Tc00.1047053509795.50 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

Gene
Clones
gRNAs

Summary

Gene Symbol	Tc00.1047053509795.50
Entrez Gene ID	3539471
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Trypanosoma cruzi strain CL Brener

mRNA and Protein(s)

mRNA	Protein	Name
XM_803848.1	XP_808941.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Worthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K, Englund PT, Fazelina G, Feldblyum T, Ferella M, Frasch AC, Gull K, Horn D, Hou L, Huang Y, Kindlund E, Klingbeil M, Kluge S, Koo H, Lacerda D, Levin MJ, Lorenzi H, Louie T, Machado CR, McCulloch R, McKenna A, Mizuno Y, Mottram JC, Nelson S, Ochaya S, Osoegawa K, Pai G, Parsons M, Pentony M, Pettersson U, Pop M, Ramirez JL, Rinta J, Robertson L, Salzberg SL, Sanchez DO, Seyler A, Sharma R, Shetty J, Simpson AJ, Sisk E, Tammi MT, Tarleton R, Teixeira S, Van Aken S, Vogt C, Ward PN, Wickstead B, Wortman J, White O, Fraser CM, Stuart KD, Andersson B
Science (New York, N.Y.)309(5733)409-15(2005 Jul)

Comparative genomics of trypanosomatid parasitic protozoa.
El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran AN, Wortman JR, Alsmark UC, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N
Science (New York, N.Y.)309(5733)404-9(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tc00.1047053509795.50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tc00.1047053509795.50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTb04364	XM_803848.1 Latest version!	Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tc00.1047053509795.50 ( XM_803848.1 ) cDNA ORF clone, Trypanosoma cruzi strain CL Brener -> XP_808941.1 Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.

Price & Availability↑

CloneID	OTb04364
Clone ID Related Accession (Same CDS sequence)	XM_803848.1
Accession Version	XM_803848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1225382400000
Organism	Trypanosoma cruzi strain CL Brener
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001849501). COMPLETENESS: incomplete on both ends.

ATGGCGTCAC TTTCATTAAG GCAAGTCGGT GTACTTAAAC ATGCGGAAGC GGTGTCGCAA 
TCCTTCCCGT TATGTGTCAC ATATGTTGAG CTGATGAAAG GCGCATACCT TTTCGCTTCG 
GTGAATGCCG CGAAAAACAG GTGTACTGTG GAGTTTATTT TGGACACCAA TAATAATAAT 
ACTAATGCTG CGGCTGCGGA TTCCACTTTT ACCACCAACA CCGTCGTGAT GGATGCCAAA 
TCTCATTCTT CAGCAGCAAG TGAGGTGGTG GAACATGAAA CGTTACTTGA AAATCTTCAA 
AGCAGCTTTC GAATACCAAA GTTCTTTCAG GTGCTGGAAG AAGAGGGAAT GATTACGGCG 
ATTGAGCTTG CTTGTGATTA CCGAAAGGTA GAAGACTCTA GAGAAGTCTT ACCTAAAAAG 
AAACGCGCGT TGGAATCTAC TTATTTGCAT TTGTTTATTG GAACATCCAA CGGGACAGTC 
TTTATTTGCA ATGCACTCCG TGGGACAATC ATGGCGCTGG CGCAGTTTCA GTACCATGGA 
GTCTTTACAC ACAGTCAGCT TGAAAAGTCC CAAGGAGATG AGACGCCACC GACGTGTCGG 
AATGAATCAG TCGTTCGTTT CGTTTTGCAT GGCACTGGGC CAGATGTACG TTTCAATGCA 
AACATCGCTC AAAGTACCGC TTCTCTGCCG ATAGGGACTC TCCACGCGGT GTGCGTCGTC 
CACAGTAGGG GAAGGGTCGT ATTACTAAGT CGAAATGTCC TTGACATTTT CCTTTCTGTG 
GCAGAGCAAC GTCTTGATGG AAAACGCCCA TACTTTCTCT TGGAGTGGTC GCCTGATAGC 
AGTATCAGCT CATTTCCTTC TGCTGGACCA GCTGCCCATT TTGCCTCTTG CATAGAATCC 
GTCTTTATCG TCTCAGAGGA AGAGCGGTAC TCTGCCGTGG AGTTGCGGAC ACAACCGATC 
ACGTCGAATA GTCCTATGGG ACGCAGTATT CGTGATGCAG CCTTCTTTTT TGGCAACCTT 
TCGGACACGG CGGCGATTCT CCGCAATGGC CCACAGGCCA TGGAATCTCT TATTTTGTGT 
GGTGAGGCTC CAGCACTTTC CATGTACACC TTTGAAAGTA CACGTGCCGC CTTTTCTGCA 
CGGAAAACTG TAAGAGCTGT GGTCTCGGTC CTGGGTGGAA TGGCGCGGAG AATATGGCAA 
AGGTCCTCAG AAAATGAAGA TAGGGGTGCA ATGAAACCGC AACGTTTAGC CAAGCGACAT 
CAACAAGCGA AGGATGCTTT TTTTGAGGCT GACATAGTGT TTAATGTTGT CCAGGTGGAC 
CCCACCTTTC AATGGGCTGC TTGTTACAGT GAGAGTTCTG GACGTATTTA TTTGTATGAC 
TTGATGGGCG GTGCCCTCTG GCGGGTGATG AAGGGTTGCC GTTCTGCTAA ATTTCAATGG 
TGCGTAGCTA CCATTGCTGG AAAACGTATG TTATTGCTTG TTGTGCACCT ACTACTTCGT 
TATGCTGTGG AGGTGTACTC TATACGTTTG GGAGAACGCC TGGCAGCTCG CCACGTTCCT 
CAGGGATCCA TTCTGCTTCG TCCAGAATCC GTCTCCTCTG CATTGTCTAT CCTCCTACTA 
ACGCCCTTCA GAGAAGTCAT ACGGGTAAAT GTGGAGCTGA ACGCGAAGAA GGAAGAAGAA 
ATTCCTTCTT CATTTTTATT TGCTGATCCT TCTGCTTTAC TGAATGAGGG AGAAAGTTCT 
TTTAAGACGT TGCGGGAAAA AGGGTACCAA ATAATGCCGC GAGATATTTT TATATCAGCA 
TTGCGTTTGC CGCTTCCTAC TCCAGTGACA GACGAGAGGC TTTTCTCCGC CTACCGATTG 
CAGATGAAGA AATTGGAGAA TACTGTAAAG AGTAAATTTT CGCCTGGGGT ATCTGATGAA 
CGTTGTTTCC CTATGGGTAT GGATACGACA GTGGAGACTG TTCCACGTAA CATTACCGCG 
GCCCAATGTC TGAATTATTT GGAACTGCGA CTTGCTTGTG TGACAAATTA TCATCGGGCA 
ATGATGTTGA GAGGGTTAAA TGATGGAATG CAGCAGCCGG GCATGAAGGC GTCAAAGTTG 
CCAAATAGCG AGGCTCTCTT TTCGGAGGAG GGGGAATCGC ATCCATCTCT TTTACAGGGA 
GTGAGTGCAG CATCGGAGAT GCTCTTCAGT GGCGGTTTTC CAGAGTTTGT GCATTTCTTG 
GAGGATATTC TTCCCTCTAT GCAACGGCTA TTGGCCATGC ATAAGGACGC TCCACCAGAA 
ACTAATTTTA CACAAATTCC TTCGATTCCT TTGGATGCCT TTTTAAAACA TTTTCATTGT 
GGGGGGTATA AGTTGGAATT TTTGCGTGAC AAGATGTGGG CTTTTGAGAA TGCGAGCGAT 
GCAGAGGAGG ATATATGGGC TGAGATTGGG GCCGTATTTT ACAGCTCTGC TGGCCGTGGC 
CTCGGCTCTT TATTGCAGCA GTTGGAGGTC TTTAGTGCAT TGGGTTTTCA AACTGTGGAT 
GTAGCGGTGA TTTCCCTGTC TTGGCTATGT TGTTCTTTTG CCAGGCGGCC TACGTTGGCC 
TCTTTAGGGT CATTATCAGA TCTTGTTTTT TTGTTGAAAA CGTGTAATGA CGCGGCATTT 
CTTACGGGTG TGGAACATGT TCCATGTTTT ACAACGGGAA TTGCTGGTAC GAACTATGGG 
GGAAATGAAG CATGCGTACT ATTGCTGATT CTGTGCATGA TCATACGTCA ACACGAAAAG 
CCTGTCGGGC ATGCTTATTA TACTCGTTAT TTGCGTTTAT TGCGTCAATT GTTGGCATTT 
CGAGTGATGC TTCATTGCTG GAAGAGTCAA CTTCAAGCAT CCATCACGGA TTCGGGAAAA 
AAGTGTTATC AAAGAATACG CATGTGCGGT TTGCTTCCAT GCGAGGGGGG TGATACATTG 
AGTGCGTATT GCTTCGGAAT TTTCCCCACC TCTGTGGCTC AGTTTCTGTG TGAGAATATC 
ATGGGGAGAG ACGAAATGAA GAGGGCCCTG GGTGGGGTAG ACATGGTTGA ATTATTCAAA 
TTAATTTCTA TTAGGAAAGA AACGTTTGAA TTGAATGTAG GGACCCCATG GTATAAGGAA 
CGAACATGGA GTCATGTTGT CTTTTCAGAG CGCGTGTTGA AACCAATTCT ATCTGTCACT 
GCAGCTCTCA TGCCCAAATC GCCTCAAGAA CGGCCATGGA TTCACACAGC AACGCCACAA 
GAGGTGGCAG GATTAGGTTT GATCCTTGTG GTAGAGTTGA TCCTCCTTGA GTGGGAATTG 
GAACCTCTTC GTGAGGAGCC TCTGGCATTT ATTCAGAAGA ATTTGGCCCC AGATGCCAAC 
CTCTTTGAGG GGAGTGACGA TCTTGTGAAG AGTCTGGCGC GGCGTCCAAA GGGAAGTCGT 
TCCACGCGTG ATTACTTTAA CAGTTGCCGC GAACTTTTAA CACTGGCTCA AGGCGTTTTG 
GCTGACATTG TGGGGGATGC GAGTAACTTA GAGAGTTGTG AGAAGGTCGT TGAGGCGTGG 
TCTTCATTGT TGTCCTATGA CAACAACGCA CTTTTCGCAC ATGTGCCTCT TCTCATGCGG 
CAACAGCTTG AACCTCTCAT AAAATTGTTT CGTACCAGTC CCGAGCCGTT AGTAGGACGT 
ATTCGTGAAG TCTCCTCTTT TCTGGACACC ATTATGTTTT TTGCCTTTAC GGAGGTCGGC 
ACCCAACTTT CTTCCGTCGC CTCACCATCC GCGTCTCTCT CAGTTCCGTG GGACTCCATG 
TTTGACGCGA GGGAGAGAAT GCTGCACCTG CGCCCGGATG GCGACTTTAC GGAGTTCCCG 
GAGAGGCAAC AGATGGAGAC TAAAATGCAA ATGCGTAGCT CATTCCTAAA AGAGTTTGCC 
TTTATGAATT ATAGGAGCAC GTATACAGAC GGATCTGGTG AACGTGTTGC ACAATTGGGA 
GAGGCATTGG GACTAAATCG TATTGTTGGC GATATATCCC AGCTTATTCT GTTTGATGCC 
GCGGCGCGTC ATTTATGTTC AAATACAGAG TTGCATCGTC TACGGAACGT TGTAGCAAGT 
CGCCATTTGG CTGCTCGCAT AGCTGCAGCA GACTTTCGGA TTATTGTCGC CAGTTGCTTG 
AAGTACTACA ACGAGTTGAA AAGAGGCATT CCAAAAGAAA ATCGATTGGA GATTCAAAGG 
GTCGCACTGA AAATCAAGAG CATGGATAGT GCAATGACGG AGGAGTGTCG CTCTTGGATA 
CAGATGGGAG ATGCAAAAAA GGGTCTTGAC ACGAGATTGG GAGAGGACGT GACGTCAAGA 
TCGCCTCTTG AACATGAAAT AGTCGCGTGC CCGAGTTGGT ACACCTTGAA TGATATTCGC 
GCCGTTGAGC GTAGGGTGAA GGAGCGACTA CAACAGCCGC GAAGCCCCGA AGCATTCTCT 
GATTTCAACG CAGTTCTCTT CACTTTGGCA TGTGTTGCTG CGGAGGGGAG AACTGAGTTG 
GAGGGAGTCG CACGTCGAGT CGCCGCAGAA CTCCCCGCGG TTTCAAGCCG CCTTGCCAAT 
TTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_808941.1
CDS	1..4569
Translation	MASLSLRQVGVLKHAEAVSQSFPLCVTYVELMKGAYLFASVNAAKNRCTVEFILDTNNNNTNAAAADSTFTTNTVVMDAKSHSSAASEVVEHETLLENLQSSFRIPKFFQVLEEEGMITAIELACDYRKVEDSREVLPKKKRALESTYLHLFIGTSNGTVFICNALRGTIMALAQFQYHGVFTHSQLEKSQGDETPPTCRNESVVRFVLHGTGPDVRFNANIAQSTASLPIGTLHAVCVVHSRGRVVLLSRNVLDIFLSVAEQRLDGKRPYFLLEWSPDSSISSFPSAGPAAHFASCIESVFIVSEEERYSAVELRTQPITSNSPMGRSIRDAAFFFGNLSDTAAILRNGPQAMESLILCGEAPALSMYTFESTRAAFSARKTVRAVVSVLGGMARRIWQRSSENEDRGAMKPQRLAKRHQQAKDAFFEADIVFNVVQVDPTFQWAACYSESSGRIYLYDLMGGALWRVMKGCRSAKFQWCVATIAGKRMLLLVVHLLLRYAVEVYSIRLGERLAARHVPQGSILLRPESVSSALSILLLTPFREVIRVNVELNAKKEEEIPSSFLFADPSALLNEGESSFKTLREKGYQIMPRDIFISALRLPLPTPVTDERLFSAYRLQMKKLENTVKSKFSPGVSDERCFPMGMDTTVETVPRNITAAQCLNYLELRLACVTNYHRAMMLRGLNDGMQQPGMKASKLPNSEALFSEEGESHPSLLQGVSAASEMLFSGGFPEFVHFLEDILPSMQRLLAMHKDAPPETNFTQIPSIPLDAFLKHFHCGGYKLEFLRDKMWAFENASDAEEDIWAEIGAVFYSSAGRGLGSLLQQLEVFSALGFQTVDVAVISLSWLCCSFARRPTLASLGSLSDLVFLLKTCNDAAFLTGVEHVPCFTTGIAGTNYGGNEACVLLLILCMIIRQHEKPVGHAYYTRYLRLLRQLLAFRVMLHCWKSQLQASITDSGKKCYQRIRMCGLLPCEGGDTLSAYCFGIFPTSVAQFLCENIMGRDEMKRALGGVDMVELFKLISIRKETFELNVGTPWYKERTWSHVVFSERVLKPILSVTAALMPKSPQERPWIHTATPQEVAGLGLILVVELILLEWELEPLREEPLAFIQKNLAPDANLFEGSDDLVKSLARRPKGSRSTRDYFNSCRELLTLAQGVLADIVGDASNLESCEKVVEAWSSLLSYDNNALFAHVPLLMRQQLEPLIKLFRTSPEPLVGRIREVSSFLDTIMFFAFTEVGTQLSSVASPSASLSVPWDSMFDARERMLHLRPDGDFTEFPERQQMETKMQMRSSFLKEFAFMNYRSTYTDGSGERVAQLGEALGLNRIVGDISQLILFDAAARHLCSNTELHRLRNVVASRHLAARIAAADFRIIVASCLKYYNELKRGIPKENRLEIQRVALKIKSMDSAMTEECRSWIQMGDAKKGLDTRLGEDVTSRSPLEHEIVACPSWYTLNDIRAVERRVKERLQQPRSPEAFSDFNAVLFTLACVAAEGRTELEGVARRVAAELPAVSSRLANLL

Target ORF information:

RefSeq Version	XM_803848.1
Organism	Trypanosoma cruzi strain CL Brener
Definition	Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_803848.1

ORF Insert Sequence:

ATGGCGTCAC TTTCATTAAG GCAAGTCGGT GTACTTAAAC ATGCGGAAGC GGTGTCGCAA 
TCCTTCCCGT TATGTGTCAC ATATGTTGAG CTGATGAAAG GCGCATACCT TTTCGCTTCG 
GTGAATGCCG CGAAAAACAG GTGTACTGTG GAGTTTATTT TGGACACCAA TAATAATAAT 
ACTAATGCTG CGGCTGCGGA TTCCACTTTT ACCACCAACA CCGTCGTGAT GGATGCCAAA 
TCTCATTCTT CAGCAGCAAG TGAGGTGGTG GAACATGAAA CGTTACTTGA AAATCTTCAA 
AGCAGCTTTC GAATACCAAA GTTCTTTCAG GTGCTGGAAG AAGAGGGAAT GATTACGGCG 
ATTGAGCTTG CTTGTGATTA CCGAAAGGTA GAAGACTCTA GAGAAGTCTT ACCTAAAAAG 
AAACGCGCGT TGGAATCTAC TTATTTGCAT TTGTTTATTG GAACATCCAA CGGGACAGTC 
TTTATTTGCA ATGCACTCCG TGGGACAATC ATGGCGCTGG CGCAGTTTCA GTACCATGGA 
GTCTTTACAC ACAGTCAGCT TGAAAAGTCC CAAGGAGATG AGACGCCACC GACGTGTCGG 
AATGAATCAG TCGTTCGTTT CGTTTTGCAT GGCACTGGGC CAGATGTACG TTTCAATGCA 
AACATCGCTC AAAGTACCGC TTCTCTGCCG ATAGGGACTC TCCACGCGGT GTGCGTCGTC 
CACAGTAGGG GAAGGGTCGT ATTACTAAGT CGAAATGTCC TTGACATTTT CCTTTCTGTG 
GCAGAGCAAC GTCTTGATGG AAAACGCCCA TACTTTCTCT TGGAGTGGTC GCCTGATAGC 
AGTATCAGCT CATTTCCTTC TGCTGGACCA GCTGCCCATT TTGCCTCTTG CATAGAATCC 
GTCTTTATCG TCTCAGAGGA AGAGCGGTAC TCTGCCGTGG AGTTGCGGAC ACAACCGATC 
ACGTCGAATA GTCCTATGGG ACGCAGTATT CGTGATGCAG CCTTCTTTTT TGGCAACCTT 
TCGGACACGG CGGCGATTCT CCGCAATGGC CCACAGGCCA TGGAATCTCT TATTTTGTGT 
GGTGAGGCTC CAGCACTTTC CATGTACACC TTTGAAAGTA CACGTGCCGC CTTTTCTGCA 
CGGAAAACTG TAAGAGCTGT GGTCTCGGTC CTGGGTGGAA TGGCGCGGAG AATATGGCAA 
AGGTCCTCAG AAAATGAAGA TAGGGGTGCA ATGAAACCGC AACGTTTAGC CAAGCGACAT 
CAACAAGCGA AGGATGCTTT TTTTGAGGCT GACATAGTGT TTAATGTTGT CCAGGTGGAC 
CCCACCTTTC AATGGGCTGC TTGTTACAGT GAGAGTTCTG GACGTATTTA TTTGTATGAC 
TTGATGGGCG GTGCCCTCTG GCGGGTGATG AAGGGTTGCC GTTCTGCTAA ATTTCAATGG 
TGCGTAGCTA CCATTGCTGG AAAACGTATG TTATTGCTTG TTGTGCACCT ACTACTTCGT 
TATGCTGTGG AGGTGTACTC TATACGTTTG GGAGAACGCC TGGCAGCTCG CCACGTTCCT 
CAGGGATCCA TTCTGCTTCG TCCAGAATCC GTCTCCTCTG CATTGTCTAT CCTCCTACTA 
ACGCCCTTCA GAGAAGTCAT ACGGGTAAAT GTGGAGCTGA ACGCGAAGAA GGAAGAAGAA 
ATTCCTTCTT CATTTTTATT TGCTGATCCT TCTGCTTTAC TGAATGAGGG AGAAAGTTCT 
TTTAAGACGT TGCGGGAAAA AGGGTACCAA ATAATGCCGC GAGATATTTT TATATCAGCA 
TTGCGTTTGC CGCTTCCTAC TCCAGTGACA GACGAGAGGC TTTTCTCCGC CTACCGATTG 
CAGATGAAGA AATTGGAGAA TACTGTAAAG AGTAAATTTT CGCCTGGGGT ATCTGATGAA 
CGTTGTTTCC CTATGGGTAT GGATACGACA GTGGAGACTG TTCCACGTAA CATTACCGCG 
GCCCAATGTC TGAATTATTT GGAACTGCGA CTTGCTTGTG TGACAAATTA TCATCGGGCA 
ATGATGTTGA GAGGGTTAAA TGATGGAATG CAGCAGCCGG GCATGAAGGC GTCAAAGTTG 
CCAAATAGCG AGGCTCTCTT TTCGGAGGAG GGGGAATCGC ATCCATCTCT TTTACAGGGA 
GTGAGTGCAG CATCGGAGAT GCTCTTCAGT GGCGGTTTTC CAGAGTTTGT GCATTTCTTG 
GAGGATATTC TTCCCTCTAT GCAACGGCTA TTGGCCATGC ATAAGGACGC TCCACCAGAA 
ACTAATTTTA CACAAATTCC TTCGATTCCT TTGGATGCCT TTTTAAAACA TTTTCATTGT 
GGGGGGTATA AGTTGGAATT TTTGCGTGAC AAGATGTGGG CTTTTGAGAA TGCGAGCGAT 
GCAGAGGAGG ATATATGGGC TGAGATTGGG GCCGTATTTT ACAGCTCTGC TGGCCGTGGC 
CTCGGCTCTT TATTGCAGCA GTTGGAGGTC TTTAGTGCAT TGGGTTTTCA AACTGTGGAT 
GTAGCGGTGA TTTCCCTGTC TTGGCTATGT TGTTCTTTTG CCAGGCGGCC TACGTTGGCC 
TCTTTAGGGT CATTATCAGA TCTTGTTTTT TTGTTGAAAA CGTGTAATGA CGCGGCATTT 
CTTACGGGTG TGGAACATGT TCCATGTTTT ACAACGGGAA TTGCTGGTAC GAACTATGGG 
GGAAATGAAG CATGCGTACT ATTGCTGATT CTGTGCATGA TCATACGTCA ACACGAAAAG 
CCTGTCGGGC ATGCTTATTA TACTCGTTAT TTGCGTTTAT TGCGTCAATT GTTGGCATTT 
CGAGTGATGC TTCATTGCTG GAAGAGTCAA CTTCAAGCAT CCATCACGGA TTCGGGAAAA 
AAGTGTTATC AAAGAATACG CATGTGCGGT TTGCTTCCAT GCGAGGGGGG TGATACATTG 
AGTGCGTATT GCTTCGGAAT TTTCCCCACC TCTGTGGCTC AGTTTCTGTG TGAGAATATC 
ATGGGGAGAG ACGAAATGAA GAGGGCCCTG GGTGGGGTAG ACATGGTTGA ATTATTCAAA 
TTAATTTCTA TTAGGAAAGA AACGTTTGAA TTGAATGTAG GGACCCCATG GTATAAGGAA 
CGAACATGGA GTCATGTTGT CTTTTCAGAG CGCGTGTTGA AACCAATTCT ATCTGTCACT 
GCAGCTCTCA TGCCCAAATC GCCTCAAGAA CGGCCATGGA TTCACACAGC AACGCCACAA 
GAGGTGGCAG GATTAGGTTT GATCCTTGTG GTAGAGTTGA TCCTCCTTGA GTGGGAATTG 
GAACCTCTTC GTGAGGAGCC TCTGGCATTT ATTCAGAAGA ATTTGGCCCC AGATGCCAAC 
CTCTTTGAGG GGAGTGACGA TCTTGTGAAG AGTCTGGCGC GGCGTCCAAA GGGAAGTCGT 
TCCACGCGTG ATTACTTTAA CAGTTGCCGC GAACTTTTAA CACTGGCTCA AGGCGTTTTG 
GCTGACATTG TGGGGGATGC GAGTAACTTA GAGAGTTGTG AGAAGGTCGT TGAGGCGTGG 
TCTTCATTGT TGTCCTATGA CAACAACGCA CTTTTCGCAC ATGTGCCTCT TCTCATGCGG 
CAACAGCTTG AACCTCTCAT AAAATTGTTT CGTACCAGTC CCGAGCCGTT AGTAGGACGT 
ATTCGTGAAG TCTCCTCTTT TCTGGACACC ATTATGTTTT TTGCCTTTAC GGAGGTCGGC 
ACCCAACTTT CTTCCGTCGC CTCACCATCC GCGTCTCTCT CAGTTCCGTG GGACTCCATG 
TTTGACGCGA GGGAGAGAAT GCTGCACCTG CGCCCGGATG GCGACTTTAC GGAGTTCCCG 
GAGAGGCAAC AGATGGAGAC TAAAATGCAA ATGCGTAGCT CATTCCTAAA AGAGTTTGCC 
TTTATGAATT ATAGGAGCAC GTATACAGAC GGATCTGGTG AACGTGTTGC ACAATTGGGA 
GAGGCATTGG GACTAAATCG TATTGTTGGC GATATATCCC AGCTTATTCT GTTTGATGCC 
GCGGCGCGTC ATTTATGTTC AAATACAGAG TTGCATCGTC TACGGAACGT TGTAGCAAGT 
CGCCATTTGG CTGCTCGCAT AGCTGCAGCA GACTTTCGGA TTATTGTCGC CAGTTGCTTG 
AAGTACTACA ACGAGTTGAA AAGAGGCATT CCAAAAGAAA ATCGATTGGA GATTCAAAGG 
GTCGCACTGA AAATCAAGAG CATGGATAGT GCAATGACGG AGGAGTGTCG CTCTTGGATA 
CAGATGGGAG ATGCAAAAAA GGGTCTTGAC ACGAGATTGG GAGAGGACGT GACGTCAAGA 
TCGCCTCTTG AACATGAAAT AGTCGCGTGC CCGAGTTGGT ACACCTTGAA TGATATTCGC 
GCCGTTGAGC GTAGGGTGAA GGAGCGACTA CAACAGCCGC GAAGCCCCGA AGCATTCTCT 
GATTTCAACG CAGTTCTCTT CACTTTGGCA TGTGTTGCTG CGGAGGGGAG AACTGAGTTG 
GAGGGAGTCG CACGTCGAGT CGCCGCAGAA CTCCCCGCGG TTTCAAGCCG CCTTGCCAAT 
TTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
Science (New York, N.Y.)309(5733)409-15(2005 Jul)
El-Sayed NM,Myler PJ,Bartholomeu DC,Nilsson D,Aggarwal G,Tran AN,Ghedin E,Worthey EA,Delcher AL,Blandin G,Westenberger SJ,Caler E,Cerqueira GC,Branche C,Haas B,Anupama A,Arner E,Aslund L,Attipoe P,Bontempi E,Bringaud F,Burton P,Cadag E,Campbell DA,Carrington M,Crabtree J,Darban H,da Silveira JF,de Jong P,Edwards K,Englund PT,Fazelina G,Feldblyum T,Ferella M,Frasch AC,Gull K,Horn D,Hou L,Huang Y,Kindlund E,Klingbeil M,Kluge S,Koo H,Lacerda D,Levin MJ,Lorenzi H,Louie T,Machado CR,McCulloch R,McKenna A,Mizuno Y,Mottram JC,Nelson S,Ochaya S,Osoegawa K,Pai G,Parsons M,Pentony M,Pettersson U,Pop M,Ramirez JL,Rinta J,Robertson L,Salzberg SL,Sanchez DO,Seyler A,Sharma R,Shetty J,Simpson AJ,Sisk E,Tammi MT,Tarleton R,Teixeira S,Van Aken S,Vogt C,Ward PN,Wickstead B,Wortman J,White O,Fraser CM,Stuart KD,Andersson B

Comparative genomics of trypanosomatid parasitic protozoa.
Science (New York, N.Y.)309(5733)404-9(2005 Jul)
El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N

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Tc00.1047053509795.50 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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