Tc00.1047053506147.180 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

Gene
Clones
gRNAs

Summary

Gene Symbol	Tc00.1047053506147.180
Entrez Gene ID	3549641
Full Name	DNA polymerase epsilon catalytic subunit
Gene Type	protein-coding
Organism	Trypanosoma cruzi strain CL Brener

mRNA and Protein(s)

mRNA	Protein	Name
XM_812510.1	XP_817603.1	DNA polymerase epsilon catalytic subunit

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Worthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K, Englund PT, Fazelina G, Feldblyum T, Ferella M, Frasch AC, Gull K, Horn D, Hou L, Huang Y, Kindlund E, Klingbeil M, Kluge S, Koo H, Lacerda D, Levin MJ, Lorenzi H, Louie T, Machado CR, McCulloch R, McKenna A, Mizuno Y, Mottram JC, Nelson S, Ochaya S, Osoegawa K, Pai G, Parsons M, Pentony M, Pettersson U, Pop M, Ramirez JL, Rinta J, Robertson L, Salzberg SL, Sanchez DO, Seyler A, Sharma R, Shetty J, Simpson AJ, Sisk E, Tammi MT, Tarleton R, Teixeira S, Van Aken S, Vogt C, Ward PN, Wickstead B, Wortman J, White O, Fraser CM, Stuart KD, Andersson B
Science (New York, N.Y.)309(5733)409-15(2005 Jul)

Comparative genomics of trypanosomatid parasitic protozoa.
El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran AN, Wortman JR, Alsmark UC, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N
Science (New York, N.Y.)309(5733)404-9(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tc00.1047053506147.180 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tc00.1047053506147.180 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTb12002	XM_812510.1 Latest version!	Trypanosoma cruzi strain CL Brener DNA polymerase epsilon catalytic subunit partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tc00.1047053506147.180 ( XM_812510.1 ) cDNA ORF clone, Trypanosoma cruzi strain CL Brener -> XP_817603.1 Trypanosoma cruzi strain CL Brener DNA polymerase epsilon catalytic subunit partial mRNA.

Price & Availability↑

CloneID	OTb12002
Clone ID Related Accession (Same CDS sequence)	XM_812510.1
Accession Version	XM_812510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1225382400000
Organism	Trypanosoma cruzi strain CL Brener
Product	DNA polymerase epsilon catalytic subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001849384). COMPLETENESS: incomplete on both ends.

ATGCCAGTTG CGCCCAATGA ATGGAAGGGA ATGGAGAGCG GCAGCAGGAC CGGCTGGCTA 
TTCAATTACC ACCCAACGAC GGTGATGGTG GCTGACGAGT CGTCAGTGGA GCGCCAAGGT 
GGATCGGGTG GGCGGGCCGC ACTTGTTCTT CTTCTGCAGG ATCATGACGG CGAAATATTT 
TTTGTGAATC TGTTCTATCG CCCCTACATT TTCGTCTCAG TGGTGGAAGG GCATGAACAC 
GAGGTGGAGC TTGGTCTCAT GTCTCTTTTT GGCCCGCAGC TGATTTACAA CGTTGAAACG 
GTCGAAAAGG AGGATTTAGA CCTTGTCAAC CACTTAAGCG GTCGGCGGCG GCTCTATCTG 
AAGGTTGTTT TTAGAAACGT GCAGGACTTA ACGGTGGTGC GTGGTCGACT TGAGAAATTT 
GTGAAGCGCA ACGCCTCCAT GGGGGCTACA AACCCGTTGC TAAACCCTTT TGAAGGATCA 
CTGAGAGATG TGATGGCCAA AACATTTGAG GATGACCCGT TCGATTCAAA CATTTCATCG 
GAGCGATGGG TTGACTGGGT GCAGGAGATG AGGGAATACG ACGTGAAGTA TCACATGCGG 
GTGGCCATTG ATTTGGGCAT TTTTGTTGGT GTGTGGTACG ATGTCATTGT GCATGAAGGC 
GAGGCGAAAG TGACACGCTG TGATGATAGT GCGTATGCTC CGGCCATGCC GCGGGTTTGC 
GCATTTGATA TTGAAACCAC AAAGGCTCCA CTGAAGTTTC CACAGCCTGA GGTGGATCAA 
ATTTATATGA TTTCCTACAT GCTTGACGGA CGTGGGTACT TGATTGTCAA TCGTGATATT 
GTTTCCATGG ACATTCATCC GTTTGAGTAC ACCCCAAGGC CGGAGTACGA GGGTATTTTT 
GAAACATTTA ATGAGCCGAA CGAAAAGGCC CTCCTACGGC GCTTTTATGA TGAAATGCGG 
AAGTACAAGC CAAACGTGTA CGTCACGTAC AATGGGGACT ACTTTGATTT TCCTTTTATT 
CATGCTCGAT CCTTATTTCA TCAGTTAAGC ATGCGGGAGG AGATTGGCTT CACGCAGGGC 
GCTGAAGGTG CCTTTTTAAA CCACAAGATT CCCCACCTTG ACTGCTTCTA CTGGGTGAAG 
CGGGACAGCT ACCTCCCACA GGGGAGCCAG GGCCTGAAGG CGGTGACAAA ACACAAATTG 
GGTTACGACC CTATCGAAGT GAATCCGGAG GAAATGTTGC CGCTGGCACA GACGAACCCG 
CAACAGATGG CATCGTACTC CGTCTCCGAT GCGGTGTCAA CATGGTATCT TTATCAGAAG 
TATGTTCACC CTTTTATCTA CTCACTTTCT ACCATCATTC CCATGGCTCC TGATGACGTG 
CTACGAAAAG GATCTGGCGG TCTTTGCGAG TCGCTGCTAA TGGTACAGGC CTACGCAAAC 
AATGTTATAT TTCCGAATAA AAAAGAACAA CAGTTGGAGA AGTTTTTTAA TGGGCATCTG 
ATTGACAGTG AAACATACAT TGGTGGACGC GTGGAAGCTC TTCGAAGTGG GGTGTTTCGT 
AGTGATATTC CCCTCGGTTT TAATATGAAT CCAGACATGT ACCAGAAGCT TATTGATGAT 
CTTGATGATG CGCTACGATT TGCTTTAGAG GTGGAAAATG ACGTCAAGGT AAATGACGTG 
ACGAACTATG AGGAGGTGCA GATGGCAATG AAAGCAAAGT TGGAGTTTTT GAGGGACCAC 
CCACGCCAAT CGAGTGAACC CATCATTTAT CATCTGGACG TAGGCGCCAT GTATCCCAAC 
ATCATCCTTA CCAATAGACT CCAACCGTAT GCCATTCCCA AACCAGATGT CTGTGCAGGA 
TGTTGTTTTA ATTCGCCAAA CAACGAGCAT CAGTGCAAAC GAACCATGAC GTGGAAATGG 
AAAGGGGAAT TTCTGACCGC TAGTCGTCAT GAATATCAAA GAGTAAAGGC TCAGCTGGAG 
AATGAATCTT TTGCAGCAGC CGCGATCCAG CAGGCAAATC TTTCTGCCGT TCAAAAGAAA 
ACTTATGGCA ACCGCAAAGG AAATGTCTTG GAGGGGACGA CATTTGAGCG AAAGCAGCGC 
CCCTTTTCTC GAAGAAAAAC CGATGACTTT AGTGGAGGAT ATCGACAAGA GAGCAAACAC 
CAGCGAAAAG AAGCACGTAA TGCCCTACTT TCAAAAGAAT TTGAAGGAAA AGGCGGGAGT 
GGAAGTGATG ACGAGGACGA GGAAGATGAA GAGGCAGGCG GGTTTAAGGC TTTTCATAAA 
CTTCAGGACA CCACCCAGTT TAATCTTTTG AAGAGGCGTC TTGCGGAGTA TAGTCGGAAG 
GCTTACGGTA AAATTCATGA ATCGCGTGAA ATTTTACGCA CGGATGTTGT ATGCCAGCGC 
GAGAACTCAT TCTACGTGGA TACGGTCCGT TTGTTTCGCG ATCGGCGTTA CGAGTATAAA 
GCCGCCCTTA AGAAGTGGAA GAAACGACTA GACGCGGCCA AAGAAGTAGA AGAAGTCAAA 
CTTTGCAAAA GTCGCTGTGT CCAGATGGAA TCCCTTCAGT TAGCTCACAA ATGTATCCTC 
AACTCTTTCT ACGGCTATGT CATGCGGAAG GGCAGCCGTT GGTATAGCAT GGAAATGGCT 
GGTATCGTAA CATACCTTGG AGCTAGACTC ATTCAGATGG CACGGGCACT TGTGCAACAA 
ATTGGTGTGG CGCTTGAACT TGACACGGAT GGCATATGGT GTTGTCTTCC CAAGGAATTT 
CCAGAAAATT TTATCTTTAC GACCTCACAT CCTTTAAAGT CAAAGGTGAC AATTTCATAT 
CCATGCGTTG TTCTTAACAA AATGGTGCAC GATGATTACA CGAATCACCA ATACCAAACA 
TGCATTGCCC CTGGCGTGTA TGAAAAACGC AGCGAGTGTT CCATTTATTT TGAGGTGGAC 
GGGCCTTACT TGGCTATGTT GTTACCAGCA AGTCGGGAAG AAGGAAAAAG CATTAAAAAG 
CGCTACGCTG TGTTTAATCC GGATGGAGGA CTTGCCGAAT TGAAGGGATT TGAACTAAAG 
CGACGTGGCG AGCTGATGCT TATTAAAGAC TTTCAGTCGC AGGTATTTTG TCGGTTTCTT 
GACGGGAACA GTCTTGTGGA TTCCTACGCC TCCGCCGCCG TGGTCGCCAA CGCGGCACTA 
GACATGTTGT ACTCAAAAGG GGAGGGTTAT GAGACGGAAG AAATTCTTGA GAAACTATCG 
GAAAGCAGCA ACATGACACG ACGTCTCGCC GAGTACCCGG ACTCACAGAA GTCACTGGCG 
CTAACCACGG CACGCCGCAT AGCGGAGTTT TTGGGTCCAC AAATGGTGAA GGATAAGGGA 
TTGGCGTGTC AATTTGTTAT TTCGCGTCTT CCTGCCGGTC GCCCAGTAAC GGAGCGTCCG 
ATTCCTCTCA CCATTTTTCG TGCTGACCCG ACATTGCGTT TGCACTTCCT TCGCAAGTGG 
ACAGGGGACA ACTCACTGTC GCTGGACGTT GACCTCCGTC AGCTGTTAGA CTGGGATTAC 
TACATTGCAA GATTCAACGC ATGTGTGCAG AAGATAATTA CGATTCCCGC GGCACTGCAA 
AAAGTACCAA ACCCAGTACC ACGTGTGCCC TATCCAGAAT GGTTGCATAA GCGACTTCAA 
CAGCAAAATT CTAGGTTTCG GCAAGTTAGT CTAACCGGGA TGCTTGCAAA AATGAAGGCA 
AATGAGAGCG CCATCCCGGA CGTGGAGGAG CTCATTCCAC TCAATAAAGC GGGGGTACCC 
TGTACGGTGA CGCTGGCAAG GAGGAATACG AAAAAGAATG GTGTGAAGGA AGAAGATTTC 
GAAAGCGATT GTGGAGAGGA TCTCGAGAGT GTATTCGATG ATGATGTCGA TCGTATACGG 
CAGGAGGAAG AAGCACGGGA GGAGGAGGCG GTTGATGCCC TCAAAGCAAA GTACTTCTCC 
CCGAAGAGTA CTGTTGCGCT GGACACAGCG TTCTTTACGG CTTCTGGTTT GAAGACGTGG 
CTGCAGCGTC AGAGGGACGC ATGGACGCAT CGTGCGAAAC TTCGTCGAGA GATGCTGCTT 
GATGCAAACG ATACCCTTCT TGTTGATGGT ACTGCCGTCA GCAATCATTT TATTGATATT 
AAGTCCAAGG CACTGACAGC GACGTGGCAC ATCTTGGAGG TGCGATCCGA TAAGGAAGAA 
ACAGGAATGG TGACTGTTAT TGCCGCACTT GAAAAAACGT TACATACATT TCGCTGCGTC 
GTCCCACGTC GTGTTATTGT GGATGCCACT CTACAGTTTC AGGCTCCTGG TGCGAAGGGG 
TTAAATAATG CTCGGATACT ACCTCGAGGT CGTCTTGCAA CAAATTTACA GCAGATTGAC 
ATTCCACCTG GATCTAAGGG GGAGCGTGTG CTCAATGCGC TGCTGAGTAC GCGGGAGGAT 
GTCCTTGCCG TTTACGAAAA TCACATTACC CGAAGCGACA CTTTTATTGA GAAAATTGGC 
TGCTGTGCAA ACGTCATAGC GGACGTTCAT TTGCGCGGTT CACGACGTCG GCCATCCATG 
CGAGACGTGT TTGAACTCGA GGAGTTGGAA AGTACGGCGA GTAGCAATTA TCTTCAGAAT 
TCCACCGATC GATTTGTTTT TATTTTTCAT GTGGCTTTTG ATACCCGTGG TTTGCTTGGC 
GTGGTCAATC ACCACGCAAA AACGGCTGTG GTGGTTACTG TTCAACCTGC ATCGGCACCG 
ATTCCAACAA TAAATTGGAG TTCTCTTCTT TTGGAGGTGA CAAAAGCCAT GAATGCGGCA 
GTGAACCCAC TTGAATTTAT GACGGAAGTT GCATCTGATA TTACAAGCGC ATGGAAACTT 
ATTTACAGAG TCCTGATGGA AGTGATGGAA AGTGGGAGAC CGCCCTTGTT TGCTGTTCTT 
GAGAGTGGCG TGCCGACTCT GCAGCTTTTA ACGCAACGCT GTCTTCCTCA GTCACTGCCG 
TATTTGCGCG TGCTTGGTGC TGTGGAGGAT GAACGACTGT TAGCAGATCC ATTTCGTTGG 
ACGCGTTTAC TGGCGAAACG GCTTTTACAA CGGTATTTTG CTTCTCTTCT TTGGATAGAA 
GAACGTATCG CATTTAGTCG ACTTTCGGGT ATTCCTCTCT GTAATCTTGC GCAGGACACA 
TGTGCGCATG CGTGGGATGT CATGTTTAGT CGTTCACTTC ACTCTCGCTC TCATGTCCTG 
TGGAATTCGC ACGATATATC CATTGCCTAC GAGGCTGTGG AAGAGCGTTC TCGTCAGGTC 
GTTATGCCGG GTGGGTACTT TTCATGGTGT GTAGAGTTTA GTTTAGCCAG GTTGGATGTG 
GTTGCCGTGT TATTCTCGCA GATAATTCAA GAAGAGGATG GCCTCAATGT GCACACTCTC 
TGCGATCGCG GTGTGACTAC ACACTTCAAT ATCTTGCGGG ATCTTGTTTC AGATCTACTC 
GGTCAAGTGG TGGCAAACCC GGTGGCGGAC GCCCTTCTTG CAAGCTTTGC ACGCTGGATT 
CGTGACCCTG TTTCCTGTTG CTATGAACCT CGGTTGCTAG AACTTGTAAG TAAGCTTGCT 
CATCGAGCCC TCACCAATGT TCTTATCCGT CTGGCAAAAT TGGGTGGACG TACCGTAAAG 
GTGGATGGGG ATTCGATTGT TGTGCTTACC CCTAAGCATA CCATTCAGGA TGCAGTTAAT 
TTTGCTCGTT ACATGGTGGA TTCGTTACAG GACCAACCCA TGTTACTTCT CTTGTCTCTT 
CAGCCTATTC GATATTGGTG TCCATTTATG GTTCTTGACA AGCGAGATTT TGCATGCTTA 
TGCCTTTCTA GTGAAGATGC TCTTGGTTTG AAGGAGAGGG AGCAAATGCC ACCTGAAAGG 
CTGAGGGTTG AAAATGCGTT TACAATCTGG GGTAGGCTGC CCAATAAGGT GAAGAGTGTC 
TTTTTTCGGC GCGTTGAAAG CACACTACGG CTCATTGCAG AGATACGTGA AAAGGTGTTT 
CAGGAAATTC AAGCAGACCC CACGGTTGTT CTTGCCACCA AAAGTGAGCA AATCCTATTA 
AGACAAGTGA AGGCCTTTAA GGCTGCAATT GAGAATAAAC TGCATGGTGA ACTTATCGAT 
GAGGTGCATG GACTCATGGA ACGCAAAGAC TTGTATGAGG AAGGTGAAGT TCCGAGCGGT 
GATACACCCT TGTCGGGTAT TGCAGTGGCG TTGGAGTACA CTAAGACCAT TTGTCGTTTA 
CTCGATTTAT TCCACAATGA TGGGGTTGTG GGGAAGATGC ACAATAACTG TTTGCGCCTT 
TGTGGGGTTA GTCCCTTTTC CCCGTTAGCC CAGCTGATTC CAGATGTCGA AGCAGAATGT 
CGCTTGCTAA TGGTGAGGTG CAGTTTTTGC AACCACGATC TCTTTCTCGA CTTGGGCGTG 
CAGGAAAAAC GTTTGCGCTG CGATTGGTGC CATGCGCCAA TTTCCACATC CGCCTTGGAG 
TCCCACCTTG TGCGACGTGT CAACGACCTC GTTATGAACC ATTTGCAGCA GGACTTTAAG 
TGCAGCAAGT GCAATGAGGT TGCGTCGAGC TTCATAAGTC AGAGTTGTTG CGGTCCTCTT 
GTAGGAAAAG GCAAGCCGAT CGCCTCACAG CTCCAGGCAT TGCGCAAGTT TGCCCTCATT 
CAGGACTTTC CATGGTTAAA AGAGAGCGTT GATTTGGCGC TTCGCTTCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_817603.1
CDS	1..6714
Translation	MPVAPNEWKGMESGSRTGWLFNYHPTTVMVADESSVERQGGSGGRAALVLLLQDHDGEIFFVNLFYRPYIFVSVVEGHEHEVELGLMSLFGPQLIYNVETVEKEDLDLVNHLSGRRRLYLKVVFRNVQDLTVVRGRLEKFVKRNASMGATNPLLNPFEGSLRDVMAKTFEDDPFDSNISSERWVDWVQEMREYDVKYHMRVAIDLGIFVGVWYDVIVHEGEAKVTRCDDSAYAPAMPRVCAFDIETTKAPLKFPQPEVDQIYMISYMLDGRGYLIVNRDIVSMDIHPFEYTPRPEYEGIFETFNEPNEKALLRRFYDEMRKYKPNVYVTYNGDYFDFPFIHARSLFHQLSMREEIGFTQGAEGAFLNHKIPHLDCFYWVKRDSYLPQGSQGLKAVTKHKLGYDPIEVNPEEMLPLAQTNPQQMASYSVSDAVSTWYLYQKYVHPFIYSLSTIIPMAPDDVLRKGSGGLCESLLMVQAYANNVIFPNKKEQQLEKFFNGHLIDSETYIGGRVEALRSGVFRSDIPLGFNMNPDMYQKLIDDLDDALRFALEVENDVKVNDVTNYEEVQMAMKAKLEFLRDHPRQSSEPIIYHLDVGAMYPNIILTNRLQPYAIPKPDVCAGCCFNSPNNEHQCKRTMTWKWKGEFLTASRHEYQRVKAQLENESFAAAAIQQANLSAVQKKTYGNRKGNVLEGTTFERKQRPFSRRKTDDFSGGYRQESKHQRKEARNALLSKEFEGKGGSGSDDEDEEDEEAGGFKAFHKLQDTTQFNLLKRRLAEYSRKAYGKIHESREILRTDVVCQRENSFYVDTVRLFRDRRYEYKAALKKWKKRLDAAKEVEEVKLCKSRCVQMESLQLAHKCILNSFYGYVMRKGSRWYSMEMAGIVTYLGARLIQMARALVQQIGVALELDTDGIWCCLPKEFPENFIFTTSHPLKSKVTISYPCVVLNKMVHDDYTNHQYQTCIAPGVYEKRSECSIYFEVDGPYLAMLLPASREEGKSIKKRYAVFNPDGGLAELKGFELKRRGELMLIKDFQSQVFCRFLDGNSLVDSYASAAVVANAALDMLYSKGEGYETEEILEKLSESSNMTRRLAEYPDSQKSLALTTARRIAEFLGPQMVKDKGLACQFVISRLPAGRPVTERPIPLTIFRADPTLRLHFLRKWTGDNSLSLDVDLRQLLDWDYYIARFNACVQKIITIPAALQKVPNPVPRVPYPEWLHKRLQQQNSRFRQVSLTGMLAKMKANESAIPDVEELIPLNKAGVPCTVTLARRNTKKNGVKEEDFESDCGEDLESVFDDDVDRIRQEEEAREEEAVDALKAKYFSPKSTVALDTAFFTASGLKTWLQRQRDAWTHRAKLRREMLLDANDTLLVDGTAVSNHFIDIKSKALTATWHILEVRSDKEETGMVTVIAALEKTLHTFRCVVPRRVIVDATLQFQAPGAKGLNNARILPRGRLATNLQQIDIPPGSKGERVLNALLSTREDVLAVYENHITRSDTFIEKIGCCANVIADVHLRGSRRRPSMRDVFELEELESTASSNYLQNSTDRFVFIFHVAFDTRGLLGVVNHHAKTAVVVTVQPASAPIPTINWSSLLLEVTKAMNAAVNPLEFMTEVASDITSAWKLIYRVLMEVMESGRPPLFAVLESGVPTLQLLTQRCLPQSLPYLRVLGAVEDERLLADPFRWTRLLAKRLLQRYFASLLWIEERIAFSRLSGIPLCNLAQDTCAHAWDVMFSRSLHSRSHVLWNSHDISIAYEAVEERSRQVVMPGGYFSWCVEFSLARLDVVAVLFSQIIQEEDGLNVHTLCDRGVTTHFNILRDLVSDLLGQVVANPVADALLASFARWIRDPVSCCYEPRLLELVSKLAHRALTNVLIRLAKLGGRTVKVDGDSIVVLTPKHTIQDAVNFARYMVDSLQDQPMLLLLSLQPIRYWCPFMVLDKRDFACLCLSSEDALGLKEREQMPPERLRVENAFTIWGRLPNKVKSVFFRRVESTLRLIAEIREKVFQEIQADPTVVLATKSEQILLRQVKAFKAAIENKLHGELIDEVHGLMERKDLYEEGEVPSGDTPLSGIAVALEYTKTICRLLDLFHNDGVVGKMHNNCLRLCGVSPFSPLAQLIPDVEAECRLLMVRCSFCNHDLFLDLGVQEKRLRCDWCHAPISTSALESHLVRRVNDLVMNHLQQDFKCSKCNEVASSFISQSCCGPLVGKGKPIASQLQALRKFALIQDFPWLKESVDLALRFS

Target ORF information:

RefSeq Version	XM_812510.1
Organism	Trypanosoma cruzi strain CL Brener
Definition	Trypanosoma cruzi strain CL Brener DNA polymerase epsilon catalytic subunit partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_812510.1

ORF Insert Sequence:

ATGCCAGTTG CGCCCAATGA ATGGAAGGGA ATGGAGAGCG GCAGCAGGAC CGGCTGGCTA 
TTCAATTACC ACCCAACGAC GGTGATGGTG GCTGACGAGT CGTCAGTGGA GCGCCAAGGT 
GGATCGGGTG GGCGGGCCGC ACTTGTTCTT CTTCTGCAGG ATCATGACGG CGAAATATTT 
TTTGTGAATC TGTTCTATCG CCCCTACATT TTCGTCTCAG TGGTGGAAGG GCATGAACAC 
GAGGTGGAGC TTGGTCTCAT GTCTCTTTTT GGCCCGCAGC TGATTTACAA CGTTGAAACG 
GTCGAAAAGG AGGATTTAGA CCTTGTCAAC CACTTAAGCG GTCGGCGGCG GCTCTATCTG 
AAGGTTGTTT TTAGAAACGT GCAGGACTTA ACGGTGGTGC GTGGTCGACT TGAGAAATTT 
GTGAAGCGCA ACGCCTCCAT GGGGGCTACA AACCCGTTGC TAAACCCTTT TGAAGGATCA 
CTGAGAGATG TGATGGCCAA AACATTTGAG GATGACCCGT TCGATTCAAA CATTTCATCG 
GAGCGATGGG TTGACTGGGT GCAGGAGATG AGGGAATACG ACGTGAAGTA TCACATGCGG 
GTGGCCATTG ATTTGGGCAT TTTTGTTGGT GTGTGGTACG ATGTCATTGT GCATGAAGGC 
GAGGCGAAAG TGACACGCTG TGATGATAGT GCGTATGCTC CGGCCATGCC GCGGGTTTGC 
GCATTTGATA TTGAAACCAC AAAGGCTCCA CTGAAGTTTC CACAGCCTGA GGTGGATCAA 
ATTTATATGA TTTCCTACAT GCTTGACGGA CGTGGGTACT TGATTGTCAA TCGTGATATT 
GTTTCCATGG ACATTCATCC GTTTGAGTAC ACCCCAAGGC CGGAGTACGA GGGTATTTTT 
GAAACATTTA ATGAGCCGAA CGAAAAGGCC CTCCTACGGC GCTTTTATGA TGAAATGCGG 
AAGTACAAGC CAAACGTGTA CGTCACGTAC AATGGGGACT ACTTTGATTT TCCTTTTATT 
CATGCTCGAT CCTTATTTCA TCAGTTAAGC ATGCGGGAGG AGATTGGCTT CACGCAGGGC 
GCTGAAGGTG CCTTTTTAAA CCACAAGATT CCCCACCTTG ACTGCTTCTA CTGGGTGAAG 
CGGGACAGCT ACCTCCCACA GGGGAGCCAG GGCCTGAAGG CGGTGACAAA ACACAAATTG 
GGTTACGACC CTATCGAAGT GAATCCGGAG GAAATGTTGC CGCTGGCACA GACGAACCCG 
CAACAGATGG CATCGTACTC CGTCTCCGAT GCGGTGTCAA CATGGTATCT TTATCAGAAG 
TATGTTCACC CTTTTATCTA CTCACTTTCT ACCATCATTC CCATGGCTCC TGATGACGTG 
CTACGAAAAG GATCTGGCGG TCTTTGCGAG TCGCTGCTAA TGGTACAGGC CTACGCAAAC 
AATGTTATAT TTCCGAATAA AAAAGAACAA CAGTTGGAGA AGTTTTTTAA TGGGCATCTG 
ATTGACAGTG AAACATACAT TGGTGGACGC GTGGAAGCTC TTCGAAGTGG GGTGTTTCGT 
AGTGATATTC CCCTCGGTTT TAATATGAAT CCAGACATGT ACCAGAAGCT TATTGATGAT 
CTTGATGATG CGCTACGATT TGCTTTAGAG GTGGAAAATG ACGTCAAGGT AAATGACGTG 
ACGAACTATG AGGAGGTGCA GATGGCAATG AAAGCAAAGT TGGAGTTTTT GAGGGACCAC 
CCACGCCAAT CGAGTGAACC CATCATTTAT CATCTGGACG TAGGCGCCAT GTATCCCAAC 
ATCATCCTTA CCAATAGACT CCAACCGTAT GCCATTCCCA AACCAGATGT CTGTGCAGGA 
TGTTGTTTTA ATTCGCCAAA CAACGAGCAT CAGTGCAAAC GAACCATGAC GTGGAAATGG 
AAAGGGGAAT TTCTGACCGC TAGTCGTCAT GAATATCAAA GAGTAAAGGC TCAGCTGGAG 
AATGAATCTT TTGCAGCAGC CGCGATCCAG CAGGCAAATC TTTCTGCCGT TCAAAAGAAA 
ACTTATGGCA ACCGCAAAGG AAATGTCTTG GAGGGGACGA CATTTGAGCG AAAGCAGCGC 
CCCTTTTCTC GAAGAAAAAC CGATGACTTT AGTGGAGGAT ATCGACAAGA GAGCAAACAC 
CAGCGAAAAG AAGCACGTAA TGCCCTACTT TCAAAAGAAT TTGAAGGAAA AGGCGGGAGT 
GGAAGTGATG ACGAGGACGA GGAAGATGAA GAGGCAGGCG GGTTTAAGGC TTTTCATAAA 
CTTCAGGACA CCACCCAGTT TAATCTTTTG AAGAGGCGTC TTGCGGAGTA TAGTCGGAAG 
GCTTACGGTA AAATTCATGA ATCGCGTGAA ATTTTACGCA CGGATGTTGT ATGCCAGCGC 
GAGAACTCAT TCTACGTGGA TACGGTCCGT TTGTTTCGCG ATCGGCGTTA CGAGTATAAA 
GCCGCCCTTA AGAAGTGGAA GAAACGACTA GACGCGGCCA AAGAAGTAGA AGAAGTCAAA 
CTTTGCAAAA GTCGCTGTGT CCAGATGGAA TCCCTTCAGT TAGCTCACAA ATGTATCCTC 
AACTCTTTCT ACGGCTATGT CATGCGGAAG GGCAGCCGTT GGTATAGCAT GGAAATGGCT 
GGTATCGTAA CATACCTTGG AGCTAGACTC ATTCAGATGG CACGGGCACT TGTGCAACAA 
ATTGGTGTGG CGCTTGAACT TGACACGGAT GGCATATGGT GTTGTCTTCC CAAGGAATTT 
CCAGAAAATT TTATCTTTAC GACCTCACAT CCTTTAAAGT CAAAGGTGAC AATTTCATAT 
CCATGCGTTG TTCTTAACAA AATGGTGCAC GATGATTACA CGAATCACCA ATACCAAACA 
TGCATTGCCC CTGGCGTGTA TGAAAAACGC AGCGAGTGTT CCATTTATTT TGAGGTGGAC 
GGGCCTTACT TGGCTATGTT GTTACCAGCA AGTCGGGAAG AAGGAAAAAG CATTAAAAAG 
CGCTACGCTG TGTTTAATCC GGATGGAGGA CTTGCCGAAT TGAAGGGATT TGAACTAAAG 
CGACGTGGCG AGCTGATGCT TATTAAAGAC TTTCAGTCGC AGGTATTTTG TCGGTTTCTT 
GACGGGAACA GTCTTGTGGA TTCCTACGCC TCCGCCGCCG TGGTCGCCAA CGCGGCACTA 
GACATGTTGT ACTCAAAAGG GGAGGGTTAT GAGACGGAAG AAATTCTTGA GAAACTATCG 
GAAAGCAGCA ACATGACACG ACGTCTCGCC GAGTACCCGG ACTCACAGAA GTCACTGGCG 
CTAACCACGG CACGCCGCAT AGCGGAGTTT TTGGGTCCAC AAATGGTGAA GGATAAGGGA 
TTGGCGTGTC AATTTGTTAT TTCGCGTCTT CCTGCCGGTC GCCCAGTAAC GGAGCGTCCG 
ATTCCTCTCA CCATTTTTCG TGCTGACCCG ACATTGCGTT TGCACTTCCT TCGCAAGTGG 
ACAGGGGACA ACTCACTGTC GCTGGACGTT GACCTCCGTC AGCTGTTAGA CTGGGATTAC 
TACATTGCAA GATTCAACGC ATGTGTGCAG AAGATAATTA CGATTCCCGC GGCACTGCAA 
AAAGTACCAA ACCCAGTACC ACGTGTGCCC TATCCAGAAT GGTTGCATAA GCGACTTCAA 
CAGCAAAATT CTAGGTTTCG GCAAGTTAGT CTAACCGGGA TGCTTGCAAA AATGAAGGCA 
AATGAGAGCG CCATCCCGGA CGTGGAGGAG CTCATTCCAC TCAATAAAGC GGGGGTACCC 
TGTACGGTGA CGCTGGCAAG GAGGAATACG AAAAAGAATG GTGTGAAGGA AGAAGATTTC 
GAAAGCGATT GTGGAGAGGA TCTCGAGAGT GTATTCGATG ATGATGTCGA TCGTATACGG 
CAGGAGGAAG AAGCACGGGA GGAGGAGGCG GTTGATGCCC TCAAAGCAAA GTACTTCTCC 
CCGAAGAGTA CTGTTGCGCT GGACACAGCG TTCTTTACGG CTTCTGGTTT GAAGACGTGG 
CTGCAGCGTC AGAGGGACGC ATGGACGCAT CGTGCGAAAC TTCGTCGAGA GATGCTGCTT 
GATGCAAACG ATACCCTTCT TGTTGATGGT ACTGCCGTCA GCAATCATTT TATTGATATT 
AAGTCCAAGG CACTGACAGC GACGTGGCAC ATCTTGGAGG TGCGATCCGA TAAGGAAGAA 
ACAGGAATGG TGACTGTTAT TGCCGCACTT GAAAAAACGT TACATACATT TCGCTGCGTC 
GTCCCACGTC GTGTTATTGT GGATGCCACT CTACAGTTTC AGGCTCCTGG TGCGAAGGGG 
TTAAATAATG CTCGGATACT ACCTCGAGGT CGTCTTGCAA CAAATTTACA GCAGATTGAC 
ATTCCACCTG GATCTAAGGG GGAGCGTGTG CTCAATGCGC TGCTGAGTAC GCGGGAGGAT 
GTCCTTGCCG TTTACGAAAA TCACATTACC CGAAGCGACA CTTTTATTGA GAAAATTGGC 
TGCTGTGCAA ACGTCATAGC GGACGTTCAT TTGCGCGGTT CACGACGTCG GCCATCCATG 
CGAGACGTGT TTGAACTCGA GGAGTTGGAA AGTACGGCGA GTAGCAATTA TCTTCAGAAT 
TCCACCGATC GATTTGTTTT TATTTTTCAT GTGGCTTTTG ATACCCGTGG TTTGCTTGGC 
GTGGTCAATC ACCACGCAAA AACGGCTGTG GTGGTTACTG TTCAACCTGC ATCGGCACCG 
ATTCCAACAA TAAATTGGAG TTCTCTTCTT TTGGAGGTGA CAAAAGCCAT GAATGCGGCA 
GTGAACCCAC TTGAATTTAT GACGGAAGTT GCATCTGATA TTACAAGCGC ATGGAAACTT 
ATTTACAGAG TCCTGATGGA AGTGATGGAA AGTGGGAGAC CGCCCTTGTT TGCTGTTCTT 
GAGAGTGGCG TGCCGACTCT GCAGCTTTTA ACGCAACGCT GTCTTCCTCA GTCACTGCCG 
TATTTGCGCG TGCTTGGTGC TGTGGAGGAT GAACGACTGT TAGCAGATCC ATTTCGTTGG 
ACGCGTTTAC TGGCGAAACG GCTTTTACAA CGGTATTTTG CTTCTCTTCT TTGGATAGAA 
GAACGTATCG CATTTAGTCG ACTTTCGGGT ATTCCTCTCT GTAATCTTGC GCAGGACACA 
TGTGCGCATG CGTGGGATGT CATGTTTAGT CGTTCACTTC ACTCTCGCTC TCATGTCCTG 
TGGAATTCGC ACGATATATC CATTGCCTAC GAGGCTGTGG AAGAGCGTTC TCGTCAGGTC 
GTTATGCCGG GTGGGTACTT TTCATGGTGT GTAGAGTTTA GTTTAGCCAG GTTGGATGTG 
GTTGCCGTGT TATTCTCGCA GATAATTCAA GAAGAGGATG GCCTCAATGT GCACACTCTC 
TGCGATCGCG GTGTGACTAC ACACTTCAAT ATCTTGCGGG ATCTTGTTTC AGATCTACTC 
GGTCAAGTGG TGGCAAACCC GGTGGCGGAC GCCCTTCTTG CAAGCTTTGC ACGCTGGATT 
CGTGACCCTG TTTCCTGTTG CTATGAACCT CGGTTGCTAG AACTTGTAAG TAAGCTTGCT 
CATCGAGCCC TCACCAATGT TCTTATCCGT CTGGCAAAAT TGGGTGGACG TACCGTAAAG 
GTGGATGGGG ATTCGATTGT TGTGCTTACC CCTAAGCATA CCATTCAGGA TGCAGTTAAT 
TTTGCTCGTT ACATGGTGGA TTCGTTACAG GACCAACCCA TGTTACTTCT CTTGTCTCTT 
CAGCCTATTC GATATTGGTG TCCATTTATG GTTCTTGACA AGCGAGATTT TGCATGCTTA 
TGCCTTTCTA GTGAAGATGC TCTTGGTTTG AAGGAGAGGG AGCAAATGCC ACCTGAAAGG 
CTGAGGGTTG AAAATGCGTT TACAATCTGG GGTAGGCTGC CCAATAAGGT GAAGAGTGTC 
TTTTTTCGGC GCGTTGAAAG CACACTACGG CTCATTGCAG AGATACGTGA AAAGGTGTTT 
CAGGAAATTC AAGCAGACCC CACGGTTGTT CTTGCCACCA AAAGTGAGCA AATCCTATTA 
AGACAAGTGA AGGCCTTTAA GGCTGCAATT GAGAATAAAC TGCATGGTGA ACTTATCGAT 
GAGGTGCATG GACTCATGGA ACGCAAAGAC TTGTATGAGG AAGGTGAAGT TCCGAGCGGT 
GATACACCCT TGTCGGGTAT TGCAGTGGCG TTGGAGTACA CTAAGACCAT TTGTCGTTTA 
CTCGATTTAT TCCACAATGA TGGGGTTGTG GGGAAGATGC ACAATAACTG TTTGCGCCTT 
TGTGGGGTTA GTCCCTTTTC CCCGTTAGCC CAGCTGATTC CAGATGTCGA AGCAGAATGT 
CGCTTGCTAA TGGTGAGGTG CAGTTTTTGC AACCACGATC TCTTTCTCGA CTTGGGCGTG 
CAGGAAAAAC GTTTGCGCTG CGATTGGTGC CATGCGCCAA TTTCCACATC CGCCTTGGAG 
TCCCACCTTG TGCGACGTGT CAACGACCTC GTTATGAACC ATTTGCAGCA GGACTTTAAG 
TGCAGCAAGT GCAATGAGGT TGCGTCGAGC TTCATAAGTC AGAGTTGTTG CGGTCCTCTT 
GTAGGAAAAG GCAAGCCGAT CGCCTCACAG CTCCAGGCAT TGCGCAAGTT TGCCCTCATT 
CAGGACTTTC CATGGTTAAA AGAGAGCGTT GATTTGGCGC TTCGCTTCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
Science (New York, N.Y.)309(5733)409-15(2005 Jul)
El-Sayed NM,Myler PJ,Bartholomeu DC,Nilsson D,Aggarwal G,Tran AN,Ghedin E,Worthey EA,Delcher AL,Blandin G,Westenberger SJ,Caler E,Cerqueira GC,Branche C,Haas B,Anupama A,Arner E,Aslund L,Attipoe P,Bontempi E,Bringaud F,Burton P,Cadag E,Campbell DA,Carrington M,Crabtree J,Darban H,da Silveira JF,de Jong P,Edwards K,Englund PT,Fazelina G,Feldblyum T,Ferella M,Frasch AC,Gull K,Horn D,Hou L,Huang Y,Kindlund E,Klingbeil M,Kluge S,Koo H,Lacerda D,Levin MJ,Lorenzi H,Louie T,Machado CR,McCulloch R,McKenna A,Mizuno Y,Mottram JC,Nelson S,Ochaya S,Osoegawa K,Pai G,Parsons M,Pentony M,Pettersson U,Pop M,Ramirez JL,Rinta J,Robertson L,Salzberg SL,Sanchez DO,Seyler A,Sharma R,Shetty J,Simpson AJ,Sisk E,Tammi MT,Tarleton R,Teixeira S,Van Aken S,Vogt C,Ward PN,Wickstead B,Wortman J,White O,Fraser CM,Stuart KD,Andersson B

Comparative genomics of trypanosomatid parasitic protozoa.
Science (New York, N.Y.)309(5733)404-9(2005 Jul)
El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N

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Tc00.1047053506147.180 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

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