PRPF8 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRPF8
Entrez Gene ID	100928308
Full Name	pre-mRNA processing factor 8
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012549867.1	XP_012405321.1	pre-mRNA-processing-splicing factor 8
XM_023503711.1	XP_023359479.1	pre-mRNA-processing-splicing factor 8
XM_003770117.1	XP_003770165.1	pre-mRNA-processing-splicing factor 8
XM_031967785.1	XP_031823645.1	pre-mRNA-processing-splicing factor 8
XM_023503711.2	XP_023359479.2	pre-mRNA-processing-splicing factor 8
XM_031967786.1	XP_031823646.1	pre-mRNA-processing-splicing factor 8

Pathways from BioSystems
Gene Ontology

The following PRPF8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRPF8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa132365	XM_023503711.1 Latest version!	Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa132365	XM_012549867.1 Latest version!	Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa132365	XM_003770117.1 Latest version!	Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa234804	XM_023503711.2 Latest version!	Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa234804	XM_031967785.1 Latest version!	Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa234804	XM_031967786.1 Latest version!	Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRPF8 ( XM_012549867.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012405321.1 Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.

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CloneID	OSa132365
Clone ID Related Accession (Same CDS sequence)	XM_012549867.1 , XM_023503711.1 , XM_003770117.1
Accession Version	XM_012549867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003839017.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG TGTTTCCCTA CAGAGGGCCG GGGACCCCCG TGCCCGGCCC TCTGGCACCG 
CTGCCCGACT ACATGTCAGA GGAGAAGCTT CAGGAAAAAG CCCGAAAATG GCAGCAGTTG 
CAGGCAAAAC GCTATGCAGA GAAGCGGAAA TTTGGATTTG TAGACGCGCA AAAGGAAGAC 
ATGCCTCCAG AGCACGTCAG GAAGATCATC CGGGACCATG GGGACATGAC GAACCGCAAG 
TTCCGTCATG ACAAGCGAGT CTACCTGGGG GCCCTGAAGT ACATGCCGCA TGCTGTCCTC 
AAGCTCTTGG AAAACATGCC CATGCCCTGG GAGCAGATCC GGGATGTGCC TGTTCTGTAC 
CACATCACTG GTGCGATTTC CTTTGTTAAT GAGATTCCTT GGGTCATCGA GCCTGTCTAC 
ATCTCTCAGT GGGGGTCCAT GTGGATCATG ATGCGTCGAG AGAAGAGGGA CAGGCGGCAC 
TTCAAGCGTA TGCGTTTTCC CCCATTTGAT GATGAGGAGC CGCCCCTTGA CTATGCTGAC 
AACATCCTCG ATGTGGAGCC TTTGGAAGCC ATTCAGCTGG AACTGGACCC TGAGGAGGAT 
GCCCCTGTGC TAGACTGGTT TTATGACCAC CAGCCCTTAA GAGACAGTAG GAAGTACGTG 
AATGGCTCTA CTTATCAGCG CTGGCAGTTC ACCCTGCCCA TGATGTCCAC TCTGTACCGC 
CTGGCCAATC AGCTCCTAAC TGATCTTGTG GATGACAATT ACTTCTATCT GTTTGACCTA 
AAGGCGTTTT TTACCTCTAA GGCCCTCAAC ATGGCTATCC CAGGGGGCCC TAAATTTGAA 
CCGCTGGTCC GAGATATCAA TCTTCAGGAT GAAGACTGGA ATGAATTTAA TGATATCAAC 
AAGATCATCA TCAGGCAGCC CATCAGAACC GAATATAAGA TTGCTTTTCC ATACTTGTAC 
AACAACCTTC CACACCACGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACAGAGG ACCCTGATCT TCCAGCCTTC TACTTTGATC CTTTGATCAA TCCTATCTCC 
CACAGACATT CTGTCAAGAG TCAAGAACCT TTGCCAGATG ATGACGAGGA GTTTGAGCTG 
CCCGAGTTTG TGGAACCTTT CCTCAAGGAC ACGCCCCTTT ACACAGATAA TACTGCCAAT 
GGCATCGCCC TTCTGTGGGC ACCTAGGCCC TTCAACCTTC GCTCTGGTCG CACCCGCCGT 
GCCCTGGACA TACCCCTGGT TAAAAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAGGTGA GGGTCTCCTA CCAAAAGCTA CTCAAATACT ACGTGCTGAA TGCCCTCAAG 
CACCGACCCC CCAAGGCCCA GAAAAAGAGG TATCTCTTCC GATCCTTTAA GGCCACCAAG 
TTCTTCCAGT CTACCAAGCT GGACTGGGTG GAGGTAGGGC TGCAGGTCTG CCGCCAGGGC 
TACAATATGC TCAACCTCCT CATCCACCGG AAGAATCTCA ACTACTTGCA TCTGGACTAT 
AACTTCAACC TAAAGCCTGT CAAGACTCTC ACCACCAAGG AAAGAAAGAA GTCTCGCTTT 
GGAAATGCCT TCCACTTGTG TCGGGAGGTG CTGCGGCTGA CCAAGCTCGT AGTGGACAGC 
CACGTTCAGT ACCGCTTGGG CAACGTGGAT GCCTTCCAGC TAGCGGACGG GCTGCAGTAT 
ATATTTGCCC ACGTGGGACA GCTGACAGGA ATGTACCGCT ACAAGTACAA GTTGATGCGG 
CAGATCCGCA TGTGCAAGGA CCTGAAGCAC CTTATCTACT ACCGCTTCAA CACGGGTCCC 
GTGGGGAAGG GCCCAGGCTG TGGTTTCTGG GCTGCTGGTT GGCGAGTCTG GTTGTTTTTC 
ATGCGTGGCA TCACCCCTCT TTTGGAACGG TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGTC ACTCAAAGGG GGTGGCAAAG ACCGTGACCA AGCAGCGAGT GGAGTCCCAC 
TTTGACTTGG AGCTCCGGGC AGCGGTGATG CATGATATCT TGGATATGAT GCCTGAGGGC 
ATCAAACAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TCCCCTGGAA GGTTCCTGGG TTGCCGACCC CCATTGAGAA CATGATCCTG 
CGCTACGTGA AGGCGAAGGC GGATTGGTGG ACCAACACCG CCCATTACAA CCGAGAGCGG 
ATCCGCCGGG GAGCCACCGT CGACAAGACC GTTTGTAAGA AGAACTTGGG TCGGCTCACA 
CGGCTCTATC TGAAGGCTGA ACAGGAACGA CAGCACAACT ACCTAAAGGA TGGTCCCTAC 
ATCACAGCAG AAGAGGCAGT GGCTGTCTAC ACCACCACTG TGCACTGGCT GGAGAGCCGC 
AGATTCTCCC CCATCCCTTT CCCCCCACTC TCCTACAAGC ATGACACCAA GCTACTCATC 
CTGGCCCTGG AGAGGCTCAA GGAGGCCTAC AGTGTGAAGT CCCGTTTGAA CCAATCCCAA 
AGAGAGGAGC TGGGCCTGAT TGAGCAAGCC TATGACAACC CGCACGAGGC TCTGTCTCGT 
ATCAAGCGTC ACTTACTCAC GCAGAGAGCC TTCAAGGAGG TGGGCATTGA GTTCATGGAC 
CTGTACAGTC ACCTGGTTCC TGTGTACGAC GTAGAACCCC TCGAGAAGAT CACAGACGCC 
TACTTGGACC AGTACCTGTG GTATGAAGCT GACAAGCGCC GGCTCTTCCC ACCCTGGATC 
AAACCTGCTG ACACGGAGCC GCCTCCCCTG CTGGTGTATA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAG GGAGAGTGCA ACGTCATGCT GGAGTCGCGC 
TTTGAGAAGA TGTACGAGAA GATCGACTTG ACGCTCCTGA ACAGGCTCCT GCGCCTCATT 
GTGGACCACA ACATTGCTGA CTATATGACG GCCAAGAACA ACGTGGTCAT CAACTACAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATTCGAGGAC TTCAGTTCGC CTCCTTCATC 
GTGCAGTACT ACGGGCTGGT GATGGACCTT CTGGTATTGG GCCTACACCG GGCCAGCGAG 
ATGGCTGGGC CTCCCCAAAT GCCCAATGAC TTCCTTAGCT TCCAAGACAT CGCCACAGAA 
GCTGCCCACC CCATCCGCCT TTTCTGTAGA TACATCGACC GCATCCACAT TTTCTTCAGG 
TTCACTGCCG ACGAGGCCCG GGACTTGATC CAGCGCTACC TCACGGAACA CCCTGACCCC 
AACAACGAGA ACATCGTGGG CTACAACAAC AAGAAGTGCT GGCCCCGCGA CGCGCGGATG 
CGCCTCATGA AGCATGACGT CAACTTGGGC CGCGCTGTGT TCTGGGACAT CAAAAACCGA 
CTGCCGCGAT CGGTAACAAC GGTGCAGTGG GAGAACAGCT TTGTGTCAGT GTACAGCAAG 
GACAACCCCA ATCTGCTCTT CAACATGTGC GGCTTCGAGT GCCGCATCCT GCCCAAATGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GATGGTGTTT GGAACCTCCA GAATGAGGTC 
ACAAAGGAGC GGACAGCTCA GTGCTTCCTG CGTGTGGATG ATGAGTCTAT GCAGCGCTTC 
CACAACCGTG TGCGTCAGAT CCTGATGGCC TCTGGCTCGA CCACCTTCAC CAAGATTGTG 
AACAAGTGGA ACACGGCTCT CATTGGGCTC ATGACGTACT TCCGTGAGGC TGTGGTGAAC 
ACCCAGGAGC TGCTGGACCT TCTGGTGAAA TGTGAGAACA AGATCCAGAC CCGAATCAAG 
ATCGGGCTCA ACTCCAAGAT GCCAAGCCGC TTCCCTCCAG TCGTGTTCTA CACCCCCAAA 
GAGCTCGGGG GGCTGGGCAT GTTGTCCATG GGCCACGTGC TCATCCCCCA GTCTGATCTC 
AGGTGGTCCA AGCAGACAGA TGTGGGCATC ACCCACTTCC GCTCCGGGAT GAGCCACGAA 
GAAGACCAGC TCATTCCTAA CTTGTACCGT TACATCCAGC CTTGGGAGAG CGAGTTCATT 
GACTCTCAAC GGGTCTGGGC CGAGTATGCG CTCAAGAGGC AGGAAGCCAT CGCTCAGAAC 
AGGCGGCTGA CCTTGGAGGA CCTGGAGGAT TCATGGGATC GAGGCATTCC CCGGATCAAT 
ACCCTGTTCC AAAAGGACCG GCACACACTG GCTTATGACA AGGGCTGGCG TGTCCGCACT 
GACTTCAAGC AATACCAGGT CCTGAAACAG AATCCTTTCT GGTGGACCCA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAATTAC CGCACAGACA TGATCCAGGC CCTGGGTGGT 
GTGGAGGGCA TCCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGT 
CTCTTCTGGG AGAAGGCCAG TGGTTTTGAG GAGTCGATGA AATGGAAGAA GCTGACCAAT 
GCCCAGCGGT CCGGCTTGAA CCAGATCCCC AACCGTCGCT TCACACTGTG GTGGTCGCCA 
ACCATTAACC GCGCCAATGT GTATGTAGGT TTCCAGGTCC AGCTGGACCT TACTGGCATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTCA TCCAGATTTT CCGTGCCCAT 
TTATGGCAGA AGATCCATGA GAGTATTGTC ATGGACTTGT GTCAGGTGTT TGACCAAGAA 
TTGGATGCGC TGGAAATTGA GACGGTGCAG AAGGAGACAA TTCACCCCCG GAAGTCCTAT 
AAGATGAACT CATCTTGTGC AGACATTCTG CTTTTTGCCT CCTACAAATG GAACGTCTCC 
CGGCCGTCTC TGCTGGCTGA CTCCAAGGAC GTGATGGACA GCACCACCAC TCAGAAGTAC 
TGGATCGACA TCCAGCTGCG CTGGGGCGAC TATGACTCCC ACGATATCGA ACGCTACGCT 
CGGGCCAAGT TTCTGGATTA CACTACCGAC AACATGAGTA TCTACCCCTC TCCAACGGGG 
GTGCTCATTG CCATAGACCT GGCATACAAT CTGCACAGCG CCTATGGAAA CTGGTTTCCA 
GGCAGCAAGC CGCTGATCCA GCAAGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCTTG 
TACGTGCTCC GAGAGCGAAT ACGCAAGGGG TTGCAGCTGT ACTCCTCCGA GCCCACAGAG 
CCTTACCTCT CCTCCCAGAA CTATGGCGAG CTCTTCTCCA ACCAGATCAT CTGGTTTGTA 
GATGACACCA ATGTCTACAG AGTCACCATT CACAAGACTT TTGAAGGGAA CTTGACTACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AATCCAAGGA CGGGGCAGCT CTTCCTGAAG 
ATCATCCACA CGTCTGTGTG GGCTGGACAG AAGCGTCTGG GTCAGTTGGC CAAGTGGAAG 
ACAGCCGAGG AAGTGGCTGC CCTGATCCGT TCTCTCCCTG TAGAAGAGCA GCCCAAACAG 
ATTATTGTGA CCAGAAAAGG CATGTTGGAC CCCTTGGAGG TGCACTTACT GGACTTCCCC 
AACATTGTGA TCAAAGGCTC GGAGCTGCAG CTGCCCTTCC AGGCCTGTCT CAAAGTGGAA 
AAATTCGGGG ACCTTATCCT CAAGGCCACC GAGCCCCAAA TGGTTCTCTT CAATCTCTAT 
GACGACTGGC TCAGAACCAT CTCATCCTAC ACGGCCTTTT CCCGTCTGAT CCTGATTCTC 
CGTGCCCTTC ATGTGAACAA TGACCGGGCC AAAGTGATCT TGAAGCCAGA CAAGACCACC 
ATCACAGAGC CGCACCACAT TTGGCCCACG CTGACCGACG AGGAGTGGAT CAAGGTTGAG 
GTGCAGCTCA AGGACTTGAT CCTGGCTGAT TACGGCAAAA AGAACAACGT CAACGTGGCC 
TCACTGACAC AATCTGAGAT CCGGGACATC ATTCTGGGCA TGGAAATCTC CGCCCCTTCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAGCAGTC CCAGCTGACG 
GCAACACAGA CCCGGACTGT CAACAAGCAC GGTGATGAGA TCATCACTTC CACCACCAGC 
AACTACGAGA CACAAACTTT TTCCTCGAAG ACCGAGTGGA GGGTCAGGGC CATCTCTGCA 
GCCAACCTGC ATCTGCGGAC CAACCACATC TACGTGTCAT CTGATGACAT CAAAGAGACA 
GGCTACACTT ACATCCTTCC CAAGAATGTC CTCAAGAAAT TCATCTGCAT TTCTGATCTT 
CGGGCCCAGA TTGCAGGCTA CCTCTATGGA GTGAGCCCCC CGGACAACCC TCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTGCCTCAG TGGGGCACCC ACCAGACGGT GCACCTGCCG 
GGCCAGCTTC CCCAACACGA GTACCTCAAG GAGATGGAGC CCCTCGGCTG GATCCACACA 
CAGCCCAACG AGTCCCCCCA GCTCTCGCCC CAGGATGTCA CCACCCATGC CAAGGTCATG 
GCCGACAACC CCTCATGGGA CGGGGAGAAG ACAATCATCA TCACCTGCAG CTTTACCCCG 
GGCTCTTGCA CCCTGACGGC CTACAAGCTG ACGCCCAGTG GTTACGAGTG GGGTCGGCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACTTGCCTT CCCACTATGA GCGTGTCCAG 
ATGCTGCTCT CTGACCGCTT CCTTGGCTTC TTCATGGTCC CCGCTCAGGC GTCCTGGAAC 
TACAATTTCA TGGGTGTTCG GCACGACCCC AACATGAAAT ACGAGCTGCA GCTGGCCAAC 
CCCAAAGAGT TCTATCACGA GGTGCACCGG CCCTCCCACT TCCTGAACTT CGCGCTGCTG 
CAGGAGGGGG AGGTGTACTC GGCCGACCGA AAGGACCTGT ACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012405321.1
CDS	71..7078
Translation	MAGVFPYRGPGTPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLRTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKVMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQASWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADRKDLYA

Target ORF information:

RefSeq Version	XM_012549867.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012549867.1

ORF Insert Sequence:

ATGGCCGGGG TGTTTCCCTA CAGAGGGCCG GGGACCCCCG TGCCCGGCCC TCTGGCACCG 
CTGCCCGACT ACATGTCAGA GGAGAAGCTT CAGGAAAAAG CCCGAAAATG GCAGCAGTTG 
CAGGCAAAAC GCTATGCAGA GAAGCGGAAA TTTGGATTTG TAGACGCGCA AAAGGAAGAC 
ATGCCTCCAG AGCACGTCAG GAAGATCATC CGGGACCATG GGGACATGAC GAACCGCAAG 
TTCCGTCATG ACAAGCGAGT CTACCTGGGG GCCCTGAAGT ACATGCCGCA TGCTGTCCTC 
AAGCTCTTGG AAAACATGCC CATGCCCTGG GAGCAGATCC GGGATGTGCC TGTTCTGTAC 
CACATCACTG GTGCGATTTC CTTTGTTAAT GAGATTCCTT GGGTCATCGA GCCTGTCTAC 
ATCTCTCAGT GGGGGTCCAT GTGGATCATG ATGCGTCGAG AGAAGAGGGA CAGGCGGCAC 
TTCAAGCGTA TGCGTTTTCC CCCATTTGAT GATGAGGAGC CGCCCCTTGA CTATGCTGAC 
AACATCCTCG ATGTGGAGCC TTTGGAAGCC ATTCAGCTGG AACTGGACCC TGAGGAGGAT 
GCCCCTGTGC TAGACTGGTT TTATGACCAC CAGCCCTTAA GAGACAGTAG GAAGTACGTG 
AATGGCTCTA CTTATCAGCG CTGGCAGTTC ACCCTGCCCA TGATGTCCAC TCTGTACCGC 
CTGGCCAATC AGCTCCTAAC TGATCTTGTG GATGACAATT ACTTCTATCT GTTTGACCTA 
AAGGCGTTTT TTACCTCTAA GGCCCTCAAC ATGGCTATCC CAGGGGGCCC TAAATTTGAA 
CCGCTGGTCC GAGATATCAA TCTTCAGGAT GAAGACTGGA ATGAATTTAA TGATATCAAC 
AAGATCATCA TCAGGCAGCC CATCAGAACC GAATATAAGA TTGCTTTTCC ATACTTGTAC 
AACAACCTTC CACACCACGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACAGAGG ACCCTGATCT TCCAGCCTTC TACTTTGATC CTTTGATCAA TCCTATCTCC 
CACAGACATT CTGTCAAGAG TCAAGAACCT TTGCCAGATG ATGACGAGGA GTTTGAGCTG 
CCCGAGTTTG TGGAACCTTT CCTCAAGGAC ACGCCCCTTT ACACAGATAA TACTGCCAAT 
GGCATCGCCC TTCTGTGGGC ACCTAGGCCC TTCAACCTTC GCTCTGGTCG CACCCGCCGT 
GCCCTGGACA TACCCCTGGT TAAAAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAGGTGA GGGTCTCCTA CCAAAAGCTA CTCAAATACT ACGTGCTGAA TGCCCTCAAG 
CACCGACCCC CCAAGGCCCA GAAAAAGAGG TATCTCTTCC GATCCTTTAA GGCCACCAAG 
TTCTTCCAGT CTACCAAGCT GGACTGGGTG GAGGTAGGGC TGCAGGTCTG CCGCCAGGGC 
TACAATATGC TCAACCTCCT CATCCACCGG AAGAATCTCA ACTACTTGCA TCTGGACTAT 
AACTTCAACC TAAAGCCTGT CAAGACTCTC ACCACCAAGG AAAGAAAGAA GTCTCGCTTT 
GGAAATGCCT TCCACTTGTG TCGGGAGGTG CTGCGGCTGA CCAAGCTCGT AGTGGACAGC 
CACGTTCAGT ACCGCTTGGG CAACGTGGAT GCCTTCCAGC TAGCGGACGG GCTGCAGTAT 
ATATTTGCCC ACGTGGGACA GCTGACAGGA ATGTACCGCT ACAAGTACAA GTTGATGCGG 
CAGATCCGCA TGTGCAAGGA CCTGAAGCAC CTTATCTACT ACCGCTTCAA CACGGGTCCC 
GTGGGGAAGG GCCCAGGCTG TGGTTTCTGG GCTGCTGGTT GGCGAGTCTG GTTGTTTTTC 
ATGCGTGGCA TCACCCCTCT TTTGGAACGG TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGTC ACTCAAAGGG GGTGGCAAAG ACCGTGACCA AGCAGCGAGT GGAGTCCCAC 
TTTGACTTGG AGCTCCGGGC AGCGGTGATG CATGATATCT TGGATATGAT GCCTGAGGGC 
ATCAAACAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TCCCCTGGAA GGTTCCTGGG TTGCCGACCC CCATTGAGAA CATGATCCTG 
CGCTACGTGA AGGCGAAGGC GGATTGGTGG ACCAACACCG CCCATTACAA CCGAGAGCGG 
ATCCGCCGGG GAGCCACCGT CGACAAGACC GTTTGTAAGA AGAACTTGGG TCGGCTCACA 
CGGCTCTATC TGAAGGCTGA ACAGGAACGA CAGCACAACT ACCTAAAGGA TGGTCCCTAC 
ATCACAGCAG AAGAGGCAGT GGCTGTCTAC ACCACCACTG TGCACTGGCT GGAGAGCCGC 
AGATTCTCCC CCATCCCTTT CCCCCCACTC TCCTACAAGC ATGACACCAA GCTACTCATC 
CTGGCCCTGG AGAGGCTCAA GGAGGCCTAC AGTGTGAAGT CCCGTTTGAA CCAATCCCAA 
AGAGAGGAGC TGGGCCTGAT TGAGCAAGCC TATGACAACC CGCACGAGGC TCTGTCTCGT 
ATCAAGCGTC ACTTACTCAC GCAGAGAGCC TTCAAGGAGG TGGGCATTGA GTTCATGGAC 
CTGTACAGTC ACCTGGTTCC TGTGTACGAC GTAGAACCCC TCGAGAAGAT CACAGACGCC 
TACTTGGACC AGTACCTGTG GTATGAAGCT GACAAGCGCC GGCTCTTCCC ACCCTGGATC 
AAACCTGCTG ACACGGAGCC GCCTCCCCTG CTGGTGTATA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAG GGAGAGTGCA ACGTCATGCT GGAGTCGCGC 
TTTGAGAAGA TGTACGAGAA GATCGACTTG ACGCTCCTGA ACAGGCTCCT GCGCCTCATT 
GTGGACCACA ACATTGCTGA CTATATGACG GCCAAGAACA ACGTGGTCAT CAACTACAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATTCGAGGAC TTCAGTTCGC CTCCTTCATC 
GTGCAGTACT ACGGGCTGGT GATGGACCTT CTGGTATTGG GCCTACACCG GGCCAGCGAG 
ATGGCTGGGC CTCCCCAAAT GCCCAATGAC TTCCTTAGCT TCCAAGACAT CGCCACAGAA 
GCTGCCCACC CCATCCGCCT TTTCTGTAGA TACATCGACC GCATCCACAT TTTCTTCAGG 
TTCACTGCCG ACGAGGCCCG GGACTTGATC CAGCGCTACC TCACGGAACA CCCTGACCCC 
AACAACGAGA ACATCGTGGG CTACAACAAC AAGAAGTGCT GGCCCCGCGA CGCGCGGATG 
CGCCTCATGA AGCATGACGT CAACTTGGGC CGCGCTGTGT TCTGGGACAT CAAAAACCGA 
CTGCCGCGAT CGGTAACAAC GGTGCAGTGG GAGAACAGCT TTGTGTCAGT GTACAGCAAG 
GACAACCCCA ATCTGCTCTT CAACATGTGC GGCTTCGAGT GCCGCATCCT GCCCAAATGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GATGGTGTTT GGAACCTCCA GAATGAGGTC 
ACAAAGGAGC GGACAGCTCA GTGCTTCCTG CGTGTGGATG ATGAGTCTAT GCAGCGCTTC 
CACAACCGTG TGCGTCAGAT CCTGATGGCC TCTGGCTCGA CCACCTTCAC CAAGATTGTG 
AACAAGTGGA ACACGGCTCT CATTGGGCTC ATGACGTACT TCCGTGAGGC TGTGGTGAAC 
ACCCAGGAGC TGCTGGACCT TCTGGTGAAA TGTGAGAACA AGATCCAGAC CCGAATCAAG 
ATCGGGCTCA ACTCCAAGAT GCCAAGCCGC TTCCCTCCAG TCGTGTTCTA CACCCCCAAA 
GAGCTCGGGG GGCTGGGCAT GTTGTCCATG GGCCACGTGC TCATCCCCCA GTCTGATCTC 
AGGTGGTCCA AGCAGACAGA TGTGGGCATC ACCCACTTCC GCTCCGGGAT GAGCCACGAA 
GAAGACCAGC TCATTCCTAA CTTGTACCGT TACATCCAGC CTTGGGAGAG CGAGTTCATT 
GACTCTCAAC GGGTCTGGGC CGAGTATGCG CTCAAGAGGC AGGAAGCCAT CGCTCAGAAC 
AGGCGGCTGA CCTTGGAGGA CCTGGAGGAT TCATGGGATC GAGGCATTCC CCGGATCAAT 
ACCCTGTTCC AAAAGGACCG GCACACACTG GCTTATGACA AGGGCTGGCG TGTCCGCACT 
GACTTCAAGC AATACCAGGT CCTGAAACAG AATCCTTTCT GGTGGACCCA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAATTAC CGCACAGACA TGATCCAGGC CCTGGGTGGT 
GTGGAGGGCA TCCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGT 
CTCTTCTGGG AGAAGGCCAG TGGTTTTGAG GAGTCGATGA AATGGAAGAA GCTGACCAAT 
GCCCAGCGGT CCGGCTTGAA CCAGATCCCC AACCGTCGCT TCACACTGTG GTGGTCGCCA 
ACCATTAACC GCGCCAATGT GTATGTAGGT TTCCAGGTCC AGCTGGACCT TACTGGCATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTCA TCCAGATTTT CCGTGCCCAT 
TTATGGCAGA AGATCCATGA GAGTATTGTC ATGGACTTGT GTCAGGTGTT TGACCAAGAA 
TTGGATGCGC TGGAAATTGA GACGGTGCAG AAGGAGACAA TTCACCCCCG GAAGTCCTAT 
AAGATGAACT CATCTTGTGC AGACATTCTG CTTTTTGCCT CCTACAAATG GAACGTCTCC 
CGGCCGTCTC TGCTGGCTGA CTCCAAGGAC GTGATGGACA GCACCACCAC TCAGAAGTAC 
TGGATCGACA TCCAGCTGCG CTGGGGCGAC TATGACTCCC ACGATATCGA ACGCTACGCT 
CGGGCCAAGT TTCTGGATTA CACTACCGAC AACATGAGTA TCTACCCCTC TCCAACGGGG 
GTGCTCATTG CCATAGACCT GGCATACAAT CTGCACAGCG CCTATGGAAA CTGGTTTCCA 
GGCAGCAAGC CGCTGATCCA GCAAGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCTTG 
TACGTGCTCC GAGAGCGAAT ACGCAAGGGG TTGCAGCTGT ACTCCTCCGA GCCCACAGAG 
CCTTACCTCT CCTCCCAGAA CTATGGCGAG CTCTTCTCCA ACCAGATCAT CTGGTTTGTA 
GATGACACCA ATGTCTACAG AGTCACCATT CACAAGACTT TTGAAGGGAA CTTGACTACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AATCCAAGGA CGGGGCAGCT CTTCCTGAAG 
ATCATCCACA CGTCTGTGTG GGCTGGACAG AAGCGTCTGG GTCAGTTGGC CAAGTGGAAG 
ACAGCCGAGG AAGTGGCTGC CCTGATCCGT TCTCTCCCTG TAGAAGAGCA GCCCAAACAG 
ATTATTGTGA CCAGAAAAGG CATGTTGGAC CCCTTGGAGG TGCACTTACT GGACTTCCCC 
AACATTGTGA TCAAAGGCTC GGAGCTGCAG CTGCCCTTCC AGGCCTGTCT CAAAGTGGAA 
AAATTCGGGG ACCTTATCCT CAAGGCCACC GAGCCCCAAA TGGTTCTCTT CAATCTCTAT 
GACGACTGGC TCAGAACCAT CTCATCCTAC ACGGCCTTTT CCCGTCTGAT CCTGATTCTC 
CGTGCCCTTC ATGTGAACAA TGACCGGGCC AAAGTGATCT TGAAGCCAGA CAAGACCACC 
ATCACAGAGC CGCACCACAT TTGGCCCACG CTGACCGACG AGGAGTGGAT CAAGGTTGAG 
GTGCAGCTCA AGGACTTGAT CCTGGCTGAT TACGGCAAAA AGAACAACGT CAACGTGGCC 
TCACTGACAC AATCTGAGAT CCGGGACATC ATTCTGGGCA TGGAAATCTC CGCCCCTTCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAGCAGTC CCAGCTGACG 
GCAACACAGA CCCGGACTGT CAACAAGCAC GGTGATGAGA TCATCACTTC CACCACCAGC 
AACTACGAGA CACAAACTTT TTCCTCGAAG ACCGAGTGGA GGGTCAGGGC CATCTCTGCA 
GCCAACCTGC ATCTGCGGAC CAACCACATC TACGTGTCAT CTGATGACAT CAAAGAGACA 
GGCTACACTT ACATCCTTCC CAAGAATGTC CTCAAGAAAT TCATCTGCAT TTCTGATCTT 
CGGGCCCAGA TTGCAGGCTA CCTCTATGGA GTGAGCCCCC CGGACAACCC TCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTGCCTCAG TGGGGCACCC ACCAGACGGT GCACCTGCCG 
GGCCAGCTTC CCCAACACGA GTACCTCAAG GAGATGGAGC CCCTCGGCTG GATCCACACA 
CAGCCCAACG AGTCCCCCCA GCTCTCGCCC CAGGATGTCA CCACCCATGC CAAGGTCATG 
GCCGACAACC CCTCATGGGA CGGGGAGAAG ACAATCATCA TCACCTGCAG CTTTACCCCG 
GGCTCTTGCA CCCTGACGGC CTACAAGCTG ACGCCCAGTG GTTACGAGTG GGGTCGGCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACTTGCCTT CCCACTATGA GCGTGTCCAG 
ATGCTGCTCT CTGACCGCTT CCTTGGCTTC TTCATGGTCC CCGCTCAGGC GTCCTGGAAC 
TACAATTTCA TGGGTGTTCG GCACGACCCC AACATGAAAT ACGAGCTGCA GCTGGCCAAC 
CCCAAAGAGT TCTATCACGA GGTGCACCGG CCCTCCCACT TCCTGAACTT CGCGCTGCTG 
CAGGAGGGGG AGGTGTACTC GGCCGACCGA AAGGACCTGT ACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF8 ( XM_023503711.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023359479.1 Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), mRNA.

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CloneID	OSa132365
Clone ID Related Accession (Same CDS sequence)	XM_012549867.1 , XM_023503711.1 , XM_003770117.1
Accession Version	XM_023503711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003839017.1) and transcript sequence (GEDN01152882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 135 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCCGGGG TGTTTCCCTA CAGAGGGCCG GGGACCCCCG TGCCCGGCCC TCTGGCACCG 
CTGCCCGACT ACATGTCAGA GGAGAAGCTT CAGGAAAAAG CCCGAAAATG GCAGCAGTTG 
CAGGCAAAAC GCTATGCAGA GAAGCGGAAA TTTGGATTTG TAGACGCGCA AAAGGAAGAC 
ATGCCTCCAG AGCACGTCAG GAAGATCATC CGGGACCATG GGGACATGAC GAACCGCAAG 
TTCCGTCATG ACAAGCGAGT CTACCTGGGG GCCCTGAAGT ACATGCCGCA TGCTGTCCTC 
AAGCTCTTGG AAAACATGCC CATGCCCTGG GAGCAGATCC GGGATGTGCC TGTTCTGTAC 
CACATCACTG GTGCGATTTC CTTTGTTAAT GAGATTCCTT GGGTCATCGA GCCTGTCTAC 
ATCTCTCAGT GGGGGTCCAT GTGGATCATG ATGCGTCGAG AGAAGAGGGA CAGGCGGCAC 
TTCAAGCGTA TGCGTTTTCC CCCATTTGAT GATGAGGAGC CGCCCCTTGA CTATGCTGAC 
AACATCCTCG ATGTGGAGCC TTTGGAAGCC ATTCAGCTGG AACTGGACCC TGAGGAGGAT 
GCCCCTGTGC TAGACTGGTT TTATGACCAC CAGCCCTTAA GAGACAGTAG GAAGTACGTG 
AATGGCTCTA CTTATCAGCG CTGGCAGTTC ACCCTGCCCA TGATGTCCAC TCTGTACCGC 
CTGGCCAATC AGCTCCTAAC TGATCTTGTG GATGACAATT ACTTCTATCT GTTTGACCTA 
AAGGCGTTTT TTACCTCTAA GGCCCTCAAC ATGGCTATCC CAGGGGGCCC TAAATTTGAA 
CCGCTGGTCC GAGATATCAA TCTTCAGGAT GAAGACTGGA ATGAATTTAA TGATATCAAC 
AAGATCATCA TCAGGCAGCC CATCAGAACC GAATATAAGA TTGCTTTTCC ATACTTGTAC 
AACAACCTTC CACACCACGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACAGAGG ACCCTGATCT TCCAGCCTTC TACTTTGATC CTTTGATCAA TCCTATCTCC 
CACAGACATT CTGTCAAGAG TCAAGAACCT TTGCCAGATG ATGACGAGGA GTTTGAGCTG 
CCCGAGTTTG TGGAACCTTT CCTCAAGGAC ACGCCCCTTT ACACAGATAA TACTGCCAAT 
GGCATCGCCC TTCTGTGGGC ACCTAGGCCC TTCAACCTTC GCTCTGGTCG CACCCGCCGT 
GCCCTGGACA TACCCCTGGT TAAAAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAGGTGA GGGTCTCCTA CCAAAAGCTA CTCAAATACT ACGTGCTGAA TGCCCTCAAG 
CACCGACCCC CCAAGGCCCA GAAAAAGAGG TATCTCTTCC GATCCTTTAA GGCCACCAAG 
TTCTTCCAGT CTACCAAGCT GGACTGGGTG GAGGTAGGGC TGCAGGTCTG CCGCCAGGGC 
TACAATATGC TCAACCTCCT CATCCACCGG AAGAATCTCA ACTACTTGCA TCTGGACTAT 
AACTTCAACC TAAAGCCTGT CAAGACTCTC ACCACCAAGG AAAGAAAGAA GTCTCGCTTT 
GGAAATGCCT TCCACTTGTG TCGGGAGGTG CTGCGGCTGA CCAAGCTCGT AGTGGACAGC 
CACGTTCAGT ACCGCTTGGG CAACGTGGAT GCCTTCCAGC TAGCGGACGG GCTGCAGTAT 
ATATTTGCCC ACGTGGGACA GCTGACAGGA ATGTACCGCT ACAAGTACAA GTTGATGCGG 
CAGATCCGCA TGTGCAAGGA CCTGAAGCAC CTTATCTACT ACCGCTTCAA CACGGGTCCC 
GTGGGGAAGG GCCCAGGCTG TGGTTTCTGG GCTGCTGGTT GGCGAGTCTG GTTGTTTTTC 
ATGCGTGGCA TCACCCCTCT TTTGGAACGG TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGTC ACTCAAAGGG GGTGGCAAAG ACCGTGACCA AGCAGCGAGT GGAGTCCCAC 
TTTGACTTGG AGCTCCGGGC AGCGGTGATG CATGATATCT TGGATATGAT GCCTGAGGGC 
ATCAAACAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TCCCCTGGAA GGTTCCTGGG TTGCCGACCC CCATTGAGAA CATGATCCTG 
CGCTACGTGA AGGCGAAGGC GGATTGGTGG ACCAACACCG CCCATTACAA CCGAGAGCGG 
ATCCGCCGGG GAGCCACCGT CGACAAGACC GTTTGTAAGA AGAACTTGGG TCGGCTCACA 
CGGCTCTATC TGAAGGCTGA ACAGGAACGA CAGCACAACT ACCTAAAGGA TGGTCCCTAC 
ATCACAGCAG AAGAGGCAGT GGCTGTCTAC ACCACCACTG TGCACTGGCT GGAGAGCCGC 
AGATTCTCCC CCATCCCTTT CCCCCCACTC TCCTACAAGC ATGACACCAA GCTACTCATC 
CTGGCCCTGG AGAGGCTCAA GGAGGCCTAC AGTGTGAAGT CCCGTTTGAA CCAATCCCAA 
AGAGAGGAGC TGGGCCTGAT TGAGCAAGCC TATGACAACC CGCACGAGGC TCTGTCTCGT 
ATCAAGCGTC ACTTACTCAC GCAGAGAGCC TTCAAGGAGG TGGGCATTGA GTTCATGGAC 
CTGTACAGTC ACCTGGTTCC TGTGTACGAC GTAGAACCCC TCGAGAAGAT CACAGACGCC 
TACTTGGACC AGTACCTGTG GTATGAAGCT GACAAGCGCC GGCTCTTCCC ACCCTGGATC 
AAACCTGCTG ACACGGAGCC GCCTCCCCTG CTGGTGTATA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAG GGAGAGTGCA ACGTCATGCT GGAGTCGCGC 
TTTGAGAAGA TGTACGAGAA GATCGACTTG ACGCTCCTGA ACAGGCTCCT GCGCCTCATT 
GTGGACCACA ACATTGCTGA CTATATGACG GCCAAGAACA ACGTGGTCAT CAACTACAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATTCGAGGAC TTCAGTTCGC CTCCTTCATC 
GTGCAGTACT ACGGGCTGGT GATGGACCTT CTGGTATTGG GCCTACACCG GGCCAGCGAG 
ATGGCTGGGC CTCCCCAAAT GCCCAATGAC TTCCTTAGCT TCCAAGACAT CGCCACAGAA 
GCTGCCCACC CCATCCGCCT TTTCTGTAGA TACATCGACC GCATCCACAT TTTCTTCAGG 
TTCACTGCCG ACGAGGCCCG GGACTTGATC CAGCGCTACC TCACGGAACA CCCTGACCCC 
AACAACGAGA ACATCGTGGG CTACAACAAC AAGAAGTGCT GGCCCCGCGA CGCGCGGATG 
CGCCTCATGA AGCATGACGT CAACTTGGGC CGCGCTGTGT TCTGGGACAT CAAAAACCGA 
CTGCCGCGAT CGGTAACAAC GGTGCAGTGG GAGAACAGCT TTGTGTCAGT GTACAGCAAG 
GACAACCCCA ATCTGCTCTT CAACATGTGC GGCTTCGAGT GCCGCATCCT GCCCAAATGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GATGGTGTTT GGAACCTCCA GAATGAGGTC 
ACAAAGGAGC GGACAGCTCA GTGCTTCCTG CGTGTGGATG ATGAGTCTAT GCAGCGCTTC 
CACAACCGTG TGCGTCAGAT CCTGATGGCC TCTGGCTCGA CCACCTTCAC CAAGATTGTG 
AACAAGTGGA ACACGGCTCT CATTGGGCTC ATGACGTACT TCCGTGAGGC TGTGGTGAAC 
ACCCAGGAGC TGCTGGACCT TCTGGTGAAA TGTGAGAACA AGATCCAGAC CCGAATCAAG 
ATCGGGCTCA ACTCCAAGAT GCCAAGCCGC TTCCCTCCAG TCGTGTTCTA CACCCCCAAA 
GAGCTCGGGG GGCTGGGCAT GTTGTCCATG GGCCACGTGC TCATCCCCCA GTCTGATCTC 
AGGTGGTCCA AGCAGACAGA TGTGGGCATC ACCCACTTCC GCTCCGGGAT GAGCCACGAA 
GAAGACCAGC TCATTCCTAA CTTGTACCGT TACATCCAGC CTTGGGAGAG CGAGTTCATT 
GACTCTCAAC GGGTCTGGGC CGAGTATGCG CTCAAGAGGC AGGAAGCCAT CGCTCAGAAC 
AGGCGGCTGA CCTTGGAGGA CCTGGAGGAT TCATGGGATC GAGGCATTCC CCGGATCAAT 
ACCCTGTTCC AAAAGGACCG GCACACACTG GCTTATGACA AGGGCTGGCG TGTCCGCACT 
GACTTCAAGC AATACCAGGT CCTGAAACAG AATCCTTTCT GGTGGACCCA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAATTAC CGCACAGACA TGATCCAGGC CCTGGGTGGT 
GTGGAGGGCA TCCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGT 
CTCTTCTGGG AGAAGGCCAG TGGTTTTGAG GAGTCGATGA AATGGAAGAA GCTGACCAAT 
GCCCAGCGGT CCGGCTTGAA CCAGATCCCC AACCGTCGCT TCACACTGTG GTGGTCGCCA 
ACCATTAACC GCGCCAATGT GTATGTAGGT TTCCAGGTCC AGCTGGACCT TACTGGCATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTCA TCCAGATTTT CCGTGCCCAT 
TTATGGCAGA AGATCCATGA GAGTATTGTC ATGGACTTGT GTCAGGTGTT TGACCAAGAA 
TTGGATGCGC TGGAAATTGA GACGGTGCAG AAGGAGACAA TTCACCCCCG GAAGTCCTAT 
AAGATGAACT CATCTTGTGC AGACATTCTG CTTTTTGCCT CCTACAAATG GAACGTCTCC 
CGGCCGTCTC TGCTGGCTGA CTCCAAGGAC GTGATGGACA GCACCACCAC TCAGAAGTAC 
TGGATCGACA TCCAGCTGCG CTGGGGCGAC TATGACTCCC ACGATATCGA ACGCTACGCT 
CGGGCCAAGT TTCTGGATTA CACTACCGAC AACATGAGTA TCTACCCCTC TCCAACGGGG 
GTGCTCATTG CCATAGACCT GGCATACAAT CTGCACAGCG CCTATGGAAA CTGGTTTCCA 
GGCAGCAAGC CGCTGATCCA GCAAGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCTTG 
TACGTGCTCC GAGAGCGAAT ACGCAAGGGG TTGCAGCTGT ACTCCTCCGA GCCCACAGAG 
CCTTACCTCT CCTCCCAGAA CTATGGCGAG CTCTTCTCCA ACCAGATCAT CTGGTTTGTA 
GATGACACCA ATGTCTACAG AGTCACCATT CACAAGACTT TTGAAGGGAA CTTGACTACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AATCCAAGGA CGGGGCAGCT CTTCCTGAAG 
ATCATCCACA CGTCTGTGTG GGCTGGACAG AAGCGTCTGG GTCAGTTGGC CAAGTGGAAG 
ACAGCCGAGG AAGTGGCTGC CCTGATCCGT TCTCTCCCTG TAGAAGAGCA GCCCAAACAG 
ATTATTGTGA CCAGAAAAGG CATGTTGGAC CCCTTGGAGG TGCACTTACT GGACTTCCCC 
AACATTGTGA TCAAAGGCTC GGAGCTGCAG CTGCCCTTCC AGGCCTGTCT CAAAGTGGAA 
AAATTCGGGG ACCTTATCCT CAAGGCCACC GAGCCCCAAA TGGTTCTCTT CAATCTCTAT 
GACGACTGGC TCAGAACCAT CTCATCCTAC ACGGCCTTTT CCCGTCTGAT CCTGATTCTC 
CGTGCCCTTC ATGTGAACAA TGACCGGGCC AAAGTGATCT TGAAGCCAGA CAAGACCACC 
ATCACAGAGC CGCACCACAT TTGGCCCACG CTGACCGACG AGGAGTGGAT CAAGGTTGAG 
GTGCAGCTCA AGGACTTGAT CCTGGCTGAT TACGGCAAAA AGAACAACGT CAACGTGGCC 
TCACTGACAC AATCTGAGAT CCGGGACATC ATTCTGGGCA TGGAAATCTC CGCCCCTTCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAGCAGTC CCAGCTGACG 
GCAACACAGA CCCGGACTGT CAACAAGCAC GGTGATGAGA TCATCACTTC CACCACCAGC 
AACTACGAGA CACAAACTTT TTCCTCGAAG ACCGAGTGGA GGGTCAGGGC CATCTCTGCA 
GCCAACCTGC ATCTGCGGAC CAACCACATC TACGTGTCAT CTGATGACAT CAAAGAGACA 
GGCTACACTT ACATCCTTCC CAAGAATGTC CTCAAGAAAT TCATCTGCAT TTCTGATCTT 
CGGGCCCAGA TTGCAGGCTA CCTCTATGGA GTGAGCCCCC CGGACAACCC TCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTGCCTCAG TGGGGCACCC ACCAGACGGT GCACCTGCCG 
GGCCAGCTTC CCCAACACGA GTACCTCAAG GAGATGGAGC CCCTCGGCTG GATCCACACA 
CAGCCCAACG AGTCCCCCCA GCTCTCGCCC CAGGATGTCA CCACCCATGC CAAGGTCATG 
GCCGACAACC CCTCATGGGA CGGGGAGAAG ACAATCATCA TCACCTGCAG CTTTACCCCG 
GGCTCTTGCA CCCTGACGGC CTACAAGCTG ACGCCCAGTG GTTACGAGTG GGGTCGGCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACTTGCCTT CCCACTATGA GCGTGTCCAG 
ATGCTGCTCT CTGACCGCTT CCTTGGCTTC TTCATGGTCC CCGCTCAGGC GTCCTGGAAC 
TACAATTTCA TGGGTGTTCG GCACGACCCC AACATGAAAT ACGAGCTGCA GCTGGCCAAC 
CCCAAAGAGT TCTATCACGA GGTGCACCGG CCCTCCCACT TCCTGAACTT CGCGCTGCTG 
CAGGAGGGGG AGGTGTACTC GGCCGACCGA AAGGACCTGT ACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023359479.1
CDS	1..7008
Translation	MAGVFPYRGPGTPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLRTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKVMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQASWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADRKDLYA

Target ORF information:

RefSeq Version	XM_023503711.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023503711.1

ORF Insert Sequence:

ATGGCCGGGG TGTTTCCCTA CAGAGGGCCG GGGACCCCCG TGCCCGGCCC TCTGGCACCG 
CTGCCCGACT ACATGTCAGA GGAGAAGCTT CAGGAAAAAG CCCGAAAATG GCAGCAGTTG 
CAGGCAAAAC GCTATGCAGA GAAGCGGAAA TTTGGATTTG TAGACGCGCA AAAGGAAGAC 
ATGCCTCCAG AGCACGTCAG GAAGATCATC CGGGACCATG GGGACATGAC GAACCGCAAG 
TTCCGTCATG ACAAGCGAGT CTACCTGGGG GCCCTGAAGT ACATGCCGCA TGCTGTCCTC 
AAGCTCTTGG AAAACATGCC CATGCCCTGG GAGCAGATCC GGGATGTGCC TGTTCTGTAC 
CACATCACTG GTGCGATTTC CTTTGTTAAT GAGATTCCTT GGGTCATCGA GCCTGTCTAC 
ATCTCTCAGT GGGGGTCCAT GTGGATCATG ATGCGTCGAG AGAAGAGGGA CAGGCGGCAC 
TTCAAGCGTA TGCGTTTTCC CCCATTTGAT GATGAGGAGC CGCCCCTTGA CTATGCTGAC 
AACATCCTCG ATGTGGAGCC TTTGGAAGCC ATTCAGCTGG AACTGGACCC TGAGGAGGAT 
GCCCCTGTGC TAGACTGGTT TTATGACCAC CAGCCCTTAA GAGACAGTAG GAAGTACGTG 
AATGGCTCTA CTTATCAGCG CTGGCAGTTC ACCCTGCCCA TGATGTCCAC TCTGTACCGC 
CTGGCCAATC AGCTCCTAAC TGATCTTGTG GATGACAATT ACTTCTATCT GTTTGACCTA 
AAGGCGTTTT TTACCTCTAA GGCCCTCAAC ATGGCTATCC CAGGGGGCCC TAAATTTGAA 
CCGCTGGTCC GAGATATCAA TCTTCAGGAT GAAGACTGGA ATGAATTTAA TGATATCAAC 
AAGATCATCA TCAGGCAGCC CATCAGAACC GAATATAAGA TTGCTTTTCC ATACTTGTAC 
AACAACCTTC CACACCACGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACAGAGG ACCCTGATCT TCCAGCCTTC TACTTTGATC CTTTGATCAA TCCTATCTCC 
CACAGACATT CTGTCAAGAG TCAAGAACCT TTGCCAGATG ATGACGAGGA GTTTGAGCTG 
CCCGAGTTTG TGGAACCTTT CCTCAAGGAC ACGCCCCTTT ACACAGATAA TACTGCCAAT 
GGCATCGCCC TTCTGTGGGC ACCTAGGCCC TTCAACCTTC GCTCTGGTCG CACCCGCCGT 
GCCCTGGACA TACCCCTGGT TAAAAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAGGTGA GGGTCTCCTA CCAAAAGCTA CTCAAATACT ACGTGCTGAA TGCCCTCAAG 
CACCGACCCC CCAAGGCCCA GAAAAAGAGG TATCTCTTCC GATCCTTTAA GGCCACCAAG 
TTCTTCCAGT CTACCAAGCT GGACTGGGTG GAGGTAGGGC TGCAGGTCTG CCGCCAGGGC 
TACAATATGC TCAACCTCCT CATCCACCGG AAGAATCTCA ACTACTTGCA TCTGGACTAT 
AACTTCAACC TAAAGCCTGT CAAGACTCTC ACCACCAAGG AAAGAAAGAA GTCTCGCTTT 
GGAAATGCCT TCCACTTGTG TCGGGAGGTG CTGCGGCTGA CCAAGCTCGT AGTGGACAGC 
CACGTTCAGT ACCGCTTGGG CAACGTGGAT GCCTTCCAGC TAGCGGACGG GCTGCAGTAT 
ATATTTGCCC ACGTGGGACA GCTGACAGGA ATGTACCGCT ACAAGTACAA GTTGATGCGG 
CAGATCCGCA TGTGCAAGGA CCTGAAGCAC CTTATCTACT ACCGCTTCAA CACGGGTCCC 
GTGGGGAAGG GCCCAGGCTG TGGTTTCTGG GCTGCTGGTT GGCGAGTCTG GTTGTTTTTC 
ATGCGTGGCA TCACCCCTCT TTTGGAACGG TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGTC ACTCAAAGGG GGTGGCAAAG ACCGTGACCA AGCAGCGAGT GGAGTCCCAC 
TTTGACTTGG AGCTCCGGGC AGCGGTGATG CATGATATCT TGGATATGAT GCCTGAGGGC 
ATCAAACAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TCCCCTGGAA GGTTCCTGGG TTGCCGACCC CCATTGAGAA CATGATCCTG 
CGCTACGTGA AGGCGAAGGC GGATTGGTGG ACCAACACCG CCCATTACAA CCGAGAGCGG 
ATCCGCCGGG GAGCCACCGT CGACAAGACC GTTTGTAAGA AGAACTTGGG TCGGCTCACA 
CGGCTCTATC TGAAGGCTGA ACAGGAACGA CAGCACAACT ACCTAAAGGA TGGTCCCTAC 
ATCACAGCAG AAGAGGCAGT GGCTGTCTAC ACCACCACTG TGCACTGGCT GGAGAGCCGC 
AGATTCTCCC CCATCCCTTT CCCCCCACTC TCCTACAAGC ATGACACCAA GCTACTCATC 
CTGGCCCTGG AGAGGCTCAA GGAGGCCTAC AGTGTGAAGT CCCGTTTGAA CCAATCCCAA 
AGAGAGGAGC TGGGCCTGAT TGAGCAAGCC TATGACAACC CGCACGAGGC TCTGTCTCGT 
ATCAAGCGTC ACTTACTCAC GCAGAGAGCC TTCAAGGAGG TGGGCATTGA GTTCATGGAC 
CTGTACAGTC ACCTGGTTCC TGTGTACGAC GTAGAACCCC TCGAGAAGAT CACAGACGCC 
TACTTGGACC AGTACCTGTG GTATGAAGCT GACAAGCGCC GGCTCTTCCC ACCCTGGATC 
AAACCTGCTG ACACGGAGCC GCCTCCCCTG CTGGTGTATA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAG GGAGAGTGCA ACGTCATGCT GGAGTCGCGC 
TTTGAGAAGA TGTACGAGAA GATCGACTTG ACGCTCCTGA ACAGGCTCCT GCGCCTCATT 
GTGGACCACA ACATTGCTGA CTATATGACG GCCAAGAACA ACGTGGTCAT CAACTACAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATTCGAGGAC TTCAGTTCGC CTCCTTCATC 
GTGCAGTACT ACGGGCTGGT GATGGACCTT CTGGTATTGG GCCTACACCG GGCCAGCGAG 
ATGGCTGGGC CTCCCCAAAT GCCCAATGAC TTCCTTAGCT TCCAAGACAT CGCCACAGAA 
GCTGCCCACC CCATCCGCCT TTTCTGTAGA TACATCGACC GCATCCACAT TTTCTTCAGG 
TTCACTGCCG ACGAGGCCCG GGACTTGATC CAGCGCTACC TCACGGAACA CCCTGACCCC 
AACAACGAGA ACATCGTGGG CTACAACAAC AAGAAGTGCT GGCCCCGCGA CGCGCGGATG 
CGCCTCATGA AGCATGACGT CAACTTGGGC CGCGCTGTGT TCTGGGACAT CAAAAACCGA 
CTGCCGCGAT CGGTAACAAC GGTGCAGTGG GAGAACAGCT TTGTGTCAGT GTACAGCAAG 
GACAACCCCA ATCTGCTCTT CAACATGTGC GGCTTCGAGT GCCGCATCCT GCCCAAATGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GATGGTGTTT GGAACCTCCA GAATGAGGTC 
ACAAAGGAGC GGACAGCTCA GTGCTTCCTG CGTGTGGATG ATGAGTCTAT GCAGCGCTTC 
CACAACCGTG TGCGTCAGAT CCTGATGGCC TCTGGCTCGA CCACCTTCAC CAAGATTGTG 
AACAAGTGGA ACACGGCTCT CATTGGGCTC ATGACGTACT TCCGTGAGGC TGTGGTGAAC 
ACCCAGGAGC TGCTGGACCT TCTGGTGAAA TGTGAGAACA AGATCCAGAC CCGAATCAAG 
ATCGGGCTCA ACTCCAAGAT GCCAAGCCGC TTCCCTCCAG TCGTGTTCTA CACCCCCAAA 
GAGCTCGGGG GGCTGGGCAT GTTGTCCATG GGCCACGTGC TCATCCCCCA GTCTGATCTC 
AGGTGGTCCA AGCAGACAGA TGTGGGCATC ACCCACTTCC GCTCCGGGAT GAGCCACGAA 
GAAGACCAGC TCATTCCTAA CTTGTACCGT TACATCCAGC CTTGGGAGAG CGAGTTCATT 
GACTCTCAAC GGGTCTGGGC CGAGTATGCG CTCAAGAGGC AGGAAGCCAT CGCTCAGAAC 
AGGCGGCTGA CCTTGGAGGA CCTGGAGGAT TCATGGGATC GAGGCATTCC CCGGATCAAT 
ACCCTGTTCC AAAAGGACCG GCACACACTG GCTTATGACA AGGGCTGGCG TGTCCGCACT 
GACTTCAAGC AATACCAGGT CCTGAAACAG AATCCTTTCT GGTGGACCCA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAATTAC CGCACAGACA TGATCCAGGC CCTGGGTGGT 
GTGGAGGGCA TCCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGT 
CTCTTCTGGG AGAAGGCCAG TGGTTTTGAG GAGTCGATGA AATGGAAGAA GCTGACCAAT 
GCCCAGCGGT CCGGCTTGAA CCAGATCCCC AACCGTCGCT TCACACTGTG GTGGTCGCCA 
ACCATTAACC GCGCCAATGT GTATGTAGGT TTCCAGGTCC AGCTGGACCT TACTGGCATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTCA TCCAGATTTT CCGTGCCCAT 
TTATGGCAGA AGATCCATGA GAGTATTGTC ATGGACTTGT GTCAGGTGTT TGACCAAGAA 
TTGGATGCGC TGGAAATTGA GACGGTGCAG AAGGAGACAA TTCACCCCCG GAAGTCCTAT 
AAGATGAACT CATCTTGTGC AGACATTCTG CTTTTTGCCT CCTACAAATG GAACGTCTCC 
CGGCCGTCTC TGCTGGCTGA CTCCAAGGAC GTGATGGACA GCACCACCAC TCAGAAGTAC 
TGGATCGACA TCCAGCTGCG CTGGGGCGAC TATGACTCCC ACGATATCGA ACGCTACGCT 
CGGGCCAAGT TTCTGGATTA CACTACCGAC AACATGAGTA TCTACCCCTC TCCAACGGGG 
GTGCTCATTG CCATAGACCT GGCATACAAT CTGCACAGCG CCTATGGAAA CTGGTTTCCA 
GGCAGCAAGC CGCTGATCCA GCAAGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCTTG 
TACGTGCTCC GAGAGCGAAT ACGCAAGGGG TTGCAGCTGT ACTCCTCCGA GCCCACAGAG 
CCTTACCTCT CCTCCCAGAA CTATGGCGAG CTCTTCTCCA ACCAGATCAT CTGGTTTGTA 
GATGACACCA ATGTCTACAG AGTCACCATT CACAAGACTT TTGAAGGGAA CTTGACTACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AATCCAAGGA CGGGGCAGCT CTTCCTGAAG 
ATCATCCACA CGTCTGTGTG GGCTGGACAG AAGCGTCTGG GTCAGTTGGC CAAGTGGAAG 
ACAGCCGAGG AAGTGGCTGC CCTGATCCGT TCTCTCCCTG TAGAAGAGCA GCCCAAACAG 
ATTATTGTGA CCAGAAAAGG CATGTTGGAC CCCTTGGAGG TGCACTTACT GGACTTCCCC 
AACATTGTGA TCAAAGGCTC GGAGCTGCAG CTGCCCTTCC AGGCCTGTCT CAAAGTGGAA 
AAATTCGGGG ACCTTATCCT CAAGGCCACC GAGCCCCAAA TGGTTCTCTT CAATCTCTAT 
GACGACTGGC TCAGAACCAT CTCATCCTAC ACGGCCTTTT CCCGTCTGAT CCTGATTCTC 
CGTGCCCTTC ATGTGAACAA TGACCGGGCC AAAGTGATCT TGAAGCCAGA CAAGACCACC 
ATCACAGAGC CGCACCACAT TTGGCCCACG CTGACCGACG AGGAGTGGAT CAAGGTTGAG 
GTGCAGCTCA AGGACTTGAT CCTGGCTGAT TACGGCAAAA AGAACAACGT CAACGTGGCC 
TCACTGACAC AATCTGAGAT CCGGGACATC ATTCTGGGCA TGGAAATCTC CGCCCCTTCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAGCAGTC CCAGCTGACG 
GCAACACAGA CCCGGACTGT CAACAAGCAC GGTGATGAGA TCATCACTTC CACCACCAGC 
AACTACGAGA CACAAACTTT TTCCTCGAAG ACCGAGTGGA GGGTCAGGGC CATCTCTGCA 
GCCAACCTGC ATCTGCGGAC CAACCACATC TACGTGTCAT CTGATGACAT CAAAGAGACA 
GGCTACACTT ACATCCTTCC CAAGAATGTC CTCAAGAAAT TCATCTGCAT TTCTGATCTT 
CGGGCCCAGA TTGCAGGCTA CCTCTATGGA GTGAGCCCCC CGGACAACCC TCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTGCCTCAG TGGGGCACCC ACCAGACGGT GCACCTGCCG 
GGCCAGCTTC CCCAACACGA GTACCTCAAG GAGATGGAGC CCCTCGGCTG GATCCACACA 
CAGCCCAACG AGTCCCCCCA GCTCTCGCCC CAGGATGTCA CCACCCATGC CAAGGTCATG 
GCCGACAACC CCTCATGGGA CGGGGAGAAG ACAATCATCA TCACCTGCAG CTTTACCCCG 
GGCTCTTGCA CCCTGACGGC CTACAAGCTG ACGCCCAGTG GTTACGAGTG GGGTCGGCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACTTGCCTT CCCACTATGA GCGTGTCCAG 
ATGCTGCTCT CTGACCGCTT CCTTGGCTTC TTCATGGTCC CCGCTCAGGC GTCCTGGAAC 
TACAATTTCA TGGGTGTTCG GCACGACCCC AACATGAAAT ACGAGCTGCA GCTGGCCAAC 
CCCAAAGAGT TCTATCACGA GGTGCACCGG CCCTCCCACT TCCTGAACTT CGCGCTGCTG 
CAGGAGGGGG AGGTGTACTC GGCCGACCGA AAGGACCTGT ACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF8 ( XM_003770117.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003770165.1 Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), transcript variant X2, mRNA.

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CloneID	OSa132365
Clone ID Related Accession (Same CDS sequence)	XM_012549867.1 , XM_023503711.1 , XM_003770117.1
Accession Version	XM_003770117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003839017.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG TGTTTCCCTA CAGAGGGCCG GGGACCCCCG TGCCCGGCCC TCTGGCACCG 
CTGCCCGACT ACATGTCAGA GGAGAAGCTT CAGGAAAAAG CCCGAAAATG GCAGCAGTTG 
CAGGCAAAAC GCTATGCAGA GAAGCGGAAA TTTGGATTTG TAGACGCGCA AAAGGAAGAC 
ATGCCTCCAG AGCACGTCAG GAAGATCATC CGGGACCATG GGGACATGAC GAACCGCAAG 
TTCCGTCATG ACAAGCGAGT CTACCTGGGG GCCCTGAAGT ACATGCCGCA TGCTGTCCTC 
AAGCTCTTGG AAAACATGCC CATGCCCTGG GAGCAGATCC GGGATGTGCC TGTTCTGTAC 
CACATCACTG GTGCGATTTC CTTTGTTAAT GAGATTCCTT GGGTCATCGA GCCTGTCTAC 
ATCTCTCAGT GGGGGTCCAT GTGGATCATG ATGCGTCGAG AGAAGAGGGA CAGGCGGCAC 
TTCAAGCGTA TGCGTTTTCC CCCATTTGAT GATGAGGAGC CGCCCCTTGA CTATGCTGAC 
AACATCCTCG ATGTGGAGCC TTTGGAAGCC ATTCAGCTGG AACTGGACCC TGAGGAGGAT 
GCCCCTGTGC TAGACTGGTT TTATGACCAC CAGCCCTTAA GAGACAGTAG GAAGTACGTG 
AATGGCTCTA CTTATCAGCG CTGGCAGTTC ACCCTGCCCA TGATGTCCAC TCTGTACCGC 
CTGGCCAATC AGCTCCTAAC TGATCTTGTG GATGACAATT ACTTCTATCT GTTTGACCTA 
AAGGCGTTTT TTACCTCTAA GGCCCTCAAC ATGGCTATCC CAGGGGGCCC TAAATTTGAA 
CCGCTGGTCC GAGATATCAA TCTTCAGGAT GAAGACTGGA ATGAATTTAA TGATATCAAC 
AAGATCATCA TCAGGCAGCC CATCAGAACC GAATATAAGA TTGCTTTTCC ATACTTGTAC 
AACAACCTTC CACACCACGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACAGAGG ACCCTGATCT TCCAGCCTTC TACTTTGATC CTTTGATCAA TCCTATCTCC 
CACAGACATT CTGTCAAGAG TCAAGAACCT TTGCCAGATG ATGACGAGGA GTTTGAGCTG 
CCCGAGTTTG TGGAACCTTT CCTCAAGGAC ACGCCCCTTT ACACAGATAA TACTGCCAAT 
GGCATCGCCC TTCTGTGGGC ACCTAGGCCC TTCAACCTTC GCTCTGGTCG CACCCGCCGT 
GCCCTGGACA TACCCCTGGT TAAAAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAGGTGA GGGTCTCCTA CCAAAAGCTA CTCAAATACT ACGTGCTGAA TGCCCTCAAG 
CACCGACCCC CCAAGGCCCA GAAAAAGAGG TATCTCTTCC GATCCTTTAA GGCCACCAAG 
TTCTTCCAGT CTACCAAGCT GGACTGGGTG GAGGTAGGGC TGCAGGTCTG CCGCCAGGGC 
TACAATATGC TCAACCTCCT CATCCACCGG AAGAATCTCA ACTACTTGCA TCTGGACTAT 
AACTTCAACC TAAAGCCTGT CAAGACTCTC ACCACCAAGG AAAGAAAGAA GTCTCGCTTT 
GGAAATGCCT TCCACTTGTG TCGGGAGGTG CTGCGGCTGA CCAAGCTCGT AGTGGACAGC 
CACGTTCAGT ACCGCTTGGG CAACGTGGAT GCCTTCCAGC TAGCGGACGG GCTGCAGTAT 
ATATTTGCCC ACGTGGGACA GCTGACAGGA ATGTACCGCT ACAAGTACAA GTTGATGCGG 
CAGATCCGCA TGTGCAAGGA CCTGAAGCAC CTTATCTACT ACCGCTTCAA CACGGGTCCC 
GTGGGGAAGG GCCCAGGCTG TGGTTTCTGG GCTGCTGGTT GGCGAGTCTG GTTGTTTTTC 
ATGCGTGGCA TCACCCCTCT TTTGGAACGG TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGTC ACTCAAAGGG GGTGGCAAAG ACCGTGACCA AGCAGCGAGT GGAGTCCCAC 
TTTGACTTGG AGCTCCGGGC AGCGGTGATG CATGATATCT TGGATATGAT GCCTGAGGGC 
ATCAAACAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TCCCCTGGAA GGTTCCTGGG TTGCCGACCC CCATTGAGAA CATGATCCTG 
CGCTACGTGA AGGCGAAGGC GGATTGGTGG ACCAACACCG CCCATTACAA CCGAGAGCGG 
ATCCGCCGGG GAGCCACCGT CGACAAGACC GTTTGTAAGA AGAACTTGGG TCGGCTCACA 
CGGCTCTATC TGAAGGCTGA ACAGGAACGA CAGCACAACT ACCTAAAGGA TGGTCCCTAC 
ATCACAGCAG AAGAGGCAGT GGCTGTCTAC ACCACCACTG TGCACTGGCT GGAGAGCCGC 
AGATTCTCCC CCATCCCTTT CCCCCCACTC TCCTACAAGC ATGACACCAA GCTACTCATC 
CTGGCCCTGG AGAGGCTCAA GGAGGCCTAC AGTGTGAAGT CCCGTTTGAA CCAATCCCAA 
AGAGAGGAGC TGGGCCTGAT TGAGCAAGCC TATGACAACC CGCACGAGGC TCTGTCTCGT 
ATCAAGCGTC ACTTACTCAC GCAGAGAGCC TTCAAGGAGG TGGGCATTGA GTTCATGGAC 
CTGTACAGTC ACCTGGTTCC TGTGTACGAC GTAGAACCCC TCGAGAAGAT CACAGACGCC 
TACTTGGACC AGTACCTGTG GTATGAAGCT GACAAGCGCC GGCTCTTCCC ACCCTGGATC 
AAACCTGCTG ACACGGAGCC GCCTCCCCTG CTGGTGTATA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAG GGAGAGTGCA ACGTCATGCT GGAGTCGCGC 
TTTGAGAAGA TGTACGAGAA GATCGACTTG ACGCTCCTGA ACAGGCTCCT GCGCCTCATT 
GTGGACCACA ACATTGCTGA CTATATGACG GCCAAGAACA ACGTGGTCAT CAACTACAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATTCGAGGAC TTCAGTTCGC CTCCTTCATC 
GTGCAGTACT ACGGGCTGGT GATGGACCTT CTGGTATTGG GCCTACACCG GGCCAGCGAG 
ATGGCTGGGC CTCCCCAAAT GCCCAATGAC TTCCTTAGCT TCCAAGACAT CGCCACAGAA 
GCTGCCCACC CCATCCGCCT TTTCTGTAGA TACATCGACC GCATCCACAT TTTCTTCAGG 
TTCACTGCCG ACGAGGCCCG GGACTTGATC CAGCGCTACC TCACGGAACA CCCTGACCCC 
AACAACGAGA ACATCGTGGG CTACAACAAC AAGAAGTGCT GGCCCCGCGA CGCGCGGATG 
CGCCTCATGA AGCATGACGT CAACTTGGGC CGCGCTGTGT TCTGGGACAT CAAAAACCGA 
CTGCCGCGAT CGGTAACAAC GGTGCAGTGG GAGAACAGCT TTGTGTCAGT GTACAGCAAG 
GACAACCCCA ATCTGCTCTT CAACATGTGC GGCTTCGAGT GCCGCATCCT GCCCAAATGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GATGGTGTTT GGAACCTCCA GAATGAGGTC 
ACAAAGGAGC GGACAGCTCA GTGCTTCCTG CGTGTGGATG ATGAGTCTAT GCAGCGCTTC 
CACAACCGTG TGCGTCAGAT CCTGATGGCC TCTGGCTCGA CCACCTTCAC CAAGATTGTG 
AACAAGTGGA ACACGGCTCT CATTGGGCTC ATGACGTACT TCCGTGAGGC TGTGGTGAAC 
ACCCAGGAGC TGCTGGACCT TCTGGTGAAA TGTGAGAACA AGATCCAGAC CCGAATCAAG 
ATCGGGCTCA ACTCCAAGAT GCCAAGCCGC TTCCCTCCAG TCGTGTTCTA CACCCCCAAA 
GAGCTCGGGG GGCTGGGCAT GTTGTCCATG GGCCACGTGC TCATCCCCCA GTCTGATCTC 
AGGTGGTCCA AGCAGACAGA TGTGGGCATC ACCCACTTCC GCTCCGGGAT GAGCCACGAA 
GAAGACCAGC TCATTCCTAA CTTGTACCGT TACATCCAGC CTTGGGAGAG CGAGTTCATT 
GACTCTCAAC GGGTCTGGGC CGAGTATGCG CTCAAGAGGC AGGAAGCCAT CGCTCAGAAC 
AGGCGGCTGA CCTTGGAGGA CCTGGAGGAT TCATGGGATC GAGGCATTCC CCGGATCAAT 
ACCCTGTTCC AAAAGGACCG GCACACACTG GCTTATGACA AGGGCTGGCG TGTCCGCACT 
GACTTCAAGC AATACCAGGT CCTGAAACAG AATCCTTTCT GGTGGACCCA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAATTAC CGCACAGACA TGATCCAGGC CCTGGGTGGT 
GTGGAGGGCA TCCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGT 
CTCTTCTGGG AGAAGGCCAG TGGTTTTGAG GAGTCGATGA AATGGAAGAA GCTGACCAAT 
GCCCAGCGGT CCGGCTTGAA CCAGATCCCC AACCGTCGCT TCACACTGTG GTGGTCGCCA 
ACCATTAACC GCGCCAATGT GTATGTAGGT TTCCAGGTCC AGCTGGACCT TACTGGCATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTCA TCCAGATTTT CCGTGCCCAT 
TTATGGCAGA AGATCCATGA GAGTATTGTC ATGGACTTGT GTCAGGTGTT TGACCAAGAA 
TTGGATGCGC TGGAAATTGA GACGGTGCAG AAGGAGACAA TTCACCCCCG GAAGTCCTAT 
AAGATGAACT CATCTTGTGC AGACATTCTG CTTTTTGCCT CCTACAAATG GAACGTCTCC 
CGGCCGTCTC TGCTGGCTGA CTCCAAGGAC GTGATGGACA GCACCACCAC TCAGAAGTAC 
TGGATCGACA TCCAGCTGCG CTGGGGCGAC TATGACTCCC ACGATATCGA ACGCTACGCT 
CGGGCCAAGT TTCTGGATTA CACTACCGAC AACATGAGTA TCTACCCCTC TCCAACGGGG 
GTGCTCATTG CCATAGACCT GGCATACAAT CTGCACAGCG CCTATGGAAA CTGGTTTCCA 
GGCAGCAAGC CGCTGATCCA GCAAGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCTTG 
TACGTGCTCC GAGAGCGAAT ACGCAAGGGG TTGCAGCTGT ACTCCTCCGA GCCCACAGAG 
CCTTACCTCT CCTCCCAGAA CTATGGCGAG CTCTTCTCCA ACCAGATCAT CTGGTTTGTA 
GATGACACCA ATGTCTACAG AGTCACCATT CACAAGACTT TTGAAGGGAA CTTGACTACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AATCCAAGGA CGGGGCAGCT CTTCCTGAAG 
ATCATCCACA CGTCTGTGTG GGCTGGACAG AAGCGTCTGG GTCAGTTGGC CAAGTGGAAG 
ACAGCCGAGG AAGTGGCTGC CCTGATCCGT TCTCTCCCTG TAGAAGAGCA GCCCAAACAG 
ATTATTGTGA CCAGAAAAGG CATGTTGGAC CCCTTGGAGG TGCACTTACT GGACTTCCCC 
AACATTGTGA TCAAAGGCTC GGAGCTGCAG CTGCCCTTCC AGGCCTGTCT CAAAGTGGAA 
AAATTCGGGG ACCTTATCCT CAAGGCCACC GAGCCCCAAA TGGTTCTCTT CAATCTCTAT 
GACGACTGGC TCAGAACCAT CTCATCCTAC ACGGCCTTTT CCCGTCTGAT CCTGATTCTC 
CGTGCCCTTC ATGTGAACAA TGACCGGGCC AAAGTGATCT TGAAGCCAGA CAAGACCACC 
ATCACAGAGC CGCACCACAT TTGGCCCACG CTGACCGACG AGGAGTGGAT CAAGGTTGAG 
GTGCAGCTCA AGGACTTGAT CCTGGCTGAT TACGGCAAAA AGAACAACGT CAACGTGGCC 
TCACTGACAC AATCTGAGAT CCGGGACATC ATTCTGGGCA TGGAAATCTC CGCCCCTTCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAGCAGTC CCAGCTGACG 
GCAACACAGA CCCGGACTGT CAACAAGCAC GGTGATGAGA TCATCACTTC CACCACCAGC 
AACTACGAGA CACAAACTTT TTCCTCGAAG ACCGAGTGGA GGGTCAGGGC CATCTCTGCA 
GCCAACCTGC ATCTGCGGAC CAACCACATC TACGTGTCAT CTGATGACAT CAAAGAGACA 
GGCTACACTT ACATCCTTCC CAAGAATGTC CTCAAGAAAT TCATCTGCAT TTCTGATCTT 
CGGGCCCAGA TTGCAGGCTA CCTCTATGGA GTGAGCCCCC CGGACAACCC TCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTGCCTCAG TGGGGCACCC ACCAGACGGT GCACCTGCCG 
GGCCAGCTTC CCCAACACGA GTACCTCAAG GAGATGGAGC CCCTCGGCTG GATCCACACA 
CAGCCCAACG AGTCCCCCCA GCTCTCGCCC CAGGATGTCA CCACCCATGC CAAGGTCATG 
GCCGACAACC CCTCATGGGA CGGGGAGAAG ACAATCATCA TCACCTGCAG CTTTACCCCG 
GGCTCTTGCA CCCTGACGGC CTACAAGCTG ACGCCCAGTG GTTACGAGTG GGGTCGGCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACTTGCCTT CCCACTATGA GCGTGTCCAG 
ATGCTGCTCT CTGACCGCTT CCTTGGCTTC TTCATGGTCC CCGCTCAGGC GTCCTGGAAC 
TACAATTTCA TGGGTGTTCG GCACGACCCC AACATGAAAT ACGAGCTGCA GCTGGCCAAC 
CCCAAAGAGT TCTATCACGA GGTGCACCGG CCCTCCCACT TCCTGAACTT CGCGCTGCTG 
CAGGAGGGGG AGGTGTACTC GGCCGACCGA AAGGACCTGT ACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003770165.1
CDS	73..7080
Translation	MAGVFPYRGPGTPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLRTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKVMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQASWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADRKDLYA

Target ORF information:

RefSeq Version	XM_003770117.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003770117.1

ORF Insert Sequence:

ATGGCCGGGG TGTTTCCCTA CAGAGGGCCG GGGACCCCCG TGCCCGGCCC TCTGGCACCG 
CTGCCCGACT ACATGTCAGA GGAGAAGCTT CAGGAAAAAG CCCGAAAATG GCAGCAGTTG 
CAGGCAAAAC GCTATGCAGA GAAGCGGAAA TTTGGATTTG TAGACGCGCA AAAGGAAGAC 
ATGCCTCCAG AGCACGTCAG GAAGATCATC CGGGACCATG GGGACATGAC GAACCGCAAG 
TTCCGTCATG ACAAGCGAGT CTACCTGGGG GCCCTGAAGT ACATGCCGCA TGCTGTCCTC 
AAGCTCTTGG AAAACATGCC CATGCCCTGG GAGCAGATCC GGGATGTGCC TGTTCTGTAC 
CACATCACTG GTGCGATTTC CTTTGTTAAT GAGATTCCTT GGGTCATCGA GCCTGTCTAC 
ATCTCTCAGT GGGGGTCCAT GTGGATCATG ATGCGTCGAG AGAAGAGGGA CAGGCGGCAC 
TTCAAGCGTA TGCGTTTTCC CCCATTTGAT GATGAGGAGC CGCCCCTTGA CTATGCTGAC 
AACATCCTCG ATGTGGAGCC TTTGGAAGCC ATTCAGCTGG AACTGGACCC TGAGGAGGAT 
GCCCCTGTGC TAGACTGGTT TTATGACCAC CAGCCCTTAA GAGACAGTAG GAAGTACGTG 
AATGGCTCTA CTTATCAGCG CTGGCAGTTC ACCCTGCCCA TGATGTCCAC TCTGTACCGC 
CTGGCCAATC AGCTCCTAAC TGATCTTGTG GATGACAATT ACTTCTATCT GTTTGACCTA 
AAGGCGTTTT TTACCTCTAA GGCCCTCAAC ATGGCTATCC CAGGGGGCCC TAAATTTGAA 
CCGCTGGTCC GAGATATCAA TCTTCAGGAT GAAGACTGGA ATGAATTTAA TGATATCAAC 
AAGATCATCA TCAGGCAGCC CATCAGAACC GAATATAAGA TTGCTTTTCC ATACTTGTAC 
AACAACCTTC CACACCACGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACAGAGG ACCCTGATCT TCCAGCCTTC TACTTTGATC CTTTGATCAA TCCTATCTCC 
CACAGACATT CTGTCAAGAG TCAAGAACCT TTGCCAGATG ATGACGAGGA GTTTGAGCTG 
CCCGAGTTTG TGGAACCTTT CCTCAAGGAC ACGCCCCTTT ACACAGATAA TACTGCCAAT 
GGCATCGCCC TTCTGTGGGC ACCTAGGCCC TTCAACCTTC GCTCTGGTCG CACCCGCCGT 
GCCCTGGACA TACCCCTGGT TAAAAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAGGTGA GGGTCTCCTA CCAAAAGCTA CTCAAATACT ACGTGCTGAA TGCCCTCAAG 
CACCGACCCC CCAAGGCCCA GAAAAAGAGG TATCTCTTCC GATCCTTTAA GGCCACCAAG 
TTCTTCCAGT CTACCAAGCT GGACTGGGTG GAGGTAGGGC TGCAGGTCTG CCGCCAGGGC 
TACAATATGC TCAACCTCCT CATCCACCGG AAGAATCTCA ACTACTTGCA TCTGGACTAT 
AACTTCAACC TAAAGCCTGT CAAGACTCTC ACCACCAAGG AAAGAAAGAA GTCTCGCTTT 
GGAAATGCCT TCCACTTGTG TCGGGAGGTG CTGCGGCTGA CCAAGCTCGT AGTGGACAGC 
CACGTTCAGT ACCGCTTGGG CAACGTGGAT GCCTTCCAGC TAGCGGACGG GCTGCAGTAT 
ATATTTGCCC ACGTGGGACA GCTGACAGGA ATGTACCGCT ACAAGTACAA GTTGATGCGG 
CAGATCCGCA TGTGCAAGGA CCTGAAGCAC CTTATCTACT ACCGCTTCAA CACGGGTCCC 
GTGGGGAAGG GCCCAGGCTG TGGTTTCTGG GCTGCTGGTT GGCGAGTCTG GTTGTTTTTC 
ATGCGTGGCA TCACCCCTCT TTTGGAACGG TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGTC ACTCAAAGGG GGTGGCAAAG ACCGTGACCA AGCAGCGAGT GGAGTCCCAC 
TTTGACTTGG AGCTCCGGGC AGCGGTGATG CATGATATCT TGGATATGAT GCCTGAGGGC 
ATCAAACAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TCCCCTGGAA GGTTCCTGGG TTGCCGACCC CCATTGAGAA CATGATCCTG 
CGCTACGTGA AGGCGAAGGC GGATTGGTGG ACCAACACCG CCCATTACAA CCGAGAGCGG 
ATCCGCCGGG GAGCCACCGT CGACAAGACC GTTTGTAAGA AGAACTTGGG TCGGCTCACA 
CGGCTCTATC TGAAGGCTGA ACAGGAACGA CAGCACAACT ACCTAAAGGA TGGTCCCTAC 
ATCACAGCAG AAGAGGCAGT GGCTGTCTAC ACCACCACTG TGCACTGGCT GGAGAGCCGC 
AGATTCTCCC CCATCCCTTT CCCCCCACTC TCCTACAAGC ATGACACCAA GCTACTCATC 
CTGGCCCTGG AGAGGCTCAA GGAGGCCTAC AGTGTGAAGT CCCGTTTGAA CCAATCCCAA 
AGAGAGGAGC TGGGCCTGAT TGAGCAAGCC TATGACAACC CGCACGAGGC TCTGTCTCGT 
ATCAAGCGTC ACTTACTCAC GCAGAGAGCC TTCAAGGAGG TGGGCATTGA GTTCATGGAC 
CTGTACAGTC ACCTGGTTCC TGTGTACGAC GTAGAACCCC TCGAGAAGAT CACAGACGCC 
TACTTGGACC AGTACCTGTG GTATGAAGCT GACAAGCGCC GGCTCTTCCC ACCCTGGATC 
AAACCTGCTG ACACGGAGCC GCCTCCCCTG CTGGTGTATA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAG GGAGAGTGCA ACGTCATGCT GGAGTCGCGC 
TTTGAGAAGA TGTACGAGAA GATCGACTTG ACGCTCCTGA ACAGGCTCCT GCGCCTCATT 
GTGGACCACA ACATTGCTGA CTATATGACG GCCAAGAACA ACGTGGTCAT CAACTACAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATTCGAGGAC TTCAGTTCGC CTCCTTCATC 
GTGCAGTACT ACGGGCTGGT GATGGACCTT CTGGTATTGG GCCTACACCG GGCCAGCGAG 
ATGGCTGGGC CTCCCCAAAT GCCCAATGAC TTCCTTAGCT TCCAAGACAT CGCCACAGAA 
GCTGCCCACC CCATCCGCCT TTTCTGTAGA TACATCGACC GCATCCACAT TTTCTTCAGG 
TTCACTGCCG ACGAGGCCCG GGACTTGATC CAGCGCTACC TCACGGAACA CCCTGACCCC 
AACAACGAGA ACATCGTGGG CTACAACAAC AAGAAGTGCT GGCCCCGCGA CGCGCGGATG 
CGCCTCATGA AGCATGACGT CAACTTGGGC CGCGCTGTGT TCTGGGACAT CAAAAACCGA 
CTGCCGCGAT CGGTAACAAC GGTGCAGTGG GAGAACAGCT TTGTGTCAGT GTACAGCAAG 
GACAACCCCA ATCTGCTCTT CAACATGTGC GGCTTCGAGT GCCGCATCCT GCCCAAATGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GATGGTGTTT GGAACCTCCA GAATGAGGTC 
ACAAAGGAGC GGACAGCTCA GTGCTTCCTG CGTGTGGATG ATGAGTCTAT GCAGCGCTTC 
CACAACCGTG TGCGTCAGAT CCTGATGGCC TCTGGCTCGA CCACCTTCAC CAAGATTGTG 
AACAAGTGGA ACACGGCTCT CATTGGGCTC ATGACGTACT TCCGTGAGGC TGTGGTGAAC 
ACCCAGGAGC TGCTGGACCT TCTGGTGAAA TGTGAGAACA AGATCCAGAC CCGAATCAAG 
ATCGGGCTCA ACTCCAAGAT GCCAAGCCGC TTCCCTCCAG TCGTGTTCTA CACCCCCAAA 
GAGCTCGGGG GGCTGGGCAT GTTGTCCATG GGCCACGTGC TCATCCCCCA GTCTGATCTC 
AGGTGGTCCA AGCAGACAGA TGTGGGCATC ACCCACTTCC GCTCCGGGAT GAGCCACGAA 
GAAGACCAGC TCATTCCTAA CTTGTACCGT TACATCCAGC CTTGGGAGAG CGAGTTCATT 
GACTCTCAAC GGGTCTGGGC CGAGTATGCG CTCAAGAGGC AGGAAGCCAT CGCTCAGAAC 
AGGCGGCTGA CCTTGGAGGA CCTGGAGGAT TCATGGGATC GAGGCATTCC CCGGATCAAT 
ACCCTGTTCC AAAAGGACCG GCACACACTG GCTTATGACA AGGGCTGGCG TGTCCGCACT 
GACTTCAAGC AATACCAGGT CCTGAAACAG AATCCTTTCT GGTGGACCCA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAATTAC CGCACAGACA TGATCCAGGC CCTGGGTGGT 
GTGGAGGGCA TCCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGT 
CTCTTCTGGG AGAAGGCCAG TGGTTTTGAG GAGTCGATGA AATGGAAGAA GCTGACCAAT 
GCCCAGCGGT CCGGCTTGAA CCAGATCCCC AACCGTCGCT TCACACTGTG GTGGTCGCCA 
ACCATTAACC GCGCCAATGT GTATGTAGGT TTCCAGGTCC AGCTGGACCT TACTGGCATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTCA TCCAGATTTT CCGTGCCCAT 
TTATGGCAGA AGATCCATGA GAGTATTGTC ATGGACTTGT GTCAGGTGTT TGACCAAGAA 
TTGGATGCGC TGGAAATTGA GACGGTGCAG AAGGAGACAA TTCACCCCCG GAAGTCCTAT 
AAGATGAACT CATCTTGTGC AGACATTCTG CTTTTTGCCT CCTACAAATG GAACGTCTCC 
CGGCCGTCTC TGCTGGCTGA CTCCAAGGAC GTGATGGACA GCACCACCAC TCAGAAGTAC 
TGGATCGACA TCCAGCTGCG CTGGGGCGAC TATGACTCCC ACGATATCGA ACGCTACGCT 
CGGGCCAAGT TTCTGGATTA CACTACCGAC AACATGAGTA TCTACCCCTC TCCAACGGGG 
GTGCTCATTG CCATAGACCT GGCATACAAT CTGCACAGCG CCTATGGAAA CTGGTTTCCA 
GGCAGCAAGC CGCTGATCCA GCAAGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCTTG 
TACGTGCTCC GAGAGCGAAT ACGCAAGGGG TTGCAGCTGT ACTCCTCCGA GCCCACAGAG 
CCTTACCTCT CCTCCCAGAA CTATGGCGAG CTCTTCTCCA ACCAGATCAT CTGGTTTGTA 
GATGACACCA ATGTCTACAG AGTCACCATT CACAAGACTT TTGAAGGGAA CTTGACTACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AATCCAAGGA CGGGGCAGCT CTTCCTGAAG 
ATCATCCACA CGTCTGTGTG GGCTGGACAG AAGCGTCTGG GTCAGTTGGC CAAGTGGAAG 
ACAGCCGAGG AAGTGGCTGC CCTGATCCGT TCTCTCCCTG TAGAAGAGCA GCCCAAACAG 
ATTATTGTGA CCAGAAAAGG CATGTTGGAC CCCTTGGAGG TGCACTTACT GGACTTCCCC 
AACATTGTGA TCAAAGGCTC GGAGCTGCAG CTGCCCTTCC AGGCCTGTCT CAAAGTGGAA 
AAATTCGGGG ACCTTATCCT CAAGGCCACC GAGCCCCAAA TGGTTCTCTT CAATCTCTAT 
GACGACTGGC TCAGAACCAT CTCATCCTAC ACGGCCTTTT CCCGTCTGAT CCTGATTCTC 
CGTGCCCTTC ATGTGAACAA TGACCGGGCC AAAGTGATCT TGAAGCCAGA CAAGACCACC 
ATCACAGAGC CGCACCACAT TTGGCCCACG CTGACCGACG AGGAGTGGAT CAAGGTTGAG 
GTGCAGCTCA AGGACTTGAT CCTGGCTGAT TACGGCAAAA AGAACAACGT CAACGTGGCC 
TCACTGACAC AATCTGAGAT CCGGGACATC ATTCTGGGCA TGGAAATCTC CGCCCCTTCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAGCAGTC CCAGCTGACG 
GCAACACAGA CCCGGACTGT CAACAAGCAC GGTGATGAGA TCATCACTTC CACCACCAGC 
AACTACGAGA CACAAACTTT TTCCTCGAAG ACCGAGTGGA GGGTCAGGGC CATCTCTGCA 
GCCAACCTGC ATCTGCGGAC CAACCACATC TACGTGTCAT CTGATGACAT CAAAGAGACA 
GGCTACACTT ACATCCTTCC CAAGAATGTC CTCAAGAAAT TCATCTGCAT TTCTGATCTT 
CGGGCCCAGA TTGCAGGCTA CCTCTATGGA GTGAGCCCCC CGGACAACCC TCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTGCCTCAG TGGGGCACCC ACCAGACGGT GCACCTGCCG 
GGCCAGCTTC CCCAACACGA GTACCTCAAG GAGATGGAGC CCCTCGGCTG GATCCACACA 
CAGCCCAACG AGTCCCCCCA GCTCTCGCCC CAGGATGTCA CCACCCATGC CAAGGTCATG 
GCCGACAACC CCTCATGGGA CGGGGAGAAG ACAATCATCA TCACCTGCAG CTTTACCCCG 
GGCTCTTGCA CCCTGACGGC CTACAAGCTG ACGCCCAGTG GTTACGAGTG GGGTCGGCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACTTGCCTT CCCACTATGA GCGTGTCCAG 
ATGCTGCTCT CTGACCGCTT CCTTGGCTTC TTCATGGTCC CCGCTCAGGC GTCCTGGAAC 
TACAATTTCA TGGGTGTTCG GCACGACCCC AACATGAAAT ACGAGCTGCA GCTGGCCAAC 
CCCAAAGAGT TCTATCACGA GGTGCACCGG CCCTCCCACT TCCTGAACTT CGCGCTGCTG 
CAGGAGGGGG AGGTGTACTC GGCCGACCGA AAGGACCTGT ACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF8 ( XM_031967785.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823645.1 Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), transcript variant X2, mRNA.

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CloneID	OSa234804
Clone ID Related Accession (Same CDS sequence)	XM_031967785.1 , XM_023503711.2 , XM_031967786.1
Accession Version	XM_031967785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG TGTTTCCCTA CAGAGGGCCG GGGACCCCCG TGCCCGGCCC TCTGGCACCG 
CTGCCCGACT ACATGTCAGA GGAGAAGCTT CAGGAAAAAG CCCGAAAATG GCAGCAGTTG 
CAGGCAAAAC GCTATGCAGA GAAGCGGAAA TTTGGATTTG TAGACGCGCA AAAGGAAGAC 
ATGCCTCCAG AGCACGTCAG GAAGATCATC CGGGACCATG GGGACATGAC GAACCGCAAG 
TTCCGTCATG ACAAGCGAGT CTACCTGGGG GCCCTGAAGT ACATGCCGCA TGCTGTCCTC 
AAGCTCTTGG AAAACATGCC CATGCCCTGG GAGCAGATCC GGGATGTGCC TGTTCTGTAC 
CACATCACTG GTGCGATTTC CTTTGTTAAT GAGATTCCTT GGGTCATCGA GCCTGTCTAC 
ATCTCTCAGT GGGGGTCCAT GTGGATCATG ATGCGTCGAG AGAAGAGGGA CAGGCGGCAC 
TTCAAGCGTA TGCGTTTTCC CCCATTTGAT GATGAGGAGC CGCCCCTTGA CTATGCTGAC 
AACATCCTCG ATGTGGAGCC TTTGGAAGCC ATTCAGCTGG AACTGGACCC TGAGGAGGAT 
GCCCCTGTGC TAGACTGGTT TTATGACCAC CAGCCCTTAA GAGACAGTAG GAAGTACGTG 
AATGGCTCTA CTTATCAGCG CTGGCAGTTC ACCCTGCCCA TGATGTCCAC TCTGTACCGC 
CTGGCCAATC AGCTCCTAAC TGATCTTGTG GATGACAATT ACTTCTATCT GTTTGACCTA 
AAGGCGTTTT TTACCTCTAA GGCCCTCAAC ATGGCTATCC CAGGGGGCCC TAAATTTGAA 
CCGCTGGTCC GAGATATCAA TCTTCAGGAT GAAGACTGGA ATGAATTTAA TGATATCAAC 
AAGATCATCA TCAGGCAGCC CATCAGAACC GAATATAAGA TTGCTTTTCC ATACTTGTAC 
AACAACCTTC CACACCACGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACAGAGG ACCCTGATCT TCCAGCCTTC TACTTTGATC CTTTGATCAA TCCTATCTCC 
CACAGACATT CTGTCAAGAG TCAAGAACCT TTGCCAGATG ATGACGAGGA GTTTGAGCTG 
CCCGAGTTTG TGGAACCTTT CCTCAAGGAC ACGCCCCTTT ACACAGATAA TACTGCCAAT 
GGCATCGCCC TTCTGTGGGC ACCTAGGCCC TTCAACCTTC GCTCTGGTCG CACCCGCCGT 
GCCCTGGACA TACCCCTGGT TAAAAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAGGTGA GGGTCTCCTA CCAAAAGCTA CTCAAATACT ACGTGCTGAA TGCCCTCAAG 
CACCGACCCC CCAAGGCCCA GAAAAAGAGG TATCTCTTCC GATCCTTTAA GGCCACCAAG 
TTCTTCCAGT CTACCAAGCT GGACTGGGTG GAGGTAGGGC TGCAGGTCTG CCGCCAGGGC 
TACAATATGC TCAACCTCCT CATCCACCGG AAGAATCTCA ACTACTTGCA TCTGGACTAT 
AACTTCAACC TAAAGCCTGT CAAGACTCTC ACCACCAAGG AAAGAAAGAA GTCTCGCTTT 
GGAAATGCCT TCCACTTGTG TCGGGAGGTG CTGCGGCTGA CCAAGCTCGT AGTGGACAGC 
CACGTTCAGT ACCGCTTGGG CAACGTGGAT GCCTTCCAGC TAGCGGACGG GCTGCAGTAT 
ATATTTGCCC ACGTGGGACA GCTGACAGGA ATGTACCGCT ACAAGTACAA GTTGATGCGG 
CAGATCCGCA TGTGCAAGGA CCTGAAGCAC CTTATCTACT ACCGCTTCAA CACGGGTCCC 
GTGGGGAAGG GCCCAGGCTG TGGTTTCTGG GCTGCTGGTT GGCGAGTCTG GTTGTTTTTC 
ATGCGTGGCA TCACCCCTCT TTTGGAACGG TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGTC ACTCAAAGGG GGTGGCAAAG ACCGTGACCA AGCAGCGAGT GGAGTCCCAC 
TTTGACTTGG AGCTCCGGGC AGCGGTGATG CATGATATCT TGGATATGAT GCCTGAGGGC 
ATCAAACAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TCCCCTGGAA GGTTCCTGGG TTGCCGACCC CCATTGAGAA CATGATCCTG 
CGCTACGTGA AGGCGAAGGC GGATTGGTGG ACCAACACCG CCCATTACAA CCGAGAGCGG 
ATCCGCCGGG GAGCCACCGT CGACAAGACC GTTTGTAAGA AGAACTTGGG TCGGCTCACA 
CGGCTCTATC TGAAGGCTGA ACAGGAACGA CAGCACAACT ACCTAAAGGA TGGTCCCTAC 
ATCACAGCAG AAGAGGCAGT GGCTGTCTAC ACCACCACTG TGCACTGGCT GGAGAGCCGC 
AGATTCTCCC CCATCCCTTT CCCCCCACTC TCCTACAAGC ATGACACCAA GCTACTCATC 
CTGGCCCTGG AGAGGCTCAA GGAGGCCTAC AGTGTGAAGT CCCGTTTGAA CCAATCCCAA 
AGAGAGGAGC TGGGCCTGAT TGAGCAAGCC TATGACAACC CGCACGAGGC TCTGTCTCGT 
ATCAAGCGTC ACTTACTCAC GCAGAGAGCC TTCAAGGAGG TGGGCATTGA GTTCATGGAC 
CTGTACAGTC ACCTGGTTCC TGTGTACGAC GTAGAACCCC TCGAGAAGAT CACAGACGCC 
TACTTGGACC AGTACCTGTG GTATGAAGCT GACAAGCGCC GGCTCTTCCC ACCCTGGATC 
AAACCTGCTG ACACGGAGCC GCCTCCCCTG CTGGTGTATA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAG GGAGAGTGCA ACGTCATGCT GGAGTCGCGC 
TTTGAGAAGA TGTACGAGAA GATCGACTTG ACGCTCCTGA ACAGGCTCCT GCGCCTCATT 
GTGGACCACA ACATTGCTGA CTATATGACG GCCAAGAACA ACGTGGTCAT CAACTACAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATTCGAGGAC TTCAGTTCGC CTCCTTCATC 
GTGCAGTACT ACGGGCTGGT GATGGACCTT CTGGTATTGG GCCTACACCG GGCCAGCGAG 
ATGGCTGGGC CTCCCCAAAT GCCCAATGAC TTCCTTAGCT TCCAAGACAT CGCCACAGAA 
GCTGCCCACC CCATCCGCCT TTTCTGTAGA TACATCGACC GCATCCACAT TTTCTTCAGG 
TTCACTGCCG ACGAGGCCCG GGACTTGATC CAGCGCTACC TCACGGAACA CCCTGACCCC 
AACAACGAGA ACATCGTGGG CTACAACAAC AAGAAGTGCT GGCCCCGCGA CGCGCGGATG 
CGCCTCATGA AGCATGACGT CAACTTGGGC CGCGCTGTGT TCTGGGACAT CAAAAACCGA 
CTGCCGCGAT CGGTAACAAC GGTGCAGTGG GAGAACAGCT TTGTGTCAGT GTACAGCAAG 
GACAACCCCA ATCTGCTCTT CAACATGTGC GGCTTCGAGT GCCGCATCCT GCCCAAATGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GATGGTGTTT GGAACCTCCA GAATGAGGTC 
ACAAAGGAGC GGACAGCTCA GTGCTTCCTG CGTGTGGATG ATGAGTCTAT GCAGCGCTTC 
CACAACCGTG TGCGTCAGAT CCTGATGGCC TCTGGCTCGA CCACCTTCAC CAAGATTGTG 
AACAAGTGGA ACACGGCTCT CATTGGGCTC ATGACGTACT TCCGTGAGGC TGTGGTGAAC 
ACCCAGGAGC TGCTGGACCT TCTGGTGAAA TGTGAGAACA AGATCCAGAC CCGAATCAAG 
ATCGGGCTCA ACTCCAAGAT GCCAAGCCGC TTCCCTCCAG TCGTGTTCTA CACCCCCAAA 
GAGCTCGGGG GGCTGGGCAT GTTGTCCATG GGCCACGTGC TCATCCCCCA GTCTGATCTC 
AGGTGGTCCA AGCAGACAGA TGTGGGCATC ACCCACTTCC GCTCCGGGAT GAGCCACGAA 
GAAGACCAGC TCATTCCTAA CTTGTACCGT TACATCCAGC CTTGGGAGAG CGAGTTCATT 
GACTCTCAAC GGGTCTGGGC CGAGTATGCG CTCAAGAGGC AGGAAGCCAT CGCTCAGAAC 
AGGCGGCTGA CCTTGGAGGA CCTGGAGGAT TCATGGGATC GAGGCATTCC CCGGATCAAT 
ACCCTGTTCC AAAAGGACCG GCACACACTG GCTTATGACA AGGGCTGGCG TGTCCGCACT 
GACTTCAAGC AATACCAGGT CCTGAAACAG AATCCTTTCT GGTGGACCCA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAATTAC CGCACAGACA TGATCCAGGC CCTGGGTGGT 
GTGGAGGGCA TCCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGT 
CTCTTCTGGG AGAAGGCCAG TGGTTTTGAG GAGTCGATGA AATGGAAGAA GCTGACCAAT 
GCCCAGCGGT CCGGCTTGAA CCAGATCCCC AACCGTCGCT TCACACTGTG GTGGTCGCCA 
ACCATTAACC GCGCCAATGT GTATGTAGGT TTCCAGGTCC AGCTGGACCT TACTGGCATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTCA TCCAGATTTT CCGTGCCCAT 
TTATGGCAGA AGATCCATGA GAGTATTGTC ATGGACTTGT GTCAGGTGTT TGACCAAGAA 
TTGGATGCGC TGGAAATTGA GACGGTGCAG AAGGAGACAA TTCACCCCCG GAAGTCCTAT 
AAGATGAACT CATCTTGTGC AGACATTCTG CTTTTTGCCT CCTACAAATG GAACGTCTCC 
CGGCCGTCTC TGCTGGCTGA CTCCAAGGAC GTGATGGACA GCACCACCAC TCAGAAGTAC 
TGGATCGACA TCCAGCTGCG CTGGGGCGAC TATGACTCCC ACGATATCGA ACGCTACGCT 
CGGGCCAAGT TTCTGGATTA CACTACCGAC AACATGAGTA TCTACCCCTC TCCAACGGGG 
GTGCTCATTG CCATAGACCT GGCATACAAT CTGCACAGCG CCTATGGAAA CTGGTTTCCA 
GGCAGCAAGC CGCTGATCCA GCAAGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCTTG 
TACGTGCTCC GAGAGCGAAT ACGCAAGGGG TTGCAGCTGT ACTCCTCCGA GCCCACAGAG 
CCTTACCTCT CCTCCCAGAA CTATGGCGAG CTCTTCTCCA ACCAGATCAT CTGGTTTGTA 
GATGACACCA ATGTCTACAG AGTCACCATT CACAAGACTT TTGAAGGGAA CTTGACTACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AATCCAAGGA CGGGGCAGCT CTTCCTGAAG 
ATCATCCACA CGTCTGTGTG GGCTGGACAG AAGCGTCTGG GTCAGTTGGC CAAGTGGAAG 
ACAGCCGAGG AAGTGGCTGC CCTGATCCGT TCTCTCCCTG TAGAAGAGCA GCCCAAACAG 
ATTATTGTGA CCAGAAAAGG CATGTTGGAC CCCTTGGAGG TGCACTTACT GGACTTCCCC 
AACATTGTGA TCAAAGGCTC GGAGCTGCAG CTGCCCTTCC AGGCCTGTCT CAAAGTGGAA 
AAATTCGGGG ACCTTATCCT CAAGGCCACC GAGCCCCAAA TGGTTCTCTT CAATCTCTAT 
GACGACTGGC TCAGAACCAT CTCATCCTAC ACGGCCTTTT CCCGTCTGAT CCTGATTCTC 
CGTGCCCTTC ATGTGAACAA TGACCGGGCC AAAGTGATCT TGAAGCCAGA CAAGACCACC 
ATCACAGAGC CGCACCACAT TTGGCCCACG CTGACCGACG AGGAGTGGAT CAAGGTTGAG 
GTGCAGCTCA AGGACTTGAT CCTGGCTGAT TACGGCAAAA AGAACAACGT CAACGTGGCC 
TCACTGACAC AATCTGAGAT CCGGGACATC ATTCTGGGCA TGGAAATCTC CGCCCCTTCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAGCAGTC CCAGCTGACG 
GCAACACAGA CCCGGACTGT CAACAAGCAC GGTGATGAGA TCATCACTTC CACCACCAGC 
AACTACGAGA CACAAACTTT TTCCTCGAAG ACCGAGTGGA GGGTCAGGGC CATCTCTGCA 
GCCAACCTGC ATCTGCGGAC CAACCACATC TACGTGTCAT CTGATGACAT CAAAGAGACA 
GGCTACACTT ACATCCTTCC CAAGAATGTC CTCAAGAAAT TCATCTGCAT TTCTGATCTT 
CGGGCCCAGA TTGCAGGCTA CCTCTATGGA GTGAGCCCCC CGGACAACCC TCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTGCCTCAG TGGGGCACCC ACCAGACGGT GCACCTGCCG 
GGCCAGCTTC CCCAACACGA GTACCTCAAG GAGATGGAGC CCCTCGGCTG GATCCACACA 
CAGCCCAACG AGTCCCCCCA GCTCTCGCCC CAGGATGTCA CCACCCATGC CAAGGTCATG 
GCCGACAACC CCTCATGGGA CGGGGAGAAG ACAATCATCA TCACCTGCAG CTTTACCCCG 
GGCTCTTGCA CCCTGACGGC CTACAAGCTG ACGCCCAGTG GTTACGAGTG GGGTCGGCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACTTGCCTT CCCACTATGA GCGTGTCCAG 
ATGCTGCTCT CTGACCGCTT CCTTGGCTTC TTCATGGTCC CCGCTCAGGC GTCCTGGAAC 
TACAATTTCA TGGGTGTTCG GCACGACCCC AACATGAAAT ACGAGCTGCA GCTGGCCAAC 
CCCAAAGAGT TCTATCACGA GGTGCACCGG CCCTCCCACT TCCTGAACTT CGCGCTGCTG 
CAGGAGGGGG AGGTGTACTC GGCCGACCGA GAGGACCTGT ACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823645.1
CDS	82..7089
Translation	MAGVFPYRGPGTPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLRTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKVMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQASWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_031967785.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967785.1

ORF Insert Sequence:

ATGGCCGGGG TGTTTCCCTA CAGAGGGCCG GGGACCCCCG TGCCCGGCCC TCTGGCACCG 
CTGCCCGACT ACATGTCAGA GGAGAAGCTT CAGGAAAAAG CCCGAAAATG GCAGCAGTTG 
CAGGCAAAAC GCTATGCAGA GAAGCGGAAA TTTGGATTTG TAGACGCGCA AAAGGAAGAC 
ATGCCTCCAG AGCACGTCAG GAAGATCATC CGGGACCATG GGGACATGAC GAACCGCAAG 
TTCCGTCATG ACAAGCGAGT CTACCTGGGG GCCCTGAAGT ACATGCCGCA TGCTGTCCTC 
AAGCTCTTGG AAAACATGCC CATGCCCTGG GAGCAGATCC GGGATGTGCC TGTTCTGTAC 
CACATCACTG GTGCGATTTC CTTTGTTAAT GAGATTCCTT GGGTCATCGA GCCTGTCTAC 
ATCTCTCAGT GGGGGTCCAT GTGGATCATG ATGCGTCGAG AGAAGAGGGA CAGGCGGCAC 
TTCAAGCGTA TGCGTTTTCC CCCATTTGAT GATGAGGAGC CGCCCCTTGA CTATGCTGAC 
AACATCCTCG ATGTGGAGCC TTTGGAAGCC ATTCAGCTGG AACTGGACCC TGAGGAGGAT 
GCCCCTGTGC TAGACTGGTT TTATGACCAC CAGCCCTTAA GAGACAGTAG GAAGTACGTG 
AATGGCTCTA CTTATCAGCG CTGGCAGTTC ACCCTGCCCA TGATGTCCAC TCTGTACCGC 
CTGGCCAATC AGCTCCTAAC TGATCTTGTG GATGACAATT ACTTCTATCT GTTTGACCTA 
AAGGCGTTTT TTACCTCTAA GGCCCTCAAC ATGGCTATCC CAGGGGGCCC TAAATTTGAA 
CCGCTGGTCC GAGATATCAA TCTTCAGGAT GAAGACTGGA ATGAATTTAA TGATATCAAC 
AAGATCATCA TCAGGCAGCC CATCAGAACC GAATATAAGA TTGCTTTTCC ATACTTGTAC 
AACAACCTTC CACACCACGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACAGAGG ACCCTGATCT TCCAGCCTTC TACTTTGATC CTTTGATCAA TCCTATCTCC 
CACAGACATT CTGTCAAGAG TCAAGAACCT TTGCCAGATG ATGACGAGGA GTTTGAGCTG 
CCCGAGTTTG TGGAACCTTT CCTCAAGGAC ACGCCCCTTT ACACAGATAA TACTGCCAAT 
GGCATCGCCC TTCTGTGGGC ACCTAGGCCC TTCAACCTTC GCTCTGGTCG CACCCGCCGT 
GCCCTGGACA TACCCCTGGT TAAAAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAGGTGA GGGTCTCCTA CCAAAAGCTA CTCAAATACT ACGTGCTGAA TGCCCTCAAG 
CACCGACCCC CCAAGGCCCA GAAAAAGAGG TATCTCTTCC GATCCTTTAA GGCCACCAAG 
TTCTTCCAGT CTACCAAGCT GGACTGGGTG GAGGTAGGGC TGCAGGTCTG CCGCCAGGGC 
TACAATATGC TCAACCTCCT CATCCACCGG AAGAATCTCA ACTACTTGCA TCTGGACTAT 
AACTTCAACC TAAAGCCTGT CAAGACTCTC ACCACCAAGG AAAGAAAGAA GTCTCGCTTT 
GGAAATGCCT TCCACTTGTG TCGGGAGGTG CTGCGGCTGA CCAAGCTCGT AGTGGACAGC 
CACGTTCAGT ACCGCTTGGG CAACGTGGAT GCCTTCCAGC TAGCGGACGG GCTGCAGTAT 
ATATTTGCCC ACGTGGGACA GCTGACAGGA ATGTACCGCT ACAAGTACAA GTTGATGCGG 
CAGATCCGCA TGTGCAAGGA CCTGAAGCAC CTTATCTACT ACCGCTTCAA CACGGGTCCC 
GTGGGGAAGG GCCCAGGCTG TGGTTTCTGG GCTGCTGGTT GGCGAGTCTG GTTGTTTTTC 
ATGCGTGGCA TCACCCCTCT TTTGGAACGG TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGTC ACTCAAAGGG GGTGGCAAAG ACCGTGACCA AGCAGCGAGT GGAGTCCCAC 
TTTGACTTGG AGCTCCGGGC AGCGGTGATG CATGATATCT TGGATATGAT GCCTGAGGGC 
ATCAAACAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TCCCCTGGAA GGTTCCTGGG TTGCCGACCC CCATTGAGAA CATGATCCTG 
CGCTACGTGA AGGCGAAGGC GGATTGGTGG ACCAACACCG CCCATTACAA CCGAGAGCGG 
ATCCGCCGGG GAGCCACCGT CGACAAGACC GTTTGTAAGA AGAACTTGGG TCGGCTCACA 
CGGCTCTATC TGAAGGCTGA ACAGGAACGA CAGCACAACT ACCTAAAGGA TGGTCCCTAC 
ATCACAGCAG AAGAGGCAGT GGCTGTCTAC ACCACCACTG TGCACTGGCT GGAGAGCCGC 
AGATTCTCCC CCATCCCTTT CCCCCCACTC TCCTACAAGC ATGACACCAA GCTACTCATC 
CTGGCCCTGG AGAGGCTCAA GGAGGCCTAC AGTGTGAAGT CCCGTTTGAA CCAATCCCAA 
AGAGAGGAGC TGGGCCTGAT TGAGCAAGCC TATGACAACC CGCACGAGGC TCTGTCTCGT 
ATCAAGCGTC ACTTACTCAC GCAGAGAGCC TTCAAGGAGG TGGGCATTGA GTTCATGGAC 
CTGTACAGTC ACCTGGTTCC TGTGTACGAC GTAGAACCCC TCGAGAAGAT CACAGACGCC 
TACTTGGACC AGTACCTGTG GTATGAAGCT GACAAGCGCC GGCTCTTCCC ACCCTGGATC 
AAACCTGCTG ACACGGAGCC GCCTCCCCTG CTGGTGTATA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAG GGAGAGTGCA ACGTCATGCT GGAGTCGCGC 
TTTGAGAAGA TGTACGAGAA GATCGACTTG ACGCTCCTGA ACAGGCTCCT GCGCCTCATT 
GTGGACCACA ACATTGCTGA CTATATGACG GCCAAGAACA ACGTGGTCAT CAACTACAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATTCGAGGAC TTCAGTTCGC CTCCTTCATC 
GTGCAGTACT ACGGGCTGGT GATGGACCTT CTGGTATTGG GCCTACACCG GGCCAGCGAG 
ATGGCTGGGC CTCCCCAAAT GCCCAATGAC TTCCTTAGCT TCCAAGACAT CGCCACAGAA 
GCTGCCCACC CCATCCGCCT TTTCTGTAGA TACATCGACC GCATCCACAT TTTCTTCAGG 
TTCACTGCCG ACGAGGCCCG GGACTTGATC CAGCGCTACC TCACGGAACA CCCTGACCCC 
AACAACGAGA ACATCGTGGG CTACAACAAC AAGAAGTGCT GGCCCCGCGA CGCGCGGATG 
CGCCTCATGA AGCATGACGT CAACTTGGGC CGCGCTGTGT TCTGGGACAT CAAAAACCGA 
CTGCCGCGAT CGGTAACAAC GGTGCAGTGG GAGAACAGCT TTGTGTCAGT GTACAGCAAG 
GACAACCCCA ATCTGCTCTT CAACATGTGC GGCTTCGAGT GCCGCATCCT GCCCAAATGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GATGGTGTTT GGAACCTCCA GAATGAGGTC 
ACAAAGGAGC GGACAGCTCA GTGCTTCCTG CGTGTGGATG ATGAGTCTAT GCAGCGCTTC 
CACAACCGTG TGCGTCAGAT CCTGATGGCC TCTGGCTCGA CCACCTTCAC CAAGATTGTG 
AACAAGTGGA ACACGGCTCT CATTGGGCTC ATGACGTACT TCCGTGAGGC TGTGGTGAAC 
ACCCAGGAGC TGCTGGACCT TCTGGTGAAA TGTGAGAACA AGATCCAGAC CCGAATCAAG 
ATCGGGCTCA ACTCCAAGAT GCCAAGCCGC TTCCCTCCAG TCGTGTTCTA CACCCCCAAA 
GAGCTCGGGG GGCTGGGCAT GTTGTCCATG GGCCACGTGC TCATCCCCCA GTCTGATCTC 
AGGTGGTCCA AGCAGACAGA TGTGGGCATC ACCCACTTCC GCTCCGGGAT GAGCCACGAA 
GAAGACCAGC TCATTCCTAA CTTGTACCGT TACATCCAGC CTTGGGAGAG CGAGTTCATT 
GACTCTCAAC GGGTCTGGGC CGAGTATGCG CTCAAGAGGC AGGAAGCCAT CGCTCAGAAC 
AGGCGGCTGA CCTTGGAGGA CCTGGAGGAT TCATGGGATC GAGGCATTCC CCGGATCAAT 
ACCCTGTTCC AAAAGGACCG GCACACACTG GCTTATGACA AGGGCTGGCG TGTCCGCACT 
GACTTCAAGC AATACCAGGT CCTGAAACAG AATCCTTTCT GGTGGACCCA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAATTAC CGCACAGACA TGATCCAGGC CCTGGGTGGT 
GTGGAGGGCA TCCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGT 
CTCTTCTGGG AGAAGGCCAG TGGTTTTGAG GAGTCGATGA AATGGAAGAA GCTGACCAAT 
GCCCAGCGGT CCGGCTTGAA CCAGATCCCC AACCGTCGCT TCACACTGTG GTGGTCGCCA 
ACCATTAACC GCGCCAATGT GTATGTAGGT TTCCAGGTCC AGCTGGACCT TACTGGCATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTCA TCCAGATTTT CCGTGCCCAT 
TTATGGCAGA AGATCCATGA GAGTATTGTC ATGGACTTGT GTCAGGTGTT TGACCAAGAA 
TTGGATGCGC TGGAAATTGA GACGGTGCAG AAGGAGACAA TTCACCCCCG GAAGTCCTAT 
AAGATGAACT CATCTTGTGC AGACATTCTG CTTTTTGCCT CCTACAAATG GAACGTCTCC 
CGGCCGTCTC TGCTGGCTGA CTCCAAGGAC GTGATGGACA GCACCACCAC TCAGAAGTAC 
TGGATCGACA TCCAGCTGCG CTGGGGCGAC TATGACTCCC ACGATATCGA ACGCTACGCT 
CGGGCCAAGT TTCTGGATTA CACTACCGAC AACATGAGTA TCTACCCCTC TCCAACGGGG 
GTGCTCATTG CCATAGACCT GGCATACAAT CTGCACAGCG CCTATGGAAA CTGGTTTCCA 
GGCAGCAAGC CGCTGATCCA GCAAGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCTTG 
TACGTGCTCC GAGAGCGAAT ACGCAAGGGG TTGCAGCTGT ACTCCTCCGA GCCCACAGAG 
CCTTACCTCT CCTCCCAGAA CTATGGCGAG CTCTTCTCCA ACCAGATCAT CTGGTTTGTA 
GATGACACCA ATGTCTACAG AGTCACCATT CACAAGACTT TTGAAGGGAA CTTGACTACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AATCCAAGGA CGGGGCAGCT CTTCCTGAAG 
ATCATCCACA CGTCTGTGTG GGCTGGACAG AAGCGTCTGG GTCAGTTGGC CAAGTGGAAG 
ACAGCCGAGG AAGTGGCTGC CCTGATCCGT TCTCTCCCTG TAGAAGAGCA GCCCAAACAG 
ATTATTGTGA CCAGAAAAGG CATGTTGGAC CCCTTGGAGG TGCACTTACT GGACTTCCCC 
AACATTGTGA TCAAAGGCTC GGAGCTGCAG CTGCCCTTCC AGGCCTGTCT CAAAGTGGAA 
AAATTCGGGG ACCTTATCCT CAAGGCCACC GAGCCCCAAA TGGTTCTCTT CAATCTCTAT 
GACGACTGGC TCAGAACCAT CTCATCCTAC ACGGCCTTTT CCCGTCTGAT CCTGATTCTC 
CGTGCCCTTC ATGTGAACAA TGACCGGGCC AAAGTGATCT TGAAGCCAGA CAAGACCACC 
ATCACAGAGC CGCACCACAT TTGGCCCACG CTGACCGACG AGGAGTGGAT CAAGGTTGAG 
GTGCAGCTCA AGGACTTGAT CCTGGCTGAT TACGGCAAAA AGAACAACGT CAACGTGGCC 
TCACTGACAC AATCTGAGAT CCGGGACATC ATTCTGGGCA TGGAAATCTC CGCCCCTTCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAGCAGTC CCAGCTGACG 
GCAACACAGA CCCGGACTGT CAACAAGCAC GGTGATGAGA TCATCACTTC CACCACCAGC 
AACTACGAGA CACAAACTTT TTCCTCGAAG ACCGAGTGGA GGGTCAGGGC CATCTCTGCA 
GCCAACCTGC ATCTGCGGAC CAACCACATC TACGTGTCAT CTGATGACAT CAAAGAGACA 
GGCTACACTT ACATCCTTCC CAAGAATGTC CTCAAGAAAT TCATCTGCAT TTCTGATCTT 
CGGGCCCAGA TTGCAGGCTA CCTCTATGGA GTGAGCCCCC CGGACAACCC TCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTGCCTCAG TGGGGCACCC ACCAGACGGT GCACCTGCCG 
GGCCAGCTTC CCCAACACGA GTACCTCAAG GAGATGGAGC CCCTCGGCTG GATCCACACA 
CAGCCCAACG AGTCCCCCCA GCTCTCGCCC CAGGATGTCA CCACCCATGC CAAGGTCATG 
GCCGACAACC CCTCATGGGA CGGGGAGAAG ACAATCATCA TCACCTGCAG CTTTACCCCG 
GGCTCTTGCA CCCTGACGGC CTACAAGCTG ACGCCCAGTG GTTACGAGTG GGGTCGGCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACTTGCCTT CCCACTATGA GCGTGTCCAG 
ATGCTGCTCT CTGACCGCTT CCTTGGCTTC TTCATGGTCC CCGCTCAGGC GTCCTGGAAC 
TACAATTTCA TGGGTGTTCG GCACGACCCC AACATGAAAT ACGAGCTGCA GCTGGCCAAC 
CCCAAAGAGT TCTATCACGA GGTGCACCGG CCCTCCCACT TCCTGAACTT CGCGCTGCTG 
CAGGAGGGGG AGGTGTACTC GGCCGACCGA GAGGACCTGT ACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF8 ( XM_023503711.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023359479.2 Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.

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CloneID	OSa234804
Clone ID Related Accession (Same CDS sequence)	XM_031967785.1 , XM_023503711.2 , XM_031967786.1
Accession Version	XM_023503711.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_023503711.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG TGTTTCCCTA CAGAGGGCCG GGGACCCCCG TGCCCGGCCC TCTGGCACCG 
CTGCCCGACT ACATGTCAGA GGAGAAGCTT CAGGAAAAAG CCCGAAAATG GCAGCAGTTG 
CAGGCAAAAC GCTATGCAGA GAAGCGGAAA TTTGGATTTG TAGACGCGCA AAAGGAAGAC 
ATGCCTCCAG AGCACGTCAG GAAGATCATC CGGGACCATG GGGACATGAC GAACCGCAAG 
TTCCGTCATG ACAAGCGAGT CTACCTGGGG GCCCTGAAGT ACATGCCGCA TGCTGTCCTC 
AAGCTCTTGG AAAACATGCC CATGCCCTGG GAGCAGATCC GGGATGTGCC TGTTCTGTAC 
CACATCACTG GTGCGATTTC CTTTGTTAAT GAGATTCCTT GGGTCATCGA GCCTGTCTAC 
ATCTCTCAGT GGGGGTCCAT GTGGATCATG ATGCGTCGAG AGAAGAGGGA CAGGCGGCAC 
TTCAAGCGTA TGCGTTTTCC CCCATTTGAT GATGAGGAGC CGCCCCTTGA CTATGCTGAC 
AACATCCTCG ATGTGGAGCC TTTGGAAGCC ATTCAGCTGG AACTGGACCC TGAGGAGGAT 
GCCCCTGTGC TAGACTGGTT TTATGACCAC CAGCCCTTAA GAGACAGTAG GAAGTACGTG 
AATGGCTCTA CTTATCAGCG CTGGCAGTTC ACCCTGCCCA TGATGTCCAC TCTGTACCGC 
CTGGCCAATC AGCTCCTAAC TGATCTTGTG GATGACAATT ACTTCTATCT GTTTGACCTA 
AAGGCGTTTT TTACCTCTAA GGCCCTCAAC ATGGCTATCC CAGGGGGCCC TAAATTTGAA 
CCGCTGGTCC GAGATATCAA TCTTCAGGAT GAAGACTGGA ATGAATTTAA TGATATCAAC 
AAGATCATCA TCAGGCAGCC CATCAGAACC GAATATAAGA TTGCTTTTCC ATACTTGTAC 
AACAACCTTC CACACCACGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACAGAGG ACCCTGATCT TCCAGCCTTC TACTTTGATC CTTTGATCAA TCCTATCTCC 
CACAGACATT CTGTCAAGAG TCAAGAACCT TTGCCAGATG ATGACGAGGA GTTTGAGCTG 
CCCGAGTTTG TGGAACCTTT CCTCAAGGAC ACGCCCCTTT ACACAGATAA TACTGCCAAT 
GGCATCGCCC TTCTGTGGGC ACCTAGGCCC TTCAACCTTC GCTCTGGTCG CACCCGCCGT 
GCCCTGGACA TACCCCTGGT TAAAAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAGGTGA GGGTCTCCTA CCAAAAGCTA CTCAAATACT ACGTGCTGAA TGCCCTCAAG 
CACCGACCCC CCAAGGCCCA GAAAAAGAGG TATCTCTTCC GATCCTTTAA GGCCACCAAG 
TTCTTCCAGT CTACCAAGCT GGACTGGGTG GAGGTAGGGC TGCAGGTCTG CCGCCAGGGC 
TACAATATGC TCAACCTCCT CATCCACCGG AAGAATCTCA ACTACTTGCA TCTGGACTAT 
AACTTCAACC TAAAGCCTGT CAAGACTCTC ACCACCAAGG AAAGAAAGAA GTCTCGCTTT 
GGAAATGCCT TCCACTTGTG TCGGGAGGTG CTGCGGCTGA CCAAGCTCGT AGTGGACAGC 
CACGTTCAGT ACCGCTTGGG CAACGTGGAT GCCTTCCAGC TAGCGGACGG GCTGCAGTAT 
ATATTTGCCC ACGTGGGACA GCTGACAGGA ATGTACCGCT ACAAGTACAA GTTGATGCGG 
CAGATCCGCA TGTGCAAGGA CCTGAAGCAC CTTATCTACT ACCGCTTCAA CACGGGTCCC 
GTGGGGAAGG GCCCAGGCTG TGGTTTCTGG GCTGCTGGTT GGCGAGTCTG GTTGTTTTTC 
ATGCGTGGCA TCACCCCTCT TTTGGAACGG TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGTC ACTCAAAGGG GGTGGCAAAG ACCGTGACCA AGCAGCGAGT GGAGTCCCAC 
TTTGACTTGG AGCTCCGGGC AGCGGTGATG CATGATATCT TGGATATGAT GCCTGAGGGC 
ATCAAACAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TCCCCTGGAA GGTTCCTGGG TTGCCGACCC CCATTGAGAA CATGATCCTG 
CGCTACGTGA AGGCGAAGGC GGATTGGTGG ACCAACACCG CCCATTACAA CCGAGAGCGG 
ATCCGCCGGG GAGCCACCGT CGACAAGACC GTTTGTAAGA AGAACTTGGG TCGGCTCACA 
CGGCTCTATC TGAAGGCTGA ACAGGAACGA CAGCACAACT ACCTAAAGGA TGGTCCCTAC 
ATCACAGCAG AAGAGGCAGT GGCTGTCTAC ACCACCACTG TGCACTGGCT GGAGAGCCGC 
AGATTCTCCC CCATCCCTTT CCCCCCACTC TCCTACAAGC ATGACACCAA GCTACTCATC 
CTGGCCCTGG AGAGGCTCAA GGAGGCCTAC AGTGTGAAGT CCCGTTTGAA CCAATCCCAA 
AGAGAGGAGC TGGGCCTGAT TGAGCAAGCC TATGACAACC CGCACGAGGC TCTGTCTCGT 
ATCAAGCGTC ACTTACTCAC GCAGAGAGCC TTCAAGGAGG TGGGCATTGA GTTCATGGAC 
CTGTACAGTC ACCTGGTTCC TGTGTACGAC GTAGAACCCC TCGAGAAGAT CACAGACGCC 
TACTTGGACC AGTACCTGTG GTATGAAGCT GACAAGCGCC GGCTCTTCCC ACCCTGGATC 
AAACCTGCTG ACACGGAGCC GCCTCCCCTG CTGGTGTATA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAG GGAGAGTGCA ACGTCATGCT GGAGTCGCGC 
TTTGAGAAGA TGTACGAGAA GATCGACTTG ACGCTCCTGA ACAGGCTCCT GCGCCTCATT 
GTGGACCACA ACATTGCTGA CTATATGACG GCCAAGAACA ACGTGGTCAT CAACTACAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATTCGAGGAC TTCAGTTCGC CTCCTTCATC 
GTGCAGTACT ACGGGCTGGT GATGGACCTT CTGGTATTGG GCCTACACCG GGCCAGCGAG 
ATGGCTGGGC CTCCCCAAAT GCCCAATGAC TTCCTTAGCT TCCAAGACAT CGCCACAGAA 
GCTGCCCACC CCATCCGCCT TTTCTGTAGA TACATCGACC GCATCCACAT TTTCTTCAGG 
TTCACTGCCG ACGAGGCCCG GGACTTGATC CAGCGCTACC TCACGGAACA CCCTGACCCC 
AACAACGAGA ACATCGTGGG CTACAACAAC AAGAAGTGCT GGCCCCGCGA CGCGCGGATG 
CGCCTCATGA AGCATGACGT CAACTTGGGC CGCGCTGTGT TCTGGGACAT CAAAAACCGA 
CTGCCGCGAT CGGTAACAAC GGTGCAGTGG GAGAACAGCT TTGTGTCAGT GTACAGCAAG 
GACAACCCCA ATCTGCTCTT CAACATGTGC GGCTTCGAGT GCCGCATCCT GCCCAAATGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GATGGTGTTT GGAACCTCCA GAATGAGGTC 
ACAAAGGAGC GGACAGCTCA GTGCTTCCTG CGTGTGGATG ATGAGTCTAT GCAGCGCTTC 
CACAACCGTG TGCGTCAGAT CCTGATGGCC TCTGGCTCGA CCACCTTCAC CAAGATTGTG 
AACAAGTGGA ACACGGCTCT CATTGGGCTC ATGACGTACT TCCGTGAGGC TGTGGTGAAC 
ACCCAGGAGC TGCTGGACCT TCTGGTGAAA TGTGAGAACA AGATCCAGAC CCGAATCAAG 
ATCGGGCTCA ACTCCAAGAT GCCAAGCCGC TTCCCTCCAG TCGTGTTCTA CACCCCCAAA 
GAGCTCGGGG GGCTGGGCAT GTTGTCCATG GGCCACGTGC TCATCCCCCA GTCTGATCTC 
AGGTGGTCCA AGCAGACAGA TGTGGGCATC ACCCACTTCC GCTCCGGGAT GAGCCACGAA 
GAAGACCAGC TCATTCCTAA CTTGTACCGT TACATCCAGC CTTGGGAGAG CGAGTTCATT 
GACTCTCAAC GGGTCTGGGC CGAGTATGCG CTCAAGAGGC AGGAAGCCAT CGCTCAGAAC 
AGGCGGCTGA CCTTGGAGGA CCTGGAGGAT TCATGGGATC GAGGCATTCC CCGGATCAAT 
ACCCTGTTCC AAAAGGACCG GCACACACTG GCTTATGACA AGGGCTGGCG TGTCCGCACT 
GACTTCAAGC AATACCAGGT CCTGAAACAG AATCCTTTCT GGTGGACCCA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAATTAC CGCACAGACA TGATCCAGGC CCTGGGTGGT 
GTGGAGGGCA TCCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGT 
CTCTTCTGGG AGAAGGCCAG TGGTTTTGAG GAGTCGATGA AATGGAAGAA GCTGACCAAT 
GCCCAGCGGT CCGGCTTGAA CCAGATCCCC AACCGTCGCT TCACACTGTG GTGGTCGCCA 
ACCATTAACC GCGCCAATGT GTATGTAGGT TTCCAGGTCC AGCTGGACCT TACTGGCATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTCA TCCAGATTTT CCGTGCCCAT 
TTATGGCAGA AGATCCATGA GAGTATTGTC ATGGACTTGT GTCAGGTGTT TGACCAAGAA 
TTGGATGCGC TGGAAATTGA GACGGTGCAG AAGGAGACAA TTCACCCCCG GAAGTCCTAT 
AAGATGAACT CATCTTGTGC AGACATTCTG CTTTTTGCCT CCTACAAATG GAACGTCTCC 
CGGCCGTCTC TGCTGGCTGA CTCCAAGGAC GTGATGGACA GCACCACCAC TCAGAAGTAC 
TGGATCGACA TCCAGCTGCG CTGGGGCGAC TATGACTCCC ACGATATCGA ACGCTACGCT 
CGGGCCAAGT TTCTGGATTA CACTACCGAC AACATGAGTA TCTACCCCTC TCCAACGGGG 
GTGCTCATTG CCATAGACCT GGCATACAAT CTGCACAGCG CCTATGGAAA CTGGTTTCCA 
GGCAGCAAGC CGCTGATCCA GCAAGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCTTG 
TACGTGCTCC GAGAGCGAAT ACGCAAGGGG TTGCAGCTGT ACTCCTCCGA GCCCACAGAG 
CCTTACCTCT CCTCCCAGAA CTATGGCGAG CTCTTCTCCA ACCAGATCAT CTGGTTTGTA 
GATGACACCA ATGTCTACAG AGTCACCATT CACAAGACTT TTGAAGGGAA CTTGACTACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AATCCAAGGA CGGGGCAGCT CTTCCTGAAG 
ATCATCCACA CGTCTGTGTG GGCTGGACAG AAGCGTCTGG GTCAGTTGGC CAAGTGGAAG 
ACAGCCGAGG AAGTGGCTGC CCTGATCCGT TCTCTCCCTG TAGAAGAGCA GCCCAAACAG 
ATTATTGTGA CCAGAAAAGG CATGTTGGAC CCCTTGGAGG TGCACTTACT GGACTTCCCC 
AACATTGTGA TCAAAGGCTC GGAGCTGCAG CTGCCCTTCC AGGCCTGTCT CAAAGTGGAA 
AAATTCGGGG ACCTTATCCT CAAGGCCACC GAGCCCCAAA TGGTTCTCTT CAATCTCTAT 
GACGACTGGC TCAGAACCAT CTCATCCTAC ACGGCCTTTT CCCGTCTGAT CCTGATTCTC 
CGTGCCCTTC ATGTGAACAA TGACCGGGCC AAAGTGATCT TGAAGCCAGA CAAGACCACC 
ATCACAGAGC CGCACCACAT TTGGCCCACG CTGACCGACG AGGAGTGGAT CAAGGTTGAG 
GTGCAGCTCA AGGACTTGAT CCTGGCTGAT TACGGCAAAA AGAACAACGT CAACGTGGCC 
TCACTGACAC AATCTGAGAT CCGGGACATC ATTCTGGGCA TGGAAATCTC CGCCCCTTCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAGCAGTC CCAGCTGACG 
GCAACACAGA CCCGGACTGT CAACAAGCAC GGTGATGAGA TCATCACTTC CACCACCAGC 
AACTACGAGA CACAAACTTT TTCCTCGAAG ACCGAGTGGA GGGTCAGGGC CATCTCTGCA 
GCCAACCTGC ATCTGCGGAC CAACCACATC TACGTGTCAT CTGATGACAT CAAAGAGACA 
GGCTACACTT ACATCCTTCC CAAGAATGTC CTCAAGAAAT TCATCTGCAT TTCTGATCTT 
CGGGCCCAGA TTGCAGGCTA CCTCTATGGA GTGAGCCCCC CGGACAACCC TCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTGCCTCAG TGGGGCACCC ACCAGACGGT GCACCTGCCG 
GGCCAGCTTC CCCAACACGA GTACCTCAAG GAGATGGAGC CCCTCGGCTG GATCCACACA 
CAGCCCAACG AGTCCCCCCA GCTCTCGCCC CAGGATGTCA CCACCCATGC CAAGGTCATG 
GCCGACAACC CCTCATGGGA CGGGGAGAAG ACAATCATCA TCACCTGCAG CTTTACCCCG 
GGCTCTTGCA CCCTGACGGC CTACAAGCTG ACGCCCAGTG GTTACGAGTG GGGTCGGCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACTTGCCTT CCCACTATGA GCGTGTCCAG 
ATGCTGCTCT CTGACCGCTT CCTTGGCTTC TTCATGGTCC CCGCTCAGGC GTCCTGGAAC 
TACAATTTCA TGGGTGTTCG GCACGACCCC AACATGAAAT ACGAGCTGCA GCTGGCCAAC 
CCCAAAGAGT TCTATCACGA GGTGCACCGG CCCTCCCACT TCCTGAACTT CGCGCTGCTG 
CAGGAGGGGG AGGTGTACTC GGCCGACCGA GAGGACCTGT ACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023359479.2
CDS	106..7113
Translation	MAGVFPYRGPGTPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLRTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKVMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQASWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_023503711.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023503711.2

ORF Insert Sequence:

ATGGCCGGGG TGTTTCCCTA CAGAGGGCCG GGGACCCCCG TGCCCGGCCC TCTGGCACCG 
CTGCCCGACT ACATGTCAGA GGAGAAGCTT CAGGAAAAAG CCCGAAAATG GCAGCAGTTG 
CAGGCAAAAC GCTATGCAGA GAAGCGGAAA TTTGGATTTG TAGACGCGCA AAAGGAAGAC 
ATGCCTCCAG AGCACGTCAG GAAGATCATC CGGGACCATG GGGACATGAC GAACCGCAAG 
TTCCGTCATG ACAAGCGAGT CTACCTGGGG GCCCTGAAGT ACATGCCGCA TGCTGTCCTC 
AAGCTCTTGG AAAACATGCC CATGCCCTGG GAGCAGATCC GGGATGTGCC TGTTCTGTAC 
CACATCACTG GTGCGATTTC CTTTGTTAAT GAGATTCCTT GGGTCATCGA GCCTGTCTAC 
ATCTCTCAGT GGGGGTCCAT GTGGATCATG ATGCGTCGAG AGAAGAGGGA CAGGCGGCAC 
TTCAAGCGTA TGCGTTTTCC CCCATTTGAT GATGAGGAGC CGCCCCTTGA CTATGCTGAC 
AACATCCTCG ATGTGGAGCC TTTGGAAGCC ATTCAGCTGG AACTGGACCC TGAGGAGGAT 
GCCCCTGTGC TAGACTGGTT TTATGACCAC CAGCCCTTAA GAGACAGTAG GAAGTACGTG 
AATGGCTCTA CTTATCAGCG CTGGCAGTTC ACCCTGCCCA TGATGTCCAC TCTGTACCGC 
CTGGCCAATC AGCTCCTAAC TGATCTTGTG GATGACAATT ACTTCTATCT GTTTGACCTA 
AAGGCGTTTT TTACCTCTAA GGCCCTCAAC ATGGCTATCC CAGGGGGCCC TAAATTTGAA 
CCGCTGGTCC GAGATATCAA TCTTCAGGAT GAAGACTGGA ATGAATTTAA TGATATCAAC 
AAGATCATCA TCAGGCAGCC CATCAGAACC GAATATAAGA TTGCTTTTCC ATACTTGTAC 
AACAACCTTC CACACCACGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACAGAGG ACCCTGATCT TCCAGCCTTC TACTTTGATC CTTTGATCAA TCCTATCTCC 
CACAGACATT CTGTCAAGAG TCAAGAACCT TTGCCAGATG ATGACGAGGA GTTTGAGCTG 
CCCGAGTTTG TGGAACCTTT CCTCAAGGAC ACGCCCCTTT ACACAGATAA TACTGCCAAT 
GGCATCGCCC TTCTGTGGGC ACCTAGGCCC TTCAACCTTC GCTCTGGTCG CACCCGCCGT 
GCCCTGGACA TACCCCTGGT TAAAAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAGGTGA GGGTCTCCTA CCAAAAGCTA CTCAAATACT ACGTGCTGAA TGCCCTCAAG 
CACCGACCCC CCAAGGCCCA GAAAAAGAGG TATCTCTTCC GATCCTTTAA GGCCACCAAG 
TTCTTCCAGT CTACCAAGCT GGACTGGGTG GAGGTAGGGC TGCAGGTCTG CCGCCAGGGC 
TACAATATGC TCAACCTCCT CATCCACCGG AAGAATCTCA ACTACTTGCA TCTGGACTAT 
AACTTCAACC TAAAGCCTGT CAAGACTCTC ACCACCAAGG AAAGAAAGAA GTCTCGCTTT 
GGAAATGCCT TCCACTTGTG TCGGGAGGTG CTGCGGCTGA CCAAGCTCGT AGTGGACAGC 
CACGTTCAGT ACCGCTTGGG CAACGTGGAT GCCTTCCAGC TAGCGGACGG GCTGCAGTAT 
ATATTTGCCC ACGTGGGACA GCTGACAGGA ATGTACCGCT ACAAGTACAA GTTGATGCGG 
CAGATCCGCA TGTGCAAGGA CCTGAAGCAC CTTATCTACT ACCGCTTCAA CACGGGTCCC 
GTGGGGAAGG GCCCAGGCTG TGGTTTCTGG GCTGCTGGTT GGCGAGTCTG GTTGTTTTTC 
ATGCGTGGCA TCACCCCTCT TTTGGAACGG TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGTC ACTCAAAGGG GGTGGCAAAG ACCGTGACCA AGCAGCGAGT GGAGTCCCAC 
TTTGACTTGG AGCTCCGGGC AGCGGTGATG CATGATATCT TGGATATGAT GCCTGAGGGC 
ATCAAACAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TCCCCTGGAA GGTTCCTGGG TTGCCGACCC CCATTGAGAA CATGATCCTG 
CGCTACGTGA AGGCGAAGGC GGATTGGTGG ACCAACACCG CCCATTACAA CCGAGAGCGG 
ATCCGCCGGG GAGCCACCGT CGACAAGACC GTTTGTAAGA AGAACTTGGG TCGGCTCACA 
CGGCTCTATC TGAAGGCTGA ACAGGAACGA CAGCACAACT ACCTAAAGGA TGGTCCCTAC 
ATCACAGCAG AAGAGGCAGT GGCTGTCTAC ACCACCACTG TGCACTGGCT GGAGAGCCGC 
AGATTCTCCC CCATCCCTTT CCCCCCACTC TCCTACAAGC ATGACACCAA GCTACTCATC 
CTGGCCCTGG AGAGGCTCAA GGAGGCCTAC AGTGTGAAGT CCCGTTTGAA CCAATCCCAA 
AGAGAGGAGC TGGGCCTGAT TGAGCAAGCC TATGACAACC CGCACGAGGC TCTGTCTCGT 
ATCAAGCGTC ACTTACTCAC GCAGAGAGCC TTCAAGGAGG TGGGCATTGA GTTCATGGAC 
CTGTACAGTC ACCTGGTTCC TGTGTACGAC GTAGAACCCC TCGAGAAGAT CACAGACGCC 
TACTTGGACC AGTACCTGTG GTATGAAGCT GACAAGCGCC GGCTCTTCCC ACCCTGGATC 
AAACCTGCTG ACACGGAGCC GCCTCCCCTG CTGGTGTATA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAG GGAGAGTGCA ACGTCATGCT GGAGTCGCGC 
TTTGAGAAGA TGTACGAGAA GATCGACTTG ACGCTCCTGA ACAGGCTCCT GCGCCTCATT 
GTGGACCACA ACATTGCTGA CTATATGACG GCCAAGAACA ACGTGGTCAT CAACTACAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATTCGAGGAC TTCAGTTCGC CTCCTTCATC 
GTGCAGTACT ACGGGCTGGT GATGGACCTT CTGGTATTGG GCCTACACCG GGCCAGCGAG 
ATGGCTGGGC CTCCCCAAAT GCCCAATGAC TTCCTTAGCT TCCAAGACAT CGCCACAGAA 
GCTGCCCACC CCATCCGCCT TTTCTGTAGA TACATCGACC GCATCCACAT TTTCTTCAGG 
TTCACTGCCG ACGAGGCCCG GGACTTGATC CAGCGCTACC TCACGGAACA CCCTGACCCC 
AACAACGAGA ACATCGTGGG CTACAACAAC AAGAAGTGCT GGCCCCGCGA CGCGCGGATG 
CGCCTCATGA AGCATGACGT CAACTTGGGC CGCGCTGTGT TCTGGGACAT CAAAAACCGA 
CTGCCGCGAT CGGTAACAAC GGTGCAGTGG GAGAACAGCT TTGTGTCAGT GTACAGCAAG 
GACAACCCCA ATCTGCTCTT CAACATGTGC GGCTTCGAGT GCCGCATCCT GCCCAAATGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GATGGTGTTT GGAACCTCCA GAATGAGGTC 
ACAAAGGAGC GGACAGCTCA GTGCTTCCTG CGTGTGGATG ATGAGTCTAT GCAGCGCTTC 
CACAACCGTG TGCGTCAGAT CCTGATGGCC TCTGGCTCGA CCACCTTCAC CAAGATTGTG 
AACAAGTGGA ACACGGCTCT CATTGGGCTC ATGACGTACT TCCGTGAGGC TGTGGTGAAC 
ACCCAGGAGC TGCTGGACCT TCTGGTGAAA TGTGAGAACA AGATCCAGAC CCGAATCAAG 
ATCGGGCTCA ACTCCAAGAT GCCAAGCCGC TTCCCTCCAG TCGTGTTCTA CACCCCCAAA 
GAGCTCGGGG GGCTGGGCAT GTTGTCCATG GGCCACGTGC TCATCCCCCA GTCTGATCTC 
AGGTGGTCCA AGCAGACAGA TGTGGGCATC ACCCACTTCC GCTCCGGGAT GAGCCACGAA 
GAAGACCAGC TCATTCCTAA CTTGTACCGT TACATCCAGC CTTGGGAGAG CGAGTTCATT 
GACTCTCAAC GGGTCTGGGC CGAGTATGCG CTCAAGAGGC AGGAAGCCAT CGCTCAGAAC 
AGGCGGCTGA CCTTGGAGGA CCTGGAGGAT TCATGGGATC GAGGCATTCC CCGGATCAAT 
ACCCTGTTCC AAAAGGACCG GCACACACTG GCTTATGACA AGGGCTGGCG TGTCCGCACT 
GACTTCAAGC AATACCAGGT CCTGAAACAG AATCCTTTCT GGTGGACCCA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAATTAC CGCACAGACA TGATCCAGGC CCTGGGTGGT 
GTGGAGGGCA TCCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGT 
CTCTTCTGGG AGAAGGCCAG TGGTTTTGAG GAGTCGATGA AATGGAAGAA GCTGACCAAT 
GCCCAGCGGT CCGGCTTGAA CCAGATCCCC AACCGTCGCT TCACACTGTG GTGGTCGCCA 
ACCATTAACC GCGCCAATGT GTATGTAGGT TTCCAGGTCC AGCTGGACCT TACTGGCATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTCA TCCAGATTTT CCGTGCCCAT 
TTATGGCAGA AGATCCATGA GAGTATTGTC ATGGACTTGT GTCAGGTGTT TGACCAAGAA 
TTGGATGCGC TGGAAATTGA GACGGTGCAG AAGGAGACAA TTCACCCCCG GAAGTCCTAT 
AAGATGAACT CATCTTGTGC AGACATTCTG CTTTTTGCCT CCTACAAATG GAACGTCTCC 
CGGCCGTCTC TGCTGGCTGA CTCCAAGGAC GTGATGGACA GCACCACCAC TCAGAAGTAC 
TGGATCGACA TCCAGCTGCG CTGGGGCGAC TATGACTCCC ACGATATCGA ACGCTACGCT 
CGGGCCAAGT TTCTGGATTA CACTACCGAC AACATGAGTA TCTACCCCTC TCCAACGGGG 
GTGCTCATTG CCATAGACCT GGCATACAAT CTGCACAGCG CCTATGGAAA CTGGTTTCCA 
GGCAGCAAGC CGCTGATCCA GCAAGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCTTG 
TACGTGCTCC GAGAGCGAAT ACGCAAGGGG TTGCAGCTGT ACTCCTCCGA GCCCACAGAG 
CCTTACCTCT CCTCCCAGAA CTATGGCGAG CTCTTCTCCA ACCAGATCAT CTGGTTTGTA 
GATGACACCA ATGTCTACAG AGTCACCATT CACAAGACTT TTGAAGGGAA CTTGACTACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AATCCAAGGA CGGGGCAGCT CTTCCTGAAG 
ATCATCCACA CGTCTGTGTG GGCTGGACAG AAGCGTCTGG GTCAGTTGGC CAAGTGGAAG 
ACAGCCGAGG AAGTGGCTGC CCTGATCCGT TCTCTCCCTG TAGAAGAGCA GCCCAAACAG 
ATTATTGTGA CCAGAAAAGG CATGTTGGAC CCCTTGGAGG TGCACTTACT GGACTTCCCC 
AACATTGTGA TCAAAGGCTC GGAGCTGCAG CTGCCCTTCC AGGCCTGTCT CAAAGTGGAA 
AAATTCGGGG ACCTTATCCT CAAGGCCACC GAGCCCCAAA TGGTTCTCTT CAATCTCTAT 
GACGACTGGC TCAGAACCAT CTCATCCTAC ACGGCCTTTT CCCGTCTGAT CCTGATTCTC 
CGTGCCCTTC ATGTGAACAA TGACCGGGCC AAAGTGATCT TGAAGCCAGA CAAGACCACC 
ATCACAGAGC CGCACCACAT TTGGCCCACG CTGACCGACG AGGAGTGGAT CAAGGTTGAG 
GTGCAGCTCA AGGACTTGAT CCTGGCTGAT TACGGCAAAA AGAACAACGT CAACGTGGCC 
TCACTGACAC AATCTGAGAT CCGGGACATC ATTCTGGGCA TGGAAATCTC CGCCCCTTCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAGCAGTC CCAGCTGACG 
GCAACACAGA CCCGGACTGT CAACAAGCAC GGTGATGAGA TCATCACTTC CACCACCAGC 
AACTACGAGA CACAAACTTT TTCCTCGAAG ACCGAGTGGA GGGTCAGGGC CATCTCTGCA 
GCCAACCTGC ATCTGCGGAC CAACCACATC TACGTGTCAT CTGATGACAT CAAAGAGACA 
GGCTACACTT ACATCCTTCC CAAGAATGTC CTCAAGAAAT TCATCTGCAT TTCTGATCTT 
CGGGCCCAGA TTGCAGGCTA CCTCTATGGA GTGAGCCCCC CGGACAACCC TCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTGCCTCAG TGGGGCACCC ACCAGACGGT GCACCTGCCG 
GGCCAGCTTC CCCAACACGA GTACCTCAAG GAGATGGAGC CCCTCGGCTG GATCCACACA 
CAGCCCAACG AGTCCCCCCA GCTCTCGCCC CAGGATGTCA CCACCCATGC CAAGGTCATG 
GCCGACAACC CCTCATGGGA CGGGGAGAAG ACAATCATCA TCACCTGCAG CTTTACCCCG 
GGCTCTTGCA CCCTGACGGC CTACAAGCTG ACGCCCAGTG GTTACGAGTG GGGTCGGCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACTTGCCTT CCCACTATGA GCGTGTCCAG 
ATGCTGCTCT CTGACCGCTT CCTTGGCTTC TTCATGGTCC CCGCTCAGGC GTCCTGGAAC 
TACAATTTCA TGGGTGTTCG GCACGACCCC AACATGAAAT ACGAGCTGCA GCTGGCCAAC 
CCCAAAGAGT TCTATCACGA GGTGCACCGG CCCTCCCACT TCCTGAACTT CGCGCTGCTG 
CAGGAGGGGG AGGTGTACTC GGCCGACCGA GAGGACCTGT ACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF8 ( XM_031967786.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823646.1 Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), transcript variant X3, mRNA.

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CloneID	OSa234804
Clone ID Related Accession (Same CDS sequence)	XM_031967785.1 , XM_023503711.2 , XM_031967786.1
Accession Version	XM_031967786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG TGTTTCCCTA CAGAGGGCCG GGGACCCCCG TGCCCGGCCC TCTGGCACCG 
CTGCCCGACT ACATGTCAGA GGAGAAGCTT CAGGAAAAAG CCCGAAAATG GCAGCAGTTG 
CAGGCAAAAC GCTATGCAGA GAAGCGGAAA TTTGGATTTG TAGACGCGCA AAAGGAAGAC 
ATGCCTCCAG AGCACGTCAG GAAGATCATC CGGGACCATG GGGACATGAC GAACCGCAAG 
TTCCGTCATG ACAAGCGAGT CTACCTGGGG GCCCTGAAGT ACATGCCGCA TGCTGTCCTC 
AAGCTCTTGG AAAACATGCC CATGCCCTGG GAGCAGATCC GGGATGTGCC TGTTCTGTAC 
CACATCACTG GTGCGATTTC CTTTGTTAAT GAGATTCCTT GGGTCATCGA GCCTGTCTAC 
ATCTCTCAGT GGGGGTCCAT GTGGATCATG ATGCGTCGAG AGAAGAGGGA CAGGCGGCAC 
TTCAAGCGTA TGCGTTTTCC CCCATTTGAT GATGAGGAGC CGCCCCTTGA CTATGCTGAC 
AACATCCTCG ATGTGGAGCC TTTGGAAGCC ATTCAGCTGG AACTGGACCC TGAGGAGGAT 
GCCCCTGTGC TAGACTGGTT TTATGACCAC CAGCCCTTAA GAGACAGTAG GAAGTACGTG 
AATGGCTCTA CTTATCAGCG CTGGCAGTTC ACCCTGCCCA TGATGTCCAC TCTGTACCGC 
CTGGCCAATC AGCTCCTAAC TGATCTTGTG GATGACAATT ACTTCTATCT GTTTGACCTA 
AAGGCGTTTT TTACCTCTAA GGCCCTCAAC ATGGCTATCC CAGGGGGCCC TAAATTTGAA 
CCGCTGGTCC GAGATATCAA TCTTCAGGAT GAAGACTGGA ATGAATTTAA TGATATCAAC 
AAGATCATCA TCAGGCAGCC CATCAGAACC GAATATAAGA TTGCTTTTCC ATACTTGTAC 
AACAACCTTC CACACCACGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACAGAGG ACCCTGATCT TCCAGCCTTC TACTTTGATC CTTTGATCAA TCCTATCTCC 
CACAGACATT CTGTCAAGAG TCAAGAACCT TTGCCAGATG ATGACGAGGA GTTTGAGCTG 
CCCGAGTTTG TGGAACCTTT CCTCAAGGAC ACGCCCCTTT ACACAGATAA TACTGCCAAT 
GGCATCGCCC TTCTGTGGGC ACCTAGGCCC TTCAACCTTC GCTCTGGTCG CACCCGCCGT 
GCCCTGGACA TACCCCTGGT TAAAAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAGGTGA GGGTCTCCTA CCAAAAGCTA CTCAAATACT ACGTGCTGAA TGCCCTCAAG 
CACCGACCCC CCAAGGCCCA GAAAAAGAGG TATCTCTTCC GATCCTTTAA GGCCACCAAG 
TTCTTCCAGT CTACCAAGCT GGACTGGGTG GAGGTAGGGC TGCAGGTCTG CCGCCAGGGC 
TACAATATGC TCAACCTCCT CATCCACCGG AAGAATCTCA ACTACTTGCA TCTGGACTAT 
AACTTCAACC TAAAGCCTGT CAAGACTCTC ACCACCAAGG AAAGAAAGAA GTCTCGCTTT 
GGAAATGCCT TCCACTTGTG TCGGGAGGTG CTGCGGCTGA CCAAGCTCGT AGTGGACAGC 
CACGTTCAGT ACCGCTTGGG CAACGTGGAT GCCTTCCAGC TAGCGGACGG GCTGCAGTAT 
ATATTTGCCC ACGTGGGACA GCTGACAGGA ATGTACCGCT ACAAGTACAA GTTGATGCGG 
CAGATCCGCA TGTGCAAGGA CCTGAAGCAC CTTATCTACT ACCGCTTCAA CACGGGTCCC 
GTGGGGAAGG GCCCAGGCTG TGGTTTCTGG GCTGCTGGTT GGCGAGTCTG GTTGTTTTTC 
ATGCGTGGCA TCACCCCTCT TTTGGAACGG TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGTC ACTCAAAGGG GGTGGCAAAG ACCGTGACCA AGCAGCGAGT GGAGTCCCAC 
TTTGACTTGG AGCTCCGGGC AGCGGTGATG CATGATATCT TGGATATGAT GCCTGAGGGC 
ATCAAACAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TCCCCTGGAA GGTTCCTGGG TTGCCGACCC CCATTGAGAA CATGATCCTG 
CGCTACGTGA AGGCGAAGGC GGATTGGTGG ACCAACACCG CCCATTACAA CCGAGAGCGG 
ATCCGCCGGG GAGCCACCGT CGACAAGACC GTTTGTAAGA AGAACTTGGG TCGGCTCACA 
CGGCTCTATC TGAAGGCTGA ACAGGAACGA CAGCACAACT ACCTAAAGGA TGGTCCCTAC 
ATCACAGCAG AAGAGGCAGT GGCTGTCTAC ACCACCACTG TGCACTGGCT GGAGAGCCGC 
AGATTCTCCC CCATCCCTTT CCCCCCACTC TCCTACAAGC ATGACACCAA GCTACTCATC 
CTGGCCCTGG AGAGGCTCAA GGAGGCCTAC AGTGTGAAGT CCCGTTTGAA CCAATCCCAA 
AGAGAGGAGC TGGGCCTGAT TGAGCAAGCC TATGACAACC CGCACGAGGC TCTGTCTCGT 
ATCAAGCGTC ACTTACTCAC GCAGAGAGCC TTCAAGGAGG TGGGCATTGA GTTCATGGAC 
CTGTACAGTC ACCTGGTTCC TGTGTACGAC GTAGAACCCC TCGAGAAGAT CACAGACGCC 
TACTTGGACC AGTACCTGTG GTATGAAGCT GACAAGCGCC GGCTCTTCCC ACCCTGGATC 
AAACCTGCTG ACACGGAGCC GCCTCCCCTG CTGGTGTATA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAG GGAGAGTGCA ACGTCATGCT GGAGTCGCGC 
TTTGAGAAGA TGTACGAGAA GATCGACTTG ACGCTCCTGA ACAGGCTCCT GCGCCTCATT 
GTGGACCACA ACATTGCTGA CTATATGACG GCCAAGAACA ACGTGGTCAT CAACTACAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATTCGAGGAC TTCAGTTCGC CTCCTTCATC 
GTGCAGTACT ACGGGCTGGT GATGGACCTT CTGGTATTGG GCCTACACCG GGCCAGCGAG 
ATGGCTGGGC CTCCCCAAAT GCCCAATGAC TTCCTTAGCT TCCAAGACAT CGCCACAGAA 
GCTGCCCACC CCATCCGCCT TTTCTGTAGA TACATCGACC GCATCCACAT TTTCTTCAGG 
TTCACTGCCG ACGAGGCCCG GGACTTGATC CAGCGCTACC TCACGGAACA CCCTGACCCC 
AACAACGAGA ACATCGTGGG CTACAACAAC AAGAAGTGCT GGCCCCGCGA CGCGCGGATG 
CGCCTCATGA AGCATGACGT CAACTTGGGC CGCGCTGTGT TCTGGGACAT CAAAAACCGA 
CTGCCGCGAT CGGTAACAAC GGTGCAGTGG GAGAACAGCT TTGTGTCAGT GTACAGCAAG 
GACAACCCCA ATCTGCTCTT CAACATGTGC GGCTTCGAGT GCCGCATCCT GCCCAAATGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GATGGTGTTT GGAACCTCCA GAATGAGGTC 
ACAAAGGAGC GGACAGCTCA GTGCTTCCTG CGTGTGGATG ATGAGTCTAT GCAGCGCTTC 
CACAACCGTG TGCGTCAGAT CCTGATGGCC TCTGGCTCGA CCACCTTCAC CAAGATTGTG 
AACAAGTGGA ACACGGCTCT CATTGGGCTC ATGACGTACT TCCGTGAGGC TGTGGTGAAC 
ACCCAGGAGC TGCTGGACCT TCTGGTGAAA TGTGAGAACA AGATCCAGAC CCGAATCAAG 
ATCGGGCTCA ACTCCAAGAT GCCAAGCCGC TTCCCTCCAG TCGTGTTCTA CACCCCCAAA 
GAGCTCGGGG GGCTGGGCAT GTTGTCCATG GGCCACGTGC TCATCCCCCA GTCTGATCTC 
AGGTGGTCCA AGCAGACAGA TGTGGGCATC ACCCACTTCC GCTCCGGGAT GAGCCACGAA 
GAAGACCAGC TCATTCCTAA CTTGTACCGT TACATCCAGC CTTGGGAGAG CGAGTTCATT 
GACTCTCAAC GGGTCTGGGC CGAGTATGCG CTCAAGAGGC AGGAAGCCAT CGCTCAGAAC 
AGGCGGCTGA CCTTGGAGGA CCTGGAGGAT TCATGGGATC GAGGCATTCC CCGGATCAAT 
ACCCTGTTCC AAAAGGACCG GCACACACTG GCTTATGACA AGGGCTGGCG TGTCCGCACT 
GACTTCAAGC AATACCAGGT CCTGAAACAG AATCCTTTCT GGTGGACCCA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAATTAC CGCACAGACA TGATCCAGGC CCTGGGTGGT 
GTGGAGGGCA TCCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGT 
CTCTTCTGGG AGAAGGCCAG TGGTTTTGAG GAGTCGATGA AATGGAAGAA GCTGACCAAT 
GCCCAGCGGT CCGGCTTGAA CCAGATCCCC AACCGTCGCT TCACACTGTG GTGGTCGCCA 
ACCATTAACC GCGCCAATGT GTATGTAGGT TTCCAGGTCC AGCTGGACCT TACTGGCATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTCA TCCAGATTTT CCGTGCCCAT 
TTATGGCAGA AGATCCATGA GAGTATTGTC ATGGACTTGT GTCAGGTGTT TGACCAAGAA 
TTGGATGCGC TGGAAATTGA GACGGTGCAG AAGGAGACAA TTCACCCCCG GAAGTCCTAT 
AAGATGAACT CATCTTGTGC AGACATTCTG CTTTTTGCCT CCTACAAATG GAACGTCTCC 
CGGCCGTCTC TGCTGGCTGA CTCCAAGGAC GTGATGGACA GCACCACCAC TCAGAAGTAC 
TGGATCGACA TCCAGCTGCG CTGGGGCGAC TATGACTCCC ACGATATCGA ACGCTACGCT 
CGGGCCAAGT TTCTGGATTA CACTACCGAC AACATGAGTA TCTACCCCTC TCCAACGGGG 
GTGCTCATTG CCATAGACCT GGCATACAAT CTGCACAGCG CCTATGGAAA CTGGTTTCCA 
GGCAGCAAGC CGCTGATCCA GCAAGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCTTG 
TACGTGCTCC GAGAGCGAAT ACGCAAGGGG TTGCAGCTGT ACTCCTCCGA GCCCACAGAG 
CCTTACCTCT CCTCCCAGAA CTATGGCGAG CTCTTCTCCA ACCAGATCAT CTGGTTTGTA 
GATGACACCA ATGTCTACAG AGTCACCATT CACAAGACTT TTGAAGGGAA CTTGACTACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AATCCAAGGA CGGGGCAGCT CTTCCTGAAG 
ATCATCCACA CGTCTGTGTG GGCTGGACAG AAGCGTCTGG GTCAGTTGGC CAAGTGGAAG 
ACAGCCGAGG AAGTGGCTGC CCTGATCCGT TCTCTCCCTG TAGAAGAGCA GCCCAAACAG 
ATTATTGTGA CCAGAAAAGG CATGTTGGAC CCCTTGGAGG TGCACTTACT GGACTTCCCC 
AACATTGTGA TCAAAGGCTC GGAGCTGCAG CTGCCCTTCC AGGCCTGTCT CAAAGTGGAA 
AAATTCGGGG ACCTTATCCT CAAGGCCACC GAGCCCCAAA TGGTTCTCTT CAATCTCTAT 
GACGACTGGC TCAGAACCAT CTCATCCTAC ACGGCCTTTT CCCGTCTGAT CCTGATTCTC 
CGTGCCCTTC ATGTGAACAA TGACCGGGCC AAAGTGATCT TGAAGCCAGA CAAGACCACC 
ATCACAGAGC CGCACCACAT TTGGCCCACG CTGACCGACG AGGAGTGGAT CAAGGTTGAG 
GTGCAGCTCA AGGACTTGAT CCTGGCTGAT TACGGCAAAA AGAACAACGT CAACGTGGCC 
TCACTGACAC AATCTGAGAT CCGGGACATC ATTCTGGGCA TGGAAATCTC CGCCCCTTCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAGCAGTC CCAGCTGACG 
GCAACACAGA CCCGGACTGT CAACAAGCAC GGTGATGAGA TCATCACTTC CACCACCAGC 
AACTACGAGA CACAAACTTT TTCCTCGAAG ACCGAGTGGA GGGTCAGGGC CATCTCTGCA 
GCCAACCTGC ATCTGCGGAC CAACCACATC TACGTGTCAT CTGATGACAT CAAAGAGACA 
GGCTACACTT ACATCCTTCC CAAGAATGTC CTCAAGAAAT TCATCTGCAT TTCTGATCTT 
CGGGCCCAGA TTGCAGGCTA CCTCTATGGA GTGAGCCCCC CGGACAACCC TCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTGCCTCAG TGGGGCACCC ACCAGACGGT GCACCTGCCG 
GGCCAGCTTC CCCAACACGA GTACCTCAAG GAGATGGAGC CCCTCGGCTG GATCCACACA 
CAGCCCAACG AGTCCCCCCA GCTCTCGCCC CAGGATGTCA CCACCCATGC CAAGGTCATG 
GCCGACAACC CCTCATGGGA CGGGGAGAAG ACAATCATCA TCACCTGCAG CTTTACCCCG 
GGCTCTTGCA CCCTGACGGC CTACAAGCTG ACGCCCAGTG GTTACGAGTG GGGTCGGCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACTTGCCTT CCCACTATGA GCGTGTCCAG 
ATGCTGCTCT CTGACCGCTT CCTTGGCTTC TTCATGGTCC CCGCTCAGGC GTCCTGGAAC 
TACAATTTCA TGGGTGTTCG GCACGACCCC AACATGAAAT ACGAGCTGCA GCTGGCCAAC 
CCCAAAGAGT TCTATCACGA GGTGCACCGG CCCTCCCACT TCCTGAACTT CGCGCTGCTG 
CAGGAGGGGG AGGTGTACTC GGCCGACCGA GAGGACCTGT ACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823646.1
CDS	77..7084
Translation	MAGVFPYRGPGTPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLRTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKVMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQASWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_031967786.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii pre-mRNA processing factor 8 (PRPF8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967786.1

ORF Insert Sequence:

ATGGCCGGGG TGTTTCCCTA CAGAGGGCCG GGGACCCCCG TGCCCGGCCC TCTGGCACCG 
CTGCCCGACT ACATGTCAGA GGAGAAGCTT CAGGAAAAAG CCCGAAAATG GCAGCAGTTG 
CAGGCAAAAC GCTATGCAGA GAAGCGGAAA TTTGGATTTG TAGACGCGCA AAAGGAAGAC 
ATGCCTCCAG AGCACGTCAG GAAGATCATC CGGGACCATG GGGACATGAC GAACCGCAAG 
TTCCGTCATG ACAAGCGAGT CTACCTGGGG GCCCTGAAGT ACATGCCGCA TGCTGTCCTC 
AAGCTCTTGG AAAACATGCC CATGCCCTGG GAGCAGATCC GGGATGTGCC TGTTCTGTAC 
CACATCACTG GTGCGATTTC CTTTGTTAAT GAGATTCCTT GGGTCATCGA GCCTGTCTAC 
ATCTCTCAGT GGGGGTCCAT GTGGATCATG ATGCGTCGAG AGAAGAGGGA CAGGCGGCAC 
TTCAAGCGTA TGCGTTTTCC CCCATTTGAT GATGAGGAGC CGCCCCTTGA CTATGCTGAC 
AACATCCTCG ATGTGGAGCC TTTGGAAGCC ATTCAGCTGG AACTGGACCC TGAGGAGGAT 
GCCCCTGTGC TAGACTGGTT TTATGACCAC CAGCCCTTAA GAGACAGTAG GAAGTACGTG 
AATGGCTCTA CTTATCAGCG CTGGCAGTTC ACCCTGCCCA TGATGTCCAC TCTGTACCGC 
CTGGCCAATC AGCTCCTAAC TGATCTTGTG GATGACAATT ACTTCTATCT GTTTGACCTA 
AAGGCGTTTT TTACCTCTAA GGCCCTCAAC ATGGCTATCC CAGGGGGCCC TAAATTTGAA 
CCGCTGGTCC GAGATATCAA TCTTCAGGAT GAAGACTGGA ATGAATTTAA TGATATCAAC 
AAGATCATCA TCAGGCAGCC CATCAGAACC GAATATAAGA TTGCTTTTCC ATACTTGTAC 
AACAACCTTC CACACCACGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACAGAGG ACCCTGATCT TCCAGCCTTC TACTTTGATC CTTTGATCAA TCCTATCTCC 
CACAGACATT CTGTCAAGAG TCAAGAACCT TTGCCAGATG ATGACGAGGA GTTTGAGCTG 
CCCGAGTTTG TGGAACCTTT CCTCAAGGAC ACGCCCCTTT ACACAGATAA TACTGCCAAT 
GGCATCGCCC TTCTGTGGGC ACCTAGGCCC TTCAACCTTC GCTCTGGTCG CACCCGCCGT 
GCCCTGGACA TACCCCTGGT TAAAAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAGGTGA GGGTCTCCTA CCAAAAGCTA CTCAAATACT ACGTGCTGAA TGCCCTCAAG 
CACCGACCCC CCAAGGCCCA GAAAAAGAGG TATCTCTTCC GATCCTTTAA GGCCACCAAG 
TTCTTCCAGT CTACCAAGCT GGACTGGGTG GAGGTAGGGC TGCAGGTCTG CCGCCAGGGC 
TACAATATGC TCAACCTCCT CATCCACCGG AAGAATCTCA ACTACTTGCA TCTGGACTAT 
AACTTCAACC TAAAGCCTGT CAAGACTCTC ACCACCAAGG AAAGAAAGAA GTCTCGCTTT 
GGAAATGCCT TCCACTTGTG TCGGGAGGTG CTGCGGCTGA CCAAGCTCGT AGTGGACAGC 
CACGTTCAGT ACCGCTTGGG CAACGTGGAT GCCTTCCAGC TAGCGGACGG GCTGCAGTAT 
ATATTTGCCC ACGTGGGACA GCTGACAGGA ATGTACCGCT ACAAGTACAA GTTGATGCGG 
CAGATCCGCA TGTGCAAGGA CCTGAAGCAC CTTATCTACT ACCGCTTCAA CACGGGTCCC 
GTGGGGAAGG GCCCAGGCTG TGGTTTCTGG GCTGCTGGTT GGCGAGTCTG GTTGTTTTTC 
ATGCGTGGCA TCACCCCTCT TTTGGAACGG TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGTC ACTCAAAGGG GGTGGCAAAG ACCGTGACCA AGCAGCGAGT GGAGTCCCAC 
TTTGACTTGG AGCTCCGGGC AGCGGTGATG CATGATATCT TGGATATGAT GCCTGAGGGC 
ATCAAACAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TCCCCTGGAA GGTTCCTGGG TTGCCGACCC CCATTGAGAA CATGATCCTG 
CGCTACGTGA AGGCGAAGGC GGATTGGTGG ACCAACACCG CCCATTACAA CCGAGAGCGG 
ATCCGCCGGG GAGCCACCGT CGACAAGACC GTTTGTAAGA AGAACTTGGG TCGGCTCACA 
CGGCTCTATC TGAAGGCTGA ACAGGAACGA CAGCACAACT ACCTAAAGGA TGGTCCCTAC 
ATCACAGCAG AAGAGGCAGT GGCTGTCTAC ACCACCACTG TGCACTGGCT GGAGAGCCGC 
AGATTCTCCC CCATCCCTTT CCCCCCACTC TCCTACAAGC ATGACACCAA GCTACTCATC 
CTGGCCCTGG AGAGGCTCAA GGAGGCCTAC AGTGTGAAGT CCCGTTTGAA CCAATCCCAA 
AGAGAGGAGC TGGGCCTGAT TGAGCAAGCC TATGACAACC CGCACGAGGC TCTGTCTCGT 
ATCAAGCGTC ACTTACTCAC GCAGAGAGCC TTCAAGGAGG TGGGCATTGA GTTCATGGAC 
CTGTACAGTC ACCTGGTTCC TGTGTACGAC GTAGAACCCC TCGAGAAGAT CACAGACGCC 
TACTTGGACC AGTACCTGTG GTATGAAGCT GACAAGCGCC GGCTCTTCCC ACCCTGGATC 
AAACCTGCTG ACACGGAGCC GCCTCCCCTG CTGGTGTATA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAG GGAGAGTGCA ACGTCATGCT GGAGTCGCGC 
TTTGAGAAGA TGTACGAGAA GATCGACTTG ACGCTCCTGA ACAGGCTCCT GCGCCTCATT 
GTGGACCACA ACATTGCTGA CTATATGACG GCCAAGAACA ACGTGGTCAT CAACTACAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATTCGAGGAC TTCAGTTCGC CTCCTTCATC 
GTGCAGTACT ACGGGCTGGT GATGGACCTT CTGGTATTGG GCCTACACCG GGCCAGCGAG 
ATGGCTGGGC CTCCCCAAAT GCCCAATGAC TTCCTTAGCT TCCAAGACAT CGCCACAGAA 
GCTGCCCACC CCATCCGCCT TTTCTGTAGA TACATCGACC GCATCCACAT TTTCTTCAGG 
TTCACTGCCG ACGAGGCCCG GGACTTGATC CAGCGCTACC TCACGGAACA CCCTGACCCC 
AACAACGAGA ACATCGTGGG CTACAACAAC AAGAAGTGCT GGCCCCGCGA CGCGCGGATG 
CGCCTCATGA AGCATGACGT CAACTTGGGC CGCGCTGTGT TCTGGGACAT CAAAAACCGA 
CTGCCGCGAT CGGTAACAAC GGTGCAGTGG GAGAACAGCT TTGTGTCAGT GTACAGCAAG 
GACAACCCCA ATCTGCTCTT CAACATGTGC GGCTTCGAGT GCCGCATCCT GCCCAAATGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GATGGTGTTT GGAACCTCCA GAATGAGGTC 
ACAAAGGAGC GGACAGCTCA GTGCTTCCTG CGTGTGGATG ATGAGTCTAT GCAGCGCTTC 
CACAACCGTG TGCGTCAGAT CCTGATGGCC TCTGGCTCGA CCACCTTCAC CAAGATTGTG 
AACAAGTGGA ACACGGCTCT CATTGGGCTC ATGACGTACT TCCGTGAGGC TGTGGTGAAC 
ACCCAGGAGC TGCTGGACCT TCTGGTGAAA TGTGAGAACA AGATCCAGAC CCGAATCAAG 
ATCGGGCTCA ACTCCAAGAT GCCAAGCCGC TTCCCTCCAG TCGTGTTCTA CACCCCCAAA 
GAGCTCGGGG GGCTGGGCAT GTTGTCCATG GGCCACGTGC TCATCCCCCA GTCTGATCTC 
AGGTGGTCCA AGCAGACAGA TGTGGGCATC ACCCACTTCC GCTCCGGGAT GAGCCACGAA 
GAAGACCAGC TCATTCCTAA CTTGTACCGT TACATCCAGC CTTGGGAGAG CGAGTTCATT 
GACTCTCAAC GGGTCTGGGC CGAGTATGCG CTCAAGAGGC AGGAAGCCAT CGCTCAGAAC 
AGGCGGCTGA CCTTGGAGGA CCTGGAGGAT TCATGGGATC GAGGCATTCC CCGGATCAAT 
ACCCTGTTCC AAAAGGACCG GCACACACTG GCTTATGACA AGGGCTGGCG TGTCCGCACT 
GACTTCAAGC AATACCAGGT CCTGAAACAG AATCCTTTCT GGTGGACCCA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAATTAC CGCACAGACA TGATCCAGGC CCTGGGTGGT 
GTGGAGGGCA TCCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGT 
CTCTTCTGGG AGAAGGCCAG TGGTTTTGAG GAGTCGATGA AATGGAAGAA GCTGACCAAT 
GCCCAGCGGT CCGGCTTGAA CCAGATCCCC AACCGTCGCT TCACACTGTG GTGGTCGCCA 
ACCATTAACC GCGCCAATGT GTATGTAGGT TTCCAGGTCC AGCTGGACCT TACTGGCATC 
TTCATGCACG GCAAGATCCC CACCCTGAAG ATCTCTCTCA TCCAGATTTT CCGTGCCCAT 
TTATGGCAGA AGATCCATGA GAGTATTGTC ATGGACTTGT GTCAGGTGTT TGACCAAGAA 
TTGGATGCGC TGGAAATTGA GACGGTGCAG AAGGAGACAA TTCACCCCCG GAAGTCCTAT 
AAGATGAACT CATCTTGTGC AGACATTCTG CTTTTTGCCT CCTACAAATG GAACGTCTCC 
CGGCCGTCTC TGCTGGCTGA CTCCAAGGAC GTGATGGACA GCACCACCAC TCAGAAGTAC 
TGGATCGACA TCCAGCTGCG CTGGGGCGAC TATGACTCCC ACGATATCGA ACGCTACGCT 
CGGGCCAAGT TTCTGGATTA CACTACCGAC AACATGAGTA TCTACCCCTC TCCAACGGGG 
GTGCTCATTG CCATAGACCT GGCATACAAT CTGCACAGCG CCTATGGAAA CTGGTTTCCA 
GGCAGCAAGC CGCTGATCCA GCAAGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCTTG 
TACGTGCTCC GAGAGCGAAT ACGCAAGGGG TTGCAGCTGT ACTCCTCCGA GCCCACAGAG 
CCTTACCTCT CCTCCCAGAA CTATGGCGAG CTCTTCTCCA ACCAGATCAT CTGGTTTGTA 
GATGACACCA ATGTCTACAG AGTCACCATT CACAAGACTT TTGAAGGGAA CTTGACTACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AATCCAAGGA CGGGGCAGCT CTTCCTGAAG 
ATCATCCACA CGTCTGTGTG GGCTGGACAG AAGCGTCTGG GTCAGTTGGC CAAGTGGAAG 
ACAGCCGAGG AAGTGGCTGC CCTGATCCGT TCTCTCCCTG TAGAAGAGCA GCCCAAACAG 
ATTATTGTGA CCAGAAAAGG CATGTTGGAC CCCTTGGAGG TGCACTTACT GGACTTCCCC 
AACATTGTGA TCAAAGGCTC GGAGCTGCAG CTGCCCTTCC AGGCCTGTCT CAAAGTGGAA 
AAATTCGGGG ACCTTATCCT CAAGGCCACC GAGCCCCAAA TGGTTCTCTT CAATCTCTAT 
GACGACTGGC TCAGAACCAT CTCATCCTAC ACGGCCTTTT CCCGTCTGAT CCTGATTCTC 
CGTGCCCTTC ATGTGAACAA TGACCGGGCC AAAGTGATCT TGAAGCCAGA CAAGACCACC 
ATCACAGAGC CGCACCACAT TTGGCCCACG CTGACCGACG AGGAGTGGAT CAAGGTTGAG 
GTGCAGCTCA AGGACTTGAT CCTGGCTGAT TACGGCAAAA AGAACAACGT CAACGTGGCC 
TCACTGACAC AATCTGAGAT CCGGGACATC ATTCTGGGCA TGGAAATCTC CGCCCCTTCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAGCAGTC CCAGCTGACG 
GCAACACAGA CCCGGACTGT CAACAAGCAC GGTGATGAGA TCATCACTTC CACCACCAGC 
AACTACGAGA CACAAACTTT TTCCTCGAAG ACCGAGTGGA GGGTCAGGGC CATCTCTGCA 
GCCAACCTGC ATCTGCGGAC CAACCACATC TACGTGTCAT CTGATGACAT CAAAGAGACA 
GGCTACACTT ACATCCTTCC CAAGAATGTC CTCAAGAAAT TCATCTGCAT TTCTGATCTT 
CGGGCCCAGA TTGCAGGCTA CCTCTATGGA GTGAGCCCCC CGGACAACCC TCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTGCCTCAG TGGGGCACCC ACCAGACGGT GCACCTGCCG 
GGCCAGCTTC CCCAACACGA GTACCTCAAG GAGATGGAGC CCCTCGGCTG GATCCACACA 
CAGCCCAACG AGTCCCCCCA GCTCTCGCCC CAGGATGTCA CCACCCATGC CAAGGTCATG 
GCCGACAACC CCTCATGGGA CGGGGAGAAG ACAATCATCA TCACCTGCAG CTTTACCCCG 
GGCTCTTGCA CCCTGACGGC CTACAAGCTG ACGCCCAGTG GTTACGAGTG GGGTCGGCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACTTGCCTT CCCACTATGA GCGTGTCCAG 
ATGCTGCTCT CTGACCGCTT CCTTGGCTTC TTCATGGTCC CCGCTCAGGC GTCCTGGAAC 
TACAATTTCA TGGGTGTTCG GCACGACCCC AACATGAAAT ACGAGCTGCA GCTGGCCAAC 
CCCAAAGAGT TCTATCACGA GGTGCACCGG CCCTCCCACT TCCTGAACTT CGCGCTGCTG 
CAGGAGGGGG AGGTGTACTC GGCCGACCGA GAGGACCTGT ACGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PRPF8 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

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Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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