phka1 cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	phka1
Entrez Gene ID	102216420
Full Name	phosphorylase kinase regulatory subunit alpha 1
Gene Type	protein-coding
Organism	Xiphophorus maculatus(southern platyfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005806429.1	XP_005806486.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
XM_023345747.1	XP_023201515.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
XM_023345749.1	XP_023201517.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
XM_023345752.1	XP_023201520.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X5
XM_023345750.1	XP_023201518.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
XM_023345751.1	XP_023201519.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X4

Pathways from BioSystems
Gene Ontology

The following phka1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phka1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXi11745	XM_005806429.1 Latest version!	Xiphophorus maculatus phosphorylase kinase, alpha 1 (muscle) (phka1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXi23726	XM_023345747.1 Latest version!	Xiphophorus maculatus phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXi23727	XM_023345749.1 Latest version!	Xiphophorus maculatus phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXi23728	XM_023345750.1 Latest version!	Xiphophorus maculatus phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXi23729	XM_023345751.1 Latest version!	Xiphophorus maculatus phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXi23730	XM_023345752.1 Latest version!	Xiphophorus maculatus phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

phka1 ( XM_005806429.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_005806486.1 Xiphophorus maculatus phosphorylase kinase, alpha 1 (muscle) (phka1), mRNA.

Price & Availability↑

CloneID	OXi11745
Clone ID Related Accession (Same CDS sequence)	XM_005806429.1
Accession Version	XM_005806429.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1445702400000
Organism	Xiphophorus maculatus(Southern platyfish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005372341.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xiphophorus maculatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGAGAAGTC GGAGCAACTC TGGCGTTAAA TTGGACAACT ATGCCCGGAT AGTGCAGGAA 
ACTATCCTGC AGCACCAAGA TCCGGTGACG GGTCTTCTGC CAGGCAGCAC CGACCAGCCG 
GACGCCTGGG TCAGAGACAA TGTGTACAGC ATTGCGTCCG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAGA ACGCAGACAG AGACGAAGAC AAGGCCAAGG CCTATGAGCT GGAACAGAGC 
GTGGTGAAGT TGATGAGAGG TGTTCTCCAG TGTATAATGA GGCAGCTGGA CAAAGTGGAG 
AAGTTTAAAT ACAGCCGGAG CACTAAAGAC TCGCTCCACG CCAAGTACAA CACCAAGACC 
TGCGCCACCG TCGTCGGCGA CGACCAGTGG GGCCACCTGC AGGTGGACGC CACCTCTCTG 
TTCCTGCTCT TCCTCGCTCA GATGACTGCA TCAGGTCTCC ATATCATCTA CACCCAAGAC 
GAAGTGGATG TCATTCAGAA TCTCATGTTC TACATCGAGG CAGCGTACAA AGTGGCTGAC 
TATGGGATGT GGGAGAGAGG AGACAAAACC AACCAGGGCA TCACAGAGAT CAACGCCAGC 
TCTATAGGCA CGGCTAAGGC AGCTTTGGAG GCTCTGGATG AACTTAACCT GTTTGGAGCA 
AAAGGAGGGC CTGGATCTGT GGTCCATGCT CTGGCCGATG ACATACAGCA CTGTCAGTCT 
ATTCTGACGT CAATGTTACC CAGAGCCTCC ATCTCTAAAG AGGTAGACGC CGGAGTCCTG 
GCCATCATCA CCTACCCCGC CTTCGCTGTG GAGGACATGA GCATAGTGAA CATGACGAAG 
GAGGAGATCA TCTCTAAACT GCAGGGTCGA TACGGCTGCT GCAGGTTTCT CAGAGACGGC 
CACAGGACTC CCCGAGAGGA TCCAAACCGG CTGTATTACG AGTCCGCTGA GCTGAAGCTG 
TTTGAAAACA TAGAGTGCGA GTGGCCTCTG TTTTGGACGT ATCTCATACT CGACGGCATT 
TTTAGCAACA GCCATGAGCA GGTGCTGGAG TACCAGGAGG CGCTGGAGGC GATCCTGATC 
AAACAGAAAG ATGGGATCCG GCTGCTGCCA GAACTCTACA GTGTCCCTCC AGATAAGGTG 
GAAGAGGAGT ACATGAACCC TCACTCAGTA GAAAGGATCC CAATGGGGAA GTGTCCTCTA 
AAATGGGGTC AATCGTTGTA CATCCTTGGA AAACTCCTCT CTGAGGGTTT TCTCGCCCCC 
GGAGAGATCG ATCCCCTTAA CAGACGGTTC TCCACGATCC CTAAGCCTGA TGTAGTTGTA 
CAGGTGTCCA TTTTGGCTGA GACGGATGAG ATTAAGGGGC TGCTGATGAA GAATGGTATA 
AACGTGGAGA CCGTGGCGGA TATCCATCCC ATCCATGTTC AGCCCTCCAG AGTCCTGAGC 
CACATCTATG CCAGACTCGG CCGTAACCCA AGGCTCGGCC TTACAGGGAG ACCCTACAGA 
AGAATAGGAG TCCTGGGAAC CTCCAAGTTC TACATCATTA GAAACACAAT CTTTGCTTTC 
ACTCCTCAGT TCATCGACCA CCAGCAGTTC TACTTGGCTC TGGACAACAA GATGATCGTG 
GAGATGCTGA GGAACGAGAT TGCCTACCTT GCGTCCAGAT GGAGGATAAC CGGACGACCG 
ACGGTCACCT TCCCCATCTC ACAGACCATG CTGACTGAAG ACCGCACCGA CCTGGACCCT 
GCAGTCATAG CTATGCTAAA GAAGCTACAG GACGGATATT ACGGAGGAGC AAGGACCCAG 
ACGGGAAAGC TGTCGGAGTT CCTGACTACT TCCTGCTGCG CTCACCTCAG CTTCCTCGAC 
GGTAAGGGTT CCGGCAGCAT GGGCCGGCAC GGCGACCAGT ATGATGATGA TGATGACGGC 
GATGGATACA TGCATGAATT GCGTTGTGAT GATGCGGACG ACCTCGCCCA GTATCTGGAC 
CACCTGTTGG CGCACTCTTC CCCCAAGAAG TCTAAGCGTC AGCCAGGAGG CCTGGGCAGG 
TTCAAGGCGG CGGCCACCAA GACCAAAGAG ATGGTTTCTC TGATGAACAA GGCTCAGGAT 
CTGAACGTAC ACAACGTCAA CATGTACCTA CCGAGCAAGC TGTTTCATTC CTGTCAGCCG 
TCACTCAACC TGAATCTCCC AGACCCTTCT GAGCCGCAGG AAACGGAGGT TCTCCCTAGC 
TCGGCGGTGT CGGTCACAGC GGAGAGCGGC ATCCCCAGAG ACGAAAGCGG AGCCATCGAC 
TACGACGCTC TGGTGCAGCT GCTGAAGGAC ACGAAAAACC TCCAGGACCA GGCAGATATC 
CTTTACATCC TCTTCAAAGA CAAGGGCATG GAGTGGGACA CCCAGCTGCA AGGCAAAGGC 
TCCACAGTCA GATCTCTACT GAGCGAGCTT TATGAGAAGG CTGGTGACCT CAAACACTGG 
GGCCTCGTCA GGATGATCTC TGGGATGCTG AAGAAGAAGG TGGAGGAGCT CGACTCGGCT 
TGCTCTGACT TGCTGGCGCA CCAGAAGCAC CTGACCGTGG GACTTCCACC TGAGCCGAGA 
GAGAAGACCA TCACGGCTCC CATCCCTCCG GACCAGCTGG CCGCTCTCAT CGACGAGGCC 
AGCGACAACA ACATCAGCTT GGCCATCCTC ACCCAGGAGA TCATGGTGTA CCTGGCGATG 
AGCATCAGGA CTCAGCCGTC TCTGTTCAGC GAGATGTTCC GGCTGCGGAT CGGCCTCATT 
ATTCAGGTCA TGGCTACCGA ACTGGCTCAG TCACTCAGCT GCTCAGGAGA GGAAGCCACA 
GAGAGCCTGA TGAGTCTCAG CCCGTCTGAG CTGAAGAATC TGCTGCACCA CATCCTGAGT 
GGGAAAGAGT TCGGAGTGCA GCGCAGCGTA AGGGAGGCAG ACGTCGGCGT CAGTCCTGCC 
ATCTCCATCC ATCACCTAGG CAACACCGGC GCCACCAAGA GCGAGAGAGC AGGCATCAGT 
AAACTGAAGA GCGACATGAA GATGCTCGAC CAGCCTGTGA CGCCCACTGA GAAGGGTATC 
CGAGCTCAGT CAATGGACGT AGAAAGCATC GAATCTGGGA GGTACAGACT GTCCTCTGTA 
GAGTCTATTG ACGTTCCGGA CAGCTTCCCT GTTTCCAAGG ACACCAGACA TGGCCAGTGG 
CTGCGCAGGA GGCGGCTGGA CGGAGCGCTG AACAGGGTCC CCGTTGGCTT CTACCAGAAA 
GTGTGGAAGA TCCTGCAGAA GTGCCATGGT CTGTCCATAG AGGGCTTTGT CCTTCCATCT 
TCAACCACCA GAGAGATGAC ACCTGGAGAG ATTAAGTTCT CCGTCCACGT GGAGACGGTG 
CTGAACCGTG TCCCCCAGCC CGAGTACCGA CAGCTGCTGG TGGAGGCCAT CCTGGTCCTC 
ACCATGCTGG CCGACGTGGA CATCCCCAGC ATCGGCTCCA TCATTCACGT GGAAAAGATC 
GTCCACCAGG CCAACGACAT GTTCTACAAA GATCAGAAGG ACCTGGGAGC GGAAGAGCAC 
ATCCTGGAGA AGGACCCGTT CACGGGAGTG TGCAAGCTGT TGTACGACAG CGCTCCCAGT 
GGCCGCTTTG GGAGCATGAC CTACCTCACT AAGGCTGTGG CGGTGTATGT GCAGGACTTC 
CTGCCCAGCG GGGCCTGTGC GGTGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005806486.1
CDS	1..3690
Translation	MRSRSNSGVKLDNYARIVQETILQHQDPVTGLLPGSTDQPDAWVRDNVYSIASVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSRSTKDSLHAKYNTKTCATVVGDDQWGHLQVDATSLFLLFLAQMTASGLHIIYTQDEVDVIQNLMFYIEAAYKVADYGMWERGDKTNQGITEINASSIGTAKAALEALDELNLFGAKGGPGSVVHALADDIQHCQSILTSMLPRASISKEVDAGVLAIITYPAFAVEDMSIVNMTKEEIISKLQGRYGCCRFLRDGHRTPREDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFSNSHEQVLEYQEALEAILIKQKDGIRLLPELYSVPPDKVEEEYMNPHSVERIPMGKCPLKWGQSLYILGKLLSEGFLAPGEIDPLNRRFSTIPKPDVVVQVSILAETDEIKGLLMKNGINVETVADIHPIHVQPSRVLSHIYARLGRNPRLGLTGRPYRRIGVLGTSKFYIIRNTIFAFTPQFIDHQQFYLALDNKMIVEMLRNEIAYLASRWRITGRPTVTFPISQTMLTEDRTDLDPAVIAMLKKLQDGYYGGARTQTGKLSEFLTTSCCAHLSFLDGKGSGSMGRHGDQYDDDDDGDGYMHELRCDDADDLAQYLDHLLAHSSPKKSKRQPGGLGRFKAAATKTKEMVSLMNKAQDLNVHNVNMYLPSKLFHSCQPSLNLNLPDPSEPQETEVLPSSAVSVTAESGIPRDESGAIDYDALVQLLKDTKNLQDQADILYILFKDKGMEWDTQLQGKGSTVRSLLSELYEKAGDLKHWGLVRMISGMLKKKVEELDSACSDLLAHQKHLTVGLPPEPREKTITAPIPPDQLAALIDEASDNNISLAILTQEIMVYLAMSIRTQPSLFSEMFRLRIGLIIQVMATELAQSLSCSGEEATESLMSLSPSELKNLLHHILSGKEFGVQRSVREADVGVSPAISIHHLGNTGATKSERAGISKLKSDMKMLDQPVTPTEKGIRAQSMDVESIESGRYRLSSVESIDVPDSFPVSKDTRHGQWLRRRRLDGALNRVPVGFYQKVWKILQKCHGLSIEGFVLPSSTTREMTPGEIKFSVHVETVLNRVPQPEYRQLLVEAILVLTMLADVDIPSIGSIIHVEKIVHQANDMFYKDQKDLGAEEHILEKDPFTGVCKLLYDSAPSGRFGSMTYLTKAVAVYVQDFLPSGACAVQ

Target ORF information:

RefSeq Version	XM_005806429.1
Organism	Xiphophorus maculatus(Southern platyfish)
Definition	Xiphophorus maculatus phosphorylase kinase, alpha 1 (muscle) (phka1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005806429.1

ORF Insert Sequence:

ATGAGAAGTC GGAGCAACTC TGGCGTTAAA TTGGACAACT ATGCCCGGAT AGTGCAGGAA 
ACTATCCTGC AGCACCAAGA TCCGGTGACG GGTCTTCTGC CAGGCAGCAC CGACCAGCCG 
GACGCCTGGG TCAGAGACAA TGTGTACAGC ATTGCGTCCG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAGA ACGCAGACAG AGACGAAGAC AAGGCCAAGG CCTATGAGCT GGAACAGAGC 
GTGGTGAAGT TGATGAGAGG TGTTCTCCAG TGTATAATGA GGCAGCTGGA CAAAGTGGAG 
AAGTTTAAAT ACAGCCGGAG CACTAAAGAC TCGCTCCACG CCAAGTACAA CACCAAGACC 
TGCGCCACCG TCGTCGGCGA CGACCAGTGG GGCCACCTGC AGGTGGACGC CACCTCTCTG 
TTCCTGCTCT TCCTCGCTCA GATGACTGCA TCAGGTCTCC ATATCATCTA CACCCAAGAC 
GAAGTGGATG TCATTCAGAA TCTCATGTTC TACATCGAGG CAGCGTACAA AGTGGCTGAC 
TATGGGATGT GGGAGAGAGG AGACAAAACC AACCAGGGCA TCACAGAGAT CAACGCCAGC 
TCTATAGGCA CGGCTAAGGC AGCTTTGGAG GCTCTGGATG AACTTAACCT GTTTGGAGCA 
AAAGGAGGGC CTGGATCTGT GGTCCATGCT CTGGCCGATG ACATACAGCA CTGTCAGTCT 
ATTCTGACGT CAATGTTACC CAGAGCCTCC ATCTCTAAAG AGGTAGACGC CGGAGTCCTG 
GCCATCATCA CCTACCCCGC CTTCGCTGTG GAGGACATGA GCATAGTGAA CATGACGAAG 
GAGGAGATCA TCTCTAAACT GCAGGGTCGA TACGGCTGCT GCAGGTTTCT CAGAGACGGC 
CACAGGACTC CCCGAGAGGA TCCAAACCGG CTGTATTACG AGTCCGCTGA GCTGAAGCTG 
TTTGAAAACA TAGAGTGCGA GTGGCCTCTG TTTTGGACGT ATCTCATACT CGACGGCATT 
TTTAGCAACA GCCATGAGCA GGTGCTGGAG TACCAGGAGG CGCTGGAGGC GATCCTGATC 
AAACAGAAAG ATGGGATCCG GCTGCTGCCA GAACTCTACA GTGTCCCTCC AGATAAGGTG 
GAAGAGGAGT ACATGAACCC TCACTCAGTA GAAAGGATCC CAATGGGGAA GTGTCCTCTA 
AAATGGGGTC AATCGTTGTA CATCCTTGGA AAACTCCTCT CTGAGGGTTT TCTCGCCCCC 
GGAGAGATCG ATCCCCTTAA CAGACGGTTC TCCACGATCC CTAAGCCTGA TGTAGTTGTA 
CAGGTGTCCA TTTTGGCTGA GACGGATGAG ATTAAGGGGC TGCTGATGAA GAATGGTATA 
AACGTGGAGA CCGTGGCGGA TATCCATCCC ATCCATGTTC AGCCCTCCAG AGTCCTGAGC 
CACATCTATG CCAGACTCGG CCGTAACCCA AGGCTCGGCC TTACAGGGAG ACCCTACAGA 
AGAATAGGAG TCCTGGGAAC CTCCAAGTTC TACATCATTA GAAACACAAT CTTTGCTTTC 
ACTCCTCAGT TCATCGACCA CCAGCAGTTC TACTTGGCTC TGGACAACAA GATGATCGTG 
GAGATGCTGA GGAACGAGAT TGCCTACCTT GCGTCCAGAT GGAGGATAAC CGGACGACCG 
ACGGTCACCT TCCCCATCTC ACAGACCATG CTGACTGAAG ACCGCACCGA CCTGGACCCT 
GCAGTCATAG CTATGCTAAA GAAGCTACAG GACGGATATT ACGGAGGAGC AAGGACCCAG 
ACGGGAAAGC TGTCGGAGTT CCTGACTACT TCCTGCTGCG CTCACCTCAG CTTCCTCGAC 
GGTAAGGGTT CCGGCAGCAT GGGCCGGCAC GGCGACCAGT ATGATGATGA TGATGACGGC 
GATGGATACA TGCATGAATT GCGTTGTGAT GATGCGGACG ACCTCGCCCA GTATCTGGAC 
CACCTGTTGG CGCACTCTTC CCCCAAGAAG TCTAAGCGTC AGCCAGGAGG CCTGGGCAGG 
TTCAAGGCGG CGGCCACCAA GACCAAAGAG ATGGTTTCTC TGATGAACAA GGCTCAGGAT 
CTGAACGTAC ACAACGTCAA CATGTACCTA CCGAGCAAGC TGTTTCATTC CTGTCAGCCG 
TCACTCAACC TGAATCTCCC AGACCCTTCT GAGCCGCAGG AAACGGAGGT TCTCCCTAGC 
TCGGCGGTGT CGGTCACAGC GGAGAGCGGC ATCCCCAGAG ACGAAAGCGG AGCCATCGAC 
TACGACGCTC TGGTGCAGCT GCTGAAGGAC ACGAAAAACC TCCAGGACCA GGCAGATATC 
CTTTACATCC TCTTCAAAGA CAAGGGCATG GAGTGGGACA CCCAGCTGCA AGGCAAAGGC 
TCCACAGTCA GATCTCTACT GAGCGAGCTT TATGAGAAGG CTGGTGACCT CAAACACTGG 
GGCCTCGTCA GGATGATCTC TGGGATGCTG AAGAAGAAGG TGGAGGAGCT CGACTCGGCT 
TGCTCTGACT TGCTGGCGCA CCAGAAGCAC CTGACCGTGG GACTTCCACC TGAGCCGAGA 
GAGAAGACCA TCACGGCTCC CATCCCTCCG GACCAGCTGG CCGCTCTCAT CGACGAGGCC 
AGCGACAACA ACATCAGCTT GGCCATCCTC ACCCAGGAGA TCATGGTGTA CCTGGCGATG 
AGCATCAGGA CTCAGCCGTC TCTGTTCAGC GAGATGTTCC GGCTGCGGAT CGGCCTCATT 
ATTCAGGTCA TGGCTACCGA ACTGGCTCAG TCACTCAGCT GCTCAGGAGA GGAAGCCACA 
GAGAGCCTGA TGAGTCTCAG CCCGTCTGAG CTGAAGAATC TGCTGCACCA CATCCTGAGT 
GGGAAAGAGT TCGGAGTGCA GCGCAGCGTA AGGGAGGCAG ACGTCGGCGT CAGTCCTGCC 
ATCTCCATCC ATCACCTAGG CAACACCGGC GCCACCAAGA GCGAGAGAGC AGGCATCAGT 
AAACTGAAGA GCGACATGAA GATGCTCGAC CAGCCTGTGA CGCCCACTGA GAAGGGTATC 
CGAGCTCAGT CAATGGACGT AGAAAGCATC GAATCTGGGA GGTACAGACT GTCCTCTGTA 
GAGTCTATTG ACGTTCCGGA CAGCTTCCCT GTTTCCAAGG ACACCAGACA TGGCCAGTGG 
CTGCGCAGGA GGCGGCTGGA CGGAGCGCTG AACAGGGTCC CCGTTGGCTT CTACCAGAAA 
GTGTGGAAGA TCCTGCAGAA GTGCCATGGT CTGTCCATAG AGGGCTTTGT CCTTCCATCT 
TCAACCACCA GAGAGATGAC ACCTGGAGAG ATTAAGTTCT CCGTCCACGT GGAGACGGTG 
CTGAACCGTG TCCCCCAGCC CGAGTACCGA CAGCTGCTGG TGGAGGCCAT CCTGGTCCTC 
ACCATGCTGG CCGACGTGGA CATCCCCAGC ATCGGCTCCA TCATTCACGT GGAAAAGATC 
GTCCACCAGG CCAACGACAT GTTCTACAAA GATCAGAAGG ACCTGGGAGC GGAAGAGCAC 
ATCCTGGAGA AGGACCCGTT CACGGGAGTG TGCAAGCTGT TGTACGACAG CGCTCCCAGT 
GGCCGCTTTG GGAGCATGAC CTACCTCACT AAGGCTGTGG CGGTGTATGT GCAGGACTTC 
CTGCCCAGCG GGGCCTGTGC GGTGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1 ( XM_023345747.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023201515.1 Xiphophorus maculatus phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXi23726
Clone ID Related Accession (Same CDS sequence)	XM_023345747.1
Accession Version	XM_023345747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036456.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGTC GGAGCAACTC TGGCGTTAAA TTGGACAACT ATGCCCGGAT AGTGCAGGAA 
ACTATCCTGC AGCACCAAGA TCCGGTGACG GGTCTTCTGC CAGGCAGCAC CGACCAGCCG 
GACGCCTGGG TCAGAGACAA TGTGTACAGC ATTGCGTCCG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAGA ACGCAGACAG AGACGAAGAC AAGGCCAAGG CCTATGAGCT GGAACAGAGC 
GTGGTGAAGT TGATGAGAGG TGTTCTCCAG TGTATAATGA GGCAGCTGGA CAAAGTGGAG 
AAGTTTAAAT ACAGCCGGAG CACTAAAGAC TCGCTCCACG CCAAGTACAA CACCAAGACC 
TGCGCCACCG TCGTCGGCGA CGACCAGTGG GGCCACCTGC AGGTGGACGC CACCTCTCTG 
TTCCTGCTCT TCCTCGCTCA GATGACTGCA TCAGGTCTCC ATATCATCTA CACCCAAGAC 
GAAGTGGATG TCATTCAGAA TCTCATGTTC TACATCGAGG CAGCGTACAA AGTGGCTGAC 
TATGGGATGT GGGAGAGAGG AGACAAAACC AACCAGGGCA TCACAGAGAT CAACGCCAGC 
TCTATAGGCA CGGCTAAGGC AGCTTTGGAG GCTCTGGATG AACTTAACCT GTTTGGAGCA 
AAAGGAGGGC CTGGATCTGT GGTCCATGCT CTGGCCGATG ACATACAGCA CTGTCAGTCT 
ATTCTGACGT CAATGTTACC CAGAGCCTCC ATCTCTAAAG AGGTAGACGC CGGAGTCCTG 
GCCATCATCA CCTACCCCGC CTTCGCTGTG GAGGACATGA GCATAGTGAA CATGACGAAG 
GAGGAGATCA TCTCTAAACT GCAGGGTCGA TACGGCTGCT GCAGGTTTCT CAGAGACGGC 
CACAGGACTC CCCGAGAGGA TCCAAACCGG CTGTATTACG AGTCCGCTGA GCTGAAGCTG 
TTTGAAAACA TAGAGTGCGA GTGGCCTCTG TTTTGGACGT ATCTCATACT CGACGGCATT 
TTTAGCAACA GCCATGAGCA GGTGCTGGAG TACCAGGAGG CGCTGGAGGC GATCCTGATC 
AAACAGAAAG ATGGGATCCG GCTGCTGCCA GAACTCTACA GTGTCCCTCC AGATAAGGTG 
GAAGAGGAGT ACATGAACCC TCACTCAGTA GAAAGGATCC CAATGGGGAA GTGTCCTCTA 
AAATGGGGTC AATCGTTGTA CATCCTTGGA AAACTCCTCT CTGAGGGTTT TCTCGCCCCC 
GGAGAGATCG ATCCCCTTAA CAGACGGTTC TCCACGATCC CTAAGCCTGA TGTAGTTGTA 
CAGGTGTCCA TTTTGGCTGA GACGGATGAG ATTAAGGGGC TGCTGATGAA GAATGGTATA 
AACGTGGAGA CCGTGGCGGA TATCCATCCC ATCCATGTTC AGCCCTCCAG AGTCCTGAGC 
CACATCTATG CCAGACTCGG CCGTAACCCA AGGCTCGGCC TTACAGGGAG ACCCTACAGA 
AGAATAGGAG TCCTGGGAAC CTCCAAGTTC TACATCATTA GAAACACAAT CTTTGCTTTC 
ACTCCTCAGT TCATCGACCA CCAGCAGTTC TACTTGGCTC TGGACAACAA GATGATCGTG 
GAGATGCTGA GGAACGAGAT TGCCTACCTT GCGTCCAGAT GGAGGATAAC CGGACGACCG 
ACGGTCACCT TCCCCATCTC ACAGACCATG CTGACTGAAG ACCGCACCGA CCTGGACCCT 
GCAGTCATAG CTATGCTAAA GAAGCTACAG GACGGATATT ACGGAGGAGC AAGGACCCAG 
ACGGGAAAGC TGTCGGAGTT CCTGACTACT TCCTGCTGCG CTCACCTCAG CTTCCTCGAC 
GGTAAGGGTT CCGGCAGCAT GGGCCGGCAC GGCGACCAGT ATGATGATGA TGATGACGGC 
GATGGATACA TGCATGAATT GCGTTGTGAT GATGAAGCGG ACGACCTCGC CCAGTATCTG 
GACCACCTGT TGGCGCACTC TTCCCCCAAG AAGTCTAAGC GTCAGCCAGG AGGCCTGGGC 
AGGTTCAAGG CGGCGGCCAC CAAGACCAAA GAGATGGTTT CTCTGATGAA CAAGGCTCAG 
GATCTGAACG TACACAACGT CAACATGTAC CTACCGAGCA AGCTGTTTCA TTCCTGTCAG 
CCGTCACTCA ACCTGAATCT CCCAGACCCT TCTGAGCCGC AGGAAACGGA GAGCTCGGCG 
GTGTCGGTCA CAGCGGAGAG CGGCATCCCC AGAGACGAAA GCGGAGCCAT CGACTACGAC 
GCTCTGGTGC AGCTGCTGAA GGACACGAAA AACCTCCAGG ACCAGGCAGA TATCCTTTAC 
ATCCTCTTCA AAGACAAGGG CATGGAGTGG GACACCCAGC TGCAAGGCAA AGGCTCCACA 
GTCAGATCTC TACTGAGCGA GCTTTATGAG AAGGCTGGTG ACCTCAAACA CTGGGGCCTC 
GTCAGGATGA TCTCTGGGAT GCTGAAGAAG AAGGTGGAGG AGCTCGACTC GGCTTGCTCT 
GACTTGCTGG CGCACCAGAA GCACCTGACC GTGGGACTTC CACCTGAGCC GAGAGAGAAG 
ACCATCACGG CTCCCATCCC TCCGGACCAG CTGGCCGCTC TCATCGACGA GGCCAGCGAC 
AACAACATCA GCTTGGCCAT CCTCACCCAG GAGATCATGG TGTACCTGGC GATGAGCATC 
AGGACTCAGC CGTCTCTGTT CAGCGAGATG TTCCGGCTGC GGATCGGCCT CATTATTCAG 
GTCATGGCTA CCGAACTGGC TCAGTCACTC AGCTGCTCAG GAGAGGAAGC CACAGAGAGC 
CTGATGAGTC TCAGCCCGTC TGAGCTGAAG AATCTGCTGC ACCACATCCT GAGTGGGAAA 
GAGTTCGGAG TGCAGCGCAG CGTAAGGGAG GCAGACGTCG GCGTCAGTCC TGCCATCTCC 
ATCCATCACC TAGGCAACAC CGGCGCCACC AAGAGCGAGA GAGCAGGCAT CAGTAAACTG 
AAGAGCGACA TGAAGATGCT GGAGCGCCGG TTGTCTATGC TGGACCCCAG TAAGCTCGAC 
CAGCCTGTGA CGCCCACTGA GAAGGGTATC CGAGCTCAGT CAATGGACGT AGAAAGCATC 
GAATCTGGGA GGTACAGACT GTCCTCTGTA GAGTCTATTG ACGTTCCGGA CAGCTTCCCT 
GTTTCCAAGG ACACCAGACA TGGCCAGTGG CTGCGCAGGA GGCGGCTGGA CGGAGCGCTG 
AACAGGGTCC CCGTTGGCTT CTACCAGAAA GTGTGGAAGA TCCTGCAGAA GTGCCATGGT 
CTGTCCATAG AGGGCTTTGT CCTTCCATCT TCAACCACCA GAGAGATGAC ACCTGGAGAG 
ATTAAGTTCT CCGTCCACGT GGAGACGGTG CTGAACCGTG TCCCCCAGCC CGAGTACCGA 
CAGCTGCTGG TGGAGGCCAT CCTGGTCCTC ACCATGCTGG CCGACGTGGA CATCCCCAGC 
ATCGGCTCCA TCATTCACGT GGAAAAGATC GTCCACCAGG CCAACGACAT GTTCTACAAA 
GATCAGAAGG ACCTGGGAGC GGAAGAGCAC ATCCTGGAGA AGGACCCGTT CACGGGAGTG 
TGCAAGCTGT TGTACGACAG CGCTCCCAGT GGCCGCTTTG GGAGCATGAC CTACCTCACT 
AAGGCTGTGG CGGTGTATGT GCAGGACTTC CTGCCCAGCG GGGCCTGTGC GGTGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023201515.1
CDS	193..3912
Translation	MRSRSNSGVKLDNYARIVQETILQHQDPVTGLLPGSTDQPDAWVRDNVYSIASVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSRSTKDSLHAKYNTKTCATVVGDDQWGHLQVDATSLFLLFLAQMTASGLHIIYTQDEVDVIQNLMFYIEAAYKVADYGMWERGDKTNQGITEINASSIGTAKAALEALDELNLFGAKGGPGSVVHALADDIQHCQSILTSMLPRASISKEVDAGVLAIITYPAFAVEDMSIVNMTKEEIISKLQGRYGCCRFLRDGHRTPREDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFSNSHEQVLEYQEALEAILIKQKDGIRLLPELYSVPPDKVEEEYMNPHSVERIPMGKCPLKWGQSLYILGKLLSEGFLAPGEIDPLNRRFSTIPKPDVVVQVSILAETDEIKGLLMKNGINVETVADIHPIHVQPSRVLSHIYARLGRNPRLGLTGRPYRRIGVLGTSKFYIIRNTIFAFTPQFIDHQQFYLALDNKMIVEMLRNEIAYLASRWRITGRPTVTFPISQTMLTEDRTDLDPAVIAMLKKLQDGYYGGARTQTGKLSEFLTTSCCAHLSFLDGKGSGSMGRHGDQYDDDDDGDGYMHELRCDDEADDLAQYLDHLLAHSSPKKSKRQPGGLGRFKAAATKTKEMVSLMNKAQDLNVHNVNMYLPSKLFHSCQPSLNLNLPDPSEPQETESSAVSVTAESGIPRDESGAIDYDALVQLLKDTKNLQDQADILYILFKDKGMEWDTQLQGKGSTVRSLLSELYEKAGDLKHWGLVRMISGMLKKKVEELDSACSDLLAHQKHLTVGLPPEPREKTITAPIPPDQLAALIDEASDNNISLAILTQEIMVYLAMSIRTQPSLFSEMFRLRIGLIIQVMATELAQSLSCSGEEATESLMSLSPSELKNLLHHILSGKEFGVQRSVREADVGVSPAISIHHLGNTGATKSERAGISKLKSDMKMLERRLSMLDPSKLDQPVTPTEKGIRAQSMDVESIESGRYRLSSVESIDVPDSFPVSKDTRHGQWLRRRRLDGALNRVPVGFYQKVWKILQKCHGLSIEGFVLPSSTTREMTPGEIKFSVHVETVLNRVPQPEYRQLLVEAILVLTMLADVDIPSIGSIIHVEKIVHQANDMFYKDQKDLGAEEHILEKDPFTGVCKLLYDSAPSGRFGSMTYLTKAVAVYVQDFLPSGACAVQ

Target ORF information:

RefSeq Version	XM_023345747.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023345747.1

ORF Insert Sequence:

ATGAGAAGTC GGAGCAACTC TGGCGTTAAA TTGGACAACT ATGCCCGGAT AGTGCAGGAA 
ACTATCCTGC AGCACCAAGA TCCGGTGACG GGTCTTCTGC CAGGCAGCAC CGACCAGCCG 
GACGCCTGGG TCAGAGACAA TGTGTACAGC ATTGCGTCCG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAGA ACGCAGACAG AGACGAAGAC AAGGCCAAGG CCTATGAGCT GGAACAGAGC 
GTGGTGAAGT TGATGAGAGG TGTTCTCCAG TGTATAATGA GGCAGCTGGA CAAAGTGGAG 
AAGTTTAAAT ACAGCCGGAG CACTAAAGAC TCGCTCCACG CCAAGTACAA CACCAAGACC 
TGCGCCACCG TCGTCGGCGA CGACCAGTGG GGCCACCTGC AGGTGGACGC CACCTCTCTG 
TTCCTGCTCT TCCTCGCTCA GATGACTGCA TCAGGTCTCC ATATCATCTA CACCCAAGAC 
GAAGTGGATG TCATTCAGAA TCTCATGTTC TACATCGAGG CAGCGTACAA AGTGGCTGAC 
TATGGGATGT GGGAGAGAGG AGACAAAACC AACCAGGGCA TCACAGAGAT CAACGCCAGC 
TCTATAGGCA CGGCTAAGGC AGCTTTGGAG GCTCTGGATG AACTTAACCT GTTTGGAGCA 
AAAGGAGGGC CTGGATCTGT GGTCCATGCT CTGGCCGATG ACATACAGCA CTGTCAGTCT 
ATTCTGACGT CAATGTTACC CAGAGCCTCC ATCTCTAAAG AGGTAGACGC CGGAGTCCTG 
GCCATCATCA CCTACCCCGC CTTCGCTGTG GAGGACATGA GCATAGTGAA CATGACGAAG 
GAGGAGATCA TCTCTAAACT GCAGGGTCGA TACGGCTGCT GCAGGTTTCT CAGAGACGGC 
CACAGGACTC CCCGAGAGGA TCCAAACCGG CTGTATTACG AGTCCGCTGA GCTGAAGCTG 
TTTGAAAACA TAGAGTGCGA GTGGCCTCTG TTTTGGACGT ATCTCATACT CGACGGCATT 
TTTAGCAACA GCCATGAGCA GGTGCTGGAG TACCAGGAGG CGCTGGAGGC GATCCTGATC 
AAACAGAAAG ATGGGATCCG GCTGCTGCCA GAACTCTACA GTGTCCCTCC AGATAAGGTG 
GAAGAGGAGT ACATGAACCC TCACTCAGTA GAAAGGATCC CAATGGGGAA GTGTCCTCTA 
AAATGGGGTC AATCGTTGTA CATCCTTGGA AAACTCCTCT CTGAGGGTTT TCTCGCCCCC 
GGAGAGATCG ATCCCCTTAA CAGACGGTTC TCCACGATCC CTAAGCCTGA TGTAGTTGTA 
CAGGTGTCCA TTTTGGCTGA GACGGATGAG ATTAAGGGGC TGCTGATGAA GAATGGTATA 
AACGTGGAGA CCGTGGCGGA TATCCATCCC ATCCATGTTC AGCCCTCCAG AGTCCTGAGC 
CACATCTATG CCAGACTCGG CCGTAACCCA AGGCTCGGCC TTACAGGGAG ACCCTACAGA 
AGAATAGGAG TCCTGGGAAC CTCCAAGTTC TACATCATTA GAAACACAAT CTTTGCTTTC 
ACTCCTCAGT TCATCGACCA CCAGCAGTTC TACTTGGCTC TGGACAACAA GATGATCGTG 
GAGATGCTGA GGAACGAGAT TGCCTACCTT GCGTCCAGAT GGAGGATAAC CGGACGACCG 
ACGGTCACCT TCCCCATCTC ACAGACCATG CTGACTGAAG ACCGCACCGA CCTGGACCCT 
GCAGTCATAG CTATGCTAAA GAAGCTACAG GACGGATATT ACGGAGGAGC AAGGACCCAG 
ACGGGAAAGC TGTCGGAGTT CCTGACTACT TCCTGCTGCG CTCACCTCAG CTTCCTCGAC 
GGTAAGGGTT CCGGCAGCAT GGGCCGGCAC GGCGACCAGT ATGATGATGA TGATGACGGC 
GATGGATACA TGCATGAATT GCGTTGTGAT GATGAAGCGG ACGACCTCGC CCAGTATCTG 
GACCACCTGT TGGCGCACTC TTCCCCCAAG AAGTCTAAGC GTCAGCCAGG AGGCCTGGGC 
AGGTTCAAGG CGGCGGCCAC CAAGACCAAA GAGATGGTTT CTCTGATGAA CAAGGCTCAG 
GATCTGAACG TACACAACGT CAACATGTAC CTACCGAGCA AGCTGTTTCA TTCCTGTCAG 
CCGTCACTCA ACCTGAATCT CCCAGACCCT TCTGAGCCGC AGGAAACGGA GAGCTCGGCG 
GTGTCGGTCA CAGCGGAGAG CGGCATCCCC AGAGACGAAA GCGGAGCCAT CGACTACGAC 
GCTCTGGTGC AGCTGCTGAA GGACACGAAA AACCTCCAGG ACCAGGCAGA TATCCTTTAC 
ATCCTCTTCA AAGACAAGGG CATGGAGTGG GACACCCAGC TGCAAGGCAA AGGCTCCACA 
GTCAGATCTC TACTGAGCGA GCTTTATGAG AAGGCTGGTG ACCTCAAACA CTGGGGCCTC 
GTCAGGATGA TCTCTGGGAT GCTGAAGAAG AAGGTGGAGG AGCTCGACTC GGCTTGCTCT 
GACTTGCTGG CGCACCAGAA GCACCTGACC GTGGGACTTC CACCTGAGCC GAGAGAGAAG 
ACCATCACGG CTCCCATCCC TCCGGACCAG CTGGCCGCTC TCATCGACGA GGCCAGCGAC 
AACAACATCA GCTTGGCCAT CCTCACCCAG GAGATCATGG TGTACCTGGC GATGAGCATC 
AGGACTCAGC CGTCTCTGTT CAGCGAGATG TTCCGGCTGC GGATCGGCCT CATTATTCAG 
GTCATGGCTA CCGAACTGGC TCAGTCACTC AGCTGCTCAG GAGAGGAAGC CACAGAGAGC 
CTGATGAGTC TCAGCCCGTC TGAGCTGAAG AATCTGCTGC ACCACATCCT GAGTGGGAAA 
GAGTTCGGAG TGCAGCGCAG CGTAAGGGAG GCAGACGTCG GCGTCAGTCC TGCCATCTCC 
ATCCATCACC TAGGCAACAC CGGCGCCACC AAGAGCGAGA GAGCAGGCAT CAGTAAACTG 
AAGAGCGACA TGAAGATGCT GGAGCGCCGG TTGTCTATGC TGGACCCCAG TAAGCTCGAC 
CAGCCTGTGA CGCCCACTGA GAAGGGTATC CGAGCTCAGT CAATGGACGT AGAAAGCATC 
GAATCTGGGA GGTACAGACT GTCCTCTGTA GAGTCTATTG ACGTTCCGGA CAGCTTCCCT 
GTTTCCAAGG ACACCAGACA TGGCCAGTGG CTGCGCAGGA GGCGGCTGGA CGGAGCGCTG 
AACAGGGTCC CCGTTGGCTT CTACCAGAAA GTGTGGAAGA TCCTGCAGAA GTGCCATGGT 
CTGTCCATAG AGGGCTTTGT CCTTCCATCT TCAACCACCA GAGAGATGAC ACCTGGAGAG 
ATTAAGTTCT CCGTCCACGT GGAGACGGTG CTGAACCGTG TCCCCCAGCC CGAGTACCGA 
CAGCTGCTGG TGGAGGCCAT CCTGGTCCTC ACCATGCTGG CCGACGTGGA CATCCCCAGC 
ATCGGCTCCA TCATTCACGT GGAAAAGATC GTCCACCAGG CCAACGACAT GTTCTACAAA 
GATCAGAAGG ACCTGGGAGC GGAAGAGCAC ATCCTGGAGA AGGACCCGTT CACGGGAGTG 
TGCAAGCTGT TGTACGACAG CGCTCCCAGT GGCCGCTTTG GGAGCATGAC CTACCTCACT 
AAGGCTGTGG CGGTGTATGT GCAGGACTTC CTGCCCAGCG GGGCCTGTGC GGTGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1 ( XM_023345749.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023201517.1 Xiphophorus maculatus phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X2, mRNA.

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CloneID	OXi23727
Clone ID Related Accession (Same CDS sequence)	XM_023345749.1
Accession Version	XM_023345749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036456.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGTC GGAGCAACTC TGGCGTTAAA TTGGACAACT ATGCCCGGAT AGTGCAGGAA 
ACTATCCTGC AGCACCAAGA TCCGGTGACG GGTCTTCTGC CAGGCAGCAC CGACCAGCCG 
GACGCCTGGG TCAGAGACAA TGTGTACAGC ATTGCGTCCG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAGA ACGCAGACAG AGACGAAGAC AAGGCCAAGG CCTATGAGCT GGAACAGAGC 
GTGGTGAAGT TGATGAGAGG TGTTCTCCAG TGTATAATGA GGCAGCTGGA CAAAGTGGAG 
AAGTTTAAAT ACAGCCGGAG CACTAAAGAC TCGCTCCACG CCAAGTACAA CACCAAGACC 
TGCGCCACCG TCGTCGGCGA CGACCAGTGG GGCCACCTGC AGGTGGACGC CACCTCTCTG 
TTCCTGCTCT TCCTCGCTCA GATGACTGCA TCAGGTCTCC ATATCATCTA CACCCAAGAC 
GAAGTGGATG TCATTCAGAA TCTCATGTTC TACATCGAGG CAGCGTACAA AGTGGCTGAC 
TATGGGATGT GGGAGAGAGG AGACAAAACC AACCAGGGCA TCACAGAGAT CAACGCCAGC 
TCTATAGGCA CGGCTAAGGC AGCTTTGGAG GCTCTGGATG AACTTAACCT GTTTGGAGCA 
AAAGGAGGGC CTGGATCTGT GGTCCATGCT CTGGCCGATG ACATACAGCA CTGTCAGTCT 
ATTCTGACGT CAATGTTACC CAGAGCCTCC ATCTCTAAAG AGGTAGACGC CGGAGTCCTG 
GCCATCATCA CCTACCCCGC CTTCGCTGTG GAGGACATGA GCATAGTGAA CATGACGAAG 
GAGGAGATCA TCTCTAAACT GCAGGGTCGA TACGGCTGCT GCAGGTTTCT CAGAGACGGC 
CACAGGACTC CCCGAGAGGA TCCAAACCGG CTGTATTACG AGTCCGCTGA GCTGAAGCTG 
TTTGAAAACA TAGAGTGCGA GTGGCCTCTG TTTTGGACGT ATCTCATACT CGACGGCATT 
TTTAGCAACA GCCATGAGCA GGTGCTGGAG TACCAGGAGG CGCTGGAGGC GATCCTGATC 
AAACAGAAAG ATGGGATCCG GCTGCTGCCA GAACTCTACA GTGTCCCTCC AGATAAGGTG 
GAAGAGGAGT ACATGAACCC TCACTCAGTA GAAAGGATCC CAATGGGGAA GTGTCCTCTA 
AAATGGGGTC AATCGTTGTA CATCCTTGGA AAACTCCTCT CTGAGGGTTT TCTCGCCCCC 
GGAGAGATCG ATCCCCTTAA CAGACGGTTC TCCACGATCC CTAAGCCTGA TGTAGTTGTA 
CAGGTGTCCA TTTTGGCTGA GACGGATGAG ATTAAGGGGC TGCTGATGAA GAATGGTATA 
AACGTGGAGA CCGTGGCGGA TATCCATCCC ATCCATGTTC AGCCCTCCAG AGTCCTGAGC 
CACATCTATG CCAGACTCGG CCGTAACCCA AGGCTCGGCC TTACAGGGAG ACCCTACAGA 
AGAATAGGAG TCCTGGGAAC CTCCAAGTTC TACATCATTA GAAACACAAT CTTTGCTTTC 
ACTCCTCAGT TCATCGACCA CCAGCAGTTC TACTTGGCTC TGGACAACAA GATGATCGTG 
GAGATGCTGA GGAACGAGAT TGCCTACCTT GCGTCCAGAT GGAGGATAAC CGGACGACCG 
ACGGTCACCT TCCCCATCTC ACAGACCATG CTGACTGAAG ACCGCACCGA CCTGGACCCT 
GCAGTCATAG CTATGCTAAA GAAGCTACAG GACGGATATT ACGGAGGAGC AAGGACCCAG 
ACGGGAAAGC TGTCGGAGTT CCTGACTACT TCCTGCTGCG CTCACCTCAG CTTCCTCGAC 
GGTAAGGGTT CCGGCAGCAT GGGCCGGCAC GGCGACCAGT ATGATGATGA TGATGACGGC 
GATGGATACA TGCATGAATT GCGTTGTGAT GATGAAGCGG ACGACCTCGC CCAGTATCTG 
GACCACCTGT TGGCGCACTC TTCCCCCAAG AAGTCTAAGC GTCAGCCAGG AGGCCTGGGC 
AGGTTCAAGG CGGCGGCCAC CAAGACCAAA GAGATGGTTT CTCTGATGAA CAAGGCTCAG 
GATCTGAACG TACACAACGT CAACATGTAC CTACCGAGCA AGCTGTTTCA TTCCTGTCAG 
CCGTCACTCA ACCTGAATCT CCCAGACCCT TCTGAGCCGC AGGAAACGGA GAGCTCGGCG 
GTGTCGGTCA CAGCGGAGAG CGGCATCCCC AGAGACGAAA GCGGAGCCAT CGACTACGAC 
GCTCTGGTGC AGCTGCTGAA GGACACGAAA AACCTCCAGG ACCAGGCAGA TATCCTTTAC 
ATCCTCTTCA AAGACAAGGG CATGGAGTGG GACACCCAGC TGCAAGGCAA AGGCTCCACA 
GTCAGATCTC TACTGAGCGA GCTTTATGAG AAGGCTGGTG ACCTCAAACA CTGGGGCCTC 
GTCAGGATGA TCTCTGGGAT GCTGAAGAAG AAGGTGGAGG AGCTCGACTC GGCTTGCTCT 
GACTTGCTGG CGCACCAGAA GCACCTGACC GTGGGACTTC CACCTGAGCC GAGAGAGAAG 
ACCATCACGG CTCCCATCCC TCCGGACCAG CTGGCCGCTC TCATCGACGA GGCCAGCGAC 
AACAACATCA GCTTGGCCAT CCTCACCCAG GAGATCATGG TGTACCTGGC GATGAGCATC 
AGGACTCAGC CGTCTCTGTT CAGCGAGATG TTCCGGCTGC GGATCGGCCT CATTATTCAG 
GTCATGGCTA CCGAACTGGC TCAGTCACTC AGCTGCTCAG GAGAGGAAGC CACAGAGAGC 
CTGATGAGTC TCAGCCCGTC TGAGCTGAAG AATCTGCTGC ACCACATCCT GAGTGGGAAA 
GAGTTCGGAG TGCAGCGCAG CGTAAGGGAG GCAGACGTCG GCGTCAGTCC TGCCATCTCC 
ATCCATCACC TAGGCAACAC CGGCGCCACC AAGAGCGAGA GAGCAGGCAT CAGTAAACTG 
AAGAGCGACA TGAAGATGCT GGAGCGCCGG TTGTCTATGC TGGACCCCAG TAAGGGTATC 
CGAGCTCAGT CAATGGACGT AGAAAGCATC GAATCTGGGA GGTACAGACT GTCCTCTGTA 
GAGTCTATTG ACGTTCCGGA CAGCTTCCCT GTTTCCAAGG ACACCAGACA TGGCCAGTGG 
CTGCGCAGGA GGCGGCTGGA CGGAGCGCTG AACAGGGTCC CCGTTGGCTT CTACCAGAAA 
GTGTGGAAGA TCCTGCAGAA GTGCCATGGT CTGTCCATAG AGGGCTTTGT CCTTCCATCT 
TCAACCACCA GAGAGATGAC ACCTGGAGAG ATTAAGTTCT CCGTCCACGT GGAGACGGTG 
CTGAACCGTG TCCCCCAGCC CGAGTACCGA CAGCTGCTGG TGGAGGCCAT CCTGGTCCTC 
ACCATGCTGG CCGACGTGGA CATCCCCAGC ATCGGCTCCA TCATTCACGT GGAAAAGATC 
GTCCACCAGG CCAACGACAT GTTCTACAAA GATCAGAAGG ACCTGGGAGC GGAAGAGCAC 
ATCCTGGAGA AGGACCCGTT CACGGGAGTG TGCAAGCTGT TGTACGACAG CGCTCCCAGT 
GGCCGCTTTG GGAGCATGAC CTACCTCACT AAGGCTGTGG CGGTGTATGT GCAGGACTTC 
CTGCCCAGCG GGGCCTGTGC GGTGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023201517.1
CDS	176..3865
Translation	MRSRSNSGVKLDNYARIVQETILQHQDPVTGLLPGSTDQPDAWVRDNVYSIASVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSRSTKDSLHAKYNTKTCATVVGDDQWGHLQVDATSLFLLFLAQMTASGLHIIYTQDEVDVIQNLMFYIEAAYKVADYGMWERGDKTNQGITEINASSIGTAKAALEALDELNLFGAKGGPGSVVHALADDIQHCQSILTSMLPRASISKEVDAGVLAIITYPAFAVEDMSIVNMTKEEIISKLQGRYGCCRFLRDGHRTPREDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFSNSHEQVLEYQEALEAILIKQKDGIRLLPELYSVPPDKVEEEYMNPHSVERIPMGKCPLKWGQSLYILGKLLSEGFLAPGEIDPLNRRFSTIPKPDVVVQVSILAETDEIKGLLMKNGINVETVADIHPIHVQPSRVLSHIYARLGRNPRLGLTGRPYRRIGVLGTSKFYIIRNTIFAFTPQFIDHQQFYLALDNKMIVEMLRNEIAYLASRWRITGRPTVTFPISQTMLTEDRTDLDPAVIAMLKKLQDGYYGGARTQTGKLSEFLTTSCCAHLSFLDGKGSGSMGRHGDQYDDDDDGDGYMHELRCDDEADDLAQYLDHLLAHSSPKKSKRQPGGLGRFKAAATKTKEMVSLMNKAQDLNVHNVNMYLPSKLFHSCQPSLNLNLPDPSEPQETESSAVSVTAESGIPRDESGAIDYDALVQLLKDTKNLQDQADILYILFKDKGMEWDTQLQGKGSTVRSLLSELYEKAGDLKHWGLVRMISGMLKKKVEELDSACSDLLAHQKHLTVGLPPEPREKTITAPIPPDQLAALIDEASDNNISLAILTQEIMVYLAMSIRTQPSLFSEMFRLRIGLIIQVMATELAQSLSCSGEEATESLMSLSPSELKNLLHHILSGKEFGVQRSVREADVGVSPAISIHHLGNTGATKSERAGISKLKSDMKMLERRLSMLDPSKGIRAQSMDVESIESGRYRLSSVESIDVPDSFPVSKDTRHGQWLRRRRLDGALNRVPVGFYQKVWKILQKCHGLSIEGFVLPSSTTREMTPGEIKFSVHVETVLNRVPQPEYRQLLVEAILVLTMLADVDIPSIGSIIHVEKIVHQANDMFYKDQKDLGAEEHILEKDPFTGVCKLLYDSAPSGRFGSMTYLTKAVAVYVQDFLPSGACAVQ

Target ORF information:

RefSeq Version	XM_023345749.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023345749.1

ORF Insert Sequence:

ATGAGAAGTC GGAGCAACTC TGGCGTTAAA TTGGACAACT ATGCCCGGAT AGTGCAGGAA 
ACTATCCTGC AGCACCAAGA TCCGGTGACG GGTCTTCTGC CAGGCAGCAC CGACCAGCCG 
GACGCCTGGG TCAGAGACAA TGTGTACAGC ATTGCGTCCG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAGA ACGCAGACAG AGACGAAGAC AAGGCCAAGG CCTATGAGCT GGAACAGAGC 
GTGGTGAAGT TGATGAGAGG TGTTCTCCAG TGTATAATGA GGCAGCTGGA CAAAGTGGAG 
AAGTTTAAAT ACAGCCGGAG CACTAAAGAC TCGCTCCACG CCAAGTACAA CACCAAGACC 
TGCGCCACCG TCGTCGGCGA CGACCAGTGG GGCCACCTGC AGGTGGACGC CACCTCTCTG 
TTCCTGCTCT TCCTCGCTCA GATGACTGCA TCAGGTCTCC ATATCATCTA CACCCAAGAC 
GAAGTGGATG TCATTCAGAA TCTCATGTTC TACATCGAGG CAGCGTACAA AGTGGCTGAC 
TATGGGATGT GGGAGAGAGG AGACAAAACC AACCAGGGCA TCACAGAGAT CAACGCCAGC 
TCTATAGGCA CGGCTAAGGC AGCTTTGGAG GCTCTGGATG AACTTAACCT GTTTGGAGCA 
AAAGGAGGGC CTGGATCTGT GGTCCATGCT CTGGCCGATG ACATACAGCA CTGTCAGTCT 
ATTCTGACGT CAATGTTACC CAGAGCCTCC ATCTCTAAAG AGGTAGACGC CGGAGTCCTG 
GCCATCATCA CCTACCCCGC CTTCGCTGTG GAGGACATGA GCATAGTGAA CATGACGAAG 
GAGGAGATCA TCTCTAAACT GCAGGGTCGA TACGGCTGCT GCAGGTTTCT CAGAGACGGC 
CACAGGACTC CCCGAGAGGA TCCAAACCGG CTGTATTACG AGTCCGCTGA GCTGAAGCTG 
TTTGAAAACA TAGAGTGCGA GTGGCCTCTG TTTTGGACGT ATCTCATACT CGACGGCATT 
TTTAGCAACA GCCATGAGCA GGTGCTGGAG TACCAGGAGG CGCTGGAGGC GATCCTGATC 
AAACAGAAAG ATGGGATCCG GCTGCTGCCA GAACTCTACA GTGTCCCTCC AGATAAGGTG 
GAAGAGGAGT ACATGAACCC TCACTCAGTA GAAAGGATCC CAATGGGGAA GTGTCCTCTA 
AAATGGGGTC AATCGTTGTA CATCCTTGGA AAACTCCTCT CTGAGGGTTT TCTCGCCCCC 
GGAGAGATCG ATCCCCTTAA CAGACGGTTC TCCACGATCC CTAAGCCTGA TGTAGTTGTA 
CAGGTGTCCA TTTTGGCTGA GACGGATGAG ATTAAGGGGC TGCTGATGAA GAATGGTATA 
AACGTGGAGA CCGTGGCGGA TATCCATCCC ATCCATGTTC AGCCCTCCAG AGTCCTGAGC 
CACATCTATG CCAGACTCGG CCGTAACCCA AGGCTCGGCC TTACAGGGAG ACCCTACAGA 
AGAATAGGAG TCCTGGGAAC CTCCAAGTTC TACATCATTA GAAACACAAT CTTTGCTTTC 
ACTCCTCAGT TCATCGACCA CCAGCAGTTC TACTTGGCTC TGGACAACAA GATGATCGTG 
GAGATGCTGA GGAACGAGAT TGCCTACCTT GCGTCCAGAT GGAGGATAAC CGGACGACCG 
ACGGTCACCT TCCCCATCTC ACAGACCATG CTGACTGAAG ACCGCACCGA CCTGGACCCT 
GCAGTCATAG CTATGCTAAA GAAGCTACAG GACGGATATT ACGGAGGAGC AAGGACCCAG 
ACGGGAAAGC TGTCGGAGTT CCTGACTACT TCCTGCTGCG CTCACCTCAG CTTCCTCGAC 
GGTAAGGGTT CCGGCAGCAT GGGCCGGCAC GGCGACCAGT ATGATGATGA TGATGACGGC 
GATGGATACA TGCATGAATT GCGTTGTGAT GATGAAGCGG ACGACCTCGC CCAGTATCTG 
GACCACCTGT TGGCGCACTC TTCCCCCAAG AAGTCTAAGC GTCAGCCAGG AGGCCTGGGC 
AGGTTCAAGG CGGCGGCCAC CAAGACCAAA GAGATGGTTT CTCTGATGAA CAAGGCTCAG 
GATCTGAACG TACACAACGT CAACATGTAC CTACCGAGCA AGCTGTTTCA TTCCTGTCAG 
CCGTCACTCA ACCTGAATCT CCCAGACCCT TCTGAGCCGC AGGAAACGGA GAGCTCGGCG 
GTGTCGGTCA CAGCGGAGAG CGGCATCCCC AGAGACGAAA GCGGAGCCAT CGACTACGAC 
GCTCTGGTGC AGCTGCTGAA GGACACGAAA AACCTCCAGG ACCAGGCAGA TATCCTTTAC 
ATCCTCTTCA AAGACAAGGG CATGGAGTGG GACACCCAGC TGCAAGGCAA AGGCTCCACA 
GTCAGATCTC TACTGAGCGA GCTTTATGAG AAGGCTGGTG ACCTCAAACA CTGGGGCCTC 
GTCAGGATGA TCTCTGGGAT GCTGAAGAAG AAGGTGGAGG AGCTCGACTC GGCTTGCTCT 
GACTTGCTGG CGCACCAGAA GCACCTGACC GTGGGACTTC CACCTGAGCC GAGAGAGAAG 
ACCATCACGG CTCCCATCCC TCCGGACCAG CTGGCCGCTC TCATCGACGA GGCCAGCGAC 
AACAACATCA GCTTGGCCAT CCTCACCCAG GAGATCATGG TGTACCTGGC GATGAGCATC 
AGGACTCAGC CGTCTCTGTT CAGCGAGATG TTCCGGCTGC GGATCGGCCT CATTATTCAG 
GTCATGGCTA CCGAACTGGC TCAGTCACTC AGCTGCTCAG GAGAGGAAGC CACAGAGAGC 
CTGATGAGTC TCAGCCCGTC TGAGCTGAAG AATCTGCTGC ACCACATCCT GAGTGGGAAA 
GAGTTCGGAG TGCAGCGCAG CGTAAGGGAG GCAGACGTCG GCGTCAGTCC TGCCATCTCC 
ATCCATCACC TAGGCAACAC CGGCGCCACC AAGAGCGAGA GAGCAGGCAT CAGTAAACTG 
AAGAGCGACA TGAAGATGCT GGAGCGCCGG TTGTCTATGC TGGACCCCAG TAAGGGTATC 
CGAGCTCAGT CAATGGACGT AGAAAGCATC GAATCTGGGA GGTACAGACT GTCCTCTGTA 
GAGTCTATTG ACGTTCCGGA CAGCTTCCCT GTTTCCAAGG ACACCAGACA TGGCCAGTGG 
CTGCGCAGGA GGCGGCTGGA CGGAGCGCTG AACAGGGTCC CCGTTGGCTT CTACCAGAAA 
GTGTGGAAGA TCCTGCAGAA GTGCCATGGT CTGTCCATAG AGGGCTTTGT CCTTCCATCT 
TCAACCACCA GAGAGATGAC ACCTGGAGAG ATTAAGTTCT CCGTCCACGT GGAGACGGTG 
CTGAACCGTG TCCCCCAGCC CGAGTACCGA CAGCTGCTGG TGGAGGCCAT CCTGGTCCTC 
ACCATGCTGG CCGACGTGGA CATCCCCAGC ATCGGCTCCA TCATTCACGT GGAAAAGATC 
GTCCACCAGG CCAACGACAT GTTCTACAAA GATCAGAAGG ACCTGGGAGC GGAAGAGCAC 
ATCCTGGAGA AGGACCCGTT CACGGGAGTG TGCAAGCTGT TGTACGACAG CGCTCCCAGT 
GGCCGCTTTG GGAGCATGAC CTACCTCACT AAGGCTGTGG CGGTGTATGT GCAGGACTTC 
CTGCCCAGCG GGGCCTGTGC GGTGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1 ( XM_023345750.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023201518.1 Xiphophorus maculatus phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXi23728
Clone ID Related Accession (Same CDS sequence)	XM_023345750.1
Accession Version	XM_023345750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036456.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGTC GGAGCAACTC TGGCGTTAAA TTGGACAACT ATGCCCGGAT AGTGCAGGAA 
ACTATCCTGC AGCACCAAGA TCCGGTGACG GGTCTTCTGC CAGGCAGCAC CGACCAGCCG 
GACGCCTGGG TCAGAGACAA TGTGTACAGC ATTGCGTCCG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAGA ACGCAGACAG AGACGAAGAC AAGGCCAAGG CCTATGAGCT GGAACAGAGC 
GTGGTGAAGT TGATGAGAGG TGTTCTCCAG TGTATAATGA GGCAGCTGGA CAAAGTGGAG 
AAGTTTAAAT ACAGCCGGAG CACTAAAGAC TCGCTCCACG CCAAGTACAA CACCAAGACC 
TGCGCCACCG TCGTCGGCGA CGACCAGTGG GGCCACCTGC AGGTGGACGC CACCTCTCTG 
TTCCTGCTCT TCCTCGCTCA GATGACTGCA TCAGGTCTCC ATATCATCTA CACCCAAGAC 
GAAGTGGATG TCATTCAGAA TCTCATGTTC TACATCGAGG CAGCGTACAA AGTGGCTGAC 
TATGGGATGT GGGAGAGAGG AGACAAAACC AACCAGGGCA TCACAGAGAT CAACGCCAGC 
TCTATAGGCA CGGCTAAGGC AGCTTTGGAG GCTCTGGATG AACTTAACCT GTTTGGAGCA 
AAAGGAGGGC CTGGATCTGT GGTCCATGCT CTGGCCGATG ACATACAGCA CTGTCAGTCT 
ATTCTGACGT CAATGTTACC CAGAGCCTCC ATCTCTAAAG AGGTAGACGC CGGAGTCCTG 
GCCATCATCA CCTACCCCGC CTTCGCTGTG GAGGACATGA GCATAGTGAA CATGACGAAG 
GAGGAGATCA TCTCTAAACT GCAGGGTCGA TACGGCTGCT GCAGGTTTCT CAGAGACGGC 
CACAGGACTC CCCGAGAGGA TCCAAACCGG CTGTATTACG AGTCCGCTGA GCTGAAGCTG 
TTTGAAAACA TAGAGTGCGA GTGGCCTCTG TTTTGGACGT ATCTCATACT CGACGGCATT 
TTTAGCAACA GCCATGAGCA GGTGCTGGAG TACCAGGAGG CGCTGGAGGC GATCCTGATC 
AAACAGAAAG ATGGGATCCG GCTGCTGCCA GAACTCTACA GTGTCCCTCC AGATAAGGTG 
GAAGAGGAGT ACATGAACCC TCACTCAGTA GAAAGGATCC CAATGGGGAA GTGTCCTCTA 
AAATGGGGTC AATCGTTGTA CATCCTTGGA AAACTCCTCT CTGAGGGTTT TCTCGCCCCC 
GGAGAGATCG ATCCCCTTAA CAGACGGTTC TCCACGATCC CTAAGCCTGA TGTAGTTGTA 
CAGGTGTCCA TTTTGGCTGA GACGGATGAG ATTAAGGGGC TGCTGATGAA GAATGGTATA 
AACGTGGAGA CCGTGGCGGA TATCCATCCC ATCCATGTTC AGCCCTCCAG AGTCCTGAGC 
CACATCTATG CCAGACTCGG CCGTAACCCA AGGCTCGGCC TTACAGGGAG ACCCTACAGA 
AGAATAGGAG TCCTGGGAAC CTCCAAGTTC TACATCATTA GAAACACAAT CTTTGCTTTC 
ACTCCTCAGT TCATCGACCA CCAGCAGTTC TACTTGGCTC TGGACAACAA GATGATCGTG 
GAGATGCTGA GGAACGAGAT TGCCTACCTT GCGTCCAGAT GGAGGATAAC CGGACGACCG 
ACGGTCACCT TCCCCATCTC ACAGACCATG CTGACTGAAG ACCGCACCGA CCTGGACCCT 
GCAGTCATAG CTATGCTAAA GAAGCTACAG GACGGATATT ACGGAGGAGC AAGGACCCAG 
ACGGGAAAGC TGTCGGAGTT CCTGACTACT TCCTGCTGCG CTCACCTCAG CTTCCTCGAC 
GGTAAGGGTT CCGGCAGCAT GGGCCGGCAC GGCGACCAGT ATGATGATGA TGATGACGGC 
GATGGATACA TGCATGAATT GCGTTGTGAT GATGAAGCGG ACGACCTCGC CCAGTATCTG 
GACCACCTGT TGGCGCACTC TTCCCCCAAG AAGTCTAAGC GTCAGCCAGG AGGCCTGGGC 
AGGTTCAAGG CGGCGGCCAC CAAGACCAAA GAGATGGTTT CTCTGATGAA CAAGGCTCAG 
GATCTGAACG TACACAACGT CAACATGTAC CTACCGAGCA AGCTGTTTCA TTCCTGTCAG 
CCGTCACTCA ACCTGAATCT CCCAGACCCT TCTGAGCCGC AGGAAACGGA GAGCTCGGCG 
GTGTCGGTCA CAGCGGAGAG CGGCATCCCC AGAGACGAAA GCGGAGCCAT CGACTACGAC 
GCTCTGGTGC AGCTGCTGAA GGACACGAAA AACCTCCAGG ACCAGGCAGA TATCCTTTAC 
ATCCTCTTCA AAGACAAGGG CATGGAGTGG GACACCCAGC TGCAAGGCAA AGGCTCCACA 
GTCAGATCTC TACTGAGCGA GCTTTATGAG AAGGCTGGTG ACCTCAAACA CTGGGGCCTC 
GTCAGGATGA TCTCTGGGAT GCTGAAGAAG AAGGTGGAGG AGCTCGACTC GGCTTGCTCT 
GACTTGCTGG CGCACCAGAA GCACCTGACC GTGGGACTTC CACCTGAGCC GAGAGAGAAG 
ACCATCACGG CTCCCATCCC TCCGGACCAG CTGGCCGCTC TCATCGACGA GGCCAGCGAC 
AACAACATCA GCTTGGCCAT CCTCACCCAG GAGATCATGG TGTACCTGGC GATGAGCATC 
AGGACTCAGC CGTCTCTGTT CAGCGAGATG TTCCGGCTGC GGATCGGCCT CATTATTCAG 
GTCATGGCTA CCGAACTGGC TCAGTCACTC AGCTGCTCAG GAGAGGAAGC CACAGAGAGC 
CTGATGAGTC TCAGCCCGTC TGAGCTGAAG AATCTGCTGC ACCACATCCT GAGTGGGAAA 
GAGTTCGGAG TGCAGCGCAG CGTAAGGGAG GCAGACGTCG GCGTCAGTCC TGCCATCTCC 
ATCCATCACC TAGGCAACAC CGGCGCCACC AAGAGCGAGA GAGCAGGCAT CAGTAAACTG 
AAGAGCGACA TGAAGATGGG TATCCGAGCT CAGTCAATGG ACGTAGAAAG CATCGAATCT 
GGGAGGTACA GACTGTCCTC TGTAGAGTCT ATTGACGTTC CGGACAGCTT CCCTGTTTCC 
AAGGACACCA GACATGGCCA GTGGCTGCGC AGGAGGCGGC TGGACGGAGC GCTGAACAGG 
GTCCCCGTTG GCTTCTACCA GAAAGTGTGG AAGATCCTGC AGAAGTGCCA TGGTCTGTCC 
ATAGAGGGCT TTGTCCTTCC ATCTTCAACC ACCAGAGAGA TGACACCTGG AGAGATTAAG 
TTCTCCGTCC ACGTGGAGAC GGTGCTGAAC CGTGTCCCCC AGCCCGAGTA CCGACAGCTG 
CTGGTGGAGG CCATCCTGGT CCTCACCATG CTGGCCGACG TGGACATCCC CAGCATCGGC 
TCCATCATTC ACGTGGAAAA GATCGTCCAC CAGGCCAACG ACATGTTCTA CAAAGATCAG 
AAGGACCTGG GAGCGGAAGA GCACATCCTG GAGAAGGACC CGTTCACGGG AGTGTGCAAG 
CTGTTGTACG ACAGCGCTCC CAGTGGCCGC TTTGGGAGCA TGACCTACCT CACTAAGGCT 
GTGGCGGTGT ATGTGCAGGA CTTCCTGCCC AGCGGGGCCT GTGCGGTGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023201518.1
CDS	176..3829
Translation	MRSRSNSGVKLDNYARIVQETILQHQDPVTGLLPGSTDQPDAWVRDNVYSIASVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSRSTKDSLHAKYNTKTCATVVGDDQWGHLQVDATSLFLLFLAQMTASGLHIIYTQDEVDVIQNLMFYIEAAYKVADYGMWERGDKTNQGITEINASSIGTAKAALEALDELNLFGAKGGPGSVVHALADDIQHCQSILTSMLPRASISKEVDAGVLAIITYPAFAVEDMSIVNMTKEEIISKLQGRYGCCRFLRDGHRTPREDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFSNSHEQVLEYQEALEAILIKQKDGIRLLPELYSVPPDKVEEEYMNPHSVERIPMGKCPLKWGQSLYILGKLLSEGFLAPGEIDPLNRRFSTIPKPDVVVQVSILAETDEIKGLLMKNGINVETVADIHPIHVQPSRVLSHIYARLGRNPRLGLTGRPYRRIGVLGTSKFYIIRNTIFAFTPQFIDHQQFYLALDNKMIVEMLRNEIAYLASRWRITGRPTVTFPISQTMLTEDRTDLDPAVIAMLKKLQDGYYGGARTQTGKLSEFLTTSCCAHLSFLDGKGSGSMGRHGDQYDDDDDGDGYMHELRCDDEADDLAQYLDHLLAHSSPKKSKRQPGGLGRFKAAATKTKEMVSLMNKAQDLNVHNVNMYLPSKLFHSCQPSLNLNLPDPSEPQETESSAVSVTAESGIPRDESGAIDYDALVQLLKDTKNLQDQADILYILFKDKGMEWDTQLQGKGSTVRSLLSELYEKAGDLKHWGLVRMISGMLKKKVEELDSACSDLLAHQKHLTVGLPPEPREKTITAPIPPDQLAALIDEASDNNISLAILTQEIMVYLAMSIRTQPSLFSEMFRLRIGLIIQVMATELAQSLSCSGEEATESLMSLSPSELKNLLHHILSGKEFGVQRSVREADVGVSPAISIHHLGNTGATKSERAGISKLKSDMKMGIRAQSMDVESIESGRYRLSSVESIDVPDSFPVSKDTRHGQWLRRRRLDGALNRVPVGFYQKVWKILQKCHGLSIEGFVLPSSTTREMTPGEIKFSVHVETVLNRVPQPEYRQLLVEAILVLTMLADVDIPSIGSIIHVEKIVHQANDMFYKDQKDLGAEEHILEKDPFTGVCKLLYDSAPSGRFGSMTYLTKAVAVYVQDFLPSGACAVQ

Target ORF information:

RefSeq Version	XM_023345750.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023345750.1

ORF Insert Sequence:

ATGAGAAGTC GGAGCAACTC TGGCGTTAAA TTGGACAACT ATGCCCGGAT AGTGCAGGAA 
ACTATCCTGC AGCACCAAGA TCCGGTGACG GGTCTTCTGC CAGGCAGCAC CGACCAGCCG 
GACGCCTGGG TCAGAGACAA TGTGTACAGC ATTGCGTCCG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAGA ACGCAGACAG AGACGAAGAC AAGGCCAAGG CCTATGAGCT GGAACAGAGC 
GTGGTGAAGT TGATGAGAGG TGTTCTCCAG TGTATAATGA GGCAGCTGGA CAAAGTGGAG 
AAGTTTAAAT ACAGCCGGAG CACTAAAGAC TCGCTCCACG CCAAGTACAA CACCAAGACC 
TGCGCCACCG TCGTCGGCGA CGACCAGTGG GGCCACCTGC AGGTGGACGC CACCTCTCTG 
TTCCTGCTCT TCCTCGCTCA GATGACTGCA TCAGGTCTCC ATATCATCTA CACCCAAGAC 
GAAGTGGATG TCATTCAGAA TCTCATGTTC TACATCGAGG CAGCGTACAA AGTGGCTGAC 
TATGGGATGT GGGAGAGAGG AGACAAAACC AACCAGGGCA TCACAGAGAT CAACGCCAGC 
TCTATAGGCA CGGCTAAGGC AGCTTTGGAG GCTCTGGATG AACTTAACCT GTTTGGAGCA 
AAAGGAGGGC CTGGATCTGT GGTCCATGCT CTGGCCGATG ACATACAGCA CTGTCAGTCT 
ATTCTGACGT CAATGTTACC CAGAGCCTCC ATCTCTAAAG AGGTAGACGC CGGAGTCCTG 
GCCATCATCA CCTACCCCGC CTTCGCTGTG GAGGACATGA GCATAGTGAA CATGACGAAG 
GAGGAGATCA TCTCTAAACT GCAGGGTCGA TACGGCTGCT GCAGGTTTCT CAGAGACGGC 
CACAGGACTC CCCGAGAGGA TCCAAACCGG CTGTATTACG AGTCCGCTGA GCTGAAGCTG 
TTTGAAAACA TAGAGTGCGA GTGGCCTCTG TTTTGGACGT ATCTCATACT CGACGGCATT 
TTTAGCAACA GCCATGAGCA GGTGCTGGAG TACCAGGAGG CGCTGGAGGC GATCCTGATC 
AAACAGAAAG ATGGGATCCG GCTGCTGCCA GAACTCTACA GTGTCCCTCC AGATAAGGTG 
GAAGAGGAGT ACATGAACCC TCACTCAGTA GAAAGGATCC CAATGGGGAA GTGTCCTCTA 
AAATGGGGTC AATCGTTGTA CATCCTTGGA AAACTCCTCT CTGAGGGTTT TCTCGCCCCC 
GGAGAGATCG ATCCCCTTAA CAGACGGTTC TCCACGATCC CTAAGCCTGA TGTAGTTGTA 
CAGGTGTCCA TTTTGGCTGA GACGGATGAG ATTAAGGGGC TGCTGATGAA GAATGGTATA 
AACGTGGAGA CCGTGGCGGA TATCCATCCC ATCCATGTTC AGCCCTCCAG AGTCCTGAGC 
CACATCTATG CCAGACTCGG CCGTAACCCA AGGCTCGGCC TTACAGGGAG ACCCTACAGA 
AGAATAGGAG TCCTGGGAAC CTCCAAGTTC TACATCATTA GAAACACAAT CTTTGCTTTC 
ACTCCTCAGT TCATCGACCA CCAGCAGTTC TACTTGGCTC TGGACAACAA GATGATCGTG 
GAGATGCTGA GGAACGAGAT TGCCTACCTT GCGTCCAGAT GGAGGATAAC CGGACGACCG 
ACGGTCACCT TCCCCATCTC ACAGACCATG CTGACTGAAG ACCGCACCGA CCTGGACCCT 
GCAGTCATAG CTATGCTAAA GAAGCTACAG GACGGATATT ACGGAGGAGC AAGGACCCAG 
ACGGGAAAGC TGTCGGAGTT CCTGACTACT TCCTGCTGCG CTCACCTCAG CTTCCTCGAC 
GGTAAGGGTT CCGGCAGCAT GGGCCGGCAC GGCGACCAGT ATGATGATGA TGATGACGGC 
GATGGATACA TGCATGAATT GCGTTGTGAT GATGAAGCGG ACGACCTCGC CCAGTATCTG 
GACCACCTGT TGGCGCACTC TTCCCCCAAG AAGTCTAAGC GTCAGCCAGG AGGCCTGGGC 
AGGTTCAAGG CGGCGGCCAC CAAGACCAAA GAGATGGTTT CTCTGATGAA CAAGGCTCAG 
GATCTGAACG TACACAACGT CAACATGTAC CTACCGAGCA AGCTGTTTCA TTCCTGTCAG 
CCGTCACTCA ACCTGAATCT CCCAGACCCT TCTGAGCCGC AGGAAACGGA GAGCTCGGCG 
GTGTCGGTCA CAGCGGAGAG CGGCATCCCC AGAGACGAAA GCGGAGCCAT CGACTACGAC 
GCTCTGGTGC AGCTGCTGAA GGACACGAAA AACCTCCAGG ACCAGGCAGA TATCCTTTAC 
ATCCTCTTCA AAGACAAGGG CATGGAGTGG GACACCCAGC TGCAAGGCAA AGGCTCCACA 
GTCAGATCTC TACTGAGCGA GCTTTATGAG AAGGCTGGTG ACCTCAAACA CTGGGGCCTC 
GTCAGGATGA TCTCTGGGAT GCTGAAGAAG AAGGTGGAGG AGCTCGACTC GGCTTGCTCT 
GACTTGCTGG CGCACCAGAA GCACCTGACC GTGGGACTTC CACCTGAGCC GAGAGAGAAG 
ACCATCACGG CTCCCATCCC TCCGGACCAG CTGGCCGCTC TCATCGACGA GGCCAGCGAC 
AACAACATCA GCTTGGCCAT CCTCACCCAG GAGATCATGG TGTACCTGGC GATGAGCATC 
AGGACTCAGC CGTCTCTGTT CAGCGAGATG TTCCGGCTGC GGATCGGCCT CATTATTCAG 
GTCATGGCTA CCGAACTGGC TCAGTCACTC AGCTGCTCAG GAGAGGAAGC CACAGAGAGC 
CTGATGAGTC TCAGCCCGTC TGAGCTGAAG AATCTGCTGC ACCACATCCT GAGTGGGAAA 
GAGTTCGGAG TGCAGCGCAG CGTAAGGGAG GCAGACGTCG GCGTCAGTCC TGCCATCTCC 
ATCCATCACC TAGGCAACAC CGGCGCCACC AAGAGCGAGA GAGCAGGCAT CAGTAAACTG 
AAGAGCGACA TGAAGATGGG TATCCGAGCT CAGTCAATGG ACGTAGAAAG CATCGAATCT 
GGGAGGTACA GACTGTCCTC TGTAGAGTCT ATTGACGTTC CGGACAGCTT CCCTGTTTCC 
AAGGACACCA GACATGGCCA GTGGCTGCGC AGGAGGCGGC TGGACGGAGC GCTGAACAGG 
GTCCCCGTTG GCTTCTACCA GAAAGTGTGG AAGATCCTGC AGAAGTGCCA TGGTCTGTCC 
ATAGAGGGCT TTGTCCTTCC ATCTTCAACC ACCAGAGAGA TGACACCTGG AGAGATTAAG 
TTCTCCGTCC ACGTGGAGAC GGTGCTGAAC CGTGTCCCCC AGCCCGAGTA CCGACAGCTG 
CTGGTGGAGG CCATCCTGGT CCTCACCATG CTGGCCGACG TGGACATCCC CAGCATCGGC 
TCCATCATTC ACGTGGAAAA GATCGTCCAC CAGGCCAACG ACATGTTCTA CAAAGATCAG 
AAGGACCTGG GAGCGGAAGA GCACATCCTG GAGAAGGACC CGTTCACGGG AGTGTGCAAG 
CTGTTGTACG ACAGCGCTCC CAGTGGCCGC TTTGGGAGCA TGACCTACCT CACTAAGGCT 
GTGGCGGTGT ATGTGCAGGA CTTCCTGCCC AGCGGGGCCT GTGCGGTGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1 ( XM_023345751.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023201519.1 Xiphophorus maculatus phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXi23729
Clone ID Related Accession (Same CDS sequence)	XM_023345751.1
Accession Version	XM_023345751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036456.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGTC GGAGCAACTC TGGCGTTAAA TTGGACAACT ATGCCCGGAT AGTGCAGGAA 
ACTATCCTGC AGCACCAAGA TCCGGTGACG GGTCTTCTGC CAGGCAGCAC CGACCAGCCG 
GACGCCTGGG TCAGAGACAA TGTGTACAGC ATTGCGTCCG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAGA ACGCAGACAG AGACGAAGAC AAGGCCAAGG CCTATGAGCT GGAACAGAGC 
GTGGTGAAGT TGATGAGAGG TGTTCTCCAG TGTATAATGA GGCAGCTGGA CAAAGTGGAG 
AAGTTTAAAT ACAGCCGGAG CACTAAAGAC TCGCTCCACG CCAAGTACAA CACCAAGACC 
TGCGCCACCG TCGTCGGCGA CGACCAGTGG GGCCACCTGC AGGTGGACGC CACCTCTCTG 
TTCCTGCTCT TCCTCGCTCA GATGACTGCA TCAGGTCTCC ATATCATCTA CACCCAAGAC 
GAAGTGGATG TCATTCAGAA TCTCATGTTC TACATCGAGG CAGCGTACAA AGTGGCTGAC 
TATGGGATGT GGGAGAGAGG AGACAAAACC AACCAGGGCA TCACAGAGAT CAACGCCAGC 
TCTATAGGCA CGGCTAAGGC AGCTTTGGAG GCTCTGGATG AACTTAACCT GTTTGGAGCA 
AAAGGAGGGC CTGGATCTGT GGTCCATGCT CTGGCCGATG ACATACAGCA CTGTCAGTCT 
ATTCTGACGT CAATGTTACC CAGAGCCTCC ATCTCTAAAG AGGTAGACGC CGGAGTCCTG 
GCCATCATCA CCTACCCCGC CTTCGCTGTG GAGGACATGA GCATAGTGAA CATGACGAAG 
GAGGAGATCA TCTCTAAACT GCAGGGTCGA TACGGCTGCT GCAGGTTTCT CAGAGACGGC 
CACAGGACTC CCCGAGAGGA TCCAAACCGG CTGTATTACG AGTCCGCTGA GCTGAAGCTG 
TTTGAAAACA TAGAGTGCGA GTGGCCTCTG TTTTGGACGT ATCTCATACT CGACGGCATT 
TTTAGCAACA GCCATGAGCA GGTGCTGGAG TACCAGGAGG CGCTGGAGGC GATCCTGATC 
AAACAGAAAG ATGGGATCCG GCTGCTGCCA GAACTCTACA GTGTCCCTCC AGATAAGGTG 
GAAGAGGAGT ACATGAACCC TCACTCAGTA GAAAGGATCC CAATGGGGAA GTGTCCTCTA 
AAATGGGGTC AATCGTTGTA CATCCTTGGA AAACTCCTCT CTGAGGGTTT TCTCGCCCCC 
GGAGAGATCG ATCCCCTTAA CAGACGGTTC TCCACGATCC CTAAGCCTGA TGTAGTTGTA 
CAGGTGTCCA TTTTGGCTGA GACGGATGAG ATTAAGGGGC TGCTGATGAA GAATGGTATA 
AACGTGGAGA CCGTGGCGGA TATCCATCCC ATCCATGTTC AGCCCTCCAG AGTCCTGAGC 
CACATCTATG CCAGACTCGG CCGTAACCCA AGGCTCGGCC TTACAGGGAG ACCCTACAGA 
AGAATAGGAG TCCTGGGAAC CTCCAAGTTC TACATCATTA GAAACACAAT CTTTGCTTTC 
ACTCCTCAGT TCATCGACCA CCAGCAGTTC TACTTGGCTC TGGACAACAA GATGATCGTG 
GAGATGCTGA GGAACGAGAT TGCCTACCTT GCGTCCAGAT GGAGGATAAC CGGACGACCG 
ACGGTCACCT TCCCCATCTC ACAGACCATG CTGACTGAAG ACCGCACCGA CCTGGACCCT 
GCAGTCATAG CTATGCTAAA GAAGCTACAG GACGGATATT ACGGAGGAGC AAGGACCCAG 
ACGGGAAAGC TGTCGGAGTT CCTGACTACT TCCTGCTGCG CTCACCTCAG CTTCCTCGAC 
GAAGCGGACG ACCTCGCCCA GTATCTGGAC CACCTGTTGG CGCACTCTTC CCCCAAGAAG 
TCTAAGCGTC AGCCAGGAGG CCTGGGCAGG TTCAAGGCGG CGGCCACCAA GACCAAAGAG 
ATGGTTTCTC TGATGAACAA GGCTCAGGAT CTGAACGTAC ACAACGTCAA CATGTACCTA 
CCGAGCAAGC TGTTTCATTC CTGTCAGCCG TCACTCAACC TGAATCTCCC AGACCCTTCT 
GAGCCGCAGG AAACGGAGAG CTCGGCGGTG TCGGTCACAG CGGAGAGCGG CATCCCCAGA 
GACGAAAGCG GAGCCATCGA CTACGACGCT CTGGTGCAGC TGCTGAAGGA CACGAAAAAC 
CTCCAGGACC AGGCAGATAT CCTTTACATC CTCTTCAAAG ACAAGGGCAT GGAGTGGGAC 
ACCCAGCTGC AAGGCAAAGG CTCCACAGTC AGATCTCTAC TGAGCGAGCT TTATGAGAAG 
GCTGGTGACC TCAAACACTG GGGCCTCGTC AGGATGATCT CTGGGATGCT GAAGAAGAAG 
GTGGAGGAGC TCGACTCGGC TTGCTCTGAC TTGCTGGCGC ACCAGAAGCA CCTGACCGTG 
GGACTTCCAC CTGAGCCGAG AGAGAAGACC ATCACGGCTC CCATCCCTCC GGACCAGCTG 
GCCGCTCTCA TCGACGAGGC CAGCGACAAC AACATCAGCT TGGCCATCCT CACCCAGGAG 
ATCATGGTGT ACCTGGCGAT GAGCATCAGG ACTCAGCCGT CTCTGTTCAG CGAGATGTTC 
CGGCTGCGGA TCGGCCTCAT TATTCAGGTC ATGGCTACCG AACTGGCTCA GTCACTCAGC 
TGCTCAGGAG AGGAAGCCAC AGAGAGCCTG ATGAGTCTCA GCCCGTCTGA GCTGAAGAAT 
CTGCTGCACC ACATCCTGAG TGGGAAAGAG TTCGGAGTGC AGCGCAGCGT AAGGGAGGCA 
GACGTCGGCG TCAGTCCTGC CATCTCCATC CATCACCTAG GCAACACCGG CGCCACCAAG 
AGCGAGAGAG CAGGCATCAG TAAACTGAAG AGCGACATGA AGATGCTGGA GCGCCGGTTG 
TCTATGCTGG ACCCCAGTAA GCTCGACCAG CCTGTGACGC CCACTGAGAA GGGTATCCGA 
GCTCAGTCAA TGGACGTAGA AAGCATCGAA TCTGGGAGGT ACAGACTGTC CTCTGTAGAG 
TCTATTGACG TTCCGGACAG CTTCCCTGTT TCCAAGGACA CCAGACATGG CCAGTGGCTG 
CGCAGGAGGC GGCTGGACGG AGCGCTGAAC AGGGTCCCCG TTGGCTTCTA CCAGAAAGTG 
TGGAAGATCC TGCAGAAGTG CCATGGTCTG TCCATAGAGG GCTTTGTCCT TCCATCTTCA 
ACCACCAGAG AGATGACACC TGGAGAGATT AAGTTCTCCG TCCACGTGGA GACGGTGCTG 
AACCGTGTCC CCCAGCCCGA GTACCGACAG CTGCTGGTGG AGGCCATCCT GGTCCTCACC 
ATGCTGGCCG ACGTGGACAT CCCCAGCATC GGCTCCATCA TTCACGTGGA AAAGATCGTC 
CACCAGGCCA ACGACATGTT CTACAAAGAT CAGAAGGACC TGGGAGCGGA AGAGCACATC 
CTGGAGAAGG ACCCGTTCAC GGGAGTGTGC AAGCTGTTGT ACGACAGCGC TCCCAGTGGC 
CGCTTTGGGA GCATGACCTA CCTCACTAAG GCTGTGGCGG TGTATGTGCA GGACTTCCTG 
CCCAGCGGGG CCTGTGCGGT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023201519.1
CDS	176..3802
Translation	MRSRSNSGVKLDNYARIVQETILQHQDPVTGLLPGSTDQPDAWVRDNVYSIASVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSRSTKDSLHAKYNTKTCATVVGDDQWGHLQVDATSLFLLFLAQMTASGLHIIYTQDEVDVIQNLMFYIEAAYKVADYGMWERGDKTNQGITEINASSIGTAKAALEALDELNLFGAKGGPGSVVHALADDIQHCQSILTSMLPRASISKEVDAGVLAIITYPAFAVEDMSIVNMTKEEIISKLQGRYGCCRFLRDGHRTPREDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFSNSHEQVLEYQEALEAILIKQKDGIRLLPELYSVPPDKVEEEYMNPHSVERIPMGKCPLKWGQSLYILGKLLSEGFLAPGEIDPLNRRFSTIPKPDVVVQVSILAETDEIKGLLMKNGINVETVADIHPIHVQPSRVLSHIYARLGRNPRLGLTGRPYRRIGVLGTSKFYIIRNTIFAFTPQFIDHQQFYLALDNKMIVEMLRNEIAYLASRWRITGRPTVTFPISQTMLTEDRTDLDPAVIAMLKKLQDGYYGGARTQTGKLSEFLTTSCCAHLSFLDEADDLAQYLDHLLAHSSPKKSKRQPGGLGRFKAAATKTKEMVSLMNKAQDLNVHNVNMYLPSKLFHSCQPSLNLNLPDPSEPQETESSAVSVTAESGIPRDESGAIDYDALVQLLKDTKNLQDQADILYILFKDKGMEWDTQLQGKGSTVRSLLSELYEKAGDLKHWGLVRMISGMLKKKVEELDSACSDLLAHQKHLTVGLPPEPREKTITAPIPPDQLAALIDEASDNNISLAILTQEIMVYLAMSIRTQPSLFSEMFRLRIGLIIQVMATELAQSLSCSGEEATESLMSLSPSELKNLLHHILSGKEFGVQRSVREADVGVSPAISIHHLGNTGATKSERAGISKLKSDMKMLERRLSMLDPSKLDQPVTPTEKGIRAQSMDVESIESGRYRLSSVESIDVPDSFPVSKDTRHGQWLRRRRLDGALNRVPVGFYQKVWKILQKCHGLSIEGFVLPSSTTREMTPGEIKFSVHVETVLNRVPQPEYRQLLVEAILVLTMLADVDIPSIGSIIHVEKIVHQANDMFYKDQKDLGAEEHILEKDPFTGVCKLLYDSAPSGRFGSMTYLTKAVAVYVQDFLPSGACAVQ

Target ORF information:

RefSeq Version	XM_023345751.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023345751.1

ORF Insert Sequence:

ATGAGAAGTC GGAGCAACTC TGGCGTTAAA TTGGACAACT ATGCCCGGAT AGTGCAGGAA 
ACTATCCTGC AGCACCAAGA TCCGGTGACG GGTCTTCTGC CAGGCAGCAC CGACCAGCCG 
GACGCCTGGG TCAGAGACAA TGTGTACAGC ATTGCGTCCG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAGA ACGCAGACAG AGACGAAGAC AAGGCCAAGG CCTATGAGCT GGAACAGAGC 
GTGGTGAAGT TGATGAGAGG TGTTCTCCAG TGTATAATGA GGCAGCTGGA CAAAGTGGAG 
AAGTTTAAAT ACAGCCGGAG CACTAAAGAC TCGCTCCACG CCAAGTACAA CACCAAGACC 
TGCGCCACCG TCGTCGGCGA CGACCAGTGG GGCCACCTGC AGGTGGACGC CACCTCTCTG 
TTCCTGCTCT TCCTCGCTCA GATGACTGCA TCAGGTCTCC ATATCATCTA CACCCAAGAC 
GAAGTGGATG TCATTCAGAA TCTCATGTTC TACATCGAGG CAGCGTACAA AGTGGCTGAC 
TATGGGATGT GGGAGAGAGG AGACAAAACC AACCAGGGCA TCACAGAGAT CAACGCCAGC 
TCTATAGGCA CGGCTAAGGC AGCTTTGGAG GCTCTGGATG AACTTAACCT GTTTGGAGCA 
AAAGGAGGGC CTGGATCTGT GGTCCATGCT CTGGCCGATG ACATACAGCA CTGTCAGTCT 
ATTCTGACGT CAATGTTACC CAGAGCCTCC ATCTCTAAAG AGGTAGACGC CGGAGTCCTG 
GCCATCATCA CCTACCCCGC CTTCGCTGTG GAGGACATGA GCATAGTGAA CATGACGAAG 
GAGGAGATCA TCTCTAAACT GCAGGGTCGA TACGGCTGCT GCAGGTTTCT CAGAGACGGC 
CACAGGACTC CCCGAGAGGA TCCAAACCGG CTGTATTACG AGTCCGCTGA GCTGAAGCTG 
TTTGAAAACA TAGAGTGCGA GTGGCCTCTG TTTTGGACGT ATCTCATACT CGACGGCATT 
TTTAGCAACA GCCATGAGCA GGTGCTGGAG TACCAGGAGG CGCTGGAGGC GATCCTGATC 
AAACAGAAAG ATGGGATCCG GCTGCTGCCA GAACTCTACA GTGTCCCTCC AGATAAGGTG 
GAAGAGGAGT ACATGAACCC TCACTCAGTA GAAAGGATCC CAATGGGGAA GTGTCCTCTA 
AAATGGGGTC AATCGTTGTA CATCCTTGGA AAACTCCTCT CTGAGGGTTT TCTCGCCCCC 
GGAGAGATCG ATCCCCTTAA CAGACGGTTC TCCACGATCC CTAAGCCTGA TGTAGTTGTA 
CAGGTGTCCA TTTTGGCTGA GACGGATGAG ATTAAGGGGC TGCTGATGAA GAATGGTATA 
AACGTGGAGA CCGTGGCGGA TATCCATCCC ATCCATGTTC AGCCCTCCAG AGTCCTGAGC 
CACATCTATG CCAGACTCGG CCGTAACCCA AGGCTCGGCC TTACAGGGAG ACCCTACAGA 
AGAATAGGAG TCCTGGGAAC CTCCAAGTTC TACATCATTA GAAACACAAT CTTTGCTTTC 
ACTCCTCAGT TCATCGACCA CCAGCAGTTC TACTTGGCTC TGGACAACAA GATGATCGTG 
GAGATGCTGA GGAACGAGAT TGCCTACCTT GCGTCCAGAT GGAGGATAAC CGGACGACCG 
ACGGTCACCT TCCCCATCTC ACAGACCATG CTGACTGAAG ACCGCACCGA CCTGGACCCT 
GCAGTCATAG CTATGCTAAA GAAGCTACAG GACGGATATT ACGGAGGAGC AAGGACCCAG 
ACGGGAAAGC TGTCGGAGTT CCTGACTACT TCCTGCTGCG CTCACCTCAG CTTCCTCGAC 
GAAGCGGACG ACCTCGCCCA GTATCTGGAC CACCTGTTGG CGCACTCTTC CCCCAAGAAG 
TCTAAGCGTC AGCCAGGAGG CCTGGGCAGG TTCAAGGCGG CGGCCACCAA GACCAAAGAG 
ATGGTTTCTC TGATGAACAA GGCTCAGGAT CTGAACGTAC ACAACGTCAA CATGTACCTA 
CCGAGCAAGC TGTTTCATTC CTGTCAGCCG TCACTCAACC TGAATCTCCC AGACCCTTCT 
GAGCCGCAGG AAACGGAGAG CTCGGCGGTG TCGGTCACAG CGGAGAGCGG CATCCCCAGA 
GACGAAAGCG GAGCCATCGA CTACGACGCT CTGGTGCAGC TGCTGAAGGA CACGAAAAAC 
CTCCAGGACC AGGCAGATAT CCTTTACATC CTCTTCAAAG ACAAGGGCAT GGAGTGGGAC 
ACCCAGCTGC AAGGCAAAGG CTCCACAGTC AGATCTCTAC TGAGCGAGCT TTATGAGAAG 
GCTGGTGACC TCAAACACTG GGGCCTCGTC AGGATGATCT CTGGGATGCT GAAGAAGAAG 
GTGGAGGAGC TCGACTCGGC TTGCTCTGAC TTGCTGGCGC ACCAGAAGCA CCTGACCGTG 
GGACTTCCAC CTGAGCCGAG AGAGAAGACC ATCACGGCTC CCATCCCTCC GGACCAGCTG 
GCCGCTCTCA TCGACGAGGC CAGCGACAAC AACATCAGCT TGGCCATCCT CACCCAGGAG 
ATCATGGTGT ACCTGGCGAT GAGCATCAGG ACTCAGCCGT CTCTGTTCAG CGAGATGTTC 
CGGCTGCGGA TCGGCCTCAT TATTCAGGTC ATGGCTACCG AACTGGCTCA GTCACTCAGC 
TGCTCAGGAG AGGAAGCCAC AGAGAGCCTG ATGAGTCTCA GCCCGTCTGA GCTGAAGAAT 
CTGCTGCACC ACATCCTGAG TGGGAAAGAG TTCGGAGTGC AGCGCAGCGT AAGGGAGGCA 
GACGTCGGCG TCAGTCCTGC CATCTCCATC CATCACCTAG GCAACACCGG CGCCACCAAG 
AGCGAGAGAG CAGGCATCAG TAAACTGAAG AGCGACATGA AGATGCTGGA GCGCCGGTTG 
TCTATGCTGG ACCCCAGTAA GCTCGACCAG CCTGTGACGC CCACTGAGAA GGGTATCCGA 
GCTCAGTCAA TGGACGTAGA AAGCATCGAA TCTGGGAGGT ACAGACTGTC CTCTGTAGAG 
TCTATTGACG TTCCGGACAG CTTCCCTGTT TCCAAGGACA CCAGACATGG CCAGTGGCTG 
CGCAGGAGGC GGCTGGACGG AGCGCTGAAC AGGGTCCCCG TTGGCTTCTA CCAGAAAGTG 
TGGAAGATCC TGCAGAAGTG CCATGGTCTG TCCATAGAGG GCTTTGTCCT TCCATCTTCA 
ACCACCAGAG AGATGACACC TGGAGAGATT AAGTTCTCCG TCCACGTGGA GACGGTGCTG 
AACCGTGTCC CCCAGCCCGA GTACCGACAG CTGCTGGTGG AGGCCATCCT GGTCCTCACC 
ATGCTGGCCG ACGTGGACAT CCCCAGCATC GGCTCCATCA TTCACGTGGA AAAGATCGTC 
CACCAGGCCA ACGACATGTT CTACAAAGAT CAGAAGGACC TGGGAGCGGA AGAGCACATC 
CTGGAGAAGG ACCCGTTCAC GGGAGTGTGC AAGCTGTTGT ACGACAGCGC TCCCAGTGGC 
CGCTTTGGGA GCATGACCTA CCTCACTAAG GCTGTGGCGG TGTATGTGCA GGACTTCCTG 
CCCAGCGGGG CCTGTGCGGT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka1 ( XM_023345752.1 ) cDNA ORF clone, Xiphophorus maculatus(southern platyfish) -> XP_023201520.1 Xiphophorus maculatus phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X5, mRNA.

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CloneID	OXi23730
Clone ID Related Accession (Same CDS sequence)	XM_023345752.1
Accession Version	XM_023345752.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036456.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGTC GGAGCAACTC TGGCGTTAAA TTGGACAACT ATGCCCGGAT AGTGCAGGAA 
ACTATCCTGC AGCACCAAGA TCCGGTGACG GGTCTTCTGC CAGGCAGCAC CGACCAGCCG 
GACGCCTGGG TCAGAGACAA TGTGTACAGC ATTGCGTCCG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAGA ACGCAGACAG AGACGAAGAC AAGGCCAAGG CCTATGAGCT GGAACAGAGC 
GTGGTGAAGT TGATGAGAGG TGTTCTCCAG TGTATAATGA GGCAGCTGGA CAAAGTGGAG 
AAGTTTAAAT ACAGCCGGAG CACTAAAGAC TCGCTCCACG CCAAGTACAA CACCAAGACC 
TGCGCCACCG TCGTCGGCGA CGACCAGTGG GGCCACCTGC AGGTGGACGC CACCTCTCTG 
TTCCTGCTCT TCCTCGCTCA GATGACTGCA TCAGGTCTCC ATATCATCTA CACCCAAGAC 
GAAGTGGATG TCATTCAGAA TCTCATGTTC TACATCGAGG CAGCGTACAA AGTGGCTGAC 
TATGGGATGT GGGAGAGAGG AGACAAAACC AACCAGGGCA TCACAGAGAT CAACGCCAGC 
TCTATAGGCA CGGCTAAGGC AGCTTTGGAG GCTCTGGATG AACTTAACCT GTTTGGAGCA 
AAAGGAGGGC CTGGATCTGT GGTCCATGCT CTGGCCGATG ACATACAGCA CTGTCAGTCT 
ATTCTGACGT CAATGTTACC CAGAGCCTCC ATCTCTAAAG AGGTAGACGC CGGAGTCCTG 
GCCATCATCA CCTACCCCGC CTTCGCTGTG GAGGACATGA GCATAGTGAA CATGACGAAG 
GAGGAGATCA TCTCTAAACT GCAGGGTCGA TACGGCTGCT GCAGGTTTCT CAGAGACGGC 
CACAGGACTC CCCGAGAGGA TCCAAACCGG CTGTATTACG AGTCCGCTGA GCTGAAGCTG 
TTTGAAAACA TAGAGTGCGA GTGGCCTCTG TTTTGGACGT ATCTCATACT CGACGGCATT 
TTTAGCAACA GCCATGAGCA GGTGCTGGAG TACCAGGAGG CGCTGGAGGC GATCCTGATC 
AAACAGAAAG ATGGGATCCG GCTGCTGCCA GAACTCTACA GTGTCCCTCC AGATAAGGTG 
GAAGAGGAGT ACATGAACCC TCACTCAGTA GAAAGGATCC CAATGGGGAA GTGTCCTCTA 
AAATGGGGTC AATCGTTGTA CATCCTTGGA AAACTCCTCT CTGAGGGTTT TCTCGCCCCC 
GGAGAGATCG ATCCCCTTAA CAGACGGTTC TCCACGATCC CTAAGCCTGA TGTAGTTGTA 
CAGGTGTCCA TTTTGGCTGA GACGGATGAG ATTAAGGGGC TGCTGATGAA GAATGGTATA 
AACGTGGAGA CCGTGGCGGA TATCCATCCC ATCCATGTTC AGCCCTCCAG AGTCCTGAGC 
CACATCTATG CCAGACTCGG CCGTAACCCA AGGCTCGGCC TTACAGGGAG ACCCTACAGA 
AGAATAGGAG TCCTGGGAAC CTCCAAGTTC TACATCATTA GAAACACAAT CTTTGCTTTC 
ACTCCTCAGT TCATCGACCA CCAGCAGTTC TACTTGGCTC TGGACAACAA GATGATCGTG 
GAGATGCTGA GGAACGAGAT TGCCTACCTT GCGTCCAGAT GGAGGATAAC CGGACGACCG 
ACGGTCACCT TCCCCATCTC ACAGACCATG CTGACTGAAG ACCGCACCGA CCTGGACCCT 
GCAGTCATAG CTATGCTAAA GAAGCTACAG GACGGATATT ACGGAGGAGC AAGGACCCAG 
ACGGGAAAGC TGTCGGAGTT CCTGACTACT TCCTGCTGCG CTCACCTCAG CTTCCTCGAC 
GGTAAGGGTT CCGGCAGCAT GGGCCGGCAC GGCGACCAGT ATGATGATGA TGATGACGGC 
GATGGATACA TGCATGAATT GCGTTGTGAT GATGAAGCGG ACGACCTCGC CCAGTATCTG 
GACCACCTGT TGGCGCACTC TTCCCCCAAG AAGTCTAAGC GTCAGCCAGG AGGCCTGGGC 
AGGTTCAAGG CGGCGGCCAC CAAGACCAAA GAGATGGTTT CTCTGATGAA CAAGGCTCAG 
GATCTGAACG TACACAACGT CAACATGTAC CTACCGAGCA AGCTGTTTCA TTCCTGTCAG 
CCGTCACTCA ACCTGAATCT CCCAGACCCT TCTGAGCCGC AGGAAACGGA GAGCTCGGCG 
GTGTCGGTCA CAGCGGAGAG CGGCATCCCC AGAGACGAAA GCGGAGCCAT CGACTACGAC 
GCTCTGGTGC AGCTGCTGAA GGACACGAAA AACCTCCAGG ACCAGGCAGA TATCCTTTAC 
ATCCTCTTCA AAGACAAGGG CATGGAGTGG GACACCCAGC TGCAAGGCAA AGGCTCCACA 
GTCAGATCTC TACTGAGCGA GCTTTATGAG AAGGCTGGTG ACCTCAAACA CTGGGGCCTC 
GTCAGGATGA TCTCTGGGAT GCTGAAGAAG AAGGTGGAGG AGCTCGACTC GGCTTGCTCT 
GACTTGCTGG CGCACCAGAA GCACCTGACC GTGGGACTTC CACCTGAGCC GAGAGAGAAG 
ACCATCACGG CTCCCATCCC TCCGGACCAG CTGGCCGCTC TCATCGACGA GGCCAGCGAC 
AACAACATCA GCTTGGCCAT CCTCACCCAG GAGATCATGG TGTACCTGGC GATGAGCATC 
AGGACTCAGC CGTCTCTGTT CAGCGAGATG TTCCGGCTGC GGATCGGCCT CATTATTCAG 
GTCATGGCTA CCGAACTGGC TCAGTCACTC AGCTGCTCAG GAGAGGAAGC CACAGAGAGC 
CTGATGAGTC TCAGCCCGTC TGAGCTGAAG AATCTGCTGC ACCACATCCT GAGTGGGAAA 
GAGTTCGGAG TGCAGCGCAG CGTAAGGGAG GCAGACGTCG GCGTCAGTCC TGCCATCTCC 
ATCCATCACC TAGGCAACAC CGGCGCCACC AAGAGCGAGA GAGCAGGCAT CAGTAAACTG 
AAGAGCGACA TGAAGATGAG GTACAGACTG TCCTCTGTAG AGTCTATTGA CGTTCCGGAC 
AGCTTCCCTG TTTCCAAGGA CACCAGACAT GGCCAGTGGC TGCGCAGGAG GCGGCTGGAC 
GGAGCGCTGA ACAGGGTCCC CGTTGGCTTC TACCAGAAAG TGTGGAAGAT CCTGCAGAAG 
TGCCATGGTC TGTCCATAGA GGGCTTTGTC CTTCCATCTT CAACCACCAG AGAGATGACA 
CCTGGAGAGA TTAAGTTCTC CGTCCACGTG GAGACGGTGC TGAACCGTGT CCCCCAGCCC 
GAGTACCGAC AGCTGCTGGT GGAGGCCATC CTGGTCCTCA CCATGCTGGC CGACGTGGAC 
ATCCCCAGCA TCGGCTCCAT CATTCACGTG GAAAAGATCG TCCACCAGGC CAACGACATG 
TTCTACAAAG ATCAGAAGGA CCTGGGAGCG GAAGAGCACA TCCTGGAGAA GGACCCGTTC 
ACGGGAGTGT GCAAGCTGTT GTACGACAGC GCTCCCAGTG GCCGCTTTGG GAGCATGACC 
TACCTCACTA AGGCTGTGGC GGTGTATGTG CAGGACTTCC TGCCCAGCGG GGCCTGTGCG 
GTGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023201520.1
CDS	176..3784
Translation	MRSRSNSGVKLDNYARIVQETILQHQDPVTGLLPGSTDQPDAWVRDNVYSIASVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSRSTKDSLHAKYNTKTCATVVGDDQWGHLQVDATSLFLLFLAQMTASGLHIIYTQDEVDVIQNLMFYIEAAYKVADYGMWERGDKTNQGITEINASSIGTAKAALEALDELNLFGAKGGPGSVVHALADDIQHCQSILTSMLPRASISKEVDAGVLAIITYPAFAVEDMSIVNMTKEEIISKLQGRYGCCRFLRDGHRTPREDPNRLYYESAELKLFENIECEWPLFWTYLILDGIFSNSHEQVLEYQEALEAILIKQKDGIRLLPELYSVPPDKVEEEYMNPHSVERIPMGKCPLKWGQSLYILGKLLSEGFLAPGEIDPLNRRFSTIPKPDVVVQVSILAETDEIKGLLMKNGINVETVADIHPIHVQPSRVLSHIYARLGRNPRLGLTGRPYRRIGVLGTSKFYIIRNTIFAFTPQFIDHQQFYLALDNKMIVEMLRNEIAYLASRWRITGRPTVTFPISQTMLTEDRTDLDPAVIAMLKKLQDGYYGGARTQTGKLSEFLTTSCCAHLSFLDGKGSGSMGRHGDQYDDDDDGDGYMHELRCDDEADDLAQYLDHLLAHSSPKKSKRQPGGLGRFKAAATKTKEMVSLMNKAQDLNVHNVNMYLPSKLFHSCQPSLNLNLPDPSEPQETESSAVSVTAESGIPRDESGAIDYDALVQLLKDTKNLQDQADILYILFKDKGMEWDTQLQGKGSTVRSLLSELYEKAGDLKHWGLVRMISGMLKKKVEELDSACSDLLAHQKHLTVGLPPEPREKTITAPIPPDQLAALIDEASDNNISLAILTQEIMVYLAMSIRTQPSLFSEMFRLRIGLIIQVMATELAQSLSCSGEEATESLMSLSPSELKNLLHHILSGKEFGVQRSVREADVGVSPAISIHHLGNTGATKSERAGISKLKSDMKMRYRLSSVESIDVPDSFPVSKDTRHGQWLRRRRLDGALNRVPVGFYQKVWKILQKCHGLSIEGFVLPSSTTREMTPGEIKFSVHVETVLNRVPQPEYRQLLVEAILVLTMLADVDIPSIGSIIHVEKIVHQANDMFYKDQKDLGAEEHILEKDPFTGVCKLLYDSAPSGRFGSMTYLTKAVAVYVQDFLPSGACAVQ

Target ORF information:

RefSeq Version	XM_023345752.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus phosphorylase kinase regulatory subunit alpha 1 (phka1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023345752.1

ORF Insert Sequence:

ATGAGAAGTC GGAGCAACTC TGGCGTTAAA TTGGACAACT ATGCCCGGAT AGTGCAGGAA 
ACTATCCTGC AGCACCAAGA TCCGGTGACG GGTCTTCTGC CAGGCAGCAC CGACCAGCCG 
GACGCCTGGG TCAGAGACAA TGTGTACAGC ATTGCGTCCG TGTGGGCCCT CAGCCTGGCC 
TACAGGAAGA ACGCAGACAG AGACGAAGAC AAGGCCAAGG CCTATGAGCT GGAACAGAGC 
GTGGTGAAGT TGATGAGAGG TGTTCTCCAG TGTATAATGA GGCAGCTGGA CAAAGTGGAG 
AAGTTTAAAT ACAGCCGGAG CACTAAAGAC TCGCTCCACG CCAAGTACAA CACCAAGACC 
TGCGCCACCG TCGTCGGCGA CGACCAGTGG GGCCACCTGC AGGTGGACGC CACCTCTCTG 
TTCCTGCTCT TCCTCGCTCA GATGACTGCA TCAGGTCTCC ATATCATCTA CACCCAAGAC 
GAAGTGGATG TCATTCAGAA TCTCATGTTC TACATCGAGG CAGCGTACAA AGTGGCTGAC 
TATGGGATGT GGGAGAGAGG AGACAAAACC AACCAGGGCA TCACAGAGAT CAACGCCAGC 
TCTATAGGCA CGGCTAAGGC AGCTTTGGAG GCTCTGGATG AACTTAACCT GTTTGGAGCA 
AAAGGAGGGC CTGGATCTGT GGTCCATGCT CTGGCCGATG ACATACAGCA CTGTCAGTCT 
ATTCTGACGT CAATGTTACC CAGAGCCTCC ATCTCTAAAG AGGTAGACGC CGGAGTCCTG 
GCCATCATCA CCTACCCCGC CTTCGCTGTG GAGGACATGA GCATAGTGAA CATGACGAAG 
GAGGAGATCA TCTCTAAACT GCAGGGTCGA TACGGCTGCT GCAGGTTTCT CAGAGACGGC 
CACAGGACTC CCCGAGAGGA TCCAAACCGG CTGTATTACG AGTCCGCTGA GCTGAAGCTG 
TTTGAAAACA TAGAGTGCGA GTGGCCTCTG TTTTGGACGT ATCTCATACT CGACGGCATT 
TTTAGCAACA GCCATGAGCA GGTGCTGGAG TACCAGGAGG CGCTGGAGGC GATCCTGATC 
AAACAGAAAG ATGGGATCCG GCTGCTGCCA GAACTCTACA GTGTCCCTCC AGATAAGGTG 
GAAGAGGAGT ACATGAACCC TCACTCAGTA GAAAGGATCC CAATGGGGAA GTGTCCTCTA 
AAATGGGGTC AATCGTTGTA CATCCTTGGA AAACTCCTCT CTGAGGGTTT TCTCGCCCCC 
GGAGAGATCG ATCCCCTTAA CAGACGGTTC TCCACGATCC CTAAGCCTGA TGTAGTTGTA 
CAGGTGTCCA TTTTGGCTGA GACGGATGAG ATTAAGGGGC TGCTGATGAA GAATGGTATA 
AACGTGGAGA CCGTGGCGGA TATCCATCCC ATCCATGTTC AGCCCTCCAG AGTCCTGAGC 
CACATCTATG CCAGACTCGG CCGTAACCCA AGGCTCGGCC TTACAGGGAG ACCCTACAGA 
AGAATAGGAG TCCTGGGAAC CTCCAAGTTC TACATCATTA GAAACACAAT CTTTGCTTTC 
ACTCCTCAGT TCATCGACCA CCAGCAGTTC TACTTGGCTC TGGACAACAA GATGATCGTG 
GAGATGCTGA GGAACGAGAT TGCCTACCTT GCGTCCAGAT GGAGGATAAC CGGACGACCG 
ACGGTCACCT TCCCCATCTC ACAGACCATG CTGACTGAAG ACCGCACCGA CCTGGACCCT 
GCAGTCATAG CTATGCTAAA GAAGCTACAG GACGGATATT ACGGAGGAGC AAGGACCCAG 
ACGGGAAAGC TGTCGGAGTT CCTGACTACT TCCTGCTGCG CTCACCTCAG CTTCCTCGAC 
GGTAAGGGTT CCGGCAGCAT GGGCCGGCAC GGCGACCAGT ATGATGATGA TGATGACGGC 
GATGGATACA TGCATGAATT GCGTTGTGAT GATGAAGCGG ACGACCTCGC CCAGTATCTG 
GACCACCTGT TGGCGCACTC TTCCCCCAAG AAGTCTAAGC GTCAGCCAGG AGGCCTGGGC 
AGGTTCAAGG CGGCGGCCAC CAAGACCAAA GAGATGGTTT CTCTGATGAA CAAGGCTCAG 
GATCTGAACG TACACAACGT CAACATGTAC CTACCGAGCA AGCTGTTTCA TTCCTGTCAG 
CCGTCACTCA ACCTGAATCT CCCAGACCCT TCTGAGCCGC AGGAAACGGA GAGCTCGGCG 
GTGTCGGTCA CAGCGGAGAG CGGCATCCCC AGAGACGAAA GCGGAGCCAT CGACTACGAC 
GCTCTGGTGC AGCTGCTGAA GGACACGAAA AACCTCCAGG ACCAGGCAGA TATCCTTTAC 
ATCCTCTTCA AAGACAAGGG CATGGAGTGG GACACCCAGC TGCAAGGCAA AGGCTCCACA 
GTCAGATCTC TACTGAGCGA GCTTTATGAG AAGGCTGGTG ACCTCAAACA CTGGGGCCTC 
GTCAGGATGA TCTCTGGGAT GCTGAAGAAG AAGGTGGAGG AGCTCGACTC GGCTTGCTCT 
GACTTGCTGG CGCACCAGAA GCACCTGACC GTGGGACTTC CACCTGAGCC GAGAGAGAAG 
ACCATCACGG CTCCCATCCC TCCGGACCAG CTGGCCGCTC TCATCGACGA GGCCAGCGAC 
AACAACATCA GCTTGGCCAT CCTCACCCAG GAGATCATGG TGTACCTGGC GATGAGCATC 
AGGACTCAGC CGTCTCTGTT CAGCGAGATG TTCCGGCTGC GGATCGGCCT CATTATTCAG 
GTCATGGCTA CCGAACTGGC TCAGTCACTC AGCTGCTCAG GAGAGGAAGC CACAGAGAGC 
CTGATGAGTC TCAGCCCGTC TGAGCTGAAG AATCTGCTGC ACCACATCCT GAGTGGGAAA 
GAGTTCGGAG TGCAGCGCAG CGTAAGGGAG GCAGACGTCG GCGTCAGTCC TGCCATCTCC 
ATCCATCACC TAGGCAACAC CGGCGCCACC AAGAGCGAGA GAGCAGGCAT CAGTAAACTG 
AAGAGCGACA TGAAGATGAG GTACAGACTG TCCTCTGTAG AGTCTATTGA CGTTCCGGAC 
AGCTTCCCTG TTTCCAAGGA CACCAGACAT GGCCAGTGGC TGCGCAGGAG GCGGCTGGAC 
GGAGCGCTGA ACAGGGTCCC CGTTGGCTTC TACCAGAAAG TGTGGAAGAT CCTGCAGAAG 
TGCCATGGTC TGTCCATAGA GGGCTTTGTC CTTCCATCTT CAACCACCAG AGAGATGACA 
CCTGGAGAGA TTAAGTTCTC CGTCCACGTG GAGACGGTGC TGAACCGTGT CCCCCAGCCC 
GAGTACCGAC AGCTGCTGGT GGAGGCCATC CTGGTCCTCA CCATGCTGGC CGACGTGGAC 
ATCCCCAGCA TCGGCTCCAT CATTCACGTG GAAAAGATCG TCCACCAGGC CAACGACATG 
TTCTACAAAG ATCAGAAGGA CCTGGGAGCG GAAGAGCACA TCCTGGAGAA GGACCCGTTC 
ACGGGAGTGT GCAAGCTGTT GTACGACAGC GCTCCCAGTG GCCGCTTTGG GAGCATGACC 
TACCTCACTA AGGCTGTGGC GGTGTATGTG CAGGACTTCC TGCCCAGCGG GGCCTGTGCG 
GTGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

phka1 cDNA ORF clone, Xiphophorus maculatus(southern platyfish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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