Tc00.1047053508479.120 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

Gene
Clones
gRNAs

Summary

Gene Symbol	Tc00.1047053508479.120
Entrez Gene ID	3553667
Full Name	pumilio/PUF RNA binding protein 8
Gene Type	protein-coding
Organism	Trypanosoma cruzi strain CL Brener

mRNA and Protein(s)

mRNA	Protein	Name
XM_815755.1	XP_820848.1	pumilio/PUF RNA binding protein 8

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Worthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K, Englund PT, Fazelina G, Feldblyum T, Ferella M, Frasch AC, Gull K, Horn D, Hou L, Huang Y, Kindlund E, Klingbeil M, Kluge S, Koo H, Lacerda D, Levin MJ, Lorenzi H, Louie T, Machado CR, McCulloch R, McKenna A, Mizuno Y, Mottram JC, Nelson S, Ochaya S, Osoegawa K, Pai G, Parsons M, Pentony M, Pettersson U, Pop M, Ramirez JL, Rinta J, Robertson L, Salzberg SL, Sanchez DO, Seyler A, Sharma R, Shetty J, Simpson AJ, Sisk E, Tammi MT, Tarleton R, Teixeira S, Van Aken S, Vogt C, Ward PN, Wickstead B, Wortman J, White O, Fraser CM, Stuart KD, Andersson B
Science (New York, N.Y.)309(5733)409-15(2005 Jul)

Comparative genomics of trypanosomatid parasitic protozoa.
El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran AN, Wortman JR, Alsmark UC, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N
Science (New York, N.Y.)309(5733)404-9(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tc00.1047053508479.120 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tc00.1047053508479.120 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTb15058	XM_815755.1 Latest version!	Trypanosoma cruzi strain CL Brener pumilio/PUF RNA binding protein 8 partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tc00.1047053508479.120 ( XM_815755.1 ) cDNA ORF clone, Trypanosoma cruzi strain CL Brener -> XP_820848.1 Trypanosoma cruzi strain CL Brener pumilio/PUF RNA binding protein 8 partial mRNA.

Price & Availability↑

CloneID	OTb15058
Clone ID Related Accession (Same CDS sequence)	XM_815755.1
Accession Version	XM_815755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1225382400000
Organism	Trypanosoma cruzi strain CL Brener
Product	pumilio/PUF RNA binding protein 8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001849463). COMPLETENESS: incomplete on both ends.

ATGGTCCAAA CGCACACAAA GAGGGAGGAG GCACGTGCCG AGAGGTTGCG GAAACTCCCA 
CAGACAGAGA GACTGAGAAA GAGTGCCCTG ATAAAGAAGC GGGAGGGACG AGTTCTCAGT 
GCGGCAGAAA AACACGCCAT GAAGATGACC TCCGAGTACG GGGAGATCAT TCGTCTGTGG 
GAGGTGCTGC GTGTGTCGTC CGATCCTGCG GCGAAGGACG CGGCGGCAAA AAGTGTGGGG 
CAGCGGCGGG CTGAGCGGTA CGAGCACAAG TACCCAACGG TGGATCTTCT GCTTAAACTC 
ATCGAGCCAA ATTTTGCGAC GTACGTGAAA ACACCACGTG TTAGTCGTGT CATACAGTCA 
ATGATTAAGT ACGCATCTTT GACCCAGTTG GAACGCATTC TTGTGCTTAT TTCAAATCCT 
TTTGTAACTC ACGCTACCGA CGCATACGGA CATTTTGTTG TAACCGCATT GATTCGCCAC 
GCACCGCATA GCTTTCTGGA CAAGTTACTT GCACTCATTA TTCCTGCAGT GCCTTCTTTG 
GTGTCGCATC GATTCGGCAT TGAAGTTGTG AATGCAGCCT ACAGCAGCCG TCTCTGCACA 
TCCGCCAATC GTCATATTCT TATTCTAGCC GTTCTGAAAG ACAAAATTGC CGTAATGAAG 
CGTTGGAAAG GATATCCCAT CTTAGAGGAG GTGCTGCAGC AAGAAGTGAC ACAACGGAAG 
CGTCTGCTTT CTAGACTTTT TGGGTTATGC GACACGCTTG TTTCGCAGAA ATGTGCTATC 
GGCTATCCGT TTGTTCAGCG CCTGGTTGCA GCGTACCTCA AACAGGGGGT AAAGGATGAT 
GTGATGGAAT TATGTGATAC TCTTCGGCCC CACTTGGCGG CATTGTGTGC CACCCGCGAG 
GGAGCTCCGA TTGTGACGAC GGTCTTCTTT CTGATCGAAC CTAAAAAAAG CAAAGAAGTG 
CTGCGCGCCC TCTCTGAGAA CCTTGGCAGC CTTTTGGTTG ACAAGTACGC CGCGCCAATT 
GTCGCACGCC TTTTTGATCT TGTCTATGAC GTGCAACTGT TACGCAAGTA TGTTGTTAAT 
GAATTGGAGG CACACCTCGC AGAGGTTATC AACAGCCCCT TTGGACACCA GATTGTGTTG 
CACCTTCTCA CGCCCCATGA GGAACGGAAG CAGAAATTTC TTCTTCCCAA CTGGTTCCAA 
CACAATCTAT TTTCTATGGA GAACAATGGG TGGAATCATC ACACATGGCT TACACATGAG 
TACGAGGAAG AGACGGTTGA AATACGCTCC AAGGGTGCCA TCAGTACGCA TCTGGTGGTG 
TTGCCTTCAA TTGTGAGAAA ATTTATTGAA ATTGCCAGCG ATCCCGATAA AGGGCTACGG 
ATAAATAAAC ACTACGTTGG CCTCATTGCA AGAGAGGTGC TGGTTGTAAA TGAGACGGAG 
GATGCCTACA GGGCGGCACT CCAGTTATCA GAGGAGGAAA TAAAAGTACT TACACGCCTG 
GCGCCTTCAA GGGATCCGAG TAAGCGGTTG CGTGAGGAGG ATAGCGGCTG TATGACAGGT 
CCAACGTCAA ATGGAAGTAA TGTCAAGGCT CATAAGGCTG ATACATCTTC TGGTTTGTTA 
AAAAAGCTCG CGATTTCTCA TGTGGCGCAG GAATCTCACA GTGCCGCATT ACATGCCGAT 
AAGAAGGCAA AGGTTGTGGC GAAGGGCAAA AAATTGGGAA AAGGTGCGAC GAAGACAACA 
CGCAGAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_820848.1
CDS	1..1752
Translation	MVQTHTKREEARAERLRKLPQTERLRKSALIKKREGRVLSAAEKHAMKMTSEYGEIIRLWEVLRVSSDPAAKDAAAKSVGQRRAERYEHKYPTVDLLLKLIEPNFATYVKTPRVSRVIQSMIKYASLTQLERILVLISNPFVTHATDAYGHFVVTALIRHAPHSFLDKLLALIIPAVPSLVSHRFGIEVVNAAYSSRLCTSANRHILILAVLKDKIAVMKRWKGYPILEEVLQQEVTQRKRLLSRLFGLCDTLVSQKCAIGYPFVQRLVAAYLKQGVKDDVMELCDTLRPHLAALCATREGAPIVTTVFFLIEPKKSKEVLRALSENLGSLLVDKYAAPIVARLFDLVYDVQLLRKYVVNELEAHLAEVINSPFGHQIVLHLLTPHEERKQKFLLPNWFQHNLFSMENNGWNHHTWLTHEYEEETVEIRSKGAISTHLVVLPSIVRKFIEIASDPDKGLRINKHYVGLIAREVLVVNETEDAYRAALQLSEEEIKVLTRLAPSRDPSKRLREEDSGCMTGPTSNGSNVKAHKADTSSGLLKKLAISHVAQESHSAALHADKKAKVVAKGKKLGKGATKTTRRK

Target ORF information:

RefSeq Version	XM_815755.1
Organism	Trypanosoma cruzi strain CL Brener
Definition	Trypanosoma cruzi strain CL Brener pumilio/PUF RNA binding protein 8 partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_815755.1

ORF Insert Sequence:

ATGGTCCAAA CGCACACAAA GAGGGAGGAG GCACGTGCCG AGAGGTTGCG GAAACTCCCA 
CAGACAGAGA GACTGAGAAA GAGTGCCCTG ATAAAGAAGC GGGAGGGACG AGTTCTCAGT 
GCGGCAGAAA AACACGCCAT GAAGATGACC TCCGAGTACG GGGAGATCAT TCGTCTGTGG 
GAGGTGCTGC GTGTGTCGTC CGATCCTGCG GCGAAGGACG CGGCGGCAAA AAGTGTGGGG 
CAGCGGCGGG CTGAGCGGTA CGAGCACAAG TACCCAACGG TGGATCTTCT GCTTAAACTC 
ATCGAGCCAA ATTTTGCGAC GTACGTGAAA ACACCACGTG TTAGTCGTGT CATACAGTCA 
ATGATTAAGT ACGCATCTTT GACCCAGTTG GAACGCATTC TTGTGCTTAT TTCAAATCCT 
TTTGTAACTC ACGCTACCGA CGCATACGGA CATTTTGTTG TAACCGCATT GATTCGCCAC 
GCACCGCATA GCTTTCTGGA CAAGTTACTT GCACTCATTA TTCCTGCAGT GCCTTCTTTG 
GTGTCGCATC GATTCGGCAT TGAAGTTGTG AATGCAGCCT ACAGCAGCCG TCTCTGCACA 
TCCGCCAATC GTCATATTCT TATTCTAGCC GTTCTGAAAG ACAAAATTGC CGTAATGAAG 
CGTTGGAAAG GATATCCCAT CTTAGAGGAG GTGCTGCAGC AAGAAGTGAC ACAACGGAAG 
CGTCTGCTTT CTAGACTTTT TGGGTTATGC GACACGCTTG TTTCGCAGAA ATGTGCTATC 
GGCTATCCGT TTGTTCAGCG CCTGGTTGCA GCGTACCTCA AACAGGGGGT AAAGGATGAT 
GTGATGGAAT TATGTGATAC TCTTCGGCCC CACTTGGCGG CATTGTGTGC CACCCGCGAG 
GGAGCTCCGA TTGTGACGAC GGTCTTCTTT CTGATCGAAC CTAAAAAAAG CAAAGAAGTG 
CTGCGCGCCC TCTCTGAGAA CCTTGGCAGC CTTTTGGTTG ACAAGTACGC CGCGCCAATT 
GTCGCACGCC TTTTTGATCT TGTCTATGAC GTGCAACTGT TACGCAAGTA TGTTGTTAAT 
GAATTGGAGG CACACCTCGC AGAGGTTATC AACAGCCCCT TTGGACACCA GATTGTGTTG 
CACCTTCTCA CGCCCCATGA GGAACGGAAG CAGAAATTTC TTCTTCCCAA CTGGTTCCAA 
CACAATCTAT TTTCTATGGA GAACAATGGG TGGAATCATC ACACATGGCT TACACATGAG 
TACGAGGAAG AGACGGTTGA AATACGCTCC AAGGGTGCCA TCAGTACGCA TCTGGTGGTG 
TTGCCTTCAA TTGTGAGAAA ATTTATTGAA ATTGCCAGCG ATCCCGATAA AGGGCTACGG 
ATAAATAAAC ACTACGTTGG CCTCATTGCA AGAGAGGTGC TGGTTGTAAA TGAGACGGAG 
GATGCCTACA GGGCGGCACT CCAGTTATCA GAGGAGGAAA TAAAAGTACT TACACGCCTG 
GCGCCTTCAA GGGATCCGAG TAAGCGGTTG CGTGAGGAGG ATAGCGGCTG TATGACAGGT 
CCAACGTCAA ATGGAAGTAA TGTCAAGGCT CATAAGGCTG ATACATCTTC TGGTTTGTTA 
AAAAAGCTCG CGATTTCTCA TGTGGCGCAG GAATCTCACA GTGCCGCATT ACATGCCGAT 
AAGAAGGCAA AGGTTGTGGC GAAGGGCAAA AAATTGGGAA AAGGTGCGAC GAAGACAACA 
CGCAGAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
Science (New York, N.Y.)309(5733)409-15(2005 Jul)
El-Sayed NM,Myler PJ,Bartholomeu DC,Nilsson D,Aggarwal G,Tran AN,Ghedin E,Worthey EA,Delcher AL,Blandin G,Westenberger SJ,Caler E,Cerqueira GC,Branche C,Haas B,Anupama A,Arner E,Aslund L,Attipoe P,Bontempi E,Bringaud F,Burton P,Cadag E,Campbell DA,Carrington M,Crabtree J,Darban H,da Silveira JF,de Jong P,Edwards K,Englund PT,Fazelina G,Feldblyum T,Ferella M,Frasch AC,Gull K,Horn D,Hou L,Huang Y,Kindlund E,Klingbeil M,Kluge S,Koo H,Lacerda D,Levin MJ,Lorenzi H,Louie T,Machado CR,McCulloch R,McKenna A,Mizuno Y,Mottram JC,Nelson S,Ochaya S,Osoegawa K,Pai G,Parsons M,Pentony M,Pettersson U,Pop M,Ramirez JL,Rinta J,Robertson L,Salzberg SL,Sanchez DO,Seyler A,Sharma R,Shetty J,Simpson AJ,Sisk E,Tammi MT,Tarleton R,Teixeira S,Van Aken S,Vogt C,Ward PN,Wickstead B,Wortman J,White O,Fraser CM,Stuart KD,Andersson B

Comparative genomics of trypanosomatid parasitic protozoa.
Science (New York, N.Y.)309(5733)404-9(2005 Jul)
El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N

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Tc00.1047053508479.120 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

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Target ORF information:

ORF Insert Sequence:

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