VARS cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	VARS
Entrez Gene ID	100929586
Full Name	valyl-tRNA synthetase
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031965157.1	XP_031821017.1	valine--tRNA ligase
XM_012548900.1	XP_012404354.1	valine--tRNA ligase
XM_031965158.1	XP_031821018.1	valine--tRNA ligase
XM_031965159.1	XP_031821019.1	valine--tRNA ligase
XM_023502899.1	XP_023358667.1	valine--tRNA ligase

Pathways from BioSystems
Gene Ontology

The following VARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa131011	XM_012548900.1 Latest version!	Sarcophilus harrisii valyl-tRNA synthetase (VARS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa154727	XM_023502899.1 Latest version!	Sarcophilus harrisii valyl-tRNA synthetase (VARS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa233182	XM_031965159.1 Latest version!	Sarcophilus harrisii valyl-tRNA synthetase 1 (VARS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa233182	XM_031965157.1 Latest version!	Sarcophilus harrisii valyl-tRNA synthetase 1 (VARS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa233182	XM_031965158.1 Latest version!	Sarcophilus harrisii valyl-tRNA synthetase 1 (VARS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VARS ( XM_012548900.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012404354.1 Sarcophilus harrisii valyl-tRNA synthetase (VARS), mRNA.

Price & Availability↑

CloneID	OSa131011
Clone ID Related Accession (Same CDS sequence)	XM_012548900.1
Accession Version	XM_012548900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCCATCC TGTATGTGTC TCCTCACCCG GACGCCTTCC CCAGCCTTCG CGCCCTGATT 
GCTGCCCGAT ACGGGGAGTC TGGAACTGGC CCAGGGTGGG GAGGGCCTCC TCCCCGCGTC 
TGCCTCCAGG CACCCCCTAC CAGCGGCACT TCCATCCCCC CGCCTCGGCT GCCGATTTCT 
TGTAACTATA ATTCCGCTGC TGCCTTCCTT TTTAACCCTT TGTTTCCTCC CTCTTCTCAG 
GCAGCCCTGG GAGCCCTGGG ACGGGCTCTG GGCCCATTGG AGGAGAGACT GAGGTTGCAT 
ACCTATTTGG TAGGAGAAGC CCCCACTTTG GCCGACCTGG CAGCTGTTAC AGCCCTACTG 
CTGCCCTTCC GCTATGTTCT AGACCCTCCT GCTCGCTGGG CTTGGGGTAA CGTAAGCCGC 
TGGTTCATGA CATGTGTCCA GCAACCAGAA TTTCGTGCTG TGTTAGGGGA TGTGTCCCTG 
TGCCCAGGAG TGCGGTCTCT CCCACAGCAA CCTGGCTCTG AGGTTTCTGG GTCCTCCAAG 
ACAGCAGCCC AGTTGAAAAA AGAAGCAAAA AAGCGGGAAA AGCTGGAGAA GTTTCAACAG 
AAGCAAAGGA ACCAATTGCA TCAGCCACCT CCTGGAGAGA AGAAGCCAAA AATTGAGAAG 
AAGGAGAAAC GGGACCCTGG GGTCATAACA TATGATATCC CCACCCCACC AGGGGAGAAG 
AAAGATGTAA GTGTGTCCAT GCCTGACTCC TATAGTCCCC AGTATGTTGA AGCTGCTTGG 
TATTCATGGT GGGAGAGCCA AGGATTCTTC AAACCAGAGT ATGGGCGTGC CAGTGTGTCA 
GAGCCTAATC CTCGAGGTGT CTTCATGATG TGCATCCCGC CTCCCAATGT GACAGGGTCC 
CTGCACCTTG GCCATGCCCT CACCAATGCC ATCCAGGATT CACTGACCCG CTGGCACCGA 
ATGAGAGGAG AAACAACCCT GTGGAACCCA GGCTGTGATC ATGCTGGTAT TGCCACACAG 
GTAGTTGTGG AAAAGAAACT ATGGAGGGAA CATGGGCTAA GCCGCCACCA GCTTGGTCGA 
GAGGCCTTCC TACAAGAGGT CTGGAAATGG AAGAATGAGA AGGGAGACCG GATTTACCAT 
CAGCTAAGGA AGCTTGGTGG CTCCTTGGAT TGGGATCGAG CTTGTTTCAC CATGGATCCT 
AAACTCTCAG CAGCTGTGAC AGAAGCTTTT GTTCGCCTCC ATAATGATGG GGTCATTTAC 
CGGAGTACCC GCCTTGTCAA CTGGTCCTGT AGTCTCAACT CTGCCATTTC TGACATTGAG 
GTAGATAAGA AGGAACTGTC GGGCCGAACC TTGCTTTCTG TCCCAGGCTA TCAGGAGAAG 
GTGGAATTTG GGGTCATTGT TTCTTTTGCC TACAAGATAC AGGATTCAGA GAGCAATGAG 
GAGGTGGTCG TAGCAACTAC TCGTATTGAG ACCATGTTGG GAGATGTAGC TGTGGCTGTG 
CACCCCAATG ACCCTAGATA CCAGCACCTT CAGGGAAAGA GTGTTATACA CCCATTCCTT 
GCTCGAAGCC TTCCTATCAT CTTTGATGAA TTTGTGGACA TGGAGTTTGG CACTGGTGCT 
GTAAAAATTA CACCAGCCCA TGATCAGAAT GACTATGAGG TTGGGCAAAG ACACAAGCTA 
GAAGCAGTGA GCATCATAGA CCACCAAGGC AACCTTATCA ATGTTCCGCC TCCCTTTCTG 
GGCCTTCCTC GGTTTGAAGC CAGGAAAGCA GTGTTGGCAG CCCTAAAAGA CAAGGGACTA 
TTCCGGGAAG TGAAGGACAA TCCAATGGTG GTACCTTTGT GCAACCGCTC CAAAGATGTC 
GTTGAACCCC TCCTTAAGCC CCAGTGGTAT GTGAGGTGTG GTGAAATGGC TCAGGCAGCC 
AGTGCAGCAG TGACTCGGGG TGACCTTAAG ATATTACCTG AGATTCATCA GAAAATATGG 
CACAATTGGA TGGACAACAT CAGAGACTGG TGCATCTCCC GGCAGCTGTG GTGGGGTCAC 
CGAATCCCTG CCTACTTTGT CACTGTCAAT GACCCTAAAG TGCCCCCTGG GGAGGATCCT 
GATGGTCGGT ATTGGGTGAG TGGCCGAAGT GAAGAGGAGG CGAGGGAGAA GGCAGCAAAG 
AAGTTTGGAG TGTCCACAGA CAAGATTAGC CTCTCACAGG ATGAAGATGT TTTAGACACA 
TGGTTTTCCT CTGGCATTTT TCCCTTTTCC ATCTTTGGAT GGCCCAACCA GACAGAGGAC 
TTAAGTATAT TCTACCCAGG GACCTTGTTG GAGACAGGAC ATGACATTTT ATTCTTCTGG 
GTAGCCCGCA TGGTGATGCT GGGTCTGAAG CTAACAGGAA AACTGCCCTT CAAAGAGGTT 
TATCTCCATG CCCTCGTCAG AGATGCCCAT GGCCGGAAAA TGAGCAAATC ACTTGGTAAT 
GTCATTGACC CCCTGGATGT CATCAGTGGA CTCTCCCTGC AGGGACTGCA TGATCAGCTA 
CTGAACAGCA ACCTTGACCC CAGTGAAATG GAGAAAGCCA AGGAGGGGCA GAAGGCAGAT 
TTCCCCAATG GAATCCCTGA ATGTGGTACA GATGCTCTCA GATTTGGACT TTGTGCTTAC 
ACTTCTCAGG GCCGGGACAT TAACCTGGAT GTGAACCGAA TCCTTGGGTA TCGACATTTC 
TGTAATAAGC TCTGGAATGC CACCAAGTTT GCCCTTAGGG CATTGGGAGA TGGATTTGTA 
CCCTCACCTA CTCCCCAAGC TAGCAATCAG GAGAGCTTGG CTGATCGTTG GATTAGGAGC 
CGCCTGAGTG AAGCTGTTGC GCTGAGCGAC CAGGGTTTCC ATGCCTACGA TTTCCCTACT 
GTCACCACTG CCCAGTATAG CTTTTGGCTC TATGACCTCT GCAATGTCTA CCTGGAGTGC 
TTGAAACCTG TGTTGTCTGG GACAGACCAA GCTGCAGCAG ATACTGCCCG GCAGACCCTC 
TATACTTGCC TGGATGTGGG CCTAAGACTA CTCTCACCCT TCATGCCTTT TGTGACTGAG 
GAACTGTATC AGCGATTGCC GCGGCGGGGG CCCCAGATGC CACCCAGTCT TTGTGTTACC 
CCCTACCCAG AGCCCACTGA GTTCTCCTGG AAGGATCCCG AGGCTGAGGC TGCTTTTGAG 
CTTGCACTTA GTATTATCCG GGCTGTGCGT TCTCTCCGAG CTGATTATAA CCTCACCCGA 
AGCCAACCTG AATGTTTTCT GGAAGTGGTG GATGAGGCAA CAGGAGTACA GGCTTCTACA 
GTGTCAGGCT ATGTGCAGGC TCTCTCCAAC ACTGGAGCTG TGACTATTCT GCTCCCTGGG 
TCCCCTGCTC CACAGGGTTG TGCTGTGGGC CTGGCCTCTG ACCGATGCTC TGTCCACCTG 
CAGCTTCAGG GGCTGGTGGA TCCTTCCCGG GAGCTGGCAA AACTCCGGGC CAAACAAGGG 
GAAGCTGAGC ATCAGGCCCA AAGGCTTCGG GAGCGTCGGG CTGTTCCTGA TTATACCACC 
AAGGTTCCTT TCCAGGTCCA GGAGTCTGAG GAAGCTAAGC TCCAACAGAC AGAGGCAGAG 
CTGAAGAAGG TAGATGAAGC TATTGCCCTG TTTGAGAAGA TGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012404354.1
CDS	159..3806
Translation	MSILYVSPHPDAFPSLRALIAARYGESGTGPGWGGPPPRVCLQAPPTSGTSIPPPRLPISCNYNSAAAFLFNPLFPPSSQAALGALGRALGPLEERLRLHTYLVGEAPTLADLAAVTALLLPFRYVLDPPARWAWGNVSRWFMTCVQQPEFRAVLGDVSLCPGVRSLPQQPGSEVSGSSKTAAQLKKEAKKREKLEKFQQKQRNQLHQPPPGEKKPKIEKKEKRDPGVITYDIPTPPGEKKDVSVSMPDSYSPQYVEAAWYSWWESQGFFKPEYGRASVSEPNPRGVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREHGLSRHQLGREAFLQEVWKWKNEKGDRIYHQLRKLGGSLDWDRACFTMDPKLSAAVTEAFVRLHNDGVIYRSTRLVNWSCSLNSAISDIEVDKKELSGRTLLSVPGYQEKVEFGVIVSFAYKIQDSESNEEVVVATTRIETMLGDVAVAVHPNDPRYQHLQGKSVIHPFLARSLPIIFDEFVDMEFGTGAVKITPAHDQNDYEVGQRHKLEAVSIIDHQGNLINVPPPFLGLPRFEARKAVLAALKDKGLFREVKDNPMVVPLCNRSKDVVEPLLKPQWYVRCGEMAQAASAAVTRGDLKILPEIHQKIWHNWMDNIRDWCISRQLWWGHRIPAYFVTVNDPKVPPGEDPDGRYWVSGRSEEEAREKAAKKFGVSTDKISLSQDEDVLDTWFSSGIFPFSIFGWPNQTEDLSIFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFKEVYLHALVRDAHGRKMSKSLGNVIDPLDVISGLSLQGLHDQLLNSNLDPSEMEKAKEGQKADFPNGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRALGDGFVPSPTPQASNQESLADRWIRSRLSEAVALSDQGFHAYDFPTVTTAQYSFWLYDLCNVYLECLKPVLSGTDQAAADTARQTLYTCLDVGLRLLSPFMPFVTEELYQRLPRRGPQMPPSLCVTPYPEPTEFSWKDPEAEAAFELALSIIRAVRSLRADYNLTRSQPECFLEVVDEATGVQASTVSGYVQALSNTGAVTILLPGSPAPQGCAVGLASDRCSVHLQLQGLVDPSRELAKLRAKQGEAEHQAQRLRERRAVPDYTTKVPFQVQESEEAKLQQTEAELKKVDEAIALFEKML

Target ORF information:

RefSeq Version	XM_012548900.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii valyl-tRNA synthetase (VARS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012548900.1

ORF Insert Sequence:

ATGTCCATCC TGTATGTGTC TCCTCACCCG GACGCCTTCC CCAGCCTTCG CGCCCTGATT 
GCTGCCCGAT ACGGGGAGTC TGGAACTGGC CCAGGGTGGG GAGGGCCTCC TCCCCGCGTC 
TGCCTCCAGG CACCCCCTAC CAGCGGCACT TCCATCCCCC CGCCTCGGCT GCCGATTTCT 
TGTAACTATA ATTCCGCTGC TGCCTTCCTT TTTAACCCTT TGTTTCCTCC CTCTTCTCAG 
GCAGCCCTGG GAGCCCTGGG ACGGGCTCTG GGCCCATTGG AGGAGAGACT GAGGTTGCAT 
ACCTATTTGG TAGGAGAAGC CCCCACTTTG GCCGACCTGG CAGCTGTTAC AGCCCTACTG 
CTGCCCTTCC GCTATGTTCT AGACCCTCCT GCTCGCTGGG CTTGGGGTAA CGTAAGCCGC 
TGGTTCATGA CATGTGTCCA GCAACCAGAA TTTCGTGCTG TGTTAGGGGA TGTGTCCCTG 
TGCCCAGGAG TGCGGTCTCT CCCACAGCAA CCTGGCTCTG AGGTTTCTGG GTCCTCCAAG 
ACAGCAGCCC AGTTGAAAAA AGAAGCAAAA AAGCGGGAAA AGCTGGAGAA GTTTCAACAG 
AAGCAAAGGA ACCAATTGCA TCAGCCACCT CCTGGAGAGA AGAAGCCAAA AATTGAGAAG 
AAGGAGAAAC GGGACCCTGG GGTCATAACA TATGATATCC CCACCCCACC AGGGGAGAAG 
AAAGATGTAA GTGTGTCCAT GCCTGACTCC TATAGTCCCC AGTATGTTGA AGCTGCTTGG 
TATTCATGGT GGGAGAGCCA AGGATTCTTC AAACCAGAGT ATGGGCGTGC CAGTGTGTCA 
GAGCCTAATC CTCGAGGTGT CTTCATGATG TGCATCCCGC CTCCCAATGT GACAGGGTCC 
CTGCACCTTG GCCATGCCCT CACCAATGCC ATCCAGGATT CACTGACCCG CTGGCACCGA 
ATGAGAGGAG AAACAACCCT GTGGAACCCA GGCTGTGATC ATGCTGGTAT TGCCACACAG 
GTAGTTGTGG AAAAGAAACT ATGGAGGGAA CATGGGCTAA GCCGCCACCA GCTTGGTCGA 
GAGGCCTTCC TACAAGAGGT CTGGAAATGG AAGAATGAGA AGGGAGACCG GATTTACCAT 
CAGCTAAGGA AGCTTGGTGG CTCCTTGGAT TGGGATCGAG CTTGTTTCAC CATGGATCCT 
AAACTCTCAG CAGCTGTGAC AGAAGCTTTT GTTCGCCTCC ATAATGATGG GGTCATTTAC 
CGGAGTACCC GCCTTGTCAA CTGGTCCTGT AGTCTCAACT CTGCCATTTC TGACATTGAG 
GTAGATAAGA AGGAACTGTC GGGCCGAACC TTGCTTTCTG TCCCAGGCTA TCAGGAGAAG 
GTGGAATTTG GGGTCATTGT TTCTTTTGCC TACAAGATAC AGGATTCAGA GAGCAATGAG 
GAGGTGGTCG TAGCAACTAC TCGTATTGAG ACCATGTTGG GAGATGTAGC TGTGGCTGTG 
CACCCCAATG ACCCTAGATA CCAGCACCTT CAGGGAAAGA GTGTTATACA CCCATTCCTT 
GCTCGAAGCC TTCCTATCAT CTTTGATGAA TTTGTGGACA TGGAGTTTGG CACTGGTGCT 
GTAAAAATTA CACCAGCCCA TGATCAGAAT GACTATGAGG TTGGGCAAAG ACACAAGCTA 
GAAGCAGTGA GCATCATAGA CCACCAAGGC AACCTTATCA ATGTTCCGCC TCCCTTTCTG 
GGCCTTCCTC GGTTTGAAGC CAGGAAAGCA GTGTTGGCAG CCCTAAAAGA CAAGGGACTA 
TTCCGGGAAG TGAAGGACAA TCCAATGGTG GTACCTTTGT GCAACCGCTC CAAAGATGTC 
GTTGAACCCC TCCTTAAGCC CCAGTGGTAT GTGAGGTGTG GTGAAATGGC TCAGGCAGCC 
AGTGCAGCAG TGACTCGGGG TGACCTTAAG ATATTACCTG AGATTCATCA GAAAATATGG 
CACAATTGGA TGGACAACAT CAGAGACTGG TGCATCTCCC GGCAGCTGTG GTGGGGTCAC 
CGAATCCCTG CCTACTTTGT CACTGTCAAT GACCCTAAAG TGCCCCCTGG GGAGGATCCT 
GATGGTCGGT ATTGGGTGAG TGGCCGAAGT GAAGAGGAGG CGAGGGAGAA GGCAGCAAAG 
AAGTTTGGAG TGTCCACAGA CAAGATTAGC CTCTCACAGG ATGAAGATGT TTTAGACACA 
TGGTTTTCCT CTGGCATTTT TCCCTTTTCC ATCTTTGGAT GGCCCAACCA GACAGAGGAC 
TTAAGTATAT TCTACCCAGG GACCTTGTTG GAGACAGGAC ATGACATTTT ATTCTTCTGG 
GTAGCCCGCA TGGTGATGCT GGGTCTGAAG CTAACAGGAA AACTGCCCTT CAAAGAGGTT 
TATCTCCATG CCCTCGTCAG AGATGCCCAT GGCCGGAAAA TGAGCAAATC ACTTGGTAAT 
GTCATTGACC CCCTGGATGT CATCAGTGGA CTCTCCCTGC AGGGACTGCA TGATCAGCTA 
CTGAACAGCA ACCTTGACCC CAGTGAAATG GAGAAAGCCA AGGAGGGGCA GAAGGCAGAT 
TTCCCCAATG GAATCCCTGA ATGTGGTACA GATGCTCTCA GATTTGGACT TTGTGCTTAC 
ACTTCTCAGG GCCGGGACAT TAACCTGGAT GTGAACCGAA TCCTTGGGTA TCGACATTTC 
TGTAATAAGC TCTGGAATGC CACCAAGTTT GCCCTTAGGG CATTGGGAGA TGGATTTGTA 
CCCTCACCTA CTCCCCAAGC TAGCAATCAG GAGAGCTTGG CTGATCGTTG GATTAGGAGC 
CGCCTGAGTG AAGCTGTTGC GCTGAGCGAC CAGGGTTTCC ATGCCTACGA TTTCCCTACT 
GTCACCACTG CCCAGTATAG CTTTTGGCTC TATGACCTCT GCAATGTCTA CCTGGAGTGC 
TTGAAACCTG TGTTGTCTGG GACAGACCAA GCTGCAGCAG ATACTGCCCG GCAGACCCTC 
TATACTTGCC TGGATGTGGG CCTAAGACTA CTCTCACCCT TCATGCCTTT TGTGACTGAG 
GAACTGTATC AGCGATTGCC GCGGCGGGGG CCCCAGATGC CACCCAGTCT TTGTGTTACC 
CCCTACCCAG AGCCCACTGA GTTCTCCTGG AAGGATCCCG AGGCTGAGGC TGCTTTTGAG 
CTTGCACTTA GTATTATCCG GGCTGTGCGT TCTCTCCGAG CTGATTATAA CCTCACCCGA 
AGCCAACCTG AATGTTTTCT GGAAGTGGTG GATGAGGCAA CAGGAGTACA GGCTTCTACA 
GTGTCAGGCT ATGTGCAGGC TCTCTCCAAC ACTGGAGCTG TGACTATTCT GCTCCCTGGG 
TCCCCTGCTC CACAGGGTTG TGCTGTGGGC CTGGCCTCTG ACCGATGCTC TGTCCACCTG 
CAGCTTCAGG GGCTGGTGGA TCCTTCCCGG GAGCTGGCAA AACTCCGGGC CAAACAAGGG 
GAAGCTGAGC ATCAGGCCCA AAGGCTTCGG GAGCGTCGGG CTGTTCCTGA TTATACCACC 
AAGGTTCCTT TCCAGGTCCA GGAGTCTGAG GAAGCTAAGC TCCAACAGAC AGAGGCAGAG 
CTGAAGAAGG TAGATGAAGC TATTGCCCTG TTTGAGAAGA TGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS ( XM_023502899.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023358667.1 Sarcophilus harrisii valyl-tRNA synthetase (VARS), mRNA.

Price & Availability↑

CloneID	OSa154727
Clone ID Related Accession (Same CDS sequence)	XM_023502899.1
Accession Version	XM_023502899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838884.1) and transcript sequence (GEDN01116408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 164 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGGGGGTC CCGGAGGCAG CCGAGGAGCC ACCCTTGTCC AGCAGTGGGT CAGCTATGCG 
GATGGAGAGC TCGTGCCCGC TGGCTGTGGA GCCGCGCTGC CCGCTTTAGG GCTTCGGAAC 
CCTAATCACG ACCCTCAGGC AGCCCTGGGA GCCCTGGGAC GGGCTCTGGG CCCATTGGAG 
GAGAGACTGA GGTTGCATAC CTATTTGGTA GGAGAAGCCC CCACTTTGGC CGACCTGGCA 
GCTGTTACAG CCCTACTGCT GCCCTTCCGC TATGTTCTAG ACCCTCCTGC TCGCTGGGCT 
TGGGGTAACG TAAGCCGCTG GTTCATGACA TGTGTCCAGC AACCAGAATT TCGTGCTGTG 
TTAGGGGATG TGTCCCTGTG CCCAGGAGTG CGGTCTCTCC CACAGCAACC TGGCTCTGAG 
GTTTCTGGGT CCTCCAAGAC AGCAGCCCAG TTGAAAAAAG AAGCAAAAAA GCGGGAAAAG 
CTGGAGAAGT TTCAACAGAA GCAAAGGAAC CAATTGCATC AGCCACCTCC TGGAGAGAAG 
AAGCCAAAAA TTGAGAAGAA GGAGAAACGG GACCCTGGGG TCATAACATA TGATATCCCC 
ACCCCACCAG GGGAGAAGAA AGATGTAAGT GTGTCCATGC CTGACTCCTA TAGTCCCCAG 
TATGTTGAAG CTGCTTGGTA TTCATGGTGG GAGAGCCAAG GATTCTTCAA ACCAGAGTAT 
GGGCGTGCCA GTGTGTCAGA GCCTAATCCT CGAGGTGTCT TCATGATGTG CATCCCGCCT 
CCCAATGTGA CAGGGTCCCT GCACCTTGGC CATGCCCTCA CCAATGCCAT CCAGGATTCA 
CTGACCCGCT GGCACCGAAT GAGAGGAGAA ACAACCCTGT GGAACCCAGG CTGTGATCAT 
GCTGGTATTG CCACACAGGT AGTTGTGGAA AAGAAACTAT GGAGGGAACA TGGGCTAAGC 
CGCCACCAGC TTGGTCGAGA GGCCTTCCTA CAAGAGGTCT GGAAATGGAA GAATGAGAAG 
GGAGACCGGA TTTACCATCA GCTAAGGAAG CTTGGTGGCT CCTTGGATTG GGATCGAGCT 
TGTTTCACCA TGGATCCTAA ACTCTCAGCA GCTGTGACAG AAGCTTTTGT TCGCCTCCAT 
AATGATGGGG TCATTTACCG GAGTACCCGC CTTGTCAACT GGTCCTGTAG TCTCAACTCT 
GCCATTTCTG ACATTGAGGT AGATAAGAAG GAACTGTCGG GCCGAACCTT GCTTTCTGTC 
CCAGGCTATC AGGAGAAGGT GGAATTTGGG GTCATTGTTT CTTTTGCCTA CAAGATACAG 
GATTCAGAGA GCAATGAGGA GGTGGTCGTA GCAACTACTC GTATTGAGAC CATGTTGGGA 
GATGTAGCTG TGGCTGTGCA CCCCAATGAC CCTAGATACC AGCACCTTCA GGGAAAGAGT 
GTTATACACC CATTCCTTGC TCGAAGCCTT CCTATCATCT TTGATGAATT TGTGGACATG 
GAGTTTGGCA CTGGTGCTGT AAAAATTACA CCAGCCCATG ATCAGAATGA CTATGAGGTT 
GGGCAAAGAC ACAAGCTAGA AGCAGTGAGC ATCATAGACC ACCAAGGCAA CCTTATCAAT 
GTTCCGCCTC CCTTTCTGGG CCTTCCTCGG TTTGAAGCCA GGAAAGCAGT GTTGGCAGCC 
CTAAAAGACA AGGGACTATT CCGGGAAGTG AAGGACAATC CAATGGTGGT ACCTTTGTGC 
AACCGCTCCA AAGATGTCGT TGAACCCCTC CTTAAGCCCC AGTGGTATGT GAGGTGTGGT 
GAAATGGCTC AGGCAGCCAG TGCAGCAGTG ACTCGGGGTG ACCTTAAGAT ATTACCTGAG 
ATTCATCAGA AAATATGGCA CAATTGGATG GACAACATCA GAGACTGGTG CATCTCCCGG 
CAGCTGTGGT GGGGTCACCG AATCCCTGCC TACTTTGTCA CTGTCAATGA CCCTAAAGTG 
CCCCCTGGGG AGGATCCTGA TGGTCGGTAT TGGGTGAGTG GCCGAAGTGA AGAGGAGGCG 
AGGGAGAAGG CAGCAAAGAA GTTTGGAGTG TCCACAGACA AGATTAGCCT CTCACAGGAT 
GAAGATGTTT TAGACACATG GTTTTCCTCT GGCATTTTTC CCTTTTCCAT CTTTGGATGG 
CCCAACCAGA CAGAGGACTT AAGTATATTC TACCCAGGGA CCTTGTTGGA GACAGGACAT 
GACATTTTAT TCTTCTGGGT AGCCCGCATG GTGATGCTGG GTCTGAAGCT AACAGGAAAA 
CTGCCCTTCA AAGAGGTTTA TCTCCATGCC CTCGTCAGAG ATGCCCATGG CCGGAAAATG 
AGCAAATCAC TTGGTAATGT CATTGACCCC CTGGATGTCA TCAGTGGACT CTCCCTGCAG 
GGACTGCATG ATCAGCTACT GAACAGCAAC CTTGACCCCA GTGAAATGGA GAAAGCCAAG 
GAGGGGCAGA AGGCAGATTT CCCCAATGGA ATCCCTGAAT GTGGTACAGA TGCTCTCAGA 
TTTGGACTTT GTGCTTACAC TTCTCAGGGC CGGGACATTA ACCTGGATGT GAACCGAATC 
CTTGGGTATC GACATTTCTG TAATAAGCTC TGGAATGCCA CCAAGTTTGC CCTTAGGGCA 
TTGGGAGATG GATTTGTACC CTCACCTACT CCCCAAGCTA GCAATCAGGA GAGCTTGGCT 
GATCGTTGGA TTAGGAGCCG CCTGAGTGAA GCTGTTGCGC TGAGCGACCA GGGTTTCCAT 
GCCTACGATT TCCCTACTGT CACCACTGCC CAGTATAGCT TTTGGCTCTA TGACCTCTGC 
AATGTCTACC TGGAGTGCTT GAAACCTGTG TTGTCTGGGA CAGACCAAGC TGCAGCAGAT 
ACTGCCCGGC AGACCCTCTA TACTTGCCTG GATGTGGGCC TAAGACTACT CTCACCCTTC 
ATGCCTTTTG TGACTGAGGA ACTGTATCAG CGATTGCCGC GGCGGGGGCC CCAGATGCCA 
CCCAGTCTTT GTGTTACCCC CTACCCAGAG CCCACTGAGT TCTCCTGGAA GGATCCCGAG 
GCTGAGGCTG CTTTTGAGCT TGCACTTAGT ATTATCCGGG CTGTGCGTTC TCTCCGAGCT 
GATTATAACC TCACCCGAAG CCAACCTGAA TGTTTTCTGG AAGTGGTGGA TGAGGCAACA 
GGAGTACAGG CTTCTACAGT GTCAGGCTAT GTGCAGGCTC TCTCCAACAC TGGAGCTGTG 
ACTATTCTGC TCCCTGGGTC CCCTGCTCCA CAGGGTTGTG CTGTGGGCCT GGCCTCTGAC 
CGATGCTCTG TCCACCTGCA GCTTCAGGGG CTGGTGGATC CTTCCCGGGA GCTGGCAAAA 
CTCCGGGCCA AACAAGGGGA AGCTGAGCAT CAGGCCCAAA GGCTTCGGGA GCGTCGGGCT 
GTTCCTGATT ATACCACCAA GGTTCCTTTC CAGGTCCAGG AGTCTGAGGA AGCTAAGCTC 
CAACAGACAG AGGCAGAGCT GAAGAAGGTA GATGAAGCTA TTGCCCTGTT TGAGAAGATG 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023358667.1
CDS	27..3572
Translation	MGGPGGSRGATLVQQWVSYADGELVPAGCGAALPALGLRNPNHDPQAALGALGRALGPLEERLRLHTYLVGEAPTLADLAAVTALLLPFRYVLDPPARWAWGNVSRWFMTCVQQPEFRAVLGDVSLCPGVRSLPQQPGSEVSGSSKTAAQLKKEAKKREKLEKFQQKQRNQLHQPPPGEKKPKIEKKEKRDPGVITYDIPTPPGEKKDVSVSMPDSYSPQYVEAAWYSWWESQGFFKPEYGRASVSEPNPRGVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREHGLSRHQLGREAFLQEVWKWKNEKGDRIYHQLRKLGGSLDWDRACFTMDPKLSAAVTEAFVRLHNDGVIYRSTRLVNWSCSLNSAISDIEVDKKELSGRTLLSVPGYQEKVEFGVIVSFAYKIQDSESNEEVVVATTRIETMLGDVAVAVHPNDPRYQHLQGKSVIHPFLARSLPIIFDEFVDMEFGTGAVKITPAHDQNDYEVGQRHKLEAVSIIDHQGNLINVPPPFLGLPRFEARKAVLAALKDKGLFREVKDNPMVVPLCNRSKDVVEPLLKPQWYVRCGEMAQAASAAVTRGDLKILPEIHQKIWHNWMDNIRDWCISRQLWWGHRIPAYFVTVNDPKVPPGEDPDGRYWVSGRSEEEAREKAAKKFGVSTDKISLSQDEDVLDTWFSSGIFPFSIFGWPNQTEDLSIFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFKEVYLHALVRDAHGRKMSKSLGNVIDPLDVISGLSLQGLHDQLLNSNLDPSEMEKAKEGQKADFPNGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRALGDGFVPSPTPQASNQESLADRWIRSRLSEAVALSDQGFHAYDFPTVTTAQYSFWLYDLCNVYLECLKPVLSGTDQAAADTARQTLYTCLDVGLRLLSPFMPFVTEELYQRLPRRGPQMPPSLCVTPYPEPTEFSWKDPEAEAAFELALSIIRAVRSLRADYNLTRSQPECFLEVVDEATGVQASTVSGYVQALSNTGAVTILLPGSPAPQGCAVGLASDRCSVHLQLQGLVDPSRELAKLRAKQGEAEHQAQRLRERRAVPDYTTKVPFQVQESEEAKLQQTEAELKKVDEAIALFEKML

Target ORF information:

RefSeq Version	XM_023502899.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii valyl-tRNA synthetase (VARS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023502899.1

ORF Insert Sequence:

ATGGGGGGTC CCGGAGGCAG CCGAGGAGCC ACCCTTGTCC AGCAGTGGGT CAGCTATGCG 
GATGGAGAGC TCGTGCCCGC TGGCTGTGGA GCCGCGCTGC CCGCTTTAGG GCTTCGGAAC 
CCTAATCACG ACCCTCAGGC AGCCCTGGGA GCCCTGGGAC GGGCTCTGGG CCCATTGGAG 
GAGAGACTGA GGTTGCATAC CTATTTGGTA GGAGAAGCCC CCACTTTGGC CGACCTGGCA 
GCTGTTACAG CCCTACTGCT GCCCTTCCGC TATGTTCTAG ACCCTCCTGC TCGCTGGGCT 
TGGGGTAACG TAAGCCGCTG GTTCATGACA TGTGTCCAGC AACCAGAATT TCGTGCTGTG 
TTAGGGGATG TGTCCCTGTG CCCAGGAGTG CGGTCTCTCC CACAGCAACC TGGCTCTGAG 
GTTTCTGGGT CCTCCAAGAC AGCAGCCCAG TTGAAAAAAG AAGCAAAAAA GCGGGAAAAG 
CTGGAGAAGT TTCAACAGAA GCAAAGGAAC CAATTGCATC AGCCACCTCC TGGAGAGAAG 
AAGCCAAAAA TTGAGAAGAA GGAGAAACGG GACCCTGGGG TCATAACATA TGATATCCCC 
ACCCCACCAG GGGAGAAGAA AGATGTAAGT GTGTCCATGC CTGACTCCTA TAGTCCCCAG 
TATGTTGAAG CTGCTTGGTA TTCATGGTGG GAGAGCCAAG GATTCTTCAA ACCAGAGTAT 
GGGCGTGCCA GTGTGTCAGA GCCTAATCCT CGAGGTGTCT TCATGATGTG CATCCCGCCT 
CCCAATGTGA CAGGGTCCCT GCACCTTGGC CATGCCCTCA CCAATGCCAT CCAGGATTCA 
CTGACCCGCT GGCACCGAAT GAGAGGAGAA ACAACCCTGT GGAACCCAGG CTGTGATCAT 
GCTGGTATTG CCACACAGGT AGTTGTGGAA AAGAAACTAT GGAGGGAACA TGGGCTAAGC 
CGCCACCAGC TTGGTCGAGA GGCCTTCCTA CAAGAGGTCT GGAAATGGAA GAATGAGAAG 
GGAGACCGGA TTTACCATCA GCTAAGGAAG CTTGGTGGCT CCTTGGATTG GGATCGAGCT 
TGTTTCACCA TGGATCCTAA ACTCTCAGCA GCTGTGACAG AAGCTTTTGT TCGCCTCCAT 
AATGATGGGG TCATTTACCG GAGTACCCGC CTTGTCAACT GGTCCTGTAG TCTCAACTCT 
GCCATTTCTG ACATTGAGGT AGATAAGAAG GAACTGTCGG GCCGAACCTT GCTTTCTGTC 
CCAGGCTATC AGGAGAAGGT GGAATTTGGG GTCATTGTTT CTTTTGCCTA CAAGATACAG 
GATTCAGAGA GCAATGAGGA GGTGGTCGTA GCAACTACTC GTATTGAGAC CATGTTGGGA 
GATGTAGCTG TGGCTGTGCA CCCCAATGAC CCTAGATACC AGCACCTTCA GGGAAAGAGT 
GTTATACACC CATTCCTTGC TCGAAGCCTT CCTATCATCT TTGATGAATT TGTGGACATG 
GAGTTTGGCA CTGGTGCTGT AAAAATTACA CCAGCCCATG ATCAGAATGA CTATGAGGTT 
GGGCAAAGAC ACAAGCTAGA AGCAGTGAGC ATCATAGACC ACCAAGGCAA CCTTATCAAT 
GTTCCGCCTC CCTTTCTGGG CCTTCCTCGG TTTGAAGCCA GGAAAGCAGT GTTGGCAGCC 
CTAAAAGACA AGGGACTATT CCGGGAAGTG AAGGACAATC CAATGGTGGT ACCTTTGTGC 
AACCGCTCCA AAGATGTCGT TGAACCCCTC CTTAAGCCCC AGTGGTATGT GAGGTGTGGT 
GAAATGGCTC AGGCAGCCAG TGCAGCAGTG ACTCGGGGTG ACCTTAAGAT ATTACCTGAG 
ATTCATCAGA AAATATGGCA CAATTGGATG GACAACATCA GAGACTGGTG CATCTCCCGG 
CAGCTGTGGT GGGGTCACCG AATCCCTGCC TACTTTGTCA CTGTCAATGA CCCTAAAGTG 
CCCCCTGGGG AGGATCCTGA TGGTCGGTAT TGGGTGAGTG GCCGAAGTGA AGAGGAGGCG 
AGGGAGAAGG CAGCAAAGAA GTTTGGAGTG TCCACAGACA AGATTAGCCT CTCACAGGAT 
GAAGATGTTT TAGACACATG GTTTTCCTCT GGCATTTTTC CCTTTTCCAT CTTTGGATGG 
CCCAACCAGA CAGAGGACTT AAGTATATTC TACCCAGGGA CCTTGTTGGA GACAGGACAT 
GACATTTTAT TCTTCTGGGT AGCCCGCATG GTGATGCTGG GTCTGAAGCT AACAGGAAAA 
CTGCCCTTCA AAGAGGTTTA TCTCCATGCC CTCGTCAGAG ATGCCCATGG CCGGAAAATG 
AGCAAATCAC TTGGTAATGT CATTGACCCC CTGGATGTCA TCAGTGGACT CTCCCTGCAG 
GGACTGCATG ATCAGCTACT GAACAGCAAC CTTGACCCCA GTGAAATGGA GAAAGCCAAG 
GAGGGGCAGA AGGCAGATTT CCCCAATGGA ATCCCTGAAT GTGGTACAGA TGCTCTCAGA 
TTTGGACTTT GTGCTTACAC TTCTCAGGGC CGGGACATTA ACCTGGATGT GAACCGAATC 
CTTGGGTATC GACATTTCTG TAATAAGCTC TGGAATGCCA CCAAGTTTGC CCTTAGGGCA 
TTGGGAGATG GATTTGTACC CTCACCTACT CCCCAAGCTA GCAATCAGGA GAGCTTGGCT 
GATCGTTGGA TTAGGAGCCG CCTGAGTGAA GCTGTTGCGC TGAGCGACCA GGGTTTCCAT 
GCCTACGATT TCCCTACTGT CACCACTGCC CAGTATAGCT TTTGGCTCTA TGACCTCTGC 
AATGTCTACC TGGAGTGCTT GAAACCTGTG TTGTCTGGGA CAGACCAAGC TGCAGCAGAT 
ACTGCCCGGC AGACCCTCTA TACTTGCCTG GATGTGGGCC TAAGACTACT CTCACCCTTC 
ATGCCTTTTG TGACTGAGGA ACTGTATCAG CGATTGCCGC GGCGGGGGCC CCAGATGCCA 
CCCAGTCTTT GTGTTACCCC CTACCCAGAG CCCACTGAGT TCTCCTGGAA GGATCCCGAG 
GCTGAGGCTG CTTTTGAGCT TGCACTTAGT ATTATCCGGG CTGTGCGTTC TCTCCGAGCT 
GATTATAACC TCACCCGAAG CCAACCTGAA TGTTTTCTGG AAGTGGTGGA TGAGGCAACA 
GGAGTACAGG CTTCTACAGT GTCAGGCTAT GTGCAGGCTC TCTCCAACAC TGGAGCTGTG 
ACTATTCTGC TCCCTGGGTC CCCTGCTCCA CAGGGTTGTG CTGTGGGCCT GGCCTCTGAC 
CGATGCTCTG TCCACCTGCA GCTTCAGGGG CTGGTGGATC CTTCCCGGGA GCTGGCAAAA 
CTCCGGGCCA AACAAGGGGA AGCTGAGCAT CAGGCCCAAA GGCTTCGGGA GCGTCGGGCT 
GTTCCTGATT ATACCACCAA GGTTCCTTTC CAGGTCCAGG AGTCTGAGGA AGCTAAGCTC 
CAACAGACAG AGGCAGAGCT GAAGAAGGTA GATGAAGCTA TTGCCCTGTT TGAGAAGATG 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS ( XM_031965157.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031821017.1 Sarcophilus harrisii valyl-tRNA synthetase 1 (VARS1), transcript variant X1, mRNA.

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CloneID	OSa233182
Clone ID Related Accession (Same CDS sequence)	XM_031965157.1 , XM_031965158.1 , XM_031965159.1
Accession Version	XM_031965157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCC TGTATGTGTC TCCTCACCCG GACGCCTTCC CCAGCCTTCG CGCCCTGATT 
GCTGCCCGAT ACGGGGAGTC TGGAACTGGC CCAGGGTGGG GAGGGCCTCC TCCCCGCGTC 
TGCCTCCAGG CACCCCCTAC CAGCGGCACT TCCATCCCCC CGCCTCGGCT GCCGGTTCTC 
GAGCAGGGAC CAGGGGGCTT AAGGGTATGG GGCGCGGCCG CTGTCGCGCA GCTCCTGTGG 
CCCGCGGGAA TGGGGGGTCC CGGAGGCAGC CGAGGAGCCA CCCTTGTCCA GCAGTGGGTC 
AGCTATGCGG ATGGAGAGCT CGTGCCCGCT GGCTGTGGAG CCGCGCTGCC CGCTTTAGGG 
CTTCGGAACC CTAATCACGA CCCTCAGGCA GCCCTGGGAG CCCTGGGACG GGCTCTGGGC 
CCATTGGAGG AGAGACTGAG GTTGCATACC TATTTGGTAG GAGAAGCCCC CACTTTGGCC 
GACCTGGCAG CTGTTACAGC CCTACTGCTG CCCTTCCGCT ATGTTCTAGA CCCTCCTGCT 
CGCTGGGCTT GGGGTAACGT AAGCCGCTGG TTCATGACAT GTGTCCAGCA ACCAGAATTT 
CGTGCTGTGT TAGGGGATGT GTCCCTGTGC CCAGGAGTGC GGTCTCTCCC ACAGCAACCT 
GGCTCTGAGG TTTCTGGGTC CTCCAAGACA GCAGCCCAGT TGAAAAAAGA AGCAAAAAAG 
CGGGAAAAGC TGGAGAAGTT TCAACAGAAG CAAAGGAACC AATTGCATCA GCCACCTCCT 
GGAGAGAAGA AGCCAAAAAT TGAGAAGAAG GAGAAACGGG ACCCTGGGGT CATAACATAT 
GATATCCCCA CCCCACCAGG GGAGAAGAAA GATGTAAGTG TGTCCATGCC TGACTCCTAT 
AGTCCCCAGT ATGTTGAAGC TGCTTGGTAT TCATGGTGGG AGAGCCAAGG ATTCTTCAAA 
CCAGAGTATG GGCGTGCCAG TGTGTCAGAG CCTAATCCTC GAGGTGTCTT CATGATGTGC 
ATCCCGCCTC CCAATGTGAC AGGGTCCCTG CACCTTGGCC ATGCCCTCAC CAATGCCATC 
CAGGATTCAC TGACCCGCTG GCACCGAATG AGAGGAGAAA CAACCCTGTG GAACCCAGGC 
TGTGATCATG CTGGTATTGC CACACAGGTA GTTGTGGAAA AGAAACTATG GAGGGAACAT 
GGGCTAAGCC GCCACCAGCT TGGTCGAGAG GCCTTCCTAC AAGAGGTCTG GAAATGGAAG 
AATGAGAAGG GAGACCGGAT TTACCATCAG CTAAGGAAGC TTGGTGGCTC CTTGGATTGG 
GATCGAGCTT GTTTCACCAT GGATCCTAAA CTCTCAGCAG CTGTGACAGA AGCTTTTGTT 
CGCCTCCATA ATGATGGGGT CATTTACCGG AGTACCCGCC TTGTCAACTG GTCCTGTAGT 
CTCAACTCTG CCATTTCTGA CATTGAGGTA GATAAGAAGG AACTGTCGGG CCGAACCTTG 
CTTTCTGTCC CAGGCTATCA GGAGAAGGTG GAATTTGGGG TCATTGTTTC TTTTGCCTAC 
AAGATACAGG ATTCAGAGAG CAATGAGGAG GTGGTCGTAG CAACTACTCG TATTGAGACC 
ATGTTGGGAG ATGTAGCTGT GGCTGTGCAC CCCAATGACC CTAGATACCA GCACCTTCAG 
GGAAAGAGTG TTATACACCC ATTCCTTGCT CGAAGCCTTC CTATCATCTT TGATGAATTT 
GTGGACATGG AGTTTGGCAC TGGTGCTGTA AAAATTACAC CAGCCCATGA TCAGAATGAC 
TATGAGGTTG GGCAAAGACA CAAGCTAGAA GCAGTGAGCA TCATAGACCA CCAAGGCAAC 
CTTATCAATG TTCCGCCTCC CTTTCTGGGC CTTCCTCGGT TTGAAGCCAG GAAAGCAGTG 
TTGGCAGCCC TAAAAGACAA GGGACTATTC CGGGAAGTGA AGGACAATCC AATGGTGGTA 
CCTTTGTGCA ACCGCTCCAA AGATGTCGTT GAACCCCTCC TTAAGCCCCA GTGGTATGTG 
AGGTGTGGTG AAATGGCTCA GGCAGCCAGT GCAGCAGTGA CTCGGGGTGA CCTTAAGATA 
TTACCTGAGA TTCATCAGAA AATATGGCAC AATTGGATGG ACAACATCAG AGACTGGTGC 
ATCTCCCGGC AGCTGTGGTG GGGTCACCGA ATCCCTGCCT ACTTTGTCAC TGTCAATGAC 
CCTAAAGTGC CCCCTGGGGA GGATCCTGAT GGTCGGTATT GGGTGAGTGG CCGAAGTGAA 
GAGGAGGCGA GGGAGAAGGC AGCAAAGAAG TTTGGAGTGT CCACAGACAA GATTAGCCTC 
TCACAGGATG AAGATGTTTT AGACACATGG TTTTCCTCTG GCATTTTTCC CTTTTCCATC 
TTTGGATGGC CCAACCAGAC AGAGGACTTA AGTATATTCT ACCCAGGGAC CTTGTTGGAG 
ACAGGACATG ACATTTTATT CTTCTGGGTA GCCCGCATGG TGATGCTGGG TCTGAAGCTA 
ACAGGAAAAC TGCCCTTCAA AGAGGTTTAT CTCCATGCCC TCGTCAGAGA TGCCCATGGC 
CGGAAAATGA GCAAATCACT TGGTAATGTC ATTGACCCCC TGGATGTCAT CAGTGGACTC 
TCCCTGCAGG GACTGCATGA TCAGCTACTG AACAGCAACC TTGACCCCAG TGAAATGGAG 
AAAGCCAAGG AGGGGCAGAA GGCAGATTTC CCCAATGGAA TCCCTGAATG TGGTACAGAT 
GCTCTCAGAT TTGGACTTTG TGCTTACACT TCTCAGGGCC GGGACATTAA CCTGGATGTG 
AACCGAATCC TTGGGTATCG ACATTTCTGT AATAAGCTCT GGAATGCCAC CAAGTTTGCC 
CTTAGGGCAT TGGGAGATGG ATTTGTACCC TCACCTACTC CCCAAGCTAG CAATCAGGAG 
AGCTTGGCTG ATCGTTGGAT TAGGAGCCGC CTGAGTGAAG CTGTTGCGCT GAGCGACCAG 
GGTTTCCATG CCTACGATTT CCCTACTGTC ACCACTGCCC AGTATAGCTT TTGGCTCTAT 
GACCTCTGCA ATGTCTACCT GGAGTGCTTG AAACCTGTGT TGTCTGGGAC AGACCAAGCT 
GCAGCAGATA CTGCCCGGCA GACCCTCTAT ACTTGCCTGG ATGTGGGCCT AAGACTACTC 
TCACCCTTCA TGCCTTTTGT GACTGAGGAA CTGTATCAGC GATTGCCGCG GCGGGGGCCC 
CAGATGCCAC CCAGTCTTTG TGTTACCCCC TACCCAGAGC CCACTGAGTT CTCCTGGAAG 
GATCCCGAGG CTGAGGCTGC TTTTGAGCTT GCACTTAGTA TTATCCGGGC TGTGCGTTCT 
CTCCGAGCTG ATTATAACCT CACCCGAAGC CAACCTGAAT GTTTTCTGGA AGTGGTGGAT 
GAGGCAACAG GAGTACAGGC TTCTACAGTG TCAGGCTATG TGCAGGCTCT CTCCAACACT 
GGAGCTGTGA CTATTCTGCT CCCTGGGTCC CCTGCTCCAC AGGGTTGTGC TGTGGGCCTG 
GCCTCTGACC GATGCTCTGT CCACCTGCAG CTTCAGGGGC TGGTGGATCC TTCCCGGGAG 
CTGGCAAAAC TCCGGGCCAA ACAAGGGGAA GCTGAGCATC AGGCCCAAAG GCTTCGGGAG 
CGTCGGGCTG TTCCTGATTA TACCACCAAG GTTCCTTTCC AGGTCCAGGA GTCTGAGGAA 
GCTAAGCTCC AACAGACAGA GGCAGAGCTG AAGAAGGTAG ATGAAGCTAT TGCCCTGTTT 
GAGAAGATGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031821017.1
CDS	331..4125
Translation	MSILYVSPHPDAFPSLRALIAARYGESGTGPGWGGPPPRVCLQAPPTSGTSIPPPRLPVLEQGPGGLRVWGAAAVAQLLWPAGMGGPGGSRGATLVQQWVSYADGELVPAGCGAALPALGLRNPNHDPQAALGALGRALGPLEERLRLHTYLVGEAPTLADLAAVTALLLPFRYVLDPPARWAWGNVSRWFMTCVQQPEFRAVLGDVSLCPGVRSLPQQPGSEVSGSSKTAAQLKKEAKKREKLEKFQQKQRNQLHQPPPGEKKPKIEKKEKRDPGVITYDIPTPPGEKKDVSVSMPDSYSPQYVEAAWYSWWESQGFFKPEYGRASVSEPNPRGVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREHGLSRHQLGREAFLQEVWKWKNEKGDRIYHQLRKLGGSLDWDRACFTMDPKLSAAVTEAFVRLHNDGVIYRSTRLVNWSCSLNSAISDIEVDKKELSGRTLLSVPGYQEKVEFGVIVSFAYKIQDSESNEEVVVATTRIETMLGDVAVAVHPNDPRYQHLQGKSVIHPFLARSLPIIFDEFVDMEFGTGAVKITPAHDQNDYEVGQRHKLEAVSIIDHQGNLINVPPPFLGLPRFEARKAVLAALKDKGLFREVKDNPMVVPLCNRSKDVVEPLLKPQWYVRCGEMAQAASAAVTRGDLKILPEIHQKIWHNWMDNIRDWCISRQLWWGHRIPAYFVTVNDPKVPPGEDPDGRYWVSGRSEEEAREKAAKKFGVSTDKISLSQDEDVLDTWFSSGIFPFSIFGWPNQTEDLSIFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFKEVYLHALVRDAHGRKMSKSLGNVIDPLDVISGLSLQGLHDQLLNSNLDPSEMEKAKEGQKADFPNGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRALGDGFVPSPTPQASNQESLADRWIRSRLSEAVALSDQGFHAYDFPTVTTAQYSFWLYDLCNVYLECLKPVLSGTDQAAADTARQTLYTCLDVGLRLLSPFMPFVTEELYQRLPRRGPQMPPSLCVTPYPEPTEFSWKDPEAEAAFELALSIIRAVRSLRADYNLTRSQPECFLEVVDEATGVQASTVSGYVQALSNTGAVTILLPGSPAPQGCAVGLASDRCSVHLQLQGLVDPSRELAKLRAKQGEAEHQAQRLRERRAVPDYTTKVPFQVQESEEAKLQQTEAELKKVDEAIALFEKML

Target ORF information:

RefSeq Version	XM_031965157.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii valyl-tRNA synthetase 1 (VARS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031965157.1

ORF Insert Sequence:

ATGTCCATCC TGTATGTGTC TCCTCACCCG GACGCCTTCC CCAGCCTTCG CGCCCTGATT 
GCTGCCCGAT ACGGGGAGTC TGGAACTGGC CCAGGGTGGG GAGGGCCTCC TCCCCGCGTC 
TGCCTCCAGG CACCCCCTAC CAGCGGCACT TCCATCCCCC CGCCTCGGCT GCCGGTTCTC 
GAGCAGGGAC CAGGGGGCTT AAGGGTATGG GGCGCGGCCG CTGTCGCGCA GCTCCTGTGG 
CCCGCGGGAA TGGGGGGTCC CGGAGGCAGC CGAGGAGCCA CCCTTGTCCA GCAGTGGGTC 
AGCTATGCGG ATGGAGAGCT CGTGCCCGCT GGCTGTGGAG CCGCGCTGCC CGCTTTAGGG 
CTTCGGAACC CTAATCACGA CCCTCAGGCA GCCCTGGGAG CCCTGGGACG GGCTCTGGGC 
CCATTGGAGG AGAGACTGAG GTTGCATACC TATTTGGTAG GAGAAGCCCC CACTTTGGCC 
GACCTGGCAG CTGTTACAGC CCTACTGCTG CCCTTCCGCT ATGTTCTAGA CCCTCCTGCT 
CGCTGGGCTT GGGGTAACGT AAGCCGCTGG TTCATGACAT GTGTCCAGCA ACCAGAATTT 
CGTGCTGTGT TAGGGGATGT GTCCCTGTGC CCAGGAGTGC GGTCTCTCCC ACAGCAACCT 
GGCTCTGAGG TTTCTGGGTC CTCCAAGACA GCAGCCCAGT TGAAAAAAGA AGCAAAAAAG 
CGGGAAAAGC TGGAGAAGTT TCAACAGAAG CAAAGGAACC AATTGCATCA GCCACCTCCT 
GGAGAGAAGA AGCCAAAAAT TGAGAAGAAG GAGAAACGGG ACCCTGGGGT CATAACATAT 
GATATCCCCA CCCCACCAGG GGAGAAGAAA GATGTAAGTG TGTCCATGCC TGACTCCTAT 
AGTCCCCAGT ATGTTGAAGC TGCTTGGTAT TCATGGTGGG AGAGCCAAGG ATTCTTCAAA 
CCAGAGTATG GGCGTGCCAG TGTGTCAGAG CCTAATCCTC GAGGTGTCTT CATGATGTGC 
ATCCCGCCTC CCAATGTGAC AGGGTCCCTG CACCTTGGCC ATGCCCTCAC CAATGCCATC 
CAGGATTCAC TGACCCGCTG GCACCGAATG AGAGGAGAAA CAACCCTGTG GAACCCAGGC 
TGTGATCATG CTGGTATTGC CACACAGGTA GTTGTGGAAA AGAAACTATG GAGGGAACAT 
GGGCTAAGCC GCCACCAGCT TGGTCGAGAG GCCTTCCTAC AAGAGGTCTG GAAATGGAAG 
AATGAGAAGG GAGACCGGAT TTACCATCAG CTAAGGAAGC TTGGTGGCTC CTTGGATTGG 
GATCGAGCTT GTTTCACCAT GGATCCTAAA CTCTCAGCAG CTGTGACAGA AGCTTTTGTT 
CGCCTCCATA ATGATGGGGT CATTTACCGG AGTACCCGCC TTGTCAACTG GTCCTGTAGT 
CTCAACTCTG CCATTTCTGA CATTGAGGTA GATAAGAAGG AACTGTCGGG CCGAACCTTG 
CTTTCTGTCC CAGGCTATCA GGAGAAGGTG GAATTTGGGG TCATTGTTTC TTTTGCCTAC 
AAGATACAGG ATTCAGAGAG CAATGAGGAG GTGGTCGTAG CAACTACTCG TATTGAGACC 
ATGTTGGGAG ATGTAGCTGT GGCTGTGCAC CCCAATGACC CTAGATACCA GCACCTTCAG 
GGAAAGAGTG TTATACACCC ATTCCTTGCT CGAAGCCTTC CTATCATCTT TGATGAATTT 
GTGGACATGG AGTTTGGCAC TGGTGCTGTA AAAATTACAC CAGCCCATGA TCAGAATGAC 
TATGAGGTTG GGCAAAGACA CAAGCTAGAA GCAGTGAGCA TCATAGACCA CCAAGGCAAC 
CTTATCAATG TTCCGCCTCC CTTTCTGGGC CTTCCTCGGT TTGAAGCCAG GAAAGCAGTG 
TTGGCAGCCC TAAAAGACAA GGGACTATTC CGGGAAGTGA AGGACAATCC AATGGTGGTA 
CCTTTGTGCA ACCGCTCCAA AGATGTCGTT GAACCCCTCC TTAAGCCCCA GTGGTATGTG 
AGGTGTGGTG AAATGGCTCA GGCAGCCAGT GCAGCAGTGA CTCGGGGTGA CCTTAAGATA 
TTACCTGAGA TTCATCAGAA AATATGGCAC AATTGGATGG ACAACATCAG AGACTGGTGC 
ATCTCCCGGC AGCTGTGGTG GGGTCACCGA ATCCCTGCCT ACTTTGTCAC TGTCAATGAC 
CCTAAAGTGC CCCCTGGGGA GGATCCTGAT GGTCGGTATT GGGTGAGTGG CCGAAGTGAA 
GAGGAGGCGA GGGAGAAGGC AGCAAAGAAG TTTGGAGTGT CCACAGACAA GATTAGCCTC 
TCACAGGATG AAGATGTTTT AGACACATGG TTTTCCTCTG GCATTTTTCC CTTTTCCATC 
TTTGGATGGC CCAACCAGAC AGAGGACTTA AGTATATTCT ACCCAGGGAC CTTGTTGGAG 
ACAGGACATG ACATTTTATT CTTCTGGGTA GCCCGCATGG TGATGCTGGG TCTGAAGCTA 
ACAGGAAAAC TGCCCTTCAA AGAGGTTTAT CTCCATGCCC TCGTCAGAGA TGCCCATGGC 
CGGAAAATGA GCAAATCACT TGGTAATGTC ATTGACCCCC TGGATGTCAT CAGTGGACTC 
TCCCTGCAGG GACTGCATGA TCAGCTACTG AACAGCAACC TTGACCCCAG TGAAATGGAG 
AAAGCCAAGG AGGGGCAGAA GGCAGATTTC CCCAATGGAA TCCCTGAATG TGGTACAGAT 
GCTCTCAGAT TTGGACTTTG TGCTTACACT TCTCAGGGCC GGGACATTAA CCTGGATGTG 
AACCGAATCC TTGGGTATCG ACATTTCTGT AATAAGCTCT GGAATGCCAC CAAGTTTGCC 
CTTAGGGCAT TGGGAGATGG ATTTGTACCC TCACCTACTC CCCAAGCTAG CAATCAGGAG 
AGCTTGGCTG ATCGTTGGAT TAGGAGCCGC CTGAGTGAAG CTGTTGCGCT GAGCGACCAG 
GGTTTCCATG CCTACGATTT CCCTACTGTC ACCACTGCCC AGTATAGCTT TTGGCTCTAT 
GACCTCTGCA ATGTCTACCT GGAGTGCTTG AAACCTGTGT TGTCTGGGAC AGACCAAGCT 
GCAGCAGATA CTGCCCGGCA GACCCTCTAT ACTTGCCTGG ATGTGGGCCT AAGACTACTC 
TCACCCTTCA TGCCTTTTGT GACTGAGGAA CTGTATCAGC GATTGCCGCG GCGGGGGCCC 
CAGATGCCAC CCAGTCTTTG TGTTACCCCC TACCCAGAGC CCACTGAGTT CTCCTGGAAG 
GATCCCGAGG CTGAGGCTGC TTTTGAGCTT GCACTTAGTA TTATCCGGGC TGTGCGTTCT 
CTCCGAGCTG ATTATAACCT CACCCGAAGC CAACCTGAAT GTTTTCTGGA AGTGGTGGAT 
GAGGCAACAG GAGTACAGGC TTCTACAGTG TCAGGCTATG TGCAGGCTCT CTCCAACACT 
GGAGCTGTGA CTATTCTGCT CCCTGGGTCC CCTGCTCCAC AGGGTTGTGC TGTGGGCCTG 
GCCTCTGACC GATGCTCTGT CCACCTGCAG CTTCAGGGGC TGGTGGATCC TTCCCGGGAG 
CTGGCAAAAC TCCGGGCCAA ACAAGGGGAA GCTGAGCATC AGGCCCAAAG GCTTCGGGAG 
CGTCGGGCTG TTCCTGATTA TACCACCAAG GTTCCTTTCC AGGTCCAGGA GTCTGAGGAA 
GCTAAGCTCC AACAGACAGA GGCAGAGCTG AAGAAGGTAG ATGAAGCTAT TGCCCTGTTT 
GAGAAGATGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS ( XM_031965158.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031821018.1 Sarcophilus harrisii valyl-tRNA synthetase 1 (VARS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa233182
Clone ID Related Accession (Same CDS sequence)	XM_031965157.1 , XM_031965158.1 , XM_031965159.1
Accession Version	XM_031965158.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCC TGTATGTGTC TCCTCACCCG GACGCCTTCC CCAGCCTTCG CGCCCTGATT 
GCTGCCCGAT ACGGGGAGTC TGGAACTGGC CCAGGGTGGG GAGGGCCTCC TCCCCGCGTC 
TGCCTCCAGG CACCCCCTAC CAGCGGCACT TCCATCCCCC CGCCTCGGCT GCCGGTTCTC 
GAGCAGGGAC CAGGGGGCTT AAGGGTATGG GGCGCGGCCG CTGTCGCGCA GCTCCTGTGG 
CCCGCGGGAA TGGGGGGTCC CGGAGGCAGC CGAGGAGCCA CCCTTGTCCA GCAGTGGGTC 
AGCTATGCGG ATGGAGAGCT CGTGCCCGCT GGCTGTGGAG CCGCGCTGCC CGCTTTAGGG 
CTTCGGAACC CTAATCACGA CCCTCAGGCA GCCCTGGGAG CCCTGGGACG GGCTCTGGGC 
CCATTGGAGG AGAGACTGAG GTTGCATACC TATTTGGTAG GAGAAGCCCC CACTTTGGCC 
GACCTGGCAG CTGTTACAGC CCTACTGCTG CCCTTCCGCT ATGTTCTAGA CCCTCCTGCT 
CGCTGGGCTT GGGGTAACGT AAGCCGCTGG TTCATGACAT GTGTCCAGCA ACCAGAATTT 
CGTGCTGTGT TAGGGGATGT GTCCCTGTGC CCAGGAGTGC GGTCTCTCCC ACAGCAACCT 
GGCTCTGAGG TTTCTGGGTC CTCCAAGACA GCAGCCCAGT TGAAAAAAGA AGCAAAAAAG 
CGGGAAAAGC TGGAGAAGTT TCAACAGAAG CAAAGGAACC AATTGCATCA GCCACCTCCT 
GGAGAGAAGA AGCCAAAAAT TGAGAAGAAG GAGAAACGGG ACCCTGGGGT CATAACATAT 
GATATCCCCA CCCCACCAGG GGAGAAGAAA GATGTAAGTG TGTCCATGCC TGACTCCTAT 
AGTCCCCAGT ATGTTGAAGC TGCTTGGTAT TCATGGTGGG AGAGCCAAGG ATTCTTCAAA 
CCAGAGTATG GGCGTGCCAG TGTGTCAGAG CCTAATCCTC GAGGTGTCTT CATGATGTGC 
ATCCCGCCTC CCAATGTGAC AGGGTCCCTG CACCTTGGCC ATGCCCTCAC CAATGCCATC 
CAGGATTCAC TGACCCGCTG GCACCGAATG AGAGGAGAAA CAACCCTGTG GAACCCAGGC 
TGTGATCATG CTGGTATTGC CACACAGGTA GTTGTGGAAA AGAAACTATG GAGGGAACAT 
GGGCTAAGCC GCCACCAGCT TGGTCGAGAG GCCTTCCTAC AAGAGGTCTG GAAATGGAAG 
AATGAGAAGG GAGACCGGAT TTACCATCAG CTAAGGAAGC TTGGTGGCTC CTTGGATTGG 
GATCGAGCTT GTTTCACCAT GGATCCTAAA CTCTCAGCAG CTGTGACAGA AGCTTTTGTT 
CGCCTCCATA ATGATGGGGT CATTTACCGG AGTACCCGCC TTGTCAACTG GTCCTGTAGT 
CTCAACTCTG CCATTTCTGA CATTGAGGTA GATAAGAAGG AACTGTCGGG CCGAACCTTG 
CTTTCTGTCC CAGGCTATCA GGAGAAGGTG GAATTTGGGG TCATTGTTTC TTTTGCCTAC 
AAGATACAGG ATTCAGAGAG CAATGAGGAG GTGGTCGTAG CAACTACTCG TATTGAGACC 
ATGTTGGGAG ATGTAGCTGT GGCTGTGCAC CCCAATGACC CTAGATACCA GCACCTTCAG 
GGAAAGAGTG TTATACACCC ATTCCTTGCT CGAAGCCTTC CTATCATCTT TGATGAATTT 
GTGGACATGG AGTTTGGCAC TGGTGCTGTA AAAATTACAC CAGCCCATGA TCAGAATGAC 
TATGAGGTTG GGCAAAGACA CAAGCTAGAA GCAGTGAGCA TCATAGACCA CCAAGGCAAC 
CTTATCAATG TTCCGCCTCC CTTTCTGGGC CTTCCTCGGT TTGAAGCCAG GAAAGCAGTG 
TTGGCAGCCC TAAAAGACAA GGGACTATTC CGGGAAGTGA AGGACAATCC AATGGTGGTA 
CCTTTGTGCA ACCGCTCCAA AGATGTCGTT GAACCCCTCC TTAAGCCCCA GTGGTATGTG 
AGGTGTGGTG AAATGGCTCA GGCAGCCAGT GCAGCAGTGA CTCGGGGTGA CCTTAAGATA 
TTACCTGAGA TTCATCAGAA AATATGGCAC AATTGGATGG ACAACATCAG AGACTGGTGC 
ATCTCCCGGC AGCTGTGGTG GGGTCACCGA ATCCCTGCCT ACTTTGTCAC TGTCAATGAC 
CCTAAAGTGC CCCCTGGGGA GGATCCTGAT GGTCGGTATT GGGTGAGTGG CCGAAGTGAA 
GAGGAGGCGA GGGAGAAGGC AGCAAAGAAG TTTGGAGTGT CCACAGACAA GATTAGCCTC 
TCACAGGATG AAGATGTTTT AGACACATGG TTTTCCTCTG GCATTTTTCC CTTTTCCATC 
TTTGGATGGC CCAACCAGAC AGAGGACTTA AGTATATTCT ACCCAGGGAC CTTGTTGGAG 
ACAGGACATG ACATTTTATT CTTCTGGGTA GCCCGCATGG TGATGCTGGG TCTGAAGCTA 
ACAGGAAAAC TGCCCTTCAA AGAGGTTTAT CTCCATGCCC TCGTCAGAGA TGCCCATGGC 
CGGAAAATGA GCAAATCACT TGGTAATGTC ATTGACCCCC TGGATGTCAT CAGTGGACTC 
TCCCTGCAGG GACTGCATGA TCAGCTACTG AACAGCAACC TTGACCCCAG TGAAATGGAG 
AAAGCCAAGG AGGGGCAGAA GGCAGATTTC CCCAATGGAA TCCCTGAATG TGGTACAGAT 
GCTCTCAGAT TTGGACTTTG TGCTTACACT TCTCAGGGCC GGGACATTAA CCTGGATGTG 
AACCGAATCC TTGGGTATCG ACATTTCTGT AATAAGCTCT GGAATGCCAC CAAGTTTGCC 
CTTAGGGCAT TGGGAGATGG ATTTGTACCC TCACCTACTC CCCAAGCTAG CAATCAGGAG 
AGCTTGGCTG ATCGTTGGAT TAGGAGCCGC CTGAGTGAAG CTGTTGCGCT GAGCGACCAG 
GGTTTCCATG CCTACGATTT CCCTACTGTC ACCACTGCCC AGTATAGCTT TTGGCTCTAT 
GACCTCTGCA ATGTCTACCT GGAGTGCTTG AAACCTGTGT TGTCTGGGAC AGACCAAGCT 
GCAGCAGATA CTGCCCGGCA GACCCTCTAT ACTTGCCTGG ATGTGGGCCT AAGACTACTC 
TCACCCTTCA TGCCTTTTGT GACTGAGGAA CTGTATCAGC GATTGCCGCG GCGGGGGCCC 
CAGATGCCAC CCAGTCTTTG TGTTACCCCC TACCCAGAGC CCACTGAGTT CTCCTGGAAG 
GATCCCGAGG CTGAGGCTGC TTTTGAGCTT GCACTTAGTA TTATCCGGGC TGTGCGTTCT 
CTCCGAGCTG ATTATAACCT CACCCGAAGC CAACCTGAAT GTTTTCTGGA AGTGGTGGAT 
GAGGCAACAG GAGTACAGGC TTCTACAGTG TCAGGCTATG TGCAGGCTCT CTCCAACACT 
GGAGCTGTGA CTATTCTGCT CCCTGGGTCC CCTGCTCCAC AGGGTTGTGC TGTGGGCCTG 
GCCTCTGACC GATGCTCTGT CCACCTGCAG CTTCAGGGGC TGGTGGATCC TTCCCGGGAG 
CTGGCAAAAC TCCGGGCCAA ACAAGGGGAA GCTGAGCATC AGGCCCAAAG GCTTCGGGAG 
CGTCGGGCTG TTCCTGATTA TACCACCAAG GTTCCTTTCC AGGTCCAGGA GTCTGAGGAA 
GCTAAGCTCC AACAGACAGA GGCAGAGCTG AAGAAGGTAG ATGAAGCTAT TGCCCTGTTT 
GAGAAGATGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031821018.1
CDS	326..4120
Translation	MSILYVSPHPDAFPSLRALIAARYGESGTGPGWGGPPPRVCLQAPPTSGTSIPPPRLPVLEQGPGGLRVWGAAAVAQLLWPAGMGGPGGSRGATLVQQWVSYADGELVPAGCGAALPALGLRNPNHDPQAALGALGRALGPLEERLRLHTYLVGEAPTLADLAAVTALLLPFRYVLDPPARWAWGNVSRWFMTCVQQPEFRAVLGDVSLCPGVRSLPQQPGSEVSGSSKTAAQLKKEAKKREKLEKFQQKQRNQLHQPPPGEKKPKIEKKEKRDPGVITYDIPTPPGEKKDVSVSMPDSYSPQYVEAAWYSWWESQGFFKPEYGRASVSEPNPRGVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREHGLSRHQLGREAFLQEVWKWKNEKGDRIYHQLRKLGGSLDWDRACFTMDPKLSAAVTEAFVRLHNDGVIYRSTRLVNWSCSLNSAISDIEVDKKELSGRTLLSVPGYQEKVEFGVIVSFAYKIQDSESNEEVVVATTRIETMLGDVAVAVHPNDPRYQHLQGKSVIHPFLARSLPIIFDEFVDMEFGTGAVKITPAHDQNDYEVGQRHKLEAVSIIDHQGNLINVPPPFLGLPRFEARKAVLAALKDKGLFREVKDNPMVVPLCNRSKDVVEPLLKPQWYVRCGEMAQAASAAVTRGDLKILPEIHQKIWHNWMDNIRDWCISRQLWWGHRIPAYFVTVNDPKVPPGEDPDGRYWVSGRSEEEAREKAAKKFGVSTDKISLSQDEDVLDTWFSSGIFPFSIFGWPNQTEDLSIFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFKEVYLHALVRDAHGRKMSKSLGNVIDPLDVISGLSLQGLHDQLLNSNLDPSEMEKAKEGQKADFPNGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRALGDGFVPSPTPQASNQESLADRWIRSRLSEAVALSDQGFHAYDFPTVTTAQYSFWLYDLCNVYLECLKPVLSGTDQAAADTARQTLYTCLDVGLRLLSPFMPFVTEELYQRLPRRGPQMPPSLCVTPYPEPTEFSWKDPEAEAAFELALSIIRAVRSLRADYNLTRSQPECFLEVVDEATGVQASTVSGYVQALSNTGAVTILLPGSPAPQGCAVGLASDRCSVHLQLQGLVDPSRELAKLRAKQGEAEHQAQRLRERRAVPDYTTKVPFQVQESEEAKLQQTEAELKKVDEAIALFEKML

Target ORF information:

RefSeq Version	XM_031965158.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii valyl-tRNA synthetase 1 (VARS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031965158.1

ORF Insert Sequence:

ATGTCCATCC TGTATGTGTC TCCTCACCCG GACGCCTTCC CCAGCCTTCG CGCCCTGATT 
GCTGCCCGAT ACGGGGAGTC TGGAACTGGC CCAGGGTGGG GAGGGCCTCC TCCCCGCGTC 
TGCCTCCAGG CACCCCCTAC CAGCGGCACT TCCATCCCCC CGCCTCGGCT GCCGGTTCTC 
GAGCAGGGAC CAGGGGGCTT AAGGGTATGG GGCGCGGCCG CTGTCGCGCA GCTCCTGTGG 
CCCGCGGGAA TGGGGGGTCC CGGAGGCAGC CGAGGAGCCA CCCTTGTCCA GCAGTGGGTC 
AGCTATGCGG ATGGAGAGCT CGTGCCCGCT GGCTGTGGAG CCGCGCTGCC CGCTTTAGGG 
CTTCGGAACC CTAATCACGA CCCTCAGGCA GCCCTGGGAG CCCTGGGACG GGCTCTGGGC 
CCATTGGAGG AGAGACTGAG GTTGCATACC TATTTGGTAG GAGAAGCCCC CACTTTGGCC 
GACCTGGCAG CTGTTACAGC CCTACTGCTG CCCTTCCGCT ATGTTCTAGA CCCTCCTGCT 
CGCTGGGCTT GGGGTAACGT AAGCCGCTGG TTCATGACAT GTGTCCAGCA ACCAGAATTT 
CGTGCTGTGT TAGGGGATGT GTCCCTGTGC CCAGGAGTGC GGTCTCTCCC ACAGCAACCT 
GGCTCTGAGG TTTCTGGGTC CTCCAAGACA GCAGCCCAGT TGAAAAAAGA AGCAAAAAAG 
CGGGAAAAGC TGGAGAAGTT TCAACAGAAG CAAAGGAACC AATTGCATCA GCCACCTCCT 
GGAGAGAAGA AGCCAAAAAT TGAGAAGAAG GAGAAACGGG ACCCTGGGGT CATAACATAT 
GATATCCCCA CCCCACCAGG GGAGAAGAAA GATGTAAGTG TGTCCATGCC TGACTCCTAT 
AGTCCCCAGT ATGTTGAAGC TGCTTGGTAT TCATGGTGGG AGAGCCAAGG ATTCTTCAAA 
CCAGAGTATG GGCGTGCCAG TGTGTCAGAG CCTAATCCTC GAGGTGTCTT CATGATGTGC 
ATCCCGCCTC CCAATGTGAC AGGGTCCCTG CACCTTGGCC ATGCCCTCAC CAATGCCATC 
CAGGATTCAC TGACCCGCTG GCACCGAATG AGAGGAGAAA CAACCCTGTG GAACCCAGGC 
TGTGATCATG CTGGTATTGC CACACAGGTA GTTGTGGAAA AGAAACTATG GAGGGAACAT 
GGGCTAAGCC GCCACCAGCT TGGTCGAGAG GCCTTCCTAC AAGAGGTCTG GAAATGGAAG 
AATGAGAAGG GAGACCGGAT TTACCATCAG CTAAGGAAGC TTGGTGGCTC CTTGGATTGG 
GATCGAGCTT GTTTCACCAT GGATCCTAAA CTCTCAGCAG CTGTGACAGA AGCTTTTGTT 
CGCCTCCATA ATGATGGGGT CATTTACCGG AGTACCCGCC TTGTCAACTG GTCCTGTAGT 
CTCAACTCTG CCATTTCTGA CATTGAGGTA GATAAGAAGG AACTGTCGGG CCGAACCTTG 
CTTTCTGTCC CAGGCTATCA GGAGAAGGTG GAATTTGGGG TCATTGTTTC TTTTGCCTAC 
AAGATACAGG ATTCAGAGAG CAATGAGGAG GTGGTCGTAG CAACTACTCG TATTGAGACC 
ATGTTGGGAG ATGTAGCTGT GGCTGTGCAC CCCAATGACC CTAGATACCA GCACCTTCAG 
GGAAAGAGTG TTATACACCC ATTCCTTGCT CGAAGCCTTC CTATCATCTT TGATGAATTT 
GTGGACATGG AGTTTGGCAC TGGTGCTGTA AAAATTACAC CAGCCCATGA TCAGAATGAC 
TATGAGGTTG GGCAAAGACA CAAGCTAGAA GCAGTGAGCA TCATAGACCA CCAAGGCAAC 
CTTATCAATG TTCCGCCTCC CTTTCTGGGC CTTCCTCGGT TTGAAGCCAG GAAAGCAGTG 
TTGGCAGCCC TAAAAGACAA GGGACTATTC CGGGAAGTGA AGGACAATCC AATGGTGGTA 
CCTTTGTGCA ACCGCTCCAA AGATGTCGTT GAACCCCTCC TTAAGCCCCA GTGGTATGTG 
AGGTGTGGTG AAATGGCTCA GGCAGCCAGT GCAGCAGTGA CTCGGGGTGA CCTTAAGATA 
TTACCTGAGA TTCATCAGAA AATATGGCAC AATTGGATGG ACAACATCAG AGACTGGTGC 
ATCTCCCGGC AGCTGTGGTG GGGTCACCGA ATCCCTGCCT ACTTTGTCAC TGTCAATGAC 
CCTAAAGTGC CCCCTGGGGA GGATCCTGAT GGTCGGTATT GGGTGAGTGG CCGAAGTGAA 
GAGGAGGCGA GGGAGAAGGC AGCAAAGAAG TTTGGAGTGT CCACAGACAA GATTAGCCTC 
TCACAGGATG AAGATGTTTT AGACACATGG TTTTCCTCTG GCATTTTTCC CTTTTCCATC 
TTTGGATGGC CCAACCAGAC AGAGGACTTA AGTATATTCT ACCCAGGGAC CTTGTTGGAG 
ACAGGACATG ACATTTTATT CTTCTGGGTA GCCCGCATGG TGATGCTGGG TCTGAAGCTA 
ACAGGAAAAC TGCCCTTCAA AGAGGTTTAT CTCCATGCCC TCGTCAGAGA TGCCCATGGC 
CGGAAAATGA GCAAATCACT TGGTAATGTC ATTGACCCCC TGGATGTCAT CAGTGGACTC 
TCCCTGCAGG GACTGCATGA TCAGCTACTG AACAGCAACC TTGACCCCAG TGAAATGGAG 
AAAGCCAAGG AGGGGCAGAA GGCAGATTTC CCCAATGGAA TCCCTGAATG TGGTACAGAT 
GCTCTCAGAT TTGGACTTTG TGCTTACACT TCTCAGGGCC GGGACATTAA CCTGGATGTG 
AACCGAATCC TTGGGTATCG ACATTTCTGT AATAAGCTCT GGAATGCCAC CAAGTTTGCC 
CTTAGGGCAT TGGGAGATGG ATTTGTACCC TCACCTACTC CCCAAGCTAG CAATCAGGAG 
AGCTTGGCTG ATCGTTGGAT TAGGAGCCGC CTGAGTGAAG CTGTTGCGCT GAGCGACCAG 
GGTTTCCATG CCTACGATTT CCCTACTGTC ACCACTGCCC AGTATAGCTT TTGGCTCTAT 
GACCTCTGCA ATGTCTACCT GGAGTGCTTG AAACCTGTGT TGTCTGGGAC AGACCAAGCT 
GCAGCAGATA CTGCCCGGCA GACCCTCTAT ACTTGCCTGG ATGTGGGCCT AAGACTACTC 
TCACCCTTCA TGCCTTTTGT GACTGAGGAA CTGTATCAGC GATTGCCGCG GCGGGGGCCC 
CAGATGCCAC CCAGTCTTTG TGTTACCCCC TACCCAGAGC CCACTGAGTT CTCCTGGAAG 
GATCCCGAGG CTGAGGCTGC TTTTGAGCTT GCACTTAGTA TTATCCGGGC TGTGCGTTCT 
CTCCGAGCTG ATTATAACCT CACCCGAAGC CAACCTGAAT GTTTTCTGGA AGTGGTGGAT 
GAGGCAACAG GAGTACAGGC TTCTACAGTG TCAGGCTATG TGCAGGCTCT CTCCAACACT 
GGAGCTGTGA CTATTCTGCT CCCTGGGTCC CCTGCTCCAC AGGGTTGTGC TGTGGGCCTG 
GCCTCTGACC GATGCTCTGT CCACCTGCAG CTTCAGGGGC TGGTGGATCC TTCCCGGGAG 
CTGGCAAAAC TCCGGGCCAA ACAAGGGGAA GCTGAGCATC AGGCCCAAAG GCTTCGGGAG 
CGTCGGGCTG TTCCTGATTA TACCACCAAG GTTCCTTTCC AGGTCCAGGA GTCTGAGGAA 
GCTAAGCTCC AACAGACAGA GGCAGAGCTG AAGAAGGTAG ATGAAGCTAT TGCCCTGTTT 
GAGAAGATGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS ( XM_031965159.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031821019.1 Sarcophilus harrisii valyl-tRNA synthetase 1 (VARS1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSa233182
Clone ID Related Accession (Same CDS sequence)	XM_031965157.1 , XM_031965158.1 , XM_031965159.1
Accession Version	XM_031965159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCC TGTATGTGTC TCCTCACCCG GACGCCTTCC CCAGCCTTCG CGCCCTGATT 
GCTGCCCGAT ACGGGGAGTC TGGAACTGGC CCAGGGTGGG GAGGGCCTCC TCCCCGCGTC 
TGCCTCCAGG CACCCCCTAC CAGCGGCACT TCCATCCCCC CGCCTCGGCT GCCGGTTCTC 
GAGCAGGGAC CAGGGGGCTT AAGGGTATGG GGCGCGGCCG CTGTCGCGCA GCTCCTGTGG 
CCCGCGGGAA TGGGGGGTCC CGGAGGCAGC CGAGGAGCCA CCCTTGTCCA GCAGTGGGTC 
AGCTATGCGG ATGGAGAGCT CGTGCCCGCT GGCTGTGGAG CCGCGCTGCC CGCTTTAGGG 
CTTCGGAACC CTAATCACGA CCCTCAGGCA GCCCTGGGAG CCCTGGGACG GGCTCTGGGC 
CCATTGGAGG AGAGACTGAG GTTGCATACC TATTTGGTAG GAGAAGCCCC CACTTTGGCC 
GACCTGGCAG CTGTTACAGC CCTACTGCTG CCCTTCCGCT ATGTTCTAGA CCCTCCTGCT 
CGCTGGGCTT GGGGTAACGT AAGCCGCTGG TTCATGACAT GTGTCCAGCA ACCAGAATTT 
CGTGCTGTGT TAGGGGATGT GTCCCTGTGC CCAGGAGTGC GGTCTCTCCC ACAGCAACCT 
GGCTCTGAGG TTTCTGGGTC CTCCAAGACA GCAGCCCAGT TGAAAAAAGA AGCAAAAAAG 
CGGGAAAAGC TGGAGAAGTT TCAACAGAAG CAAAGGAACC AATTGCATCA GCCACCTCCT 
GGAGAGAAGA AGCCAAAAAT TGAGAAGAAG GAGAAACGGG ACCCTGGGGT CATAACATAT 
GATATCCCCA CCCCACCAGG GGAGAAGAAA GATGTAAGTG TGTCCATGCC TGACTCCTAT 
AGTCCCCAGT ATGTTGAAGC TGCTTGGTAT TCATGGTGGG AGAGCCAAGG ATTCTTCAAA 
CCAGAGTATG GGCGTGCCAG TGTGTCAGAG CCTAATCCTC GAGGTGTCTT CATGATGTGC 
ATCCCGCCTC CCAATGTGAC AGGGTCCCTG CACCTTGGCC ATGCCCTCAC CAATGCCATC 
CAGGATTCAC TGACCCGCTG GCACCGAATG AGAGGAGAAA CAACCCTGTG GAACCCAGGC 
TGTGATCATG CTGGTATTGC CACACAGGTA GTTGTGGAAA AGAAACTATG GAGGGAACAT 
GGGCTAAGCC GCCACCAGCT TGGTCGAGAG GCCTTCCTAC AAGAGGTCTG GAAATGGAAG 
AATGAGAAGG GAGACCGGAT TTACCATCAG CTAAGGAAGC TTGGTGGCTC CTTGGATTGG 
GATCGAGCTT GTTTCACCAT GGATCCTAAA CTCTCAGCAG CTGTGACAGA AGCTTTTGTT 
CGCCTCCATA ATGATGGGGT CATTTACCGG AGTACCCGCC TTGTCAACTG GTCCTGTAGT 
CTCAACTCTG CCATTTCTGA CATTGAGGTA GATAAGAAGG AACTGTCGGG CCGAACCTTG 
CTTTCTGTCC CAGGCTATCA GGAGAAGGTG GAATTTGGGG TCATTGTTTC TTTTGCCTAC 
AAGATACAGG ATTCAGAGAG CAATGAGGAG GTGGTCGTAG CAACTACTCG TATTGAGACC 
ATGTTGGGAG ATGTAGCTGT GGCTGTGCAC CCCAATGACC CTAGATACCA GCACCTTCAG 
GGAAAGAGTG TTATACACCC ATTCCTTGCT CGAAGCCTTC CTATCATCTT TGATGAATTT 
GTGGACATGG AGTTTGGCAC TGGTGCTGTA AAAATTACAC CAGCCCATGA TCAGAATGAC 
TATGAGGTTG GGCAAAGACA CAAGCTAGAA GCAGTGAGCA TCATAGACCA CCAAGGCAAC 
CTTATCAATG TTCCGCCTCC CTTTCTGGGC CTTCCTCGGT TTGAAGCCAG GAAAGCAGTG 
TTGGCAGCCC TAAAAGACAA GGGACTATTC CGGGAAGTGA AGGACAATCC AATGGTGGTA 
CCTTTGTGCA ACCGCTCCAA AGATGTCGTT GAACCCCTCC TTAAGCCCCA GTGGTATGTG 
AGGTGTGGTG AAATGGCTCA GGCAGCCAGT GCAGCAGTGA CTCGGGGTGA CCTTAAGATA 
TTACCTGAGA TTCATCAGAA AATATGGCAC AATTGGATGG ACAACATCAG AGACTGGTGC 
ATCTCCCGGC AGCTGTGGTG GGGTCACCGA ATCCCTGCCT ACTTTGTCAC TGTCAATGAC 
CCTAAAGTGC CCCCTGGGGA GGATCCTGAT GGTCGGTATT GGGTGAGTGG CCGAAGTGAA 
GAGGAGGCGA GGGAGAAGGC AGCAAAGAAG TTTGGAGTGT CCACAGACAA GATTAGCCTC 
TCACAGGATG AAGATGTTTT AGACACATGG TTTTCCTCTG GCATTTTTCC CTTTTCCATC 
TTTGGATGGC CCAACCAGAC AGAGGACTTA AGTATATTCT ACCCAGGGAC CTTGTTGGAG 
ACAGGACATG ACATTTTATT CTTCTGGGTA GCCCGCATGG TGATGCTGGG TCTGAAGCTA 
ACAGGAAAAC TGCCCTTCAA AGAGGTTTAT CTCCATGCCC TCGTCAGAGA TGCCCATGGC 
CGGAAAATGA GCAAATCACT TGGTAATGTC ATTGACCCCC TGGATGTCAT CAGTGGACTC 
TCCCTGCAGG GACTGCATGA TCAGCTACTG AACAGCAACC TTGACCCCAG TGAAATGGAG 
AAAGCCAAGG AGGGGCAGAA GGCAGATTTC CCCAATGGAA TCCCTGAATG TGGTACAGAT 
GCTCTCAGAT TTGGACTTTG TGCTTACACT TCTCAGGGCC GGGACATTAA CCTGGATGTG 
AACCGAATCC TTGGGTATCG ACATTTCTGT AATAAGCTCT GGAATGCCAC CAAGTTTGCC 
CTTAGGGCAT TGGGAGATGG ATTTGTACCC TCACCTACTC CCCAAGCTAG CAATCAGGAG 
AGCTTGGCTG ATCGTTGGAT TAGGAGCCGC CTGAGTGAAG CTGTTGCGCT GAGCGACCAG 
GGTTTCCATG CCTACGATTT CCCTACTGTC ACCACTGCCC AGTATAGCTT TTGGCTCTAT 
GACCTCTGCA ATGTCTACCT GGAGTGCTTG AAACCTGTGT TGTCTGGGAC AGACCAAGCT 
GCAGCAGATA CTGCCCGGCA GACCCTCTAT ACTTGCCTGG ATGTGGGCCT AAGACTACTC 
TCACCCTTCA TGCCTTTTGT GACTGAGGAA CTGTATCAGC GATTGCCGCG GCGGGGGCCC 
CAGATGCCAC CCAGTCTTTG TGTTACCCCC TACCCAGAGC CCACTGAGTT CTCCTGGAAG 
GATCCCGAGG CTGAGGCTGC TTTTGAGCTT GCACTTAGTA TTATCCGGGC TGTGCGTTCT 
CTCCGAGCTG ATTATAACCT CACCCGAAGC CAACCTGAAT GTTTTCTGGA AGTGGTGGAT 
GAGGCAACAG GAGTACAGGC TTCTACAGTG TCAGGCTATG TGCAGGCTCT CTCCAACACT 
GGAGCTGTGA CTATTCTGCT CCCTGGGTCC CCTGCTCCAC AGGGTTGTGC TGTGGGCCTG 
GCCTCTGACC GATGCTCTGT CCACCTGCAG CTTCAGGGGC TGGTGGATCC TTCCCGGGAG 
CTGGCAAAAC TCCGGGCCAA ACAAGGGGAA GCTGAGCATC AGGCCCAAAG GCTTCGGGAG 
CGTCGGGCTG TTCCTGATTA TACCACCAAG GTTCCTTTCC AGGTCCAGGA GTCTGAGGAA 
GCTAAGCTCC AACAGACAGA GGCAGAGCTG AAGAAGGTAG ATGAAGCTAT TGCCCTGTTT 
GAGAAGATGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031821019.1
CDS	307..4101
Translation	MSILYVSPHPDAFPSLRALIAARYGESGTGPGWGGPPPRVCLQAPPTSGTSIPPPRLPVLEQGPGGLRVWGAAAVAQLLWPAGMGGPGGSRGATLVQQWVSYADGELVPAGCGAALPALGLRNPNHDPQAALGALGRALGPLEERLRLHTYLVGEAPTLADLAAVTALLLPFRYVLDPPARWAWGNVSRWFMTCVQQPEFRAVLGDVSLCPGVRSLPQQPGSEVSGSSKTAAQLKKEAKKREKLEKFQQKQRNQLHQPPPGEKKPKIEKKEKRDPGVITYDIPTPPGEKKDVSVSMPDSYSPQYVEAAWYSWWESQGFFKPEYGRASVSEPNPRGVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREHGLSRHQLGREAFLQEVWKWKNEKGDRIYHQLRKLGGSLDWDRACFTMDPKLSAAVTEAFVRLHNDGVIYRSTRLVNWSCSLNSAISDIEVDKKELSGRTLLSVPGYQEKVEFGVIVSFAYKIQDSESNEEVVVATTRIETMLGDVAVAVHPNDPRYQHLQGKSVIHPFLARSLPIIFDEFVDMEFGTGAVKITPAHDQNDYEVGQRHKLEAVSIIDHQGNLINVPPPFLGLPRFEARKAVLAALKDKGLFREVKDNPMVVPLCNRSKDVVEPLLKPQWYVRCGEMAQAASAAVTRGDLKILPEIHQKIWHNWMDNIRDWCISRQLWWGHRIPAYFVTVNDPKVPPGEDPDGRYWVSGRSEEEAREKAAKKFGVSTDKISLSQDEDVLDTWFSSGIFPFSIFGWPNQTEDLSIFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFKEVYLHALVRDAHGRKMSKSLGNVIDPLDVISGLSLQGLHDQLLNSNLDPSEMEKAKEGQKADFPNGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRALGDGFVPSPTPQASNQESLADRWIRSRLSEAVALSDQGFHAYDFPTVTTAQYSFWLYDLCNVYLECLKPVLSGTDQAAADTARQTLYTCLDVGLRLLSPFMPFVTEELYQRLPRRGPQMPPSLCVTPYPEPTEFSWKDPEAEAAFELALSIIRAVRSLRADYNLTRSQPECFLEVVDEATGVQASTVSGYVQALSNTGAVTILLPGSPAPQGCAVGLASDRCSVHLQLQGLVDPSRELAKLRAKQGEAEHQAQRLRERRAVPDYTTKVPFQVQESEEAKLQQTEAELKKVDEAIALFEKML

Target ORF information:

RefSeq Version	XM_031965159.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii valyl-tRNA synthetase 1 (VARS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031965159.1

ORF Insert Sequence:

ATGTCCATCC TGTATGTGTC TCCTCACCCG GACGCCTTCC CCAGCCTTCG CGCCCTGATT 
GCTGCCCGAT ACGGGGAGTC TGGAACTGGC CCAGGGTGGG GAGGGCCTCC TCCCCGCGTC 
TGCCTCCAGG CACCCCCTAC CAGCGGCACT TCCATCCCCC CGCCTCGGCT GCCGGTTCTC 
GAGCAGGGAC CAGGGGGCTT AAGGGTATGG GGCGCGGCCG CTGTCGCGCA GCTCCTGTGG 
CCCGCGGGAA TGGGGGGTCC CGGAGGCAGC CGAGGAGCCA CCCTTGTCCA GCAGTGGGTC 
AGCTATGCGG ATGGAGAGCT CGTGCCCGCT GGCTGTGGAG CCGCGCTGCC CGCTTTAGGG 
CTTCGGAACC CTAATCACGA CCCTCAGGCA GCCCTGGGAG CCCTGGGACG GGCTCTGGGC 
CCATTGGAGG AGAGACTGAG GTTGCATACC TATTTGGTAG GAGAAGCCCC CACTTTGGCC 
GACCTGGCAG CTGTTACAGC CCTACTGCTG CCCTTCCGCT ATGTTCTAGA CCCTCCTGCT 
CGCTGGGCTT GGGGTAACGT AAGCCGCTGG TTCATGACAT GTGTCCAGCA ACCAGAATTT 
CGTGCTGTGT TAGGGGATGT GTCCCTGTGC CCAGGAGTGC GGTCTCTCCC ACAGCAACCT 
GGCTCTGAGG TTTCTGGGTC CTCCAAGACA GCAGCCCAGT TGAAAAAAGA AGCAAAAAAG 
CGGGAAAAGC TGGAGAAGTT TCAACAGAAG CAAAGGAACC AATTGCATCA GCCACCTCCT 
GGAGAGAAGA AGCCAAAAAT TGAGAAGAAG GAGAAACGGG ACCCTGGGGT CATAACATAT 
GATATCCCCA CCCCACCAGG GGAGAAGAAA GATGTAAGTG TGTCCATGCC TGACTCCTAT 
AGTCCCCAGT ATGTTGAAGC TGCTTGGTAT TCATGGTGGG AGAGCCAAGG ATTCTTCAAA 
CCAGAGTATG GGCGTGCCAG TGTGTCAGAG CCTAATCCTC GAGGTGTCTT CATGATGTGC 
ATCCCGCCTC CCAATGTGAC AGGGTCCCTG CACCTTGGCC ATGCCCTCAC CAATGCCATC 
CAGGATTCAC TGACCCGCTG GCACCGAATG AGAGGAGAAA CAACCCTGTG GAACCCAGGC 
TGTGATCATG CTGGTATTGC CACACAGGTA GTTGTGGAAA AGAAACTATG GAGGGAACAT 
GGGCTAAGCC GCCACCAGCT TGGTCGAGAG GCCTTCCTAC AAGAGGTCTG GAAATGGAAG 
AATGAGAAGG GAGACCGGAT TTACCATCAG CTAAGGAAGC TTGGTGGCTC CTTGGATTGG 
GATCGAGCTT GTTTCACCAT GGATCCTAAA CTCTCAGCAG CTGTGACAGA AGCTTTTGTT 
CGCCTCCATA ATGATGGGGT CATTTACCGG AGTACCCGCC TTGTCAACTG GTCCTGTAGT 
CTCAACTCTG CCATTTCTGA CATTGAGGTA GATAAGAAGG AACTGTCGGG CCGAACCTTG 
CTTTCTGTCC CAGGCTATCA GGAGAAGGTG GAATTTGGGG TCATTGTTTC TTTTGCCTAC 
AAGATACAGG ATTCAGAGAG CAATGAGGAG GTGGTCGTAG CAACTACTCG TATTGAGACC 
ATGTTGGGAG ATGTAGCTGT GGCTGTGCAC CCCAATGACC CTAGATACCA GCACCTTCAG 
GGAAAGAGTG TTATACACCC ATTCCTTGCT CGAAGCCTTC CTATCATCTT TGATGAATTT 
GTGGACATGG AGTTTGGCAC TGGTGCTGTA AAAATTACAC CAGCCCATGA TCAGAATGAC 
TATGAGGTTG GGCAAAGACA CAAGCTAGAA GCAGTGAGCA TCATAGACCA CCAAGGCAAC 
CTTATCAATG TTCCGCCTCC CTTTCTGGGC CTTCCTCGGT TTGAAGCCAG GAAAGCAGTG 
TTGGCAGCCC TAAAAGACAA GGGACTATTC CGGGAAGTGA AGGACAATCC AATGGTGGTA 
CCTTTGTGCA ACCGCTCCAA AGATGTCGTT GAACCCCTCC TTAAGCCCCA GTGGTATGTG 
AGGTGTGGTG AAATGGCTCA GGCAGCCAGT GCAGCAGTGA CTCGGGGTGA CCTTAAGATA 
TTACCTGAGA TTCATCAGAA AATATGGCAC AATTGGATGG ACAACATCAG AGACTGGTGC 
ATCTCCCGGC AGCTGTGGTG GGGTCACCGA ATCCCTGCCT ACTTTGTCAC TGTCAATGAC 
CCTAAAGTGC CCCCTGGGGA GGATCCTGAT GGTCGGTATT GGGTGAGTGG CCGAAGTGAA 
GAGGAGGCGA GGGAGAAGGC AGCAAAGAAG TTTGGAGTGT CCACAGACAA GATTAGCCTC 
TCACAGGATG AAGATGTTTT AGACACATGG TTTTCCTCTG GCATTTTTCC CTTTTCCATC 
TTTGGATGGC CCAACCAGAC AGAGGACTTA AGTATATTCT ACCCAGGGAC CTTGTTGGAG 
ACAGGACATG ACATTTTATT CTTCTGGGTA GCCCGCATGG TGATGCTGGG TCTGAAGCTA 
ACAGGAAAAC TGCCCTTCAA AGAGGTTTAT CTCCATGCCC TCGTCAGAGA TGCCCATGGC 
CGGAAAATGA GCAAATCACT TGGTAATGTC ATTGACCCCC TGGATGTCAT CAGTGGACTC 
TCCCTGCAGG GACTGCATGA TCAGCTACTG AACAGCAACC TTGACCCCAG TGAAATGGAG 
AAAGCCAAGG AGGGGCAGAA GGCAGATTTC CCCAATGGAA TCCCTGAATG TGGTACAGAT 
GCTCTCAGAT TTGGACTTTG TGCTTACACT TCTCAGGGCC GGGACATTAA CCTGGATGTG 
AACCGAATCC TTGGGTATCG ACATTTCTGT AATAAGCTCT GGAATGCCAC CAAGTTTGCC 
CTTAGGGCAT TGGGAGATGG ATTTGTACCC TCACCTACTC CCCAAGCTAG CAATCAGGAG 
AGCTTGGCTG ATCGTTGGAT TAGGAGCCGC CTGAGTGAAG CTGTTGCGCT GAGCGACCAG 
GGTTTCCATG CCTACGATTT CCCTACTGTC ACCACTGCCC AGTATAGCTT TTGGCTCTAT 
GACCTCTGCA ATGTCTACCT GGAGTGCTTG AAACCTGTGT TGTCTGGGAC AGACCAAGCT 
GCAGCAGATA CTGCCCGGCA GACCCTCTAT ACTTGCCTGG ATGTGGGCCT AAGACTACTC 
TCACCCTTCA TGCCTTTTGT GACTGAGGAA CTGTATCAGC GATTGCCGCG GCGGGGGCCC 
CAGATGCCAC CCAGTCTTTG TGTTACCCCC TACCCAGAGC CCACTGAGTT CTCCTGGAAG 
GATCCCGAGG CTGAGGCTGC TTTTGAGCTT GCACTTAGTA TTATCCGGGC TGTGCGTTCT 
CTCCGAGCTG ATTATAACCT CACCCGAAGC CAACCTGAAT GTTTTCTGGA AGTGGTGGAT 
GAGGCAACAG GAGTACAGGC TTCTACAGTG TCAGGCTATG TGCAGGCTCT CTCCAACACT 
GGAGCTGTGA CTATTCTGCT CCCTGGGTCC CCTGCTCCAC AGGGTTGTGC TGTGGGCCTG 
GCCTCTGACC GATGCTCTGT CCACCTGCAG CTTCAGGGGC TGGTGGATCC TTCCCGGGAG 
CTGGCAAAAC TCCGGGCCAA ACAAGGGGAA GCTGAGCATC AGGCCCAAAG GCTTCGGGAG 
CGTCGGGCTG TTCCTGATTA TACCACCAAG GTTCCTTTCC AGGTCCAGGA GTCTGAGGAA 
GCTAAGCTCC AACAGACAGA GGCAGAGCTG AAGAAGGTAG ATGAAGCTAT TGCCCTGTTT 
GAGAAGATGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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VARS cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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