LOC100932056 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100932056
Entrez Gene ID	100932056
Full Name	centrosome-associated protein 350-like
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031954951.1	XP_031810811.1	coiled-coil domain-containing protein 187 isoform X2
XM_031954950.1	XP_031810810.1	coiled-coil domain-containing protein 187 isoform X1
XM_023494340.1	XP_023350108.1	LOW QUALITY PROTEIN: centrosome-associated protein 350-like
XM_012554016.1	XP_012409470.1	LOW QUALITY PROTEIN: centrosome-associated protein 350-like

Pathways from BioSystems
Gene Ontology

The following LOC100932056 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100932056 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa119088	XM_012554016.1 Latest version!	Sarcophilus harrisii centrosome-associated protein 350-like (LOC100932056), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa150401	XM_023494340.1 Latest version!	Sarcophilus harrisii centrosome-associated protein 350-like (LOC100932056), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa226575	XM_031954950.1 Latest version!	Sarcophilus harrisii coiled-coil domain containing 187 (CCDC187), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa226576	XM_031954951.1 Latest version!	Sarcophilus harrisii coiled-coil domain containing 187 (CCDC187), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100932056 ( XM_012554016.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012409470.1 Sarcophilus harrisii centrosome-associated protein 350-like (LOC100932056), mRNA.

Price & Availability↑

CloneID	OSa119088
Clone ID Related Accession (Same CDS sequence)	XM_012554016.1
Accession Version	XM_012554016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	LOW QUALITY PROTEIN: centrosome-associated protein 350-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816666.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 8% of CDS bases internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGGTATTTG ACTACATGAC CTCCAATGTT CCTCTCAGTT CTTTGAGCTC CTCCTCTCCA 
TCTGAACAGA TCCAATCAAG GCAGAAAAAC TCTGTCTACC TGAGCCCTCA GCATCTGCAT 
ATCATTTCCC CAGATGTGGC CTTGGCTTGG GTCAGCCTTC AAAGAGCCAG GCACGTGTTA 
CAGCGGATGG AGAGTGAAAT GGACACCCCC AGCTGGCACA GCATCAGCAG CCAGAGCTGC 
GGATCACTTG ATTTTGGGCA AGAGAAGCTG TTTTCTTGGG AAAACTGGCA CAATGCTAGT 
CCTGCCTATG CCAAACTGAG AATGCCTCAT TTTTCTGAGG ACTACAACAT GGCAGCTGCA 
TCAGTGTCAC CCTGGGTCTT TCGGGAGTCA GTTATCTTCA GAGAAGAACC AGATGGCAAG 
ACTTACGGTG GACGAGTAAC TCATTTGAAT CCTGTGATTT TTGGCCCAGT GTGGAAAGAG 
CAGGACTTTA ACTACCCACC TGAACCAGAG AGAATGTGTA CAACTAGTGA CTGGCCCTCC 
AAAATGCCCG TCTTTATTCC CGTTCAGTCG GGAAGCCATC ATCAGTTCTT GAATCACCCA 
AGTGACCCCT GGAGGGAAAA GTCACCTCAG CCAGTTGAGT CTCCCACTAA TTTTCAGAGA 
TGGCCATTGA CAGGGGTAAA GAGAGCTTTC CCTGAATCCC AGCTGGAGCA GCTGAGGGAG 
AAGATCCGGG CACAGAAACA TCGACATGCT AATGTTTTCC CTACCTCCTT CCAAGCTCGC 
TCTGGGACCC CCCAAACTCT GCCCTGGAAG CACGTGCCAA AGAGAAGCGT CTCCAAAATG 
AAGTTAGCTG CTTCAAAGCC AGCCCACCCG GGCCAAGTCT CGGCTTCCAG CCCAGATCCC 
TCTGACACTC ACCATGCCGT TTCCCACAAA TTTATACCAA AGAGAAAGAG CTACAGAGTT 
AGATTTGCAT TATCAACGCC AATAGACGCA CATCAAAGGG ATAAAAATAA AACTACAGTG 
ACCAAAAGCT GCCAGGTATC TTCTCAGTCA CTTTCTCTTG GAAAACAGCT AAAAGGCCAA 
GATGCTAAGG TGACTGGCGT TTCTGCATGG AGAAAGGGAC AGAGGCTTGC GAGGCTCCTC 
CTTGGGCCCC CTCCCACGTT TCTCCAGCTC CAGAAAAGGG CTCTCTCCGG AGGCCTGGCC 
GCCACAAAGG AGTCAGGACT GACAGAGAAG ACTCAAAAAT CTGAGCACAT ACCACCCTCC 
ACCCGAGATA TAAACAACCA GAAAGGAGCA GCTCCTCTCC GTGGGAACTC CCAGAACTCT 
CCAGAAAGTT CAGCACAAGT AGGCTGTGGT GGTTCTGAAT CATTCCATAG GATCTTAAAC 
TTTTTGAAAA ATCTCCAATC ACGTGTTCAA GACAAGGCAG ATCACAAAAG AACACGGCCC 
CAGTCTCCAA AGATCGCGTC TACCAAGAAA GCAGGCAAGT CAGAATGTCA TTCAAAATCT 
CAAAATGCAG CAGTGAGAAG GAATCTTCAA TTTGTCTCAG GAGGAAAGAA CCTCTCTTCA 
AGGATGGGTA GTTCTGGGAC ATCAAAAGCA CCTGGAAAAG AAAATGCCTG CCAACGGACT 
CTCAAAAAAA CAAATAAGGG TGTCCCTCGT CCCTGTGTCA CTGCAGAAAT GCAGGAATTG 
ATGAATCAAA AAGTATCTGA AAGAAAGAAA AGATGCCTTG AAGCCAAAAT ATATGTAAAG 
AAAGCCTTGG AGTCAAGAGA TAAGTTGCAG AATGTAAAAG AGAAAGAAAT TCCATTCAAG 
AAGTTGAATG CCAAAGTCTT ACAAATGTCA TCCAAGAATA TTGGTTCTGA GCAACAATCC 
AAAGCCCAGG AAGCCACGGA AAACAAAAGG GAGGCAGCTC TAGAGCATGA AAAGCAGATG 
TTGGGCACTG TGTTAAATAA TAAAGAGCTT CAAGACAGAA CTTCCCATGA TATTAAAACT 
CCAGTTACAG TTGGGGCTTC TAAAAGTGGG GTTAACCCTG CTCTGCCGGT CTTCAAAAGT 
ATATCCCCTA GACCCTTGAA ACAGCAAGGC TTGGCCCCTC CTAGATCTTT ACCTCCAGCT 
TTGACTCTTC AGTCACATCA TCTTGAAAAC TTGAGGAAGA CTCAGAGCTC TATTGTACCT 
AACAGAGAAT CAAACTCTGC ACCCTACCAA CACAATCAGG AGAGGATCCG GATTCTCGAG 
GCCACTGCTC AGATGCTGAA AGAGAGAATT GAATTTCTGA CTGAGAAGTT AAACCTCTCA 
GGAGTATGTC TGACACTTAA TGGCAAATCT GAGAAGTGCA TGGATGTACA AAGCACCAAT 
ATCTCATCAG TTCAGGAGTG CCCCACAATG ATAGTATCAT CTTGTTCTCT TGTACTTGGG 
CAGTCTTCCA GCCCAACTGC CTTGGGAACC CCTGGAGATT TTACTCTGAC TGAGGATTCT 
GAAATGGAAG AGTGGGCACC AGAAGATAAA TTCACCTGTA CTCCTACTTT CTTCACAAGA 
AGTTCACAGA AAGAATTTGA AAAGCAAGAT AGCTTCATTG ATCCTTCTGG AATTCAGACG 
GAAGAAGAAC TGTCTGAATT TTCTCTTGGA ATCTCTGAAT CCTATAATGA CATCAAGATA 
CGAGACTTTC CCACCAGAAG AAATGAGTTT TCAAGCTCTT CAAGCAAACA TAGGGAGGAA 
TATGAAAGTT GCAACAAATA TTCCAAAGAT GTCTGCATGG ATTATTTAAG CAGCATGCAA 
CAGAAATCTC TTAACTTTCT TCAGAAAATG AAGTTACATC AGATAAAACA GGAAAGAGAG 
TTAGAAATTC TGAAGCATAG AGCAGAACTG GAAGCTGCAG AAACTCAGAA ATCTTTGGAT 
GAATTGGTCT TTAGGAATCA TTTGAAGCAG TCTTTGCACG GCAGCCACAT CCATAGAACT 
GAACTAGACA ATAAAGCAGA GAAATGGAAG ATGCCAAAGA CCCATGGAGG CCTGGAATCC 
ACAGACTGTC TAACTGGGCT GTCATCTATG GGCAGGTCAT ATTCCAGCAG AACCAGTAGT 
CCAACAGTTT CCACCTGGGA AAGTGAGCCA AGGAGAAATT TATTGGGTTG CACAGATGCT 
GATAAGGAAA GAGGGAAGAA GCAAGACTAT ACCACATCAT CACACTCCAC TACATGGTTC 
TCATCAGCTT TTGGTTATCC AAGTAGCAAG GTATTCTCTA AAGAAGACTC CATGGACAGC 
AGCAGCCAGA GGAATGAAAT CTATCGGTCT AGCCTCTTCG ACCGCTTTAG CCTGAGGATG 
ACTGATCAGT ATTTAAAGGA AGAAGAGCTG AGAGCACAGT ACCAGATAGC CCTCCTCAAG 
CTCCGGGAGA AAGCCCTAGA GGAGAAAGTT ATAGCTGAAT TGTCATGGCT AGAGCAACAG 
AAAGCGTGTT TGGGGAACAA AAGAGATCAC AGCTTAATGA CTGAGGAACA GCACCAAATA 
CTAATAGATC TGAAGAAAGA ACAGTTTTCT AGTCCCTGGT CAGAAGCTAT AGAAAGCCAG 
AAGTCTGAAG ATGAGAGAAT CTTCAGATTT GAGAGGTTGG TTAGCAGTCC AGAGGGTTTG 
TTGTCTTCAA CATCATCTCA TACAGAAAGA TCCTCTTCAG TCTTGCCTGA CCTTGGGGAA 
AACCTGAAAT GCTATGAGAA CACACACCTT CTTGCTGAGA GGGAGATCCA GGGGAAACCT 
GATGAGGACT CATGCCTACA GCCACAGTGG CTGAAGGGGG GAAAGGACAG ATCAATGGTC 
AGTAACATAC CTGACCACAG GGAGCAAACT CTGGGCCAGT TCGTGAAGAA TGACAACCAG 
GAAAATAAAG AACCTGCTGA ACAGCACTGT ACTAGCTGGG CATGTCGACA GCAAGAAAGC 
TCTTTGGAAA TCAAACAAGA TCCGTATCAG GTCTTTGCTA AGCCAACTAC AAGGACAGAG 
GAATATAATT CAAAGACTGA CTTCAAGCCT AATATGGTGA AAAAAAGACC TCAAAGTGAA 
GTAATAGGCT CATTAGAAAA TGGTGAGAGA TACATGCAAG GACAAAAAAA TGAACTAAAT 
TTTTTAAAAT TGGAAAGAAG TTTGGCTGGA CAAGAAGGTG GTCAATCTTT TCAGCAGGAA 
GACAGTGTTA TTTACAGTAG AAAAAATCAG GAGACCTCAG GACTGGACTC AGATGTCATT 
AAGAGTCCAA TAGTTGAACC AGAAGAGGAC AAAAGAAGGA AACAGAGGAT GAAGGGTAAA 
TCTCAAGAAG TTCCATTGAA AACTATCCAG ATATGTCACA CTTCTGCCAT TCAAGAATGC 
AAAAAAGATC AAGAGAAGAA ATCAGAGTAT ACTCAATATG ATGAAAACCA CTTGCATATT 
AAGTGTGATA CGACTTCTGA CTCCTTAAAA TCAGGATCTG TTTTCAGTAC TTCTTTAGAA 
TCTCTTTCTT CAGACACAAT ATCACCATGC AACTCACCTC TAGATTTAAA GAGTGATCAG 
TCATATACAT CTTTTTCTGA ATTTCAGAAA GTGACTGCCT TTCTAGTTAA TGTTTCAGAC 
AGTTTGATTT CAGGAAGTGA CTCAGAAACT GAGAGTTCAC AGAACACAGA TGTCAATGAA 
TGGCAGGAGT TCACTGACCA ACAACCATTG AATGAGCTTT CTAGTCCTTT GCCTAATCCA 
TCAGTAACTC CAAGGAAAGG AGATGAATTG ATTTTGGCAG TTAATAGTGA AGAGAAACAG 
CCAGATAGAG ATTTCCCTTT TGAAAACTGC CAAGACTTAC AGACACAAGG GAATAATAAT 
ATATCATCTA ATGACTCTCC TTCTGAAACA AATAATGTAA GTTTGTCAGG CAGCAGGAAA 
CACAAGCTAA GCCTTTCGTG GACAGAGTTA CCATTGTCTC AAGATTCATC CTCTGGAAAG 
CTGAGTCATA TTCTTTCAGA GGATACCTAT GTGGCACCAA GACAGGATAA AGAAAAGAGT 
TGCTCGGAGC ATAAAGTTTG TGTTGAACCC AAGCTCAGTT CCTTTTCATT CTTGGATGAC 
TTTTCAAAGA CAGATATAGA TTTGAAGCAG CAAGGTCCTA CAATTTCCCT GAATGAAGGA 
AAAGATCAGG AAGACCTTGA AATCAAAGAT GTTTTAGCGG ATGGAACAAA CAAAAAAGAA 
ACTGGATTTT CTCTCCCAGA AATTTATTAC ATGTCATTTC CATCCCCAAA TCTGAAAGCA 
AATTGCCAAT CACCTTCACT GCCTCTTAAA ATGTCACAGA GAAAACAAAA CTCGTTAGTC 
AAAAAAGCTA AAGCTGCATG TGAAGAAAAT CCAGAAGAAA GGCTTATTTC TAATAAAGAG 
TGTAAATATG CCATTCAGCA GTTGTCAATT GATTTCAACA GTATGCCTGC TATACCTGGC 
CCCCAGATCT TTCATGATGG AAAAATTGTT CAGTCAGTAT ATGAGCACAA GGCAGATAAA 
TCTGATATTC ATCACAGGAC CTCAGAGGGC CACGAGGTGG TGATATCTCC TTCTGGATCA 
CTTAGTGGGC TGATTCCTGA GGCTGAGCAT TGCCCAGCTA TCAGGGTTGA TGGTTCCACA 
CATACTGAAA GAGATGGTCT ACCTAGTGTC CAAGATAGCA TCTTGTCTAA AATACTATCC 
CCTGTCGACG AAGTGCTTTC TTATGGAAGT GCTGGCCTTC CATCCCCAAC CCAGAAGGTT 
TCATCTTTCG CCAGTGAAAA TCTCCCTTCT CCTCCTCCTG ACTTTGTGCT TTGGGCAAAT 
GTCAGTGATA TTGACTTCAA TTCAGAAGAC TTCCCTTCTC TGCCAGAAGA AATGGTATTT 
CCAGAAGATT CCAAGGATGT ACAAGATGAA GATTCATCTA TTCAAACTGG AGAGTTATCT 
TCTTTATCTG ATGAGATACT GCTGGAGGAA TTTTCTCCAA TACCTTTAGA TGTAGGGAAT 
TCCTGCTCAG TTCTGGATGA ACACTCCCTA GGATGGGACA AAGAGAGAGA AAGAAGTTTG 
GAAAGGAAGG AGGAATTGTT GAGAGTCGAA CCACAGCAGG ATATCAAAAG GAAGCAAAAA 
GTGGGCTGCT GGTACAGTCC TGCTTTGTCC AGAAGTGCCA GTAAAAGTCC AGCATCATTC 
TTCAAACTAT CCACAGATAA AGATGGCAAG AATCCACTGG CATTGGAGGA TGCAGAGAAC 
AGAGAACATA CACAACCAGA AACTCTGAAA TTCAGAGAGA CAAGCCATTT TGAAGGAAAA 
CACTGGACTA GTTTGGTATC CGATGAAGCA AAGAATGATC ATCACAAAGA AGACTTGGAA 
AGAGTAAAAC ATTCAGAGTA TGACATGGCT TATGATGAGT TATTTAGATC AGATAAAATG 
TCATACTTTT TGGCAGACGA CATAGAAAAG CCAAGTGACC ATAATTACCA AGATTCAGTT 
CATGATGAAT CATCCCTAAG GAGTAACAAG TGCTATGATG AGGTCAGAGT GGAATACCGG 
TTTTTAGAGA GAAAGCCAGA AATAAAGTTT AACAATAGAG ATAGCTCAAT GACAACAGTA 
GCCGCTTATC CTGAAACCCT GATGTTCCAA CCTACAGAGA TGGAGTCCTA CTTTATTCCT 
ACTCAGTCTG AGTCTGAAAG ATTTTTAAAT AAAGATTTCT CAGGCTGCCT AAATAGCCAA 
AAAGAACAGC CATATATGGC ACCTATAAAA AATGAGCCTG AGAAGTTACT TGAAGATGTT 
CATCCAATGG AAGAGAAAGA AATCAACTTA AAAAATAGTG TAATGGGCTC CCAACTTGAT 
CCTAGGCAAA AAACATATGG AATTGTAGAT TCTGTATCCA CTGAACTGAC CAGAAAAATC 
CTGTGGGATG CCTTAGCTGT GTTTGCAGAA CTTGCCAGAC AATAGAGTGT CTTCCTGTCC 
ATCAACTGCT GTTCAAAATT GCTAACAATG CCCTCTCTAA TAACTGCTTT GACAAACCAC 
ATATGTGTCA GTGACACAGA AAAGAGAAAA TGGAATTTTG ACAATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012409470.1
CDS	1..7011
Translation	MVFDYMTSNVPLSSLSSSSPSEQIQSRQKNSVYLSPQHLHIISPDVALAWVSLQRARHVLQRMESEMDTPSWHSISSQSCGSLDFGQEKLFSWENWHNASPAYAKLRMPHFSEDYNMAAASVSPWVFRESVIFREEPDGKTYGGRVTHLNPVIFGPVWKEQDFNYPPEPERMCTTSDWPSKMPVFIPVQSGSHHQFLNHPSDPWREKSPQPVESPTNFQRWPLTGVKRAFPESQLEQLREKIRAQKHRHANVFPTSFQARSGTPQTLPWKHVPKRSVSKMKLAASKPAHPGQVSASSPDPSDTHHAVSHKFIPKRKSYRVRFALSTPIDAHQRDKNKTTVTKSCQVSSQSLSLGKQLKGQDAKVTGVSAWRKGQRLARLLLGPPPTFLQLQKRALSGGLAATKESGLTEKTQKSEHIPPSTRDINNQKGAAPLRGNSQNSPESSAQVGCGGSESFHRILNFLKNLQSRVQDKADHKRTRPQSPKIASTKKAGKSECHSKSQNAAVRRNLQFVSGGKNLSSRMGSSGTSKAPGKENACQRTLKKTNKGVPRPCVTAEMQELMNQKVSERKKRCLEAKIYVKKALESRDKLQNVKEKEIPFKKLNAKVLQMSSKNIGSEQQSKAQEATENKREAALEHEKQMLGTVLNNKELQDRTSHDIKTPVTVGASKSGVNPALPVFKSISPRPLKQQGLAPPRSLPPALTLQSHHLENLRKTQSSIVPNRESNSAPYQHNQERIRILEATAQMLKERIEFLTEKLNLSGVCLTLNGKSEKCMDVQSTNISSVQECPTMIVSSCSLVLGQSSSPTALGTPGDFTLTEDSEMEEWAPEDKFTCTPTFFTRSSQKEFEKQDSFIDPSGIQTEEELSEFSLGISESYNDIKIRDFPTRRNEFSSSSSKHREEYESCNKYSKDVCMDYLSSMQQKSLNFLQKMKLHQIKQERELEILKHRAELEAAETQKSLDELVFRNHLKQSLHGSHIHRTELDNKAEKWKMPKTHGGLESTDCLTGLSSMGRSYSSRTSSPTVSTWESEPRRNLLGCTDADKERGKKQDYTTSSHSTTWFSSAFGYPSSKVFSKEDSMDSSSQRNEIYRSSLFDRFSLRMTDQYLKEEELRAQYQIALLKLREKALEEKVIAELSWLEQQKACLGNKRDHSLMTEEQHQILIDLKKEQFSSPWSEAIESQKSEDERIFRFERLVSSPEGLLSSTSSHTERSSSVLPDLGENLKCYENTHLLAEREIQGKPDEDSCLQPQWLKGGKDRSMVSNIPDHREQTLGQFVKNDNQENKEPAEQHCTSWACRQQESSLEIKQDPYQVFAKPTTRTEEYNSKTDFKPNMVKKRPQSEVIGSLENGERYMQGQKNELNFLKLERSLAGQEGGQSFQQEDSVIYSRKNQETSGLDSDVIKSPIVEPEEDKRRKQRMKGKSQEVPLKTIQICHTSAIQECKKDQEKKSEYTQYDENHLHIKCDTTSDSLKSGSVFSTSLESLSSDTISPCNSPLDLKSDQSYTSFSEFQKVTAFLVNVSDSLISGSDSETESSQNTDVNEWQEFTDQQPLNELSSPLPNPSVTPRKGDELILAVNSEEKQPDRDFPFENCQDLQTQGNNNISSNDSPSETNNVSLSGSRKHKLSLSWTELPLSQDSSSGKLSHILSEDTYVAPRQDKEKSCSEHKVCVEPKLSSFSFLDDFSKTDIDLKQQGPTISLNEGKDQEDLEIKDVLADGTNKKETGFSLPEIYYMSFPSPNLKANCQSPSLPLKMSQRKQNSLVKKAKAACEENPEERLISNKECKYAIQQLSIDFNSMPAIPGPQIFHDGKIVQSVYEHKADKSDIHHRTSEGHEVVISPSGSLSGLIPEAEHCPAIRVDGSTHTERDGLPSVQDSILSKILSPVDEVLSYGSAGLPSPTQKVSSFASENLPSPPPDFVLWANVSDIDFNSEDFPSLPEEMVFPEDSKDVQDEDSSIQTGELSSLSDEILLEEFSPIPLDVGNSCSVLDEHSLGWDKERERSLERKEELLRVEPQQDIKRKQKVGCWYSPALSRSASKSPASFFKLSTDKDGKNPLALEDAENREHTQPETLKFRETSHFEGKHWTSLVSDEAKNDHHKEDLERVKHSEYDMAYDELFRSDKMSYFLADDIEKPSDHNYQDSVHDESSLRSNKCYDEVRVEYRFLERKPEIKFNNRDSSMTTVAAYPETLMFQPTEMESYFIPTQSESERFLNKDFSGCLNSQKEQPYMAPIKNEPEKLLEDVHPMEEKEINLKNSVMGSQLDPRQKTYGIVDSVSTELTRKILWDALAVFAELARQ*SVFLSINCCSKLLTMPSLITALTNHICVSDTEKRKWNFDNE

Target ORF information:

RefSeq Version	XM_012554016.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii centrosome-associated protein 350-like (LOC100932056), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012554016.1

ORF Insert Sequence:

ATGGTATTTG ACTACATGAC CTCCAATGTT CCTCTCAGTT CTTTGAGCTC CTCCTCTCCA 
TCTGAACAGA TCCAATCAAG GCAGAAAAAC TCTGTCTACC TGAGCCCTCA GCATCTGCAT 
ATCATTTCCC CAGATGTGGC CTTGGCTTGG GTCAGCCTTC AAAGAGCCAG GCACGTGTTA 
CAGCGGATGG AGAGTGAAAT GGACACCCCC AGCTGGCACA GCATCAGCAG CCAGAGCTGC 
GGATCACTTG ATTTTGGGCA AGAGAAGCTG TTTTCTTGGG AAAACTGGCA CAATGCTAGT 
CCTGCCTATG CCAAACTGAG AATGCCTCAT TTTTCTGAGG ACTACAACAT GGCAGCTGCA 
TCAGTGTCAC CCTGGGTCTT TCGGGAGTCA GTTATCTTCA GAGAAGAACC AGATGGCAAG 
ACTTACGGTG GACGAGTAAC TCATTTGAAT CCTGTGATTT TTGGCCCAGT GTGGAAAGAG 
CAGGACTTTA ACTACCCACC TGAACCAGAG AGAATGTGTA CAACTAGTGA CTGGCCCTCC 
AAAATGCCCG TCTTTATTCC CGTTCAGTCG GGAAGCCATC ATCAGTTCTT GAATCACCCA 
AGTGACCCCT GGAGGGAAAA GTCACCTCAG CCAGTTGAGT CTCCCACTAA TTTTCAGAGA 
TGGCCATTGA CAGGGGTAAA GAGAGCTTTC CCTGAATCCC AGCTGGAGCA GCTGAGGGAG 
AAGATCCGGG CACAGAAACA TCGACATGCT AATGTTTTCC CTACCTCCTT CCAAGCTCGC 
TCTGGGACCC CCCAAACTCT GCCCTGGAAG CACGTGCCAA AGAGAAGCGT CTCCAAAATG 
AAGTTAGCTG CTTCAAAGCC AGCCCACCCG GGCCAAGTCT CGGCTTCCAG CCCAGATCCC 
TCTGACACTC ACCATGCCGT TTCCCACAAA TTTATACCAA AGAGAAAGAG CTACAGAGTT 
AGATTTGCAT TATCAACGCC AATAGACGCA CATCAAAGGG ATAAAAATAA AACTACAGTG 
ACCAAAAGCT GCCAGGTATC TTCTCAGTCA CTTTCTCTTG GAAAACAGCT AAAAGGCCAA 
GATGCTAAGG TGACTGGCGT TTCTGCATGG AGAAAGGGAC AGAGGCTTGC GAGGCTCCTC 
CTTGGGCCCC CTCCCACGTT TCTCCAGCTC CAGAAAAGGG CTCTCTCCGG AGGCCTGGCC 
GCCACAAAGG AGTCAGGACT GACAGAGAAG ACTCAAAAAT CTGAGCACAT ACCACCCTCC 
ACCCGAGATA TAAACAACCA GAAAGGAGCA GCTCCTCTCC GTGGGAACTC CCAGAACTCT 
CCAGAAAGTT CAGCACAAGT AGGCTGTGGT GGTTCTGAAT CATTCCATAG GATCTTAAAC 
TTTTTGAAAA ATCTCCAATC ACGTGTTCAA GACAAGGCAG ATCACAAAAG AACACGGCCC 
CAGTCTCCAA AGATCGCGTC TACCAAGAAA GCAGGCAAGT CAGAATGTCA TTCAAAATCT 
CAAAATGCAG CAGTGAGAAG GAATCTTCAA TTTGTCTCAG GAGGAAAGAA CCTCTCTTCA 
AGGATGGGTA GTTCTGGGAC ATCAAAAGCA CCTGGAAAAG AAAATGCCTG CCAACGGACT 
CTCAAAAAAA CAAATAAGGG TGTCCCTCGT CCCTGTGTCA CTGCAGAAAT GCAGGAATTG 
ATGAATCAAA AAGTATCTGA AAGAAAGAAA AGATGCCTTG AAGCCAAAAT ATATGTAAAG 
AAAGCCTTGG AGTCAAGAGA TAAGTTGCAG AATGTAAAAG AGAAAGAAAT TCCATTCAAG 
AAGTTGAATG CCAAAGTCTT ACAAATGTCA TCCAAGAATA TTGGTTCTGA GCAACAATCC 
AAAGCCCAGG AAGCCACGGA AAACAAAAGG GAGGCAGCTC TAGAGCATGA AAAGCAGATG 
TTGGGCACTG TGTTAAATAA TAAAGAGCTT CAAGACAGAA CTTCCCATGA TATTAAAACT 
CCAGTTACAG TTGGGGCTTC TAAAAGTGGG GTTAACCCTG CTCTGCCGGT CTTCAAAAGT 
ATATCCCCTA GACCCTTGAA ACAGCAAGGC TTGGCCCCTC CTAGATCTTT ACCTCCAGCT 
TTGACTCTTC AGTCACATCA TCTTGAAAAC TTGAGGAAGA CTCAGAGCTC TATTGTACCT 
AACAGAGAAT CAAACTCTGC ACCCTACCAA CACAATCAGG AGAGGATCCG GATTCTCGAG 
GCCACTGCTC AGATGCTGAA AGAGAGAATT GAATTTCTGA CTGAGAAGTT AAACCTCTCA 
GGAGTATGTC TGACACTTAA TGGCAAATCT GAGAAGTGCA TGGATGTACA AAGCACCAAT 
ATCTCATCAG TTCAGGAGTG CCCCACAATG ATAGTATCAT CTTGTTCTCT TGTACTTGGG 
CAGTCTTCCA GCCCAACTGC CTTGGGAACC CCTGGAGATT TTACTCTGAC TGAGGATTCT 
GAAATGGAAG AGTGGGCACC AGAAGATAAA TTCACCTGTA CTCCTACTTT CTTCACAAGA 
AGTTCACAGA AAGAATTTGA AAAGCAAGAT AGCTTCATTG ATCCTTCTGG AATTCAGACG 
GAAGAAGAAC TGTCTGAATT TTCTCTTGGA ATCTCTGAAT CCTATAATGA CATCAAGATA 
CGAGACTTTC CCACCAGAAG AAATGAGTTT TCAAGCTCTT CAAGCAAACA TAGGGAGGAA 
TATGAAAGTT GCAACAAATA TTCCAAAGAT GTCTGCATGG ATTATTTAAG CAGCATGCAA 
CAGAAATCTC TTAACTTTCT TCAGAAAATG AAGTTACATC AGATAAAACA GGAAAGAGAG 
TTAGAAATTC TGAAGCATAG AGCAGAACTG GAAGCTGCAG AAACTCAGAA ATCTTTGGAT 
GAATTGGTCT TTAGGAATCA TTTGAAGCAG TCTTTGCACG GCAGCCACAT CCATAGAACT 
GAACTAGACA ATAAAGCAGA GAAATGGAAG ATGCCAAAGA CCCATGGAGG CCTGGAATCC 
ACAGACTGTC TAACTGGGCT GTCATCTATG GGCAGGTCAT ATTCCAGCAG AACCAGTAGT 
CCAACAGTTT CCACCTGGGA AAGTGAGCCA AGGAGAAATT TATTGGGTTG CACAGATGCT 
GATAAGGAAA GAGGGAAGAA GCAAGACTAT ACCACATCAT CACACTCCAC TACATGGTTC 
TCATCAGCTT TTGGTTATCC AAGTAGCAAG GTATTCTCTA AAGAAGACTC CATGGACAGC 
AGCAGCCAGA GGAATGAAAT CTATCGGTCT AGCCTCTTCG ACCGCTTTAG CCTGAGGATG 
ACTGATCAGT ATTTAAAGGA AGAAGAGCTG AGAGCACAGT ACCAGATAGC CCTCCTCAAG 
CTCCGGGAGA AAGCCCTAGA GGAGAAAGTT ATAGCTGAAT TGTCATGGCT AGAGCAACAG 
AAAGCGTGTT TGGGGAACAA AAGAGATCAC AGCTTAATGA CTGAGGAACA GCACCAAATA 
CTAATAGATC TGAAGAAAGA ACAGTTTTCT AGTCCCTGGT CAGAAGCTAT AGAAAGCCAG 
AAGTCTGAAG ATGAGAGAAT CTTCAGATTT GAGAGGTTGG TTAGCAGTCC AGAGGGTTTG 
TTGTCTTCAA CATCATCTCA TACAGAAAGA TCCTCTTCAG TCTTGCCTGA CCTTGGGGAA 
AACCTGAAAT GCTATGAGAA CACACACCTT CTTGCTGAGA GGGAGATCCA GGGGAAACCT 
GATGAGGACT CATGCCTACA GCCACAGTGG CTGAAGGGGG GAAAGGACAG ATCAATGGTC 
AGTAACATAC CTGACCACAG GGAGCAAACT CTGGGCCAGT TCGTGAAGAA TGACAACCAG 
GAAAATAAAG AACCTGCTGA ACAGCACTGT ACTAGCTGGG CATGTCGACA GCAAGAAAGC 
TCTTTGGAAA TCAAACAAGA TCCGTATCAG GTCTTTGCTA AGCCAACTAC AAGGACAGAG 
GAATATAATT CAAAGACTGA CTTCAAGCCT AATATGGTGA AAAAAAGACC TCAAAGTGAA 
GTAATAGGCT CATTAGAAAA TGGTGAGAGA TACATGCAAG GACAAAAAAA TGAACTAAAT 
TTTTTAAAAT TGGAAAGAAG TTTGGCTGGA CAAGAAGGTG GTCAATCTTT TCAGCAGGAA 
GACAGTGTTA TTTACAGTAG AAAAAATCAG GAGACCTCAG GACTGGACTC AGATGTCATT 
AAGAGTCCAA TAGTTGAACC AGAAGAGGAC AAAAGAAGGA AACAGAGGAT GAAGGGTAAA 
TCTCAAGAAG TTCCATTGAA AACTATCCAG ATATGTCACA CTTCTGCCAT TCAAGAATGC 
AAAAAAGATC AAGAGAAGAA ATCAGAGTAT ACTCAATATG ATGAAAACCA CTTGCATATT 
AAGTGTGATA CGACTTCTGA CTCCTTAAAA TCAGGATCTG TTTTCAGTAC TTCTTTAGAA 
TCTCTTTCTT CAGACACAAT ATCACCATGC AACTCACCTC TAGATTTAAA GAGTGATCAG 
TCATATACAT CTTTTTCTGA ATTTCAGAAA GTGACTGCCT TTCTAGTTAA TGTTTCAGAC 
AGTTTGATTT CAGGAAGTGA CTCAGAAACT GAGAGTTCAC AGAACACAGA TGTCAATGAA 
TGGCAGGAGT TCACTGACCA ACAACCATTG AATGAGCTTT CTAGTCCTTT GCCTAATCCA 
TCAGTAACTC CAAGGAAAGG AGATGAATTG ATTTTGGCAG TTAATAGTGA AGAGAAACAG 
CCAGATAGAG ATTTCCCTTT TGAAAACTGC CAAGACTTAC AGACACAAGG GAATAATAAT 
ATATCATCTA ATGACTCTCC TTCTGAAACA AATAATGTAA GTTTGTCAGG CAGCAGGAAA 
CACAAGCTAA GCCTTTCGTG GACAGAGTTA CCATTGTCTC AAGATTCATC CTCTGGAAAG 
CTGAGTCATA TTCTTTCAGA GGATACCTAT GTGGCACCAA GACAGGATAA AGAAAAGAGT 
TGCTCGGAGC ATAAAGTTTG TGTTGAACCC AAGCTCAGTT CCTTTTCATT CTTGGATGAC 
TTTTCAAAGA CAGATATAGA TTTGAAGCAG CAAGGTCCTA CAATTTCCCT GAATGAAGGA 
AAAGATCAGG AAGACCTTGA AATCAAAGAT GTTTTAGCGG ATGGAACAAA CAAAAAAGAA 
ACTGGATTTT CTCTCCCAGA AATTTATTAC ATGTCATTTC CATCCCCAAA TCTGAAAGCA 
AATTGCCAAT CACCTTCACT GCCTCTTAAA ATGTCACAGA GAAAACAAAA CTCGTTAGTC 
AAAAAAGCTA AAGCTGCATG TGAAGAAAAT CCAGAAGAAA GGCTTATTTC TAATAAAGAG 
TGTAAATATG CCATTCAGCA GTTGTCAATT GATTTCAACA GTATGCCTGC TATACCTGGC 
CCCCAGATCT TTCATGATGG AAAAATTGTT CAGTCAGTAT ATGAGCACAA GGCAGATAAA 
TCTGATATTC ATCACAGGAC CTCAGAGGGC CACGAGGTGG TGATATCTCC TTCTGGATCA 
CTTAGTGGGC TGATTCCTGA GGCTGAGCAT TGCCCAGCTA TCAGGGTTGA TGGTTCCACA 
CATACTGAAA GAGATGGTCT ACCTAGTGTC CAAGATAGCA TCTTGTCTAA AATACTATCC 
CCTGTCGACG AAGTGCTTTC TTATGGAAGT GCTGGCCTTC CATCCCCAAC CCAGAAGGTT 
TCATCTTTCG CCAGTGAAAA TCTCCCTTCT CCTCCTCCTG ACTTTGTGCT TTGGGCAAAT 
GTCAGTGATA TTGACTTCAA TTCAGAAGAC TTCCCTTCTC TGCCAGAAGA AATGGTATTT 
CCAGAAGATT CCAAGGATGT ACAAGATGAA GATTCATCTA TTCAAACTGG AGAGTTATCT 
TCTTTATCTG ATGAGATACT GCTGGAGGAA TTTTCTCCAA TACCTTTAGA TGTAGGGAAT 
TCCTGCTCAG TTCTGGATGA ACACTCCCTA GGATGGGACA AAGAGAGAGA AAGAAGTTTG 
GAAAGGAAGG AGGAATTGTT GAGAGTCGAA CCACAGCAGG ATATCAAAAG GAAGCAAAAA 
GTGGGCTGCT GGTACAGTCC TGCTTTGTCC AGAAGTGCCA GTAAAAGTCC AGCATCATTC 
TTCAAACTAT CCACAGATAA AGATGGCAAG AATCCACTGG CATTGGAGGA TGCAGAGAAC 
AGAGAACATA CACAACCAGA AACTCTGAAA TTCAGAGAGA CAAGCCATTT TGAAGGAAAA 
CACTGGACTA GTTTGGTATC CGATGAAGCA AAGAATGATC ATCACAAAGA AGACTTGGAA 
AGAGTAAAAC ATTCAGAGTA TGACATGGCT TATGATGAGT TATTTAGATC AGATAAAATG 
TCATACTTTT TGGCAGACGA CATAGAAAAG CCAAGTGACC ATAATTACCA AGATTCAGTT 
CATGATGAAT CATCCCTAAG GAGTAACAAG TGCTATGATG AGGTCAGAGT GGAATACCGG 
TTTTTAGAGA GAAAGCCAGA AATAAAGTTT AACAATAGAG ATAGCTCAAT GACAACAGTA 
GCCGCTTATC CTGAAACCCT GATGTTCCAA CCTACAGAGA TGGAGTCCTA CTTTATTCCT 
ACTCAGTCTG AGTCTGAAAG ATTTTTAAAT AAAGATTTCT CAGGCTGCCT AAATAGCCAA 
AAAGAACAGC CATATATGGC ACCTATAAAA AATGAGCCTG AGAAGTTACT TGAAGATGTT 
CATCCAATGG AAGAGAAAGA AATCAACTTA AAAAATAGTG TAATGGGCTC CCAACTTGAT 
CCTAGGCAAA AAACATATGG AATTGTAGAT TCTGTATCCA CTGAACTGAC CAGAAAAATC 
CTGTGGGATG CCTTAGCTGT GTTTGCAGAA CTTGCCAGAC AATAGAGTGT CTTCCTGTCC 
ATCAACTGCT GTTCAAAATT GCTAACAATG CCCTCTCTAA TAACTGCTTT GACAAACCAC 
ATATGTGTCA GTGACACAGA AAAGAGAAAA TGGAATTTTG ACAATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100932056 ( XM_023494340.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023350108.1 Sarcophilus harrisii centrosome-associated protein 350-like (LOC100932056), mRNA.

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CloneID	OSa150401
Clone ID Related Accession (Same CDS sequence)	XM_023494340.1
Accession Version	XM_023494340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	LOW QUALITY PROTEIN: centrosome-associated protein 350-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816666.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGGAGAGTG AAATGGACAC CCCCAGCTGG CACAGCATCA GCAGCCAGAG CTGCGGATCA 
CTTGATTTTG GGCAAGAGAA GCTGTTTTCT TGGGAAAACT GGCACAATGC TAGTCCTGCC 
TATGCCAAAC TGAGAATGCC TCATTTTTCT GAGGACTACA ACATGGCAGC TGCATCAGTG 
TCACCCTGGG TCTTTCGGGA GTCAGTTATC TTCAGAGAAG AACCAGATGG CAAGACTTAC 
GGTGGACGAG TAACTCATTT GAATCCTGTG ATTTTTGGCC CAGTGTGGAA AGAGCAGGAC 
TTTAACTACC CACCTGAACC AGAGAGAATG TGTACAACTA GTGACTGGCC CTCCAAAATG 
CCCGTCTTTA TTCCCGTTCA GTCGGGAAGC CATCATCAGT TCTTGAATCA CCCAAGTGAC 
CCCTGGAGGG AAAAGTCACC TCAGCCAGTT GAGTCTCCCA CTAATTTTCA GAGATGGCCA 
TTGACAGGGG TAAAGAGAGC TTTCCCTGAA TCCCAGCTGG AGCAGCTGAG GGAGAAGATC 
CGGGCACAGA AACATCGACA TGCTAATGTT TTCCCTACCT CCTTCCAAGC TCGCTCTGGG 
ACCCCCCAAA CTCTGCCCTG GAAGCACGTG CCAAAGAGAA GCGTCTCCAA AATGAAGTTA 
GCTGCTTCAA AGCCAGCCCA CCCGGGCCAA GTCTCGGCTT CCAGCCCAGA TCCCTCTGAC 
ACTCACCATG CCGTTTCCCA CAAATTTATA CCAAAGAGAA AGAGCTACAG AGTTAGATTT 
GCATTATCAA CGCCAATAGA CGCACATCAA AGGGATAAAA ATAAAACTAC AGTGACCAAA 
AGCTGCCAGG TATCTTCTCA GTCACTTTCT CTTGGAAAAC AGCTAAAAGG CCAAGATGCT 
AAGGTGACTG GCGTTTCTGC ATGGAGAAAG GGACAGAGGC TTGCGAGGCT CCTCCTTGGG 
CCCCCTCCCA CGTTTCTCCA GCTCCAGAAA AGGGCTCTCT CCGGAGGCCT GGCCGCCACA 
AAGGAGTCAG GACTGACAGA GAAGACTCAA AAATCTGAGC ACATACCACC CTCCACCCGA 
GATATAAACA ACCAGAAAGG AGCAGCTCCT CTCCGTGGGA ACTCCCAGAA CTCTCCAGAA 
AGTTCAGCAC AAGTAGGCTG TGGTGGTTCT GAATCATTCC ATAGGATCTT AAACTTTTTG 
AAAAATCTCC AATCACGTGT TCAAGACAAG GCAGATCACA AAAGAACACG GCCCCAGTCT 
CCAAAGATCG CGTCTACCAA GAAAGCAGGC AAGTCAGAAT GTCATTCAAA ATCTCAAAAT 
GCAGCAGTGA GAAGGAATCT TCAATTTGTC TCAGGAGGAA AGAACCTCTC TTCAAGGATG 
GGTAGTTCTG GGACATCAAA AGCACCTGGA AAAGAAAATG CCTGCCAACG GACTCTCAAA 
AAAACAAATA AGGGTGTCCC TCGTCCCTGT GTCACTGCAG AAATGCAGGA ATTGATGAAT 
CAAAAAGTAT CTGAAAGAAA GAAAAGATGC CTTGAAGCCA AAATATATGT AAAGAAAGCC 
TTGGAGTCAA GAGATAAGTT GCAGAATGTA AAAGAGAAAG AAATTCCATT CAAGAAGTTG 
AATGCCAAAG TCTTACAAAT GTCATCCAAG AATATTGGTT CTGAGCAACA ATCCAAAGCC 
CAGGAAGCCA CGGAAAACAA AAGGGAGGCA GCTCTAGAGC ATGAAAAGCA GATGTTGGGC 
ACTGTGTTAA ATAATAAAGA GCTTCAAGAC AGAACTTCCC ATGATATTAA AACTCCAGTT 
ACAGTTGGGG CTTCTAAAAG TGGGGTTAAC CCTGCTCTGC CGGTCTTCAA AAGTATATCC 
CCTAGACCCT TGAAACAGCA AGGCTTGGCC CCTCCTAGAT CTTTACCTCC AGCTTTGACT 
CTTCAGTCAC ATCATCTTGA AAACTTGAGG AAGACTCAGA GCTCTATTGT ACCTAACAGA 
GAATCAAACT CTGCACCCTA CCAACACAAT CAGGAGAGGA TCCGGATTCT CGAGGCCACT 
GCTCAGATGC TGAAAGAGAG AATTGAATTT CTGACTGAGA AGTTAAACCT CTCAGGAGTA 
TGTCTGACAC TTAATGGCAA ATCTGAGAAG TGCATGGATG TACAAAGCAC CAATATCTCA 
TCAGTTCAGG AGTGCCCCAC AATGATAGTA TCATCTTGTT CTCTTGTACT TGGGCAGTCT 
TCCAGCCCAA CTGCCTTGGG AACCCCTGGA GGTAATGAAC CAGAAGTGAA CAAGCCCCTC 
AGTGACAGAG AGAAAGAGAT GACTCAGAAA GGTGGAAAAG ATGGAGTGGA AATGGAAACA 
AAGGTCCCAG CTGTTCTCAG CCCTGCTGCA ACTGCTGAAA TGTTATCCTG TAGAAGCCCA 
CGATTGAATA AGCAACCAAG TCCTTGCCTT GAGGAAGTAA TGAAACCTGT ACCTGATGAT 
TTTACTCTGA CTGAGGATTC TGAAATGGAA GAGTGGGCAC CAGAAGATAA ATTCACCTGT 
ACTCCTACTT TCTTCACAAG AAGTTCACAG AAAGAATTTG AAAAGCAAGA TAGCTTCATT 
GATCCTTCTG GAATTCAGAC GGAAGAAGAA CTGTCTGAAT TTTCTCTTGG AATCTCTGAA 
TCCTATAATG ACATCAAGAT ACGAGACTTT CCCACCAGAA GAAATGAGTT TTCAAGCTCT 
TCAAGCAAAC ATAGGGAGGA ATATGAAAGT TGCAACAAAT ATTCCAAAGA TGTCTGCATG 
GATTATTTAA GCAGCATGCA ACAGAAATCT CTTAACTTTC TTCAGAAAAT GAAGTTACAT 
CAGATAAAAC AGGAAAGAGA GTTAGAAATT CTGAAGCATA GAGCAGAACT GGAAGCTGCA 
GAAACTCAGA AATCTTTGGA TGAATTGGTC TTTAGGAATC ATTTGAAGCA GTCTTTGCAC 
GGCAGCCACA TCCATAGAAC TGAACTAGAC AATAAAGCAG AGAAATGGAA GATGCCAAAG 
ACCCATGGAG GCCTGGAATC CACAGACTGT CTAACTGGGC TGTCATCTAT GGGCAGGTCA 
TATTCCAGCA GAACCAGTAG TCCAACAGTT TCCACCTGGG AAAGTGAGCC AAGGAGAAAT 
TTATTGGGTT GCACAGATGC TGATAAGGAA AGAGGGAAGA AGCAAGACTA TACCACATCA 
TCACACTCCA CTACATGGTT CTCATCAGCT TTTGGTTATC CAAGTAGCAA GGTATTCTCT 
AAAGAAGACT CCATGGACAG CAGCAGCCAG AGGAATGAAA TCTATCGGTC TAGCCTCTTC 
GACCGCTTTA GCCTGAGGAT GACTGATCAG TATTTAAAGG AAGAAGAGCT GAGAGCACAG 
TACCAGATAG CCCTCCTCAA GCTCCGGGAG AAAGCCCTAG AGGAGAAAGT TATAGCTGAA 
TTGTCATGGC TAGAGCAACA GAAAGCGTGT TTGGGGAACA AAAGAGATCA CAGCTTAATG 
ACTGAGGAAC AGCACCAAAT ACTAATAGAT CTGAAGAAAG AACAGTTTTC TAGTCCCTGG 
TCAGAAGCTA TAGAAAGCCA GAAGTCTGAA GATGAGAGAA TCTTCAGATT TGAGAGGTTG 
GTTAGCAGTC CAGAGGGTTT GTTGTCTTCA ACATCATCTC ATACAGAAAG ATCCTCTTCA 
GTCTTGCCTG ACCTTGGGGA AAACCTGAAA TGCTATGAGA ACACACACCT TCTTGCTGAG 
AGGGAGATCC AGGGGAAACC TGATGAGGAC TCATGCCTAC AGCCACAGTG GCTGAAGGGG 
GGAAAGGACA GATCAATGGT CAGTAACATA CCTGACCACA GGGAGCAAAC TCTGGGCCAG 
TTCGTGAAGA ATGACAACCA GGAAAATAAA GAACCTGCTG AACAGCACTG TACTAGCTGG 
GCATGTCGAC AGCAAGAAAG CTCTTTGGAA ATCAAACAAG ATCCGTATCA GGTCTTTGCT 
AAGCCAACTA CAAGGACAGA GGAATATAAT TCAAAGACTG ACTTCAAGCC TAATATGGTG 
AAAAAAAGAC CTCAAAGTGA AGTAATAGGC TCATTAGAAA ATGGTGAGAG ATACATGCAA 
GGACAAAAAA ATGAACTAAA TTTTTTAAAA TTGGAAAGAA GTTTGGCTGG ACAAGAAGGT 
GGTCAATCTT TTCAGCAGGA AGACAGTGTT ATTTACAGTA GAAAAAATCA GGAGACCTCA 
GGACTGGACT CAGATGTCAT TAAGAGTCCA ATAGTTGAAC CAGAAGAGGA CAAAAGAAGG 
AAACAGAGGA TGAAGGGTAA ATCTCAAGAA GTTCCATTGA AAACTATCCA GATATGTCAC 
ACTTCTGCCA TTCAAGAATG CAAAAAAGAT CAAGAGAAGA AATCAGAGTA TACTCAATAT 
GATGAAAACC ACTTGCATAT TAAGTGTGAT ACGACTTCTG ACTCCTTAAA ATCAGGATCT 
GTTTTCAGTA CTTCTTTAGA ATCTCTTTCT TCAGACACAA TATCACCATG CAACTCACCT 
CTAGATTTAA AGAGTGATCA GTCATATACA TCTTTTTCTG AATTTCAGAA AGTGACTGCC 
TTTCTAGTTA ATGTTTCAGA CAGTTTGATT TCAGGAAGTG ACTCAGAAAC TGAGAGTTCA 
CAGAACACAG ATGTCAATGA ATGGCAGGAG TTCACTGACC AACAACCATT GAATGAGCTT 
TCTAGTCCTT TGCCTAATCC ATCAGTAACT CCAAGGAAAG GAGATGAATT GATTTTGGCA 
GTTAATAGTG AAGAGAAACA GCCAGATAGA GATTTCCCTT TTGAAAACTG CCAAGACTTA 
CAGACACAAG GGAATAATAA TATATCATCT AATGACTCTC CTTCTGAAAC AAATAATGTA 
AGTTTGTCAG GCAGCAGGAA ACACAAGCTA AGCCTTTCGT GGACAGAGTT ACCATTGTCT 
CAAGATTCAT CCTCTGGAAA GCTGAGTCAT ATTCTTTCAG AGGATACCTA TGTGGCACCA 
AGACAGGATA AAGAAAAGAG TTGCTCGGAG CATAAAGTTT GTGTTGAACC CAAGCTCAGT 
TCCTTTTCAT TCTTGGATGA CTTTTCAAAG ACAGATATAG ATTTGAAGCA GCAAGGTCCT 
ACAATTTCCC TGAATGAAGG AAAAGATCAG GAAGACCTTG AAATCAAAGA TGTTTTAGCG 
GATGGAACAA ACAAAAAAGA AACTGGATTT TCTCTCCCAG AAATTTATTA CATGTCATTT 
CCATCCCCAA ATCTGAAAGC AAATTGCCAA TCACCTTCAC TGCCTCTTAA AATGTCACAG 
AGAAAACAAA ACTCGTTAGT CAAAAAAGCT AAAGCTGCAT GTGAAGAAAA TCCAGAAGAA 
AGGCTTATTT CTAATAAAGA GTGTAAATAT GCCATTCAGC AGTTGTCAAT TGATTTCAAC 
AGTATGCCTG CTATACCTGG CCCCCAGATC TTTCATGATG GAAAAATTGT TCAGTCAGTA 
TATGAGCACA AGGCAGATAA ATCTGATATT CATCACAGGA CCTCAGAGGG CCACGAGGTG 
GTGATATCTC CTTCTGGATC ACTTAGTGGG CTGATTCCTG AGGCTGAGCA TTGCCCAGCT 
ATCAGGGTTG ATGGTTCCAC ACATACTGAA AGAGATGGTC TACCTAGTGT CCAAGATAGC 
ATCTTGTCTA AAATACTATC CCCTGTCGAC GAAGTGCTTT CTTATGGAAG TGCTGGCCTT 
CCATCCCCAA CCCAGAAGGT TTCATCTTTC GCCAGTGAAA ATCTCCCTTC TCCTCCTCCT 
GACTTTGTGC TTTGGGCAAA TGTCAGTGAT ATTGACTTCA ATTCAGAAGA CTTCCCTTCT 
CTGCCAGAAG AAATGGTATT TCCAGAAGAT TCCAAGGATG TACAAGATGA AGATTCATCT 
ATTCAAACTG GAGAGTTATC TTCTTTATCT GATGAGATAC TGCTGGAGGA ATTTTCTCCA 
ATACCTTTAG ATGTAGGGAA TTCCTGCTCA GTTCTGGATG AACACTCCCT AGGATGGGAC 
AAAGAGAGAG AAAGAAGTTT GGAAAGGAAG GAGGAATTGT TGAGAGTCGA ACCACAGCAG 
GATATCAAAA GGAAGCAAAA AGTGGGCTGC TGGTACAGTC CTGCTTTGTC CAGAAGTGCC 
AGTAAAAGTC CAGCATCATT CTTCAAACTA TCCACAGATA AAGATGGCAA GAATCCACTG 
GCATTGGAGG ATGCAGAGAA CAGAGAACAT ACACAACCAG AAACTCTGAA ATTCAGAGAG 
ACAAGCCATT TTGAAGGAAA ACACTGGACT AGTTTGGTAT CCGATGAAGC AAAGAATGAT 
CATCACAAAG AAGACTTGGA AAGAGTAAAA CATTCAGAGT ATGACATGGC TTATGATGAG 
TTATTTAGAT CAGATAAAAT GTCATACTTT TTGGCAGACG ACATAGAAAA GCCAAGTGAC 
CATAATTACC AAGATTCAGT TCATGATGAA TCATCCCTAA GGAGTAACAA GTGCTATGAT 
GAGGTCAGAG TGGAATACCG GTTTTTAGAG AGAAAGCCAG AAATAAAGTT TAACAATAGA 
GATAGCTCAA TGACAACAGT AGCCGCTTAT CCTGAAACCC TGATGTTCCA ACCTACAGAG 
ATGGAGTCCT ACTTTATTCC TACTCAGTCT GAGTCTGAAA GATTTTTAAA TAAAGATTTC 
TCAGGCTGCC TAAATAGCCA AAAAGAACAG CCATATATGG CACCTATAAA AAATGAGCCT 
GAGAAGTTAC TTGAAGATGT TCATCCAATG GAAGAGAAAG AAATCAACTT AAAAAATAGT 
GTAATGGGCT CCCAACTTGA TCCTAGGCAA AAAACATATG GAATTGTAGA TTCTGTATCC 
ACTGAACTGA CCAGAAAAAT CCTGTGGGAT GCCTTAGCTG TGTTTGCAGA ACTTGCCAGA 
CAATAGAGTG TCTTCCTGTC CATCAACTGC TGTTGTAACC CCCTGATGAG GGAAAGCTCT 
GGGGTTTTCT ATGAAAAAAC AAAATTGCTA ACAATGCCCT CTCTAATAAC TGCTTTGACA 
AACCACATAT GTGTCAGTGA CACAGAAAAG AGAAAATGGA ATTTTGACAA TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023350108.1
CDS	10..7086
Translation	MESEMDTPSWHSISSQSCGSLDFGQEKLFSWENWHNASPAYAKLRMPHFSEDYNMAAASVSPWVFRESVIFREEPDGKTYGGRVTHLNPVIFGPVWKEQDFNYPPEPERMCTTSDWPSKMPVFIPVQSGSHHQFLNHPSDPWREKSPQPVESPTNFQRWPLTGVKRAFPESQLEQLREKIRAQKHRHANVFPTSFQARSGTPQTLPWKHVPKRSVSKMKLAASKPAHPGQVSASSPDPSDTHHAVSHKFIPKRKSYRVRFALSTPIDAHQRDKNKTTVTKSCQVSSQSLSLGKQLKGQDAKVTGVSAWRKGQRLARLLLGPPPTFLQLQKRALSGGLAATKESGLTEKTQKSEHIPPSTRDINNQKGAAPLRGNSQNSPESSAQVGCGGSESFHRILNFLKNLQSRVQDKADHKRTRPQSPKIASTKKAGKSECHSKSQNAAVRRNLQFVSGGKNLSSRMGSSGTSKAPGKENACQRTLKKTNKGVPRPCVTAEMQELMNQKVSERKKRCLEAKIYVKKALESRDKLQNVKEKEIPFKKLNAKVLQMSSKNIGSEQQSKAQEATENKREAALEHEKQMLGTVLNNKELQDRTSHDIKTPVTVGASKSGVNPALPVFKSISPRPLKQQGLAPPRSLPPALTLQSHHLENLRKTQSSIVPNRESNSAPYQHNQERIRILEATAQMLKERIEFLTEKLNLSGVCLTLNGKSEKCMDVQSTNISSVQECPTMIVSSCSLVLGQSSSPTALGTPGGNEPEVNKPLSDREKEMTQKGGKDGVEMETKVPAVLSPAATAEMLSCRSPRLNKQPSPCLEEVMKPVPDDFTLTEDSEMEEWAPEDKFTCTPTFFTRSSQKEFEKQDSFIDPSGIQTEEELSEFSLGISESYNDIKIRDFPTRRNEFSSSSSKHREEYESCNKYSKDVCMDYLSSMQQKSLNFLQKMKLHQIKQERELEILKHRAELEAAETQKSLDELVFRNHLKQSLHGSHIHRTELDNKAEKWKMPKTHGGLESTDCLTGLSSMGRSYSSRTSSPTVSTWESEPRRNLLGCTDADKERGKKQDYTTSSHSTTWFSSAFGYPSSKVFSKEDSMDSSSQRNEIYRSSLFDRFSLRMTDQYLKEEELRAQYQIALLKLREKALEEKVIAELSWLEQQKACLGNKRDHSLMTEEQHQILIDLKKEQFSSPWSEAIESQKSEDERIFRFERLVSSPEGLLSSTSSHTERSSSVLPDLGENLKCYENTHLLAEREIQGKPDEDSCLQPQWLKGGKDRSMVSNIPDHREQTLGQFVKNDNQENKEPAEQHCTSWACRQQESSLEIKQDPYQVFAKPTTRTEEYNSKTDFKPNMVKKRPQSEVIGSLENGERYMQGQKNELNFLKLERSLAGQEGGQSFQQEDSVIYSRKNQETSGLDSDVIKSPIVEPEEDKRRKQRMKGKSQEVPLKTIQICHTSAIQECKKDQEKKSEYTQYDENHLHIKCDTTSDSLKSGSVFSTSLESLSSDTISPCNSPLDLKSDQSYTSFSEFQKVTAFLVNVSDSLISGSDSETESSQNTDVNEWQEFTDQQPLNELSSPLPNPSVTPRKGDELILAVNSEEKQPDRDFPFENCQDLQTQGNNNISSNDSPSETNNVSLSGSRKHKLSLSWTELPLSQDSSSGKLSHILSEDTYVAPRQDKEKSCSEHKVCVEPKLSSFSFLDDFSKTDIDLKQQGPTISLNEGKDQEDLEIKDVLADGTNKKETGFSLPEIYYMSFPSPNLKANCQSPSLPLKMSQRKQNSLVKKAKAACEENPEERLISNKECKYAIQQLSIDFNSMPAIPGPQIFHDGKIVQSVYEHKADKSDIHHRTSEGHEVVISPSGSLSGLIPEAEHCPAIRVDGSTHTERDGLPSVQDSILSKILSPVDEVLSYGSAGLPSPTQKVSSFASENLPSPPPDFVLWANVSDIDFNSEDFPSLPEEMVFPEDSKDVQDEDSSIQTGELSSLSDEILLEEFSPIPLDVGNSCSVLDEHSLGWDKERERSLERKEELLRVEPQQDIKRKQKVGCWYSPALSRSASKSPASFFKLSTDKDGKNPLALEDAENREHTQPETLKFRETSHFEGKHWTSLVSDEAKNDHHKEDLERVKHSEYDMAYDELFRSDKMSYFLADDIEKPSDHNYQDSVHDESSLRSNKCYDEVRVEYRFLERKPEIKFNNRDSSMTTVAAYPETLMFQPTEMESYFIPTQSESERFLNKDFSGCLNSQKEQPYMAPIKNEPEKLLEDVHPMEEKEINLKNSVMGSQLDPRQKTYGIVDSVSTELTRKILWDALAVFAELARQ*SVFLSINCCCNPLMRESSGVFYEKTKLLTMPSLITALTNHICVSDTEKRKWNFDNE

Target ORF information:

RefSeq Version	XM_023494340.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii centrosome-associated protein 350-like (LOC100932056), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023494340.1

ORF Insert Sequence:

ATGGAGAGTG AAATGGACAC CCCCAGCTGG CACAGCATCA GCAGCCAGAG CTGCGGATCA 
CTTGATTTTG GGCAAGAGAA GCTGTTTTCT TGGGAAAACT GGCACAATGC TAGTCCTGCC 
TATGCCAAAC TGAGAATGCC TCATTTTTCT GAGGACTACA ACATGGCAGC TGCATCAGTG 
TCACCCTGGG TCTTTCGGGA GTCAGTTATC TTCAGAGAAG AACCAGATGG CAAGACTTAC 
GGTGGACGAG TAACTCATTT GAATCCTGTG ATTTTTGGCC CAGTGTGGAA AGAGCAGGAC 
TTTAACTACC CACCTGAACC AGAGAGAATG TGTACAACTA GTGACTGGCC CTCCAAAATG 
CCCGTCTTTA TTCCCGTTCA GTCGGGAAGC CATCATCAGT TCTTGAATCA CCCAAGTGAC 
CCCTGGAGGG AAAAGTCACC TCAGCCAGTT GAGTCTCCCA CTAATTTTCA GAGATGGCCA 
TTGACAGGGG TAAAGAGAGC TTTCCCTGAA TCCCAGCTGG AGCAGCTGAG GGAGAAGATC 
CGGGCACAGA AACATCGACA TGCTAATGTT TTCCCTACCT CCTTCCAAGC TCGCTCTGGG 
ACCCCCCAAA CTCTGCCCTG GAAGCACGTG CCAAAGAGAA GCGTCTCCAA AATGAAGTTA 
GCTGCTTCAA AGCCAGCCCA CCCGGGCCAA GTCTCGGCTT CCAGCCCAGA TCCCTCTGAC 
ACTCACCATG CCGTTTCCCA CAAATTTATA CCAAAGAGAA AGAGCTACAG AGTTAGATTT 
GCATTATCAA CGCCAATAGA CGCACATCAA AGGGATAAAA ATAAAACTAC AGTGACCAAA 
AGCTGCCAGG TATCTTCTCA GTCACTTTCT CTTGGAAAAC AGCTAAAAGG CCAAGATGCT 
AAGGTGACTG GCGTTTCTGC ATGGAGAAAG GGACAGAGGC TTGCGAGGCT CCTCCTTGGG 
CCCCCTCCCA CGTTTCTCCA GCTCCAGAAA AGGGCTCTCT CCGGAGGCCT GGCCGCCACA 
AAGGAGTCAG GACTGACAGA GAAGACTCAA AAATCTGAGC ACATACCACC CTCCACCCGA 
GATATAAACA ACCAGAAAGG AGCAGCTCCT CTCCGTGGGA ACTCCCAGAA CTCTCCAGAA 
AGTTCAGCAC AAGTAGGCTG TGGTGGTTCT GAATCATTCC ATAGGATCTT AAACTTTTTG 
AAAAATCTCC AATCACGTGT TCAAGACAAG GCAGATCACA AAAGAACACG GCCCCAGTCT 
CCAAAGATCG CGTCTACCAA GAAAGCAGGC AAGTCAGAAT GTCATTCAAA ATCTCAAAAT 
GCAGCAGTGA GAAGGAATCT TCAATTTGTC TCAGGAGGAA AGAACCTCTC TTCAAGGATG 
GGTAGTTCTG GGACATCAAA AGCACCTGGA AAAGAAAATG CCTGCCAACG GACTCTCAAA 
AAAACAAATA AGGGTGTCCC TCGTCCCTGT GTCACTGCAG AAATGCAGGA ATTGATGAAT 
CAAAAAGTAT CTGAAAGAAA GAAAAGATGC CTTGAAGCCA AAATATATGT AAAGAAAGCC 
TTGGAGTCAA GAGATAAGTT GCAGAATGTA AAAGAGAAAG AAATTCCATT CAAGAAGTTG 
AATGCCAAAG TCTTACAAAT GTCATCCAAG AATATTGGTT CTGAGCAACA ATCCAAAGCC 
CAGGAAGCCA CGGAAAACAA AAGGGAGGCA GCTCTAGAGC ATGAAAAGCA GATGTTGGGC 
ACTGTGTTAA ATAATAAAGA GCTTCAAGAC AGAACTTCCC ATGATATTAA AACTCCAGTT 
ACAGTTGGGG CTTCTAAAAG TGGGGTTAAC CCTGCTCTGC CGGTCTTCAA AAGTATATCC 
CCTAGACCCT TGAAACAGCA AGGCTTGGCC CCTCCTAGAT CTTTACCTCC AGCTTTGACT 
CTTCAGTCAC ATCATCTTGA AAACTTGAGG AAGACTCAGA GCTCTATTGT ACCTAACAGA 
GAATCAAACT CTGCACCCTA CCAACACAAT CAGGAGAGGA TCCGGATTCT CGAGGCCACT 
GCTCAGATGC TGAAAGAGAG AATTGAATTT CTGACTGAGA AGTTAAACCT CTCAGGAGTA 
TGTCTGACAC TTAATGGCAA ATCTGAGAAG TGCATGGATG TACAAAGCAC CAATATCTCA 
TCAGTTCAGG AGTGCCCCAC AATGATAGTA TCATCTTGTT CTCTTGTACT TGGGCAGTCT 
TCCAGCCCAA CTGCCTTGGG AACCCCTGGA GGTAATGAAC CAGAAGTGAA CAAGCCCCTC 
AGTGACAGAG AGAAAGAGAT GACTCAGAAA GGTGGAAAAG ATGGAGTGGA AATGGAAACA 
AAGGTCCCAG CTGTTCTCAG CCCTGCTGCA ACTGCTGAAA TGTTATCCTG TAGAAGCCCA 
CGATTGAATA AGCAACCAAG TCCTTGCCTT GAGGAAGTAA TGAAACCTGT ACCTGATGAT 
TTTACTCTGA CTGAGGATTC TGAAATGGAA GAGTGGGCAC CAGAAGATAA ATTCACCTGT 
ACTCCTACTT TCTTCACAAG AAGTTCACAG AAAGAATTTG AAAAGCAAGA TAGCTTCATT 
GATCCTTCTG GAATTCAGAC GGAAGAAGAA CTGTCTGAAT TTTCTCTTGG AATCTCTGAA 
TCCTATAATG ACATCAAGAT ACGAGACTTT CCCACCAGAA GAAATGAGTT TTCAAGCTCT 
TCAAGCAAAC ATAGGGAGGA ATATGAAAGT TGCAACAAAT ATTCCAAAGA TGTCTGCATG 
GATTATTTAA GCAGCATGCA ACAGAAATCT CTTAACTTTC TTCAGAAAAT GAAGTTACAT 
CAGATAAAAC AGGAAAGAGA GTTAGAAATT CTGAAGCATA GAGCAGAACT GGAAGCTGCA 
GAAACTCAGA AATCTTTGGA TGAATTGGTC TTTAGGAATC ATTTGAAGCA GTCTTTGCAC 
GGCAGCCACA TCCATAGAAC TGAACTAGAC AATAAAGCAG AGAAATGGAA GATGCCAAAG 
ACCCATGGAG GCCTGGAATC CACAGACTGT CTAACTGGGC TGTCATCTAT GGGCAGGTCA 
TATTCCAGCA GAACCAGTAG TCCAACAGTT TCCACCTGGG AAAGTGAGCC AAGGAGAAAT 
TTATTGGGTT GCACAGATGC TGATAAGGAA AGAGGGAAGA AGCAAGACTA TACCACATCA 
TCACACTCCA CTACATGGTT CTCATCAGCT TTTGGTTATC CAAGTAGCAA GGTATTCTCT 
AAAGAAGACT CCATGGACAG CAGCAGCCAG AGGAATGAAA TCTATCGGTC TAGCCTCTTC 
GACCGCTTTA GCCTGAGGAT GACTGATCAG TATTTAAAGG AAGAAGAGCT GAGAGCACAG 
TACCAGATAG CCCTCCTCAA GCTCCGGGAG AAAGCCCTAG AGGAGAAAGT TATAGCTGAA 
TTGTCATGGC TAGAGCAACA GAAAGCGTGT TTGGGGAACA AAAGAGATCA CAGCTTAATG 
ACTGAGGAAC AGCACCAAAT ACTAATAGAT CTGAAGAAAG AACAGTTTTC TAGTCCCTGG 
TCAGAAGCTA TAGAAAGCCA GAAGTCTGAA GATGAGAGAA TCTTCAGATT TGAGAGGTTG 
GTTAGCAGTC CAGAGGGTTT GTTGTCTTCA ACATCATCTC ATACAGAAAG ATCCTCTTCA 
GTCTTGCCTG ACCTTGGGGA AAACCTGAAA TGCTATGAGA ACACACACCT TCTTGCTGAG 
AGGGAGATCC AGGGGAAACC TGATGAGGAC TCATGCCTAC AGCCACAGTG GCTGAAGGGG 
GGAAAGGACA GATCAATGGT CAGTAACATA CCTGACCACA GGGAGCAAAC TCTGGGCCAG 
TTCGTGAAGA ATGACAACCA GGAAAATAAA GAACCTGCTG AACAGCACTG TACTAGCTGG 
GCATGTCGAC AGCAAGAAAG CTCTTTGGAA ATCAAACAAG ATCCGTATCA GGTCTTTGCT 
AAGCCAACTA CAAGGACAGA GGAATATAAT TCAAAGACTG ACTTCAAGCC TAATATGGTG 
AAAAAAAGAC CTCAAAGTGA AGTAATAGGC TCATTAGAAA ATGGTGAGAG ATACATGCAA 
GGACAAAAAA ATGAACTAAA TTTTTTAAAA TTGGAAAGAA GTTTGGCTGG ACAAGAAGGT 
GGTCAATCTT TTCAGCAGGA AGACAGTGTT ATTTACAGTA GAAAAAATCA GGAGACCTCA 
GGACTGGACT CAGATGTCAT TAAGAGTCCA ATAGTTGAAC CAGAAGAGGA CAAAAGAAGG 
AAACAGAGGA TGAAGGGTAA ATCTCAAGAA GTTCCATTGA AAACTATCCA GATATGTCAC 
ACTTCTGCCA TTCAAGAATG CAAAAAAGAT CAAGAGAAGA AATCAGAGTA TACTCAATAT 
GATGAAAACC ACTTGCATAT TAAGTGTGAT ACGACTTCTG ACTCCTTAAA ATCAGGATCT 
GTTTTCAGTA CTTCTTTAGA ATCTCTTTCT TCAGACACAA TATCACCATG CAACTCACCT 
CTAGATTTAA AGAGTGATCA GTCATATACA TCTTTTTCTG AATTTCAGAA AGTGACTGCC 
TTTCTAGTTA ATGTTTCAGA CAGTTTGATT TCAGGAAGTG ACTCAGAAAC TGAGAGTTCA 
CAGAACACAG ATGTCAATGA ATGGCAGGAG TTCACTGACC AACAACCATT GAATGAGCTT 
TCTAGTCCTT TGCCTAATCC ATCAGTAACT CCAAGGAAAG GAGATGAATT GATTTTGGCA 
GTTAATAGTG AAGAGAAACA GCCAGATAGA GATTTCCCTT TTGAAAACTG CCAAGACTTA 
CAGACACAAG GGAATAATAA TATATCATCT AATGACTCTC CTTCTGAAAC AAATAATGTA 
AGTTTGTCAG GCAGCAGGAA ACACAAGCTA AGCCTTTCGT GGACAGAGTT ACCATTGTCT 
CAAGATTCAT CCTCTGGAAA GCTGAGTCAT ATTCTTTCAG AGGATACCTA TGTGGCACCA 
AGACAGGATA AAGAAAAGAG TTGCTCGGAG CATAAAGTTT GTGTTGAACC CAAGCTCAGT 
TCCTTTTCAT TCTTGGATGA CTTTTCAAAG ACAGATATAG ATTTGAAGCA GCAAGGTCCT 
ACAATTTCCC TGAATGAAGG AAAAGATCAG GAAGACCTTG AAATCAAAGA TGTTTTAGCG 
GATGGAACAA ACAAAAAAGA AACTGGATTT TCTCTCCCAG AAATTTATTA CATGTCATTT 
CCATCCCCAA ATCTGAAAGC AAATTGCCAA TCACCTTCAC TGCCTCTTAA AATGTCACAG 
AGAAAACAAA ACTCGTTAGT CAAAAAAGCT AAAGCTGCAT GTGAAGAAAA TCCAGAAGAA 
AGGCTTATTT CTAATAAAGA GTGTAAATAT GCCATTCAGC AGTTGTCAAT TGATTTCAAC 
AGTATGCCTG CTATACCTGG CCCCCAGATC TTTCATGATG GAAAAATTGT TCAGTCAGTA 
TATGAGCACA AGGCAGATAA ATCTGATATT CATCACAGGA CCTCAGAGGG CCACGAGGTG 
GTGATATCTC CTTCTGGATC ACTTAGTGGG CTGATTCCTG AGGCTGAGCA TTGCCCAGCT 
ATCAGGGTTG ATGGTTCCAC ACATACTGAA AGAGATGGTC TACCTAGTGT CCAAGATAGC 
ATCTTGTCTA AAATACTATC CCCTGTCGAC GAAGTGCTTT CTTATGGAAG TGCTGGCCTT 
CCATCCCCAA CCCAGAAGGT TTCATCTTTC GCCAGTGAAA ATCTCCCTTC TCCTCCTCCT 
GACTTTGTGC TTTGGGCAAA TGTCAGTGAT ATTGACTTCA ATTCAGAAGA CTTCCCTTCT 
CTGCCAGAAG AAATGGTATT TCCAGAAGAT TCCAAGGATG TACAAGATGA AGATTCATCT 
ATTCAAACTG GAGAGTTATC TTCTTTATCT GATGAGATAC TGCTGGAGGA ATTTTCTCCA 
ATACCTTTAG ATGTAGGGAA TTCCTGCTCA GTTCTGGATG AACACTCCCT AGGATGGGAC 
AAAGAGAGAG AAAGAAGTTT GGAAAGGAAG GAGGAATTGT TGAGAGTCGA ACCACAGCAG 
GATATCAAAA GGAAGCAAAA AGTGGGCTGC TGGTACAGTC CTGCTTTGTC CAGAAGTGCC 
AGTAAAAGTC CAGCATCATT CTTCAAACTA TCCACAGATA AAGATGGCAA GAATCCACTG 
GCATTGGAGG ATGCAGAGAA CAGAGAACAT ACACAACCAG AAACTCTGAA ATTCAGAGAG 
ACAAGCCATT TTGAAGGAAA ACACTGGACT AGTTTGGTAT CCGATGAAGC AAAGAATGAT 
CATCACAAAG AAGACTTGGA AAGAGTAAAA CATTCAGAGT ATGACATGGC TTATGATGAG 
TTATTTAGAT CAGATAAAAT GTCATACTTT TTGGCAGACG ACATAGAAAA GCCAAGTGAC 
CATAATTACC AAGATTCAGT TCATGATGAA TCATCCCTAA GGAGTAACAA GTGCTATGAT 
GAGGTCAGAG TGGAATACCG GTTTTTAGAG AGAAAGCCAG AAATAAAGTT TAACAATAGA 
GATAGCTCAA TGACAACAGT AGCCGCTTAT CCTGAAACCC TGATGTTCCA ACCTACAGAG 
ATGGAGTCCT ACTTTATTCC TACTCAGTCT GAGTCTGAAA GATTTTTAAA TAAAGATTTC 
TCAGGCTGCC TAAATAGCCA AAAAGAACAG CCATATATGG CACCTATAAA AAATGAGCCT 
GAGAAGTTAC TTGAAGATGT TCATCCAATG GAAGAGAAAG AAATCAACTT AAAAAATAGT 
GTAATGGGCT CCCAACTTGA TCCTAGGCAA AAAACATATG GAATTGTAGA TTCTGTATCC 
ACTGAACTGA CCAGAAAAAT CCTGTGGGAT GCCTTAGCTG TGTTTGCAGA ACTTGCCAGA 
CAATAGAGTG TCTTCCTGTC CATCAACTGC TGTTGTAACC CCCTGATGAG GGAAAGCTCT 
GGGGTTTTCT ATGAAAAAAC AAAATTGCTA ACAATGCCCT CTCTAATAAC TGCTTTGACA 
AACCACATAT GTGTCAGTGA CACAGAAAAG AGAAAATGGA ATTTTGACAA TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100932056 ( XM_031954950.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031810810.1 Sarcophilus harrisii coiled-coil domain containing 187 (CCDC187), transcript variant X1, mRNA.

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CloneID	OSa226575
Clone ID Related Accession (Same CDS sequence)	XM_031954950.1
Accession Version	XM_031954950.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	coiled-coil domain-containing protein 187 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTTG ACTACATGAC CTCCAATGTT CCTCTCAGTT CTTTGAGCTC CTCCTCTCCA 
TCTGAACAGA TCCAATCAAG GCAGAAAAAC TCTGTCTACC TGAGCCCTCA GTTACAGCGG 
ATGGAGAGTG AAATGGACAC CCCCAGCTGG CACAGCATCA GCAGCCAGAG CTGCGGATCA 
CTTGATTTTG GGCAAGAGAA GCTGTTTTCT TGGGAAAACT GGCACAATGC TAGTCCTGCC 
TATGCCAAAC TGAGAATGCC TCATTTTTCT GAGGACTACA ACATGGCAGC TGCATCAGTG 
TCACCCTGGG TCTTTCGGGA GTCAGTTATC TTCAGAGAAG AACCAGATGG CAAGACTTAC 
GGTGGACGAG TAACTCATTT GAATCCTGTG ATTTTTGGCC CAGTGTGGAA AGAGCAGGAC 
TTTAACTACC CACCTGAACC AGAGAGAATG TGTACAACTA GTGACTGGCC CTCCAAAATG 
CCCGTCTTTA TTCCCGTTCA GTCGGGAAGC CATCATCAGT TCTTGAATCA CCCAAGTGAC 
CCCTGGAGGG AAAAGTCACC TCAGCCAGTT GAGTCTCCCA CTAATTTTCA GAGATGGCCA 
TTGACAGGGG TAAAGAGAGC TTTCCCTGAA TCCCAGCTGG AGCAGCTGAG GGAGAAGATC 
CGGGCACAGA AACATCGACA TGCTAATGTT TTCCCTACCT CCTTCCAAGC TCGCTCTGGG 
ACCCCCCAAA CTCTGCCCTG GAAGCACGTG CCAAAGAGAA GCGTCTCCAA AATGAAGTTA 
GCTGCTTCAA AGCCAGCCCA CCCGGGCCAA GTCTCGGCTT CCAGCCCAGA TCCCTCTGAC 
ACTCACCATG CCGTTTCCCA CAAATTTATA CCAAAGAGAA AGAGCTACAG AGTTAGATTT 
GCATTATCAA CGCCAATAGA CGCACATCAA AGGGATAAAA ATAAAACTAC AGTGACCAAA 
AGCTGCCAGG TATCTTCTCA GTCACTTTCT CTTGGAAAAC AGCTAAAAGG CCAAGATGCT 
AAGGTGACTG GCGTTTCTGC ATGGAGAAAG GGACAGAGGC TTGCGAGGCT CCTCCTTGGG 
CCCCCTCCCA CGTTTCTCCA GCTCCAGAAA AGGGCTCTCT CCGGAGGCCT GGCCGCCACA 
AAGGAGTCAG GACTGACAGA GAAGACTCAA AAATCTGAGC ACATACCACC CTCCACCCGA 
GATATAAACA ACCAGAAAGG AGCAGCTCCT CTCCGTGGGA ACTCCCAGAA CTCTCCAGAA 
AGTTCAGCAC AAGTAGGCTG TGGTGGTTCT GAATCATTCC ATAGGATCTT AAACTTTTTG 
AAAAATCTCC AATCACGTGT TCAAGACAAG GCAGATCACA AAAGAACACG GCCCCAGTCT 
CCAAAGATCG CGTCTACCAA GAAAGCAGGC AAGTCAGAAT GTCATTCAAA ATCTCAAAAT 
GCAGCAGTGA GAAGGAATCT TCAATTTGTC TCAGGAGGAA AGAACCTCTC TTCAAGGATG 
GGTAGTTCTG GGACATCAAA AGCACCTGGA AAAGAAAATG CCTGCCAACG GACTCTCAAA 
AAAACAAATA AGGGTGTCCC TCGTCCCTGT GTCACTGCAG AAATGCAGGA ATTGATGAAT 
CAAAAAGTAT CTGAAAGAAA GAAAAGATGC CTTGAAGCCA AAATATATGT AAAGAAAGCC 
TTGGAGTCAA GAGATAAGTT GCAGAATGTA AAAGAGAAAG AAATTCCATT CAAGAAGTTG 
AATGCCAAAG TCTTACAAAT GTCATCCAAG AATATTGGTT CTGAGCAACA ATCCAAAGCC 
CAGGAAGCCA CGGAAAACAA AAGGGAGGCA GCTCTAGAGC ATGAAAAGCA GATGTTGGGC 
ACTGTGTTAA ATAATAAAGA GCTTCAAGAC AGAACTTCCC ATGATATTAA AACTCCAGTT 
ACAGTTGGGG CTTCTAAAAG TGGGGTTAAC CCTGCTCTGC CGGTCTTCAA AAGTATATCC 
CCTAGACCCT TGAAACAGCA AGGCTTGGCC CCTCCTAGAT CTTTACCTCC AGCTTTGACT 
CTTCAGTCAC ATCATCTTGA AAACTTGAGG AAGACTCAGA GCTCTATTGT ACCTAACAGA 
GAATCAAACT CTGCACCCTA CCAACACAAT CAGGAGAGGA TCCGGATTCT CGAGGCCACT 
GCTCAGATGC TGAAAGAGAG AATTGAATTT CTGACTGAGA AGTTAAACCT CTCAGGAGTA 
TGTCTGACAC TTAATGGCAA ATCTGAGAAG TGCATGGATG TACAAAGCAC CAATATCTCA 
TCAGTTCAGG AGTGCCCCAC AATGATAGTA TCATCTTGTT CTCTTGTACT TGGGCAGTCT 
TCCAGCCCAA CTGCCTTGGG AACCCCTGGA GGTAATGAAC CAGAAGTGAA CAAGCCCCTC 
AGTGACAGAG AGAAAGAGAT GACTCAGAAA GGTGGAAAAG ATGGAGTGGA AATGGAAACA 
AAGGTCCCAG CTGTTCTCAG CCCTGCTGCA ACTGCTGAAA TGTTATCCTG TAGAAGCCCA 
CGATTGAATA AGCAACCAAG TCCTTGCCTT GAGGAAGTAA TGAAACCTGT ACCTGATGAT 
TTTACTCTGA CTGAGGATTC TGAAATGGAA GAGTGGGCAC CAGAAGATAA ATTCACCTGT 
ACTCCTACTT TCTTCACAAG AAGTTCACAG AAAGAATTTG AAAAGCAAGA TAGCTTCATT 
GATCCTTCTG GAATTCAGAC GGAAGAAGAA CTGTCTGAAT TTTCTCTTGG AATCTCTGAA 
TCCTATAATG ACATCAAGAT ACGAGACTTT CCCACCAGAA GAAATGAGTT TTCAAGCTCT 
TCAAGCAAAC ATAGGGAGGA ATATGAAAGT TGCAACAAAT ATTCCAAAGA TGTCTGCATG 
GATTATTTAA GCAGCATGCA ACAGAAATCT CTTAACTTTC TTCAGAAAAT GAAGTTACAT 
CAGATAAAAC AGGAAAGAGA GTTAGAAATT CTGAAGCATA GAGCAGAACT GGAAGCTGCA 
GAAACTCAGA AATCTTTGGA TGAATTGGTC TTTAGGAATC ATTTGAAGGT GAGTCCTAAG 
CAGTCTTTGC ACGGCAGCCA CATCCATAGA ACTGAACTAG ACAATAAAGC AGAGAAATGG 
AAGATGCCAA AGACCCATGG AGGCCTGGAA TCCACAGACT GTCTAACTGG GCTGTCATCT 
ATGGGCAGGT CATATTCCAG CAGAACCAGT AGTCCAACAG TTTCCACCTG GGAAAGTGAG 
CCAAGGAGAA ATTTATTGGG TTGCACAGAT GCTGATAAGG AAAGAGGGAA GAAGCAAGAC 
TATACCACAT CATCACACTC CACTACATGG TTCTCATCAG CTTTTGGTTA TCCAAGTAGC 
AAGGTATTCT CTAAAGAAGA CTCCATGGAC AGCAGCAGCC AGAGGAATGA AATCTATCGG 
TCTAGCCTCT TCGACCGCTT TAGCCTGAGG ATGACTGATC AGTATTTAAA GGAAGAAGAG 
CTGAGAGCAC AGTACCAGAT AGCCCTCCTC AAGCTCCGGG AGAAAGCCCT AGAGGAGAAA 
GTTATAGCTG AATTGTCATG GCTAGAGCAA CAGAAAGCGT GTTTGGGGAA CAAAAGAGAT 
CACAGCTTAA TGACTGAGGA ACAGCACCAA ATACTAATAG ATCTGAAGAA AGAACAGGCG 
GAACTTAAAC ACCTACAGAA CATTTATGAA ATGGCTCATC AGGAGAGAAA ATTTTTGTTG 
ACACAACAAA AGGACCTCTC AACAATACAG CAGTCAACAA GTGATCTTCA GTCGGAATTT 
ACTGTCCTAG CCAGCAGACA CCAGTTTTCT AGTCCCTGGT CAGAAGCTAT AGAAAGCCAG 
AAGTCTGAAG ATGAGAGAAT CTTCAGATTT GAGAGGTTGG TTAGCAGTCC AGAGGGTTTG 
TTGTCTTCAA CATCATCTCA TACAGAAAGA TCCTCTTCAG TCTTGCCTGA CCTTGGGGAA 
AACCTGAAAT GCTATGAGAA CACACACCTT CTTGCTGAGA GGGAGATCCA GGGGAAACCT 
GATGAGGACT CATGCCTACA GCCACAGTGG CTGAAGGGGG GAAAGGACAG ATCAATGGTC 
AGTAACATAC CTGACCACAG GGAGCAAACT CTGGGCCAGT TCGTGAAGAA TGACAACCAG 
GAAAATAAAG AACCTGCTGA ACAGCACTGT ACTAGCTGGG CATGTCGACA GCAAGAAAGC 
TCTTTGGAAA TCAAACAAGA TCCGTATCAG GTCTTTGCTA AGCCAACTAC AAGGACAGAG 
GAATATAATT CAAAGACTGA CTTCAAGCCT AATATGGTGA AAAAAAGACC TCAAAGTGAA 
GTAATAGGCT CATTAGAAAA TGGTGAGAGA TACATGCAAG GACAAAAAAA TGAACTAAAT 
TTTTTAAAAT TGGAAAGAAG TTTGGCTGGA CAAGAAGGTG GTCAATCTTT TCAGCAGGAA 
GACAGTGTTA TTTACAGTAG AAAAAATCAG GAGACCTCAG GACTGGACTC AGATGTCATT 
AAGAGTCCAA TAGTTGAACC AGAAGAGGAC AAAAGAAGGA AACAGAGGAT GAAGGGTAAA 
TCTCAAGAAG TTCCATTGAA AACTATCCAG ATATGTCACA CTTCTGCCAT TCAAGAATGC 
AAAAAAGATC AAGAGAAGAA ATCAGAGTAT ACTCAATATG ATGAAAACCA CTTGCATATT 
AAGTGTGATA CGACTTCTGA CTCCTTAAAA TCAGGATCTG TTTTCAGTAC TTCTTTAGAA 
TCTCTTTCTT CAGACACAAT ATCACCATGC AACTCACCTC TAGATTTAAA GAGTGATCAG 
TCATATACAT CTTTTTCTGA ATTTCAGAAA GTGACTGCCT TTCTAGTTAA TGTTTCAGAC 
AGTTTGATTT CAGGAAGTGA CTCAGAAACT GAGAGTTCAC AGAACACAGA TGTCAATGAA 
TGGCAGGAGT TCACTGACCA ACAACCATTG AATGAGCTTT CTAGTCCTTT GCCTAATCCA 
TCAGTAACTC CAAGGAAAGG AGATGAATTG ATTTTGGCAG TTAATAGTGA AGAGAAACAG 
CCAGATAGAG ATTTCCCTTT TGAAAACTGC CAAGACTTAC AGACACAAGG GAATAATAAT 
ATATCATCTA ATGACTCTCC TTCTGAAACA AATAATGTAA GTTTGTCAGG CAGCAGGAAA 
CACAAGCTAA GCCTTTCGTG GACAGAGTTA CCATTGTCTC AAGATTCATC CTCTGGAAAG 
CTGAGTCATA TTCTTTCAGA GGATACCTAT GTGGCACCAA GACAGGATAA AGAAAAGAGT 
TGCTCGGAGC ATAAAGTTTG TGTTGAACCC AAGCTCAGTT CCTTTTCATT CTTGGATGAC 
TTTTCAAAGA CAGATATAGA TTTGAAGCAG CAAGGTCCTA CAATTTCCCT GAATGAAGGA 
AAAGATCAGG AAGACCTTGA AATCAAAGAT GTTTTAGCGG ATGGAACAAA CAAAAAAGAA 
ACTGGATTTT CTCTCCCAGA AATTTATTAC ATGTCATTTC CATCCCCAAA TCTGAAAGCA 
AATTGCCAAT CACCTTCACT GCCTCTTAAA ATGTCACAGA GAAAACAAAA CTCGTTAGTC 
AAAAAAGCTA AAGCTGCATG TGAAGAAAAT CCAGAAGAAA GGCTTATTTC TAATAAAGAG 
TGTAAATATG CCATTCAGCA GTTGTCAATT GATTTCAACA GTATGCCTGC TATACCTGGC 
CCCCAGATCT TTCATGATGG AAAAATTGTT CAGTCAGTAT ATGAGCACAA GGCAGATAAA 
TCTGATATTC ATCACAGGAC CTCAGAGGGC CACGAGGTGG TGATATCTCC TTCTGGATCA 
CTTAGTGGGC TGATTCCTGA GGCTGAGCAT TGCCCAGCTA TCAGGGTTGA TGGTTCCACA 
CATACTGAAA GAGATGGTCT ACCTAGTGTC CAAGATAGCA TCTTGTCTAA AATACTATCC 
CCTGTCGACG AAGTGCTTTC TTATGGAAGT GCTGGCCTTC CATCCCCAAC CCAGAAGGTT 
TCATCTTTCG CCAGTGAAAA TCTCCCTTCT CCTCCTCCTG ACTTTGTGCT TTGGGCAAAT 
GTCAGTGATA TTGACTTCAA TTCAGAAGAC TTCCCTTCTC TGCCAGAAGA AATGGTATTT 
CCAGAAGATT CCAAGGATGT ACAAGATGAA GATTCATCTA TTCAAACTGG AGAGTTATCT 
TCTTTATCTG ATGAGATACT GCTGGAGGAA TTTTCTCCAA TACCTTTAGA TGTAGGGAAT 
TCCTGCTCAG TTCTGGATGA ACACTCCCTA GGATGGGACA AAGAGAGAGA AAGAAGTTTG 
GAAAGGAAGG AGGAATTGTT GAGAGTCGAA CCACAGCAGG ATATCAAAAG GAAGCAAAAA 
GTGGGCTGCT GGTACAGTCC TGCTTTGTCC AGAAGTGCCA GTAAAAGTCC AGCATCATTC 
TTCAAACTAT CCACAGATAA AGATGGCAAG AATCCACTGG CATTGGAGGA TGCAGAGAAC 
AGAGAACATA CACAACCAGA AACTCTGAAA TTCAGAGAGA CAAGCCATTT TGAAGGAAAA 
CACTGGACTA GTTTGGTATC CGATGAAGCA AAGAATGATC ATCACAAAGA AGACTTGGAA 
AGAGTAAAAC ATTCAGAGTA TGACATGGCT TATGATGAGT TATTTAGATC AGATAAAATG 
TCATACTTTT TGGCAGACGA CATAGAAAAG CCAAGTGACC ATAATTACCA AGATTCAGTT 
CATGATGAAT CATCCCTAAG GAGTAACAAG TGCTATGATG AGGTCAGAGT GGAATACCGG 
TTTTTAGAGA GAAAGCCAGA AATAAAGTTT AACAATAGAG ATAGCTCAAT GACAACAGTA 
GCCGCTTATC CTGAAACCCT GATGTTCCAA CCTACAGAGA TGGAGTCCTA CTTTATTCCT 
ACTCAGTCTG AGTCTGAAAG ATTTTTAAAT AAAGATTTCT CAGGCTGCCT AAATAGCCAA 
AAAGAACAGC CATATATGGC ACCTATAAAA AATGAGCCTG AGAAGTTACT TGAAGATGTT 
CATCCAATGG AAGAGAAAGA AATCAACTTA AAAAATAGTG TAATGGGCTC CCAACTTGAT 
CCTAGGCAAA AAACATATGG AATTGTAGAT TCTGTATCCA CTGAACTGAC CAGAAAAATC 
CTGTGGGATG CCTTAGCTGT GTTTGCAGAA CTTGCCAGAC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031810810.1
CDS	310..7494
Translation	MVFDYMTSNVPLSSLSSSSPSEQIQSRQKNSVYLSPQLQRMESEMDTPSWHSISSQSCGSLDFGQEKLFSWENWHNASPAYAKLRMPHFSEDYNMAAASVSPWVFRESVIFREEPDGKTYGGRVTHLNPVIFGPVWKEQDFNYPPEPERMCTTSDWPSKMPVFIPVQSGSHHQFLNHPSDPWREKSPQPVESPTNFQRWPLTGVKRAFPESQLEQLREKIRAQKHRHANVFPTSFQARSGTPQTLPWKHVPKRSVSKMKLAASKPAHPGQVSASSPDPSDTHHAVSHKFIPKRKSYRVRFALSTPIDAHQRDKNKTTVTKSCQVSSQSLSLGKQLKGQDAKVTGVSAWRKGQRLARLLLGPPPTFLQLQKRALSGGLAATKESGLTEKTQKSEHIPPSTRDINNQKGAAPLRGNSQNSPESSAQVGCGGSESFHRILNFLKNLQSRVQDKADHKRTRPQSPKIASTKKAGKSECHSKSQNAAVRRNLQFVSGGKNLSSRMGSSGTSKAPGKENACQRTLKKTNKGVPRPCVTAEMQELMNQKVSERKKRCLEAKIYVKKALESRDKLQNVKEKEIPFKKLNAKVLQMSSKNIGSEQQSKAQEATENKREAALEHEKQMLGTVLNNKELQDRTSHDIKTPVTVGASKSGVNPALPVFKSISPRPLKQQGLAPPRSLPPALTLQSHHLENLRKTQSSIVPNRESNSAPYQHNQERIRILEATAQMLKERIEFLTEKLNLSGVCLTLNGKSEKCMDVQSTNISSVQECPTMIVSSCSLVLGQSSSPTALGTPGGNEPEVNKPLSDREKEMTQKGGKDGVEMETKVPAVLSPAATAEMLSCRSPRLNKQPSPCLEEVMKPVPDDFTLTEDSEMEEWAPEDKFTCTPTFFTRSSQKEFEKQDSFIDPSGIQTEEELSEFSLGISESYNDIKIRDFPTRRNEFSSSSSKHREEYESCNKYSKDVCMDYLSSMQQKSLNFLQKMKLHQIKQERELEILKHRAELEAAETQKSLDELVFRNHLKVSPKQSLHGSHIHRTELDNKAEKWKMPKTHGGLESTDCLTGLSSMGRSYSSRTSSPTVSTWESEPRRNLLGCTDADKERGKKQDYTTSSHSTTWFSSAFGYPSSKVFSKEDSMDSSSQRNEIYRSSLFDRFSLRMTDQYLKEEELRAQYQIALLKLREKALEEKVIAELSWLEQQKACLGNKRDHSLMTEEQHQILIDLKKEQAELKHLQNIYEMAHQERKFLLTQQKDLSTIQQSTSDLQSEFTVLASRHQFSSPWSEAIESQKSEDERIFRFERLVSSPEGLLSSTSSHTERSSSVLPDLGENLKCYENTHLLAEREIQGKPDEDSCLQPQWLKGGKDRSMVSNIPDHREQTLGQFVKNDNQENKEPAEQHCTSWACRQQESSLEIKQDPYQVFAKPTTRTEEYNSKTDFKPNMVKKRPQSEVIGSLENGERYMQGQKNELNFLKLERSLAGQEGGQSFQQEDSVIYSRKNQETSGLDSDVIKSPIVEPEEDKRRKQRMKGKSQEVPLKTIQICHTSAIQECKKDQEKKSEYTQYDENHLHIKCDTTSDSLKSGSVFSTSLESLSSDTISPCNSPLDLKSDQSYTSFSEFQKVTAFLVNVSDSLISGSDSETESSQNTDVNEWQEFTDQQPLNELSSPLPNPSVTPRKGDELILAVNSEEKQPDRDFPFENCQDLQTQGNNNISSNDSPSETNNVSLSGSRKHKLSLSWTELPLSQDSSSGKLSHILSEDTYVAPRQDKEKSCSEHKVCVEPKLSSFSFLDDFSKTDIDLKQQGPTISLNEGKDQEDLEIKDVLADGTNKKETGFSLPEIYYMSFPSPNLKANCQSPSLPLKMSQRKQNSLVKKAKAACEENPEERLISNKECKYAIQQLSIDFNSMPAIPGPQIFHDGKIVQSVYEHKADKSDIHHRTSEGHEVVISPSGSLSGLIPEAEHCPAIRVDGSTHTERDGLPSVQDSILSKILSPVDEVLSYGSAGLPSPTQKVSSFASENLPSPPPDFVLWANVSDIDFNSEDFPSLPEEMVFPEDSKDVQDEDSSIQTGELSSLSDEILLEEFSPIPLDVGNSCSVLDEHSLGWDKERERSLERKEELLRVEPQQDIKRKQKVGCWYSPALSRSASKSPASFFKLSTDKDGKNPLALEDAENREHTQPETLKFRETSHFEGKHWTSLVSDEAKNDHHKEDLERVKHSEYDMAYDELFRSDKMSYFLADDIEKPSDHNYQDSVHDESSLRSNKCYDEVRVEYRFLERKPEIKFNNRDSSMTTVAAYPETLMFQPTEMESYFIPTQSESERFLNKDFSGCLNSQKEQPYMAPIKNEPEKLLEDVHPMEEKEINLKNSVMGSQLDPRQKTYGIVDSVSTELTRKILWDALAVFAELARQ

Target ORF information:

RefSeq Version	XM_031954950.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii coiled-coil domain containing 187 (CCDC187), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031954950.1

ORF Insert Sequence:

ATGGTATTTG ACTACATGAC CTCCAATGTT CCTCTCAGTT CTTTGAGCTC CTCCTCTCCA 
TCTGAACAGA TCCAATCAAG GCAGAAAAAC TCTGTCTACC TGAGCCCTCA GTTACAGCGG 
ATGGAGAGTG AAATGGACAC CCCCAGCTGG CACAGCATCA GCAGCCAGAG CTGCGGATCA 
CTTGATTTTG GGCAAGAGAA GCTGTTTTCT TGGGAAAACT GGCACAATGC TAGTCCTGCC 
TATGCCAAAC TGAGAATGCC TCATTTTTCT GAGGACTACA ACATGGCAGC TGCATCAGTG 
TCACCCTGGG TCTTTCGGGA GTCAGTTATC TTCAGAGAAG AACCAGATGG CAAGACTTAC 
GGTGGACGAG TAACTCATTT GAATCCTGTG ATTTTTGGCC CAGTGTGGAA AGAGCAGGAC 
TTTAACTACC CACCTGAACC AGAGAGAATG TGTACAACTA GTGACTGGCC CTCCAAAATG 
CCCGTCTTTA TTCCCGTTCA GTCGGGAAGC CATCATCAGT TCTTGAATCA CCCAAGTGAC 
CCCTGGAGGG AAAAGTCACC TCAGCCAGTT GAGTCTCCCA CTAATTTTCA GAGATGGCCA 
TTGACAGGGG TAAAGAGAGC TTTCCCTGAA TCCCAGCTGG AGCAGCTGAG GGAGAAGATC 
CGGGCACAGA AACATCGACA TGCTAATGTT TTCCCTACCT CCTTCCAAGC TCGCTCTGGG 
ACCCCCCAAA CTCTGCCCTG GAAGCACGTG CCAAAGAGAA GCGTCTCCAA AATGAAGTTA 
GCTGCTTCAA AGCCAGCCCA CCCGGGCCAA GTCTCGGCTT CCAGCCCAGA TCCCTCTGAC 
ACTCACCATG CCGTTTCCCA CAAATTTATA CCAAAGAGAA AGAGCTACAG AGTTAGATTT 
GCATTATCAA CGCCAATAGA CGCACATCAA AGGGATAAAA ATAAAACTAC AGTGACCAAA 
AGCTGCCAGG TATCTTCTCA GTCACTTTCT CTTGGAAAAC AGCTAAAAGG CCAAGATGCT 
AAGGTGACTG GCGTTTCTGC ATGGAGAAAG GGACAGAGGC TTGCGAGGCT CCTCCTTGGG 
CCCCCTCCCA CGTTTCTCCA GCTCCAGAAA AGGGCTCTCT CCGGAGGCCT GGCCGCCACA 
AAGGAGTCAG GACTGACAGA GAAGACTCAA AAATCTGAGC ACATACCACC CTCCACCCGA 
GATATAAACA ACCAGAAAGG AGCAGCTCCT CTCCGTGGGA ACTCCCAGAA CTCTCCAGAA 
AGTTCAGCAC AAGTAGGCTG TGGTGGTTCT GAATCATTCC ATAGGATCTT AAACTTTTTG 
AAAAATCTCC AATCACGTGT TCAAGACAAG GCAGATCACA AAAGAACACG GCCCCAGTCT 
CCAAAGATCG CGTCTACCAA GAAAGCAGGC AAGTCAGAAT GTCATTCAAA ATCTCAAAAT 
GCAGCAGTGA GAAGGAATCT TCAATTTGTC TCAGGAGGAA AGAACCTCTC TTCAAGGATG 
GGTAGTTCTG GGACATCAAA AGCACCTGGA AAAGAAAATG CCTGCCAACG GACTCTCAAA 
AAAACAAATA AGGGTGTCCC TCGTCCCTGT GTCACTGCAG AAATGCAGGA ATTGATGAAT 
CAAAAAGTAT CTGAAAGAAA GAAAAGATGC CTTGAAGCCA AAATATATGT AAAGAAAGCC 
TTGGAGTCAA GAGATAAGTT GCAGAATGTA AAAGAGAAAG AAATTCCATT CAAGAAGTTG 
AATGCCAAAG TCTTACAAAT GTCATCCAAG AATATTGGTT CTGAGCAACA ATCCAAAGCC 
CAGGAAGCCA CGGAAAACAA AAGGGAGGCA GCTCTAGAGC ATGAAAAGCA GATGTTGGGC 
ACTGTGTTAA ATAATAAAGA GCTTCAAGAC AGAACTTCCC ATGATATTAA AACTCCAGTT 
ACAGTTGGGG CTTCTAAAAG TGGGGTTAAC CCTGCTCTGC CGGTCTTCAA AAGTATATCC 
CCTAGACCCT TGAAACAGCA AGGCTTGGCC CCTCCTAGAT CTTTACCTCC AGCTTTGACT 
CTTCAGTCAC ATCATCTTGA AAACTTGAGG AAGACTCAGA GCTCTATTGT ACCTAACAGA 
GAATCAAACT CTGCACCCTA CCAACACAAT CAGGAGAGGA TCCGGATTCT CGAGGCCACT 
GCTCAGATGC TGAAAGAGAG AATTGAATTT CTGACTGAGA AGTTAAACCT CTCAGGAGTA 
TGTCTGACAC TTAATGGCAA ATCTGAGAAG TGCATGGATG TACAAAGCAC CAATATCTCA 
TCAGTTCAGG AGTGCCCCAC AATGATAGTA TCATCTTGTT CTCTTGTACT TGGGCAGTCT 
TCCAGCCCAA CTGCCTTGGG AACCCCTGGA GGTAATGAAC CAGAAGTGAA CAAGCCCCTC 
AGTGACAGAG AGAAAGAGAT GACTCAGAAA GGTGGAAAAG ATGGAGTGGA AATGGAAACA 
AAGGTCCCAG CTGTTCTCAG CCCTGCTGCA ACTGCTGAAA TGTTATCCTG TAGAAGCCCA 
CGATTGAATA AGCAACCAAG TCCTTGCCTT GAGGAAGTAA TGAAACCTGT ACCTGATGAT 
TTTACTCTGA CTGAGGATTC TGAAATGGAA GAGTGGGCAC CAGAAGATAA ATTCACCTGT 
ACTCCTACTT TCTTCACAAG AAGTTCACAG AAAGAATTTG AAAAGCAAGA TAGCTTCATT 
GATCCTTCTG GAATTCAGAC GGAAGAAGAA CTGTCTGAAT TTTCTCTTGG AATCTCTGAA 
TCCTATAATG ACATCAAGAT ACGAGACTTT CCCACCAGAA GAAATGAGTT TTCAAGCTCT 
TCAAGCAAAC ATAGGGAGGA ATATGAAAGT TGCAACAAAT ATTCCAAAGA TGTCTGCATG 
GATTATTTAA GCAGCATGCA ACAGAAATCT CTTAACTTTC TTCAGAAAAT GAAGTTACAT 
CAGATAAAAC AGGAAAGAGA GTTAGAAATT CTGAAGCATA GAGCAGAACT GGAAGCTGCA 
GAAACTCAGA AATCTTTGGA TGAATTGGTC TTTAGGAATC ATTTGAAGGT GAGTCCTAAG 
CAGTCTTTGC ACGGCAGCCA CATCCATAGA ACTGAACTAG ACAATAAAGC AGAGAAATGG 
AAGATGCCAA AGACCCATGG AGGCCTGGAA TCCACAGACT GTCTAACTGG GCTGTCATCT 
ATGGGCAGGT CATATTCCAG CAGAACCAGT AGTCCAACAG TTTCCACCTG GGAAAGTGAG 
CCAAGGAGAA ATTTATTGGG TTGCACAGAT GCTGATAAGG AAAGAGGGAA GAAGCAAGAC 
TATACCACAT CATCACACTC CACTACATGG TTCTCATCAG CTTTTGGTTA TCCAAGTAGC 
AAGGTATTCT CTAAAGAAGA CTCCATGGAC AGCAGCAGCC AGAGGAATGA AATCTATCGG 
TCTAGCCTCT TCGACCGCTT TAGCCTGAGG ATGACTGATC AGTATTTAAA GGAAGAAGAG 
CTGAGAGCAC AGTACCAGAT AGCCCTCCTC AAGCTCCGGG AGAAAGCCCT AGAGGAGAAA 
GTTATAGCTG AATTGTCATG GCTAGAGCAA CAGAAAGCGT GTTTGGGGAA CAAAAGAGAT 
CACAGCTTAA TGACTGAGGA ACAGCACCAA ATACTAATAG ATCTGAAGAA AGAACAGGCG 
GAACTTAAAC ACCTACAGAA CATTTATGAA ATGGCTCATC AGGAGAGAAA ATTTTTGTTG 
ACACAACAAA AGGACCTCTC AACAATACAG CAGTCAACAA GTGATCTTCA GTCGGAATTT 
ACTGTCCTAG CCAGCAGACA CCAGTTTTCT AGTCCCTGGT CAGAAGCTAT AGAAAGCCAG 
AAGTCTGAAG ATGAGAGAAT CTTCAGATTT GAGAGGTTGG TTAGCAGTCC AGAGGGTTTG 
TTGTCTTCAA CATCATCTCA TACAGAAAGA TCCTCTTCAG TCTTGCCTGA CCTTGGGGAA 
AACCTGAAAT GCTATGAGAA CACACACCTT CTTGCTGAGA GGGAGATCCA GGGGAAACCT 
GATGAGGACT CATGCCTACA GCCACAGTGG CTGAAGGGGG GAAAGGACAG ATCAATGGTC 
AGTAACATAC CTGACCACAG GGAGCAAACT CTGGGCCAGT TCGTGAAGAA TGACAACCAG 
GAAAATAAAG AACCTGCTGA ACAGCACTGT ACTAGCTGGG CATGTCGACA GCAAGAAAGC 
TCTTTGGAAA TCAAACAAGA TCCGTATCAG GTCTTTGCTA AGCCAACTAC AAGGACAGAG 
GAATATAATT CAAAGACTGA CTTCAAGCCT AATATGGTGA AAAAAAGACC TCAAAGTGAA 
GTAATAGGCT CATTAGAAAA TGGTGAGAGA TACATGCAAG GACAAAAAAA TGAACTAAAT 
TTTTTAAAAT TGGAAAGAAG TTTGGCTGGA CAAGAAGGTG GTCAATCTTT TCAGCAGGAA 
GACAGTGTTA TTTACAGTAG AAAAAATCAG GAGACCTCAG GACTGGACTC AGATGTCATT 
AAGAGTCCAA TAGTTGAACC AGAAGAGGAC AAAAGAAGGA AACAGAGGAT GAAGGGTAAA 
TCTCAAGAAG TTCCATTGAA AACTATCCAG ATATGTCACA CTTCTGCCAT TCAAGAATGC 
AAAAAAGATC AAGAGAAGAA ATCAGAGTAT ACTCAATATG ATGAAAACCA CTTGCATATT 
AAGTGTGATA CGACTTCTGA CTCCTTAAAA TCAGGATCTG TTTTCAGTAC TTCTTTAGAA 
TCTCTTTCTT CAGACACAAT ATCACCATGC AACTCACCTC TAGATTTAAA GAGTGATCAG 
TCATATACAT CTTTTTCTGA ATTTCAGAAA GTGACTGCCT TTCTAGTTAA TGTTTCAGAC 
AGTTTGATTT CAGGAAGTGA CTCAGAAACT GAGAGTTCAC AGAACACAGA TGTCAATGAA 
TGGCAGGAGT TCACTGACCA ACAACCATTG AATGAGCTTT CTAGTCCTTT GCCTAATCCA 
TCAGTAACTC CAAGGAAAGG AGATGAATTG ATTTTGGCAG TTAATAGTGA AGAGAAACAG 
CCAGATAGAG ATTTCCCTTT TGAAAACTGC CAAGACTTAC AGACACAAGG GAATAATAAT 
ATATCATCTA ATGACTCTCC TTCTGAAACA AATAATGTAA GTTTGTCAGG CAGCAGGAAA 
CACAAGCTAA GCCTTTCGTG GACAGAGTTA CCATTGTCTC AAGATTCATC CTCTGGAAAG 
CTGAGTCATA TTCTTTCAGA GGATACCTAT GTGGCACCAA GACAGGATAA AGAAAAGAGT 
TGCTCGGAGC ATAAAGTTTG TGTTGAACCC AAGCTCAGTT CCTTTTCATT CTTGGATGAC 
TTTTCAAAGA CAGATATAGA TTTGAAGCAG CAAGGTCCTA CAATTTCCCT GAATGAAGGA 
AAAGATCAGG AAGACCTTGA AATCAAAGAT GTTTTAGCGG ATGGAACAAA CAAAAAAGAA 
ACTGGATTTT CTCTCCCAGA AATTTATTAC ATGTCATTTC CATCCCCAAA TCTGAAAGCA 
AATTGCCAAT CACCTTCACT GCCTCTTAAA ATGTCACAGA GAAAACAAAA CTCGTTAGTC 
AAAAAAGCTA AAGCTGCATG TGAAGAAAAT CCAGAAGAAA GGCTTATTTC TAATAAAGAG 
TGTAAATATG CCATTCAGCA GTTGTCAATT GATTTCAACA GTATGCCTGC TATACCTGGC 
CCCCAGATCT TTCATGATGG AAAAATTGTT CAGTCAGTAT ATGAGCACAA GGCAGATAAA 
TCTGATATTC ATCACAGGAC CTCAGAGGGC CACGAGGTGG TGATATCTCC TTCTGGATCA 
CTTAGTGGGC TGATTCCTGA GGCTGAGCAT TGCCCAGCTA TCAGGGTTGA TGGTTCCACA 
CATACTGAAA GAGATGGTCT ACCTAGTGTC CAAGATAGCA TCTTGTCTAA AATACTATCC 
CCTGTCGACG AAGTGCTTTC TTATGGAAGT GCTGGCCTTC CATCCCCAAC CCAGAAGGTT 
TCATCTTTCG CCAGTGAAAA TCTCCCTTCT CCTCCTCCTG ACTTTGTGCT TTGGGCAAAT 
GTCAGTGATA TTGACTTCAA TTCAGAAGAC TTCCCTTCTC TGCCAGAAGA AATGGTATTT 
CCAGAAGATT CCAAGGATGT ACAAGATGAA GATTCATCTA TTCAAACTGG AGAGTTATCT 
TCTTTATCTG ATGAGATACT GCTGGAGGAA TTTTCTCCAA TACCTTTAGA TGTAGGGAAT 
TCCTGCTCAG TTCTGGATGA ACACTCCCTA GGATGGGACA AAGAGAGAGA AAGAAGTTTG 
GAAAGGAAGG AGGAATTGTT GAGAGTCGAA CCACAGCAGG ATATCAAAAG GAAGCAAAAA 
GTGGGCTGCT GGTACAGTCC TGCTTTGTCC AGAAGTGCCA GTAAAAGTCC AGCATCATTC 
TTCAAACTAT CCACAGATAA AGATGGCAAG AATCCACTGG CATTGGAGGA TGCAGAGAAC 
AGAGAACATA CACAACCAGA AACTCTGAAA TTCAGAGAGA CAAGCCATTT TGAAGGAAAA 
CACTGGACTA GTTTGGTATC CGATGAAGCA AAGAATGATC ATCACAAAGA AGACTTGGAA 
AGAGTAAAAC ATTCAGAGTA TGACATGGCT TATGATGAGT TATTTAGATC AGATAAAATG 
TCATACTTTT TGGCAGACGA CATAGAAAAG CCAAGTGACC ATAATTACCA AGATTCAGTT 
CATGATGAAT CATCCCTAAG GAGTAACAAG TGCTATGATG AGGTCAGAGT GGAATACCGG 
TTTTTAGAGA GAAAGCCAGA AATAAAGTTT AACAATAGAG ATAGCTCAAT GACAACAGTA 
GCCGCTTATC CTGAAACCCT GATGTTCCAA CCTACAGAGA TGGAGTCCTA CTTTATTCCT 
ACTCAGTCTG AGTCTGAAAG ATTTTTAAAT AAAGATTTCT CAGGCTGCCT AAATAGCCAA 
AAAGAACAGC CATATATGGC ACCTATAAAA AATGAGCCTG AGAAGTTACT TGAAGATGTT 
CATCCAATGG AAGAGAAAGA AATCAACTTA AAAAATAGTG TAATGGGCTC CCAACTTGAT 
CCTAGGCAAA AAACATATGG AATTGTAGAT TCTGTATCCA CTGAACTGAC CAGAAAAATC 
CTGTGGGATG CCTTAGCTGT GTTTGCAGAA CTTGCCAGAC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100932056 ( XM_031954951.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031810811.1 Sarcophilus harrisii coiled-coil domain containing 187 (CCDC187), transcript variant X2, mRNA.

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CloneID	OSa226576
Clone ID Related Accession (Same CDS sequence)	XM_031954951.1
Accession Version	XM_031954951.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	coiled-coil domain-containing protein 187 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTTG ACTACATGAC CTCCAATGTT CCTCTCAGTT CTTTGAGCTC CTCCTCTCCA 
TCTGAACAGA TCCAATCAAG GCAGAAAAAC TCTGTCTACC TGAGCCCTCA GTTACAGCGG 
ATGGAGAGTG AAATGGACAC CCCCAGCTGG CACAGCATCA GCAGCCAGAG CTGCGGATCA 
CTTGATTTTG GGCAAGAGAA GCTGTTTTCT TGGGAAAACT GGCACAATGC TAGTCCTGCC 
TATGCCAAAC TGAGAATGCC TCATTTTTCT GAGGACTACA ACATGGCAGC TGCATCAGTG 
TCACCCTGGG TCTTTCGGGA GTCAGTTATC TTCAGAGAAG AACCAGATGG CAAGACTTAC 
GGTGGACGAG TAACTCATTT GAATCCTGTG ATTTTTGGCC CAGTGTGGAA AGAGCAGGAC 
TTTAACTACC CACCTGAACC AGAGAGAATG TGTACAACTA GTGACTGGCC CTCCAAAATG 
CCCGTCTTTA TTCCCGTTCA GTCGGGAAGC CATCATCAGT TCTTGAATCA CCCAAGTGAC 
CCCTGGAGGG AAAAGTCACC TCAGCCAGTT GAGTCTCCCA CTAATTTTCA GAGATGGCCA 
TTGACAGGGG TAAAGAGAGC TTTCCCTGAA TCCCAGCTGG AGCAGCTGAG GGAGAAGATC 
CGGGCACAGA AACATCGACA TGCTAATGTT TTCCCTACCT CCTTCCAAGC TCGCTCTGGG 
ACCCCCCAAA CTCTGCCCTG GAAGCACGTG CCAAAGAGAA GCGTCTCCAA AATGAAGTTA 
GCTGCTTCAA AGCCAGCCCA CCCGGGCCAA GTCTCGGCTT CCAGCCCAGA TCCCTCTGAC 
ACTCACCATG CCGTTTCCCA CAAATTTATA CCAAAGAGAA AGAGCTACAG AGTTAGATTT 
GCATTATCAA CGCCAATAGA CGCACATCAA AGGGATAAAA ATAAAACTAC AGTGACCAAA 
AGCTGCCAGG TATCTTCTCA GTCACTTTCT CTTGGAAAAC AGCTAAAAGG CCAAGATGCT 
AAGGTGACTG GCGTTTCTGC ATGGAGAAAG GGACAGAGGC TTGCGAGGCT CCTCCTTGGG 
CCCCCTCCCA CGTTTCTCCA GCTCCAGAAA AGGGCTCTCT CCGGAGGCCT GGCCGCCACA 
AAGGAGTCAG GACTGACAGA GAAGACTCAA AAATCTGAGC ACATACCACC CTCCACCCGA 
GATATAAACA ACCAGAAAGG AGCAGCTCCT CTCCGTGGGA ACTCCCAGAA CTCTCCAGAA 
AGTTCAGCAC AAGTAGGCTG TGGTGGTTCT GAATCATTCC ATAGGATCTT AAACTTTTTG 
AAAAATCTCC AATCACGTGT TCAAGACAAG GCAGATCACA AAAGAACACG GCCCCAGTCT 
CCAAAGATCG CGTCTACCAA GAAAGCAGGC AAGTCAGAAT GTCATTCAAA ATCTCAAAAT 
GCAGCAGTGA GAAGGAATCT TCAATTTGTC TCAGGAGGAA AGAACCTCTC TTCAAGGATG 
GGTAGTTCTG GGACATCAAA AGCACCTGGA AAAGAAAATG CCTGCCAACG GACTCTCAAA 
AAAACAAATA AGGGTGTCCC TCGTCCCTGT GTCACTGCAG AAATGCAGGA ATTGATGAAT 
CAAAAAGTAT CTGAAAGAAA GAAAAGATGC CTTGAAGCCA AAATATATGT AAAGAAAGCC 
TTGGAGTCAA GAGATAAGTT GCAGAATGTA AAAGAGAAAG AAATTCCATT CAAGAAGTTG 
AATGCCAAAG TCTTACAAAT GTCATCCAAG AATATTGGTT CTGAGCAACA ATCCAAAGCC 
CAGGAAGCCA CGGAAAACAA AAGGGAGGCA GCTCTAGAGC ATGAAAAGCA GATGTTGGGC 
ACTGTGTTAA ATAATAAAGA GCTTCAAGAC AGAACTTCCC ATGATATTAA AACTCCAGTT 
ACAGTTGGGG CTTCTAAAAG TGGGGTTAAC CCTGCTCTGC CGGTCTTCAA AAGTATATCC 
CCTAGACCCT TGAAACAGCA AGGCTTGGCC CCTCCTAGAT CTTTACCTCC AGCTTTGACT 
CTTCAGTCAC ATCATCTTGA AAACTTGAGG AAGACTCAGA GCTCTATTGT ACCTAACAGA 
GAATCAAACT CTGCACCCTA CCAACACAAT CAGGAGAGGA TCCGGATTCT CGAGGCCACT 
GCTCAGATGC TGAAAGAGAG AATTGAATTT CTGACTGAGA AGTTAAACCT CTCAGGAGTA 
TGTCTGACAC TTAATGGCAA ATCTGAGAAG TGCATGGATG TACAAAGCAC CAATATCTCA 
TCAGTTCAGG AGTGCCCCAC AATGATAGTA TCATCTTGTT CTCTTGTACT TGGGCAGTCT 
TCCAGCCCAA CTGCCTTGGG AACCCCTGGA GGTAATGAAC CAGAAGTGAA CAAGCCCCTC 
AGTGACAGAG AGAAAGAGAT GACTCAGAAA GGTGGAAAAG ATGGAGTGGA AATGGAAACA 
AAGGTCCCAG CTGTTCTCAG CCCTGCTGCA ACTGCTGAAA TGTTATCCTG TAGAAGCCCA 
CGATTGAATA AGCAACCAAG TCCTTGCCTT GAGGAAGTAA TGAAACCTGT ACCTGATGAT 
TTTACTCTGA CTGAGGATTC TGAAATGGAA GAGTGGGCAC CAGAAGATAA ATTCACCTGT 
ACTCCTACTT TCTTCACAAG AAGTTCACAG AAAGAATTTG AAAAGCAAGA TAGCTTCATT 
GATCCTTCTG GAATTCAGAC GGAAGAAGAA CTGTCTGAAT TTTCTCTTGG AATCTCTGAA 
TCCTATAATG ACATCAAGAT ACGAGACTTT CCCACCAGAA GAAATGAGTT TTCAAGCTCT 
TCAAGCAAAC ATAGGGAGGA ATATGAAAGT TGCAACAAAT ATTCCAAAGA TGTCTGCATG 
GATTATTTAA GCAGCATGCA ACAGAAATCT CTTAACTTTC TTCAGAAAAT GAAGTTACAT 
CAGATAAAAC AGGAAAGAGA GTTAGAAATT CTGAAGCATA GAGCAGAACT GGAAGCTGCA 
GAAACTCAGA AATCTTTGGA TGAATTGGTC TTTAGGAATC ATTTGAAGGT GAGTCCTAAG 
CAGTCTTTGC ACGGCAGCCA CATCCATAGA ACTGAACTAG ACAATAAAGC AGAGAAATGG 
AAGATGCCAA AGACCCATGG AGGCCTGGAA TCCACAGACT GTCTAACTGG GCTGTCATCT 
ATGGGCAGGT CATATTCCAG CAGAACCAGT AGTCCAACAG TTTCCACCTG GGAAAGTGAG 
CCAAGGAGAA ATTTATTGGG TTGCACAGAT GCTGATAAGG AAAGAGGGAA GAAGCAAGAC 
TATACCACAT CATCACACTC CACTACATGG TTCTCATCAG CTTTTGGTTA TCCAAGTAGC 
AAGGTATTCT CTAAAGAAGA CTCCATGGAC AGCAGCAGCC AGAGGAATGA AATCTATCGG 
TCTAGCCTCT TCGACCGCTT TAGCCTGAGG ATGACTGATC AGTATTTAAA GGAAGAAGAG 
CTGAGAGCAC AGTACCAGAT AGCCCTCCTC AAGCTCCGGG AGAAAGCCCT AGAGGAGAAA 
GTTATAGCTG AATTGTCATG GCTAGAGCAA CAGAAAGCGT GTTTGGGGAA CAAAAGAGAT 
CACAGCTTAA TGACTGAGGA ACAGCACCAA ATACTAATAG ATCTGAAGAA AGAACAGGCG 
GAACTTAAAC ACCTACAGAA CATTTATGAA ATGGCTCATC AGGAGAGAAA ATTTTTGTTG 
ACACAACAAA AGGACCTCTC AACAATACAG CAGTCAACAA GTGATCTTCA GTCGGAATTT 
ACTGTCCTAG CCAGCAGACA CCAGTTTTCT AGTCCCTGGT CAGAAGCTAT AGAAAGCCAG 
AAGTCTGAAG ATGAGAGAAT CTTCAGATTT GAGAGGTTGG TTAGCAGTCC AGAGGGTTTG 
TTGTCTTCAA CATCATCTCA TACAGAAAGA TCCTCTTCAG TCTTGCCTGA CCTTGGGGAA 
AACCTGAAAT GCTATGAGAA CACACACCTT CTTGCTGAGA GGGAGATCCA GGGGAAACCT 
GATGAGGACT CATGCCTACA GCCACAGTGG CTGAAGGGGG GAAAGGACAG ATCAATGGTC 
AGTAACATAC CTGACCACAG GGAGCAAACT CTGGGCCAGT TCGTGAAGAA TGACAACCAG 
GAAAATAAAG AACCTGCTGA ACAGCACTGT ACTAGCTGGG CATGTCGACA GCAAGAAAGC 
TCTTTGGAAA TCAAACAAGA TCCGTATCAG AAGCCAACTA CAAGGACAGA GGAATATAAT 
TCAAAGACTG ACTTCAAGCC TAATATGGTG AAAAAAAGAC CTCAAAGTGA AGTAATAGGC 
TCATTAGAAA ATGGTGAGAG ATACATGCAA GGACAAAAAA ATGAACTAAA TTTTTTAAAA 
TTGGAAAGAA GTTTGGCTGG ACAAGAAGGT GGTCAATCTT TTCAGCAGGA AGACAGTGTT 
ATTTACAGTA GAAAAAATCA GGAGACCTCA GGACTGGACT CAGATGTCAT TAAGAGTCCA 
ATAGTTGAAC CAGAAGAGGA CAAAAGAAGG AAACAGAGGA TGAAGGGTAA ATCTCAAGAA 
GTTCCATTGA AAACTATCCA GATATGTCAC ACTTCTGCCA TTCAAGAATG CAAAAAAGAT 
CAAGAGAAGA AATCAGAGTA TACTCAATAT GATGAAAACC ACTTGCATAT TAAGTGTGAT 
ACGACTTCTG ACTCCTTAAA ATCAGGATCT GTTTTCAGTA CTTCTTTAGA ATCTCTTTCT 
TCAGACACAA TATCACCATG CAACTCACCT CTAGATTTAA AGAGTGATCA GTCATATACA 
TCTTTTTCTG AATTTCAGAA AGTGACTGCC TTTCTAGTTA ATGTTTCAGA CAGTTTGATT 
TCAGGAAGTG ACTCAGAAAC TGAGAGTTCA CAGAACACAG ATGTCAATGA ATGGCAGGAG 
TTCACTGACC AACAACCATT GAATGAGCTT TCTAGTCCTT TGCCTAATCC ATCAGTAACT 
CCAAGGAAAG GAGATGAATT GATTTTGGCA GTTAATAGTG AAGAGAAACA GCCAGATAGA 
GATTTCCCTT TTGAAAACTG CCAAGACTTA CAGACACAAG GGAATAATAA TATATCATCT 
AATGACTCTC CTTCTGAAAC AAATAATGTA AGTTTGTCAG GCAGCAGGAA ACACAAGCTA 
AGCCTTTCGT GGACAGAGTT ACCATTGTCT CAAGATTCAT CCTCTGGAAA GCTGAGTCAT 
ATTCTTTCAG AGGATACCTA TGTGGCACCA AGACAGGATA AAGAAAAGAG TTGCTCGGAG 
CATAAAGTTT GTGTTGAACC CAAGCTCAGT TCCTTTTCAT TCTTGGATGA CTTTTCAAAG 
ACAGATATAG ATTTGAAGCA GCAAGGTCCT ACAATTTCCC TGAATGAAGG AAAAGATCAG 
GAAGACCTTG AAATCAAAGA TGTTTTAGCG GATGGAACAA ACAAAAAAGA AACTGGATTT 
TCTCTCCCAG AAATTTATTA CATGTCATTT CCATCCCCAA ATCTGAAAGC AAATTGCCAA 
TCACCTTCAC TGCCTCTTAA AATGTCACAG AGAAAACAAA ACTCGTTAGT CAAAAAAGCT 
AAAGCTGCAT GTGAAGAAAA TCCAGAAGAA AGGCTTATTT CTAATAAAGA GTGTAAATAT 
GCCATTCAGC AGTTGTCAAT TGATTTCAAC AGTATGCCTG CTATACCTGG CCCCCAGATC 
TTTCATGATG GAAAAATTGT TCAGTCAGTA TATGAGCACA AGGCAGATAA ATCTGATATT 
CATCACAGGA CCTCAGAGGG CCACGAGGTG GTGATATCTC CTTCTGGATC ACTTAGTGGG 
CTGATTCCTG AGGCTGAGCA TTGCCCAGCT ATCAGGGTTG ATGGTTCCAC ACATACTGAA 
AGAGATGGTC TACCTAGTGT CCAAGATAGC ATCTTGTCTA AAATACTATC CCCTGTCGAC 
GAAGTGCTTT CTTATGGAAG TGCTGGCCTT CCATCCCCAA CCCAGAAGGT TTCATCTTTC 
GCCAGTGAAA ATCTCCCTTC TCCTCCTCCT GACTTTGTGC TTTGGGCAAA TGTCAGTGAT 
ATTGACTTCA ATTCAGAAGA CTTCCCTTCT CTGCCAGAAG AAATGGTATT TCCAGAAGAT 
TCCAAGGATG TACAAGATGA AGATTCATCT ATTCAAACTG GAGAGTTATC TTCTTTATCT 
GATGAGATAC TGCTGGAGGA ATTTTCTCCA ATACCTTTAG ATGTAGGGAA TTCCTGCTCA 
GTTCTGGATG AACACTCCCT AGGATGGGAC AAAGAGAGAG AAAGAAGTTT GGAAAGGAAG 
GAGGAATTGT TGAGAGTCGA ACCACAGCAG GATATCAAAA GGAAGCAAAA AGTGGGCTGC 
TGGTACAGTC CTGCTTTGTC CAGAAGTGCC AGTAAAAGTC CAGCATCATT CTTCAAACTA 
TCCACAGATA AAGATGGCAA GAATCCACTG GCATTGGAGG ATGCAGAGAA CAGAGAACAT 
ACACAACCAG AAACTCTGAA ATTCAGAGAG ACAAGCCATT TTGAAGGAAA ACACTGGACT 
AGTTTGGTAT CCGATGAAGC AAAGAATGAT CATCACAAAG AAGACTTGGA AAGAGTAAAA 
CATTCAGAGT ATGACATGGC TTATGATGAG TTATTTAGAT CAGATAAAAT GTCATACTTT 
TTGGCAGACG ACATAGAAAA GCCAAGTGAC CATAATTACC AAGATTCAGT TCATGATGAA 
TCATCCCTAA GGAGTAACAA GTGCTATGAT GAGGTCAGAG TGGAATACCG GTTTTTAGAG 
AGAAAGCCAG AAATAAAGTT TAACAATAGA GATAGCTCAA TGACAACAGT AGCCGCTTAT 
CCTGAAACCC TGATGTTCCA ACCTACAGAG ATGGAGTCCT ACTTTATTCC TACTCAGTCT 
GAGTCTGAAA GATTTTTAAA TAAAGATTTC TCAGGCTGCC TAAATAGCCA AAAAGAACAG 
CCATATATGG CACCTATAAA AAATGAGCCT GAGAAGTTAC TTGAAGATGT TCATCCAATG 
GAAGAGAAAG AAATCAACTT AAAAAATAGT GTAATGGGCT CCCAACTTGA TCCTAGGCAA 
AAAACATATG GAATTGTAGA TTCTGTATCC ACTGAACTGA CCAGAAAAAT CCTGTGGGAT 
GCCTTAGCTG TGTTTGCAGA ACTTGCCAGA CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031810811.1
CDS	310..7485
Translation	MVFDYMTSNVPLSSLSSSSPSEQIQSRQKNSVYLSPQLQRMESEMDTPSWHSISSQSCGSLDFGQEKLFSWENWHNASPAYAKLRMPHFSEDYNMAAASVSPWVFRESVIFREEPDGKTYGGRVTHLNPVIFGPVWKEQDFNYPPEPERMCTTSDWPSKMPVFIPVQSGSHHQFLNHPSDPWREKSPQPVESPTNFQRWPLTGVKRAFPESQLEQLREKIRAQKHRHANVFPTSFQARSGTPQTLPWKHVPKRSVSKMKLAASKPAHPGQVSASSPDPSDTHHAVSHKFIPKRKSYRVRFALSTPIDAHQRDKNKTTVTKSCQVSSQSLSLGKQLKGQDAKVTGVSAWRKGQRLARLLLGPPPTFLQLQKRALSGGLAATKESGLTEKTQKSEHIPPSTRDINNQKGAAPLRGNSQNSPESSAQVGCGGSESFHRILNFLKNLQSRVQDKADHKRTRPQSPKIASTKKAGKSECHSKSQNAAVRRNLQFVSGGKNLSSRMGSSGTSKAPGKENACQRTLKKTNKGVPRPCVTAEMQELMNQKVSERKKRCLEAKIYVKKALESRDKLQNVKEKEIPFKKLNAKVLQMSSKNIGSEQQSKAQEATENKREAALEHEKQMLGTVLNNKELQDRTSHDIKTPVTVGASKSGVNPALPVFKSISPRPLKQQGLAPPRSLPPALTLQSHHLENLRKTQSSIVPNRESNSAPYQHNQERIRILEATAQMLKERIEFLTEKLNLSGVCLTLNGKSEKCMDVQSTNISSVQECPTMIVSSCSLVLGQSSSPTALGTPGGNEPEVNKPLSDREKEMTQKGGKDGVEMETKVPAVLSPAATAEMLSCRSPRLNKQPSPCLEEVMKPVPDDFTLTEDSEMEEWAPEDKFTCTPTFFTRSSQKEFEKQDSFIDPSGIQTEEELSEFSLGISESYNDIKIRDFPTRRNEFSSSSSKHREEYESCNKYSKDVCMDYLSSMQQKSLNFLQKMKLHQIKQERELEILKHRAELEAAETQKSLDELVFRNHLKVSPKQSLHGSHIHRTELDNKAEKWKMPKTHGGLESTDCLTGLSSMGRSYSSRTSSPTVSTWESEPRRNLLGCTDADKERGKKQDYTTSSHSTTWFSSAFGYPSSKVFSKEDSMDSSSQRNEIYRSSLFDRFSLRMTDQYLKEEELRAQYQIALLKLREKALEEKVIAELSWLEQQKACLGNKRDHSLMTEEQHQILIDLKKEQAELKHLQNIYEMAHQERKFLLTQQKDLSTIQQSTSDLQSEFTVLASRHQFSSPWSEAIESQKSEDERIFRFERLVSSPEGLLSSTSSHTERSSSVLPDLGENLKCYENTHLLAEREIQGKPDEDSCLQPQWLKGGKDRSMVSNIPDHREQTLGQFVKNDNQENKEPAEQHCTSWACRQQESSLEIKQDPYQKPTTRTEEYNSKTDFKPNMVKKRPQSEVIGSLENGERYMQGQKNELNFLKLERSLAGQEGGQSFQQEDSVIYSRKNQETSGLDSDVIKSPIVEPEEDKRRKQRMKGKSQEVPLKTIQICHTSAIQECKKDQEKKSEYTQYDENHLHIKCDTTSDSLKSGSVFSTSLESLSSDTISPCNSPLDLKSDQSYTSFSEFQKVTAFLVNVSDSLISGSDSETESSQNTDVNEWQEFTDQQPLNELSSPLPNPSVTPRKGDELILAVNSEEKQPDRDFPFENCQDLQTQGNNNISSNDSPSETNNVSLSGSRKHKLSLSWTELPLSQDSSSGKLSHILSEDTYVAPRQDKEKSCSEHKVCVEPKLSSFSFLDDFSKTDIDLKQQGPTISLNEGKDQEDLEIKDVLADGTNKKETGFSLPEIYYMSFPSPNLKANCQSPSLPLKMSQRKQNSLVKKAKAACEENPEERLISNKECKYAIQQLSIDFNSMPAIPGPQIFHDGKIVQSVYEHKADKSDIHHRTSEGHEVVISPSGSLSGLIPEAEHCPAIRVDGSTHTERDGLPSVQDSILSKILSPVDEVLSYGSAGLPSPTQKVSSFASENLPSPPPDFVLWANVSDIDFNSEDFPSLPEEMVFPEDSKDVQDEDSSIQTGELSSLSDEILLEEFSPIPLDVGNSCSVLDEHSLGWDKERERSLERKEELLRVEPQQDIKRKQKVGCWYSPALSRSASKSPASFFKLSTDKDGKNPLALEDAENREHTQPETLKFRETSHFEGKHWTSLVSDEAKNDHHKEDLERVKHSEYDMAYDELFRSDKMSYFLADDIEKPSDHNYQDSVHDESSLRSNKCYDEVRVEYRFLERKPEIKFNNRDSSMTTVAAYPETLMFQPTEMESYFIPTQSESERFLNKDFSGCLNSQKEQPYMAPIKNEPEKLLEDVHPMEEKEINLKNSVMGSQLDPRQKTYGIVDSVSTELTRKILWDALAVFAELARQ

Target ORF information:

RefSeq Version	XM_031954951.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii coiled-coil domain containing 187 (CCDC187), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031954951.1

ORF Insert Sequence:

ATGGTATTTG ACTACATGAC CTCCAATGTT CCTCTCAGTT CTTTGAGCTC CTCCTCTCCA 
TCTGAACAGA TCCAATCAAG GCAGAAAAAC TCTGTCTACC TGAGCCCTCA GTTACAGCGG 
ATGGAGAGTG AAATGGACAC CCCCAGCTGG CACAGCATCA GCAGCCAGAG CTGCGGATCA 
CTTGATTTTG GGCAAGAGAA GCTGTTTTCT TGGGAAAACT GGCACAATGC TAGTCCTGCC 
TATGCCAAAC TGAGAATGCC TCATTTTTCT GAGGACTACA ACATGGCAGC TGCATCAGTG 
TCACCCTGGG TCTTTCGGGA GTCAGTTATC TTCAGAGAAG AACCAGATGG CAAGACTTAC 
GGTGGACGAG TAACTCATTT GAATCCTGTG ATTTTTGGCC CAGTGTGGAA AGAGCAGGAC 
TTTAACTACC CACCTGAACC AGAGAGAATG TGTACAACTA GTGACTGGCC CTCCAAAATG 
CCCGTCTTTA TTCCCGTTCA GTCGGGAAGC CATCATCAGT TCTTGAATCA CCCAAGTGAC 
CCCTGGAGGG AAAAGTCACC TCAGCCAGTT GAGTCTCCCA CTAATTTTCA GAGATGGCCA 
TTGACAGGGG TAAAGAGAGC TTTCCCTGAA TCCCAGCTGG AGCAGCTGAG GGAGAAGATC 
CGGGCACAGA AACATCGACA TGCTAATGTT TTCCCTACCT CCTTCCAAGC TCGCTCTGGG 
ACCCCCCAAA CTCTGCCCTG GAAGCACGTG CCAAAGAGAA GCGTCTCCAA AATGAAGTTA 
GCTGCTTCAA AGCCAGCCCA CCCGGGCCAA GTCTCGGCTT CCAGCCCAGA TCCCTCTGAC 
ACTCACCATG CCGTTTCCCA CAAATTTATA CCAAAGAGAA AGAGCTACAG AGTTAGATTT 
GCATTATCAA CGCCAATAGA CGCACATCAA AGGGATAAAA ATAAAACTAC AGTGACCAAA 
AGCTGCCAGG TATCTTCTCA GTCACTTTCT CTTGGAAAAC AGCTAAAAGG CCAAGATGCT 
AAGGTGACTG GCGTTTCTGC ATGGAGAAAG GGACAGAGGC TTGCGAGGCT CCTCCTTGGG 
CCCCCTCCCA CGTTTCTCCA GCTCCAGAAA AGGGCTCTCT CCGGAGGCCT GGCCGCCACA 
AAGGAGTCAG GACTGACAGA GAAGACTCAA AAATCTGAGC ACATACCACC CTCCACCCGA 
GATATAAACA ACCAGAAAGG AGCAGCTCCT CTCCGTGGGA ACTCCCAGAA CTCTCCAGAA 
AGTTCAGCAC AAGTAGGCTG TGGTGGTTCT GAATCATTCC ATAGGATCTT AAACTTTTTG 
AAAAATCTCC AATCACGTGT TCAAGACAAG GCAGATCACA AAAGAACACG GCCCCAGTCT 
CCAAAGATCG CGTCTACCAA GAAAGCAGGC AAGTCAGAAT GTCATTCAAA ATCTCAAAAT 
GCAGCAGTGA GAAGGAATCT TCAATTTGTC TCAGGAGGAA AGAACCTCTC TTCAAGGATG 
GGTAGTTCTG GGACATCAAA AGCACCTGGA AAAGAAAATG CCTGCCAACG GACTCTCAAA 
AAAACAAATA AGGGTGTCCC TCGTCCCTGT GTCACTGCAG AAATGCAGGA ATTGATGAAT 
CAAAAAGTAT CTGAAAGAAA GAAAAGATGC CTTGAAGCCA AAATATATGT AAAGAAAGCC 
TTGGAGTCAA GAGATAAGTT GCAGAATGTA AAAGAGAAAG AAATTCCATT CAAGAAGTTG 
AATGCCAAAG TCTTACAAAT GTCATCCAAG AATATTGGTT CTGAGCAACA ATCCAAAGCC 
CAGGAAGCCA CGGAAAACAA AAGGGAGGCA GCTCTAGAGC ATGAAAAGCA GATGTTGGGC 
ACTGTGTTAA ATAATAAAGA GCTTCAAGAC AGAACTTCCC ATGATATTAA AACTCCAGTT 
ACAGTTGGGG CTTCTAAAAG TGGGGTTAAC CCTGCTCTGC CGGTCTTCAA AAGTATATCC 
CCTAGACCCT TGAAACAGCA AGGCTTGGCC CCTCCTAGAT CTTTACCTCC AGCTTTGACT 
CTTCAGTCAC ATCATCTTGA AAACTTGAGG AAGACTCAGA GCTCTATTGT ACCTAACAGA 
GAATCAAACT CTGCACCCTA CCAACACAAT CAGGAGAGGA TCCGGATTCT CGAGGCCACT 
GCTCAGATGC TGAAAGAGAG AATTGAATTT CTGACTGAGA AGTTAAACCT CTCAGGAGTA 
TGTCTGACAC TTAATGGCAA ATCTGAGAAG TGCATGGATG TACAAAGCAC CAATATCTCA 
TCAGTTCAGG AGTGCCCCAC AATGATAGTA TCATCTTGTT CTCTTGTACT TGGGCAGTCT 
TCCAGCCCAA CTGCCTTGGG AACCCCTGGA GGTAATGAAC CAGAAGTGAA CAAGCCCCTC 
AGTGACAGAG AGAAAGAGAT GACTCAGAAA GGTGGAAAAG ATGGAGTGGA AATGGAAACA 
AAGGTCCCAG CTGTTCTCAG CCCTGCTGCA ACTGCTGAAA TGTTATCCTG TAGAAGCCCA 
CGATTGAATA AGCAACCAAG TCCTTGCCTT GAGGAAGTAA TGAAACCTGT ACCTGATGAT 
TTTACTCTGA CTGAGGATTC TGAAATGGAA GAGTGGGCAC CAGAAGATAA ATTCACCTGT 
ACTCCTACTT TCTTCACAAG AAGTTCACAG AAAGAATTTG AAAAGCAAGA TAGCTTCATT 
GATCCTTCTG GAATTCAGAC GGAAGAAGAA CTGTCTGAAT TTTCTCTTGG AATCTCTGAA 
TCCTATAATG ACATCAAGAT ACGAGACTTT CCCACCAGAA GAAATGAGTT TTCAAGCTCT 
TCAAGCAAAC ATAGGGAGGA ATATGAAAGT TGCAACAAAT ATTCCAAAGA TGTCTGCATG 
GATTATTTAA GCAGCATGCA ACAGAAATCT CTTAACTTTC TTCAGAAAAT GAAGTTACAT 
CAGATAAAAC AGGAAAGAGA GTTAGAAATT CTGAAGCATA GAGCAGAACT GGAAGCTGCA 
GAAACTCAGA AATCTTTGGA TGAATTGGTC TTTAGGAATC ATTTGAAGGT GAGTCCTAAG 
CAGTCTTTGC ACGGCAGCCA CATCCATAGA ACTGAACTAG ACAATAAAGC AGAGAAATGG 
AAGATGCCAA AGACCCATGG AGGCCTGGAA TCCACAGACT GTCTAACTGG GCTGTCATCT 
ATGGGCAGGT CATATTCCAG CAGAACCAGT AGTCCAACAG TTTCCACCTG GGAAAGTGAG 
CCAAGGAGAA ATTTATTGGG TTGCACAGAT GCTGATAAGG AAAGAGGGAA GAAGCAAGAC 
TATACCACAT CATCACACTC CACTACATGG TTCTCATCAG CTTTTGGTTA TCCAAGTAGC 
AAGGTATTCT CTAAAGAAGA CTCCATGGAC AGCAGCAGCC AGAGGAATGA AATCTATCGG 
TCTAGCCTCT TCGACCGCTT TAGCCTGAGG ATGACTGATC AGTATTTAAA GGAAGAAGAG 
CTGAGAGCAC AGTACCAGAT AGCCCTCCTC AAGCTCCGGG AGAAAGCCCT AGAGGAGAAA 
GTTATAGCTG AATTGTCATG GCTAGAGCAA CAGAAAGCGT GTTTGGGGAA CAAAAGAGAT 
CACAGCTTAA TGACTGAGGA ACAGCACCAA ATACTAATAG ATCTGAAGAA AGAACAGGCG 
GAACTTAAAC ACCTACAGAA CATTTATGAA ATGGCTCATC AGGAGAGAAA ATTTTTGTTG 
ACACAACAAA AGGACCTCTC AACAATACAG CAGTCAACAA GTGATCTTCA GTCGGAATTT 
ACTGTCCTAG CCAGCAGACA CCAGTTTTCT AGTCCCTGGT CAGAAGCTAT AGAAAGCCAG 
AAGTCTGAAG ATGAGAGAAT CTTCAGATTT GAGAGGTTGG TTAGCAGTCC AGAGGGTTTG 
TTGTCTTCAA CATCATCTCA TACAGAAAGA TCCTCTTCAG TCTTGCCTGA CCTTGGGGAA 
AACCTGAAAT GCTATGAGAA CACACACCTT CTTGCTGAGA GGGAGATCCA GGGGAAACCT 
GATGAGGACT CATGCCTACA GCCACAGTGG CTGAAGGGGG GAAAGGACAG ATCAATGGTC 
AGTAACATAC CTGACCACAG GGAGCAAACT CTGGGCCAGT TCGTGAAGAA TGACAACCAG 
GAAAATAAAG AACCTGCTGA ACAGCACTGT ACTAGCTGGG CATGTCGACA GCAAGAAAGC 
TCTTTGGAAA TCAAACAAGA TCCGTATCAG AAGCCAACTA CAAGGACAGA GGAATATAAT 
TCAAAGACTG ACTTCAAGCC TAATATGGTG AAAAAAAGAC CTCAAAGTGA AGTAATAGGC 
TCATTAGAAA ATGGTGAGAG ATACATGCAA GGACAAAAAA ATGAACTAAA TTTTTTAAAA 
TTGGAAAGAA GTTTGGCTGG ACAAGAAGGT GGTCAATCTT TTCAGCAGGA AGACAGTGTT 
ATTTACAGTA GAAAAAATCA GGAGACCTCA GGACTGGACT CAGATGTCAT TAAGAGTCCA 
ATAGTTGAAC CAGAAGAGGA CAAAAGAAGG AAACAGAGGA TGAAGGGTAA ATCTCAAGAA 
GTTCCATTGA AAACTATCCA GATATGTCAC ACTTCTGCCA TTCAAGAATG CAAAAAAGAT 
CAAGAGAAGA AATCAGAGTA TACTCAATAT GATGAAAACC ACTTGCATAT TAAGTGTGAT 
ACGACTTCTG ACTCCTTAAA ATCAGGATCT GTTTTCAGTA CTTCTTTAGA ATCTCTTTCT 
TCAGACACAA TATCACCATG CAACTCACCT CTAGATTTAA AGAGTGATCA GTCATATACA 
TCTTTTTCTG AATTTCAGAA AGTGACTGCC TTTCTAGTTA ATGTTTCAGA CAGTTTGATT 
TCAGGAAGTG ACTCAGAAAC TGAGAGTTCA CAGAACACAG ATGTCAATGA ATGGCAGGAG 
TTCACTGACC AACAACCATT GAATGAGCTT TCTAGTCCTT TGCCTAATCC ATCAGTAACT 
CCAAGGAAAG GAGATGAATT GATTTTGGCA GTTAATAGTG AAGAGAAACA GCCAGATAGA 
GATTTCCCTT TTGAAAACTG CCAAGACTTA CAGACACAAG GGAATAATAA TATATCATCT 
AATGACTCTC CTTCTGAAAC AAATAATGTA AGTTTGTCAG GCAGCAGGAA ACACAAGCTA 
AGCCTTTCGT GGACAGAGTT ACCATTGTCT CAAGATTCAT CCTCTGGAAA GCTGAGTCAT 
ATTCTTTCAG AGGATACCTA TGTGGCACCA AGACAGGATA AAGAAAAGAG TTGCTCGGAG 
CATAAAGTTT GTGTTGAACC CAAGCTCAGT TCCTTTTCAT TCTTGGATGA CTTTTCAAAG 
ACAGATATAG ATTTGAAGCA GCAAGGTCCT ACAATTTCCC TGAATGAAGG AAAAGATCAG 
GAAGACCTTG AAATCAAAGA TGTTTTAGCG GATGGAACAA ACAAAAAAGA AACTGGATTT 
TCTCTCCCAG AAATTTATTA CATGTCATTT CCATCCCCAA ATCTGAAAGC AAATTGCCAA 
TCACCTTCAC TGCCTCTTAA AATGTCACAG AGAAAACAAA ACTCGTTAGT CAAAAAAGCT 
AAAGCTGCAT GTGAAGAAAA TCCAGAAGAA AGGCTTATTT CTAATAAAGA GTGTAAATAT 
GCCATTCAGC AGTTGTCAAT TGATTTCAAC AGTATGCCTG CTATACCTGG CCCCCAGATC 
TTTCATGATG GAAAAATTGT TCAGTCAGTA TATGAGCACA AGGCAGATAA ATCTGATATT 
CATCACAGGA CCTCAGAGGG CCACGAGGTG GTGATATCTC CTTCTGGATC ACTTAGTGGG 
CTGATTCCTG AGGCTGAGCA TTGCCCAGCT ATCAGGGTTG ATGGTTCCAC ACATACTGAA 
AGAGATGGTC TACCTAGTGT CCAAGATAGC ATCTTGTCTA AAATACTATC CCCTGTCGAC 
GAAGTGCTTT CTTATGGAAG TGCTGGCCTT CCATCCCCAA CCCAGAAGGT TTCATCTTTC 
GCCAGTGAAA ATCTCCCTTC TCCTCCTCCT GACTTTGTGC TTTGGGCAAA TGTCAGTGAT 
ATTGACTTCA ATTCAGAAGA CTTCCCTTCT CTGCCAGAAG AAATGGTATT TCCAGAAGAT 
TCCAAGGATG TACAAGATGA AGATTCATCT ATTCAAACTG GAGAGTTATC TTCTTTATCT 
GATGAGATAC TGCTGGAGGA ATTTTCTCCA ATACCTTTAG ATGTAGGGAA TTCCTGCTCA 
GTTCTGGATG AACACTCCCT AGGATGGGAC AAAGAGAGAG AAAGAAGTTT GGAAAGGAAG 
GAGGAATTGT TGAGAGTCGA ACCACAGCAG GATATCAAAA GGAAGCAAAA AGTGGGCTGC 
TGGTACAGTC CTGCTTTGTC CAGAAGTGCC AGTAAAAGTC CAGCATCATT CTTCAAACTA 
TCCACAGATA AAGATGGCAA GAATCCACTG GCATTGGAGG ATGCAGAGAA CAGAGAACAT 
ACACAACCAG AAACTCTGAA ATTCAGAGAG ACAAGCCATT TTGAAGGAAA ACACTGGACT 
AGTTTGGTAT CCGATGAAGC AAAGAATGAT CATCACAAAG AAGACTTGGA AAGAGTAAAA 
CATTCAGAGT ATGACATGGC TTATGATGAG TTATTTAGAT CAGATAAAAT GTCATACTTT 
TTGGCAGACG ACATAGAAAA GCCAAGTGAC CATAATTACC AAGATTCAGT TCATGATGAA 
TCATCCCTAA GGAGTAACAA GTGCTATGAT GAGGTCAGAG TGGAATACCG GTTTTTAGAG 
AGAAAGCCAG AAATAAAGTT TAACAATAGA GATAGCTCAA TGACAACAGT AGCCGCTTAT 
CCTGAAACCC TGATGTTCCA ACCTACAGAG ATGGAGTCCT ACTTTATTCC TACTCAGTCT 
GAGTCTGAAA GATTTTTAAA TAAAGATTTC TCAGGCTGCC TAAATAGCCA AAAAGAACAG 
CCATATATGG CACCTATAAA AAATGAGCCT GAGAAGTTAC TTGAAGATGT TCATCCAATG 
GAAGAGAAAG AAATCAACTT AAAAAATAGT GTAATGGGCT CCCAACTTGA TCCTAGGCAA 
AAAACATATG GAATTGTAGA TTCTGTATCC ACTGAACTGA CCAGAAAAAT CCTGTGGGAT 
GCCTTAGCTG TGTTTGCAGA ACTTGCCAGA CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100932056 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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