Tc00.1047053509537.50 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

Gene
Clones
gRNAs

Summary

Gene Symbol	Tc00.1047053509537.50
Entrez Gene ID	3542861
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Trypanosoma cruzi strain CL Brener

mRNA and Protein(s)

mRNA	Protein	Name
XM_806738.1	XP_811831.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Worthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K, Englund PT, Fazelina G, Feldblyum T, Ferella M, Frasch AC, Gull K, Horn D, Hou L, Huang Y, Kindlund E, Klingbeil M, Kluge S, Koo H, Lacerda D, Levin MJ, Lorenzi H, Louie T, Machado CR, McCulloch R, McKenna A, Mizuno Y, Mottram JC, Nelson S, Ochaya S, Osoegawa K, Pai G, Parsons M, Pentony M, Pettersson U, Pop M, Ramirez JL, Rinta J, Robertson L, Salzberg SL, Sanchez DO, Seyler A, Sharma R, Shetty J, Simpson AJ, Sisk E, Tammi MT, Tarleton R, Teixeira S, Van Aken S, Vogt C, Ward PN, Wickstead B, Wortman J, White O, Fraser CM, Stuart KD, Andersson B
Science (New York, N.Y.)309(5733)409-15(2005 Jul)

Comparative genomics of trypanosomatid parasitic protozoa.
El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran AN, Wortman JR, Alsmark UC, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N
Science (New York, N.Y.)309(5733)404-9(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tc00.1047053509537.50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tc00.1047053509537.50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTb06814	XM_806738.1 Latest version!	Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tc00.1047053509537.50 ( XM_806738.1 ) cDNA ORF clone, Trypanosoma cruzi strain CL Brener -> XP_811831.1 Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.

Price & Availability↑

CloneID	OTb06814
Clone ID Related Accession (Same CDS sequence)	XM_806738.1
Accession Version	XM_806738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1225382400000
Organism	Trypanosoma cruzi strain CL Brener
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001849178). COMPLETENESS: incomplete on both ends.

ATGCAGGAGC TCAGGGAAGC AAATAAGTTA GAAAGCGTAT TACTCTCCCT GAGAAGTCTA 
CAAATCACGG AGGGGATGAT GAATACAACG CTGGTGTTGA TTAAACCGCA CGCGTGCCGA 
GAAAAATTCC TTGACGTTGC GCGTGAACAC TTTGACCAAT ATGGAGTTCG CACGGATGAC 
ACGATGCTTT TGTCTGGTTC TCAGGTTGAG CGCGGGGCCT ACGTGGAGCG CCACTACAGC 
TCGGTGCATG CTCTGGCTGT ATGCTCCTTG GAGGACCTGA GTGCATTTGT GTCGGAAGAA 
ACTGCGTCTC TTTTTTTCTC GGCATTCGGT GAGCTGTGGG ATGCTGCAGT GGAGGAGCGG 
CGGGTGATGA CACCTGACGA TGCCATGACA ATTCTTGGCC TCTCCCCAGA AGAGTTAAAT 
GCGCGGTGGT GTGCCTCGAA GTCATGTGCC CGGCTTGAGT ACGGTTTTTA TGTCTCTTAT 
TTGGAGGAGG AGCGGGTGTA TGTAGTCAAC GGCTTCTACC CAAGTCTGCT GGGTTCCTTC 
ACCGCAACCG ATAGTCAAAC GTGCCTTTTT GTTCTTTCTT GGCCGGAGTC GATGTACACG 
TGGAAGCAAT TCAACCTGGA GGTTCTTGGA GCGGCGAACC CCAGCGAGGC GGCGCCGACG 
TCTCTGCGTC GTTTGTTGTT TGAAAACTGG AGGGAATATG GACTGAGTGA GCAGCCGAGC 
CTGATGCATA ACGGCCTGGA CGCCTCCAGC GGGCCACTCG AGGCGCTGGC ACATCGCTCT 
GTGTGGATGC ATCGGCGAGC CACGGAGGAC GATTTTGGAC GAGCGCTTCT TCAAGAGGGG 
GTTTCACTTG AGTTTTTGGA ACAGTTGTTG AAGAACCCCA CCATAACGTA CGGTGGGGAG 
ACCCGACCGA TTTTTGAGCT TCTGGAGGAC TTGCAGAGCA GCGAGGTGAT ACACCACTTG 
GCGGTTTTGT ACGCCGCGGA GAAGTTAAAA AGAACGAACC AGGCGTCCGT TGGATTCGGC 
ACGAGCAACA CCATCTCGGG TGCCGCGGAA TGGACCATTG TGCTTGACGA TGAGGATGCT 
GAAGAGAGAC GCAACAGAGC CTTAGTTTTT GTAAAACCGC ATGCCAACAC ACCTGAAACA 
AGGGCACTTG TGGAGGAGCG TCTCATGCAA ACCCGTGGAA TGCAAATCGT CAGTCAACGT 
CACGTTTTTG GCGGTGAAAT TGCGGCACAG CAGTTGATGT ACAAACACTA CCGAACCATA 
GCCCGTTACG CCGTCAAGGT TTCGCCGATG TCTATTAATG TAAGCACGCA GAACAGAGCC 
CTTTTTAAGG AAGTGTTTGG TATTGCTTGG AAAGAAGCAG TTTGTTCTGG TCGTGTCTGG 
AATGCTGAAA CAGCCATACA CACTCTTGGT GAAATCTCCG CCGTTGAGCT TTACGGGATG 
TGGGGTTCAT GTACGAAGAC AATGAAAATT GCCTCAGGAG CGTACGTGGC TCAATTTTTA 
AATGAAAAGG TTTTTGTGAT CAACGGGTTT TACCCTTATC TGCGCGATAC GTACGGCGCT 
CAAGATGCAA AGGTGACATG CTACCTTGTT TCCTGGCCAG AGGCGTGCAT GACATGGCGT 
GCGTTCCGTG AGGAGTTGAT TGGAAGCACA AATCCCGGCA ACGCACCTCC CAACAGCCTG 
CGCGGTCTCA TACGAGACCG GTGGCAAGAA TTGGGCCTGC AATACCCGCC GACGACAACC 
GATAACGGGG TTCACGCATC TGCAGGGCCG TTTGAGGCGC TGCTGGAACG CCATCTATGG 
ATGCACTTAC CACTCTCCCA TGATCCTCTC ACGCTGCGAC TGCAGGAATG CGGCCTAACA 
GGGGCTCTGT TGTACCGATG GGCTTCCAAC CCAGAGGTAA TGCTGCGCGG GAAGAAGCTC 
TCCGGGTGTG TGTTTGACCT CCTGGAAAAC ATGCAAACCT CCGAGATGGT GGACATCATG 
CGCGAGGCGG AGCAACAGAC AATGGCATTG TACAAGGAAA CGCCGATGAA TCGTGCGGTG 
TTGATTCTTA AGCCCTTTGC TGTGAACGAG CGCACAATTG CGGCGGTAAA AAAAACTCTC 
GAGTCGGTAG GTCTGCTGGT GACGCGGGAG ATGTCCGTTT TTAGTGCTCG CATTGTGAAG 
TGCTATCTGA ACTCTGCTGC GTTTTGCGCC GCGACTCGCC TTGCCGAAAT CAATTCTTCG 
ACTCCTCAAG AAGTTGTCAG CCCTGCCATC AAGGACCGCT TTTGTGAGAT CTTTCACTCA 
ACATGGGATT ATTGTGTGGT TGACGGAAGT CTGATGGGTG CCACGACTGC ATGCGAGAAT 
TTGGGTCTCA CTCCAAAAGA GCTCTTGCAG CTGTGGGAGG CGTCGAGCCC CAAGAAAGTT 
GGACGGGCGT GCTACATAGC ATTCTTGAAG GCTCAAGGCG TTTTTGTCAT CAATGGGTTT 
GTTCCCTTTA CCCGGGAGTG CTACGGAAGG CCTGGTAGCC GAGTTTATTT ATTTGAGTTG 
GAGTGGAAGG AAAGCGCATG GACATGGAGA GACTTTTGCG AGGTTTTGAT TGGAGATTCT 
TCCAGTCCAC AGAACGCTGC TCAGGGCAGC CTGCATCGTA CCTTTGCAGA CGAATGGTCG 
AAATTTGGTC TGCAGCAGCA GCCAGTGTCC AGGGCGACGA GTGCGCTCTA CGCGTCGGAG 
GGACCGTTGG AGGCTGCTGC CGACCGCGAG ATGTGGCTGG ATGTGGATTT GCCGGAGGAC 
TCGTTTGTAC AGCATCTGTT GCTCGACGGT GTGCCACTCT CATTGTTGCT CCCGTACGTG 
CGTAACGAAC ATCATGGCGG CTCCGTTTCG CAAGGCCCCC AGCAGCAAGA GCATGAGGGA 
AATGGTTTAC TAGCTGGTGC TATACCTGAA TACACACGGA GCATGTTGTC GAAGCATCAG 
CAGAGCAGTG AGGTAGTTCG CCGGATGAAG GCGCTCACTT CGCGTTTCCT GCGAGCCATA 
AAAATGAATT ATGCATTTAT TTGGGTTAAA CCACATGCAG GCACCCCGGA GGTGTTGAAA 
TGGATCCCAT CCGCCCTGCA AGAGCACCGT CTCGAAGTTG TCGCCTCCGG ACACCTGATG 
ATGGAAGAGG TAATTGAAAA ACATCTTGTT GATCAGCAGT ATAATGCCCT TTATCGCAAC 
GCCATGGGGC GGAGTGCGGC GGAGGTGCCT ATCTCTGCTA AGCAAAAGGT GGAATTTGAG 
CGCACCTTTG GCATCACTTG GAGCACCGCT CTAAAACTCC ACATGATCAT CAACGCTGCA 
GCAGCTGAGG AAATAATGGG CGTTGTCGAA TTGTTGGAGG CTTGGAACAG GGCCACTCGC 
AAGGTCTGTC TTTCCGCTGA TCTTTATGTG GCCTACCTCG AAGTGGAAGG CCTCTATGTT 
ATCAATGGGT TCTACCCCTA CCTCCGTTCC CGCATGTATA CCGGATCCCG CATCTATTGG 
TATGTGGTGA TGTGGGATGG GGAGATCATG ACATGGGATG AATTCCAGAG GTATGTTATT 
GGTGGAGACG TTCCGGCCAC GGCGGAGTCG GCCTCTCTGC GAAACTTGCT GTGGGCATCG 
TGGGTAGAGT TGGGTCTGCC TCAGCAGCCT GACGGTGTTG ACAACGGGTT TCATGCCTCC 
GCTTCTCCAA TAGAGGGACT TGCAGATCGC GTCGCATGGC TTGGGACGAG TGTGGAGGAC 
GATGTCTTCG GACGGCTTCT CATTCAGGGA GGGGTGAGCG CCGAGTACCT GCGCACTCTT 
TTGCGGAATC CATTGGTGCG CCACCGCGAC GAGCCTGTAG ACGCTGTGGG TGATGTTTTT 
GAGAAGCTCT GCACGGAGAA TCCCCTTTTG ATTGCACAAC TTGTTGGACT TCAGTCGACT 
GGCGGCATGC ATCTCATATC TAATCGCGCC TGCTCCGGTG CGGCATTCTC TCGACCGCCC 
CTTTCCAGAC AAGGGACACC CATTGAGAAG ACCAGCACGA ATAAGAACAC CAACGAACCG 
GCGGTAGAGA TGGTGTGGCA AACTAATGAA GCTGCAGGTG TCGTCTCTCC TCCTTCTCCT 
CCTGCCGCTG CCACCGCCAC CAAGACACCC AGGCGCAACT ATGCGTTTCT CCTTGTAAAT 
CCCAAATGTT TGTCTACTGA GGGAGAAGAG CTTACAACGG CTTACATCAC GGACTTTCTG 
CAGCGCCACA AGATACGGGT TGAGGCAGGG GGAAGGGTAA GGGCCATTGC GCCAGCACTG 
CGACATGTGT GGGAAGAACT TCAAGCCGAT ACTTTTAGAT ACGCCGTGAA GCAGACACCT 
TCTCAATATG CTATTGGCGG GGATGCAATT TCTGCTTTTG AAGCTGCCTT TGGCGAGAGC 
TGGGATCCTG AAAAAATTTG GAATGCTCTG GATACCGCTT TGGAGTTTAA TTTGTCTGCG 
CTGCGCCTGA AAGAATTGTG GGATGGCTGT GCACTGCGAA AACGAATAGC ACCTTTTCTC 
TACATTGGGA AGATGCGAGA TCGTGGTTTG TACCTTGTCA ATGGATTTGC TCTCCACAGC 
ACAGAGGTGT TTAACTTGTC ATCTACCATA AAACGGTATT TCTTGCTTTC ATGGGACGAT 
GCCGACGCTG ACTACGCTCA ATATCTACGT CATTTCATTG GCGATTCATA TGTGGAGCAG 
GCAGAGCCTG GCAGTCTGCA ACGGCTGCTT TGCGATGATT GGAAAAACGC TGGACTAAAA 
TGCCCGCCTG ATCCTTTTGA AGGGGCTGTA ATGACATCTA CAAGTTCCCT GGAGGCCATA 
CAGCAGCGAC AGCTGTGGCT CTCGTTGGGT TTGTTGCGAG ATCCAGTGGG CTGCTTTGCG 
GTGAGGCAGT TGGACGTTTC ACCCTATGTG TTGCGCTGGG CTGTGAGCAA CCCCCGCTCA 
AGGCGAGGCG ACGGGCTCTA CTTGTTTGAG ACAGTGCGTG GACAAGGCTC GGCGGATGCA 
CTGCAGCTAC TTAAGGCTGA AGACACACGG CATCGTGCTG CGCCACTACG CAACTCTGCT 
TTTGTGCTTG TTAAGAGTCA CGCCCTGTGC CGTTCTTTTG CCATGACAAT TGAGATGGTG 
CTTTCAAATG CAAATGTGCG TATCGATGAA GAGGGGGATT TAAGCGGTTT AGTTGTACGT 
GGGAGGGGAC TTGTGCAGCA CTTGTATGCC ACCGCATCCA GGTATGCGGT GCGTGAGGTA 
TCAACCATCC GTTTGACCGA GGTGGAACGT GAGAGGGTGC GTCGGGAGCT TGGCGCTGAT 
TGGAAGGAGC TTCTGCGTGG GGGCGTCATT GTGAACGCGT ACCAGGCCCT GGAGGTGCTT 
GGTGGTGTTA CGCCGCAGTA TCTTTACGGC TGTTGGAAAA GATCCAGTCG GAAGTTAGTC 
ATTCGCCCGA ATTTTGTCGT TGCGGAGCTT TCGGAGTATG GCATCTTCGT TGTGAATGGC 
TTCTTCCCGG AGTTGAAGAA TTCCCTTGAG TCCACCACCG CTCTGCTTCA TTGGTATGTC 
GTCTCATGGT GTGAAGAGGA GATGAGTTGG CCCGCCTTTC TTGATCAGGT CATTGGGGAT 
GATTGCCCGA GCGTCGCGGT GCCGCAAAGC ATTCGTGGAC AGCTTTTTCA ACGATGGAGA 
GAGTACGGCC TTTCCATGGA GCCGGATTTA CTGCACAACG GCATCCATGT AAGCGAGGGT 
GCGCTTCAGG CAATGCGAGA TCGCACCAAA TCCCTTAACT GTCCGTTGAG CGAGGACTAT 
CTTGGCGATG CGATGTTACG CCACGGTGTG CCGGAGAGGA TTCTGCGTGT GTGGCTTGAC 
AATCCCGACG TCACTTCAGG CGGAGTTGAG GCAGGCGTGT TTGAACATCT GCGGCACTGC 
GACACCTCGC GCCTGTTGAT GTTGCTGACT GCGCTTACAG AGGAGTTGCG CTGCGAGGAA 
AGCGACGGGA CGACAGTACA GGCAGCCCGT ATTCCACTCG TAGAGAACAA CGACGGCCTC 
ACTGCACCAC TTCAAGACGG CTCACAGGAG GGCCACACAC GGGGAATCGA AAACGACAAC 
AACAAGAACG AGTCGGTCTT GGGAAAATTT TTGGATGAAG CGGAAGGGGC GCTTCTTTTT 
CGAAACACCG CCGTGATTAT TTTGAAACCA CACGCCTCCG AAAATCTTGG AGTCATGGCG 
CTGCTGGAGC GCATCCTTAC TGGAAACAAC ATTCGCGTAA GACGGGAGCT CCGCAAGCGA 
ATTAATCCCC GAATAGTAGA TCAGCATTTC TCTTCACCCA TGTACTACGC CATGCAGAAG 
TACCACACGG CATTCCCAGA GGTAACAGAG GAGGGTCGAG TGCGTTTCTA CGAGGCCTTT 
GGAGAAGAGT GGGAGCACGC AATCGCCTCT CAGCGTGTGA TGGGTGCCTT CAACGCCCTC 
AAGCTTTTTT CAATGACCCC ATCCCAACTC TATGTGAAGT GGTCACTGTC AGAACAAGGG 
CACGTGCAAC TTGCCTTTGA TATGGAGGTG GTGAAGTTCT ATGCGGAAGG TGTTTATGTC 
GTCAATGCAA CTATTCCTTT GGAGCGCGAA AGGATGATTC AGGTGGGGCA GGTTACGCAG 
GATGTCCACT GCTATTTGGT GAGTTGGGAC CAGCGAGAGT GCAGTTGGCA GAAGTTTCTT 
TATGAGGTTA TTGGCAATAC GGATCCGGCT ACTGCCGCAG AGATTTCGTT GCGTGGTCAA 
CTTTACAATG ACTGGGAGAA ATTTGGCCTA CCATCCAGGC CCAACCGCAT CGACAATATT 
GTTCTGGCAA GCTCTGGACC ACTGCAGGCA TTCATGGAGC GCGAGCTTTG GTGCAGTACG 
GCCGATTTGG TTCCTGATCC ACTTGTTCGA GCCCTCTGTT ATGTCGACTA TGACTCCAGC 
CAACTATTCG AATGGAAGGA AAATCCTCTA GTGCAATACA CAGGCAAAAA GCTGGGCAAA 
CATTCTGTGG CGATGCGCAT GTTTGGTTTT ACTCATGAAA TGGACACAAG AGAGTTTCTC 
GAACTCATGG CTGAGCATGA TGGCTTTTTT GTTCCCACTG TCCCCCCGTG TGAGATGGAG 
GATGCGCAAG ACCAGTCGTA CGAGTCATTC GAGATGAAGG CTGACAGAAC TGTAAAGAGG 
TCTGGAAAGA GGCAGGCCCA TCGTTCGGCC AAAACATTGC CCTTCCGCAT GAGGGACGAG 
AGATCGGTGG ACTGGCTGCA GGAGGCGCTG TTGAAGGCGG ACGAGGACGA TGTGGTGCGT 
CTGTGGGAAT ATTATGGTGG CATTGCAGAT CGAGAGGAGG AGGAGGAGGA GGAGGAGGGA 
GCAGCTGGTG CGTATGCCGA TGAGTTGTTC TCCACCACGG GTCTGCATCG AAGGCTGCGG 
GGGACGATTA ACGGTGAGGC CTTCCGGCGG GATATTGAGG CGCTGGATTG CTTTGGGCTT 
CCGCTCTCCA GGTACAAGGT GAAAAAACTG TTTGAGGGCA TGGCGTGGCG TGGGGATGGA 
CGCATAACAC ACGAGGAGTT CCGCGTGGCG ATGGCGGTAC TGCAGCAGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_811831.1
CDS	1..7554
Translation	MQELREANKLESVLLSLRSLQITEGMMNTTLVLIKPHACREKFLDVAREHFDQYGVRTDDTMLLSGSQVERGAYVERHYSSVHALAVCSLEDLSAFVSEETASLFFSAFGELWDAAVEERRVMTPDDAMTILGLSPEELNARWCASKSCARLEYGFYVSYLEEERVYVVNGFYPSLLGSFTATDSQTCLFVLSWPESMYTWKQFNLEVLGAANPSEAAPTSLRRLLFENWREYGLSEQPSLMHNGLDASSGPLEALAHRSVWMHRRATEDDFGRALLQEGVSLEFLEQLLKNPTITYGGETRPIFELLEDLQSSEVIHHLAVLYAAEKLKRTNQASVGFGTSNTISGAAEWTIVLDDEDAEERRNRALVFVKPHANTPETRALVEERLMQTRGMQIVSQRHVFGGEIAAQQLMYKHYRTIARYAVKVSPMSINVSTQNRALFKEVFGIAWKEAVCSGRVWNAETAIHTLGEISAVELYGMWGSCTKTMKIASGAYVAQFLNEKVFVINGFYPYLRDTYGAQDAKVTCYLVSWPEACMTWRAFREELIGSTNPGNAPPNSLRGLIRDRWQELGLQYPPTTTDNGVHASAGPFEALLERHLWMHLPLSHDPLTLRLQECGLTGALLYRWASNPEVMLRGKKLSGCVFDLLENMQTSEMVDIMREAEQQTMALYKETPMNRAVLILKPFAVNERTIAAVKKTLESVGLLVTREMSVFSARIVKCYLNSAAFCAATRLAEINSSTPQEVVSPAIKDRFCEIFHSTWDYCVVDGSLMGATTACENLGLTPKELLQLWEASSPKKVGRACYIAFLKAQGVFVINGFVPFTRECYGRPGSRVYLFELEWKESAWTWRDFCEVLIGDSSSPQNAAQGSLHRTFADEWSKFGLQQQPVSRATSALYASEGPLEAAADREMWLDVDLPEDSFVQHLLLDGVPLSLLLPYVRNEHHGGSVSQGPQQQEHEGNGLLAGAIPEYTRSMLSKHQQSSEVVRRMKALTSRFLRAIKMNYAFIWVKPHAGTPEVLKWIPSALQEHRLEVVASGHLMMEEVIEKHLVDQQYNALYRNAMGRSAAEVPISAKQKVEFERTFGITWSTALKLHMIINAAAAEEIMGVVELLEAWNRATRKVCLSADLYVAYLEVEGLYVINGFYPYLRSRMYTGSRIYWYVVMWDGEIMTWDEFQRYVIGGDVPATAESASLRNLLWASWVELGLPQQPDGVDNGFHASASPIEGLADRVAWLGTSVEDDVFGRLLIQGGVSAEYLRTLLRNPLVRHRDEPVDAVGDVFEKLCTENPLLIAQLVGLQSTGGMHLISNRACSGAAFSRPPLSRQGTPIEKTSTNKNTNEPAVEMVWQTNEAAGVVSPPSPPAAATATKTPRRNYAFLLVNPKCLSTEGEELTTAYITDFLQRHKIRVEAGGRVRAIAPALRHVWEELQADTFRYAVKQTPSQYAIGGDAISAFEAAFGESWDPEKIWNALDTALEFNLSALRLKELWDGCALRKRIAPFLYIGKMRDRGLYLVNGFALHSTEVFNLSSTIKRYFLLSWDDADADYAQYLRHFIGDSYVEQAEPGSLQRLLCDDWKNAGLKCPPDPFEGAVMTSTSSLEAIQQRQLWLSLGLLRDPVGCFAVRQLDVSPYVLRWAVSNPRSRRGDGLYLFETVRGQGSADALQLLKAEDTRHRAAPLRNSAFVLVKSHALCRSFAMTIEMVLSNANVRIDEEGDLSGLVVRGRGLVQHLYATASRYAVREVSTIRLTEVERERVRRELGADWKELLRGGVIVNAYQALEVLGGVTPQYLYGCWKRSSRKLVIRPNFVVAELSEYGIFVVNGFFPELKNSLESTTALLHWYVVSWCEEEMSWPAFLDQVIGDDCPSVAVPQSIRGQLFQRWREYGLSMEPDLLHNGIHVSEGALQAMRDRTKSLNCPLSEDYLGDAMLRHGVPERILRVWLDNPDVTSGGVEAGVFEHLRHCDTSRLLMLLTALTEELRCEESDGTTVQAARIPLVENNDGLTAPLQDGSQEGHTRGIENDNNKNESVLGKFLDEAEGALLFRNTAVIILKPHASENLGVMALLERILTGNNIRVRRELRKRINPRIVDQHFSSPMYYAMQKYHTAFPEVTEEGRVRFYEAFGEEWEHAIASQRVMGAFNALKLFSMTPSQLYVKWSLSEQGHVQLAFDMEVVKFYAEGVYVVNATIPLERERMIQVGQVTQDVHCYLVSWDQRECSWQKFLYEVIGNTDPATAAEISLRGQLYNDWEKFGLPSRPNRIDNIVLASSGPLQAFMERELWCSTADLVPDPLVRALCYVDYDSSQLFEWKENPLVQYTGKKLGKHSVAMRMFGFTHEMDTREFLELMAEHDGFFVPTVPPCEMEDAQDQSYESFEMKADRTVKRSGKRQAHRSAKTLPFRMRDERSVDWLQEALLKADEDDVVRLWEYYGGIADREEEEEEEEGAAGAYADELFSTTGLHRRLRGTINGEAFRRDIEALDCFGLPLSRYKVKKLFEGMAWRGDGRITHEEFRVAMAVLQQM

Target ORF information:

RefSeq Version	XM_806738.1
Organism	Trypanosoma cruzi strain CL Brener
Definition	Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_806738.1

ORF Insert Sequence:

ATGCAGGAGC TCAGGGAAGC AAATAAGTTA GAAAGCGTAT TACTCTCCCT GAGAAGTCTA 
CAAATCACGG AGGGGATGAT GAATACAACG CTGGTGTTGA TTAAACCGCA CGCGTGCCGA 
GAAAAATTCC TTGACGTTGC GCGTGAACAC TTTGACCAAT ATGGAGTTCG CACGGATGAC 
ACGATGCTTT TGTCTGGTTC TCAGGTTGAG CGCGGGGCCT ACGTGGAGCG CCACTACAGC 
TCGGTGCATG CTCTGGCTGT ATGCTCCTTG GAGGACCTGA GTGCATTTGT GTCGGAAGAA 
ACTGCGTCTC TTTTTTTCTC GGCATTCGGT GAGCTGTGGG ATGCTGCAGT GGAGGAGCGG 
CGGGTGATGA CACCTGACGA TGCCATGACA ATTCTTGGCC TCTCCCCAGA AGAGTTAAAT 
GCGCGGTGGT GTGCCTCGAA GTCATGTGCC CGGCTTGAGT ACGGTTTTTA TGTCTCTTAT 
TTGGAGGAGG AGCGGGTGTA TGTAGTCAAC GGCTTCTACC CAAGTCTGCT GGGTTCCTTC 
ACCGCAACCG ATAGTCAAAC GTGCCTTTTT GTTCTTTCTT GGCCGGAGTC GATGTACACG 
TGGAAGCAAT TCAACCTGGA GGTTCTTGGA GCGGCGAACC CCAGCGAGGC GGCGCCGACG 
TCTCTGCGTC GTTTGTTGTT TGAAAACTGG AGGGAATATG GACTGAGTGA GCAGCCGAGC 
CTGATGCATA ACGGCCTGGA CGCCTCCAGC GGGCCACTCG AGGCGCTGGC ACATCGCTCT 
GTGTGGATGC ATCGGCGAGC CACGGAGGAC GATTTTGGAC GAGCGCTTCT TCAAGAGGGG 
GTTTCACTTG AGTTTTTGGA ACAGTTGTTG AAGAACCCCA CCATAACGTA CGGTGGGGAG 
ACCCGACCGA TTTTTGAGCT TCTGGAGGAC TTGCAGAGCA GCGAGGTGAT ACACCACTTG 
GCGGTTTTGT ACGCCGCGGA GAAGTTAAAA AGAACGAACC AGGCGTCCGT TGGATTCGGC 
ACGAGCAACA CCATCTCGGG TGCCGCGGAA TGGACCATTG TGCTTGACGA TGAGGATGCT 
GAAGAGAGAC GCAACAGAGC CTTAGTTTTT GTAAAACCGC ATGCCAACAC ACCTGAAACA 
AGGGCACTTG TGGAGGAGCG TCTCATGCAA ACCCGTGGAA TGCAAATCGT CAGTCAACGT 
CACGTTTTTG GCGGTGAAAT TGCGGCACAG CAGTTGATGT ACAAACACTA CCGAACCATA 
GCCCGTTACG CCGTCAAGGT TTCGCCGATG TCTATTAATG TAAGCACGCA GAACAGAGCC 
CTTTTTAAGG AAGTGTTTGG TATTGCTTGG AAAGAAGCAG TTTGTTCTGG TCGTGTCTGG 
AATGCTGAAA CAGCCATACA CACTCTTGGT GAAATCTCCG CCGTTGAGCT TTACGGGATG 
TGGGGTTCAT GTACGAAGAC AATGAAAATT GCCTCAGGAG CGTACGTGGC TCAATTTTTA 
AATGAAAAGG TTTTTGTGAT CAACGGGTTT TACCCTTATC TGCGCGATAC GTACGGCGCT 
CAAGATGCAA AGGTGACATG CTACCTTGTT TCCTGGCCAG AGGCGTGCAT GACATGGCGT 
GCGTTCCGTG AGGAGTTGAT TGGAAGCACA AATCCCGGCA ACGCACCTCC CAACAGCCTG 
CGCGGTCTCA TACGAGACCG GTGGCAAGAA TTGGGCCTGC AATACCCGCC GACGACAACC 
GATAACGGGG TTCACGCATC TGCAGGGCCG TTTGAGGCGC TGCTGGAACG CCATCTATGG 
ATGCACTTAC CACTCTCCCA TGATCCTCTC ACGCTGCGAC TGCAGGAATG CGGCCTAACA 
GGGGCTCTGT TGTACCGATG GGCTTCCAAC CCAGAGGTAA TGCTGCGCGG GAAGAAGCTC 
TCCGGGTGTG TGTTTGACCT CCTGGAAAAC ATGCAAACCT CCGAGATGGT GGACATCATG 
CGCGAGGCGG AGCAACAGAC AATGGCATTG TACAAGGAAA CGCCGATGAA TCGTGCGGTG 
TTGATTCTTA AGCCCTTTGC TGTGAACGAG CGCACAATTG CGGCGGTAAA AAAAACTCTC 
GAGTCGGTAG GTCTGCTGGT GACGCGGGAG ATGTCCGTTT TTAGTGCTCG CATTGTGAAG 
TGCTATCTGA ACTCTGCTGC GTTTTGCGCC GCGACTCGCC TTGCCGAAAT CAATTCTTCG 
ACTCCTCAAG AAGTTGTCAG CCCTGCCATC AAGGACCGCT TTTGTGAGAT CTTTCACTCA 
ACATGGGATT ATTGTGTGGT TGACGGAAGT CTGATGGGTG CCACGACTGC ATGCGAGAAT 
TTGGGTCTCA CTCCAAAAGA GCTCTTGCAG CTGTGGGAGG CGTCGAGCCC CAAGAAAGTT 
GGACGGGCGT GCTACATAGC ATTCTTGAAG GCTCAAGGCG TTTTTGTCAT CAATGGGTTT 
GTTCCCTTTA CCCGGGAGTG CTACGGAAGG CCTGGTAGCC GAGTTTATTT ATTTGAGTTG 
GAGTGGAAGG AAAGCGCATG GACATGGAGA GACTTTTGCG AGGTTTTGAT TGGAGATTCT 
TCCAGTCCAC AGAACGCTGC TCAGGGCAGC CTGCATCGTA CCTTTGCAGA CGAATGGTCG 
AAATTTGGTC TGCAGCAGCA GCCAGTGTCC AGGGCGACGA GTGCGCTCTA CGCGTCGGAG 
GGACCGTTGG AGGCTGCTGC CGACCGCGAG ATGTGGCTGG ATGTGGATTT GCCGGAGGAC 
TCGTTTGTAC AGCATCTGTT GCTCGACGGT GTGCCACTCT CATTGTTGCT CCCGTACGTG 
CGTAACGAAC ATCATGGCGG CTCCGTTTCG CAAGGCCCCC AGCAGCAAGA GCATGAGGGA 
AATGGTTTAC TAGCTGGTGC TATACCTGAA TACACACGGA GCATGTTGTC GAAGCATCAG 
CAGAGCAGTG AGGTAGTTCG CCGGATGAAG GCGCTCACTT CGCGTTTCCT GCGAGCCATA 
AAAATGAATT ATGCATTTAT TTGGGTTAAA CCACATGCAG GCACCCCGGA GGTGTTGAAA 
TGGATCCCAT CCGCCCTGCA AGAGCACCGT CTCGAAGTTG TCGCCTCCGG ACACCTGATG 
ATGGAAGAGG TAATTGAAAA ACATCTTGTT GATCAGCAGT ATAATGCCCT TTATCGCAAC 
GCCATGGGGC GGAGTGCGGC GGAGGTGCCT ATCTCTGCTA AGCAAAAGGT GGAATTTGAG 
CGCACCTTTG GCATCACTTG GAGCACCGCT CTAAAACTCC ACATGATCAT CAACGCTGCA 
GCAGCTGAGG AAATAATGGG CGTTGTCGAA TTGTTGGAGG CTTGGAACAG GGCCACTCGC 
AAGGTCTGTC TTTCCGCTGA TCTTTATGTG GCCTACCTCG AAGTGGAAGG CCTCTATGTT 
ATCAATGGGT TCTACCCCTA CCTCCGTTCC CGCATGTATA CCGGATCCCG CATCTATTGG 
TATGTGGTGA TGTGGGATGG GGAGATCATG ACATGGGATG AATTCCAGAG GTATGTTATT 
GGTGGAGACG TTCCGGCCAC GGCGGAGTCG GCCTCTCTGC GAAACTTGCT GTGGGCATCG 
TGGGTAGAGT TGGGTCTGCC TCAGCAGCCT GACGGTGTTG ACAACGGGTT TCATGCCTCC 
GCTTCTCCAA TAGAGGGACT TGCAGATCGC GTCGCATGGC TTGGGACGAG TGTGGAGGAC 
GATGTCTTCG GACGGCTTCT CATTCAGGGA GGGGTGAGCG CCGAGTACCT GCGCACTCTT 
TTGCGGAATC CATTGGTGCG CCACCGCGAC GAGCCTGTAG ACGCTGTGGG TGATGTTTTT 
GAGAAGCTCT GCACGGAGAA TCCCCTTTTG ATTGCACAAC TTGTTGGACT TCAGTCGACT 
GGCGGCATGC ATCTCATATC TAATCGCGCC TGCTCCGGTG CGGCATTCTC TCGACCGCCC 
CTTTCCAGAC AAGGGACACC CATTGAGAAG ACCAGCACGA ATAAGAACAC CAACGAACCG 
GCGGTAGAGA TGGTGTGGCA AACTAATGAA GCTGCAGGTG TCGTCTCTCC TCCTTCTCCT 
CCTGCCGCTG CCACCGCCAC CAAGACACCC AGGCGCAACT ATGCGTTTCT CCTTGTAAAT 
CCCAAATGTT TGTCTACTGA GGGAGAAGAG CTTACAACGG CTTACATCAC GGACTTTCTG 
CAGCGCCACA AGATACGGGT TGAGGCAGGG GGAAGGGTAA GGGCCATTGC GCCAGCACTG 
CGACATGTGT GGGAAGAACT TCAAGCCGAT ACTTTTAGAT ACGCCGTGAA GCAGACACCT 
TCTCAATATG CTATTGGCGG GGATGCAATT TCTGCTTTTG AAGCTGCCTT TGGCGAGAGC 
TGGGATCCTG AAAAAATTTG GAATGCTCTG GATACCGCTT TGGAGTTTAA TTTGTCTGCG 
CTGCGCCTGA AAGAATTGTG GGATGGCTGT GCACTGCGAA AACGAATAGC ACCTTTTCTC 
TACATTGGGA AGATGCGAGA TCGTGGTTTG TACCTTGTCA ATGGATTTGC TCTCCACAGC 
ACAGAGGTGT TTAACTTGTC ATCTACCATA AAACGGTATT TCTTGCTTTC ATGGGACGAT 
GCCGACGCTG ACTACGCTCA ATATCTACGT CATTTCATTG GCGATTCATA TGTGGAGCAG 
GCAGAGCCTG GCAGTCTGCA ACGGCTGCTT TGCGATGATT GGAAAAACGC TGGACTAAAA 
TGCCCGCCTG ATCCTTTTGA AGGGGCTGTA ATGACATCTA CAAGTTCCCT GGAGGCCATA 
CAGCAGCGAC AGCTGTGGCT CTCGTTGGGT TTGTTGCGAG ATCCAGTGGG CTGCTTTGCG 
GTGAGGCAGT TGGACGTTTC ACCCTATGTG TTGCGCTGGG CTGTGAGCAA CCCCCGCTCA 
AGGCGAGGCG ACGGGCTCTA CTTGTTTGAG ACAGTGCGTG GACAAGGCTC GGCGGATGCA 
CTGCAGCTAC TTAAGGCTGA AGACACACGG CATCGTGCTG CGCCACTACG CAACTCTGCT 
TTTGTGCTTG TTAAGAGTCA CGCCCTGTGC CGTTCTTTTG CCATGACAAT TGAGATGGTG 
CTTTCAAATG CAAATGTGCG TATCGATGAA GAGGGGGATT TAAGCGGTTT AGTTGTACGT 
GGGAGGGGAC TTGTGCAGCA CTTGTATGCC ACCGCATCCA GGTATGCGGT GCGTGAGGTA 
TCAACCATCC GTTTGACCGA GGTGGAACGT GAGAGGGTGC GTCGGGAGCT TGGCGCTGAT 
TGGAAGGAGC TTCTGCGTGG GGGCGTCATT GTGAACGCGT ACCAGGCCCT GGAGGTGCTT 
GGTGGTGTTA CGCCGCAGTA TCTTTACGGC TGTTGGAAAA GATCCAGTCG GAAGTTAGTC 
ATTCGCCCGA ATTTTGTCGT TGCGGAGCTT TCGGAGTATG GCATCTTCGT TGTGAATGGC 
TTCTTCCCGG AGTTGAAGAA TTCCCTTGAG TCCACCACCG CTCTGCTTCA TTGGTATGTC 
GTCTCATGGT GTGAAGAGGA GATGAGTTGG CCCGCCTTTC TTGATCAGGT CATTGGGGAT 
GATTGCCCGA GCGTCGCGGT GCCGCAAAGC ATTCGTGGAC AGCTTTTTCA ACGATGGAGA 
GAGTACGGCC TTTCCATGGA GCCGGATTTA CTGCACAACG GCATCCATGT AAGCGAGGGT 
GCGCTTCAGG CAATGCGAGA TCGCACCAAA TCCCTTAACT GTCCGTTGAG CGAGGACTAT 
CTTGGCGATG CGATGTTACG CCACGGTGTG CCGGAGAGGA TTCTGCGTGT GTGGCTTGAC 
AATCCCGACG TCACTTCAGG CGGAGTTGAG GCAGGCGTGT TTGAACATCT GCGGCACTGC 
GACACCTCGC GCCTGTTGAT GTTGCTGACT GCGCTTACAG AGGAGTTGCG CTGCGAGGAA 
AGCGACGGGA CGACAGTACA GGCAGCCCGT ATTCCACTCG TAGAGAACAA CGACGGCCTC 
ACTGCACCAC TTCAAGACGG CTCACAGGAG GGCCACACAC GGGGAATCGA AAACGACAAC 
AACAAGAACG AGTCGGTCTT GGGAAAATTT TTGGATGAAG CGGAAGGGGC GCTTCTTTTT 
CGAAACACCG CCGTGATTAT TTTGAAACCA CACGCCTCCG AAAATCTTGG AGTCATGGCG 
CTGCTGGAGC GCATCCTTAC TGGAAACAAC ATTCGCGTAA GACGGGAGCT CCGCAAGCGA 
ATTAATCCCC GAATAGTAGA TCAGCATTTC TCTTCACCCA TGTACTACGC CATGCAGAAG 
TACCACACGG CATTCCCAGA GGTAACAGAG GAGGGTCGAG TGCGTTTCTA CGAGGCCTTT 
GGAGAAGAGT GGGAGCACGC AATCGCCTCT CAGCGTGTGA TGGGTGCCTT CAACGCCCTC 
AAGCTTTTTT CAATGACCCC ATCCCAACTC TATGTGAAGT GGTCACTGTC AGAACAAGGG 
CACGTGCAAC TTGCCTTTGA TATGGAGGTG GTGAAGTTCT ATGCGGAAGG TGTTTATGTC 
GTCAATGCAA CTATTCCTTT GGAGCGCGAA AGGATGATTC AGGTGGGGCA GGTTACGCAG 
GATGTCCACT GCTATTTGGT GAGTTGGGAC CAGCGAGAGT GCAGTTGGCA GAAGTTTCTT 
TATGAGGTTA TTGGCAATAC GGATCCGGCT ACTGCCGCAG AGATTTCGTT GCGTGGTCAA 
CTTTACAATG ACTGGGAGAA ATTTGGCCTA CCATCCAGGC CCAACCGCAT CGACAATATT 
GTTCTGGCAA GCTCTGGACC ACTGCAGGCA TTCATGGAGC GCGAGCTTTG GTGCAGTACG 
GCCGATTTGG TTCCTGATCC ACTTGTTCGA GCCCTCTGTT ATGTCGACTA TGACTCCAGC 
CAACTATTCG AATGGAAGGA AAATCCTCTA GTGCAATACA CAGGCAAAAA GCTGGGCAAA 
CATTCTGTGG CGATGCGCAT GTTTGGTTTT ACTCATGAAA TGGACACAAG AGAGTTTCTC 
GAACTCATGG CTGAGCATGA TGGCTTTTTT GTTCCCACTG TCCCCCCGTG TGAGATGGAG 
GATGCGCAAG ACCAGTCGTA CGAGTCATTC GAGATGAAGG CTGACAGAAC TGTAAAGAGG 
TCTGGAAAGA GGCAGGCCCA TCGTTCGGCC AAAACATTGC CCTTCCGCAT GAGGGACGAG 
AGATCGGTGG ACTGGCTGCA GGAGGCGCTG TTGAAGGCGG ACGAGGACGA TGTGGTGCGT 
CTGTGGGAAT ATTATGGTGG CATTGCAGAT CGAGAGGAGG AGGAGGAGGA GGAGGAGGGA 
GCAGCTGGTG CGTATGCCGA TGAGTTGTTC TCCACCACGG GTCTGCATCG AAGGCTGCGG 
GGGACGATTA ACGGTGAGGC CTTCCGGCGG GATATTGAGG CGCTGGATTG CTTTGGGCTT 
CCGCTCTCCA GGTACAAGGT GAAAAAACTG TTTGAGGGCA TGGCGTGGCG TGGGGATGGA 
CGCATAACAC ACGAGGAGTT CCGCGTGGCG ATGGCGGTAC TGCAGCAGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
Science (New York, N.Y.)309(5733)409-15(2005 Jul)
El-Sayed NM,Myler PJ,Bartholomeu DC,Nilsson D,Aggarwal G,Tran AN,Ghedin E,Worthey EA,Delcher AL,Blandin G,Westenberger SJ,Caler E,Cerqueira GC,Branche C,Haas B,Anupama A,Arner E,Aslund L,Attipoe P,Bontempi E,Bringaud F,Burton P,Cadag E,Campbell DA,Carrington M,Crabtree J,Darban H,da Silveira JF,de Jong P,Edwards K,Englund PT,Fazelina G,Feldblyum T,Ferella M,Frasch AC,Gull K,Horn D,Hou L,Huang Y,Kindlund E,Klingbeil M,Kluge S,Koo H,Lacerda D,Levin MJ,Lorenzi H,Louie T,Machado CR,McCulloch R,McKenna A,Mizuno Y,Mottram JC,Nelson S,Ochaya S,Osoegawa K,Pai G,Parsons M,Pentony M,Pettersson U,Pop M,Ramirez JL,Rinta J,Robertson L,Salzberg SL,Sanchez DO,Seyler A,Sharma R,Shetty J,Simpson AJ,Sisk E,Tammi MT,Tarleton R,Teixeira S,Van Aken S,Vogt C,Ward PN,Wickstead B,Wortman J,White O,Fraser CM,Stuart KD,Andersson B

Comparative genomics of trypanosomatid parasitic protozoa.
Science (New York, N.Y.)309(5733)404-9(2005 Jul)
El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N

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Tc00.1047053509537.50 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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