Tc00.1047053511467.20 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

Gene
Clones
gRNAs

Summary

Gene Symbol	Tc00.1047053511467.20
Entrez Gene ID	3542384
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Trypanosoma cruzi strain CL Brener

mRNA and Protein(s)

mRNA	Protein	Name
XM_806316.1	XP_811409.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Worthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K, Englund PT, Fazelina G, Feldblyum T, Ferella M, Frasch AC, Gull K, Horn D, Hou L, Huang Y, Kindlund E, Klingbeil M, Kluge S, Koo H, Lacerda D, Levin MJ, Lorenzi H, Louie T, Machado CR, McCulloch R, McKenna A, Mizuno Y, Mottram JC, Nelson S, Ochaya S, Osoegawa K, Pai G, Parsons M, Pentony M, Pettersson U, Pop M, Ramirez JL, Rinta J, Robertson L, Salzberg SL, Sanchez DO, Seyler A, Sharma R, Shetty J, Simpson AJ, Sisk E, Tammi MT, Tarleton R, Teixeira S, Van Aken S, Vogt C, Ward PN, Wickstead B, Wortman J, White O, Fraser CM, Stuart KD, Andersson B
Science (New York, N.Y.)309(5733)409-15(2005 Jul)

Comparative genomics of trypanosomatid parasitic protozoa.
El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran AN, Wortman JR, Alsmark UC, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N
Science (New York, N.Y.)309(5733)404-9(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tc00.1047053511467.20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tc00.1047053511467.20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTb06466	XM_806316.1 Latest version!	Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tc00.1047053511467.20 ( XM_806316.1 ) cDNA ORF clone, Trypanosoma cruzi strain CL Brener -> XP_811409.1 Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.

Price & Availability↑

CloneID	OTb06466
Clone ID Related Accession (Same CDS sequence)	XM_806316.1
Accession Version	XM_806316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1225382400000
Organism	Trypanosoma cruzi strain CL Brener
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001849549). COMPLETENESS: incomplete on both ends.

ATGAGCAATG GACCACCCGA CAGGGGTCGG AATTCCCCAG TTGATAATGT CGATATTGAC 
TGGGCGGCTC TTGCTGCACT CCCTTTTGAC GAGTGGGAAC GTACACCAAA CATTCCTGAT 
CTGAATGAGT GGGTTCAGAG AGCTTCCAGA ATAAAATGGA GTGTAGAAGA TGAACATTTT 
TCTCAGCCAC AATATACAAC TGCCATTGTG CGTTTGCTGC GTCTAATTAC AGTGCACTTT 
GTTCACAAGT TAGAACGTCT TCATCAAGAG AAGGAAGAAC TGGAGCGTTC TGCATCTGCT 
GCACGGGATG CTGAGGAAGC TATTAGGGCT CGTGTAGAGC TACTACGGAG GGAAAATAAT 
GACCTTTGTG TATTACTGGA GCAGAAGGGG ATTACTCGAG ACGAGGCAGG GGAAATGTTA 
CCTCTCTATA TTTCGGATTC TGGTGCAGAA AAAAGACGTG TTAAAGAGAA ACTGGAGAAA 
TGTGCAAGGG AATTGGATCG TTTGAAGCAA GAAAACAAGG AACTTCGCCA CATAGTCCGG 
CGTTATAATG AAGAAAAACG CCAGATGGCG AGTGAAGACA GAAAGTTTCG TCTTGAATAC 
GAGCATCTTG CCTCAAAACA CAAGCGGCTC ATGGAAAAGT TTAAGAAGAC TGATGAGATG 
CTGCAGGAAT TTCGAAGTTC AGAAAAAGCT AGGGGGCGAG ATGAAGAAAC TGAACTGCAC 
CAACTGCGTC AGAAGTTGCG GACTCTTCAG CAGGAGAATT ACGATCTAGT TCAGTCACGT 
GACCGTGCCG AGGAGCTTTG TGAAAAGAGG GAAGCAGAAG CGCTGCGGGA CATGAGTGAG 
TTGCAACAGC TTCACCATGA TATCGTGGAG GAGGAAAAGC AACGTTCTCA AGGTTTGGCG 
GAGGAGTTGC AGAAACTGCG ACATGATAGT CAATGTCGTA TAGAAAAGGC CGTTCAGGAA 
CTACAGCAAT CGGTAAAAGA TTTGGAGGAG GAAAATTCTA CACTGCGAAA ACGGTTGGAA 
CGACGTTTTG GGGTTGGACA TCTGTCTGAC CGCGACACCA CGGAAAACAG TGAGAGTGTT 
GGGAAGAATT CGTTGTCTGT CGGTGCAAGC ATGTCTTCGT TTCCTCCAAA CATTTCCCCT 
CATTCCAAAT TTGAGTCGGA CAGTCGCACC AGGCAAAGGT TAATAGCGGA TCGACAGCGA 
CTTCAACAGG AGAATGATCG ACTTGCAGCA GAGTTACAAC AAATGGAAAT TCGAGCTGAA 
TCACGGGATG CGGAAATCAT TAAAATTCAA CGTCTACTGA AGGAATACGA ACGCGGTGAT 
GAAGGCCTTC ATCGCCTACG CGGGGAGTTG GCCGATTCTA ACCGATCCAT AGAACTACTT 
CAAGATGAAA ACGCGCAACT ACATGAGAGG CTTAACGCAA TGGAGGATTC TTTAACATTT 
TCTAATGCCC TTCAGGAATT GTGCATCCGC ATAGGTGTCA CACAAGAAGA AATTGACCGT 
CTTCGTCCAA AAAACACGCC ACTATTTAGT GAAGTGGAAA CGCTACGAGA AGAGGTGGCC 
ACATTAAAGG ATGAAGTAGT GTGGCTGGAA AAGGAGCGCC GACATTGGAT GGACAAGGTT 
CGTTTGAAGC CGTTGCTTGA CACGAAGCTT CGACTCAAGT TGGGCCTTTC CACAGAACAA 
CTGAGGCAGC TAGACCAGAT GGTGGATCAG ATGAAGTCAG GAAGACTGAT CATCGAAGAG 
GACGCAGAGG ATAACTACAA GGAGAAGTAT TTCCAAGAAC TGCAGCTTCG GCGAAAAGAG 
ATGGAACGTT TTAACGCTTT TGTCCGTCAT CGAATTGAGG AGGTTTTGCG AGAAGTGCTT 
GGAATAACGG ATTTAAAATC TGAATCAGAA GCAAAGTCGG CGTTAAATGC TCTGCGGGAG 
CGTTTTGACT TTATCACCAC CCTTCCACCA ATGGGCTCGA CAGAAGAGCC ACCATTAGAT 
GCCGCGCAGT TGCGTATGCA GCTTCAAAGC GCAGCACAAC TTTTGGAGCA GTCAGAGCTC 
ACTATCAAGG AACATGCGGC TTCTCAGGCG GTGCTGTGCG AACAACTGGC AGCTGTGACG 
GCAGAGCGGG ACATGCTGAT AGGCGAACGT GATCAATACC GTGCCGCTGT TTTTGAATCA 
CTAGGGACCA CACCGTATGC CGTAACAAAA GCTCAGCGGC ACGAGTCAAA TGTGGGAAAA 
ATGGAGGGCC CTTCTTCTTT GGCAGCTCCC GTTTTTGGGG GTGCCAGCGA TGCCCCTTGT 
TTACCGTCAT TGCCTGCTGT GCAGAAGGAG AATTTGGCTT CCCCGAGATC TCCCTTTTTC 
GCTTCCCTTT TGCAAACCTT TGAGGAACAA CTTCGGGTGA AGGATGAGCT TATTGCCTCT 
CTTAAAGGGA CCGTTGAGGC GGCAAAAAAT GGGGCGGCGC AGCATCGTGA GGCAAAAATA 
AAAGCAACGG AGCAACTCTC AGAGGCGTGC GCGGCGCAGG AGGACCTCCG GAATCAACTA 
TTGGCGATCC AGCAAATGAA TGAGGAGCTC CGCGGCAAAT TGGATGAAAA AAACAAGATT 
GTAGAAGATC TGCAAGAAGC GATGCAGCGT CTTGAATCGG ACAATACACG CCAAATTTTT 
CAAAAGATTG TTGTGCTAAG ACAACGTGAG GGAAAATTAT TAGAGCGTTT GCGTCGTGCA 
AGAGAAACTC AAGAAGAGGC GTTGAGGTCA GAGCGAGCGA TGCGGGAATA CGTCGATACC 
ACCTTTAAGT CTCTTAAGGA GGCGTTAGAT AATACGTCTA CAGGATTTGT TCTCCCGCGT 
TCTTCCGGTG TTATTTGCGT AGAGAAAGAT GTTCTTGAGG AAGTCCAACA GCGGTTTGAA 
GGGGCGTTAC GAGGAAAATT CTTCAAGGAG GACTCTGCAT ACCTTTTGCA GCTTCATGAA 
ATCTATCGTA ACATGGAACA AATGGAAGAA CTGAACATCT TACGAGTCCG TGAAAAAGAT 
GGGGAAAAAA AAATTGAAGA GATGAAAATT GAGATGAATG AATTGCGAGC TGAATTGGAG 
TGTCTCCGGA AAGCGCATGA AGAATCCCCC GACACGCACC GGAATGAAGC TTCGGAGGCC 
GCAAGATGGG AAACAGAGGC CACAACATTA CGTCAAAAGT GTACGCTTTA CATGAGACGT 
TGCGAGGACA AGGAACGAGA GGTGTCTACA CTCGAAGCTG AACTTGGGGA GGCCCGGGAA 
GAGTTGGCGT TTTTGCAGGA ACACATACAT AATCTCAGTT GTGGCAGTCG GAACGACAAC 
ACAACGGGTG TCACGCGGAA GCTGGGTGTT GTCCTTCAGG AGCAAAAAGA AGAAATGTCA 
CGACAACAAC AAAAACAAGA TGAAGAAGAA CTGCAAATAA AGCAGCAGTG TGAAACGAAG 
CAAATTTGTC ACGGGACCAC TGTGGATAAC AAGGTGCGGC AACTAGAACG CGATGTGGCT 
CGTCTGAAGT CCATCAATTT GGGTCTTTTG CATCACTCTC TCGATTTACA AGGAGAATTC 
AAGCGTCTGG AGATTCAACT AGAGGCCACA AAGCAAGAAC TCTCGCTCGT ACGAGACGCG 
GGAGATTCCA GGAAAATAAG CGACTTTGTA TCTGCTGCTA TTCAGCAACA TGCAGCGTTA 
CGTAGGCAAA GTGAGTTATC GTTGCTTCGG GCAAAACGGA CCCGAATGCA ACTTTATGCC 
TCCGAGGCAA ATCTGCGTGT GGCGGTGAAT GAGGCTACCT CGTACAGGCT TAGTGCGTTT 
CGTCTCTACC GGACCTATGT TGCACAGATG GTGTCTGTTT TGGACTATGT TCGTGGCCTG 
CAACGAGGAG TGAAGGGTAG TATTTCTCCG CATCGTGTTG CAATGATGCA CAAACGTTTT 
GTTGACGCTA TTGCAGATGT TGAGCGCGGC CAGGCCAGAC AAATTGAGAT GGCTTCAAAG 
CTCGCTGAGA GTGACGGAAT GGTAAACCTT TTGCAGCAGC AATTAGATCT TCTCAAAGCA 
AAGGACTTTG AGGAGCGCCA AGATGCACTT CACGCCAAAC TGTTGACTTC CCTTTCTGCT 
GTTCGCGAAA AAGACATGCG GCTTGTGGAG CTACAGGAGG ATCATCAGTA CATGCAACAA 
AAATTGAAAA GGGCTGAGTC GCACATACAA AATTTGACCG AGGAATTGGC TCGTTTAGAG 
TTGCGGGCCA GTGGTGGTGT CTCACTAAAT GAAGACATAC TTCAGAACCT CTTGCAATTA 
AAGGAAACTG TCTTTGCAAA GGTGGAATCG CCGGCACTCA TTATGCAATC CAGTGGAGGT 
GGCTTATGTG ACATGGATGA CGGGGGTGCA GACACCGCAA TTCAGGAGTA CAAACTGGCA 
CTTGACAAAC AGGCGGAATT AAAACGGGAA TTAAACTTGC TTAGGAAGCG ATGGGAAAAC 
GAAGTTTCCG AAAGCAAGAA GGCGCGTACC GAGACAGAGT CCCTAAAGGA GGAAATGAAT 
CACCTCAGGG GACGTCTGCA GTACGCGCAA CGACAATTGG AGGAAGAACG ACAAAAGGGA 
GAGGAGCGCG AGCGGCGAAT TATACGATCA CATGAAGCAC AAGTAGAGGT AACACGTCGT 
GCAGCAGAAC ACAACAGCCA GTGTCTTCGG GACATGTTAC AGAATAAAGA AGCATGCATC 
AAACAGCTTC AAGAACAGCT TCAATCAGAG CGAAGAAAGT ATCTTGAGTA TCAGCTTGAA 
GAATCTTCAC GCATGGAGCG ATTACATGAT CACCTCTTCA AGGAGAATAG CGCCATGATG 
GAGCGTTTCC GCGAGGCCAT TGACGGTGTG ACGGAGAACT ACACGTATAA CACAACGGCA 
CAAGGGGCGA CTGTGTGTGA CGCATCACCG GATGGAGTGG CAGCACAACT TGCACTGCTT 
ACGAAAGAGA CATTACGTCT AAAGGCGGAA TTAAAGGATG CACGCATGAC AAACATTATG 
CTTGAGGCCC AACTGAACGA ACAGGTGACA AAGTCACAGA ATCAGCTGCT GCAGCAGCAG 
CAAGGACCCA ATTACAGCCC TCAGCAGGCG CCACAGAAAG AAGCAACAGC TGAGGCTTCT 
GTGGTTGGCG TTATTAAGGA TCAGAATGCT ATAATAGAGA GTTTGCGTCA ACGCGAATTT 
TCTCTTACCC GTGAAATGCA ACGATACAGA AATGAGAGGG ATTTGTTGGA GCAGCAGCTA 
CATGAGGTTC GCCATACTAT TGTGGAGCAG GGTGGGGTCC TGAAGAGTGT CGCAACTTTG 
GAAATGACAG GTTCGTCGGT GGAACAAGAA TTACGGGCGC AGTTGATTTT TGTGGAGAGT 
CAACTTGTGG AGACGCGGGC GCAGTTGGAA GAAGAGCGGC AAAGTGCGCG CCGACTGCAG 
GCGGATACCA GTGGATGGCG ATCTCATCTG GATGCCTTAC GCGAGGAAGT TGTAAGGCAA 
CAAGCTGACG TTGAGCGAGC TCGGCACCTT GTCGCTATGA ATGAAGCTCT TAACGTCGAT 
ATGCGGCGAA TGGAGGAGCA GAATGAGAAG CTTATCCTGG CAACCAAAAT GCTAAAGGAG 
AAACTGATTG AGCAGGCACA ACAACGTGGT GATGATTCAC GCAAACGACA ACATGAAATT 
GCGTTGGCGC AGCGGATGGG TTCCATTCAG TTGGAATCCA CCGAAAAGTT GAGGACCGTG 
AACGAACGGC TACACTCGAT CCAGCAGGAG CTCGAGGAGA AAGTCCGTCG AGAAGAGGAG 
GCTCTTAAGA AGCATGAGGA GGCACAGCGA TTGGCGTATG ATTTGCACCG ACAGCTTCAG 
GAGCGAGAAG AAGAAATTTT GCGTCTTAAG CGCGAGTTGA GCGGCACCCA CCCCGCGTCC 
CTCCATGCGG TGCGAGGCAA GCATGAAAAG GGAAGGCAAC TCCTGTATAC CGTGAAAGAG 
GAGAAACAGG ACACGTATGA GAGGGAAGAA AAAATGCCAA GAAATTTCTT GAAAGTTGAA 
AGTGCAGAGA AGCGAGAGGA AGAAAAGGTG CAAAAGCGTC CAGCACACCG TTCAACTTCT 
TCTTCCTCCG CTCCCATTCG TGCATTGCTT GCGGACGCGA ACGCAAACGT TATTGTCAAA 
CCGCAGATAG CAGCAATGCT ACAGCGAGAG ATTGAAAAGG CGCAGAGAAA CAACATGCAT 
GAAATATCAT CGTTGCGGGC GAATGTACGC CGTCTTGAAA GCGATCTGGA AGAGGCTCGT 
CAACAACTAC GAGGAGAGCG CGATAACAGT CGCTCTCTGC GTGTCATGAT TCAAAATGCC 
CGACGTGAAC TTGAGGAAAA GGAGCTTGCT CTGGCACGAG CGTCTGCCAG TCGGCAGCAA 
CGTGCCAAGG AGCATCAGCA TGTTCAAGGC AATTTTGTAA CGGTTTCGAG CAAAACTCCT 
GTGACAGATT CTACCGCGGC CGTTTCCCCC GTGCCAAATA CAGCCGTATC AGGTGAACAA 
GATCAGCTGC AGCGTCAACG GGAAGAAAAT GAACAACTTC GACGCCAAGT TGAACGACTC 
AAGAAGCGTG TGGTGACCTT TGACAATGTC GTTAGTGAAG CGGAATCCTA CAAGAAGGAG 
TTGGCAGATC TTCGGTCTCA GCATCCGGAG TCTGTGGATT CAGCTTATAT GGCCTCCCCA 
CTTGATATAC GTTCCCACAA ACGGATTGTT CTCCATTTAG AGAGTGTCAT TGACTCGCTT 
CGAAGAGAGC TAAGCGTCAA GAAGGAGATG CAGATGCGCA ACTTGCAAAG ACGTATCGAT 
GAGTTGACAG TGGAGAACCA ACGTCTGGCG GCGGAATTGA GCAGTCGCTC TCAATTCACG 
CCAAATTTGC AGCCCCCTAT AGCCCCGTCT AGGGCCGATA ACGGAAGTGA GCGGGCAACT 
CTGCAGCGGG AATTATTGGA AAAGAACGGC ATTATTCTTG ATCTCCGCTT TGAGCGTGAG 
GCGCTGCAAC TTAAGGTTGG ACGTCTGGAG CGACACATTG AGGAAATAAT TCAGGTAGAC 
TCAACCAACA CAAAACGCAC GGGAACTTCA CAGCAACGGA GTCGCGCCGA GGCGTTGGAG 
TCTTTGGTTG AAAATCTGAA GCTTGTTGTT GAGCGTCTTC AGAAGGAGAA CAATACGCTG 
AAGTCGAAGA CTGTAAGTAT GTCAAAGCAC ATGGATCTTG TGCGAGAGCT TCGTGAACTC 
CGGCGAAGTG AACAAAAACT CCGCGAGCAT TCGGAGATGT TGACAAAGCG CCTCTTAGAA 
TCCGCACCTA GCGGAAGCGC CATGTCAAAA CAACAAGTGA CACTCCAGCG GAGGCTGCAG 
ACGGCCCAAG CGACCATGGA GCAGTATGAG GCGGAAATGC TTGAGCTAAA ACAAAAGCTT 
GATGATCGCC AGCACGCAGT GGAAGGGGAG GAGTTCATGC CGTCTGGGCA AAGGCATCAG 
GAACAGTTGC CAGAGTGGGA AAATCGCCGC TTGGTGCAGC CAATTGAGAC ATGGTCTCAA 
GACCTGCCGC CGCCTCTGCC CAGCCCGCCG GTGACACTCA CCGGGGAAGA TCCCTTTGGT 
TATCTGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_811409.1
CDS	1..7572
Translation	MSNGPPDRGRNSPVDNVDIDWAALAALPFDEWERTPNIPDLNEWVQRASRIKWSVEDEHFSQPQYTTAIVRLLRLITVHFVHKLERLHQEKEELERSASAARDAEEAIRARVELLRRENNDLCVLLEQKGITRDEAGEMLPLYISDSGAEKRRVKEKLEKCARELDRLKQENKELRHIVRRYNEEKRQMASEDRKFRLEYEHLASKHKRLMEKFKKTDEMLQEFRSSEKARGRDEETELHQLRQKLRTLQQENYDLVQSRDRAEELCEKREAEALRDMSELQQLHHDIVEEEKQRSQGLAEELQKLRHDSQCRIEKAVQELQQSVKDLEEENSTLRKRLERRFGVGHLSDRDTTENSESVGKNSLSVGASMSSFPPNISPHSKFESDSRTRQRLIADRQRLQQENDRLAAELQQMEIRAESRDAEIIKIQRLLKEYERGDEGLHRLRGELADSNRSIELLQDENAQLHERLNAMEDSLTFSNALQELCIRIGVTQEEIDRLRPKNTPLFSEVETLREEVATLKDEVVWLEKERRHWMDKVRLKPLLDTKLRLKLGLSTEQLRQLDQMVDQMKSGRLIIEEDAEDNYKEKYFQELQLRRKEMERFNAFVRHRIEEVLREVLGITDLKSESEAKSALNALRERFDFITTLPPMGSTEEPPLDAAQLRMQLQSAAQLLEQSELTIKEHAASQAVLCEQLAAVTAERDMLIGERDQYRAAVFESLGTTPYAVTKAQRHESNVGKMEGPSSLAAPVFGGASDAPCLPSLPAVQKENLASPRSPFFASLLQTFEEQLRVKDELIASLKGTVEAAKNGAAQHREAKIKATEQLSEACAAQEDLRNQLLAIQQMNEELRGKLDEKNKIVEDLQEAMQRLESDNTRQIFQKIVVLRQREGKLLERLRRARETQEEALRSERAMREYVDTTFKSLKEALDNTSTGFVLPRSSGVICVEKDVLEEVQQRFEGALRGKFFKEDSAYLLQLHEIYRNMEQMEELNILRVREKDGEKKIEEMKIEMNELRAELECLRKAHEESPDTHRNEASEAARWETEATTLRQKCTLYMRRCEDKEREVSTLEAELGEAREELAFLQEHIHNLSCGSRNDNTTGVTRKLGVVLQEQKEEMSRQQQKQDEEELQIKQQCETKQICHGTTVDNKVRQLERDVARLKSINLGLLHHSLDLQGEFKRLEIQLEATKQELSLVRDAGDSRKISDFVSAAIQQHAALRRQSELSLLRAKRTRMQLYASEANLRVAVNEATSYRLSAFRLYRTYVAQMVSVLDYVRGLQRGVKGSISPHRVAMMHKRFVDAIADVERGQARQIEMASKLAESDGMVNLLQQQLDLLKAKDFEERQDALHAKLLTSLSAVREKDMRLVELQEDHQYMQQKLKRAESHIQNLTEELARLELRASGGVSLNEDILQNLLQLKETVFAKVESPALIMQSSGGGLCDMDDGGADTAIQEYKLALDKQAELKRELNLLRKRWENEVSESKKARTETESLKEEMNHLRGRLQYAQRQLEEERQKGEERERRIIRSHEAQVEVTRRAAEHNSQCLRDMLQNKEACIKQLQEQLQSERRKYLEYQLEESSRMERLHDHLFKENSAMMERFREAIDGVTENYTYNTTAQGATVCDASPDGVAAQLALLTKETLRLKAELKDARMTNIMLEAQLNEQVTKSQNQLLQQQQGPNYSPQQAPQKEATAEASVVGVIKDQNAIIESLRQREFSLTREMQRYRNERDLLEQQLHEVRHTIVEQGGVLKSVATLEMTGSSVEQELRAQLIFVESQLVETRAQLEEERQSARRLQADTSGWRSHLDALREEVVRQQADVERARHLVAMNEALNVDMRRMEEQNEKLILATKMLKEKLIEQAQQRGDDSRKRQHEIALAQRMGSIQLESTEKLRTVNERLHSIQQELEEKVRREEEALKKHEEAQRLAYDLHRQLQEREEEILRLKRELSGTHPASLHAVRGKHEKGRQLLYTVKEEKQDTYEREEKMPRNFLKVESAEKREEEKVQKRPAHRSTSSSSAPIRALLADANANVIVKPQIAAMLQREIEKAQRNNMHEISSLRANVRRLESDLEEARQQLRGERDNSRSLRVMIQNARRELEEKELALARASASRQQRAKEHQHVQGNFVTVSSKTPVTDSTAAVSPVPNTAVSGEQDQLQRQREENEQLRRQVERLKKRVVTFDNVVSEAESYKKELADLRSQHPESVDSAYMASPLDIRSHKRIVLHLESVIDSLRRELSVKKEMQMRNLQRRIDELTVENQRLAAELSSRSQFTPNLQPPIAPSRADNGSERATLQRELLEKNGIILDLRFEREALQLKVGRLERHIEEIIQVDSTNTKRTGTSQQRSRAEALESLVENLKLVVERLQKENNTLKSKTVSMSKHMDLVRELRELRRSEQKLREHSEMLTKRLLESAPSGSAMSKQQVTLQRRLQTAQATMEQYEAEMLELKQKLDDRQHAVEGEEFMPSGQRHQEQLPEWENRRLVQPIETWSQDLPPPLPSPPVTLTGEDPFGYLT

Target ORF information:

RefSeq Version	XM_806316.1
Organism	Trypanosoma cruzi strain CL Brener
Definition	Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_806316.1

ORF Insert Sequence:

ATGAGCAATG GACCACCCGA CAGGGGTCGG AATTCCCCAG TTGATAATGT CGATATTGAC 
TGGGCGGCTC TTGCTGCACT CCCTTTTGAC GAGTGGGAAC GTACACCAAA CATTCCTGAT 
CTGAATGAGT GGGTTCAGAG AGCTTCCAGA ATAAAATGGA GTGTAGAAGA TGAACATTTT 
TCTCAGCCAC AATATACAAC TGCCATTGTG CGTTTGCTGC GTCTAATTAC AGTGCACTTT 
GTTCACAAGT TAGAACGTCT TCATCAAGAG AAGGAAGAAC TGGAGCGTTC TGCATCTGCT 
GCACGGGATG CTGAGGAAGC TATTAGGGCT CGTGTAGAGC TACTACGGAG GGAAAATAAT 
GACCTTTGTG TATTACTGGA GCAGAAGGGG ATTACTCGAG ACGAGGCAGG GGAAATGTTA 
CCTCTCTATA TTTCGGATTC TGGTGCAGAA AAAAGACGTG TTAAAGAGAA ACTGGAGAAA 
TGTGCAAGGG AATTGGATCG TTTGAAGCAA GAAAACAAGG AACTTCGCCA CATAGTCCGG 
CGTTATAATG AAGAAAAACG CCAGATGGCG AGTGAAGACA GAAAGTTTCG TCTTGAATAC 
GAGCATCTTG CCTCAAAACA CAAGCGGCTC ATGGAAAAGT TTAAGAAGAC TGATGAGATG 
CTGCAGGAAT TTCGAAGTTC AGAAAAAGCT AGGGGGCGAG ATGAAGAAAC TGAACTGCAC 
CAACTGCGTC AGAAGTTGCG GACTCTTCAG CAGGAGAATT ACGATCTAGT TCAGTCACGT 
GACCGTGCCG AGGAGCTTTG TGAAAAGAGG GAAGCAGAAG CGCTGCGGGA CATGAGTGAG 
TTGCAACAGC TTCACCATGA TATCGTGGAG GAGGAAAAGC AACGTTCTCA AGGTTTGGCG 
GAGGAGTTGC AGAAACTGCG ACATGATAGT CAATGTCGTA TAGAAAAGGC CGTTCAGGAA 
CTACAGCAAT CGGTAAAAGA TTTGGAGGAG GAAAATTCTA CACTGCGAAA ACGGTTGGAA 
CGACGTTTTG GGGTTGGACA TCTGTCTGAC CGCGACACCA CGGAAAACAG TGAGAGTGTT 
GGGAAGAATT CGTTGTCTGT CGGTGCAAGC ATGTCTTCGT TTCCTCCAAA CATTTCCCCT 
CATTCCAAAT TTGAGTCGGA CAGTCGCACC AGGCAAAGGT TAATAGCGGA TCGACAGCGA 
CTTCAACAGG AGAATGATCG ACTTGCAGCA GAGTTACAAC AAATGGAAAT TCGAGCTGAA 
TCACGGGATG CGGAAATCAT TAAAATTCAA CGTCTACTGA AGGAATACGA ACGCGGTGAT 
GAAGGCCTTC ATCGCCTACG CGGGGAGTTG GCCGATTCTA ACCGATCCAT AGAACTACTT 
CAAGATGAAA ACGCGCAACT ACATGAGAGG CTTAACGCAA TGGAGGATTC TTTAACATTT 
TCTAATGCCC TTCAGGAATT GTGCATCCGC ATAGGTGTCA CACAAGAAGA AATTGACCGT 
CTTCGTCCAA AAAACACGCC ACTATTTAGT GAAGTGGAAA CGCTACGAGA AGAGGTGGCC 
ACATTAAAGG ATGAAGTAGT GTGGCTGGAA AAGGAGCGCC GACATTGGAT GGACAAGGTT 
CGTTTGAAGC CGTTGCTTGA CACGAAGCTT CGACTCAAGT TGGGCCTTTC CACAGAACAA 
CTGAGGCAGC TAGACCAGAT GGTGGATCAG ATGAAGTCAG GAAGACTGAT CATCGAAGAG 
GACGCAGAGG ATAACTACAA GGAGAAGTAT TTCCAAGAAC TGCAGCTTCG GCGAAAAGAG 
ATGGAACGTT TTAACGCTTT TGTCCGTCAT CGAATTGAGG AGGTTTTGCG AGAAGTGCTT 
GGAATAACGG ATTTAAAATC TGAATCAGAA GCAAAGTCGG CGTTAAATGC TCTGCGGGAG 
CGTTTTGACT TTATCACCAC CCTTCCACCA ATGGGCTCGA CAGAAGAGCC ACCATTAGAT 
GCCGCGCAGT TGCGTATGCA GCTTCAAAGC GCAGCACAAC TTTTGGAGCA GTCAGAGCTC 
ACTATCAAGG AACATGCGGC TTCTCAGGCG GTGCTGTGCG AACAACTGGC AGCTGTGACG 
GCAGAGCGGG ACATGCTGAT AGGCGAACGT GATCAATACC GTGCCGCTGT TTTTGAATCA 
CTAGGGACCA CACCGTATGC CGTAACAAAA GCTCAGCGGC ACGAGTCAAA TGTGGGAAAA 
ATGGAGGGCC CTTCTTCTTT GGCAGCTCCC GTTTTTGGGG GTGCCAGCGA TGCCCCTTGT 
TTACCGTCAT TGCCTGCTGT GCAGAAGGAG AATTTGGCTT CCCCGAGATC TCCCTTTTTC 
GCTTCCCTTT TGCAAACCTT TGAGGAACAA CTTCGGGTGA AGGATGAGCT TATTGCCTCT 
CTTAAAGGGA CCGTTGAGGC GGCAAAAAAT GGGGCGGCGC AGCATCGTGA GGCAAAAATA 
AAAGCAACGG AGCAACTCTC AGAGGCGTGC GCGGCGCAGG AGGACCTCCG GAATCAACTA 
TTGGCGATCC AGCAAATGAA TGAGGAGCTC CGCGGCAAAT TGGATGAAAA AAACAAGATT 
GTAGAAGATC TGCAAGAAGC GATGCAGCGT CTTGAATCGG ACAATACACG CCAAATTTTT 
CAAAAGATTG TTGTGCTAAG ACAACGTGAG GGAAAATTAT TAGAGCGTTT GCGTCGTGCA 
AGAGAAACTC AAGAAGAGGC GTTGAGGTCA GAGCGAGCGA TGCGGGAATA CGTCGATACC 
ACCTTTAAGT CTCTTAAGGA GGCGTTAGAT AATACGTCTA CAGGATTTGT TCTCCCGCGT 
TCTTCCGGTG TTATTTGCGT AGAGAAAGAT GTTCTTGAGG AAGTCCAACA GCGGTTTGAA 
GGGGCGTTAC GAGGAAAATT CTTCAAGGAG GACTCTGCAT ACCTTTTGCA GCTTCATGAA 
ATCTATCGTA ACATGGAACA AATGGAAGAA CTGAACATCT TACGAGTCCG TGAAAAAGAT 
GGGGAAAAAA AAATTGAAGA GATGAAAATT GAGATGAATG AATTGCGAGC TGAATTGGAG 
TGTCTCCGGA AAGCGCATGA AGAATCCCCC GACACGCACC GGAATGAAGC TTCGGAGGCC 
GCAAGATGGG AAACAGAGGC CACAACATTA CGTCAAAAGT GTACGCTTTA CATGAGACGT 
TGCGAGGACA AGGAACGAGA GGTGTCTACA CTCGAAGCTG AACTTGGGGA GGCCCGGGAA 
GAGTTGGCGT TTTTGCAGGA ACACATACAT AATCTCAGTT GTGGCAGTCG GAACGACAAC 
ACAACGGGTG TCACGCGGAA GCTGGGTGTT GTCCTTCAGG AGCAAAAAGA AGAAATGTCA 
CGACAACAAC AAAAACAAGA TGAAGAAGAA CTGCAAATAA AGCAGCAGTG TGAAACGAAG 
CAAATTTGTC ACGGGACCAC TGTGGATAAC AAGGTGCGGC AACTAGAACG CGATGTGGCT 
CGTCTGAAGT CCATCAATTT GGGTCTTTTG CATCACTCTC TCGATTTACA AGGAGAATTC 
AAGCGTCTGG AGATTCAACT AGAGGCCACA AAGCAAGAAC TCTCGCTCGT ACGAGACGCG 
GGAGATTCCA GGAAAATAAG CGACTTTGTA TCTGCTGCTA TTCAGCAACA TGCAGCGTTA 
CGTAGGCAAA GTGAGTTATC GTTGCTTCGG GCAAAACGGA CCCGAATGCA ACTTTATGCC 
TCCGAGGCAA ATCTGCGTGT GGCGGTGAAT GAGGCTACCT CGTACAGGCT TAGTGCGTTT 
CGTCTCTACC GGACCTATGT TGCACAGATG GTGTCTGTTT TGGACTATGT TCGTGGCCTG 
CAACGAGGAG TGAAGGGTAG TATTTCTCCG CATCGTGTTG CAATGATGCA CAAACGTTTT 
GTTGACGCTA TTGCAGATGT TGAGCGCGGC CAGGCCAGAC AAATTGAGAT GGCTTCAAAG 
CTCGCTGAGA GTGACGGAAT GGTAAACCTT TTGCAGCAGC AATTAGATCT TCTCAAAGCA 
AAGGACTTTG AGGAGCGCCA AGATGCACTT CACGCCAAAC TGTTGACTTC CCTTTCTGCT 
GTTCGCGAAA AAGACATGCG GCTTGTGGAG CTACAGGAGG ATCATCAGTA CATGCAACAA 
AAATTGAAAA GGGCTGAGTC GCACATACAA AATTTGACCG AGGAATTGGC TCGTTTAGAG 
TTGCGGGCCA GTGGTGGTGT CTCACTAAAT GAAGACATAC TTCAGAACCT CTTGCAATTA 
AAGGAAACTG TCTTTGCAAA GGTGGAATCG CCGGCACTCA TTATGCAATC CAGTGGAGGT 
GGCTTATGTG ACATGGATGA CGGGGGTGCA GACACCGCAA TTCAGGAGTA CAAACTGGCA 
CTTGACAAAC AGGCGGAATT AAAACGGGAA TTAAACTTGC TTAGGAAGCG ATGGGAAAAC 
GAAGTTTCCG AAAGCAAGAA GGCGCGTACC GAGACAGAGT CCCTAAAGGA GGAAATGAAT 
CACCTCAGGG GACGTCTGCA GTACGCGCAA CGACAATTGG AGGAAGAACG ACAAAAGGGA 
GAGGAGCGCG AGCGGCGAAT TATACGATCA CATGAAGCAC AAGTAGAGGT AACACGTCGT 
GCAGCAGAAC ACAACAGCCA GTGTCTTCGG GACATGTTAC AGAATAAAGA AGCATGCATC 
AAACAGCTTC AAGAACAGCT TCAATCAGAG CGAAGAAAGT ATCTTGAGTA TCAGCTTGAA 
GAATCTTCAC GCATGGAGCG ATTACATGAT CACCTCTTCA AGGAGAATAG CGCCATGATG 
GAGCGTTTCC GCGAGGCCAT TGACGGTGTG ACGGAGAACT ACACGTATAA CACAACGGCA 
CAAGGGGCGA CTGTGTGTGA CGCATCACCG GATGGAGTGG CAGCACAACT TGCACTGCTT 
ACGAAAGAGA CATTACGTCT AAAGGCGGAA TTAAAGGATG CACGCATGAC AAACATTATG 
CTTGAGGCCC AACTGAACGA ACAGGTGACA AAGTCACAGA ATCAGCTGCT GCAGCAGCAG 
CAAGGACCCA ATTACAGCCC TCAGCAGGCG CCACAGAAAG AAGCAACAGC TGAGGCTTCT 
GTGGTTGGCG TTATTAAGGA TCAGAATGCT ATAATAGAGA GTTTGCGTCA ACGCGAATTT 
TCTCTTACCC GTGAAATGCA ACGATACAGA AATGAGAGGG ATTTGTTGGA GCAGCAGCTA 
CATGAGGTTC GCCATACTAT TGTGGAGCAG GGTGGGGTCC TGAAGAGTGT CGCAACTTTG 
GAAATGACAG GTTCGTCGGT GGAACAAGAA TTACGGGCGC AGTTGATTTT TGTGGAGAGT 
CAACTTGTGG AGACGCGGGC GCAGTTGGAA GAAGAGCGGC AAAGTGCGCG CCGACTGCAG 
GCGGATACCA GTGGATGGCG ATCTCATCTG GATGCCTTAC GCGAGGAAGT TGTAAGGCAA 
CAAGCTGACG TTGAGCGAGC TCGGCACCTT GTCGCTATGA ATGAAGCTCT TAACGTCGAT 
ATGCGGCGAA TGGAGGAGCA GAATGAGAAG CTTATCCTGG CAACCAAAAT GCTAAAGGAG 
AAACTGATTG AGCAGGCACA ACAACGTGGT GATGATTCAC GCAAACGACA ACATGAAATT 
GCGTTGGCGC AGCGGATGGG TTCCATTCAG TTGGAATCCA CCGAAAAGTT GAGGACCGTG 
AACGAACGGC TACACTCGAT CCAGCAGGAG CTCGAGGAGA AAGTCCGTCG AGAAGAGGAG 
GCTCTTAAGA AGCATGAGGA GGCACAGCGA TTGGCGTATG ATTTGCACCG ACAGCTTCAG 
GAGCGAGAAG AAGAAATTTT GCGTCTTAAG CGCGAGTTGA GCGGCACCCA CCCCGCGTCC 
CTCCATGCGG TGCGAGGCAA GCATGAAAAG GGAAGGCAAC TCCTGTATAC CGTGAAAGAG 
GAGAAACAGG ACACGTATGA GAGGGAAGAA AAAATGCCAA GAAATTTCTT GAAAGTTGAA 
AGTGCAGAGA AGCGAGAGGA AGAAAAGGTG CAAAAGCGTC CAGCACACCG TTCAACTTCT 
TCTTCCTCCG CTCCCATTCG TGCATTGCTT GCGGACGCGA ACGCAAACGT TATTGTCAAA 
CCGCAGATAG CAGCAATGCT ACAGCGAGAG ATTGAAAAGG CGCAGAGAAA CAACATGCAT 
GAAATATCAT CGTTGCGGGC GAATGTACGC CGTCTTGAAA GCGATCTGGA AGAGGCTCGT 
CAACAACTAC GAGGAGAGCG CGATAACAGT CGCTCTCTGC GTGTCATGAT TCAAAATGCC 
CGACGTGAAC TTGAGGAAAA GGAGCTTGCT CTGGCACGAG CGTCTGCCAG TCGGCAGCAA 
CGTGCCAAGG AGCATCAGCA TGTTCAAGGC AATTTTGTAA CGGTTTCGAG CAAAACTCCT 
GTGACAGATT CTACCGCGGC CGTTTCCCCC GTGCCAAATA CAGCCGTATC AGGTGAACAA 
GATCAGCTGC AGCGTCAACG GGAAGAAAAT GAACAACTTC GACGCCAAGT TGAACGACTC 
AAGAAGCGTG TGGTGACCTT TGACAATGTC GTTAGTGAAG CGGAATCCTA CAAGAAGGAG 
TTGGCAGATC TTCGGTCTCA GCATCCGGAG TCTGTGGATT CAGCTTATAT GGCCTCCCCA 
CTTGATATAC GTTCCCACAA ACGGATTGTT CTCCATTTAG AGAGTGTCAT TGACTCGCTT 
CGAAGAGAGC TAAGCGTCAA GAAGGAGATG CAGATGCGCA ACTTGCAAAG ACGTATCGAT 
GAGTTGACAG TGGAGAACCA ACGTCTGGCG GCGGAATTGA GCAGTCGCTC TCAATTCACG 
CCAAATTTGC AGCCCCCTAT AGCCCCGTCT AGGGCCGATA ACGGAAGTGA GCGGGCAACT 
CTGCAGCGGG AATTATTGGA AAAGAACGGC ATTATTCTTG ATCTCCGCTT TGAGCGTGAG 
GCGCTGCAAC TTAAGGTTGG ACGTCTGGAG CGACACATTG AGGAAATAAT TCAGGTAGAC 
TCAACCAACA CAAAACGCAC GGGAACTTCA CAGCAACGGA GTCGCGCCGA GGCGTTGGAG 
TCTTTGGTTG AAAATCTGAA GCTTGTTGTT GAGCGTCTTC AGAAGGAGAA CAATACGCTG 
AAGTCGAAGA CTGTAAGTAT GTCAAAGCAC ATGGATCTTG TGCGAGAGCT TCGTGAACTC 
CGGCGAAGTG AACAAAAACT CCGCGAGCAT TCGGAGATGT TGACAAAGCG CCTCTTAGAA 
TCCGCACCTA GCGGAAGCGC CATGTCAAAA CAACAAGTGA CACTCCAGCG GAGGCTGCAG 
ACGGCCCAAG CGACCATGGA GCAGTATGAG GCGGAAATGC TTGAGCTAAA ACAAAAGCTT 
GATGATCGCC AGCACGCAGT GGAAGGGGAG GAGTTCATGC CGTCTGGGCA AAGGCATCAG 
GAACAGTTGC CAGAGTGGGA AAATCGCCGC TTGGTGCAGC CAATTGAGAC ATGGTCTCAA 
GACCTGCCGC CGCCTCTGCC CAGCCCGCCG GTGACACTCA CCGGGGAAGA TCCCTTTGGT 
TATCTGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
Science (New York, N.Y.)309(5733)409-15(2005 Jul)
El-Sayed NM,Myler PJ,Bartholomeu DC,Nilsson D,Aggarwal G,Tran AN,Ghedin E,Worthey EA,Delcher AL,Blandin G,Westenberger SJ,Caler E,Cerqueira GC,Branche C,Haas B,Anupama A,Arner E,Aslund L,Attipoe P,Bontempi E,Bringaud F,Burton P,Cadag E,Campbell DA,Carrington M,Crabtree J,Darban H,da Silveira JF,de Jong P,Edwards K,Englund PT,Fazelina G,Feldblyum T,Ferella M,Frasch AC,Gull K,Horn D,Hou L,Huang Y,Kindlund E,Klingbeil M,Kluge S,Koo H,Lacerda D,Levin MJ,Lorenzi H,Louie T,Machado CR,McCulloch R,McKenna A,Mizuno Y,Mottram JC,Nelson S,Ochaya S,Osoegawa K,Pai G,Parsons M,Pentony M,Pettersson U,Pop M,Ramirez JL,Rinta J,Robertson L,Salzberg SL,Sanchez DO,Seyler A,Sharma R,Shetty J,Simpson AJ,Sisk E,Tammi MT,Tarleton R,Teixeira S,Van Aken S,Vogt C,Ward PN,Wickstead B,Wortman J,White O,Fraser CM,Stuart KD,Andersson B

Comparative genomics of trypanosomatid parasitic protozoa.
Science (New York, N.Y.)309(5733)404-9(2005 Jul)
El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N

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Tc00.1047053511467.20 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

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