023324952.1	XP_023180720.1	kinesin-like protein KIF21B isoform X4
XM_023324950.1	XP_023180718.1	kinesin-like protein KIF21B isoform X2
XM_023324949.1	XP_023180717.1	kinesin-like protein KIF21B isoform X1
XM_023324951.1	XP_023180719.1	kinesin-like protein KIF21B isoform X3
XM_023324955.1	XP_023180723.1	kinesin-like protein KIF21B isoform X6
XM_023324953.1	XP_023180721.1	kinesin-like protein KIF21B isoform X5

Pathways from BioSystems
Gene Ontology

The following kif21b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kif21b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXi03397	XM_005798016.1 Latest version!	Xiphophorus maculatus kinesin family member 21B (kif21b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXi31288	XM_023324949.1 Latest version!	Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXi31289	XM_023324950.1 Latest version!	Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXi31290	XM_023324951.1 Latest version!	Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXi31291	XM_023324952.1 Latest version!	Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXi31292	XM_023324953.1 Latest version!	Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXi31293	XM_023324955.1 Latest version!	Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kif21b ( XM_005798016.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_005798073.1 Xiphophorus maculatus kinesin family member 21B (kif21b), mRNA.

Price & Availability↑

CloneID	OXi03397
Clone ID Related Accession (Same CDS sequence)	XM_005798016.1
Accession Version	XM_005798016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1445702400000
Organism	Xiphophorus maculatus(Southern platyfish)
Product	kinesin-like protein KIF21B
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005372200.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xiphophorus maculatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGCGAGCC AGAGCGATTG CTGTGTGAAG GTTGCGTTGA GGATTCGTCC TCAGATGGCC 
AAGGAGAAGA TCGAGGGCTG TCACGTGTGC ACGCTGGTCA CACCTGGAGA ACCGCAGGTC 
CTACTGGGAA AGGACAAGGC CTTCACATAC GACTTTGTGT TCGATATTGA CTCAGAGCAG 
CAGCACATCT ACCAGACCTG TGTGTACAAG CTCATCGAGG GCTGCTTCGA AGGCTACAAC 
GCAACCGTGT TTGCTTATGG ACAGACGGGC TCAGGGAAGA CCTACACCAT GGGGACGGGT 
TTTGATGTGA ATCCGAGCCT GCAGGAGCAG GGCATCATCC CACGGGCCGT TCACCACCTG 
TTTGAGGGCA TCCAGAGCAG GAGGGATCGA GCCCAGGAGA TCGGCATCCA GGCACCAGAG 
TTTAAAGTCA GCGCCCAGTT TTTGGAGTTG TACAATGAGG AGATCCTGGA CTTGTTTGAC 
GGAAGTAGAG ATCCAGAGAA TCGCAGCAGG AAGTCCAACA TCAGAATCCA CGAGGACGCC 
AACGGCAGCA TCTACACCAC AGGAGTCACT TCCAGACTGG TGCAGTCAGA GGACGAGCTG 
CTGCAGTGCC TGAAGCTTGG GGCTCTGTCC CGTACCACAG CCAGCACACA GATGAACGCA 
CAAAGCTCCC GCTCACATGC CATCTTCACC ATCCATCTCT GTCAGATGAG GGTCTTTCAG 
CAGCAGCAAA TGGTAAGACT GGAGAATGGC GAAGTGAACG GCGTAGACTC CTCCCCCATC 
GCCCAGCCGG AGTACGAGAC CCTGATGGCC AAGTTTCACT TCGTGGACCT GGCCGGCTCA 
GAAAGGCTCA AACGCACGGG GGCCACTGGA GAAAGGGCCC GAGAGGGAAT CTCTATTAAC 
TGTGGACTGC TGGCTCTTGG AAATGTAATC AGCGCTTTAG GTGATCAGAC TAAGAAAGCC 
GGACACGTCC CATATAGAGA CTCCAAACTT ACCCGACTGT TGCAAGACTC GCTGGGAGGC 
AACAGCCACA CAGTCATGAT TGCCTGCGTC AGCCCGTCGG ACCGGGACTT CATGGAGACG 
CTGAACACAC TGAAGTACGC CAACCGTGCC CGAAACATCA AGAACAAGGT GGTGGTGAAC 
CAAGACAAGA CCAGCCAGCA GATCAACGCC CTACGTGCGG AGGTGGCCCG ACTCCAGATG 
GAGCTGATGG AGTACAAGGC GGGTAAGCGT GTGGCAGGTG AGGATGGGGC TGAAGGCTAC 
AGCGATCTGT ACCAGGAGAA CGCCATGCTG CAGAGAGAAA ACGACTCGCT GCGCCTGAGA 
GTAAAGGCCA TGCAGGAAAC CATCGATCAT CTCAACACAC GGGTGACACA TCTGCTGGCC 
AATGAGGTCA GCACTCTGCT CACCAAGTCA AGTGAAGGCA ATGAGGAGAT TGGAACGCTA 
ATACAGAACT ACATCCGAGA AGTTGAAGAA CTCAGATCCA AACTCCTTGA GAGCGAGGCC 
ATGAATGAGT CACTACGTCG ACAGGTGGCA CGGCTCTCCT CTCGTTCCTC GTTTCCAGCC 
TCCGCTCTAA GCCCAGCCCC TGGCCATCCC CCTGGCTCTT CCCCTATATC CATGTCCATG 
GAGGCCGAGA TGACAGATGT TCTGCGGCGC GCCAAGTTGG ACATCGAGCG GCTGAAGAAG 
AAGGAAAGAC GGCAGAGAAG GCTGAGCCCA GAGTTGGAGA AAGGTCTCAA GAAACGAGTA 
AAGCTGCACA CTCAGGAAAA CGGGGAGAAT CGGCGAAGCG GCGAAAATGG ACAGAATGGA 
GAGATCGATT CAGATGACAA TTACACCGAG GATATCATGT CTCCGCTGCA GGAGGAGAGT 
GGCTGTGAGG AAGATGAAGG CGAAGATGAG GAGGAGGGCA GGGAGGAAGA GGATGATTTT 
GACAGTGACG AGAGCCTAGT GGATTCAGAT TCTGACTCGG ATGAGAAAGC CAACTTCCAG 
GCAGATCTTG CCGACCTAAC CTGTGAGATT GAGATCAAGC AGAAGCTGAT CGATGAGCTG 
GAGAACAGCC AGCGGCGGCT GCTGATGTTA AAGCTGCAGT ACGAGGAGAA GCTCATCCTG 
CTGCAGAACA AGATCAGAGA CACGCAGCTG GAGAGAGACC GCGTGCTGCA GAACCTCATG 
TCCATGGAAA ACTACACTGA GGAGAAGGCC AGCAAGATCA GGCAGGAGTA CGAGAAGCGC 
CTCAAAGAAA TGAACCGTGA TCTGCTGAAG CTGCAGGCAG CGCAGAAGGA GCATGCACGT 
CTGCTCAAAA ACCAGGGGAG GTACGAGCGG GAACTGAAGA AGCTCCAAAC TGAAGTCAAC 
GAGATGAAGA AAGCAAAGGT GGTGCTGATG AAGCAAATGA AAGAGGAGCA ACAGAGAAGG 
AGGATGGTGG AGGCAAAGAG GAATCGAGAG ATTGCTCAGC TTAAGAAGGA GCAGCGGCGA 
CAGGAGTACC AGATTCGGGC GCTGGAGTCT CAAAAGAGGC AGCAGGAGCT GGTGCTGCGC 
AGGAAGACCC AGGAAGTGAC GGCCCTGCGG CGCATGGCCA AGCCCATGTC GGACCGCGTG 
GCGGGTCGAG TTGCCCGCTG GAACCAGACG CCTCCAGTTA CGGACTCCGG AGCTGATTTG 
TCGGCCAGCA CAGCGGCAAG CAGCTCCGAG GCAGAGACTG CGAGGACCGT GAGCGGGCTG 
GTGCGCCAGT GGAACAACAA GAACAACGCG TATGGATACA TGGCGGAGAG CGAGGGGAGT 
ATGGATGGAA CACGGGTGAT TAGGAAGAAG CTGCAGAGGA AGCCAGTGGG CCTGGGCAGC 
ACCGCACCTG CAGGCAGAGC CAACAACTTC TCCAAGTCTG CCCGTCTAAA GTGGCAAACC 
CTTGAGCGTC GCATCATGGA CATCGTCATG CAGAGAATGA CCATCTCCAA CGTGGAAGCA 
GACATGGATC GCCTTATCAA GAAACGAGAA GAGCTGACAA TGCAGCAGGA AGCTCTCTCA 
CATAAAAGGG AGATACTGAT GGCCGACGGA GAGGGGCCAG AGGCGGAGGA CCGCCTCCTT 
CAGGAAGTTA ACGAGGAGAT CGAGGTGCTG AATGCCAACA TAGACTACAT CAATGACAGT 
CTGTCGGACT GCCAGGCCAC CATCGTGCAG ATAGAGGAGA CCAAGGATGA ACTGGACTCG 
GTGGATACCT CAGTGGTGAT CAGCTCCTGT TCCCTGGCTG AAGCACGCCA CCTGCTGGAT 
CACTTCCTGA AGGCTTCCAT TGATAAGAGC TTACAGGTGG CTCAGAGGGA GGCGCAGGTA 
CGACTGCTCG AGGGTCAGCT GAGGCAGACT GATGTGATCG GCTCGTCTCA CAATCACATG 
ATCCTCGACG CGCTGCGAGA AAAAGCAGAG TGCATCCCTG AGCTCCAGGC TCTCATCCAC 
AACGTCCAGC AAGAAAATGG TTACGCCAGC ACGGACGAGG AGCCCTCTGA GTTCAGCCAA 
GCCTCTGACA GCAGTGTTTC ACAGATGAAG GAATCCAACA GCCAGGATGA CTTTAAAGTA 
AAGATGGAGC CTCGCCTCTC GGCCCAGATG AAGGCGGTGT CTGCGGAGTA CCTCGGTCCC 
ATCCTGGATC CCTCATCAAG CAGCAAGCAG CAGCACATCA CTAAGTCCCT AGCCTCACTG 
ACTGACATCC AGGAGGATGG CTTAAGTCTC ATTCCAGGAA GTCTGGCACT GAGCGTTCCC 
CTCCGGGACC CTTTTCACAG GGAACGAGCG TCCCGCACAA TCAGCCTTCC TGTCAGAGGA 
CACACTTTTC CCAGGCAGTC TCGAGGTTAT GACACTTCCC CCATTACAAG AAGGAAATCT 
TACGACCGTT CGTACAGGCC CACTGATGTC TACACTCCCC CCTCCTCACC TCCTTTAAGG 
ACAAGGAATG ATCGCAACGT CTTCTCCAGA CTGACCAGCA ACCAAGCGCA AGGTTCTGCT 
CTGGACAAGT CGGATGACAG CGACTCCTCG CTCTCCGAGG TGCTCAGGGG TGTGATCAAT 
CCCATTGGAG GTGTAAAGGG GGGTCGGACA GCACCCCTCC AGTGTGTCTC TGTTGCCGAG 
GGCCACTCGA AGCCGGTTCT GTGTGTGGAC GCTACAGATG AGCTGCTTTT CACTGGATCT 
AAAGATCGCA CCTGTAAGAT GTGGAATCTA GTCACAGGTC AGGAGATTGC CACCCTCAAA 
GGGCACCCAA ACAACGTGGT GTCGGTAAAA TATTGTCCCT CTTCTGGTCT GGTCTTCAGC 
GTCTCCACCT CATACATCAA GGTGTGGGAC ATCCGTGACT CTGCAAAGTG CATACGCACA 
CTCACGTCAT CAGGGCAGGT AGTTACAGGA GACGCGTGCG CTGGCACAAC CACCCGGACA 
ATCACCTTTG CTCAGGGCGA GTGTCAGATC AACCAGATAG CGCTCAACCC ATTAGGATCT 
GTGCTCTATG CTGCTGCTGG AAATATTGTT CGGATGTGGG ACCTTAACAG GATGCAGGCA 
ACAGGCAAAC TCTCAGGTCA CATCGGTTCT GTCATGTGTC TGACAGTGGG ACAGTCTCTG 
CTGGGCAAAG ACCAGGTCAT CACTGGCTCC AAAGACCATT ACGTCAAGAT ATTTGATGTG 
GCAGAAGGAA CTCTGGGTAA TGTGGGTCCC GCTCATAACT TTGAGCCTCC GCACTATGAC 
GGCATTGAAT GTTTGGCCGT GCAGGGAGAC GTGCTGTTCA GCGGCTCGCG AGACAACGGT 
ATCAAGAAGT GGGAGCTGGA GCAGCAGGAG CTCACACAGC AAATCCCGAA TGCCCACAAG 
GACTGGGTGT GCGCTCTGGC CTATGTACCC GGCCGGCCCA TGTTGCTGAG CGCCTGTCGG 
GGTGGCATGC TCAAGGTGTG GAATGTGGAA AACTTTACGC CCATAGGAGA GGTCAGAGGT 
CATGACAGCC CCATTAATGC CATATGCACC AACTCCAGGC ATATCTTTAC AGCCTCCAGT 
GACTGCAGGG TGAAGTTGTG GAACTATGTG CCAGGCTTGA CCCCATGTCT TCCTCGTCGG 
GTCCTGGCCA TCAAGGGCCG AGCCACCAGT CTCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005798073.1
CDS	26..5104
Translation	MASQSDCCVKVALRIRPQMAKEKIEGCHVCTLVTPGEPQVLLGKDKAFTYDFVFDIDSEQQHIYQTCVYKLIEGCFEGYNATVFAYGQTGSGKTYTMGTGFDVNPSLQEQGIIPRAVHHLFEGIQSRRDRAQEIGIQAPEFKVSAQFLELYNEEILDLFDGSRDPENRSRKSNIRIHEDANGSIYTTGVTSRLVQSEDELLQCLKLGALSRTTASTQMNAQSSRSHAIFTIHLCQMRVFQQQQMVRLENGEVNGVDSSPIAQPEYETLMAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQTKKAGHVPYRDSKLTRLLQDSLGGNSHTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQINALRAEVARLQMELMEYKAGKRVAGEDGAEGYSDLYQENAMLQRENDSLRLRVKAMQETIDHLNTRVTHLLANEVSTLLTKSSEGNEEIGTLIQNYIREVEELRSKLLESEAMNESLRRQVARLSSRSSFPASALSPAPGHPPGSSPISMSMEAEMTDVLRRAKLDIERLKKKERRQRRLSPELEKGLKKRVKLHTQENGENRRSGENGQNGEIDSDDNYTEDIMSPLQEESGCEEDEGEDEEEGREEEDDFDSDESLVDSDSDSDEKANFQADLADLTCEIEIKQKLIDELENSQRRLLMLKLQYEEKLILLQNKIRDTQLERDRVLQNLMSMENYTEEKASKIRQEYEKRLKEMNRDLLKLQAAQKEHARLLKNQGRYERELKKLQTEVNEMKKAKVVLMKQMKEEQQRRRMVEAKRNREIAQLKKEQRRQEYQIRALESQKRQQELVLRRKTQEVTALRRMAKPMSDRVAGRVARWNQTPPVTDSGADLSASTAASSSEAETARTVSGLVRQWNNKNNAYGYMAESEGSMDGTRVIRKKLQRKPVGLGSTAPAGRANNFSKSARLKWQTLERRIMDIVMQRMTISNVEADMDRLIKKREELTMQQEALSHKREILMADGEGPEAEDRLLQEVNEEIEVLNANIDYINDSLSDCQATIVQIEETKDELDSVDTSVVISSCSLAEARHLLDHFLKASIDKSLQVAQREAQVRLLEGQLRQTDVIGSSHNHMILDALREKAECIPELQALIHNVQQENGYASTDEEPSEFSQASDSSVSQMKESNSQDDFKVKMEPRLSAQMKAVSAEYLGPILDPSSSSKQQHITKSLASLTDIQEDGLSLIPGSLALSVPLRDPFHRERASRTISLPVRGHTFPRQSRGYDTSPITRRKSYDRSYRPTDVYTPPSSPPLRTRNDRNVFSRLTSNQAQGSALDKSDDSDSSLSEVLRGVINPIGGVKGGRTAPLQCVSVAEGHSKPVLCVDATDELLFTGSKDRTCKMWNLVTGQEIATLKGHPNNVVSVKYCPSSGLVFSVSTSYIKVWDIRDSAKCIRTLTSSGQVVTGDACAGTTTRTITFAQGECQINQIALNPLGSVLYAAAGNIVRMWDLNRMQATGKLSGHIGSVMCLTVGQSLLGKDQVITGSKDHYVKIFDVAEGTLGNVGPAHNFEPPHYDGIECLAVQGDVLFSGSRDNGIKKWELEQQELTQQIPNAHKDWVCALAYVPGRPMLLSACRGGMLKVWNVENFTPIGEVRGHDSPINAICTNSRHIFTASSDCRVKLWNYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_005798016.1
Organism	Xiphophorus maculatus(Southern platyfish)
Definition	Xiphophorus maculatus kinesin family member 21B (kif21b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005798016.1

ORF Insert Sequence:

ATGGCGAGCC AGAGCGATTG CTGTGTGAAG GTTGCGTTGA GGATTCGTCC TCAGATGGCC 
AAGGAGAAGA TCGAGGGCTG TCACGTGTGC ACGCTGGTCA CACCTGGAGA ACCGCAGGTC 
CTACTGGGAA AGGACAAGGC CTTCACATAC GACTTTGTGT TCGATATTGA CTCAGAGCAG 
CAGCACATCT ACCAGACCTG TGTGTACAAG CTCATCGAGG GCTGCTTCGA AGGCTACAAC 
GCAACCGTGT TTGCTTATGG ACAGACGGGC TCAGGGAAGA CCTACACCAT GGGGACGGGT 
TTTGATGTGA ATCCGAGCCT GCAGGAGCAG GGCATCATCC CACGGGCCGT TCACCACCTG 
TTTGAGGGCA TCCAGAGCAG GAGGGATCGA GCCCAGGAGA TCGGCATCCA GGCACCAGAG 
TTTAAAGTCA GCGCCCAGTT TTTGGAGTTG TACAATGAGG AGATCCTGGA CTTGTTTGAC 
GGAAGTAGAG ATCCAGAGAA TCGCAGCAGG AAGTCCAACA TCAGAATCCA CGAGGACGCC 
AACGGCAGCA TCTACACCAC AGGAGTCACT TCCAGACTGG TGCAGTCAGA GGACGAGCTG 
CTGCAGTGCC TGAAGCTTGG GGCTCTGTCC CGTACCACAG CCAGCACACA GATGAACGCA 
CAAAGCTCCC GCTCACATGC CATCTTCACC ATCCATCTCT GTCAGATGAG GGTCTTTCAG 
CAGCAGCAAA TGGTAAGACT GGAGAATGGC GAAGTGAACG GCGTAGACTC CTCCCCCATC 
GCCCAGCCGG AGTACGAGAC CCTGATGGCC AAGTTTCACT TCGTGGACCT GGCCGGCTCA 
GAAAGGCTCA AACGCACGGG GGCCACTGGA GAAAGGGCCC GAGAGGGAAT CTCTATTAAC 
TGTGGACTGC TGGCTCTTGG AAATGTAATC AGCGCTTTAG GTGATCAGAC TAAGAAAGCC 
GGACACGTCC CATATAGAGA CTCCAAACTT ACCCGACTGT TGCAAGACTC GCTGGGAGGC 
AACAGCCACA CAGTCATGAT TGCCTGCGTC AGCCCGTCGG ACCGGGACTT CATGGAGACG 
CTGAACACAC TGAAGTACGC CAACCGTGCC CGAAACATCA AGAACAAGGT GGTGGTGAAC 
CAAGACAAGA CCAGCCAGCA GATCAACGCC CTACGTGCGG AGGTGGCCCG ACTCCAGATG 
GAGCTGATGG AGTACAAGGC GGGTAAGCGT GTGGCAGGTG AGGATGGGGC TGAAGGCTAC 
AGCGATCTGT ACCAGGAGAA CGCCATGCTG CAGAGAGAAA ACGACTCGCT GCGCCTGAGA 
GTAAAGGCCA TGCAGGAAAC CATCGATCAT CTCAACACAC GGGTGACACA TCTGCTGGCC 
AATGAGGTCA GCACTCTGCT CACCAAGTCA AGTGAAGGCA ATGAGGAGAT TGGAACGCTA 
ATACAGAACT ACATCCGAGA AGTTGAAGAA CTCAGATCCA AACTCCTTGA GAGCGAGGCC 
ATGAATGAGT CACTACGTCG ACAGGTGGCA CGGCTCTCCT CTCGTTCCTC GTTTCCAGCC 
TCCGCTCTAA GCCCAGCCCC TGGCCATCCC CCTGGCTCTT CCCCTATATC CATGTCCATG 
GAGGCCGAGA TGACAGATGT TCTGCGGCGC GCCAAGTTGG ACATCGAGCG GCTGAAGAAG 
AAGGAAAGAC GGCAGAGAAG GCTGAGCCCA GAGTTGGAGA AAGGTCTCAA GAAACGAGTA 
AAGCTGCACA CTCAGGAAAA CGGGGAGAAT CGGCGAAGCG GCGAAAATGG ACAGAATGGA 
GAGATCGATT CAGATGACAA TTACACCGAG GATATCATGT CTCCGCTGCA GGAGGAGAGT 
GGCTGTGAGG AAGATGAAGG CGAAGATGAG GAGGAGGGCA GGGAGGAAGA GGATGATTTT 
GACAGTGACG AGAGCCTAGT GGATTCAGAT TCTGACTCGG ATGAGAAAGC CAACTTCCAG 
GCAGATCTTG CCGACCTAAC CTGTGAGATT GAGATCAAGC AGAAGCTGAT CGATGAGCTG 
GAGAACAGCC AGCGGCGGCT GCTGATGTTA AAGCTGCAGT ACGAGGAGAA GCTCATCCTG 
CTGCAGAACA AGATCAGAGA CACGCAGCTG GAGAGAGACC GCGTGCTGCA GAACCTCATG 
TCCATGGAAA ACTACACTGA GGAGAAGGCC AGCAAGATCA GGCAGGAGTA CGAGAAGCGC 
CTCAAAGAAA TGAACCGTGA TCTGCTGAAG CTGCAGGCAG CGCAGAAGGA GCATGCACGT 
CTGCTCAAAA ACCAGGGGAG GTACGAGCGG GAACTGAAGA AGCTCCAAAC TGAAGTCAAC 
GAGATGAAGA AAGCAAAGGT GGTGCTGATG AAGCAAATGA AAGAGGAGCA ACAGAGAAGG 
AGGATGGTGG AGGCAAAGAG GAATCGAGAG ATTGCTCAGC TTAAGAAGGA GCAGCGGCGA 
CAGGAGTACC AGATTCGGGC GCTGGAGTCT CAAAAGAGGC AGCAGGAGCT GGTGCTGCGC 
AGGAAGACCC AGGAAGTGAC GGCCCTGCGG CGCATGGCCA AGCCCATGTC GGACCGCGTG 
GCGGGTCGAG TTGCCCGCTG GAACCAGACG CCTCCAGTTA CGGACTCCGG AGCTGATTTG 
TCGGCCAGCA CAGCGGCAAG CAGCTCCGAG GCAGAGACTG CGAGGACCGT GAGCGGGCTG 
GTGCGCCAGT GGAACAACAA GAACAACGCG TATGGATACA TGGCGGAGAG CGAGGGGAGT 
ATGGATGGAA CACGGGTGAT TAGGAAGAAG CTGCAGAGGA AGCCAGTGGG CCTGGGCAGC 
ACCGCACCTG CAGGCAGAGC CAACAACTTC TCCAAGTCTG CCCGTCTAAA GTGGCAAACC 
CTTGAGCGTC GCATCATGGA CATCGTCATG CAGAGAATGA CCATCTCCAA CGTGGAAGCA 
GACATGGATC GCCTTATCAA GAAACGAGAA GAGCTGACAA TGCAGCAGGA AGCTCTCTCA 
CATAAAAGGG AGATACTGAT GGCCGACGGA GAGGGGCCAG AGGCGGAGGA CCGCCTCCTT 
CAGGAAGTTA ACGAGGAGAT CGAGGTGCTG AATGCCAACA TAGACTACAT CAATGACAGT 
CTGTCGGACT GCCAGGCCAC CATCGTGCAG ATAGAGGAGA CCAAGGATGA ACTGGACTCG 
GTGGATACCT CAGTGGTGAT CAGCTCCTGT TCCCTGGCTG AAGCACGCCA CCTGCTGGAT 
CACTTCCTGA AGGCTTCCAT TGATAAGAGC TTACAGGTGG CTCAGAGGGA GGCGCAGGTA 
CGACTGCTCG AGGGTCAGCT GAGGCAGACT GATGTGATCG GCTCGTCTCA CAATCACATG 
ATCCTCGACG CGCTGCGAGA AAAAGCAGAG TGCATCCCTG AGCTCCAGGC TCTCATCCAC 
AACGTCCAGC AAGAAAATGG TTACGCCAGC ACGGACGAGG AGCCCTCTGA GTTCAGCCAA 
GCCTCTGACA GCAGTGTTTC ACAGATGAAG GAATCCAACA GCCAGGATGA CTTTAAAGTA 
AAGATGGAGC CTCGCCTCTC GGCCCAGATG AAGGCGGTGT CTGCGGAGTA CCTCGGTCCC 
ATCCTGGATC CCTCATCAAG CAGCAAGCAG CAGCACATCA CTAAGTCCCT AGCCTCACTG 
ACTGACATCC AGGAGGATGG CTTAAGTCTC ATTCCAGGAA GTCTGGCACT GAGCGTTCCC 
CTCCGGGACC CTTTTCACAG GGAACGAGCG TCCCGCACAA TCAGCCTTCC TGTCAGAGGA 
CACACTTTTC CCAGGCAGTC TCGAGGTTAT GACACTTCCC CCATTACAAG AAGGAAATCT 
TACGACCGTT CGTACAGGCC CACTGATGTC TACACTCCCC CCTCCTCACC TCCTTTAAGG 
ACAAGGAATG ATCGCAACGT CTTCTCCAGA CTGACCAGCA ACCAAGCGCA AGGTTCTGCT 
CTGGACAAGT CGGATGACAG CGACTCCTCG CTCTCCGAGG TGCTCAGGGG TGTGATCAAT 
CCCATTGGAG GTGTAAAGGG GGGTCGGACA GCACCCCTCC AGTGTGTCTC TGTTGCCGAG 
GGCCACTCGA AGCCGGTTCT GTGTGTGGAC GCTACAGATG AGCTGCTTTT CACTGGATCT 
AAAGATCGCA CCTGTAAGAT GTGGAATCTA GTCACAGGTC AGGAGATTGC CACCCTCAAA 
GGGCACCCAA ACAACGTGGT GTCGGTAAAA TATTGTCCCT CTTCTGGTCT GGTCTTCAGC 
GTCTCCACCT CATACATCAA GGTGTGGGAC ATCCGTGACT CTGCAAAGTG CATACGCACA 
CTCACGTCAT CAGGGCAGGT AGTTACAGGA GACGCGTGCG CTGGCACAAC CACCCGGACA 
ATCACCTTTG CTCAGGGCGA GTGTCAGATC AACCAGATAG CGCTCAACCC ATTAGGATCT 
GTGCTCTATG CTGCTGCTGG AAATATTGTT CGGATGTGGG ACCTTAACAG GATGCAGGCA 
ACAGGCAAAC TCTCAGGTCA CATCGGTTCT GTCATGTGTC TGACAGTGGG ACAGTCTCTG 
CTGGGCAAAG ACCAGGTCAT CACTGGCTCC AAAGACCATT ACGTCAAGAT ATTTGATGTG 
GCAGAAGGAA CTCTGGGTAA TGTGGGTCCC GCTCATAACT TTGAGCCTCC GCACTATGAC 
GGCATTGAAT GTTTGGCCGT GCAGGGAGAC GTGCTGTTCA GCGGCTCGCG AGACAACGGT 
ATCAAGAAGT GGGAGCTGGA GCAGCAGGAG CTCACACAGC AAATCCCGAA TGCCCACAAG 
GACTGGGTGT GCGCTCTGGC CTATGTACCC GGCCGGCCCA TGTTGCTGAG CGCCTGTCGG 
GGTGGCATGC TCAAGGTGTG GAATGTGGAA AACTTTACGC CCATAGGAGA GGTCAGAGGT 
CATGACAGCC CCATTAATGC CATATGCACC AACTCCAGGC ATATCTTTAC AGCCTCCAGT 
GACTGCAGGG TGAAGTTGTG GAACTATGTG CCAGGCTTGA CCCCATGTCT TCCTCGTCGG 
GTCCTGGCCA TCAAGGGCCG AGCCACCAGT CTCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_023324949.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023180717.1 Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X1, mRNA.

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CloneID	OXi31288
Clone ID Related Accession (Same CDS sequence)	XM_023324949.1
Accession Version	XM_023324949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	kinesin-like protein KIF21B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036462.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC AGAGCGATTG CTGTGTGAAG GTTGCGTTGA GGATTCGTCC TCAGATGGCC 
AAGGAGAAGA TCGAGGGCTG TCACGTGTGC ACGCTGGTCA CACCTGGAGA ACCGCAGGTC 
CTACTGGGAA AGGACAAGGC CTTCACATAC GACTTTGTGT TCGATATTGA CTCAGAGCAG 
CAGCACATCT ACCAGACCTG TGTGTACAAG CTCATCGAGG GCTGCTTCGA AGGCTACAAC 
GCAACCGTGT TTGCTTATGG ACAGACGGGC TCAGGGAAGA CCTACACCAT GGGGACGGGT 
TTTGATGTGA ATCCGAGCCT GCAGGAGCAG GGCATCATCC CACGGGCCGT TCACCACCTG 
TTTGAGGGCA TCCAGAGCAG GAGGGATCGA GCCCAGGAGA TCGGCATCCA GGCACCAGAG 
TTTAAAGTCA GCGCCCAGTT TTTGGAGTTG TACAATGAGG AGATCCTGGA CTTGTTTGAC 
GGAAGTAGAG ATCCAGAGAA TCGCAGCAGG AAGTCCAACA TCAGAATCCA CGAGGACGCC 
AACGGCAGCA TCTACACCAC AGGAGTCACT TCCAGACTGG TGCAGTCAGA GGACGAGCTG 
CTGCAGTGCC TGAAGCTTGG GGCTCTGTCC CGTACCACAG CCAGCACACA GATGAACGCA 
CAAAGCTCCC GCTCACATGC CATCTTCACC ATCCATCTCT GTCAGATGAG GGTCTTTCAG 
CAGCAGCAAA TGAATGGAGG AGAAGAGAAT GGCGAAGTGA ACGGCGTAGA CTCCTCCCCC 
ATCGCCCAGC CGGAGTACGA GACCCTGATG GCCAAGTTTC ACTTCGTGGA CCTGGCCGGC 
TCAGAAAGGC TCAAACGCAC GGGGGCCACT GGAGAAAGGG CCCGAGAGGG AATCTCTATT 
AACTGTGGAC TGCTGGCTCT TGGAAATGTA ATCAGCGCTT TAGGTGATCA GACTAAGAAA 
GCCGGACACG TCCCATATAG AGACTCCAAA CTTACCCGAC TGTTGCAAGA CTCGCTGGGA 
GGCAACAGCC ACACAGTCAT GATTGCCTGC GTCAGCCCGT CGGACCGGGA CTTCATGGAG 
ACGCTGAACA CACTGAAGTA CGCCAACCGT GCCCGAAACA TCAAGAACAA GGTGGTGGTG 
AACCAAGACA AGACCAGCCA GCAGATCAAC GCCCTACGTG CGGAGGTGGC CCGACTCCAG 
ATGGAGCTGA TGGAGTACAA GGCGGGTAAG CGTGTGGCAG GTGAGGATGG GGCTGAAGGC 
TACAGCGATC TGTACCAGGA GAACGCCATG CTGCAGAGAG AAAACGACTC GCTGCGCCTG 
AGAGTAAAGG CCATGCAGGA AACCATCGAT CATCTCAACA CACGGGTGAC ACATCTGCTG 
GCCAATGAGG TCAGCACTCT GCTCACCAAG TCAAGTGAAG GCAATGAGGA GATTGGAACG 
CTAATACAGA ACTACATCCG AGAAGTTGAA GAACTCAGAT CCAAACTCCT TGAGAGCGAG 
GCCATGAATG AGTCACTACG TCGACAGGTG GCACGGCTCT CCTCTCGTTC CTCGTTTCCA 
GCCTCCGCTC TAAGCCCAGC CCCTGGCCAT CCCCCTGGCT CTTCCCCTAT ATCCATGTCC 
ATGGAGGCCG AGATGACAGA TGTTCTGCGG CGCGCCAAGT TGGACATCGA GCGGCTGAAG 
AAGAAGGAAA GACGGCAGAG AAGGCTGAGC CCAGAGTTGG AGAAAGGTCT CAAGAAACGA 
GTAAAGCTGC ACACTCAGGA AAACGGGGAG AATCGGCGAA GCGGCGAAAA TGGACAGAAT 
GGAGAGATCG ATTCAGATGA CAATTACACC GAGGATATCA TGTCTCCGCT GCAGGAGGAG 
AGTGGCTGTG AGGAAGATGA AGGCGAAGAT GAGGAGGAGG GCAGGGAGGA AGAGGATGAT 
TTTGACAGTG ACGAGAGCCT AGTGGATTCA GATTCTGACT CGGATGAGAA AGCCAACTTC 
CAGGCAGATC TTGCCGACCT AACCTGTGAG ATTGAGATCA AGCAGAAGCT GATCGATGAG 
CTGGAGAACA GCCAGCGGCG GCTGCTGATG TTAAAGCTGC AGTACGAGGA GAAGCTCATC 
CTGCTGCAGA ACAAGATCAG AGACACGCAG CTGGAGAGAG ACCGCGTGCT GCAGAACCTC 
ATGTCCATGG AAAACTACAC TGAGGAGAAG GCCAGCAAGA TCAGGCAGGA GTACGAGAAG 
CGCCTCAAAG AAATGAACCG TGATCTGCTG AAGCTGCAGG CAGCGCAGAA GGAGCATGCA 
CGTCTGCTCA AAAACCAGGG GAGGTACGAG CGGGAACTGA AGAAGCTCCA AACTGAAGTC 
AACGAGATGA AGAAAGCAAA GGTGGTGCTG ATGAAGCAAA TGAAAGAGGA GCAACAGAGA 
AGGAGGATGG TGGAGGCAAA GAGGAATCGA GAGATTGCTC AGCTTAAGAA GGAGCAGCGG 
CGACAGGAGT ACCAGATTCG GGCGCTGGAG TCTCAAAAGA GGCAGCAGGA GCTGGTGCTG 
CGCAGGAAGA CCCAGGAAGT GACGGCCCTG CGGCGCATGG CCAAGCCCAT GTCGGACCGC 
GTGGCGGGTC GAGTTGCCCG CTGGAACCAG ACGCCTCCAG TTACGGACTC CGGAGCTGAT 
TTGTCGGCCA GCACAGCGGC AAGCAGCTCC GAGGCAGAGA CTGCGAGGAC CGTGAGCGGG 
CTGGTGCGCC AGTGGAACAA CAAGAACAAC GCGTATGGAT ACATGGCGGA GAGCGAGGGG 
AGTATGGATG GAACACGGGT GATTAGCAGG AAGAAGCTGC AGAGGAAGCC AGTGGGCCTG 
GGCAGCACCG CACCTGCAGG CAGAGCCAAC AACTTCTCCA AGTCTGCCCG TCTAAAGTGG 
CAAACCCTTG AGCGTCGCAT CATGGACATC GTCATGCAGA GAATGACCAT CTCCAACGTG 
GAAGCAGACA TGGATCGCCT TATCAAGAAA CGAGAAGAGC TGACAATGCA GCAGGAAGCT 
CTCTCACATA AAAGGGAGAT ACTGATGGCC GACGGAGAGG GGCCAGAGGC GGAGGACCGC 
CTCCTTCAGG AAGTTAACGA GGAGATCGAG GTGCTGAATG CCAACATAGA CTACATCAAT 
GACAGTCTGT CGGACTGCCA GGCCACCATC GTGCAGATAG AGGAGACCAA GGATGAACTG 
GACTCGGTGG ATACCTCAGT GGTGATCAGC TCCTGTTCCC TGGCTGAAGC ACGCCACCTG 
CTGGATCACT TCCTGAAGGC TTCCATTGAT AAGAGCTTAC AGGTGGCTCA GAGGGAGGCG 
CAGGTACGAC TGCTCGAGGG TCAGCTGAGG CAGACTGATG TGATCGGCTC GTCTCACAAT 
CACATGATCC TCGACGCGCT GCGAGAAAAA GCAGAGTGCA TCCCTGAGCT CCAGGCTCTC 
ATCCACAACG TCCAGCAAGA AAATGGTTAC GCCAGCACGG ACGAGGAGCC CTCTGAGTTC 
AGCCAAGCCT CTGACAGCAG TGTTTCACAG ATGAAGGAAT CCAACAGCCA GGATGACTTT 
AAAGTAAAGA TGGAGCCTCG CCTCTCGGCC CAGATGAAGG CGGTGTCTGC GGAGTACCTC 
GGTCCCATCC TGGATCCCTC ATCAAGCAGC AAGCAGCAGC ACATCACTAA GTCCCTAGCC 
TCACTGACTG ACATCCAGGA GGATGGCTTA AGTCTCATTC CAGGAAGTCT GGCACTGAGC 
GTTCCCCTCC GGGACCCTTT TCACAGGGAA CGAGCGTCCC GCACAATCAG CCTTCCTGTC 
AGAGGACACA CTTTTCCCAG GCAGTCTCGA GGTTATGACA CTTCCCCCAT TACAAGAAGG 
AAATCTTACG ACCGTTCGTA CAGGCCCACT GATGTCTACA CTCCCCCCTC CTCACCTCCT 
TTAAGGACAA GGAATGATCG CAACGTCTTC TCCAGACTGA CCAGCAACCA AGCGCAAGGT 
TCTGCTCTGG ACAAGTCGGA TGACAGCGAC TCCTCGCTCT CCGAGGTGCT CAGGGGTGTG 
ATCAATCCCA TTGGAGGTGT AAAGGGGGGT CGGACAGCAC CCCTCCAGTG TGTCTCTGTT 
GCCGAGGGCC ACTCGAAGCC GGTTCTGTGT GTGGACGCTA CAGATGAGCT GCTTTTCACT 
GGATCTAAAG ATCGCACCTG TAAGATGTGG AATCTAGTCA CAGGTCAGGA GATTGCCACC 
CTCAAAGGGC ACCCAAACAA CGTGGTGTCG GTAAAATATT GTCCCTCTTC TGGTCTGGTC 
TTCAGCGTCT CCACCTCATA CATCAAGGTG TGGGACATCC GTGACTCTGC AAAGTGCATA 
CGCACACTCA CGTCATCAGG GCAGGTAGTT ACAGGAGACG CGTGCGCTGG CACAACCACC 
CGGACAATCA CCTTTGCTCA GGGCGAGTGT CAGATCAACC AGATAGCGCT CAACCCATTA 
GGATCTGTGC TCTATGCTGC TGCTGGAAAT ATTGTTCGGA TGTGGGACCT TAACAGGATG 
CAGGCAACAG GCAAACTCTC AGGTCACATC GGTTCTGTCA TGTGTCTGAC AGTGGGACAG 
TCTCTGCTGG GCAAAGACCA GGTCATCACT GGCTCCAAAG ACCATTACGT CAAGATATTT 
GATGTGGCAG AAGGAACTCT GGGTAATGTG GGTCCCGCTC ATAACTTTGA GCCTCCGCAC 
TATGACGGCA TTGAATGTTT GGCCGTGCAG GGAGACGTGC TGTTCAGCGG CTCGCGAGAC 
AACGGTATCA AGAAGTGGGA GCTGGAGCAG CAGGAGCTCA CACAGCAAAT CCCGAATGCC 
CACAAGGACT GGGTGTGCGC TCTGGCCTAT GTACCCGGCC GGCCCATGTT GCTGAGCGCC 
TGTCGGGGTG GCATGCTCAA GGTGTGGAAT GTGGAAAACT TTACGCCCAT AGGAGAGGTC 
AGAGGTCATG ACAGCCCCAT TAATGCCATA TGCACCAACT CCAGGCATAT CTTTACAGCC 
TCCAGTGACT GCAGGGTGAA GTTGTGGAAC TATGTGCCAG GCTTGACCCC ATGTCTTCCT 
CGTCGGGTCC TGGCCATCAA GGGCCGAGCC ACCAGTCTCC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023180717.1
CDS	380..5464
Translation	MASQSDCCVKVALRIRPQMAKEKIEGCHVCTLVTPGEPQVLLGKDKAFTYDFVFDIDSEQQHIYQTCVYKLIEGCFEGYNATVFAYGQTGSGKTYTMGTGFDVNPSLQEQGIIPRAVHHLFEGIQSRRDRAQEIGIQAPEFKVSAQFLELYNEEILDLFDGSRDPENRSRKSNIRIHEDANGSIYTTGVTSRLVQSEDELLQCLKLGALSRTTASTQMNAQSSRSHAIFTIHLCQMRVFQQQQMNGGEENGEVNGVDSSPIAQPEYETLMAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQTKKAGHVPYRDSKLTRLLQDSLGGNSHTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQINALRAEVARLQMELMEYKAGKRVAGEDGAEGYSDLYQENAMLQRENDSLRLRVKAMQETIDHLNTRVTHLLANEVSTLLTKSSEGNEEIGTLIQNYIREVEELRSKLLESEAMNESLRRQVARLSSRSSFPASALSPAPGHPPGSSPISMSMEAEMTDVLRRAKLDIERLKKKERRQRRLSPELEKGLKKRVKLHTQENGENRRSGENGQNGEIDSDDNYTEDIMSPLQEESGCEEDEGEDEEEGREEEDDFDSDESLVDSDSDSDEKANFQADLADLTCEIEIKQKLIDELENSQRRLLMLKLQYEEKLILLQNKIRDTQLERDRVLQNLMSMENYTEEKASKIRQEYEKRLKEMNRDLLKLQAAQKEHARLLKNQGRYERELKKLQTEVNEMKKAKVVLMKQMKEEQQRRRMVEAKRNREIAQLKKEQRRQEYQIRALESQKRQQELVLRRKTQEVTALRRMAKPMSDRVAGRVARWNQTPPVTDSGADLSASTAASSSEAETARTVSGLVRQWNNKNNAYGYMAESEGSMDGTRVISRKKLQRKPVGLGSTAPAGRANNFSKSARLKWQTLERRIMDIVMQRMTISNVEADMDRLIKKREELTMQQEALSHKREILMADGEGPEAEDRLLQEVNEEIEVLNANIDYINDSLSDCQATIVQIEETKDELDSVDTSVVISSCSLAEARHLLDHFLKASIDKSLQVAQREAQVRLLEGQLRQTDVIGSSHNHMILDALREKAECIPELQALIHNVQQENGYASTDEEPSEFSQASDSSVSQMKESNSQDDFKVKMEPRLSAQMKAVSAEYLGPILDPSSSSKQQHITKSLASLTDIQEDGLSLIPGSLALSVPLRDPFHRERASRTISLPVRGHTFPRQSRGYDTSPITRRKSYDRSYRPTDVYTPPSSPPLRTRNDRNVFSRLTSNQAQGSALDKSDDSDSSLSEVLRGVINPIGGVKGGRTAPLQCVSVAEGHSKPVLCVDATDELLFTGSKDRTCKMWNLVTGQEIATLKGHPNNVVSVKYCPSSGLVFSVSTSYIKVWDIRDSAKCIRTLTSSGQVVTGDACAGTTTRTITFAQGECQINQIALNPLGSVLYAAAGNIVRMWDLNRMQATGKLSGHIGSVMCLTVGQSLLGKDQVITGSKDHYVKIFDVAEGTLGNVGPAHNFEPPHYDGIECLAVQGDVLFSGSRDNGIKKWELEQQELTQQIPNAHKDWVCALAYVPGRPMLLSACRGGMLKVWNVENFTPIGEVRGHDSPINAICTNSRHIFTASSDCRVKLWNYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_023324949.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023324949.1

ORF Insert Sequence:

ATGGCGAGCC AGAGCGATTG CTGTGTGAAG GTTGCGTTGA GGATTCGTCC TCAGATGGCC 
AAGGAGAAGA TCGAGGGCTG TCACGTGTGC ACGCTGGTCA CACCTGGAGA ACCGCAGGTC 
CTACTGGGAA AGGACAAGGC CTTCACATAC GACTTTGTGT TCGATATTGA CTCAGAGCAG 
CAGCACATCT ACCAGACCTG TGTGTACAAG CTCATCGAGG GCTGCTTCGA AGGCTACAAC 
GCAACCGTGT TTGCTTATGG ACAGACGGGC TCAGGGAAGA CCTACACCAT GGGGACGGGT 
TTTGATGTGA ATCCGAGCCT GCAGGAGCAG GGCATCATCC CACGGGCCGT TCACCACCTG 
TTTGAGGGCA TCCAGAGCAG GAGGGATCGA GCCCAGGAGA TCGGCATCCA GGCACCAGAG 
TTTAAAGTCA GCGCCCAGTT TTTGGAGTTG TACAATGAGG AGATCCTGGA CTTGTTTGAC 
GGAAGTAGAG ATCCAGAGAA TCGCAGCAGG AAGTCCAACA TCAGAATCCA CGAGGACGCC 
AACGGCAGCA TCTACACCAC AGGAGTCACT TCCAGACTGG TGCAGTCAGA GGACGAGCTG 
CTGCAGTGCC TGAAGCTTGG GGCTCTGTCC CGTACCACAG CCAGCACACA GATGAACGCA 
CAAAGCTCCC GCTCACATGC CATCTTCACC ATCCATCTCT GTCAGATGAG GGTCTTTCAG 
CAGCAGCAAA TGAATGGAGG AGAAGAGAAT GGCGAAGTGA ACGGCGTAGA CTCCTCCCCC 
ATCGCCCAGC CGGAGTACGA GACCCTGATG GCCAAGTTTC ACTTCGTGGA CCTGGCCGGC 
TCAGAAAGGC TCAAACGCAC GGGGGCCACT GGAGAAAGGG CCCGAGAGGG AATCTCTATT 
AACTGTGGAC TGCTGGCTCT TGGAAATGTA ATCAGCGCTT TAGGTGATCA GACTAAGAAA 
GCCGGACACG TCCCATATAG AGACTCCAAA CTTACCCGAC TGTTGCAAGA CTCGCTGGGA 
GGCAACAGCC ACACAGTCAT GATTGCCTGC GTCAGCCCGT CGGACCGGGA CTTCATGGAG 
ACGCTGAACA CACTGAAGTA CGCCAACCGT GCCCGAAACA TCAAGAACAA GGTGGTGGTG 
AACCAAGACA AGACCAGCCA GCAGATCAAC GCCCTACGTG CGGAGGTGGC CCGACTCCAG 
ATGGAGCTGA TGGAGTACAA GGCGGGTAAG CGTGTGGCAG GTGAGGATGG GGCTGAAGGC 
TACAGCGATC TGTACCAGGA GAACGCCATG CTGCAGAGAG AAAACGACTC GCTGCGCCTG 
AGAGTAAAGG CCATGCAGGA AACCATCGAT CATCTCAACA CACGGGTGAC ACATCTGCTG 
GCCAATGAGG TCAGCACTCT GCTCACCAAG TCAAGTGAAG GCAATGAGGA GATTGGAACG 
CTAATACAGA ACTACATCCG AGAAGTTGAA GAACTCAGAT CCAAACTCCT TGAGAGCGAG 
GCCATGAATG AGTCACTACG TCGACAGGTG GCACGGCTCT CCTCTCGTTC CTCGTTTCCA 
GCCTCCGCTC TAAGCCCAGC CCCTGGCCAT CCCCCTGGCT CTTCCCCTAT ATCCATGTCC 
ATGGAGGCCG AGATGACAGA TGTTCTGCGG CGCGCCAAGT TGGACATCGA GCGGCTGAAG 
AAGAAGGAAA GACGGCAGAG AAGGCTGAGC CCAGAGTTGG AGAAAGGTCT CAAGAAACGA 
GTAAAGCTGC ACACTCAGGA AAACGGGGAG AATCGGCGAA GCGGCGAAAA TGGACAGAAT 
GGAGAGATCG ATTCAGATGA CAATTACACC GAGGATATCA TGTCTCCGCT GCAGGAGGAG 
AGTGGCTGTG AGGAAGATGA AGGCGAAGAT GAGGAGGAGG GCAGGGAGGA AGAGGATGAT 
TTTGACAGTG ACGAGAGCCT AGTGGATTCA GATTCTGACT CGGATGAGAA AGCCAACTTC 
CAGGCAGATC TTGCCGACCT AACCTGTGAG ATTGAGATCA AGCAGAAGCT GATCGATGAG 
CTGGAGAACA GCCAGCGGCG GCTGCTGATG TTAAAGCTGC AGTACGAGGA GAAGCTCATC 
CTGCTGCAGA ACAAGATCAG AGACACGCAG CTGGAGAGAG ACCGCGTGCT GCAGAACCTC 
ATGTCCATGG AAAACTACAC TGAGGAGAAG GCCAGCAAGA TCAGGCAGGA GTACGAGAAG 
CGCCTCAAAG AAATGAACCG TGATCTGCTG AAGCTGCAGG CAGCGCAGAA GGAGCATGCA 
CGTCTGCTCA AAAACCAGGG GAGGTACGAG CGGGAACTGA AGAAGCTCCA AACTGAAGTC 
AACGAGATGA AGAAAGCAAA GGTGGTGCTG ATGAAGCAAA TGAAAGAGGA GCAACAGAGA 
AGGAGGATGG TGGAGGCAAA GAGGAATCGA GAGATTGCTC AGCTTAAGAA GGAGCAGCGG 
CGACAGGAGT ACCAGATTCG GGCGCTGGAG TCTCAAAAGA GGCAGCAGGA GCTGGTGCTG 
CGCAGGAAGA CCCAGGAAGT GACGGCCCTG CGGCGCATGG CCAAGCCCAT GTCGGACCGC 
GTGGCGGGTC GAGTTGCCCG CTGGAACCAG ACGCCTCCAG TTACGGACTC CGGAGCTGAT 
TTGTCGGCCA GCACAGCGGC AAGCAGCTCC GAGGCAGAGA CTGCGAGGAC CGTGAGCGGG 
CTGGTGCGCC AGTGGAACAA CAAGAACAAC GCGTATGGAT ACATGGCGGA GAGCGAGGGG 
AGTATGGATG GAACACGGGT GATTAGCAGG AAGAAGCTGC AGAGGAAGCC AGTGGGCCTG 
GGCAGCACCG CACCTGCAGG CAGAGCCAAC AACTTCTCCA AGTCTGCCCG TCTAAAGTGG 
CAAACCCTTG AGCGTCGCAT CATGGACATC GTCATGCAGA GAATGACCAT CTCCAACGTG 
GAAGCAGACA TGGATCGCCT TATCAAGAAA CGAGAAGAGC TGACAATGCA GCAGGAAGCT 
CTCTCACATA AAAGGGAGAT ACTGATGGCC GACGGAGAGG GGCCAGAGGC GGAGGACCGC 
CTCCTTCAGG AAGTTAACGA GGAGATCGAG GTGCTGAATG CCAACATAGA CTACATCAAT 
GACAGTCTGT CGGACTGCCA GGCCACCATC GTGCAGATAG AGGAGACCAA GGATGAACTG 
GACTCGGTGG ATACCTCAGT GGTGATCAGC TCCTGTTCCC TGGCTGAAGC ACGCCACCTG 
CTGGATCACT TCCTGAAGGC TTCCATTGAT AAGAGCTTAC AGGTGGCTCA GAGGGAGGCG 
CAGGTACGAC TGCTCGAGGG TCAGCTGAGG CAGACTGATG TGATCGGCTC GTCTCACAAT 
CACATGATCC TCGACGCGCT GCGAGAAAAA GCAGAGTGCA TCCCTGAGCT CCAGGCTCTC 
ATCCACAACG TCCAGCAAGA AAATGGTTAC GCCAGCACGG ACGAGGAGCC CTCTGAGTTC 
AGCCAAGCCT CTGACAGCAG TGTTTCACAG ATGAAGGAAT CCAACAGCCA GGATGACTTT 
AAAGTAAAGA TGGAGCCTCG CCTCTCGGCC CAGATGAAGG CGGTGTCTGC GGAGTACCTC 
GGTCCCATCC TGGATCCCTC ATCAAGCAGC AAGCAGCAGC ACATCACTAA GTCCCTAGCC 
TCACTGACTG ACATCCAGGA GGATGGCTTA AGTCTCATTC CAGGAAGTCT GGCACTGAGC 
GTTCCCCTCC GGGACCCTTT TCACAGGGAA CGAGCGTCCC GCACAATCAG CCTTCCTGTC 
AGAGGACACA CTTTTCCCAG GCAGTCTCGA GGTTATGACA CTTCCCCCAT TACAAGAAGG 
AAATCTTACG ACCGTTCGTA CAGGCCCACT GATGTCTACA CTCCCCCCTC CTCACCTCCT 
TTAAGGACAA GGAATGATCG CAACGTCTTC TCCAGACTGA CCAGCAACCA AGCGCAAGGT 
TCTGCTCTGG ACAAGTCGGA TGACAGCGAC TCCTCGCTCT CCGAGGTGCT CAGGGGTGTG 
ATCAATCCCA TTGGAGGTGT AAAGGGGGGT CGGACAGCAC CCCTCCAGTG TGTCTCTGTT 
GCCGAGGGCC ACTCGAAGCC GGTTCTGTGT GTGGACGCTA CAGATGAGCT GCTTTTCACT 
GGATCTAAAG ATCGCACCTG TAAGATGTGG AATCTAGTCA CAGGTCAGGA GATTGCCACC 
CTCAAAGGGC ACCCAAACAA CGTGGTGTCG GTAAAATATT GTCCCTCTTC TGGTCTGGTC 
TTCAGCGTCT CCACCTCATA CATCAAGGTG TGGGACATCC GTGACTCTGC AAAGTGCATA 
CGCACACTCA CGTCATCAGG GCAGGTAGTT ACAGGAGACG CGTGCGCTGG CACAACCACC 
CGGACAATCA CCTTTGCTCA GGGCGAGTGT CAGATCAACC AGATAGCGCT CAACCCATTA 
GGATCTGTGC TCTATGCTGC TGCTGGAAAT ATTGTTCGGA TGTGGGACCT TAACAGGATG 
CAGGCAACAG GCAAACTCTC AGGTCACATC GGTTCTGTCA TGTGTCTGAC AGTGGGACAG 
TCTCTGCTGG GCAAAGACCA GGTCATCACT GGCTCCAAAG ACCATTACGT CAAGATATTT 
GATGTGGCAG AAGGAACTCT GGGTAATGTG GGTCCCGCTC ATAACTTTGA GCCTCCGCAC 
TATGACGGCA TTGAATGTTT GGCCGTGCAG GGAGACGTGC TGTTCAGCGG CTCGCGAGAC 
AACGGTATCA AGAAGTGGGA GCTGGAGCAG CAGGAGCTCA CACAGCAAAT CCCGAATGCC 
CACAAGGACT GGGTGTGCGC TCTGGCCTAT GTACCCGGCC GGCCCATGTT GCTGAGCGCC 
TGTCGGGGTG GCATGCTCAA GGTGTGGAAT GTGGAAAACT TTACGCCCAT AGGAGAGGTC 
AGAGGTCATG ACAGCCCCAT TAATGCCATA TGCACCAACT CCAGGCATAT CTTTACAGCC 
TCCAGTGACT GCAGGGTGAA GTTGTGGAAC TATGTGCCAG GCTTGACCCC ATGTCTTCCT 
CGTCGGGTCC TGGCCATCAA GGGCCGAGCC ACCAGTCTCC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_023324950.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023180718.1 Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXi31289
Clone ID Related Accession (Same CDS sequence)	XM_023324950.1
Accession Version	XM_023324950.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	kinesin-like protein KIF21B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036462.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC AGAGCGATTG CTGTGTGAAG GTTGCGTTGA GGATTCGTCC TCAGATGGCC 
AAGGAGAAGA TCGAGGGCTG TCACGTGTGC ACGCTGGTCA CACCTGGAGA ACCGCAGGTC 
CTACTGGGAA AGGACAAGGC CTTCACATAC GACTTTGTGT TCGATATTGA CTCAGAGCAG 
CAGCACATCT ACCAGACCTG TGTGTACAAG CTCATCGAGG GCTGCTTCGA AGGCTACAAC 
GCAACCGTGT TTGCTTATGG ACAGACGGGC TCAGGGAAGA CCTACACCAT GGGGACGGGT 
TTTGATGTGA ATCCGAGCCT GCAGGAGCAG GGCATCATCC CACGGGCCGT TCACCACCTG 
TTTGAGGGCA TCCAGAGCAG GAGGGATCGA GCCCAGGAGA TCGGCATCCA GGCACCAGAG 
TTTAAAGTCA GCGCCCAGTT TTTGGAGTTG TACAATGAGG AGATCCTGGA CTTGTTTGAC 
GGAAGTAGAG ATCCAGAGAA TCGCAGCAGG AAGTCCAACA TCAGAATCCA CGAGGACGCC 
AACGGCAGCA TCTACACCAC AGGAGTCACT TCCAGACTGG TGCAGTCAGA GGACGAGCTG 
CTGCAGTGCC TGAAGCTTGG GGCTCTGTCC CGTACCACAG CCAGCACACA GATGAACGCA 
CAAAGCTCCC GCTCACATGC CATCTTCACC ATCCATCTCT GTCAGATGAG GGTCTTTCAG 
CAGCAGCAAA TGAATGGAGG AGAAGAGAAT GGCGAAGTGA ACGGCGTAGA CTCCTCCCCC 
ATCGCCCAGC CGGAGTACGA GACCCTGATG GCCAAGTTTC ACTTCGTGGA CCTGGCCGGC 
TCAGAAAGGC TCAAACGCAC GGGGGCCACT GGAGAAAGGG CCCGAGAGGG AATCTCTATT 
AACTGTGGAC TGCTGGCTCT TGGAAATGTA ATCAGCGCTT TAGGTGATCA GACTAAGAAA 
GCCGGACACG TCCCATATAG AGACTCCAAA CTTACCCGAC TGTTGCAAGA CTCGCTGGGA 
GGCAACAGCC ACACAGTCAT GATTGCCTGC GTCAGCCCGT CGGACCGGGA CTTCATGGAG 
ACGCTGAACA CACTGAAGTA CGCCAACCGT GCCCGAAACA TCAAGAACAA GGTGGTGGTG 
AACCAAGACA AGACCAGCCA GCAGATCAAC GCCCTACGTG CGGAGGTGGC CCGACTCCAG 
ATGGAGCTGA TGGAGTACAA GGCGGGTAAG CGTGTGGCAG GTGAGGATGG GGCTGAAGGC 
TACAGCGATC TGTACCAGGA GAACGCCATG CTGCAGAGAG AAAACGACTC GCTGCGCCTG 
AGAGTAAAGG CCATGCAGGA AACCATCGAT CATCTCAACA CACGGGTGAC ACATCTGCTG 
GCCAATGAGG TCAGCACTCT GCTCACCAAG TCAAGTGAAG GCAATGAGGA GATTGGAACG 
CTAATACAGA ACTACATCCG AGAAGTTGAA GAACTCAGAT CCAAACTCCT TGAGAGCGAG 
GCCATGAATG AGTCACTACG TCGACAGGTG GCACGGCTCT CCTCTCGTTC CTCGTTTCCA 
GCCTCCGCTC TAAGCCCAGC CCCTGGCCAT CCCCCTGGCT CTTCCCCTAT ATCCATGTCC 
ATGGAGGCCG AGATGACAGA TGTTCTGCGG CGCGCCAAGT TGGACATCGA GCGGCTGAAG 
AAGAAGGAAA GACGGCAGAG AAGGCTGAGC CCAGAGTTGG AGAAAGGTCT CAAGAAACGA 
GTAAAGCTGC ACACTCAGGA AAACGGGGAG AATCGGCGAA GCGGCGAAAA TGGACAGAAT 
GGAGAGATCG ATTCAGATGA CAATTACACC GAGGATATCA TGTCTCCGCT GCAGGAGGAG 
AGTGGCTGTG AGGAAGATGA AGGCGAAGAT GAGGAGGAGG GCAGGGAGGA AGAGGATGAT 
TTTGACAGTG ACGAGAGCCT AGTGGATTCA GATTCTGACT CGGATGAGAA AGCCAACTTC 
CAGGCAGATC TTGCCGACCT AACCTGTGAG ATTGAGATCA AGCAGAAGCT GATCGATGAG 
CTGGAGAACA GCCAGCGGCG GCTGCTGATG TTAAAGCTGC AGTACGAGGA GAAGCTCATC 
CTGCTGCAGA ACAAGATCAG AGACACGCAG CTGGAGAGAG ACCGCGTGCT GCAGAACCTC 
ATGTCCATGG AAAACTACAC TGAGGAGAAG GCCAGCAAGA TCAGGCAGGA GTACGAGAAG 
CGCCTCAAAG AAATGAACCG TGATCTGCTG AAGCTGCAGG CAGCGCAGAA GGAGCATGCA 
CGTCTGCTCA AAAACCAGGG GAGGTACGAG CGGGAACTGA AGAAGCTCCA AACTGAAGTC 
AACGAGATGA AGAAAGCAAA GGTGGTGCTG ATGAAGCAAA TGAAAGAGGA GCAACAGAGA 
AGGAGGATGG TGGAGGCAAA GAGGAATCGA GAGATTGCTC AGCTTAAGAA GGAGCAGCGG 
CGACAGGAGT ACCAGATTCG GGCGCTGGAG TCTCAAAAGA GGCAGCAGGA GCTGGTGCTG 
CGCAGGAAGA CCCAGGAAGT GACGGCCCTG CGGCGCATGG CCAAGCCCAT GTCGGACCGC 
GTGGCGGGTC GAGTTGCCCG CTGGAACCAG ACGCCTCCAG TTACGGACTC CGGAGCTGAT 
TTGTCGGCCA GCACAGCGGC AAGCAGCTCC GAGGCAGAGA CTGCGAGGAC CGTGAGCGGG 
CTGGTGCGCC AGTGGAACAA CAAGAACAAC GCGTATGGAT ACATGGCGGA GAGCGAGGGG 
AGTATGGATG GAACACGGGT GATTAGGAAG AAGCTGCAGA GGAAGCCAGT GGGCCTGGGC 
AGCACCGCAC CTGCAGGCAG AGCCAACAAC TTCTCCAAGT CTGCCCGTCT AAAGTGGCAA 
ACCCTTGAGC GTCGCATCAT GGACATCGTC ATGCAGAGAA TGACCATCTC CAACGTGGAA 
GCAGACATGG ATCGCCTTAT CAAGAAACGA GAAGAGCTGA CAATGCAGCA GGAAGCTCTC 
TCACATAAAA GGGAGATACT GATGGCCGAC GGAGAGGGGC CAGAGGCGGA GGACCGCCTC 
CTTCAGGAAG TTAACGAGGA GATCGAGGTG CTGAATGCCA ACATAGACTA CATCAATGAC 
AGTCTGTCGG ACTGCCAGGC CACCATCGTG CAGATAGAGG AGACCAAGGA TGAACTGGAC 
TCGGTGGATA CCTCAGTGGT GATCAGCTCC TGTTCCCTGG CTGAAGCACG CCACCTGCTG 
GATCACTTCC TGAAGGCTTC CATTGATAAG AGCTTACAGG TGGCTCAGAG GGAGGCGCAG 
GTACGACTGC TCGAGGGTCA GCTGAGGCAG ACTGATGTGA TCGGCTCGTC TCACAATCAC 
ATGATCCTCG ACGCGCTGCG AGAAAAAGCA GAGTGCATCC CTGAGCTCCA GGCTCTCATC 
CACAACGTCC AGCAAGAAAA TGGTTACGCC AGCACGGACG AGGAGCCCTC TGAGTTCAGC 
CAAGCCTCTG ACAGCAGTGT TTCACAGATG AAGGAATCCA ACAGCCAGGA TGACTTTAAA 
GTAAAGATGG AGCCTCGCCT CTCGGCCCAG ATGAAGGCGG TGTCTGCGGA GTACCTCGGT 
CCCATCCTGG ATCCCTCATC AAGCAGCAAG CAGCAGCACA TCACTAAGTC CCTAGCCTCA 
CTGACTGACA TCCAGGAGGA TGGCTTAAGT CTCATTCCAG GAAGTCTGGC ACTGAGCGTT 
CCCCTCCGGG ACCCTTTTCA CAGGGAACGA GCGTCCCGCA CAATCAGCCT TCCTGTCAGA 
GGACACACTT TTCCCAGGCA GTCTCGAGGT TATGACACTT CCCCCATTAC AAGAAGGAAA 
TCTTACGACC GTTCGTACAG GCCCACTGAT GTCTACACTC CCCCCTCCTC ACCTCCTTTA 
AGGACAAGGA ATGATCGCAA CGTCTTCTCC AGACTGACCA GCAACCAAGC GCAAGGTTCT 
GCTCTGGACA AGTCGGATGA CAGCGACTCC TCGCTCTCCG AGGTGCTCAG GGGTGTGATC 
AATCCCATTG GAGGTGTAAA GGGGGGTCGG ACAGCACCCC TCCAGTGTGT CTCTGTTGCC 
GAGGGCCACT CGAAGCCGGT TCTGTGTGTG GACGCTACAG ATGAGCTGCT TTTCACTGGA 
TCTAAAGATC GCACCTGTAA GATGTGGAAT CTAGTCACAG GTCAGGAGAT TGCCACCCTC 
AAAGGGCACC CAAACAACGT GGTGTCGGTA AAATATTGTC CCTCTTCTGG TCTGGTCTTC 
AGCGTCTCCA CCTCATACAT CAAGGTGTGG GACATCCGTG ACTCTGCAAA GTGCATACGC 
ACACTCACGT CATCAGGGCA GGTAGTTACA GGAGACGCGT GCGCTGGCAC AACCACCCGG 
ACAATCACCT TTGCTCAGGG CGAGTGTCAG ATCAACCAGA TAGCGCTCAA CCCATTAGGA 
TCTGTGCTCT ATGCTGCTGC TGGAAATATT GTTCGGATGT GGGACCTTAA CAGGATGCAG 
GCAACAGGCA AACTCTCAGG TCACATCGGT TCTGTCATGT GTCTGACAGT GGGACAGTCT 
CTGCTGGGCA AAGACCAGGT CATCACTGGC TCCAAAGACC ATTACGTCAA GATATTTGAT 
GTGGCAGAAG GAACTCTGGG TAATGTGGGT CCCGCTCATA ACTTTGAGCC TCCGCACTAT 
GACGGCATTG AATGTTTGGC CGTGCAGGGA GACGTGCTGT TCAGCGGCTC GCGAGACAAC 
GGTATCAAGA AGTGGGAGCT GGAGCAGCAG GAGCTCACAC AGCAAATCCC GAATGCCCAC 
AAGGACTGGG TGTGCGCTCT GGCCTATGTA CCCGGCCGGC CCATGTTGCT GAGCGCCTGT 
CGGGGTGGCA TGCTCAAGGT GTGGAATGTG GAAAACTTTA CGCCCATAGG AGAGGTCAGA 
GGTCATGACA GCCCCATTAA TGCCATATGC ACCAACTCCA GGCATATCTT TACAGCCTCC 
AGTGACTGCA GGGTGAAGTT GTGGAACTAT GTGCCAGGCT TGACCCCATG TCTTCCTCGT 
CGGGTCCTGG CCATCAAGGG CCGAGCCACC AGTCTCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023180718.1
CDS	380..5461
Translation	MASQSDCCVKVALRIRPQMAKEKIEGCHVCTLVTPGEPQVLLGKDKAFTYDFVFDIDSEQQHIYQTCVYKLIEGCFEGYNATVFAYGQTGSGKTYTMGTGFDVNPSLQEQGIIPRAVHHLFEGIQSRRDRAQEIGIQAPEFKVSAQFLELYNEEILDLFDGSRDPENRSRKSNIRIHEDANGSIYTTGVTSRLVQSEDELLQCLKLGALSRTTASTQMNAQSSRSHAIFTIHLCQMRVFQQQQMNGGEENGEVNGVDSSPIAQPEYETLMAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQTKKAGHVPYRDSKLTRLLQDSLGGNSHTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQINALRAEVARLQMELMEYKAGKRVAGEDGAEGYSDLYQENAMLQRENDSLRLRVKAMQETIDHLNTRVTHLLANEVSTLLTKSSEGNEEIGTLIQNYIREVEELRSKLLESEAMNESLRRQVARLSSRSSFPASALSPAPGHPPGSSPISMSMEAEMTDVLRRAKLDIERLKKKERRQRRLSPELEKGLKKRVKLHTQENGENRRSGENGQNGEIDSDDNYTEDIMSPLQEESGCEEDEGEDEEEGREEEDDFDSDESLVDSDSDSDEKANFQADLADLTCEIEIKQKLIDELENSQRRLLMLKLQYEEKLILLQNKIRDTQLERDRVLQNLMSMENYTEEKASKIRQEYEKRLKEMNRDLLKLQAAQKEHARLLKNQGRYERELKKLQTEVNEMKKAKVVLMKQMKEEQQRRRMVEAKRNREIAQLKKEQRRQEYQIRALESQKRQQELVLRRKTQEVTALRRMAKPMSDRVAGRVARWNQTPPVTDSGADLSASTAASSSEAETARTVSGLVRQWNNKNNAYGYMAESEGSMDGTRVIRKKLQRKPVGLGSTAPAGRANNFSKSARLKWQTLERRIMDIVMQRMTISNVEADMDRLIKKREELTMQQEALSHKREILMADGEGPEAEDRLLQEVNEEIEVLNANIDYINDSLSDCQATIVQIEETKDELDSVDTSVVISSCSLAEARHLLDHFLKASIDKSLQVAQREAQVRLLEGQLRQTDVIGSSHNHMILDALREKAECIPELQALIHNVQQENGYASTDEEPSEFSQASDSSVSQMKESNSQDDFKVKMEPRLSAQMKAVSAEYLGPILDPSSSSKQQHITKSLASLTDIQEDGLSLIPGSLALSVPLRDPFHRERASRTISLPVRGHTFPRQSRGYDTSPITRRKSYDRSYRPTDVYTPPSSPPLRTRNDRNVFSRLTSNQAQGSALDKSDDSDSSLSEVLRGVINPIGGVKGGRTAPLQCVSVAEGHSKPVLCVDATDELLFTGSKDRTCKMWNLVTGQEIATLKGHPNNVVSVKYCPSSGLVFSVSTSYIKVWDIRDSAKCIRTLTSSGQVVTGDACAGTTTRTITFAQGECQINQIALNPLGSVLYAAAGNIVRMWDLNRMQATGKLSGHIGSVMCLTVGQSLLGKDQVITGSKDHYVKIFDVAEGTLGNVGPAHNFEPPHYDGIECLAVQGDVLFSGSRDNGIKKWELEQQELTQQIPNAHKDWVCALAYVPGRPMLLSACRGGMLKVWNVENFTPIGEVRGHDSPINAICTNSRHIFTASSDCRVKLWNYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_023324950.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023324950.1

ORF Insert Sequence:

ATGGCGAGCC AGAGCGATTG CTGTGTGAAG GTTGCGTTGA GGATTCGTCC TCAGATGGCC 
AAGGAGAAGA TCGAGGGCTG TCACGTGTGC ACGCTGGTCA CACCTGGAGA ACCGCAGGTC 
CTACTGGGAA AGGACAAGGC CTTCACATAC GACTTTGTGT TCGATATTGA CTCAGAGCAG 
CAGCACATCT ACCAGACCTG TGTGTACAAG CTCATCGAGG GCTGCTTCGA AGGCTACAAC 
GCAACCGTGT TTGCTTATGG ACAGACGGGC TCAGGGAAGA CCTACACCAT GGGGACGGGT 
TTTGATGTGA ATCCGAGCCT GCAGGAGCAG GGCATCATCC CACGGGCCGT TCACCACCTG 
TTTGAGGGCA TCCAGAGCAG GAGGGATCGA GCCCAGGAGA TCGGCATCCA GGCACCAGAG 
TTTAAAGTCA GCGCCCAGTT TTTGGAGTTG TACAATGAGG AGATCCTGGA CTTGTTTGAC 
GGAAGTAGAG ATCCAGAGAA TCGCAGCAGG AAGTCCAACA TCAGAATCCA CGAGGACGCC 
AACGGCAGCA TCTACACCAC AGGAGTCACT TCCAGACTGG TGCAGTCAGA GGACGAGCTG 
CTGCAGTGCC TGAAGCTTGG GGCTCTGTCC CGTACCACAG CCAGCACACA GATGAACGCA 
CAAAGCTCCC GCTCACATGC CATCTTCACC ATCCATCTCT GTCAGATGAG GGTCTTTCAG 
CAGCAGCAAA TGAATGGAGG AGAAGAGAAT GGCGAAGTGA ACGGCGTAGA CTCCTCCCCC 
ATCGCCCAGC CGGAGTACGA GACCCTGATG GCCAAGTTTC ACTTCGTGGA CCTGGCCGGC 
TCAGAAAGGC TCAAACGCAC GGGGGCCACT GGAGAAAGGG CCCGAGAGGG AATCTCTATT 
AACTGTGGAC TGCTGGCTCT TGGAAATGTA ATCAGCGCTT TAGGTGATCA GACTAAGAAA 
GCCGGACACG TCCCATATAG AGACTCCAAA CTTACCCGAC TGTTGCAAGA CTCGCTGGGA 
GGCAACAGCC ACACAGTCAT GATTGCCTGC GTCAGCCCGT CGGACCGGGA CTTCATGGAG 
ACGCTGAACA CACTGAAGTA CGCCAACCGT GCCCGAAACA TCAAGAACAA GGTGGTGGTG 
AACCAAGACA AGACCAGCCA GCAGATCAAC GCCCTACGTG CGGAGGTGGC CCGACTCCAG 
ATGGAGCTGA TGGAGTACAA GGCGGGTAAG CGTGTGGCAG GTGAGGATGG GGCTGAAGGC 
TACAGCGATC TGTACCAGGA GAACGCCATG CTGCAGAGAG AAAACGACTC GCTGCGCCTG 
AGAGTAAAGG CCATGCAGGA AACCATCGAT CATCTCAACA CACGGGTGAC ACATCTGCTG 
GCCAATGAGG TCAGCACTCT GCTCACCAAG TCAAGTGAAG GCAATGAGGA GATTGGAACG 
CTAATACAGA ACTACATCCG AGAAGTTGAA GAACTCAGAT CCAAACTCCT TGAGAGCGAG 
GCCATGAATG AGTCACTACG TCGACAGGTG GCACGGCTCT CCTCTCGTTC CTCGTTTCCA 
GCCTCCGCTC TAAGCCCAGC CCCTGGCCAT CCCCCTGGCT CTTCCCCTAT ATCCATGTCC 
ATGGAGGCCG AGATGACAGA TGTTCTGCGG CGCGCCAAGT TGGACATCGA GCGGCTGAAG 
AAGAAGGAAA GACGGCAGAG AAGGCTGAGC CCAGAGTTGG AGAAAGGTCT CAAGAAACGA 
GTAAAGCTGC ACACTCAGGA AAACGGGGAG AATCGGCGAA GCGGCGAAAA TGGACAGAAT 
GGAGAGATCG ATTCAGATGA CAATTACACC GAGGATATCA TGTCTCCGCT GCAGGAGGAG 
AGTGGCTGTG AGGAAGATGA AGGCGAAGAT GAGGAGGAGG GCAGGGAGGA AGAGGATGAT 
TTTGACAGTG ACGAGAGCCT AGTGGATTCA GATTCTGACT CGGATGAGAA AGCCAACTTC 
CAGGCAGATC TTGCCGACCT AACCTGTGAG ATTGAGATCA AGCAGAAGCT GATCGATGAG 
CTGGAGAACA GCCAGCGGCG GCTGCTGATG TTAAAGCTGC AGTACGAGGA GAAGCTCATC 
CTGCTGCAGA ACAAGATCAG AGACACGCAG CTGGAGAGAG ACCGCGTGCT GCAGAACCTC 
ATGTCCATGG AAAACTACAC TGAGGAGAAG GCCAGCAAGA TCAGGCAGGA GTACGAGAAG 
CGCCTCAAAG AAATGAACCG TGATCTGCTG AAGCTGCAGG CAGCGCAGAA GGAGCATGCA 
CGTCTGCTCA AAAACCAGGG GAGGTACGAG CGGGAACTGA AGAAGCTCCA AACTGAAGTC 
AACGAGATGA AGAAAGCAAA GGTGGTGCTG ATGAAGCAAA TGAAAGAGGA GCAACAGAGA 
AGGAGGATGG TGGAGGCAAA GAGGAATCGA GAGATTGCTC AGCTTAAGAA GGAGCAGCGG 
CGACAGGAGT ACCAGATTCG GGCGCTGGAG TCTCAAAAGA GGCAGCAGGA GCTGGTGCTG 
CGCAGGAAGA CCCAGGAAGT GACGGCCCTG CGGCGCATGG CCAAGCCCAT GTCGGACCGC 
GTGGCGGGTC GAGTTGCCCG CTGGAACCAG ACGCCTCCAG TTACGGACTC CGGAGCTGAT 
TTGTCGGCCA GCACAGCGGC AAGCAGCTCC GAGGCAGAGA CTGCGAGGAC CGTGAGCGGG 
CTGGTGCGCC AGTGGAACAA CAAGAACAAC GCGTATGGAT ACATGGCGGA GAGCGAGGGG 
AGTATGGATG GAACACGGGT GATTAGGAAG AAGCTGCAGA GGAAGCCAGT GGGCCTGGGC 
AGCACCGCAC CTGCAGGCAG AGCCAACAAC TTCTCCAAGT CTGCCCGTCT AAAGTGGCAA 
ACCCTTGAGC GTCGCATCAT GGACATCGTC ATGCAGAGAA TGACCATCTC CAACGTGGAA 
GCAGACATGG ATCGCCTTAT CAAGAAACGA GAAGAGCTGA CAATGCAGCA GGAAGCTCTC 
TCACATAAAA GGGAGATACT GATGGCCGAC GGAGAGGGGC CAGAGGCGGA GGACCGCCTC 
CTTCAGGAAG TTAACGAGGA GATCGAGGTG CTGAATGCCA ACATAGACTA CATCAATGAC 
AGTCTGTCGG ACTGCCAGGC CACCATCGTG CAGATAGAGG AGACCAAGGA TGAACTGGAC 
TCGGTGGATA CCTCAGTGGT GATCAGCTCC TGTTCCCTGG CTGAAGCACG CCACCTGCTG 
GATCACTTCC TGAAGGCTTC CATTGATAAG AGCTTACAGG TGGCTCAGAG GGAGGCGCAG 
GTACGACTGC TCGAGGGTCA GCTGAGGCAG ACTGATGTGA TCGGCTCGTC TCACAATCAC 
ATGATCCTCG ACGCGCTGCG AGAAAAAGCA GAGTGCATCC CTGAGCTCCA GGCTCTCATC 
CACAACGTCC AGCAAGAAAA TGGTTACGCC AGCACGGACG AGGAGCCCTC TGAGTTCAGC 
CAAGCCTCTG ACAGCAGTGT TTCACAGATG AAGGAATCCA ACAGCCAGGA TGACTTTAAA 
GTAAAGATGG AGCCTCGCCT CTCGGCCCAG ATGAAGGCGG TGTCTGCGGA GTACCTCGGT 
CCCATCCTGG ATCCCTCATC AAGCAGCAAG CAGCAGCACA TCACTAAGTC CCTAGCCTCA 
CTGACTGACA TCCAGGAGGA TGGCTTAAGT CTCATTCCAG GAAGTCTGGC ACTGAGCGTT 
CCCCTCCGGG ACCCTTTTCA CAGGGAACGA GCGTCCCGCA CAATCAGCCT TCCTGTCAGA 
GGACACACTT TTCCCAGGCA GTCTCGAGGT TATGACACTT CCCCCATTAC AAGAAGGAAA 
TCTTACGACC GTTCGTACAG GCCCACTGAT GTCTACACTC CCCCCTCCTC ACCTCCTTTA 
AGGACAAGGA ATGATCGCAA CGTCTTCTCC AGACTGACCA GCAACCAAGC GCAAGGTTCT 
GCTCTGGACA AGTCGGATGA CAGCGACTCC TCGCTCTCCG AGGTGCTCAG GGGTGTGATC 
AATCCCATTG GAGGTGTAAA GGGGGGTCGG ACAGCACCCC TCCAGTGTGT CTCTGTTGCC 
GAGGGCCACT CGAAGCCGGT TCTGTGTGTG GACGCTACAG ATGAGCTGCT TTTCACTGGA 
TCTAAAGATC GCACCTGTAA GATGTGGAAT CTAGTCACAG GTCAGGAGAT TGCCACCCTC 
AAAGGGCACC CAAACAACGT GGTGTCGGTA AAATATTGTC CCTCTTCTGG TCTGGTCTTC 
AGCGTCTCCA CCTCATACAT CAAGGTGTGG GACATCCGTG ACTCTGCAAA GTGCATACGC 
ACACTCACGT CATCAGGGCA GGTAGTTACA GGAGACGCGT GCGCTGGCAC AACCACCCGG 
ACAATCACCT TTGCTCAGGG CGAGTGTCAG ATCAACCAGA TAGCGCTCAA CCCATTAGGA 
TCTGTGCTCT ATGCTGCTGC TGGAAATATT GTTCGGATGT GGGACCTTAA CAGGATGCAG 
GCAACAGGCA AACTCTCAGG TCACATCGGT TCTGTCATGT GTCTGACAGT GGGACAGTCT 
CTGCTGGGCA AAGACCAGGT CATCACTGGC TCCAAAGACC ATTACGTCAA GATATTTGAT 
GTGGCAGAAG GAACTCTGGG TAATGTGGGT CCCGCTCATA ACTTTGAGCC TCCGCACTAT 
GACGGCATTG AATGTTTGGC CGTGCAGGGA GACGTGCTGT TCAGCGGCTC GCGAGACAAC 
GGTATCAAGA AGTGGGAGCT GGAGCAGCAG GAGCTCACAC AGCAAATCCC GAATGCCCAC 
AAGGACTGGG TGTGCGCTCT GGCCTATGTA CCCGGCCGGC CCATGTTGCT GAGCGCCTGT 
CGGGGTGGCA TGCTCAAGGT GTGGAATGTG GAAAACTTTA CGCCCATAGG AGAGGTCAGA 
GGTCATGACA GCCCCATTAA TGCCATATGC ACCAACTCCA GGCATATCTT TACAGCCTCC 
AGTGACTGCA GGGTGAAGTT GTGGAACTAT GTGCCAGGCT TGACCCCATG TCTTCCTCGT 
CGGGTCCTGG CCATCAAGGG CCGAGCCACC AGTCTCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_023324951.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023180719.1 Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X3, mRNA.

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CloneID	OXi31290
Clone ID Related Accession (Same CDS sequence)	XM_023324951.1
Accession Version	XM_023324951.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	kinesin-like protein KIF21B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036462.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC AGAGCGATTG CTGTGTGAAG GTTGCGTTGA GGATTCGTCC TCAGATGGCC 
AAGGAGAAGA TCGAGGGCTG TCACGTGTGC ACGCTGGTCA CACCTGGAGA ACCGCAGGTC 
CTACTGGGAA AGGACAAGGC CTTCACATAC GACTTTGTGT TCGATATTGA CTCAGAGCAG 
CAGCACATCT ACCAGACCTG TGTGTACAAG CTCATCGAGG GCTGCTTCGA AGGCTACAAC 
GCAACCGTGT TTGCTTATGG ACAGACGGGC TCAGGGAAGA CCTACACCAT GGGGACGGGT 
TTTGATGTGA ATCCGAGCCT GCAGGAGCAG GGCATCATCC CACGGGCCGT TCACCACCTG 
TTTGAGGGCA TCCAGAGCAG GAGGGATCGA GCCCAGGAGA TCGGCATCCA GGCACCAGAG 
TTTAAAGTCA GCGCCCAGTT TTTGGAGTTG TACAATGAGG AGATCCTGGA CTTGTTTGAC 
GGAAGTAGAG ATCCAGAGAA TCGCAGCAGG AAGTCCAACA TCAGAATCCA CGAGGACGCC 
AACGGCAGCA TCTACACCAC AGGAGTCACT TCCAGACTGG TGCAGTCAGA GGACGAGCTG 
CTGCAGTGCC TGAAGCTTGG GGCTCTGTCC CGTACCACAG CCAGCACACA GATGAACGCA 
CAAAGCTCCC GCTCACATGC CATCTTCACC ATCCATCTCT GTCAGATGAG GGTCTTTCAG 
CAGCAGCAAA TGAATGGAGG AGAAGAGAAT GGCGAAGTGA ACGGCGTAGA CTCCTCCCCC 
ATCGCCCAGC CGGAGTACGA GACCCTGATG GCCAAGTTTC ACTTCGTGGA CCTGGCCGGC 
TCAGAAAGGC TCAAACGCAC GGGGGCCACT GGAGAAAGGG CCCGAGAGGG AATCTCTATT 
AACTGTGGAC TGCTGGCTCT TGGAAATGTA ATCAGCGCTT TAGGTGATCA GACTAAGAAA 
GCCGGACACG TCCCATATAG AGACTCCAAA CTTACCCGAC TGTTGCAAGA CTCGCTGGGA 
GGCAACAGCC ACACAGTCAT GATTGCCTGC GTCAGCCCGT CGGACCGGGA CTTCATGGAG 
ACGCTGAACA CACTGAAGTA CGCCAACCGT GCCCGAAACA TCAAGAACAA GGTGGTGGTG 
AACCAAGACA AGACCAGCCA GCAGATCAAC GCCCTACGTG CGGAGGTGGC CCGACTCCAG 
ATGGAGCTGA TGGAGTACAA GGCGGGTAAG CGTGTGGCAG GTGAGGATGG GGCTGAAGGC 
TACAGCGATC TGTACCAGGA GAACGCCATG CTGCAGAGAG AAAACGACTC GCTGCGCCTG 
AGAGTAAAGG CCATGCAGGA AACCATCGAT CATCTCAACA CACGGGTGAC ACATCTGCTG 
GCCAATGAGG TCAGCACTCT GCTCACCAAG TCAAGTGAAG GCAATGAGGA GATTGGAACG 
CTAATACAGA ACTACATCCG AGAAGTTGAA GAACTCAGAT CCAAACTCCT TGAGAGCGAG 
GCCATGAATG AGTCACTACG TCGACAGGTG GCACGGCTCT CCTCTCGTTC CTCGTTTCCA 
GCCTCCGCTC TAAGCCCAGC CCCTGGCCAT CCCCCTGGCT CTTCCCCTAT ATCCATGTCC 
ATGGAGGCCG AGATGACAGA TGTTCTGCGG CGCGCCAAGT TGGACATCGA GCGGCTGAAG 
AAGAAGGAAA GACGGCAGAG AAGGCTGAGC CCAGAGTTGG AGAAAGGTCT CAAGAAACGA 
GTAAAGCTGC ACACTCAGGA AAACGGGGAG AATCGGCGAA GCGGCGAAAA TGGACAGAAT 
GGAGAGATCG ATTCAGATGA CAATTACACC GAGGATATCA TGTCTCCGCT GCAGGAGGAG 
AGTGGCTGTG AGGAAGATGA AGGCGAAGAT GAGGAGGAGG GCAGGGAGGA AGAGGATGAT 
TTTGACAGTG ACGAGAGCCT AGTGGATTCA GATTCTGACT CGGATGAGAA AGCCAACTTC 
CAGGCAGATC TTGCCGACCT AACCTGTGAG ATTGAGATCA AGCAGAAGCT GATCGATGAG 
CTGGAGAACA GCCAGCGGCG GCTGCTGATG TTAAAGCTGC AGTACGAGGA GAAGCTCATC 
CTGCTGCAGA ACAAGATCAG AGACACGCAG CTGGAGAGAG ACCGCGTGCT GCAGAACCTC 
ATGTCCATGG AAAACTACAC TGAGGAGAAG GCCAGCAAGA TCAGGCAGGA GTACGAGAAG 
CGCCTCAAAG AAATGAACCG TGATCTGCTG AAGCTGCAGG CAGCGCAGAA GGAGCATGCA 
CGTCTGCTCA AAAACCAGGG GAGGTACGAG CGGGAACTGA AGAAGCTCCA AACTGAAGTC 
AACGAGATGA AGAAAGCAAA GGTGGTGCTG ATGAAGCAAA TGAAAGAGGA GCAACAGAGA 
AGGAGGATGG TGGAGGCAAA GAGGAATCGA GAGATTGCTC AGCTTAAGAA GGAGCAGCGG 
CGACAGGAGT ACCAGATTCG GGCGCTGGAG TCTCAAAAGA GGCAGCAGGA GCTGGTGCTG 
CGCAGGAAGA CCCAGGAAGT GACGGCCCTG CGGCGCATGG CCAAGCCCAT GTCGGACCGC 
GTGGCGGGTC GAGTTGCCCG CTGGAACCAG ACGCCTCCAG TTACGGACTC CGGAGCTGAT 
TTGTCGGCCA GCACAGCGGC AAGCAGCTCC GAGGCAGAGA CTGCGAGGAC CGTGAGCGGG 
CTGGTGCGCC AGTGGAACAA CAAGAACAAC GCGTATGGAT ACATGGCGGA GAGCGAGGGG 
AGTATGGATG GAACACGGGT GATTAGCAGG AAGAAGCTGC AGAGGAAGCC AGTGGGCCTG 
GGCAGCACCG CACCTGCAGG CAGAGCCAAC AACTTCTCCA AGTCTGCCCG TCTAAAGTGG 
CAAACCCTTG AGCGTCGCAT CATGGACATC GTCATGCAGA GAATGACCAT CTCCAACGTG 
GAAGCAGACA TGGATCGCCT TATCAAGAAA CGAGAAGAGC TGACAATGCA GCAGGAAGCT 
CTCTCACATA AAAGGGAGAT ACTGATGGCC GACGGAGAGG GGCCAGAGGC GGAGGACCGC 
CTCCTTCAGG AAGTTAACGA GGAGATCGAG GTGCTGAATG CCAACATAGA CTACATCAAT 
GACAGTCTGT CGGACTGCCA GGCCACCATC GTGCAGATAG AGGAGACCAA GGATGAACTG 
GACTCGGTGG ATACCTCAGT GGTGATCAGC TCCTGTTCCC TGGCTGAAGC ACGCCACCTG 
CTGGATCACT TCCTGAAGGC TTCCATTGAT AAGAGCTTAC AGGTGGCTCA GAGGGAGGCG 
CAGGTACGAC TGCTCGAGGG TCAGCTGAGG CAGACTGATG TGATCGGCTC GTCTCACAAT 
CACATGATCC TCGACGCGCT GCGAGAAAAA GCAGAGTGCA TCCCTGAGCT CCAGGCTCTC 
ATCCACAACG TCCAGCAAGA AAATGGTTAC GCCAGCACGG ACGAGGAGCC CTCTGAGTTC 
AGCCAAGCCT CTGACAGCAG TGTTTCACAG ATGAAGGAAT CCAACAGCCA GGATGACTTT 
AAAGTAAAGA TGGAGCCTCG CCTCTCGGCC CAGATGAAGG CGGTGTCTGC GGAGTACCTC 
GGTCCCATCC TGGATCCCTC ATCAAGCAGC AAGCAGCAGC ACATCACTAA GTCCCTAGCC 
TCACTGACTG ACATCCAGGA GGATGGCTTA AGTCTCATTC CAGGAAGTCT GGCACTGAGC 
GTTCCCCTCC GGGACCCTTT TCACAGGGAA CGAGCGTCCC GCACAATCAG CCTTCCTGTC 
AGAGGACACA CTTTTCCCAG GCAGTCTCGA GGTTATGACA CTTCCCCCAT TACAAGAAGG 
AAATCTTACG ACCGTTCGTA CAGGCCCACT GATGTCTACA CTCCCCCCTC CTCACCTCCT 
TTAAGGACAA GGAATGATCG CAACGTCTTC TCCAGACTGA CCAGCAACCA AGCGCAAGGT 
TCTGCTCTGG ACAAGGGTGT GATCAATCCC ATTGGAGGTG TAAAGGGGGG TCGGACAGCA 
CCCCTCCAGT GTGTCTCTGT TGCCGAGGGC CACTCGAAGC CGGTTCTGTG TGTGGACGCT 
ACAGATGAGC TGCTTTTCAC TGGATCTAAA GATCGCACCT GTAAGATGTG GAATCTAGTC 
ACAGGTCAGG AGATTGCCAC CCTCAAAGGG CACCCAAACA ACGTGGTGTC GGTAAAATAT 
TGTCCCTCTT CTGGTCTGGT CTTCAGCGTC TCCACCTCAT ACATCAAGGT GTGGGACATC 
CGTGACTCTG CAAAGTGCAT ACGCACACTC ACGTCATCAG GGCAGGTAGT TACAGGAGAC 
GCGTGCGCTG GCACAACCAC CCGGACAATC ACCTTTGCTC AGGGCGAGTG TCAGATCAAC 
CAGATAGCGC TCAACCCATT AGGATCTGTG CTCTATGCTG CTGCTGGAAA TATTGTTCGG 
ATGTGGGACC TTAACAGGAT GCAGGCAACA GGCAAACTCT CAGGTCACAT CGGTTCTGTC 
ATGTGTCTGA CAGTGGGACA GTCTCTGCTG GGCAAAGACC AGGTCATCAC TGGCTCCAAA 
GACCATTACG TCAAGATATT TGATGTGGCA GAAGGAACTC TGGGTAATGT GGGTCCCGCT 
CATAACTTTG AGCCTCCGCA CTATGACGGC ATTGAATGTT TGGCCGTGCA GGGAGACGTG 
CTGTTCAGCG GCTCGCGAGA CAACGGTATC AAGAAGTGGG AGCTGGAGCA GCAGGAGCTC 
ACACAGCAAA TCCCGAATGC CCACAAGGAC TGGGTGTGCG CTCTGGCCTA TGTACCCGGC 
CGGCCCATGT TGCTGAGCGC CTGTCGGGGT GGCATGCTCA AGGTGTGGAA TGTGGAAAAC 
TTTACGCCCA TAGGAGAGGT CAGAGGTCAT GACAGCCCCA TTAATGCCAT ATGCACCAAC 
TCCAGGCATA TCTTTACAGC CTCCAGTGAC TGCAGGGTGA AGTTGTGGAA CTATGTGCCA 
GGCTTGACCC CATGTCTTCC TCGTCGGGTC CTGGCCATCA AGGGCCGAGC CACCAGTCTC 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023180719.1
CDS	380..5425
Translation	MASQSDCCVKVALRIRPQMAKEKIEGCHVCTLVTPGEPQVLLGKDKAFTYDFVFDIDSEQQHIYQTCVYKLIEGCFEGYNATVFAYGQTGSGKTYTMGTGFDVNPSLQEQGIIPRAVHHLFEGIQSRRDRAQEIGIQAPEFKVSAQFLELYNEEILDLFDGSRDPENRSRKSNIRIHEDANGSIYTTGVTSRLVQSEDELLQCLKLGALSRTTASTQMNAQSSRSHAIFTIHLCQMRVFQQQQMNGGEENGEVNGVDSSPIAQPEYETLMAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQTKKAGHVPYRDSKLTRLLQDSLGGNSHTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQINALRAEVARLQMELMEYKAGKRVAGEDGAEGYSDLYQENAMLQRENDSLRLRVKAMQETIDHLNTRVTHLLANEVSTLLTKSSEGNEEIGTLIQNYIREVEELRSKLLESEAMNESLRRQVARLSSRSSFPASALSPAPGHPPGSSPISMSMEAEMTDVLRRAKLDIERLKKKERRQRRLSPELEKGLKKRVKLHTQENGENRRSGENGQNGEIDSDDNYTEDIMSPLQEESGCEEDEGEDEEEGREEEDDFDSDESLVDSDSDSDEKANFQADLADLTCEIEIKQKLIDELENSQRRLLMLKLQYEEKLILLQNKIRDTQLERDRVLQNLMSMENYTEEKASKIRQEYEKRLKEMNRDLLKLQAAQKEHARLLKNQGRYERELKKLQTEVNEMKKAKVVLMKQMKEEQQRRRMVEAKRNREIAQLKKEQRRQEYQIRALESQKRQQELVLRRKTQEVTALRRMAKPMSDRVAGRVARWNQTPPVTDSGADLSASTAASSSEAETARTVSGLVRQWNNKNNAYGYMAESEGSMDGTRVISRKKLQRKPVGLGSTAPAGRANNFSKSARLKWQTLERRIMDIVMQRMTISNVEADMDRLIKKREELTMQQEALSHKREILMADGEGPEAEDRLLQEVNEEIEVLNANIDYINDSLSDCQATIVQIEETKDELDSVDTSVVISSCSLAEARHLLDHFLKASIDKSLQVAQREAQVRLLEGQLRQTDVIGSSHNHMILDALREKAECIPELQALIHNVQQENGYASTDEEPSEFSQASDSSVSQMKESNSQDDFKVKMEPRLSAQMKAVSAEYLGPILDPSSSSKQQHITKSLASLTDIQEDGLSLIPGSLALSVPLRDPFHRERASRTISLPVRGHTFPRQSRGYDTSPITRRKSYDRSYRPTDVYTPPSSPPLRTRNDRNVFSRLTSNQAQGSALDKGVINPIGGVKGGRTAPLQCVSVAEGHSKPVLCVDATDELLFTGSKDRTCKMWNLVTGQEIATLKGHPNNVVSVKYCPSSGLVFSVSTSYIKVWDIRDSAKCIRTLTSSGQVVTGDACAGTTTRTITFAQGECQINQIALNPLGSVLYAAAGNIVRMWDLNRMQATGKLSGHIGSVMCLTVGQSLLGKDQVITGSKDHYVKIFDVAEGTLGNVGPAHNFEPPHYDGIECLAVQGDVLFSGSRDNGIKKWELEQQELTQQIPNAHKDWVCALAYVPGRPMLLSACRGGMLKVWNVENFTPIGEVRGHDSPINAICTNSRHIFTASSDCRVKLWNYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_023324951.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023324951.1

ORF Insert Sequence:

ATGGCGAGCC AGAGCGATTG CTGTGTGAAG GTTGCGTTGA GGATTCGTCC TCAGATGGCC 
AAGGAGAAGA TCGAGGGCTG TCACGTGTGC ACGCTGGTCA CACCTGGAGA ACCGCAGGTC 
CTACTGGGAA AGGACAAGGC CTTCACATAC GACTTTGTGT TCGATATTGA CTCAGAGCAG 
CAGCACATCT ACCAGACCTG TGTGTACAAG CTCATCGAGG GCTGCTTCGA AGGCTACAAC 
GCAACCGTGT TTGCTTATGG ACAGACGGGC TCAGGGAAGA CCTACACCAT GGGGACGGGT 
TTTGATGTGA ATCCGAGCCT GCAGGAGCAG GGCATCATCC CACGGGCCGT TCACCACCTG 
TTTGAGGGCA TCCAGAGCAG GAGGGATCGA GCCCAGGAGA TCGGCATCCA GGCACCAGAG 
TTTAAAGTCA GCGCCCAGTT TTTGGAGTTG TACAATGAGG AGATCCTGGA CTTGTTTGAC 
GGAAGTAGAG ATCCAGAGAA TCGCAGCAGG AAGTCCAACA TCAGAATCCA CGAGGACGCC 
AACGGCAGCA TCTACACCAC AGGAGTCACT TCCAGACTGG TGCAGTCAGA GGACGAGCTG 
CTGCAGTGCC TGAAGCTTGG GGCTCTGTCC CGTACCACAG CCAGCACACA GATGAACGCA 
CAAAGCTCCC GCTCACATGC CATCTTCACC ATCCATCTCT GTCAGATGAG GGTCTTTCAG 
CAGCAGCAAA TGAATGGAGG AGAAGAGAAT GGCGAAGTGA ACGGCGTAGA CTCCTCCCCC 
ATCGCCCAGC CGGAGTACGA GACCCTGATG GCCAAGTTTC ACTTCGTGGA CCTGGCCGGC 
TCAGAAAGGC TCAAACGCAC GGGGGCCACT GGAGAAAGGG CCCGAGAGGG AATCTCTATT 
AACTGTGGAC TGCTGGCTCT TGGAAATGTA ATCAGCGCTT TAGGTGATCA GACTAAGAAA 
GCCGGACACG TCCCATATAG AGACTCCAAA CTTACCCGAC TGTTGCAAGA CTCGCTGGGA 
GGCAACAGCC ACACAGTCAT GATTGCCTGC GTCAGCCCGT CGGACCGGGA CTTCATGGAG 
ACGCTGAACA CACTGAAGTA CGCCAACCGT GCCCGAAACA TCAAGAACAA GGTGGTGGTG 
AACCAAGACA AGACCAGCCA GCAGATCAAC GCCCTACGTG CGGAGGTGGC CCGACTCCAG 
ATGGAGCTGA TGGAGTACAA GGCGGGTAAG CGTGTGGCAG GTGAGGATGG GGCTGAAGGC 
TACAGCGATC TGTACCAGGA GAACGCCATG CTGCAGAGAG AAAACGACTC GCTGCGCCTG 
AGAGTAAAGG CCATGCAGGA AACCATCGAT CATCTCAACA CACGGGTGAC ACATCTGCTG 
GCCAATGAGG TCAGCACTCT GCTCACCAAG TCAAGTGAAG GCAATGAGGA GATTGGAACG 
CTAATACAGA ACTACATCCG AGAAGTTGAA GAACTCAGAT CCAAACTCCT TGAGAGCGAG 
GCCATGAATG AGTCACTACG TCGACAGGTG GCACGGCTCT CCTCTCGTTC CTCGTTTCCA 
GCCTCCGCTC TAAGCCCAGC CCCTGGCCAT CCCCCTGGCT CTTCCCCTAT ATCCATGTCC 
ATGGAGGCCG AGATGACAGA TGTTCTGCGG CGCGCCAAGT TGGACATCGA GCGGCTGAAG 
AAGAAGGAAA GACGGCAGAG AAGGCTGAGC CCAGAGTTGG AGAAAGGTCT CAAGAAACGA 
GTAAAGCTGC ACACTCAGGA AAACGGGGAG AATCGGCGAA GCGGCGAAAA TGGACAGAAT 
GGAGAGATCG ATTCAGATGA CAATTACACC GAGGATATCA TGTCTCCGCT GCAGGAGGAG 
AGTGGCTGTG AGGAAGATGA AGGCGAAGAT GAGGAGGAGG GCAGGGAGGA AGAGGATGAT 
TTTGACAGTG ACGAGAGCCT AGTGGATTCA GATTCTGACT CGGATGAGAA AGCCAACTTC 
CAGGCAGATC TTGCCGACCT AACCTGTGAG ATTGAGATCA AGCAGAAGCT GATCGATGAG 
CTGGAGAACA GCCAGCGGCG GCTGCTGATG TTAAAGCTGC AGTACGAGGA GAAGCTCATC 
CTGCTGCAGA ACAAGATCAG AGACACGCAG CTGGAGAGAG ACCGCGTGCT GCAGAACCTC 
ATGTCCATGG AAAACTACAC TGAGGAGAAG GCCAGCAAGA TCAGGCAGGA GTACGAGAAG 
CGCCTCAAAG AAATGAACCG TGATCTGCTG AAGCTGCAGG CAGCGCAGAA GGAGCATGCA 
CGTCTGCTCA AAAACCAGGG GAGGTACGAG CGGGAACTGA AGAAGCTCCA AACTGAAGTC 
AACGAGATGA AGAAAGCAAA GGTGGTGCTG ATGAAGCAAA TGAAAGAGGA GCAACAGAGA 
AGGAGGATGG TGGAGGCAAA GAGGAATCGA GAGATTGCTC AGCTTAAGAA GGAGCAGCGG 
CGACAGGAGT ACCAGATTCG GGCGCTGGAG TCTCAAAAGA GGCAGCAGGA GCTGGTGCTG 
CGCAGGAAGA CCCAGGAAGT GACGGCCCTG CGGCGCATGG CCAAGCCCAT GTCGGACCGC 
GTGGCGGGTC GAGTTGCCCG CTGGAACCAG ACGCCTCCAG TTACGGACTC CGGAGCTGAT 
TTGTCGGCCA GCACAGCGGC AAGCAGCTCC GAGGCAGAGA CTGCGAGGAC CGTGAGCGGG 
CTGGTGCGCC AGTGGAACAA CAAGAACAAC GCGTATGGAT ACATGGCGGA GAGCGAGGGG 
AGTATGGATG GAACACGGGT GATTAGCAGG AAGAAGCTGC AGAGGAAGCC AGTGGGCCTG 
GGCAGCACCG CACCTGCAGG CAGAGCCAAC AACTTCTCCA AGTCTGCCCG TCTAAAGTGG 
CAAACCCTTG AGCGTCGCAT CATGGACATC GTCATGCAGA GAATGACCAT CTCCAACGTG 
GAAGCAGACA TGGATCGCCT TATCAAGAAA CGAGAAGAGC TGACAATGCA GCAGGAAGCT 
CTCTCACATA AAAGGGAGAT ACTGATGGCC GACGGAGAGG GGCCAGAGGC GGAGGACCGC 
CTCCTTCAGG AAGTTAACGA GGAGATCGAG GTGCTGAATG CCAACATAGA CTACATCAAT 
GACAGTCTGT CGGACTGCCA GGCCACCATC GTGCAGATAG AGGAGACCAA GGATGAACTG 
GACTCGGTGG ATACCTCAGT GGTGATCAGC TCCTGTTCCC TGGCTGAAGC ACGCCACCTG 
CTGGATCACT TCCTGAAGGC TTCCATTGAT AAGAGCTTAC AGGTGGCTCA GAGGGAGGCG 
CAGGTACGAC TGCTCGAGGG TCAGCTGAGG CAGACTGATG TGATCGGCTC GTCTCACAAT 
CACATGATCC TCGACGCGCT GCGAGAAAAA GCAGAGTGCA TCCCTGAGCT CCAGGCTCTC 
ATCCACAACG TCCAGCAAGA AAATGGTTAC GCCAGCACGG ACGAGGAGCC CTCTGAGTTC 
AGCCAAGCCT CTGACAGCAG TGTTTCACAG ATGAAGGAAT CCAACAGCCA GGATGACTTT 
AAAGTAAAGA TGGAGCCTCG CCTCTCGGCC CAGATGAAGG CGGTGTCTGC GGAGTACCTC 
GGTCCCATCC TGGATCCCTC ATCAAGCAGC AAGCAGCAGC ACATCACTAA GTCCCTAGCC 
TCACTGACTG ACATCCAGGA GGATGGCTTA AGTCTCATTC CAGGAAGTCT GGCACTGAGC 
GTTCCCCTCC GGGACCCTTT TCACAGGGAA CGAGCGTCCC GCACAATCAG CCTTCCTGTC 
AGAGGACACA CTTTTCCCAG GCAGTCTCGA GGTTATGACA CTTCCCCCAT TACAAGAAGG 
AAATCTTACG ACCGTTCGTA CAGGCCCACT GATGTCTACA CTCCCCCCTC CTCACCTCCT 
TTAAGGACAA GGAATGATCG CAACGTCTTC TCCAGACTGA CCAGCAACCA AGCGCAAGGT 
TCTGCTCTGG ACAAGGGTGT GATCAATCCC ATTGGAGGTG TAAAGGGGGG TCGGACAGCA 
CCCCTCCAGT GTGTCTCTGT TGCCGAGGGC CACTCGAAGC CGGTTCTGTG TGTGGACGCT 
ACAGATGAGC TGCTTTTCAC TGGATCTAAA GATCGCACCT GTAAGATGTG GAATCTAGTC 
ACAGGTCAGG AGATTGCCAC CCTCAAAGGG CACCCAAACA ACGTGGTGTC GGTAAAATAT 
TGTCCCTCTT CTGGTCTGGT CTTCAGCGTC TCCACCTCAT ACATCAAGGT GTGGGACATC 
CGTGACTCTG CAAAGTGCAT ACGCACACTC ACGTCATCAG GGCAGGTAGT TACAGGAGAC 
GCGTGCGCTG GCACAACCAC CCGGACAATC ACCTTTGCTC AGGGCGAGTG TCAGATCAAC 
CAGATAGCGC TCAACCCATT AGGATCTGTG CTCTATGCTG CTGCTGGAAA TATTGTTCGG 
ATGTGGGACC TTAACAGGAT GCAGGCAACA GGCAAACTCT CAGGTCACAT CGGTTCTGTC 
ATGTGTCTGA CAGTGGGACA GTCTCTGCTG GGCAAAGACC AGGTCATCAC TGGCTCCAAA 
GACCATTACG TCAAGATATT TGATGTGGCA GAAGGAACTC TGGGTAATGT GGGTCCCGCT 
CATAACTTTG AGCCTCCGCA CTATGACGGC ATTGAATGTT TGGCCGTGCA GGGAGACGTG 
CTGTTCAGCG GCTCGCGAGA CAACGGTATC AAGAAGTGGG AGCTGGAGCA GCAGGAGCTC 
ACACAGCAAA TCCCGAATGC CCACAAGGAC TGGGTGTGCG CTCTGGCCTA TGTACCCGGC 
CGGCCCATGT TGCTGAGCGC CTGTCGGGGT GGCATGCTCA AGGTGTGGAA TGTGGAAAAC 
TTTACGCCCA TAGGAGAGGT CAGAGGTCAT GACAGCCCCA TTAATGCCAT ATGCACCAAC 
TCCAGGCATA TCTTTACAGC CTCCAGTGAC TGCAGGGTGA AGTTGTGGAA CTATGTGCCA 
GGCTTGACCC CATGTCTTCC TCGTCGGGTC CTGGCCATCA AGGGCCGAGC CACCAGTCTC 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_023324952.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023180720.1 Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X4, mRNA.

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CloneID	OXi31291
Clone ID Related Accession (Same CDS sequence)	XM_023324952.1
Accession Version	XM_023324952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	kinesin-like protein KIF21B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036462.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC AGAGCGATTG CTGTGTGAAG GTTGCGTTGA GGATTCGTCC TCAGATGGCC 
AAGGAGAAGA TCGAGGGCTG TCACGTGTGC ACGCTGGTCA CACCTGGAGA ACCGCAGGTC 
CTACTGGGAA AGGACAAGGC CTTCACATAC GACTTTGTGT TCGATATTGA CTCAGAGCAG 
CAGCACATCT ACCAGACCTG TGTGTACAAG CTCATCGAGG GCTGCTTCGA AGGCTACAAC 
GCAACCGTGT TTGCTTATGG ACAGACGGGC TCAGGGAAGA CCTACACCAT GGGGACGGGT 
TTTGATGTGA ATCCGAGCCT GCAGGAGCAG GGCATCATCC CACGGGCCGT TCACCACCTG 
TTTGAGGGCA TCCAGAGCAG GAGGGATCGA GCCCAGGAGA TCGGCATCCA GGCACCAGAG 
TTTAAAGTCA GCGCCCAGTT TTTGGAGTTG TACAATGAGG AGATCCTGGA CTTGTTTGAC 
GGAAGTAGAG ATCCAGAGAA TCGCAGCAGG AAGTCCAACA TCAGAATCCA CGAGGACGCC 
AACGGCAGCA TCTACACCAC AGGAGTCACT TCCAGACTGG TGCAGTCAGA GGACGAGCTG 
CTGCAGTGCC TGAAGCTTGG GGCTCTGTCC CGTACCACAG CCAGCACACA GATGAACGCA 
CAAAGCTCCC GCTCACATGC CATCTTCACC ATCCATCTCT GTCAGATGAG GGTCTTTCAG 
CAGCAGCAAA TGAATGGAGG AGAAGAGAAT GGCGAAGTGA ACGGCGTAGA CTCCTCCCCC 
ATCGCCCAGC CGGAGTACGA GACCCTGATG GCCAAGTTTC ACTTCGTGGA CCTGGCCGGC 
TCAGAAAGGC TCAAACGCAC GGGGGCCACT GGAGAAAGGG CCCGAGAGGG AATCTCTATT 
AACTGTGGAC TGCTGGCTCT TGGAAATGTA ATCAGCGCTT TAGGTGATCA GACTAAGAAA 
GCCGGACACG TCCCATATAG AGACTCCAAA CTTACCCGAC TGTTGCAAGA CTCGCTGGGA 
GGCAACAGCC ACACAGTCAT GATTGCCTGC GTCAGCCCGT CGGACCGGGA CTTCATGGAG 
ACGCTGAACA CACTGAAGTA CGCCAACCGT GCCCGAAACA TCAAGAACAA GGTGGTGGTG 
AACCAAGACA AGACCAGCCA GCAGATCAAC GCCCTACGTG CGGAGGTGGC CCGACTCCAG 
ATGGAGCTGA TGGAGTACAA GGCGGGTAAG CGTGTGGCAG GTGAGGATGG GGCTGAAGGC 
TACAGCGATC TGTACCAGGA GAACGCCATG CTGCAGAGAG AAAACGACTC GCTGCGCCTG 
AGAGTAAAGG CCATGCAGGA AACCATCGAT CATCTCAACA CACGGGTGAC ACATCTGCTG 
GCCAATGAGG TCAGCACTCT GCTCACCAAG TCAAGTGAAG GCAATGAGGA GATTGGAACG 
CTAATACAGA ACTACATCCG AGAAGTTGAA GAACTCAGAT CCAAACTCCT TGAGAGCGAG 
GCCATGAATG AGTCACTACG TCGACAGGTG GCACGGCTCT CCTCTCGTTC CTCGTTTCCA 
GCCTCCGCTC TAAGCCCAGC CCCTGGCCAT CCCCCTGGCT CTTCCCCTAT ATCCATGTCC 
ATGGAGGCCG AGATGACAGA TGTTCTGCGG CGCGCCAAGT TGGACATCGA GCGGCTGAAG 
AAGAAGGAAA GACGGCAGAG AAGGCTGAGC CCAGAGTTGG AGAAAGGTCT CAAGAAACGA 
GTAAAGCTGC ACACTCAGGA AAACGGGGAG AATCGGCGAA GCGGCGAAAA TGGACAGAAT 
GGAGAGATCG ATTCAGATGA CAATTACACC GAGGATATCA TGTCTCCGCT GCAGGAGGAG 
AGTGGCTGTG AGGAAGATGA AGGCGAAGAT GAGGAGGAGG GCAGGGAGGA AGAGGATGAT 
TTTGACAGTG ACGAGAGCCT AGTGGATTCA GATTCTGACT CGGATGAGAA AGCCAACTTC 
CAGGCAGATC TTGCCGACCT AACCTGTGAG ATTGAGATCA AGCAGAAGCT GATCGATGAG 
CTGGAGAACA GCCAGCGGCG GCTGCTGATG TTAAAGCTGC AGTACGAGGA GAAGCTCATC 
CTGCTGCAGA ACAAGATCAG AGACACGCAG CTGGAGAGAG ACCGCGTGCT GCAGAACCTC 
ATGTCCATGG AAAACTACAC TGAGGAGAAG GCCAGCAAGA TCAGGCAGGA GTACGAGAAG 
CGCCTCAAAG AAATGAACCG TGATCTGCTG AAGCTGCAGG CAGCGCAGAA GGAGCATGCA 
CGTCTGCTCA AAAACCAGGG GAGGTACGAG CGGGAACTGA AGAAGCTCCA AACTGAAGTC 
AACGAGATGA AGAAAGCAAA GGTGGTGCTG ATGAAGCAAA TGAAAGAGGA GCAACAGAGA 
AGGAGGATGG TGGAGGCAAA GAGGAATCGA GAGATTGCTC AGCTTAAGAA GGAGCAGCGG 
CGACAGGAGT ACCAGATTCG GGCGCTGGAG TCTCAAAAGA GGCAGCAGGA GCTGGTGCTG 
CGCAGGAAGA CCCAGGAAGT GACGGCCCTG CGGCGCATGG CCAAGCCCAT GTCGGACCGC 
GTGGCGGGTC GAGTTGCCCG CTGGAACCAG ACGCCTCCAG TTACGGACTC CGGAGCTGAT 
TTGTCGGCCA GCACAGCGGC AAGCAGCTCC GAGGCAGAGA CTGCGAGGAC CGTGAGCGGG 
CTGGTGCGCC AGTGGAACAA CAAGAACAAC GCGTATGGAT ACATGGCGGA GAGCGAGGGG 
AGTATGGATG GAACACGGGT GATTAGGAAG AAGCTGCAGA GGAAGCCAGT GGGCCTGGGC 
AGCACCGCAC CTGCAGGCAG AGCCAACAAC TTCTCCAAGT CTGCCCGTCT AAAGTGGCAA 
ACCCTTGAGC GTCGCATCAT GGACATCGTC ATGCAGAGAA TGACCATCTC CAACGTGGAA 
GCAGACATGG ATCGCCTTAT CAAGAAACGA GAAGAGCTGA CAATGCAGCA GGAAGCTCTC 
TCACATAAAA GGGAGATACT GATGGCCGAC GGAGAGGGGC CAGAGGCGGA GGACCGCCTC 
CTTCAGGAAG TTAACGAGGA GATCGAGGTG CTGAATGCCA ACATAGACTA CATCAATGAC 
AGTCTGTCGG ACTGCCAGGC CACCATCGTG CAGATAGAGG AGACCAAGGA TGAACTGGAC 
TCGGTGGATA CCTCAGTGGT GATCAGCTCC TGTTCCCTGG CTGAAGCACG CCACCTGCTG 
GATCACTTCC TGAAGGCTTC CATTGATAAG AGCTTACAGG TGGCTCAGAG GGAGGCGCAG 
GTACGACTGC TCGAGGGTCA GCTGAGGCAG ACTGATGTGA TCGGCTCGTC TCACAATCAC 
ATGATCCTCG ACGCGCTGCG AGAAAAAGCA GAGTGCATCC CTGAGCTCCA GGCTCTCATC 
CACAACGTCC AGCAAGAAAA TGGTTACGCC AGCACGGACG AGGAGCCCTC TGAGTTCAGC 
CAAGCCTCTG ACAGCAGTGT TTCACAGATG AAGGAATCCA ACAGCCAGGA TGACTTTAAA 
GTAAAGATGG AGCCTCGCCT CTCGGCCCAG ATGAAGGCGG TGTCTGCGGA GTACCTCGGT 
CCCATCCTGG ATCCCTCATC AAGCAGCAAG CAGCAGCACA TCACTAAGTC CCTAGCCTCA 
CTGACTGACA TCCAGGAGGA TGGCTTAAGT CTCATTCCAG GAAGTCTGGC ACTGAGCGTT 
CCCCTCCGGG ACCCTTTTCA CAGGGAACGA GCGTCCCGCA CAATCAGCCT TCCTGTCAGA 
GGACACACTT TTCCCAGGCA GTCTCGAGGT TATGACACTT CCCCCATTAC AAGAAGGAAA 
TCTTACGACC GTTCGTACAG GCCCACTGAT GTCTACACTC CCCCCTCCTC ACCTCCTTTA 
AGGACAAGGA ATGATCGCAA CGTCTTCTCC AGACTGACCA GCAACCAAGC GCAAGGTTCT 
GCTCTGGACA AGGGTGTGAT CAATCCCATT GGAGGTGTAA AGGGGGGTCG GACAGCACCC 
CTCCAGTGTG TCTCTGTTGC CGAGGGCCAC TCGAAGCCGG TTCTGTGTGT GGACGCTACA 
GATGAGCTGC TTTTCACTGG ATCTAAAGAT CGCACCTGTA AGATGTGGAA TCTAGTCACA 
GGTCAGGAGA TTGCCACCCT CAAAGGGCAC CCAAACAACG TGGTGTCGGT AAAATATTGT 
CCCTCTTCTG GTCTGGTCTT CAGCGTCTCC ACCTCATACA TCAAGGTGTG GGACATCCGT 
GACTCTGCAA AGTGCATACG CACACTCACG TCATCAGGGC AGGTAGTTAC AGGAGACGCG 
TGCGCTGGCA CAACCACCCG GACAATCACC TTTGCTCAGG GCGAGTGTCA GATCAACCAG 
ATAGCGCTCA ACCCATTAGG ATCTGTGCTC TATGCTGCTG CTGGAAATAT TGTTCGGATG 
TGGGACCTTA ACAGGATGCA GGCAACAGGC AAACTCTCAG GTCACATCGG TTCTGTCATG 
TGTCTGACAG TGGGACAGTC TCTGCTGGGC AAAGACCAGG TCATCACTGG CTCCAAAGAC 
CATTACGTCA AGATATTTGA TGTGGCAGAA GGAACTCTGG GTAATGTGGG TCCCGCTCAT 
AACTTTGAGC CTCCGCACTA TGACGGCATT GAATGTTTGG CCGTGCAGGG AGACGTGCTG 
TTCAGCGGCT CGCGAGACAA CGGTATCAAG AAGTGGGAGC TGGAGCAGCA GGAGCTCACA 
CAGCAAATCC CGAATGCCCA CAAGGACTGG GTGTGCGCTC TGGCCTATGT ACCCGGCCGG 
CCCATGTTGC TGAGCGCCTG TCGGGGTGGC ATGCTCAAGG TGTGGAATGT GGAAAACTTT 
ACGCCCATAG GAGAGGTCAG AGGTCATGAC AGCCCCATTA ATGCCATATG CACCAACTCC 
AGGCATATCT TTACAGCCTC CAGTGACTGC AGGGTGAAGT TGTGGAACTA TGTGCCAGGC 
TTGACCCCAT GTCTTCCTCG TCGGGTCCTG GCCATCAAGG GCCGAGCCAC CAGTCTCCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023180720.1
CDS	380..5422
Translation	MASQSDCCVKVALRIRPQMAKEKIEGCHVCTLVTPGEPQVLLGKDKAFTYDFVFDIDSEQQHIYQTCVYKLIEGCFEGYNATVFAYGQTGSGKTYTMGTGFDVNPSLQEQGIIPRAVHHLFEGIQSRRDRAQEIGIQAPEFKVSAQFLELYNEEILDLFDGSRDPENRSRKSNIRIHEDANGSIYTTGVTSRLVQSEDELLQCLKLGALSRTTASTQMNAQSSRSHAIFTIHLCQMRVFQQQQMNGGEENGEVNGVDSSPIAQPEYETLMAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQTKKAGHVPYRDSKLTRLLQDSLGGNSHTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQINALRAEVARLQMELMEYKAGKRVAGEDGAEGYSDLYQENAMLQRENDSLRLRVKAMQETIDHLNTRVTHLLANEVSTLLTKSSEGNEEIGTLIQNYIREVEELRSKLLESEAMNESLRRQVARLSSRSSFPASALSPAPGHPPGSSPISMSMEAEMTDVLRRAKLDIERLKKKERRQRRLSPELEKGLKKRVKLHTQENGENRRSGENGQNGEIDSDDNYTEDIMSPLQEESGCEEDEGEDEEEGREEEDDFDSDESLVDSDSDSDEKANFQADLADLTCEIEIKQKLIDELENSQRRLLMLKLQYEEKLILLQNKIRDTQLERDRVLQNLMSMENYTEEKASKIRQEYEKRLKEMNRDLLKLQAAQKEHARLLKNQGRYERELKKLQTEVNEMKKAKVVLMKQMKEEQQRRRMVEAKRNREIAQLKKEQRRQEYQIRALESQKRQQELVLRRKTQEVTALRRMAKPMSDRVAGRVARWNQTPPVTDSGADLSASTAASSSEAETARTVSGLVRQWNNKNNAYGYMAESEGSMDGTRVIRKKLQRKPVGLGSTAPAGRANNFSKSARLKWQTLERRIMDIVMQRMTISNVEADMDRLIKKREELTMQQEALSHKREILMADGEGPEAEDRLLQEVNEEIEVLNANIDYINDSLSDCQATIVQIEETKDELDSVDTSVVISSCSLAEARHLLDHFLKASIDKSLQVAQREAQVRLLEGQLRQTDVIGSSHNHMILDALREKAECIPELQALIHNVQQENGYASTDEEPSEFSQASDSSVSQMKESNSQDDFKVKMEPRLSAQMKAVSAEYLGPILDPSSSSKQQHITKSLASLTDIQEDGLSLIPGSLALSVPLRDPFHRERASRTISLPVRGHTFPRQSRGYDTSPITRRKSYDRSYRPTDVYTPPSSPPLRTRNDRNVFSRLTSNQAQGSALDKGVINPIGGVKGGRTAPLQCVSVAEGHSKPVLCVDATDELLFTGSKDRTCKMWNLVTGQEIATLKGHPNNVVSVKYCPSSGLVFSVSTSYIKVWDIRDSAKCIRTLTSSGQVVTGDACAGTTTRTITFAQGECQINQIALNPLGSVLYAAAGNIVRMWDLNRMQATGKLSGHIGSVMCLTVGQSLLGKDQVITGSKDHYVKIFDVAEGTLGNVGPAHNFEPPHYDGIECLAVQGDVLFSGSRDNGIKKWELEQQELTQQIPNAHKDWVCALAYVPGRPMLLSACRGGMLKVWNVENFTPIGEVRGHDSPINAICTNSRHIFTASSDCRVKLWNYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_023324952.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023324952.1

ORF Insert Sequence:

ATGGCGAGCC AGAGCGATTG CTGTGTGAAG GTTGCGTTGA GGATTCGTCC TCAGATGGCC 
AAGGAGAAGA TCGAGGGCTG TCACGTGTGC ACGCTGGTCA CACCTGGAGA ACCGCAGGTC 
CTACTGGGAA AGGACAAGGC CTTCACATAC GACTTTGTGT TCGATATTGA CTCAGAGCAG 
CAGCACATCT ACCAGACCTG TGTGTACAAG CTCATCGAGG GCTGCTTCGA AGGCTACAAC 
GCAACCGTGT TTGCTTATGG ACAGACGGGC TCAGGGAAGA CCTACACCAT GGGGACGGGT 
TTTGATGTGA ATCCGAGCCT GCAGGAGCAG GGCATCATCC CACGGGCCGT TCACCACCTG 
TTTGAGGGCA TCCAGAGCAG GAGGGATCGA GCCCAGGAGA TCGGCATCCA GGCACCAGAG 
TTTAAAGTCA GCGCCCAGTT TTTGGAGTTG TACAATGAGG AGATCCTGGA CTTGTTTGAC 
GGAAGTAGAG ATCCAGAGAA TCGCAGCAGG AAGTCCAACA TCAGAATCCA CGAGGACGCC 
AACGGCAGCA TCTACACCAC AGGAGTCACT TCCAGACTGG TGCAGTCAGA GGACGAGCTG 
CTGCAGTGCC TGAAGCTTGG GGCTCTGTCC CGTACCACAG CCAGCACACA GATGAACGCA 
CAAAGCTCCC GCTCACATGC CATCTTCACC ATCCATCTCT GTCAGATGAG GGTCTTTCAG 
CAGCAGCAAA TGAATGGAGG AGAAGAGAAT GGCGAAGTGA ACGGCGTAGA CTCCTCCCCC 
ATCGCCCAGC CGGAGTACGA GACCCTGATG GCCAAGTTTC ACTTCGTGGA CCTGGCCGGC 
TCAGAAAGGC TCAAACGCAC GGGGGCCACT GGAGAAAGGG CCCGAGAGGG AATCTCTATT 
AACTGTGGAC TGCTGGCTCT TGGAAATGTA ATCAGCGCTT TAGGTGATCA GACTAAGAAA 
GCCGGACACG TCCCATATAG AGACTCCAAA CTTACCCGAC TGTTGCAAGA CTCGCTGGGA 
GGCAACAGCC ACACAGTCAT GATTGCCTGC GTCAGCCCGT CGGACCGGGA CTTCATGGAG 
ACGCTGAACA CACTGAAGTA CGCCAACCGT GCCCGAAACA TCAAGAACAA GGTGGTGGTG 
AACCAAGACA AGACCAGCCA GCAGATCAAC GCCCTACGTG CGGAGGTGGC CCGACTCCAG 
ATGGAGCTGA TGGAGTACAA GGCGGGTAAG CGTGTGGCAG GTGAGGATGG GGCTGAAGGC 
TACAGCGATC TGTACCAGGA GAACGCCATG CTGCAGAGAG AAAACGACTC GCTGCGCCTG 
AGAGTAAAGG CCATGCAGGA AACCATCGAT CATCTCAACA CACGGGTGAC ACATCTGCTG 
GCCAATGAGG TCAGCACTCT GCTCACCAAG TCAAGTGAAG GCAATGAGGA GATTGGAACG 
CTAATACAGA ACTACATCCG AGAAGTTGAA GAACTCAGAT CCAAACTCCT TGAGAGCGAG 
GCCATGAATG AGTCACTACG TCGACAGGTG GCACGGCTCT CCTCTCGTTC CTCGTTTCCA 
GCCTCCGCTC TAAGCCCAGC CCCTGGCCAT CCCCCTGGCT CTTCCCCTAT ATCCATGTCC 
ATGGAGGCCG AGATGACAGA TGTTCTGCGG CGCGCCAAGT TGGACATCGA GCGGCTGAAG 
AAGAAGGAAA GACGGCAGAG AAGGCTGAGC CCAGAGTTGG AGAAAGGTCT CAAGAAACGA 
GTAAAGCTGC ACACTCAGGA AAACGGGGAG AATCGGCGAA GCGGCGAAAA TGGACAGAAT 
GGAGAGATCG ATTCAGATGA CAATTACACC GAGGATATCA TGTCTCCGCT GCAGGAGGAG 
AGTGGCTGTG AGGAAGATGA AGGCGAAGAT GAGGAGGAGG GCAGGGAGGA AGAGGATGAT 
TTTGACAGTG ACGAGAGCCT AGTGGATTCA GATTCTGACT CGGATGAGAA AGCCAACTTC 
CAGGCAGATC TTGCCGACCT AACCTGTGAG ATTGAGATCA AGCAGAAGCT GATCGATGAG 
CTGGAGAACA GCCAGCGGCG GCTGCTGATG TTAAAGCTGC AGTACGAGGA GAAGCTCATC 
CTGCTGCAGA ACAAGATCAG AGACACGCAG CTGGAGAGAG ACCGCGTGCT GCAGAACCTC 
ATGTCCATGG AAAACTACAC TGAGGAGAAG GCCAGCAAGA TCAGGCAGGA GTACGAGAAG 
CGCCTCAAAG AAATGAACCG TGATCTGCTG AAGCTGCAGG CAGCGCAGAA GGAGCATGCA 
CGTCTGCTCA AAAACCAGGG GAGGTACGAG CGGGAACTGA AGAAGCTCCA AACTGAAGTC 
AACGAGATGA AGAAAGCAAA GGTGGTGCTG ATGAAGCAAA TGAAAGAGGA GCAACAGAGA 
AGGAGGATGG TGGAGGCAAA GAGGAATCGA GAGATTGCTC AGCTTAAGAA GGAGCAGCGG 
CGACAGGAGT ACCAGATTCG GGCGCTGGAG TCTCAAAAGA GGCAGCAGGA GCTGGTGCTG 
CGCAGGAAGA CCCAGGAAGT GACGGCCCTG CGGCGCATGG CCAAGCCCAT GTCGGACCGC 
GTGGCGGGTC GAGTTGCCCG CTGGAACCAG ACGCCTCCAG TTACGGACTC CGGAGCTGAT 
TTGTCGGCCA GCACAGCGGC AAGCAGCTCC GAGGCAGAGA CTGCGAGGAC CGTGAGCGGG 
CTGGTGCGCC AGTGGAACAA CAAGAACAAC GCGTATGGAT ACATGGCGGA GAGCGAGGGG 
AGTATGGATG GAACACGGGT GATTAGGAAG AAGCTGCAGA GGAAGCCAGT GGGCCTGGGC 
AGCACCGCAC CTGCAGGCAG AGCCAACAAC TTCTCCAAGT CTGCCCGTCT AAAGTGGCAA 
ACCCTTGAGC GTCGCATCAT GGACATCGTC ATGCAGAGAA TGACCATCTC CAACGTGGAA 
GCAGACATGG ATCGCCTTAT CAAGAAACGA GAAGAGCTGA CAATGCAGCA GGAAGCTCTC 
TCACATAAAA GGGAGATACT GATGGCCGAC GGAGAGGGGC CAGAGGCGGA GGACCGCCTC 
CTTCAGGAAG TTAACGAGGA GATCGAGGTG CTGAATGCCA ACATAGACTA CATCAATGAC 
AGTCTGTCGG ACTGCCAGGC CACCATCGTG CAGATAGAGG AGACCAAGGA TGAACTGGAC 
TCGGTGGATA CCTCAGTGGT GATCAGCTCC TGTTCCCTGG CTGAAGCACG CCACCTGCTG 
GATCACTTCC TGAAGGCTTC CATTGATAAG AGCTTACAGG TGGCTCAGAG GGAGGCGCAG 
GTACGACTGC TCGAGGGTCA GCTGAGGCAG ACTGATGTGA TCGGCTCGTC TCACAATCAC 
ATGATCCTCG ACGCGCTGCG AGAAAAAGCA GAGTGCATCC CTGAGCTCCA GGCTCTCATC 
CACAACGTCC AGCAAGAAAA TGGTTACGCC AGCACGGACG AGGAGCCCTC TGAGTTCAGC 
CAAGCCTCTG ACAGCAGTGT TTCACAGATG AAGGAATCCA ACAGCCAGGA TGACTTTAAA 
GTAAAGATGG AGCCTCGCCT CTCGGCCCAG ATGAAGGCGG TGTCTGCGGA GTACCTCGGT 
CCCATCCTGG ATCCCTCATC AAGCAGCAAG CAGCAGCACA TCACTAAGTC CCTAGCCTCA 
CTGACTGACA TCCAGGAGGA TGGCTTAAGT CTCATTCCAG GAAGTCTGGC ACTGAGCGTT 
CCCCTCCGGG ACCCTTTTCA CAGGGAACGA GCGTCCCGCA CAATCAGCCT TCCTGTCAGA 
GGACACACTT TTCCCAGGCA GTCTCGAGGT TATGACACTT CCCCCATTAC AAGAAGGAAA 
TCTTACGACC GTTCGTACAG GCCCACTGAT GTCTACACTC CCCCCTCCTC ACCTCCTTTA 
AGGACAAGGA ATGATCGCAA CGTCTTCTCC AGACTGACCA GCAACCAAGC GCAAGGTTCT 
GCTCTGGACA AGGGTGTGAT CAATCCCATT GGAGGTGTAA AGGGGGGTCG GACAGCACCC 
CTCCAGTGTG TCTCTGTTGC CGAGGGCCAC TCGAAGCCGG TTCTGTGTGT GGACGCTACA 
GATGAGCTGC TTTTCACTGG ATCTAAAGAT CGCACCTGTA AGATGTGGAA TCTAGTCACA 
GGTCAGGAGA TTGCCACCCT CAAAGGGCAC CCAAACAACG TGGTGTCGGT AAAATATTGT 
CCCTCTTCTG GTCTGGTCTT CAGCGTCTCC ACCTCATACA TCAAGGTGTG GGACATCCGT 
GACTCTGCAA AGTGCATACG CACACTCACG TCATCAGGGC AGGTAGTTAC AGGAGACGCG 
TGCGCTGGCA CAACCACCCG GACAATCACC TTTGCTCAGG GCGAGTGTCA GATCAACCAG 
ATAGCGCTCA ACCCATTAGG ATCTGTGCTC TATGCTGCTG CTGGAAATAT TGTTCGGATG 
TGGGACCTTA ACAGGATGCA GGCAACAGGC AAACTCTCAG GTCACATCGG TTCTGTCATG 
TGTCTGACAG TGGGACAGTC TCTGCTGGGC AAAGACCAGG TCATCACTGG CTCCAAAGAC 
CATTACGTCA AGATATTTGA TGTGGCAGAA GGAACTCTGG GTAATGTGGG TCCCGCTCAT 
AACTTTGAGC CTCCGCACTA TGACGGCATT GAATGTTTGG CCGTGCAGGG AGACGTGCTG 
TTCAGCGGCT CGCGAGACAA CGGTATCAAG AAGTGGGAGC TGGAGCAGCA GGAGCTCACA 
CAGCAAATCC CGAATGCCCA CAAGGACTGG GTGTGCGCTC TGGCCTATGT ACCCGGCCGG 
CCCATGTTGC TGAGCGCCTG TCGGGGTGGC ATGCTCAAGG TGTGGAATGT GGAAAACTTT 
ACGCCCATAG GAGAGGTCAG AGGTCATGAC AGCCCCATTA ATGCCATATG CACCAACTCC 
AGGCATATCT TTACAGCCTC CAGTGACTGC AGGGTGAAGT TGTGGAACTA TGTGCCAGGC 
TTGACCCCAT GTCTTCCTCG TCGGGTCCTG GCCATCAAGG GCCGAGCCAC CAGTCTCCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_023324953.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023180721.1 Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X5, mRNA.

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CloneID	OXi31292
Clone ID Related Accession (Same CDS sequence)	XM_023324953.1
Accession Version	XM_023324953.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	kinesin-like protein KIF21B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036462.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC AGAGCGATTG CTGTGTGAAG GTTGCGTTGA GGATTCGTCC TCAGATGGCC 
AAGGAGAAGA TCGAGGGCTG TCACGTGTGC ACGCTGGTCA CACCTGGAGA ACCGCAGGTC 
CTACTGGGAA AGGACAAGGC CTTCACATAC GACTTTGTGT TCGATATTGA CTCAGAGCAG 
CAGCACATCT ACCAGACCTG TGTGTACAAG CTCATCGAGG GCTGCTTCGA AGGCTACAAC 
GCAACCGTGT TTGCTTATGG ACAGACGGGC TCAGGGAAGA CCTACACCAT GGGGACGGGT 
TTTGATGTGA ATCCGAGCCT GCAGGAGCAG GGCATCATCC CACGGGCCGT TCACCACCTG 
TTTGAGGGCA TCCAGAGCAG GAGGGATCGA GCCCAGGAGA TCGGCATCCA GGCACCAGAG 
TTTAAAGTCA GCGCCCAGTT TTTGGAGTTG TACAATGAGG AGATCCTGGA CTTGTTTGAC 
GGAAGTAGAG ATCCAGAGAA TCGCAGCAGG AAGTCCAACA TCAGAATCCA CGAGGACGCC 
AACGGCAGCA TCTACACCAC AGGAGTCACT TCCAGACTGG TGCAGTCAGA GGACGAGCTG 
CTGCAGTGCC TGAAGCTTGG GGCTCTGTCC CGTACCACAG CCAGCACACA GATGAACGCA 
CAAAGCTCCC GCTCACATGC CATCTTCACC ATCCATCTCT GTCAGATGAG GGTCTTTCAG 
CAGCAGCAAA TGAATGGAGG AGAAGAGAAT GGCGAAGTGA ACGGCGTAGA CTCCTCCCCC 
ATCGCCCAGC CGGAGTACGA GACCCTGATG GCCAAGTTTC ACTTCGTGGA CCTGGCCGGC 
TCAGAAAGGC TCAAACGCAC GGGGGCCACT GGAGAAAGGG CCCGAGAGGG AATCTCTATT 
AACTGTGGAC TGCTGGCTCT TGGAAATGTA ATCAGCGCTT TAGGTGATCA GACTAAGAAA 
GCCGGACACG TCCCATATAG AGACTCCAAA CTTACCCGAC TGTTGCAAGA CTCGCTGGGA 
GGCAACAGCC ACACAGTCAT GATTGCCTGC GTCAGCCCGT CGGACCGGGA CTTCATGGAG 
ACGCTGAACA CACTGAAGTA CGCCAACCGT GCCCGAAACA TCAAGAACAA GGTGGTGGTG 
AACCAAGACA AGACCAGCCA GCAGATCAAC GCCCTACGTG CGGAGGTGGC CCGACTCCAG 
ATGGAGCTGA TGGAGTACAA GGCGGGTAAG CGTGTGGCAG GTGAGGATGG GGCTGAAGGC 
TACAGCGATC TGTACCAGGA GAACGCCATG CTGCAGAGAG AAAACGACTC GCTGCGCCTG 
AGAGTAAAGG CCATGCAGGA AACCATCGAT CATCTCAACA CACGGGTGAC ACATCTGCTG 
GCCAATGAGG TCAGCACTCT GCTCACCAAG TCAAGTGAAG GCAATGAGGA GATTGGAACG 
CTAATACAGA ACTACATCCG AGAAGTTGAA GAACTCAGAT CCAAACTCCT TGAGAGCGAG 
GCCATGAATG AGTCACTACG TCGACAGGTG GCACGGCTCT CCTCTCGTTC CTCGTTTCCA 
GCCTCCGCTC TAAGCCCAGC CCCTGGCCAT CCCCCTGGCT CTTCCCCTAT ATCCATGTCC 
ATGGAGGCCG AGATGACAGA TGTTCTGCGG CGCGCCAAGT TGGACATCGA GCGGCTGAAG 
AAGAAGGAAA GACGGCAGAG AAGGCTGAGC CCAGAGTTGG AGAAAGGTCT CAAGAAACGA 
GTAAAGCTGC ACACTCAGGA AAACGGGGAG AATCGGCGAA GCGGCGAAAA TGGACAGAAT 
GGAGAGATCG ATTCAGATGA CAATTACACC GAGGATATCA TGTCTCCGCT GCAGGAGGAG 
AGTGGCTGTG AGGAAGATGA AGGCGAAGAT GAGGAGGAGG GCAGGGAGGA AGAGGATGAT 
TTTGACAGTG ACGAGAGCCT AGTGGATTCA GATTCTGACT CGGATGAGAA AGCCAACTTC 
CAGGCAGATC TTGCCGACCT AACCTGTGAG ATTGAGATCA AGCAGAAGCT GATCGATGAG 
CTGGAGAACA GCCAGCGGCG GCTGCTGATG TTAAAGCTGC AGTACGAGGA GAAGCTCATC 
CTGCTGCAGA ACAAGATCAG AGACACGCAG CTGGAGAGAG ACCGCGTGCT GCAGAACCTC 
ATGTCCATGG AAAACTACAC TGAGGAGAAG GCCAGCAAGA TCAGGCAGGA GTACGAGAAG 
CGCCTCAAAG AAATGAACCG TGATCTGCTG AAGCTGCAGG CAGCGCAGAA GGAGCATGCA 
CGTCTGCTCA AAAACCAGGG GAGGTACGAG CGGGAACTGA AGAAGCTCCA AACTGAAGTC 
AACGAGATGA AGAAAGCAAA GGTGGTGCTG ATGAAGCAAA TGAAAGAGGA GCAACAGAGA 
AGGAGGATGG TGGAGGCAAA GAGGAATCGA GAGATTGCTC AGCTTAAGAA GGAGCAGCGG 
CGACAGGAGT ACCAGATTCG GGCGCTGGAG TCTCAAAAGA GGCAGCAGGA GCTGGTGCTG 
CGCAGGAAGA CCCAGGAAGT GACGGCCCTG CGGCGCATGG CCAAGCCCAT GTCGGACCGC 
GTGGCGGGTC GAGTTGCCCG CTGGAACCAG ACGCCTCCAG TTACGGACTC CGGAGCTGAT 
TTGTCGGCCA GCACAGCGGC AAGCAGCTCC GAGGCAGAGA CTGCGAGGAC CGTGAGCGGG 
CTGGTGCGCC AGTGGAACAA CAAGAACAAC GCGTATGGAT ACATGGCGGA GAGCGAGGGG 
AGTATGGATG GAACACGGGT GATTAGCAGG AAGAAGCTGC AGAGGAAGCC AGTGGGCCTG 
GGCAGCACCG CACCTGCAGG CAGAGCCAAC AACTTCTCCA AGTCTGCCCG TCTAAAGTGG 
CAAACCCTTG AGCGTCGCAT CATGGACATC GTCATGCAGA GAATGACCAT CTCCAACGTG 
GAAGCAGACA TGGATCGCCT TATCAAGAAA CGAGAAGAGC TGACAATGCA GCAGGAAGCT 
CTCTCACATA AAAGGGAGAT ACTGATGGCC GACGGAGAGG GGCCAGAGGC GGAGGACCGC 
CTCCTTCAGG AAGTTAACGA GGAGATCGAG GTGCTGAATG CCAACATAGA CTACATCAAT 
GACAGTCTGT CGGACTGCCA GGCCACCATC GTGCAGATAG AGGAGACCAA GGATGAACTG 
GACTCGGTGG ATACCTCAGT GGTGATCAGC TCCTGTTCCC TGGCTGAAGC ACGCCACCTG 
CTGGATCACT TCCTGAAGGC TTCCATTGAT AAGAGCTTAC AGGTGGCTCA GAGGGAGGCG 
CAGGTACGAC TGCTCGAGGG TCAGCTGAGG CAGACTGATG TGATCGGCTC GTCTCACAAT 
CACATGATCC TCGACGCGCT GCGAGAAAAA GCAGAGTGCA TCCCTGAGCT CCAGGCTCTC 
ATCCACAACG TCCAGCAAGA AAATGGTTAC GCCAGCACGG ACGAGGAGCC CTCTGAGTTC 
AGCCAAGCCT CTGACAGCAG TGTTTCACAG ATGAAGGAAT CCAACAGCCA GGATGACTTT 
AAAGTAAAGA TGGAGCCTCG CCTCTCGGCC CAGATGAAGG CGGTGTCTGC GGAGTACCTC 
GGTCCCATCC TGGATCCCTC ATCAAGCAGC AAGCAGCAGC ACATCACTAA GTCCCTAGCC 
TCACTGACTG ACATCCAGGA GGATGGCTTA AGTCTCATTC CAGGAAGTCT GGCACTGAGC 
GTTCCCCTCC GGGACCCTTT TCACAGGGAA CGAGCGTCCC GCACAATCAG CCTTCCTGTC 
AGAGGACACA CTTTTCCCAG GCAGTCTCGA GGTTATGACA CTTCCCCCAT TACAAGAAGG 
AAATCTTACG ACCGTTCGTA CAGGCCCACT GATGTCTACA CTCCCCCCTC CTCACCTCCT 
TTAAGGACAA GGAATGATCG CAACGTCTTC TCCAGACTGA CCAGCAACCA AGCGCAAGGT 
TCTGCTCTGG ACAAGTCGGA TGACAGCGAC TCCTCGCTCT CCGAGGTGCT CAGGGGTGTG 
ATCAATCCCA TTGGAGGTGT AAAGGGGGGT CGGACAGCAC CCCTCCAGTG TGTCTCTGTT 
GCCGAGGGCC ACTCGAAGCC GGTTCTGTGT GTGGACGCTA CAGATGAGCT GCTTTTCACT 
GGATCTAAAG ATCGCACCTG TAAGATGTGG AATCTAGTCA CAGGTCAGGA GATTGCCACC 
CTCAAAGGGC ACCCAAACAA CGTGGTGTCG GTAAAATATT GTCCCTCTTC TGGTCTGGTC 
TTCAGCGTCT CCACCTCATA CATCAAGGTG TGGGACATCC GTGACTCTGC AAAGTGCATA 
CGCACACTCA CGTCATCAGG GCAGGTAGTT ACAGGAGACG CGTGCGCTGG CACAACCACC 
CGGACAATCA CCTTTGCTCA GGGCGAGTGT CAGATCAACC AGATAGCGCT CAACCCATTA 
GGATCTGTGC TCTATGCTGC TGCTGGAAAT ATTGTTCGGA TGTGGGACCT TAACAGGATG 
CAGGCAACAG GCAAACTCTC AGGTCACATC GGTTCTGTCA TGTGTCTGAC AGTGGGACAG 
TCTCTGCTGG GCAAAGACCA GGTCATCACT GGCTCCAAAG ACCATTACGT CAAGATATTT 
GATGTGGCAG AAGGAACTCT GGGTAATGTG GGTCCCGCTC ATAACTTTGA GCCTCCGCAC 
TATGACGGCA TTGAATGTTT GGCCGTGCAG GGAGACGTGC TGTTCAGCGG CTCGCGAGAC 
AACGGTATCA AGAAGTGGGA GCTGGAGCAG CAGGAGCTCA CACAGCAAAT CCCGAATGCC 
CACAAGGACT GGGTGTGCGC TCTGGCCTAT GTACCCGGCC GGCCCATGTT GCTGAGCGCC 
TGTCGGGGTG GCATGCTCAA GGTGTGGAAT GTGGAAAACT TTACGCCCAT AGGAGAGGTC 
AGAGGTCATG ACAGCCCCAT TAATGCCATA TGCACCAACT CCAGGCATAT CTTTACAGCC 
TCCAGCGACA AGACAGTGAA GATCTGGCTG TCAAAGGTGT GGAGGAAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023180721.1
CDS	380..5413
Translation	MASQSDCCVKVALRIRPQMAKEKIEGCHVCTLVTPGEPQVLLGKDKAFTYDFVFDIDSEQQHIYQTCVYKLIEGCFEGYNATVFAYGQTGSGKTYTMGTGFDVNPSLQEQGIIPRAVHHLFEGIQSRRDRAQEIGIQAPEFKVSAQFLELYNEEILDLFDGSRDPENRSRKSNIRIHEDANGSIYTTGVTSRLVQSEDELLQCLKLGALSRTTASTQMNAQSSRSHAIFTIHLCQMRVFQQQQMNGGEENGEVNGVDSSPIAQPEYETLMAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQTKKAGHVPYRDSKLTRLLQDSLGGNSHTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQINALRAEVARLQMELMEYKAGKRVAGEDGAEGYSDLYQENAMLQRENDSLRLRVKAMQETIDHLNTRVTHLLANEVSTLLTKSSEGNEEIGTLIQNYIREVEELRSKLLESEAMNESLRRQVARLSSRSSFPASALSPAPGHPPGSSPISMSMEAEMTDVLRRAKLDIERLKKKERRQRRLSPELEKGLKKRVKLHTQENGENRRSGENGQNGEIDSDDNYTEDIMSPLQEESGCEEDEGEDEEEGREEEDDFDSDESLVDSDSDSDEKANFQADLADLTCEIEIKQKLIDELENSQRRLLMLKLQYEEKLILLQNKIRDTQLERDRVLQNLMSMENYTEEKASKIRQEYEKRLKEMNRDLLKLQAAQKEHARLLKNQGRYERELKKLQTEVNEMKKAKVVLMKQMKEEQQRRRMVEAKRNREIAQLKKEQRRQEYQIRALESQKRQQELVLRRKTQEVTALRRMAKPMSDRVAGRVARWNQTPPVTDSGADLSASTAASSSEAETARTVSGLVRQWNNKNNAYGYMAESEGSMDGTRVISRKKLQRKPVGLGSTAPAGRANNFSKSARLKWQTLERRIMDIVMQRMTISNVEADMDRLIKKREELTMQQEALSHKREILMADGEGPEAEDRLLQEVNEEIEVLNANIDYINDSLSDCQATIVQIEETKDELDSVDTSVVISSCSLAEARHLLDHFLKASIDKSLQVAQREAQVRLLEGQLRQTDVIGSSHNHMILDALREKAECIPELQALIHNVQQENGYASTDEEPSEFSQASDSSVSQMKESNSQDDFKVKMEPRLSAQMKAVSAEYLGPILDPSSSSKQQHITKSLASLTDIQEDGLSLIPGSLALSVPLRDPFHRERASRTISLPVRGHTFPRQSRGYDTSPITRRKSYDRSYRPTDVYTPPSSPPLRTRNDRNVFSRLTSNQAQGSALDKSDDSDSSLSEVLRGVINPIGGVKGGRTAPLQCVSVAEGHSKPVLCVDATDELLFTGSKDRTCKMWNLVTGQEIATLKGHPNNVVSVKYCPSSGLVFSVSTSYIKVWDIRDSAKCIRTLTSSGQVVTGDACAGTTTRTITFAQGECQINQIALNPLGSVLYAAAGNIVRMWDLNRMQATGKLSGHIGSVMCLTVGQSLLGKDQVITGSKDHYVKIFDVAEGTLGNVGPAHNFEPPHYDGIECLAVQGDVLFSGSRDNGIKKWELEQQELTQQIPNAHKDWVCALAYVPGRPMLLSACRGGMLKVWNVENFTPIGEVRGHDSPINAICTNSRHIFTASSDKTVKIWLSKVWRKR

Target ORF information:

RefSeq Version	XM_023324953.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023324953.1

ORF Insert Sequence:

ATGGCGAGCC AGAGCGATTG CTGTGTGAAG GTTGCGTTGA GGATTCGTCC TCAGATGGCC 
AAGGAGAAGA TCGAGGGCTG TCACGTGTGC ACGCTGGTCA CACCTGGAGA ACCGCAGGTC 
CTACTGGGAA AGGACAAGGC CTTCACATAC GACTTTGTGT TCGATATTGA CTCAGAGCAG 
CAGCACATCT ACCAGACCTG TGTGTACAAG CTCATCGAGG GCTGCTTCGA AGGCTACAAC 
GCAACCGTGT TTGCTTATGG ACAGACGGGC TCAGGGAAGA CCTACACCAT GGGGACGGGT 
TTTGATGTGA ATCCGAGCCT GCAGGAGCAG GGCATCATCC CACGGGCCGT TCACCACCTG 
TTTGAGGGCA TCCAGAGCAG GAGGGATCGA GCCCAGGAGA TCGGCATCCA GGCACCAGAG 
TTTAAAGTCA GCGCCCAGTT TTTGGAGTTG TACAATGAGG AGATCCTGGA CTTGTTTGAC 
GGAAGTAGAG ATCCAGAGAA TCGCAGCAGG AAGTCCAACA TCAGAATCCA CGAGGACGCC 
AACGGCAGCA TCTACACCAC AGGAGTCACT TCCAGACTGG TGCAGTCAGA GGACGAGCTG 
CTGCAGTGCC TGAAGCTTGG GGCTCTGTCC CGTACCACAG CCAGCACACA GATGAACGCA 
CAAAGCTCCC GCTCACATGC CATCTTCACC ATCCATCTCT GTCAGATGAG GGTCTTTCAG 
CAGCAGCAAA TGAATGGAGG AGAAGAGAAT GGCGAAGTGA ACGGCGTAGA CTCCTCCCCC 
ATCGCCCAGC CGGAGTACGA GACCCTGATG GCCAAGTTTC ACTTCGTGGA CCTGGCCGGC 
TCAGAAAGGC TCAAACGCAC GGGGGCCACT GGAGAAAGGG CCCGAGAGGG AATCTCTATT 
AACTGTGGAC TGCTGGCTCT TGGAAATGTA ATCAGCGCTT TAGGTGATCA GACTAAGAAA 
GCCGGACACG TCCCATATAG AGACTCCAAA CTTACCCGAC TGTTGCAAGA CTCGCTGGGA 
GGCAACAGCC ACACAGTCAT GATTGCCTGC GTCAGCCCGT CGGACCGGGA CTTCATGGAG 
ACGCTGAACA CACTGAAGTA CGCCAACCGT GCCCGAAACA TCAAGAACAA GGTGGTGGTG 
AACCAAGACA AGACCAGCCA GCAGATCAAC GCCCTACGTG CGGAGGTGGC CCGACTCCAG 
ATGGAGCTGA TGGAGTACAA GGCGGGTAAG CGTGTGGCAG GTGAGGATGG GGCTGAAGGC 
TACAGCGATC TGTACCAGGA GAACGCCATG CTGCAGAGAG AAAACGACTC GCTGCGCCTG 
AGAGTAAAGG CCATGCAGGA AACCATCGAT CATCTCAACA CACGGGTGAC ACATCTGCTG 
GCCAATGAGG TCAGCACTCT GCTCACCAAG TCAAGTGAAG GCAATGAGGA GATTGGAACG 
CTAATACAGA ACTACATCCG AGAAGTTGAA GAACTCAGAT CCAAACTCCT TGAGAGCGAG 
GCCATGAATG AGTCACTACG TCGACAGGTG GCACGGCTCT CCTCTCGTTC CTCGTTTCCA 
GCCTCCGCTC TAAGCCCAGC CCCTGGCCAT CCCCCTGGCT CTTCCCCTAT ATCCATGTCC 
ATGGAGGCCG AGATGACAGA TGTTCTGCGG CGCGCCAAGT TGGACATCGA GCGGCTGAAG 
AAGAAGGAAA GACGGCAGAG AAGGCTGAGC CCAGAGTTGG AGAAAGGTCT CAAGAAACGA 
GTAAAGCTGC ACACTCAGGA AAACGGGGAG AATCGGCGAA GCGGCGAAAA TGGACAGAAT 
GGAGAGATCG ATTCAGATGA CAATTACACC GAGGATATCA TGTCTCCGCT GCAGGAGGAG 
AGTGGCTGTG AGGAAGATGA AGGCGAAGAT GAGGAGGAGG GCAGGGAGGA AGAGGATGAT 
TTTGACAGTG ACGAGAGCCT AGTGGATTCA GATTCTGACT CGGATGAGAA AGCCAACTTC 
CAGGCAGATC TTGCCGACCT AACCTGTGAG ATTGAGATCA AGCAGAAGCT GATCGATGAG 
CTGGAGAACA GCCAGCGGCG GCTGCTGATG TTAAAGCTGC AGTACGAGGA GAAGCTCATC 
CTGCTGCAGA ACAAGATCAG AGACACGCAG CTGGAGAGAG ACCGCGTGCT GCAGAACCTC 
ATGTCCATGG AAAACTACAC TGAGGAGAAG GCCAGCAAGA TCAGGCAGGA GTACGAGAAG 
CGCCTCAAAG AAATGAACCG TGATCTGCTG AAGCTGCAGG CAGCGCAGAA GGAGCATGCA 
CGTCTGCTCA AAAACCAGGG GAGGTACGAG CGGGAACTGA AGAAGCTCCA AACTGAAGTC 
AACGAGATGA AGAAAGCAAA GGTGGTGCTG ATGAAGCAAA TGAAAGAGGA GCAACAGAGA 
AGGAGGATGG TGGAGGCAAA GAGGAATCGA GAGATTGCTC AGCTTAAGAA GGAGCAGCGG 
CGACAGGAGT ACCAGATTCG GGCGCTGGAG TCTCAAAAGA GGCAGCAGGA GCTGGTGCTG 
CGCAGGAAGA CCCAGGAAGT GACGGCCCTG CGGCGCATGG CCAAGCCCAT GTCGGACCGC 
GTGGCGGGTC GAGTTGCCCG CTGGAACCAG ACGCCTCCAG TTACGGACTC CGGAGCTGAT 
TTGTCGGCCA GCACAGCGGC AAGCAGCTCC GAGGCAGAGA CTGCGAGGAC CGTGAGCGGG 
CTGGTGCGCC AGTGGAACAA CAAGAACAAC GCGTATGGAT ACATGGCGGA GAGCGAGGGG 
AGTATGGATG GAACACGGGT GATTAGCAGG AAGAAGCTGC AGAGGAAGCC AGTGGGCCTG 
GGCAGCACCG CACCTGCAGG CAGAGCCAAC AACTTCTCCA AGTCTGCCCG TCTAAAGTGG 
CAAACCCTTG AGCGTCGCAT CATGGACATC GTCATGCAGA GAATGACCAT CTCCAACGTG 
GAAGCAGACA TGGATCGCCT TATCAAGAAA CGAGAAGAGC TGACAATGCA GCAGGAAGCT 
CTCTCACATA AAAGGGAGAT ACTGATGGCC GACGGAGAGG GGCCAGAGGC GGAGGACCGC 
CTCCTTCAGG AAGTTAACGA GGAGATCGAG GTGCTGAATG CCAACATAGA CTACATCAAT 
GACAGTCTGT CGGACTGCCA GGCCACCATC GTGCAGATAG AGGAGACCAA GGATGAACTG 
GACTCGGTGG ATACCTCAGT GGTGATCAGC TCCTGTTCCC TGGCTGAAGC ACGCCACCTG 
CTGGATCACT TCCTGAAGGC TTCCATTGAT AAGAGCTTAC AGGTGGCTCA GAGGGAGGCG 
CAGGTACGAC TGCTCGAGGG TCAGCTGAGG CAGACTGATG TGATCGGCTC GTCTCACAAT 
CACATGATCC TCGACGCGCT GCGAGAAAAA GCAGAGTGCA TCCCTGAGCT CCAGGCTCTC 
ATCCACAACG TCCAGCAAGA AAATGGTTAC GCCAGCACGG ACGAGGAGCC CTCTGAGTTC 
AGCCAAGCCT CTGACAGCAG TGTTTCACAG ATGAAGGAAT CCAACAGCCA GGATGACTTT 
AAAGTAAAGA TGGAGCCTCG CCTCTCGGCC CAGATGAAGG CGGTGTCTGC GGAGTACCTC 
GGTCCCATCC TGGATCCCTC ATCAAGCAGC AAGCAGCAGC ACATCACTAA GTCCCTAGCC 
TCACTGACTG ACATCCAGGA GGATGGCTTA AGTCTCATTC CAGGAAGTCT GGCACTGAGC 
GTTCCCCTCC GGGACCCTTT TCACAGGGAA CGAGCGTCCC GCACAATCAG CCTTCCTGTC 
AGAGGACACA CTTTTCCCAG GCAGTCTCGA GGTTATGACA CTTCCCCCAT TACAAGAAGG 
AAATCTTACG ACCGTTCGTA CAGGCCCACT GATGTCTACA CTCCCCCCTC CTCACCTCCT 
TTAAGGACAA GGAATGATCG CAACGTCTTC TCCAGACTGA CCAGCAACCA AGCGCAAGGT 
TCTGCTCTGG ACAAGTCGGA TGACAGCGAC TCCTCGCTCT CCGAGGTGCT CAGGGGTGTG 
ATCAATCCCA TTGGAGGTGT AAAGGGGGGT CGGACAGCAC CCCTCCAGTG TGTCTCTGTT 
GCCGAGGGCC ACTCGAAGCC GGTTCTGTGT GTGGACGCTA CAGATGAGCT GCTTTTCACT 
GGATCTAAAG ATCGCACCTG TAAGATGTGG AATCTAGTCA CAGGTCAGGA GATTGCCACC 
CTCAAAGGGC ACCCAAACAA CGTGGTGTCG GTAAAATATT GTCCCTCTTC TGGTCTGGTC 
TTCAGCGTCT CCACCTCATA CATCAAGGTG TGGGACATCC GTGACTCTGC AAAGTGCATA 
CGCACACTCA CGTCATCAGG GCAGGTAGTT ACAGGAGACG CGTGCGCTGG CACAACCACC 
CGGACAATCA CCTTTGCTCA GGGCGAGTGT CAGATCAACC AGATAGCGCT CAACCCATTA 
GGATCTGTGC TCTATGCTGC TGCTGGAAAT ATTGTTCGGA TGTGGGACCT TAACAGGATG 
CAGGCAACAG GCAAACTCTC AGGTCACATC GGTTCTGTCA TGTGTCTGAC AGTGGGACAG 
TCTCTGCTGG GCAAAGACCA GGTCATCACT GGCTCCAAAG ACCATTACGT CAAGATATTT 
GATGTGGCAG AAGGAACTCT GGGTAATGTG GGTCCCGCTC ATAACTTTGA GCCTCCGCAC 
TATGACGGCA TTGAATGTTT GGCCGTGCAG GGAGACGTGC TGTTCAGCGG CTCGCGAGAC 
AACGGTATCA AGAAGTGGGA GCTGGAGCAG CAGGAGCTCA CACAGCAAAT CCCGAATGCC 
CACAAGGACT GGGTGTGCGC TCTGGCCTAT GTACCCGGCC GGCCCATGTT GCTGAGCGCC 
TGTCGGGGTG GCATGCTCAA GGTGTGGAAT GTGGAAAACT TTACGCCCAT AGGAGAGGTC 
AGAGGTCATG ACAGCCCCAT TAATGCCATA TGCACCAACT CCAGGCATAT CTTTACAGCC 
TCCAGCGACA AGACAGTGAA GATCTGGCTG TCAAAGGTGT GGAGGAAGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_023324955.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023180723.1 Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X6, mRNA.

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CloneID	OXi31293
Clone ID Related Accession (Same CDS sequence)	XM_023324955.1
Accession Version	XM_023324955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	kinesin-like protein KIF21B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036462.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCC AGAGCGATTG CTGTGTGAAG GTTGCGTTGA GGATTCGTCC TCAGATGGCC 
AAGGAGAAGA TCGAGGGCTG TCACGTGTGC ACGCTGGTCA CACCTGGAGA ACCGCAGGTC 
CTACTGGGAA AGGACAAGGC CTTCACATAC GACTTTGTGT TCGATATTGA CTCAGAGCAG 
CAGCACATCT ACCAGACCTG TGTGTACAAG CTCATCGAGG GCTGCTTCGA AGGCTACAAC 
GCAACCGTGT TTGCTTATGG ACAGACGGGC TCAGGGAAGA CCTACACCAT GGGGACGGGT 
TTTGATGTGA ATCCGAGCCT GCAGGAGCAG GGCATCATCC CACGGGCCGT TCACCACCTG 
TTTGAGGGCA TCCAGAGCAG GAGGGATCGA GCCCAGGAGA TCGGCATCCA GGCACCAGAG 
TTTAAAGTCA GCGCCCAGTT TTTGGAGTTG TACAATGAGG AGATCCTGGA CTTGTTTGAC 
GGAAGTAGAG ATCCAGAGAA TCGCAGCAGG AAGTCCAACA TCAGAATCCA CGAGGACGCC 
AACGGCAGCA TCTACACCAC AGGAGTCACT TCCAGACTGG TGCAGTCAGA GGACGAGCTG 
CTGCAGTGCC TGAAGCTTGG GGCTCTGTCC CGTACCACAG CCAGCACACA GATGAACGCA 
CAAAGCTCCC GCTCACATGC CATCTTCACC ATCCATCTCT GTCAGATGAG GGTCTTTCAG 
CAGCAGCAAA TGAATGGAGG AGAAGAGAAT GGCGAAGTGA ACGGCGTAGA CTCCTCCCCC 
ATCGCCCAGC CGGAGTACGA GACCCTGATG GCCAAGTTTC ACTTCGTGGA CCTGGCCGGC 
TCAGAAAGGC TCAAACGCAC GGGGGCCACT GGAGAAAGGG CCCGAGAGGG AATCTCTATT 
AACTGTGGAC TGCTGGCTCT TGGAAATGTA ATCAGCGCTT TAGGTGATCA GACTAAGAAA 
GCCGGACACG TCCCATATAG AGACTCCAAA CTTACCCGAC TGTTGCAAGA CTCGCTGGGA 
GGCAACAGCC ACACAGTCAT GATTGCCTGC GTCAGCCCGT CGGACCGGGA CTTCATGGAG 
ACGCTGAACA CACTGAAGTA CGCCAACCGT GCCCGAAACA TCAAGAACAA GGTGGTGGTG 
AACCAAGACA AGACCAGCCA GCAGATCAAC GCCCTACGTG CGGAGGTGGC CCGACTCCAG 
ATGGAGCTGA TGGAGTACAA GGCGGGTAAG CGTGTGGCAG GTGAGGATGG GGCTGAAGGC 
TACAGCGATC TGTACCAGGA GAACGCCATG CTGCAGAGAG AAAACGACTC GCTGCGCCTG 
AGAGTAAAGG CCATGCAGGA AACCATCGAT CATCTCAACA CACGGGTGAC ACATCTGCTG 
GCCAATGAGG TCAGCACTCT GCTCACCAAG TCAAGTGAAG GCAATGAGGA GATTGGAACG 
CTAATACAGA ACTACATCCG AGAAGTTGAA GAACTCAGAT CCAAACTCCT TGAGAGCGAG 
GCCATGAATG AGTCACTACG TCGACAGGTG GCACGGCTCT CCTCTCGTTC CTCGTTTCCA 
GCCTCCGCTC TAAGCCCAGC CCCTGGCCAT CCCCCTGGCT CTTCCCCTAT ATCCATGTCC 
ATGGAGGCCG AGATGACAGA TGTTCTGCGG CGCGCCAAGT TGGACATCGA GCGGCTGAAG 
AAGAAGGAAA GACGGCAGAG AAGGCTGAGC CCAGAGTTGG AGAAAGGTCT CAAGAAACGA 
GTAAAGCTGC ACACTCAGGA AAACGGGGAG AATCGGCGAA GCGGCGAAAA TGGACAGAAT 
GGAGAGATCG ATTCAGATGA CAATTACACC GAGGATATCA TGTCTCCGCT GCAGGAGGAG 
AGTGGCTGTG AGGAAGATGA AGGCGAAGAT GAGGAGGAGG GCAGGGAGGA AGAGGATGAT 
TTTGACAGTG ACGAGAGCCT AGTGGATTCA GATTCTGACT CGGATGAGAA AGCCAACTTC 
CAGGCAGATC TTGCCGACCT AACCTGTGAG ATTGAGATCA AGCAGAAGCT GATCGATGAG 
CTGGAGAACA GCCAGCGGCG GCTGCTGATG TTAAAGCTGC AGTACGAGGA GAAGCTCATC 
CTGCTGCAGA ACAAGATCAG AGACACGCAG CTGGAGAGAG ACCGCGTGCT GCAGAACCTC 
ATGTCCATGG AAAACTACAC TGAGGAGAAG GCCAGCAAGA TCAGGCAGGA GTACGAGAAG 
CGCCTCAAAG AAATGAACCG TGATCTGCTG AAGCTGCAGG CAGCGCAGAA GGAGCATGCA 
CGTCTGCTCA AAAACCAGGG GAGGTACGAG CGGGAACTGA AGAAGCTCCA AACTGAAGTC 
AACGAGATGA AGAAAGCAAA GGTGGTGCTG ATGAAGCAAA TGAAAGAGGA GCAACAGAGA 
AGGAGGATGG TGGAGGCAAA GAGGAATCGA GAGATTGCTC AGCTTAAGAA GGAGCAGCGG 
CGACAGGAGT ACCAGATTCG GGCGCTGGAG TCTCAAAAGA GGCAGCAGGA GCTGGTGCTG 
CGCAGGAAGA CCCAGGAAGT GACGGCCCTG CGGCGCATGG CCAAGCCCAT GTCGGACCGC 
GTGGCGGGTC GAGTTGCCCG CTGGAACCAG ACGCCTCCAG TTACGGACTC CGGAGCTGAT 
TTGTCGGCCA GCACAGCGGC AAGCAGCTCC GAGGCAGAGA CTGCGAGGAC CGTGAGCGGG 
CTGGTGCGCC AGTGGAACAA CAAGAACAAC GCGTATGGAT ACATGGCGGA GAGCGAGGGG 
AGTATGGATG GAACACGGGT GATTAGCAGG AAGAAGCTGC AGAGGAAGCC AGTGGGCCTG 
GGCAGCACCG CACCTGCAGG CAGAGCCAAC AACTTCTCCA AGTCTGCCCG TCTAAAGTGG 
CAAACCCTTG AGCGTCGCAT CATGGACATC GTCATGCAGA GAATGACCAT CTCCAACGTG 
GAAGCAGACA TGGATCGCCT TATCAAGAAA CGAGAAGAGC TGACAATGCA GCAGGAAGCT 
CTCTCACATA AAAGGGAGAT ACTGATGGCC GACGGAGAGG GGCCAGAGGC GGAGGACCGC 
CTCCTTCAGG AAGTTAACGA GGAGATCGAG GTGCTGAATG CCAACATAGA CTACATCAAT 
GACAGTCTGT CGGACTGCCA GGCCACCATC GTGCAGATAG AGGAGACCAA GGATGAACTG 
GACTCGGTGG ATACCTCAGT GGTGATCAGC TCCTGTTCCC TGGCTGAAGC ACGCCACCTG 
CTGGATCACT TCCTGAAGGC TTCCATTGAT AAGAGCTTAC AGGTGGCTCA GAGGGAGGCG 
CAGGTACGAC TGCTCGAGGG TCAGCTGAGG CAGACTGATG TGATCGGCTC GTCTCACAAT 
CACATGATCC TCGACGCGCT GCGAGAAAAA GCAGAGTGCA TCCCTGAGCT CCAGGCTCTC 
ATCCACAACG TCCAGCAAGA AAATGGTTAC GCCAGCACGG ACGAGGAGCC CTCTGAGTTC 
AGCCAAGCCT CTGACAGCAG TGTTTCACAG ATGAAGGAAT CCAACAGCCA GGATGACTTT 
AAAGTAAAGA TGGAGCCTCG CCTCTCGGCC CAGATGAAGG CGGTGTCTGC GGAGTACCTC 
GGTCCCATCC TGGATCCCTC ATCAAGCAGC AAGCAGCAGC ACATCACTAA GTCCCTAGCC 
TCACTGACTG ACATCCAGGA GGATGGCTTA AGTCTCATTC CAGGAAGTCT GGCACTGAGC 
GTTCCCCTCC GGGACCCTTT TCACAGGGAA CGAGCGTCCC GCACAATCAG CCTTCCTGTC 
AGAGGACACA CTTTTCCCAG GCAGTCTCGA GGTTATGACA CTTCCCCCAT TACAAGAAGG 
AAATCTTACG ACCGTTCGTA CAGGCCCACT GATGTCTACA CTCCCCCCTC CTCACCTCCT 
TTAAGGACAA GGAATGATCG CAACGTCTTC TCCAGACTGA CCAGCAACCA AGCGCAAGGT 
TCTGCTCTGG ACAAGGGTGT GATCAATCCC ATTGGAGGTG TAAAGGGGGG TCGGACAGCA 
CCCCTCCAGT GTGTCTCTGT TGCCGAGGGC CACTCGAAGC CGGTTCTGTG TGTGGACGCT 
ACAGATGAGC TGCTTTTCAC TGGATCTAAA GATCGCACCT GTAAGATGTG GAATCTAGTC 
ACAGGTCAGG AGATTGCCAC CCTCAAAGGG CACCCAAACA ACGTGGTGTC GGTAAAATAT 
TGTCCCTCTT CTGGTCTGGT CTTCAGCGTC TCCACCTCAT ACATCAAGGT GTGGGACATC 
CGTGACTCTG CAAAGTGCAT ACGCACACTC ACGTCATCAG GGCAGGTAGT TACAGGAGAC 
GCGTGCGCTG GCACAACCAC CCGGACAATC ACCTTTGCTC AGGGCGAGTG TCAGATCAAC 
CAGATAGCGC TCAACCCATT AGGATCTGTG CTCTATGCTG CTGCTGGAAA TATTGTTCGG 
ATGTGGGACC TTAACAGGAT GCAGGCAACA GGCAAACTCT CAGGTCACAT CGGTTCTGTC 
ATGTGTCTGA CAGTGGGACA GTCTCTGCTG GGCAAAGACC AGGTCATCAC TGGCTCCAAA 
GACCATTACG TCAAGATATT TGATGTGGCA GAAGGAACTC TGGGTAATGT GGGTCCCGCT 
CATAACTTTG AGCCTCCGCA CTATGACGGC ATTGAATGTT TGGCCGTGCA GGGAGACGTG 
CTGTTCAGCG GCTCGCGAGA CAACGGTATC AAGAAGTGGG AGCTGGAGCA GCAGGAGCTC 
ACACAGCAAA TCCCGAATGC CCACAAGGAC TGGGTGTGCG CTCTGGCCTA TGTACCCGGC 
CGGCCCATGT TGCTGAGCGC CTGTCGGGGT GGCATGCTCA AGGTGTGGAA TGTGGAAAAC 
TTTACGCCCA TAGGAGAGGT CAGAGGTCAT GACAGCCCCA TTAATGCCAT ATGCACCAAC 
TCCAGGCATA TCTTTACAGC CTCCAGCGAC AAGACAGTGA AGATCTGGCT GTCAAAGGTG 
TGGAGGAAGA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023180723.1
CDS	380..5374
Translation	MASQSDCCVKVALRIRPQMAKEKIEGCHVCTLVTPGEPQVLLGKDKAFTYDFVFDIDSEQQHIYQTCVYKLIEGCFEGYNATVFAYGQTGSGKTYTMGTGFDVNPSLQEQGIIPRAVHHLFEGIQSRRDRAQEIGIQAPEFKVSAQFLELYNEEILDLFDGSRDPENRSRKSNIRIHEDANGSIYTTGVTSRLVQSEDELLQCLKLGALSRTTASTQMNAQSSRSHAIFTIHLCQMRVFQQQQMNGGEENGEVNGVDSSPIAQPEYETLMAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQTKKAGHVPYRDSKLTRLLQDSLGGNSHTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQINALRAEVARLQMELMEYKAGKRVAGEDGAEGYSDLYQENAMLQRENDSLRLRVKAMQETIDHLNTRVTHLLANEVSTLLTKSSEGNEEIGTLIQNYIREVEELRSKLLESEAMNESLRRQVARLSSRSSFPASALSPAPGHPPGSSPISMSMEAEMTDVLRRAKLDIERLKKKERRQRRLSPELEKGLKKRVKLHTQENGENRRSGENGQNGEIDSDDNYTEDIMSPLQEESGCEEDEGEDEEEGREEEDDFDSDESLVDSDSDSDEKANFQADLADLTCEIEIKQKLIDELENSQRRLLMLKLQYEEKLILLQNKIRDTQLERDRVLQNLMSMENYTEEKASKIRQEYEKRLKEMNRDLLKLQAAQKEHARLLKNQGRYERELKKLQTEVNEMKKAKVVLMKQMKEEQQRRRMVEAKRNREIAQLKKEQRRQEYQIRALESQKRQQELVLRRKTQEVTALRRMAKPMSDRVAGRVARWNQTPPVTDSGADLSASTAASSSEAETARTVSGLVRQWNNKNNAYGYMAESEGSMDGTRVISRKKLQRKPVGLGSTAPAGRANNFSKSARLKWQTLERRIMDIVMQRMTISNVEADMDRLIKKREELTMQQEALSHKREILMADGEGPEAEDRLLQEVNEEIEVLNANIDYINDSLSDCQATIVQIEETKDELDSVDTSVVISSCSLAEARHLLDHFLKASIDKSLQVAQREAQVRLLEGQLRQTDVIGSSHNHMILDALREKAECIPELQALIHNVQQENGYASTDEEPSEFSQASDSSVSQMKESNSQDDFKVKMEPRLSAQMKAVSAEYLGPILDPSSSSKQQHITKSLASLTDIQEDGLSLIPGSLALSVPLRDPFHRERASRTISLPVRGHTFPRQSRGYDTSPITRRKSYDRSYRPTDVYTPPSSPPLRTRNDRNVFSRLTSNQAQGSALDKGVINPIGGVKGGRTAPLQCVSVAEGHSKPVLCVDATDELLFTGSKDRTCKMWNLVTGQEIATLKGHPNNVVSVKYCPSSGLVFSVSTSYIKVWDIRDSAKCIRTLTSSGQVVTGDACAGTTTRTITFAQGECQINQIALNPLGSVLYAAAGNIVRMWDLNRMQATGKLSGHIGSVMCLTVGQSLLGKDQVITGSKDHYVKIFDVAEGTLGNVGPAHNFEPPHYDGIECLAVQGDVLFSGSRDNGIKKWELEQQELTQQIPNAHKDWVCALAYVPGRPMLLSACRGGMLKVWNVENFTPIGEVRGHDSPINAICTNSRHIFTASSDKTVKIWLSKVWRKR

Target ORF information:

RefSeq Version	XM_023324955.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus kinesin family member 21B (kif21b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023324955.1

ORF Insert Sequence:

ATGGCGAGCC AGAGCGATTG CTGTGTGAAG GTTGCGTTGA GGATTCGTCC TCAGATGGCC 
AAGGAGAAGA TCGAGGGCTG TCACGTGTGC ACGCTGGTCA CACCTGGAGA ACCGCAGGTC 
CTACTGGGAA AGGACAAGGC CTTCACATAC GACTTTGTGT TCGATATTGA CTCAGAGCAG 
CAGCACATCT ACCAGACCTG TGTGTACAAG CTCATCGAGG GCTGCTTCGA AGGCTACAAC 
GCAACCGTGT TTGCTTATGG ACAGACGGGC TCAGGGAAGA CCTACACCAT GGGGACGGGT 
TTTGATGTGA ATCCGAGCCT GCAGGAGCAG GGCATCATCC CACGGGCCGT TCACCACCTG 
TTTGAGGGCA TCCAGAGCAG GAGGGATCGA GCCCAGGAGA TCGGCATCCA GGCACCAGAG 
TTTAAAGTCA GCGCCCAGTT TTTGGAGTTG TACAATGAGG AGATCCTGGA CTTGTTTGAC 
GGAAGTAGAG ATCCAGAGAA TCGCAGCAGG AAGTCCAACA TCAGAATCCA CGAGGACGCC 
AACGGCAGCA TCTACACCAC AGGAGTCACT TCCAGACTGG TGCAGTCAGA GGACGAGCTG 
CTGCAGTGCC TGAAGCTTGG GGCTCTGTCC CGTACCACAG CCAGCACACA GATGAACGCA 
CAAAGCTCCC GCTCACATGC CATCTTCACC ATCCATCTCT GTCAGATGAG GGTCTTTCAG 
CAGCAGCAAA TGAATGGAGG AGAAGAGAAT GGCGAAGTGA ACGGCGTAGA CTCCTCCCCC 
ATCGCCCAGC CGGAGTACGA GACCCTGATG GCCAAGTTTC ACTTCGTGGA CCTGGCCGGC 
TCAGAAAGGC TCAAACGCAC GGGGGCCACT GGAGAAAGGG CCCGAGAGGG AATCTCTATT 
AACTGTGGAC TGCTGGCTCT TGGAAATGTA ATCAGCGCTT TAGGTGATCA GACTAAGAAA 
GCCGGACACG TCCCATATAG AGACTCCAAA CTTACCCGAC TGTTGCAAGA CTCGCTGGGA 
GGCAACAGCC ACACAGTCAT GATTGCCTGC GTCAGCCCGT CGGACCGGGA CTTCATGGAG 
ACGCTGAACA CACTGAAGTA CGCCAACCGT GCCCGAAACA TCAAGAACAA GGTGGTGGTG 
AACCAAGACA AGACCAGCCA GCAGATCAAC GCCCTACGTG CGGAGGTGGC CCGACTCCAG 
ATGGAGCTGA TGGAGTACAA GGCGGGTAAG CGTGTGGCAG GTGAGGATGG GGCTGAAGGC 
TACAGCGATC TGTACCAGGA GAACGCCATG CTGCAGAGAG AAAACGACTC GCTGCGCCTG 
AGAGTAAAGG CCATGCAGGA AACCATCGAT CATCTCAACA CACGGGTGAC ACATCTGCTG 
GCCAATGAGG TCAGCACTCT GCTCACCAAG TCAAGTGAAG GCAATGAGGA GATTGGAACG 
CTAATACAGA ACTACATCCG AGAAGTTGAA GAACTCAGAT CCAAACTCCT TGAGAGCGAG 
GCCATGAATG AGTCACTACG TCGACAGGTG GCACGGCTCT CCTCTCGTTC CTCGTTTCCA 
GCCTCCGCTC TAAGCCCAGC CCCTGGCCAT CCCCCTGGCT CTTCCCCTAT ATCCATGTCC 
ATGGAGGCCG AGATGACAGA TGTTCTGCGG CGCGCCAAGT TGGACATCGA GCGGCTGAAG 
AAGAAGGAAA GACGGCAGAG AAGGCTGAGC CCAGAGTTGG AGAAAGGTCT CAAGAAACGA 
GTAAAGCTGC ACACTCAGGA AAACGGGGAG AATCGGCGAA GCGGCGAAAA TGGACAGAAT 
GGAGAGATCG ATTCAGATGA CAATTACACC GAGGATATCA TGTCTCCGCT GCAGGAGGAG 
AGTGGCTGTG AGGAAGATGA AGGCGAAGAT GAGGAGGAGG GCAGGGAGGA AGAGGATGAT 
TTTGACAGTG ACGAGAGCCT AGTGGATTCA GATTCTGACT CGGATGAGAA AGCCAACTTC 
CAGGCAGATC TTGCCGACCT AACCTGTGAG ATTGAGATCA AGCAGAAGCT GATCGATGAG 
CTGGAGAACA GCCAGCGGCG GCTGCTGATG TTAAAGCTGC AGTACGAGGA GAAGCTCATC 
CTGCTGCAGA ACAAGATCAG AGACACGCAG CTGGAGAGAG ACCGCGTGCT GCAGAACCTC 
ATGTCCATGG AAAACTACAC TGAGGAGAAG GCCAGCAAGA TCAGGCAGGA GTACGAGAAG 
CGCCTCAAAG AAATGAACCG TGATCTGCTG AAGCTGCAGG CAGCGCAGAA GGAGCATGCA 
CGTCTGCTCA AAAACCAGGG GAGGTACGAG CGGGAACTGA AGAAGCTCCA AACTGAAGTC 
AACGAGATGA AGAAAGCAAA GGTGGTGCTG ATGAAGCAAA TGAAAGAGGA GCAACAGAGA 
AGGAGGATGG TGGAGGCAAA GAGGAATCGA GAGATTGCTC AGCTTAAGAA GGAGCAGCGG 
CGACAGGAGT ACCAGATTCG GGCGCTGGAG TCTCAAAAGA GGCAGCAGGA GCTGGTGCTG 
CGCAGGAAGA CCCAGGAAGT GACGGCCCTG CGGCGCATGG CCAAGCCCAT GTCGGACCGC 
GTGGCGGGTC GAGTTGCCCG CTGGAACCAG ACGCCTCCAG TTACGGACTC CGGAGCTGAT 
TTGTCGGCCA GCACAGCGGC AAGCAGCTCC GAGGCAGAGA CTGCGAGGAC CGTGAGCGGG 
CTGGTGCGCC AGTGGAACAA CAAGAACAAC GCGTATGGAT ACATGGCGGA GAGCGAGGGG 
AGTATGGATG GAACACGGGT GATTAGCAGG AAGAAGCTGC AGAGGAAGCC AGTGGGCCTG 
GGCAGCACCG CACCTGCAGG CAGAGCCAAC AACTTCTCCA AGTCTGCCCG TCTAAAGTGG 
CAAACCCTTG AGCGTCGCAT CATGGACATC GTCATGCAGA GAATGACCAT CTCCAACGTG 
GAAGCAGACA TGGATCGCCT TATCAAGAAA CGAGAAGAGC TGACAATGCA GCAGGAAGCT 
CTCTCACATA AAAGGGAGAT ACTGATGGCC GACGGAGAGG GGCCAGAGGC GGAGGACCGC 
CTCCTTCAGG AAGTTAACGA GGAGATCGAG GTGCTGAATG CCAACATAGA CTACATCAAT 
GACAGTCTGT CGGACTGCCA GGCCACCATC GTGCAGATAG AGGAGACCAA GGATGAACTG 
GACTCGGTGG ATACCTCAGT GGTGATCAGC TCCTGTTCCC TGGCTGAAGC ACGCCACCTG 
CTGGATCACT TCCTGAAGGC TTCCATTGAT AAGAGCTTAC AGGTGGCTCA GAGGGAGGCG 
CAGGTACGAC TGCTCGAGGG TCAGCTGAGG CAGACTGATG TGATCGGCTC GTCTCACAAT 
CACATGATCC TCGACGCGCT GCGAGAAAAA GCAGAGTGCA TCCCTGAGCT CCAGGCTCTC 
ATCCACAACG TCCAGCAAGA AAATGGTTAC GCCAGCACGG ACGAGGAGCC CTCTGAGTTC 
AGCCAAGCCT CTGACAGCAG TGTTTCACAG ATGAAGGAAT CCAACAGCCA GGATGACTTT 
AAAGTAAAGA TGGAGCCTCG CCTCTCGGCC CAGATGAAGG CGGTGTCTGC GGAGTACCTC 
GGTCCCATCC TGGATCCCTC ATCAAGCAGC AAGCAGCAGC ACATCACTAA GTCCCTAGCC 
TCACTGACTG ACATCCAGGA GGATGGCTTA AGTCTCATTC CAGGAAGTCT GGCACTGAGC 
GTTCCCCTCC GGGACCCTTT TCACAGGGAA CGAGCGTCCC GCACAATCAG CCTTCCTGTC 
AGAGGACACA CTTTTCCCAG GCAGTCTCGA GGTTATGACA CTTCCCCCAT TACAAGAAGG 
AAATCTTACG ACCGTTCGTA CAGGCCCACT GATGTCTACA CTCCCCCCTC CTCACCTCCT 
TTAAGGACAA GGAATGATCG CAACGTCTTC TCCAGACTGA CCAGCAACCA AGCGCAAGGT 
TCTGCTCTGG ACAAGGGTGT GATCAATCCC ATTGGAGGTG TAAAGGGGGG TCGGACAGCA 
CCCCTCCAGT GTGTCTCTGT TGCCGAGGGC CACTCGAAGC CGGTTCTGTG TGTGGACGCT 
ACAGATGAGC TGCTTTTCAC TGGATCTAAA GATCGCACCT GTAAGATGTG GAATCTAGTC 
ACAGGTCAGG AGATTGCCAC CCTCAAAGGG CACCCAAACA ACGTGGTGTC GGTAAAATAT 
TGTCCCTCTT CTGGTCTGGT CTTCAGCGTC TCCACCTCAT ACATCAAGGT GTGGGACATC 
CGTGACTCTG CAAAGTGCAT ACGCACACTC ACGTCATCAG GGCAGGTAGT TACAGGAGAC 
GCGTGCGCTG GCACAACCAC CCGGACAATC ACCTTTGCTC AGGGCGAGTG TCAGATCAAC 
CAGATAGCGC TCAACCCATT AGGATCTGTG CTCTATGCTG CTGCTGGAAA TATTGTTCGG 
ATGTGGGACC TTAACAGGAT GCAGGCAACA GGCAAACTCT CAGGTCACAT CGGTTCTGTC 
ATGTGTCTGA CAGTGGGACA GTCTCTGCTG GGCAAAGACC AGGTCATCAC TGGCTCCAAA 
GACCATTACG TCAAGATATT TGATGTGGCA GAAGGAACTC TGGGTAATGT GGGTCCCGCT 
CATAACTTTG AGCCTCCGCA CTATGACGGC ATTGAATGTT TGGCCGTGCA GGGAGACGTG 
CTGTTCAGCG GCTCGCGAGA CAACGGTATC AAGAAGTGGG AGCTGGAGCA GCAGGAGCTC 
ACACAGCAAA TCCCGAATGC CCACAAGGAC TGGGTGTGCG CTCTGGCCTA TGTACCCGGC 
CGGCCCATGT TGCTGAGCGC CTGTCGGGGT GGCATGCTCA AGGTGTGGAA TGTGGAAAAC 
TTTACGCCCA TAGGAGAGGT CAGAGGTCAT GACAGCCCCA TTAATGCCAT ATGCACCAAC 
TCCAGGCATA TCTTTACAGC CTCCAGCGAC AAGACAGTGA AGATCTGGCT GTCAAAGGTG 
TGGAGGAAGA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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kif21b cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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