URB1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	URB1
Entrez Gene ID	100928620
Full Name	URB1 ribosome biogenesis 1 homolog (S. cerevisiae)
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012544405.3	XP_012399859.1	nucleolar pre-ribosomal-associated protein 1 isoform X1
XM_012544405.2	XP_012399859.1	nucleolar pre-ribosomal-associated protein 1 isoform X1
XM_012544405.1	XP_012399859.1	nucleolar pre-ribosomal-associated protein 1
XM_023498904.1	XP_023354672.1	nucleolar pre-ribosomal-associated protein 1 isoform X2
XM_023498905.1	XP_023354673.1	nucleolar pre-ribosomal-associated protein 1 isoform X3
XM_023498904.2	XP_023354672.1	nucleolar pre-ribosomal-associated protein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following URB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the URB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa151556	XM_023498905.1 Latest version!	Sarcophilus harrisii URB1 ribosome biogenesis 1 homolog (S. cerevisiae) (URB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa125228	XM_012544405.3 Latest version!	Sarcophilus harrisii URB1 ribosome biogenesis homolog (URB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa151555	XM_023498904.2 Latest version!	Sarcophilus harrisii URB1 ribosome biogenesis homolog (URB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OSa125228	XM_012544405.2	Sarcophilus harrisii URB1 ribosome biogenesis 1 homolog (S. cerevisiae) (URB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa125228	XM_012544405.1	Sarcophilus harrisii URB1 ribosome biogenesis 1 homolog (S. cerevisiae) (URB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa151555	XM_023498904.1	Sarcophilus harrisii URB1 ribosome biogenesis 1 homolog (S. cerevisiae) (URB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

URB1 ( XM_023498905.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023354673.1 Sarcophilus harrisii URB1 ribosome biogenesis 1 homolog (S. cerevisiae) (URB1), transcript variant X3, mRNA.

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CloneID	OSa151556
Clone ID Related Accession (Same CDS sequence)	XM_023498905.1
Accession Version	XM_023498905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	nucleolar pre-ribosomal-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831550.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAGAG GCCTGCAATC ACTTAACAAA ATTTCAGTGG CTATTTATGA GGCTCAGCCA 
GAGATTTCCC CAGCATTTAA AACTAAAGAG TTTATACCCT TGTCTAGATT GCTTTCCATG 
GTCATGGTGA CAACAGTACC CAACGTGTGC AACAAAATCA TGTTTACTCA AGGATTAAAA 
TTGGAAAACA AATCAGTAAA TCATACAATT TTATCACTTA TTTCCACCAT TTTGAAGAGA 
GCATTAAAAA ACATTGATCA CTGTCTCAAT AAGGATATAT GGCAGGAGTC AAGCATCTAT 
ACTCCTGTGA TGATGGAGGA ATTTGTGCAT CTGTACAGAG AAGCTGTAAG CAAGCTTTTG 
CCAGATTTGA ACAATATTGT ATGTGTGTGG CAATCACTTG GAAAGCAAAA AATAGATGAA 
GATGGCGGGT TAGAAAAAAA AAATTCCCTT GAAGATGAGA AAACTGAAAC TACAGAAGTA 
GACCACAAAC ATGATGATGC AGAAACAACT CTTTTGAAGG CTATTTTACT TCAGGTCATT 
TGTCTTTATC AGATGGTTGT TCCCCATGTT GTCATTCAAT GTACATTTGA CTTCAGCAAG 
CTTCTAAAAG GTATCATTTC AGAAGAGGGA TTTCAAGAGG AAGTTCCTCC TGTCCTTCAG 
TACTATATTC TGAAAGTGGC CCTAGAATTG CCTGCCAGCA AATTTGCCTG GTTAAAAGGA 
CAGGAAAGTC CAAATAGTGG AGAAAGATCT GTGTTTTATT TGCTGATGAA AATGTTTGTG 
ACAAGTAAAC ACTCACAACT TAAAGCATCA ACAAAACTAT TGATTACAAA GATTTTACGT 
GACACTGGAG TATTTGAATA TACATGGAAA GAGCTAGAAC TATGGGTGGA GCAATTAGAT 
AACACAGCAG AAAACAGAAA AGAAGTTGTG ATTCAGTTTT TGGAATGTGT TTTGTTGCAA 
TTGGTAACAA ATCCCTATTC ATATACAGAC AAGGCAGCAG ATTTTGTTCA AGAAGCTAGT 
ACACTACAGG CCACTGTGGT GAAGCAAGAT GCTGATAATG TTAGCATACC TGTATCACAC 
ATTGATGATA TCCTGGATAT GGTGGATGTC CTCATGGAGG ACACTGAAGG CTTAGATGAG 
GAAGTTGGTT TCTCTTTAAA TGAAGATATG ATTCTGCTGA CATTCCCATT CAGCGCCATA 
GTTCCTACAG CTCTGGAGAG CAGGAATAAA TTGCTTCTTG GGAATGGAAA TGATTCAGGT 
GAAAGCATAG TGGAATATCT GATAGCCGTT CTGACAGACC TTCTACATAC ACAGCGAGAT 
CCTTTGGCTC TTTGTCTGAT GCTTCAGTCT TATGACAGTT TACAACTTTC AAACACATCT 
TTGTATACTC GGATTTACCA GTTCAACAGA TACTATAACC TGTGGATACC TGAGCAAGCC 
AGAGAAACTT TGGAATTTCA AGCTACCAGA CCCACTGAGA CATCACTTGT GGTTGGCTCT 
GCCTTTTCCA CCTTGTTAAC AGATACCTAT GAGAGGGGAG TTACCCTCCT TTTAGAAGAG 
AATATCCAGG GAAAACTGAG AGAAAAAATT GCTCAGCTCC CAATGCAGAA GGTTCTGCTC 
TCTGTGAAGC AGGTGATGCT GTATCTTAGA ACCACTGTGG AGAACTTTGC CAAGCTTGGT 
AAAAGTTCAG GTCCTGCTTT GCTTCAGCTC TTCATGAATT TGCTCAGTCA CCTGTTGAAC 
CATTGTTATC ATTTGGACAC ATGTAACCAG AAAAAATGTG AAGCTGCTCA GGCCGAATCT 
GCTATCTTTT TGGATGAGGA GTCCTTGGCC ACACTGGAAT TAGCCAATGA TAAGACTCTG 
GAAGATGCGT TGATAGTAAT CTTTAGACAT CCCACATTGG AGAGCTGGTA TTTGGCCCTG 
GAGCTGCAGA CTCTTCCCTC ACATAACCTC AATCCAATCC TTGTAAAGCT TTTAGCAACT 
CATTTCAATG CTGGGGTCCT CTCACTCCTG AAACTGAGTG CCCCAATTCT GCAGGAAATG 
CAACGTATGG ACATCCTTTC CAAGTATTTA GAAGCCATTA CTAAGAGTGT CTTGAAAGAA 
CTACACACCC TGAAGAAGAC AGATCCTCCC ATTGCATATG GGAAACCTTC AGCTCAGCTG 
GAAGCTTTGT ACCAACTACA TCCCTACATG GATGGAACCC AGCTTAGGGA CATTACGCTT 
GCACTCCTGG CTCTTCCTAA ATCAACCCTT TTGGATCAGG AATCTAAGAA ATCTCACCAA 
AAAAAAAAAC ATCTGAGCTT TTTAGGGGAA ACCCTCAAAC AGCTTATCAC TGAATCCTAC 
CAGGAACAAT CCCAGCAAGG TGAATTACTG TCGTCCCTAG AATATGTGAA GAGAATGAGT 
GTCCTGTTGC CTGCACTGGT CATTGAAGAG TTAGAGACTG TGTTCTTCCA AGCTTTACAG 
AAAGAGCCAA TTCTGGTCCA TGTAGTTGAG TTAGATTTAT TGACCTACTG TCTAAATCGG 
AGGACAAAAA CTTCTCTTTC CATTGTAGCT TTGTTGATTC AGCAAAGTAG TACTCACCTG 
CTACACTTTG AGTTGTGGTG CTTACAGGGC CTAGGGTCCT GCCTTCCAGA AAACTTCAGC 
CTCTTTCTTC CACTTATCCA TACCTATTTA CAGTCTTGTA TGCAGGGAGA CTTTACACGT 
CCTTCTAAAG TGTCTAGAAA TGTCATGAAT GTGTTGAGAA AGGTCCTGTG GAAAGAGCTT 
CAAAACAGTC TTTTAGGAGA TGATCCTCAG GATCTTGGAA TTCAAGAAGA GGTCCTTTCT 
AAATTGATTC CCTATTCAAG AGCAAAAGAA CTTAGACTCC TCATTGACAA AATGCCCAGC 
ATTCTTCAAA AGCCAGAGAA ATCGAAGAGC TGGATTGTAT CAGATGCCAT AGCATTAGTA 
TTGGAAAAAT CTGTGGAAGA GCTGGATGTT TGGAAAAAGA CTTTGTTGGG GTCATGTATA 
AAATGGCTAA TAGCTTCATA CAGCAGTAGT ATCAAAGAAG AAAATACCCA GGATGCAGAG 
AAGTCTATGT TGTTGCGATT TAATGAATTA TTGAACTCAG TTAATGAAGT TGATCCTGTT 
GATTGGCAGA AGTTTGTGAA GACAGGCCTT AAGTTCCGCT ACCATGATTA CACATTTTTA 
AAGACTCTTC ATGCTTGTAT ACTGCTGTTA TATCAGCCTG AAAATTCCCT TTGCAAAAAC 
TTAGTGCAGC TGCCACTAAT ATATGTGATG ATCACACAAC ACTCTATGTT TTTGCCAACC 
ATATTGCAAA CAAAAGAAGA TGACACAAAT ATCCAAGTAA AAGAAATGTT AGTGGACCTA 
ATATCAACTA TTGTAAAACT TTGCCCTTCC GTCTGTGAAA GCAACCACTT TGCAGTGCTA 
CTAGGAGGCT ATGGTGCAAC CCTCAGTGTT CTAGATCAGA AGATATTGCT GCTTCTTAGA 
TTGTATGAAA GAAATGATCT GAGCCTCATA AACTTCAGAA TGCTGCTATG GGGCCCTGCT 
GCTGTGGAAC ATCACAAGAC GTGTAAGAGC CTGGGAAAGT CACTATGGCA GCAGCCAAGT 
GTGGGAGAAA TCTTATGTCT CCTGGATCGA GAGAGAATGA TGAAGACCAT TCTTCACTTC 
CCACAGAATC GAAAACTTCT GCTAAAGGAG GATGCACAGG ATATATTCAA AGACAAAAGC 
AAAGTAGACT TCGACGAACT TTATGATCCC TGTTTTCTAC TTCACCTTTT CAGTGAACTT 
ACCAGACCAG AATTTATAGT GGATTGTCAC AAATTTGTTG ATTCCAATGC CCTGGGCCTC 
ACAGTTTCTG CCCTTAGCAG TTACGATCCT CAGATGCGAG CAGCAGCTTA TTATGTTCTG 
GCTTCCTATT ATTCCCATTT GGAAGGGGCT CGATTTCGAG AACAGCCACA GCTGCTCTAT 
CTCTTGGACA TATTACAAAA TGGAATCAGA AAACAGAATC TCAGATTGAC CTATACCTTG 
ACACTGTTTA TTGCCAAAAC TGCCTTGCAG ATTCTGAAAC CAGAGGAGCA CATGTACATG 
AAGATAAACA AATTCCTTTT GTCACATCAG GACTTATACT TGAACAAAGT ACCGGGATTC 
TATAACTTTT TCTACAGCTC TGATTCAGAG TATAGGATAG AACAGGAGTG GATATTTGAA 
CTTTTGAGAC AAGGAATTCG AGACACACAC TGCTATGATT TGTATTCCTC TCAGAGAATT 
TTCCATATCA TCCTGTCCTT CTTCAACAGC CCATTATGTG ATGAAACAAT GCAGAACTGG 
ATTATAGAAA TCTTACAAAA TGCTGCCCAG ATTACCAAAG CTGCCTTTGA AATTGTGCAG 
GAACATAGCC TTTTAACTTG GATTTTGCAT ATCCTTGGGA AAAAGTTTTT GGAAAACCAG 
ATGCTATCCA ACATTATATG CTTGCTGCAT ACTCTCTGGA TATCCAACTT GGGAAACAAA 
AGAATGGAAA TACAGGAAGA CCATCCTTGC GAGCTTGTGG CTACACAGTC ACCAAAGCTG 
CTTCCTCTAC AATTTGTCAA TGAATTCCTC TATGTTTTGG TTGTGTTGGC GAAATATCTT 
CGGCCCACCT TGGACTCTAT CCAGATCACT CACTATTTTA GTACTTTGAG CTCTGTGCTG 
AGGTACCGAG AAACTGTTTT AGAAGCCTTC AAGGAAATGG ACCGATTGAC TGTAAATGAA 
AATGTATTTT CTACCAAAGA TGTCCTCATG CTCTTACATA AGTGGAGCAT AATTATGGGA 
GATGTTAAAC TCCAAGAAGA CTTGAAAACA GTGATTGAAA AATACCAAGT CAAGGAATTA 
ATGAAAATTA TTAAAGATAG GAACAAACCC CTTGCACCAG CTCGATCTAA AGCTCAGCGT 
AACAAAAAAA AGATGGAGGT GGATCCAGAG GAAACAATTG ATCCAGAGTT AAAGGCTTCC 
AACCTGATGA AATGCCGAGG TCTCCTGAGA TCCATACTGA CCCACTGGGA CCCCGCCTTT 
GCTGTGGTAG AGACAGAGCC AAAGCAAGAA CGAGTCTCCT TAGCTCCTGA CCCAGAGAGA 
GAAGCTGTGG ATCTTGTTGG GGCCACCGCT GTTCTGGTGG CCCAATGGGA GATCAGCTCC 
CTCATGGACC CAGCGCTGGA TGTCCAGGGA ATTCCAGAGG TCCTCAGGTG GTTTAAAAGC 
AGCATTTTGC TTCATCCTGT GGTTGTCTCA GAACTTGTCA GAGATGTTAC ATGGAGAAAT 
AGCATCTTTA AGCTGTACAG CCAGCTTTCC CATGCCAGAG AACTGAAAGC AGCCTCTCAG 
AGCGTCTTCT GCCTCTTCAA TACAGTCATG CTTCACTTAA TGGCATCCCA GGGCCCAATA 
GAGAACTCTT TTCATGAAGT GGTAGAAACA ATCGCTCTTT CTTCTACAGA TGAAGAAGAC 
AAAACAAAAC AAGCTGCTGC AGTGTTCCTG GTATCTCTTT ATATTAAAGA TATCTGGCTA 
GGGGCAAAGC CAGTGAATAC CTTCCTGACT CACATCCGAA TGATCTGTGA TGCCACAGAG 
TTGTTCAGTG AGGATGAAGA GAAGGCTCGG GAACAAGAAG AAGCAATAGT AGCACTGTGT 
GAGAATATCA CTGTTGCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023354673.1
CDS	18..5681
Translation	MPRGLQSLNKISVAIYEAQPEISPAFKTKEFIPLSRLLSMVMVTTVPNVCNKIMFTQGLKLENKSVNHTILSLISTILKRALKNIDHCLNKDIWQESSIYTPVMMEEFVHLYREAVSKLLPDLNNIVCVWQSLGKQKIDEDGGLEKKNSLEDEKTETTEVDHKHDDAETTLLKAILLQVICLYQMVVPHVVIQCTFDFSKLLKGIISEEGFQEEVPPVLQYYILKVALELPASKFAWLKGQESPNSGERSVFYLLMKMFVTSKHSQLKASTKLLITKILRDTGVFEYTWKELELWVEQLDNTAENRKEVVIQFLECVLLQLVTNPYSYTDKAADFVQEASTLQATVVKQDADNVSIPVSHIDDILDMVDVLMEDTEGLDEEVGFSLNEDMILLTFPFSAIVPTALESRNKLLLGNGNDSGESIVEYLIAVLTDLLHTQRDPLALCLMLQSYDSLQLSNTSLYTRIYQFNRYYNLWIPEQARETLEFQATRPTETSLVVGSAFSTLLTDTYERGVTLLLEENIQGKLREKIAQLPMQKVLLSVKQVMLYLRTTVENFAKLGKSSGPALLQLFMNLLSHLLNHCYHLDTCNQKKCEAAQAESAIFLDEESLATLELANDKTLEDALIVIFRHPTLESWYLALELQTLPSHNLNPILVKLLATHFNAGVLSLLKLSAPILQEMQRMDILSKYLEAITKSVLKELHTLKKTDPPIAYGKPSAQLEALYQLHPYMDGTQLRDITLALLALPKSTLLDQESKKSHQKKKHLSFLGETLKQLITESYQEQSQQGELLSSLEYVKRMSVLLPALVIEELETVFFQALQKEPILVHVVELDLLTYCLNRRTKTSLSIVALLIQQSSTHLLHFELWCLQGLGSCLPENFSLFLPLIHTYLQSCMQGDFTRPSKVSRNVMNVLRKVLWKELQNSLLGDDPQDLGIQEEVLSKLIPYSRAKELRLLIDKMPSILQKPEKSKSWIVSDAIALVLEKSVEELDVWKKTLLGSCIKWLIASYSSSIKEENTQDAEKSMLLRFNELLNSVNEVDPVDWQKFVKTGLKFRYHDYTFLKTLHACILLLYQPENSLCKNLVQLPLIYVMITQHSMFLPTILQTKEDDTNIQVKEMLVDLISTIVKLCPSVCESNHFAVLLGGYGATLSVLDQKILLLLRLYERNDLSLINFRMLLWGPAAVEHHKTCKSLGKSLWQQPSVGEILCLLDRERMMKTILHFPQNRKLLLKEDAQDIFKDKSKVDFDELYDPCFLLHLFSELTRPEFIVDCHKFVDSNALGLTVSALSSYDPQMRAAAYYVLASYYSHLEGARFREQPQLLYLLDILQNGIRKQNLRLTYTLTLFIAKTALQILKPEEHMYMKINKFLLSHQDLYLNKVPGFYNFFYSSDSEYRIEQEWIFELLRQGIRDTHCYDLYSSQRIFHIILSFFNSPLCDETMQNWIIEILQNAAQITKAAFEIVQEHSLLTWILHILGKKFLENQMLSNIICLLHTLWISNLGNKRMEIQEDHPCELVATQSPKLLPLQFVNEFLYVLVVLAKYLRPTLDSIQITHYFSTLSSVLRYRETVLEAFKEMDRLTVNENVFSTKDVLMLLHKWSIIMGDVKLQEDLKTVIEKYQVKELMKIIKDRNKPLAPARSKAQRNKKKMEVDPEETIDPELKASNLMKCRGLLRSILTHWDPAFAVVETEPKQERVSLAPDPEREAVDLVGATAVLVAQWEISSLMDPALDVQGIPEVLRWFKSSILLHPVVVSELVRDVTWRNSIFKLYSQLSHARELKAASQSVFCLFNTVMLHLMASQGPIENSFHEVVETIALSSTDEEDKTKQAAAVFLVSLYIKDIWLGAKPVNTFLTHIRMICDATELFSEDEEKAREQEEAIVALCENITVAT

Target ORF information:

RefSeq Version	XM_023498905.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii URB1 ribosome biogenesis 1 homolog (S. cerevisiae) (URB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023498905.1

ORF Insert Sequence:

ATGCCGAGAG GCCTGCAATC ACTTAACAAA ATTTCAGTGG CTATTTATGA GGCTCAGCCA 
GAGATTTCCC CAGCATTTAA AACTAAAGAG TTTATACCCT TGTCTAGATT GCTTTCCATG 
GTCATGGTGA CAACAGTACC CAACGTGTGC AACAAAATCA TGTTTACTCA AGGATTAAAA 
TTGGAAAACA AATCAGTAAA TCATACAATT TTATCACTTA TTTCCACCAT TTTGAAGAGA 
GCATTAAAAA ACATTGATCA CTGTCTCAAT AAGGATATAT GGCAGGAGTC AAGCATCTAT 
ACTCCTGTGA TGATGGAGGA ATTTGTGCAT CTGTACAGAG AAGCTGTAAG CAAGCTTTTG 
CCAGATTTGA ACAATATTGT ATGTGTGTGG CAATCACTTG GAAAGCAAAA AATAGATGAA 
GATGGCGGGT TAGAAAAAAA AAATTCCCTT GAAGATGAGA AAACTGAAAC TACAGAAGTA 
GACCACAAAC ATGATGATGC AGAAACAACT CTTTTGAAGG CTATTTTACT TCAGGTCATT 
TGTCTTTATC AGATGGTTGT TCCCCATGTT GTCATTCAAT GTACATTTGA CTTCAGCAAG 
CTTCTAAAAG GTATCATTTC AGAAGAGGGA TTTCAAGAGG AAGTTCCTCC TGTCCTTCAG 
TACTATATTC TGAAAGTGGC CCTAGAATTG CCTGCCAGCA AATTTGCCTG GTTAAAAGGA 
CAGGAAAGTC CAAATAGTGG AGAAAGATCT GTGTTTTATT TGCTGATGAA AATGTTTGTG 
ACAAGTAAAC ACTCACAACT TAAAGCATCA ACAAAACTAT TGATTACAAA GATTTTACGT 
GACACTGGAG TATTTGAATA TACATGGAAA GAGCTAGAAC TATGGGTGGA GCAATTAGAT 
AACACAGCAG AAAACAGAAA AGAAGTTGTG ATTCAGTTTT TGGAATGTGT TTTGTTGCAA 
TTGGTAACAA ATCCCTATTC ATATACAGAC AAGGCAGCAG ATTTTGTTCA AGAAGCTAGT 
ACACTACAGG CCACTGTGGT GAAGCAAGAT GCTGATAATG TTAGCATACC TGTATCACAC 
ATTGATGATA TCCTGGATAT GGTGGATGTC CTCATGGAGG ACACTGAAGG CTTAGATGAG 
GAAGTTGGTT TCTCTTTAAA TGAAGATATG ATTCTGCTGA CATTCCCATT CAGCGCCATA 
GTTCCTACAG CTCTGGAGAG CAGGAATAAA TTGCTTCTTG GGAATGGAAA TGATTCAGGT 
GAAAGCATAG TGGAATATCT GATAGCCGTT CTGACAGACC TTCTACATAC ACAGCGAGAT 
CCTTTGGCTC TTTGTCTGAT GCTTCAGTCT TATGACAGTT TACAACTTTC AAACACATCT 
TTGTATACTC GGATTTACCA GTTCAACAGA TACTATAACC TGTGGATACC TGAGCAAGCC 
AGAGAAACTT TGGAATTTCA AGCTACCAGA CCCACTGAGA CATCACTTGT GGTTGGCTCT 
GCCTTTTCCA CCTTGTTAAC AGATACCTAT GAGAGGGGAG TTACCCTCCT TTTAGAAGAG 
AATATCCAGG GAAAACTGAG AGAAAAAATT GCTCAGCTCC CAATGCAGAA GGTTCTGCTC 
TCTGTGAAGC AGGTGATGCT GTATCTTAGA ACCACTGTGG AGAACTTTGC CAAGCTTGGT 
AAAAGTTCAG GTCCTGCTTT GCTTCAGCTC TTCATGAATT TGCTCAGTCA CCTGTTGAAC 
CATTGTTATC ATTTGGACAC ATGTAACCAG AAAAAATGTG AAGCTGCTCA GGCCGAATCT 
GCTATCTTTT TGGATGAGGA GTCCTTGGCC ACACTGGAAT TAGCCAATGA TAAGACTCTG 
GAAGATGCGT TGATAGTAAT CTTTAGACAT CCCACATTGG AGAGCTGGTA TTTGGCCCTG 
GAGCTGCAGA CTCTTCCCTC ACATAACCTC AATCCAATCC TTGTAAAGCT TTTAGCAACT 
CATTTCAATG CTGGGGTCCT CTCACTCCTG AAACTGAGTG CCCCAATTCT GCAGGAAATG 
CAACGTATGG ACATCCTTTC CAAGTATTTA GAAGCCATTA CTAAGAGTGT CTTGAAAGAA 
CTACACACCC TGAAGAAGAC AGATCCTCCC ATTGCATATG GGAAACCTTC AGCTCAGCTG 
GAAGCTTTGT ACCAACTACA TCCCTACATG GATGGAACCC AGCTTAGGGA CATTACGCTT 
GCACTCCTGG CTCTTCCTAA ATCAACCCTT TTGGATCAGG AATCTAAGAA ATCTCACCAA 
AAAAAAAAAC ATCTGAGCTT TTTAGGGGAA ACCCTCAAAC AGCTTATCAC TGAATCCTAC 
CAGGAACAAT CCCAGCAAGG TGAATTACTG TCGTCCCTAG AATATGTGAA GAGAATGAGT 
GTCCTGTTGC CTGCACTGGT CATTGAAGAG TTAGAGACTG TGTTCTTCCA AGCTTTACAG 
AAAGAGCCAA TTCTGGTCCA TGTAGTTGAG TTAGATTTAT TGACCTACTG TCTAAATCGG 
AGGACAAAAA CTTCTCTTTC CATTGTAGCT TTGTTGATTC AGCAAAGTAG TACTCACCTG 
CTACACTTTG AGTTGTGGTG CTTACAGGGC CTAGGGTCCT GCCTTCCAGA AAACTTCAGC 
CTCTTTCTTC CACTTATCCA TACCTATTTA CAGTCTTGTA TGCAGGGAGA CTTTACACGT 
CCTTCTAAAG TGTCTAGAAA TGTCATGAAT GTGTTGAGAA AGGTCCTGTG GAAAGAGCTT 
CAAAACAGTC TTTTAGGAGA TGATCCTCAG GATCTTGGAA TTCAAGAAGA GGTCCTTTCT 
AAATTGATTC CCTATTCAAG AGCAAAAGAA CTTAGACTCC TCATTGACAA AATGCCCAGC 
ATTCTTCAAA AGCCAGAGAA ATCGAAGAGC TGGATTGTAT CAGATGCCAT AGCATTAGTA 
TTGGAAAAAT CTGTGGAAGA GCTGGATGTT TGGAAAAAGA CTTTGTTGGG GTCATGTATA 
AAATGGCTAA TAGCTTCATA CAGCAGTAGT ATCAAAGAAG AAAATACCCA GGATGCAGAG 
AAGTCTATGT TGTTGCGATT TAATGAATTA TTGAACTCAG TTAATGAAGT TGATCCTGTT 
GATTGGCAGA AGTTTGTGAA GACAGGCCTT AAGTTCCGCT ACCATGATTA CACATTTTTA 
AAGACTCTTC ATGCTTGTAT ACTGCTGTTA TATCAGCCTG AAAATTCCCT TTGCAAAAAC 
TTAGTGCAGC TGCCACTAAT ATATGTGATG ATCACACAAC ACTCTATGTT TTTGCCAACC 
ATATTGCAAA CAAAAGAAGA TGACACAAAT ATCCAAGTAA AAGAAATGTT AGTGGACCTA 
ATATCAACTA TTGTAAAACT TTGCCCTTCC GTCTGTGAAA GCAACCACTT TGCAGTGCTA 
CTAGGAGGCT ATGGTGCAAC CCTCAGTGTT CTAGATCAGA AGATATTGCT GCTTCTTAGA 
TTGTATGAAA GAAATGATCT GAGCCTCATA AACTTCAGAA TGCTGCTATG GGGCCCTGCT 
GCTGTGGAAC ATCACAAGAC GTGTAAGAGC CTGGGAAAGT CACTATGGCA GCAGCCAAGT 
GTGGGAGAAA TCTTATGTCT CCTGGATCGA GAGAGAATGA TGAAGACCAT TCTTCACTTC 
CCACAGAATC GAAAACTTCT GCTAAAGGAG GATGCACAGG ATATATTCAA AGACAAAAGC 
AAAGTAGACT TCGACGAACT TTATGATCCC TGTTTTCTAC TTCACCTTTT CAGTGAACTT 
ACCAGACCAG AATTTATAGT GGATTGTCAC AAATTTGTTG ATTCCAATGC CCTGGGCCTC 
ACAGTTTCTG CCCTTAGCAG TTACGATCCT CAGATGCGAG CAGCAGCTTA TTATGTTCTG 
GCTTCCTATT ATTCCCATTT GGAAGGGGCT CGATTTCGAG AACAGCCACA GCTGCTCTAT 
CTCTTGGACA TATTACAAAA TGGAATCAGA AAACAGAATC TCAGATTGAC CTATACCTTG 
ACACTGTTTA TTGCCAAAAC TGCCTTGCAG ATTCTGAAAC CAGAGGAGCA CATGTACATG 
AAGATAAACA AATTCCTTTT GTCACATCAG GACTTATACT TGAACAAAGT ACCGGGATTC 
TATAACTTTT TCTACAGCTC TGATTCAGAG TATAGGATAG AACAGGAGTG GATATTTGAA 
CTTTTGAGAC AAGGAATTCG AGACACACAC TGCTATGATT TGTATTCCTC TCAGAGAATT 
TTCCATATCA TCCTGTCCTT CTTCAACAGC CCATTATGTG ATGAAACAAT GCAGAACTGG 
ATTATAGAAA TCTTACAAAA TGCTGCCCAG ATTACCAAAG CTGCCTTTGA AATTGTGCAG 
GAACATAGCC TTTTAACTTG GATTTTGCAT ATCCTTGGGA AAAAGTTTTT GGAAAACCAG 
ATGCTATCCA ACATTATATG CTTGCTGCAT ACTCTCTGGA TATCCAACTT GGGAAACAAA 
AGAATGGAAA TACAGGAAGA CCATCCTTGC GAGCTTGTGG CTACACAGTC ACCAAAGCTG 
CTTCCTCTAC AATTTGTCAA TGAATTCCTC TATGTTTTGG TTGTGTTGGC GAAATATCTT 
CGGCCCACCT TGGACTCTAT CCAGATCACT CACTATTTTA GTACTTTGAG CTCTGTGCTG 
AGGTACCGAG AAACTGTTTT AGAAGCCTTC AAGGAAATGG ACCGATTGAC TGTAAATGAA 
AATGTATTTT CTACCAAAGA TGTCCTCATG CTCTTACATA AGTGGAGCAT AATTATGGGA 
GATGTTAAAC TCCAAGAAGA CTTGAAAACA GTGATTGAAA AATACCAAGT CAAGGAATTA 
ATGAAAATTA TTAAAGATAG GAACAAACCC CTTGCACCAG CTCGATCTAA AGCTCAGCGT 
AACAAAAAAA AGATGGAGGT GGATCCAGAG GAAACAATTG ATCCAGAGTT AAAGGCTTCC 
AACCTGATGA AATGCCGAGG TCTCCTGAGA TCCATACTGA CCCACTGGGA CCCCGCCTTT 
GCTGTGGTAG AGACAGAGCC AAAGCAAGAA CGAGTCTCCT TAGCTCCTGA CCCAGAGAGA 
GAAGCTGTGG ATCTTGTTGG GGCCACCGCT GTTCTGGTGG CCCAATGGGA GATCAGCTCC 
CTCATGGACC CAGCGCTGGA TGTCCAGGGA ATTCCAGAGG TCCTCAGGTG GTTTAAAAGC 
AGCATTTTGC TTCATCCTGT GGTTGTCTCA GAACTTGTCA GAGATGTTAC ATGGAGAAAT 
AGCATCTTTA AGCTGTACAG CCAGCTTTCC CATGCCAGAG AACTGAAAGC AGCCTCTCAG 
AGCGTCTTCT GCCTCTTCAA TACAGTCATG CTTCACTTAA TGGCATCCCA GGGCCCAATA 
GAGAACTCTT TTCATGAAGT GGTAGAAACA ATCGCTCTTT CTTCTACAGA TGAAGAAGAC 
AAAACAAAAC AAGCTGCTGC AGTGTTCCTG GTATCTCTTT ATATTAAAGA TATCTGGCTA 
GGGGCAAAGC CAGTGAATAC CTTCCTGACT CACATCCGAA TGATCTGTGA TGCCACAGAG 
TTGTTCAGTG AGGATGAAGA GAAGGCTCGG GAACAAGAAG AAGCAATAGT AGCACTGTGT 
GAGAATATCA CTGTTGCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

URB1 ( XM_012544405.3 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012399859.1 Sarcophilus harrisii URB1 ribosome biogenesis homolog (URB1), transcript variant X1, mRNA.

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CloneID	OSa125228
Clone ID Related Accession (Same CDS sequence)	XM_012544405.3 , XM_012544405.2 , XM_012544405.1
Accession Version	XM_012544405.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	nucleolar pre-ribosomal-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_012544405.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACC CAAAGAGGAA AGCCCTTAAG GAAGAGAGCA AGGCCTCCTC GGCCGGTCCT 
GCGAAAAGGC CTCGCGGGGA AGATTTCACC GGGCAGCAGT TTAAGACACA GCTCAAAGAC 
CCTCAGACTA TGGTGTCAGC TTTGGAAACT TTTATCTCCA TTGCCAAAAT GTTACCACAA 
ACTGGACTAT ATGATGTCGT GGAAGGATAT ATAAAAATTT CTGTTGAGTG CTCAGAGATT 
TTCCAACTTT TAAATGGAGA AAAAAGAACT GAACATGAAA GGATATTAAT TTTTAAAACT 
TTAGAGGCCA TATTACTGCG AACAGCAGGT GATCTTTCTC ATTTTCATGT TGTGGGAACC 
AACATCATGA AAAAAGTTAT GACTAGCCAT ATGAAGCTGA TTTATGATTC ACTAAATGCT 
TCAAGCTACA AAATGAGTCA AACCTGTTTG AACCTTATGT CCGCTATGGT GGCACAGGGT 
CCAGATGCTG CCAGGGATTT CTATAGCCGA TTTGACTTCA ACAAAAATTC ATTGTATGGT 
TTGGTGAAAA TAAGAAGTCC AAAGGGGCTG CCAGATGTTC GATTGGCATA TATCCAGTTT 
GCCCTTTCCT TCTTAATTGC TGGTGATGAT AATACCATAG CACGAGTGTT GGAGTTGAAA 
GAATTCCTCC CTGTCATTTT TGCGACAGGC TTGAAGGAAG ACAGAATTTC TACTATCAAT 
CTTTTGTTGT CCACCCTGAA AACCAAGGTG GTTCACAATA TTGGTGTTAC AAAGACTCAG 
AAAGTTCGTT TCTTCACTGG ATATTTGTTA AATAATGTGG CTTCTCTCTA TCGTTGGAAT 
GGAATTGCTG ATGTCAGCGC AGAAAATATC AAGCCTACTG AAGAAGCAGG CAAGACCATG 
GTAAGGGAAC TGGTTCACAA CTTCTTAATG GATCTTTGCT GTTCTCTGAA GCATGGCATT 
AACTTCTATG ATGCATCTTT TGGTACTCTG GGCAGAGGAG GGAACTTGAC TCTCTTGAAC 
TTCTTACTGG GTTTGAAGAC AGCAGCAGAT GATGACTTGG TGGCTGAACT TGTAGTGAAC 
ATCCTAAAAG TATGCCCAGA CTTACTTACG AAGTACTTTA AGGAGGTGAC CTATTCCTTT 
GTTCCAAGGA TGAAGTCGGC TTGGTTAAAC AATATCAGGC TACTAAGGAA GATTTATGAG 
GCTCAGCCAG AGATTTCCCC AGCATTTAAA ACTAAAGAGT TTATACCCTT GTCTAGATTG 
CTTTCCATGG TCATGGTGAC AACAGTACCC AACGTGTGCA ACAAAATCAT GTTTACTCAA 
GGATTAAAAT TGGAAAACAA ATCAGTAAAT CATACAATTT TATCACTTAT TTCCACCATT 
TTGAAGAGAG CATTAAAAAA CATTGATCAC TGTCTCAATA AGGATATATG GCAGGAGTCA 
AGCATCTATA CTCCTGTGAT GATGGAGGAA TTTGTGCATC TGTACAGAGA AGCTGTAAGC 
AAGCTTTTGC CAGATTTGAA CAATATTGTA TGTGTGTGGC AATCACTTGG AAAGCAAAAA 
ATAGATGAAG ATGGCGGGTT AGAAAAAAAA AATTCCCTTG AAGATGAGAA AACTGAAACT 
ACAGAAGTAG ACCACAAACA TGATGATGCA GAAACAACTC TTTTGAAGGC TATTTTACTT 
CAGGTCATTT GTCTTTATCA GATGGTTGTT CCCCATGTTG TCATTCAATG TACATTTGAC 
TTCAGCAAGC TTCTAAAAGG TATCATTTCA GAAGAGGGAT TTCAAGAGGA AGTTCCTCCT 
GTCCTTCAGT ACTATATTCT GAAAGTGGCC CTAGAATTGC CTGCCAGCAA ATTTGCCTGG 
TTAAAAGGAC AGGAAAGTCC AAATAGTGGA GAAAGATCTG TGTTTTATTT GCTGATGAAA 
ATGTTTGTGA CAAGTAAACA CTCACAACTT AAAGCATCAA CAAAACTATT GATTACAAAG 
ATTTTACGTG ACACTGGAGT ATTTGAATAT ACATGGAAAG AGCTAGAACT ATGGGTGGAG 
CAATTAGATA ACACAGCAGA AAACAGAAAA GAAGTTGTGA TTCAGTTTTT GGAATGTGTT 
TTGTTGCAAT TGGTAACAAA TCCCTATTCA TATACAGACA AGGCAGCAGA TTTTGTTCAA 
GAAGCTAGTA CACTACAGGC CACTGTGGTG AAGCAAGATG CTGATAATGT TAGCATACCT 
GTATCACACA TTGATGATAT CCTGGATATG GTGGATGTCC TCATGGAGGA CACTGAAGGC 
TTAGATGAGG AAGTTGGTTT CTCTTTAAAT GAAGATATGA TTCTGCTGAC ATTCCCATTC 
AGCGCCATAG TTCCTACAGC TCTGGAGAGC AGGAATAAAT TGCTTCTTGG GAATGGAAAT 
GATTCAGGTG AAAGCATAGT GGAATATCTG ATAGCCGTTC TGACAGACCT TCTACATACA 
CAGCGAGATC CTTTGGCTCT TTGTCTGATG CTTCAGTCTT ATGACAGTTT ACAACTTTCA 
AACACATCTT TGTATACTCG GATTTACCAG TTCAACAGAT ACTATAACCT GTGGATACCT 
GAGCAAGCCA GAGAAACTTT GGAATTTCAA GCTACCAGAC CCACTGAGAC ATCACTTGTG 
GTTGGCTCTG CCTTTTCCAC CTTGTTAACA GATACCTATG AGAGGGGAGT TACCCTCCTT 
TTAGAAGAGA ATATCCAGGG AAAACTGAGA GAAAAAATTG CTCAGCTCCC AATGCAGAAG 
GTTCTGCTCT CTGTGAAGCA GGTGATGCTG TATCTTAGAA CCACTGTGGA GAACTTTGCC 
AAGCTTGGTA AAAGTTCAGG TCCTGCTTTG CTTCAGCTCT TCATGAATTT GCTCAGTCAC 
CTGTTGAACC ATTGTTATCA TTTGGACACA TGTAACCAGA AAAAATGTGA AGCTGCTCAG 
GCCGAATCTG CTATCTTTTT GGATGAGGAG TCCTTGGCCA CACTGGAATT AGCCAATGAT 
AAGACTCTGG AAGATGCGTT GATAGTAATC TTTAGACATC CCACATTGGA GAGCTGGTAT 
TTGGCCCTGG AGCTGCAGAC TCTTCCCTCA CATAACCTCA ATCCAATCCT TGTAAAGCTT 
TTAGCAACTC ATTTCAATGC TGGGGTCCTC TCACTCCTGA AACTGAGTGC CCCAATTCTG 
CAGGAAATGC AACGTATGGA CATCCTTTCC AAGTATTTAG AAGCCATTAC TAAGAGTGTC 
TTGAAAGAAC TACACACCCT GAAGAAGACA GATCCTCCCA TTGCATATGG GAAACCTTCA 
GCTCAGCTGG AAGCTTTGTA CCAACTACAT CCCTACATGG ATGGAACCCA GCTTAGGGAC 
ATTACGCTTG CACTCCTGGC TCTTCCTAAA TCAACCCTTT TGGATCAGGA ATCTAAGAAA 
TCTCACCAAA AAAAAAAACA TCTGAGCTTT TTAGGGGAAA CCCTCAAACA GCTTATCACT 
GAATCCTACC AGGAACAATC CCAGCAAGGT GAATTACTGT CGTCCCTAGA ATATGTGAAG 
AGAATGAGTG TCCTGTTGCC TGCACTGGTC ATTGAAGAGT TAGAGACTGT GTTCTTCCAA 
GCTTTACAGA AAGAGCCAAT TCTGGTCCAT GTAGTTGAGT TAGATTTATT GACCTACTGT 
CTAAATCGGA GGACAAAAAC TTCTCTTTCC ATTGTAGCTT TGTTGATTCA GCAAAGTAGT 
ACTCACCTGC TACACTTTGA GTTGTGGTGC TTACAGGGCC TAGGGTCCTG CCTTCCAGAA 
AACTTCAGCC TCTTTCTTCC ACTTATCCAT ACCTATTTAC AGTCTTGTAT GCAGGGAGAC 
TTTACACGTC CTTCTAAAGT GTCTAGAAAT GTCATGAATG TGTTGAGAAA GGTCCTGTGG 
AAAGAGCTTC AAAACAGTCT TTTAGGAGAT GATCCTCAGG ATCTTGGAAT TCAAGAAGAG 
GTCCTTTCTA AATTGATTCC CTATTCAAGA GCAAAAGAAC TTAGACTCCT CATTGACAAA 
ATGCCCAGCA TTCTTCAAAA GCCAGAGAAA TCGAAGAGCT GGATTGTATC AGATGCCATA 
GCATTAGTAT TGGAAAAATC TGTGGAAGAG CTGGATGTTT GGAAAAAGAC TTTGTTGGGG 
TCATGTATAA AATGGCTAAT AGCTTCATAC AGCAGTAGTA TCAAAGAAGA AAATACCCAG 
GATGCAGAGA AGTCTATGTT GTTGCGATTT AATGAATTAT TGAACTCAGT TAATGAAGTT 
GATCCTGTTG ATTGGCAGAA GTTTGTGAAG ACAGGCCTTA AGTTCCGCTA CCATGATTAC 
ACATTTTTAA AGACTCTTCA TGCTTGTATA CTGCTGTTAT ATCAGCCTGA AAATTCCCTT 
TGCAAAAACT TAGTGCAGCT GCCACTAATA TATGTGATGA TCACACAACA CTCTATGTTT 
TTGCCAACCA TATTGCAAAC AAAAGAAGAT GACACAAATA TCCAAGTAAA AGAAATGTTA 
GTGGACCTAA TATCAACTAT TGTAAAACTT TGCCCTTCCG TCTGTGAAAG CAACCACTTT 
GCAGTGCTAC TAGGAGGCTA TGGTGCAACC CTCAGTGTTC TAGATCAGAA GATATTGCTG 
CTTCTTAGAT TGTATGAAAG AAATGATCTG AGCCTCATAA ACTTCAGAAT GCTGCTATGG 
GGCCCTGCTG CTGTGGAACA TCACAAGACG TGTAAGAGCC TGGGAAAGTC ACTATGGCAG 
CAGCCAAGTG TGGGAGAAAT CTTATGTCTC CTGGATCGAG AGAGAATGAT GAAGACCATT 
CTTCACTTCC CACAGAATCG AAAACTTCTG CTAAAGGAGG ATGCACAGGA TATATTCAAA 
GACAAAAGCA AAGTAGACTT CGACGAACTT TATGATCCCT GTTTTCTACT TCACCTTTTC 
AGTGAACTTA CCAGACCAGA ATTTATAGTG GATTGTCACA AATTTGTTGA TTCCAATGCC 
CTGGGCCTCA CAGTTTCTGC CCTTAGCAGT TACGATCCTC AGATGCGAGC AGCAGCTTAT 
TATGTTCTGG CTTCCTATTA TTCCCATTTG GAAGGGGCTC GATTTCGAGA ACAGCCACAG 
CTGCTCTATC TCTTGGACAT ATTACAAAAT GGAATCAGAA AACAGAATCT CAGATTGACC 
TATACCTTGA CACTGTTTAT TGCCAAAACT GCCTTGCAGA TTCTGAAACC AGAGGAGCAC 
ATGTACATGA AGATAAACAA ATTCCTTTTG TCACATCAGG ACTTATACTT GAACAAAGTA 
CCGGGATTCT ATAACTTTTT CTACAGCTCT GATTCAGAGT ATAGGATAGA ACAGGAGTGG 
ATATTTGAAC TTTTGAGACA AGGAATTCGA GACACACACT GCTATGATTT GTATTCCTCT 
CAGAGAATTT TCCATATCAT CCTGTCCTTC TTCAACAGCC CATTATGTGA TGAAACAATG 
CAGAACTGGA TTATAGAAAT CTTACAAAAT GCTGCCCAGA TTACCAAAGC TGCCTTTGAA 
ATTGTGCAGG AACATAGCCT TTTAACTTGG ATTTTGCATA TCCTTGGGAA AAAGTTTTTG 
GAAAACCAGA TGCTATCCAA CATTATATGC TTGCTGCATA CTCTCTGGAT ATCCAACTTG 
GGAAACAAAA GAATGGAAAT ACAGGAAGAC CATCCTTGCG AGCTTGTGGC TACACAGTCA 
CCAAAGCTGC TTCCTCTACA ATTTGTCAAT GAATTCCTCT ATGTTTTGGT TGTGTTGGCG 
AAATATCTTC GGCCCACCTT GGACTCTATC CAGATCACTC ACTATTTTAG TACTTTGAGC 
TCTGTGCTGA GGTACCGAGA AACTGTTTTA GAAGCCTTCA AGGAAATGGA CCGATTGACT 
GTAAATGAAA ATGTATTTTC TACCAAAGAT GTCCTCATGC TCTTACATAA GTGGAGCATA 
ATTATGGGAG ATGTTAAACT CCAAGAAGAC TTGAAAACAG TGATTGAAAA ATACCAAGTC 
AAGGAATTAA TGAAAATTAT TAAAGATAGG AACAAACCCC TTGCACCAGC TCGATCTAAA 
GCTCAGCGTA ACAAAAAAAA GATGGAGGTG GATCCAGAGG AAACAATTGA TCCAGAGTTA 
AAGGCTTCCA ACCTGATGAA ATGCCGAGGT CTCCTGAGAT CCATACTGAC CCACTGGGAC 
CCCGCCTTTG CTGTGGTAGA GACAGAGCCA AAGCAAGAAC GAGTCTCCTT AGCTCCTGAC 
CCAGAGAGAG AAGCTGTGGA TCTTGTTGGG GCCACCGCTG TTCTGGTGGC CCAATGGGAG 
ATCAGCTCCC TCATGGACCC AGCGCTGGAT GTCCAGGGAA TTCCAGAGGT CCTCAGGTGG 
TTTAAAAGCA GCATTTTGCT TCATCCTGTG GTTGTCTCAG AACTTGTCAG AGATGTTACA 
TGGAGAAATA GCATCTTTAA GCTGTACAGC CAGCTTTCCC ATGCCAGAGA ACTGAAAGCA 
GCCTCTCAGA GCGTCTTCTG CCTCTTCAAT ACAGTCATGC TTCACTTAAT GGCATCCCAG 
GGCCCAATAG AGAACTCTTT TCATGAAGTG GTAGAAACAA TCGCTCTTTC TTCTACAGAT 
GAAGAAGACA AAACAAAACA AGCTGCTGCA GTGTTCCTGG TATCTCTTTA TATTAAAGAT 
ATCTGGCTAG GGGCAAAGCC AGTGAATACC TTCCTGACTC ACATCCGAAT GATCTGTGAT 
GCCACAGAGT TGTTCAGTGA GGATGAAGAG AAGGCTCGGG AACAAGAAGA AGCAATAGTA 
GCACTGTGTG AGAATATCAC TGTTGCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012399859.1
CDS	207..7019
Translation	MGNPKRKALKEESKASSAGPAKRPRGEDFTGQQFKTQLKDPQTMVSALETFISIAKMLPQTGLYDVVEGYIKISVECSEIFQLLNGEKRTEHERILIFKTLEAILLRTAGDLSHFHVVGTNIMKKVMTSHMKLIYDSLNASSYKMSQTCLNLMSAMVAQGPDAARDFYSRFDFNKNSLYGLVKIRSPKGLPDVRLAYIQFALSFLIAGDDNTIARVLELKEFLPVIFATGLKEDRISTINLLLSTLKTKVVHNIGVTKTQKVRFFTGYLLNNVASLYRWNGIADVSAENIKPTEEAGKTMVRELVHNFLMDLCCSLKHGINFYDASFGTLGRGGNLTLLNFLLGLKTAADDDLVAELVVNILKVCPDLLTKYFKEVTYSFVPRMKSAWLNNIRLLRKIYEAQPEISPAFKTKEFIPLSRLLSMVMVTTVPNVCNKIMFTQGLKLENKSVNHTILSLISTILKRALKNIDHCLNKDIWQESSIYTPVMMEEFVHLYREAVSKLLPDLNNIVCVWQSLGKQKIDEDGGLEKKNSLEDEKTETTEVDHKHDDAETTLLKAILLQVICLYQMVVPHVVIQCTFDFSKLLKGIISEEGFQEEVPPVLQYYILKVALELPASKFAWLKGQESPNSGERSVFYLLMKMFVTSKHSQLKASTKLLITKILRDTGVFEYTWKELELWVEQLDNTAENRKEVVIQFLECVLLQLVTNPYSYTDKAADFVQEASTLQATVVKQDADNVSIPVSHIDDILDMVDVLMEDTEGLDEEVGFSLNEDMILLTFPFSAIVPTALESRNKLLLGNGNDSGESIVEYLIAVLTDLLHTQRDPLALCLMLQSYDSLQLSNTSLYTRIYQFNRYYNLWIPEQARETLEFQATRPTETSLVVGSAFSTLLTDTYERGVTLLLEENIQGKLREKIAQLPMQKVLLSVKQVMLYLRTTVENFAKLGKSSGPALLQLFMNLLSHLLNHCYHLDTCNQKKCEAAQAESAIFLDEESLATLELANDKTLEDALIVIFRHPTLESWYLALELQTLPSHNLNPILVKLLATHFNAGVLSLLKLSAPILQEMQRMDILSKYLEAITKSVLKELHTLKKTDPPIAYGKPSAQLEALYQLHPYMDGTQLRDITLALLALPKSTLLDQESKKSHQKKKHLSFLGETLKQLITESYQEQSQQGELLSSLEYVKRMSVLLPALVIEELETVFFQALQKEPILVHVVELDLLTYCLNRRTKTSLSIVALLIQQSSTHLLHFELWCLQGLGSCLPENFSLFLPLIHTYLQSCMQGDFTRPSKVSRNVMNVLRKVLWKELQNSLLGDDPQDLGIQEEVLSKLIPYSRAKELRLLIDKMPSILQKPEKSKSWIVSDAIALVLEKSVEELDVWKKTLLGSCIKWLIASYSSSIKEENTQDAEKSMLLRFNELLNSVNEVDPVDWQKFVKTGLKFRYHDYTFLKTLHACILLLYQPENSLCKNLVQLPLIYVMITQHSMFLPTILQTKEDDTNIQVKEMLVDLISTIVKLCPSVCESNHFAVLLGGYGATLSVLDQKILLLLRLYERNDLSLINFRMLLWGPAAVEHHKTCKSLGKSLWQQPSVGEILCLLDRERMMKTILHFPQNRKLLLKEDAQDIFKDKSKVDFDELYDPCFLLHLFSELTRPEFIVDCHKFVDSNALGLTVSALSSYDPQMRAAAYYVLASYYSHLEGARFREQPQLLYLLDILQNGIRKQNLRLTYTLTLFIAKTALQILKPEEHMYMKINKFLLSHQDLYLNKVPGFYNFFYSSDSEYRIEQEWIFELLRQGIRDTHCYDLYSSQRIFHIILSFFNSPLCDETMQNWIIEILQNAAQITKAAFEIVQEHSLLTWILHILGKKFLENQMLSNIICLLHTLWISNLGNKRMEIQEDHPCELVATQSPKLLPLQFVNEFLYVLVVLAKYLRPTLDSIQITHYFSTLSSVLRYRETVLEAFKEMDRLTVNENVFSTKDVLMLLHKWSIIMGDVKLQEDLKTVIEKYQVKELMKIIKDRNKPLAPARSKAQRNKKKMEVDPEETIDPELKASNLMKCRGLLRSILTHWDPAFAVVETEPKQERVSLAPDPEREAVDLVGATAVLVAQWEISSLMDPALDVQGIPEVLRWFKSSILLHPVVVSELVRDVTWRNSIFKLYSQLSHARELKAASQSVFCLFNTVMLHLMASQGPIENSFHEVVETIALSSTDEEDKTKQAAAVFLVSLYIKDIWLGAKPVNTFLTHIRMICDATELFSEDEEKAREQEEAIVALCENITVAT

Target ORF information:

RefSeq Version	XM_012544405.3
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii URB1 ribosome biogenesis homolog (URB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012544405.3

ORF Insert Sequence:

ATGGGAAACC CAAAGAGGAA AGCCCTTAAG GAAGAGAGCA AGGCCTCCTC GGCCGGTCCT 
GCGAAAAGGC CTCGCGGGGA AGATTTCACC GGGCAGCAGT TTAAGACACA GCTCAAAGAC 
CCTCAGACTA TGGTGTCAGC TTTGGAAACT TTTATCTCCA TTGCCAAAAT GTTACCACAA 
ACTGGACTAT ATGATGTCGT GGAAGGATAT ATAAAAATTT CTGTTGAGTG CTCAGAGATT 
TTCCAACTTT TAAATGGAGA AAAAAGAACT GAACATGAAA GGATATTAAT TTTTAAAACT 
TTAGAGGCCA TATTACTGCG AACAGCAGGT GATCTTTCTC ATTTTCATGT TGTGGGAACC 
AACATCATGA AAAAAGTTAT GACTAGCCAT ATGAAGCTGA TTTATGATTC ACTAAATGCT 
TCAAGCTACA AAATGAGTCA AACCTGTTTG AACCTTATGT CCGCTATGGT GGCACAGGGT 
CCAGATGCTG CCAGGGATTT CTATAGCCGA TTTGACTTCA ACAAAAATTC ATTGTATGGT 
TTGGTGAAAA TAAGAAGTCC AAAGGGGCTG CCAGATGTTC GATTGGCATA TATCCAGTTT 
GCCCTTTCCT TCTTAATTGC TGGTGATGAT AATACCATAG CACGAGTGTT GGAGTTGAAA 
GAATTCCTCC CTGTCATTTT TGCGACAGGC TTGAAGGAAG ACAGAATTTC TACTATCAAT 
CTTTTGTTGT CCACCCTGAA AACCAAGGTG GTTCACAATA TTGGTGTTAC AAAGACTCAG 
AAAGTTCGTT TCTTCACTGG ATATTTGTTA AATAATGTGG CTTCTCTCTA TCGTTGGAAT 
GGAATTGCTG ATGTCAGCGC AGAAAATATC AAGCCTACTG AAGAAGCAGG CAAGACCATG 
GTAAGGGAAC TGGTTCACAA CTTCTTAATG GATCTTTGCT GTTCTCTGAA GCATGGCATT 
AACTTCTATG ATGCATCTTT TGGTACTCTG GGCAGAGGAG GGAACTTGAC TCTCTTGAAC 
TTCTTACTGG GTTTGAAGAC AGCAGCAGAT GATGACTTGG TGGCTGAACT TGTAGTGAAC 
ATCCTAAAAG TATGCCCAGA CTTACTTACG AAGTACTTTA AGGAGGTGAC CTATTCCTTT 
GTTCCAAGGA TGAAGTCGGC TTGGTTAAAC AATATCAGGC TACTAAGGAA GATTTATGAG 
GCTCAGCCAG AGATTTCCCC AGCATTTAAA ACTAAAGAGT TTATACCCTT GTCTAGATTG 
CTTTCCATGG TCATGGTGAC AACAGTACCC AACGTGTGCA ACAAAATCAT GTTTACTCAA 
GGATTAAAAT TGGAAAACAA ATCAGTAAAT CATACAATTT TATCACTTAT TTCCACCATT 
TTGAAGAGAG CATTAAAAAA CATTGATCAC TGTCTCAATA AGGATATATG GCAGGAGTCA 
AGCATCTATA CTCCTGTGAT GATGGAGGAA TTTGTGCATC TGTACAGAGA AGCTGTAAGC 
AAGCTTTTGC CAGATTTGAA CAATATTGTA TGTGTGTGGC AATCACTTGG AAAGCAAAAA 
ATAGATGAAG ATGGCGGGTT AGAAAAAAAA AATTCCCTTG AAGATGAGAA AACTGAAACT 
ACAGAAGTAG ACCACAAACA TGATGATGCA GAAACAACTC TTTTGAAGGC TATTTTACTT 
CAGGTCATTT GTCTTTATCA GATGGTTGTT CCCCATGTTG TCATTCAATG TACATTTGAC 
TTCAGCAAGC TTCTAAAAGG TATCATTTCA GAAGAGGGAT TTCAAGAGGA AGTTCCTCCT 
GTCCTTCAGT ACTATATTCT GAAAGTGGCC CTAGAATTGC CTGCCAGCAA ATTTGCCTGG 
TTAAAAGGAC AGGAAAGTCC AAATAGTGGA GAAAGATCTG TGTTTTATTT GCTGATGAAA 
ATGTTTGTGA CAAGTAAACA CTCACAACTT AAAGCATCAA CAAAACTATT GATTACAAAG 
ATTTTACGTG ACACTGGAGT ATTTGAATAT ACATGGAAAG AGCTAGAACT ATGGGTGGAG 
CAATTAGATA ACACAGCAGA AAACAGAAAA GAAGTTGTGA TTCAGTTTTT GGAATGTGTT 
TTGTTGCAAT TGGTAACAAA TCCCTATTCA TATACAGACA AGGCAGCAGA TTTTGTTCAA 
GAAGCTAGTA CACTACAGGC CACTGTGGTG AAGCAAGATG CTGATAATGT TAGCATACCT 
GTATCACACA TTGATGATAT CCTGGATATG GTGGATGTCC TCATGGAGGA CACTGAAGGC 
TTAGATGAGG AAGTTGGTTT CTCTTTAAAT GAAGATATGA TTCTGCTGAC ATTCCCATTC 
AGCGCCATAG TTCCTACAGC TCTGGAGAGC AGGAATAAAT TGCTTCTTGG GAATGGAAAT 
GATTCAGGTG AAAGCATAGT GGAATATCTG ATAGCCGTTC TGACAGACCT TCTACATACA 
CAGCGAGATC CTTTGGCTCT TTGTCTGATG CTTCAGTCTT ATGACAGTTT ACAACTTTCA 
AACACATCTT TGTATACTCG GATTTACCAG TTCAACAGAT ACTATAACCT GTGGATACCT 
GAGCAAGCCA GAGAAACTTT GGAATTTCAA GCTACCAGAC CCACTGAGAC ATCACTTGTG 
GTTGGCTCTG CCTTTTCCAC CTTGTTAACA GATACCTATG AGAGGGGAGT TACCCTCCTT 
TTAGAAGAGA ATATCCAGGG AAAACTGAGA GAAAAAATTG CTCAGCTCCC AATGCAGAAG 
GTTCTGCTCT CTGTGAAGCA GGTGATGCTG TATCTTAGAA CCACTGTGGA GAACTTTGCC 
AAGCTTGGTA AAAGTTCAGG TCCTGCTTTG CTTCAGCTCT TCATGAATTT GCTCAGTCAC 
CTGTTGAACC ATTGTTATCA TTTGGACACA TGTAACCAGA AAAAATGTGA AGCTGCTCAG 
GCCGAATCTG CTATCTTTTT GGATGAGGAG TCCTTGGCCA CACTGGAATT AGCCAATGAT 
AAGACTCTGG AAGATGCGTT GATAGTAATC TTTAGACATC CCACATTGGA GAGCTGGTAT 
TTGGCCCTGG AGCTGCAGAC TCTTCCCTCA CATAACCTCA ATCCAATCCT TGTAAAGCTT 
TTAGCAACTC ATTTCAATGC TGGGGTCCTC TCACTCCTGA AACTGAGTGC CCCAATTCTG 
CAGGAAATGC AACGTATGGA CATCCTTTCC AAGTATTTAG AAGCCATTAC TAAGAGTGTC 
TTGAAAGAAC TACACACCCT GAAGAAGACA GATCCTCCCA TTGCATATGG GAAACCTTCA 
GCTCAGCTGG AAGCTTTGTA CCAACTACAT CCCTACATGG ATGGAACCCA GCTTAGGGAC 
ATTACGCTTG CACTCCTGGC TCTTCCTAAA TCAACCCTTT TGGATCAGGA ATCTAAGAAA 
TCTCACCAAA AAAAAAAACA TCTGAGCTTT TTAGGGGAAA CCCTCAAACA GCTTATCACT 
GAATCCTACC AGGAACAATC CCAGCAAGGT GAATTACTGT CGTCCCTAGA ATATGTGAAG 
AGAATGAGTG TCCTGTTGCC TGCACTGGTC ATTGAAGAGT TAGAGACTGT GTTCTTCCAA 
GCTTTACAGA AAGAGCCAAT TCTGGTCCAT GTAGTTGAGT TAGATTTATT GACCTACTGT 
CTAAATCGGA GGACAAAAAC TTCTCTTTCC ATTGTAGCTT TGTTGATTCA GCAAAGTAGT 
ACTCACCTGC TACACTTTGA GTTGTGGTGC TTACAGGGCC TAGGGTCCTG CCTTCCAGAA 
AACTTCAGCC TCTTTCTTCC ACTTATCCAT ACCTATTTAC AGTCTTGTAT GCAGGGAGAC 
TTTACACGTC CTTCTAAAGT GTCTAGAAAT GTCATGAATG TGTTGAGAAA GGTCCTGTGG 
AAAGAGCTTC AAAACAGTCT TTTAGGAGAT GATCCTCAGG ATCTTGGAAT TCAAGAAGAG 
GTCCTTTCTA AATTGATTCC CTATTCAAGA GCAAAAGAAC TTAGACTCCT CATTGACAAA 
ATGCCCAGCA TTCTTCAAAA GCCAGAGAAA TCGAAGAGCT GGATTGTATC AGATGCCATA 
GCATTAGTAT TGGAAAAATC TGTGGAAGAG CTGGATGTTT GGAAAAAGAC TTTGTTGGGG 
TCATGTATAA AATGGCTAAT AGCTTCATAC AGCAGTAGTA TCAAAGAAGA AAATACCCAG 
GATGCAGAGA AGTCTATGTT GTTGCGATTT AATGAATTAT TGAACTCAGT TAATGAAGTT 
GATCCTGTTG ATTGGCAGAA GTTTGTGAAG ACAGGCCTTA AGTTCCGCTA CCATGATTAC 
ACATTTTTAA AGACTCTTCA TGCTTGTATA CTGCTGTTAT ATCAGCCTGA AAATTCCCTT 
TGCAAAAACT TAGTGCAGCT GCCACTAATA TATGTGATGA TCACACAACA CTCTATGTTT 
TTGCCAACCA TATTGCAAAC AAAAGAAGAT GACACAAATA TCCAAGTAAA AGAAATGTTA 
GTGGACCTAA TATCAACTAT TGTAAAACTT TGCCCTTCCG TCTGTGAAAG CAACCACTTT 
GCAGTGCTAC TAGGAGGCTA TGGTGCAACC CTCAGTGTTC TAGATCAGAA GATATTGCTG 
CTTCTTAGAT TGTATGAAAG AAATGATCTG AGCCTCATAA ACTTCAGAAT GCTGCTATGG 
GGCCCTGCTG CTGTGGAACA TCACAAGACG TGTAAGAGCC TGGGAAAGTC ACTATGGCAG 
CAGCCAAGTG TGGGAGAAAT CTTATGTCTC CTGGATCGAG AGAGAATGAT GAAGACCATT 
CTTCACTTCC CACAGAATCG AAAACTTCTG CTAAAGGAGG ATGCACAGGA TATATTCAAA 
GACAAAAGCA AAGTAGACTT CGACGAACTT TATGATCCCT GTTTTCTACT TCACCTTTTC 
AGTGAACTTA CCAGACCAGA ATTTATAGTG GATTGTCACA AATTTGTTGA TTCCAATGCC 
CTGGGCCTCA CAGTTTCTGC CCTTAGCAGT TACGATCCTC AGATGCGAGC AGCAGCTTAT 
TATGTTCTGG CTTCCTATTA TTCCCATTTG GAAGGGGCTC GATTTCGAGA ACAGCCACAG 
CTGCTCTATC TCTTGGACAT ATTACAAAAT GGAATCAGAA AACAGAATCT CAGATTGACC 
TATACCTTGA CACTGTTTAT TGCCAAAACT GCCTTGCAGA TTCTGAAACC AGAGGAGCAC 
ATGTACATGA AGATAAACAA ATTCCTTTTG TCACATCAGG ACTTATACTT GAACAAAGTA 
CCGGGATTCT ATAACTTTTT CTACAGCTCT GATTCAGAGT ATAGGATAGA ACAGGAGTGG 
ATATTTGAAC TTTTGAGACA AGGAATTCGA GACACACACT GCTATGATTT GTATTCCTCT 
CAGAGAATTT TCCATATCAT CCTGTCCTTC TTCAACAGCC CATTATGTGA TGAAACAATG 
CAGAACTGGA TTATAGAAAT CTTACAAAAT GCTGCCCAGA TTACCAAAGC TGCCTTTGAA 
ATTGTGCAGG AACATAGCCT TTTAACTTGG ATTTTGCATA TCCTTGGGAA AAAGTTTTTG 
GAAAACCAGA TGCTATCCAA CATTATATGC TTGCTGCATA CTCTCTGGAT ATCCAACTTG 
GGAAACAAAA GAATGGAAAT ACAGGAAGAC CATCCTTGCG AGCTTGTGGC TACACAGTCA 
CCAAAGCTGC TTCCTCTACA ATTTGTCAAT GAATTCCTCT ATGTTTTGGT TGTGTTGGCG 
AAATATCTTC GGCCCACCTT GGACTCTATC CAGATCACTC ACTATTTTAG TACTTTGAGC 
TCTGTGCTGA GGTACCGAGA AACTGTTTTA GAAGCCTTCA AGGAAATGGA CCGATTGACT 
GTAAATGAAA ATGTATTTTC TACCAAAGAT GTCCTCATGC TCTTACATAA GTGGAGCATA 
ATTATGGGAG ATGTTAAACT CCAAGAAGAC TTGAAAACAG TGATTGAAAA ATACCAAGTC 
AAGGAATTAA TGAAAATTAT TAAAGATAGG AACAAACCCC TTGCACCAGC TCGATCTAAA 
GCTCAGCGTA ACAAAAAAAA GATGGAGGTG GATCCAGAGG AAACAATTGA TCCAGAGTTA 
AAGGCTTCCA ACCTGATGAA ATGCCGAGGT CTCCTGAGAT CCATACTGAC CCACTGGGAC 
CCCGCCTTTG CTGTGGTAGA GACAGAGCCA AAGCAAGAAC GAGTCTCCTT AGCTCCTGAC 
CCAGAGAGAG AAGCTGTGGA TCTTGTTGGG GCCACCGCTG TTCTGGTGGC CCAATGGGAG 
ATCAGCTCCC TCATGGACCC AGCGCTGGAT GTCCAGGGAA TTCCAGAGGT CCTCAGGTGG 
TTTAAAAGCA GCATTTTGCT TCATCCTGTG GTTGTCTCAG AACTTGTCAG AGATGTTACA 
TGGAGAAATA GCATCTTTAA GCTGTACAGC CAGCTTTCCC ATGCCAGAGA ACTGAAAGCA 
GCCTCTCAGA GCGTCTTCTG CCTCTTCAAT ACAGTCATGC TTCACTTAAT GGCATCCCAG 
GGCCCAATAG AGAACTCTTT TCATGAAGTG GTAGAAACAA TCGCTCTTTC TTCTACAGAT 
GAAGAAGACA AAACAAAACA AGCTGCTGCA GTGTTCCTGG TATCTCTTTA TATTAAAGAT 
ATCTGGCTAG GGGCAAAGCC AGTGAATACC TTCCTGACTC ACATCCGAAT GATCTGTGAT 
GCCACAGAGT TGTTCAGTGA GGATGAAGAG AAGGCTCGGG AACAAGAAGA AGCAATAGTA 
GCACTGTGTG AGAATATCAC TGTTGCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

URB1 ( XM_012544405.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012399859.1 Sarcophilus harrisii URB1 ribosome biogenesis 1 homolog (S. cerevisiae) (URB1), transcript variant X1, mRNA.

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CloneID	OSa125228
Clone ID Related Accession (Same CDS sequence)	XM_012544405.3 , XM_012544405.2 , XM_012544405.1
Accession Version	XM_012544405.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	nucleolar pre-ribosomal-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831550.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012544405.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACC CAAAGAGGAA AGCCCTTAAG GAAGAGAGCA AGGCCTCCTC GGCCGGTCCT 
GCGAAAAGGC CTCGCGGGGA AGATTTCACC GGGCAGCAGT TTAAGACACA GCTCAAAGAC 
CCTCAGACTA TGGTGTCAGC TTTGGAAACT TTTATCTCCA TTGCCAAAAT GTTACCACAA 
ACTGGACTAT ATGATGTCGT GGAAGGATAT ATAAAAATTT CTGTTGAGTG CTCAGAGATT 
TTCCAACTTT TAAATGGAGA AAAAAGAACT GAACATGAAA GGATATTAAT TTTTAAAACT 
TTAGAGGCCA TATTACTGCG AACAGCAGGT GATCTTTCTC ATTTTCATGT TGTGGGAACC 
AACATCATGA AAAAAGTTAT GACTAGCCAT ATGAAGCTGA TTTATGATTC ACTAAATGCT 
TCAAGCTACA AAATGAGTCA AACCTGTTTG AACCTTATGT CCGCTATGGT GGCACAGGGT 
CCAGATGCTG CCAGGGATTT CTATAGCCGA TTTGACTTCA ACAAAAATTC ATTGTATGGT 
TTGGTGAAAA TAAGAAGTCC AAAGGGGCTG CCAGATGTTC GATTGGCATA TATCCAGTTT 
GCCCTTTCCT TCTTAATTGC TGGTGATGAT AATACCATAG CACGAGTGTT GGAGTTGAAA 
GAATTCCTCC CTGTCATTTT TGCGACAGGC TTGAAGGAAG ACAGAATTTC TACTATCAAT 
CTTTTGTTGT CCACCCTGAA AACCAAGGTG GTTCACAATA TTGGTGTTAC AAAGACTCAG 
AAAGTTCGTT TCTTCACTGG ATATTTGTTA AATAATGTGG CTTCTCTCTA TCGTTGGAAT 
GGAATTGCTG ATGTCAGCGC AGAAAATATC AAGCCTACTG AAGAAGCAGG CAAGACCATG 
GTAAGGGAAC TGGTTCACAA CTTCTTAATG GATCTTTGCT GTTCTCTGAA GCATGGCATT 
AACTTCTATG ATGCATCTTT TGGTACTCTG GGCAGAGGAG GGAACTTGAC TCTCTTGAAC 
TTCTTACTGG GTTTGAAGAC AGCAGCAGAT GATGACTTGG TGGCTGAACT TGTAGTGAAC 
ATCCTAAAAG TATGCCCAGA CTTACTTACG AAGTACTTTA AGGAGGTGAC CTATTCCTTT 
GTTCCAAGGA TGAAGTCGGC TTGGTTAAAC AATATCAGGC TACTAAGGAA GATTTATGAG 
GCTCAGCCAG AGATTTCCCC AGCATTTAAA ACTAAAGAGT TTATACCCTT GTCTAGATTG 
CTTTCCATGG TCATGGTGAC AACAGTACCC AACGTGTGCA ACAAAATCAT GTTTACTCAA 
GGATTAAAAT TGGAAAACAA ATCAGTAAAT CATACAATTT TATCACTTAT TTCCACCATT 
TTGAAGAGAG CATTAAAAAA CATTGATCAC TGTCTCAATA AGGATATATG GCAGGAGTCA 
AGCATCTATA CTCCTGTGAT GATGGAGGAA TTTGTGCATC TGTACAGAGA AGCTGTAAGC 
AAGCTTTTGC CAGATTTGAA CAATATTGTA TGTGTGTGGC AATCACTTGG AAAGCAAAAA 
ATAGATGAAG ATGGCGGGTT AGAAAAAAAA AATTCCCTTG AAGATGAGAA AACTGAAACT 
ACAGAAGTAG ACCACAAACA TGATGATGCA GAAACAACTC TTTTGAAGGC TATTTTACTT 
CAGGTCATTT GTCTTTATCA GATGGTTGTT CCCCATGTTG TCATTCAATG TACATTTGAC 
TTCAGCAAGC TTCTAAAAGG TATCATTTCA GAAGAGGGAT TTCAAGAGGA AGTTCCTCCT 
GTCCTTCAGT ACTATATTCT GAAAGTGGCC CTAGAATTGC CTGCCAGCAA ATTTGCCTGG 
TTAAAAGGAC AGGAAAGTCC AAATAGTGGA GAAAGATCTG TGTTTTATTT GCTGATGAAA 
ATGTTTGTGA CAAGTAAACA CTCACAACTT AAAGCATCAA CAAAACTATT GATTACAAAG 
ATTTTACGTG ACACTGGAGT ATTTGAATAT ACATGGAAAG AGCTAGAACT ATGGGTGGAG 
CAATTAGATA ACACAGCAGA AAACAGAAAA GAAGTTGTGA TTCAGTTTTT GGAATGTGTT 
TTGTTGCAAT TGGTAACAAA TCCCTATTCA TATACAGACA AGGCAGCAGA TTTTGTTCAA 
GAAGCTAGTA CACTACAGGC CACTGTGGTG AAGCAAGATG CTGATAATGT TAGCATACCT 
GTATCACACA TTGATGATAT CCTGGATATG GTGGATGTCC TCATGGAGGA CACTGAAGGC 
TTAGATGAGG AAGTTGGTTT CTCTTTAAAT GAAGATATGA TTCTGCTGAC ATTCCCATTC 
AGCGCCATAG TTCCTACAGC TCTGGAGAGC AGGAATAAAT TGCTTCTTGG GAATGGAAAT 
GATTCAGGTG AAAGCATAGT GGAATATCTG ATAGCCGTTC TGACAGACCT TCTACATACA 
CAGCGAGATC CTTTGGCTCT TTGTCTGATG CTTCAGTCTT ATGACAGTTT ACAACTTTCA 
AACACATCTT TGTATACTCG GATTTACCAG TTCAACAGAT ACTATAACCT GTGGATACCT 
GAGCAAGCCA GAGAAACTTT GGAATTTCAA GCTACCAGAC CCACTGAGAC ATCACTTGTG 
GTTGGCTCTG CCTTTTCCAC CTTGTTAACA GATACCTATG AGAGGGGAGT TACCCTCCTT 
TTAGAAGAGA ATATCCAGGG AAAACTGAGA GAAAAAATTG CTCAGCTCCC AATGCAGAAG 
GTTCTGCTCT CTGTGAAGCA GGTGATGCTG TATCTTAGAA CCACTGTGGA GAACTTTGCC 
AAGCTTGGTA AAAGTTCAGG TCCTGCTTTG CTTCAGCTCT TCATGAATTT GCTCAGTCAC 
CTGTTGAACC ATTGTTATCA TTTGGACACA TGTAACCAGA AAAAATGTGA AGCTGCTCAG 
GCCGAATCTG CTATCTTTTT GGATGAGGAG TCCTTGGCCA CACTGGAATT AGCCAATGAT 
AAGACTCTGG AAGATGCGTT GATAGTAATC TTTAGACATC CCACATTGGA GAGCTGGTAT 
TTGGCCCTGG AGCTGCAGAC TCTTCCCTCA CATAACCTCA ATCCAATCCT TGTAAAGCTT 
TTAGCAACTC ATTTCAATGC TGGGGTCCTC TCACTCCTGA AACTGAGTGC CCCAATTCTG 
CAGGAAATGC AACGTATGGA CATCCTTTCC AAGTATTTAG AAGCCATTAC TAAGAGTGTC 
TTGAAAGAAC TACACACCCT GAAGAAGACA GATCCTCCCA TTGCATATGG GAAACCTTCA 
GCTCAGCTGG AAGCTTTGTA CCAACTACAT CCCTACATGG ATGGAACCCA GCTTAGGGAC 
ATTACGCTTG CACTCCTGGC TCTTCCTAAA TCAACCCTTT TGGATCAGGA ATCTAAGAAA 
TCTCACCAAA AAAAAAAACA TCTGAGCTTT TTAGGGGAAA CCCTCAAACA GCTTATCACT 
GAATCCTACC AGGAACAATC CCAGCAAGGT GAATTACTGT CGTCCCTAGA ATATGTGAAG 
AGAATGAGTG TCCTGTTGCC TGCACTGGTC ATTGAAGAGT TAGAGACTGT GTTCTTCCAA 
GCTTTACAGA AAGAGCCAAT TCTGGTCCAT GTAGTTGAGT TAGATTTATT GACCTACTGT 
CTAAATCGGA GGACAAAAAC TTCTCTTTCC ATTGTAGCTT TGTTGATTCA GCAAAGTAGT 
ACTCACCTGC TACACTTTGA GTTGTGGTGC TTACAGGGCC TAGGGTCCTG CCTTCCAGAA 
AACTTCAGCC TCTTTCTTCC ACTTATCCAT ACCTATTTAC AGTCTTGTAT GCAGGGAGAC 
TTTACACGTC CTTCTAAAGT GTCTAGAAAT GTCATGAATG TGTTGAGAAA GGTCCTGTGG 
AAAGAGCTTC AAAACAGTCT TTTAGGAGAT GATCCTCAGG ATCTTGGAAT TCAAGAAGAG 
GTCCTTTCTA AATTGATTCC CTATTCAAGA GCAAAAGAAC TTAGACTCCT CATTGACAAA 
ATGCCCAGCA TTCTTCAAAA GCCAGAGAAA TCGAAGAGCT GGATTGTATC AGATGCCATA 
GCATTAGTAT TGGAAAAATC TGTGGAAGAG CTGGATGTTT GGAAAAAGAC TTTGTTGGGG 
TCATGTATAA AATGGCTAAT AGCTTCATAC AGCAGTAGTA TCAAAGAAGA AAATACCCAG 
GATGCAGAGA AGTCTATGTT GTTGCGATTT AATGAATTAT TGAACTCAGT TAATGAAGTT 
GATCCTGTTG ATTGGCAGAA GTTTGTGAAG ACAGGCCTTA AGTTCCGCTA CCATGATTAC 
ACATTTTTAA AGACTCTTCA TGCTTGTATA CTGCTGTTAT ATCAGCCTGA AAATTCCCTT 
TGCAAAAACT TAGTGCAGCT GCCACTAATA TATGTGATGA TCACACAACA CTCTATGTTT 
TTGCCAACCA TATTGCAAAC AAAAGAAGAT GACACAAATA TCCAAGTAAA AGAAATGTTA 
GTGGACCTAA TATCAACTAT TGTAAAACTT TGCCCTTCCG TCTGTGAAAG CAACCACTTT 
GCAGTGCTAC TAGGAGGCTA TGGTGCAACC CTCAGTGTTC TAGATCAGAA GATATTGCTG 
CTTCTTAGAT TGTATGAAAG AAATGATCTG AGCCTCATAA ACTTCAGAAT GCTGCTATGG 
GGCCCTGCTG CTGTGGAACA TCACAAGACG TGTAAGAGCC TGGGAAAGTC ACTATGGCAG 
CAGCCAAGTG TGGGAGAAAT CTTATGTCTC CTGGATCGAG AGAGAATGAT GAAGACCATT 
CTTCACTTCC CACAGAATCG AAAACTTCTG CTAAAGGAGG ATGCACAGGA TATATTCAAA 
GACAAAAGCA AAGTAGACTT CGACGAACTT TATGATCCCT GTTTTCTACT TCACCTTTTC 
AGTGAACTTA CCAGACCAGA ATTTATAGTG GATTGTCACA AATTTGTTGA TTCCAATGCC 
CTGGGCCTCA CAGTTTCTGC CCTTAGCAGT TACGATCCTC AGATGCGAGC AGCAGCTTAT 
TATGTTCTGG CTTCCTATTA TTCCCATTTG GAAGGGGCTC GATTTCGAGA ACAGCCACAG 
CTGCTCTATC TCTTGGACAT ATTACAAAAT GGAATCAGAA AACAGAATCT CAGATTGACC 
TATACCTTGA CACTGTTTAT TGCCAAAACT GCCTTGCAGA TTCTGAAACC AGAGGAGCAC 
ATGTACATGA AGATAAACAA ATTCCTTTTG TCACATCAGG ACTTATACTT GAACAAAGTA 
CCGGGATTCT ATAACTTTTT CTACAGCTCT GATTCAGAGT ATAGGATAGA ACAGGAGTGG 
ATATTTGAAC TTTTGAGACA AGGAATTCGA GACACACACT GCTATGATTT GTATTCCTCT 
CAGAGAATTT TCCATATCAT CCTGTCCTTC TTCAACAGCC CATTATGTGA TGAAACAATG 
CAGAACTGGA TTATAGAAAT CTTACAAAAT GCTGCCCAGA TTACCAAAGC TGCCTTTGAA 
ATTGTGCAGG AACATAGCCT TTTAACTTGG ATTTTGCATA TCCTTGGGAA AAAGTTTTTG 
GAAAACCAGA TGCTATCCAA CATTATATGC TTGCTGCATA CTCTCTGGAT ATCCAACTTG 
GGAAACAAAA GAATGGAAAT ACAGGAAGAC CATCCTTGCG AGCTTGTGGC TACACAGTCA 
CCAAAGCTGC TTCCTCTACA ATTTGTCAAT GAATTCCTCT ATGTTTTGGT TGTGTTGGCG 
AAATATCTTC GGCCCACCTT GGACTCTATC CAGATCACTC ACTATTTTAG TACTTTGAGC 
TCTGTGCTGA GGTACCGAGA AACTGTTTTA GAAGCCTTCA AGGAAATGGA CCGATTGACT 
GTAAATGAAA ATGTATTTTC TACCAAAGAT GTCCTCATGC TCTTACATAA GTGGAGCATA 
ATTATGGGAG ATGTTAAACT CCAAGAAGAC TTGAAAACAG TGATTGAAAA ATACCAAGTC 
AAGGAATTAA TGAAAATTAT TAAAGATAGG AACAAACCCC TTGCACCAGC TCGATCTAAA 
GCTCAGCGTA ACAAAAAAAA GATGGAGGTG GATCCAGAGG AAACAATTGA TCCAGAGTTA 
AAGGCTTCCA ACCTGATGAA ATGCCGAGGT CTCCTGAGAT CCATACTGAC CCACTGGGAC 
CCCGCCTTTG CTGTGGTAGA GACAGAGCCA AAGCAAGAAC GAGTCTCCTT AGCTCCTGAC 
CCAGAGAGAG AAGCTGTGGA TCTTGTTGGG GCCACCGCTG TTCTGGTGGC CCAATGGGAG 
ATCAGCTCCC TCATGGACCC AGCGCTGGAT GTCCAGGGAA TTCCAGAGGT CCTCAGGTGG 
TTTAAAAGCA GCATTTTGCT TCATCCTGTG GTTGTCTCAG AACTTGTCAG AGATGTTACA 
TGGAGAAATA GCATCTTTAA GCTGTACAGC CAGCTTTCCC ATGCCAGAGA ACTGAAAGCA 
GCCTCTCAGA GCGTCTTCTG CCTCTTCAAT ACAGTCATGC TTCACTTAAT GGCATCCCAG 
GGCCCAATAG AGAACTCTTT TCATGAAGTG GTAGAAACAA TCGCTCTTTC TTCTACAGAT 
GAAGAAGACA AAACAAAACA AGCTGCTGCA GTGTTCCTGG TATCTCTTTA TATTAAAGAT 
ATCTGGCTAG GGGCAAAGCC AGTGAATACC TTCCTGACTC ACATCCGAAT GATCTGTGAT 
GCCACAGAGT TGTTCAGTGA GGATGAAGAG AAGGCTCGGG AACAAGAAGA AGCAATAGTA 
GCACTGTGTG AGAATATCAC TGTTGCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012399859.1
CDS	111..6923
Translation	MGNPKRKALKEESKASSAGPAKRPRGEDFTGQQFKTQLKDPQTMVSALETFISIAKMLPQTGLYDVVEGYIKISVECSEIFQLLNGEKRTEHERILIFKTLEAILLRTAGDLSHFHVVGTNIMKKVMTSHMKLIYDSLNASSYKMSQTCLNLMSAMVAQGPDAARDFYSRFDFNKNSLYGLVKIRSPKGLPDVRLAYIQFALSFLIAGDDNTIARVLELKEFLPVIFATGLKEDRISTINLLLSTLKTKVVHNIGVTKTQKVRFFTGYLLNNVASLYRWNGIADVSAENIKPTEEAGKTMVRELVHNFLMDLCCSLKHGINFYDASFGTLGRGGNLTLLNFLLGLKTAADDDLVAELVVNILKVCPDLLTKYFKEVTYSFVPRMKSAWLNNIRLLRKIYEAQPEISPAFKTKEFIPLSRLLSMVMVTTVPNVCNKIMFTQGLKLENKSVNHTILSLISTILKRALKNIDHCLNKDIWQESSIYTPVMMEEFVHLYREAVSKLLPDLNNIVCVWQSLGKQKIDEDGGLEKKNSLEDEKTETTEVDHKHDDAETTLLKAILLQVICLYQMVVPHVVIQCTFDFSKLLKGIISEEGFQEEVPPVLQYYILKVALELPASKFAWLKGQESPNSGERSVFYLLMKMFVTSKHSQLKASTKLLITKILRDTGVFEYTWKELELWVEQLDNTAENRKEVVIQFLECVLLQLVTNPYSYTDKAADFVQEASTLQATVVKQDADNVSIPVSHIDDILDMVDVLMEDTEGLDEEVGFSLNEDMILLTFPFSAIVPTALESRNKLLLGNGNDSGESIVEYLIAVLTDLLHTQRDPLALCLMLQSYDSLQLSNTSLYTRIYQFNRYYNLWIPEQARETLEFQATRPTETSLVVGSAFSTLLTDTYERGVTLLLEENIQGKLREKIAQLPMQKVLLSVKQVMLYLRTTVENFAKLGKSSGPALLQLFMNLLSHLLNHCYHLDTCNQKKCEAAQAESAIFLDEESLATLELANDKTLEDALIVIFRHPTLESWYLALELQTLPSHNLNPILVKLLATHFNAGVLSLLKLSAPILQEMQRMDILSKYLEAITKSVLKELHTLKKTDPPIAYGKPSAQLEALYQLHPYMDGTQLRDITLALLALPKSTLLDQESKKSHQKKKHLSFLGETLKQLITESYQEQSQQGELLSSLEYVKRMSVLLPALVIEELETVFFQALQKEPILVHVVELDLLTYCLNRRTKTSLSIVALLIQQSSTHLLHFELWCLQGLGSCLPENFSLFLPLIHTYLQSCMQGDFTRPSKVSRNVMNVLRKVLWKELQNSLLGDDPQDLGIQEEVLSKLIPYSRAKELRLLIDKMPSILQKPEKSKSWIVSDAIALVLEKSVEELDVWKKTLLGSCIKWLIASYSSSIKEENTQDAEKSMLLRFNELLNSVNEVDPVDWQKFVKTGLKFRYHDYTFLKTLHACILLLYQPENSLCKNLVQLPLIYVMITQHSMFLPTILQTKEDDTNIQVKEMLVDLISTIVKLCPSVCESNHFAVLLGGYGATLSVLDQKILLLLRLYERNDLSLINFRMLLWGPAAVEHHKTCKSLGKSLWQQPSVGEILCLLDRERMMKTILHFPQNRKLLLKEDAQDIFKDKSKVDFDELYDPCFLLHLFSELTRPEFIVDCHKFVDSNALGLTVSALSSYDPQMRAAAYYVLASYYSHLEGARFREQPQLLYLLDILQNGIRKQNLRLTYTLTLFIAKTALQILKPEEHMYMKINKFLLSHQDLYLNKVPGFYNFFYSSDSEYRIEQEWIFELLRQGIRDTHCYDLYSSQRIFHIILSFFNSPLCDETMQNWIIEILQNAAQITKAAFEIVQEHSLLTWILHILGKKFLENQMLSNIICLLHTLWISNLGNKRMEIQEDHPCELVATQSPKLLPLQFVNEFLYVLVVLAKYLRPTLDSIQITHYFSTLSSVLRYRETVLEAFKEMDRLTVNENVFSTKDVLMLLHKWSIIMGDVKLQEDLKTVIEKYQVKELMKIIKDRNKPLAPARSKAQRNKKKMEVDPEETIDPELKASNLMKCRGLLRSILTHWDPAFAVVETEPKQERVSLAPDPEREAVDLVGATAVLVAQWEISSLMDPALDVQGIPEVLRWFKSSILLHPVVVSELVRDVTWRNSIFKLYSQLSHARELKAASQSVFCLFNTVMLHLMASQGPIENSFHEVVETIALSSTDEEDKTKQAAAVFLVSLYIKDIWLGAKPVNTFLTHIRMICDATELFSEDEEKAREQEEAIVALCENITVAT

Target ORF information:

RefSeq Version	XM_012544405.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii URB1 ribosome biogenesis 1 homolog (S. cerevisiae) (URB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012544405.2

ORF Insert Sequence:

ATGGGAAACC CAAAGAGGAA AGCCCTTAAG GAAGAGAGCA AGGCCTCCTC GGCCGGTCCT 
GCGAAAAGGC CTCGCGGGGA AGATTTCACC GGGCAGCAGT TTAAGACACA GCTCAAAGAC 
CCTCAGACTA TGGTGTCAGC TTTGGAAACT TTTATCTCCA TTGCCAAAAT GTTACCACAA 
ACTGGACTAT ATGATGTCGT GGAAGGATAT ATAAAAATTT CTGTTGAGTG CTCAGAGATT 
TTCCAACTTT TAAATGGAGA AAAAAGAACT GAACATGAAA GGATATTAAT TTTTAAAACT 
TTAGAGGCCA TATTACTGCG AACAGCAGGT GATCTTTCTC ATTTTCATGT TGTGGGAACC 
AACATCATGA AAAAAGTTAT GACTAGCCAT ATGAAGCTGA TTTATGATTC ACTAAATGCT 
TCAAGCTACA AAATGAGTCA AACCTGTTTG AACCTTATGT CCGCTATGGT GGCACAGGGT 
CCAGATGCTG CCAGGGATTT CTATAGCCGA TTTGACTTCA ACAAAAATTC ATTGTATGGT 
TTGGTGAAAA TAAGAAGTCC AAAGGGGCTG CCAGATGTTC GATTGGCATA TATCCAGTTT 
GCCCTTTCCT TCTTAATTGC TGGTGATGAT AATACCATAG CACGAGTGTT GGAGTTGAAA 
GAATTCCTCC CTGTCATTTT TGCGACAGGC TTGAAGGAAG ACAGAATTTC TACTATCAAT 
CTTTTGTTGT CCACCCTGAA AACCAAGGTG GTTCACAATA TTGGTGTTAC AAAGACTCAG 
AAAGTTCGTT TCTTCACTGG ATATTTGTTA AATAATGTGG CTTCTCTCTA TCGTTGGAAT 
GGAATTGCTG ATGTCAGCGC AGAAAATATC AAGCCTACTG AAGAAGCAGG CAAGACCATG 
GTAAGGGAAC TGGTTCACAA CTTCTTAATG GATCTTTGCT GTTCTCTGAA GCATGGCATT 
AACTTCTATG ATGCATCTTT TGGTACTCTG GGCAGAGGAG GGAACTTGAC TCTCTTGAAC 
TTCTTACTGG GTTTGAAGAC AGCAGCAGAT GATGACTTGG TGGCTGAACT TGTAGTGAAC 
ATCCTAAAAG TATGCCCAGA CTTACTTACG AAGTACTTTA AGGAGGTGAC CTATTCCTTT 
GTTCCAAGGA TGAAGTCGGC TTGGTTAAAC AATATCAGGC TACTAAGGAA GATTTATGAG 
GCTCAGCCAG AGATTTCCCC AGCATTTAAA ACTAAAGAGT TTATACCCTT GTCTAGATTG 
CTTTCCATGG TCATGGTGAC AACAGTACCC AACGTGTGCA ACAAAATCAT GTTTACTCAA 
GGATTAAAAT TGGAAAACAA ATCAGTAAAT CATACAATTT TATCACTTAT TTCCACCATT 
TTGAAGAGAG CATTAAAAAA CATTGATCAC TGTCTCAATA AGGATATATG GCAGGAGTCA 
AGCATCTATA CTCCTGTGAT GATGGAGGAA TTTGTGCATC TGTACAGAGA AGCTGTAAGC 
AAGCTTTTGC CAGATTTGAA CAATATTGTA TGTGTGTGGC AATCACTTGG AAAGCAAAAA 
ATAGATGAAG ATGGCGGGTT AGAAAAAAAA AATTCCCTTG AAGATGAGAA AACTGAAACT 
ACAGAAGTAG ACCACAAACA TGATGATGCA GAAACAACTC TTTTGAAGGC TATTTTACTT 
CAGGTCATTT GTCTTTATCA GATGGTTGTT CCCCATGTTG TCATTCAATG TACATTTGAC 
TTCAGCAAGC TTCTAAAAGG TATCATTTCA GAAGAGGGAT TTCAAGAGGA AGTTCCTCCT 
GTCCTTCAGT ACTATATTCT GAAAGTGGCC CTAGAATTGC CTGCCAGCAA ATTTGCCTGG 
TTAAAAGGAC AGGAAAGTCC AAATAGTGGA GAAAGATCTG TGTTTTATTT GCTGATGAAA 
ATGTTTGTGA CAAGTAAACA CTCACAACTT AAAGCATCAA CAAAACTATT GATTACAAAG 
ATTTTACGTG ACACTGGAGT ATTTGAATAT ACATGGAAAG AGCTAGAACT ATGGGTGGAG 
CAATTAGATA ACACAGCAGA AAACAGAAAA GAAGTTGTGA TTCAGTTTTT GGAATGTGTT 
TTGTTGCAAT TGGTAACAAA TCCCTATTCA TATACAGACA AGGCAGCAGA TTTTGTTCAA 
GAAGCTAGTA CACTACAGGC CACTGTGGTG AAGCAAGATG CTGATAATGT TAGCATACCT 
GTATCACACA TTGATGATAT CCTGGATATG GTGGATGTCC TCATGGAGGA CACTGAAGGC 
TTAGATGAGG AAGTTGGTTT CTCTTTAAAT GAAGATATGA TTCTGCTGAC ATTCCCATTC 
AGCGCCATAG TTCCTACAGC TCTGGAGAGC AGGAATAAAT TGCTTCTTGG GAATGGAAAT 
GATTCAGGTG AAAGCATAGT GGAATATCTG ATAGCCGTTC TGACAGACCT TCTACATACA 
CAGCGAGATC CTTTGGCTCT TTGTCTGATG CTTCAGTCTT ATGACAGTTT ACAACTTTCA 
AACACATCTT TGTATACTCG GATTTACCAG TTCAACAGAT ACTATAACCT GTGGATACCT 
GAGCAAGCCA GAGAAACTTT GGAATTTCAA GCTACCAGAC CCACTGAGAC ATCACTTGTG 
GTTGGCTCTG CCTTTTCCAC CTTGTTAACA GATACCTATG AGAGGGGAGT TACCCTCCTT 
TTAGAAGAGA ATATCCAGGG AAAACTGAGA GAAAAAATTG CTCAGCTCCC AATGCAGAAG 
GTTCTGCTCT CTGTGAAGCA GGTGATGCTG TATCTTAGAA CCACTGTGGA GAACTTTGCC 
AAGCTTGGTA AAAGTTCAGG TCCTGCTTTG CTTCAGCTCT TCATGAATTT GCTCAGTCAC 
CTGTTGAACC ATTGTTATCA TTTGGACACA TGTAACCAGA AAAAATGTGA AGCTGCTCAG 
GCCGAATCTG CTATCTTTTT GGATGAGGAG TCCTTGGCCA CACTGGAATT AGCCAATGAT 
AAGACTCTGG AAGATGCGTT GATAGTAATC TTTAGACATC CCACATTGGA GAGCTGGTAT 
TTGGCCCTGG AGCTGCAGAC TCTTCCCTCA CATAACCTCA ATCCAATCCT TGTAAAGCTT 
TTAGCAACTC ATTTCAATGC TGGGGTCCTC TCACTCCTGA AACTGAGTGC CCCAATTCTG 
CAGGAAATGC AACGTATGGA CATCCTTTCC AAGTATTTAG AAGCCATTAC TAAGAGTGTC 
TTGAAAGAAC TACACACCCT GAAGAAGACA GATCCTCCCA TTGCATATGG GAAACCTTCA 
GCTCAGCTGG AAGCTTTGTA CCAACTACAT CCCTACATGG ATGGAACCCA GCTTAGGGAC 
ATTACGCTTG CACTCCTGGC TCTTCCTAAA TCAACCCTTT TGGATCAGGA ATCTAAGAAA 
TCTCACCAAA AAAAAAAACA TCTGAGCTTT TTAGGGGAAA CCCTCAAACA GCTTATCACT 
GAATCCTACC AGGAACAATC CCAGCAAGGT GAATTACTGT CGTCCCTAGA ATATGTGAAG 
AGAATGAGTG TCCTGTTGCC TGCACTGGTC ATTGAAGAGT TAGAGACTGT GTTCTTCCAA 
GCTTTACAGA AAGAGCCAAT TCTGGTCCAT GTAGTTGAGT TAGATTTATT GACCTACTGT 
CTAAATCGGA GGACAAAAAC TTCTCTTTCC ATTGTAGCTT TGTTGATTCA GCAAAGTAGT 
ACTCACCTGC TACACTTTGA GTTGTGGTGC TTACAGGGCC TAGGGTCCTG CCTTCCAGAA 
AACTTCAGCC TCTTTCTTCC ACTTATCCAT ACCTATTTAC AGTCTTGTAT GCAGGGAGAC 
TTTACACGTC CTTCTAAAGT GTCTAGAAAT GTCATGAATG TGTTGAGAAA GGTCCTGTGG 
AAAGAGCTTC AAAACAGTCT TTTAGGAGAT GATCCTCAGG ATCTTGGAAT TCAAGAAGAG 
GTCCTTTCTA AATTGATTCC CTATTCAAGA GCAAAAGAAC TTAGACTCCT CATTGACAAA 
ATGCCCAGCA TTCTTCAAAA GCCAGAGAAA TCGAAGAGCT GGATTGTATC AGATGCCATA 
GCATTAGTAT TGGAAAAATC TGTGGAAGAG CTGGATGTTT GGAAAAAGAC TTTGTTGGGG 
TCATGTATAA AATGGCTAAT AGCTTCATAC AGCAGTAGTA TCAAAGAAGA AAATACCCAG 
GATGCAGAGA AGTCTATGTT GTTGCGATTT AATGAATTAT TGAACTCAGT TAATGAAGTT 
GATCCTGTTG ATTGGCAGAA GTTTGTGAAG ACAGGCCTTA AGTTCCGCTA CCATGATTAC 
ACATTTTTAA AGACTCTTCA TGCTTGTATA CTGCTGTTAT ATCAGCCTGA AAATTCCCTT 
TGCAAAAACT TAGTGCAGCT GCCACTAATA TATGTGATGA TCACACAACA CTCTATGTTT 
TTGCCAACCA TATTGCAAAC AAAAGAAGAT GACACAAATA TCCAAGTAAA AGAAATGTTA 
GTGGACCTAA TATCAACTAT TGTAAAACTT TGCCCTTCCG TCTGTGAAAG CAACCACTTT 
GCAGTGCTAC TAGGAGGCTA TGGTGCAACC CTCAGTGTTC TAGATCAGAA GATATTGCTG 
CTTCTTAGAT TGTATGAAAG AAATGATCTG AGCCTCATAA ACTTCAGAAT GCTGCTATGG 
GGCCCTGCTG CTGTGGAACA TCACAAGACG TGTAAGAGCC TGGGAAAGTC ACTATGGCAG 
CAGCCAAGTG TGGGAGAAAT CTTATGTCTC CTGGATCGAG AGAGAATGAT GAAGACCATT 
CTTCACTTCC CACAGAATCG AAAACTTCTG CTAAAGGAGG ATGCACAGGA TATATTCAAA 
GACAAAAGCA AAGTAGACTT CGACGAACTT TATGATCCCT GTTTTCTACT TCACCTTTTC 
AGTGAACTTA CCAGACCAGA ATTTATAGTG GATTGTCACA AATTTGTTGA TTCCAATGCC 
CTGGGCCTCA CAGTTTCTGC CCTTAGCAGT TACGATCCTC AGATGCGAGC AGCAGCTTAT 
TATGTTCTGG CTTCCTATTA TTCCCATTTG GAAGGGGCTC GATTTCGAGA ACAGCCACAG 
CTGCTCTATC TCTTGGACAT ATTACAAAAT GGAATCAGAA AACAGAATCT CAGATTGACC 
TATACCTTGA CACTGTTTAT TGCCAAAACT GCCTTGCAGA TTCTGAAACC AGAGGAGCAC 
ATGTACATGA AGATAAACAA ATTCCTTTTG TCACATCAGG ACTTATACTT GAACAAAGTA 
CCGGGATTCT ATAACTTTTT CTACAGCTCT GATTCAGAGT ATAGGATAGA ACAGGAGTGG 
ATATTTGAAC TTTTGAGACA AGGAATTCGA GACACACACT GCTATGATTT GTATTCCTCT 
CAGAGAATTT TCCATATCAT CCTGTCCTTC TTCAACAGCC CATTATGTGA TGAAACAATG 
CAGAACTGGA TTATAGAAAT CTTACAAAAT GCTGCCCAGA TTACCAAAGC TGCCTTTGAA 
ATTGTGCAGG AACATAGCCT TTTAACTTGG ATTTTGCATA TCCTTGGGAA AAAGTTTTTG 
GAAAACCAGA TGCTATCCAA CATTATATGC TTGCTGCATA CTCTCTGGAT ATCCAACTTG 
GGAAACAAAA GAATGGAAAT ACAGGAAGAC CATCCTTGCG AGCTTGTGGC TACACAGTCA 
CCAAAGCTGC TTCCTCTACA ATTTGTCAAT GAATTCCTCT ATGTTTTGGT TGTGTTGGCG 
AAATATCTTC GGCCCACCTT GGACTCTATC CAGATCACTC ACTATTTTAG TACTTTGAGC 
TCTGTGCTGA GGTACCGAGA AACTGTTTTA GAAGCCTTCA AGGAAATGGA CCGATTGACT 
GTAAATGAAA ATGTATTTTC TACCAAAGAT GTCCTCATGC TCTTACATAA GTGGAGCATA 
ATTATGGGAG ATGTTAAACT CCAAGAAGAC TTGAAAACAG TGATTGAAAA ATACCAAGTC 
AAGGAATTAA TGAAAATTAT TAAAGATAGG AACAAACCCC TTGCACCAGC TCGATCTAAA 
GCTCAGCGTA ACAAAAAAAA GATGGAGGTG GATCCAGAGG AAACAATTGA TCCAGAGTTA 
AAGGCTTCCA ACCTGATGAA ATGCCGAGGT CTCCTGAGAT CCATACTGAC CCACTGGGAC 
CCCGCCTTTG CTGTGGTAGA GACAGAGCCA AAGCAAGAAC GAGTCTCCTT AGCTCCTGAC 
CCAGAGAGAG AAGCTGTGGA TCTTGTTGGG GCCACCGCTG TTCTGGTGGC CCAATGGGAG 
ATCAGCTCCC TCATGGACCC AGCGCTGGAT GTCCAGGGAA TTCCAGAGGT CCTCAGGTGG 
TTTAAAAGCA GCATTTTGCT TCATCCTGTG GTTGTCTCAG AACTTGTCAG AGATGTTACA 
TGGAGAAATA GCATCTTTAA GCTGTACAGC CAGCTTTCCC ATGCCAGAGA ACTGAAAGCA 
GCCTCTCAGA GCGTCTTCTG CCTCTTCAAT ACAGTCATGC TTCACTTAAT GGCATCCCAG 
GGCCCAATAG AGAACTCTTT TCATGAAGTG GTAGAAACAA TCGCTCTTTC TTCTACAGAT 
GAAGAAGACA AAACAAAACA AGCTGCTGCA GTGTTCCTGG TATCTCTTTA TATTAAAGAT 
ATCTGGCTAG GGGCAAAGCC AGTGAATACC TTCCTGACTC ACATCCGAAT GATCTGTGAT 
GCCACAGAGT TGTTCAGTGA GGATGAAGAG AAGGCTCGGG AACAAGAAGA AGCAATAGTA 
GCACTGTGTG AGAATATCAC TGTTGCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

URB1 ( XM_012544405.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012399859.1 Sarcophilus harrisii URB1 ribosome biogenesis 1 homolog (S. cerevisiae) (URB1), mRNA.

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CloneID	OSa125228
Clone ID Related Accession (Same CDS sequence)	XM_012544405.3 , XM_012544405.2 , XM_012544405.1
Accession Version	XM_012544405.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	nucleolar pre-ribosomal-associated protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831550.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACC CAAAGAGGAA AGCCCTTAAG GAAGAGAGCA AGGCCTCCTC GGCCGGTCCT 
GCGAAAAGGC CTCGCGGGGA AGATTTCACC GGGCAGCAGT TTAAGACACA GCTCAAAGAC 
CCTCAGACTA TGGTGTCAGC TTTGGAAACT TTTATCTCCA TTGCCAAAAT GTTACCACAA 
ACTGGACTAT ATGATGTCGT GGAAGGATAT ATAAAAATTT CTGTTGAGTG CTCAGAGATT 
TTCCAACTTT TAAATGGAGA AAAAAGAACT GAACATGAAA GGATATTAAT TTTTAAAACT 
TTAGAGGCCA TATTACTGCG AACAGCAGGT GATCTTTCTC ATTTTCATGT TGTGGGAACC 
AACATCATGA AAAAAGTTAT GACTAGCCAT ATGAAGCTGA TTTATGATTC ACTAAATGCT 
TCAAGCTACA AAATGAGTCA AACCTGTTTG AACCTTATGT CCGCTATGGT GGCACAGGGT 
CCAGATGCTG CCAGGGATTT CTATAGCCGA TTTGACTTCA ACAAAAATTC ATTGTATGGT 
TTGGTGAAAA TAAGAAGTCC AAAGGGGCTG CCAGATGTTC GATTGGCATA TATCCAGTTT 
GCCCTTTCCT TCTTAATTGC TGGTGATGAT AATACCATAG CACGAGTGTT GGAGTTGAAA 
GAATTCCTCC CTGTCATTTT TGCGACAGGC TTGAAGGAAG ACAGAATTTC TACTATCAAT 
CTTTTGTTGT CCACCCTGAA AACCAAGGTG GTTCACAATA TTGGTGTTAC AAAGACTCAG 
AAAGTTCGTT TCTTCACTGG ATATTTGTTA AATAATGTGG CTTCTCTCTA TCGTTGGAAT 
GGAATTGCTG ATGTCAGCGC AGAAAATATC AAGCCTACTG AAGAAGCAGG CAAGACCATG 
GTAAGGGAAC TGGTTCACAA CTTCTTAATG GATCTTTGCT GTTCTCTGAA GCATGGCATT 
AACTTCTATG ATGCATCTTT TGGTACTCTG GGCAGAGGAG GGAACTTGAC TCTCTTGAAC 
TTCTTACTGG GTTTGAAGAC AGCAGCAGAT GATGACTTGG TGGCTGAACT TGTAGTGAAC 
ATCCTAAAAG TATGCCCAGA CTTACTTACG AAGTACTTTA AGGAGGTGAC CTATTCCTTT 
GTTCCAAGGA TGAAGTCGGC TTGGTTAAAC AATATCAGGC TACTAAGGAA GATTTATGAG 
GCTCAGCCAG AGATTTCCCC AGCATTTAAA ACTAAAGAGT TTATACCCTT GTCTAGATTG 
CTTTCCATGG TCATGGTGAC AACAGTACCC AACGTGTGCA ACAAAATCAT GTTTACTCAA 
GGATTAAAAT TGGAAAACAA ATCAGTAAAT CATACAATTT TATCACTTAT TTCCACCATT 
TTGAAGAGAG CATTAAAAAA CATTGATCAC TGTCTCAATA AGGATATATG GCAGGAGTCA 
AGCATCTATA CTCCTGTGAT GATGGAGGAA TTTGTGCATC TGTACAGAGA AGCTGTAAGC 
AAGCTTTTGC CAGATTTGAA CAATATTGTA TGTGTGTGGC AATCACTTGG AAAGCAAAAA 
ATAGATGAAG ATGGCGGGTT AGAAAAAAAA AATTCCCTTG AAGATGAGAA AACTGAAACT 
ACAGAAGTAG ACCACAAACA TGATGATGCA GAAACAACTC TTTTGAAGGC TATTTTACTT 
CAGGTCATTT GTCTTTATCA GATGGTTGTT CCCCATGTTG TCATTCAATG TACATTTGAC 
TTCAGCAAGC TTCTAAAAGG TATCATTTCA GAAGAGGGAT TTCAAGAGGA AGTTCCTCCT 
GTCCTTCAGT ACTATATTCT GAAAGTGGCC CTAGAATTGC CTGCCAGCAA ATTTGCCTGG 
TTAAAAGGAC AGGAAAGTCC AAATAGTGGA GAAAGATCTG TGTTTTATTT GCTGATGAAA 
ATGTTTGTGA CAAGTAAACA CTCACAACTT AAAGCATCAA CAAAACTATT GATTACAAAG 
ATTTTACGTG ACACTGGAGT ATTTGAATAT ACATGGAAAG AGCTAGAACT ATGGGTGGAG 
CAATTAGATA ACACAGCAGA AAACAGAAAA GAAGTTGTGA TTCAGTTTTT GGAATGTGTT 
TTGTTGCAAT TGGTAACAAA TCCCTATTCA TATACAGACA AGGCAGCAGA TTTTGTTCAA 
GAAGCTAGTA CACTACAGGC CACTGTGGTG AAGCAAGATG CTGATAATGT TAGCATACCT 
GTATCACACA TTGATGATAT CCTGGATATG GTGGATGTCC TCATGGAGGA CACTGAAGGC 
TTAGATGAGG AAGTTGGTTT CTCTTTAAAT GAAGATATGA TTCTGCTGAC ATTCCCATTC 
AGCGCCATAG TTCCTACAGC TCTGGAGAGC AGGAATAAAT TGCTTCTTGG GAATGGAAAT 
GATTCAGGTG AAAGCATAGT GGAATATCTG ATAGCCGTTC TGACAGACCT TCTACATACA 
CAGCGAGATC CTTTGGCTCT TTGTCTGATG CTTCAGTCTT ATGACAGTTT ACAACTTTCA 
AACACATCTT TGTATACTCG GATTTACCAG TTCAACAGAT ACTATAACCT GTGGATACCT 
GAGCAAGCCA GAGAAACTTT GGAATTTCAA GCTACCAGAC CCACTGAGAC ATCACTTGTG 
GTTGGCTCTG CCTTTTCCAC CTTGTTAACA GATACCTATG AGAGGGGAGT TACCCTCCTT 
TTAGAAGAGA ATATCCAGGG AAAACTGAGA GAAAAAATTG CTCAGCTCCC AATGCAGAAG 
GTTCTGCTCT CTGTGAAGCA GGTGATGCTG TATCTTAGAA CCACTGTGGA GAACTTTGCC 
AAGCTTGGTA AAAGTTCAGG TCCTGCTTTG CTTCAGCTCT TCATGAATTT GCTCAGTCAC 
CTGTTGAACC ATTGTTATCA TTTGGACACA TGTAACCAGA AAAAATGTGA AGCTGCTCAG 
GCCGAATCTG CTATCTTTTT GGATGAGGAG TCCTTGGCCA CACTGGAATT AGCCAATGAT 
AAGACTCTGG AAGATGCGTT GATAGTAATC TTTAGACATC CCACATTGGA GAGCTGGTAT 
TTGGCCCTGG AGCTGCAGAC TCTTCCCTCA CATAACCTCA ATCCAATCCT TGTAAAGCTT 
TTAGCAACTC ATTTCAATGC TGGGGTCCTC TCACTCCTGA AACTGAGTGC CCCAATTCTG 
CAGGAAATGC AACGTATGGA CATCCTTTCC AAGTATTTAG AAGCCATTAC TAAGAGTGTC 
TTGAAAGAAC TACACACCCT GAAGAAGACA GATCCTCCCA TTGCATATGG GAAACCTTCA 
GCTCAGCTGG AAGCTTTGTA CCAACTACAT CCCTACATGG ATGGAACCCA GCTTAGGGAC 
ATTACGCTTG CACTCCTGGC TCTTCCTAAA TCAACCCTTT TGGATCAGGA ATCTAAGAAA 
TCTCACCAAA AAAAAAAACA TCTGAGCTTT TTAGGGGAAA CCCTCAAACA GCTTATCACT 
GAATCCTACC AGGAACAATC CCAGCAAGGT GAATTACTGT CGTCCCTAGA ATATGTGAAG 
AGAATGAGTG TCCTGTTGCC TGCACTGGTC ATTGAAGAGT TAGAGACTGT GTTCTTCCAA 
GCTTTACAGA AAGAGCCAAT TCTGGTCCAT GTAGTTGAGT TAGATTTATT GACCTACTGT 
CTAAATCGGA GGACAAAAAC TTCTCTTTCC ATTGTAGCTT TGTTGATTCA GCAAAGTAGT 
ACTCACCTGC TACACTTTGA GTTGTGGTGC TTACAGGGCC TAGGGTCCTG CCTTCCAGAA 
AACTTCAGCC TCTTTCTTCC ACTTATCCAT ACCTATTTAC AGTCTTGTAT GCAGGGAGAC 
TTTACACGTC CTTCTAAAGT GTCTAGAAAT GTCATGAATG TGTTGAGAAA GGTCCTGTGG 
AAAGAGCTTC AAAACAGTCT TTTAGGAGAT GATCCTCAGG ATCTTGGAAT TCAAGAAGAG 
GTCCTTTCTA AATTGATTCC CTATTCAAGA GCAAAAGAAC TTAGACTCCT CATTGACAAA 
ATGCCCAGCA TTCTTCAAAA GCCAGAGAAA TCGAAGAGCT GGATTGTATC AGATGCCATA 
GCATTAGTAT TGGAAAAATC TGTGGAAGAG CTGGATGTTT GGAAAAAGAC TTTGTTGGGG 
TCATGTATAA AATGGCTAAT AGCTTCATAC AGCAGTAGTA TCAAAGAAGA AAATACCCAG 
GATGCAGAGA AGTCTATGTT GTTGCGATTT AATGAATTAT TGAACTCAGT TAATGAAGTT 
GATCCTGTTG ATTGGCAGAA GTTTGTGAAG ACAGGCCTTA AGTTCCGCTA CCATGATTAC 
ACATTTTTAA AGACTCTTCA TGCTTGTATA CTGCTGTTAT ATCAGCCTGA AAATTCCCTT 
TGCAAAAACT TAGTGCAGCT GCCACTAATA TATGTGATGA TCACACAACA CTCTATGTTT 
TTGCCAACCA TATTGCAAAC AAAAGAAGAT GACACAAATA TCCAAGTAAA AGAAATGTTA 
GTGGACCTAA TATCAACTAT TGTAAAACTT TGCCCTTCCG TCTGTGAAAG CAACCACTTT 
GCAGTGCTAC TAGGAGGCTA TGGTGCAACC CTCAGTGTTC TAGATCAGAA GATATTGCTG 
CTTCTTAGAT TGTATGAAAG AAATGATCTG AGCCTCATAA ACTTCAGAAT GCTGCTATGG 
GGCCCTGCTG CTGTGGAACA TCACAAGACG TGTAAGAGCC TGGGAAAGTC ACTATGGCAG 
CAGCCAAGTG TGGGAGAAAT CTTATGTCTC CTGGATCGAG AGAGAATGAT GAAGACCATT 
CTTCACTTCC CACAGAATCG AAAACTTCTG CTAAAGGAGG ATGCACAGGA TATATTCAAA 
GACAAAAGCA AAGTAGACTT CGACGAACTT TATGATCCCT GTTTTCTACT TCACCTTTTC 
AGTGAACTTA CCAGACCAGA ATTTATAGTG GATTGTCACA AATTTGTTGA TTCCAATGCC 
CTGGGCCTCA CAGTTTCTGC CCTTAGCAGT TACGATCCTC AGATGCGAGC AGCAGCTTAT 
TATGTTCTGG CTTCCTATTA TTCCCATTTG GAAGGGGCTC GATTTCGAGA ACAGCCACAG 
CTGCTCTATC TCTTGGACAT ATTACAAAAT GGAATCAGAA AACAGAATCT CAGATTGACC 
TATACCTTGA CACTGTTTAT TGCCAAAACT GCCTTGCAGA TTCTGAAACC AGAGGAGCAC 
ATGTACATGA AGATAAACAA ATTCCTTTTG TCACATCAGG ACTTATACTT GAACAAAGTA 
CCGGGATTCT ATAACTTTTT CTACAGCTCT GATTCAGAGT ATAGGATAGA ACAGGAGTGG 
ATATTTGAAC TTTTGAGACA AGGAATTCGA GACACACACT GCTATGATTT GTATTCCTCT 
CAGAGAATTT TCCATATCAT CCTGTCCTTC TTCAACAGCC CATTATGTGA TGAAACAATG 
CAGAACTGGA TTATAGAAAT CTTACAAAAT GCTGCCCAGA TTACCAAAGC TGCCTTTGAA 
ATTGTGCAGG AACATAGCCT TTTAACTTGG ATTTTGCATA TCCTTGGGAA AAAGTTTTTG 
GAAAACCAGA TGCTATCCAA CATTATATGC TTGCTGCATA CTCTCTGGAT ATCCAACTTG 
GGAAACAAAA GAATGGAAAT ACAGGAAGAC CATCCTTGCG AGCTTGTGGC TACACAGTCA 
CCAAAGCTGC TTCCTCTACA ATTTGTCAAT GAATTCCTCT ATGTTTTGGT TGTGTTGGCG 
AAATATCTTC GGCCCACCTT GGACTCTATC CAGATCACTC ACTATTTTAG TACTTTGAGC 
TCTGTGCTGA GGTACCGAGA AACTGTTTTA GAAGCCTTCA AGGAAATGGA CCGATTGACT 
GTAAATGAAA ATGTATTTTC TACCAAAGAT GTCCTCATGC TCTTACATAA GTGGAGCATA 
ATTATGGGAG ATGTTAAACT CCAAGAAGAC TTGAAAACAG TGATTGAAAA ATACCAAGTC 
AAGGAATTAA TGAAAATTAT TAAAGATAGG AACAAACCCC TTGCACCAGC TCGATCTAAA 
GCTCAGCGTA ACAAAAAAAA GATGGAGGTG GATCCAGAGG AAACAATTGA TCCAGAGTTA 
AAGGCTTCCA ACCTGATGAA ATGCCGAGGT CTCCTGAGAT CCATACTGAC CCACTGGGAC 
CCCGCCTTTG CTGTGGTAGA GACAGAGCCA AAGCAAGAAC GAGTCTCCTT AGCTCCTGAC 
CCAGAGAGAG AAGCTGTGGA TCTTGTTGGG GCCACCGCTG TTCTGGTGGC CCAATGGGAG 
ATCAGCTCCC TCATGGACCC AGCGCTGGAT GTCCAGGGAA TTCCAGAGGT CCTCAGGTGG 
TTTAAAAGCA GCATTTTGCT TCATCCTGTG GTTGTCTCAG AACTTGTCAG AGATGTTACA 
TGGAGAAATA GCATCTTTAA GCTGTACAGC CAGCTTTCCC ATGCCAGAGA ACTGAAAGCA 
GCCTCTCAGA GCGTCTTCTG CCTCTTCAAT ACAGTCATGC TTCACTTAAT GGCATCCCAG 
GGCCCAATAG AGAACTCTTT TCATGAAGTG GTAGAAACAA TCGCTCTTTC TTCTACAGAT 
GAAGAAGACA AAACAAAACA AGCTGCTGCA GTGTTCCTGG TATCTCTTTA TATTAAAGAT 
ATCTGGCTAG GGGCAAAGCC AGTGAATACC TTCCTGACTC ACATCCGAAT GATCTGTGAT 
GCCACAGAGT TGTTCAGTGA GGATGAAGAG AAGGCTCGGG AACAAGAAGA AGCAATAGTA 
GCACTGTGTG AGAATATCAC TGTTGCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012399859.1
CDS	1..6813
Translation	MGNPKRKALKEESKASSAGPAKRPRGEDFTGQQFKTQLKDPQTMVSALETFISIAKMLPQTGLYDVVEGYIKISVECSEIFQLLNGEKRTEHERILIFKTLEAILLRTAGDLSHFHVVGTNIMKKVMTSHMKLIYDSLNASSYKMSQTCLNLMSAMVAQGPDAARDFYSRFDFNKNSLYGLVKIRSPKGLPDVRLAYIQFALSFLIAGDDNTIARVLELKEFLPVIFATGLKEDRISTINLLLSTLKTKVVHNIGVTKTQKVRFFTGYLLNNVASLYRWNGIADVSAENIKPTEEAGKTMVRELVHNFLMDLCCSLKHGINFYDASFGTLGRGGNLTLLNFLLGLKTAADDDLVAELVVNILKVCPDLLTKYFKEVTYSFVPRMKSAWLNNIRLLRKIYEAQPEISPAFKTKEFIPLSRLLSMVMVTTVPNVCNKIMFTQGLKLENKSVNHTILSLISTILKRALKNIDHCLNKDIWQESSIYTPVMMEEFVHLYREAVSKLLPDLNNIVCVWQSLGKQKIDEDGGLEKKNSLEDEKTETTEVDHKHDDAETTLLKAILLQVICLYQMVVPHVVIQCTFDFSKLLKGIISEEGFQEEVPPVLQYYILKVALELPASKFAWLKGQESPNSGERSVFYLLMKMFVTSKHSQLKASTKLLITKILRDTGVFEYTWKELELWVEQLDNTAENRKEVVIQFLECVLLQLVTNPYSYTDKAADFVQEASTLQATVVKQDADNVSIPVSHIDDILDMVDVLMEDTEGLDEEVGFSLNEDMILLTFPFSAIVPTALESRNKLLLGNGNDSGESIVEYLIAVLTDLLHTQRDPLALCLMLQSYDSLQLSNTSLYTRIYQFNRYYNLWIPEQARETLEFQATRPTETSLVVGSAFSTLLTDTYERGVTLLLEENIQGKLREKIAQLPMQKVLLSVKQVMLYLRTTVENFAKLGKSSGPALLQLFMNLLSHLLNHCYHLDTCNQKKCEAAQAESAIFLDEESLATLELANDKTLEDALIVIFRHPTLESWYLALELQTLPSHNLNPILVKLLATHFNAGVLSLLKLSAPILQEMQRMDILSKYLEAITKSVLKELHTLKKTDPPIAYGKPSAQLEALYQLHPYMDGTQLRDITLALLALPKSTLLDQESKKSHQKKKHLSFLGETLKQLITESYQEQSQQGELLSSLEYVKRMSVLLPALVIEELETVFFQALQKEPILVHVVELDLLTYCLNRRTKTSLSIVALLIQQSSTHLLHFELWCLQGLGSCLPENFSLFLPLIHTYLQSCMQGDFTRPSKVSRNVMNVLRKVLWKELQNSLLGDDPQDLGIQEEVLSKLIPYSRAKELRLLIDKMPSILQKPEKSKSWIVSDAIALVLEKSVEELDVWKKTLLGSCIKWLIASYSSSIKEENTQDAEKSMLLRFNELLNSVNEVDPVDWQKFVKTGLKFRYHDYTFLKTLHACILLLYQPENSLCKNLVQLPLIYVMITQHSMFLPTILQTKEDDTNIQVKEMLVDLISTIVKLCPSVCESNHFAVLLGGYGATLSVLDQKILLLLRLYERNDLSLINFRMLLWGPAAVEHHKTCKSLGKSLWQQPSVGEILCLLDRERMMKTILHFPQNRKLLLKEDAQDIFKDKSKVDFDELYDPCFLLHLFSELTRPEFIVDCHKFVDSNALGLTVSALSSYDPQMRAAAYYVLASYYSHLEGARFREQPQLLYLLDILQNGIRKQNLRLTYTLTLFIAKTALQILKPEEHMYMKINKFLLSHQDLYLNKVPGFYNFFYSSDSEYRIEQEWIFELLRQGIRDTHCYDLYSSQRIFHIILSFFNSPLCDETMQNWIIEILQNAAQITKAAFEIVQEHSLLTWILHILGKKFLENQMLSNIICLLHTLWISNLGNKRMEIQEDHPCELVATQSPKLLPLQFVNEFLYVLVVLAKYLRPTLDSIQITHYFSTLSSVLRYRETVLEAFKEMDRLTVNENVFSTKDVLMLLHKWSIIMGDVKLQEDLKTVIEKYQVKELMKIIKDRNKPLAPARSKAQRNKKKMEVDPEETIDPELKASNLMKCRGLLRSILTHWDPAFAVVETEPKQERVSLAPDPEREAVDLVGATAVLVAQWEISSLMDPALDVQGIPEVLRWFKSSILLHPVVVSELVRDVTWRNSIFKLYSQLSHARELKAASQSVFCLFNTVMLHLMASQGPIENSFHEVVETIALSSTDEEDKTKQAAAVFLVSLYIKDIWLGAKPVNTFLTHIRMICDATELFSEDEEKAREQEEAIVALCENITVAT

Target ORF information:

RefSeq Version	XM_012544405.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii URB1 ribosome biogenesis 1 homolog (S. cerevisiae) (URB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012544405.1

ORF Insert Sequence:

ATGGGAAACC CAAAGAGGAA AGCCCTTAAG GAAGAGAGCA AGGCCTCCTC GGCCGGTCCT 
GCGAAAAGGC CTCGCGGGGA AGATTTCACC GGGCAGCAGT TTAAGACACA GCTCAAAGAC 
CCTCAGACTA TGGTGTCAGC TTTGGAAACT TTTATCTCCA TTGCCAAAAT GTTACCACAA 
ACTGGACTAT ATGATGTCGT GGAAGGATAT ATAAAAATTT CTGTTGAGTG CTCAGAGATT 
TTCCAACTTT TAAATGGAGA AAAAAGAACT GAACATGAAA GGATATTAAT TTTTAAAACT 
TTAGAGGCCA TATTACTGCG AACAGCAGGT GATCTTTCTC ATTTTCATGT TGTGGGAACC 
AACATCATGA AAAAAGTTAT GACTAGCCAT ATGAAGCTGA TTTATGATTC ACTAAATGCT 
TCAAGCTACA AAATGAGTCA AACCTGTTTG AACCTTATGT CCGCTATGGT GGCACAGGGT 
CCAGATGCTG CCAGGGATTT CTATAGCCGA TTTGACTTCA ACAAAAATTC ATTGTATGGT 
TTGGTGAAAA TAAGAAGTCC AAAGGGGCTG CCAGATGTTC GATTGGCATA TATCCAGTTT 
GCCCTTTCCT TCTTAATTGC TGGTGATGAT AATACCATAG CACGAGTGTT GGAGTTGAAA 
GAATTCCTCC CTGTCATTTT TGCGACAGGC TTGAAGGAAG ACAGAATTTC TACTATCAAT 
CTTTTGTTGT CCACCCTGAA AACCAAGGTG GTTCACAATA TTGGTGTTAC AAAGACTCAG 
AAAGTTCGTT TCTTCACTGG ATATTTGTTA AATAATGTGG CTTCTCTCTA TCGTTGGAAT 
GGAATTGCTG ATGTCAGCGC AGAAAATATC AAGCCTACTG AAGAAGCAGG CAAGACCATG 
GTAAGGGAAC TGGTTCACAA CTTCTTAATG GATCTTTGCT GTTCTCTGAA GCATGGCATT 
AACTTCTATG ATGCATCTTT TGGTACTCTG GGCAGAGGAG GGAACTTGAC TCTCTTGAAC 
TTCTTACTGG GTTTGAAGAC AGCAGCAGAT GATGACTTGG TGGCTGAACT TGTAGTGAAC 
ATCCTAAAAG TATGCCCAGA CTTACTTACG AAGTACTTTA AGGAGGTGAC CTATTCCTTT 
GTTCCAAGGA TGAAGTCGGC TTGGTTAAAC AATATCAGGC TACTAAGGAA GATTTATGAG 
GCTCAGCCAG AGATTTCCCC AGCATTTAAA ACTAAAGAGT TTATACCCTT GTCTAGATTG 
CTTTCCATGG TCATGGTGAC AACAGTACCC AACGTGTGCA ACAAAATCAT GTTTACTCAA 
GGATTAAAAT TGGAAAACAA ATCAGTAAAT CATACAATTT TATCACTTAT TTCCACCATT 
TTGAAGAGAG CATTAAAAAA CATTGATCAC TGTCTCAATA AGGATATATG GCAGGAGTCA 
AGCATCTATA CTCCTGTGAT GATGGAGGAA TTTGTGCATC TGTACAGAGA AGCTGTAAGC 
AAGCTTTTGC CAGATTTGAA CAATATTGTA TGTGTGTGGC AATCACTTGG AAAGCAAAAA 
ATAGATGAAG ATGGCGGGTT AGAAAAAAAA AATTCCCTTG AAGATGAGAA AACTGAAACT 
ACAGAAGTAG ACCACAAACA TGATGATGCA GAAACAACTC TTTTGAAGGC TATTTTACTT 
CAGGTCATTT GTCTTTATCA GATGGTTGTT CCCCATGTTG TCATTCAATG TACATTTGAC 
TTCAGCAAGC TTCTAAAAGG TATCATTTCA GAAGAGGGAT TTCAAGAGGA AGTTCCTCCT 
GTCCTTCAGT ACTATATTCT GAAAGTGGCC CTAGAATTGC CTGCCAGCAA ATTTGCCTGG 
TTAAAAGGAC AGGAAAGTCC AAATAGTGGA GAAAGATCTG TGTTTTATTT GCTGATGAAA 
ATGTTTGTGA CAAGTAAACA CTCACAACTT AAAGCATCAA CAAAACTATT GATTACAAAG 
ATTTTACGTG ACACTGGAGT ATTTGAATAT ACATGGAAAG AGCTAGAACT ATGGGTGGAG 
CAATTAGATA ACACAGCAGA AAACAGAAAA GAAGTTGTGA TTCAGTTTTT GGAATGTGTT 
TTGTTGCAAT TGGTAACAAA TCCCTATTCA TATACAGACA AGGCAGCAGA TTTTGTTCAA 
GAAGCTAGTA CACTACAGGC CACTGTGGTG AAGCAAGATG CTGATAATGT TAGCATACCT 
GTATCACACA TTGATGATAT CCTGGATATG GTGGATGTCC TCATGGAGGA CACTGAAGGC 
TTAGATGAGG AAGTTGGTTT CTCTTTAAAT GAAGATATGA TTCTGCTGAC ATTCCCATTC 
AGCGCCATAG TTCCTACAGC TCTGGAGAGC AGGAATAAAT TGCTTCTTGG GAATGGAAAT 
GATTCAGGTG AAAGCATAGT GGAATATCTG ATAGCCGTTC TGACAGACCT TCTACATACA 
CAGCGAGATC CTTTGGCTCT TTGTCTGATG CTTCAGTCTT ATGACAGTTT ACAACTTTCA 
AACACATCTT TGTATACTCG GATTTACCAG TTCAACAGAT ACTATAACCT GTGGATACCT 
GAGCAAGCCA GAGAAACTTT GGAATTTCAA GCTACCAGAC CCACTGAGAC ATCACTTGTG 
GTTGGCTCTG CCTTTTCCAC CTTGTTAACA GATACCTATG AGAGGGGAGT TACCCTCCTT 
TTAGAAGAGA ATATCCAGGG AAAACTGAGA GAAAAAATTG CTCAGCTCCC AATGCAGAAG 
GTTCTGCTCT CTGTGAAGCA GGTGATGCTG TATCTTAGAA CCACTGTGGA GAACTTTGCC 
AAGCTTGGTA AAAGTTCAGG TCCTGCTTTG CTTCAGCTCT TCATGAATTT GCTCAGTCAC 
CTGTTGAACC ATTGTTATCA TTTGGACACA TGTAACCAGA AAAAATGTGA AGCTGCTCAG 
GCCGAATCTG CTATCTTTTT GGATGAGGAG TCCTTGGCCA CACTGGAATT AGCCAATGAT 
AAGACTCTGG AAGATGCGTT GATAGTAATC TTTAGACATC CCACATTGGA GAGCTGGTAT 
TTGGCCCTGG AGCTGCAGAC TCTTCCCTCA CATAACCTCA ATCCAATCCT TGTAAAGCTT 
TTAGCAACTC ATTTCAATGC TGGGGTCCTC TCACTCCTGA AACTGAGTGC CCCAATTCTG 
CAGGAAATGC AACGTATGGA CATCCTTTCC AAGTATTTAG AAGCCATTAC TAAGAGTGTC 
TTGAAAGAAC TACACACCCT GAAGAAGACA GATCCTCCCA TTGCATATGG GAAACCTTCA 
GCTCAGCTGG AAGCTTTGTA CCAACTACAT CCCTACATGG ATGGAACCCA GCTTAGGGAC 
ATTACGCTTG CACTCCTGGC TCTTCCTAAA TCAACCCTTT TGGATCAGGA ATCTAAGAAA 
TCTCACCAAA AAAAAAAACA TCTGAGCTTT TTAGGGGAAA CCCTCAAACA GCTTATCACT 
GAATCCTACC AGGAACAATC CCAGCAAGGT GAATTACTGT CGTCCCTAGA ATATGTGAAG 
AGAATGAGTG TCCTGTTGCC TGCACTGGTC ATTGAAGAGT TAGAGACTGT GTTCTTCCAA 
GCTTTACAGA AAGAGCCAAT TCTGGTCCAT GTAGTTGAGT TAGATTTATT GACCTACTGT 
CTAAATCGGA GGACAAAAAC TTCTCTTTCC ATTGTAGCTT TGTTGATTCA GCAAAGTAGT 
ACTCACCTGC TACACTTTGA GTTGTGGTGC TTACAGGGCC TAGGGTCCTG CCTTCCAGAA 
AACTTCAGCC TCTTTCTTCC ACTTATCCAT ACCTATTTAC AGTCTTGTAT GCAGGGAGAC 
TTTACACGTC CTTCTAAAGT GTCTAGAAAT GTCATGAATG TGTTGAGAAA GGTCCTGTGG 
AAAGAGCTTC AAAACAGTCT TTTAGGAGAT GATCCTCAGG ATCTTGGAAT TCAAGAAGAG 
GTCCTTTCTA AATTGATTCC CTATTCAAGA GCAAAAGAAC TTAGACTCCT CATTGACAAA 
ATGCCCAGCA TTCTTCAAAA GCCAGAGAAA TCGAAGAGCT GGATTGTATC AGATGCCATA 
GCATTAGTAT TGGAAAAATC TGTGGAAGAG CTGGATGTTT GGAAAAAGAC TTTGTTGGGG 
TCATGTATAA AATGGCTAAT AGCTTCATAC AGCAGTAGTA TCAAAGAAGA AAATACCCAG 
GATGCAGAGA AGTCTATGTT GTTGCGATTT AATGAATTAT TGAACTCAGT TAATGAAGTT 
GATCCTGTTG ATTGGCAGAA GTTTGTGAAG ACAGGCCTTA AGTTCCGCTA CCATGATTAC 
ACATTTTTAA AGACTCTTCA TGCTTGTATA CTGCTGTTAT ATCAGCCTGA AAATTCCCTT 
TGCAAAAACT TAGTGCAGCT GCCACTAATA TATGTGATGA TCACACAACA CTCTATGTTT 
TTGCCAACCA TATTGCAAAC AAAAGAAGAT GACACAAATA TCCAAGTAAA AGAAATGTTA 
GTGGACCTAA TATCAACTAT TGTAAAACTT TGCCCTTCCG TCTGTGAAAG CAACCACTTT 
GCAGTGCTAC TAGGAGGCTA TGGTGCAACC CTCAGTGTTC TAGATCAGAA GATATTGCTG 
CTTCTTAGAT TGTATGAAAG AAATGATCTG AGCCTCATAA ACTTCAGAAT GCTGCTATGG 
GGCCCTGCTG CTGTGGAACA TCACAAGACG TGTAAGAGCC TGGGAAAGTC ACTATGGCAG 
CAGCCAAGTG TGGGAGAAAT CTTATGTCTC CTGGATCGAG AGAGAATGAT GAAGACCATT 
CTTCACTTCC CACAGAATCG AAAACTTCTG CTAAAGGAGG ATGCACAGGA TATATTCAAA 
GACAAAAGCA AAGTAGACTT CGACGAACTT TATGATCCCT GTTTTCTACT TCACCTTTTC 
AGTGAACTTA CCAGACCAGA ATTTATAGTG GATTGTCACA AATTTGTTGA TTCCAATGCC 
CTGGGCCTCA CAGTTTCTGC CCTTAGCAGT TACGATCCTC AGATGCGAGC AGCAGCTTAT 
TATGTTCTGG CTTCCTATTA TTCCCATTTG GAAGGGGCTC GATTTCGAGA ACAGCCACAG 
CTGCTCTATC TCTTGGACAT ATTACAAAAT GGAATCAGAA AACAGAATCT CAGATTGACC 
TATACCTTGA CACTGTTTAT TGCCAAAACT GCCTTGCAGA TTCTGAAACC AGAGGAGCAC 
ATGTACATGA AGATAAACAA ATTCCTTTTG TCACATCAGG ACTTATACTT GAACAAAGTA 
CCGGGATTCT ATAACTTTTT CTACAGCTCT GATTCAGAGT ATAGGATAGA ACAGGAGTGG 
ATATTTGAAC TTTTGAGACA AGGAATTCGA GACACACACT GCTATGATTT GTATTCCTCT 
CAGAGAATTT TCCATATCAT CCTGTCCTTC TTCAACAGCC CATTATGTGA TGAAACAATG 
CAGAACTGGA TTATAGAAAT CTTACAAAAT GCTGCCCAGA TTACCAAAGC TGCCTTTGAA 
ATTGTGCAGG AACATAGCCT TTTAACTTGG ATTTTGCATA TCCTTGGGAA AAAGTTTTTG 
GAAAACCAGA TGCTATCCAA CATTATATGC TTGCTGCATA CTCTCTGGAT ATCCAACTTG 
GGAAACAAAA GAATGGAAAT ACAGGAAGAC CATCCTTGCG AGCTTGTGGC TACACAGTCA 
CCAAAGCTGC TTCCTCTACA ATTTGTCAAT GAATTCCTCT ATGTTTTGGT TGTGTTGGCG 
AAATATCTTC GGCCCACCTT GGACTCTATC CAGATCACTC ACTATTTTAG TACTTTGAGC 
TCTGTGCTGA GGTACCGAGA AACTGTTTTA GAAGCCTTCA AGGAAATGGA CCGATTGACT 
GTAAATGAAA ATGTATTTTC TACCAAAGAT GTCCTCATGC TCTTACATAA GTGGAGCATA 
ATTATGGGAG ATGTTAAACT CCAAGAAGAC TTGAAAACAG TGATTGAAAA ATACCAAGTC 
AAGGAATTAA TGAAAATTAT TAAAGATAGG AACAAACCCC TTGCACCAGC TCGATCTAAA 
GCTCAGCGTA ACAAAAAAAA GATGGAGGTG GATCCAGAGG AAACAATTGA TCCAGAGTTA 
AAGGCTTCCA ACCTGATGAA ATGCCGAGGT CTCCTGAGAT CCATACTGAC CCACTGGGAC 
CCCGCCTTTG CTGTGGTAGA GACAGAGCCA AAGCAAGAAC GAGTCTCCTT AGCTCCTGAC 
CCAGAGAGAG AAGCTGTGGA TCTTGTTGGG GCCACCGCTG TTCTGGTGGC CCAATGGGAG 
ATCAGCTCCC TCATGGACCC AGCGCTGGAT GTCCAGGGAA TTCCAGAGGT CCTCAGGTGG 
TTTAAAAGCA GCATTTTGCT TCATCCTGTG GTTGTCTCAG AACTTGTCAG AGATGTTACA 
TGGAGAAATA GCATCTTTAA GCTGTACAGC CAGCTTTCCC ATGCCAGAGA ACTGAAAGCA 
GCCTCTCAGA GCGTCTTCTG CCTCTTCAAT ACAGTCATGC TTCACTTAAT GGCATCCCAG 
GGCCCAATAG AGAACTCTTT TCATGAAGTG GTAGAAACAA TCGCTCTTTC TTCTACAGAT 
GAAGAAGACA AAACAAAACA AGCTGCTGCA GTGTTCCTGG TATCTCTTTA TATTAAAGAT 
ATCTGGCTAG GGGCAAAGCC AGTGAATACC TTCCTGACTC ACATCCGAAT GATCTGTGAT 
GCCACAGAGT TGTTCAGTGA GGATGAAGAG AAGGCTCGGG AACAAGAAGA AGCAATAGTA 
GCACTGTGTG AGAATATCAC TGTTGCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

URB1 ( XM_023498904.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023354672.1 Sarcophilus harrisii URB1 ribosome biogenesis 1 homolog (S. cerevisiae) (URB1), transcript variant X2, mRNA.

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CloneID	OSa151555
Clone ID Related Accession (Same CDS sequence)	XM_023498904.1 , XM_023498904.2
Accession Version	XM_023498904.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	nucleolar pre-ribosomal-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831550.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACC CAAAGAGGAA AGCCCTTAAG GAAGAGAGCA AGGCCTCCTC GGCCGGTCCT 
GCGAAAAGGC CTCGCGGGGA AGATTTCACC GGGCAGCAGT TTAAGACACA GCTCAAAGAC 
CCTCAGACTA TGGTGTCAGC TTTGGAAACT TTTATCTCCA TTGCCAAAAT GTTACCACAA 
ACTGGACTAT ATGATGTCGT GGAAGGATAT ATAAAAATTT CTGTTGAGTG CTCAGAGATT 
TTCCAACTTT TAAATGGAGA AAAAAGAACT GAACATGAAA GGATATTAAT TTTTAAAACT 
TTAGAGGCCA TATTACTGCG AACAGCAGGT GATCTTTCTC ATTTTCATGT TGTGGGAACC 
AACATCATGA AAAAAGTTAT GACTAGCCAT ATGAAGCTGA TTTATGATTC ACTAAATGCT 
TCAAGCTACA AAATGAGTCA AACCTGTTTG AACCTTATGT CCGCTATGGT GGCACAGGGT 
CCAGATGCTG CCAGGGATTT CTATAGCCGA TTTGACTTCA ACAAAAATTC ATTGTATGGT 
TTGGTGAAAA TAAGAAGTCC AAAGGGGCTG CCAGATGTTC GATTGGCATA TATCCAGTTT 
GCCCTTTCCT TCTTAATTGC TGGTGATGAT AATACCATAG CACGAGTGTT GGAGTTGAAA 
GAATTCCTCC CTGTCATTTT TGCGACAGGC TTGAAGGAAG ACAGAATTTC TACTATCAAT 
CTTTTGTTGT CCACCCTGAA AACCAAGGTG GTTCACAATA TTGGTGTTAC AAAGACTCAG 
AAAGTTCGTT TCTTCACTGG ATATTTGTTA AATAATGTGG CTTCTCTCTA TCGTTGGAAT 
GGAATTGCTG ATGTCAGCGC AGAAAATATC AAGCCTACTG AAGAAGCAGG CAAGACCATG 
GTAAGGGAAC TGGTTCACAA CTTCTTAATG GATCTTTGCT GTTCTCTGAA GCATGGCATT 
AACTTCTATG ATGCATCTTT TGGTACTCTG GGCAGAGGAG GGAACTTGAC TCTCTTGAAC 
TTCTTACTGG GTTTGAAGAC AGCAGCAGAT GATGACTTGG TGGCTGAACT TGTAGTGAAC 
ATCCTAAAAG TATGCCCAGA CTTACTTACG AAGTACTTTA AGGAGGTGAC CTATTCCTTT 
GTTCCAAGGA TGAAGTCGGC TTGGTTAAAC AATATCAGGC TACTAAGGAA GATTTATGAG 
GCTCAGCCAG AGATTTCCCC AGCATTTAAA ACTAAAGAGT TTATACCCTT GTCTAGATTG 
CTTTCCATGG TCATGGTGAC AACAGTACCC AACGTGTGCA ACAAAATCAT GTTTACTCAA 
GGATTAAAAT TGGAAAACAA ATCAGTAAAT CATACAATTT TATCACTTAT TTCCACCATT 
TTGAAGAGAG CATTAAAAAA CATTGATCAC TGTCTCAATA AGGATATATG GCAGGAGTCA 
AGCATCTATA CTCCTGTGAT GATGGAGGAA TTTGTGCATC TGTACAGAGA AGCTGTAAGC 
AAGCTTTTGC CAGATTTGAA CAATATTGTA TGTGTGTGGC AATCACTTGG AAAGCAAAAA 
ATAGATGAAG ATGGCGGGTT AGAAAAAAAA AATTCCCTTG AAGATGAGAA AACTGAAACT 
ACAGAAGTAG ACCACAAACA TGATGATGCA GAAACAACTC TTTTGAAGGC TATTTTACTT 
CAGGTCATTT GTCTTTATCA GATGGTTGTT CCCCATGTTG TCATTCAATG TACATTTGAC 
TTCAGCAAGC TTCTAAAAGG TATCATTTCA GAAGAGGGAT TTCAAGAGGA AGTTCCTCCT 
GTCCTTCAGT ACTATATTCT GAAAGTGGCC CTAGAATTGC CTGCCAGCAA ATTTGCCTGG 
TTAAAAGGAC AGGAAAGTCC AAATAGTGGA GAAAGATCTG TGTTTTATTT GCTGATGAAA 
ATGTTTGTGA CAAGTAAACA CTCACAACTT AAAGCATCAA CAAAACTATT GATTACAAAG 
ATTTTACGTG ACACTGGAGT ATTTGAATAT ACATGGAAAG AGCTAGAACT ATGGGTGGAG 
CAATTAGATA ACACAGCAGA AAACAGAAAA GAAGTTGTGA TTCAGTTTTT GGAATGTGTT 
TTGTTGCAAT TGGTAACAAA TCCCTATTCA TATACAGACA AGGCAGCAGA TTTTGTTCAA 
GAAGCTAGTA CACTACAGGC CACTGTGGTG AAGCAAGATG CTGATAATGT TAGCATACCT 
GTATCACACA TTGATGATAT CCTGGATATG GTGGATGTCC TCATGGAGGA CACTGAAGGC 
TTAGATGAGG AAGTTGGTTT CTCTTTAAAT GAAGATATGA TTCTGCTGAC ATTCCCATTC 
AGCGCCATAG TTCCTACAGC TCTGGAGAGC AGGAATAAAT TGCTTCTTGG GAATGGAAAT 
GATTCAGGTG AAAGCATAGT GGAATATCTG ATAGCCGTTC TGACAGACCT TCTACATACA 
CAGCGAGATC CTTTGGCTCT TTGTCTGATG CTTCAGTCTT ATGACAGTTT ACAACTTTCA 
AACACATCTT TGTATACTCG GATTTACCAG TTCAACAGAT ACTATAACCT GTGGATACCT 
GAGCAAGCCA GAGAAACTTT GGAATTTCAA GCTACCAGAC CCACTGAGAC ATCACTTGTG 
GTTGGCTCTG CCTTTTCCAC CTTGTTAACA GATACCTATG AGAGGGGAGT TACCCTCCTT 
TTAGAAGAGA ATATCCAGGG AAAACTGAGA GAAAAAATTG CTCAGCTCCC AATGCAGAAG 
GTTCTGCTCT CTGTGAAGCA GGTGATGCTG TATCTTAGAA CCACTGTGGA GAACTTTGCC 
AAGCTTGGTA AAAGTTCAGG TCCTGCTTTG CTTCAGCTCT TCATGAATTT GCTCAGTCAC 
CTGTTGAACC ATTGTTATCA TTTGGACACA TGTAACCAGA AAAAATGTGA AGCTGCTCAG 
GCCGAATCTG CTATCTTTTT GGATGAGGAG TCCTTGGCCA CACTGGAATT AGCCAATGAT 
AAGACTCTGG AAGATGCGTT GATAGTAATC TTTAGACATC CCACATTGGA GAGCTGGTAT 
TTGGCCCTGG AGCTGCAGAC TCTTCCCTCA CATAACCTCA ATCCAATCCT TGTAAAGCTT 
TTAGCAACTC ATTTCAATGC TGGGGTCCTC TCACTCCTGA AACTGAGTGC CCCAATTCTG 
CAGGAAATGC AACGTATGGA CATCCTTTCC AAGTATTTAG AAGCCATTAC TAAGAGTGTC 
TTGAAAGAAC TACACACCCT GAAGAAGACA GATCCTCCCA TTGCATATGG GAAACCTTCA 
GCTCAGCTGG AAGCTTTGTA CCAACTACAT CCCTACATGG ATGGAACCCA GCTTAGGGAC 
ATTACGCTTG CACTCCTGGC TCTTCCTAAA TCAACCCTTT TGGATCAGGA ATCTAAGAAA 
TCTCACCAAA AAAAAAAACA TCTGAGCTTT TTAGGGGAAA CCCTCAAACA GCTTATCACT 
GAATCCTACC AGGAACAATC CCAGCAAGGT GAATTACTGT CGTCCCTAGA ATATGTGAAG 
AGAATGAGTG TCCTGTTGCC TGCACTGGTC ATTGAAGAGT TAGAGACTGT GTTCTTCCAA 
GCTTTACAGA AAGAGCCAAT TCTGGTCCAT GTAGTTGAGT TAGATTTATT GACCTACTGT 
CTAAATCGGA GGACAAAAAC TTCTCTTTCC ATTGTAGCTT TGTTGATTCA GCAAAGTAGT 
ACTCACCTGC TACACTTTGA GTTGTGGTGC TTACAGGGCC TAGGGTCCTG CCTTCCAGAA 
AACTTCAGCC TCTTTCTTCC ACTTATCCAT ACCTATTTAC AGTCTTGTAT GCAGGGAGAC 
TTTACACGTC CTTCTAAAGT GTCTAGAAAT GTCATGAATG TGTTGAGAAA GGTCCTGTGG 
AAAGAGCTTC AAAACAGTCT TTTAGGAGAT GATCCTCAGG ATCTTGGAAT TCAAGAAGAG 
GTCCTTTCTA AATTGATTCC CTATTCAAGA GCAAAAGAAC TTAGACTCCT CATTGACAAA 
ATGCCCAGCA TTCTTCAAAA GCCAGAGAAA TCGAAGAGCT GGATTGTATC AGATGCCATA 
GCATTAGTAT TGGAAAAATC TGTGGAAGAG CTGGATGTTT GGAAAAAGAC TTTGTTGGGG 
TCATGTATAA AATGGCTAAT AGCTTCATAC AGCAGTAGTA TCAAAGAAGA AAATACCCAG 
GATGCAGAGA AGTCTATGTT GTTGCGATTT AATGAATTAT TGAACTCAGT TAATGAAGTT 
GATCCTGTTG ATTGGCAGAA GTTTGTGAAG ACAGGCCTTA AGTTCCGCTA CCATGATTAC 
ACATTTTTAA AGACTCTTCA TGCTTGTATA CTGCTGTTAT ATCAGCCTGA AAATTCCCTT 
TGCAAAAACT TAGTGCAGCT GCCACTAATA TATGTGATGA TCACACAACA CTCTATGTTT 
TTGCCAACCA TATTGCAAAC AAAAGAAGAT GACACAAATA TCCAAGTAAA AGAAATGTTA 
GTGGACCTAA TATCAACTAT TGTAAAACTT TGCCCTTCCG TCTGTGAAAG CAACCACTTT 
GCAGTGCTAC TAGGAGGCTA TGGTGCAACC CTCAGTGTTC TAGATCAGAA GATATTGCTG 
CTTCTTAGAT TGTATGAAAG AAATGATCTG AGCCTCATAA ACTTCAGAAT GCTGCTATGG 
GGCCCTGCTG CTGTGGAACA TCACAAGACG TGTAAGAGCC TGGGAAAGTC ACTATGGCAG 
CAGCCAAGTG TGGGAGAAAT CTTATGTCTC CTGGATCGAG AGAGAATGAT GAAGACCATT 
CTTCACTTCC CACAGAATCG AAAACTTCTG CTAAAGGAGG ATGCACAGGA TATATTCAAA 
GACAAAAGCA AAGTAGACTT CGACGAACTT TATGATCCCT GTTTTCTACT TCACCTTTTC 
AGTGAACTTA CCAGACCAGA ATTTATAGTG GATTGTCACA AATTTGTTGA TTCCAATGCC 
CTGGGCCTCA CAGTTTCTGC CCTTAGCAGT TACGATCCTC AGATGCGAGC AGCAGCTTAT 
TATGTTCTGG CTTCCTATTA TTCCCATTTG GAAGGGGCTC GATTTCGAGA ACAGCCACAG 
CTGCTCTATC TCTTGGACAT ATTACAAAAT GGAATCAGAA AACAGAATCT CAGATTGACC 
TATACCTTGA CACTGTTTAT TGCCAAAACT GCCTTGCAGA TTCTGAAACC AGAGGAGCAC 
ATGTACATGA AGATAAACAA ATTCCTTTTG TCACATCAGG ACTTATACTT GAACAAAGTA 
CCGGGATTCT ATAACTTTTT CTACAGCTCT GATTCAGAGT ATAGGATAGA ACAGGAGTGG 
ATATTTGAAC TTTTGAGACA AGGAATTCGA GACACACACT GCTATGATTT GTATTCCTCT 
CAGAGAATTT TCCATATCAT CCTGTCCTTC TTCAACAGCC CATTATGTGA TGAAACAATG 
CAGAACTGGA TTATAGAAAT CTTACAAAAT GCTGCCCAGA TTACCAAAGC TGCCTTTGAA 
ATTGTGCAGG AACATAGCCT TTTAACTTGG ATTTTGCATA TCCTTGGGAA AAAGTTTTTG 
GAAAACCAGA TGCTATCCAA CATTATATGC TTGCTGCATA CTCTCTGGAT ATCCAACTTG 
GGAAACAAAA GAATGGAAAT ACAGGAAGAC CATCCTTGCG AGCTTGTGGC TACACAGTCA 
CCAAAGCTGC TTCCTCTACA ATTTGTCAAT GAATTCCTCT ATGTTTTGGT TGTGTTGGCG 
AAATATCTTC GGCCCACCTT GGACTCTATC CAGATCACTC ACTATTTTAG TACTTTGAGC 
TCTGTGCTGA GGTACCGAGA AACTGTTTTA GAAGCCTTCA AGGAAATGGA CCGATTGACT 
GTAAATGAAA ATGTATTTTC TACCAAAGAT GTCCTCATGC TCTTACATAA GTGGAGCATA 
ATTATGGGAG ATGTTAAACT CCAAGAAGAC TTGAAAACAG TGATTGAAAA ATACCAAGTC 
AAGGAATTAA TGACTTTTCT GCTATATCCT CTACTATCTA AAACCCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023354672.1
CDS	111..6161
Translation	MGNPKRKALKEESKASSAGPAKRPRGEDFTGQQFKTQLKDPQTMVSALETFISIAKMLPQTGLYDVVEGYIKISVECSEIFQLLNGEKRTEHERILIFKTLEAILLRTAGDLSHFHVVGTNIMKKVMTSHMKLIYDSLNASSYKMSQTCLNLMSAMVAQGPDAARDFYSRFDFNKNSLYGLVKIRSPKGLPDVRLAYIQFALSFLIAGDDNTIARVLELKEFLPVIFATGLKEDRISTINLLLSTLKTKVVHNIGVTKTQKVRFFTGYLLNNVASLYRWNGIADVSAENIKPTEEAGKTMVRELVHNFLMDLCCSLKHGINFYDASFGTLGRGGNLTLLNFLLGLKTAADDDLVAELVVNILKVCPDLLTKYFKEVTYSFVPRMKSAWLNNIRLLRKIYEAQPEISPAFKTKEFIPLSRLLSMVMVTTVPNVCNKIMFTQGLKLENKSVNHTILSLISTILKRALKNIDHCLNKDIWQESSIYTPVMMEEFVHLYREAVSKLLPDLNNIVCVWQSLGKQKIDEDGGLEKKNSLEDEKTETTEVDHKHDDAETTLLKAILLQVICLYQMVVPHVVIQCTFDFSKLLKGIISEEGFQEEVPPVLQYYILKVALELPASKFAWLKGQESPNSGERSVFYLLMKMFVTSKHSQLKASTKLLITKILRDTGVFEYTWKELELWVEQLDNTAENRKEVVIQFLECVLLQLVTNPYSYTDKAADFVQEASTLQATVVKQDADNVSIPVSHIDDILDMVDVLMEDTEGLDEEVGFSLNEDMILLTFPFSAIVPTALESRNKLLLGNGNDSGESIVEYLIAVLTDLLHTQRDPLALCLMLQSYDSLQLSNTSLYTRIYQFNRYYNLWIPEQARETLEFQATRPTETSLVVGSAFSTLLTDTYERGVTLLLEENIQGKLREKIAQLPMQKVLLSVKQVMLYLRTTVENFAKLGKSSGPALLQLFMNLLSHLLNHCYHLDTCNQKKCEAAQAESAIFLDEESLATLELANDKTLEDALIVIFRHPTLESWYLALELQTLPSHNLNPILVKLLATHFNAGVLSLLKLSAPILQEMQRMDILSKYLEAITKSVLKELHTLKKTDPPIAYGKPSAQLEALYQLHPYMDGTQLRDITLALLALPKSTLLDQESKKSHQKKKHLSFLGETLKQLITESYQEQSQQGELLSSLEYVKRMSVLLPALVIEELETVFFQALQKEPILVHVVELDLLTYCLNRRTKTSLSIVALLIQQSSTHLLHFELWCLQGLGSCLPENFSLFLPLIHTYLQSCMQGDFTRPSKVSRNVMNVLRKVLWKELQNSLLGDDPQDLGIQEEVLSKLIPYSRAKELRLLIDKMPSILQKPEKSKSWIVSDAIALVLEKSVEELDVWKKTLLGSCIKWLIASYSSSIKEENTQDAEKSMLLRFNELLNSVNEVDPVDWQKFVKTGLKFRYHDYTFLKTLHACILLLYQPENSLCKNLVQLPLIYVMITQHSMFLPTILQTKEDDTNIQVKEMLVDLISTIVKLCPSVCESNHFAVLLGGYGATLSVLDQKILLLLRLYERNDLSLINFRMLLWGPAAVEHHKTCKSLGKSLWQQPSVGEILCLLDRERMMKTILHFPQNRKLLLKEDAQDIFKDKSKVDFDELYDPCFLLHLFSELTRPEFIVDCHKFVDSNALGLTVSALSSYDPQMRAAAYYVLASYYSHLEGARFREQPQLLYLLDILQNGIRKQNLRLTYTLTLFIAKTALQILKPEEHMYMKINKFLLSHQDLYLNKVPGFYNFFYSSDSEYRIEQEWIFELLRQGIRDTHCYDLYSSQRIFHIILSFFNSPLCDETMQNWIIEILQNAAQITKAAFEIVQEHSLLTWILHILGKKFLENQMLSNIICLLHTLWISNLGNKRMEIQEDHPCELVATQSPKLLPLQFVNEFLYVLVVLAKYLRPTLDSIQITHYFSTLSSVLRYRETVLEAFKEMDRLTVNENVFSTKDVLMLLHKWSIIMGDVKLQEDLKTVIEKYQVKELMTFLLYPLLSKTP

Target ORF information:

RefSeq Version	XM_023498904.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii URB1 ribosome biogenesis 1 homolog (S. cerevisiae) (URB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023498904.1

ORF Insert Sequence:

ATGGGAAACC CAAAGAGGAA AGCCCTTAAG GAAGAGAGCA AGGCCTCCTC GGCCGGTCCT 
GCGAAAAGGC CTCGCGGGGA AGATTTCACC GGGCAGCAGT TTAAGACACA GCTCAAAGAC 
CCTCAGACTA TGGTGTCAGC TTTGGAAACT TTTATCTCCA TTGCCAAAAT GTTACCACAA 
ACTGGACTAT ATGATGTCGT GGAAGGATAT ATAAAAATTT CTGTTGAGTG CTCAGAGATT 
TTCCAACTTT TAAATGGAGA AAAAAGAACT GAACATGAAA GGATATTAAT TTTTAAAACT 
TTAGAGGCCA TATTACTGCG AACAGCAGGT GATCTTTCTC ATTTTCATGT TGTGGGAACC 
AACATCATGA AAAAAGTTAT GACTAGCCAT ATGAAGCTGA TTTATGATTC ACTAAATGCT 
TCAAGCTACA AAATGAGTCA AACCTGTTTG AACCTTATGT CCGCTATGGT GGCACAGGGT 
CCAGATGCTG CCAGGGATTT CTATAGCCGA TTTGACTTCA ACAAAAATTC ATTGTATGGT 
TTGGTGAAAA TAAGAAGTCC AAAGGGGCTG CCAGATGTTC GATTGGCATA TATCCAGTTT 
GCCCTTTCCT TCTTAATTGC TGGTGATGAT AATACCATAG CACGAGTGTT GGAGTTGAAA 
GAATTCCTCC CTGTCATTTT TGCGACAGGC TTGAAGGAAG ACAGAATTTC TACTATCAAT 
CTTTTGTTGT CCACCCTGAA AACCAAGGTG GTTCACAATA TTGGTGTTAC AAAGACTCAG 
AAAGTTCGTT TCTTCACTGG ATATTTGTTA AATAATGTGG CTTCTCTCTA TCGTTGGAAT 
GGAATTGCTG ATGTCAGCGC AGAAAATATC AAGCCTACTG AAGAAGCAGG CAAGACCATG 
GTAAGGGAAC TGGTTCACAA CTTCTTAATG GATCTTTGCT GTTCTCTGAA GCATGGCATT 
AACTTCTATG ATGCATCTTT TGGTACTCTG GGCAGAGGAG GGAACTTGAC TCTCTTGAAC 
TTCTTACTGG GTTTGAAGAC AGCAGCAGAT GATGACTTGG TGGCTGAACT TGTAGTGAAC 
ATCCTAAAAG TATGCCCAGA CTTACTTACG AAGTACTTTA AGGAGGTGAC CTATTCCTTT 
GTTCCAAGGA TGAAGTCGGC TTGGTTAAAC AATATCAGGC TACTAAGGAA GATTTATGAG 
GCTCAGCCAG AGATTTCCCC AGCATTTAAA ACTAAAGAGT TTATACCCTT GTCTAGATTG 
CTTTCCATGG TCATGGTGAC AACAGTACCC AACGTGTGCA ACAAAATCAT GTTTACTCAA 
GGATTAAAAT TGGAAAACAA ATCAGTAAAT CATACAATTT TATCACTTAT TTCCACCATT 
TTGAAGAGAG CATTAAAAAA CATTGATCAC TGTCTCAATA AGGATATATG GCAGGAGTCA 
AGCATCTATA CTCCTGTGAT GATGGAGGAA TTTGTGCATC TGTACAGAGA AGCTGTAAGC 
AAGCTTTTGC CAGATTTGAA CAATATTGTA TGTGTGTGGC AATCACTTGG AAAGCAAAAA 
ATAGATGAAG ATGGCGGGTT AGAAAAAAAA AATTCCCTTG AAGATGAGAA AACTGAAACT 
ACAGAAGTAG ACCACAAACA TGATGATGCA GAAACAACTC TTTTGAAGGC TATTTTACTT 
CAGGTCATTT GTCTTTATCA GATGGTTGTT CCCCATGTTG TCATTCAATG TACATTTGAC 
TTCAGCAAGC TTCTAAAAGG TATCATTTCA GAAGAGGGAT TTCAAGAGGA AGTTCCTCCT 
GTCCTTCAGT ACTATATTCT GAAAGTGGCC CTAGAATTGC CTGCCAGCAA ATTTGCCTGG 
TTAAAAGGAC AGGAAAGTCC AAATAGTGGA GAAAGATCTG TGTTTTATTT GCTGATGAAA 
ATGTTTGTGA CAAGTAAACA CTCACAACTT AAAGCATCAA CAAAACTATT GATTACAAAG 
ATTTTACGTG ACACTGGAGT ATTTGAATAT ACATGGAAAG AGCTAGAACT ATGGGTGGAG 
CAATTAGATA ACACAGCAGA AAACAGAAAA GAAGTTGTGA TTCAGTTTTT GGAATGTGTT 
TTGTTGCAAT TGGTAACAAA TCCCTATTCA TATACAGACA AGGCAGCAGA TTTTGTTCAA 
GAAGCTAGTA CACTACAGGC CACTGTGGTG AAGCAAGATG CTGATAATGT TAGCATACCT 
GTATCACACA TTGATGATAT CCTGGATATG GTGGATGTCC TCATGGAGGA CACTGAAGGC 
TTAGATGAGG AAGTTGGTTT CTCTTTAAAT GAAGATATGA TTCTGCTGAC ATTCCCATTC 
AGCGCCATAG TTCCTACAGC TCTGGAGAGC AGGAATAAAT TGCTTCTTGG GAATGGAAAT 
GATTCAGGTG AAAGCATAGT GGAATATCTG ATAGCCGTTC TGACAGACCT TCTACATACA 
CAGCGAGATC CTTTGGCTCT TTGTCTGATG CTTCAGTCTT ATGACAGTTT ACAACTTTCA 
AACACATCTT TGTATACTCG GATTTACCAG TTCAACAGAT ACTATAACCT GTGGATACCT 
GAGCAAGCCA GAGAAACTTT GGAATTTCAA GCTACCAGAC CCACTGAGAC ATCACTTGTG 
GTTGGCTCTG CCTTTTCCAC CTTGTTAACA GATACCTATG AGAGGGGAGT TACCCTCCTT 
TTAGAAGAGA ATATCCAGGG AAAACTGAGA GAAAAAATTG CTCAGCTCCC AATGCAGAAG 
GTTCTGCTCT CTGTGAAGCA GGTGATGCTG TATCTTAGAA CCACTGTGGA GAACTTTGCC 
AAGCTTGGTA AAAGTTCAGG TCCTGCTTTG CTTCAGCTCT TCATGAATTT GCTCAGTCAC 
CTGTTGAACC ATTGTTATCA TTTGGACACA TGTAACCAGA AAAAATGTGA AGCTGCTCAG 
GCCGAATCTG CTATCTTTTT GGATGAGGAG TCCTTGGCCA CACTGGAATT AGCCAATGAT 
AAGACTCTGG AAGATGCGTT GATAGTAATC TTTAGACATC CCACATTGGA GAGCTGGTAT 
TTGGCCCTGG AGCTGCAGAC TCTTCCCTCA CATAACCTCA ATCCAATCCT TGTAAAGCTT 
TTAGCAACTC ATTTCAATGC TGGGGTCCTC TCACTCCTGA AACTGAGTGC CCCAATTCTG 
CAGGAAATGC AACGTATGGA CATCCTTTCC AAGTATTTAG AAGCCATTAC TAAGAGTGTC 
TTGAAAGAAC TACACACCCT GAAGAAGACA GATCCTCCCA TTGCATATGG GAAACCTTCA 
GCTCAGCTGG AAGCTTTGTA CCAACTACAT CCCTACATGG ATGGAACCCA GCTTAGGGAC 
ATTACGCTTG CACTCCTGGC TCTTCCTAAA TCAACCCTTT TGGATCAGGA ATCTAAGAAA 
TCTCACCAAA AAAAAAAACA TCTGAGCTTT TTAGGGGAAA CCCTCAAACA GCTTATCACT 
GAATCCTACC AGGAACAATC CCAGCAAGGT GAATTACTGT CGTCCCTAGA ATATGTGAAG 
AGAATGAGTG TCCTGTTGCC TGCACTGGTC ATTGAAGAGT TAGAGACTGT GTTCTTCCAA 
GCTTTACAGA AAGAGCCAAT TCTGGTCCAT GTAGTTGAGT TAGATTTATT GACCTACTGT 
CTAAATCGGA GGACAAAAAC TTCTCTTTCC ATTGTAGCTT TGTTGATTCA GCAAAGTAGT 
ACTCACCTGC TACACTTTGA GTTGTGGTGC TTACAGGGCC TAGGGTCCTG CCTTCCAGAA 
AACTTCAGCC TCTTTCTTCC ACTTATCCAT ACCTATTTAC AGTCTTGTAT GCAGGGAGAC 
TTTACACGTC CTTCTAAAGT GTCTAGAAAT GTCATGAATG TGTTGAGAAA GGTCCTGTGG 
AAAGAGCTTC AAAACAGTCT TTTAGGAGAT GATCCTCAGG ATCTTGGAAT TCAAGAAGAG 
GTCCTTTCTA AATTGATTCC CTATTCAAGA GCAAAAGAAC TTAGACTCCT CATTGACAAA 
ATGCCCAGCA TTCTTCAAAA GCCAGAGAAA TCGAAGAGCT GGATTGTATC AGATGCCATA 
GCATTAGTAT TGGAAAAATC TGTGGAAGAG CTGGATGTTT GGAAAAAGAC TTTGTTGGGG 
TCATGTATAA AATGGCTAAT AGCTTCATAC AGCAGTAGTA TCAAAGAAGA AAATACCCAG 
GATGCAGAGA AGTCTATGTT GTTGCGATTT AATGAATTAT TGAACTCAGT TAATGAAGTT 
GATCCTGTTG ATTGGCAGAA GTTTGTGAAG ACAGGCCTTA AGTTCCGCTA CCATGATTAC 
ACATTTTTAA AGACTCTTCA TGCTTGTATA CTGCTGTTAT ATCAGCCTGA AAATTCCCTT 
TGCAAAAACT TAGTGCAGCT GCCACTAATA TATGTGATGA TCACACAACA CTCTATGTTT 
TTGCCAACCA TATTGCAAAC AAAAGAAGAT GACACAAATA TCCAAGTAAA AGAAATGTTA 
GTGGACCTAA TATCAACTAT TGTAAAACTT TGCCCTTCCG TCTGTGAAAG CAACCACTTT 
GCAGTGCTAC TAGGAGGCTA TGGTGCAACC CTCAGTGTTC TAGATCAGAA GATATTGCTG 
CTTCTTAGAT TGTATGAAAG AAATGATCTG AGCCTCATAA ACTTCAGAAT GCTGCTATGG 
GGCCCTGCTG CTGTGGAACA TCACAAGACG TGTAAGAGCC TGGGAAAGTC ACTATGGCAG 
CAGCCAAGTG TGGGAGAAAT CTTATGTCTC CTGGATCGAG AGAGAATGAT GAAGACCATT 
CTTCACTTCC CACAGAATCG AAAACTTCTG CTAAAGGAGG ATGCACAGGA TATATTCAAA 
GACAAAAGCA AAGTAGACTT CGACGAACTT TATGATCCCT GTTTTCTACT TCACCTTTTC 
AGTGAACTTA CCAGACCAGA ATTTATAGTG GATTGTCACA AATTTGTTGA TTCCAATGCC 
CTGGGCCTCA CAGTTTCTGC CCTTAGCAGT TACGATCCTC AGATGCGAGC AGCAGCTTAT 
TATGTTCTGG CTTCCTATTA TTCCCATTTG GAAGGGGCTC GATTTCGAGA ACAGCCACAG 
CTGCTCTATC TCTTGGACAT ATTACAAAAT GGAATCAGAA AACAGAATCT CAGATTGACC 
TATACCTTGA CACTGTTTAT TGCCAAAACT GCCTTGCAGA TTCTGAAACC AGAGGAGCAC 
ATGTACATGA AGATAAACAA ATTCCTTTTG TCACATCAGG ACTTATACTT GAACAAAGTA 
CCGGGATTCT ATAACTTTTT CTACAGCTCT GATTCAGAGT ATAGGATAGA ACAGGAGTGG 
ATATTTGAAC TTTTGAGACA AGGAATTCGA GACACACACT GCTATGATTT GTATTCCTCT 
CAGAGAATTT TCCATATCAT CCTGTCCTTC TTCAACAGCC CATTATGTGA TGAAACAATG 
CAGAACTGGA TTATAGAAAT CTTACAAAAT GCTGCCCAGA TTACCAAAGC TGCCTTTGAA 
ATTGTGCAGG AACATAGCCT TTTAACTTGG ATTTTGCATA TCCTTGGGAA AAAGTTTTTG 
GAAAACCAGA TGCTATCCAA CATTATATGC TTGCTGCATA CTCTCTGGAT ATCCAACTTG 
GGAAACAAAA GAATGGAAAT ACAGGAAGAC CATCCTTGCG AGCTTGTGGC TACACAGTCA 
CCAAAGCTGC TTCCTCTACA ATTTGTCAAT GAATTCCTCT ATGTTTTGGT TGTGTTGGCG 
AAATATCTTC GGCCCACCTT GGACTCTATC CAGATCACTC ACTATTTTAG TACTTTGAGC 
TCTGTGCTGA GGTACCGAGA AACTGTTTTA GAAGCCTTCA AGGAAATGGA CCGATTGACT 
GTAAATGAAA ATGTATTTTC TACCAAAGAT GTCCTCATGC TCTTACATAA GTGGAGCATA 
ATTATGGGAG ATGTTAAACT CCAAGAAGAC TTGAAAACAG TGATTGAAAA ATACCAAGTC 
AAGGAATTAA TGACTTTTCT GCTATATCCT CTACTATCTA AAACCCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

URB1 ( XM_023498904.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023354672.1 Sarcophilus harrisii URB1 ribosome biogenesis homolog (URB1), transcript variant X2, mRNA.

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CloneID	OSa151555
Clone ID Related Accession (Same CDS sequence)	XM_023498904.1 , XM_023498904.2
Accession Version	XM_023498904.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	nucleolar pre-ribosomal-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_023498904.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACC CAAAGAGGAA AGCCCTTAAG GAAGAGAGCA AGGCCTCCTC GGCCGGTCCT 
GCGAAAAGGC CTCGCGGGGA AGATTTCACC GGGCAGCAGT TTAAGACACA GCTCAAAGAC 
CCTCAGACTA TGGTGTCAGC TTTGGAAACT TTTATCTCCA TTGCCAAAAT GTTACCACAA 
ACTGGACTAT ATGATGTCGT GGAAGGATAT ATAAAAATTT CTGTTGAGTG CTCAGAGATT 
TTCCAACTTT TAAATGGAGA AAAAAGAACT GAACATGAAA GGATATTAAT TTTTAAAACT 
TTAGAGGCCA TATTACTGCG AACAGCAGGT GATCTTTCTC ATTTTCATGT TGTGGGAACC 
AACATCATGA AAAAAGTTAT GACTAGCCAT ATGAAGCTGA TTTATGATTC ACTAAATGCT 
TCAAGCTACA AAATGAGTCA AACCTGTTTG AACCTTATGT CCGCTATGGT GGCACAGGGT 
CCAGATGCTG CCAGGGATTT CTATAGCCGA TTTGACTTCA ACAAAAATTC ATTGTATGGT 
TTGGTGAAAA TAAGAAGTCC AAAGGGGCTG CCAGATGTTC GATTGGCATA TATCCAGTTT 
GCCCTTTCCT TCTTAATTGC TGGTGATGAT AATACCATAG CACGAGTGTT GGAGTTGAAA 
GAATTCCTCC CTGTCATTTT TGCGACAGGC TTGAAGGAAG ACAGAATTTC TACTATCAAT 
CTTTTGTTGT CCACCCTGAA AACCAAGGTG GTTCACAATA TTGGTGTTAC AAAGACTCAG 
AAAGTTCGTT TCTTCACTGG ATATTTGTTA AATAATGTGG CTTCTCTCTA TCGTTGGAAT 
GGAATTGCTG ATGTCAGCGC AGAAAATATC AAGCCTACTG AAGAAGCAGG CAAGACCATG 
GTAAGGGAAC TGGTTCACAA CTTCTTAATG GATCTTTGCT GTTCTCTGAA GCATGGCATT 
AACTTCTATG ATGCATCTTT TGGTACTCTG GGCAGAGGAG GGAACTTGAC TCTCTTGAAC 
TTCTTACTGG GTTTGAAGAC AGCAGCAGAT GATGACTTGG TGGCTGAACT TGTAGTGAAC 
ATCCTAAAAG TATGCCCAGA CTTACTTACG AAGTACTTTA AGGAGGTGAC CTATTCCTTT 
GTTCCAAGGA TGAAGTCGGC TTGGTTAAAC AATATCAGGC TACTAAGGAA GATTTATGAG 
GCTCAGCCAG AGATTTCCCC AGCATTTAAA ACTAAAGAGT TTATACCCTT GTCTAGATTG 
CTTTCCATGG TCATGGTGAC AACAGTACCC AACGTGTGCA ACAAAATCAT GTTTACTCAA 
GGATTAAAAT TGGAAAACAA ATCAGTAAAT CATACAATTT TATCACTTAT TTCCACCATT 
TTGAAGAGAG CATTAAAAAA CATTGATCAC TGTCTCAATA AGGATATATG GCAGGAGTCA 
AGCATCTATA CTCCTGTGAT GATGGAGGAA TTTGTGCATC TGTACAGAGA AGCTGTAAGC 
AAGCTTTTGC CAGATTTGAA CAATATTGTA TGTGTGTGGC AATCACTTGG AAAGCAAAAA 
ATAGATGAAG ATGGCGGGTT AGAAAAAAAA AATTCCCTTG AAGATGAGAA AACTGAAACT 
ACAGAAGTAG ACCACAAACA TGATGATGCA GAAACAACTC TTTTGAAGGC TATTTTACTT 
CAGGTCATTT GTCTTTATCA GATGGTTGTT CCCCATGTTG TCATTCAATG TACATTTGAC 
TTCAGCAAGC TTCTAAAAGG TATCATTTCA GAAGAGGGAT TTCAAGAGGA AGTTCCTCCT 
GTCCTTCAGT ACTATATTCT GAAAGTGGCC CTAGAATTGC CTGCCAGCAA ATTTGCCTGG 
TTAAAAGGAC AGGAAAGTCC AAATAGTGGA GAAAGATCTG TGTTTTATTT GCTGATGAAA 
ATGTTTGTGA CAAGTAAACA CTCACAACTT AAAGCATCAA CAAAACTATT GATTACAAAG 
ATTTTACGTG ACACTGGAGT ATTTGAATAT ACATGGAAAG AGCTAGAACT ATGGGTGGAG 
CAATTAGATA ACACAGCAGA AAACAGAAAA GAAGTTGTGA TTCAGTTTTT GGAATGTGTT 
TTGTTGCAAT TGGTAACAAA TCCCTATTCA TATACAGACA AGGCAGCAGA TTTTGTTCAA 
GAAGCTAGTA CACTACAGGC CACTGTGGTG AAGCAAGATG CTGATAATGT TAGCATACCT 
GTATCACACA TTGATGATAT CCTGGATATG GTGGATGTCC TCATGGAGGA CACTGAAGGC 
TTAGATGAGG AAGTTGGTTT CTCTTTAAAT GAAGATATGA TTCTGCTGAC ATTCCCATTC 
AGCGCCATAG TTCCTACAGC TCTGGAGAGC AGGAATAAAT TGCTTCTTGG GAATGGAAAT 
GATTCAGGTG AAAGCATAGT GGAATATCTG ATAGCCGTTC TGACAGACCT TCTACATACA 
CAGCGAGATC CTTTGGCTCT TTGTCTGATG CTTCAGTCTT ATGACAGTTT ACAACTTTCA 
AACACATCTT TGTATACTCG GATTTACCAG TTCAACAGAT ACTATAACCT GTGGATACCT 
GAGCAAGCCA GAGAAACTTT GGAATTTCAA GCTACCAGAC CCACTGAGAC ATCACTTGTG 
GTTGGCTCTG CCTTTTCCAC CTTGTTAACA GATACCTATG AGAGGGGAGT TACCCTCCTT 
TTAGAAGAGA ATATCCAGGG AAAACTGAGA GAAAAAATTG CTCAGCTCCC AATGCAGAAG 
GTTCTGCTCT CTGTGAAGCA GGTGATGCTG TATCTTAGAA CCACTGTGGA GAACTTTGCC 
AAGCTTGGTA AAAGTTCAGG TCCTGCTTTG CTTCAGCTCT TCATGAATTT GCTCAGTCAC 
CTGTTGAACC ATTGTTATCA TTTGGACACA TGTAACCAGA AAAAATGTGA AGCTGCTCAG 
GCCGAATCTG CTATCTTTTT GGATGAGGAG TCCTTGGCCA CACTGGAATT AGCCAATGAT 
AAGACTCTGG AAGATGCGTT GATAGTAATC TTTAGACATC CCACATTGGA GAGCTGGTAT 
TTGGCCCTGG AGCTGCAGAC TCTTCCCTCA CATAACCTCA ATCCAATCCT TGTAAAGCTT 
TTAGCAACTC ATTTCAATGC TGGGGTCCTC TCACTCCTGA AACTGAGTGC CCCAATTCTG 
CAGGAAATGC AACGTATGGA CATCCTTTCC AAGTATTTAG AAGCCATTAC TAAGAGTGTC 
TTGAAAGAAC TACACACCCT GAAGAAGACA GATCCTCCCA TTGCATATGG GAAACCTTCA 
GCTCAGCTGG AAGCTTTGTA CCAACTACAT CCCTACATGG ATGGAACCCA GCTTAGGGAC 
ATTACGCTTG CACTCCTGGC TCTTCCTAAA TCAACCCTTT TGGATCAGGA ATCTAAGAAA 
TCTCACCAAA AAAAAAAACA TCTGAGCTTT TTAGGGGAAA CCCTCAAACA GCTTATCACT 
GAATCCTACC AGGAACAATC CCAGCAAGGT GAATTACTGT CGTCCCTAGA ATATGTGAAG 
AGAATGAGTG TCCTGTTGCC TGCACTGGTC ATTGAAGAGT TAGAGACTGT GTTCTTCCAA 
GCTTTACAGA AAGAGCCAAT TCTGGTCCAT GTAGTTGAGT TAGATTTATT GACCTACTGT 
CTAAATCGGA GGACAAAAAC TTCTCTTTCC ATTGTAGCTT TGTTGATTCA GCAAAGTAGT 
ACTCACCTGC TACACTTTGA GTTGTGGTGC TTACAGGGCC TAGGGTCCTG CCTTCCAGAA 
AACTTCAGCC TCTTTCTTCC ACTTATCCAT ACCTATTTAC AGTCTTGTAT GCAGGGAGAC 
TTTACACGTC CTTCTAAAGT GTCTAGAAAT GTCATGAATG TGTTGAGAAA GGTCCTGTGG 
AAAGAGCTTC AAAACAGTCT TTTAGGAGAT GATCCTCAGG ATCTTGGAAT TCAAGAAGAG 
GTCCTTTCTA AATTGATTCC CTATTCAAGA GCAAAAGAAC TTAGACTCCT CATTGACAAA 
ATGCCCAGCA TTCTTCAAAA GCCAGAGAAA TCGAAGAGCT GGATTGTATC AGATGCCATA 
GCATTAGTAT TGGAAAAATC TGTGGAAGAG CTGGATGTTT GGAAAAAGAC TTTGTTGGGG 
TCATGTATAA AATGGCTAAT AGCTTCATAC AGCAGTAGTA TCAAAGAAGA AAATACCCAG 
GATGCAGAGA AGTCTATGTT GTTGCGATTT AATGAATTAT TGAACTCAGT TAATGAAGTT 
GATCCTGTTG ATTGGCAGAA GTTTGTGAAG ACAGGCCTTA AGTTCCGCTA CCATGATTAC 
ACATTTTTAA AGACTCTTCA TGCTTGTATA CTGCTGTTAT ATCAGCCTGA AAATTCCCTT 
TGCAAAAACT TAGTGCAGCT GCCACTAATA TATGTGATGA TCACACAACA CTCTATGTTT 
TTGCCAACCA TATTGCAAAC AAAAGAAGAT GACACAAATA TCCAAGTAAA AGAAATGTTA 
GTGGACCTAA TATCAACTAT TGTAAAACTT TGCCCTTCCG TCTGTGAAAG CAACCACTTT 
GCAGTGCTAC TAGGAGGCTA TGGTGCAACC CTCAGTGTTC TAGATCAGAA GATATTGCTG 
CTTCTTAGAT TGTATGAAAG AAATGATCTG AGCCTCATAA ACTTCAGAAT GCTGCTATGG 
GGCCCTGCTG CTGTGGAACA TCACAAGACG TGTAAGAGCC TGGGAAAGTC ACTATGGCAG 
CAGCCAAGTG TGGGAGAAAT CTTATGTCTC CTGGATCGAG AGAGAATGAT GAAGACCATT 
CTTCACTTCC CACAGAATCG AAAACTTCTG CTAAAGGAGG ATGCACAGGA TATATTCAAA 
GACAAAAGCA AAGTAGACTT CGACGAACTT TATGATCCCT GTTTTCTACT TCACCTTTTC 
AGTGAACTTA CCAGACCAGA ATTTATAGTG GATTGTCACA AATTTGTTGA TTCCAATGCC 
CTGGGCCTCA CAGTTTCTGC CCTTAGCAGT TACGATCCTC AGATGCGAGC AGCAGCTTAT 
TATGTTCTGG CTTCCTATTA TTCCCATTTG GAAGGGGCTC GATTTCGAGA ACAGCCACAG 
CTGCTCTATC TCTTGGACAT ATTACAAAAT GGAATCAGAA AACAGAATCT CAGATTGACC 
TATACCTTGA CACTGTTTAT TGCCAAAACT GCCTTGCAGA TTCTGAAACC AGAGGAGCAC 
ATGTACATGA AGATAAACAA ATTCCTTTTG TCACATCAGG ACTTATACTT GAACAAAGTA 
CCGGGATTCT ATAACTTTTT CTACAGCTCT GATTCAGAGT ATAGGATAGA ACAGGAGTGG 
ATATTTGAAC TTTTGAGACA AGGAATTCGA GACACACACT GCTATGATTT GTATTCCTCT 
CAGAGAATTT TCCATATCAT CCTGTCCTTC TTCAACAGCC CATTATGTGA TGAAACAATG 
CAGAACTGGA TTATAGAAAT CTTACAAAAT GCTGCCCAGA TTACCAAAGC TGCCTTTGAA 
ATTGTGCAGG AACATAGCCT TTTAACTTGG ATTTTGCATA TCCTTGGGAA AAAGTTTTTG 
GAAAACCAGA TGCTATCCAA CATTATATGC TTGCTGCATA CTCTCTGGAT ATCCAACTTG 
GGAAACAAAA GAATGGAAAT ACAGGAAGAC CATCCTTGCG AGCTTGTGGC TACACAGTCA 
CCAAAGCTGC TTCCTCTACA ATTTGTCAAT GAATTCCTCT ATGTTTTGGT TGTGTTGGCG 
AAATATCTTC GGCCCACCTT GGACTCTATC CAGATCACTC ACTATTTTAG TACTTTGAGC 
TCTGTGCTGA GGTACCGAGA AACTGTTTTA GAAGCCTTCA AGGAAATGGA CCGATTGACT 
GTAAATGAAA ATGTATTTTC TACCAAAGAT GTCCTCATGC TCTTACATAA GTGGAGCATA 
ATTATGGGAG ATGTTAAACT CCAAGAAGAC TTGAAAACAG TGATTGAAAA ATACCAAGTC 
AAGGAATTAA TGACTTTTCT GCTATATCCT CTACTATCTA AAACCCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023354672.1
CDS	208..6258
Translation	MGNPKRKALKEESKASSAGPAKRPRGEDFTGQQFKTQLKDPQTMVSALETFISIAKMLPQTGLYDVVEGYIKISVECSEIFQLLNGEKRTEHERILIFKTLEAILLRTAGDLSHFHVVGTNIMKKVMTSHMKLIYDSLNASSYKMSQTCLNLMSAMVAQGPDAARDFYSRFDFNKNSLYGLVKIRSPKGLPDVRLAYIQFALSFLIAGDDNTIARVLELKEFLPVIFATGLKEDRISTINLLLSTLKTKVVHNIGVTKTQKVRFFTGYLLNNVASLYRWNGIADVSAENIKPTEEAGKTMVRELVHNFLMDLCCSLKHGINFYDASFGTLGRGGNLTLLNFLLGLKTAADDDLVAELVVNILKVCPDLLTKYFKEVTYSFVPRMKSAWLNNIRLLRKIYEAQPEISPAFKTKEFIPLSRLLSMVMVTTVPNVCNKIMFTQGLKLENKSVNHTILSLISTILKRALKNIDHCLNKDIWQESSIYTPVMMEEFVHLYREAVSKLLPDLNNIVCVWQSLGKQKIDEDGGLEKKNSLEDEKTETTEVDHKHDDAETTLLKAILLQVICLYQMVVPHVVIQCTFDFSKLLKGIISEEGFQEEVPPVLQYYILKVALELPASKFAWLKGQESPNSGERSVFYLLMKMFVTSKHSQLKASTKLLITKILRDTGVFEYTWKELELWVEQLDNTAENRKEVVIQFLECVLLQLVTNPYSYTDKAADFVQEASTLQATVVKQDADNVSIPVSHIDDILDMVDVLMEDTEGLDEEVGFSLNEDMILLTFPFSAIVPTALESRNKLLLGNGNDSGESIVEYLIAVLTDLLHTQRDPLALCLMLQSYDSLQLSNTSLYTRIYQFNRYYNLWIPEQARETLEFQATRPTETSLVVGSAFSTLLTDTYERGVTLLLEENIQGKLREKIAQLPMQKVLLSVKQVMLYLRTTVENFAKLGKSSGPALLQLFMNLLSHLLNHCYHLDTCNQKKCEAAQAESAIFLDEESLATLELANDKTLEDALIVIFRHPTLESWYLALELQTLPSHNLNPILVKLLATHFNAGVLSLLKLSAPILQEMQRMDILSKYLEAITKSVLKELHTLKKTDPPIAYGKPSAQLEALYQLHPYMDGTQLRDITLALLALPKSTLLDQESKKSHQKKKHLSFLGETLKQLITESYQEQSQQGELLSSLEYVKRMSVLLPALVIEELETVFFQALQKEPILVHVVELDLLTYCLNRRTKTSLSIVALLIQQSSTHLLHFELWCLQGLGSCLPENFSLFLPLIHTYLQSCMQGDFTRPSKVSRNVMNVLRKVLWKELQNSLLGDDPQDLGIQEEVLSKLIPYSRAKELRLLIDKMPSILQKPEKSKSWIVSDAIALVLEKSVEELDVWKKTLLGSCIKWLIASYSSSIKEENTQDAEKSMLLRFNELLNSVNEVDPVDWQKFVKTGLKFRYHDYTFLKTLHACILLLYQPENSLCKNLVQLPLIYVMITQHSMFLPTILQTKEDDTNIQVKEMLVDLISTIVKLCPSVCESNHFAVLLGGYGATLSVLDQKILLLLRLYERNDLSLINFRMLLWGPAAVEHHKTCKSLGKSLWQQPSVGEILCLLDRERMMKTILHFPQNRKLLLKEDAQDIFKDKSKVDFDELYDPCFLLHLFSELTRPEFIVDCHKFVDSNALGLTVSALSSYDPQMRAAAYYVLASYYSHLEGARFREQPQLLYLLDILQNGIRKQNLRLTYTLTLFIAKTALQILKPEEHMYMKINKFLLSHQDLYLNKVPGFYNFFYSSDSEYRIEQEWIFELLRQGIRDTHCYDLYSSQRIFHIILSFFNSPLCDETMQNWIIEILQNAAQITKAAFEIVQEHSLLTWILHILGKKFLENQMLSNIICLLHTLWISNLGNKRMEIQEDHPCELVATQSPKLLPLQFVNEFLYVLVVLAKYLRPTLDSIQITHYFSTLSSVLRYRETVLEAFKEMDRLTVNENVFSTKDVLMLLHKWSIIMGDVKLQEDLKTVIEKYQVKELMTFLLYPLLSKTP

Target ORF information:

RefSeq Version	XM_023498904.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii URB1 ribosome biogenesis homolog (URB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023498904.2

ORF Insert Sequence:

ATGGGAAACC CAAAGAGGAA AGCCCTTAAG GAAGAGAGCA AGGCCTCCTC GGCCGGTCCT 
GCGAAAAGGC CTCGCGGGGA AGATTTCACC GGGCAGCAGT TTAAGACACA GCTCAAAGAC 
CCTCAGACTA TGGTGTCAGC TTTGGAAACT TTTATCTCCA TTGCCAAAAT GTTACCACAA 
ACTGGACTAT ATGATGTCGT GGAAGGATAT ATAAAAATTT CTGTTGAGTG CTCAGAGATT 
TTCCAACTTT TAAATGGAGA AAAAAGAACT GAACATGAAA GGATATTAAT TTTTAAAACT 
TTAGAGGCCA TATTACTGCG AACAGCAGGT GATCTTTCTC ATTTTCATGT TGTGGGAACC 
AACATCATGA AAAAAGTTAT GACTAGCCAT ATGAAGCTGA TTTATGATTC ACTAAATGCT 
TCAAGCTACA AAATGAGTCA AACCTGTTTG AACCTTATGT CCGCTATGGT GGCACAGGGT 
CCAGATGCTG CCAGGGATTT CTATAGCCGA TTTGACTTCA ACAAAAATTC ATTGTATGGT 
TTGGTGAAAA TAAGAAGTCC AAAGGGGCTG CCAGATGTTC GATTGGCATA TATCCAGTTT 
GCCCTTTCCT TCTTAATTGC TGGTGATGAT AATACCATAG CACGAGTGTT GGAGTTGAAA 
GAATTCCTCC CTGTCATTTT TGCGACAGGC TTGAAGGAAG ACAGAATTTC TACTATCAAT 
CTTTTGTTGT CCACCCTGAA AACCAAGGTG GTTCACAATA TTGGTGTTAC AAAGACTCAG 
AAAGTTCGTT TCTTCACTGG ATATTTGTTA AATAATGTGG CTTCTCTCTA TCGTTGGAAT 
GGAATTGCTG ATGTCAGCGC AGAAAATATC AAGCCTACTG AAGAAGCAGG CAAGACCATG 
GTAAGGGAAC TGGTTCACAA CTTCTTAATG GATCTTTGCT GTTCTCTGAA GCATGGCATT 
AACTTCTATG ATGCATCTTT TGGTACTCTG GGCAGAGGAG GGAACTTGAC TCTCTTGAAC 
TTCTTACTGG GTTTGAAGAC AGCAGCAGAT GATGACTTGG TGGCTGAACT TGTAGTGAAC 
ATCCTAAAAG TATGCCCAGA CTTACTTACG AAGTACTTTA AGGAGGTGAC CTATTCCTTT 
GTTCCAAGGA TGAAGTCGGC TTGGTTAAAC AATATCAGGC TACTAAGGAA GATTTATGAG 
GCTCAGCCAG AGATTTCCCC AGCATTTAAA ACTAAAGAGT TTATACCCTT GTCTAGATTG 
CTTTCCATGG TCATGGTGAC AACAGTACCC AACGTGTGCA ACAAAATCAT GTTTACTCAA 
GGATTAAAAT TGGAAAACAA ATCAGTAAAT CATACAATTT TATCACTTAT TTCCACCATT 
TTGAAGAGAG CATTAAAAAA CATTGATCAC TGTCTCAATA AGGATATATG GCAGGAGTCA 
AGCATCTATA CTCCTGTGAT GATGGAGGAA TTTGTGCATC TGTACAGAGA AGCTGTAAGC 
AAGCTTTTGC CAGATTTGAA CAATATTGTA TGTGTGTGGC AATCACTTGG AAAGCAAAAA 
ATAGATGAAG ATGGCGGGTT AGAAAAAAAA AATTCCCTTG AAGATGAGAA AACTGAAACT 
ACAGAAGTAG ACCACAAACA TGATGATGCA GAAACAACTC TTTTGAAGGC TATTTTACTT 
CAGGTCATTT GTCTTTATCA GATGGTTGTT CCCCATGTTG TCATTCAATG TACATTTGAC 
TTCAGCAAGC TTCTAAAAGG TATCATTTCA GAAGAGGGAT TTCAAGAGGA AGTTCCTCCT 
GTCCTTCAGT ACTATATTCT GAAAGTGGCC CTAGAATTGC CTGCCAGCAA ATTTGCCTGG 
TTAAAAGGAC AGGAAAGTCC AAATAGTGGA GAAAGATCTG TGTTTTATTT GCTGATGAAA 
ATGTTTGTGA CAAGTAAACA CTCACAACTT AAAGCATCAA CAAAACTATT GATTACAAAG 
ATTTTACGTG ACACTGGAGT ATTTGAATAT ACATGGAAAG AGCTAGAACT ATGGGTGGAG 
CAATTAGATA ACACAGCAGA AAACAGAAAA GAAGTTGTGA TTCAGTTTTT GGAATGTGTT 
TTGTTGCAAT TGGTAACAAA TCCCTATTCA TATACAGACA AGGCAGCAGA TTTTGTTCAA 
GAAGCTAGTA CACTACAGGC CACTGTGGTG AAGCAAGATG CTGATAATGT TAGCATACCT 
GTATCACACA TTGATGATAT CCTGGATATG GTGGATGTCC TCATGGAGGA CACTGAAGGC 
TTAGATGAGG AAGTTGGTTT CTCTTTAAAT GAAGATATGA TTCTGCTGAC ATTCCCATTC 
AGCGCCATAG TTCCTACAGC TCTGGAGAGC AGGAATAAAT TGCTTCTTGG GAATGGAAAT 
GATTCAGGTG AAAGCATAGT GGAATATCTG ATAGCCGTTC TGACAGACCT TCTACATACA 
CAGCGAGATC CTTTGGCTCT TTGTCTGATG CTTCAGTCTT ATGACAGTTT ACAACTTTCA 
AACACATCTT TGTATACTCG GATTTACCAG TTCAACAGAT ACTATAACCT GTGGATACCT 
GAGCAAGCCA GAGAAACTTT GGAATTTCAA GCTACCAGAC CCACTGAGAC ATCACTTGTG 
GTTGGCTCTG CCTTTTCCAC CTTGTTAACA GATACCTATG AGAGGGGAGT TACCCTCCTT 
TTAGAAGAGA ATATCCAGGG AAAACTGAGA GAAAAAATTG CTCAGCTCCC AATGCAGAAG 
GTTCTGCTCT CTGTGAAGCA GGTGATGCTG TATCTTAGAA CCACTGTGGA GAACTTTGCC 
AAGCTTGGTA AAAGTTCAGG TCCTGCTTTG CTTCAGCTCT TCATGAATTT GCTCAGTCAC 
CTGTTGAACC ATTGTTATCA TTTGGACACA TGTAACCAGA AAAAATGTGA AGCTGCTCAG 
GCCGAATCTG CTATCTTTTT GGATGAGGAG TCCTTGGCCA CACTGGAATT AGCCAATGAT 
AAGACTCTGG AAGATGCGTT GATAGTAATC TTTAGACATC CCACATTGGA GAGCTGGTAT 
TTGGCCCTGG AGCTGCAGAC TCTTCCCTCA CATAACCTCA ATCCAATCCT TGTAAAGCTT 
TTAGCAACTC ATTTCAATGC TGGGGTCCTC TCACTCCTGA AACTGAGTGC CCCAATTCTG 
CAGGAAATGC AACGTATGGA CATCCTTTCC AAGTATTTAG AAGCCATTAC TAAGAGTGTC 
TTGAAAGAAC TACACACCCT GAAGAAGACA GATCCTCCCA TTGCATATGG GAAACCTTCA 
GCTCAGCTGG AAGCTTTGTA CCAACTACAT CCCTACATGG ATGGAACCCA GCTTAGGGAC 
ATTACGCTTG CACTCCTGGC TCTTCCTAAA TCAACCCTTT TGGATCAGGA ATCTAAGAAA 
TCTCACCAAA AAAAAAAACA TCTGAGCTTT TTAGGGGAAA CCCTCAAACA GCTTATCACT 
GAATCCTACC AGGAACAATC CCAGCAAGGT GAATTACTGT CGTCCCTAGA ATATGTGAAG 
AGAATGAGTG TCCTGTTGCC TGCACTGGTC ATTGAAGAGT TAGAGACTGT GTTCTTCCAA 
GCTTTACAGA AAGAGCCAAT TCTGGTCCAT GTAGTTGAGT TAGATTTATT GACCTACTGT 
CTAAATCGGA GGACAAAAAC TTCTCTTTCC ATTGTAGCTT TGTTGATTCA GCAAAGTAGT 
ACTCACCTGC TACACTTTGA GTTGTGGTGC TTACAGGGCC TAGGGTCCTG CCTTCCAGAA 
AACTTCAGCC TCTTTCTTCC ACTTATCCAT ACCTATTTAC AGTCTTGTAT GCAGGGAGAC 
TTTACACGTC CTTCTAAAGT GTCTAGAAAT GTCATGAATG TGTTGAGAAA GGTCCTGTGG 
AAAGAGCTTC AAAACAGTCT TTTAGGAGAT GATCCTCAGG ATCTTGGAAT TCAAGAAGAG 
GTCCTTTCTA AATTGATTCC CTATTCAAGA GCAAAAGAAC TTAGACTCCT CATTGACAAA 
ATGCCCAGCA TTCTTCAAAA GCCAGAGAAA TCGAAGAGCT GGATTGTATC AGATGCCATA 
GCATTAGTAT TGGAAAAATC TGTGGAAGAG CTGGATGTTT GGAAAAAGAC TTTGTTGGGG 
TCATGTATAA AATGGCTAAT AGCTTCATAC AGCAGTAGTA TCAAAGAAGA AAATACCCAG 
GATGCAGAGA AGTCTATGTT GTTGCGATTT AATGAATTAT TGAACTCAGT TAATGAAGTT 
GATCCTGTTG ATTGGCAGAA GTTTGTGAAG ACAGGCCTTA AGTTCCGCTA CCATGATTAC 
ACATTTTTAA AGACTCTTCA TGCTTGTATA CTGCTGTTAT ATCAGCCTGA AAATTCCCTT 
TGCAAAAACT TAGTGCAGCT GCCACTAATA TATGTGATGA TCACACAACA CTCTATGTTT 
TTGCCAACCA TATTGCAAAC AAAAGAAGAT GACACAAATA TCCAAGTAAA AGAAATGTTA 
GTGGACCTAA TATCAACTAT TGTAAAACTT TGCCCTTCCG TCTGTGAAAG CAACCACTTT 
GCAGTGCTAC TAGGAGGCTA TGGTGCAACC CTCAGTGTTC TAGATCAGAA GATATTGCTG 
CTTCTTAGAT TGTATGAAAG AAATGATCTG AGCCTCATAA ACTTCAGAAT GCTGCTATGG 
GGCCCTGCTG CTGTGGAACA TCACAAGACG TGTAAGAGCC TGGGAAAGTC ACTATGGCAG 
CAGCCAAGTG TGGGAGAAAT CTTATGTCTC CTGGATCGAG AGAGAATGAT GAAGACCATT 
CTTCACTTCC CACAGAATCG AAAACTTCTG CTAAAGGAGG ATGCACAGGA TATATTCAAA 
GACAAAAGCA AAGTAGACTT CGACGAACTT TATGATCCCT GTTTTCTACT TCACCTTTTC 
AGTGAACTTA CCAGACCAGA ATTTATAGTG GATTGTCACA AATTTGTTGA TTCCAATGCC 
CTGGGCCTCA CAGTTTCTGC CCTTAGCAGT TACGATCCTC AGATGCGAGC AGCAGCTTAT 
TATGTTCTGG CTTCCTATTA TTCCCATTTG GAAGGGGCTC GATTTCGAGA ACAGCCACAG 
CTGCTCTATC TCTTGGACAT ATTACAAAAT GGAATCAGAA AACAGAATCT CAGATTGACC 
TATACCTTGA CACTGTTTAT TGCCAAAACT GCCTTGCAGA TTCTGAAACC AGAGGAGCAC 
ATGTACATGA AGATAAACAA ATTCCTTTTG TCACATCAGG ACTTATACTT GAACAAAGTA 
CCGGGATTCT ATAACTTTTT CTACAGCTCT GATTCAGAGT ATAGGATAGA ACAGGAGTGG 
ATATTTGAAC TTTTGAGACA AGGAATTCGA GACACACACT GCTATGATTT GTATTCCTCT 
CAGAGAATTT TCCATATCAT CCTGTCCTTC TTCAACAGCC CATTATGTGA TGAAACAATG 
CAGAACTGGA TTATAGAAAT CTTACAAAAT GCTGCCCAGA TTACCAAAGC TGCCTTTGAA 
ATTGTGCAGG AACATAGCCT TTTAACTTGG ATTTTGCATA TCCTTGGGAA AAAGTTTTTG 
GAAAACCAGA TGCTATCCAA CATTATATGC TTGCTGCATA CTCTCTGGAT ATCCAACTTG 
GGAAACAAAA GAATGGAAAT ACAGGAAGAC CATCCTTGCG AGCTTGTGGC TACACAGTCA 
CCAAAGCTGC TTCCTCTACA ATTTGTCAAT GAATTCCTCT ATGTTTTGGT TGTGTTGGCG 
AAATATCTTC GGCCCACCTT GGACTCTATC CAGATCACTC ACTATTTTAG TACTTTGAGC 
TCTGTGCTGA GGTACCGAGA AACTGTTTTA GAAGCCTTCA AGGAAATGGA CCGATTGACT 
GTAAATGAAA ATGTATTTTC TACCAAAGAT GTCCTCATGC TCTTACATAA GTGGAGCATA 
ATTATGGGAG ATGTTAAACT CCAAGAAGAC TTGAAAACAG TGATTGAAAA ATACCAAGTC 
AAGGAATTAA TGACTTTTCT GCTATATCCT CTACTATCTA AAACCCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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URB1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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