Tc00.1047053503925.70 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

Gene
Clones
gRNAs

Summary

Gene Symbol	Tc00.1047053503925.70
Entrez Gene ID	3542433
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Trypanosoma cruzi strain CL Brener

mRNA and Protein(s)

mRNA	Protein	Name
XM_806365.1	XP_811458.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Worthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K, Englund PT, Fazelina G, Feldblyum T, Ferella M, Frasch AC, Gull K, Horn D, Hou L, Huang Y, Kindlund E, Klingbeil M, Kluge S, Koo H, Lacerda D, Levin MJ, Lorenzi H, Louie T, Machado CR, McCulloch R, McKenna A, Mizuno Y, Mottram JC, Nelson S, Ochaya S, Osoegawa K, Pai G, Parsons M, Pentony M, Pettersson U, Pop M, Ramirez JL, Rinta J, Robertson L, Salzberg SL, Sanchez DO, Seyler A, Sharma R, Shetty J, Simpson AJ, Sisk E, Tammi MT, Tarleton R, Teixeira S, Van Aken S, Vogt C, Ward PN, Wickstead B, Wortman J, White O, Fraser CM, Stuart KD, Andersson B
Science (New York, N.Y.)309(5733)409-15(2005 Jul)

Comparative genomics of trypanosomatid parasitic protozoa.
El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran AN, Wortman JR, Alsmark UC, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N
Science (New York, N.Y.)309(5733)404-9(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tc00.1047053503925.70 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tc00.1047053503925.70 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTb06509	XM_806365.1 Latest version!	Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tc00.1047053503925.70 ( XM_806365.1 ) cDNA ORF clone, Trypanosoma cruzi strain CL Brener -> XP_811458.1 Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.

Price & Availability↑

CloneID	OTb06509
Clone ID Related Accession (Same CDS sequence)	XM_806365.1
Accession Version	XM_806365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1225382400000
Organism	Trypanosoma cruzi strain CL Brener
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001849431). COMPLETENESS: incomplete on both ends.

ATGCAGCGGC CAAGTCCGGT GAATTCGACC TCTAAAAAAG TTGTACGTCT CACACGAGTG 
AGACGTTCTG TAGATATGGG TGATAGCGGT AACATTAGCG CTAGTACTGG GACCTCCAGT 
TTACACGACC AGCCTGTGGA GATGCCATGT GCGTCAAATG TGAATCCCCC CATGGATGGT 
ACTGCTGCAT CTGCTTCATC TACTCCTGAC GTCACGCAAG TTGTGAATTC ACTGGAGGAG 
GGCAATGCAT TGGTGAAACG TCTACGGCAG CTGGAGAATG AAAATGCTTC TCTCGCCACT 
GCCCTTACGA AAGTGTGTGA CGATAACGCA AACCTGGCAA AGCATGTAGT CAAGCAAGAG 
GCGAAGTGTG TGGCGCTCTC AACGAGTCCA ATTTCGGCGA CTTCGGTGTT GCGTTCTCTC 
TTTGAGCCTG AGAAGGTTGG CTGGTTTGGT GGCTCGAAGA CGGCAGCGCG TGAAAACTTG 
GAAAAGATGA TGGAGGAGCT TCAAAATGAG TTTCTCGAGT ACCAGGCGAG CCATGCCTCC 
TCCAATGACG AGGTACAGGC ACTCATTGGC GAACTGCAGA GTGCCCACAT GAATGCGCAG 
CAGCTTTCGA TCATTCTGCA TGAGCAACAG GAGGTGATTA GCCGCGGGCC GTTGAGCGTG 
GCGGTCCGCA TTTTTCCTCT TGAGACGGCG GCGGAGAGGG TGTCGCTCGT CGACCTGCGG 
CATGAGTTGG AGCGCCGCTC GGAGCGAGAA AACGAGTTTC GCCAGGAGCT TAGCAGAGCA 
CGAGAGGAGT TGCAGGTGAA AATGGTGGAG AACGCGCGGC TGCAGCGGGA CCTTCAGGCC 
CTGCGTCTGG AGGGCAACCA TAGTAACGAG GTGGAGCGGC AGTACATGAC CGACGAGATC 
TCGCGGCTAA AGACGGAGGT GACGCAGCTG CGCAAGGCGC TGGCGACGGA AGAGCAGCTG 
CATCGACTGA AAGTGGGCGA GAATGCCGCT TTATCGCAGC GATTAAGTGA GACAGAGAGC 
CGTTTACACG CGTCCAACGC GGAAATGCGG GCGCAGCCGT CTGAGGCTCC GTGTGATCGC 
TGCGGTGAGT TGCAGCGGGA GTGTGACGTG GCGAATGAGC GCTTGCAGAC GGTTACGCGG 
GAGATGCAGC AGCTGGGGAT GCGGCTGTCG GAGCTGAAGC AGGTGAACGA GCAGGAGGTG 
AGCGAGCTGC AGCGGGAGAA GTCGGAGCTT GAACAGAAAT TGAACCACAG CGTTGCACGC 
GACACGCTGG TGGCGGCGGA GTCGCAAAAC AAACATCTCC TGCAGGAGCT TGAGTTCACC 
TCTGCGCGGG TGAAGGAACT GGAGAAACTC ATCGAGCGGG AGCGCGAAAA GACCACCAGC 
GATGCCGTCA AGAGCAGCGA TGAGGGCAGA GGGACGCTGA AGGACAGCGA GATCCTTGAA 
GCGCTGGAGC GTAACCGGGA GCGCATCGCG CGGGTTGAAT TTCAACGGGA TCAACTTATT 
GAGGAGGCGA AGCAACTTCG AGCGGTTGCA GTCACGAAGG AGGAGGAACT CGTTCGACTT 
ACCCGCGCGT CACAAGAAGA CAAGCAGCGC ATCAATTTTC TTTTGAAGCG CTGCGTGACG 
TTGACAGGGG ATCTAAACAA CAGCATCGCC GCAAAGGATG CGCTTAATGA GGAGTATGAG 
AATGCGCAGT TGAAGCTTAA AAAGTTGCTT CGTGAAGGGG AGGAAATTGT ACGGCTCCAA 
CAACAAGTCA CGGATCTGAA GAAGGAGAAC GATGCTCTGC AGGATCGTCT CTCAAATGTG 
GAGGTGCTAG AGAATAACCT TGTCAAGGCA AAGGAGACTA TTGCCTATAT GGAGTTAGCA 
AAGAGTCAAA CTATAAACAT GAGTCAATAC GCCGAACTGC AATCCAAGAC GTCTAAGTTG 
GAAAACACTC TGGAGCCCAT CAAGAAACAG CTCGAGGAGG CACGGAAAGA GTTGGGACGC 
ATAAAAAATG AAAACGAGAG TCTACATGCA GTTTTGACAC GACAGCAGAA CACAATTAAG 
TCGCACGAGG AGCAAATGGA AGCGCTTCAG GCAAACGACG CCAAAATGCG GGACAAAACG 
AATCAACTCA CCAGTGAGAA TACACGTCTG CGGGACGTAA ATGAGACGCT GGAAGCACAG 
ATGAACAACA TGCAGGATGG CATCAAGGAG ATGGCGGTGA AGTTGGAGCA GGAGCGCACA 
CGGGCTCAGG TGGCGGCTGA GGCAACACAC AAGGAAGAGA TTGCACAATT GCGGCGTAAA 
CTCGAGTCGG CAAATGCAAC CTTGGCGACG ATGCAATCGA CACAAGAGAA CTTTGTACCG 
GAGGCCATTC ACAAGGCGGC CCTGGCGGAG AAGGGACATG TTAGCGAGGA ACTACGGCAA 
AAGACATTTG ATCTTGACCG GCTAAAGTCT GAAATCCTCA TCTACCGTCA AACGATCAAC 
GACTTGGAGA CAGAGAACGA GGAGCACGTG CGGCAGATTG AGTCTCTGCA AGAAAGCCAT 
AAAAGAGATC TCACTGCCGC GGCAGACCGA CTTTCGGCGG AAACAAAGAA AGTTACGGAG 
GCCAATGAGG CGTTGGAGGG ACTTCAAATG CAGATCAATG AATTTCAACA ACGGGAGGAA 
CAATTGCTAT CGCGCCAGAA GGAAATGGAA GAGGCGGCGC AGAAACACCG TCGGGCCTTT 
GAAGATAAAT GTCGGCAGGA AGAGAAACTT CTTCAAACGG TTACCGCCAC GAACCAGGAG 
ATGGAGACAC AAAATGAGGA GCTGACGAAA GTGAAGAGTG CACTGTTAGA ATACCAACGT 
CAAAATGCGC AGCTGGAGTC CGAATTGAAA TCTGGGGCGG CACAAGGACC GCGTCAACAA 
GAGGAAATGG AGTCTGAGCT TGAAAAGCTC CGTGGCGAAA TTCAACGTGT AAACGCGGAG 
AAGGCTACTT TGGAGATGGA ACTCGGGAAG CTGCGAAGGA CCACAAAAAA TGGTAGTTGG 
GATGGCAATG GTGGTGGAGG TGTGGAGAAT AGTGGTAGTA ACAACAATAC CGATAATAAT 
AGTAATAATA GTAATAGTAA TAATACTGCG ATGAGAGGTA AAGGCGTCGG TGATCATGGT 
GGCCATGTTA ACATGGAAAC TGGGATGTCC TCGGATGCTG CCGTCCAGTA CGCGCGGGCG 
GAGCAGCTGC AGATGTCACT TATTCAGGCC CAGCGTCAAT TGGAAGAGCA GAGAAAGGTT 
AGCGCGGAGG CGGCGGAGAC ATTTGATAGG GCGCTTGACA AGGCAGACGC TGAGGCCGAG 
GCGCTACGTG AGCGAATTCA GCAATTAGAG AGTGATCTCC ATAGAATGTC ACAGGAGAGT 
GACGAGACAC GCAGTTTGTC TTCTGCGCAC CCAAAGAAAC AAAAAAAGAA GAAGGCAGTC 
AAGAAGTCCC TCTCAACAAT GGCCAATATC GATGACCTGC AGGAGACGGA TGTGCTGGCG 
GTGGCTGCCG CGGATGAAGG GGTGCGGCAG CAGCAACAGC AGCAACTCGA GCAGCAACGT 
GAGTCATTAG AGAAACTTGA ACAGGAGTTA TCGACTCTCC GTGATGAAAA TGTGCGACTT 
AAGGAGAATA TTGCAACTCT CACGCAGGAA TTATTAGGGG CTCATGATGA TCTTGAGCTG 
GCAAGAAAAG GTGCTGACTC GAAGACCGCC GAGCTGCGGG GGCAGATTCA GTTATTGGAG 
GCTCAGTTGC GGAACCGCTT GCCCAACACA GATGGAGGAA CCGAGTTGCA CCGTCTTGCG 
GAGGAAAATA GGCAACTTCA ATCGGAGGTG TCTTTACTTT CCGATTCTAT GAATGCGACA 
ACGGGGAATC CTGACGCGAC GGTGCAGCGA CTGAAGGCGG AACTCCAAGC ACTTGCAAAG 
GAGCTTGTGC CGACGAAGAA CAAACTTGTT GAGTACATGG CAATGGCAGA TAGACTGGGC 
ATTCATTATC CCTTTTCACC AGACATGGAG GGGGTTGCTG TCCTTAGGCT TAAAGCAATG 
CATTTTGACA CGGTAACGGG AAGAGTGCGG GCGTGCTCGG CCCCGCGACA ACCATACGCC 
TCGTCTTTTG ACAAAAACGC GTTATCATCA TCGGGTGCCA AGAAGAAAAA AAGAGTGAAG 
AAGCGTCAGC CGCAGGGAAG TCATGTCGAT GAACTTTCAG ATTTGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_811458.1
CDS	1..4191
Translation	MQRPSPVNSTSKKVVRLTRVRRSVDMGDSGNISASTGTSSLHDQPVEMPCASNVNPPMDGTAASASSTPDVTQVVNSLEEGNALVKRLRQLENENASLATALTKVCDDNANLAKHVVKQEAKCVALSTSPISATSVLRSLFEPEKVGWFGGSKTAARENLEKMMEELQNEFLEYQASHASSNDEVQALIGELQSAHMNAQQLSIILHEQQEVISRGPLSVAVRIFPLETAAERVSLVDLRHELERRSERENEFRQELSRAREELQVKMVENARLQRDLQALRLEGNHSNEVERQYMTDEISRLKTEVTQLRKALATEEQLHRLKVGENAALSQRLSETESRLHASNAEMRAQPSEAPCDRCGELQRECDVANERLQTVTREMQQLGMRLSELKQVNEQEVSELQREKSELEQKLNHSVARDTLVAAESQNKHLLQELEFTSARVKELEKLIEREREKTTSDAVKSSDEGRGTLKDSEILEALERNRERIARVEFQRDQLIEEAKQLRAVAVTKEEELVRLTRASQEDKQRINFLLKRCVTLTGDLNNSIAAKDALNEEYENAQLKLKKLLREGEEIVRLQQQVTDLKKENDALQDRLSNVEVLENNLVKAKETIAYMELAKSQTINMSQYAELQSKTSKLENTLEPIKKQLEEARKELGRIKNENESLHAVLTRQQNTIKSHEEQMEALQANDAKMRDKTNQLTSENTRLRDVNETLEAQMNNMQDGIKEMAVKLEQERTRAQVAAEATHKEEIAQLRRKLESANATLATMQSTQENFVPEAIHKAALAEKGHVSEELRQKTFDLDRLKSEILIYRQTINDLETENEEHVRQIESLQESHKRDLTAAADRLSAETKKVTEANEALEGLQMQINEFQQREEQLLSRQKEMEEAAQKHRRAFEDKCRQEEKLLQTVTATNQEMETQNEELTKVKSALLEYQRQNAQLESELKSGAAQGPRQQEEMESELEKLRGEIQRVNAEKATLEMELGKLRRTTKNGSWDGNGGGGVENSGSNNNTDNNSNNSNSNNTAMRGKGVGDHGGHVNMETGMSSDAAVQYARAEQLQMSLIQAQRQLEEQRKVSAEAAETFDRALDKADAEAEALRERIQQLESDLHRMSQESDETRSLSSAHPKKQKKKKAVKKSLSTMANIDDLQETDVLAVAAADEGVRQQQQQQLEQQRESLEKLEQELSTLRDENVRLKENIATLTQELLGAHDDLELARKGADSKTAELRGQIQLLEAQLRNRLPNTDGGTELHRLAEENRQLQSEVSLLSDSMNATTGNPDATVQRLKAELQALAKELVPTKNKLVEYMAMADRLGIHYPFSPDMEGVAVLRLKAMHFDTVTGRVRACSAPRQPYASSFDKNALSSSGAKKKKRVKKRQPQGSHVDELSDLR

Target ORF information:

RefSeq Version	XM_806365.1
Organism	Trypanosoma cruzi strain CL Brener
Definition	Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_806365.1

ORF Insert Sequence:

ATGCAGCGGC CAAGTCCGGT GAATTCGACC TCTAAAAAAG TTGTACGTCT CACACGAGTG 
AGACGTTCTG TAGATATGGG TGATAGCGGT AACATTAGCG CTAGTACTGG GACCTCCAGT 
TTACACGACC AGCCTGTGGA GATGCCATGT GCGTCAAATG TGAATCCCCC CATGGATGGT 
ACTGCTGCAT CTGCTTCATC TACTCCTGAC GTCACGCAAG TTGTGAATTC ACTGGAGGAG 
GGCAATGCAT TGGTGAAACG TCTACGGCAG CTGGAGAATG AAAATGCTTC TCTCGCCACT 
GCCCTTACGA AAGTGTGTGA CGATAACGCA AACCTGGCAA AGCATGTAGT CAAGCAAGAG 
GCGAAGTGTG TGGCGCTCTC AACGAGTCCA ATTTCGGCGA CTTCGGTGTT GCGTTCTCTC 
TTTGAGCCTG AGAAGGTTGG CTGGTTTGGT GGCTCGAAGA CGGCAGCGCG TGAAAACTTG 
GAAAAGATGA TGGAGGAGCT TCAAAATGAG TTTCTCGAGT ACCAGGCGAG CCATGCCTCC 
TCCAATGACG AGGTACAGGC ACTCATTGGC GAACTGCAGA GTGCCCACAT GAATGCGCAG 
CAGCTTTCGA TCATTCTGCA TGAGCAACAG GAGGTGATTA GCCGCGGGCC GTTGAGCGTG 
GCGGTCCGCA TTTTTCCTCT TGAGACGGCG GCGGAGAGGG TGTCGCTCGT CGACCTGCGG 
CATGAGTTGG AGCGCCGCTC GGAGCGAGAA AACGAGTTTC GCCAGGAGCT TAGCAGAGCA 
CGAGAGGAGT TGCAGGTGAA AATGGTGGAG AACGCGCGGC TGCAGCGGGA CCTTCAGGCC 
CTGCGTCTGG AGGGCAACCA TAGTAACGAG GTGGAGCGGC AGTACATGAC CGACGAGATC 
TCGCGGCTAA AGACGGAGGT GACGCAGCTG CGCAAGGCGC TGGCGACGGA AGAGCAGCTG 
CATCGACTGA AAGTGGGCGA GAATGCCGCT TTATCGCAGC GATTAAGTGA GACAGAGAGC 
CGTTTACACG CGTCCAACGC GGAAATGCGG GCGCAGCCGT CTGAGGCTCC GTGTGATCGC 
TGCGGTGAGT TGCAGCGGGA GTGTGACGTG GCGAATGAGC GCTTGCAGAC GGTTACGCGG 
GAGATGCAGC AGCTGGGGAT GCGGCTGTCG GAGCTGAAGC AGGTGAACGA GCAGGAGGTG 
AGCGAGCTGC AGCGGGAGAA GTCGGAGCTT GAACAGAAAT TGAACCACAG CGTTGCACGC 
GACACGCTGG TGGCGGCGGA GTCGCAAAAC AAACATCTCC TGCAGGAGCT TGAGTTCACC 
TCTGCGCGGG TGAAGGAACT GGAGAAACTC ATCGAGCGGG AGCGCGAAAA GACCACCAGC 
GATGCCGTCA AGAGCAGCGA TGAGGGCAGA GGGACGCTGA AGGACAGCGA GATCCTTGAA 
GCGCTGGAGC GTAACCGGGA GCGCATCGCG CGGGTTGAAT TTCAACGGGA TCAACTTATT 
GAGGAGGCGA AGCAACTTCG AGCGGTTGCA GTCACGAAGG AGGAGGAACT CGTTCGACTT 
ACCCGCGCGT CACAAGAAGA CAAGCAGCGC ATCAATTTTC TTTTGAAGCG CTGCGTGACG 
TTGACAGGGG ATCTAAACAA CAGCATCGCC GCAAAGGATG CGCTTAATGA GGAGTATGAG 
AATGCGCAGT TGAAGCTTAA AAAGTTGCTT CGTGAAGGGG AGGAAATTGT ACGGCTCCAA 
CAACAAGTCA CGGATCTGAA GAAGGAGAAC GATGCTCTGC AGGATCGTCT CTCAAATGTG 
GAGGTGCTAG AGAATAACCT TGTCAAGGCA AAGGAGACTA TTGCCTATAT GGAGTTAGCA 
AAGAGTCAAA CTATAAACAT GAGTCAATAC GCCGAACTGC AATCCAAGAC GTCTAAGTTG 
GAAAACACTC TGGAGCCCAT CAAGAAACAG CTCGAGGAGG CACGGAAAGA GTTGGGACGC 
ATAAAAAATG AAAACGAGAG TCTACATGCA GTTTTGACAC GACAGCAGAA CACAATTAAG 
TCGCACGAGG AGCAAATGGA AGCGCTTCAG GCAAACGACG CCAAAATGCG GGACAAAACG 
AATCAACTCA CCAGTGAGAA TACACGTCTG CGGGACGTAA ATGAGACGCT GGAAGCACAG 
ATGAACAACA TGCAGGATGG CATCAAGGAG ATGGCGGTGA AGTTGGAGCA GGAGCGCACA 
CGGGCTCAGG TGGCGGCTGA GGCAACACAC AAGGAAGAGA TTGCACAATT GCGGCGTAAA 
CTCGAGTCGG CAAATGCAAC CTTGGCGACG ATGCAATCGA CACAAGAGAA CTTTGTACCG 
GAGGCCATTC ACAAGGCGGC CCTGGCGGAG AAGGGACATG TTAGCGAGGA ACTACGGCAA 
AAGACATTTG ATCTTGACCG GCTAAAGTCT GAAATCCTCA TCTACCGTCA AACGATCAAC 
GACTTGGAGA CAGAGAACGA GGAGCACGTG CGGCAGATTG AGTCTCTGCA AGAAAGCCAT 
AAAAGAGATC TCACTGCCGC GGCAGACCGA CTTTCGGCGG AAACAAAGAA AGTTACGGAG 
GCCAATGAGG CGTTGGAGGG ACTTCAAATG CAGATCAATG AATTTCAACA ACGGGAGGAA 
CAATTGCTAT CGCGCCAGAA GGAAATGGAA GAGGCGGCGC AGAAACACCG TCGGGCCTTT 
GAAGATAAAT GTCGGCAGGA AGAGAAACTT CTTCAAACGG TTACCGCCAC GAACCAGGAG 
ATGGAGACAC AAAATGAGGA GCTGACGAAA GTGAAGAGTG CACTGTTAGA ATACCAACGT 
CAAAATGCGC AGCTGGAGTC CGAATTGAAA TCTGGGGCGG CACAAGGACC GCGTCAACAA 
GAGGAAATGG AGTCTGAGCT TGAAAAGCTC CGTGGCGAAA TTCAACGTGT AAACGCGGAG 
AAGGCTACTT TGGAGATGGA ACTCGGGAAG CTGCGAAGGA CCACAAAAAA TGGTAGTTGG 
GATGGCAATG GTGGTGGAGG TGTGGAGAAT AGTGGTAGTA ACAACAATAC CGATAATAAT 
AGTAATAATA GTAATAGTAA TAATACTGCG ATGAGAGGTA AAGGCGTCGG TGATCATGGT 
GGCCATGTTA ACATGGAAAC TGGGATGTCC TCGGATGCTG CCGTCCAGTA CGCGCGGGCG 
GAGCAGCTGC AGATGTCACT TATTCAGGCC CAGCGTCAAT TGGAAGAGCA GAGAAAGGTT 
AGCGCGGAGG CGGCGGAGAC ATTTGATAGG GCGCTTGACA AGGCAGACGC TGAGGCCGAG 
GCGCTACGTG AGCGAATTCA GCAATTAGAG AGTGATCTCC ATAGAATGTC ACAGGAGAGT 
GACGAGACAC GCAGTTTGTC TTCTGCGCAC CCAAAGAAAC AAAAAAAGAA GAAGGCAGTC 
AAGAAGTCCC TCTCAACAAT GGCCAATATC GATGACCTGC AGGAGACGGA TGTGCTGGCG 
GTGGCTGCCG CGGATGAAGG GGTGCGGCAG CAGCAACAGC AGCAACTCGA GCAGCAACGT 
GAGTCATTAG AGAAACTTGA ACAGGAGTTA TCGACTCTCC GTGATGAAAA TGTGCGACTT 
AAGGAGAATA TTGCAACTCT CACGCAGGAA TTATTAGGGG CTCATGATGA TCTTGAGCTG 
GCAAGAAAAG GTGCTGACTC GAAGACCGCC GAGCTGCGGG GGCAGATTCA GTTATTGGAG 
GCTCAGTTGC GGAACCGCTT GCCCAACACA GATGGAGGAA CCGAGTTGCA CCGTCTTGCG 
GAGGAAAATA GGCAACTTCA ATCGGAGGTG TCTTTACTTT CCGATTCTAT GAATGCGACA 
ACGGGGAATC CTGACGCGAC GGTGCAGCGA CTGAAGGCGG AACTCCAAGC ACTTGCAAAG 
GAGCTTGTGC CGACGAAGAA CAAACTTGTT GAGTACATGG CAATGGCAGA TAGACTGGGC 
ATTCATTATC CCTTTTCACC AGACATGGAG GGGGTTGCTG TCCTTAGGCT TAAAGCAATG 
CATTTTGACA CGGTAACGGG AAGAGTGCGG GCGTGCTCGG CCCCGCGACA ACCATACGCC 
TCGTCTTTTG ACAAAAACGC GTTATCATCA TCGGGTGCCA AGAAGAAAAA AAGAGTGAAG 
AAGCGTCAGC CGCAGGGAAG TCATGTCGAT GAACTTTCAG ATTTGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
Science (New York, N.Y.)309(5733)409-15(2005 Jul)
El-Sayed NM,Myler PJ,Bartholomeu DC,Nilsson D,Aggarwal G,Tran AN,Ghedin E,Worthey EA,Delcher AL,Blandin G,Westenberger SJ,Caler E,Cerqueira GC,Branche C,Haas B,Anupama A,Arner E,Aslund L,Attipoe P,Bontempi E,Bringaud F,Burton P,Cadag E,Campbell DA,Carrington M,Crabtree J,Darban H,da Silveira JF,de Jong P,Edwards K,Englund PT,Fazelina G,Feldblyum T,Ferella M,Frasch AC,Gull K,Horn D,Hou L,Huang Y,Kindlund E,Klingbeil M,Kluge S,Koo H,Lacerda D,Levin MJ,Lorenzi H,Louie T,Machado CR,McCulloch R,McKenna A,Mizuno Y,Mottram JC,Nelson S,Ochaya S,Osoegawa K,Pai G,Parsons M,Pentony M,Pettersson U,Pop M,Ramirez JL,Rinta J,Robertson L,Salzberg SL,Sanchez DO,Seyler A,Sharma R,Shetty J,Simpson AJ,Sisk E,Tammi MT,Tarleton R,Teixeira S,Van Aken S,Vogt C,Ward PN,Wickstead B,Wortman J,White O,Fraser CM,Stuart KD,Andersson B

Comparative genomics of trypanosomatid parasitic protozoa.
Science (New York, N.Y.)309(5733)404-9(2005 Jul)
El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N

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Tc00.1047053503925.70 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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