ASH1L cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASH1L
Entrez Gene ID	100931585
Full Name	ASH1 like histone lysine methyltransferase
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023502250.1	XP_023358018.1	histone-lysine N-methyltransferase ASH1L isoform X1
XM_031966722.1	XP_031822582.1	histone-lysine N-methyltransferase ASH1L isoform X1
XM_031966719.1	XP_031822579.1	histone-lysine N-methyltransferase ASH1L isoform X1
XM_031966721.1	XP_031822581.1	histone-lysine N-methyltransferase ASH1L isoform X1
XM_003768076.3	XP_003768124.1	histone-lysine N-methyltransferase ASH1L isoform X2
XM_031966723.1	XP_031822583.1	histone-lysine N-methyltransferase ASH1L isoform X2
XM_003768076.2	XP_003768124.1	histone-lysine N-methyltransferase ASH1L
XM_031966720.1	XP_031822580.1	histone-lysine N-methyltransferase ASH1L isoform X1

Pathways from BioSystems
Gene Ontology

The following ASH1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASH1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa130098	XM_003768076.3 Latest version!	Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa130098	XM_031966723.1 Latest version!	Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa154248	XM_031966721.1 Latest version!	Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa154248	XM_031966720.1 Latest version!	Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa154248	XM_031966722.1 Latest version!	Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa154248	XM_023502250.1 Latest version!	Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa154248	XM_031966719.1 Latest version!	Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OSa130098	XM_003768076.2	Sarcophilus harrisii ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASH1L ( XM_003768076.3 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003768124.1 Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa130098
Clone ID Related Accession (Same CDS sequence)	XM_003768076.3 , XM_031966723.1 , XM_003768076.2
Accession Version	XM_003768076.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	histone-lysine N-methyltransferase ASH1L isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838832.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_003768076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATA GGAATACTGC TATGTTAGGA GTGGGTACCG ACTCCGAAGG ATTTTCAAGG 
AAGAGTCCTT CTGCTGTCAG CACTGGAACA CTGGTTAGTA AGAGAGAAGT GGAACTAGAG 
AATAGCACTA AGGAAGAAGA GGATCTTTGG AGGCGGAATA GGGACAGAAA CATTGAGACA 
GGGAAAAATG ATGGTTTGAC TGATGCACAG CAGTTTTCTG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAGTTGAA AATTGGCCTT CAGGCTAAGA GAACTAAAAA GCCTCCTAAA 
AACTTGGAGA ACTATGTATG TCGACCTGCT ATAAAAACAA GCATTAAGCA TCCAAGGAAA 
GCATTGAAAA GTGGGAAGAT GACAGATGAA AAAAATGAAC TTTGCCCTTC AAAACGAGAC 
CCTTCAAAAC TTTACAAGAA AGCAAATGAT GTTGCAGCTG TTGAATGCCG CTCTGAAGAA 
GGCATCCACC TTCATTCACA GGGAGAAAAT AATCCTGTGT CAAAGAAGCT TTCTCCACTA 
CACTCTGAAA TGACAGATTA TGTTAAATCA GCACCATCTA CTCTTGTTGG TAACCGTGAT 
CCTGACTCGA AGGACAGAGC TCTACTTAAT GGAGCAACAA GTGTAACAGA AAAATTGGCA 
CAGCTCATTG CTACTTGTCC ACCCTCCAAG TCTTCCAAGA CAAAGCCAAA AAAGTTAGGG 
GCTGGTATTA CAGCAGGATT GGTTAGCAAA GACTTGATCA GAAAGCCAGG CATTGGTCCT 
ATGGCAGGAA TTATTCACAA GGACTTGATT AAGAAACCAA CAATTAGCCC AGCAGTTGGG 
TTGGTAACTA AAGACCCTGG GAAAAAGCCA ATCTTTAGTG CTGCAGTAGG ATTGGTCAGT 
AAAGACTCTG GGAAAAAATT AGGAACTGGG ACTACTGCGG TATTCATAAA CAAGGACTTA 
GGAAAGAAAC CAGGGACTAT CACTACAGTG GGACTGGTGA GCAGAGAGTC AGGGAAGAAA 
TTAGGAATTG GTATCGTCCC AGGTTTGGTG AGTAAAGAAT CAGGAAAGAA GTTGGGACTT 
GGTACTGTAG TTGGGCTTGT TAATAAAGAT TCAGTAAAGA AGTTTGGCCC TGCCATTGGA 
CTGGTTGCAA AGGACTGTGG GAAGAAGACT ATAACAAATT CATCAGTGGG ATTGGTTAAT 
AAGGACCCTG CAAAGAAATT GGTGGCTTGC CCTGTAGCAG GATTGGTCAA CAAAGACATT 
GTGAATTTAA AAGCTGAAGC CTTGCTCCCT GTTCAAGAAC CACTTAAGGT TTCTTGCAAT 
ACAAATACTA GTAGTCATGA AAGTCAGGAG CATTCTGAAT CCCTGAAAGA TAGTGCCACC 
AGCAAAGCTT TTGAGAAGAC TATTATTCGT CAGAATAAAG ATAGTTTATT AGAAAAGTTC 
TCAATTCGAA AAGAAATCAT TACTTTGGAC AAAGACATTT TTAATGAAGG AACTTGCATA 
CAGCAGGACA GTTTCTTATC CAGTGAAAGG GGGACCTATG TTGAAACTAC AAAGCATGAG 
AAGCAGCCTC CAGTGTATTG CACTTCACCA GATTTTCAAA TGGGAGGCAC TTCTGATGCA 
TCAACAGCTA AGTCTCCCTT TAGTGCAATA GGGGAGAGTA ATCTCCCTTC CCCTTCACCA 
GCCTTGTCTG TTACGCCCTT AAACAGAAGT CCCCCTGGAA CTTCTTCTCA GTTGGCTACT 
AATCCATTAC ATTTAGGTCC TTCCACAGAA CTCATAGAAG GAATTGCTGA ATCTATTGGA 
AAAACCCAAT TCAGTTCTGA AAATTCCCAT ATGAACCTTG GTCACAGGTC TCTGGTTCAC 
AGCATGAACA CTGATTGCAA AGGAAATGAT AAAGAGTTAA ATGATTCAAA AGCTTCTCAC 
ACAGATAACT CAAGAATGGG CTCCTCCATA GGAAAAAAGC CAAGTCTGGC TTCAGAATCC 
AGTATTCATG CTACAATTAC TCCCTCAGTT GTTAGTTTCA CTAGTTTATT TAGCAGTAAA 
CCCTTTCTGA AGATTGGTGC AGTAACTGCA TCAGAGAAAC ACTGCCAAGT TGCTGAAAGC 
CTGAGCACTA GTTTGCAGTC CAAACCATTA AAAAAAAGAA AAGGAAGGAA ACCACGATGG 
ACTAAAGTGG TAGCAAGAAA CACATGCCGG CCTTCAAAAG GGCTAGAATT AGAAAGGTCA 
GAGCTTTTTA AAAATGTTTC ATGTAGCTCT TTGTCAAATA GCAATTCCGA GCCAGCCAAG 
TTTATGAAAA ACATTGGGCC ATCTTCATTT GTGGAGCATG ACTTCCTTAA ATGCCGATTG 
CCAAAGCTGA GTAAATCTAG CACAGCTCCA TCTCTTGCTC TCTTAACTGA AACTGACAAG 
CAGTCTCATA AGTCCTTTGC CAGCCACAAA CTTTCCTCTA GTATATGCAT GTCTGGTGAC 
CTTTTACCTG ACATTTATAA ACCTAAAAGA GGAAGACCTA AAACTAAGGA GATGCCCCAA 
CTAGAAGGTC CACCCAAAAG GACCCTAAAA ATCCCGGCCT CTAAAGTTTT TTCATTACAG 
TCAAAAGAGG AGCAAGAACC CCCTATTTTG CAGCCAGAAA TTGAAATCCC TTCCTTCAAG 
CAGAGCCTAT CAGTATCACC CTTTCCCAAA AAAAGAGGAA GGCCAAAAAG ACAAATCAGA 
TCACCAGTCA AGATGAAACC ACCTGTGCTA TCAGTGGCAC CCTTTGTAGC CACTGAAAGT 
CCAAGTAAAC TAGAGTCTGA GAGTGATAAA CATAGAAGTA GTAGTGACTT CTTTGAAAGG 
AAAGATCAAC TTCAGGGTCC AGATGACAGT CATAAACCAA GTGTGTGTGG TATGAGTGAC 
CTTGAGATGG AACCTGATAA AAAAATTAAT AAGAGAAACA ATGGCCAATT AATGAAAACT 
ATCATTCGCA AAATAAATAA GATGAAGACT TTGAAGAGAA AGAAACTGCT GAATCAGATT 
CTCTCAAGTT CTGTTGAATC AAGTAACAAA GGGAAAGTGC AATCCAAACT CCACAATACA 
GTGTCAACAC TTGCTGCCAC TTTTGGCTCC AAACTGGGCC AACAGATAAA TGTCAGCAAG 
AAAGGAACCA TTTATATAGG AAAGAGGCGA GGCCGGAAAC CAAAAGCTGT GCTGAACGGC 
ATCTTAACTA GTAGCCCAGC TAGTCTGGCT GTCCTAGAGA AAACAGCTCA TCAGGTAGCT 
GGGTCAGCAT TAGGACAGAT TCTCCCCCCA CTCTTGCCTT CAGCAGCAAG TAGTTCTGAG 
GTTCTTCCAT CCCCCATCTG TTCTCAGTCT TCTGGGACTA GTGGGGGCCA GAGTCCTGTC 
AGCAGTGATG CAGGATTTGT TGAGCCCAGT TCAGTGCCAT ATTTACATAT ACACTCCAGA 
CAGGGCAGTA TGATTCAGAC ACTTGCTATG AAGAAGGCTT CAAAGGGAAG GAGACGGTTG 
TCTCCTCCTA CTTTGTTGCC AAATTCTCCT TCTCACTTGA GTGAACTCAC TTCTCTGAAG 
GAAGCTACTC CTTCCCCAAT TAGTGAATCT CATAGTGATG AGACCATTCC TAGTGATAGT 
GGAATTGGAA CAGATAATAA TAGCACATCA GACAGGGCAG AAAAGTTTTG TGGGCAGAAA 
AAGAGGAGGC ATTCTTTTGA ACATGTTTCT CTCATTCCCC CTGAAACCTC CACAGTCCTA 
AGTAGTCTCA AAGAGAAACA TAAACACAAA TGTAAACGCA GGAGTCATGA TTACCTCAGT 
TATGACAAAA TGAAAAGACA GAAGCGAAAA CGGAAAAAGA AGTATCCCCA GTTACGAAAC 
AGACAGGATC CAGACTTTCT TGCTGAGCTG GAGGAATTAA TAAGTCGTTT AAGTGAAATT 
CGGATCACTC ATCGAAGTCA TCATTTTATT CCCCGTGATC TCCTACCCAC TATTTTTCGA 
ATTAACTTTA ACAGTTTCTA TACGCACCCC ACTTTTCCCT TAGACCCTTT GCACTACATT 
AGAAAACCTG ACTTAAAGAA AAAAAGAGGG AGGCCTCCCA AGATGAGGGA AGCAATGGCT 
GAAATGCCTT TTATGCATAG CCTTAGTTTC CCTCTCTCTA GTACTGGGTT TTACCCCTCT 
TATGGCATGC CTTATTCTCC CTCTCCACTT GCAGCTGCTC CCATAGGATT AGGATACTAT 
GGAAGGTACC CTCCAACTCT TTATCCACCA CCCCCCTCTC CTTCTTTCAC CACTCCTTTG 
CCACCTCCTT CTTACATGCA TGCTGGTCAT TTGCTTCTCA ATCCTGCCAA GTACCACAAG 
AAGAAACATA AGCTGCTTCG TCAGGAAGCC TTTCTTACAA CAAGTAGGAC ACCCCTTCTC 
TCCATGAGCA CCTACCCCAG TGTCCCTCCT GAGATGGCCT ATGGTTGGAT GGTGGAGCAT 
AAACACCGGC ACCGGCACAA ACACAGAGAA CACCGTTCTT CTGAGCAACC CCAGGTTTCC 
ATGGACACTT TAGCTACCAA TAGCTCTTCC AGAACTGTCC TAGAATCGTT GAAGCGCTAT 
CGATTTGGTA AAGATGCTGT TGGGGAGCGA TATAAACACA AAGAGAAGCA TCGTTGTCAC 
ATGTCCTGTC CTCATCTGTC CCCATCAAAA AGCTTATTAA GCCGAGAGGA GCAATGGGTC 
CACCGAGAGC CTTCAGAATC CACTTCATTG GCCTTGGGGT TGCAGACACC TTTACAGATT 
GACTGTTCAG AAACGTCTCC AGGTTTGTCC CTGGGAGGTT TTACACCTAG CTCTGAGCCA 
GCCAGCAGTG ATGAACACAC AAACCTTTTC ACAAGTGCAA TAGGCAGCTG CAGAGTATCA 
AACTCTACTA ATACAAGTGG CCGGAAGAAA TTGACTGACA GTCCAGGACT CTTTACCGCA 
CAGGACACTT CATTAAATCG GCCTCTTAGA AAGGAAACTT TGCCTTCCAA TGAAAGGGCA 
CTGCAAACTT TGACAGGCTC CCAGCCAGTC CTAGACAAGC CTTCCCAAAG GCCATCAGAG 
AGCACAAACT GTAGCCCTAC TCGAAAGAGA TCTTCATCAG AGAGCACTTC TTCAACAGTG 
ATTGGAATCC CTTCCCGAGG TACAAGATTA GGAACATCTG TGGATGACTC TGTGGATAGC 
CTGCTACAGC GTATGGCGCA ACATGAAAGC CAAGGGACCT TAGAGAAAAA CATTGATGCT 
GTCATTGCCA CAGTGTCCAT CCCACCTTCC TCAAGCCCAG GTCATACTCA TGGTAGGGAA 
CGAAGTCTGG GGAAATCAGA CAGTCTGCTA GTACCCACAG CACCTAGTGA CTCCTGCAGC 
AACACCACCA CCACCATCTC ACTCCTTTCT GAGAGGTTGC CAAGCAGCTG TTCCCCCCAT 
CATCTCAAAA GGAGTGTGGT AGAAGCCATG CAACGCCAAG CCCGGAAAAT GTGCAATTAT 
AACAAAATCT TGGCCACCAA GAAAAACCTA GACCATGTCA ATAAAATCTT AAAAGCCAAA 
AAGCTTCAAA GACAGGCAAG GACAGGTAAC AATTTTGTGA AGCGCAGACC AGGCCGACCC 
CGGAAATATC CCCTCCAGGC TGTGGTGTCA ATGCAGGCAT TCCAGGCTGC CCAATTTGCC 
AGCCCAGAAT TAGATGAAGG GGAGGAAGGC AGAGCACTAC ATCTTGGTCC TGATACAGTT 
ACAGATGTCA TTGAGGCTGT TGTTCAGAGT GTGAACCTGA ACCCAGAACT TAGAAAAGGA 
TGGAAAAGGA AAAGTTGGCT TCTGGAGGAA GAGGCAAGGA AAAGACAGAA GATACTTCCA 
GAGGAAGAAC AGGAAAATAA TAAAAGCTTT CCTGAGACAA CAATTGAATC TCCTAGTCCT 
CCAGAAGCCC CACCAAAGCC ACCTGAACCT GAAAATACCC TGCAGCCTTT GCCTTCTCTT 
ATCCCTCGGG AAAAGAAGGC CCCACGTCCC CCAAAGAAGA AATATCAGAA AGCAGGGCTC 
TATTCTGATG TATACAAAAC TACAGATCCA AAGAGCAGAC TGATACAATT AAAGAAAGAA 
AAACTGGAGT ATACTCCTGG AGAACATGAA TATGGATTAT TCCCAGCCCC TATTCATGTT 
GGAAAGTATT TGAGACAAAA GAGAATTGAC TTTCAGCTTC CTTATGACAT CCTCTGGCAA 
TGGAAACACA ATCAGCTATA TAAAAAGCCA GATGTCCCAC TATACAAAAA AATCCGTTCA 
AATGTCTACG TTGATGTCAA ACCTCTTTCT GGCTATGAGG CTACCACCTG CAACTGTAAA 
AAACCAGATG ATGGTGATAG GAAGGGTTGT ATGGATGACT GCCTCAACAG AATGATCTTT 
GCTGAGTGTT CCCCCAATAC CTGCCCCTGT GGAGAGCAGT GCTGCAATCA GAGGATACAA 
AGGCATGAGT GGGTACAGTG TCTTGAGCGG TTCCGAGCTG AGGAGAAGGG ATGGGGGATC 
CGGACCAAAG AACCTTTGAA AGCTGGCCAG TTCATTATTG AATACTTGGG AGAAGTTGTC 
AGTGAGCAGG AGTTCAGAAA CCGAATGATT GAACAGTACC ATAATCACAG TGACCATTAC 
TGCCTAAATC TGGACAGTGG AATGGTGATT GACAGCTACC GAATGGGCAA TGAGGCCCGG 
TTCATCAACC ACAGCTGTAA CCCAAATTGT GAAATGCAGA AATGGTCTGT TAATGGAGTC 
TACCGGATTG GGCTCTATGC CCTGAAAGAT ATGCCAGCTG GTACTGAACT AACCTATGAC 
TACAATTTTC ATTCTTTTAA TGTTGAGAAA CAGCAACTTT GTAAGTGTGG TTTTGACAAA 
TGCCGGGGTA TCATCGGAGG CAAGAGCCAA CGCATGAATG GACTCACCAG CAAAAGCAGC 
CAACCTGTGA CCACACACAG ACGGCCAGGA CGGTCAAAAG AGAAGAGAAA ATCCAAGCAC 
AAGTTGAAGA AAAGGAGAGG CCATCTTTCT GAGGAGCCTA ATGAAAATGT CAGTACCCCA 
ACCAGAATGA CCCCCCAACT GCAAATGAAG CCAATGTCCA ACCGTGAAAG GAACTTTGTG 
TTAAAACATC ATGTATTCCT GGTCCGGAAC TGGGAGAAAA TCAGACAGAA GCAAGAGGAG 
GTAAAACACA CCAGTGACAA TATCCATTCA GCTTCCTTAT ATACCCGCTG GAATGGTATC 
TGCCGAGATG ATGGAAACAT CAAATCTGAT GTCTTCATGA CCCAGTTCTC CGCCTTGCAG 
ACAGCCCGCT CAGTTCGTAC AAGGAGATTG GCAGCTGCAG AGGAGAACAT TGAAGTGGCC 
CGGGCAGCCC GCCTGGCCCA GATATTCAAA GAGATATGTG ACAGCATCAT CTCCTACAAA 
GATTCCTCCC GACAGGCATT GGCAACTCCA CTGTTGAACC TTCCCCCAAA GAAAAAGAAT 
GCTGATTATT ATGAGAAGAT ATCTGACCCC TTAGATCTCA TTACCATTGA GAAGCAGATC 
CTGACAGGGT ATTACAAGAC AGTGGAGGCG TTTGATGCTG ACATGCTCAA GGTCTTCCGG 
AATGCTGAGA AATACTATGG ACGCAAGTCC CCAATTGGGA GAGATGTATG TCGACTACGC 
AAAGCATACT ACAATGCACG GCATGAGGCA TCAGCTCAAA TTGATGAGAT TGTGGGGGAA 
ACAGCAAGTG AAGCAGACAG CAGCGAAACC TCAGTCTGTG AGAAGGAAAA TGGGCATGAG 
AAGGATGATG ATGTTATCCG CTGTATCTGC GGCCTCTACA AGGATGAAGG CCTCATGATT 
CAGTGTGACA AGTGCATGGT GTGGCAGCAC TGTGATTGCA TGGGAGTAAA CTCAGATGTA 
GAACATTACC TCTGTGAACA ATGTGATCCA AGGCCTGTGG ACAGGGAAGT GCCCATGATA 
CCTCGGCCCC ATTATGCCCA ACCTGGCTGT GTCTACTTCA TCTGTCTACT CCGGGATGAC 
CTGCTGCTAC GTCAAGGTGA CTGTGTGTAC CTGATGCGAG ATAGTCGGAG AACCCCTGAT 
GGCCACCCAG TCCGTCAGTC TTATCGATTG TTATCCCATA TCAACCGAGA GAAACTTGAT 
ATCTTTCGAA TTGAGAAGCT TTGGAAGAAT GAAAAAGAGG AGCGGTTTGC CTTTGGTCAT 
CATTATTTTC GTCCCCATGA AACCCACCAC TCCCCATCAC GTCGATTTTA TCACAATGAG 
CTGTTCCGAG TGCCACTGTA TGAAATCATT CCTCTGGAGG CTGTGGTGGG GACCTGTTGC 
GTATTAGATC TGTACACATA TTGTAAAGGA AGGCCCAAAG GAGTGAAAGA GCAGGATGTA 
TATATCTGTG ACTACCGACT TGATAAATCA GCCCACCTGT TCTACAAGAT TCATCGGAAC 
CGCTATCCTG TCTGCACCAA ACCCTATGCC TTTGACCACT TCCCCAAGAA GCTCACTCCC 
AAGAGAGATT TCTCACCTCA TTATGTCCCA GACAACTACA AGAGGAATGG AGGAAGATCA 
TCCTGGAAGA CAGAACGTTC AAAGCCACCC ATCAAAGACC TAGGCCAGGA GGATGATGCC 
CTACCACTCA TTGAAGAAGT ACTGGCCAGT CAAGAGCAAG CAGCCAATGA GATGCCCACC 
CTAGAAGAAA CTGATCGGGA GGTAGCACCT GGTGAAACAA CTGAGGGTGA AAAGAAAGCT 
GATGAAAGCA GACAGGAGCC TCAGAGGTCC TGCACACCTG AAGAGAGGAG ACACAGCCAA 
CGGGAGCGAC TCAACCAGAT TCTGCTCAAC CTTCTTGAGA AGATCCCTGG GAAGAATGCC 
ATCGATGTGA CATACTTGCT GGAGGAAGGA TCAGGAAGAA AGCTTCGAAG ACGTACTCTG 
CTTATCCCAG AGAGCAGCTT CCGAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003768124.1
CDS	93..9002
Translation	MDHRNTAMLGVGTDSEGFSRKSPSAVSTGTLVSKREVELENSTKEEEDLWRRNRDRNIETGKNDGLTDAQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTSIKHPRKALKSGKMTDEKNELCPSKRDPSKLYKKANDVAAVECRSEEGIHLHSQGENNPVSKKLSPLHSEMTDYVKSAPSTLVGNRDPDSKDRALLNGATSVTEKLAQLIATCPPSKSSKTKPKKLGAGITAGLVSKDLIRKPGIGPMAGIIHKDLIKKPTISPAVGLVTKDPGKKPIFSAAVGLVSKDSGKKLGTGTTAVFINKDLGKKPGTITTVGLVSRESGKKLGIGIVPGLVSKESGKKLGLGTVVGLVNKDSVKKFGPAIGLVAKDCGKKTITNSSVGLVNKDPAKKLVACPVAGLVNKDIVNLKAEALLPVQEPLKVSCNTNTSSHESQEHSESLKDSATSKAFEKTIIRQNKDSLLEKFSIRKEIITLDKDIFNEGTCIQQDSFLSSERGTYVETTKHEKQPPVYCTSPDFQMGGTSDASTAKSPFSAIGESNLPSPSPALSVTPLNRSPPGTSSQLATNPLHLGPSTELIEGIAESIGKTQFSSENSHMNLGHRSLVHSMNTDCKGNDKELNDSKASHTDNSRMGSSIGKKPSLASESSIHATITPSVVSFTSLFSSKPFLKIGAVTASEKHCQVAESLSTSLQSKPLKKRKGRKPRWTKVVARNTCRPSKGLELERSELFKNVSCSSLSNSNSEPAKFMKNIGPSSFVEHDFLKCRLPKLSKSSTAPSLALLTETDKQSHKSFASHKLSSSICMSGDLLPDIYKPKRGRPKTKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQIRSPVKMKPPVLSVAPFVATESPSKLESESDKHRSSSDFFERKDQLQGPDDSHKPSVCGMSDLEMEPDKKINKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSTLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKAVLNGILTSSPASLAVLEKTAHQVAGSALGQILPPLLPSAASSSEVLPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHIHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFLAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPTFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLAAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPAKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDTLATNSSSRTVLESLKRYRFGKDAVGERYKHKEKHRCHMSCPHLSPSKSLLSREEQWVHREPSESTSLALGLQTPLQIDCSETSPGLSLGGFTPSSEPASSDEHTNLFTSAIGSCRVSNSTNTSGRKKLTDSPGLFTAQDTSLNRPLRKETLPSNERALQTLTGSQPVLDKPSQRPSESTNCSPTRKRSSSESTSSTVIGIPSRGTRLGTSVDDSVDSLLQRMAQHESQGTLEKNIDAVIATVSIPPSSSPGHTHGRERSLGKSDSLLVPTAPSDSCSNTTTTISLLSERLPSSCSPHHLKRSVVEAMQRQARKMCNYNKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKYPLQAVVSMQAFQAAQFASPELDEGEEGRALHLGPDTVTDVIEAVVQSVNLNPELRKGWKRKSWLLEEEARKRQKILPEEEQENNKSFPETTIESPSPPEAPPKPPEPENTLQPLPSLIPREKKAPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPDDGDRKGCMDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCNPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFDKCRGIIGGKSQRMNGLTSKSSQPVTTHRRPGRSKEKRKSKHKLKKRRGHLSEEPNENVSTPTRMTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIRQKQEEVKHTSDNIHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENIEVARAARLAQIFKEICDSIISYKDSSRQALATPLLNLPPKKKNADYYEKISDPLDLITIEKQILTGYYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKAYYNARHEASAQIDEIVGETASEADSSETSVCEKENGHEKDDDVIRCICGLYKDEGLMIQCDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINREKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKTERSKPPIKDLGQEDDALPLIEEVLASQEQAANEMPTLEETDREVAPGETTEGEKKADESRQEPQRSCTPEERRHSQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLLIPESSFRK

Target ORF information:

RefSeq Version	XM_003768076.3
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003768076.3

ORF Insert Sequence:

ATGGACCATA GGAATACTGC TATGTTAGGA GTGGGTACCG ACTCCGAAGG ATTTTCAAGG 
AAGAGTCCTT CTGCTGTCAG CACTGGAACA CTGGTTAGTA AGAGAGAAGT GGAACTAGAG 
AATAGCACTA AGGAAGAAGA GGATCTTTGG AGGCGGAATA GGGACAGAAA CATTGAGACA 
GGGAAAAATG ATGGTTTGAC TGATGCACAG CAGTTTTCTG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAGTTGAA AATTGGCCTT CAGGCTAAGA GAACTAAAAA GCCTCCTAAA 
AACTTGGAGA ACTATGTATG TCGACCTGCT ATAAAAACAA GCATTAAGCA TCCAAGGAAA 
GCATTGAAAA GTGGGAAGAT GACAGATGAA AAAAATGAAC TTTGCCCTTC AAAACGAGAC 
CCTTCAAAAC TTTACAAGAA AGCAAATGAT GTTGCAGCTG TTGAATGCCG CTCTGAAGAA 
GGCATCCACC TTCATTCACA GGGAGAAAAT AATCCTGTGT CAAAGAAGCT TTCTCCACTA 
CACTCTGAAA TGACAGATTA TGTTAAATCA GCACCATCTA CTCTTGTTGG TAACCGTGAT 
CCTGACTCGA AGGACAGAGC TCTACTTAAT GGAGCAACAA GTGTAACAGA AAAATTGGCA 
CAGCTCATTG CTACTTGTCC ACCCTCCAAG TCTTCCAAGA CAAAGCCAAA AAAGTTAGGG 
GCTGGTATTA CAGCAGGATT GGTTAGCAAA GACTTGATCA GAAAGCCAGG CATTGGTCCT 
ATGGCAGGAA TTATTCACAA GGACTTGATT AAGAAACCAA CAATTAGCCC AGCAGTTGGG 
TTGGTAACTA AAGACCCTGG GAAAAAGCCA ATCTTTAGTG CTGCAGTAGG ATTGGTCAGT 
AAAGACTCTG GGAAAAAATT AGGAACTGGG ACTACTGCGG TATTCATAAA CAAGGACTTA 
GGAAAGAAAC CAGGGACTAT CACTACAGTG GGACTGGTGA GCAGAGAGTC AGGGAAGAAA 
TTAGGAATTG GTATCGTCCC AGGTTTGGTG AGTAAAGAAT CAGGAAAGAA GTTGGGACTT 
GGTACTGTAG TTGGGCTTGT TAATAAAGAT TCAGTAAAGA AGTTTGGCCC TGCCATTGGA 
CTGGTTGCAA AGGACTGTGG GAAGAAGACT ATAACAAATT CATCAGTGGG ATTGGTTAAT 
AAGGACCCTG CAAAGAAATT GGTGGCTTGC CCTGTAGCAG GATTGGTCAA CAAAGACATT 
GTGAATTTAA AAGCTGAAGC CTTGCTCCCT GTTCAAGAAC CACTTAAGGT TTCTTGCAAT 
ACAAATACTA GTAGTCATGA AAGTCAGGAG CATTCTGAAT CCCTGAAAGA TAGTGCCACC 
AGCAAAGCTT TTGAGAAGAC TATTATTCGT CAGAATAAAG ATAGTTTATT AGAAAAGTTC 
TCAATTCGAA AAGAAATCAT TACTTTGGAC AAAGACATTT TTAATGAAGG AACTTGCATA 
CAGCAGGACA GTTTCTTATC CAGTGAAAGG GGGACCTATG TTGAAACTAC AAAGCATGAG 
AAGCAGCCTC CAGTGTATTG CACTTCACCA GATTTTCAAA TGGGAGGCAC TTCTGATGCA 
TCAACAGCTA AGTCTCCCTT TAGTGCAATA GGGGAGAGTA ATCTCCCTTC CCCTTCACCA 
GCCTTGTCTG TTACGCCCTT AAACAGAAGT CCCCCTGGAA CTTCTTCTCA GTTGGCTACT 
AATCCATTAC ATTTAGGTCC TTCCACAGAA CTCATAGAAG GAATTGCTGA ATCTATTGGA 
AAAACCCAAT TCAGTTCTGA AAATTCCCAT ATGAACCTTG GTCACAGGTC TCTGGTTCAC 
AGCATGAACA CTGATTGCAA AGGAAATGAT AAAGAGTTAA ATGATTCAAA AGCTTCTCAC 
ACAGATAACT CAAGAATGGG CTCCTCCATA GGAAAAAAGC CAAGTCTGGC TTCAGAATCC 
AGTATTCATG CTACAATTAC TCCCTCAGTT GTTAGTTTCA CTAGTTTATT TAGCAGTAAA 
CCCTTTCTGA AGATTGGTGC AGTAACTGCA TCAGAGAAAC ACTGCCAAGT TGCTGAAAGC 
CTGAGCACTA GTTTGCAGTC CAAACCATTA AAAAAAAGAA AAGGAAGGAA ACCACGATGG 
ACTAAAGTGG TAGCAAGAAA CACATGCCGG CCTTCAAAAG GGCTAGAATT AGAAAGGTCA 
GAGCTTTTTA AAAATGTTTC ATGTAGCTCT TTGTCAAATA GCAATTCCGA GCCAGCCAAG 
TTTATGAAAA ACATTGGGCC ATCTTCATTT GTGGAGCATG ACTTCCTTAA ATGCCGATTG 
CCAAAGCTGA GTAAATCTAG CACAGCTCCA TCTCTTGCTC TCTTAACTGA AACTGACAAG 
CAGTCTCATA AGTCCTTTGC CAGCCACAAA CTTTCCTCTA GTATATGCAT GTCTGGTGAC 
CTTTTACCTG ACATTTATAA ACCTAAAAGA GGAAGACCTA AAACTAAGGA GATGCCCCAA 
CTAGAAGGTC CACCCAAAAG GACCCTAAAA ATCCCGGCCT CTAAAGTTTT TTCATTACAG 
TCAAAAGAGG AGCAAGAACC CCCTATTTTG CAGCCAGAAA TTGAAATCCC TTCCTTCAAG 
CAGAGCCTAT CAGTATCACC CTTTCCCAAA AAAAGAGGAA GGCCAAAAAG ACAAATCAGA 
TCACCAGTCA AGATGAAACC ACCTGTGCTA TCAGTGGCAC CCTTTGTAGC CACTGAAAGT 
CCAAGTAAAC TAGAGTCTGA GAGTGATAAA CATAGAAGTA GTAGTGACTT CTTTGAAAGG 
AAAGATCAAC TTCAGGGTCC AGATGACAGT CATAAACCAA GTGTGTGTGG TATGAGTGAC 
CTTGAGATGG AACCTGATAA AAAAATTAAT AAGAGAAACA ATGGCCAATT AATGAAAACT 
ATCATTCGCA AAATAAATAA GATGAAGACT TTGAAGAGAA AGAAACTGCT GAATCAGATT 
CTCTCAAGTT CTGTTGAATC AAGTAACAAA GGGAAAGTGC AATCCAAACT CCACAATACA 
GTGTCAACAC TTGCTGCCAC TTTTGGCTCC AAACTGGGCC AACAGATAAA TGTCAGCAAG 
AAAGGAACCA TTTATATAGG AAAGAGGCGA GGCCGGAAAC CAAAAGCTGT GCTGAACGGC 
ATCTTAACTA GTAGCCCAGC TAGTCTGGCT GTCCTAGAGA AAACAGCTCA TCAGGTAGCT 
GGGTCAGCAT TAGGACAGAT TCTCCCCCCA CTCTTGCCTT CAGCAGCAAG TAGTTCTGAG 
GTTCTTCCAT CCCCCATCTG TTCTCAGTCT TCTGGGACTA GTGGGGGCCA GAGTCCTGTC 
AGCAGTGATG CAGGATTTGT TGAGCCCAGT TCAGTGCCAT ATTTACATAT ACACTCCAGA 
CAGGGCAGTA TGATTCAGAC ACTTGCTATG AAGAAGGCTT CAAAGGGAAG GAGACGGTTG 
TCTCCTCCTA CTTTGTTGCC AAATTCTCCT TCTCACTTGA GTGAACTCAC TTCTCTGAAG 
GAAGCTACTC CTTCCCCAAT TAGTGAATCT CATAGTGATG AGACCATTCC TAGTGATAGT 
GGAATTGGAA CAGATAATAA TAGCACATCA GACAGGGCAG AAAAGTTTTG TGGGCAGAAA 
AAGAGGAGGC ATTCTTTTGA ACATGTTTCT CTCATTCCCC CTGAAACCTC CACAGTCCTA 
AGTAGTCTCA AAGAGAAACA TAAACACAAA TGTAAACGCA GGAGTCATGA TTACCTCAGT 
TATGACAAAA TGAAAAGACA GAAGCGAAAA CGGAAAAAGA AGTATCCCCA GTTACGAAAC 
AGACAGGATC CAGACTTTCT TGCTGAGCTG GAGGAATTAA TAAGTCGTTT AAGTGAAATT 
CGGATCACTC ATCGAAGTCA TCATTTTATT CCCCGTGATC TCCTACCCAC TATTTTTCGA 
ATTAACTTTA ACAGTTTCTA TACGCACCCC ACTTTTCCCT TAGACCCTTT GCACTACATT 
AGAAAACCTG ACTTAAAGAA AAAAAGAGGG AGGCCTCCCA AGATGAGGGA AGCAATGGCT 
GAAATGCCTT TTATGCATAG CCTTAGTTTC CCTCTCTCTA GTACTGGGTT TTACCCCTCT 
TATGGCATGC CTTATTCTCC CTCTCCACTT GCAGCTGCTC CCATAGGATT AGGATACTAT 
GGAAGGTACC CTCCAACTCT TTATCCACCA CCCCCCTCTC CTTCTTTCAC CACTCCTTTG 
CCACCTCCTT CTTACATGCA TGCTGGTCAT TTGCTTCTCA ATCCTGCCAA GTACCACAAG 
AAGAAACATA AGCTGCTTCG TCAGGAAGCC TTTCTTACAA CAAGTAGGAC ACCCCTTCTC 
TCCATGAGCA CCTACCCCAG TGTCCCTCCT GAGATGGCCT ATGGTTGGAT GGTGGAGCAT 
AAACACCGGC ACCGGCACAA ACACAGAGAA CACCGTTCTT CTGAGCAACC CCAGGTTTCC 
ATGGACACTT TAGCTACCAA TAGCTCTTCC AGAACTGTCC TAGAATCGTT GAAGCGCTAT 
CGATTTGGTA AAGATGCTGT TGGGGAGCGA TATAAACACA AAGAGAAGCA TCGTTGTCAC 
ATGTCCTGTC CTCATCTGTC CCCATCAAAA AGCTTATTAA GCCGAGAGGA GCAATGGGTC 
CACCGAGAGC CTTCAGAATC CACTTCATTG GCCTTGGGGT TGCAGACACC TTTACAGATT 
GACTGTTCAG AAACGTCTCC AGGTTTGTCC CTGGGAGGTT TTACACCTAG CTCTGAGCCA 
GCCAGCAGTG ATGAACACAC AAACCTTTTC ACAAGTGCAA TAGGCAGCTG CAGAGTATCA 
AACTCTACTA ATACAAGTGG CCGGAAGAAA TTGACTGACA GTCCAGGACT CTTTACCGCA 
CAGGACACTT CATTAAATCG GCCTCTTAGA AAGGAAACTT TGCCTTCCAA TGAAAGGGCA 
CTGCAAACTT TGACAGGCTC CCAGCCAGTC CTAGACAAGC CTTCCCAAAG GCCATCAGAG 
AGCACAAACT GTAGCCCTAC TCGAAAGAGA TCTTCATCAG AGAGCACTTC TTCAACAGTG 
ATTGGAATCC CTTCCCGAGG TACAAGATTA GGAACATCTG TGGATGACTC TGTGGATAGC 
CTGCTACAGC GTATGGCGCA ACATGAAAGC CAAGGGACCT TAGAGAAAAA CATTGATGCT 
GTCATTGCCA CAGTGTCCAT CCCACCTTCC TCAAGCCCAG GTCATACTCA TGGTAGGGAA 
CGAAGTCTGG GGAAATCAGA CAGTCTGCTA GTACCCACAG CACCTAGTGA CTCCTGCAGC 
AACACCACCA CCACCATCTC ACTCCTTTCT GAGAGGTTGC CAAGCAGCTG TTCCCCCCAT 
CATCTCAAAA GGAGTGTGGT AGAAGCCATG CAACGCCAAG CCCGGAAAAT GTGCAATTAT 
AACAAAATCT TGGCCACCAA GAAAAACCTA GACCATGTCA ATAAAATCTT AAAAGCCAAA 
AAGCTTCAAA GACAGGCAAG GACAGGTAAC AATTTTGTGA AGCGCAGACC AGGCCGACCC 
CGGAAATATC CCCTCCAGGC TGTGGTGTCA ATGCAGGCAT TCCAGGCTGC CCAATTTGCC 
AGCCCAGAAT TAGATGAAGG GGAGGAAGGC AGAGCACTAC ATCTTGGTCC TGATACAGTT 
ACAGATGTCA TTGAGGCTGT TGTTCAGAGT GTGAACCTGA ACCCAGAACT TAGAAAAGGA 
TGGAAAAGGA AAAGTTGGCT TCTGGAGGAA GAGGCAAGGA AAAGACAGAA GATACTTCCA 
GAGGAAGAAC AGGAAAATAA TAAAAGCTTT CCTGAGACAA CAATTGAATC TCCTAGTCCT 
CCAGAAGCCC CACCAAAGCC ACCTGAACCT GAAAATACCC TGCAGCCTTT GCCTTCTCTT 
ATCCCTCGGG AAAAGAAGGC CCCACGTCCC CCAAAGAAGA AATATCAGAA AGCAGGGCTC 
TATTCTGATG TATACAAAAC TACAGATCCA AAGAGCAGAC TGATACAATT AAAGAAAGAA 
AAACTGGAGT ATACTCCTGG AGAACATGAA TATGGATTAT TCCCAGCCCC TATTCATGTT 
GGAAAGTATT TGAGACAAAA GAGAATTGAC TTTCAGCTTC CTTATGACAT CCTCTGGCAA 
TGGAAACACA ATCAGCTATA TAAAAAGCCA GATGTCCCAC TATACAAAAA AATCCGTTCA 
AATGTCTACG TTGATGTCAA ACCTCTTTCT GGCTATGAGG CTACCACCTG CAACTGTAAA 
AAACCAGATG ATGGTGATAG GAAGGGTTGT ATGGATGACT GCCTCAACAG AATGATCTTT 
GCTGAGTGTT CCCCCAATAC CTGCCCCTGT GGAGAGCAGT GCTGCAATCA GAGGATACAA 
AGGCATGAGT GGGTACAGTG TCTTGAGCGG TTCCGAGCTG AGGAGAAGGG ATGGGGGATC 
CGGACCAAAG AACCTTTGAA AGCTGGCCAG TTCATTATTG AATACTTGGG AGAAGTTGTC 
AGTGAGCAGG AGTTCAGAAA CCGAATGATT GAACAGTACC ATAATCACAG TGACCATTAC 
TGCCTAAATC TGGACAGTGG AATGGTGATT GACAGCTACC GAATGGGCAA TGAGGCCCGG 
TTCATCAACC ACAGCTGTAA CCCAAATTGT GAAATGCAGA AATGGTCTGT TAATGGAGTC 
TACCGGATTG GGCTCTATGC CCTGAAAGAT ATGCCAGCTG GTACTGAACT AACCTATGAC 
TACAATTTTC ATTCTTTTAA TGTTGAGAAA CAGCAACTTT GTAAGTGTGG TTTTGACAAA 
TGCCGGGGTA TCATCGGAGG CAAGAGCCAA CGCATGAATG GACTCACCAG CAAAAGCAGC 
CAACCTGTGA CCACACACAG ACGGCCAGGA CGGTCAAAAG AGAAGAGAAA ATCCAAGCAC 
AAGTTGAAGA AAAGGAGAGG CCATCTTTCT GAGGAGCCTA ATGAAAATGT CAGTACCCCA 
ACCAGAATGA CCCCCCAACT GCAAATGAAG CCAATGTCCA ACCGTGAAAG GAACTTTGTG 
TTAAAACATC ATGTATTCCT GGTCCGGAAC TGGGAGAAAA TCAGACAGAA GCAAGAGGAG 
GTAAAACACA CCAGTGACAA TATCCATTCA GCTTCCTTAT ATACCCGCTG GAATGGTATC 
TGCCGAGATG ATGGAAACAT CAAATCTGAT GTCTTCATGA CCCAGTTCTC CGCCTTGCAG 
ACAGCCCGCT CAGTTCGTAC AAGGAGATTG GCAGCTGCAG AGGAGAACAT TGAAGTGGCC 
CGGGCAGCCC GCCTGGCCCA GATATTCAAA GAGATATGTG ACAGCATCAT CTCCTACAAA 
GATTCCTCCC GACAGGCATT GGCAACTCCA CTGTTGAACC TTCCCCCAAA GAAAAAGAAT 
GCTGATTATT ATGAGAAGAT ATCTGACCCC TTAGATCTCA TTACCATTGA GAAGCAGATC 
CTGACAGGGT ATTACAAGAC AGTGGAGGCG TTTGATGCTG ACATGCTCAA GGTCTTCCGG 
AATGCTGAGA AATACTATGG ACGCAAGTCC CCAATTGGGA GAGATGTATG TCGACTACGC 
AAAGCATACT ACAATGCACG GCATGAGGCA TCAGCTCAAA TTGATGAGAT TGTGGGGGAA 
ACAGCAAGTG AAGCAGACAG CAGCGAAACC TCAGTCTGTG AGAAGGAAAA TGGGCATGAG 
AAGGATGATG ATGTTATCCG CTGTATCTGC GGCCTCTACA AGGATGAAGG CCTCATGATT 
CAGTGTGACA AGTGCATGGT GTGGCAGCAC TGTGATTGCA TGGGAGTAAA CTCAGATGTA 
GAACATTACC TCTGTGAACA ATGTGATCCA AGGCCTGTGG ACAGGGAAGT GCCCATGATA 
CCTCGGCCCC ATTATGCCCA ACCTGGCTGT GTCTACTTCA TCTGTCTACT CCGGGATGAC 
CTGCTGCTAC GTCAAGGTGA CTGTGTGTAC CTGATGCGAG ATAGTCGGAG AACCCCTGAT 
GGCCACCCAG TCCGTCAGTC TTATCGATTG TTATCCCATA TCAACCGAGA GAAACTTGAT 
ATCTTTCGAA TTGAGAAGCT TTGGAAGAAT GAAAAAGAGG AGCGGTTTGC CTTTGGTCAT 
CATTATTTTC GTCCCCATGA AACCCACCAC TCCCCATCAC GTCGATTTTA TCACAATGAG 
CTGTTCCGAG TGCCACTGTA TGAAATCATT CCTCTGGAGG CTGTGGTGGG GACCTGTTGC 
GTATTAGATC TGTACACATA TTGTAAAGGA AGGCCCAAAG GAGTGAAAGA GCAGGATGTA 
TATATCTGTG ACTACCGACT TGATAAATCA GCCCACCTGT TCTACAAGAT TCATCGGAAC 
CGCTATCCTG TCTGCACCAA ACCCTATGCC TTTGACCACT TCCCCAAGAA GCTCACTCCC 
AAGAGAGATT TCTCACCTCA TTATGTCCCA GACAACTACA AGAGGAATGG AGGAAGATCA 
TCCTGGAAGA CAGAACGTTC AAAGCCACCC ATCAAAGACC TAGGCCAGGA GGATGATGCC 
CTACCACTCA TTGAAGAAGT ACTGGCCAGT CAAGAGCAAG CAGCCAATGA GATGCCCACC 
CTAGAAGAAA CTGATCGGGA GGTAGCACCT GGTGAAACAA CTGAGGGTGA AAAGAAAGCT 
GATGAAAGCA GACAGGAGCC TCAGAGGTCC TGCACACCTG AAGAGAGGAG ACACAGCCAA 
CGGGAGCGAC TCAACCAGAT TCTGCTCAAC CTTCTTGAGA AGATCCCTGG GAAGAATGCC 
ATCGATGTGA CATACTTGCT GGAGGAAGGA TCAGGAAGAA AGCTTCGAAG ACGTACTCTG 
CTTATCCCAG AGAGCAGCTT CCGAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASH1L ( XM_031966723.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031822583.1 Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X5, mRNA.

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CloneID	OSa130098
Clone ID Related Accession (Same CDS sequence)	XM_003768076.3 , XM_031966723.1 , XM_003768076.2
Accession Version	XM_031966723.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	histone-lysine N-methyltransferase ASH1L isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATA GGAATACTGC TATGTTAGGA GTGGGTACCG ACTCCGAAGG ATTTTCAAGG 
AAGAGTCCTT CTGCTGTCAG CACTGGAACA CTGGTTAGTA AGAGAGAAGT GGAACTAGAG 
AATAGCACTA AGGAAGAAGA GGATCTTTGG AGGCGGAATA GGGACAGAAA CATTGAGACA 
GGGAAAAATG ATGGTTTGAC TGATGCACAG CAGTTTTCTG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAGTTGAA AATTGGCCTT CAGGCTAAGA GAACTAAAAA GCCTCCTAAA 
AACTTGGAGA ACTATGTATG TCGACCTGCT ATAAAAACAA GCATTAAGCA TCCAAGGAAA 
GCATTGAAAA GTGGGAAGAT GACAGATGAA AAAAATGAAC TTTGCCCTTC AAAACGAGAC 
CCTTCAAAAC TTTACAAGAA AGCAAATGAT GTTGCAGCTG TTGAATGCCG CTCTGAAGAA 
GGCATCCACC TTCATTCACA GGGAGAAAAT AATCCTGTGT CAAAGAAGCT TTCTCCACTA 
CACTCTGAAA TGACAGATTA TGTTAAATCA GCACCATCTA CTCTTGTTGG TAACCGTGAT 
CCTGACTCGA AGGACAGAGC TCTACTTAAT GGAGCAACAA GTGTAACAGA AAAATTGGCA 
CAGCTCATTG CTACTTGTCC ACCCTCCAAG TCTTCCAAGA CAAAGCCAAA AAAGTTAGGG 
GCTGGTATTA CAGCAGGATT GGTTAGCAAA GACTTGATCA GAAAGCCAGG CATTGGTCCT 
ATGGCAGGAA TTATTCACAA GGACTTGATT AAGAAACCAA CAATTAGCCC AGCAGTTGGG 
TTGGTAACTA AAGACCCTGG GAAAAAGCCA ATCTTTAGTG CTGCAGTAGG ATTGGTCAGT 
AAAGACTCTG GGAAAAAATT AGGAACTGGG ACTACTGCGG TATTCATAAA CAAGGACTTA 
GGAAAGAAAC CAGGGACTAT CACTACAGTG GGACTGGTGA GCAGAGAGTC AGGGAAGAAA 
TTAGGAATTG GTATCGTCCC AGGTTTGGTG AGTAAAGAAT CAGGAAAGAA GTTGGGACTT 
GGTACTGTAG TTGGGCTTGT TAATAAAGAT TCAGTAAAGA AGTTTGGCCC TGCCATTGGA 
CTGGTTGCAA AGGACTGTGG GAAGAAGACT ATAACAAATT CATCAGTGGG ATTGGTTAAT 
AAGGACCCTG CAAAGAAATT GGTGGCTTGC CCTGTAGCAG GATTGGTCAA CAAAGACATT 
GTGAATTTAA AAGCTGAAGC CTTGCTCCCT GTTCAAGAAC CACTTAAGGT TTCTTGCAAT 
ACAAATACTA GTAGTCATGA AAGTCAGGAG CATTCTGAAT CCCTGAAAGA TAGTGCCACC 
AGCAAAGCTT TTGAGAAGAC TATTATTCGT CAGAATAAAG ATAGTTTATT AGAAAAGTTC 
TCAATTCGAA AAGAAATCAT TACTTTGGAC AAAGACATTT TTAATGAAGG AACTTGCATA 
CAGCAGGACA GTTTCTTATC CAGTGAAAGG GGGACCTATG TTGAAACTAC AAAGCATGAG 
AAGCAGCCTC CAGTGTATTG CACTTCACCA GATTTTCAAA TGGGAGGCAC TTCTGATGCA 
TCAACAGCTA AGTCTCCCTT TAGTGCAATA GGGGAGAGTA ATCTCCCTTC CCCTTCACCA 
GCCTTGTCTG TTACGCCCTT AAACAGAAGT CCCCCTGGAA CTTCTTCTCA GTTGGCTACT 
AATCCATTAC ATTTAGGTCC TTCCACAGAA CTCATAGAAG GAATTGCTGA ATCTATTGGA 
AAAACCCAAT TCAGTTCTGA AAATTCCCAT ATGAACCTTG GTCACAGGTC TCTGGTTCAC 
AGCATGAACA CTGATTGCAA AGGAAATGAT AAAGAGTTAA ATGATTCAAA AGCTTCTCAC 
ACAGATAACT CAAGAATGGG CTCCTCCATA GGAAAAAAGC CAAGTCTGGC TTCAGAATCC 
AGTATTCATG CTACAATTAC TCCCTCAGTT GTTAGTTTCA CTAGTTTATT TAGCAGTAAA 
CCCTTTCTGA AGATTGGTGC AGTAACTGCA TCAGAGAAAC ACTGCCAAGT TGCTGAAAGC 
CTGAGCACTA GTTTGCAGTC CAAACCATTA AAAAAAAGAA AAGGAAGGAA ACCACGATGG 
ACTAAAGTGG TAGCAAGAAA CACATGCCGG CCTTCAAAAG GGCTAGAATT AGAAAGGTCA 
GAGCTTTTTA AAAATGTTTC ATGTAGCTCT TTGTCAAATA GCAATTCCGA GCCAGCCAAG 
TTTATGAAAA ACATTGGGCC ATCTTCATTT GTGGAGCATG ACTTCCTTAA ATGCCGATTG 
CCAAAGCTGA GTAAATCTAG CACAGCTCCA TCTCTTGCTC TCTTAACTGA AACTGACAAG 
CAGTCTCATA AGTCCTTTGC CAGCCACAAA CTTTCCTCTA GTATATGCAT GTCTGGTGAC 
CTTTTACCTG ACATTTATAA ACCTAAAAGA GGAAGACCTA AAACTAAGGA GATGCCCCAA 
CTAGAAGGTC CACCCAAAAG GACCCTAAAA ATCCCGGCCT CTAAAGTTTT TTCATTACAG 
TCAAAAGAGG AGCAAGAACC CCCTATTTTG CAGCCAGAAA TTGAAATCCC TTCCTTCAAG 
CAGAGCCTAT CAGTATCACC CTTTCCCAAA AAAAGAGGAA GGCCAAAAAG ACAAATCAGA 
TCACCAGTCA AGATGAAACC ACCTGTGCTA TCAGTGGCAC CCTTTGTAGC CACTGAAAGT 
CCAAGTAAAC TAGAGTCTGA GAGTGATAAA CATAGAAGTA GTAGTGACTT CTTTGAAAGG 
AAAGATCAAC TTCAGGGTCC AGATGACAGT CATAAACCAA GTGTGTGTGG TATGAGTGAC 
CTTGAGATGG AACCTGATAA AAAAATTAAT AAGAGAAACA ATGGCCAATT AATGAAAACT 
ATCATTCGCA AAATAAATAA GATGAAGACT TTGAAGAGAA AGAAACTGCT GAATCAGATT 
CTCTCAAGTT CTGTTGAATC AAGTAACAAA GGGAAAGTGC AATCCAAACT CCACAATACA 
GTGTCAACAC TTGCTGCCAC TTTTGGCTCC AAACTGGGCC AACAGATAAA TGTCAGCAAG 
AAAGGAACCA TTTATATAGG AAAGAGGCGA GGCCGGAAAC CAAAAGCTGT GCTGAACGGC 
ATCTTAACTA GTAGCCCAGC TAGTCTGGCT GTCCTAGAGA AAACAGCTCA TCAGGTAGCT 
GGGTCAGCAT TAGGACAGAT TCTCCCCCCA CTCTTGCCTT CAGCAGCAAG TAGTTCTGAG 
GTTCTTCCAT CCCCCATCTG TTCTCAGTCT TCTGGGACTA GTGGGGGCCA GAGTCCTGTC 
AGCAGTGATG CAGGATTTGT TGAGCCCAGT TCAGTGCCAT ATTTACATAT ACACTCCAGA 
CAGGGCAGTA TGATTCAGAC ACTTGCTATG AAGAAGGCTT CAAAGGGAAG GAGACGGTTG 
TCTCCTCCTA CTTTGTTGCC AAATTCTCCT TCTCACTTGA GTGAACTCAC TTCTCTGAAG 
GAAGCTACTC CTTCCCCAAT TAGTGAATCT CATAGTGATG AGACCATTCC TAGTGATAGT 
GGAATTGGAA CAGATAATAA TAGCACATCA GACAGGGCAG AAAAGTTTTG TGGGCAGAAA 
AAGAGGAGGC ATTCTTTTGA ACATGTTTCT CTCATTCCCC CTGAAACCTC CACAGTCCTA 
AGTAGTCTCA AAGAGAAACA TAAACACAAA TGTAAACGCA GGAGTCATGA TTACCTCAGT 
TATGACAAAA TGAAAAGACA GAAGCGAAAA CGGAAAAAGA AGTATCCCCA GTTACGAAAC 
AGACAGGATC CAGACTTTCT TGCTGAGCTG GAGGAATTAA TAAGTCGTTT AAGTGAAATT 
CGGATCACTC ATCGAAGTCA TCATTTTATT CCCCGTGATC TCCTACCCAC TATTTTTCGA 
ATTAACTTTA ACAGTTTCTA TACGCACCCC ACTTTTCCCT TAGACCCTTT GCACTACATT 
AGAAAACCTG ACTTAAAGAA AAAAAGAGGG AGGCCTCCCA AGATGAGGGA AGCAATGGCT 
GAAATGCCTT TTATGCATAG CCTTAGTTTC CCTCTCTCTA GTACTGGGTT TTACCCCTCT 
TATGGCATGC CTTATTCTCC CTCTCCACTT GCAGCTGCTC CCATAGGATT AGGATACTAT 
GGAAGGTACC CTCCAACTCT TTATCCACCA CCCCCCTCTC CTTCTTTCAC CACTCCTTTG 
CCACCTCCTT CTTACATGCA TGCTGGTCAT TTGCTTCTCA ATCCTGCCAA GTACCACAAG 
AAGAAACATA AGCTGCTTCG TCAGGAAGCC TTTCTTACAA CAAGTAGGAC ACCCCTTCTC 
TCCATGAGCA CCTACCCCAG TGTCCCTCCT GAGATGGCCT ATGGTTGGAT GGTGGAGCAT 
AAACACCGGC ACCGGCACAA ACACAGAGAA CACCGTTCTT CTGAGCAACC CCAGGTTTCC 
ATGGACACTT TAGCTACCAA TAGCTCTTCC AGAACTGTCC TAGAATCGTT GAAGCGCTAT 
CGATTTGGTA AAGATGCTGT TGGGGAGCGA TATAAACACA AAGAGAAGCA TCGTTGTCAC 
ATGTCCTGTC CTCATCTGTC CCCATCAAAA AGCTTATTAA GCCGAGAGGA GCAATGGGTC 
CACCGAGAGC CTTCAGAATC CACTTCATTG GCCTTGGGGT TGCAGACACC TTTACAGATT 
GACTGTTCAG AAACGTCTCC AGGTTTGTCC CTGGGAGGTT TTACACCTAG CTCTGAGCCA 
GCCAGCAGTG ATGAACACAC AAACCTTTTC ACAAGTGCAA TAGGCAGCTG CAGAGTATCA 
AACTCTACTA ATACAAGTGG CCGGAAGAAA TTGACTGACA GTCCAGGACT CTTTACCGCA 
CAGGACACTT CATTAAATCG GCCTCTTAGA AAGGAAACTT TGCCTTCCAA TGAAAGGGCA 
CTGCAAACTT TGACAGGCTC CCAGCCAGTC CTAGACAAGC CTTCCCAAAG GCCATCAGAG 
AGCACAAACT GTAGCCCTAC TCGAAAGAGA TCTTCATCAG AGAGCACTTC TTCAACAGTG 
ATTGGAATCC CTTCCCGAGG TACAAGATTA GGAACATCTG TGGATGACTC TGTGGATAGC 
CTGCTACAGC GTATGGCGCA ACATGAAAGC CAAGGGACCT TAGAGAAAAA CATTGATGCT 
GTCATTGCCA CAGTGTCCAT CCCACCTTCC TCAAGCCCAG GTCATACTCA TGGTAGGGAA 
CGAAGTCTGG GGAAATCAGA CAGTCTGCTA GTACCCACAG CACCTAGTGA CTCCTGCAGC 
AACACCACCA CCACCATCTC ACTCCTTTCT GAGAGGTTGC CAAGCAGCTG TTCCCCCCAT 
CATCTCAAAA GGAGTGTGGT AGAAGCCATG CAACGCCAAG CCCGGAAAAT GTGCAATTAT 
AACAAAATCT TGGCCACCAA GAAAAACCTA GACCATGTCA ATAAAATCTT AAAAGCCAAA 
AAGCTTCAAA GACAGGCAAG GACAGGTAAC AATTTTGTGA AGCGCAGACC AGGCCGACCC 
CGGAAATATC CCCTCCAGGC TGTGGTGTCA ATGCAGGCAT TCCAGGCTGC CCAATTTGCC 
AGCCCAGAAT TAGATGAAGG GGAGGAAGGC AGAGCACTAC ATCTTGGTCC TGATACAGTT 
ACAGATGTCA TTGAGGCTGT TGTTCAGAGT GTGAACCTGA ACCCAGAACT TAGAAAAGGA 
TGGAAAAGGA AAAGTTGGCT TCTGGAGGAA GAGGCAAGGA AAAGACAGAA GATACTTCCA 
GAGGAAGAAC AGGAAAATAA TAAAAGCTTT CCTGAGACAA CAATTGAATC TCCTAGTCCT 
CCAGAAGCCC CACCAAAGCC ACCTGAACCT GAAAATACCC TGCAGCCTTT GCCTTCTCTT 
ATCCCTCGGG AAAAGAAGGC CCCACGTCCC CCAAAGAAGA AATATCAGAA AGCAGGGCTC 
TATTCTGATG TATACAAAAC TACAGATCCA AAGAGCAGAC TGATACAATT AAAGAAAGAA 
AAACTGGAGT ATACTCCTGG AGAACATGAA TATGGATTAT TCCCAGCCCC TATTCATGTT 
GGAAAGTATT TGAGACAAAA GAGAATTGAC TTTCAGCTTC CTTATGACAT CCTCTGGCAA 
TGGAAACACA ATCAGCTATA TAAAAAGCCA GATGTCCCAC TATACAAAAA AATCCGTTCA 
AATGTCTACG TTGATGTCAA ACCTCTTTCT GGCTATGAGG CTACCACCTG CAACTGTAAA 
AAACCAGATG ATGGTGATAG GAAGGGTTGT ATGGATGACT GCCTCAACAG AATGATCTTT 
GCTGAGTGTT CCCCCAATAC CTGCCCCTGT GGAGAGCAGT GCTGCAATCA GAGGATACAA 
AGGCATGAGT GGGTACAGTG TCTTGAGCGG TTCCGAGCTG AGGAGAAGGG ATGGGGGATC 
CGGACCAAAG AACCTTTGAA AGCTGGCCAG TTCATTATTG AATACTTGGG AGAAGTTGTC 
AGTGAGCAGG AGTTCAGAAA CCGAATGATT GAACAGTACC ATAATCACAG TGACCATTAC 
TGCCTAAATC TGGACAGTGG AATGGTGATT GACAGCTACC GAATGGGCAA TGAGGCCCGG 
TTCATCAACC ACAGCTGTAA CCCAAATTGT GAAATGCAGA AATGGTCTGT TAATGGAGTC 
TACCGGATTG GGCTCTATGC CCTGAAAGAT ATGCCAGCTG GTACTGAACT AACCTATGAC 
TACAATTTTC ATTCTTTTAA TGTTGAGAAA CAGCAACTTT GTAAGTGTGG TTTTGACAAA 
TGCCGGGGTA TCATCGGAGG CAAGAGCCAA CGCATGAATG GACTCACCAG CAAAAGCAGC 
CAACCTGTGA CCACACACAG ACGGCCAGGA CGGTCAAAAG AGAAGAGAAA ATCCAAGCAC 
AAGTTGAAGA AAAGGAGAGG CCATCTTTCT GAGGAGCCTA ATGAAAATGT CAGTACCCCA 
ACCAGAATGA CCCCCCAACT GCAAATGAAG CCAATGTCCA ACCGTGAAAG GAACTTTGTG 
TTAAAACATC ATGTATTCCT GGTCCGGAAC TGGGAGAAAA TCAGACAGAA GCAAGAGGAG 
GTAAAACACA CCAGTGACAA TATCCATTCA GCTTCCTTAT ATACCCGCTG GAATGGTATC 
TGCCGAGATG ATGGAAACAT CAAATCTGAT GTCTTCATGA CCCAGTTCTC CGCCTTGCAG 
ACAGCCCGCT CAGTTCGTAC AAGGAGATTG GCAGCTGCAG AGGAGAACAT TGAAGTGGCC 
CGGGCAGCCC GCCTGGCCCA GATATTCAAA GAGATATGTG ACAGCATCAT CTCCTACAAA 
GATTCCTCCC GACAGGCATT GGCAACTCCA CTGTTGAACC TTCCCCCAAA GAAAAAGAAT 
GCTGATTATT ATGAGAAGAT ATCTGACCCC TTAGATCTCA TTACCATTGA GAAGCAGATC 
CTGACAGGGT ATTACAAGAC AGTGGAGGCG TTTGATGCTG ACATGCTCAA GGTCTTCCGG 
AATGCTGAGA AATACTATGG ACGCAAGTCC CCAATTGGGA GAGATGTATG TCGACTACGC 
AAAGCATACT ACAATGCACG GCATGAGGCA TCAGCTCAAA TTGATGAGAT TGTGGGGGAA 
ACAGCAAGTG AAGCAGACAG CAGCGAAACC TCAGTCTGTG AGAAGGAAAA TGGGCATGAG 
AAGGATGATG ATGTTATCCG CTGTATCTGC GGCCTCTACA AGGATGAAGG CCTCATGATT 
CAGTGTGACA AGTGCATGGT GTGGCAGCAC TGTGATTGCA TGGGAGTAAA CTCAGATGTA 
GAACATTACC TCTGTGAACA ATGTGATCCA AGGCCTGTGG ACAGGGAAGT GCCCATGATA 
CCTCGGCCCC ATTATGCCCA ACCTGGCTGT GTCTACTTCA TCTGTCTACT CCGGGATGAC 
CTGCTGCTAC GTCAAGGTGA CTGTGTGTAC CTGATGCGAG ATAGTCGGAG AACCCCTGAT 
GGCCACCCAG TCCGTCAGTC TTATCGATTG TTATCCCATA TCAACCGAGA GAAACTTGAT 
ATCTTTCGAA TTGAGAAGCT TTGGAAGAAT GAAAAAGAGG AGCGGTTTGC CTTTGGTCAT 
CATTATTTTC GTCCCCATGA AACCCACCAC TCCCCATCAC GTCGATTTTA TCACAATGAG 
CTGTTCCGAG TGCCACTGTA TGAAATCATT CCTCTGGAGG CTGTGGTGGG GACCTGTTGC 
GTATTAGATC TGTACACATA TTGTAAAGGA AGGCCCAAAG GAGTGAAAGA GCAGGATGTA 
TATATCTGTG ACTACCGACT TGATAAATCA GCCCACCTGT TCTACAAGAT TCATCGGAAC 
CGCTATCCTG TCTGCACCAA ACCCTATGCC TTTGACCACT TCCCCAAGAA GCTCACTCCC 
AAGAGAGATT TCTCACCTCA TTATGTCCCA GACAACTACA AGAGGAATGG AGGAAGATCA 
TCCTGGAAGA CAGAACGTTC AAAGCCACCC ATCAAAGACC TAGGCCAGGA GGATGATGCC 
CTACCACTCA TTGAAGAAGT ACTGGCCAGT CAAGAGCAAG CAGCCAATGA GATGCCCACC 
CTAGAAGAAA CTGATCGGGA GGTAGCACCT GGTGAAACAA CTGAGGGTGA AAAGAAAGCT 
GATGAAAGCA GACAGGAGCC TCAGAGGTCC TGCACACCTG AAGAGAGGAG ACACAGCCAA 
CGGGAGCGAC TCAACCAGAT TCTGCTCAAC CTTCTTGAGA AGATCCCTGG GAAGAATGCC 
ATCGATGTGA CATACTTGCT GGAGGAAGGA TCAGGAAGAA AGCTTCGAAG ACGTACTCTG 
CTTATCCCAG AGAGCAGCTT CCGAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031822583.1
CDS	502..9411
Translation	MDHRNTAMLGVGTDSEGFSRKSPSAVSTGTLVSKREVELENSTKEEEDLWRRNRDRNIETGKNDGLTDAQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTSIKHPRKALKSGKMTDEKNELCPSKRDPSKLYKKANDVAAVECRSEEGIHLHSQGENNPVSKKLSPLHSEMTDYVKSAPSTLVGNRDPDSKDRALLNGATSVTEKLAQLIATCPPSKSSKTKPKKLGAGITAGLVSKDLIRKPGIGPMAGIIHKDLIKKPTISPAVGLVTKDPGKKPIFSAAVGLVSKDSGKKLGTGTTAVFINKDLGKKPGTITTVGLVSRESGKKLGIGIVPGLVSKESGKKLGLGTVVGLVNKDSVKKFGPAIGLVAKDCGKKTITNSSVGLVNKDPAKKLVACPVAGLVNKDIVNLKAEALLPVQEPLKVSCNTNTSSHESQEHSESLKDSATSKAFEKTIIRQNKDSLLEKFSIRKEIITLDKDIFNEGTCIQQDSFLSSERGTYVETTKHEKQPPVYCTSPDFQMGGTSDASTAKSPFSAIGESNLPSPSPALSVTPLNRSPPGTSSQLATNPLHLGPSTELIEGIAESIGKTQFSSENSHMNLGHRSLVHSMNTDCKGNDKELNDSKASHTDNSRMGSSIGKKPSLASESSIHATITPSVVSFTSLFSSKPFLKIGAVTASEKHCQVAESLSTSLQSKPLKKRKGRKPRWTKVVARNTCRPSKGLELERSELFKNVSCSSLSNSNSEPAKFMKNIGPSSFVEHDFLKCRLPKLSKSSTAPSLALLTETDKQSHKSFASHKLSSSICMSGDLLPDIYKPKRGRPKTKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQIRSPVKMKPPVLSVAPFVATESPSKLESESDKHRSSSDFFERKDQLQGPDDSHKPSVCGMSDLEMEPDKKINKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSTLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKAVLNGILTSSPASLAVLEKTAHQVAGSALGQILPPLLPSAASSSEVLPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHIHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFLAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPTFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLAAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPAKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDTLATNSSSRTVLESLKRYRFGKDAVGERYKHKEKHRCHMSCPHLSPSKSLLSREEQWVHREPSESTSLALGLQTPLQIDCSETSPGLSLGGFTPSSEPASSDEHTNLFTSAIGSCRVSNSTNTSGRKKLTDSPGLFTAQDTSLNRPLRKETLPSNERALQTLTGSQPVLDKPSQRPSESTNCSPTRKRSSSESTSSTVIGIPSRGTRLGTSVDDSVDSLLQRMAQHESQGTLEKNIDAVIATVSIPPSSSPGHTHGRERSLGKSDSLLVPTAPSDSCSNTTTTISLLSERLPSSCSPHHLKRSVVEAMQRQARKMCNYNKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKYPLQAVVSMQAFQAAQFASPELDEGEEGRALHLGPDTVTDVIEAVVQSVNLNPELRKGWKRKSWLLEEEARKRQKILPEEEQENNKSFPETTIESPSPPEAPPKPPEPENTLQPLPSLIPREKKAPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPDDGDRKGCMDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCNPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFDKCRGIIGGKSQRMNGLTSKSSQPVTTHRRPGRSKEKRKSKHKLKKRRGHLSEEPNENVSTPTRMTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIRQKQEEVKHTSDNIHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENIEVARAARLAQIFKEICDSIISYKDSSRQALATPLLNLPPKKKNADYYEKISDPLDLITIEKQILTGYYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKAYYNARHEASAQIDEIVGETASEADSSETSVCEKENGHEKDDDVIRCICGLYKDEGLMIQCDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINREKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKTERSKPPIKDLGQEDDALPLIEEVLASQEQAANEMPTLEETDREVAPGETTEGEKKADESRQEPQRSCTPEERRHSQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLLIPESSFRK

Target ORF information:

RefSeq Version	XM_031966723.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031966723.1

ORF Insert Sequence:

ATGGACCATA GGAATACTGC TATGTTAGGA GTGGGTACCG ACTCCGAAGG ATTTTCAAGG 
AAGAGTCCTT CTGCTGTCAG CACTGGAACA CTGGTTAGTA AGAGAGAAGT GGAACTAGAG 
AATAGCACTA AGGAAGAAGA GGATCTTTGG AGGCGGAATA GGGACAGAAA CATTGAGACA 
GGGAAAAATG ATGGTTTGAC TGATGCACAG CAGTTTTCTG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAGTTGAA AATTGGCCTT CAGGCTAAGA GAACTAAAAA GCCTCCTAAA 
AACTTGGAGA ACTATGTATG TCGACCTGCT ATAAAAACAA GCATTAAGCA TCCAAGGAAA 
GCATTGAAAA GTGGGAAGAT GACAGATGAA AAAAATGAAC TTTGCCCTTC AAAACGAGAC 
CCTTCAAAAC TTTACAAGAA AGCAAATGAT GTTGCAGCTG TTGAATGCCG CTCTGAAGAA 
GGCATCCACC TTCATTCACA GGGAGAAAAT AATCCTGTGT CAAAGAAGCT TTCTCCACTA 
CACTCTGAAA TGACAGATTA TGTTAAATCA GCACCATCTA CTCTTGTTGG TAACCGTGAT 
CCTGACTCGA AGGACAGAGC TCTACTTAAT GGAGCAACAA GTGTAACAGA AAAATTGGCA 
CAGCTCATTG CTACTTGTCC ACCCTCCAAG TCTTCCAAGA CAAAGCCAAA AAAGTTAGGG 
GCTGGTATTA CAGCAGGATT GGTTAGCAAA GACTTGATCA GAAAGCCAGG CATTGGTCCT 
ATGGCAGGAA TTATTCACAA GGACTTGATT AAGAAACCAA CAATTAGCCC AGCAGTTGGG 
TTGGTAACTA AAGACCCTGG GAAAAAGCCA ATCTTTAGTG CTGCAGTAGG ATTGGTCAGT 
AAAGACTCTG GGAAAAAATT AGGAACTGGG ACTACTGCGG TATTCATAAA CAAGGACTTA 
GGAAAGAAAC CAGGGACTAT CACTACAGTG GGACTGGTGA GCAGAGAGTC AGGGAAGAAA 
TTAGGAATTG GTATCGTCCC AGGTTTGGTG AGTAAAGAAT CAGGAAAGAA GTTGGGACTT 
GGTACTGTAG TTGGGCTTGT TAATAAAGAT TCAGTAAAGA AGTTTGGCCC TGCCATTGGA 
CTGGTTGCAA AGGACTGTGG GAAGAAGACT ATAACAAATT CATCAGTGGG ATTGGTTAAT 
AAGGACCCTG CAAAGAAATT GGTGGCTTGC CCTGTAGCAG GATTGGTCAA CAAAGACATT 
GTGAATTTAA AAGCTGAAGC CTTGCTCCCT GTTCAAGAAC CACTTAAGGT TTCTTGCAAT 
ACAAATACTA GTAGTCATGA AAGTCAGGAG CATTCTGAAT CCCTGAAAGA TAGTGCCACC 
AGCAAAGCTT TTGAGAAGAC TATTATTCGT CAGAATAAAG ATAGTTTATT AGAAAAGTTC 
TCAATTCGAA AAGAAATCAT TACTTTGGAC AAAGACATTT TTAATGAAGG AACTTGCATA 
CAGCAGGACA GTTTCTTATC CAGTGAAAGG GGGACCTATG TTGAAACTAC AAAGCATGAG 
AAGCAGCCTC CAGTGTATTG CACTTCACCA GATTTTCAAA TGGGAGGCAC TTCTGATGCA 
TCAACAGCTA AGTCTCCCTT TAGTGCAATA GGGGAGAGTA ATCTCCCTTC CCCTTCACCA 
GCCTTGTCTG TTACGCCCTT AAACAGAAGT CCCCCTGGAA CTTCTTCTCA GTTGGCTACT 
AATCCATTAC ATTTAGGTCC TTCCACAGAA CTCATAGAAG GAATTGCTGA ATCTATTGGA 
AAAACCCAAT TCAGTTCTGA AAATTCCCAT ATGAACCTTG GTCACAGGTC TCTGGTTCAC 
AGCATGAACA CTGATTGCAA AGGAAATGAT AAAGAGTTAA ATGATTCAAA AGCTTCTCAC 
ACAGATAACT CAAGAATGGG CTCCTCCATA GGAAAAAAGC CAAGTCTGGC TTCAGAATCC 
AGTATTCATG CTACAATTAC TCCCTCAGTT GTTAGTTTCA CTAGTTTATT TAGCAGTAAA 
CCCTTTCTGA AGATTGGTGC AGTAACTGCA TCAGAGAAAC ACTGCCAAGT TGCTGAAAGC 
CTGAGCACTA GTTTGCAGTC CAAACCATTA AAAAAAAGAA AAGGAAGGAA ACCACGATGG 
ACTAAAGTGG TAGCAAGAAA CACATGCCGG CCTTCAAAAG GGCTAGAATT AGAAAGGTCA 
GAGCTTTTTA AAAATGTTTC ATGTAGCTCT TTGTCAAATA GCAATTCCGA GCCAGCCAAG 
TTTATGAAAA ACATTGGGCC ATCTTCATTT GTGGAGCATG ACTTCCTTAA ATGCCGATTG 
CCAAAGCTGA GTAAATCTAG CACAGCTCCA TCTCTTGCTC TCTTAACTGA AACTGACAAG 
CAGTCTCATA AGTCCTTTGC CAGCCACAAA CTTTCCTCTA GTATATGCAT GTCTGGTGAC 
CTTTTACCTG ACATTTATAA ACCTAAAAGA GGAAGACCTA AAACTAAGGA GATGCCCCAA 
CTAGAAGGTC CACCCAAAAG GACCCTAAAA ATCCCGGCCT CTAAAGTTTT TTCATTACAG 
TCAAAAGAGG AGCAAGAACC CCCTATTTTG CAGCCAGAAA TTGAAATCCC TTCCTTCAAG 
CAGAGCCTAT CAGTATCACC CTTTCCCAAA AAAAGAGGAA GGCCAAAAAG ACAAATCAGA 
TCACCAGTCA AGATGAAACC ACCTGTGCTA TCAGTGGCAC CCTTTGTAGC CACTGAAAGT 
CCAAGTAAAC TAGAGTCTGA GAGTGATAAA CATAGAAGTA GTAGTGACTT CTTTGAAAGG 
AAAGATCAAC TTCAGGGTCC AGATGACAGT CATAAACCAA GTGTGTGTGG TATGAGTGAC 
CTTGAGATGG AACCTGATAA AAAAATTAAT AAGAGAAACA ATGGCCAATT AATGAAAACT 
ATCATTCGCA AAATAAATAA GATGAAGACT TTGAAGAGAA AGAAACTGCT GAATCAGATT 
CTCTCAAGTT CTGTTGAATC AAGTAACAAA GGGAAAGTGC AATCCAAACT CCACAATACA 
GTGTCAACAC TTGCTGCCAC TTTTGGCTCC AAACTGGGCC AACAGATAAA TGTCAGCAAG 
AAAGGAACCA TTTATATAGG AAAGAGGCGA GGCCGGAAAC CAAAAGCTGT GCTGAACGGC 
ATCTTAACTA GTAGCCCAGC TAGTCTGGCT GTCCTAGAGA AAACAGCTCA TCAGGTAGCT 
GGGTCAGCAT TAGGACAGAT TCTCCCCCCA CTCTTGCCTT CAGCAGCAAG TAGTTCTGAG 
GTTCTTCCAT CCCCCATCTG TTCTCAGTCT TCTGGGACTA GTGGGGGCCA GAGTCCTGTC 
AGCAGTGATG CAGGATTTGT TGAGCCCAGT TCAGTGCCAT ATTTACATAT ACACTCCAGA 
CAGGGCAGTA TGATTCAGAC ACTTGCTATG AAGAAGGCTT CAAAGGGAAG GAGACGGTTG 
TCTCCTCCTA CTTTGTTGCC AAATTCTCCT TCTCACTTGA GTGAACTCAC TTCTCTGAAG 
GAAGCTACTC CTTCCCCAAT TAGTGAATCT CATAGTGATG AGACCATTCC TAGTGATAGT 
GGAATTGGAA CAGATAATAA TAGCACATCA GACAGGGCAG AAAAGTTTTG TGGGCAGAAA 
AAGAGGAGGC ATTCTTTTGA ACATGTTTCT CTCATTCCCC CTGAAACCTC CACAGTCCTA 
AGTAGTCTCA AAGAGAAACA TAAACACAAA TGTAAACGCA GGAGTCATGA TTACCTCAGT 
TATGACAAAA TGAAAAGACA GAAGCGAAAA CGGAAAAAGA AGTATCCCCA GTTACGAAAC 
AGACAGGATC CAGACTTTCT TGCTGAGCTG GAGGAATTAA TAAGTCGTTT AAGTGAAATT 
CGGATCACTC ATCGAAGTCA TCATTTTATT CCCCGTGATC TCCTACCCAC TATTTTTCGA 
ATTAACTTTA ACAGTTTCTA TACGCACCCC ACTTTTCCCT TAGACCCTTT GCACTACATT 
AGAAAACCTG ACTTAAAGAA AAAAAGAGGG AGGCCTCCCA AGATGAGGGA AGCAATGGCT 
GAAATGCCTT TTATGCATAG CCTTAGTTTC CCTCTCTCTA GTACTGGGTT TTACCCCTCT 
TATGGCATGC CTTATTCTCC CTCTCCACTT GCAGCTGCTC CCATAGGATT AGGATACTAT 
GGAAGGTACC CTCCAACTCT TTATCCACCA CCCCCCTCTC CTTCTTTCAC CACTCCTTTG 
CCACCTCCTT CTTACATGCA TGCTGGTCAT TTGCTTCTCA ATCCTGCCAA GTACCACAAG 
AAGAAACATA AGCTGCTTCG TCAGGAAGCC TTTCTTACAA CAAGTAGGAC ACCCCTTCTC 
TCCATGAGCA CCTACCCCAG TGTCCCTCCT GAGATGGCCT ATGGTTGGAT GGTGGAGCAT 
AAACACCGGC ACCGGCACAA ACACAGAGAA CACCGTTCTT CTGAGCAACC CCAGGTTTCC 
ATGGACACTT TAGCTACCAA TAGCTCTTCC AGAACTGTCC TAGAATCGTT GAAGCGCTAT 
CGATTTGGTA AAGATGCTGT TGGGGAGCGA TATAAACACA AAGAGAAGCA TCGTTGTCAC 
ATGTCCTGTC CTCATCTGTC CCCATCAAAA AGCTTATTAA GCCGAGAGGA GCAATGGGTC 
CACCGAGAGC CTTCAGAATC CACTTCATTG GCCTTGGGGT TGCAGACACC TTTACAGATT 
GACTGTTCAG AAACGTCTCC AGGTTTGTCC CTGGGAGGTT TTACACCTAG CTCTGAGCCA 
GCCAGCAGTG ATGAACACAC AAACCTTTTC ACAAGTGCAA TAGGCAGCTG CAGAGTATCA 
AACTCTACTA ATACAAGTGG CCGGAAGAAA TTGACTGACA GTCCAGGACT CTTTACCGCA 
CAGGACACTT CATTAAATCG GCCTCTTAGA AAGGAAACTT TGCCTTCCAA TGAAAGGGCA 
CTGCAAACTT TGACAGGCTC CCAGCCAGTC CTAGACAAGC CTTCCCAAAG GCCATCAGAG 
AGCACAAACT GTAGCCCTAC TCGAAAGAGA TCTTCATCAG AGAGCACTTC TTCAACAGTG 
ATTGGAATCC CTTCCCGAGG TACAAGATTA GGAACATCTG TGGATGACTC TGTGGATAGC 
CTGCTACAGC GTATGGCGCA ACATGAAAGC CAAGGGACCT TAGAGAAAAA CATTGATGCT 
GTCATTGCCA CAGTGTCCAT CCCACCTTCC TCAAGCCCAG GTCATACTCA TGGTAGGGAA 
CGAAGTCTGG GGAAATCAGA CAGTCTGCTA GTACCCACAG CACCTAGTGA CTCCTGCAGC 
AACACCACCA CCACCATCTC ACTCCTTTCT GAGAGGTTGC CAAGCAGCTG TTCCCCCCAT 
CATCTCAAAA GGAGTGTGGT AGAAGCCATG CAACGCCAAG CCCGGAAAAT GTGCAATTAT 
AACAAAATCT TGGCCACCAA GAAAAACCTA GACCATGTCA ATAAAATCTT AAAAGCCAAA 
AAGCTTCAAA GACAGGCAAG GACAGGTAAC AATTTTGTGA AGCGCAGACC AGGCCGACCC 
CGGAAATATC CCCTCCAGGC TGTGGTGTCA ATGCAGGCAT TCCAGGCTGC CCAATTTGCC 
AGCCCAGAAT TAGATGAAGG GGAGGAAGGC AGAGCACTAC ATCTTGGTCC TGATACAGTT 
ACAGATGTCA TTGAGGCTGT TGTTCAGAGT GTGAACCTGA ACCCAGAACT TAGAAAAGGA 
TGGAAAAGGA AAAGTTGGCT TCTGGAGGAA GAGGCAAGGA AAAGACAGAA GATACTTCCA 
GAGGAAGAAC AGGAAAATAA TAAAAGCTTT CCTGAGACAA CAATTGAATC TCCTAGTCCT 
CCAGAAGCCC CACCAAAGCC ACCTGAACCT GAAAATACCC TGCAGCCTTT GCCTTCTCTT 
ATCCCTCGGG AAAAGAAGGC CCCACGTCCC CCAAAGAAGA AATATCAGAA AGCAGGGCTC 
TATTCTGATG TATACAAAAC TACAGATCCA AAGAGCAGAC TGATACAATT AAAGAAAGAA 
AAACTGGAGT ATACTCCTGG AGAACATGAA TATGGATTAT TCCCAGCCCC TATTCATGTT 
GGAAAGTATT TGAGACAAAA GAGAATTGAC TTTCAGCTTC CTTATGACAT CCTCTGGCAA 
TGGAAACACA ATCAGCTATA TAAAAAGCCA GATGTCCCAC TATACAAAAA AATCCGTTCA 
AATGTCTACG TTGATGTCAA ACCTCTTTCT GGCTATGAGG CTACCACCTG CAACTGTAAA 
AAACCAGATG ATGGTGATAG GAAGGGTTGT ATGGATGACT GCCTCAACAG AATGATCTTT 
GCTGAGTGTT CCCCCAATAC CTGCCCCTGT GGAGAGCAGT GCTGCAATCA GAGGATACAA 
AGGCATGAGT GGGTACAGTG TCTTGAGCGG TTCCGAGCTG AGGAGAAGGG ATGGGGGATC 
CGGACCAAAG AACCTTTGAA AGCTGGCCAG TTCATTATTG AATACTTGGG AGAAGTTGTC 
AGTGAGCAGG AGTTCAGAAA CCGAATGATT GAACAGTACC ATAATCACAG TGACCATTAC 
TGCCTAAATC TGGACAGTGG AATGGTGATT GACAGCTACC GAATGGGCAA TGAGGCCCGG 
TTCATCAACC ACAGCTGTAA CCCAAATTGT GAAATGCAGA AATGGTCTGT TAATGGAGTC 
TACCGGATTG GGCTCTATGC CCTGAAAGAT ATGCCAGCTG GTACTGAACT AACCTATGAC 
TACAATTTTC ATTCTTTTAA TGTTGAGAAA CAGCAACTTT GTAAGTGTGG TTTTGACAAA 
TGCCGGGGTA TCATCGGAGG CAAGAGCCAA CGCATGAATG GACTCACCAG CAAAAGCAGC 
CAACCTGTGA CCACACACAG ACGGCCAGGA CGGTCAAAAG AGAAGAGAAA ATCCAAGCAC 
AAGTTGAAGA AAAGGAGAGG CCATCTTTCT GAGGAGCCTA ATGAAAATGT CAGTACCCCA 
ACCAGAATGA CCCCCCAACT GCAAATGAAG CCAATGTCCA ACCGTGAAAG GAACTTTGTG 
TTAAAACATC ATGTATTCCT GGTCCGGAAC TGGGAGAAAA TCAGACAGAA GCAAGAGGAG 
GTAAAACACA CCAGTGACAA TATCCATTCA GCTTCCTTAT ATACCCGCTG GAATGGTATC 
TGCCGAGATG ATGGAAACAT CAAATCTGAT GTCTTCATGA CCCAGTTCTC CGCCTTGCAG 
ACAGCCCGCT CAGTTCGTAC AAGGAGATTG GCAGCTGCAG AGGAGAACAT TGAAGTGGCC 
CGGGCAGCCC GCCTGGCCCA GATATTCAAA GAGATATGTG ACAGCATCAT CTCCTACAAA 
GATTCCTCCC GACAGGCATT GGCAACTCCA CTGTTGAACC TTCCCCCAAA GAAAAAGAAT 
GCTGATTATT ATGAGAAGAT ATCTGACCCC TTAGATCTCA TTACCATTGA GAAGCAGATC 
CTGACAGGGT ATTACAAGAC AGTGGAGGCG TTTGATGCTG ACATGCTCAA GGTCTTCCGG 
AATGCTGAGA AATACTATGG ACGCAAGTCC CCAATTGGGA GAGATGTATG TCGACTACGC 
AAAGCATACT ACAATGCACG GCATGAGGCA TCAGCTCAAA TTGATGAGAT TGTGGGGGAA 
ACAGCAAGTG AAGCAGACAG CAGCGAAACC TCAGTCTGTG AGAAGGAAAA TGGGCATGAG 
AAGGATGATG ATGTTATCCG CTGTATCTGC GGCCTCTACA AGGATGAAGG CCTCATGATT 
CAGTGTGACA AGTGCATGGT GTGGCAGCAC TGTGATTGCA TGGGAGTAAA CTCAGATGTA 
GAACATTACC TCTGTGAACA ATGTGATCCA AGGCCTGTGG ACAGGGAAGT GCCCATGATA 
CCTCGGCCCC ATTATGCCCA ACCTGGCTGT GTCTACTTCA TCTGTCTACT CCGGGATGAC 
CTGCTGCTAC GTCAAGGTGA CTGTGTGTAC CTGATGCGAG ATAGTCGGAG AACCCCTGAT 
GGCCACCCAG TCCGTCAGTC TTATCGATTG TTATCCCATA TCAACCGAGA GAAACTTGAT 
ATCTTTCGAA TTGAGAAGCT TTGGAAGAAT GAAAAAGAGG AGCGGTTTGC CTTTGGTCAT 
CATTATTTTC GTCCCCATGA AACCCACCAC TCCCCATCAC GTCGATTTTA TCACAATGAG 
CTGTTCCGAG TGCCACTGTA TGAAATCATT CCTCTGGAGG CTGTGGTGGG GACCTGTTGC 
GTATTAGATC TGTACACATA TTGTAAAGGA AGGCCCAAAG GAGTGAAAGA GCAGGATGTA 
TATATCTGTG ACTACCGACT TGATAAATCA GCCCACCTGT TCTACAAGAT TCATCGGAAC 
CGCTATCCTG TCTGCACCAA ACCCTATGCC TTTGACCACT TCCCCAAGAA GCTCACTCCC 
AAGAGAGATT TCTCACCTCA TTATGTCCCA GACAACTACA AGAGGAATGG AGGAAGATCA 
TCCTGGAAGA CAGAACGTTC AAAGCCACCC ATCAAAGACC TAGGCCAGGA GGATGATGCC 
CTACCACTCA TTGAAGAAGT ACTGGCCAGT CAAGAGCAAG CAGCCAATGA GATGCCCACC 
CTAGAAGAAA CTGATCGGGA GGTAGCACCT GGTGAAACAA CTGAGGGTGA AAAGAAAGCT 
GATGAAAGCA GACAGGAGCC TCAGAGGTCC TGCACACCTG AAGAGAGGAG ACACAGCCAA 
CGGGAGCGAC TCAACCAGAT TCTGCTCAAC CTTCTTGAGA AGATCCCTGG GAAGAATGCC 
ATCGATGTGA CATACTTGCT GGAGGAAGGA TCAGGAAGAA AGCTTCGAAG ACGTACTCTG 
CTTATCCCAG AGAGCAGCTT CCGAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASH1L ( XM_003768076.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003768124.1 Sarcophilus harrisii ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), mRNA.

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CloneID	OSa130098
Clone ID Related Accession (Same CDS sequence)	XM_003768076.3 , XM_031966723.1 , XM_003768076.2
Accession Version	XM_003768076.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	histone-lysine N-methyltransferase ASH1L
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838832.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 15, 2015 this sequence version replaced XM_003768076.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATA GGAATACTGC TATGTTAGGA GTGGGTACCG ACTCCGAAGG ATTTTCAAGG 
AAGAGTCCTT CTGCTGTCAG CACTGGAACA CTGGTTAGTA AGAGAGAAGT GGAACTAGAG 
AATAGCACTA AGGAAGAAGA GGATCTTTGG AGGCGGAATA GGGACAGAAA CATTGAGACA 
GGGAAAAATG ATGGTTTGAC TGATGCACAG CAGTTTTCTG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAGTTGAA AATTGGCCTT CAGGCTAAGA GAACTAAAAA GCCTCCTAAA 
AACTTGGAGA ACTATGTATG TCGACCTGCT ATAAAAACAA GCATTAAGCA TCCAAGGAAA 
GCATTGAAAA GTGGGAAGAT GACAGATGAA AAAAATGAAC TTTGCCCTTC AAAACGAGAC 
CCTTCAAAAC TTTACAAGAA AGCAAATGAT GTTGCAGCTG TTGAATGCCG CTCTGAAGAA 
GGCATCCACC TTCATTCACA GGGAGAAAAT AATCCTGTGT CAAAGAAGCT TTCTCCACTA 
CACTCTGAAA TGACAGATTA TGTTAAATCA GCACCATCTA CTCTTGTTGG TAACCGTGAT 
CCTGACTCGA AGGACAGAGC TCTACTTAAT GGAGCAACAA GTGTAACAGA AAAATTGGCA 
CAGCTCATTG CTACTTGTCC ACCCTCCAAG TCTTCCAAGA CAAAGCCAAA AAAGTTAGGG 
GCTGGTATTA CAGCAGGATT GGTTAGCAAA GACTTGATCA GAAAGCCAGG CATTGGTCCT 
ATGGCAGGAA TTATTCACAA GGACTTGATT AAGAAACCAA CAATTAGCCC AGCAGTTGGG 
TTGGTAACTA AAGACCCTGG GAAAAAGCCA ATCTTTAGTG CTGCAGTAGG ATTGGTCAGT 
AAAGACTCTG GGAAAAAATT AGGAACTGGG ACTACTGCGG TATTCATAAA CAAGGACTTA 
GGAAAGAAAC CAGGGACTAT CACTACAGTG GGACTGGTGA GCAGAGAGTC AGGGAAGAAA 
TTAGGAATTG GTATCGTCCC AGGTTTGGTG AGTAAAGAAT CAGGAAAGAA GTTGGGACTT 
GGTACTGTAG TTGGGCTTGT TAATAAAGAT TCAGTAAAGA AGTTTGGCCC TGCCATTGGA 
CTGGTTGCAA AGGACTGTGG GAAGAAGACT ATAACAAATT CATCAGTGGG ATTGGTTAAT 
AAGGACCCTG CAAAGAAATT GGTGGCTTGC CCTGTAGCAG GATTGGTCAA CAAAGACATT 
GTGAATTTAA AAGCTGAAGC CTTGCTCCCT GTTCAAGAAC CACTTAAGGT TTCTTGCAAT 
ACAAATACTA GTAGTCATGA AAGTCAGGAG CATTCTGAAT CCCTGAAAGA TAGTGCCACC 
AGCAAAGCTT TTGAGAAGAC TATTATTCGT CAGAATAAAG ATAGTTTATT AGAAAAGTTC 
TCAATTCGAA AAGAAATCAT TACTTTGGAC AAAGACATTT TTAATGAAGG AACTTGCATA 
CAGCAGGACA GTTTCTTATC CAGTGAAAGG GGGACCTATG TTGAAACTAC AAAGCATGAG 
AAGCAGCCTC CAGTGTATTG CACTTCACCA GATTTTCAAA TGGGAGGCAC TTCTGATGCA 
TCAACAGCTA AGTCTCCCTT TAGTGCAATA GGGGAGAGTA ATCTCCCTTC CCCTTCACCA 
GCCTTGTCTG TTACGCCCTT AAACAGAAGT CCCCCTGGAA CTTCTTCTCA GTTGGCTACT 
AATCCATTAC ATTTAGGTCC TTCCACAGAA CTCATAGAAG GAATTGCTGA ATCTATTGGA 
AAAACCCAAT TCAGTTCTGA AAATTCCCAT ATGAACCTTG GTCACAGGTC TCTGGTTCAC 
AGCATGAACA CTGATTGCAA AGGAAATGAT AAAGAGTTAA ATGATTCAAA AGCTTCTCAC 
ACAGATAACT CAAGAATGGG CTCCTCCATA GGAAAAAAGC CAAGTCTGGC TTCAGAATCC 
AGTATTCATG CTACAATTAC TCCCTCAGTT GTTAGTTTCA CTAGTTTATT TAGCAGTAAA 
CCCTTTCTGA AGATTGGTGC AGTAACTGCA TCAGAGAAAC ACTGCCAAGT TGCTGAAAGC 
CTGAGCACTA GTTTGCAGTC CAAACCATTA AAAAAAAGAA AAGGAAGGAA ACCACGATGG 
ACTAAAGTGG TAGCAAGAAA CACATGCCGG CCTTCAAAAG GGCTAGAATT AGAAAGGTCA 
GAGCTTTTTA AAAATGTTTC ATGTAGCTCT TTGTCAAATA GCAATTCCGA GCCAGCCAAG 
TTTATGAAAA ACATTGGGCC ATCTTCATTT GTGGAGCATG ACTTCCTTAA ATGCCGATTG 
CCAAAGCTGA GTAAATCTAG CACAGCTCCA TCTCTTGCTC TCTTAACTGA AACTGACAAG 
CAGTCTCATA AGTCCTTTGC CAGCCACAAA CTTTCCTCTA GTATATGCAT GTCTGGTGAC 
CTTTTACCTG ACATTTATAA ACCTAAAAGA GGAAGACCTA AAACTAAGGA GATGCCCCAA 
CTAGAAGGTC CACCCAAAAG GACCCTAAAA ATCCCGGCCT CTAAAGTTTT TTCATTACAG 
TCAAAAGAGG AGCAAGAACC CCCTATTTTG CAGCCAGAAA TTGAAATCCC TTCCTTCAAG 
CAGAGCCTAT CAGTATCACC CTTTCCCAAA AAAAGAGGAA GGCCAAAAAG ACAAATCAGA 
TCACCAGTCA AGATGAAACC ACCTGTGCTA TCAGTGGCAC CCTTTGTAGC CACTGAAAGT 
CCAAGTAAAC TAGAGTCTGA GAGTGATAAA CATAGAAGTA GTAGTGACTT CTTTGAAAGG 
AAAGATCAAC TTCAGGGTCC AGATGACAGT CATAAACCAA GTGTGTGTGG TATGAGTGAC 
CTTGAGATGG AACCTGATAA AAAAATTAAT AAGAGAAACA ATGGCCAATT AATGAAAACT 
ATCATTCGCA AAATAAATAA GATGAAGACT TTGAAGAGAA AGAAACTGCT GAATCAGATT 
CTCTCAAGTT CTGTTGAATC AAGTAACAAA GGGAAAGTGC AATCCAAACT CCACAATACA 
GTGTCAACAC TTGCTGCCAC TTTTGGCTCC AAACTGGGCC AACAGATAAA TGTCAGCAAG 
AAAGGAACCA TTTATATAGG AAAGAGGCGA GGCCGGAAAC CAAAAGCTGT GCTGAACGGC 
ATCTTAACTA GTAGCCCAGC TAGTCTGGCT GTCCTAGAGA AAACAGCTCA TCAGGTAGCT 
GGGTCAGCAT TAGGACAGAT TCTCCCCCCA CTCTTGCCTT CAGCAGCAAG TAGTTCTGAG 
GTTCTTCCAT CCCCCATCTG TTCTCAGTCT TCTGGGACTA GTGGGGGCCA GAGTCCTGTC 
AGCAGTGATG CAGGATTTGT TGAGCCCAGT TCAGTGCCAT ATTTACATAT ACACTCCAGA 
CAGGGCAGTA TGATTCAGAC ACTTGCTATG AAGAAGGCTT CAAAGGGAAG GAGACGGTTG 
TCTCCTCCTA CTTTGTTGCC AAATTCTCCT TCTCACTTGA GTGAACTCAC TTCTCTGAAG 
GAAGCTACTC CTTCCCCAAT TAGTGAATCT CATAGTGATG AGACCATTCC TAGTGATAGT 
GGAATTGGAA CAGATAATAA TAGCACATCA GACAGGGCAG AAAAGTTTTG TGGGCAGAAA 
AAGAGGAGGC ATTCTTTTGA ACATGTTTCT CTCATTCCCC CTGAAACCTC CACAGTCCTA 
AGTAGTCTCA AAGAGAAACA TAAACACAAA TGTAAACGCA GGAGTCATGA TTACCTCAGT 
TATGACAAAA TGAAAAGACA GAAGCGAAAA CGGAAAAAGA AGTATCCCCA GTTACGAAAC 
AGACAGGATC CAGACTTTCT TGCTGAGCTG GAGGAATTAA TAAGTCGTTT AAGTGAAATT 
CGGATCACTC ATCGAAGTCA TCATTTTATT CCCCGTGATC TCCTACCCAC TATTTTTCGA 
ATTAACTTTA ACAGTTTCTA TACGCACCCC ACTTTTCCCT TAGACCCTTT GCACTACATT 
AGAAAACCTG ACTTAAAGAA AAAAAGAGGG AGGCCTCCCA AGATGAGGGA AGCAATGGCT 
GAAATGCCTT TTATGCATAG CCTTAGTTTC CCTCTCTCTA GTACTGGGTT TTACCCCTCT 
TATGGCATGC CTTATTCTCC CTCTCCACTT GCAGCTGCTC CCATAGGATT AGGATACTAT 
GGAAGGTACC CTCCAACTCT TTATCCACCA CCCCCCTCTC CTTCTTTCAC CACTCCTTTG 
CCACCTCCTT CTTACATGCA TGCTGGTCAT TTGCTTCTCA ATCCTGCCAA GTACCACAAG 
AAGAAACATA AGCTGCTTCG TCAGGAAGCC TTTCTTACAA CAAGTAGGAC ACCCCTTCTC 
TCCATGAGCA CCTACCCCAG TGTCCCTCCT GAGATGGCCT ATGGTTGGAT GGTGGAGCAT 
AAACACCGGC ACCGGCACAA ACACAGAGAA CACCGTTCTT CTGAGCAACC CCAGGTTTCC 
ATGGACACTT TAGCTACCAA TAGCTCTTCC AGAACTGTCC TAGAATCGTT GAAGCGCTAT 
CGATTTGGTA AAGATGCTGT TGGGGAGCGA TATAAACACA AAGAGAAGCA TCGTTGTCAC 
ATGTCCTGTC CTCATCTGTC CCCATCAAAA AGCTTATTAA GCCGAGAGGA GCAATGGGTC 
CACCGAGAGC CTTCAGAATC CACTTCATTG GCCTTGGGGT TGCAGACACC TTTACAGATT 
GACTGTTCAG AAACGTCTCC AGGTTTGTCC CTGGGAGGTT TTACACCTAG CTCTGAGCCA 
GCCAGCAGTG ATGAACACAC AAACCTTTTC ACAAGTGCAA TAGGCAGCTG CAGAGTATCA 
AACTCTACTA ATACAAGTGG CCGGAAGAAA TTGACTGACA GTCCAGGACT CTTTACCGCA 
CAGGACACTT CATTAAATCG GCCTCTTAGA AAGGAAACTT TGCCTTCCAA TGAAAGGGCA 
CTGCAAACTT TGACAGGCTC CCAGCCAGTC CTAGACAAGC CTTCCCAAAG GCCATCAGAG 
AGCACAAACT GTAGCCCTAC TCGAAAGAGA TCTTCATCAG AGAGCACTTC TTCAACAGTG 
ATTGGAATCC CTTCCCGAGG TACAAGATTA GGAACATCTG TGGATGACTC TGTGGATAGC 
CTGCTACAGC GTATGGCGCA ACATGAAAGC CAAGGGACCT TAGAGAAAAA CATTGATGCT 
GTCATTGCCA CAGTGTCCAT CCCACCTTCC TCAAGCCCAG GTCATACTCA TGGTAGGGAA 
CGAAGTCTGG GGAAATCAGA CAGTCTGCTA GTACCCACAG CACCTAGTGA CTCCTGCAGC 
AACACCACCA CCACCATCTC ACTCCTTTCT GAGAGGTTGC CAAGCAGCTG TTCCCCCCAT 
CATCTCAAAA GGAGTGTGGT AGAAGCCATG CAACGCCAAG CCCGGAAAAT GTGCAATTAT 
AACAAAATCT TGGCCACCAA GAAAAACCTA GACCATGTCA ATAAAATCTT AAAAGCCAAA 
AAGCTTCAAA GACAGGCAAG GACAGGTAAC AATTTTGTGA AGCGCAGACC AGGCCGACCC 
CGGAAATATC CCCTCCAGGC TGTGGTGTCA ATGCAGGCAT TCCAGGCTGC CCAATTTGCC 
AGCCCAGAAT TAGATGAAGG GGAGGAAGGC AGAGCACTAC ATCTTGGTCC TGATACAGTT 
ACAGATGTCA TTGAGGCTGT TGTTCAGAGT GTGAACCTGA ACCCAGAACT TAGAAAAGGA 
TGGAAAAGGA AAAGTTGGCT TCTGGAGGAA GAGGCAAGGA AAAGACAGAA GATACTTCCA 
GAGGAAGAAC AGGAAAATAA TAAAAGCTTT CCTGAGACAA CAATTGAATC TCCTAGTCCT 
CCAGAAGCCC CACCAAAGCC ACCTGAACCT GAAAATACCC TGCAGCCTTT GCCTTCTCTT 
ATCCCTCGGG AAAAGAAGGC CCCACGTCCC CCAAAGAAGA AATATCAGAA AGCAGGGCTC 
TATTCTGATG TATACAAAAC TACAGATCCA AAGAGCAGAC TGATACAATT AAAGAAAGAA 
AAACTGGAGT ATACTCCTGG AGAACATGAA TATGGATTAT TCCCAGCCCC TATTCATGTT 
GGAAAGTATT TGAGACAAAA GAGAATTGAC TTTCAGCTTC CTTATGACAT CCTCTGGCAA 
TGGAAACACA ATCAGCTATA TAAAAAGCCA GATGTCCCAC TATACAAAAA AATCCGTTCA 
AATGTCTACG TTGATGTCAA ACCTCTTTCT GGCTATGAGG CTACCACCTG CAACTGTAAA 
AAACCAGATG ATGGTGATAG GAAGGGTTGT ATGGATGACT GCCTCAACAG AATGATCTTT 
GCTGAGTGTT CCCCCAATAC CTGCCCCTGT GGAGAGCAGT GCTGCAATCA GAGGATACAA 
AGGCATGAGT GGGTACAGTG TCTTGAGCGG TTCCGAGCTG AGGAGAAGGG ATGGGGGATC 
CGGACCAAAG AACCTTTGAA AGCTGGCCAG TTCATTATTG AATACTTGGG AGAAGTTGTC 
AGTGAGCAGG AGTTCAGAAA CCGAATGATT GAACAGTACC ATAATCACAG TGACCATTAC 
TGCCTAAATC TGGACAGTGG AATGGTGATT GACAGCTACC GAATGGGCAA TGAGGCCCGG 
TTCATCAACC ACAGCTGTAA CCCAAATTGT GAAATGCAGA AATGGTCTGT TAATGGAGTC 
TACCGGATTG GGCTCTATGC CCTGAAAGAT ATGCCAGCTG GTACTGAACT AACCTATGAC 
TACAATTTTC ATTCTTTTAA TGTTGAGAAA CAGCAACTTT GTAAGTGTGG TTTTGACAAA 
TGCCGGGGTA TCATCGGAGG CAAGAGCCAA CGCATGAATG GACTCACCAG CAAAAGCAGC 
CAACCTGTGA CCACACACAG ACGGCCAGGA CGGTCAAAAG AGAAGAGAAA ATCCAAGCAC 
AAGTTGAAGA AAAGGAGAGG CCATCTTTCT GAGGAGCCTA ATGAAAATGT CAGTACCCCA 
ACCAGAATGA CCCCCCAACT GCAAATGAAG CCAATGTCCA ACCGTGAAAG GAACTTTGTG 
TTAAAACATC ATGTATTCCT GGTCCGGAAC TGGGAGAAAA TCAGACAGAA GCAAGAGGAG 
GTAAAACACA CCAGTGACAA TATCCATTCA GCTTCCTTAT ATACCCGCTG GAATGGTATC 
TGCCGAGATG ATGGAAACAT CAAATCTGAT GTCTTCATGA CCCAGTTCTC CGCCTTGCAG 
ACAGCCCGCT CAGTTCGTAC AAGGAGATTG GCAGCTGCAG AGGAGAACAT TGAAGTGGCC 
CGGGCAGCCC GCCTGGCCCA GATATTCAAA GAGATATGTG ACAGCATCAT CTCCTACAAA 
GATTCCTCCC GACAGGCATT GGCAACTCCA CTGTTGAACC TTCCCCCAAA GAAAAAGAAT 
GCTGATTATT ATGAGAAGAT ATCTGACCCC TTAGATCTCA TTACCATTGA GAAGCAGATC 
CTGACAGGGT ATTACAAGAC AGTGGAGGCG TTTGATGCTG ACATGCTCAA GGTCTTCCGG 
AATGCTGAGA AATACTATGG ACGCAAGTCC CCAATTGGGA GAGATGTATG TCGACTACGC 
AAAGCATACT ACAATGCACG GCATGAGGCA TCAGCTCAAA TTGATGAGAT TGTGGGGGAA 
ACAGCAAGTG AAGCAGACAG CAGCGAAACC TCAGTCTGTG AGAAGGAAAA TGGGCATGAG 
AAGGATGATG ATGTTATCCG CTGTATCTGC GGCCTCTACA AGGATGAAGG CCTCATGATT 
CAGTGTGACA AGTGCATGGT GTGGCAGCAC TGTGATTGCA TGGGAGTAAA CTCAGATGTA 
GAACATTACC TCTGTGAACA ATGTGATCCA AGGCCTGTGG ACAGGGAAGT GCCCATGATA 
CCTCGGCCCC ATTATGCCCA ACCTGGCTGT GTCTACTTCA TCTGTCTACT CCGGGATGAC 
CTGCTGCTAC GTCAAGGTGA CTGTGTGTAC CTGATGCGAG ATAGTCGGAG AACCCCTGAT 
GGCCACCCAG TCCGTCAGTC TTATCGATTG TTATCCCATA TCAACCGAGA GAAACTTGAT 
ATCTTTCGAA TTGAGAAGCT TTGGAAGAAT GAAAAAGAGG AGCGGTTTGC CTTTGGTCAT 
CATTATTTTC GTCCCCATGA AACCCACCAC TCCCCATCAC GTCGATTTTA TCACAATGAG 
CTGTTCCGAG TGCCACTGTA TGAAATCATT CCTCTGGAGG CTGTGGTGGG GACCTGTTGC 
GTATTAGATC TGTACACATA TTGTAAAGGA AGGCCCAAAG GAGTGAAAGA GCAGGATGTA 
TATATCTGTG ACTACCGACT TGATAAATCA GCCCACCTGT TCTACAAGAT TCATCGGAAC 
CGCTATCCTG TCTGCACCAA ACCCTATGCC TTTGACCACT TCCCCAAGAA GCTCACTCCC 
AAGAGAGATT TCTCACCTCA TTATGTCCCA GACAACTACA AGAGGAATGG AGGAAGATCA 
TCCTGGAAGA CAGAACGTTC AAAGCCACCC ATCAAAGACC TAGGCCAGGA GGATGATGCC 
CTACCACTCA TTGAAGAAGT ACTGGCCAGT CAAGAGCAAG CAGCCAATGA GATGCCCACC 
CTAGAAGAAA CTGATCGGGA GGTAGCACCT GGTGAAACAA CTGAGGGTGA AAAGAAAGCT 
GATGAAAGCA GACAGGAGCC TCAGAGGTCC TGCACACCTG AAGAGAGGAG ACACAGCCAA 
CGGGAGCGAC TCAACCAGAT TCTGCTCAAC CTTCTTGAGA AGATCCCTGG GAAGAATGCC 
ATCGATGTGA CATACTTGCT GGAGGAAGGA TCAGGAAGAA AGCTTCGAAG ACGTACTCTG 
CTTATCCCAG AGAGCAGCTT CCGAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003768124.1
CDS	85..8994
Translation	MDHRNTAMLGVGTDSEGFSRKSPSAVSTGTLVSKREVELENSTKEEEDLWRRNRDRNIETGKNDGLTDAQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTSIKHPRKALKSGKMTDEKNELCPSKRDPSKLYKKANDVAAVECRSEEGIHLHSQGENNPVSKKLSPLHSEMTDYVKSAPSTLVGNRDPDSKDRALLNGATSVTEKLAQLIATCPPSKSSKTKPKKLGAGITAGLVSKDLIRKPGIGPMAGIIHKDLIKKPTISPAVGLVTKDPGKKPIFSAAVGLVSKDSGKKLGTGTTAVFINKDLGKKPGTITTVGLVSRESGKKLGIGIVPGLVSKESGKKLGLGTVVGLVNKDSVKKFGPAIGLVAKDCGKKTITNSSVGLVNKDPAKKLVACPVAGLVNKDIVNLKAEALLPVQEPLKVSCNTNTSSHESQEHSESLKDSATSKAFEKTIIRQNKDSLLEKFSIRKEIITLDKDIFNEGTCIQQDSFLSSERGTYVETTKHEKQPPVYCTSPDFQMGGTSDASTAKSPFSAIGESNLPSPSPALSVTPLNRSPPGTSSQLATNPLHLGPSTELIEGIAESIGKTQFSSENSHMNLGHRSLVHSMNTDCKGNDKELNDSKASHTDNSRMGSSIGKKPSLASESSIHATITPSVVSFTSLFSSKPFLKIGAVTASEKHCQVAESLSTSLQSKPLKKRKGRKPRWTKVVARNTCRPSKGLELERSELFKNVSCSSLSNSNSEPAKFMKNIGPSSFVEHDFLKCRLPKLSKSSTAPSLALLTETDKQSHKSFASHKLSSSICMSGDLLPDIYKPKRGRPKTKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQIRSPVKMKPPVLSVAPFVATESPSKLESESDKHRSSSDFFERKDQLQGPDDSHKPSVCGMSDLEMEPDKKINKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSTLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKAVLNGILTSSPASLAVLEKTAHQVAGSALGQILPPLLPSAASSSEVLPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHIHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFLAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPTFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLAAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPAKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDTLATNSSSRTVLESLKRYRFGKDAVGERYKHKEKHRCHMSCPHLSPSKSLLSREEQWVHREPSESTSLALGLQTPLQIDCSETSPGLSLGGFTPSSEPASSDEHTNLFTSAIGSCRVSNSTNTSGRKKLTDSPGLFTAQDTSLNRPLRKETLPSNERALQTLTGSQPVLDKPSQRPSESTNCSPTRKRSSSESTSSTVIGIPSRGTRLGTSVDDSVDSLLQRMAQHESQGTLEKNIDAVIATVSIPPSSSPGHTHGRERSLGKSDSLLVPTAPSDSCSNTTTTISLLSERLPSSCSPHHLKRSVVEAMQRQARKMCNYNKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKYPLQAVVSMQAFQAAQFASPELDEGEEGRALHLGPDTVTDVIEAVVQSVNLNPELRKGWKRKSWLLEEEARKRQKILPEEEQENNKSFPETTIESPSPPEAPPKPPEPENTLQPLPSLIPREKKAPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPDDGDRKGCMDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCNPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFDKCRGIIGGKSQRMNGLTSKSSQPVTTHRRPGRSKEKRKSKHKLKKRRGHLSEEPNENVSTPTRMTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIRQKQEEVKHTSDNIHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENIEVARAARLAQIFKEICDSIISYKDSSRQALATPLLNLPPKKKNADYYEKISDPLDLITIEKQILTGYYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKAYYNARHEASAQIDEIVGETASEADSSETSVCEKENGHEKDDDVIRCICGLYKDEGLMIQCDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINREKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKTERSKPPIKDLGQEDDALPLIEEVLASQEQAANEMPTLEETDREVAPGETTEGEKKADESRQEPQRSCTPEERRHSQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLLIPESSFRK

Target ORF information:

RefSeq Version	XM_003768076.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003768076.2

ORF Insert Sequence:

ATGGACCATA GGAATACTGC TATGTTAGGA GTGGGTACCG ACTCCGAAGG ATTTTCAAGG 
AAGAGTCCTT CTGCTGTCAG CACTGGAACA CTGGTTAGTA AGAGAGAAGT GGAACTAGAG 
AATAGCACTA AGGAAGAAGA GGATCTTTGG AGGCGGAATA GGGACAGAAA CATTGAGACA 
GGGAAAAATG ATGGTTTGAC TGATGCACAG CAGTTTTCTG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAGTTGAA AATTGGCCTT CAGGCTAAGA GAACTAAAAA GCCTCCTAAA 
AACTTGGAGA ACTATGTATG TCGACCTGCT ATAAAAACAA GCATTAAGCA TCCAAGGAAA 
GCATTGAAAA GTGGGAAGAT GACAGATGAA AAAAATGAAC TTTGCCCTTC AAAACGAGAC 
CCTTCAAAAC TTTACAAGAA AGCAAATGAT GTTGCAGCTG TTGAATGCCG CTCTGAAGAA 
GGCATCCACC TTCATTCACA GGGAGAAAAT AATCCTGTGT CAAAGAAGCT TTCTCCACTA 
CACTCTGAAA TGACAGATTA TGTTAAATCA GCACCATCTA CTCTTGTTGG TAACCGTGAT 
CCTGACTCGA AGGACAGAGC TCTACTTAAT GGAGCAACAA GTGTAACAGA AAAATTGGCA 
CAGCTCATTG CTACTTGTCC ACCCTCCAAG TCTTCCAAGA CAAAGCCAAA AAAGTTAGGG 
GCTGGTATTA CAGCAGGATT GGTTAGCAAA GACTTGATCA GAAAGCCAGG CATTGGTCCT 
ATGGCAGGAA TTATTCACAA GGACTTGATT AAGAAACCAA CAATTAGCCC AGCAGTTGGG 
TTGGTAACTA AAGACCCTGG GAAAAAGCCA ATCTTTAGTG CTGCAGTAGG ATTGGTCAGT 
AAAGACTCTG GGAAAAAATT AGGAACTGGG ACTACTGCGG TATTCATAAA CAAGGACTTA 
GGAAAGAAAC CAGGGACTAT CACTACAGTG GGACTGGTGA GCAGAGAGTC AGGGAAGAAA 
TTAGGAATTG GTATCGTCCC AGGTTTGGTG AGTAAAGAAT CAGGAAAGAA GTTGGGACTT 
GGTACTGTAG TTGGGCTTGT TAATAAAGAT TCAGTAAAGA AGTTTGGCCC TGCCATTGGA 
CTGGTTGCAA AGGACTGTGG GAAGAAGACT ATAACAAATT CATCAGTGGG ATTGGTTAAT 
AAGGACCCTG CAAAGAAATT GGTGGCTTGC CCTGTAGCAG GATTGGTCAA CAAAGACATT 
GTGAATTTAA AAGCTGAAGC CTTGCTCCCT GTTCAAGAAC CACTTAAGGT TTCTTGCAAT 
ACAAATACTA GTAGTCATGA AAGTCAGGAG CATTCTGAAT CCCTGAAAGA TAGTGCCACC 
AGCAAAGCTT TTGAGAAGAC TATTATTCGT CAGAATAAAG ATAGTTTATT AGAAAAGTTC 
TCAATTCGAA AAGAAATCAT TACTTTGGAC AAAGACATTT TTAATGAAGG AACTTGCATA 
CAGCAGGACA GTTTCTTATC CAGTGAAAGG GGGACCTATG TTGAAACTAC AAAGCATGAG 
AAGCAGCCTC CAGTGTATTG CACTTCACCA GATTTTCAAA TGGGAGGCAC TTCTGATGCA 
TCAACAGCTA AGTCTCCCTT TAGTGCAATA GGGGAGAGTA ATCTCCCTTC CCCTTCACCA 
GCCTTGTCTG TTACGCCCTT AAACAGAAGT CCCCCTGGAA CTTCTTCTCA GTTGGCTACT 
AATCCATTAC ATTTAGGTCC TTCCACAGAA CTCATAGAAG GAATTGCTGA ATCTATTGGA 
AAAACCCAAT TCAGTTCTGA AAATTCCCAT ATGAACCTTG GTCACAGGTC TCTGGTTCAC 
AGCATGAACA CTGATTGCAA AGGAAATGAT AAAGAGTTAA ATGATTCAAA AGCTTCTCAC 
ACAGATAACT CAAGAATGGG CTCCTCCATA GGAAAAAAGC CAAGTCTGGC TTCAGAATCC 
AGTATTCATG CTACAATTAC TCCCTCAGTT GTTAGTTTCA CTAGTTTATT TAGCAGTAAA 
CCCTTTCTGA AGATTGGTGC AGTAACTGCA TCAGAGAAAC ACTGCCAAGT TGCTGAAAGC 
CTGAGCACTA GTTTGCAGTC CAAACCATTA AAAAAAAGAA AAGGAAGGAA ACCACGATGG 
ACTAAAGTGG TAGCAAGAAA CACATGCCGG CCTTCAAAAG GGCTAGAATT AGAAAGGTCA 
GAGCTTTTTA AAAATGTTTC ATGTAGCTCT TTGTCAAATA GCAATTCCGA GCCAGCCAAG 
TTTATGAAAA ACATTGGGCC ATCTTCATTT GTGGAGCATG ACTTCCTTAA ATGCCGATTG 
CCAAAGCTGA GTAAATCTAG CACAGCTCCA TCTCTTGCTC TCTTAACTGA AACTGACAAG 
CAGTCTCATA AGTCCTTTGC CAGCCACAAA CTTTCCTCTA GTATATGCAT GTCTGGTGAC 
CTTTTACCTG ACATTTATAA ACCTAAAAGA GGAAGACCTA AAACTAAGGA GATGCCCCAA 
CTAGAAGGTC CACCCAAAAG GACCCTAAAA ATCCCGGCCT CTAAAGTTTT TTCATTACAG 
TCAAAAGAGG AGCAAGAACC CCCTATTTTG CAGCCAGAAA TTGAAATCCC TTCCTTCAAG 
CAGAGCCTAT CAGTATCACC CTTTCCCAAA AAAAGAGGAA GGCCAAAAAG ACAAATCAGA 
TCACCAGTCA AGATGAAACC ACCTGTGCTA TCAGTGGCAC CCTTTGTAGC CACTGAAAGT 
CCAAGTAAAC TAGAGTCTGA GAGTGATAAA CATAGAAGTA GTAGTGACTT CTTTGAAAGG 
AAAGATCAAC TTCAGGGTCC AGATGACAGT CATAAACCAA GTGTGTGTGG TATGAGTGAC 
CTTGAGATGG AACCTGATAA AAAAATTAAT AAGAGAAACA ATGGCCAATT AATGAAAACT 
ATCATTCGCA AAATAAATAA GATGAAGACT TTGAAGAGAA AGAAACTGCT GAATCAGATT 
CTCTCAAGTT CTGTTGAATC AAGTAACAAA GGGAAAGTGC AATCCAAACT CCACAATACA 
GTGTCAACAC TTGCTGCCAC TTTTGGCTCC AAACTGGGCC AACAGATAAA TGTCAGCAAG 
AAAGGAACCA TTTATATAGG AAAGAGGCGA GGCCGGAAAC CAAAAGCTGT GCTGAACGGC 
ATCTTAACTA GTAGCCCAGC TAGTCTGGCT GTCCTAGAGA AAACAGCTCA TCAGGTAGCT 
GGGTCAGCAT TAGGACAGAT TCTCCCCCCA CTCTTGCCTT CAGCAGCAAG TAGTTCTGAG 
GTTCTTCCAT CCCCCATCTG TTCTCAGTCT TCTGGGACTA GTGGGGGCCA GAGTCCTGTC 
AGCAGTGATG CAGGATTTGT TGAGCCCAGT TCAGTGCCAT ATTTACATAT ACACTCCAGA 
CAGGGCAGTA TGATTCAGAC ACTTGCTATG AAGAAGGCTT CAAAGGGAAG GAGACGGTTG 
TCTCCTCCTA CTTTGTTGCC AAATTCTCCT TCTCACTTGA GTGAACTCAC TTCTCTGAAG 
GAAGCTACTC CTTCCCCAAT TAGTGAATCT CATAGTGATG AGACCATTCC TAGTGATAGT 
GGAATTGGAA CAGATAATAA TAGCACATCA GACAGGGCAG AAAAGTTTTG TGGGCAGAAA 
AAGAGGAGGC ATTCTTTTGA ACATGTTTCT CTCATTCCCC CTGAAACCTC CACAGTCCTA 
AGTAGTCTCA AAGAGAAACA TAAACACAAA TGTAAACGCA GGAGTCATGA TTACCTCAGT 
TATGACAAAA TGAAAAGACA GAAGCGAAAA CGGAAAAAGA AGTATCCCCA GTTACGAAAC 
AGACAGGATC CAGACTTTCT TGCTGAGCTG GAGGAATTAA TAAGTCGTTT AAGTGAAATT 
CGGATCACTC ATCGAAGTCA TCATTTTATT CCCCGTGATC TCCTACCCAC TATTTTTCGA 
ATTAACTTTA ACAGTTTCTA TACGCACCCC ACTTTTCCCT TAGACCCTTT GCACTACATT 
AGAAAACCTG ACTTAAAGAA AAAAAGAGGG AGGCCTCCCA AGATGAGGGA AGCAATGGCT 
GAAATGCCTT TTATGCATAG CCTTAGTTTC CCTCTCTCTA GTACTGGGTT TTACCCCTCT 
TATGGCATGC CTTATTCTCC CTCTCCACTT GCAGCTGCTC CCATAGGATT AGGATACTAT 
GGAAGGTACC CTCCAACTCT TTATCCACCA CCCCCCTCTC CTTCTTTCAC CACTCCTTTG 
CCACCTCCTT CTTACATGCA TGCTGGTCAT TTGCTTCTCA ATCCTGCCAA GTACCACAAG 
AAGAAACATA AGCTGCTTCG TCAGGAAGCC TTTCTTACAA CAAGTAGGAC ACCCCTTCTC 
TCCATGAGCA CCTACCCCAG TGTCCCTCCT GAGATGGCCT ATGGTTGGAT GGTGGAGCAT 
AAACACCGGC ACCGGCACAA ACACAGAGAA CACCGTTCTT CTGAGCAACC CCAGGTTTCC 
ATGGACACTT TAGCTACCAA TAGCTCTTCC AGAACTGTCC TAGAATCGTT GAAGCGCTAT 
CGATTTGGTA AAGATGCTGT TGGGGAGCGA TATAAACACA AAGAGAAGCA TCGTTGTCAC 
ATGTCCTGTC CTCATCTGTC CCCATCAAAA AGCTTATTAA GCCGAGAGGA GCAATGGGTC 
CACCGAGAGC CTTCAGAATC CACTTCATTG GCCTTGGGGT TGCAGACACC TTTACAGATT 
GACTGTTCAG AAACGTCTCC AGGTTTGTCC CTGGGAGGTT TTACACCTAG CTCTGAGCCA 
GCCAGCAGTG ATGAACACAC AAACCTTTTC ACAAGTGCAA TAGGCAGCTG CAGAGTATCA 
AACTCTACTA ATACAAGTGG CCGGAAGAAA TTGACTGACA GTCCAGGACT CTTTACCGCA 
CAGGACACTT CATTAAATCG GCCTCTTAGA AAGGAAACTT TGCCTTCCAA TGAAAGGGCA 
CTGCAAACTT TGACAGGCTC CCAGCCAGTC CTAGACAAGC CTTCCCAAAG GCCATCAGAG 
AGCACAAACT GTAGCCCTAC TCGAAAGAGA TCTTCATCAG AGAGCACTTC TTCAACAGTG 
ATTGGAATCC CTTCCCGAGG TACAAGATTA GGAACATCTG TGGATGACTC TGTGGATAGC 
CTGCTACAGC GTATGGCGCA ACATGAAAGC CAAGGGACCT TAGAGAAAAA CATTGATGCT 
GTCATTGCCA CAGTGTCCAT CCCACCTTCC TCAAGCCCAG GTCATACTCA TGGTAGGGAA 
CGAAGTCTGG GGAAATCAGA CAGTCTGCTA GTACCCACAG CACCTAGTGA CTCCTGCAGC 
AACACCACCA CCACCATCTC ACTCCTTTCT GAGAGGTTGC CAAGCAGCTG TTCCCCCCAT 
CATCTCAAAA GGAGTGTGGT AGAAGCCATG CAACGCCAAG CCCGGAAAAT GTGCAATTAT 
AACAAAATCT TGGCCACCAA GAAAAACCTA GACCATGTCA ATAAAATCTT AAAAGCCAAA 
AAGCTTCAAA GACAGGCAAG GACAGGTAAC AATTTTGTGA AGCGCAGACC AGGCCGACCC 
CGGAAATATC CCCTCCAGGC TGTGGTGTCA ATGCAGGCAT TCCAGGCTGC CCAATTTGCC 
AGCCCAGAAT TAGATGAAGG GGAGGAAGGC AGAGCACTAC ATCTTGGTCC TGATACAGTT 
ACAGATGTCA TTGAGGCTGT TGTTCAGAGT GTGAACCTGA ACCCAGAACT TAGAAAAGGA 
TGGAAAAGGA AAAGTTGGCT TCTGGAGGAA GAGGCAAGGA AAAGACAGAA GATACTTCCA 
GAGGAAGAAC AGGAAAATAA TAAAAGCTTT CCTGAGACAA CAATTGAATC TCCTAGTCCT 
CCAGAAGCCC CACCAAAGCC ACCTGAACCT GAAAATACCC TGCAGCCTTT GCCTTCTCTT 
ATCCCTCGGG AAAAGAAGGC CCCACGTCCC CCAAAGAAGA AATATCAGAA AGCAGGGCTC 
TATTCTGATG TATACAAAAC TACAGATCCA AAGAGCAGAC TGATACAATT AAAGAAAGAA 
AAACTGGAGT ATACTCCTGG AGAACATGAA TATGGATTAT TCCCAGCCCC TATTCATGTT 
GGAAAGTATT TGAGACAAAA GAGAATTGAC TTTCAGCTTC CTTATGACAT CCTCTGGCAA 
TGGAAACACA ATCAGCTATA TAAAAAGCCA GATGTCCCAC TATACAAAAA AATCCGTTCA 
AATGTCTACG TTGATGTCAA ACCTCTTTCT GGCTATGAGG CTACCACCTG CAACTGTAAA 
AAACCAGATG ATGGTGATAG GAAGGGTTGT ATGGATGACT GCCTCAACAG AATGATCTTT 
GCTGAGTGTT CCCCCAATAC CTGCCCCTGT GGAGAGCAGT GCTGCAATCA GAGGATACAA 
AGGCATGAGT GGGTACAGTG TCTTGAGCGG TTCCGAGCTG AGGAGAAGGG ATGGGGGATC 
CGGACCAAAG AACCTTTGAA AGCTGGCCAG TTCATTATTG AATACTTGGG AGAAGTTGTC 
AGTGAGCAGG AGTTCAGAAA CCGAATGATT GAACAGTACC ATAATCACAG TGACCATTAC 
TGCCTAAATC TGGACAGTGG AATGGTGATT GACAGCTACC GAATGGGCAA TGAGGCCCGG 
TTCATCAACC ACAGCTGTAA CCCAAATTGT GAAATGCAGA AATGGTCTGT TAATGGAGTC 
TACCGGATTG GGCTCTATGC CCTGAAAGAT ATGCCAGCTG GTACTGAACT AACCTATGAC 
TACAATTTTC ATTCTTTTAA TGTTGAGAAA CAGCAACTTT GTAAGTGTGG TTTTGACAAA 
TGCCGGGGTA TCATCGGAGG CAAGAGCCAA CGCATGAATG GACTCACCAG CAAAAGCAGC 
CAACCTGTGA CCACACACAG ACGGCCAGGA CGGTCAAAAG AGAAGAGAAA ATCCAAGCAC 
AAGTTGAAGA AAAGGAGAGG CCATCTTTCT GAGGAGCCTA ATGAAAATGT CAGTACCCCA 
ACCAGAATGA CCCCCCAACT GCAAATGAAG CCAATGTCCA ACCGTGAAAG GAACTTTGTG 
TTAAAACATC ATGTATTCCT GGTCCGGAAC TGGGAGAAAA TCAGACAGAA GCAAGAGGAG 
GTAAAACACA CCAGTGACAA TATCCATTCA GCTTCCTTAT ATACCCGCTG GAATGGTATC 
TGCCGAGATG ATGGAAACAT CAAATCTGAT GTCTTCATGA CCCAGTTCTC CGCCTTGCAG 
ACAGCCCGCT CAGTTCGTAC AAGGAGATTG GCAGCTGCAG AGGAGAACAT TGAAGTGGCC 
CGGGCAGCCC GCCTGGCCCA GATATTCAAA GAGATATGTG ACAGCATCAT CTCCTACAAA 
GATTCCTCCC GACAGGCATT GGCAACTCCA CTGTTGAACC TTCCCCCAAA GAAAAAGAAT 
GCTGATTATT ATGAGAAGAT ATCTGACCCC TTAGATCTCA TTACCATTGA GAAGCAGATC 
CTGACAGGGT ATTACAAGAC AGTGGAGGCG TTTGATGCTG ACATGCTCAA GGTCTTCCGG 
AATGCTGAGA AATACTATGG ACGCAAGTCC CCAATTGGGA GAGATGTATG TCGACTACGC 
AAAGCATACT ACAATGCACG GCATGAGGCA TCAGCTCAAA TTGATGAGAT TGTGGGGGAA 
ACAGCAAGTG AAGCAGACAG CAGCGAAACC TCAGTCTGTG AGAAGGAAAA TGGGCATGAG 
AAGGATGATG ATGTTATCCG CTGTATCTGC GGCCTCTACA AGGATGAAGG CCTCATGATT 
CAGTGTGACA AGTGCATGGT GTGGCAGCAC TGTGATTGCA TGGGAGTAAA CTCAGATGTA 
GAACATTACC TCTGTGAACA ATGTGATCCA AGGCCTGTGG ACAGGGAAGT GCCCATGATA 
CCTCGGCCCC ATTATGCCCA ACCTGGCTGT GTCTACTTCA TCTGTCTACT CCGGGATGAC 
CTGCTGCTAC GTCAAGGTGA CTGTGTGTAC CTGATGCGAG ATAGTCGGAG AACCCCTGAT 
GGCCACCCAG TCCGTCAGTC TTATCGATTG TTATCCCATA TCAACCGAGA GAAACTTGAT 
ATCTTTCGAA TTGAGAAGCT TTGGAAGAAT GAAAAAGAGG AGCGGTTTGC CTTTGGTCAT 
CATTATTTTC GTCCCCATGA AACCCACCAC TCCCCATCAC GTCGATTTTA TCACAATGAG 
CTGTTCCGAG TGCCACTGTA TGAAATCATT CCTCTGGAGG CTGTGGTGGG GACCTGTTGC 
GTATTAGATC TGTACACATA TTGTAAAGGA AGGCCCAAAG GAGTGAAAGA GCAGGATGTA 
TATATCTGTG ACTACCGACT TGATAAATCA GCCCACCTGT TCTACAAGAT TCATCGGAAC 
CGCTATCCTG TCTGCACCAA ACCCTATGCC TTTGACCACT TCCCCAAGAA GCTCACTCCC 
AAGAGAGATT TCTCACCTCA TTATGTCCCA GACAACTACA AGAGGAATGG AGGAAGATCA 
TCCTGGAAGA CAGAACGTTC AAAGCCACCC ATCAAAGACC TAGGCCAGGA GGATGATGCC 
CTACCACTCA TTGAAGAAGT ACTGGCCAGT CAAGAGCAAG CAGCCAATGA GATGCCCACC 
CTAGAAGAAA CTGATCGGGA GGTAGCACCT GGTGAAACAA CTGAGGGTGA AAAGAAAGCT 
GATGAAAGCA GACAGGAGCC TCAGAGGTCC TGCACACCTG AAGAGAGGAG ACACAGCCAA 
CGGGAGCGAC TCAACCAGAT TCTGCTCAAC CTTCTTGAGA AGATCCCTGG GAAGAATGCC 
ATCGATGTGA CATACTTGCT GGAGGAAGGA TCAGGAAGAA AGCTTCGAAG ACGTACTCTG 
CTTATCCCAG AGAGCAGCTT CCGAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASH1L ( XM_023502250.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023358018.1 Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X1, mRNA.

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CloneID	OSa154248
Clone ID Related Accession (Same CDS sequence)	XM_023502250.1 , XM_031966722.1 , XM_031966719.1 , XM_031966721.1 , XM_031966720.1
Accession Version	XM_023502250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	histone-lysine N-methyltransferase ASH1L isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838832.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATA GGAATACTGC TATGTTAGGA GTGGGTACCG ACTCCGAAGG ATTTTCAAGG 
AAGAGTCCTT CTGCTGTCAG CACTGGAACA CTGGTTAGTA AGAGAGAAGT GGAACTAGAG 
AATAGCACTA AGGAAGAAGA GGATCTTTGG AGGCGGAATA GGGACAGAAA CATTGAGACA 
GGGAAAAATG ATGGTTTGAC TGATGCACAG CAGTTTTCTG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAGTTGAA AATTGGCCTT CAGGCTAAGA GAACTAAAAA GCCTCCTAAA 
AACTTGGAGA ACTATGTATG TCGACCTGCT ATAAAAACAA GCATTAAGCA TCCAAGGAAA 
GCATTGAAAA GTGGGAAGAT GACAGATGAA AAAAATGAAC TTTGCCCTTC AAAACGAACT 
ATGTCGGGAA TATATATCTC CACATTGCAA AACAACATGT ATGTTACACA TTGGAGGAGG 
GGAGAAAAAG ACCCTTCAAA ACTTTACAAG AAAGCAAATG ATGTTGCAGC TGTTGAATGC 
CGCTCTGAAG AAGGCATCCA CCTTCATTCA CAGGGAGAAA ATAATCCTGT GTCAAAGAAG 
CTTTCTCCAC TACACTCTGA AATGACAGAT TATGTTAAAT CAGCACCATC TACTCTTGTT 
GGTAACCGTG ATCCTGACTC GAAGGACAGA GCTCTACTTA ATGGAGCAAC AAGTGTAACA 
GAAAAATTGG CACAGCTCAT TGCTACTTGT CCACCCTCCA AGTCTTCCAA GACAAAGCCA 
AAAAAGTTAG GGGCTGGTAT TACAGCAGGA TTGGTTAGCA AAGACTTGAT CAGAAAGCCA 
GGCATTGGTC CTATGGCAGG AATTATTCAC AAGGACTTGA TTAAGAAACC AACAATTAGC 
CCAGCAGTTG GGTTGGTAAC TAAAGACCCT GGGAAAAAGC CAATCTTTAG TGCTGCAGTA 
GGATTGGTCA GTAAAGACTC TGGGAAAAAA TTAGGAACTG GGACTACTGC GGTATTCATA 
AACAAGGACT TAGGAAAGAA ACCAGGGACT ATCACTACAG TGGGACTGGT GAGCAGAGAG 
TCAGGGAAGA AATTAGGAAT TGGTATCGTC CCAGGTTTGG TGAGTAAAGA ATCAGGAAAG 
AAGTTGGGAC TTGGTACTGT AGTTGGGCTT GTTAATAAAG ATTCAGTAAA GAAGTTTGGC 
CCTGCCATTG GACTGGTTGC AAAGGACTGT GGGAAGAAGA CTATAACAAA TTCATCAGTG 
GGATTGGTTA ATAAGGACCC TGCAAAGAAA TTGGTGGCTT GCCCTGTAGC AGGATTGGTC 
AACAAAGACA TTGTGAATTT AAAAGCTGAA GCCTTGCTCC CTGTTCAAGA ACCACTTAAG 
GTTTCTTGCA ATACAAATAC TAGTAGTCAT GAAAGTCAGG AGCATTCTGA ATCCCTGAAA 
GATAGTGCCA CCAGCAAAGC TTTTGAGAAG ACTATTATTC GTCAGAATAA AGATAGTTTA 
TTAGAAAAGT TCTCAATTCG AAAAGAAATC ATTACTTTGG ACAAAGACAT TTTTAATGAA 
GGAACTTGCA TACAGCAGGA CAGTTTCTTA TCCAGTGAAA GGGGGACCTA TGTTGAAACT 
ACAAAGCATG AGAAGCAGCC TCCAGTGTAT TGCACTTCAC CAGATTTTCA AATGGGAGGC 
ACTTCTGATG CATCAACAGC TAAGTCTCCC TTTAGTGCAA TAGGGGAGAG TAATCTCCCT 
TCCCCTTCAC CAGCCTTGTC TGTTACGCCC TTAAACAGAA GTCCCCCTGG AACTTCTTCT 
CAGTTGGCTA CTAATCCATT ACATTTAGGT CCTTCCACAG AACTCATAGA AGGAATTGCT 
GAATCTATTG GAAAAACCCA ATTCAGTTCT GAAAATTCCC ATATGAACCT TGGTCACAGG 
TCTCTGGTTC ACAGCATGAA CACTGATTGC AAAGGAAATG ATAAAGAGTT AAATGATTCA 
AAAGCTTCTC ACACAGATAA CTCAAGAATG GGCTCCTCCA TAGGAAAAAA GCCAAGTCTG 
GCTTCAGAAT CCAGTATTCA TGCTACAATT ACTCCCTCAG TTGTTAGTTT CACTAGTTTA 
TTTAGCAGTA AACCCTTTCT GAAGATTGGT GCAGTAACTG CATCAGAGAA ACACTGCCAA 
GTTGCTGAAA GCCTGAGCAC TAGTTTGCAG TCCAAACCAT TAAAAAAAAG AAAAGGAAGG 
AAACCACGAT GGACTAAAGT GGTAGCAAGA AACACATGCC GGCCTTCAAA AGGGCTAGAA 
TTAGAAAGGT CAGAGCTTTT TAAAAATGTT TCATGTAGCT CTTTGTCAAA TAGCAATTCC 
GAGCCAGCCA AGTTTATGAA AAACATTGGG CCATCTTCAT TTGTGGAGCA TGACTTCCTT 
AAATGCCGAT TGCCAAAGCT GAGTAAATCT AGCACAGCTC CATCTCTTGC TCTCTTAACT 
GAAACTGACA AGCAGTCTCA TAAGTCCTTT GCCAGCCACA AACTTTCCTC TAGTATATGC 
ATGTCTGGTG ACCTTTTACC TGACATTTAT AAACCTAAAA GAGGAAGACC TAAAACTAAG 
GAGATGCCCC AACTAGAAGG TCCACCCAAA AGGACCCTAA AAATCCCGGC CTCTAAAGTT 
TTTTCATTAC AGTCAAAAGA GGAGCAAGAA CCCCCTATTT TGCAGCCAGA AATTGAAATC 
CCTTCCTTCA AGCAGAGCCT ATCAGTATCA CCCTTTCCCA AAAAAAGAGG AAGGCCAAAA 
AGACAAATCA GATCACCAGT CAAGATGAAA CCACCTGTGC TATCAGTGGC ACCCTTTGTA 
GCCACTGAAA GTCCAAGTAA ACTAGAGTCT GAGAGTGATA AACATAGAAG TAGTAGTGAC 
TTCTTTGAAA GGAAAGATCA ACTTCAGGGT CCAGATGACA GTCATAAACC AAGTGTGTGT 
GGTATGAGTG ACCTTGAGAT GGAACCTGAT AAAAAAATTA ATAAGAGAAA CAATGGCCAA 
TTAATGAAAA CTATCATTCG CAAAATAAAT AAGATGAAGA CTTTGAAGAG AAAGAAACTG 
CTGAATCAGA TTCTCTCAAG TTCTGTTGAA TCAAGTAACA AAGGGAAAGT GCAATCCAAA 
CTCCACAATA CAGTGTCAAC ACTTGCTGCC ACTTTTGGCT CCAAACTGGG CCAACAGATA 
AATGTCAGCA AGAAAGGAAC CATTTATATA GGAAAGAGGC GAGGCCGGAA ACCAAAAGCT 
GTGCTGAACG GCATCTTAAC TAGTAGCCCA GCTAGTCTGG CTGTCCTAGA GAAAACAGCT 
CATCAGGTAG CTGGGTCAGC ATTAGGACAG ATTCTCCCCC CACTCTTGCC TTCAGCAGCA 
AGTAGTTCTG AGGTTCTTCC ATCCCCCATC TGTTCTCAGT CTTCTGGGAC TAGTGGGGGC 
CAGAGTCCTG TCAGCAGTGA TGCAGGATTT GTTGAGCCCA GTTCAGTGCC ATATTTACAT 
ATACACTCCA GACAGGGCAG TATGATTCAG ACACTTGCTA TGAAGAAGGC TTCAAAGGGA 
AGGAGACGGT TGTCTCCTCC TACTTTGTTG CCAAATTCTC CTTCTCACTT GAGTGAACTC 
ACTTCTCTGA AGGAAGCTAC TCCTTCCCCA ATTAGTGAAT CTCATAGTGA TGAGACCATT 
CCTAGTGATA GTGGAATTGG AACAGATAAT AATAGCACAT CAGACAGGGC AGAAAAGTTT 
TGTGGGCAGA AAAAGAGGAG GCATTCTTTT GAACATGTTT CTCTCATTCC CCCTGAAACC 
TCCACAGTCC TAAGTAGTCT CAAAGAGAAA CATAAACACA AATGTAAACG CAGGAGTCAT 
GATTACCTCA GTTATGACAA AATGAAAAGA CAGAAGCGAA AACGGAAAAA GAAGTATCCC 
CAGTTACGAA ACAGACAGGA TCCAGACTTT CTTGCTGAGC TGGAGGAATT AATAAGTCGT 
TTAAGTGAAA TTCGGATCAC TCATCGAAGT CATCATTTTA TTCCCCGTGA TCTCCTACCC 
ACTATTTTTC GAATTAACTT TAACAGTTTC TATACGCACC CCACTTTTCC CTTAGACCCT 
TTGCACTACA TTAGAAAACC TGACTTAAAG AAAAAAAGAG GGAGGCCTCC CAAGATGAGG 
GAAGCAATGG CTGAAATGCC TTTTATGCAT AGCCTTAGTT TCCCTCTCTC TAGTACTGGG 
TTTTACCCCT CTTATGGCAT GCCTTATTCT CCCTCTCCAC TTGCAGCTGC TCCCATAGGA 
TTAGGATACT ATGGAAGGTA CCCTCCAACT CTTTATCCAC CACCCCCCTC TCCTTCTTTC 
ACCACTCCTT TGCCACCTCC TTCTTACATG CATGCTGGTC ATTTGCTTCT CAATCCTGCC 
AAGTACCACA AGAAGAAACA TAAGCTGCTT CGTCAGGAAG CCTTTCTTAC AACAAGTAGG 
ACACCCCTTC TCTCCATGAG CACCTACCCC AGTGTCCCTC CTGAGATGGC CTATGGTTGG 
ATGGTGGAGC ATAAACACCG GCACCGGCAC AAACACAGAG AACACCGTTC TTCTGAGCAA 
CCCCAGGTTT CCATGGACAC TTTAGCTACC AATAGCTCTT CCAGAACTGT CCTAGAATCG 
TTGAAGCGCT ATCGATTTGG TAAAGATGCT GTTGGGGAGC GATATAAACA CAAAGAGAAG 
CATCGTTGTC ACATGTCCTG TCCTCATCTG TCCCCATCAA AAAGCTTATT AAGCCGAGAG 
GAGCAATGGG TCCACCGAGA GCCTTCAGAA TCCACTTCAT TGGCCTTGGG GTTGCAGACA 
CCTTTACAGA TTGACTGTTC AGAAACGTCT CCAGGTTTGT CCCTGGGAGG TTTTACACCT 
AGCTCTGAGC CAGCCAGCAG TGATGAACAC ACAAACCTTT TCACAAGTGC AATAGGCAGC 
TGCAGAGTAT CAAACTCTAC TAATACAAGT GGCCGGAAGA AATTGACTGA CAGTCCAGGA 
CTCTTTACCG CACAGGACAC TTCATTAAAT CGGCCTCTTA GAAAGGAAAC TTTGCCTTCC 
AATGAAAGGG CACTGCAAAC TTTGACAGGC TCCCAGCCAG TCCTAGACAA GCCTTCCCAA 
AGGCCATCAG AGAGCACAAA CTGTAGCCCT ACTCGAAAGA GATCTTCATC AGAGAGCACT 
TCTTCAACAG TGATTGGAAT CCCTTCCCGA GGTACAAGAT TAGGAACATC TGTGGATGAC 
TCTGTGGATA GCCTGCTACA GCGTATGGCG CAACATGAAA GCCAAGGGAC CTTAGAGAAA 
AACATTGATG CTGTCATTGC CACAGTGTCC ATCCCACCTT CCTCAAGCCC AGGTCATACT 
CATGGTAGGG AACGAAGTCT GGGGAAATCA GACAGTCTGC TAGTACCCAC AGCACCTAGT 
GACTCCTGCA GCAACACCAC CACCACCATC TCACTCCTTT CTGAGAGGTT GCCAAGCAGC 
TGTTCCCCCC ATCATCTCAA AAGGAGTGTG GTAGAAGCCA TGCAACGCCA AGCCCGGAAA 
ATGTGCAATT ATAACAAAAT CTTGGCCACC AAGAAAAACC TAGACCATGT CAATAAAATC 
TTAAAAGCCA AAAAGCTTCA AAGACAGGCA AGGACAGGTA ACAATTTTGT GAAGCGCAGA 
CCAGGCCGAC CCCGGAAATA TCCCCTCCAG GCTGTGGTGT CAATGCAGGC ATTCCAGGCT 
GCCCAATTTG CCAGCCCAGA ATTAGATGAA GGGGAGGAAG GCAGAGCACT ACATCTTGGT 
CCTGATACAG TTACAGATGT CATTGAGGCT GTTGTTCAGA GTGTGAACCT GAACCCAGAA 
CTTAGAAAAG GATGGAAAAG GAAAAGTTGG CTTCTGGAGG AAGAGGCAAG GAAAAGACAG 
AAGATACTTC CAGAGGAAGA ACAGGAAAAT AATAAAAGCT TTCCTGAGAC AACAATTGAA 
TCTCCTAGTC CTCCAGAAGC CCCACCAAAG CCACCTGAAC CTGAAAATAC CCTGCAGCCT 
TTGCCTTCTC TTATCCCTCG GGAAAAGAAG GCCCCACGTC CCCCAAAGAA GAAATATCAG 
AAAGCAGGGC TCTATTCTGA TGTATACAAA ACTACAGATC CAAAGAGCAG ACTGATACAA 
TTAAAGAAAG AAAAACTGGA GTATACTCCT GGAGAACATG AATATGGATT ATTCCCAGCC 
CCTATTCATG TTGGAAAGTA TTTGAGACAA AAGAGAATTG ACTTTCAGCT TCCTTATGAC 
ATCCTCTGGC AATGGAAACA CAATCAGCTA TATAAAAAGC CAGATGTCCC ACTATACAAA 
AAAATCCGTT CAAATGTCTA CGTTGATGTC AAACCTCTTT CTGGCTATGA GGCTACCACC 
TGCAACTGTA AAAAACCAGA TGATGGTGAT AGGAAGGGTT GTATGGATGA CTGCCTCAAC 
AGAATGATCT TTGCTGAGTG TTCCCCCAAT ACCTGCCCCT GTGGAGAGCA GTGCTGCAAT 
CAGAGGATAC AAAGGCATGA GTGGGTACAG TGTCTTGAGC GGTTCCGAGC TGAGGAGAAG 
GGATGGGGGA TCCGGACCAA AGAACCTTTG AAAGCTGGCC AGTTCATTAT TGAATACTTG 
GGAGAAGTTG TCAGTGAGCA GGAGTTCAGA AACCGAATGA TTGAACAGTA CCATAATCAC 
AGTGACCATT ACTGCCTAAA TCTGGACAGT GGAATGGTGA TTGACAGCTA CCGAATGGGC 
AATGAGGCCC GGTTCATCAA CCACAGCTGT AACCCAAATT GTGAAATGCA GAAATGGTCT 
GTTAATGGAG TCTACCGGAT TGGGCTCTAT GCCCTGAAAG ATATGCCAGC TGGTACTGAA 
CTAACCTATG ACTACAATTT TCATTCTTTT AATGTTGAGA AACAGCAACT TTGTAAGTGT 
GGTTTTGACA AATGCCGGGG TATCATCGGA GGCAAGAGCC AACGCATGAA TGGACTCACC 
AGCAAAAGCA GCCAACCTGT GACCACACAC AGACGGCCAG GACGGTCAAA AGAGAAGAGA 
AAATCCAAGC ACAAGTTGAA GAAAAGGAGA GGCCATCTTT CTGAGGAGCC TAATGAAAAT 
GTCAGTACCC CAACCAGAAT GACCCCCCAA CTGCAAATGA AGCCAATGTC CAACCGTGAA 
AGGAACTTTG TGTTAAAACA TCATGTATTC CTGGTCCGGA ACTGGGAGAA AATCAGACAG 
AAGCAAGAGG AGGTAAAACA CACCAGTGAC AATATCCATT CAGCTTCCTT ATATACCCGC 
TGGAATGGTA TCTGCCGAGA TGATGGAAAC ATCAAATCTG ATGTCTTCAT GACCCAGTTC 
TCCGCCTTGC AGACAGCCCG CTCAGTTCGT ACAAGGAGAT TGGCAGCTGC AGAGGAGAAC 
ATTGAAGTGG CCCGGGCAGC CCGCCTGGCC CAGATATTCA AAGAGATATG TGACAGCATC 
ATCTCCTACA AAGATTCCTC CCGACAGGCA TTGGCAACTC CACTGTTGAA CCTTCCCCCA 
AAGAAAAAGA ATGCTGATTA TTATGAGAAG ATATCTGACC CCTTAGATCT CATTACCATT 
GAGAAGCAGA TCCTGACAGG GTATTACAAG ACAGTGGAGG CGTTTGATGC TGACATGCTC 
AAGGTCTTCC GGAATGCTGA GAAATACTAT GGACGCAAGT CCCCAATTGG GAGAGATGTA 
TGTCGACTAC GCAAAGCATA CTACAATGCA CGGCATGAGG CATCAGCTCA AATTGATGAG 
ATTGTGGGGG AAACAGCAAG TGAAGCAGAC AGCAGCGAAA CCTCAGTCTG TGAGAAGGAA 
AATGGGCATG AGAAGGATGA TGATGTTATC CGCTGTATCT GCGGCCTCTA CAAGGATGAA 
GGCCTCATGA TTCAGTGTGA CAAGTGCATG GTGTGGCAGC ACTGTGATTG CATGGGAGTA 
AACTCAGATG TAGAACATTA CCTCTGTGAA CAATGTGATC CAAGGCCTGT GGACAGGGAA 
GTGCCCATGA TACCTCGGCC CCATTATGCC CAACCTGGCT GTGTCTACTT CATCTGTCTA 
CTCCGGGATG ACCTGCTGCT ACGTCAAGGT GACTGTGTGT ACCTGATGCG AGATAGTCGG 
AGAACCCCTG ATGGCCACCC AGTCCGTCAG TCTTATCGAT TGTTATCCCA TATCAACCGA 
GAGAAACTTG ATATCTTTCG AATTGAGAAG CTTTGGAAGA ATGAAAAAGA GGAGCGGTTT 
GCCTTTGGTC ATCATTATTT TCGTCCCCAT GAAACCCACC ACTCCCCATC ACGTCGATTT 
TATCACAATG AGCTGTTCCG AGTGCCACTG TATGAAATCA TTCCTCTGGA GGCTGTGGTG 
GGGACCTGTT GCGTATTAGA TCTGTACACA TATTGTAAAG GAAGGCCCAA AGGAGTGAAA 
GAGCAGGATG TATATATCTG TGACTACCGA CTTGATAAAT CAGCCCACCT GTTCTACAAG 
ATTCATCGGA ACCGCTATCC TGTCTGCACC AAACCCTATG CCTTTGACCA CTTCCCCAAG 
AAGCTCACTC CCAAGAGAGA TTTCTCACCT CATTATGTCC CAGACAACTA CAAGAGGAAT 
GGAGGAAGAT CATCCTGGAA GACAGAACGT TCAAAGCCAC CCATCAAAGA CCTAGGCCAG 
GAGGATGATG CCCTACCACT CATTGAAGAA GTACTGGCCA GTCAAGAGCA AGCAGCCAAT 
GAGATGCCCA CCCTAGAAGA AACTGATCGG GAGGTAGCAC CTGGTGAAAC AACTGAGGGT 
GAAAAGAAAG CTGATGAAAG CAGACAGGAG CCTCAGAGGT CCTGCACACC TGAAGAGAGG 
AGACACAGCC AACGGGAGCG ACTCAACCAG ATTCTGCTCA ACCTTCTTGA GAAGATCCCT 
GGGAAGAATG CCATCGATGT GACATACTTG CTGGAGGAAG GATCAGGAAG AAAGCTTCGA 
AGACGTACTC TGCTTATCCC AGAGAGCAGC TTCCGAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023358018.1
CDS	93..9074
Translation	MDHRNTAMLGVGTDSEGFSRKSPSAVSTGTLVSKREVELENSTKEEEDLWRRNRDRNIETGKNDGLTDAQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTSIKHPRKALKSGKMTDEKNELCPSKRTMSGIYISTLQNNMYVTHWRRGEKDPSKLYKKANDVAAVECRSEEGIHLHSQGENNPVSKKLSPLHSEMTDYVKSAPSTLVGNRDPDSKDRALLNGATSVTEKLAQLIATCPPSKSSKTKPKKLGAGITAGLVSKDLIRKPGIGPMAGIIHKDLIKKPTISPAVGLVTKDPGKKPIFSAAVGLVSKDSGKKLGTGTTAVFINKDLGKKPGTITTVGLVSRESGKKLGIGIVPGLVSKESGKKLGLGTVVGLVNKDSVKKFGPAIGLVAKDCGKKTITNSSVGLVNKDPAKKLVACPVAGLVNKDIVNLKAEALLPVQEPLKVSCNTNTSSHESQEHSESLKDSATSKAFEKTIIRQNKDSLLEKFSIRKEIITLDKDIFNEGTCIQQDSFLSSERGTYVETTKHEKQPPVYCTSPDFQMGGTSDASTAKSPFSAIGESNLPSPSPALSVTPLNRSPPGTSSQLATNPLHLGPSTELIEGIAESIGKTQFSSENSHMNLGHRSLVHSMNTDCKGNDKELNDSKASHTDNSRMGSSIGKKPSLASESSIHATITPSVVSFTSLFSSKPFLKIGAVTASEKHCQVAESLSTSLQSKPLKKRKGRKPRWTKVVARNTCRPSKGLELERSELFKNVSCSSLSNSNSEPAKFMKNIGPSSFVEHDFLKCRLPKLSKSSTAPSLALLTETDKQSHKSFASHKLSSSICMSGDLLPDIYKPKRGRPKTKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQIRSPVKMKPPVLSVAPFVATESPSKLESESDKHRSSSDFFERKDQLQGPDDSHKPSVCGMSDLEMEPDKKINKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSTLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKAVLNGILTSSPASLAVLEKTAHQVAGSALGQILPPLLPSAASSSEVLPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHIHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFLAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPTFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLAAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPAKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDTLATNSSSRTVLESLKRYRFGKDAVGERYKHKEKHRCHMSCPHLSPSKSLLSREEQWVHREPSESTSLALGLQTPLQIDCSETSPGLSLGGFTPSSEPASSDEHTNLFTSAIGSCRVSNSTNTSGRKKLTDSPGLFTAQDTSLNRPLRKETLPSNERALQTLTGSQPVLDKPSQRPSESTNCSPTRKRSSSESTSSTVIGIPSRGTRLGTSVDDSVDSLLQRMAQHESQGTLEKNIDAVIATVSIPPSSSPGHTHGRERSLGKSDSLLVPTAPSDSCSNTTTTISLLSERLPSSCSPHHLKRSVVEAMQRQARKMCNYNKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKYPLQAVVSMQAFQAAQFASPELDEGEEGRALHLGPDTVTDVIEAVVQSVNLNPELRKGWKRKSWLLEEEARKRQKILPEEEQENNKSFPETTIESPSPPEAPPKPPEPENTLQPLPSLIPREKKAPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPDDGDRKGCMDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCNPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFDKCRGIIGGKSQRMNGLTSKSSQPVTTHRRPGRSKEKRKSKHKLKKRRGHLSEEPNENVSTPTRMTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIRQKQEEVKHTSDNIHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENIEVARAARLAQIFKEICDSIISYKDSSRQALATPLLNLPPKKKNADYYEKISDPLDLITIEKQILTGYYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKAYYNARHEASAQIDEIVGETASEADSSETSVCEKENGHEKDDDVIRCICGLYKDEGLMIQCDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINREKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKTERSKPPIKDLGQEDDALPLIEEVLASQEQAANEMPTLEETDREVAPGETTEGEKKADESRQEPQRSCTPEERRHSQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLLIPESSFRK

Target ORF information:

RefSeq Version	XM_023502250.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023502250.1

ORF Insert Sequence:

ATGGACCATA GGAATACTGC TATGTTAGGA GTGGGTACCG ACTCCGAAGG ATTTTCAAGG 
AAGAGTCCTT CTGCTGTCAG CACTGGAACA CTGGTTAGTA AGAGAGAAGT GGAACTAGAG 
AATAGCACTA AGGAAGAAGA GGATCTTTGG AGGCGGAATA GGGACAGAAA CATTGAGACA 
GGGAAAAATG ATGGTTTGAC TGATGCACAG CAGTTTTCTG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAGTTGAA AATTGGCCTT CAGGCTAAGA GAACTAAAAA GCCTCCTAAA 
AACTTGGAGA ACTATGTATG TCGACCTGCT ATAAAAACAA GCATTAAGCA TCCAAGGAAA 
GCATTGAAAA GTGGGAAGAT GACAGATGAA AAAAATGAAC TTTGCCCTTC AAAACGAACT 
ATGTCGGGAA TATATATCTC CACATTGCAA AACAACATGT ATGTTACACA TTGGAGGAGG 
GGAGAAAAAG ACCCTTCAAA ACTTTACAAG AAAGCAAATG ATGTTGCAGC TGTTGAATGC 
CGCTCTGAAG AAGGCATCCA CCTTCATTCA CAGGGAGAAA ATAATCCTGT GTCAAAGAAG 
CTTTCTCCAC TACACTCTGA AATGACAGAT TATGTTAAAT CAGCACCATC TACTCTTGTT 
GGTAACCGTG ATCCTGACTC GAAGGACAGA GCTCTACTTA ATGGAGCAAC AAGTGTAACA 
GAAAAATTGG CACAGCTCAT TGCTACTTGT CCACCCTCCA AGTCTTCCAA GACAAAGCCA 
AAAAAGTTAG GGGCTGGTAT TACAGCAGGA TTGGTTAGCA AAGACTTGAT CAGAAAGCCA 
GGCATTGGTC CTATGGCAGG AATTATTCAC AAGGACTTGA TTAAGAAACC AACAATTAGC 
CCAGCAGTTG GGTTGGTAAC TAAAGACCCT GGGAAAAAGC CAATCTTTAG TGCTGCAGTA 
GGATTGGTCA GTAAAGACTC TGGGAAAAAA TTAGGAACTG GGACTACTGC GGTATTCATA 
AACAAGGACT TAGGAAAGAA ACCAGGGACT ATCACTACAG TGGGACTGGT GAGCAGAGAG 
TCAGGGAAGA AATTAGGAAT TGGTATCGTC CCAGGTTTGG TGAGTAAAGA ATCAGGAAAG 
AAGTTGGGAC TTGGTACTGT AGTTGGGCTT GTTAATAAAG ATTCAGTAAA GAAGTTTGGC 
CCTGCCATTG GACTGGTTGC AAAGGACTGT GGGAAGAAGA CTATAACAAA TTCATCAGTG 
GGATTGGTTA ATAAGGACCC TGCAAAGAAA TTGGTGGCTT GCCCTGTAGC AGGATTGGTC 
AACAAAGACA TTGTGAATTT AAAAGCTGAA GCCTTGCTCC CTGTTCAAGA ACCACTTAAG 
GTTTCTTGCA ATACAAATAC TAGTAGTCAT GAAAGTCAGG AGCATTCTGA ATCCCTGAAA 
GATAGTGCCA CCAGCAAAGC TTTTGAGAAG ACTATTATTC GTCAGAATAA AGATAGTTTA 
TTAGAAAAGT TCTCAATTCG AAAAGAAATC ATTACTTTGG ACAAAGACAT TTTTAATGAA 
GGAACTTGCA TACAGCAGGA CAGTTTCTTA TCCAGTGAAA GGGGGACCTA TGTTGAAACT 
ACAAAGCATG AGAAGCAGCC TCCAGTGTAT TGCACTTCAC CAGATTTTCA AATGGGAGGC 
ACTTCTGATG CATCAACAGC TAAGTCTCCC TTTAGTGCAA TAGGGGAGAG TAATCTCCCT 
TCCCCTTCAC CAGCCTTGTC TGTTACGCCC TTAAACAGAA GTCCCCCTGG AACTTCTTCT 
CAGTTGGCTA CTAATCCATT ACATTTAGGT CCTTCCACAG AACTCATAGA AGGAATTGCT 
GAATCTATTG GAAAAACCCA ATTCAGTTCT GAAAATTCCC ATATGAACCT TGGTCACAGG 
TCTCTGGTTC ACAGCATGAA CACTGATTGC AAAGGAAATG ATAAAGAGTT AAATGATTCA 
AAAGCTTCTC ACACAGATAA CTCAAGAATG GGCTCCTCCA TAGGAAAAAA GCCAAGTCTG 
GCTTCAGAAT CCAGTATTCA TGCTACAATT ACTCCCTCAG TTGTTAGTTT CACTAGTTTA 
TTTAGCAGTA AACCCTTTCT GAAGATTGGT GCAGTAACTG CATCAGAGAA ACACTGCCAA 
GTTGCTGAAA GCCTGAGCAC TAGTTTGCAG TCCAAACCAT TAAAAAAAAG AAAAGGAAGG 
AAACCACGAT GGACTAAAGT GGTAGCAAGA AACACATGCC GGCCTTCAAA AGGGCTAGAA 
TTAGAAAGGT CAGAGCTTTT TAAAAATGTT TCATGTAGCT CTTTGTCAAA TAGCAATTCC 
GAGCCAGCCA AGTTTATGAA AAACATTGGG CCATCTTCAT TTGTGGAGCA TGACTTCCTT 
AAATGCCGAT TGCCAAAGCT GAGTAAATCT AGCACAGCTC CATCTCTTGC TCTCTTAACT 
GAAACTGACA AGCAGTCTCA TAAGTCCTTT GCCAGCCACA AACTTTCCTC TAGTATATGC 
ATGTCTGGTG ACCTTTTACC TGACATTTAT AAACCTAAAA GAGGAAGACC TAAAACTAAG 
GAGATGCCCC AACTAGAAGG TCCACCCAAA AGGACCCTAA AAATCCCGGC CTCTAAAGTT 
TTTTCATTAC AGTCAAAAGA GGAGCAAGAA CCCCCTATTT TGCAGCCAGA AATTGAAATC 
CCTTCCTTCA AGCAGAGCCT ATCAGTATCA CCCTTTCCCA AAAAAAGAGG AAGGCCAAAA 
AGACAAATCA GATCACCAGT CAAGATGAAA CCACCTGTGC TATCAGTGGC ACCCTTTGTA 
GCCACTGAAA GTCCAAGTAA ACTAGAGTCT GAGAGTGATA AACATAGAAG TAGTAGTGAC 
TTCTTTGAAA GGAAAGATCA ACTTCAGGGT CCAGATGACA GTCATAAACC AAGTGTGTGT 
GGTATGAGTG ACCTTGAGAT GGAACCTGAT AAAAAAATTA ATAAGAGAAA CAATGGCCAA 
TTAATGAAAA CTATCATTCG CAAAATAAAT AAGATGAAGA CTTTGAAGAG AAAGAAACTG 
CTGAATCAGA TTCTCTCAAG TTCTGTTGAA TCAAGTAACA AAGGGAAAGT GCAATCCAAA 
CTCCACAATA CAGTGTCAAC ACTTGCTGCC ACTTTTGGCT CCAAACTGGG CCAACAGATA 
AATGTCAGCA AGAAAGGAAC CATTTATATA GGAAAGAGGC GAGGCCGGAA ACCAAAAGCT 
GTGCTGAACG GCATCTTAAC TAGTAGCCCA GCTAGTCTGG CTGTCCTAGA GAAAACAGCT 
CATCAGGTAG CTGGGTCAGC ATTAGGACAG ATTCTCCCCC CACTCTTGCC TTCAGCAGCA 
AGTAGTTCTG AGGTTCTTCC ATCCCCCATC TGTTCTCAGT CTTCTGGGAC TAGTGGGGGC 
CAGAGTCCTG TCAGCAGTGA TGCAGGATTT GTTGAGCCCA GTTCAGTGCC ATATTTACAT 
ATACACTCCA GACAGGGCAG TATGATTCAG ACACTTGCTA TGAAGAAGGC TTCAAAGGGA 
AGGAGACGGT TGTCTCCTCC TACTTTGTTG CCAAATTCTC CTTCTCACTT GAGTGAACTC 
ACTTCTCTGA AGGAAGCTAC TCCTTCCCCA ATTAGTGAAT CTCATAGTGA TGAGACCATT 
CCTAGTGATA GTGGAATTGG AACAGATAAT AATAGCACAT CAGACAGGGC AGAAAAGTTT 
TGTGGGCAGA AAAAGAGGAG GCATTCTTTT GAACATGTTT CTCTCATTCC CCCTGAAACC 
TCCACAGTCC TAAGTAGTCT CAAAGAGAAA CATAAACACA AATGTAAACG CAGGAGTCAT 
GATTACCTCA GTTATGACAA AATGAAAAGA CAGAAGCGAA AACGGAAAAA GAAGTATCCC 
CAGTTACGAA ACAGACAGGA TCCAGACTTT CTTGCTGAGC TGGAGGAATT AATAAGTCGT 
TTAAGTGAAA TTCGGATCAC TCATCGAAGT CATCATTTTA TTCCCCGTGA TCTCCTACCC 
ACTATTTTTC GAATTAACTT TAACAGTTTC TATACGCACC CCACTTTTCC CTTAGACCCT 
TTGCACTACA TTAGAAAACC TGACTTAAAG AAAAAAAGAG GGAGGCCTCC CAAGATGAGG 
GAAGCAATGG CTGAAATGCC TTTTATGCAT AGCCTTAGTT TCCCTCTCTC TAGTACTGGG 
TTTTACCCCT CTTATGGCAT GCCTTATTCT CCCTCTCCAC TTGCAGCTGC TCCCATAGGA 
TTAGGATACT ATGGAAGGTA CCCTCCAACT CTTTATCCAC CACCCCCCTC TCCTTCTTTC 
ACCACTCCTT TGCCACCTCC TTCTTACATG CATGCTGGTC ATTTGCTTCT CAATCCTGCC 
AAGTACCACA AGAAGAAACA TAAGCTGCTT CGTCAGGAAG CCTTTCTTAC AACAAGTAGG 
ACACCCCTTC TCTCCATGAG CACCTACCCC AGTGTCCCTC CTGAGATGGC CTATGGTTGG 
ATGGTGGAGC ATAAACACCG GCACCGGCAC AAACACAGAG AACACCGTTC TTCTGAGCAA 
CCCCAGGTTT CCATGGACAC TTTAGCTACC AATAGCTCTT CCAGAACTGT CCTAGAATCG 
TTGAAGCGCT ATCGATTTGG TAAAGATGCT GTTGGGGAGC GATATAAACA CAAAGAGAAG 
CATCGTTGTC ACATGTCCTG TCCTCATCTG TCCCCATCAA AAAGCTTATT AAGCCGAGAG 
GAGCAATGGG TCCACCGAGA GCCTTCAGAA TCCACTTCAT TGGCCTTGGG GTTGCAGACA 
CCTTTACAGA TTGACTGTTC AGAAACGTCT CCAGGTTTGT CCCTGGGAGG TTTTACACCT 
AGCTCTGAGC CAGCCAGCAG TGATGAACAC ACAAACCTTT TCACAAGTGC AATAGGCAGC 
TGCAGAGTAT CAAACTCTAC TAATACAAGT GGCCGGAAGA AATTGACTGA CAGTCCAGGA 
CTCTTTACCG CACAGGACAC TTCATTAAAT CGGCCTCTTA GAAAGGAAAC TTTGCCTTCC 
AATGAAAGGG CACTGCAAAC TTTGACAGGC TCCCAGCCAG TCCTAGACAA GCCTTCCCAA 
AGGCCATCAG AGAGCACAAA CTGTAGCCCT ACTCGAAAGA GATCTTCATC AGAGAGCACT 
TCTTCAACAG TGATTGGAAT CCCTTCCCGA GGTACAAGAT TAGGAACATC TGTGGATGAC 
TCTGTGGATA GCCTGCTACA GCGTATGGCG CAACATGAAA GCCAAGGGAC CTTAGAGAAA 
AACATTGATG CTGTCATTGC CACAGTGTCC ATCCCACCTT CCTCAAGCCC AGGTCATACT 
CATGGTAGGG AACGAAGTCT GGGGAAATCA GACAGTCTGC TAGTACCCAC AGCACCTAGT 
GACTCCTGCA GCAACACCAC CACCACCATC TCACTCCTTT CTGAGAGGTT GCCAAGCAGC 
TGTTCCCCCC ATCATCTCAA AAGGAGTGTG GTAGAAGCCA TGCAACGCCA AGCCCGGAAA 
ATGTGCAATT ATAACAAAAT CTTGGCCACC AAGAAAAACC TAGACCATGT CAATAAAATC 
TTAAAAGCCA AAAAGCTTCA AAGACAGGCA AGGACAGGTA ACAATTTTGT GAAGCGCAGA 
CCAGGCCGAC CCCGGAAATA TCCCCTCCAG GCTGTGGTGT CAATGCAGGC ATTCCAGGCT 
GCCCAATTTG CCAGCCCAGA ATTAGATGAA GGGGAGGAAG GCAGAGCACT ACATCTTGGT 
CCTGATACAG TTACAGATGT CATTGAGGCT GTTGTTCAGA GTGTGAACCT GAACCCAGAA 
CTTAGAAAAG GATGGAAAAG GAAAAGTTGG CTTCTGGAGG AAGAGGCAAG GAAAAGACAG 
AAGATACTTC CAGAGGAAGA ACAGGAAAAT AATAAAAGCT TTCCTGAGAC AACAATTGAA 
TCTCCTAGTC CTCCAGAAGC CCCACCAAAG CCACCTGAAC CTGAAAATAC CCTGCAGCCT 
TTGCCTTCTC TTATCCCTCG GGAAAAGAAG GCCCCACGTC CCCCAAAGAA GAAATATCAG 
AAAGCAGGGC TCTATTCTGA TGTATACAAA ACTACAGATC CAAAGAGCAG ACTGATACAA 
TTAAAGAAAG AAAAACTGGA GTATACTCCT GGAGAACATG AATATGGATT ATTCCCAGCC 
CCTATTCATG TTGGAAAGTA TTTGAGACAA AAGAGAATTG ACTTTCAGCT TCCTTATGAC 
ATCCTCTGGC AATGGAAACA CAATCAGCTA TATAAAAAGC CAGATGTCCC ACTATACAAA 
AAAATCCGTT CAAATGTCTA CGTTGATGTC AAACCTCTTT CTGGCTATGA GGCTACCACC 
TGCAACTGTA AAAAACCAGA TGATGGTGAT AGGAAGGGTT GTATGGATGA CTGCCTCAAC 
AGAATGATCT TTGCTGAGTG TTCCCCCAAT ACCTGCCCCT GTGGAGAGCA GTGCTGCAAT 
CAGAGGATAC AAAGGCATGA GTGGGTACAG TGTCTTGAGC GGTTCCGAGC TGAGGAGAAG 
GGATGGGGGA TCCGGACCAA AGAACCTTTG AAAGCTGGCC AGTTCATTAT TGAATACTTG 
GGAGAAGTTG TCAGTGAGCA GGAGTTCAGA AACCGAATGA TTGAACAGTA CCATAATCAC 
AGTGACCATT ACTGCCTAAA TCTGGACAGT GGAATGGTGA TTGACAGCTA CCGAATGGGC 
AATGAGGCCC GGTTCATCAA CCACAGCTGT AACCCAAATT GTGAAATGCA GAAATGGTCT 
GTTAATGGAG TCTACCGGAT TGGGCTCTAT GCCCTGAAAG ATATGCCAGC TGGTACTGAA 
CTAACCTATG ACTACAATTT TCATTCTTTT AATGTTGAGA AACAGCAACT TTGTAAGTGT 
GGTTTTGACA AATGCCGGGG TATCATCGGA GGCAAGAGCC AACGCATGAA TGGACTCACC 
AGCAAAAGCA GCCAACCTGT GACCACACAC AGACGGCCAG GACGGTCAAA AGAGAAGAGA 
AAATCCAAGC ACAAGTTGAA GAAAAGGAGA GGCCATCTTT CTGAGGAGCC TAATGAAAAT 
GTCAGTACCC CAACCAGAAT GACCCCCCAA CTGCAAATGA AGCCAATGTC CAACCGTGAA 
AGGAACTTTG TGTTAAAACA TCATGTATTC CTGGTCCGGA ACTGGGAGAA AATCAGACAG 
AAGCAAGAGG AGGTAAAACA CACCAGTGAC AATATCCATT CAGCTTCCTT ATATACCCGC 
TGGAATGGTA TCTGCCGAGA TGATGGAAAC ATCAAATCTG ATGTCTTCAT GACCCAGTTC 
TCCGCCTTGC AGACAGCCCG CTCAGTTCGT ACAAGGAGAT TGGCAGCTGC AGAGGAGAAC 
ATTGAAGTGG CCCGGGCAGC CCGCCTGGCC CAGATATTCA AAGAGATATG TGACAGCATC 
ATCTCCTACA AAGATTCCTC CCGACAGGCA TTGGCAACTC CACTGTTGAA CCTTCCCCCA 
AAGAAAAAGA ATGCTGATTA TTATGAGAAG ATATCTGACC CCTTAGATCT CATTACCATT 
GAGAAGCAGA TCCTGACAGG GTATTACAAG ACAGTGGAGG CGTTTGATGC TGACATGCTC 
AAGGTCTTCC GGAATGCTGA GAAATACTAT GGACGCAAGT CCCCAATTGG GAGAGATGTA 
TGTCGACTAC GCAAAGCATA CTACAATGCA CGGCATGAGG CATCAGCTCA AATTGATGAG 
ATTGTGGGGG AAACAGCAAG TGAAGCAGAC AGCAGCGAAA CCTCAGTCTG TGAGAAGGAA 
AATGGGCATG AGAAGGATGA TGATGTTATC CGCTGTATCT GCGGCCTCTA CAAGGATGAA 
GGCCTCATGA TTCAGTGTGA CAAGTGCATG GTGTGGCAGC ACTGTGATTG CATGGGAGTA 
AACTCAGATG TAGAACATTA CCTCTGTGAA CAATGTGATC CAAGGCCTGT GGACAGGGAA 
GTGCCCATGA TACCTCGGCC CCATTATGCC CAACCTGGCT GTGTCTACTT CATCTGTCTA 
CTCCGGGATG ACCTGCTGCT ACGTCAAGGT GACTGTGTGT ACCTGATGCG AGATAGTCGG 
AGAACCCCTG ATGGCCACCC AGTCCGTCAG TCTTATCGAT TGTTATCCCA TATCAACCGA 
GAGAAACTTG ATATCTTTCG AATTGAGAAG CTTTGGAAGA ATGAAAAAGA GGAGCGGTTT 
GCCTTTGGTC ATCATTATTT TCGTCCCCAT GAAACCCACC ACTCCCCATC ACGTCGATTT 
TATCACAATG AGCTGTTCCG AGTGCCACTG TATGAAATCA TTCCTCTGGA GGCTGTGGTG 
GGGACCTGTT GCGTATTAGA TCTGTACACA TATTGTAAAG GAAGGCCCAA AGGAGTGAAA 
GAGCAGGATG TATATATCTG TGACTACCGA CTTGATAAAT CAGCCCACCT GTTCTACAAG 
ATTCATCGGA ACCGCTATCC TGTCTGCACC AAACCCTATG CCTTTGACCA CTTCCCCAAG 
AAGCTCACTC CCAAGAGAGA TTTCTCACCT CATTATGTCC CAGACAACTA CAAGAGGAAT 
GGAGGAAGAT CATCCTGGAA GACAGAACGT TCAAAGCCAC CCATCAAAGA CCTAGGCCAG 
GAGGATGATG CCCTACCACT CATTGAAGAA GTACTGGCCA GTCAAGAGCA AGCAGCCAAT 
GAGATGCCCA CCCTAGAAGA AACTGATCGG GAGGTAGCAC CTGGTGAAAC AACTGAGGGT 
GAAAAGAAAG CTGATGAAAG CAGACAGGAG CCTCAGAGGT CCTGCACACC TGAAGAGAGG 
AGACACAGCC AACGGGAGCG ACTCAACCAG ATTCTGCTCA ACCTTCTTGA GAAGATCCCT 
GGGAAGAATG CCATCGATGT GACATACTTG CTGGAGGAAG GATCAGGAAG AAAGCTTCGA 
AGACGTACTC TGCTTATCCC AGAGAGCAGC TTCCGAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASH1L ( XM_031966722.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031822582.1 Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X4, mRNA.

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CloneID	OSa154248
Clone ID Related Accession (Same CDS sequence)	XM_023502250.1 , XM_031966722.1 , XM_031966719.1 , XM_031966721.1 , XM_031966720.1
Accession Version	XM_031966722.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	histone-lysine N-methyltransferase ASH1L isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATA GGAATACTGC TATGTTAGGA GTGGGTACCG ACTCCGAAGG ATTTTCAAGG 
AAGAGTCCTT CTGCTGTCAG CACTGGAACA CTGGTTAGTA AGAGAGAAGT GGAACTAGAG 
AATAGCACTA AGGAAGAAGA GGATCTTTGG AGGCGGAATA GGGACAGAAA CATTGAGACA 
GGGAAAAATG ATGGTTTGAC TGATGCACAG CAGTTTTCTG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAGTTGAA AATTGGCCTT CAGGCTAAGA GAACTAAAAA GCCTCCTAAA 
AACTTGGAGA ACTATGTATG TCGACCTGCT ATAAAAACAA GCATTAAGCA TCCAAGGAAA 
GCATTGAAAA GTGGGAAGAT GACAGATGAA AAAAATGAAC TTTGCCCTTC AAAACGAACT 
ATGTCGGGAA TATATATCTC CACATTGCAA AACAACATGT ATGTTACACA TTGGAGGAGG 
GGAGAAAAAG ACCCTTCAAA ACTTTACAAG AAAGCAAATG ATGTTGCAGC TGTTGAATGC 
CGCTCTGAAG AAGGCATCCA CCTTCATTCA CAGGGAGAAA ATAATCCTGT GTCAAAGAAG 
CTTTCTCCAC TACACTCTGA AATGACAGAT TATGTTAAAT CAGCACCATC TACTCTTGTT 
GGTAACCGTG ATCCTGACTC GAAGGACAGA GCTCTACTTA ATGGAGCAAC AAGTGTAACA 
GAAAAATTGG CACAGCTCAT TGCTACTTGT CCACCCTCCA AGTCTTCCAA GACAAAGCCA 
AAAAAGTTAG GGGCTGGTAT TACAGCAGGA TTGGTTAGCA AAGACTTGAT CAGAAAGCCA 
GGCATTGGTC CTATGGCAGG AATTATTCAC AAGGACTTGA TTAAGAAACC AACAATTAGC 
CCAGCAGTTG GGTTGGTAAC TAAAGACCCT GGGAAAAAGC CAATCTTTAG TGCTGCAGTA 
GGATTGGTCA GTAAAGACTC TGGGAAAAAA TTAGGAACTG GGACTACTGC GGTATTCATA 
AACAAGGACT TAGGAAAGAA ACCAGGGACT ATCACTACAG TGGGACTGGT GAGCAGAGAG 
TCAGGGAAGA AATTAGGAAT TGGTATCGTC CCAGGTTTGG TGAGTAAAGA ATCAGGAAAG 
AAGTTGGGAC TTGGTACTGT AGTTGGGCTT GTTAATAAAG ATTCAGTAAA GAAGTTTGGC 
CCTGCCATTG GACTGGTTGC AAAGGACTGT GGGAAGAAGA CTATAACAAA TTCATCAGTG 
GGATTGGTTA ATAAGGACCC TGCAAAGAAA TTGGTGGCTT GCCCTGTAGC AGGATTGGTC 
AACAAAGACA TTGTGAATTT AAAAGCTGAA GCCTTGCTCC CTGTTCAAGA ACCACTTAAG 
GTTTCTTGCA ATACAAATAC TAGTAGTCAT GAAAGTCAGG AGCATTCTGA ATCCCTGAAA 
GATAGTGCCA CCAGCAAAGC TTTTGAGAAG ACTATTATTC GTCAGAATAA AGATAGTTTA 
TTAGAAAAGT TCTCAATTCG AAAAGAAATC ATTACTTTGG ACAAAGACAT TTTTAATGAA 
GGAACTTGCA TACAGCAGGA CAGTTTCTTA TCCAGTGAAA GGGGGACCTA TGTTGAAACT 
ACAAAGCATG AGAAGCAGCC TCCAGTGTAT TGCACTTCAC CAGATTTTCA AATGGGAGGC 
ACTTCTGATG CATCAACAGC TAAGTCTCCC TTTAGTGCAA TAGGGGAGAG TAATCTCCCT 
TCCCCTTCAC CAGCCTTGTC TGTTACGCCC TTAAACAGAA GTCCCCCTGG AACTTCTTCT 
CAGTTGGCTA CTAATCCATT ACATTTAGGT CCTTCCACAG AACTCATAGA AGGAATTGCT 
GAATCTATTG GAAAAACCCA ATTCAGTTCT GAAAATTCCC ATATGAACCT TGGTCACAGG 
TCTCTGGTTC ACAGCATGAA CACTGATTGC AAAGGAAATG ATAAAGAGTT AAATGATTCA 
AAAGCTTCTC ACACAGATAA CTCAAGAATG GGCTCCTCCA TAGGAAAAAA GCCAAGTCTG 
GCTTCAGAAT CCAGTATTCA TGCTACAATT ACTCCCTCAG TTGTTAGTTT CACTAGTTTA 
TTTAGCAGTA AACCCTTTCT GAAGATTGGT GCAGTAACTG CATCAGAGAA ACACTGCCAA 
GTTGCTGAAA GCCTGAGCAC TAGTTTGCAG TCCAAACCAT TAAAAAAAAG AAAAGGAAGG 
AAACCACGAT GGACTAAAGT GGTAGCAAGA AACACATGCC GGCCTTCAAA AGGGCTAGAA 
TTAGAAAGGT CAGAGCTTTT TAAAAATGTT TCATGTAGCT CTTTGTCAAA TAGCAATTCC 
GAGCCAGCCA AGTTTATGAA AAACATTGGG CCATCTTCAT TTGTGGAGCA TGACTTCCTT 
AAATGCCGAT TGCCAAAGCT GAGTAAATCT AGCACAGCTC CATCTCTTGC TCTCTTAACT 
GAAACTGACA AGCAGTCTCA TAAGTCCTTT GCCAGCCACA AACTTTCCTC TAGTATATGC 
ATGTCTGGTG ACCTTTTACC TGACATTTAT AAACCTAAAA GAGGAAGACC TAAAACTAAG 
GAGATGCCCC AACTAGAAGG TCCACCCAAA AGGACCCTAA AAATCCCGGC CTCTAAAGTT 
TTTTCATTAC AGTCAAAAGA GGAGCAAGAA CCCCCTATTT TGCAGCCAGA AATTGAAATC 
CCTTCCTTCA AGCAGAGCCT ATCAGTATCA CCCTTTCCCA AAAAAAGAGG AAGGCCAAAA 
AGACAAATCA GATCACCAGT CAAGATGAAA CCACCTGTGC TATCAGTGGC ACCCTTTGTA 
GCCACTGAAA GTCCAAGTAA ACTAGAGTCT GAGAGTGATA AACATAGAAG TAGTAGTGAC 
TTCTTTGAAA GGAAAGATCA ACTTCAGGGT CCAGATGACA GTCATAAACC AAGTGTGTGT 
GGTATGAGTG ACCTTGAGAT GGAACCTGAT AAAAAAATTA ATAAGAGAAA CAATGGCCAA 
TTAATGAAAA CTATCATTCG CAAAATAAAT AAGATGAAGA CTTTGAAGAG AAAGAAACTG 
CTGAATCAGA TTCTCTCAAG TTCTGTTGAA TCAAGTAACA AAGGGAAAGT GCAATCCAAA 
CTCCACAATA CAGTGTCAAC ACTTGCTGCC ACTTTTGGCT CCAAACTGGG CCAACAGATA 
AATGTCAGCA AGAAAGGAAC CATTTATATA GGAAAGAGGC GAGGCCGGAA ACCAAAAGCT 
GTGCTGAACG GCATCTTAAC TAGTAGCCCA GCTAGTCTGG CTGTCCTAGA GAAAACAGCT 
CATCAGGTAG CTGGGTCAGC ATTAGGACAG ATTCTCCCCC CACTCTTGCC TTCAGCAGCA 
AGTAGTTCTG AGGTTCTTCC ATCCCCCATC TGTTCTCAGT CTTCTGGGAC TAGTGGGGGC 
CAGAGTCCTG TCAGCAGTGA TGCAGGATTT GTTGAGCCCA GTTCAGTGCC ATATTTACAT 
ATACACTCCA GACAGGGCAG TATGATTCAG ACACTTGCTA TGAAGAAGGC TTCAAAGGGA 
AGGAGACGGT TGTCTCCTCC TACTTTGTTG CCAAATTCTC CTTCTCACTT GAGTGAACTC 
ACTTCTCTGA AGGAAGCTAC TCCTTCCCCA ATTAGTGAAT CTCATAGTGA TGAGACCATT 
CCTAGTGATA GTGGAATTGG AACAGATAAT AATAGCACAT CAGACAGGGC AGAAAAGTTT 
TGTGGGCAGA AAAAGAGGAG GCATTCTTTT GAACATGTTT CTCTCATTCC CCCTGAAACC 
TCCACAGTCC TAAGTAGTCT CAAAGAGAAA CATAAACACA AATGTAAACG CAGGAGTCAT 
GATTACCTCA GTTATGACAA AATGAAAAGA CAGAAGCGAA AACGGAAAAA GAAGTATCCC 
CAGTTACGAA ACAGACAGGA TCCAGACTTT CTTGCTGAGC TGGAGGAATT AATAAGTCGT 
TTAAGTGAAA TTCGGATCAC TCATCGAAGT CATCATTTTA TTCCCCGTGA TCTCCTACCC 
ACTATTTTTC GAATTAACTT TAACAGTTTC TATACGCACC CCACTTTTCC CTTAGACCCT 
TTGCACTACA TTAGAAAACC TGACTTAAAG AAAAAAAGAG GGAGGCCTCC CAAGATGAGG 
GAAGCAATGG CTGAAATGCC TTTTATGCAT AGCCTTAGTT TCCCTCTCTC TAGTACTGGG 
TTTTACCCCT CTTATGGCAT GCCTTATTCT CCCTCTCCAC TTGCAGCTGC TCCCATAGGA 
TTAGGATACT ATGGAAGGTA CCCTCCAACT CTTTATCCAC CACCCCCCTC TCCTTCTTTC 
ACCACTCCTT TGCCACCTCC TTCTTACATG CATGCTGGTC ATTTGCTTCT CAATCCTGCC 
AAGTACCACA AGAAGAAACA TAAGCTGCTT CGTCAGGAAG CCTTTCTTAC AACAAGTAGG 
ACACCCCTTC TCTCCATGAG CACCTACCCC AGTGTCCCTC CTGAGATGGC CTATGGTTGG 
ATGGTGGAGC ATAAACACCG GCACCGGCAC AAACACAGAG AACACCGTTC TTCTGAGCAA 
CCCCAGGTTT CCATGGACAC TTTAGCTACC AATAGCTCTT CCAGAACTGT CCTAGAATCG 
TTGAAGCGCT ATCGATTTGG TAAAGATGCT GTTGGGGAGC GATATAAACA CAAAGAGAAG 
CATCGTTGTC ACATGTCCTG TCCTCATCTG TCCCCATCAA AAAGCTTATT AAGCCGAGAG 
GAGCAATGGG TCCACCGAGA GCCTTCAGAA TCCACTTCAT TGGCCTTGGG GTTGCAGACA 
CCTTTACAGA TTGACTGTTC AGAAACGTCT CCAGGTTTGT CCCTGGGAGG TTTTACACCT 
AGCTCTGAGC CAGCCAGCAG TGATGAACAC ACAAACCTTT TCACAAGTGC AATAGGCAGC 
TGCAGAGTAT CAAACTCTAC TAATACAAGT GGCCGGAAGA AATTGACTGA CAGTCCAGGA 
CTCTTTACCG CACAGGACAC TTCATTAAAT CGGCCTCTTA GAAAGGAAAC TTTGCCTTCC 
AATGAAAGGG CACTGCAAAC TTTGACAGGC TCCCAGCCAG TCCTAGACAA GCCTTCCCAA 
AGGCCATCAG AGAGCACAAA CTGTAGCCCT ACTCGAAAGA GATCTTCATC AGAGAGCACT 
TCTTCAACAG TGATTGGAAT CCCTTCCCGA GGTACAAGAT TAGGAACATC TGTGGATGAC 
TCTGTGGATA GCCTGCTACA GCGTATGGCG CAACATGAAA GCCAAGGGAC CTTAGAGAAA 
AACATTGATG CTGTCATTGC CACAGTGTCC ATCCCACCTT CCTCAAGCCC AGGTCATACT 
CATGGTAGGG AACGAAGTCT GGGGAAATCA GACAGTCTGC TAGTACCCAC AGCACCTAGT 
GACTCCTGCA GCAACACCAC CACCACCATC TCACTCCTTT CTGAGAGGTT GCCAAGCAGC 
TGTTCCCCCC ATCATCTCAA AAGGAGTGTG GTAGAAGCCA TGCAACGCCA AGCCCGGAAA 
ATGTGCAATT ATAACAAAAT CTTGGCCACC AAGAAAAACC TAGACCATGT CAATAAAATC 
TTAAAAGCCA AAAAGCTTCA AAGACAGGCA AGGACAGGTA ACAATTTTGT GAAGCGCAGA 
CCAGGCCGAC CCCGGAAATA TCCCCTCCAG GCTGTGGTGT CAATGCAGGC ATTCCAGGCT 
GCCCAATTTG CCAGCCCAGA ATTAGATGAA GGGGAGGAAG GCAGAGCACT ACATCTTGGT 
CCTGATACAG TTACAGATGT CATTGAGGCT GTTGTTCAGA GTGTGAACCT GAACCCAGAA 
CTTAGAAAAG GATGGAAAAG GAAAAGTTGG CTTCTGGAGG AAGAGGCAAG GAAAAGACAG 
AAGATACTTC CAGAGGAAGA ACAGGAAAAT AATAAAAGCT TTCCTGAGAC AACAATTGAA 
TCTCCTAGTC CTCCAGAAGC CCCACCAAAG CCACCTGAAC CTGAAAATAC CCTGCAGCCT 
TTGCCTTCTC TTATCCCTCG GGAAAAGAAG GCCCCACGTC CCCCAAAGAA GAAATATCAG 
AAAGCAGGGC TCTATTCTGA TGTATACAAA ACTACAGATC CAAAGAGCAG ACTGATACAA 
TTAAAGAAAG AAAAACTGGA GTATACTCCT GGAGAACATG AATATGGATT ATTCCCAGCC 
CCTATTCATG TTGGAAAGTA TTTGAGACAA AAGAGAATTG ACTTTCAGCT TCCTTATGAC 
ATCCTCTGGC AATGGAAACA CAATCAGCTA TATAAAAAGC CAGATGTCCC ACTATACAAA 
AAAATCCGTT CAAATGTCTA CGTTGATGTC AAACCTCTTT CTGGCTATGA GGCTACCACC 
TGCAACTGTA AAAAACCAGA TGATGGTGAT AGGAAGGGTT GTATGGATGA CTGCCTCAAC 
AGAATGATCT TTGCTGAGTG TTCCCCCAAT ACCTGCCCCT GTGGAGAGCA GTGCTGCAAT 
CAGAGGATAC AAAGGCATGA GTGGGTACAG TGTCTTGAGC GGTTCCGAGC TGAGGAGAAG 
GGATGGGGGA TCCGGACCAA AGAACCTTTG AAAGCTGGCC AGTTCATTAT TGAATACTTG 
GGAGAAGTTG TCAGTGAGCA GGAGTTCAGA AACCGAATGA TTGAACAGTA CCATAATCAC 
AGTGACCATT ACTGCCTAAA TCTGGACAGT GGAATGGTGA TTGACAGCTA CCGAATGGGC 
AATGAGGCCC GGTTCATCAA CCACAGCTGT AACCCAAATT GTGAAATGCA GAAATGGTCT 
GTTAATGGAG TCTACCGGAT TGGGCTCTAT GCCCTGAAAG ATATGCCAGC TGGTACTGAA 
CTAACCTATG ACTACAATTT TCATTCTTTT AATGTTGAGA AACAGCAACT TTGTAAGTGT 
GGTTTTGACA AATGCCGGGG TATCATCGGA GGCAAGAGCC AACGCATGAA TGGACTCACC 
AGCAAAAGCA GCCAACCTGT GACCACACAC AGACGGCCAG GACGGTCAAA AGAGAAGAGA 
AAATCCAAGC ACAAGTTGAA GAAAAGGAGA GGCCATCTTT CTGAGGAGCC TAATGAAAAT 
GTCAGTACCC CAACCAGAAT GACCCCCCAA CTGCAAATGA AGCCAATGTC CAACCGTGAA 
AGGAACTTTG TGTTAAAACA TCATGTATTC CTGGTCCGGA ACTGGGAGAA AATCAGACAG 
AAGCAAGAGG AGGTAAAACA CACCAGTGAC AATATCCATT CAGCTTCCTT ATATACCCGC 
TGGAATGGTA TCTGCCGAGA TGATGGAAAC ATCAAATCTG ATGTCTTCAT GACCCAGTTC 
TCCGCCTTGC AGACAGCCCG CTCAGTTCGT ACAAGGAGAT TGGCAGCTGC AGAGGAGAAC 
ATTGAAGTGG CCCGGGCAGC CCGCCTGGCC CAGATATTCA AAGAGATATG TGACAGCATC 
ATCTCCTACA AAGATTCCTC CCGACAGGCA TTGGCAACTC CACTGTTGAA CCTTCCCCCA 
AAGAAAAAGA ATGCTGATTA TTATGAGAAG ATATCTGACC CCTTAGATCT CATTACCATT 
GAGAAGCAGA TCCTGACAGG GTATTACAAG ACAGTGGAGG CGTTTGATGC TGACATGCTC 
AAGGTCTTCC GGAATGCTGA GAAATACTAT GGACGCAAGT CCCCAATTGG GAGAGATGTA 
TGTCGACTAC GCAAAGCATA CTACAATGCA CGGCATGAGG CATCAGCTCA AATTGATGAG 
ATTGTGGGGG AAACAGCAAG TGAAGCAGAC AGCAGCGAAA CCTCAGTCTG TGAGAAGGAA 
AATGGGCATG AGAAGGATGA TGATGTTATC CGCTGTATCT GCGGCCTCTA CAAGGATGAA 
GGCCTCATGA TTCAGTGTGA CAAGTGCATG GTGTGGCAGC ACTGTGATTG CATGGGAGTA 
AACTCAGATG TAGAACATTA CCTCTGTGAA CAATGTGATC CAAGGCCTGT GGACAGGGAA 
GTGCCCATGA TACCTCGGCC CCATTATGCC CAACCTGGCT GTGTCTACTT CATCTGTCTA 
CTCCGGGATG ACCTGCTGCT ACGTCAAGGT GACTGTGTGT ACCTGATGCG AGATAGTCGG 
AGAACCCCTG ATGGCCACCC AGTCCGTCAG TCTTATCGAT TGTTATCCCA TATCAACCGA 
GAGAAACTTG ATATCTTTCG AATTGAGAAG CTTTGGAAGA ATGAAAAAGA GGAGCGGTTT 
GCCTTTGGTC ATCATTATTT TCGTCCCCAT GAAACCCACC ACTCCCCATC ACGTCGATTT 
TATCACAATG AGCTGTTCCG AGTGCCACTG TATGAAATCA TTCCTCTGGA GGCTGTGGTG 
GGGACCTGTT GCGTATTAGA TCTGTACACA TATTGTAAAG GAAGGCCCAA AGGAGTGAAA 
GAGCAGGATG TATATATCTG TGACTACCGA CTTGATAAAT CAGCCCACCT GTTCTACAAG 
ATTCATCGGA ACCGCTATCC TGTCTGCACC AAACCCTATG CCTTTGACCA CTTCCCCAAG 
AAGCTCACTC CCAAGAGAGA TTTCTCACCT CATTATGTCC CAGACAACTA CAAGAGGAAT 
GGAGGAAGAT CATCCTGGAA GACAGAACGT TCAAAGCCAC CCATCAAAGA CCTAGGCCAG 
GAGGATGATG CCCTACCACT CATTGAAGAA GTACTGGCCA GTCAAGAGCA AGCAGCCAAT 
GAGATGCCCA CCCTAGAAGA AACTGATCGG GAGGTAGCAC CTGGTGAAAC AACTGAGGGT 
GAAAAGAAAG CTGATGAAAG CAGACAGGAG CCTCAGAGGT CCTGCACACC TGAAGAGAGG 
AGACACAGCC AACGGGAGCG ACTCAACCAG ATTCTGCTCA ACCTTCTTGA GAAGATCCCT 
GGGAAGAATG CCATCGATGT GACATACTTG CTGGAGGAAG GATCAGGAAG AAAGCTTCGA 
AGACGTACTC TGCTTATCCC AGAGAGCAGC TTCCGAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031822582.1
CDS	150..9131
Translation	MDHRNTAMLGVGTDSEGFSRKSPSAVSTGTLVSKREVELENSTKEEEDLWRRNRDRNIETGKNDGLTDAQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTSIKHPRKALKSGKMTDEKNELCPSKRTMSGIYISTLQNNMYVTHWRRGEKDPSKLYKKANDVAAVECRSEEGIHLHSQGENNPVSKKLSPLHSEMTDYVKSAPSTLVGNRDPDSKDRALLNGATSVTEKLAQLIATCPPSKSSKTKPKKLGAGITAGLVSKDLIRKPGIGPMAGIIHKDLIKKPTISPAVGLVTKDPGKKPIFSAAVGLVSKDSGKKLGTGTTAVFINKDLGKKPGTITTVGLVSRESGKKLGIGIVPGLVSKESGKKLGLGTVVGLVNKDSVKKFGPAIGLVAKDCGKKTITNSSVGLVNKDPAKKLVACPVAGLVNKDIVNLKAEALLPVQEPLKVSCNTNTSSHESQEHSESLKDSATSKAFEKTIIRQNKDSLLEKFSIRKEIITLDKDIFNEGTCIQQDSFLSSERGTYVETTKHEKQPPVYCTSPDFQMGGTSDASTAKSPFSAIGESNLPSPSPALSVTPLNRSPPGTSSQLATNPLHLGPSTELIEGIAESIGKTQFSSENSHMNLGHRSLVHSMNTDCKGNDKELNDSKASHTDNSRMGSSIGKKPSLASESSIHATITPSVVSFTSLFSSKPFLKIGAVTASEKHCQVAESLSTSLQSKPLKKRKGRKPRWTKVVARNTCRPSKGLELERSELFKNVSCSSLSNSNSEPAKFMKNIGPSSFVEHDFLKCRLPKLSKSSTAPSLALLTETDKQSHKSFASHKLSSSICMSGDLLPDIYKPKRGRPKTKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQIRSPVKMKPPVLSVAPFVATESPSKLESESDKHRSSSDFFERKDQLQGPDDSHKPSVCGMSDLEMEPDKKINKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSTLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKAVLNGILTSSPASLAVLEKTAHQVAGSALGQILPPLLPSAASSSEVLPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHIHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFLAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPTFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLAAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPAKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDTLATNSSSRTVLESLKRYRFGKDAVGERYKHKEKHRCHMSCPHLSPSKSLLSREEQWVHREPSESTSLALGLQTPLQIDCSETSPGLSLGGFTPSSEPASSDEHTNLFTSAIGSCRVSNSTNTSGRKKLTDSPGLFTAQDTSLNRPLRKETLPSNERALQTLTGSQPVLDKPSQRPSESTNCSPTRKRSSSESTSSTVIGIPSRGTRLGTSVDDSVDSLLQRMAQHESQGTLEKNIDAVIATVSIPPSSSPGHTHGRERSLGKSDSLLVPTAPSDSCSNTTTTISLLSERLPSSCSPHHLKRSVVEAMQRQARKMCNYNKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKYPLQAVVSMQAFQAAQFASPELDEGEEGRALHLGPDTVTDVIEAVVQSVNLNPELRKGWKRKSWLLEEEARKRQKILPEEEQENNKSFPETTIESPSPPEAPPKPPEPENTLQPLPSLIPREKKAPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPDDGDRKGCMDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCNPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFDKCRGIIGGKSQRMNGLTSKSSQPVTTHRRPGRSKEKRKSKHKLKKRRGHLSEEPNENVSTPTRMTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIRQKQEEVKHTSDNIHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENIEVARAARLAQIFKEICDSIISYKDSSRQALATPLLNLPPKKKNADYYEKISDPLDLITIEKQILTGYYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKAYYNARHEASAQIDEIVGETASEADSSETSVCEKENGHEKDDDVIRCICGLYKDEGLMIQCDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINREKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKTERSKPPIKDLGQEDDALPLIEEVLASQEQAANEMPTLEETDREVAPGETTEGEKKADESRQEPQRSCTPEERRHSQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLLIPESSFRK

Target ORF information:

RefSeq Version	XM_031966722.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031966722.1

ORF Insert Sequence:

ATGGACCATA GGAATACTGC TATGTTAGGA GTGGGTACCG ACTCCGAAGG ATTTTCAAGG 
AAGAGTCCTT CTGCTGTCAG CACTGGAACA CTGGTTAGTA AGAGAGAAGT GGAACTAGAG 
AATAGCACTA AGGAAGAAGA GGATCTTTGG AGGCGGAATA GGGACAGAAA CATTGAGACA 
GGGAAAAATG ATGGTTTGAC TGATGCACAG CAGTTTTCTG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAGTTGAA AATTGGCCTT CAGGCTAAGA GAACTAAAAA GCCTCCTAAA 
AACTTGGAGA ACTATGTATG TCGACCTGCT ATAAAAACAA GCATTAAGCA TCCAAGGAAA 
GCATTGAAAA GTGGGAAGAT GACAGATGAA AAAAATGAAC TTTGCCCTTC AAAACGAACT 
ATGTCGGGAA TATATATCTC CACATTGCAA AACAACATGT ATGTTACACA TTGGAGGAGG 
GGAGAAAAAG ACCCTTCAAA ACTTTACAAG AAAGCAAATG ATGTTGCAGC TGTTGAATGC 
CGCTCTGAAG AAGGCATCCA CCTTCATTCA CAGGGAGAAA ATAATCCTGT GTCAAAGAAG 
CTTTCTCCAC TACACTCTGA AATGACAGAT TATGTTAAAT CAGCACCATC TACTCTTGTT 
GGTAACCGTG ATCCTGACTC GAAGGACAGA GCTCTACTTA ATGGAGCAAC AAGTGTAACA 
GAAAAATTGG CACAGCTCAT TGCTACTTGT CCACCCTCCA AGTCTTCCAA GACAAAGCCA 
AAAAAGTTAG GGGCTGGTAT TACAGCAGGA TTGGTTAGCA AAGACTTGAT CAGAAAGCCA 
GGCATTGGTC CTATGGCAGG AATTATTCAC AAGGACTTGA TTAAGAAACC AACAATTAGC 
CCAGCAGTTG GGTTGGTAAC TAAAGACCCT GGGAAAAAGC CAATCTTTAG TGCTGCAGTA 
GGATTGGTCA GTAAAGACTC TGGGAAAAAA TTAGGAACTG GGACTACTGC GGTATTCATA 
AACAAGGACT TAGGAAAGAA ACCAGGGACT ATCACTACAG TGGGACTGGT GAGCAGAGAG 
TCAGGGAAGA AATTAGGAAT TGGTATCGTC CCAGGTTTGG TGAGTAAAGA ATCAGGAAAG 
AAGTTGGGAC TTGGTACTGT AGTTGGGCTT GTTAATAAAG ATTCAGTAAA GAAGTTTGGC 
CCTGCCATTG GACTGGTTGC AAAGGACTGT GGGAAGAAGA CTATAACAAA TTCATCAGTG 
GGATTGGTTA ATAAGGACCC TGCAAAGAAA TTGGTGGCTT GCCCTGTAGC AGGATTGGTC 
AACAAAGACA TTGTGAATTT AAAAGCTGAA GCCTTGCTCC CTGTTCAAGA ACCACTTAAG 
GTTTCTTGCA ATACAAATAC TAGTAGTCAT GAAAGTCAGG AGCATTCTGA ATCCCTGAAA 
GATAGTGCCA CCAGCAAAGC TTTTGAGAAG ACTATTATTC GTCAGAATAA AGATAGTTTA 
TTAGAAAAGT TCTCAATTCG AAAAGAAATC ATTACTTTGG ACAAAGACAT TTTTAATGAA 
GGAACTTGCA TACAGCAGGA CAGTTTCTTA TCCAGTGAAA GGGGGACCTA TGTTGAAACT 
ACAAAGCATG AGAAGCAGCC TCCAGTGTAT TGCACTTCAC CAGATTTTCA AATGGGAGGC 
ACTTCTGATG CATCAACAGC TAAGTCTCCC TTTAGTGCAA TAGGGGAGAG TAATCTCCCT 
TCCCCTTCAC CAGCCTTGTC TGTTACGCCC TTAAACAGAA GTCCCCCTGG AACTTCTTCT 
CAGTTGGCTA CTAATCCATT ACATTTAGGT CCTTCCACAG AACTCATAGA AGGAATTGCT 
GAATCTATTG GAAAAACCCA ATTCAGTTCT GAAAATTCCC ATATGAACCT TGGTCACAGG 
TCTCTGGTTC ACAGCATGAA CACTGATTGC AAAGGAAATG ATAAAGAGTT AAATGATTCA 
AAAGCTTCTC ACACAGATAA CTCAAGAATG GGCTCCTCCA TAGGAAAAAA GCCAAGTCTG 
GCTTCAGAAT CCAGTATTCA TGCTACAATT ACTCCCTCAG TTGTTAGTTT CACTAGTTTA 
TTTAGCAGTA AACCCTTTCT GAAGATTGGT GCAGTAACTG CATCAGAGAA ACACTGCCAA 
GTTGCTGAAA GCCTGAGCAC TAGTTTGCAG TCCAAACCAT TAAAAAAAAG AAAAGGAAGG 
AAACCACGAT GGACTAAAGT GGTAGCAAGA AACACATGCC GGCCTTCAAA AGGGCTAGAA 
TTAGAAAGGT CAGAGCTTTT TAAAAATGTT TCATGTAGCT CTTTGTCAAA TAGCAATTCC 
GAGCCAGCCA AGTTTATGAA AAACATTGGG CCATCTTCAT TTGTGGAGCA TGACTTCCTT 
AAATGCCGAT TGCCAAAGCT GAGTAAATCT AGCACAGCTC CATCTCTTGC TCTCTTAACT 
GAAACTGACA AGCAGTCTCA TAAGTCCTTT GCCAGCCACA AACTTTCCTC TAGTATATGC 
ATGTCTGGTG ACCTTTTACC TGACATTTAT AAACCTAAAA GAGGAAGACC TAAAACTAAG 
GAGATGCCCC AACTAGAAGG TCCACCCAAA AGGACCCTAA AAATCCCGGC CTCTAAAGTT 
TTTTCATTAC AGTCAAAAGA GGAGCAAGAA CCCCCTATTT TGCAGCCAGA AATTGAAATC 
CCTTCCTTCA AGCAGAGCCT ATCAGTATCA CCCTTTCCCA AAAAAAGAGG AAGGCCAAAA 
AGACAAATCA GATCACCAGT CAAGATGAAA CCACCTGTGC TATCAGTGGC ACCCTTTGTA 
GCCACTGAAA GTCCAAGTAA ACTAGAGTCT GAGAGTGATA AACATAGAAG TAGTAGTGAC 
TTCTTTGAAA GGAAAGATCA ACTTCAGGGT CCAGATGACA GTCATAAACC AAGTGTGTGT 
GGTATGAGTG ACCTTGAGAT GGAACCTGAT AAAAAAATTA ATAAGAGAAA CAATGGCCAA 
TTAATGAAAA CTATCATTCG CAAAATAAAT AAGATGAAGA CTTTGAAGAG AAAGAAACTG 
CTGAATCAGA TTCTCTCAAG TTCTGTTGAA TCAAGTAACA AAGGGAAAGT GCAATCCAAA 
CTCCACAATA CAGTGTCAAC ACTTGCTGCC ACTTTTGGCT CCAAACTGGG CCAACAGATA 
AATGTCAGCA AGAAAGGAAC CATTTATATA GGAAAGAGGC GAGGCCGGAA ACCAAAAGCT 
GTGCTGAACG GCATCTTAAC TAGTAGCCCA GCTAGTCTGG CTGTCCTAGA GAAAACAGCT 
CATCAGGTAG CTGGGTCAGC ATTAGGACAG ATTCTCCCCC CACTCTTGCC TTCAGCAGCA 
AGTAGTTCTG AGGTTCTTCC ATCCCCCATC TGTTCTCAGT CTTCTGGGAC TAGTGGGGGC 
CAGAGTCCTG TCAGCAGTGA TGCAGGATTT GTTGAGCCCA GTTCAGTGCC ATATTTACAT 
ATACACTCCA GACAGGGCAG TATGATTCAG ACACTTGCTA TGAAGAAGGC TTCAAAGGGA 
AGGAGACGGT TGTCTCCTCC TACTTTGTTG CCAAATTCTC CTTCTCACTT GAGTGAACTC 
ACTTCTCTGA AGGAAGCTAC TCCTTCCCCA ATTAGTGAAT CTCATAGTGA TGAGACCATT 
CCTAGTGATA GTGGAATTGG AACAGATAAT AATAGCACAT CAGACAGGGC AGAAAAGTTT 
TGTGGGCAGA AAAAGAGGAG GCATTCTTTT GAACATGTTT CTCTCATTCC CCCTGAAACC 
TCCACAGTCC TAAGTAGTCT CAAAGAGAAA CATAAACACA AATGTAAACG CAGGAGTCAT 
GATTACCTCA GTTATGACAA AATGAAAAGA CAGAAGCGAA AACGGAAAAA GAAGTATCCC 
CAGTTACGAA ACAGACAGGA TCCAGACTTT CTTGCTGAGC TGGAGGAATT AATAAGTCGT 
TTAAGTGAAA TTCGGATCAC TCATCGAAGT CATCATTTTA TTCCCCGTGA TCTCCTACCC 
ACTATTTTTC GAATTAACTT TAACAGTTTC TATACGCACC CCACTTTTCC CTTAGACCCT 
TTGCACTACA TTAGAAAACC TGACTTAAAG AAAAAAAGAG GGAGGCCTCC CAAGATGAGG 
GAAGCAATGG CTGAAATGCC TTTTATGCAT AGCCTTAGTT TCCCTCTCTC TAGTACTGGG 
TTTTACCCCT CTTATGGCAT GCCTTATTCT CCCTCTCCAC TTGCAGCTGC TCCCATAGGA 
TTAGGATACT ATGGAAGGTA CCCTCCAACT CTTTATCCAC CACCCCCCTC TCCTTCTTTC 
ACCACTCCTT TGCCACCTCC TTCTTACATG CATGCTGGTC ATTTGCTTCT CAATCCTGCC 
AAGTACCACA AGAAGAAACA TAAGCTGCTT CGTCAGGAAG CCTTTCTTAC AACAAGTAGG 
ACACCCCTTC TCTCCATGAG CACCTACCCC AGTGTCCCTC CTGAGATGGC CTATGGTTGG 
ATGGTGGAGC ATAAACACCG GCACCGGCAC AAACACAGAG AACACCGTTC TTCTGAGCAA 
CCCCAGGTTT CCATGGACAC TTTAGCTACC AATAGCTCTT CCAGAACTGT CCTAGAATCG 
TTGAAGCGCT ATCGATTTGG TAAAGATGCT GTTGGGGAGC GATATAAACA CAAAGAGAAG 
CATCGTTGTC ACATGTCCTG TCCTCATCTG TCCCCATCAA AAAGCTTATT AAGCCGAGAG 
GAGCAATGGG TCCACCGAGA GCCTTCAGAA TCCACTTCAT TGGCCTTGGG GTTGCAGACA 
CCTTTACAGA TTGACTGTTC AGAAACGTCT CCAGGTTTGT CCCTGGGAGG TTTTACACCT 
AGCTCTGAGC CAGCCAGCAG TGATGAACAC ACAAACCTTT TCACAAGTGC AATAGGCAGC 
TGCAGAGTAT CAAACTCTAC TAATACAAGT GGCCGGAAGA AATTGACTGA CAGTCCAGGA 
CTCTTTACCG CACAGGACAC TTCATTAAAT CGGCCTCTTA GAAAGGAAAC TTTGCCTTCC 
AATGAAAGGG CACTGCAAAC TTTGACAGGC TCCCAGCCAG TCCTAGACAA GCCTTCCCAA 
AGGCCATCAG AGAGCACAAA CTGTAGCCCT ACTCGAAAGA GATCTTCATC AGAGAGCACT 
TCTTCAACAG TGATTGGAAT CCCTTCCCGA GGTACAAGAT TAGGAACATC TGTGGATGAC 
TCTGTGGATA GCCTGCTACA GCGTATGGCG CAACATGAAA GCCAAGGGAC CTTAGAGAAA 
AACATTGATG CTGTCATTGC CACAGTGTCC ATCCCACCTT CCTCAAGCCC AGGTCATACT 
CATGGTAGGG AACGAAGTCT GGGGAAATCA GACAGTCTGC TAGTACCCAC AGCACCTAGT 
GACTCCTGCA GCAACACCAC CACCACCATC TCACTCCTTT CTGAGAGGTT GCCAAGCAGC 
TGTTCCCCCC ATCATCTCAA AAGGAGTGTG GTAGAAGCCA TGCAACGCCA AGCCCGGAAA 
ATGTGCAATT ATAACAAAAT CTTGGCCACC AAGAAAAACC TAGACCATGT CAATAAAATC 
TTAAAAGCCA AAAAGCTTCA AAGACAGGCA AGGACAGGTA ACAATTTTGT GAAGCGCAGA 
CCAGGCCGAC CCCGGAAATA TCCCCTCCAG GCTGTGGTGT CAATGCAGGC ATTCCAGGCT 
GCCCAATTTG CCAGCCCAGA ATTAGATGAA GGGGAGGAAG GCAGAGCACT ACATCTTGGT 
CCTGATACAG TTACAGATGT CATTGAGGCT GTTGTTCAGA GTGTGAACCT GAACCCAGAA 
CTTAGAAAAG GATGGAAAAG GAAAAGTTGG CTTCTGGAGG AAGAGGCAAG GAAAAGACAG 
AAGATACTTC CAGAGGAAGA ACAGGAAAAT AATAAAAGCT TTCCTGAGAC AACAATTGAA 
TCTCCTAGTC CTCCAGAAGC CCCACCAAAG CCACCTGAAC CTGAAAATAC CCTGCAGCCT 
TTGCCTTCTC TTATCCCTCG GGAAAAGAAG GCCCCACGTC CCCCAAAGAA GAAATATCAG 
AAAGCAGGGC TCTATTCTGA TGTATACAAA ACTACAGATC CAAAGAGCAG ACTGATACAA 
TTAAAGAAAG AAAAACTGGA GTATACTCCT GGAGAACATG AATATGGATT ATTCCCAGCC 
CCTATTCATG TTGGAAAGTA TTTGAGACAA AAGAGAATTG ACTTTCAGCT TCCTTATGAC 
ATCCTCTGGC AATGGAAACA CAATCAGCTA TATAAAAAGC CAGATGTCCC ACTATACAAA 
AAAATCCGTT CAAATGTCTA CGTTGATGTC AAACCTCTTT CTGGCTATGA GGCTACCACC 
TGCAACTGTA AAAAACCAGA TGATGGTGAT AGGAAGGGTT GTATGGATGA CTGCCTCAAC 
AGAATGATCT TTGCTGAGTG TTCCCCCAAT ACCTGCCCCT GTGGAGAGCA GTGCTGCAAT 
CAGAGGATAC AAAGGCATGA GTGGGTACAG TGTCTTGAGC GGTTCCGAGC TGAGGAGAAG 
GGATGGGGGA TCCGGACCAA AGAACCTTTG AAAGCTGGCC AGTTCATTAT TGAATACTTG 
GGAGAAGTTG TCAGTGAGCA GGAGTTCAGA AACCGAATGA TTGAACAGTA CCATAATCAC 
AGTGACCATT ACTGCCTAAA TCTGGACAGT GGAATGGTGA TTGACAGCTA CCGAATGGGC 
AATGAGGCCC GGTTCATCAA CCACAGCTGT AACCCAAATT GTGAAATGCA GAAATGGTCT 
GTTAATGGAG TCTACCGGAT TGGGCTCTAT GCCCTGAAAG ATATGCCAGC TGGTACTGAA 
CTAACCTATG ACTACAATTT TCATTCTTTT AATGTTGAGA AACAGCAACT TTGTAAGTGT 
GGTTTTGACA AATGCCGGGG TATCATCGGA GGCAAGAGCC AACGCATGAA TGGACTCACC 
AGCAAAAGCA GCCAACCTGT GACCACACAC AGACGGCCAG GACGGTCAAA AGAGAAGAGA 
AAATCCAAGC ACAAGTTGAA GAAAAGGAGA GGCCATCTTT CTGAGGAGCC TAATGAAAAT 
GTCAGTACCC CAACCAGAAT GACCCCCCAA CTGCAAATGA AGCCAATGTC CAACCGTGAA 
AGGAACTTTG TGTTAAAACA TCATGTATTC CTGGTCCGGA ACTGGGAGAA AATCAGACAG 
AAGCAAGAGG AGGTAAAACA CACCAGTGAC AATATCCATT CAGCTTCCTT ATATACCCGC 
TGGAATGGTA TCTGCCGAGA TGATGGAAAC ATCAAATCTG ATGTCTTCAT GACCCAGTTC 
TCCGCCTTGC AGACAGCCCG CTCAGTTCGT ACAAGGAGAT TGGCAGCTGC AGAGGAGAAC 
ATTGAAGTGG CCCGGGCAGC CCGCCTGGCC CAGATATTCA AAGAGATATG TGACAGCATC 
ATCTCCTACA AAGATTCCTC CCGACAGGCA TTGGCAACTC CACTGTTGAA CCTTCCCCCA 
AAGAAAAAGA ATGCTGATTA TTATGAGAAG ATATCTGACC CCTTAGATCT CATTACCATT 
GAGAAGCAGA TCCTGACAGG GTATTACAAG ACAGTGGAGG CGTTTGATGC TGACATGCTC 
AAGGTCTTCC GGAATGCTGA GAAATACTAT GGACGCAAGT CCCCAATTGG GAGAGATGTA 
TGTCGACTAC GCAAAGCATA CTACAATGCA CGGCATGAGG CATCAGCTCA AATTGATGAG 
ATTGTGGGGG AAACAGCAAG TGAAGCAGAC AGCAGCGAAA CCTCAGTCTG TGAGAAGGAA 
AATGGGCATG AGAAGGATGA TGATGTTATC CGCTGTATCT GCGGCCTCTA CAAGGATGAA 
GGCCTCATGA TTCAGTGTGA CAAGTGCATG GTGTGGCAGC ACTGTGATTG CATGGGAGTA 
AACTCAGATG TAGAACATTA CCTCTGTGAA CAATGTGATC CAAGGCCTGT GGACAGGGAA 
GTGCCCATGA TACCTCGGCC CCATTATGCC CAACCTGGCT GTGTCTACTT CATCTGTCTA 
CTCCGGGATG ACCTGCTGCT ACGTCAAGGT GACTGTGTGT ACCTGATGCG AGATAGTCGG 
AGAACCCCTG ATGGCCACCC AGTCCGTCAG TCTTATCGAT TGTTATCCCA TATCAACCGA 
GAGAAACTTG ATATCTTTCG AATTGAGAAG CTTTGGAAGA ATGAAAAAGA GGAGCGGTTT 
GCCTTTGGTC ATCATTATTT TCGTCCCCAT GAAACCCACC ACTCCCCATC ACGTCGATTT 
TATCACAATG AGCTGTTCCG AGTGCCACTG TATGAAATCA TTCCTCTGGA GGCTGTGGTG 
GGGACCTGTT GCGTATTAGA TCTGTACACA TATTGTAAAG GAAGGCCCAA AGGAGTGAAA 
GAGCAGGATG TATATATCTG TGACTACCGA CTTGATAAAT CAGCCCACCT GTTCTACAAG 
ATTCATCGGA ACCGCTATCC TGTCTGCACC AAACCCTATG CCTTTGACCA CTTCCCCAAG 
AAGCTCACTC CCAAGAGAGA TTTCTCACCT CATTATGTCC CAGACAACTA CAAGAGGAAT 
GGAGGAAGAT CATCCTGGAA GACAGAACGT TCAAAGCCAC CCATCAAAGA CCTAGGCCAG 
GAGGATGATG CCCTACCACT CATTGAAGAA GTACTGGCCA GTCAAGAGCA AGCAGCCAAT 
GAGATGCCCA CCCTAGAAGA AACTGATCGG GAGGTAGCAC CTGGTGAAAC AACTGAGGGT 
GAAAAGAAAG CTGATGAAAG CAGACAGGAG CCTCAGAGGT CCTGCACACC TGAAGAGAGG 
AGACACAGCC AACGGGAGCG ACTCAACCAG ATTCTGCTCA ACCTTCTTGA GAAGATCCCT 
GGGAAGAATG CCATCGATGT GACATACTTG CTGGAGGAAG GATCAGGAAG AAAGCTTCGA 
AGACGTACTC TGCTTATCCC AGAGAGCAGC TTCCGAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASH1L ( XM_031966719.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031822579.1 Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X1, mRNA.

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CloneID	OSa154248
Clone ID Related Accession (Same CDS sequence)	XM_023502250.1 , XM_031966722.1 , XM_031966719.1 , XM_031966721.1 , XM_031966720.1
Accession Version	XM_031966719.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	histone-lysine N-methyltransferase ASH1L isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATA GGAATACTGC TATGTTAGGA GTGGGTACCG ACTCCGAAGG ATTTTCAAGG 
AAGAGTCCTT CTGCTGTCAG CACTGGAACA CTGGTTAGTA AGAGAGAAGT GGAACTAGAG 
AATAGCACTA AGGAAGAAGA GGATCTTTGG AGGCGGAATA GGGACAGAAA CATTGAGACA 
GGGAAAAATG ATGGTTTGAC TGATGCACAG CAGTTTTCTG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAGTTGAA AATTGGCCTT CAGGCTAAGA GAACTAAAAA GCCTCCTAAA 
AACTTGGAGA ACTATGTATG TCGACCTGCT ATAAAAACAA GCATTAAGCA TCCAAGGAAA 
GCATTGAAAA GTGGGAAGAT GACAGATGAA AAAAATGAAC TTTGCCCTTC AAAACGAACT 
ATGTCGGGAA TATATATCTC CACATTGCAA AACAACATGT ATGTTACACA TTGGAGGAGG 
GGAGAAAAAG ACCCTTCAAA ACTTTACAAG AAAGCAAATG ATGTTGCAGC TGTTGAATGC 
CGCTCTGAAG AAGGCATCCA CCTTCATTCA CAGGGAGAAA ATAATCCTGT GTCAAAGAAG 
CTTTCTCCAC TACACTCTGA AATGACAGAT TATGTTAAAT CAGCACCATC TACTCTTGTT 
GGTAACCGTG ATCCTGACTC GAAGGACAGA GCTCTACTTA ATGGAGCAAC AAGTGTAACA 
GAAAAATTGG CACAGCTCAT TGCTACTTGT CCACCCTCCA AGTCTTCCAA GACAAAGCCA 
AAAAAGTTAG GGGCTGGTAT TACAGCAGGA TTGGTTAGCA AAGACTTGAT CAGAAAGCCA 
GGCATTGGTC CTATGGCAGG AATTATTCAC AAGGACTTGA TTAAGAAACC AACAATTAGC 
CCAGCAGTTG GGTTGGTAAC TAAAGACCCT GGGAAAAAGC CAATCTTTAG TGCTGCAGTA 
GGATTGGTCA GTAAAGACTC TGGGAAAAAA TTAGGAACTG GGACTACTGC GGTATTCATA 
AACAAGGACT TAGGAAAGAA ACCAGGGACT ATCACTACAG TGGGACTGGT GAGCAGAGAG 
TCAGGGAAGA AATTAGGAAT TGGTATCGTC CCAGGTTTGG TGAGTAAAGA ATCAGGAAAG 
AAGTTGGGAC TTGGTACTGT AGTTGGGCTT GTTAATAAAG ATTCAGTAAA GAAGTTTGGC 
CCTGCCATTG GACTGGTTGC AAAGGACTGT GGGAAGAAGA CTATAACAAA TTCATCAGTG 
GGATTGGTTA ATAAGGACCC TGCAAAGAAA TTGGTGGCTT GCCCTGTAGC AGGATTGGTC 
AACAAAGACA TTGTGAATTT AAAAGCTGAA GCCTTGCTCC CTGTTCAAGA ACCACTTAAG 
GTTTCTTGCA ATACAAATAC TAGTAGTCAT GAAAGTCAGG AGCATTCTGA ATCCCTGAAA 
GATAGTGCCA CCAGCAAAGC TTTTGAGAAG ACTATTATTC GTCAGAATAA AGATAGTTTA 
TTAGAAAAGT TCTCAATTCG AAAAGAAATC ATTACTTTGG ACAAAGACAT TTTTAATGAA 
GGAACTTGCA TACAGCAGGA CAGTTTCTTA TCCAGTGAAA GGGGGACCTA TGTTGAAACT 
ACAAAGCATG AGAAGCAGCC TCCAGTGTAT TGCACTTCAC CAGATTTTCA AATGGGAGGC 
ACTTCTGATG CATCAACAGC TAAGTCTCCC TTTAGTGCAA TAGGGGAGAG TAATCTCCCT 
TCCCCTTCAC CAGCCTTGTC TGTTACGCCC TTAAACAGAA GTCCCCCTGG AACTTCTTCT 
CAGTTGGCTA CTAATCCATT ACATTTAGGT CCTTCCACAG AACTCATAGA AGGAATTGCT 
GAATCTATTG GAAAAACCCA ATTCAGTTCT GAAAATTCCC ATATGAACCT TGGTCACAGG 
TCTCTGGTTC ACAGCATGAA CACTGATTGC AAAGGAAATG ATAAAGAGTT AAATGATTCA 
AAAGCTTCTC ACACAGATAA CTCAAGAATG GGCTCCTCCA TAGGAAAAAA GCCAAGTCTG 
GCTTCAGAAT CCAGTATTCA TGCTACAATT ACTCCCTCAG TTGTTAGTTT CACTAGTTTA 
TTTAGCAGTA AACCCTTTCT GAAGATTGGT GCAGTAACTG CATCAGAGAA ACACTGCCAA 
GTTGCTGAAA GCCTGAGCAC TAGTTTGCAG TCCAAACCAT TAAAAAAAAG AAAAGGAAGG 
AAACCACGAT GGACTAAAGT GGTAGCAAGA AACACATGCC GGCCTTCAAA AGGGCTAGAA 
TTAGAAAGGT CAGAGCTTTT TAAAAATGTT TCATGTAGCT CTTTGTCAAA TAGCAATTCC 
GAGCCAGCCA AGTTTATGAA AAACATTGGG CCATCTTCAT TTGTGGAGCA TGACTTCCTT 
AAATGCCGAT TGCCAAAGCT GAGTAAATCT AGCACAGCTC CATCTCTTGC TCTCTTAACT 
GAAACTGACA AGCAGTCTCA TAAGTCCTTT GCCAGCCACA AACTTTCCTC TAGTATATGC 
ATGTCTGGTG ACCTTTTACC TGACATTTAT AAACCTAAAA GAGGAAGACC TAAAACTAAG 
GAGATGCCCC AACTAGAAGG TCCACCCAAA AGGACCCTAA AAATCCCGGC CTCTAAAGTT 
TTTTCATTAC AGTCAAAAGA GGAGCAAGAA CCCCCTATTT TGCAGCCAGA AATTGAAATC 
CCTTCCTTCA AGCAGAGCCT ATCAGTATCA CCCTTTCCCA AAAAAAGAGG AAGGCCAAAA 
AGACAAATCA GATCACCAGT CAAGATGAAA CCACCTGTGC TATCAGTGGC ACCCTTTGTA 
GCCACTGAAA GTCCAAGTAA ACTAGAGTCT GAGAGTGATA AACATAGAAG TAGTAGTGAC 
TTCTTTGAAA GGAAAGATCA ACTTCAGGGT CCAGATGACA GTCATAAACC AAGTGTGTGT 
GGTATGAGTG ACCTTGAGAT GGAACCTGAT AAAAAAATTA ATAAGAGAAA CAATGGCCAA 
TTAATGAAAA CTATCATTCG CAAAATAAAT AAGATGAAGA CTTTGAAGAG AAAGAAACTG 
CTGAATCAGA TTCTCTCAAG TTCTGTTGAA TCAAGTAACA AAGGGAAAGT GCAATCCAAA 
CTCCACAATA CAGTGTCAAC ACTTGCTGCC ACTTTTGGCT CCAAACTGGG CCAACAGATA 
AATGTCAGCA AGAAAGGAAC CATTTATATA GGAAAGAGGC GAGGCCGGAA ACCAAAAGCT 
GTGCTGAACG GCATCTTAAC TAGTAGCCCA GCTAGTCTGG CTGTCCTAGA GAAAACAGCT 
CATCAGGTAG CTGGGTCAGC ATTAGGACAG ATTCTCCCCC CACTCTTGCC TTCAGCAGCA 
AGTAGTTCTG AGGTTCTTCC ATCCCCCATC TGTTCTCAGT CTTCTGGGAC TAGTGGGGGC 
CAGAGTCCTG TCAGCAGTGA TGCAGGATTT GTTGAGCCCA GTTCAGTGCC ATATTTACAT 
ATACACTCCA GACAGGGCAG TATGATTCAG ACACTTGCTA TGAAGAAGGC TTCAAAGGGA 
AGGAGACGGT TGTCTCCTCC TACTTTGTTG CCAAATTCTC CTTCTCACTT GAGTGAACTC 
ACTTCTCTGA AGGAAGCTAC TCCTTCCCCA ATTAGTGAAT CTCATAGTGA TGAGACCATT 
CCTAGTGATA GTGGAATTGG AACAGATAAT AATAGCACAT CAGACAGGGC AGAAAAGTTT 
TGTGGGCAGA AAAAGAGGAG GCATTCTTTT GAACATGTTT CTCTCATTCC CCCTGAAACC 
TCCACAGTCC TAAGTAGTCT CAAAGAGAAA CATAAACACA AATGTAAACG CAGGAGTCAT 
GATTACCTCA GTTATGACAA AATGAAAAGA CAGAAGCGAA AACGGAAAAA GAAGTATCCC 
CAGTTACGAA ACAGACAGGA TCCAGACTTT CTTGCTGAGC TGGAGGAATT AATAAGTCGT 
TTAAGTGAAA TTCGGATCAC TCATCGAAGT CATCATTTTA TTCCCCGTGA TCTCCTACCC 
ACTATTTTTC GAATTAACTT TAACAGTTTC TATACGCACC CCACTTTTCC CTTAGACCCT 
TTGCACTACA TTAGAAAACC TGACTTAAAG AAAAAAAGAG GGAGGCCTCC CAAGATGAGG 
GAAGCAATGG CTGAAATGCC TTTTATGCAT AGCCTTAGTT TCCCTCTCTC TAGTACTGGG 
TTTTACCCCT CTTATGGCAT GCCTTATTCT CCCTCTCCAC TTGCAGCTGC TCCCATAGGA 
TTAGGATACT ATGGAAGGTA CCCTCCAACT CTTTATCCAC CACCCCCCTC TCCTTCTTTC 
ACCACTCCTT TGCCACCTCC TTCTTACATG CATGCTGGTC ATTTGCTTCT CAATCCTGCC 
AAGTACCACA AGAAGAAACA TAAGCTGCTT CGTCAGGAAG CCTTTCTTAC AACAAGTAGG 
ACACCCCTTC TCTCCATGAG CACCTACCCC AGTGTCCCTC CTGAGATGGC CTATGGTTGG 
ATGGTGGAGC ATAAACACCG GCACCGGCAC AAACACAGAG AACACCGTTC TTCTGAGCAA 
CCCCAGGTTT CCATGGACAC TTTAGCTACC AATAGCTCTT CCAGAACTGT CCTAGAATCG 
TTGAAGCGCT ATCGATTTGG TAAAGATGCT GTTGGGGAGC GATATAAACA CAAAGAGAAG 
CATCGTTGTC ACATGTCCTG TCCTCATCTG TCCCCATCAA AAAGCTTATT AAGCCGAGAG 
GAGCAATGGG TCCACCGAGA GCCTTCAGAA TCCACTTCAT TGGCCTTGGG GTTGCAGACA 
CCTTTACAGA TTGACTGTTC AGAAACGTCT CCAGGTTTGT CCCTGGGAGG TTTTACACCT 
AGCTCTGAGC CAGCCAGCAG TGATGAACAC ACAAACCTTT TCACAAGTGC AATAGGCAGC 
TGCAGAGTAT CAAACTCTAC TAATACAAGT GGCCGGAAGA AATTGACTGA CAGTCCAGGA 
CTCTTTACCG CACAGGACAC TTCATTAAAT CGGCCTCTTA GAAAGGAAAC TTTGCCTTCC 
AATGAAAGGG CACTGCAAAC TTTGACAGGC TCCCAGCCAG TCCTAGACAA GCCTTCCCAA 
AGGCCATCAG AGAGCACAAA CTGTAGCCCT ACTCGAAAGA GATCTTCATC AGAGAGCACT 
TCTTCAACAG TGATTGGAAT CCCTTCCCGA GGTACAAGAT TAGGAACATC TGTGGATGAC 
TCTGTGGATA GCCTGCTACA GCGTATGGCG CAACATGAAA GCCAAGGGAC CTTAGAGAAA 
AACATTGATG CTGTCATTGC CACAGTGTCC ATCCCACCTT CCTCAAGCCC AGGTCATACT 
CATGGTAGGG AACGAAGTCT GGGGAAATCA GACAGTCTGC TAGTACCCAC AGCACCTAGT 
GACTCCTGCA GCAACACCAC CACCACCATC TCACTCCTTT CTGAGAGGTT GCCAAGCAGC 
TGTTCCCCCC ATCATCTCAA AAGGAGTGTG GTAGAAGCCA TGCAACGCCA AGCCCGGAAA 
ATGTGCAATT ATAACAAAAT CTTGGCCACC AAGAAAAACC TAGACCATGT CAATAAAATC 
TTAAAAGCCA AAAAGCTTCA AAGACAGGCA AGGACAGGTA ACAATTTTGT GAAGCGCAGA 
CCAGGCCGAC CCCGGAAATA TCCCCTCCAG GCTGTGGTGT CAATGCAGGC ATTCCAGGCT 
GCCCAATTTG CCAGCCCAGA ATTAGATGAA GGGGAGGAAG GCAGAGCACT ACATCTTGGT 
CCTGATACAG TTACAGATGT CATTGAGGCT GTTGTTCAGA GTGTGAACCT GAACCCAGAA 
CTTAGAAAAG GATGGAAAAG GAAAAGTTGG CTTCTGGAGG AAGAGGCAAG GAAAAGACAG 
AAGATACTTC CAGAGGAAGA ACAGGAAAAT AATAAAAGCT TTCCTGAGAC AACAATTGAA 
TCTCCTAGTC CTCCAGAAGC CCCACCAAAG CCACCTGAAC CTGAAAATAC CCTGCAGCCT 
TTGCCTTCTC TTATCCCTCG GGAAAAGAAG GCCCCACGTC CCCCAAAGAA GAAATATCAG 
AAAGCAGGGC TCTATTCTGA TGTATACAAA ACTACAGATC CAAAGAGCAG ACTGATACAA 
TTAAAGAAAG AAAAACTGGA GTATACTCCT GGAGAACATG AATATGGATT ATTCCCAGCC 
CCTATTCATG TTGGAAAGTA TTTGAGACAA AAGAGAATTG ACTTTCAGCT TCCTTATGAC 
ATCCTCTGGC AATGGAAACA CAATCAGCTA TATAAAAAGC CAGATGTCCC ACTATACAAA 
AAAATCCGTT CAAATGTCTA CGTTGATGTC AAACCTCTTT CTGGCTATGA GGCTACCACC 
TGCAACTGTA AAAAACCAGA TGATGGTGAT AGGAAGGGTT GTATGGATGA CTGCCTCAAC 
AGAATGATCT TTGCTGAGTG TTCCCCCAAT ACCTGCCCCT GTGGAGAGCA GTGCTGCAAT 
CAGAGGATAC AAAGGCATGA GTGGGTACAG TGTCTTGAGC GGTTCCGAGC TGAGGAGAAG 
GGATGGGGGA TCCGGACCAA AGAACCTTTG AAAGCTGGCC AGTTCATTAT TGAATACTTG 
GGAGAAGTTG TCAGTGAGCA GGAGTTCAGA AACCGAATGA TTGAACAGTA CCATAATCAC 
AGTGACCATT ACTGCCTAAA TCTGGACAGT GGAATGGTGA TTGACAGCTA CCGAATGGGC 
AATGAGGCCC GGTTCATCAA CCACAGCTGT AACCCAAATT GTGAAATGCA GAAATGGTCT 
GTTAATGGAG TCTACCGGAT TGGGCTCTAT GCCCTGAAAG ATATGCCAGC TGGTACTGAA 
CTAACCTATG ACTACAATTT TCATTCTTTT AATGTTGAGA AACAGCAACT TTGTAAGTGT 
GGTTTTGACA AATGCCGGGG TATCATCGGA GGCAAGAGCC AACGCATGAA TGGACTCACC 
AGCAAAAGCA GCCAACCTGT GACCACACAC AGACGGCCAG GACGGTCAAA AGAGAAGAGA 
AAATCCAAGC ACAAGTTGAA GAAAAGGAGA GGCCATCTTT CTGAGGAGCC TAATGAAAAT 
GTCAGTACCC CAACCAGAAT GACCCCCCAA CTGCAAATGA AGCCAATGTC CAACCGTGAA 
AGGAACTTTG TGTTAAAACA TCATGTATTC CTGGTCCGGA ACTGGGAGAA AATCAGACAG 
AAGCAAGAGG AGGTAAAACA CACCAGTGAC AATATCCATT CAGCTTCCTT ATATACCCGC 
TGGAATGGTA TCTGCCGAGA TGATGGAAAC ATCAAATCTG ATGTCTTCAT GACCCAGTTC 
TCCGCCTTGC AGACAGCCCG CTCAGTTCGT ACAAGGAGAT TGGCAGCTGC AGAGGAGAAC 
ATTGAAGTGG CCCGGGCAGC CCGCCTGGCC CAGATATTCA AAGAGATATG TGACAGCATC 
ATCTCCTACA AAGATTCCTC CCGACAGGCA TTGGCAACTC CACTGTTGAA CCTTCCCCCA 
AAGAAAAAGA ATGCTGATTA TTATGAGAAG ATATCTGACC CCTTAGATCT CATTACCATT 
GAGAAGCAGA TCCTGACAGG GTATTACAAG ACAGTGGAGG CGTTTGATGC TGACATGCTC 
AAGGTCTTCC GGAATGCTGA GAAATACTAT GGACGCAAGT CCCCAATTGG GAGAGATGTA 
TGTCGACTAC GCAAAGCATA CTACAATGCA CGGCATGAGG CATCAGCTCA AATTGATGAG 
ATTGTGGGGG AAACAGCAAG TGAAGCAGAC AGCAGCGAAA CCTCAGTCTG TGAGAAGGAA 
AATGGGCATG AGAAGGATGA TGATGTTATC CGCTGTATCT GCGGCCTCTA CAAGGATGAA 
GGCCTCATGA TTCAGTGTGA CAAGTGCATG GTGTGGCAGC ACTGTGATTG CATGGGAGTA 
AACTCAGATG TAGAACATTA CCTCTGTGAA CAATGTGATC CAAGGCCTGT GGACAGGGAA 
GTGCCCATGA TACCTCGGCC CCATTATGCC CAACCTGGCT GTGTCTACTT CATCTGTCTA 
CTCCGGGATG ACCTGCTGCT ACGTCAAGGT GACTGTGTGT ACCTGATGCG AGATAGTCGG 
AGAACCCCTG ATGGCCACCC AGTCCGTCAG TCTTATCGAT TGTTATCCCA TATCAACCGA 
GAGAAACTTG ATATCTTTCG AATTGAGAAG CTTTGGAAGA ATGAAAAAGA GGAGCGGTTT 
GCCTTTGGTC ATCATTATTT TCGTCCCCAT GAAACCCACC ACTCCCCATC ACGTCGATTT 
TATCACAATG AGCTGTTCCG AGTGCCACTG TATGAAATCA TTCCTCTGGA GGCTGTGGTG 
GGGACCTGTT GCGTATTAGA TCTGTACACA TATTGTAAAG GAAGGCCCAA AGGAGTGAAA 
GAGCAGGATG TATATATCTG TGACTACCGA CTTGATAAAT CAGCCCACCT GTTCTACAAG 
ATTCATCGGA ACCGCTATCC TGTCTGCACC AAACCCTATG CCTTTGACCA CTTCCCCAAG 
AAGCTCACTC CCAAGAGAGA TTTCTCACCT CATTATGTCC CAGACAACTA CAAGAGGAAT 
GGAGGAAGAT CATCCTGGAA GACAGAACGT TCAAAGCCAC CCATCAAAGA CCTAGGCCAG 
GAGGATGATG CCCTACCACT CATTGAAGAA GTACTGGCCA GTCAAGAGCA AGCAGCCAAT 
GAGATGCCCA CCCTAGAAGA AACTGATCGG GAGGTAGCAC CTGGTGAAAC AACTGAGGGT 
GAAAAGAAAG CTGATGAAAG CAGACAGGAG CCTCAGAGGT CCTGCACACC TGAAGAGAGG 
AGACACAGCC AACGGGAGCG ACTCAACCAG ATTCTGCTCA ACCTTCTTGA GAAGATCCCT 
GGGAAGAATG CCATCGATGT GACATACTTG CTGGAGGAAG GATCAGGAAG AAAGCTTCGA 
AGACGTACTC TGCTTATCCC AGAGAGCAGC TTCCGAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031822579.1
CDS	502..9483
Translation	MDHRNTAMLGVGTDSEGFSRKSPSAVSTGTLVSKREVELENSTKEEEDLWRRNRDRNIETGKNDGLTDAQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTSIKHPRKALKSGKMTDEKNELCPSKRTMSGIYISTLQNNMYVTHWRRGEKDPSKLYKKANDVAAVECRSEEGIHLHSQGENNPVSKKLSPLHSEMTDYVKSAPSTLVGNRDPDSKDRALLNGATSVTEKLAQLIATCPPSKSSKTKPKKLGAGITAGLVSKDLIRKPGIGPMAGIIHKDLIKKPTISPAVGLVTKDPGKKPIFSAAVGLVSKDSGKKLGTGTTAVFINKDLGKKPGTITTVGLVSRESGKKLGIGIVPGLVSKESGKKLGLGTVVGLVNKDSVKKFGPAIGLVAKDCGKKTITNSSVGLVNKDPAKKLVACPVAGLVNKDIVNLKAEALLPVQEPLKVSCNTNTSSHESQEHSESLKDSATSKAFEKTIIRQNKDSLLEKFSIRKEIITLDKDIFNEGTCIQQDSFLSSERGTYVETTKHEKQPPVYCTSPDFQMGGTSDASTAKSPFSAIGESNLPSPSPALSVTPLNRSPPGTSSQLATNPLHLGPSTELIEGIAESIGKTQFSSENSHMNLGHRSLVHSMNTDCKGNDKELNDSKASHTDNSRMGSSIGKKPSLASESSIHATITPSVVSFTSLFSSKPFLKIGAVTASEKHCQVAESLSTSLQSKPLKKRKGRKPRWTKVVARNTCRPSKGLELERSELFKNVSCSSLSNSNSEPAKFMKNIGPSSFVEHDFLKCRLPKLSKSSTAPSLALLTETDKQSHKSFASHKLSSSICMSGDLLPDIYKPKRGRPKTKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQIRSPVKMKPPVLSVAPFVATESPSKLESESDKHRSSSDFFERKDQLQGPDDSHKPSVCGMSDLEMEPDKKINKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSTLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKAVLNGILTSSPASLAVLEKTAHQVAGSALGQILPPLLPSAASSSEVLPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHIHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFLAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPTFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLAAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPAKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDTLATNSSSRTVLESLKRYRFGKDAVGERYKHKEKHRCHMSCPHLSPSKSLLSREEQWVHREPSESTSLALGLQTPLQIDCSETSPGLSLGGFTPSSEPASSDEHTNLFTSAIGSCRVSNSTNTSGRKKLTDSPGLFTAQDTSLNRPLRKETLPSNERALQTLTGSQPVLDKPSQRPSESTNCSPTRKRSSSESTSSTVIGIPSRGTRLGTSVDDSVDSLLQRMAQHESQGTLEKNIDAVIATVSIPPSSSPGHTHGRERSLGKSDSLLVPTAPSDSCSNTTTTISLLSERLPSSCSPHHLKRSVVEAMQRQARKMCNYNKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKYPLQAVVSMQAFQAAQFASPELDEGEEGRALHLGPDTVTDVIEAVVQSVNLNPELRKGWKRKSWLLEEEARKRQKILPEEEQENNKSFPETTIESPSPPEAPPKPPEPENTLQPLPSLIPREKKAPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPDDGDRKGCMDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCNPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFDKCRGIIGGKSQRMNGLTSKSSQPVTTHRRPGRSKEKRKSKHKLKKRRGHLSEEPNENVSTPTRMTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIRQKQEEVKHTSDNIHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENIEVARAARLAQIFKEICDSIISYKDSSRQALATPLLNLPPKKKNADYYEKISDPLDLITIEKQILTGYYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKAYYNARHEASAQIDEIVGETASEADSSETSVCEKENGHEKDDDVIRCICGLYKDEGLMIQCDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINREKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKTERSKPPIKDLGQEDDALPLIEEVLASQEQAANEMPTLEETDREVAPGETTEGEKKADESRQEPQRSCTPEERRHSQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLLIPESSFRK

Target ORF information:

RefSeq Version	XM_031966719.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031966719.1

ORF Insert Sequence:

ATGGACCATA GGAATACTGC TATGTTAGGA GTGGGTACCG ACTCCGAAGG ATTTTCAAGG 
AAGAGTCCTT CTGCTGTCAG CACTGGAACA CTGGTTAGTA AGAGAGAAGT GGAACTAGAG 
AATAGCACTA AGGAAGAAGA GGATCTTTGG AGGCGGAATA GGGACAGAAA CATTGAGACA 
GGGAAAAATG ATGGTTTGAC TGATGCACAG CAGTTTTCTG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAGTTGAA AATTGGCCTT CAGGCTAAGA GAACTAAAAA GCCTCCTAAA 
AACTTGGAGA ACTATGTATG TCGACCTGCT ATAAAAACAA GCATTAAGCA TCCAAGGAAA 
GCATTGAAAA GTGGGAAGAT GACAGATGAA AAAAATGAAC TTTGCCCTTC AAAACGAACT 
ATGTCGGGAA TATATATCTC CACATTGCAA AACAACATGT ATGTTACACA TTGGAGGAGG 
GGAGAAAAAG ACCCTTCAAA ACTTTACAAG AAAGCAAATG ATGTTGCAGC TGTTGAATGC 
CGCTCTGAAG AAGGCATCCA CCTTCATTCA CAGGGAGAAA ATAATCCTGT GTCAAAGAAG 
CTTTCTCCAC TACACTCTGA AATGACAGAT TATGTTAAAT CAGCACCATC TACTCTTGTT 
GGTAACCGTG ATCCTGACTC GAAGGACAGA GCTCTACTTA ATGGAGCAAC AAGTGTAACA 
GAAAAATTGG CACAGCTCAT TGCTACTTGT CCACCCTCCA AGTCTTCCAA GACAAAGCCA 
AAAAAGTTAG GGGCTGGTAT TACAGCAGGA TTGGTTAGCA AAGACTTGAT CAGAAAGCCA 
GGCATTGGTC CTATGGCAGG AATTATTCAC AAGGACTTGA TTAAGAAACC AACAATTAGC 
CCAGCAGTTG GGTTGGTAAC TAAAGACCCT GGGAAAAAGC CAATCTTTAG TGCTGCAGTA 
GGATTGGTCA GTAAAGACTC TGGGAAAAAA TTAGGAACTG GGACTACTGC GGTATTCATA 
AACAAGGACT TAGGAAAGAA ACCAGGGACT ATCACTACAG TGGGACTGGT GAGCAGAGAG 
TCAGGGAAGA AATTAGGAAT TGGTATCGTC CCAGGTTTGG TGAGTAAAGA ATCAGGAAAG 
AAGTTGGGAC TTGGTACTGT AGTTGGGCTT GTTAATAAAG ATTCAGTAAA GAAGTTTGGC 
CCTGCCATTG GACTGGTTGC AAAGGACTGT GGGAAGAAGA CTATAACAAA TTCATCAGTG 
GGATTGGTTA ATAAGGACCC TGCAAAGAAA TTGGTGGCTT GCCCTGTAGC AGGATTGGTC 
AACAAAGACA TTGTGAATTT AAAAGCTGAA GCCTTGCTCC CTGTTCAAGA ACCACTTAAG 
GTTTCTTGCA ATACAAATAC TAGTAGTCAT GAAAGTCAGG AGCATTCTGA ATCCCTGAAA 
GATAGTGCCA CCAGCAAAGC TTTTGAGAAG ACTATTATTC GTCAGAATAA AGATAGTTTA 
TTAGAAAAGT TCTCAATTCG AAAAGAAATC ATTACTTTGG ACAAAGACAT TTTTAATGAA 
GGAACTTGCA TACAGCAGGA CAGTTTCTTA TCCAGTGAAA GGGGGACCTA TGTTGAAACT 
ACAAAGCATG AGAAGCAGCC TCCAGTGTAT TGCACTTCAC CAGATTTTCA AATGGGAGGC 
ACTTCTGATG CATCAACAGC TAAGTCTCCC TTTAGTGCAA TAGGGGAGAG TAATCTCCCT 
TCCCCTTCAC CAGCCTTGTC TGTTACGCCC TTAAACAGAA GTCCCCCTGG AACTTCTTCT 
CAGTTGGCTA CTAATCCATT ACATTTAGGT CCTTCCACAG AACTCATAGA AGGAATTGCT 
GAATCTATTG GAAAAACCCA ATTCAGTTCT GAAAATTCCC ATATGAACCT TGGTCACAGG 
TCTCTGGTTC ACAGCATGAA CACTGATTGC AAAGGAAATG ATAAAGAGTT AAATGATTCA 
AAAGCTTCTC ACACAGATAA CTCAAGAATG GGCTCCTCCA TAGGAAAAAA GCCAAGTCTG 
GCTTCAGAAT CCAGTATTCA TGCTACAATT ACTCCCTCAG TTGTTAGTTT CACTAGTTTA 
TTTAGCAGTA AACCCTTTCT GAAGATTGGT GCAGTAACTG CATCAGAGAA ACACTGCCAA 
GTTGCTGAAA GCCTGAGCAC TAGTTTGCAG TCCAAACCAT TAAAAAAAAG AAAAGGAAGG 
AAACCACGAT GGACTAAAGT GGTAGCAAGA AACACATGCC GGCCTTCAAA AGGGCTAGAA 
TTAGAAAGGT CAGAGCTTTT TAAAAATGTT TCATGTAGCT CTTTGTCAAA TAGCAATTCC 
GAGCCAGCCA AGTTTATGAA AAACATTGGG CCATCTTCAT TTGTGGAGCA TGACTTCCTT 
AAATGCCGAT TGCCAAAGCT GAGTAAATCT AGCACAGCTC CATCTCTTGC TCTCTTAACT 
GAAACTGACA AGCAGTCTCA TAAGTCCTTT GCCAGCCACA AACTTTCCTC TAGTATATGC 
ATGTCTGGTG ACCTTTTACC TGACATTTAT AAACCTAAAA GAGGAAGACC TAAAACTAAG 
GAGATGCCCC AACTAGAAGG TCCACCCAAA AGGACCCTAA AAATCCCGGC CTCTAAAGTT 
TTTTCATTAC AGTCAAAAGA GGAGCAAGAA CCCCCTATTT TGCAGCCAGA AATTGAAATC 
CCTTCCTTCA AGCAGAGCCT ATCAGTATCA CCCTTTCCCA AAAAAAGAGG AAGGCCAAAA 
AGACAAATCA GATCACCAGT CAAGATGAAA CCACCTGTGC TATCAGTGGC ACCCTTTGTA 
GCCACTGAAA GTCCAAGTAA ACTAGAGTCT GAGAGTGATA AACATAGAAG TAGTAGTGAC 
TTCTTTGAAA GGAAAGATCA ACTTCAGGGT CCAGATGACA GTCATAAACC AAGTGTGTGT 
GGTATGAGTG ACCTTGAGAT GGAACCTGAT AAAAAAATTA ATAAGAGAAA CAATGGCCAA 
TTAATGAAAA CTATCATTCG CAAAATAAAT AAGATGAAGA CTTTGAAGAG AAAGAAACTG 
CTGAATCAGA TTCTCTCAAG TTCTGTTGAA TCAAGTAACA AAGGGAAAGT GCAATCCAAA 
CTCCACAATA CAGTGTCAAC ACTTGCTGCC ACTTTTGGCT CCAAACTGGG CCAACAGATA 
AATGTCAGCA AGAAAGGAAC CATTTATATA GGAAAGAGGC GAGGCCGGAA ACCAAAAGCT 
GTGCTGAACG GCATCTTAAC TAGTAGCCCA GCTAGTCTGG CTGTCCTAGA GAAAACAGCT 
CATCAGGTAG CTGGGTCAGC ATTAGGACAG ATTCTCCCCC CACTCTTGCC TTCAGCAGCA 
AGTAGTTCTG AGGTTCTTCC ATCCCCCATC TGTTCTCAGT CTTCTGGGAC TAGTGGGGGC 
CAGAGTCCTG TCAGCAGTGA TGCAGGATTT GTTGAGCCCA GTTCAGTGCC ATATTTACAT 
ATACACTCCA GACAGGGCAG TATGATTCAG ACACTTGCTA TGAAGAAGGC TTCAAAGGGA 
AGGAGACGGT TGTCTCCTCC TACTTTGTTG CCAAATTCTC CTTCTCACTT GAGTGAACTC 
ACTTCTCTGA AGGAAGCTAC TCCTTCCCCA ATTAGTGAAT CTCATAGTGA TGAGACCATT 
CCTAGTGATA GTGGAATTGG AACAGATAAT AATAGCACAT CAGACAGGGC AGAAAAGTTT 
TGTGGGCAGA AAAAGAGGAG GCATTCTTTT GAACATGTTT CTCTCATTCC CCCTGAAACC 
TCCACAGTCC TAAGTAGTCT CAAAGAGAAA CATAAACACA AATGTAAACG CAGGAGTCAT 
GATTACCTCA GTTATGACAA AATGAAAAGA CAGAAGCGAA AACGGAAAAA GAAGTATCCC 
CAGTTACGAA ACAGACAGGA TCCAGACTTT CTTGCTGAGC TGGAGGAATT AATAAGTCGT 
TTAAGTGAAA TTCGGATCAC TCATCGAAGT CATCATTTTA TTCCCCGTGA TCTCCTACCC 
ACTATTTTTC GAATTAACTT TAACAGTTTC TATACGCACC CCACTTTTCC CTTAGACCCT 
TTGCACTACA TTAGAAAACC TGACTTAAAG AAAAAAAGAG GGAGGCCTCC CAAGATGAGG 
GAAGCAATGG CTGAAATGCC TTTTATGCAT AGCCTTAGTT TCCCTCTCTC TAGTACTGGG 
TTTTACCCCT CTTATGGCAT GCCTTATTCT CCCTCTCCAC TTGCAGCTGC TCCCATAGGA 
TTAGGATACT ATGGAAGGTA CCCTCCAACT CTTTATCCAC CACCCCCCTC TCCTTCTTTC 
ACCACTCCTT TGCCACCTCC TTCTTACATG CATGCTGGTC ATTTGCTTCT CAATCCTGCC 
AAGTACCACA AGAAGAAACA TAAGCTGCTT CGTCAGGAAG CCTTTCTTAC AACAAGTAGG 
ACACCCCTTC TCTCCATGAG CACCTACCCC AGTGTCCCTC CTGAGATGGC CTATGGTTGG 
ATGGTGGAGC ATAAACACCG GCACCGGCAC AAACACAGAG AACACCGTTC TTCTGAGCAA 
CCCCAGGTTT CCATGGACAC TTTAGCTACC AATAGCTCTT CCAGAACTGT CCTAGAATCG 
TTGAAGCGCT ATCGATTTGG TAAAGATGCT GTTGGGGAGC GATATAAACA CAAAGAGAAG 
CATCGTTGTC ACATGTCCTG TCCTCATCTG TCCCCATCAA AAAGCTTATT AAGCCGAGAG 
GAGCAATGGG TCCACCGAGA GCCTTCAGAA TCCACTTCAT TGGCCTTGGG GTTGCAGACA 
CCTTTACAGA TTGACTGTTC AGAAACGTCT CCAGGTTTGT CCCTGGGAGG TTTTACACCT 
AGCTCTGAGC CAGCCAGCAG TGATGAACAC ACAAACCTTT TCACAAGTGC AATAGGCAGC 
TGCAGAGTAT CAAACTCTAC TAATACAAGT GGCCGGAAGA AATTGACTGA CAGTCCAGGA 
CTCTTTACCG CACAGGACAC TTCATTAAAT CGGCCTCTTA GAAAGGAAAC TTTGCCTTCC 
AATGAAAGGG CACTGCAAAC TTTGACAGGC TCCCAGCCAG TCCTAGACAA GCCTTCCCAA 
AGGCCATCAG AGAGCACAAA CTGTAGCCCT ACTCGAAAGA GATCTTCATC AGAGAGCACT 
TCTTCAACAG TGATTGGAAT CCCTTCCCGA GGTACAAGAT TAGGAACATC TGTGGATGAC 
TCTGTGGATA GCCTGCTACA GCGTATGGCG CAACATGAAA GCCAAGGGAC CTTAGAGAAA 
AACATTGATG CTGTCATTGC CACAGTGTCC ATCCCACCTT CCTCAAGCCC AGGTCATACT 
CATGGTAGGG AACGAAGTCT GGGGAAATCA GACAGTCTGC TAGTACCCAC AGCACCTAGT 
GACTCCTGCA GCAACACCAC CACCACCATC TCACTCCTTT CTGAGAGGTT GCCAAGCAGC 
TGTTCCCCCC ATCATCTCAA AAGGAGTGTG GTAGAAGCCA TGCAACGCCA AGCCCGGAAA 
ATGTGCAATT ATAACAAAAT CTTGGCCACC AAGAAAAACC TAGACCATGT CAATAAAATC 
TTAAAAGCCA AAAAGCTTCA AAGACAGGCA AGGACAGGTA ACAATTTTGT GAAGCGCAGA 
CCAGGCCGAC CCCGGAAATA TCCCCTCCAG GCTGTGGTGT CAATGCAGGC ATTCCAGGCT 
GCCCAATTTG CCAGCCCAGA ATTAGATGAA GGGGAGGAAG GCAGAGCACT ACATCTTGGT 
CCTGATACAG TTACAGATGT CATTGAGGCT GTTGTTCAGA GTGTGAACCT GAACCCAGAA 
CTTAGAAAAG GATGGAAAAG GAAAAGTTGG CTTCTGGAGG AAGAGGCAAG GAAAAGACAG 
AAGATACTTC CAGAGGAAGA ACAGGAAAAT AATAAAAGCT TTCCTGAGAC AACAATTGAA 
TCTCCTAGTC CTCCAGAAGC CCCACCAAAG CCACCTGAAC CTGAAAATAC CCTGCAGCCT 
TTGCCTTCTC TTATCCCTCG GGAAAAGAAG GCCCCACGTC CCCCAAAGAA GAAATATCAG 
AAAGCAGGGC TCTATTCTGA TGTATACAAA ACTACAGATC CAAAGAGCAG ACTGATACAA 
TTAAAGAAAG AAAAACTGGA GTATACTCCT GGAGAACATG AATATGGATT ATTCCCAGCC 
CCTATTCATG TTGGAAAGTA TTTGAGACAA AAGAGAATTG ACTTTCAGCT TCCTTATGAC 
ATCCTCTGGC AATGGAAACA CAATCAGCTA TATAAAAAGC CAGATGTCCC ACTATACAAA 
AAAATCCGTT CAAATGTCTA CGTTGATGTC AAACCTCTTT CTGGCTATGA GGCTACCACC 
TGCAACTGTA AAAAACCAGA TGATGGTGAT AGGAAGGGTT GTATGGATGA CTGCCTCAAC 
AGAATGATCT TTGCTGAGTG TTCCCCCAAT ACCTGCCCCT GTGGAGAGCA GTGCTGCAAT 
CAGAGGATAC AAAGGCATGA GTGGGTACAG TGTCTTGAGC GGTTCCGAGC TGAGGAGAAG 
GGATGGGGGA TCCGGACCAA AGAACCTTTG AAAGCTGGCC AGTTCATTAT TGAATACTTG 
GGAGAAGTTG TCAGTGAGCA GGAGTTCAGA AACCGAATGA TTGAACAGTA CCATAATCAC 
AGTGACCATT ACTGCCTAAA TCTGGACAGT GGAATGGTGA TTGACAGCTA CCGAATGGGC 
AATGAGGCCC GGTTCATCAA CCACAGCTGT AACCCAAATT GTGAAATGCA GAAATGGTCT 
GTTAATGGAG TCTACCGGAT TGGGCTCTAT GCCCTGAAAG ATATGCCAGC TGGTACTGAA 
CTAACCTATG ACTACAATTT TCATTCTTTT AATGTTGAGA AACAGCAACT TTGTAAGTGT 
GGTTTTGACA AATGCCGGGG TATCATCGGA GGCAAGAGCC AACGCATGAA TGGACTCACC 
AGCAAAAGCA GCCAACCTGT GACCACACAC AGACGGCCAG GACGGTCAAA AGAGAAGAGA 
AAATCCAAGC ACAAGTTGAA GAAAAGGAGA GGCCATCTTT CTGAGGAGCC TAATGAAAAT 
GTCAGTACCC CAACCAGAAT GACCCCCCAA CTGCAAATGA AGCCAATGTC CAACCGTGAA 
AGGAACTTTG TGTTAAAACA TCATGTATTC CTGGTCCGGA ACTGGGAGAA AATCAGACAG 
AAGCAAGAGG AGGTAAAACA CACCAGTGAC AATATCCATT CAGCTTCCTT ATATACCCGC 
TGGAATGGTA TCTGCCGAGA TGATGGAAAC ATCAAATCTG ATGTCTTCAT GACCCAGTTC 
TCCGCCTTGC AGACAGCCCG CTCAGTTCGT ACAAGGAGAT TGGCAGCTGC AGAGGAGAAC 
ATTGAAGTGG CCCGGGCAGC CCGCCTGGCC CAGATATTCA AAGAGATATG TGACAGCATC 
ATCTCCTACA AAGATTCCTC CCGACAGGCA TTGGCAACTC CACTGTTGAA CCTTCCCCCA 
AAGAAAAAGA ATGCTGATTA TTATGAGAAG ATATCTGACC CCTTAGATCT CATTACCATT 
GAGAAGCAGA TCCTGACAGG GTATTACAAG ACAGTGGAGG CGTTTGATGC TGACATGCTC 
AAGGTCTTCC GGAATGCTGA GAAATACTAT GGACGCAAGT CCCCAATTGG GAGAGATGTA 
TGTCGACTAC GCAAAGCATA CTACAATGCA CGGCATGAGG CATCAGCTCA AATTGATGAG 
ATTGTGGGGG AAACAGCAAG TGAAGCAGAC AGCAGCGAAA CCTCAGTCTG TGAGAAGGAA 
AATGGGCATG AGAAGGATGA TGATGTTATC CGCTGTATCT GCGGCCTCTA CAAGGATGAA 
GGCCTCATGA TTCAGTGTGA CAAGTGCATG GTGTGGCAGC ACTGTGATTG CATGGGAGTA 
AACTCAGATG TAGAACATTA CCTCTGTGAA CAATGTGATC CAAGGCCTGT GGACAGGGAA 
GTGCCCATGA TACCTCGGCC CCATTATGCC CAACCTGGCT GTGTCTACTT CATCTGTCTA 
CTCCGGGATG ACCTGCTGCT ACGTCAAGGT GACTGTGTGT ACCTGATGCG AGATAGTCGG 
AGAACCCCTG ATGGCCACCC AGTCCGTCAG TCTTATCGAT TGTTATCCCA TATCAACCGA 
GAGAAACTTG ATATCTTTCG AATTGAGAAG CTTTGGAAGA ATGAAAAAGA GGAGCGGTTT 
GCCTTTGGTC ATCATTATTT TCGTCCCCAT GAAACCCACC ACTCCCCATC ACGTCGATTT 
TATCACAATG AGCTGTTCCG AGTGCCACTG TATGAAATCA TTCCTCTGGA GGCTGTGGTG 
GGGACCTGTT GCGTATTAGA TCTGTACACA TATTGTAAAG GAAGGCCCAA AGGAGTGAAA 
GAGCAGGATG TATATATCTG TGACTACCGA CTTGATAAAT CAGCCCACCT GTTCTACAAG 
ATTCATCGGA ACCGCTATCC TGTCTGCACC AAACCCTATG CCTTTGACCA CTTCCCCAAG 
AAGCTCACTC CCAAGAGAGA TTTCTCACCT CATTATGTCC CAGACAACTA CAAGAGGAAT 
GGAGGAAGAT CATCCTGGAA GACAGAACGT TCAAAGCCAC CCATCAAAGA CCTAGGCCAG 
GAGGATGATG CCCTACCACT CATTGAAGAA GTACTGGCCA GTCAAGAGCA AGCAGCCAAT 
GAGATGCCCA CCCTAGAAGA AACTGATCGG GAGGTAGCAC CTGGTGAAAC AACTGAGGGT 
GAAAAGAAAG CTGATGAAAG CAGACAGGAG CCTCAGAGGT CCTGCACACC TGAAGAGAGG 
AGACACAGCC AACGGGAGCG ACTCAACCAG ATTCTGCTCA ACCTTCTTGA GAAGATCCCT 
GGGAAGAATG CCATCGATGT GACATACTTG CTGGAGGAAG GATCAGGAAG AAAGCTTCGA 
AGACGTACTC TGCTTATCCC AGAGAGCAGC TTCCGAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASH1L ( XM_031966721.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031822581.1 Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X3, mRNA.

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CloneID	OSa154248
Clone ID Related Accession (Same CDS sequence)	XM_023502250.1 , XM_031966722.1 , XM_031966719.1 , XM_031966721.1 , XM_031966720.1
Accession Version	XM_031966721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	histone-lysine N-methyltransferase ASH1L isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATA GGAATACTGC TATGTTAGGA GTGGGTACCG ACTCCGAAGG ATTTTCAAGG 
AAGAGTCCTT CTGCTGTCAG CACTGGAACA CTGGTTAGTA AGAGAGAAGT GGAACTAGAG 
AATAGCACTA AGGAAGAAGA GGATCTTTGG AGGCGGAATA GGGACAGAAA CATTGAGACA 
GGGAAAAATG ATGGTTTGAC TGATGCACAG CAGTTTTCTG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAGTTGAA AATTGGCCTT CAGGCTAAGA GAACTAAAAA GCCTCCTAAA 
AACTTGGAGA ACTATGTATG TCGACCTGCT ATAAAAACAA GCATTAAGCA TCCAAGGAAA 
GCATTGAAAA GTGGGAAGAT GACAGATGAA AAAAATGAAC TTTGCCCTTC AAAACGAACT 
ATGTCGGGAA TATATATCTC CACATTGCAA AACAACATGT ATGTTACACA TTGGAGGAGG 
GGAGAAAAAG ACCCTTCAAA ACTTTACAAG AAAGCAAATG ATGTTGCAGC TGTTGAATGC 
CGCTCTGAAG AAGGCATCCA CCTTCATTCA CAGGGAGAAA ATAATCCTGT GTCAAAGAAG 
CTTTCTCCAC TACACTCTGA AATGACAGAT TATGTTAAAT CAGCACCATC TACTCTTGTT 
GGTAACCGTG ATCCTGACTC GAAGGACAGA GCTCTACTTA ATGGAGCAAC AAGTGTAACA 
GAAAAATTGG CACAGCTCAT TGCTACTTGT CCACCCTCCA AGTCTTCCAA GACAAAGCCA 
AAAAAGTTAG GGGCTGGTAT TACAGCAGGA TTGGTTAGCA AAGACTTGAT CAGAAAGCCA 
GGCATTGGTC CTATGGCAGG AATTATTCAC AAGGACTTGA TTAAGAAACC AACAATTAGC 
CCAGCAGTTG GGTTGGTAAC TAAAGACCCT GGGAAAAAGC CAATCTTTAG TGCTGCAGTA 
GGATTGGTCA GTAAAGACTC TGGGAAAAAA TTAGGAACTG GGACTACTGC GGTATTCATA 
AACAAGGACT TAGGAAAGAA ACCAGGGACT ATCACTACAG TGGGACTGGT GAGCAGAGAG 
TCAGGGAAGA AATTAGGAAT TGGTATCGTC CCAGGTTTGG TGAGTAAAGA ATCAGGAAAG 
AAGTTGGGAC TTGGTACTGT AGTTGGGCTT GTTAATAAAG ATTCAGTAAA GAAGTTTGGC 
CCTGCCATTG GACTGGTTGC AAAGGACTGT GGGAAGAAGA CTATAACAAA TTCATCAGTG 
GGATTGGTTA ATAAGGACCC TGCAAAGAAA TTGGTGGCTT GCCCTGTAGC AGGATTGGTC 
AACAAAGACA TTGTGAATTT AAAAGCTGAA GCCTTGCTCC CTGTTCAAGA ACCACTTAAG 
GTTTCTTGCA ATACAAATAC TAGTAGTCAT GAAAGTCAGG AGCATTCTGA ATCCCTGAAA 
GATAGTGCCA CCAGCAAAGC TTTTGAGAAG ACTATTATTC GTCAGAATAA AGATAGTTTA 
TTAGAAAAGT TCTCAATTCG AAAAGAAATC ATTACTTTGG ACAAAGACAT TTTTAATGAA 
GGAACTTGCA TACAGCAGGA CAGTTTCTTA TCCAGTGAAA GGGGGACCTA TGTTGAAACT 
ACAAAGCATG AGAAGCAGCC TCCAGTGTAT TGCACTTCAC CAGATTTTCA AATGGGAGGC 
ACTTCTGATG CATCAACAGC TAAGTCTCCC TTTAGTGCAA TAGGGGAGAG TAATCTCCCT 
TCCCCTTCAC CAGCCTTGTC TGTTACGCCC TTAAACAGAA GTCCCCCTGG AACTTCTTCT 
CAGTTGGCTA CTAATCCATT ACATTTAGGT CCTTCCACAG AACTCATAGA AGGAATTGCT 
GAATCTATTG GAAAAACCCA ATTCAGTTCT GAAAATTCCC ATATGAACCT TGGTCACAGG 
TCTCTGGTTC ACAGCATGAA CACTGATTGC AAAGGAAATG ATAAAGAGTT AAATGATTCA 
AAAGCTTCTC ACACAGATAA CTCAAGAATG GGCTCCTCCA TAGGAAAAAA GCCAAGTCTG 
GCTTCAGAAT CCAGTATTCA TGCTACAATT ACTCCCTCAG TTGTTAGTTT CACTAGTTTA 
TTTAGCAGTA AACCCTTTCT GAAGATTGGT GCAGTAACTG CATCAGAGAA ACACTGCCAA 
GTTGCTGAAA GCCTGAGCAC TAGTTTGCAG TCCAAACCAT TAAAAAAAAG AAAAGGAAGG 
AAACCACGAT GGACTAAAGT GGTAGCAAGA AACACATGCC GGCCTTCAAA AGGGCTAGAA 
TTAGAAAGGT CAGAGCTTTT TAAAAATGTT TCATGTAGCT CTTTGTCAAA TAGCAATTCC 
GAGCCAGCCA AGTTTATGAA AAACATTGGG CCATCTTCAT TTGTGGAGCA TGACTTCCTT 
AAATGCCGAT TGCCAAAGCT GAGTAAATCT AGCACAGCTC CATCTCTTGC TCTCTTAACT 
GAAACTGACA AGCAGTCTCA TAAGTCCTTT GCCAGCCACA AACTTTCCTC TAGTATATGC 
ATGTCTGGTG ACCTTTTACC TGACATTTAT AAACCTAAAA GAGGAAGACC TAAAACTAAG 
GAGATGCCCC AACTAGAAGG TCCACCCAAA AGGACCCTAA AAATCCCGGC CTCTAAAGTT 
TTTTCATTAC AGTCAAAAGA GGAGCAAGAA CCCCCTATTT TGCAGCCAGA AATTGAAATC 
CCTTCCTTCA AGCAGAGCCT ATCAGTATCA CCCTTTCCCA AAAAAAGAGG AAGGCCAAAA 
AGACAAATCA GATCACCAGT CAAGATGAAA CCACCTGTGC TATCAGTGGC ACCCTTTGTA 
GCCACTGAAA GTCCAAGTAA ACTAGAGTCT GAGAGTGATA AACATAGAAG TAGTAGTGAC 
TTCTTTGAAA GGAAAGATCA ACTTCAGGGT CCAGATGACA GTCATAAACC AAGTGTGTGT 
GGTATGAGTG ACCTTGAGAT GGAACCTGAT AAAAAAATTA ATAAGAGAAA CAATGGCCAA 
TTAATGAAAA CTATCATTCG CAAAATAAAT AAGATGAAGA CTTTGAAGAG AAAGAAACTG 
CTGAATCAGA TTCTCTCAAG TTCTGTTGAA TCAAGTAACA AAGGGAAAGT GCAATCCAAA 
CTCCACAATA CAGTGTCAAC ACTTGCTGCC ACTTTTGGCT CCAAACTGGG CCAACAGATA 
AATGTCAGCA AGAAAGGAAC CATTTATATA GGAAAGAGGC GAGGCCGGAA ACCAAAAGCT 
GTGCTGAACG GCATCTTAAC TAGTAGCCCA GCTAGTCTGG CTGTCCTAGA GAAAACAGCT 
CATCAGGTAG CTGGGTCAGC ATTAGGACAG ATTCTCCCCC CACTCTTGCC TTCAGCAGCA 
AGTAGTTCTG AGGTTCTTCC ATCCCCCATC TGTTCTCAGT CTTCTGGGAC TAGTGGGGGC 
CAGAGTCCTG TCAGCAGTGA TGCAGGATTT GTTGAGCCCA GTTCAGTGCC ATATTTACAT 
ATACACTCCA GACAGGGCAG TATGATTCAG ACACTTGCTA TGAAGAAGGC TTCAAAGGGA 
AGGAGACGGT TGTCTCCTCC TACTTTGTTG CCAAATTCTC CTTCTCACTT GAGTGAACTC 
ACTTCTCTGA AGGAAGCTAC TCCTTCCCCA ATTAGTGAAT CTCATAGTGA TGAGACCATT 
CCTAGTGATA GTGGAATTGG AACAGATAAT AATAGCACAT CAGACAGGGC AGAAAAGTTT 
TGTGGGCAGA AAAAGAGGAG GCATTCTTTT GAACATGTTT CTCTCATTCC CCCTGAAACC 
TCCACAGTCC TAAGTAGTCT CAAAGAGAAA CATAAACACA AATGTAAACG CAGGAGTCAT 
GATTACCTCA GTTATGACAA AATGAAAAGA CAGAAGCGAA AACGGAAAAA GAAGTATCCC 
CAGTTACGAA ACAGACAGGA TCCAGACTTT CTTGCTGAGC TGGAGGAATT AATAAGTCGT 
TTAAGTGAAA TTCGGATCAC TCATCGAAGT CATCATTTTA TTCCCCGTGA TCTCCTACCC 
ACTATTTTTC GAATTAACTT TAACAGTTTC TATACGCACC CCACTTTTCC CTTAGACCCT 
TTGCACTACA TTAGAAAACC TGACTTAAAG AAAAAAAGAG GGAGGCCTCC CAAGATGAGG 
GAAGCAATGG CTGAAATGCC TTTTATGCAT AGCCTTAGTT TCCCTCTCTC TAGTACTGGG 
TTTTACCCCT CTTATGGCAT GCCTTATTCT CCCTCTCCAC TTGCAGCTGC TCCCATAGGA 
TTAGGATACT ATGGAAGGTA CCCTCCAACT CTTTATCCAC CACCCCCCTC TCCTTCTTTC 
ACCACTCCTT TGCCACCTCC TTCTTACATG CATGCTGGTC ATTTGCTTCT CAATCCTGCC 
AAGTACCACA AGAAGAAACA TAAGCTGCTT CGTCAGGAAG CCTTTCTTAC AACAAGTAGG 
ACACCCCTTC TCTCCATGAG CACCTACCCC AGTGTCCCTC CTGAGATGGC CTATGGTTGG 
ATGGTGGAGC ATAAACACCG GCACCGGCAC AAACACAGAG AACACCGTTC TTCTGAGCAA 
CCCCAGGTTT CCATGGACAC TTTAGCTACC AATAGCTCTT CCAGAACTGT CCTAGAATCG 
TTGAAGCGCT ATCGATTTGG TAAAGATGCT GTTGGGGAGC GATATAAACA CAAAGAGAAG 
CATCGTTGTC ACATGTCCTG TCCTCATCTG TCCCCATCAA AAAGCTTATT AAGCCGAGAG 
GAGCAATGGG TCCACCGAGA GCCTTCAGAA TCCACTTCAT TGGCCTTGGG GTTGCAGACA 
CCTTTACAGA TTGACTGTTC AGAAACGTCT CCAGGTTTGT CCCTGGGAGG TTTTACACCT 
AGCTCTGAGC CAGCCAGCAG TGATGAACAC ACAAACCTTT TCACAAGTGC AATAGGCAGC 
TGCAGAGTAT CAAACTCTAC TAATACAAGT GGCCGGAAGA AATTGACTGA CAGTCCAGGA 
CTCTTTACCG CACAGGACAC TTCATTAAAT CGGCCTCTTA GAAAGGAAAC TTTGCCTTCC 
AATGAAAGGG CACTGCAAAC TTTGACAGGC TCCCAGCCAG TCCTAGACAA GCCTTCCCAA 
AGGCCATCAG AGAGCACAAA CTGTAGCCCT ACTCGAAAGA GATCTTCATC AGAGAGCACT 
TCTTCAACAG TGATTGGAAT CCCTTCCCGA GGTACAAGAT TAGGAACATC TGTGGATGAC 
TCTGTGGATA GCCTGCTACA GCGTATGGCG CAACATGAAA GCCAAGGGAC CTTAGAGAAA 
AACATTGATG CTGTCATTGC CACAGTGTCC ATCCCACCTT CCTCAAGCCC AGGTCATACT 
CATGGTAGGG AACGAAGTCT GGGGAAATCA GACAGTCTGC TAGTACCCAC AGCACCTAGT 
GACTCCTGCA GCAACACCAC CACCACCATC TCACTCCTTT CTGAGAGGTT GCCAAGCAGC 
TGTTCCCCCC ATCATCTCAA AAGGAGTGTG GTAGAAGCCA TGCAACGCCA AGCCCGGAAA 
ATGTGCAATT ATAACAAAAT CTTGGCCACC AAGAAAAACC TAGACCATGT CAATAAAATC 
TTAAAAGCCA AAAAGCTTCA AAGACAGGCA AGGACAGGTA ACAATTTTGT GAAGCGCAGA 
CCAGGCCGAC CCCGGAAATA TCCCCTCCAG GCTGTGGTGT CAATGCAGGC ATTCCAGGCT 
GCCCAATTTG CCAGCCCAGA ATTAGATGAA GGGGAGGAAG GCAGAGCACT ACATCTTGGT 
CCTGATACAG TTACAGATGT CATTGAGGCT GTTGTTCAGA GTGTGAACCT GAACCCAGAA 
CTTAGAAAAG GATGGAAAAG GAAAAGTTGG CTTCTGGAGG AAGAGGCAAG GAAAAGACAG 
AAGATACTTC CAGAGGAAGA ACAGGAAAAT AATAAAAGCT TTCCTGAGAC AACAATTGAA 
TCTCCTAGTC CTCCAGAAGC CCCACCAAAG CCACCTGAAC CTGAAAATAC CCTGCAGCCT 
TTGCCTTCTC TTATCCCTCG GGAAAAGAAG GCCCCACGTC CCCCAAAGAA GAAATATCAG 
AAAGCAGGGC TCTATTCTGA TGTATACAAA ACTACAGATC CAAAGAGCAG ACTGATACAA 
TTAAAGAAAG AAAAACTGGA GTATACTCCT GGAGAACATG AATATGGATT ATTCCCAGCC 
CCTATTCATG TTGGAAAGTA TTTGAGACAA AAGAGAATTG ACTTTCAGCT TCCTTATGAC 
ATCCTCTGGC AATGGAAACA CAATCAGCTA TATAAAAAGC CAGATGTCCC ACTATACAAA 
AAAATCCGTT CAAATGTCTA CGTTGATGTC AAACCTCTTT CTGGCTATGA GGCTACCACC 
TGCAACTGTA AAAAACCAGA TGATGGTGAT AGGAAGGGTT GTATGGATGA CTGCCTCAAC 
AGAATGATCT TTGCTGAGTG TTCCCCCAAT ACCTGCCCCT GTGGAGAGCA GTGCTGCAAT 
CAGAGGATAC AAAGGCATGA GTGGGTACAG TGTCTTGAGC GGTTCCGAGC TGAGGAGAAG 
GGATGGGGGA TCCGGACCAA AGAACCTTTG AAAGCTGGCC AGTTCATTAT TGAATACTTG 
GGAGAAGTTG TCAGTGAGCA GGAGTTCAGA AACCGAATGA TTGAACAGTA CCATAATCAC 
AGTGACCATT ACTGCCTAAA TCTGGACAGT GGAATGGTGA TTGACAGCTA CCGAATGGGC 
AATGAGGCCC GGTTCATCAA CCACAGCTGT AACCCAAATT GTGAAATGCA GAAATGGTCT 
GTTAATGGAG TCTACCGGAT TGGGCTCTAT GCCCTGAAAG ATATGCCAGC TGGTACTGAA 
CTAACCTATG ACTACAATTT TCATTCTTTT AATGTTGAGA AACAGCAACT TTGTAAGTGT 
GGTTTTGACA AATGCCGGGG TATCATCGGA GGCAAGAGCC AACGCATGAA TGGACTCACC 
AGCAAAAGCA GCCAACCTGT GACCACACAC AGACGGCCAG GACGGTCAAA AGAGAAGAGA 
AAATCCAAGC ACAAGTTGAA GAAAAGGAGA GGCCATCTTT CTGAGGAGCC TAATGAAAAT 
GTCAGTACCC CAACCAGAAT GACCCCCCAA CTGCAAATGA AGCCAATGTC CAACCGTGAA 
AGGAACTTTG TGTTAAAACA TCATGTATTC CTGGTCCGGA ACTGGGAGAA AATCAGACAG 
AAGCAAGAGG AGGTAAAACA CACCAGTGAC AATATCCATT CAGCTTCCTT ATATACCCGC 
TGGAATGGTA TCTGCCGAGA TGATGGAAAC ATCAAATCTG ATGTCTTCAT GACCCAGTTC 
TCCGCCTTGC AGACAGCCCG CTCAGTTCGT ACAAGGAGAT TGGCAGCTGC AGAGGAGAAC 
ATTGAAGTGG CCCGGGCAGC CCGCCTGGCC CAGATATTCA AAGAGATATG TGACAGCATC 
ATCTCCTACA AAGATTCCTC CCGACAGGCA TTGGCAACTC CACTGTTGAA CCTTCCCCCA 
AAGAAAAAGA ATGCTGATTA TTATGAGAAG ATATCTGACC CCTTAGATCT CATTACCATT 
GAGAAGCAGA TCCTGACAGG GTATTACAAG ACAGTGGAGG CGTTTGATGC TGACATGCTC 
AAGGTCTTCC GGAATGCTGA GAAATACTAT GGACGCAAGT CCCCAATTGG GAGAGATGTA 
TGTCGACTAC GCAAAGCATA CTACAATGCA CGGCATGAGG CATCAGCTCA AATTGATGAG 
ATTGTGGGGG AAACAGCAAG TGAAGCAGAC AGCAGCGAAA CCTCAGTCTG TGAGAAGGAA 
AATGGGCATG AGAAGGATGA TGATGTTATC CGCTGTATCT GCGGCCTCTA CAAGGATGAA 
GGCCTCATGA TTCAGTGTGA CAAGTGCATG GTGTGGCAGC ACTGTGATTG CATGGGAGTA 
AACTCAGATG TAGAACATTA CCTCTGTGAA CAATGTGATC CAAGGCCTGT GGACAGGGAA 
GTGCCCATGA TACCTCGGCC CCATTATGCC CAACCTGGCT GTGTCTACTT CATCTGTCTA 
CTCCGGGATG ACCTGCTGCT ACGTCAAGGT GACTGTGTGT ACCTGATGCG AGATAGTCGG 
AGAACCCCTG ATGGCCACCC AGTCCGTCAG TCTTATCGAT TGTTATCCCA TATCAACCGA 
GAGAAACTTG ATATCTTTCG AATTGAGAAG CTTTGGAAGA ATGAAAAAGA GGAGCGGTTT 
GCCTTTGGTC ATCATTATTT TCGTCCCCAT GAAACCCACC ACTCCCCATC ACGTCGATTT 
TATCACAATG AGCTGTTCCG AGTGCCACTG TATGAAATCA TTCCTCTGGA GGCTGTGGTG 
GGGACCTGTT GCGTATTAGA TCTGTACACA TATTGTAAAG GAAGGCCCAA AGGAGTGAAA 
GAGCAGGATG TATATATCTG TGACTACCGA CTTGATAAAT CAGCCCACCT GTTCTACAAG 
ATTCATCGGA ACCGCTATCC TGTCTGCACC AAACCCTATG CCTTTGACCA CTTCCCCAAG 
AAGCTCACTC CCAAGAGAGA TTTCTCACCT CATTATGTCC CAGACAACTA CAAGAGGAAT 
GGAGGAAGAT CATCCTGGAA GACAGAACGT TCAAAGCCAC CCATCAAAGA CCTAGGCCAG 
GAGGATGATG CCCTACCACT CATTGAAGAA GTACTGGCCA GTCAAGAGCA AGCAGCCAAT 
GAGATGCCCA CCCTAGAAGA AACTGATCGG GAGGTAGCAC CTGGTGAAAC AACTGAGGGT 
GAAAAGAAAG CTGATGAAAG CAGACAGGAG CCTCAGAGGT CCTGCACACC TGAAGAGAGG 
AGACACAGCC AACGGGAGCG ACTCAACCAG ATTCTGCTCA ACCTTCTTGA GAAGATCCCT 
GGGAAGAATG CCATCGATGT GACATACTTG CTGGAGGAAG GATCAGGAAG AAAGCTTCGA 
AGACGTACTC TGCTTATCCC AGAGAGCAGC TTCCGAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031822581.1
CDS	163..9144
Translation	MDHRNTAMLGVGTDSEGFSRKSPSAVSTGTLVSKREVELENSTKEEEDLWRRNRDRNIETGKNDGLTDAQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTSIKHPRKALKSGKMTDEKNELCPSKRTMSGIYISTLQNNMYVTHWRRGEKDPSKLYKKANDVAAVECRSEEGIHLHSQGENNPVSKKLSPLHSEMTDYVKSAPSTLVGNRDPDSKDRALLNGATSVTEKLAQLIATCPPSKSSKTKPKKLGAGITAGLVSKDLIRKPGIGPMAGIIHKDLIKKPTISPAVGLVTKDPGKKPIFSAAVGLVSKDSGKKLGTGTTAVFINKDLGKKPGTITTVGLVSRESGKKLGIGIVPGLVSKESGKKLGLGTVVGLVNKDSVKKFGPAIGLVAKDCGKKTITNSSVGLVNKDPAKKLVACPVAGLVNKDIVNLKAEALLPVQEPLKVSCNTNTSSHESQEHSESLKDSATSKAFEKTIIRQNKDSLLEKFSIRKEIITLDKDIFNEGTCIQQDSFLSSERGTYVETTKHEKQPPVYCTSPDFQMGGTSDASTAKSPFSAIGESNLPSPSPALSVTPLNRSPPGTSSQLATNPLHLGPSTELIEGIAESIGKTQFSSENSHMNLGHRSLVHSMNTDCKGNDKELNDSKASHTDNSRMGSSIGKKPSLASESSIHATITPSVVSFTSLFSSKPFLKIGAVTASEKHCQVAESLSTSLQSKPLKKRKGRKPRWTKVVARNTCRPSKGLELERSELFKNVSCSSLSNSNSEPAKFMKNIGPSSFVEHDFLKCRLPKLSKSSTAPSLALLTETDKQSHKSFASHKLSSSICMSGDLLPDIYKPKRGRPKTKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQIRSPVKMKPPVLSVAPFVATESPSKLESESDKHRSSSDFFERKDQLQGPDDSHKPSVCGMSDLEMEPDKKINKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSTLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKAVLNGILTSSPASLAVLEKTAHQVAGSALGQILPPLLPSAASSSEVLPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHIHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFLAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPTFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLAAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPAKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDTLATNSSSRTVLESLKRYRFGKDAVGERYKHKEKHRCHMSCPHLSPSKSLLSREEQWVHREPSESTSLALGLQTPLQIDCSETSPGLSLGGFTPSSEPASSDEHTNLFTSAIGSCRVSNSTNTSGRKKLTDSPGLFTAQDTSLNRPLRKETLPSNERALQTLTGSQPVLDKPSQRPSESTNCSPTRKRSSSESTSSTVIGIPSRGTRLGTSVDDSVDSLLQRMAQHESQGTLEKNIDAVIATVSIPPSSSPGHTHGRERSLGKSDSLLVPTAPSDSCSNTTTTISLLSERLPSSCSPHHLKRSVVEAMQRQARKMCNYNKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKYPLQAVVSMQAFQAAQFASPELDEGEEGRALHLGPDTVTDVIEAVVQSVNLNPELRKGWKRKSWLLEEEARKRQKILPEEEQENNKSFPETTIESPSPPEAPPKPPEPENTLQPLPSLIPREKKAPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPDDGDRKGCMDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCNPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFDKCRGIIGGKSQRMNGLTSKSSQPVTTHRRPGRSKEKRKSKHKLKKRRGHLSEEPNENVSTPTRMTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIRQKQEEVKHTSDNIHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENIEVARAARLAQIFKEICDSIISYKDSSRQALATPLLNLPPKKKNADYYEKISDPLDLITIEKQILTGYYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKAYYNARHEASAQIDEIVGETASEADSSETSVCEKENGHEKDDDVIRCICGLYKDEGLMIQCDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINREKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKTERSKPPIKDLGQEDDALPLIEEVLASQEQAANEMPTLEETDREVAPGETTEGEKKADESRQEPQRSCTPEERRHSQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLLIPESSFRK

Target ORF information:

RefSeq Version	XM_031966721.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031966721.1

ORF Insert Sequence:

ATGGACCATA GGAATACTGC TATGTTAGGA GTGGGTACCG ACTCCGAAGG ATTTTCAAGG 
AAGAGTCCTT CTGCTGTCAG CACTGGAACA CTGGTTAGTA AGAGAGAAGT GGAACTAGAG 
AATAGCACTA AGGAAGAAGA GGATCTTTGG AGGCGGAATA GGGACAGAAA CATTGAGACA 
GGGAAAAATG ATGGTTTGAC TGATGCACAG CAGTTTTCTG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAGTTGAA AATTGGCCTT CAGGCTAAGA GAACTAAAAA GCCTCCTAAA 
AACTTGGAGA ACTATGTATG TCGACCTGCT ATAAAAACAA GCATTAAGCA TCCAAGGAAA 
GCATTGAAAA GTGGGAAGAT GACAGATGAA AAAAATGAAC TTTGCCCTTC AAAACGAACT 
ATGTCGGGAA TATATATCTC CACATTGCAA AACAACATGT ATGTTACACA TTGGAGGAGG 
GGAGAAAAAG ACCCTTCAAA ACTTTACAAG AAAGCAAATG ATGTTGCAGC TGTTGAATGC 
CGCTCTGAAG AAGGCATCCA CCTTCATTCA CAGGGAGAAA ATAATCCTGT GTCAAAGAAG 
CTTTCTCCAC TACACTCTGA AATGACAGAT TATGTTAAAT CAGCACCATC TACTCTTGTT 
GGTAACCGTG ATCCTGACTC GAAGGACAGA GCTCTACTTA ATGGAGCAAC AAGTGTAACA 
GAAAAATTGG CACAGCTCAT TGCTACTTGT CCACCCTCCA AGTCTTCCAA GACAAAGCCA 
AAAAAGTTAG GGGCTGGTAT TACAGCAGGA TTGGTTAGCA AAGACTTGAT CAGAAAGCCA 
GGCATTGGTC CTATGGCAGG AATTATTCAC AAGGACTTGA TTAAGAAACC AACAATTAGC 
CCAGCAGTTG GGTTGGTAAC TAAAGACCCT GGGAAAAAGC CAATCTTTAG TGCTGCAGTA 
GGATTGGTCA GTAAAGACTC TGGGAAAAAA TTAGGAACTG GGACTACTGC GGTATTCATA 
AACAAGGACT TAGGAAAGAA ACCAGGGACT ATCACTACAG TGGGACTGGT GAGCAGAGAG 
TCAGGGAAGA AATTAGGAAT TGGTATCGTC CCAGGTTTGG TGAGTAAAGA ATCAGGAAAG 
AAGTTGGGAC TTGGTACTGT AGTTGGGCTT GTTAATAAAG ATTCAGTAAA GAAGTTTGGC 
CCTGCCATTG GACTGGTTGC AAAGGACTGT GGGAAGAAGA CTATAACAAA TTCATCAGTG 
GGATTGGTTA ATAAGGACCC TGCAAAGAAA TTGGTGGCTT GCCCTGTAGC AGGATTGGTC 
AACAAAGACA TTGTGAATTT AAAAGCTGAA GCCTTGCTCC CTGTTCAAGA ACCACTTAAG 
GTTTCTTGCA ATACAAATAC TAGTAGTCAT GAAAGTCAGG AGCATTCTGA ATCCCTGAAA 
GATAGTGCCA CCAGCAAAGC TTTTGAGAAG ACTATTATTC GTCAGAATAA AGATAGTTTA 
TTAGAAAAGT TCTCAATTCG AAAAGAAATC ATTACTTTGG ACAAAGACAT TTTTAATGAA 
GGAACTTGCA TACAGCAGGA CAGTTTCTTA TCCAGTGAAA GGGGGACCTA TGTTGAAACT 
ACAAAGCATG AGAAGCAGCC TCCAGTGTAT TGCACTTCAC CAGATTTTCA AATGGGAGGC 
ACTTCTGATG CATCAACAGC TAAGTCTCCC TTTAGTGCAA TAGGGGAGAG TAATCTCCCT 
TCCCCTTCAC CAGCCTTGTC TGTTACGCCC TTAAACAGAA GTCCCCCTGG AACTTCTTCT 
CAGTTGGCTA CTAATCCATT ACATTTAGGT CCTTCCACAG AACTCATAGA AGGAATTGCT 
GAATCTATTG GAAAAACCCA ATTCAGTTCT GAAAATTCCC ATATGAACCT TGGTCACAGG 
TCTCTGGTTC ACAGCATGAA CACTGATTGC AAAGGAAATG ATAAAGAGTT AAATGATTCA 
AAAGCTTCTC ACACAGATAA CTCAAGAATG GGCTCCTCCA TAGGAAAAAA GCCAAGTCTG 
GCTTCAGAAT CCAGTATTCA TGCTACAATT ACTCCCTCAG TTGTTAGTTT CACTAGTTTA 
TTTAGCAGTA AACCCTTTCT GAAGATTGGT GCAGTAACTG CATCAGAGAA ACACTGCCAA 
GTTGCTGAAA GCCTGAGCAC TAGTTTGCAG TCCAAACCAT TAAAAAAAAG AAAAGGAAGG 
AAACCACGAT GGACTAAAGT GGTAGCAAGA AACACATGCC GGCCTTCAAA AGGGCTAGAA 
TTAGAAAGGT CAGAGCTTTT TAAAAATGTT TCATGTAGCT CTTTGTCAAA TAGCAATTCC 
GAGCCAGCCA AGTTTATGAA AAACATTGGG CCATCTTCAT TTGTGGAGCA TGACTTCCTT 
AAATGCCGAT TGCCAAAGCT GAGTAAATCT AGCACAGCTC CATCTCTTGC TCTCTTAACT 
GAAACTGACA AGCAGTCTCA TAAGTCCTTT GCCAGCCACA AACTTTCCTC TAGTATATGC 
ATGTCTGGTG ACCTTTTACC TGACATTTAT AAACCTAAAA GAGGAAGACC TAAAACTAAG 
GAGATGCCCC AACTAGAAGG TCCACCCAAA AGGACCCTAA AAATCCCGGC CTCTAAAGTT 
TTTTCATTAC AGTCAAAAGA GGAGCAAGAA CCCCCTATTT TGCAGCCAGA AATTGAAATC 
CCTTCCTTCA AGCAGAGCCT ATCAGTATCA CCCTTTCCCA AAAAAAGAGG AAGGCCAAAA 
AGACAAATCA GATCACCAGT CAAGATGAAA CCACCTGTGC TATCAGTGGC ACCCTTTGTA 
GCCACTGAAA GTCCAAGTAA ACTAGAGTCT GAGAGTGATA AACATAGAAG TAGTAGTGAC 
TTCTTTGAAA GGAAAGATCA ACTTCAGGGT CCAGATGACA GTCATAAACC AAGTGTGTGT 
GGTATGAGTG ACCTTGAGAT GGAACCTGAT AAAAAAATTA ATAAGAGAAA CAATGGCCAA 
TTAATGAAAA CTATCATTCG CAAAATAAAT AAGATGAAGA CTTTGAAGAG AAAGAAACTG 
CTGAATCAGA TTCTCTCAAG TTCTGTTGAA TCAAGTAACA AAGGGAAAGT GCAATCCAAA 
CTCCACAATA CAGTGTCAAC ACTTGCTGCC ACTTTTGGCT CCAAACTGGG CCAACAGATA 
AATGTCAGCA AGAAAGGAAC CATTTATATA GGAAAGAGGC GAGGCCGGAA ACCAAAAGCT 
GTGCTGAACG GCATCTTAAC TAGTAGCCCA GCTAGTCTGG CTGTCCTAGA GAAAACAGCT 
CATCAGGTAG CTGGGTCAGC ATTAGGACAG ATTCTCCCCC CACTCTTGCC TTCAGCAGCA 
AGTAGTTCTG AGGTTCTTCC ATCCCCCATC TGTTCTCAGT CTTCTGGGAC TAGTGGGGGC 
CAGAGTCCTG TCAGCAGTGA TGCAGGATTT GTTGAGCCCA GTTCAGTGCC ATATTTACAT 
ATACACTCCA GACAGGGCAG TATGATTCAG ACACTTGCTA TGAAGAAGGC TTCAAAGGGA 
AGGAGACGGT TGTCTCCTCC TACTTTGTTG CCAAATTCTC CTTCTCACTT GAGTGAACTC 
ACTTCTCTGA AGGAAGCTAC TCCTTCCCCA ATTAGTGAAT CTCATAGTGA TGAGACCATT 
CCTAGTGATA GTGGAATTGG AACAGATAAT AATAGCACAT CAGACAGGGC AGAAAAGTTT 
TGTGGGCAGA AAAAGAGGAG GCATTCTTTT GAACATGTTT CTCTCATTCC CCCTGAAACC 
TCCACAGTCC TAAGTAGTCT CAAAGAGAAA CATAAACACA AATGTAAACG CAGGAGTCAT 
GATTACCTCA GTTATGACAA AATGAAAAGA CAGAAGCGAA AACGGAAAAA GAAGTATCCC 
CAGTTACGAA ACAGACAGGA TCCAGACTTT CTTGCTGAGC TGGAGGAATT AATAAGTCGT 
TTAAGTGAAA TTCGGATCAC TCATCGAAGT CATCATTTTA TTCCCCGTGA TCTCCTACCC 
ACTATTTTTC GAATTAACTT TAACAGTTTC TATACGCACC CCACTTTTCC CTTAGACCCT 
TTGCACTACA TTAGAAAACC TGACTTAAAG AAAAAAAGAG GGAGGCCTCC CAAGATGAGG 
GAAGCAATGG CTGAAATGCC TTTTATGCAT AGCCTTAGTT TCCCTCTCTC TAGTACTGGG 
TTTTACCCCT CTTATGGCAT GCCTTATTCT CCCTCTCCAC TTGCAGCTGC TCCCATAGGA 
TTAGGATACT ATGGAAGGTA CCCTCCAACT CTTTATCCAC CACCCCCCTC TCCTTCTTTC 
ACCACTCCTT TGCCACCTCC TTCTTACATG CATGCTGGTC ATTTGCTTCT CAATCCTGCC 
AAGTACCACA AGAAGAAACA TAAGCTGCTT CGTCAGGAAG CCTTTCTTAC AACAAGTAGG 
ACACCCCTTC TCTCCATGAG CACCTACCCC AGTGTCCCTC CTGAGATGGC CTATGGTTGG 
ATGGTGGAGC ATAAACACCG GCACCGGCAC AAACACAGAG AACACCGTTC TTCTGAGCAA 
CCCCAGGTTT CCATGGACAC TTTAGCTACC AATAGCTCTT CCAGAACTGT CCTAGAATCG 
TTGAAGCGCT ATCGATTTGG TAAAGATGCT GTTGGGGAGC GATATAAACA CAAAGAGAAG 
CATCGTTGTC ACATGTCCTG TCCTCATCTG TCCCCATCAA AAAGCTTATT AAGCCGAGAG 
GAGCAATGGG TCCACCGAGA GCCTTCAGAA TCCACTTCAT TGGCCTTGGG GTTGCAGACA 
CCTTTACAGA TTGACTGTTC AGAAACGTCT CCAGGTTTGT CCCTGGGAGG TTTTACACCT 
AGCTCTGAGC CAGCCAGCAG TGATGAACAC ACAAACCTTT TCACAAGTGC AATAGGCAGC 
TGCAGAGTAT CAAACTCTAC TAATACAAGT GGCCGGAAGA AATTGACTGA CAGTCCAGGA 
CTCTTTACCG CACAGGACAC TTCATTAAAT CGGCCTCTTA GAAAGGAAAC TTTGCCTTCC 
AATGAAAGGG CACTGCAAAC TTTGACAGGC TCCCAGCCAG TCCTAGACAA GCCTTCCCAA 
AGGCCATCAG AGAGCACAAA CTGTAGCCCT ACTCGAAAGA GATCTTCATC AGAGAGCACT 
TCTTCAACAG TGATTGGAAT CCCTTCCCGA GGTACAAGAT TAGGAACATC TGTGGATGAC 
TCTGTGGATA GCCTGCTACA GCGTATGGCG CAACATGAAA GCCAAGGGAC CTTAGAGAAA 
AACATTGATG CTGTCATTGC CACAGTGTCC ATCCCACCTT CCTCAAGCCC AGGTCATACT 
CATGGTAGGG AACGAAGTCT GGGGAAATCA GACAGTCTGC TAGTACCCAC AGCACCTAGT 
GACTCCTGCA GCAACACCAC CACCACCATC TCACTCCTTT CTGAGAGGTT GCCAAGCAGC 
TGTTCCCCCC ATCATCTCAA AAGGAGTGTG GTAGAAGCCA TGCAACGCCA AGCCCGGAAA 
ATGTGCAATT ATAACAAAAT CTTGGCCACC AAGAAAAACC TAGACCATGT CAATAAAATC 
TTAAAAGCCA AAAAGCTTCA AAGACAGGCA AGGACAGGTA ACAATTTTGT GAAGCGCAGA 
CCAGGCCGAC CCCGGAAATA TCCCCTCCAG GCTGTGGTGT CAATGCAGGC ATTCCAGGCT 
GCCCAATTTG CCAGCCCAGA ATTAGATGAA GGGGAGGAAG GCAGAGCACT ACATCTTGGT 
CCTGATACAG TTACAGATGT CATTGAGGCT GTTGTTCAGA GTGTGAACCT GAACCCAGAA 
CTTAGAAAAG GATGGAAAAG GAAAAGTTGG CTTCTGGAGG AAGAGGCAAG GAAAAGACAG 
AAGATACTTC CAGAGGAAGA ACAGGAAAAT AATAAAAGCT TTCCTGAGAC AACAATTGAA 
TCTCCTAGTC CTCCAGAAGC CCCACCAAAG CCACCTGAAC CTGAAAATAC CCTGCAGCCT 
TTGCCTTCTC TTATCCCTCG GGAAAAGAAG GCCCCACGTC CCCCAAAGAA GAAATATCAG 
AAAGCAGGGC TCTATTCTGA TGTATACAAA ACTACAGATC CAAAGAGCAG ACTGATACAA 
TTAAAGAAAG AAAAACTGGA GTATACTCCT GGAGAACATG AATATGGATT ATTCCCAGCC 
CCTATTCATG TTGGAAAGTA TTTGAGACAA AAGAGAATTG ACTTTCAGCT TCCTTATGAC 
ATCCTCTGGC AATGGAAACA CAATCAGCTA TATAAAAAGC CAGATGTCCC ACTATACAAA 
AAAATCCGTT CAAATGTCTA CGTTGATGTC AAACCTCTTT CTGGCTATGA GGCTACCACC 
TGCAACTGTA AAAAACCAGA TGATGGTGAT AGGAAGGGTT GTATGGATGA CTGCCTCAAC 
AGAATGATCT TTGCTGAGTG TTCCCCCAAT ACCTGCCCCT GTGGAGAGCA GTGCTGCAAT 
CAGAGGATAC AAAGGCATGA GTGGGTACAG TGTCTTGAGC GGTTCCGAGC TGAGGAGAAG 
GGATGGGGGA TCCGGACCAA AGAACCTTTG AAAGCTGGCC AGTTCATTAT TGAATACTTG 
GGAGAAGTTG TCAGTGAGCA GGAGTTCAGA AACCGAATGA TTGAACAGTA CCATAATCAC 
AGTGACCATT ACTGCCTAAA TCTGGACAGT GGAATGGTGA TTGACAGCTA CCGAATGGGC 
AATGAGGCCC GGTTCATCAA CCACAGCTGT AACCCAAATT GTGAAATGCA GAAATGGTCT 
GTTAATGGAG TCTACCGGAT TGGGCTCTAT GCCCTGAAAG ATATGCCAGC TGGTACTGAA 
CTAACCTATG ACTACAATTT TCATTCTTTT AATGTTGAGA AACAGCAACT TTGTAAGTGT 
GGTTTTGACA AATGCCGGGG TATCATCGGA GGCAAGAGCC AACGCATGAA TGGACTCACC 
AGCAAAAGCA GCCAACCTGT GACCACACAC AGACGGCCAG GACGGTCAAA AGAGAAGAGA 
AAATCCAAGC ACAAGTTGAA GAAAAGGAGA GGCCATCTTT CTGAGGAGCC TAATGAAAAT 
GTCAGTACCC CAACCAGAAT GACCCCCCAA CTGCAAATGA AGCCAATGTC CAACCGTGAA 
AGGAACTTTG TGTTAAAACA TCATGTATTC CTGGTCCGGA ACTGGGAGAA AATCAGACAG 
AAGCAAGAGG AGGTAAAACA CACCAGTGAC AATATCCATT CAGCTTCCTT ATATACCCGC 
TGGAATGGTA TCTGCCGAGA TGATGGAAAC ATCAAATCTG ATGTCTTCAT GACCCAGTTC 
TCCGCCTTGC AGACAGCCCG CTCAGTTCGT ACAAGGAGAT TGGCAGCTGC AGAGGAGAAC 
ATTGAAGTGG CCCGGGCAGC CCGCCTGGCC CAGATATTCA AAGAGATATG TGACAGCATC 
ATCTCCTACA AAGATTCCTC CCGACAGGCA TTGGCAACTC CACTGTTGAA CCTTCCCCCA 
AAGAAAAAGA ATGCTGATTA TTATGAGAAG ATATCTGACC CCTTAGATCT CATTACCATT 
GAGAAGCAGA TCCTGACAGG GTATTACAAG ACAGTGGAGG CGTTTGATGC TGACATGCTC 
AAGGTCTTCC GGAATGCTGA GAAATACTAT GGACGCAAGT CCCCAATTGG GAGAGATGTA 
TGTCGACTAC GCAAAGCATA CTACAATGCA CGGCATGAGG CATCAGCTCA AATTGATGAG 
ATTGTGGGGG AAACAGCAAG TGAAGCAGAC AGCAGCGAAA CCTCAGTCTG TGAGAAGGAA 
AATGGGCATG AGAAGGATGA TGATGTTATC CGCTGTATCT GCGGCCTCTA CAAGGATGAA 
GGCCTCATGA TTCAGTGTGA CAAGTGCATG GTGTGGCAGC ACTGTGATTG CATGGGAGTA 
AACTCAGATG TAGAACATTA CCTCTGTGAA CAATGTGATC CAAGGCCTGT GGACAGGGAA 
GTGCCCATGA TACCTCGGCC CCATTATGCC CAACCTGGCT GTGTCTACTT CATCTGTCTA 
CTCCGGGATG ACCTGCTGCT ACGTCAAGGT GACTGTGTGT ACCTGATGCG AGATAGTCGG 
AGAACCCCTG ATGGCCACCC AGTCCGTCAG TCTTATCGAT TGTTATCCCA TATCAACCGA 
GAGAAACTTG ATATCTTTCG AATTGAGAAG CTTTGGAAGA ATGAAAAAGA GGAGCGGTTT 
GCCTTTGGTC ATCATTATTT TCGTCCCCAT GAAACCCACC ACTCCCCATC ACGTCGATTT 
TATCACAATG AGCTGTTCCG AGTGCCACTG TATGAAATCA TTCCTCTGGA GGCTGTGGTG 
GGGACCTGTT GCGTATTAGA TCTGTACACA TATTGTAAAG GAAGGCCCAA AGGAGTGAAA 
GAGCAGGATG TATATATCTG TGACTACCGA CTTGATAAAT CAGCCCACCT GTTCTACAAG 
ATTCATCGGA ACCGCTATCC TGTCTGCACC AAACCCTATG CCTTTGACCA CTTCCCCAAG 
AAGCTCACTC CCAAGAGAGA TTTCTCACCT CATTATGTCC CAGACAACTA CAAGAGGAAT 
GGAGGAAGAT CATCCTGGAA GACAGAACGT TCAAAGCCAC CCATCAAAGA CCTAGGCCAG 
GAGGATGATG CCCTACCACT CATTGAAGAA GTACTGGCCA GTCAAGAGCA AGCAGCCAAT 
GAGATGCCCA CCCTAGAAGA AACTGATCGG GAGGTAGCAC CTGGTGAAAC AACTGAGGGT 
GAAAAGAAAG CTGATGAAAG CAGACAGGAG CCTCAGAGGT CCTGCACACC TGAAGAGAGG 
AGACACAGCC AACGGGAGCG ACTCAACCAG ATTCTGCTCA ACCTTCTTGA GAAGATCCCT 
GGGAAGAATG CCATCGATGT GACATACTTG CTGGAGGAAG GATCAGGAAG AAAGCTTCGA 
AGACGTACTC TGCTTATCCC AGAGAGCAGC TTCCGAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASH1L ( XM_031966720.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031822580.1 Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X2, mRNA.

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CloneID	OSa154248
Clone ID Related Accession (Same CDS sequence)	XM_023502250.1 , XM_031966722.1 , XM_031966719.1 , XM_031966721.1 , XM_031966720.1
Accession Version	XM_031966720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	histone-lysine N-methyltransferase ASH1L isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATA GGAATACTGC TATGTTAGGA GTGGGTACCG ACTCCGAAGG ATTTTCAAGG 
AAGAGTCCTT CTGCTGTCAG CACTGGAACA CTGGTTAGTA AGAGAGAAGT GGAACTAGAG 
AATAGCACTA AGGAAGAAGA GGATCTTTGG AGGCGGAATA GGGACAGAAA CATTGAGACA 
GGGAAAAATG ATGGTTTGAC TGATGCACAG CAGTTTTCTG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAGTTGAA AATTGGCCTT CAGGCTAAGA GAACTAAAAA GCCTCCTAAA 
AACTTGGAGA ACTATGTATG TCGACCTGCT ATAAAAACAA GCATTAAGCA TCCAAGGAAA 
GCATTGAAAA GTGGGAAGAT GACAGATGAA AAAAATGAAC TTTGCCCTTC AAAACGAACT 
ATGTCGGGAA TATATATCTC CACATTGCAA AACAACATGT ATGTTACACA TTGGAGGAGG 
GGAGAAAAAG ACCCTTCAAA ACTTTACAAG AAAGCAAATG ATGTTGCAGC TGTTGAATGC 
CGCTCTGAAG AAGGCATCCA CCTTCATTCA CAGGGAGAAA ATAATCCTGT GTCAAAGAAG 
CTTTCTCCAC TACACTCTGA AATGACAGAT TATGTTAAAT CAGCACCATC TACTCTTGTT 
GGTAACCGTG ATCCTGACTC GAAGGACAGA GCTCTACTTA ATGGAGCAAC AAGTGTAACA 
GAAAAATTGG CACAGCTCAT TGCTACTTGT CCACCCTCCA AGTCTTCCAA GACAAAGCCA 
AAAAAGTTAG GGGCTGGTAT TACAGCAGGA TTGGTTAGCA AAGACTTGAT CAGAAAGCCA 
GGCATTGGTC CTATGGCAGG AATTATTCAC AAGGACTTGA TTAAGAAACC AACAATTAGC 
CCAGCAGTTG GGTTGGTAAC TAAAGACCCT GGGAAAAAGC CAATCTTTAG TGCTGCAGTA 
GGATTGGTCA GTAAAGACTC TGGGAAAAAA TTAGGAACTG GGACTACTGC GGTATTCATA 
AACAAGGACT TAGGAAAGAA ACCAGGGACT ATCACTACAG TGGGACTGGT GAGCAGAGAG 
TCAGGGAAGA AATTAGGAAT TGGTATCGTC CCAGGTTTGG TGAGTAAAGA ATCAGGAAAG 
AAGTTGGGAC TTGGTACTGT AGTTGGGCTT GTTAATAAAG ATTCAGTAAA GAAGTTTGGC 
CCTGCCATTG GACTGGTTGC AAAGGACTGT GGGAAGAAGA CTATAACAAA TTCATCAGTG 
GGATTGGTTA ATAAGGACCC TGCAAAGAAA TTGGTGGCTT GCCCTGTAGC AGGATTGGTC 
AACAAAGACA TTGTGAATTT AAAAGCTGAA GCCTTGCTCC CTGTTCAAGA ACCACTTAAG 
GTTTCTTGCA ATACAAATAC TAGTAGTCAT GAAAGTCAGG AGCATTCTGA ATCCCTGAAA 
GATAGTGCCA CCAGCAAAGC TTTTGAGAAG ACTATTATTC GTCAGAATAA AGATAGTTTA 
TTAGAAAAGT TCTCAATTCG AAAAGAAATC ATTACTTTGG ACAAAGACAT TTTTAATGAA 
GGAACTTGCA TACAGCAGGA CAGTTTCTTA TCCAGTGAAA GGGGGACCTA TGTTGAAACT 
ACAAAGCATG AGAAGCAGCC TCCAGTGTAT TGCACTTCAC CAGATTTTCA AATGGGAGGC 
ACTTCTGATG CATCAACAGC TAAGTCTCCC TTTAGTGCAA TAGGGGAGAG TAATCTCCCT 
TCCCCTTCAC CAGCCTTGTC TGTTACGCCC TTAAACAGAA GTCCCCCTGG AACTTCTTCT 
CAGTTGGCTA CTAATCCATT ACATTTAGGT CCTTCCACAG AACTCATAGA AGGAATTGCT 
GAATCTATTG GAAAAACCCA ATTCAGTTCT GAAAATTCCC ATATGAACCT TGGTCACAGG 
TCTCTGGTTC ACAGCATGAA CACTGATTGC AAAGGAAATG ATAAAGAGTT AAATGATTCA 
AAAGCTTCTC ACACAGATAA CTCAAGAATG GGCTCCTCCA TAGGAAAAAA GCCAAGTCTG 
GCTTCAGAAT CCAGTATTCA TGCTACAATT ACTCCCTCAG TTGTTAGTTT CACTAGTTTA 
TTTAGCAGTA AACCCTTTCT GAAGATTGGT GCAGTAACTG CATCAGAGAA ACACTGCCAA 
GTTGCTGAAA GCCTGAGCAC TAGTTTGCAG TCCAAACCAT TAAAAAAAAG AAAAGGAAGG 
AAACCACGAT GGACTAAAGT GGTAGCAAGA AACACATGCC GGCCTTCAAA AGGGCTAGAA 
TTAGAAAGGT CAGAGCTTTT TAAAAATGTT TCATGTAGCT CTTTGTCAAA TAGCAATTCC 
GAGCCAGCCA AGTTTATGAA AAACATTGGG CCATCTTCAT TTGTGGAGCA TGACTTCCTT 
AAATGCCGAT TGCCAAAGCT GAGTAAATCT AGCACAGCTC CATCTCTTGC TCTCTTAACT 
GAAACTGACA AGCAGTCTCA TAAGTCCTTT GCCAGCCACA AACTTTCCTC TAGTATATGC 
ATGTCTGGTG ACCTTTTACC TGACATTTAT AAACCTAAAA GAGGAAGACC TAAAACTAAG 
GAGATGCCCC AACTAGAAGG TCCACCCAAA AGGACCCTAA AAATCCCGGC CTCTAAAGTT 
TTTTCATTAC AGTCAAAAGA GGAGCAAGAA CCCCCTATTT TGCAGCCAGA AATTGAAATC 
CCTTCCTTCA AGCAGAGCCT ATCAGTATCA CCCTTTCCCA AAAAAAGAGG AAGGCCAAAA 
AGACAAATCA GATCACCAGT CAAGATGAAA CCACCTGTGC TATCAGTGGC ACCCTTTGTA 
GCCACTGAAA GTCCAAGTAA ACTAGAGTCT GAGAGTGATA AACATAGAAG TAGTAGTGAC 
TTCTTTGAAA GGAAAGATCA ACTTCAGGGT CCAGATGACA GTCATAAACC AAGTGTGTGT 
GGTATGAGTG ACCTTGAGAT GGAACCTGAT AAAAAAATTA ATAAGAGAAA CAATGGCCAA 
TTAATGAAAA CTATCATTCG CAAAATAAAT AAGATGAAGA CTTTGAAGAG AAAGAAACTG 
CTGAATCAGA TTCTCTCAAG TTCTGTTGAA TCAAGTAACA AAGGGAAAGT GCAATCCAAA 
CTCCACAATA CAGTGTCAAC ACTTGCTGCC ACTTTTGGCT CCAAACTGGG CCAACAGATA 
AATGTCAGCA AGAAAGGAAC CATTTATATA GGAAAGAGGC GAGGCCGGAA ACCAAAAGCT 
GTGCTGAACG GCATCTTAAC TAGTAGCCCA GCTAGTCTGG CTGTCCTAGA GAAAACAGCT 
CATCAGGTAG CTGGGTCAGC ATTAGGACAG ATTCTCCCCC CACTCTTGCC TTCAGCAGCA 
AGTAGTTCTG AGGTTCTTCC ATCCCCCATC TGTTCTCAGT CTTCTGGGAC TAGTGGGGGC 
CAGAGTCCTG TCAGCAGTGA TGCAGGATTT GTTGAGCCCA GTTCAGTGCC ATATTTACAT 
ATACACTCCA GACAGGGCAG TATGATTCAG ACACTTGCTA TGAAGAAGGC TTCAAAGGGA 
AGGAGACGGT TGTCTCCTCC TACTTTGTTG CCAAATTCTC CTTCTCACTT GAGTGAACTC 
ACTTCTCTGA AGGAAGCTAC TCCTTCCCCA ATTAGTGAAT CTCATAGTGA TGAGACCATT 
CCTAGTGATA GTGGAATTGG AACAGATAAT AATAGCACAT CAGACAGGGC AGAAAAGTTT 
TGTGGGCAGA AAAAGAGGAG GCATTCTTTT GAACATGTTT CTCTCATTCC CCCTGAAACC 
TCCACAGTCC TAAGTAGTCT CAAAGAGAAA CATAAACACA AATGTAAACG CAGGAGTCAT 
GATTACCTCA GTTATGACAA AATGAAAAGA CAGAAGCGAA AACGGAAAAA GAAGTATCCC 
CAGTTACGAA ACAGACAGGA TCCAGACTTT CTTGCTGAGC TGGAGGAATT AATAAGTCGT 
TTAAGTGAAA TTCGGATCAC TCATCGAAGT CATCATTTTA TTCCCCGTGA TCTCCTACCC 
ACTATTTTTC GAATTAACTT TAACAGTTTC TATACGCACC CCACTTTTCC CTTAGACCCT 
TTGCACTACA TTAGAAAACC TGACTTAAAG AAAAAAAGAG GGAGGCCTCC CAAGATGAGG 
GAAGCAATGG CTGAAATGCC TTTTATGCAT AGCCTTAGTT TCCCTCTCTC TAGTACTGGG 
TTTTACCCCT CTTATGGCAT GCCTTATTCT CCCTCTCCAC TTGCAGCTGC TCCCATAGGA 
TTAGGATACT ATGGAAGGTA CCCTCCAACT CTTTATCCAC CACCCCCCTC TCCTTCTTTC 
ACCACTCCTT TGCCACCTCC TTCTTACATG CATGCTGGTC ATTTGCTTCT CAATCCTGCC 
AAGTACCACA AGAAGAAACA TAAGCTGCTT CGTCAGGAAG CCTTTCTTAC AACAAGTAGG 
ACACCCCTTC TCTCCATGAG CACCTACCCC AGTGTCCCTC CTGAGATGGC CTATGGTTGG 
ATGGTGGAGC ATAAACACCG GCACCGGCAC AAACACAGAG AACACCGTTC TTCTGAGCAA 
CCCCAGGTTT CCATGGACAC TTTAGCTACC AATAGCTCTT CCAGAACTGT CCTAGAATCG 
TTGAAGCGCT ATCGATTTGG TAAAGATGCT GTTGGGGAGC GATATAAACA CAAAGAGAAG 
CATCGTTGTC ACATGTCCTG TCCTCATCTG TCCCCATCAA AAAGCTTATT AAGCCGAGAG 
GAGCAATGGG TCCACCGAGA GCCTTCAGAA TCCACTTCAT TGGCCTTGGG GTTGCAGACA 
CCTTTACAGA TTGACTGTTC AGAAACGTCT CCAGGTTTGT CCCTGGGAGG TTTTACACCT 
AGCTCTGAGC CAGCCAGCAG TGATGAACAC ACAAACCTTT TCACAAGTGC AATAGGCAGC 
TGCAGAGTAT CAAACTCTAC TAATACAAGT GGCCGGAAGA AATTGACTGA CAGTCCAGGA 
CTCTTTACCG CACAGGACAC TTCATTAAAT CGGCCTCTTA GAAAGGAAAC TTTGCCTTCC 
AATGAAAGGG CACTGCAAAC TTTGACAGGC TCCCAGCCAG TCCTAGACAA GCCTTCCCAA 
AGGCCATCAG AGAGCACAAA CTGTAGCCCT ACTCGAAAGA GATCTTCATC AGAGAGCACT 
TCTTCAACAG TGATTGGAAT CCCTTCCCGA GGTACAAGAT TAGGAACATC TGTGGATGAC 
TCTGTGGATA GCCTGCTACA GCGTATGGCG CAACATGAAA GCCAAGGGAC CTTAGAGAAA 
AACATTGATG CTGTCATTGC CACAGTGTCC ATCCCACCTT CCTCAAGCCC AGGTCATACT 
CATGGTAGGG AACGAAGTCT GGGGAAATCA GACAGTCTGC TAGTACCCAC AGCACCTAGT 
GACTCCTGCA GCAACACCAC CACCACCATC TCACTCCTTT CTGAGAGGTT GCCAAGCAGC 
TGTTCCCCCC ATCATCTCAA AAGGAGTGTG GTAGAAGCCA TGCAACGCCA AGCCCGGAAA 
ATGTGCAATT ATAACAAAAT CTTGGCCACC AAGAAAAACC TAGACCATGT CAATAAAATC 
TTAAAAGCCA AAAAGCTTCA AAGACAGGCA AGGACAGGTA ACAATTTTGT GAAGCGCAGA 
CCAGGCCGAC CCCGGAAATA TCCCCTCCAG GCTGTGGTGT CAATGCAGGC ATTCCAGGCT 
GCCCAATTTG CCAGCCCAGA ATTAGATGAA GGGGAGGAAG GCAGAGCACT ACATCTTGGT 
CCTGATACAG TTACAGATGT CATTGAGGCT GTTGTTCAGA GTGTGAACCT GAACCCAGAA 
CTTAGAAAAG GATGGAAAAG GAAAAGTTGG CTTCTGGAGG AAGAGGCAAG GAAAAGACAG 
AAGATACTTC CAGAGGAAGA ACAGGAAAAT AATAAAAGCT TTCCTGAGAC AACAATTGAA 
TCTCCTAGTC CTCCAGAAGC CCCACCAAAG CCACCTGAAC CTGAAAATAC CCTGCAGCCT 
TTGCCTTCTC TTATCCCTCG GGAAAAGAAG GCCCCACGTC CCCCAAAGAA GAAATATCAG 
AAAGCAGGGC TCTATTCTGA TGTATACAAA ACTACAGATC CAAAGAGCAG ACTGATACAA 
TTAAAGAAAG AAAAACTGGA GTATACTCCT GGAGAACATG AATATGGATT ATTCCCAGCC 
CCTATTCATG TTGGAAAGTA TTTGAGACAA AAGAGAATTG ACTTTCAGCT TCCTTATGAC 
ATCCTCTGGC AATGGAAACA CAATCAGCTA TATAAAAAGC CAGATGTCCC ACTATACAAA 
AAAATCCGTT CAAATGTCTA CGTTGATGTC AAACCTCTTT CTGGCTATGA GGCTACCACC 
TGCAACTGTA AAAAACCAGA TGATGGTGAT AGGAAGGGTT GTATGGATGA CTGCCTCAAC 
AGAATGATCT TTGCTGAGTG TTCCCCCAAT ACCTGCCCCT GTGGAGAGCA GTGCTGCAAT 
CAGAGGATAC AAAGGCATGA GTGGGTACAG TGTCTTGAGC GGTTCCGAGC TGAGGAGAAG 
GGATGGGGGA TCCGGACCAA AGAACCTTTG AAAGCTGGCC AGTTCATTAT TGAATACTTG 
GGAGAAGTTG TCAGTGAGCA GGAGTTCAGA AACCGAATGA TTGAACAGTA CCATAATCAC 
AGTGACCATT ACTGCCTAAA TCTGGACAGT GGAATGGTGA TTGACAGCTA CCGAATGGGC 
AATGAGGCCC GGTTCATCAA CCACAGCTGT AACCCAAATT GTGAAATGCA GAAATGGTCT 
GTTAATGGAG TCTACCGGAT TGGGCTCTAT GCCCTGAAAG ATATGCCAGC TGGTACTGAA 
CTAACCTATG ACTACAATTT TCATTCTTTT AATGTTGAGA AACAGCAACT TTGTAAGTGT 
GGTTTTGACA AATGCCGGGG TATCATCGGA GGCAAGAGCC AACGCATGAA TGGACTCACC 
AGCAAAAGCA GCCAACCTGT GACCACACAC AGACGGCCAG GACGGTCAAA AGAGAAGAGA 
AAATCCAAGC ACAAGTTGAA GAAAAGGAGA GGCCATCTTT CTGAGGAGCC TAATGAAAAT 
GTCAGTACCC CAACCAGAAT GACCCCCCAA CTGCAAATGA AGCCAATGTC CAACCGTGAA 
AGGAACTTTG TGTTAAAACA TCATGTATTC CTGGTCCGGA ACTGGGAGAA AATCAGACAG 
AAGCAAGAGG AGGTAAAACA CACCAGTGAC AATATCCATT CAGCTTCCTT ATATACCCGC 
TGGAATGGTA TCTGCCGAGA TGATGGAAAC ATCAAATCTG ATGTCTTCAT GACCCAGTTC 
TCCGCCTTGC AGACAGCCCG CTCAGTTCGT ACAAGGAGAT TGGCAGCTGC AGAGGAGAAC 
ATTGAAGTGG CCCGGGCAGC CCGCCTGGCC CAGATATTCA AAGAGATATG TGACAGCATC 
ATCTCCTACA AAGATTCCTC CCGACAGGCA TTGGCAACTC CACTGTTGAA CCTTCCCCCA 
AAGAAAAAGA ATGCTGATTA TTATGAGAAG ATATCTGACC CCTTAGATCT CATTACCATT 
GAGAAGCAGA TCCTGACAGG GTATTACAAG ACAGTGGAGG CGTTTGATGC TGACATGCTC 
AAGGTCTTCC GGAATGCTGA GAAATACTAT GGACGCAAGT CCCCAATTGG GAGAGATGTA 
TGTCGACTAC GCAAAGCATA CTACAATGCA CGGCATGAGG CATCAGCTCA AATTGATGAG 
ATTGTGGGGG AAACAGCAAG TGAAGCAGAC AGCAGCGAAA CCTCAGTCTG TGAGAAGGAA 
AATGGGCATG AGAAGGATGA TGATGTTATC CGCTGTATCT GCGGCCTCTA CAAGGATGAA 
GGCCTCATGA TTCAGTGTGA CAAGTGCATG GTGTGGCAGC ACTGTGATTG CATGGGAGTA 
AACTCAGATG TAGAACATTA CCTCTGTGAA CAATGTGATC CAAGGCCTGT GGACAGGGAA 
GTGCCCATGA TACCTCGGCC CCATTATGCC CAACCTGGCT GTGTCTACTT CATCTGTCTA 
CTCCGGGATG ACCTGCTGCT ACGTCAAGGT GACTGTGTGT ACCTGATGCG AGATAGTCGG 
AGAACCCCTG ATGGCCACCC AGTCCGTCAG TCTTATCGAT TGTTATCCCA TATCAACCGA 
GAGAAACTTG ATATCTTTCG AATTGAGAAG CTTTGGAAGA ATGAAAAAGA GGAGCGGTTT 
GCCTTTGGTC ATCATTATTT TCGTCCCCAT GAAACCCACC ACTCCCCATC ACGTCGATTT 
TATCACAATG AGCTGTTCCG AGTGCCACTG TATGAAATCA TTCCTCTGGA GGCTGTGGTG 
GGGACCTGTT GCGTATTAGA TCTGTACACA TATTGTAAAG GAAGGCCCAA AGGAGTGAAA 
GAGCAGGATG TATATATCTG TGACTACCGA CTTGATAAAT CAGCCCACCT GTTCTACAAG 
ATTCATCGGA ACCGCTATCC TGTCTGCACC AAACCCTATG CCTTTGACCA CTTCCCCAAG 
AAGCTCACTC CCAAGAGAGA TTTCTCACCT CATTATGTCC CAGACAACTA CAAGAGGAAT 
GGAGGAAGAT CATCCTGGAA GACAGAACGT TCAAAGCCAC CCATCAAAGA CCTAGGCCAG 
GAGGATGATG CCCTACCACT CATTGAAGAA GTACTGGCCA GTCAAGAGCA AGCAGCCAAT 
GAGATGCCCA CCCTAGAAGA AACTGATCGG GAGGTAGCAC CTGGTGAAAC AACTGAGGGT 
GAAAAGAAAG CTGATGAAAG CAGACAGGAG CCTCAGAGGT CCTGCACACC TGAAGAGAGG 
AGACACAGCC AACGGGAGCG ACTCAACCAG ATTCTGCTCA ACCTTCTTGA GAAGATCCCT 
GGGAAGAATG CCATCGATGT GACATACTTG CTGGAGGAAG GATCAGGAAG AAAGCTTCGA 
AGACGTACTC TGCTTATCCC AGAGAGCAGC TTCCGAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031822580.1
CDS	308..9289
Translation	MDHRNTAMLGVGTDSEGFSRKSPSAVSTGTLVSKREVELENSTKEEEDLWRRNRDRNIETGKNDGLTDAQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTSIKHPRKALKSGKMTDEKNELCPSKRTMSGIYISTLQNNMYVTHWRRGEKDPSKLYKKANDVAAVECRSEEGIHLHSQGENNPVSKKLSPLHSEMTDYVKSAPSTLVGNRDPDSKDRALLNGATSVTEKLAQLIATCPPSKSSKTKPKKLGAGITAGLVSKDLIRKPGIGPMAGIIHKDLIKKPTISPAVGLVTKDPGKKPIFSAAVGLVSKDSGKKLGTGTTAVFINKDLGKKPGTITTVGLVSRESGKKLGIGIVPGLVSKESGKKLGLGTVVGLVNKDSVKKFGPAIGLVAKDCGKKTITNSSVGLVNKDPAKKLVACPVAGLVNKDIVNLKAEALLPVQEPLKVSCNTNTSSHESQEHSESLKDSATSKAFEKTIIRQNKDSLLEKFSIRKEIITLDKDIFNEGTCIQQDSFLSSERGTYVETTKHEKQPPVYCTSPDFQMGGTSDASTAKSPFSAIGESNLPSPSPALSVTPLNRSPPGTSSQLATNPLHLGPSTELIEGIAESIGKTQFSSENSHMNLGHRSLVHSMNTDCKGNDKELNDSKASHTDNSRMGSSIGKKPSLASESSIHATITPSVVSFTSLFSSKPFLKIGAVTASEKHCQVAESLSTSLQSKPLKKRKGRKPRWTKVVARNTCRPSKGLELERSELFKNVSCSSLSNSNSEPAKFMKNIGPSSFVEHDFLKCRLPKLSKSSTAPSLALLTETDKQSHKSFASHKLSSSICMSGDLLPDIYKPKRGRPKTKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQIRSPVKMKPPVLSVAPFVATESPSKLESESDKHRSSSDFFERKDQLQGPDDSHKPSVCGMSDLEMEPDKKINKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSTLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKAVLNGILTSSPASLAVLEKTAHQVAGSALGQILPPLLPSAASSSEVLPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHIHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRSHDYLSYDKMKRQKRKRKKKYPQLRNRQDPDFLAELEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPTFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLAAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPAKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDTLATNSSSRTVLESLKRYRFGKDAVGERYKHKEKHRCHMSCPHLSPSKSLLSREEQWVHREPSESTSLALGLQTPLQIDCSETSPGLSLGGFTPSSEPASSDEHTNLFTSAIGSCRVSNSTNTSGRKKLTDSPGLFTAQDTSLNRPLRKETLPSNERALQTLTGSQPVLDKPSQRPSESTNCSPTRKRSSSESTSSTVIGIPSRGTRLGTSVDDSVDSLLQRMAQHESQGTLEKNIDAVIATVSIPPSSSPGHTHGRERSLGKSDSLLVPTAPSDSCSNTTTTISLLSERLPSSCSPHHLKRSVVEAMQRQARKMCNYNKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKYPLQAVVSMQAFQAAQFASPELDEGEEGRALHLGPDTVTDVIEAVVQSVNLNPELRKGWKRKSWLLEEEARKRQKILPEEEQENNKSFPETTIESPSPPEAPPKPPEPENTLQPLPSLIPREKKAPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPDDGDRKGCMDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCNPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFDKCRGIIGGKSQRMNGLTSKSSQPVTTHRRPGRSKEKRKSKHKLKKRRGHLSEEPNENVSTPTRMTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIRQKQEEVKHTSDNIHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENIEVARAARLAQIFKEICDSIISYKDSSRQALATPLLNLPPKKKNADYYEKISDPLDLITIEKQILTGYYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKAYYNARHEASAQIDEIVGETASEADSSETSVCEKENGHEKDDDVIRCICGLYKDEGLMIQCDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINREKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKTERSKPPIKDLGQEDDALPLIEEVLASQEQAANEMPTLEETDREVAPGETTEGEKKADESRQEPQRSCTPEERRHSQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLLIPESSFRK

Target ORF information:

RefSeq Version	XM_031966720.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031966720.1

ORF Insert Sequence:

ATGGACCATA GGAATACTGC TATGTTAGGA GTGGGTACCG ACTCCGAAGG ATTTTCAAGG 
AAGAGTCCTT CTGCTGTCAG CACTGGAACA CTGGTTAGTA AGAGAGAAGT GGAACTAGAG 
AATAGCACTA AGGAAGAAGA GGATCTTTGG AGGCGGAATA GGGACAGAAA CATTGAGACA 
GGGAAAAATG ATGGTTTGAC TGATGCACAG CAGTTTTCTG TGAAAGAAAC AAACTTTTCA 
GAGGGAAACT TAAAGTTGAA AATTGGCCTT CAGGCTAAGA GAACTAAAAA GCCTCCTAAA 
AACTTGGAGA ACTATGTATG TCGACCTGCT ATAAAAACAA GCATTAAGCA TCCAAGGAAA 
GCATTGAAAA GTGGGAAGAT GACAGATGAA AAAAATGAAC TTTGCCCTTC AAAACGAACT 
ATGTCGGGAA TATATATCTC CACATTGCAA AACAACATGT ATGTTACACA TTGGAGGAGG 
GGAGAAAAAG ACCCTTCAAA ACTTTACAAG AAAGCAAATG ATGTTGCAGC TGTTGAATGC 
CGCTCTGAAG AAGGCATCCA CCTTCATTCA CAGGGAGAAA ATAATCCTGT GTCAAAGAAG 
CTTTCTCCAC TACACTCTGA AATGACAGAT TATGTTAAAT CAGCACCATC TACTCTTGTT 
GGTAACCGTG ATCCTGACTC GAAGGACAGA GCTCTACTTA ATGGAGCAAC AAGTGTAACA 
GAAAAATTGG CACAGCTCAT TGCTACTTGT CCACCCTCCA AGTCTTCCAA GACAAAGCCA 
AAAAAGTTAG GGGCTGGTAT TACAGCAGGA TTGGTTAGCA AAGACTTGAT CAGAAAGCCA 
GGCATTGGTC CTATGGCAGG AATTATTCAC AAGGACTTGA TTAAGAAACC AACAATTAGC 
CCAGCAGTTG GGTTGGTAAC TAAAGACCCT GGGAAAAAGC CAATCTTTAG TGCTGCAGTA 
GGATTGGTCA GTAAAGACTC TGGGAAAAAA TTAGGAACTG GGACTACTGC GGTATTCATA 
AACAAGGACT TAGGAAAGAA ACCAGGGACT ATCACTACAG TGGGACTGGT GAGCAGAGAG 
TCAGGGAAGA AATTAGGAAT TGGTATCGTC CCAGGTTTGG TGAGTAAAGA ATCAGGAAAG 
AAGTTGGGAC TTGGTACTGT AGTTGGGCTT GTTAATAAAG ATTCAGTAAA GAAGTTTGGC 
CCTGCCATTG GACTGGTTGC AAAGGACTGT GGGAAGAAGA CTATAACAAA TTCATCAGTG 
GGATTGGTTA ATAAGGACCC TGCAAAGAAA TTGGTGGCTT GCCCTGTAGC AGGATTGGTC 
AACAAAGACA TTGTGAATTT AAAAGCTGAA GCCTTGCTCC CTGTTCAAGA ACCACTTAAG 
GTTTCTTGCA ATACAAATAC TAGTAGTCAT GAAAGTCAGG AGCATTCTGA ATCCCTGAAA 
GATAGTGCCA CCAGCAAAGC TTTTGAGAAG ACTATTATTC GTCAGAATAA AGATAGTTTA 
TTAGAAAAGT TCTCAATTCG AAAAGAAATC ATTACTTTGG ACAAAGACAT TTTTAATGAA 
GGAACTTGCA TACAGCAGGA CAGTTTCTTA TCCAGTGAAA GGGGGACCTA TGTTGAAACT 
ACAAAGCATG AGAAGCAGCC TCCAGTGTAT TGCACTTCAC CAGATTTTCA AATGGGAGGC 
ACTTCTGATG CATCAACAGC TAAGTCTCCC TTTAGTGCAA TAGGGGAGAG TAATCTCCCT 
TCCCCTTCAC CAGCCTTGTC TGTTACGCCC TTAAACAGAA GTCCCCCTGG AACTTCTTCT 
CAGTTGGCTA CTAATCCATT ACATTTAGGT CCTTCCACAG AACTCATAGA AGGAATTGCT 
GAATCTATTG GAAAAACCCA ATTCAGTTCT GAAAATTCCC ATATGAACCT TGGTCACAGG 
TCTCTGGTTC ACAGCATGAA CACTGATTGC AAAGGAAATG ATAAAGAGTT AAATGATTCA 
AAAGCTTCTC ACACAGATAA CTCAAGAATG GGCTCCTCCA TAGGAAAAAA GCCAAGTCTG 
GCTTCAGAAT CCAGTATTCA TGCTACAATT ACTCCCTCAG TTGTTAGTTT CACTAGTTTA 
TTTAGCAGTA AACCCTTTCT GAAGATTGGT GCAGTAACTG CATCAGAGAA ACACTGCCAA 
GTTGCTGAAA GCCTGAGCAC TAGTTTGCAG TCCAAACCAT TAAAAAAAAG AAAAGGAAGG 
AAACCACGAT GGACTAAAGT GGTAGCAAGA AACACATGCC GGCCTTCAAA AGGGCTAGAA 
TTAGAAAGGT CAGAGCTTTT TAAAAATGTT TCATGTAGCT CTTTGTCAAA TAGCAATTCC 
GAGCCAGCCA AGTTTATGAA AAACATTGGG CCATCTTCAT TTGTGGAGCA TGACTTCCTT 
AAATGCCGAT TGCCAAAGCT GAGTAAATCT AGCACAGCTC CATCTCTTGC TCTCTTAACT 
GAAACTGACA AGCAGTCTCA TAAGTCCTTT GCCAGCCACA AACTTTCCTC TAGTATATGC 
ATGTCTGGTG ACCTTTTACC TGACATTTAT AAACCTAAAA GAGGAAGACC TAAAACTAAG 
GAGATGCCCC AACTAGAAGG TCCACCCAAA AGGACCCTAA AAATCCCGGC CTCTAAAGTT 
TTTTCATTAC AGTCAAAAGA GGAGCAAGAA CCCCCTATTT TGCAGCCAGA AATTGAAATC 
CCTTCCTTCA AGCAGAGCCT ATCAGTATCA CCCTTTCCCA AAAAAAGAGG AAGGCCAAAA 
AGACAAATCA GATCACCAGT CAAGATGAAA CCACCTGTGC TATCAGTGGC ACCCTTTGTA 
GCCACTGAAA GTCCAAGTAA ACTAGAGTCT GAGAGTGATA AACATAGAAG TAGTAGTGAC 
TTCTTTGAAA GGAAAGATCA ACTTCAGGGT CCAGATGACA GTCATAAACC AAGTGTGTGT 
GGTATGAGTG ACCTTGAGAT GGAACCTGAT AAAAAAATTA ATAAGAGAAA CAATGGCCAA 
TTAATGAAAA CTATCATTCG CAAAATAAAT AAGATGAAGA CTTTGAAGAG AAAGAAACTG 
CTGAATCAGA TTCTCTCAAG TTCTGTTGAA TCAAGTAACA AAGGGAAAGT GCAATCCAAA 
CTCCACAATA CAGTGTCAAC ACTTGCTGCC ACTTTTGGCT CCAAACTGGG CCAACAGATA 
AATGTCAGCA AGAAAGGAAC CATTTATATA GGAAAGAGGC GAGGCCGGAA ACCAAAAGCT 
GTGCTGAACG GCATCTTAAC TAGTAGCCCA GCTAGTCTGG CTGTCCTAGA GAAAACAGCT 
CATCAGGTAG CTGGGTCAGC ATTAGGACAG ATTCTCCCCC CACTCTTGCC TTCAGCAGCA 
AGTAGTTCTG AGGTTCTTCC ATCCCCCATC TGTTCTCAGT CTTCTGGGAC TAGTGGGGGC 
CAGAGTCCTG TCAGCAGTGA TGCAGGATTT GTTGAGCCCA GTTCAGTGCC ATATTTACAT 
ATACACTCCA GACAGGGCAG TATGATTCAG ACACTTGCTA TGAAGAAGGC TTCAAAGGGA 
AGGAGACGGT TGTCTCCTCC TACTTTGTTG CCAAATTCTC CTTCTCACTT GAGTGAACTC 
ACTTCTCTGA AGGAAGCTAC TCCTTCCCCA ATTAGTGAAT CTCATAGTGA TGAGACCATT 
CCTAGTGATA GTGGAATTGG AACAGATAAT AATAGCACAT CAGACAGGGC AGAAAAGTTT 
TGTGGGCAGA AAAAGAGGAG GCATTCTTTT GAACATGTTT CTCTCATTCC CCCTGAAACC 
TCCACAGTCC TAAGTAGTCT CAAAGAGAAA CATAAACACA AATGTAAACG CAGGAGTCAT 
GATTACCTCA GTTATGACAA AATGAAAAGA CAGAAGCGAA AACGGAAAAA GAAGTATCCC 
CAGTTACGAA ACAGACAGGA TCCAGACTTT CTTGCTGAGC TGGAGGAATT AATAAGTCGT 
TTAAGTGAAA TTCGGATCAC TCATCGAAGT CATCATTTTA TTCCCCGTGA TCTCCTACCC 
ACTATTTTTC GAATTAACTT TAACAGTTTC TATACGCACC CCACTTTTCC CTTAGACCCT 
TTGCACTACA TTAGAAAACC TGACTTAAAG AAAAAAAGAG GGAGGCCTCC CAAGATGAGG 
GAAGCAATGG CTGAAATGCC TTTTATGCAT AGCCTTAGTT TCCCTCTCTC TAGTACTGGG 
TTTTACCCCT CTTATGGCAT GCCTTATTCT CCCTCTCCAC TTGCAGCTGC TCCCATAGGA 
TTAGGATACT ATGGAAGGTA CCCTCCAACT CTTTATCCAC CACCCCCCTC TCCTTCTTTC 
ACCACTCCTT TGCCACCTCC TTCTTACATG CATGCTGGTC ATTTGCTTCT CAATCCTGCC 
AAGTACCACA AGAAGAAACA TAAGCTGCTT CGTCAGGAAG CCTTTCTTAC AACAAGTAGG 
ACACCCCTTC TCTCCATGAG CACCTACCCC AGTGTCCCTC CTGAGATGGC CTATGGTTGG 
ATGGTGGAGC ATAAACACCG GCACCGGCAC AAACACAGAG AACACCGTTC TTCTGAGCAA 
CCCCAGGTTT CCATGGACAC TTTAGCTACC AATAGCTCTT CCAGAACTGT CCTAGAATCG 
TTGAAGCGCT ATCGATTTGG TAAAGATGCT GTTGGGGAGC GATATAAACA CAAAGAGAAG 
CATCGTTGTC ACATGTCCTG TCCTCATCTG TCCCCATCAA AAAGCTTATT AAGCCGAGAG 
GAGCAATGGG TCCACCGAGA GCCTTCAGAA TCCACTTCAT TGGCCTTGGG GTTGCAGACA 
CCTTTACAGA TTGACTGTTC AGAAACGTCT CCAGGTTTGT CCCTGGGAGG TTTTACACCT 
AGCTCTGAGC CAGCCAGCAG TGATGAACAC ACAAACCTTT TCACAAGTGC AATAGGCAGC 
TGCAGAGTAT CAAACTCTAC TAATACAAGT GGCCGGAAGA AATTGACTGA CAGTCCAGGA 
CTCTTTACCG CACAGGACAC TTCATTAAAT CGGCCTCTTA GAAAGGAAAC TTTGCCTTCC 
AATGAAAGGG CACTGCAAAC TTTGACAGGC TCCCAGCCAG TCCTAGACAA GCCTTCCCAA 
AGGCCATCAG AGAGCACAAA CTGTAGCCCT ACTCGAAAGA GATCTTCATC AGAGAGCACT 
TCTTCAACAG TGATTGGAAT CCCTTCCCGA GGTACAAGAT TAGGAACATC TGTGGATGAC 
TCTGTGGATA GCCTGCTACA GCGTATGGCG CAACATGAAA GCCAAGGGAC CTTAGAGAAA 
AACATTGATG CTGTCATTGC CACAGTGTCC ATCCCACCTT CCTCAAGCCC AGGTCATACT 
CATGGTAGGG AACGAAGTCT GGGGAAATCA GACAGTCTGC TAGTACCCAC AGCACCTAGT 
GACTCCTGCA GCAACACCAC CACCACCATC TCACTCCTTT CTGAGAGGTT GCCAAGCAGC 
TGTTCCCCCC ATCATCTCAA AAGGAGTGTG GTAGAAGCCA TGCAACGCCA AGCCCGGAAA 
ATGTGCAATT ATAACAAAAT CTTGGCCACC AAGAAAAACC TAGACCATGT CAATAAAATC 
TTAAAAGCCA AAAAGCTTCA AAGACAGGCA AGGACAGGTA ACAATTTTGT GAAGCGCAGA 
CCAGGCCGAC CCCGGAAATA TCCCCTCCAG GCTGTGGTGT CAATGCAGGC ATTCCAGGCT 
GCCCAATTTG CCAGCCCAGA ATTAGATGAA GGGGAGGAAG GCAGAGCACT ACATCTTGGT 
CCTGATACAG TTACAGATGT CATTGAGGCT GTTGTTCAGA GTGTGAACCT GAACCCAGAA 
CTTAGAAAAG GATGGAAAAG GAAAAGTTGG CTTCTGGAGG AAGAGGCAAG GAAAAGACAG 
AAGATACTTC CAGAGGAAGA ACAGGAAAAT AATAAAAGCT TTCCTGAGAC AACAATTGAA 
TCTCCTAGTC CTCCAGAAGC CCCACCAAAG CCACCTGAAC CTGAAAATAC CCTGCAGCCT 
TTGCCTTCTC TTATCCCTCG GGAAAAGAAG GCCCCACGTC CCCCAAAGAA GAAATATCAG 
AAAGCAGGGC TCTATTCTGA TGTATACAAA ACTACAGATC CAAAGAGCAG ACTGATACAA 
TTAAAGAAAG AAAAACTGGA GTATACTCCT GGAGAACATG AATATGGATT ATTCCCAGCC 
CCTATTCATG TTGGAAAGTA TTTGAGACAA AAGAGAATTG ACTTTCAGCT TCCTTATGAC 
ATCCTCTGGC AATGGAAACA CAATCAGCTA TATAAAAAGC CAGATGTCCC ACTATACAAA 
AAAATCCGTT CAAATGTCTA CGTTGATGTC AAACCTCTTT CTGGCTATGA GGCTACCACC 
TGCAACTGTA AAAAACCAGA TGATGGTGAT AGGAAGGGTT GTATGGATGA CTGCCTCAAC 
AGAATGATCT TTGCTGAGTG TTCCCCCAAT ACCTGCCCCT GTGGAGAGCA GTGCTGCAAT 
CAGAGGATAC AAAGGCATGA GTGGGTACAG TGTCTTGAGC GGTTCCGAGC TGAGGAGAAG 
GGATGGGGGA TCCGGACCAA AGAACCTTTG AAAGCTGGCC AGTTCATTAT TGAATACTTG 
GGAGAAGTTG TCAGTGAGCA GGAGTTCAGA AACCGAATGA TTGAACAGTA CCATAATCAC 
AGTGACCATT ACTGCCTAAA TCTGGACAGT GGAATGGTGA TTGACAGCTA CCGAATGGGC 
AATGAGGCCC GGTTCATCAA CCACAGCTGT AACCCAAATT GTGAAATGCA GAAATGGTCT 
GTTAATGGAG TCTACCGGAT TGGGCTCTAT GCCCTGAAAG ATATGCCAGC TGGTACTGAA 
CTAACCTATG ACTACAATTT TCATTCTTTT AATGTTGAGA AACAGCAACT TTGTAAGTGT 
GGTTTTGACA AATGCCGGGG TATCATCGGA GGCAAGAGCC AACGCATGAA TGGACTCACC 
AGCAAAAGCA GCCAACCTGT GACCACACAC AGACGGCCAG GACGGTCAAA AGAGAAGAGA 
AAATCCAAGC ACAAGTTGAA GAAAAGGAGA GGCCATCTTT CTGAGGAGCC TAATGAAAAT 
GTCAGTACCC CAACCAGAAT GACCCCCCAA CTGCAAATGA AGCCAATGTC CAACCGTGAA 
AGGAACTTTG TGTTAAAACA TCATGTATTC CTGGTCCGGA ACTGGGAGAA AATCAGACAG 
AAGCAAGAGG AGGTAAAACA CACCAGTGAC AATATCCATT CAGCTTCCTT ATATACCCGC 
TGGAATGGTA TCTGCCGAGA TGATGGAAAC ATCAAATCTG ATGTCTTCAT GACCCAGTTC 
TCCGCCTTGC AGACAGCCCG CTCAGTTCGT ACAAGGAGAT TGGCAGCTGC AGAGGAGAAC 
ATTGAAGTGG CCCGGGCAGC CCGCCTGGCC CAGATATTCA AAGAGATATG TGACAGCATC 
ATCTCCTACA AAGATTCCTC CCGACAGGCA TTGGCAACTC CACTGTTGAA CCTTCCCCCA 
AAGAAAAAGA ATGCTGATTA TTATGAGAAG ATATCTGACC CCTTAGATCT CATTACCATT 
GAGAAGCAGA TCCTGACAGG GTATTACAAG ACAGTGGAGG CGTTTGATGC TGACATGCTC 
AAGGTCTTCC GGAATGCTGA GAAATACTAT GGACGCAAGT CCCCAATTGG GAGAGATGTA 
TGTCGACTAC GCAAAGCATA CTACAATGCA CGGCATGAGG CATCAGCTCA AATTGATGAG 
ATTGTGGGGG AAACAGCAAG TGAAGCAGAC AGCAGCGAAA CCTCAGTCTG TGAGAAGGAA 
AATGGGCATG AGAAGGATGA TGATGTTATC CGCTGTATCT GCGGCCTCTA CAAGGATGAA 
GGCCTCATGA TTCAGTGTGA CAAGTGCATG GTGTGGCAGC ACTGTGATTG CATGGGAGTA 
AACTCAGATG TAGAACATTA CCTCTGTGAA CAATGTGATC CAAGGCCTGT GGACAGGGAA 
GTGCCCATGA TACCTCGGCC CCATTATGCC CAACCTGGCT GTGTCTACTT CATCTGTCTA 
CTCCGGGATG ACCTGCTGCT ACGTCAAGGT GACTGTGTGT ACCTGATGCG AGATAGTCGG 
AGAACCCCTG ATGGCCACCC AGTCCGTCAG TCTTATCGAT TGTTATCCCA TATCAACCGA 
GAGAAACTTG ATATCTTTCG AATTGAGAAG CTTTGGAAGA ATGAAAAAGA GGAGCGGTTT 
GCCTTTGGTC ATCATTATTT TCGTCCCCAT GAAACCCACC ACTCCCCATC ACGTCGATTT 
TATCACAATG AGCTGTTCCG AGTGCCACTG TATGAAATCA TTCCTCTGGA GGCTGTGGTG 
GGGACCTGTT GCGTATTAGA TCTGTACACA TATTGTAAAG GAAGGCCCAA AGGAGTGAAA 
GAGCAGGATG TATATATCTG TGACTACCGA CTTGATAAAT CAGCCCACCT GTTCTACAAG 
ATTCATCGGA ACCGCTATCC TGTCTGCACC AAACCCTATG CCTTTGACCA CTTCCCCAAG 
AAGCTCACTC CCAAGAGAGA TTTCTCACCT CATTATGTCC CAGACAACTA CAAGAGGAAT 
GGAGGAAGAT CATCCTGGAA GACAGAACGT TCAAAGCCAC CCATCAAAGA CCTAGGCCAG 
GAGGATGATG CCCTACCACT CATTGAAGAA GTACTGGCCA GTCAAGAGCA AGCAGCCAAT 
GAGATGCCCA CCCTAGAAGA AACTGATCGG GAGGTAGCAC CTGGTGAAAC AACTGAGGGT 
GAAAAGAAAG CTGATGAAAG CAGACAGGAG CCTCAGAGGT CCTGCACACC TGAAGAGAGG 
AGACACAGCC AACGGGAGCG ACTCAACCAG ATTCTGCTCA ACCTTCTTGA GAAGATCCCT 
GGGAAGAATG CCATCGATGT GACATACTTG CTGGAGGAAG GATCAGGAAG AAAGCTTCGA 
AGACGTACTC TGCTTATCCC AGAGAGCAGC TTCCGAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ASH1L cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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