VPS8 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS8
Entrez Gene ID	100934981
Full Name	VPS8, CORVET complex subunit
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012550009.1	XP_012405463.1	vacuolar protein sorting-associated protein 8 homolog
XM_031968081.1	XP_031823941.1	vacuolar protein sorting-associated protein 8 homolog isoform X1
XM_031968082.1	XP_031823942.1	vacuolar protein sorting-associated protein 8 homolog isoform X2
XM_023503838.1	XP_023359606.1	vacuolar protein sorting-associated protein 8 homolog
XM_031968080.1	XP_031823940.1	vacuolar protein sorting-associated protein 8 homolog isoform X1

Pathways from BioSystems
Gene Ontology

The following VPS8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa132549	XM_012550009.1 Latest version!	Sarcophilus harrisii vacuolar protein sorting 8 homolog (S. cerevisiae) (VPS8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa234978	XM_031968081.1 Latest version!	Sarcophilus harrisii VPS8 subunit of CORVET complex (VPS8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa234978	XM_031968080.1 Latest version!	Sarcophilus harrisii VPS8 subunit of CORVET complex (VPS8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa234979	XM_031968082.1 Latest version!	Sarcophilus harrisii VPS8 subunit of CORVET complex (VPS8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS8 ( XM_012550009.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012405463.1 Sarcophilus harrisii vacuolar protein sorting 8 homolog (S. cerevisiae) (VPS8), mRNA.

Price & Availability↑

CloneID	OSa132549
Clone ID Related Accession (Same CDS sequence)	XM_012550009.1
Accession Version	XM_012550009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	vacuolar protein sorting-associated protein 8 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003839030.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGGCGAGTG AAGTCGATCC CGAGAGTGAG GAGGCAGCCC CCTCGCCCCG CGGAGGCCTC 
GGGGAAGGCC TGGCCCAGGC CCTGGAGGCT TCCCTGCCCA GGCTCACCTG CATAGACCTG 
GACAAGGACC TGGAGTTCAG AAATGAGCTG ATCGATGACA AGGAGTTTGA TATTCCTCAG 
GTGGACACCC CGCCCACCCT CGAAAGCATT CTCAATGAGA CCGATGATGA AGACGAATCC 
TTTGTTCTTG AGGATCCAAC GCTGTTAAAT ATCGACACCT TTGACACGCA CTCCTACGAC 
ACGTCGTCGG TGGCAAGCTC CGACAGCGGT GATCGAACCA ATCTGAAGAG GAAGAAGAAG 
CTTCTTGATT CTTTTTCTCT CCATGGCTCA GTCATGCGGC ATTCGCTTCT GAAGGGGATC 
TCTGCCCAGA TCGTCTCGGC AGCTGACAAA GTTGATGCCG GCTTGCCTAC TGCAATAGCT 
GTATCCAGTC TGATCGCAGT GGGGACTTCT CATGGATTGG CCTTAATCTT CGATCAGAAT 
CAAGCCCTGC GCCTCTGCTT GGGCAGCACC AGCGTCGGGG GACAGTACGG GGCCATCTCT 
GCCCTCAGCA TCAACCACGA CTGCTCCAGA CTTCTCTGCG GCTTTGCCAA AGGCCAGATG 
ACCATGTGGG ACTTGGCCAG CGGGAAGCTT TTGAGATCAA TAACAGATGC TCACCCCCCA 
GGAACAGCAA TTCTGCACAT TAAGTTCACA GATGACCCAA CTCTGGCCAT TTGCAACGAC 
AGCGGCGGTT CTGTGTTTGA GCTGACATTT AAGCGGGTGA TGGGAGTGAG GACGTGTGAA 
TCCCGGTGTC TGTTCAGCGG GTCCAAGGGG GAAGTTTGCT GCATCGAGCC TCTGCACTCC 
AAGGCCGAGC TGAAGGATCA CCCCATAACC CAGTTCTCCT TGCTGGCCAT GGCGTCCCTA 
ACTAAAATAT TGGTCATTGG ACTGAAGCCA TCTCTGAAAG TGTGGATGAC TTTCCCCTAT 
GGACGGATGG ATCCTTCCAG CGTCCCGCTG CTGGCCTGGC ATTTTGTGGC CGCCAATAAC 
TACGTGAACC CCACGCTGGC CTTTTGCCGA GGGGACGTCG TTCACTTCCT GTTGGTGAAG 
CGGGATGAGT CGGGCGCCAT ACACGTCACC AAGCAAAAGC ACCTGCACCT CTACTATGAC 
CTCATTAACT TCACTTGGAT CAATTCCCGG ACGATCGTGC TCCTGGACAG TGTGGAGAAG 
CTGCATGTGA TCGACCGGCT GACGCAGGAG GAGCTGGAGA CGGTAGAAAT CTCCGAGGTG 
CAGCTGGTCT ACAACAGTAG CCACTTCAAG TCTCTGGCCA CGGGTGGCAA CGTCAGCCAG 
GCCCTGGCTC TGGTTGGAGA GAAAGCCTGT TACCAGTCCA TCAGCAGTTA TGGTGGCCAG 
ATCTTTTATT TGGGGACAAA ATCTGTTTAT GTGATGACGT TGAGGAGCTG GAGAGAGAGA 
GTGGACCATC TCCTGAAACA AGAGTGTCTG CCGGAGGCCT TGGCTCTCGC GTGGTCCTTC 
CACGAAGGGA AGGCCAAAGC TGTCGTTGGC TTGTCTGGGG ACGCCAGCAA GCGGAAGGCC 
GTCGTTGCCG ATCGGATGGT TGAAATCCTC TTCCACTGTA CGGACCGAGC TTTGAAAAAA 
TGCCCGGACC AGGGCAAGAT CCAGGTGATG GAGCAGCATT TTCAGGACAC GGTGCCCGTG 
GTTGTTGATT ACTGCCTTCG GCTGCAGCGC AGGGATCTCC TGTTTAGCCA AATGTACGAC 
AAGTTCAGTG AGAATTCGGT GGCCAAAGGA GTCTTCCTGG AGTGCCTGGA GCCGTACATT 
CTGAGTGACC AGCTGGTGGG GATCACCGCC CAGGTGATGA AGGATCTGCT CATTCACTTC 
CAAGATAAAA AGCTGATGGA GAATGTGGAG GCACTGATCG TGCACATGGA CATCACCAGC 
CTGGACATCC AGCAGGTGGT TCTCATGTGC TGGGAGAACC ATCGCTACGA CGCCATGATC 
TACGTCTACA ACAGCGGCAT GAACGAGTTC ATCAGCCCCA TGGAGAAACT TTTCAAGGTC 
ATTGCTCCTC CTCTGAGCGC AGGGAAGTCC CTGACAGATG AGCAGGTTAT CATGGGCAAC 
AAGCTTCTTG TGTACATCAG CTGCTGCTTG GCGGGCCGGG CTTACCCCCT GGGGGATATC 
CCTGAAGACC TGGTGCCCTT GGTTAAAAAC CAGGTCTTCG AGTTTCTGAT CCGCCTGCAT 
TCTGCGGAAG CCTCCTCGGA GGAGGAGATC TACCCCTACA TCTGCACCCT GCTTCACTTC 
GACACCAGGG AGTTCCTGAA CGTCCTGGCT CTGACTTTTG AAGATTTTAA AAATGACAAG 
CAGGCTCTGG AATACCAGCA GCGGATCGTG GACATCTTGC TGAAGGTCAT GGTGGAAAAT 
TCCGACTTTA CTCCTTCCCA AGTCGGGTGC CTCTTCACCT TCCTGGCCCG GCAGCTCGCC 
AAGCCCGACA ACACGCTCTT CGTGAACAGG ACGCTTTTTG ACCAGGTGCT CGAGTTCCTC 
TGCAGCCCCG ATGATGACTC CCGACACTCG GAGAGGCAGC AGGTCCTCCT CGAGTTGCTC 
CAGGCCGGAG GGATTGTGCA GTTTGAGGAG AGCCGGCTCA TCCGGATGGC CGAGAGAGCC 
GAGTTCTACC AAATTTGTGA GTTTATGTAT GAACGAGAGC ACCAGTACGA CAAGATCATC 
GACTGCTACC TCCGGGACCC CCTGAGAGAG GAAGAGGTGT TCAACTACGT CCACAACATC 
CTGTCCATTC CCGGGCACAG CCCCGAGGAG AAGCAGTCCG TGGGGCAAAA GGCCATGCAG 
CACATTGAGG AGCTCGTGTC TCTGAACCCC TGCAAAGCAG CAGAGCTGGT GGCCACCCAT 
TTCTCCGGGC AAGTTGAGGC GGTCATCCAG AAACTTCAGA ACCCGTTTCT GCTCTTCCAG 
TTTCTGAGGA GCCTTCTTGA CCCAAGGGAA GGAGTTTCCG TTAACCAAGA GTCTCTGCAC 
GCGTCCCCTT GCATCACGGA GCAGTTCATT GAGCTGCTGT GTCAGTATAA CCCTCACCAA 
GTGATTGACA CTCTGCAAGT CCTAGAGTCC TACCGGCTGG AAGAGACCAT CCAGATTACC 
CAGAAGTACC AGCTTCTTGA AGTATGTGCC TACCTCTTAG AGAAGAAAGG AGATGTTCAT 
GGTGCTTTCT TAATAATGCT CGAGAGACTA CAAAGCAAGC TCCAGCAGTT TACAAAGGAA 
AGTGAAAACT CGAGAGAGAG CTCTTCCCTC AAGGCCGTGG AAGAGATCTT GGTGGAGACC 
ATCGCCCTCT GCCAGAGGAA TTCACAGAGT CTGAACCAGC CCCAGCGAGA GGCGCTCTGG 
TTTCCCCTCT TGGAGGCAAT GATGGCCCCT CAGAAGCTGT CCAGCTCCTG CGCTCCCCCT 
CGCCTGGCAG AACCCTTGAA GTCTCTAACC ATGCAGGTGC TGAACAGCAT GGCAGCCTTC 
ATCGCTCTGC CGTCCATCTT GCAGCGAATC CTGCAGGATC CAGTTTATGG AAGAGGAAAA 
CTTGGCGAGA TCCAAGGACT GATCCTGGGA ATGCTGGACA CCTTTAACTA CGAACAAACT 
CTCCTGGAGA CCACCACCAG CCTCCTCAAC CACGACCTGC ACTGGTCCCT CTGTAACCTG 
CGGGCATCGG TCACCAGGGG GCTGAACCCA AAGCAGGATT ACTGCTGCAT CTGCCTGCAG 
CAGTACCGGC GGCGCCAGGA GGTGGCCGAC GACATCGTCG TCTTCAGCTG CGGCCATCTC 
TACCACTCCT TGTGCCTGCT GAACAAAGAG TGTGCCGTGG GCACGGGGAA CCAGGCCCGC 
TGGACGTGCT ACAGATGCAA TTCAAGCAAC AAGGCAGGGA AGCTCAGCGA AAAGGCATCT 
GAAGTGAAGA AGGGGCAGCT TGCCCAGGCC CAGGTGAAAA TGTCTCCTTC CTGTCCATCG 
CCCCGAGGAG ATGCTGCTGC GAAGAAGGCC CCGTCAGAAC TGGGTCTGGA CCCCCAGCAG 
ATCCAGGCTT TCGACCAGCT CTGCCGCCTT TACCGGGGAA CCTCCAGGCT TAGAGAAGGC 
CACAGGAACC CCGGGCCCAC AGATGGCCCC TGGCTTCTGG TCCAGCTCCT CTGCCCCGAA 
GAAAACCCCT TCTCCATGTG CCTCAGGGCC CTTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012405463.1
CDS	57..4295
Translation	MASEVDPESEEAAPSPRGGLGEGLAQALEASLPRLTCIDLDKDLEFRNELIDDKEFDIPQVDTPPTLESILNETDDEDESFVLEDPTLLNIDTFDTHSYDTSSVASSDSGDRTNLKRKKKLLDSFSLHGSVMRHSLLKGISAQIVSAADKVDAGLPTAIAVSSLIAVGTSHGLALIFDQNQALRLCLGSTSVGGQYGAISALSINHDCSRLLCGFAKGQMTMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRVMGVRTCESRCLFSGSKGEVCCIEPLHSKAELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAANNYVNPTLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRLTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMTLRSWRERVDHLLKQECLPEALALAWSFHEGKAKAVVGLSGDASKRKAVVADRMVEILFHCTDRALKKCPDQGKIQVMEQHFQDTVPVVVDYCLRLQRRDLLFSQMYDKFSENSVAKGVFLECLEPYILSDQLVGITAQVMKDLLIHFQDKKLMENVEALIVHMDITSLDIQQVVLMCWENHRYDAMIYVYNSGMNEFISPMEKLFKVIAPPLSAGKSLTDEQVIMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSAEASSEEEIYPYICTLLHFDTREFLNVLALTFEDFKNDKQALEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAERAEFYQICEFMYEREHQYDKIIDCYLRDPLREEEVFNYVHNILSIPGHSPEEKQSVGQKAMQHIEELVSLNPCKAAELVATHFSGQVEAVIQKLQNPFLLFQFLRSLLDPREGVSVNQESLHASPCITEQFIELLCQYNPHQVIDTLQVLESYRLEETIQITQKYQLLEVCAYLLEKKGDVHGAFLIMLERLQSKLQQFTKESENSRESSSLKAVEEILVETIALCQRNSQSLNQPQREALWFPLLEAMMAPQKLSSSCAPPRLAEPLKSLTMQVLNSMAAFIALPSILQRILQDPVYGRGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNHDLHWSLCNLRASVTRGLNPKQDYCCICLQQYRRRQEVADDIVVFSCGHLYHSLCLLNKECAVGTGNQARWTCYRCNSSNKAGKLSEKASEVKKGQLAQAQVKMSPSCPSPRGDAAAKKAPSELGLDPQQIQAFDQLCRLYRGTSRLREGHRNPGPTDGPWLLVQLLCPEENPFSMCLRALV

Target ORF information:

RefSeq Version	XM_012550009.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii vacuolar protein sorting 8 homolog (S. cerevisiae) (VPS8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012550009.1

ORF Insert Sequence:

ATGGCGAGTG AAGTCGATCC CGAGAGTGAG GAGGCAGCCC CCTCGCCCCG CGGAGGCCTC 
GGGGAAGGCC TGGCCCAGGC CCTGGAGGCT TCCCTGCCCA GGCTCACCTG CATAGACCTG 
GACAAGGACC TGGAGTTCAG AAATGAGCTG ATCGATGACA AGGAGTTTGA TATTCCTCAG 
GTGGACACCC CGCCCACCCT CGAAAGCATT CTCAATGAGA CCGATGATGA AGACGAATCC 
TTTGTTCTTG AGGATCCAAC GCTGTTAAAT ATCGACACCT TTGACACGCA CTCCTACGAC 
ACGTCGTCGG TGGCAAGCTC CGACAGCGGT GATCGAACCA ATCTGAAGAG GAAGAAGAAG 
CTTCTTGATT CTTTTTCTCT CCATGGCTCA GTCATGCGGC ATTCGCTTCT GAAGGGGATC 
TCTGCCCAGA TCGTCTCGGC AGCTGACAAA GTTGATGCCG GCTTGCCTAC TGCAATAGCT 
GTATCCAGTC TGATCGCAGT GGGGACTTCT CATGGATTGG CCTTAATCTT CGATCAGAAT 
CAAGCCCTGC GCCTCTGCTT GGGCAGCACC AGCGTCGGGG GACAGTACGG GGCCATCTCT 
GCCCTCAGCA TCAACCACGA CTGCTCCAGA CTTCTCTGCG GCTTTGCCAA AGGCCAGATG 
ACCATGTGGG ACTTGGCCAG CGGGAAGCTT TTGAGATCAA TAACAGATGC TCACCCCCCA 
GGAACAGCAA TTCTGCACAT TAAGTTCACA GATGACCCAA CTCTGGCCAT TTGCAACGAC 
AGCGGCGGTT CTGTGTTTGA GCTGACATTT AAGCGGGTGA TGGGAGTGAG GACGTGTGAA 
TCCCGGTGTC TGTTCAGCGG GTCCAAGGGG GAAGTTTGCT GCATCGAGCC TCTGCACTCC 
AAGGCCGAGC TGAAGGATCA CCCCATAACC CAGTTCTCCT TGCTGGCCAT GGCGTCCCTA 
ACTAAAATAT TGGTCATTGG ACTGAAGCCA TCTCTGAAAG TGTGGATGAC TTTCCCCTAT 
GGACGGATGG ATCCTTCCAG CGTCCCGCTG CTGGCCTGGC ATTTTGTGGC CGCCAATAAC 
TACGTGAACC CCACGCTGGC CTTTTGCCGA GGGGACGTCG TTCACTTCCT GTTGGTGAAG 
CGGGATGAGT CGGGCGCCAT ACACGTCACC AAGCAAAAGC ACCTGCACCT CTACTATGAC 
CTCATTAACT TCACTTGGAT CAATTCCCGG ACGATCGTGC TCCTGGACAG TGTGGAGAAG 
CTGCATGTGA TCGACCGGCT GACGCAGGAG GAGCTGGAGA CGGTAGAAAT CTCCGAGGTG 
CAGCTGGTCT ACAACAGTAG CCACTTCAAG TCTCTGGCCA CGGGTGGCAA CGTCAGCCAG 
GCCCTGGCTC TGGTTGGAGA GAAAGCCTGT TACCAGTCCA TCAGCAGTTA TGGTGGCCAG 
ATCTTTTATT TGGGGACAAA ATCTGTTTAT GTGATGACGT TGAGGAGCTG GAGAGAGAGA 
GTGGACCATC TCCTGAAACA AGAGTGTCTG CCGGAGGCCT TGGCTCTCGC GTGGTCCTTC 
CACGAAGGGA AGGCCAAAGC TGTCGTTGGC TTGTCTGGGG ACGCCAGCAA GCGGAAGGCC 
GTCGTTGCCG ATCGGATGGT TGAAATCCTC TTCCACTGTA CGGACCGAGC TTTGAAAAAA 
TGCCCGGACC AGGGCAAGAT CCAGGTGATG GAGCAGCATT TTCAGGACAC GGTGCCCGTG 
GTTGTTGATT ACTGCCTTCG GCTGCAGCGC AGGGATCTCC TGTTTAGCCA AATGTACGAC 
AAGTTCAGTG AGAATTCGGT GGCCAAAGGA GTCTTCCTGG AGTGCCTGGA GCCGTACATT 
CTGAGTGACC AGCTGGTGGG GATCACCGCC CAGGTGATGA AGGATCTGCT CATTCACTTC 
CAAGATAAAA AGCTGATGGA GAATGTGGAG GCACTGATCG TGCACATGGA CATCACCAGC 
CTGGACATCC AGCAGGTGGT TCTCATGTGC TGGGAGAACC ATCGCTACGA CGCCATGATC 
TACGTCTACA ACAGCGGCAT GAACGAGTTC ATCAGCCCCA TGGAGAAACT TTTCAAGGTC 
ATTGCTCCTC CTCTGAGCGC AGGGAAGTCC CTGACAGATG AGCAGGTTAT CATGGGCAAC 
AAGCTTCTTG TGTACATCAG CTGCTGCTTG GCGGGCCGGG CTTACCCCCT GGGGGATATC 
CCTGAAGACC TGGTGCCCTT GGTTAAAAAC CAGGTCTTCG AGTTTCTGAT CCGCCTGCAT 
TCTGCGGAAG CCTCCTCGGA GGAGGAGATC TACCCCTACA TCTGCACCCT GCTTCACTTC 
GACACCAGGG AGTTCCTGAA CGTCCTGGCT CTGACTTTTG AAGATTTTAA AAATGACAAG 
CAGGCTCTGG AATACCAGCA GCGGATCGTG GACATCTTGC TGAAGGTCAT GGTGGAAAAT 
TCCGACTTTA CTCCTTCCCA AGTCGGGTGC CTCTTCACCT TCCTGGCCCG GCAGCTCGCC 
AAGCCCGACA ACACGCTCTT CGTGAACAGG ACGCTTTTTG ACCAGGTGCT CGAGTTCCTC 
TGCAGCCCCG ATGATGACTC CCGACACTCG GAGAGGCAGC AGGTCCTCCT CGAGTTGCTC 
CAGGCCGGAG GGATTGTGCA GTTTGAGGAG AGCCGGCTCA TCCGGATGGC CGAGAGAGCC 
GAGTTCTACC AAATTTGTGA GTTTATGTAT GAACGAGAGC ACCAGTACGA CAAGATCATC 
GACTGCTACC TCCGGGACCC CCTGAGAGAG GAAGAGGTGT TCAACTACGT CCACAACATC 
CTGTCCATTC CCGGGCACAG CCCCGAGGAG AAGCAGTCCG TGGGGCAAAA GGCCATGCAG 
CACATTGAGG AGCTCGTGTC TCTGAACCCC TGCAAAGCAG CAGAGCTGGT GGCCACCCAT 
TTCTCCGGGC AAGTTGAGGC GGTCATCCAG AAACTTCAGA ACCCGTTTCT GCTCTTCCAG 
TTTCTGAGGA GCCTTCTTGA CCCAAGGGAA GGAGTTTCCG TTAACCAAGA GTCTCTGCAC 
GCGTCCCCTT GCATCACGGA GCAGTTCATT GAGCTGCTGT GTCAGTATAA CCCTCACCAA 
GTGATTGACA CTCTGCAAGT CCTAGAGTCC TACCGGCTGG AAGAGACCAT CCAGATTACC 
CAGAAGTACC AGCTTCTTGA AGTATGTGCC TACCTCTTAG AGAAGAAAGG AGATGTTCAT 
GGTGCTTTCT TAATAATGCT CGAGAGACTA CAAAGCAAGC TCCAGCAGTT TACAAAGGAA 
AGTGAAAACT CGAGAGAGAG CTCTTCCCTC AAGGCCGTGG AAGAGATCTT GGTGGAGACC 
ATCGCCCTCT GCCAGAGGAA TTCACAGAGT CTGAACCAGC CCCAGCGAGA GGCGCTCTGG 
TTTCCCCTCT TGGAGGCAAT GATGGCCCCT CAGAAGCTGT CCAGCTCCTG CGCTCCCCCT 
CGCCTGGCAG AACCCTTGAA GTCTCTAACC ATGCAGGTGC TGAACAGCAT GGCAGCCTTC 
ATCGCTCTGC CGTCCATCTT GCAGCGAATC CTGCAGGATC CAGTTTATGG AAGAGGAAAA 
CTTGGCGAGA TCCAAGGACT GATCCTGGGA ATGCTGGACA CCTTTAACTA CGAACAAACT 
CTCCTGGAGA CCACCACCAG CCTCCTCAAC CACGACCTGC ACTGGTCCCT CTGTAACCTG 
CGGGCATCGG TCACCAGGGG GCTGAACCCA AAGCAGGATT ACTGCTGCAT CTGCCTGCAG 
CAGTACCGGC GGCGCCAGGA GGTGGCCGAC GACATCGTCG TCTTCAGCTG CGGCCATCTC 
TACCACTCCT TGTGCCTGCT GAACAAAGAG TGTGCCGTGG GCACGGGGAA CCAGGCCCGC 
TGGACGTGCT ACAGATGCAA TTCAAGCAAC AAGGCAGGGA AGCTCAGCGA AAAGGCATCT 
GAAGTGAAGA AGGGGCAGCT TGCCCAGGCC CAGGTGAAAA TGTCTCCTTC CTGTCCATCG 
CCCCGAGGAG ATGCTGCTGC GAAGAAGGCC CCGTCAGAAC TGGGTCTGGA CCCCCAGCAG 
ATCCAGGCTT TCGACCAGCT CTGCCGCCTT TACCGGGGAA CCTCCAGGCT TAGAGAAGGC 
CACAGGAACC CCGGGCCCAC AGATGGCCCC TGGCTTCTGG TCCAGCTCCT CTGCCCCGAA 
GAAAACCCCT TCTCCATGTG CCTCAGGGCC CTTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS8 ( XM_031968081.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823941.1 Sarcophilus harrisii VPS8 subunit of CORVET complex (VPS8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa234978
Clone ID Related Accession (Same CDS sequence)	XM_031968081.1 , XM_031968080.1
Accession Version	XM_031968081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGTG AAGTCGATCC CGAGAGTGAG GAGGCAGCCC CCTCGCCCCG CGGAGGCCTC 
GGGGAAGGCC TGGCCCAGGC CCTGGAGGCT TCCCTGCCCA GGCTCACCTG CATAGACCTG 
GACAAGGACC TGGAGTTCAG AAATGAGCTG ATCGATGACA AGGAGTTTGA TATTCCTCAG 
GTGGACACCC CGCCCACCCT CGAAAGCATT CTCAATGAGA CCGATGATGA AGACGAATCC 
TTTGTTCTTG AGGATCCAAC GCTGTTAAAT ATCGACACCT TTGACACGCA CTCCTACGAC 
ACGTCGTCGG TGGCAAGCTC CGACAGCGGT GATCGAACCA ATCTGAAGAG GAAGAAGAAG 
CTTCTTGATT CTTTTTCTCT CCATGGCTCA GTCATGCGGC ATTCGCTTCT GAAGGGGATC 
TCTGCCCAGA TCGTCTCGGC AGCTGACAAA GTTGATGCCG GCTTGCCTAC TGCAATAGCT 
GTATCCAGTC TGATCGCAGT GGGGACTTCT CATGGATTGG CCTTAATCTT CGGAAAAGAT 
CAGAATCAAG CCCTGCGCCT CTGCTTGGGC AGCACCAGCG TCGGGGGACA GTACGGGGCC 
ATCTCTGCCC TCAGCATCAA CCACGACTGC TCCAGACTTC TCTGCGGCTT TGCCAAAGGC 
CAGATGACCA TGTGGGACTT GGCCAGCGGG AAGCTTTTGA GATCAATAAC AGATGCTCAC 
CCCCCAGGAA CAGCAATTCT GCACATTAAG TTCACAGATG ACCCAACTCT GGCCATTTGC 
AACGACAGCG GCGGTTCTGT GTTTGAGCTG ACATTTAAGC GGGTGATGGG AGTGAGGACG 
TGTGAATCCC GGTGTCTGTT CAGCGGGTCC AAGGGGGAAG TTTGCTGCAT CGAGCCTCTG 
CACTCCAAGG CCGAGCTGAA GGATCACCCC ATAACCCAGT TCTCCTTGCT GGCCATGGCG 
TCCCTAACTA AAATATTGGT CATTGGACTG AAGCCATCTC TGAAAGTGTG GATGACTTTC 
CCCTATGGAC GGATGGATCC TTCCAGCGTC CCGCTGCTGG CCTGGCATTT TGTGGCCGCC 
AATAACTACG TGAACCCCAC GCTGGCCTTT TGCCGAGGGG ACGTCGTTCA CTTCCTGTTG 
GTGAAGCGGG ATGAGTCGGG CGCCATACAC GTCACCAAGC AAAAGCACCT GCACCTCTAC 
TATGACCTCA TTAACTTCAC TTGGATCAAT TCCCGGACGA TCGTGCTCCT GGACAGTGTG 
GAGAAGCTGC ATGTGATCGA CCGGCTGACG CAGGAGGAGC TGGAGACGGT AGAAATCTCC 
GAGGTGCAGC TGGTCTACAA CAGTAGCCAC TTCAAGTCTC TGGCCACGGG TGGCAACGTC 
AGCCAGGCCC TGGCTCTGGT TGGAGAGAAA GCCTGTTACC AGTCCATCAG CAGTTATGGT 
GGCCAGATCT TTTATTTGGG GACAAAATCT GTTTATGTGA TGACGTTGAG GAGCTGGAGA 
GAGAGAGTGG ACCATCTCCT GAAACAAGAG TGTCTGCCGG AGGCCTTGGC TCTCGCGTGG 
TCCTTCCACG AAGGGAAGGC CAAAGCTGTC GTTGGCTTGT CTGGGGACGC CAGCAAGCGG 
AAGGCCGTCG TTGCCGATCG GATGGTTGAA ATCCTCTTCC ACTGTACGGA CCGAGCTTTG 
AAAAAATGCC CGGACCAGGG CAAGATCCAG GTGATGGAGC AGCATTTTCA GGACACGGTG 
CCCGTGGTTG TTGATTACTG CCTTCGGCTG CAGCGCAGGG ATCTCCTGTT TAGCCAAATG 
TACGACAAGT TCAGTGAGAA TTCGGTGGCC AAAGGAGTCT TCCTGGAGTG CCTGGAGCCG 
TACATTCTGA GTGACCAGCT GGTGGGGATC ACCGCCCAGG TGATGAAGGA TCTGCTCATT 
CACTTCCAAG ATAAAAAGCT GATGGAGAAT GTGGAGGCAC TGATCGTGCA CATGGACATC 
ACCAGCCTGG ACATCCAGCA GGTGGTTCTC ATGTGCTGGG AGAACCATCT CTACGACGCC 
ATGATCTACG TCTACAACAG CGGCATGAAC GAGTTCATCA GCCCCATGGA GAAACTTTTC 
AAGGTCATTG CTCCTCCTCT GAGCGCAGGG AAGTCCCTGA CAGATGAGCA GGTTATCATG 
GGCAACAAGC TTCTTGTGTA CATCAGCTGC TGCTTGGCGG GCCGGGCTTA CCCCCTGGGG 
GATATCCCTG AAGACCTGGT GCCCTTGGTT AAAAACCAGG TCTTCGAGTT TCTGATCCGC 
CTGCATTCTG CGGAAGCCTC CTCGGAGGAG GAGATCTACC CCTACATCTG CACCCTGCTT 
CACTTCGACA CCAGGGAGTT CCTGAACGTC CTGGCTCTGA CTTTTGAAGA TTTTAAAAAT 
GACAAGCAGG CTCTGGAATA CCAGCAGCGG ATCGTGGACA TCTTGCTGAA GGTCATGGTG 
GAAAATTCCG ACTTTACTCC TTCCCAAGTC GGGTGCCTCT TCACCTTCCT GGCCCGGCAG 
CTCGCCAAGC CCGACAACAC GCTCTTCGTG AACAGGACGC TTTTTGACCA GGTGCTCGAG 
TTCCTCTGCA GCCCCGATGA TGACTCCCGA CACTCGGAGA GGCAGCAGGT CCTCCTCGAG 
TTGCTCCAGG CCGGAGGGAT TGTGCAGTTT GAGGAGAGCC GGCTCATCCG GATGGCCGAG 
AGAGCCGAGT TCTACCAAAT TTGTGAGTTT ATGTATGAAC GAGAGCACCA GTACGACAAG 
ATCATCGACT GCTACCTCCG GGACCCCCTG AGAGAGGAAG AGGTGTTCAA CTACGTCCAC 
AACATCCTGT CCATTCCCGG GCACAGCCCC GAGGAGAAGC AGTCCGTGGG GCAAAAGGCC 
ATGCAGCACA TTGAGGAGCT CGTGTCTCTG AACCCCTGCA AAGCAGCAGA GCTGGTGGCC 
ACCCATTTCT CCGGGCAAGT TGAGGCGGTC ATCCAGAAAC TTCAGAACCC GTTTCTGCTC 
TTCCAGTTTC TGAGGAGCCT TCTTGACCCA AGGGAAGGAG TTTCCGTTAA CCAAGAGTCT 
CTGCACGCGT CCCCTTGCAT CACGGAGCAG TTCATTGAGC TGCTGTGTCA GTATAACCCT 
CACCAAGTGA TTGACACTCT GCAAGTCCTA GAGTCCTACC GGCTGGAAGA GACCATCCAG 
ATTACCCAGA AGTACCAGCT TCTTGAAGTA TGTGCCTACC TCTTAGAGAA GAAAGGAGAT 
GTTCATGGTG CTTTCTTAAT AATGCTCGAG AGACTACAAA GCAAGCTCCA GCAGTTTACA 
AAGGAAAGTG AAAACTCGAG AGAGAGCTCT TCCCTCAAGG CCGTGGAAGA GATCTTGGTG 
GAGACCATCG CCCTCTGCCA GAGGAATTCA CAGAGTCTGA ACCAGCCCCA GCGAGAGGCG 
CTCTGGTTTC CCCTCTTGGA GGCAATGATG GCCCCTCAGA AGCTGTCCAG CTCCTGCGCT 
CCCCCTCGCC TGGCAGAACC CTTGAAGTCT CTAACCATGC AGGTGCTGAA CAGCATGGCA 
GCCTTCATCG CTCTGCCGTC CATCTTGCAG CGAATCCTGC AGGATCCAGT TTATGGAAGA 
GGAAAACTTG GCGAGATCCA AGGACTGATC CTGGGAATGC TGGACACCTT TAACTACGAA 
CAAACTCTCC TGGAGACCAC CACCAGCCTC CTCAACCACG ACCTGCACTG GTCCCTCTGT 
AACCTGCGGG CATCGGTCAC CAGGGGGCTG AACCCAAAGC AGGATTACTG CTGCATCTGC 
CTGCAGCAGT ACCGGCGGCG CCAGGAGGTG GCCGACGACA TCGTCGTCTT CAGCTGCGGC 
CATCTCTACC ACTCCTTGTG CCTGCTGAAC AAAGAGTGTG CCGTGGGCAC GGGGAACCAG 
GCCCGCTGGA CGTGCTACAG ATGCAATTCA AGCAACAAGG CAGGGAAGCT CAGCGAAAAG 
GCATCTGAAG TGAAGAAGGG GCAGCTTGCC CAGGCCCAGG TGAAAATGTC TCCTTCCTGT 
CCATCGCCCC GAGGAGATGC TGCTGCGAAG AAGGCCCCGT CAGAACTGGG TCTGGACCCC 
CAGCAGATCC AGGCTTTCGA CCAGCTCTGC CGCCTTTACC GGGGAACCTC CAGGCTGGCG 
CTGCTGACCG AGCTGTCCCA GAACCAGAGC GGGGAGAGTC GCCGGCTGCT GGGGGGCTCC 
AAGAGCAGCC CCGCCCTCAA CAGCATCTTC CAGAACGAGA ACTTCCAGCT GCACCTGAGC 
CCTCCCCCCC TGAGCGAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823941.1
CDS	116..4399
Translation	MASEVDPESEEAAPSPRGGLGEGLAQALEASLPRLTCIDLDKDLEFRNELIDDKEFDIPQVDTPPTLESILNETDDEDESFVLEDPTLLNIDTFDTHSYDTSSVASSDSGDRTNLKRKKKLLDSFSLHGSVMRHSLLKGISAQIVSAADKVDAGLPTAIAVSSLIAVGTSHGLALIFGKDQNQALRLCLGSTSVGGQYGAISALSINHDCSRLLCGFAKGQMTMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRVMGVRTCESRCLFSGSKGEVCCIEPLHSKAELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAANNYVNPTLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRLTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMTLRSWRERVDHLLKQECLPEALALAWSFHEGKAKAVVGLSGDASKRKAVVADRMVEILFHCTDRALKKCPDQGKIQVMEQHFQDTVPVVVDYCLRLQRRDLLFSQMYDKFSENSVAKGVFLECLEPYILSDQLVGITAQVMKDLLIHFQDKKLMENVEALIVHMDITSLDIQQVVLMCWENHLYDAMIYVYNSGMNEFISPMEKLFKVIAPPLSAGKSLTDEQVIMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSAEASSEEEIYPYICTLLHFDTREFLNVLALTFEDFKNDKQALEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAERAEFYQICEFMYEREHQYDKIIDCYLRDPLREEEVFNYVHNILSIPGHSPEEKQSVGQKAMQHIEELVSLNPCKAAELVATHFSGQVEAVIQKLQNPFLLFQFLRSLLDPREGVSVNQESLHASPCITEQFIELLCQYNPHQVIDTLQVLESYRLEETIQITQKYQLLEVCAYLLEKKGDVHGAFLIMLERLQSKLQQFTKESENSRESSSLKAVEEILVETIALCQRNSQSLNQPQREALWFPLLEAMMAPQKLSSSCAPPRLAEPLKSLTMQVLNSMAAFIALPSILQRILQDPVYGRGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNHDLHWSLCNLRASVTRGLNPKQDYCCICLQQYRRRQEVADDIVVFSCGHLYHSLCLLNKECAVGTGNQARWTCYRCNSSNKAGKLSEKASEVKKGQLAQAQVKMSPSCPSPRGDAAAKKAPSELGLDPQQIQAFDQLCRLYRGTSRLALLTELSQNQSGESRRLLGGSKSSPALNSIFQNENFQLHLSPPPLSED

Target ORF information:

RefSeq Version	XM_031968081.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii VPS8 subunit of CORVET complex (VPS8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031968081.1

ORF Insert Sequence:

ATGGCGAGTG AAGTCGATCC CGAGAGTGAG GAGGCAGCCC CCTCGCCCCG CGGAGGCCTC 
GGGGAAGGCC TGGCCCAGGC CCTGGAGGCT TCCCTGCCCA GGCTCACCTG CATAGACCTG 
GACAAGGACC TGGAGTTCAG AAATGAGCTG ATCGATGACA AGGAGTTTGA TATTCCTCAG 
GTGGACACCC CGCCCACCCT CGAAAGCATT CTCAATGAGA CCGATGATGA AGACGAATCC 
TTTGTTCTTG AGGATCCAAC GCTGTTAAAT ATCGACACCT TTGACACGCA CTCCTACGAC 
ACGTCGTCGG TGGCAAGCTC CGACAGCGGT GATCGAACCA ATCTGAAGAG GAAGAAGAAG 
CTTCTTGATT CTTTTTCTCT CCATGGCTCA GTCATGCGGC ATTCGCTTCT GAAGGGGATC 
TCTGCCCAGA TCGTCTCGGC AGCTGACAAA GTTGATGCCG GCTTGCCTAC TGCAATAGCT 
GTATCCAGTC TGATCGCAGT GGGGACTTCT CATGGATTGG CCTTAATCTT CGGAAAAGAT 
CAGAATCAAG CCCTGCGCCT CTGCTTGGGC AGCACCAGCG TCGGGGGACA GTACGGGGCC 
ATCTCTGCCC TCAGCATCAA CCACGACTGC TCCAGACTTC TCTGCGGCTT TGCCAAAGGC 
CAGATGACCA TGTGGGACTT GGCCAGCGGG AAGCTTTTGA GATCAATAAC AGATGCTCAC 
CCCCCAGGAA CAGCAATTCT GCACATTAAG TTCACAGATG ACCCAACTCT GGCCATTTGC 
AACGACAGCG GCGGTTCTGT GTTTGAGCTG ACATTTAAGC GGGTGATGGG AGTGAGGACG 
TGTGAATCCC GGTGTCTGTT CAGCGGGTCC AAGGGGGAAG TTTGCTGCAT CGAGCCTCTG 
CACTCCAAGG CCGAGCTGAA GGATCACCCC ATAACCCAGT TCTCCTTGCT GGCCATGGCG 
TCCCTAACTA AAATATTGGT CATTGGACTG AAGCCATCTC TGAAAGTGTG GATGACTTTC 
CCCTATGGAC GGATGGATCC TTCCAGCGTC CCGCTGCTGG CCTGGCATTT TGTGGCCGCC 
AATAACTACG TGAACCCCAC GCTGGCCTTT TGCCGAGGGG ACGTCGTTCA CTTCCTGTTG 
GTGAAGCGGG ATGAGTCGGG CGCCATACAC GTCACCAAGC AAAAGCACCT GCACCTCTAC 
TATGACCTCA TTAACTTCAC TTGGATCAAT TCCCGGACGA TCGTGCTCCT GGACAGTGTG 
GAGAAGCTGC ATGTGATCGA CCGGCTGACG CAGGAGGAGC TGGAGACGGT AGAAATCTCC 
GAGGTGCAGC TGGTCTACAA CAGTAGCCAC TTCAAGTCTC TGGCCACGGG TGGCAACGTC 
AGCCAGGCCC TGGCTCTGGT TGGAGAGAAA GCCTGTTACC AGTCCATCAG CAGTTATGGT 
GGCCAGATCT TTTATTTGGG GACAAAATCT GTTTATGTGA TGACGTTGAG GAGCTGGAGA 
GAGAGAGTGG ACCATCTCCT GAAACAAGAG TGTCTGCCGG AGGCCTTGGC TCTCGCGTGG 
TCCTTCCACG AAGGGAAGGC CAAAGCTGTC GTTGGCTTGT CTGGGGACGC CAGCAAGCGG 
AAGGCCGTCG TTGCCGATCG GATGGTTGAA ATCCTCTTCC ACTGTACGGA CCGAGCTTTG 
AAAAAATGCC CGGACCAGGG CAAGATCCAG GTGATGGAGC AGCATTTTCA GGACACGGTG 
CCCGTGGTTG TTGATTACTG CCTTCGGCTG CAGCGCAGGG ATCTCCTGTT TAGCCAAATG 
TACGACAAGT TCAGTGAGAA TTCGGTGGCC AAAGGAGTCT TCCTGGAGTG CCTGGAGCCG 
TACATTCTGA GTGACCAGCT GGTGGGGATC ACCGCCCAGG TGATGAAGGA TCTGCTCATT 
CACTTCCAAG ATAAAAAGCT GATGGAGAAT GTGGAGGCAC TGATCGTGCA CATGGACATC 
ACCAGCCTGG ACATCCAGCA GGTGGTTCTC ATGTGCTGGG AGAACCATCT CTACGACGCC 
ATGATCTACG TCTACAACAG CGGCATGAAC GAGTTCATCA GCCCCATGGA GAAACTTTTC 
AAGGTCATTG CTCCTCCTCT GAGCGCAGGG AAGTCCCTGA CAGATGAGCA GGTTATCATG 
GGCAACAAGC TTCTTGTGTA CATCAGCTGC TGCTTGGCGG GCCGGGCTTA CCCCCTGGGG 
GATATCCCTG AAGACCTGGT GCCCTTGGTT AAAAACCAGG TCTTCGAGTT TCTGATCCGC 
CTGCATTCTG CGGAAGCCTC CTCGGAGGAG GAGATCTACC CCTACATCTG CACCCTGCTT 
CACTTCGACA CCAGGGAGTT CCTGAACGTC CTGGCTCTGA CTTTTGAAGA TTTTAAAAAT 
GACAAGCAGG CTCTGGAATA CCAGCAGCGG ATCGTGGACA TCTTGCTGAA GGTCATGGTG 
GAAAATTCCG ACTTTACTCC TTCCCAAGTC GGGTGCCTCT TCACCTTCCT GGCCCGGCAG 
CTCGCCAAGC CCGACAACAC GCTCTTCGTG AACAGGACGC TTTTTGACCA GGTGCTCGAG 
TTCCTCTGCA GCCCCGATGA TGACTCCCGA CACTCGGAGA GGCAGCAGGT CCTCCTCGAG 
TTGCTCCAGG CCGGAGGGAT TGTGCAGTTT GAGGAGAGCC GGCTCATCCG GATGGCCGAG 
AGAGCCGAGT TCTACCAAAT TTGTGAGTTT ATGTATGAAC GAGAGCACCA GTACGACAAG 
ATCATCGACT GCTACCTCCG GGACCCCCTG AGAGAGGAAG AGGTGTTCAA CTACGTCCAC 
AACATCCTGT CCATTCCCGG GCACAGCCCC GAGGAGAAGC AGTCCGTGGG GCAAAAGGCC 
ATGCAGCACA TTGAGGAGCT CGTGTCTCTG AACCCCTGCA AAGCAGCAGA GCTGGTGGCC 
ACCCATTTCT CCGGGCAAGT TGAGGCGGTC ATCCAGAAAC TTCAGAACCC GTTTCTGCTC 
TTCCAGTTTC TGAGGAGCCT TCTTGACCCA AGGGAAGGAG TTTCCGTTAA CCAAGAGTCT 
CTGCACGCGT CCCCTTGCAT CACGGAGCAG TTCATTGAGC TGCTGTGTCA GTATAACCCT 
CACCAAGTGA TTGACACTCT GCAAGTCCTA GAGTCCTACC GGCTGGAAGA GACCATCCAG 
ATTACCCAGA AGTACCAGCT TCTTGAAGTA TGTGCCTACC TCTTAGAGAA GAAAGGAGAT 
GTTCATGGTG CTTTCTTAAT AATGCTCGAG AGACTACAAA GCAAGCTCCA GCAGTTTACA 
AAGGAAAGTG AAAACTCGAG AGAGAGCTCT TCCCTCAAGG CCGTGGAAGA GATCTTGGTG 
GAGACCATCG CCCTCTGCCA GAGGAATTCA CAGAGTCTGA ACCAGCCCCA GCGAGAGGCG 
CTCTGGTTTC CCCTCTTGGA GGCAATGATG GCCCCTCAGA AGCTGTCCAG CTCCTGCGCT 
CCCCCTCGCC TGGCAGAACC CTTGAAGTCT CTAACCATGC AGGTGCTGAA CAGCATGGCA 
GCCTTCATCG CTCTGCCGTC CATCTTGCAG CGAATCCTGC AGGATCCAGT TTATGGAAGA 
GGAAAACTTG GCGAGATCCA AGGACTGATC CTGGGAATGC TGGACACCTT TAACTACGAA 
CAAACTCTCC TGGAGACCAC CACCAGCCTC CTCAACCACG ACCTGCACTG GTCCCTCTGT 
AACCTGCGGG CATCGGTCAC CAGGGGGCTG AACCCAAAGC AGGATTACTG CTGCATCTGC 
CTGCAGCAGT ACCGGCGGCG CCAGGAGGTG GCCGACGACA TCGTCGTCTT CAGCTGCGGC 
CATCTCTACC ACTCCTTGTG CCTGCTGAAC AAAGAGTGTG CCGTGGGCAC GGGGAACCAG 
GCCCGCTGGA CGTGCTACAG ATGCAATTCA AGCAACAAGG CAGGGAAGCT CAGCGAAAAG 
GCATCTGAAG TGAAGAAGGG GCAGCTTGCC CAGGCCCAGG TGAAAATGTC TCCTTCCTGT 
CCATCGCCCC GAGGAGATGC TGCTGCGAAG AAGGCCCCGT CAGAACTGGG TCTGGACCCC 
CAGCAGATCC AGGCTTTCGA CCAGCTCTGC CGCCTTTACC GGGGAACCTC CAGGCTGGCG 
CTGCTGACCG AGCTGTCCCA GAACCAGAGC GGGGAGAGTC GCCGGCTGCT GGGGGGCTCC 
AAGAGCAGCC CCGCCCTCAA CAGCATCTTC CAGAACGAGA ACTTCCAGCT GCACCTGAGC 
CCTCCCCCCC TGAGCGAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS8 ( XM_031968080.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823940.1 Sarcophilus harrisii VPS8 subunit of CORVET complex (VPS8), transcript variant X1, mRNA.

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CloneID	OSa234978
Clone ID Related Accession (Same CDS sequence)	XM_031968081.1 , XM_031968080.1
Accession Version	XM_031968080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGTG AAGTCGATCC CGAGAGTGAG GAGGCAGCCC CCTCGCCCCG CGGAGGCCTC 
GGGGAAGGCC TGGCCCAGGC CCTGGAGGCT TCCCTGCCCA GGCTCACCTG CATAGACCTG 
GACAAGGACC TGGAGTTCAG AAATGAGCTG ATCGATGACA AGGAGTTTGA TATTCCTCAG 
GTGGACACCC CGCCCACCCT CGAAAGCATT CTCAATGAGA CCGATGATGA AGACGAATCC 
TTTGTTCTTG AGGATCCAAC GCTGTTAAAT ATCGACACCT TTGACACGCA CTCCTACGAC 
ACGTCGTCGG TGGCAAGCTC CGACAGCGGT GATCGAACCA ATCTGAAGAG GAAGAAGAAG 
CTTCTTGATT CTTTTTCTCT CCATGGCTCA GTCATGCGGC ATTCGCTTCT GAAGGGGATC 
TCTGCCCAGA TCGTCTCGGC AGCTGACAAA GTTGATGCCG GCTTGCCTAC TGCAATAGCT 
GTATCCAGTC TGATCGCAGT GGGGACTTCT CATGGATTGG CCTTAATCTT CGGAAAAGAT 
CAGAATCAAG CCCTGCGCCT CTGCTTGGGC AGCACCAGCG TCGGGGGACA GTACGGGGCC 
ATCTCTGCCC TCAGCATCAA CCACGACTGC TCCAGACTTC TCTGCGGCTT TGCCAAAGGC 
CAGATGACCA TGTGGGACTT GGCCAGCGGG AAGCTTTTGA GATCAATAAC AGATGCTCAC 
CCCCCAGGAA CAGCAATTCT GCACATTAAG TTCACAGATG ACCCAACTCT GGCCATTTGC 
AACGACAGCG GCGGTTCTGT GTTTGAGCTG ACATTTAAGC GGGTGATGGG AGTGAGGACG 
TGTGAATCCC GGTGTCTGTT CAGCGGGTCC AAGGGGGAAG TTTGCTGCAT CGAGCCTCTG 
CACTCCAAGG CCGAGCTGAA GGATCACCCC ATAACCCAGT TCTCCTTGCT GGCCATGGCG 
TCCCTAACTA AAATATTGGT CATTGGACTG AAGCCATCTC TGAAAGTGTG GATGACTTTC 
CCCTATGGAC GGATGGATCC TTCCAGCGTC CCGCTGCTGG CCTGGCATTT TGTGGCCGCC 
AATAACTACG TGAACCCCAC GCTGGCCTTT TGCCGAGGGG ACGTCGTTCA CTTCCTGTTG 
GTGAAGCGGG ATGAGTCGGG CGCCATACAC GTCACCAAGC AAAAGCACCT GCACCTCTAC 
TATGACCTCA TTAACTTCAC TTGGATCAAT TCCCGGACGA TCGTGCTCCT GGACAGTGTG 
GAGAAGCTGC ATGTGATCGA CCGGCTGACG CAGGAGGAGC TGGAGACGGT AGAAATCTCC 
GAGGTGCAGC TGGTCTACAA CAGTAGCCAC TTCAAGTCTC TGGCCACGGG TGGCAACGTC 
AGCCAGGCCC TGGCTCTGGT TGGAGAGAAA GCCTGTTACC AGTCCATCAG CAGTTATGGT 
GGCCAGATCT TTTATTTGGG GACAAAATCT GTTTATGTGA TGACGTTGAG GAGCTGGAGA 
GAGAGAGTGG ACCATCTCCT GAAACAAGAG TGTCTGCCGG AGGCCTTGGC TCTCGCGTGG 
TCCTTCCACG AAGGGAAGGC CAAAGCTGTC GTTGGCTTGT CTGGGGACGC CAGCAAGCGG 
AAGGCCGTCG TTGCCGATCG GATGGTTGAA ATCCTCTTCC ACTGTACGGA CCGAGCTTTG 
AAAAAATGCC CGGACCAGGG CAAGATCCAG GTGATGGAGC AGCATTTTCA GGACACGGTG 
CCCGTGGTTG TTGATTACTG CCTTCGGCTG CAGCGCAGGG ATCTCCTGTT TAGCCAAATG 
TACGACAAGT TCAGTGAGAA TTCGGTGGCC AAAGGAGTCT TCCTGGAGTG CCTGGAGCCG 
TACATTCTGA GTGACCAGCT GGTGGGGATC ACCGCCCAGG TGATGAAGGA TCTGCTCATT 
CACTTCCAAG ATAAAAAGCT GATGGAGAAT GTGGAGGCAC TGATCGTGCA CATGGACATC 
ACCAGCCTGG ACATCCAGCA GGTGGTTCTC ATGTGCTGGG AGAACCATCT CTACGACGCC 
ATGATCTACG TCTACAACAG CGGCATGAAC GAGTTCATCA GCCCCATGGA GAAACTTTTC 
AAGGTCATTG CTCCTCCTCT GAGCGCAGGG AAGTCCCTGA CAGATGAGCA GGTTATCATG 
GGCAACAAGC TTCTTGTGTA CATCAGCTGC TGCTTGGCGG GCCGGGCTTA CCCCCTGGGG 
GATATCCCTG AAGACCTGGT GCCCTTGGTT AAAAACCAGG TCTTCGAGTT TCTGATCCGC 
CTGCATTCTG CGGAAGCCTC CTCGGAGGAG GAGATCTACC CCTACATCTG CACCCTGCTT 
CACTTCGACA CCAGGGAGTT CCTGAACGTC CTGGCTCTGA CTTTTGAAGA TTTTAAAAAT 
GACAAGCAGG CTCTGGAATA CCAGCAGCGG ATCGTGGACA TCTTGCTGAA GGTCATGGTG 
GAAAATTCCG ACTTTACTCC TTCCCAAGTC GGGTGCCTCT TCACCTTCCT GGCCCGGCAG 
CTCGCCAAGC CCGACAACAC GCTCTTCGTG AACAGGACGC TTTTTGACCA GGTGCTCGAG 
TTCCTCTGCA GCCCCGATGA TGACTCCCGA CACTCGGAGA GGCAGCAGGT CCTCCTCGAG 
TTGCTCCAGG CCGGAGGGAT TGTGCAGTTT GAGGAGAGCC GGCTCATCCG GATGGCCGAG 
AGAGCCGAGT TCTACCAAAT TTGTGAGTTT ATGTATGAAC GAGAGCACCA GTACGACAAG 
ATCATCGACT GCTACCTCCG GGACCCCCTG AGAGAGGAAG AGGTGTTCAA CTACGTCCAC 
AACATCCTGT CCATTCCCGG GCACAGCCCC GAGGAGAAGC AGTCCGTGGG GCAAAAGGCC 
ATGCAGCACA TTGAGGAGCT CGTGTCTCTG AACCCCTGCA AAGCAGCAGA GCTGGTGGCC 
ACCCATTTCT CCGGGCAAGT TGAGGCGGTC ATCCAGAAAC TTCAGAACCC GTTTCTGCTC 
TTCCAGTTTC TGAGGAGCCT TCTTGACCCA AGGGAAGGAG TTTCCGTTAA CCAAGAGTCT 
CTGCACGCGT CCCCTTGCAT CACGGAGCAG TTCATTGAGC TGCTGTGTCA GTATAACCCT 
CACCAAGTGA TTGACACTCT GCAAGTCCTA GAGTCCTACC GGCTGGAAGA GACCATCCAG 
ATTACCCAGA AGTACCAGCT TCTTGAAGTA TGTGCCTACC TCTTAGAGAA GAAAGGAGAT 
GTTCATGGTG CTTTCTTAAT AATGCTCGAG AGACTACAAA GCAAGCTCCA GCAGTTTACA 
AAGGAAAGTG AAAACTCGAG AGAGAGCTCT TCCCTCAAGG CCGTGGAAGA GATCTTGGTG 
GAGACCATCG CCCTCTGCCA GAGGAATTCA CAGAGTCTGA ACCAGCCCCA GCGAGAGGCG 
CTCTGGTTTC CCCTCTTGGA GGCAATGATG GCCCCTCAGA AGCTGTCCAG CTCCTGCGCT 
CCCCCTCGCC TGGCAGAACC CTTGAAGTCT CTAACCATGC AGGTGCTGAA CAGCATGGCA 
GCCTTCATCG CTCTGCCGTC CATCTTGCAG CGAATCCTGC AGGATCCAGT TTATGGAAGA 
GGAAAACTTG GCGAGATCCA AGGACTGATC CTGGGAATGC TGGACACCTT TAACTACGAA 
CAAACTCTCC TGGAGACCAC CACCAGCCTC CTCAACCACG ACCTGCACTG GTCCCTCTGT 
AACCTGCGGG CATCGGTCAC CAGGGGGCTG AACCCAAAGC AGGATTACTG CTGCATCTGC 
CTGCAGCAGT ACCGGCGGCG CCAGGAGGTG GCCGACGACA TCGTCGTCTT CAGCTGCGGC 
CATCTCTACC ACTCCTTGTG CCTGCTGAAC AAAGAGTGTG CCGTGGGCAC GGGGAACCAG 
GCCCGCTGGA CGTGCTACAG ATGCAATTCA AGCAACAAGG CAGGGAAGCT CAGCGAAAAG 
GCATCTGAAG TGAAGAAGGG GCAGCTTGCC CAGGCCCAGG TGAAAATGTC TCCTTCCTGT 
CCATCGCCCC GAGGAGATGC TGCTGCGAAG AAGGCCCCGT CAGAACTGGG TCTGGACCCC 
CAGCAGATCC AGGCTTTCGA CCAGCTCTGC CGCCTTTACC GGGGAACCTC CAGGCTGGCG 
CTGCTGACCG AGCTGTCCCA GAACCAGAGC GGGGAGAGTC GCCGGCTGCT GGGGGGCTCC 
AAGAGCAGCC CCGCCCTCAA CAGCATCTTC CAGAACGAGA ACTTCCAGCT GCACCTGAGC 
CCTCCCCCCC TGAGCGAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823940.1
CDS	293..4576
Translation	MASEVDPESEEAAPSPRGGLGEGLAQALEASLPRLTCIDLDKDLEFRNELIDDKEFDIPQVDTPPTLESILNETDDEDESFVLEDPTLLNIDTFDTHSYDTSSVASSDSGDRTNLKRKKKLLDSFSLHGSVMRHSLLKGISAQIVSAADKVDAGLPTAIAVSSLIAVGTSHGLALIFGKDQNQALRLCLGSTSVGGQYGAISALSINHDCSRLLCGFAKGQMTMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRVMGVRTCESRCLFSGSKGEVCCIEPLHSKAELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAANNYVNPTLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRLTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMTLRSWRERVDHLLKQECLPEALALAWSFHEGKAKAVVGLSGDASKRKAVVADRMVEILFHCTDRALKKCPDQGKIQVMEQHFQDTVPVVVDYCLRLQRRDLLFSQMYDKFSENSVAKGVFLECLEPYILSDQLVGITAQVMKDLLIHFQDKKLMENVEALIVHMDITSLDIQQVVLMCWENHLYDAMIYVYNSGMNEFISPMEKLFKVIAPPLSAGKSLTDEQVIMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSAEASSEEEIYPYICTLLHFDTREFLNVLALTFEDFKNDKQALEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAERAEFYQICEFMYEREHQYDKIIDCYLRDPLREEEVFNYVHNILSIPGHSPEEKQSVGQKAMQHIEELVSLNPCKAAELVATHFSGQVEAVIQKLQNPFLLFQFLRSLLDPREGVSVNQESLHASPCITEQFIELLCQYNPHQVIDTLQVLESYRLEETIQITQKYQLLEVCAYLLEKKGDVHGAFLIMLERLQSKLQQFTKESENSRESSSLKAVEEILVETIALCQRNSQSLNQPQREALWFPLLEAMMAPQKLSSSCAPPRLAEPLKSLTMQVLNSMAAFIALPSILQRILQDPVYGRGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNHDLHWSLCNLRASVTRGLNPKQDYCCICLQQYRRRQEVADDIVVFSCGHLYHSLCLLNKECAVGTGNQARWTCYRCNSSNKAGKLSEKASEVKKGQLAQAQVKMSPSCPSPRGDAAAKKAPSELGLDPQQIQAFDQLCRLYRGTSRLALLTELSQNQSGESRRLLGGSKSSPALNSIFQNENFQLHLSPPPLSED

Target ORF information:

RefSeq Version	XM_031968080.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii VPS8 subunit of CORVET complex (VPS8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031968080.1

ORF Insert Sequence:

ATGGCGAGTG AAGTCGATCC CGAGAGTGAG GAGGCAGCCC CCTCGCCCCG CGGAGGCCTC 
GGGGAAGGCC TGGCCCAGGC CCTGGAGGCT TCCCTGCCCA GGCTCACCTG CATAGACCTG 
GACAAGGACC TGGAGTTCAG AAATGAGCTG ATCGATGACA AGGAGTTTGA TATTCCTCAG 
GTGGACACCC CGCCCACCCT CGAAAGCATT CTCAATGAGA CCGATGATGA AGACGAATCC 
TTTGTTCTTG AGGATCCAAC GCTGTTAAAT ATCGACACCT TTGACACGCA CTCCTACGAC 
ACGTCGTCGG TGGCAAGCTC CGACAGCGGT GATCGAACCA ATCTGAAGAG GAAGAAGAAG 
CTTCTTGATT CTTTTTCTCT CCATGGCTCA GTCATGCGGC ATTCGCTTCT GAAGGGGATC 
TCTGCCCAGA TCGTCTCGGC AGCTGACAAA GTTGATGCCG GCTTGCCTAC TGCAATAGCT 
GTATCCAGTC TGATCGCAGT GGGGACTTCT CATGGATTGG CCTTAATCTT CGGAAAAGAT 
CAGAATCAAG CCCTGCGCCT CTGCTTGGGC AGCACCAGCG TCGGGGGACA GTACGGGGCC 
ATCTCTGCCC TCAGCATCAA CCACGACTGC TCCAGACTTC TCTGCGGCTT TGCCAAAGGC 
CAGATGACCA TGTGGGACTT GGCCAGCGGG AAGCTTTTGA GATCAATAAC AGATGCTCAC 
CCCCCAGGAA CAGCAATTCT GCACATTAAG TTCACAGATG ACCCAACTCT GGCCATTTGC 
AACGACAGCG GCGGTTCTGT GTTTGAGCTG ACATTTAAGC GGGTGATGGG AGTGAGGACG 
TGTGAATCCC GGTGTCTGTT CAGCGGGTCC AAGGGGGAAG TTTGCTGCAT CGAGCCTCTG 
CACTCCAAGG CCGAGCTGAA GGATCACCCC ATAACCCAGT TCTCCTTGCT GGCCATGGCG 
TCCCTAACTA AAATATTGGT CATTGGACTG AAGCCATCTC TGAAAGTGTG GATGACTTTC 
CCCTATGGAC GGATGGATCC TTCCAGCGTC CCGCTGCTGG CCTGGCATTT TGTGGCCGCC 
AATAACTACG TGAACCCCAC GCTGGCCTTT TGCCGAGGGG ACGTCGTTCA CTTCCTGTTG 
GTGAAGCGGG ATGAGTCGGG CGCCATACAC GTCACCAAGC AAAAGCACCT GCACCTCTAC 
TATGACCTCA TTAACTTCAC TTGGATCAAT TCCCGGACGA TCGTGCTCCT GGACAGTGTG 
GAGAAGCTGC ATGTGATCGA CCGGCTGACG CAGGAGGAGC TGGAGACGGT AGAAATCTCC 
GAGGTGCAGC TGGTCTACAA CAGTAGCCAC TTCAAGTCTC TGGCCACGGG TGGCAACGTC 
AGCCAGGCCC TGGCTCTGGT TGGAGAGAAA GCCTGTTACC AGTCCATCAG CAGTTATGGT 
GGCCAGATCT TTTATTTGGG GACAAAATCT GTTTATGTGA TGACGTTGAG GAGCTGGAGA 
GAGAGAGTGG ACCATCTCCT GAAACAAGAG TGTCTGCCGG AGGCCTTGGC TCTCGCGTGG 
TCCTTCCACG AAGGGAAGGC CAAAGCTGTC GTTGGCTTGT CTGGGGACGC CAGCAAGCGG 
AAGGCCGTCG TTGCCGATCG GATGGTTGAA ATCCTCTTCC ACTGTACGGA CCGAGCTTTG 
AAAAAATGCC CGGACCAGGG CAAGATCCAG GTGATGGAGC AGCATTTTCA GGACACGGTG 
CCCGTGGTTG TTGATTACTG CCTTCGGCTG CAGCGCAGGG ATCTCCTGTT TAGCCAAATG 
TACGACAAGT TCAGTGAGAA TTCGGTGGCC AAAGGAGTCT TCCTGGAGTG CCTGGAGCCG 
TACATTCTGA GTGACCAGCT GGTGGGGATC ACCGCCCAGG TGATGAAGGA TCTGCTCATT 
CACTTCCAAG ATAAAAAGCT GATGGAGAAT GTGGAGGCAC TGATCGTGCA CATGGACATC 
ACCAGCCTGG ACATCCAGCA GGTGGTTCTC ATGTGCTGGG AGAACCATCT CTACGACGCC 
ATGATCTACG TCTACAACAG CGGCATGAAC GAGTTCATCA GCCCCATGGA GAAACTTTTC 
AAGGTCATTG CTCCTCCTCT GAGCGCAGGG AAGTCCCTGA CAGATGAGCA GGTTATCATG 
GGCAACAAGC TTCTTGTGTA CATCAGCTGC TGCTTGGCGG GCCGGGCTTA CCCCCTGGGG 
GATATCCCTG AAGACCTGGT GCCCTTGGTT AAAAACCAGG TCTTCGAGTT TCTGATCCGC 
CTGCATTCTG CGGAAGCCTC CTCGGAGGAG GAGATCTACC CCTACATCTG CACCCTGCTT 
CACTTCGACA CCAGGGAGTT CCTGAACGTC CTGGCTCTGA CTTTTGAAGA TTTTAAAAAT 
GACAAGCAGG CTCTGGAATA CCAGCAGCGG ATCGTGGACA TCTTGCTGAA GGTCATGGTG 
GAAAATTCCG ACTTTACTCC TTCCCAAGTC GGGTGCCTCT TCACCTTCCT GGCCCGGCAG 
CTCGCCAAGC CCGACAACAC GCTCTTCGTG AACAGGACGC TTTTTGACCA GGTGCTCGAG 
TTCCTCTGCA GCCCCGATGA TGACTCCCGA CACTCGGAGA GGCAGCAGGT CCTCCTCGAG 
TTGCTCCAGG CCGGAGGGAT TGTGCAGTTT GAGGAGAGCC GGCTCATCCG GATGGCCGAG 
AGAGCCGAGT TCTACCAAAT TTGTGAGTTT ATGTATGAAC GAGAGCACCA GTACGACAAG 
ATCATCGACT GCTACCTCCG GGACCCCCTG AGAGAGGAAG AGGTGTTCAA CTACGTCCAC 
AACATCCTGT CCATTCCCGG GCACAGCCCC GAGGAGAAGC AGTCCGTGGG GCAAAAGGCC 
ATGCAGCACA TTGAGGAGCT CGTGTCTCTG AACCCCTGCA AAGCAGCAGA GCTGGTGGCC 
ACCCATTTCT CCGGGCAAGT TGAGGCGGTC ATCCAGAAAC TTCAGAACCC GTTTCTGCTC 
TTCCAGTTTC TGAGGAGCCT TCTTGACCCA AGGGAAGGAG TTTCCGTTAA CCAAGAGTCT 
CTGCACGCGT CCCCTTGCAT CACGGAGCAG TTCATTGAGC TGCTGTGTCA GTATAACCCT 
CACCAAGTGA TTGACACTCT GCAAGTCCTA GAGTCCTACC GGCTGGAAGA GACCATCCAG 
ATTACCCAGA AGTACCAGCT TCTTGAAGTA TGTGCCTACC TCTTAGAGAA GAAAGGAGAT 
GTTCATGGTG CTTTCTTAAT AATGCTCGAG AGACTACAAA GCAAGCTCCA GCAGTTTACA 
AAGGAAAGTG AAAACTCGAG AGAGAGCTCT TCCCTCAAGG CCGTGGAAGA GATCTTGGTG 
GAGACCATCG CCCTCTGCCA GAGGAATTCA CAGAGTCTGA ACCAGCCCCA GCGAGAGGCG 
CTCTGGTTTC CCCTCTTGGA GGCAATGATG GCCCCTCAGA AGCTGTCCAG CTCCTGCGCT 
CCCCCTCGCC TGGCAGAACC CTTGAAGTCT CTAACCATGC AGGTGCTGAA CAGCATGGCA 
GCCTTCATCG CTCTGCCGTC CATCTTGCAG CGAATCCTGC AGGATCCAGT TTATGGAAGA 
GGAAAACTTG GCGAGATCCA AGGACTGATC CTGGGAATGC TGGACACCTT TAACTACGAA 
CAAACTCTCC TGGAGACCAC CACCAGCCTC CTCAACCACG ACCTGCACTG GTCCCTCTGT 
AACCTGCGGG CATCGGTCAC CAGGGGGCTG AACCCAAAGC AGGATTACTG CTGCATCTGC 
CTGCAGCAGT ACCGGCGGCG CCAGGAGGTG GCCGACGACA TCGTCGTCTT CAGCTGCGGC 
CATCTCTACC ACTCCTTGTG CCTGCTGAAC AAAGAGTGTG CCGTGGGCAC GGGGAACCAG 
GCCCGCTGGA CGTGCTACAG ATGCAATTCA AGCAACAAGG CAGGGAAGCT CAGCGAAAAG 
GCATCTGAAG TGAAGAAGGG GCAGCTTGCC CAGGCCCAGG TGAAAATGTC TCCTTCCTGT 
CCATCGCCCC GAGGAGATGC TGCTGCGAAG AAGGCCCCGT CAGAACTGGG TCTGGACCCC 
CAGCAGATCC AGGCTTTCGA CCAGCTCTGC CGCCTTTACC GGGGAACCTC CAGGCTGGCG 
CTGCTGACCG AGCTGTCCCA GAACCAGAGC GGGGAGAGTC GCCGGCTGCT GGGGGGCTCC 
AAGAGCAGCC CCGCCCTCAA CAGCATCTTC CAGAACGAGA ACTTCCAGCT GCACCTGAGC 
CCTCCCCCCC TGAGCGAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS8 ( XM_031968082.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823942.1 Sarcophilus harrisii VPS8 subunit of CORVET complex (VPS8), transcript variant X3, mRNA.

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CloneID	OSa234979
Clone ID Related Accession (Same CDS sequence)	XM_031968082.1
Accession Version	XM_031968082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	vacuolar protein sorting-associated protein 8 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGTG AAGTCGATCC CGAGAGTGAG GAGGCAGCCC CCTCGCCCCG CGGAGGCCTC 
GGGGAAGGCC TGGCCCAGGC CCTGGAGGCT TCCCTGCCCA GGCTCACCTG CATAGACCTG 
GACAAGGACC TGGAGTTCAG AAATGAGCTG ATCGATGACA AGGAGTTTGA TATTCCTCAG 
GTGGACACCC CGCCCACCCT CGAAAGCATT CTCAATGAGA CCGATGATGA AGACGAATCC 
TTTGTTCTTG AGGATCCAAC GCTGTTAAAT ATCGACACCT TTGACACGCA CTCCTACGAC 
ACGTCGTCGG TGGCAAGCTC CGACAGCGGT GATCGAACCA ATCTGAAGAG GAAGAAGAAG 
CTTCTTGATT CTTTTTCTCT CCATGGCTCA GTCATGCGGC ATTCGCTTCT GAAGGGGATC 
TCTGCCCAGA TCGTCTCGGC AGCTGACAAA GTTGATGCCG GCTTGCCTAC TGCAATAGCT 
GTATCCAGTC TGATCGCAGT GGGGACTTCT CATGGATTGG CCTTAATCTT CGATCAGAAT 
CAAGCCCTGC GCCTCTGCTT GGGCAGCACC AGCGTCGGGG GACAGTACGG GGCCATCTCT 
GCCCTCAGCA TCAACCACGA CTGCTCCAGA CTTCTCTGCG GCTTTGCCAA AGGCCAGATG 
ACCATGTGGG ACTTGGCCAG CGGGAAGCTT TTGAGATCAA TAACAGATGC TCACCCCCCA 
GGAACAGCAA TTCTGCACAT TAAGTTCACA GATGACCCAA CTCTGGCCAT TTGCAACGAC 
AGCGGCGGTT CTGTGTTTGA GCTGACATTT AAGCGGGTGA TGGGAGTGAG GACGTGTGAA 
TCCCGGTGTC TGTTCAGCGG GTCCAAGGGG GAAGTTTGCT GCATCGAGCC TCTGCACTCC 
AAGGCCGAGC TGAAGGATCA CCCCATAACC CAGTTCTCCT TGCTGGCCAT GGCGTCCCTA 
ACTAAAATAT TGGTCATTGG ACTGAAGCCA TCTCTGAAAG TGTGGATGAC TTTCCCCTAT 
GGACGGATGG ATCCTTCCAG CGTCCCGCTG CTGGCCTGGC ATTTTGTGGC CGCCAATAAC 
TACGTGAACC CCACGCTGGC CTTTTGCCGA GGGGACGTCG TTCACTTCCT GTTGGTGAAG 
CGGGATGAGT CGGGCGCCAT ACACGTCACC AAGCAAAAGC ACCTGCACCT CTACTATGAC 
CTCATTAACT TCACTTGGAT CAATTCCCGG ACGATCGTGC TCCTGGACAG TGTGGAGAAG 
CTGCATGTGA TCGACCGGCT GACGCAGGAG GAGCTGGAGA CGGTAGAAAT CTCCGAGGTG 
CAGCTGGTCT ACAACAGTAG CCACTTCAAG TCTCTGGCCA CGGGTGGCAA CGTCAGCCAG 
GCCCTGGCTC TGGTTGGAGA GAAAGCCTGT TACCAGTCCA TCAGCAGTTA TGGTGGCCAG 
ATCTTTTATT TGGGGACAAA ATCTGTTTAT GTGATGACGT TGAGGAGCTG GAGAGAGAGA 
GTGGACCATC TCCTGAAACA AGAGTGTCTG CCGGAGGCCT TGGCTCTCGC GTGGTCCTTC 
CACGAAGGGA AGGCCAAAGC TGTCGTTGGC TTGTCTGGGG ACGCCAGCAA GCGGAAGGCC 
GTCGTTGCCG ATCGGATGGT TGAAATCCTC TTCCACTGTA CGGACCGAGC TTTGAAAAAA 
TGCCCGGACC AGGGCAAGAT CCAGGTGATG GAGCAGCATT TTCAGGACAC GGTGCCCGTG 
GTTGTTGATT ACTGCCTTCG GCTGCAGCGC AGGGATCTCC TGTTTAGCCA AATGTACGAC 
AAGTTCAGTG AGAATTCGGT GGCCAAAGGA GTCTTCCTGG AGTGCCTGGA GCCGTACATT 
CTGAGTGACC AGCTGGTGGG GATCACCGCC CAGGTGATGA AGGATCTGCT CATTCACTTC 
CAAGATAAAA AGCTGATGGA GAATGTGGAG GCACTGATCG TGCACATGGA CATCACCAGC 
CTGGACATCC AGCAGGTGGT TCTCATGTGC TGGGAGAACC ATCTCTACGA CGCCATGATC 
TACGTCTACA ACAGCGGCAT GAACGAGTTC ATCAGCCCCA TGGAGAAACT TTTCAAGGTC 
ATTGCTCCTC CTCTGAGCGC AGGGAAGTCC CTGACAGATG AGCAGGTTAT CATGGGCAAC 
AAGCTTCTTG TGTACATCAG CTGCTGCTTG GCGGGCCGGG CTTACCCCCT GGGGGATATC 
CCTGAAGACC TGGTGCCCTT GGTTAAAAAC CAGGTCTTCG AGTTTCTGAT CCGCCTGCAT 
TCTGCGGAAG CCTCCTCGGA GGAGGAGATC TACCCCTACA TCTGCACCCT GCTTCACTTC 
GACACCAGGG AGTTCCTGAA CGTCCTGGCT CTGACTTTTG AAGATTTTAA AAATGACAAG 
CAGGCTCTGG AATACCAGCA GCGGATCGTG GACATCTTGC TGAAGGTCAT GGTGGAAAAT 
TCCGACTTTA CTCCTTCCCA AGTCGGGTGC CTCTTCACCT TCCTGGCCCG GCAGCTCGCC 
AAGCCCGACA ACACGCTCTT CGTGAACAGG ACGCTTTTTG ACCAGGTGCT CGAGTTCCTC 
TGCAGCCCCG ATGATGACTC CCGACACTCG GAGAGGCAGC AGGTCCTCCT CGAGTTGCTC 
CAGGCCGGAG GGATTGTGCA GTTTGAGGAG AGCCGGCTCA TCCGGATGGC CGAGAGAGCC 
GAGTTCTACC AAATTTGTGA GTTTATGTAT GAACGAGAGC ACCAGTACGA CAAGATCATC 
GACTGCTACC TCCGGGACCC CCTGAGAGAG GAAGAGGTGT TCAACTACGT CCACAACATC 
CTGTCCATTC CCGGGCACAG CCCCGAGGAG AAGCAGTCCG TGGGGCAAAA GGCCATGCAG 
CACATTGAGG AGCTCGTGTC TCTGAACCCC TGCAAAGCAG CAGAGCTGGT GGCCACCCAT 
TTCTCCGGGC AAGTTGAGGC GGTCATCCAG AAACTTCAGA ACCCGTTTCT GCTCTTCCAG 
TTTCTGAGGA GCCTTCTTGA CCCAAGGGAA GGAGTTTCCG TTAACCAAGA GTCTCTGCAC 
GCGTCCCCTT GCATCACGGA GCAGTTCATT GAGCTGCTGT GTCAGTATAA CCCTCACCAA 
GTGATTGACA CTCTGCAAGT CCTAGAGTCC TACCGGCTGG AAGAGACCAT CCAGATTACC 
CAGAAGTACC AGCTTCTTGA AGTATGTGCC TACCTCTTAG AGAAGAAAGG AGATGTTCAT 
GGTGCTTTCT TAATAATGCT CGAGAGACTA CAAAGCAAGC TCCAGCAGTT TACAAAGGAA 
AGTGAAAACT CGAGAGAGAG CTCTTCCCTC AAGGCCGTGG AAGAGATCTT GGTGGAGACC 
ATCGCCCTCT GCCAGAGGAA TTCACAGAGT CTGAACCAGC CCCAGCGAGA GGCGCTCTGG 
TTTCCCCTCT TGGAGGCAAT GATGGCCCCT CAGAAGCTGT CCAGCTCCTG CGCTCCCCCT 
CGCCTGGCAG AACCCTTGAA GTCTCTAACC ATGCAGGTGC TGAACAGCAT GGCAGCCTTC 
ATCGCTCTGC CGTCCATCTT GCAGCGAATC CTGCAGGATC CAGTTTATGG AAGAGGAAAA 
CTTGGCGAGA TCCAAGGACT GATCCTGGGA ATGCTGGACA CCTTTAACTA CGAACAAACT 
CTCCTGGAGA CCACCACCAG CCTCCTCAAC CACGACCTGC ACTGGTCCCT CTGTAACCTG 
CGGGCATCGG TCACCAGGGG GCTGAACCCA AAGCAGGATT ACTGCTGCAT CTGCCTGCAG 
CAGTACCGGC GGCGCCAGGA GGTGGCCGAC GACATCGTCG TCTTCAGCTG CGGCCATCTC 
TACCACTCCT TGTGCCTGCT GAACAAAGAG TGTGCCGTGG GCACGGGGAA CCAGGCCCGC 
TGGACGTGCT ACAGATGCAA TTCAAGCAAC AAGGCAGGGA AGCTCAGCGA AAAGGCATCT 
GAAGTGAAGA AGGGGCAGCT TGCCCAGGCC CAGGTGAAAA TGTCTCCTTC CTGTCCATCG 
CCCCGAGGAG ATGCTGCTGC GAAGAAGGCC CCGTCAGAAC TGGGTCTGGA CCCCCAGCAG 
ATCCAGGCTT TCGACCAGCT CTGCCGCCTT TACCGGGGAA CCTCCAGGCT GGCGCTGCTG 
ACCGAGCTGT CCCAGAACCA GAGCGGGGAG AGTCGCCGGC TGCTGGGGGG CTCCAAGAGC 
AGCCCCGCCC TCAACAGCAT CTTCCAGAAC GAGAACTTCC AGCTGCACCT GAGCCCTCCC 
CCCCTGAGCG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823942.1
CDS	292..4569
Translation	MASEVDPESEEAAPSPRGGLGEGLAQALEASLPRLTCIDLDKDLEFRNELIDDKEFDIPQVDTPPTLESILNETDDEDESFVLEDPTLLNIDTFDTHSYDTSSVASSDSGDRTNLKRKKKLLDSFSLHGSVMRHSLLKGISAQIVSAADKVDAGLPTAIAVSSLIAVGTSHGLALIFDQNQALRLCLGSTSVGGQYGAISALSINHDCSRLLCGFAKGQMTMWDLASGKLLRSITDAHPPGTAILHIKFTDDPTLAICNDSGGSVFELTFKRVMGVRTCESRCLFSGSKGEVCCIEPLHSKAELKDHPITQFSLLAMASLTKILVIGLKPSLKVWMTFPYGRMDPSSVPLLAWHFVAANNYVNPTLAFCRGDVVHFLLVKRDESGAIHVTKQKHLHLYYDLINFTWINSRTIVLLDSVEKLHVIDRLTQEELETVEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMTLRSWRERVDHLLKQECLPEALALAWSFHEGKAKAVVGLSGDASKRKAVVADRMVEILFHCTDRALKKCPDQGKIQVMEQHFQDTVPVVVDYCLRLQRRDLLFSQMYDKFSENSVAKGVFLECLEPYILSDQLVGITAQVMKDLLIHFQDKKLMENVEALIVHMDITSLDIQQVVLMCWENHLYDAMIYVYNSGMNEFISPMEKLFKVIAPPLSAGKSLTDEQVIMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSAEASSEEEIYPYICTLLHFDTREFLNVLALTFEDFKNDKQALEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKPDNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAERAEFYQICEFMYEREHQYDKIIDCYLRDPLREEEVFNYVHNILSIPGHSPEEKQSVGQKAMQHIEELVSLNPCKAAELVATHFSGQVEAVIQKLQNPFLLFQFLRSLLDPREGVSVNQESLHASPCITEQFIELLCQYNPHQVIDTLQVLESYRLEETIQITQKYQLLEVCAYLLEKKGDVHGAFLIMLERLQSKLQQFTKESENSRESSSLKAVEEILVETIALCQRNSQSLNQPQREALWFPLLEAMMAPQKLSSSCAPPRLAEPLKSLTMQVLNSMAAFIALPSILQRILQDPVYGRGKLGEIQGLILGMLDTFNYEQTLLETTTSLLNHDLHWSLCNLRASVTRGLNPKQDYCCICLQQYRRRQEVADDIVVFSCGHLYHSLCLLNKECAVGTGNQARWTCYRCNSSNKAGKLSEKASEVKKGQLAQAQVKMSPSCPSPRGDAAAKKAPSELGLDPQQIQAFDQLCRLYRGTSRLALLTELSQNQSGESRRLLGGSKSSPALNSIFQNENFQLHLSPPPLSED

Target ORF information:

RefSeq Version	XM_031968082.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii VPS8 subunit of CORVET complex (VPS8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031968082.1

ORF Insert Sequence:

ATGGCGAGTG AAGTCGATCC CGAGAGTGAG GAGGCAGCCC CCTCGCCCCG CGGAGGCCTC 
GGGGAAGGCC TGGCCCAGGC CCTGGAGGCT TCCCTGCCCA GGCTCACCTG CATAGACCTG 
GACAAGGACC TGGAGTTCAG AAATGAGCTG ATCGATGACA AGGAGTTTGA TATTCCTCAG 
GTGGACACCC CGCCCACCCT CGAAAGCATT CTCAATGAGA CCGATGATGA AGACGAATCC 
TTTGTTCTTG AGGATCCAAC GCTGTTAAAT ATCGACACCT TTGACACGCA CTCCTACGAC 
ACGTCGTCGG TGGCAAGCTC CGACAGCGGT GATCGAACCA ATCTGAAGAG GAAGAAGAAG 
CTTCTTGATT CTTTTTCTCT CCATGGCTCA GTCATGCGGC ATTCGCTTCT GAAGGGGATC 
TCTGCCCAGA TCGTCTCGGC AGCTGACAAA GTTGATGCCG GCTTGCCTAC TGCAATAGCT 
GTATCCAGTC TGATCGCAGT GGGGACTTCT CATGGATTGG CCTTAATCTT CGATCAGAAT 
CAAGCCCTGC GCCTCTGCTT GGGCAGCACC AGCGTCGGGG GACAGTACGG GGCCATCTCT 
GCCCTCAGCA TCAACCACGA CTGCTCCAGA CTTCTCTGCG GCTTTGCCAA AGGCCAGATG 
ACCATGTGGG ACTTGGCCAG CGGGAAGCTT TTGAGATCAA TAACAGATGC TCACCCCCCA 
GGAACAGCAA TTCTGCACAT TAAGTTCACA GATGACCCAA CTCTGGCCAT TTGCAACGAC 
AGCGGCGGTT CTGTGTTTGA GCTGACATTT AAGCGGGTGA TGGGAGTGAG GACGTGTGAA 
TCCCGGTGTC TGTTCAGCGG GTCCAAGGGG GAAGTTTGCT GCATCGAGCC TCTGCACTCC 
AAGGCCGAGC TGAAGGATCA CCCCATAACC CAGTTCTCCT TGCTGGCCAT GGCGTCCCTA 
ACTAAAATAT TGGTCATTGG ACTGAAGCCA TCTCTGAAAG TGTGGATGAC TTTCCCCTAT 
GGACGGATGG ATCCTTCCAG CGTCCCGCTG CTGGCCTGGC ATTTTGTGGC CGCCAATAAC 
TACGTGAACC CCACGCTGGC CTTTTGCCGA GGGGACGTCG TTCACTTCCT GTTGGTGAAG 
CGGGATGAGT CGGGCGCCAT ACACGTCACC AAGCAAAAGC ACCTGCACCT CTACTATGAC 
CTCATTAACT TCACTTGGAT CAATTCCCGG ACGATCGTGC TCCTGGACAG TGTGGAGAAG 
CTGCATGTGA TCGACCGGCT GACGCAGGAG GAGCTGGAGA CGGTAGAAAT CTCCGAGGTG 
CAGCTGGTCT ACAACAGTAG CCACTTCAAG TCTCTGGCCA CGGGTGGCAA CGTCAGCCAG 
GCCCTGGCTC TGGTTGGAGA GAAAGCCTGT TACCAGTCCA TCAGCAGTTA TGGTGGCCAG 
ATCTTTTATT TGGGGACAAA ATCTGTTTAT GTGATGACGT TGAGGAGCTG GAGAGAGAGA 
GTGGACCATC TCCTGAAACA AGAGTGTCTG CCGGAGGCCT TGGCTCTCGC GTGGTCCTTC 
CACGAAGGGA AGGCCAAAGC TGTCGTTGGC TTGTCTGGGG ACGCCAGCAA GCGGAAGGCC 
GTCGTTGCCG ATCGGATGGT TGAAATCCTC TTCCACTGTA CGGACCGAGC TTTGAAAAAA 
TGCCCGGACC AGGGCAAGAT CCAGGTGATG GAGCAGCATT TTCAGGACAC GGTGCCCGTG 
GTTGTTGATT ACTGCCTTCG GCTGCAGCGC AGGGATCTCC TGTTTAGCCA AATGTACGAC 
AAGTTCAGTG AGAATTCGGT GGCCAAAGGA GTCTTCCTGG AGTGCCTGGA GCCGTACATT 
CTGAGTGACC AGCTGGTGGG GATCACCGCC CAGGTGATGA AGGATCTGCT CATTCACTTC 
CAAGATAAAA AGCTGATGGA GAATGTGGAG GCACTGATCG TGCACATGGA CATCACCAGC 
CTGGACATCC AGCAGGTGGT TCTCATGTGC TGGGAGAACC ATCTCTACGA CGCCATGATC 
TACGTCTACA ACAGCGGCAT GAACGAGTTC ATCAGCCCCA TGGAGAAACT TTTCAAGGTC 
ATTGCTCCTC CTCTGAGCGC AGGGAAGTCC CTGACAGATG AGCAGGTTAT CATGGGCAAC 
AAGCTTCTTG TGTACATCAG CTGCTGCTTG GCGGGCCGGG CTTACCCCCT GGGGGATATC 
CCTGAAGACC TGGTGCCCTT GGTTAAAAAC CAGGTCTTCG AGTTTCTGAT CCGCCTGCAT 
TCTGCGGAAG CCTCCTCGGA GGAGGAGATC TACCCCTACA TCTGCACCCT GCTTCACTTC 
GACACCAGGG AGTTCCTGAA CGTCCTGGCT CTGACTTTTG AAGATTTTAA AAATGACAAG 
CAGGCTCTGG AATACCAGCA GCGGATCGTG GACATCTTGC TGAAGGTCAT GGTGGAAAAT 
TCCGACTTTA CTCCTTCCCA AGTCGGGTGC CTCTTCACCT TCCTGGCCCG GCAGCTCGCC 
AAGCCCGACA ACACGCTCTT CGTGAACAGG ACGCTTTTTG ACCAGGTGCT CGAGTTCCTC 
TGCAGCCCCG ATGATGACTC CCGACACTCG GAGAGGCAGC AGGTCCTCCT CGAGTTGCTC 
CAGGCCGGAG GGATTGTGCA GTTTGAGGAG AGCCGGCTCA TCCGGATGGC CGAGAGAGCC 
GAGTTCTACC AAATTTGTGA GTTTATGTAT GAACGAGAGC ACCAGTACGA CAAGATCATC 
GACTGCTACC TCCGGGACCC CCTGAGAGAG GAAGAGGTGT TCAACTACGT CCACAACATC 
CTGTCCATTC CCGGGCACAG CCCCGAGGAG AAGCAGTCCG TGGGGCAAAA GGCCATGCAG 
CACATTGAGG AGCTCGTGTC TCTGAACCCC TGCAAAGCAG CAGAGCTGGT GGCCACCCAT 
TTCTCCGGGC AAGTTGAGGC GGTCATCCAG AAACTTCAGA ACCCGTTTCT GCTCTTCCAG 
TTTCTGAGGA GCCTTCTTGA CCCAAGGGAA GGAGTTTCCG TTAACCAAGA GTCTCTGCAC 
GCGTCCCCTT GCATCACGGA GCAGTTCATT GAGCTGCTGT GTCAGTATAA CCCTCACCAA 
GTGATTGACA CTCTGCAAGT CCTAGAGTCC TACCGGCTGG AAGAGACCAT CCAGATTACC 
CAGAAGTACC AGCTTCTTGA AGTATGTGCC TACCTCTTAG AGAAGAAAGG AGATGTTCAT 
GGTGCTTTCT TAATAATGCT CGAGAGACTA CAAAGCAAGC TCCAGCAGTT TACAAAGGAA 
AGTGAAAACT CGAGAGAGAG CTCTTCCCTC AAGGCCGTGG AAGAGATCTT GGTGGAGACC 
ATCGCCCTCT GCCAGAGGAA TTCACAGAGT CTGAACCAGC CCCAGCGAGA GGCGCTCTGG 
TTTCCCCTCT TGGAGGCAAT GATGGCCCCT CAGAAGCTGT CCAGCTCCTG CGCTCCCCCT 
CGCCTGGCAG AACCCTTGAA GTCTCTAACC ATGCAGGTGC TGAACAGCAT GGCAGCCTTC 
ATCGCTCTGC CGTCCATCTT GCAGCGAATC CTGCAGGATC CAGTTTATGG AAGAGGAAAA 
CTTGGCGAGA TCCAAGGACT GATCCTGGGA ATGCTGGACA CCTTTAACTA CGAACAAACT 
CTCCTGGAGA CCACCACCAG CCTCCTCAAC CACGACCTGC ACTGGTCCCT CTGTAACCTG 
CGGGCATCGG TCACCAGGGG GCTGAACCCA AAGCAGGATT ACTGCTGCAT CTGCCTGCAG 
CAGTACCGGC GGCGCCAGGA GGTGGCCGAC GACATCGTCG TCTTCAGCTG CGGCCATCTC 
TACCACTCCT TGTGCCTGCT GAACAAAGAG TGTGCCGTGG GCACGGGGAA CCAGGCCCGC 
TGGACGTGCT ACAGATGCAA TTCAAGCAAC AAGGCAGGGA AGCTCAGCGA AAAGGCATCT 
GAAGTGAAGA AGGGGCAGCT TGCCCAGGCC CAGGTGAAAA TGTCTCCTTC CTGTCCATCG 
CCCCGAGGAG ATGCTGCTGC GAAGAAGGCC CCGTCAGAAC TGGGTCTGGA CCCCCAGCAG 
ATCCAGGCTT TCGACCAGCT CTGCCGCCTT TACCGGGGAA CCTCCAGGCT GGCGCTGCTG 
ACCGAGCTGT CCCAGAACCA GAGCGGGGAG AGTCGCCGGC TGCTGGGGGG CTCCAAGAGC 
AGCCCCGCCC TCAACAGCAT CTTCCAGAAC GAGAACTTCC AGCTGCACCT GAGCCCTCCC 
CCCCTGAGCG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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VPS8 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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