TTC3 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	TTC3
Entrez Gene ID	100927756
Full Name	tetratricopeptide repeat domain 3
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031959268.1	XP_031815128.1	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_031959269.1	XP_031815129.1	E3 ubiquitin-protein ligase TTC3 isoform X2
XM_012544329.1	XP_012399783.1	E3 ubiquitin-protein ligase TTC3
XM_023498851.2	XP_023354619.2	E3 ubiquitin-protein ligase TTC3 isoform X5
XM_023498852.1	XP_023354620.1	E3 ubiquitin-protein ligase TTC3 isoform X2
XM_031959270.1	XP_031815130.1	E3 ubiquitin-protein ligase TTC3 isoform X3
XM_023498851.1	XP_023354619.1	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_031959271.1	XP_031815131.1	E3 ubiquitin-protein ligase TTC3 isoform X4

Pathways from BioSystems
Gene Ontology

The following TTC3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTC3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa125127	XM_012544329.1 Latest version!	Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa151506	XM_023498851.1 Latest version!	Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa151507	XM_023498852.1 Latest version!	Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa229306	XM_031959268.1 Latest version!	Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa229307	XM_031959269.1 Latest version!	Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa229308	XM_031959270.1 Latest version!	Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa229309	XM_031959271.1 Latest version!	Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa229310	XM_023498851.2 Latest version!	Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTC3 ( XM_012544329.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012399783.1 Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), mRNA.

Price & Availability↑

CloneID	OSa125127
Clone ID Related Accession (Same CDS sequence)	XM_012544329.1
Accession Version	XM_012544329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase TTC3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831542.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTT CCATAACTGA AGCAGATTTA ATTGGCAGCA GCTACGTTCG CATTACTAAA 
CTTTGCTGTG AACAGGGTGG GGCATATCCT GCAAAATATT CACAAGTTAA GGCAAATCTA 
GAACTGGATA TCTGCAAAAT CTGGTGTAGT AAGCCACTTC ATGTCCTTCG AGATTACTGT 
GATGTCATTA AAATATACAT CTTCTGGCCA CTTCTTTTTC AACGAGAACA GACTCCTGTA 
GTGTCTCAAT TACATCCTTG TATAGATGTC AGCTCTAGTA CTTGTGAATT AAGTTTGAAG 
AGACTACAGC ATATTGAATT GCTGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTAGGC 
CATTATCGTT TTTGCGATGC TCTTTCAATG TTGGGGGAGC ATGCCTGGGC ACTAGAAGCA 
AATGAGAGAG CTCAACATCT TTGCCGAAAC TATCCAGATG GAATGAAGGA TCTTACTCAA 
CAGAACATAA AATTACAAAA ACAAGTGGAA GAATTAAGAG GTTCAAAACA TGGTAGTCAT 
CAGATAAGGA AATCGTTTTA TGGAAAAAGG TTATCCTCAG GTTATAATTC TCCAAGACCT 
TTACATTATG GTTTTCTTAA TTTTATGGAA ACTAATGAAG AAAACAAGGT ATTGAGATTA 
GCATCGAAAT ATCGTGATTA TTATCATTAT CAGAATTTGA AGGTATCTCG TGAAATTATG 
AAATTAGAAA ATAAAGACTA TCCTCCAGAT TTGCCACCAG GTCATAATCC TAAATTTAAA 
GGAAAATCAA AGAATAAAAG CGATGATGTA GGAAAACCAA GCCTACAACT GAATTTACAA 
ATAGATTTAC AAAGCTTATT GGACAAACAA TTCAGTAAAT CTTCCAGAGC TGTACAACAG 
GATTTTGCTA TATTGATAAA AATGCTGCAG TCCCTCATCG AAGAAGGTTG TACAGCTTTG 
ATGGACCAAC ATTGTCGTAG TGCAGCCAAA GCTTTTTCAC GGTTGTTAAG TGATTTAGAT 
CCCAATAAAT TGAAGCAACT GAACCTTGCC CTTATTAATT ATGTCCTAGT AATCTATGGT 
TATGCCATCT CTCTCCTTGG AATAGGAATG CCTGAGGAAT TATCAGAGGC TGAATATCAA 
TTTAAGAGAA TTATTGAGCA ATACCCCAAT GAAGGAGTTG ATTGTTTAGC CTATTTTGGA 
ATTGGAAAAG TATATTTGAA AAAAAATAGA TTTTCAGATG CACTTGGTCA TTTTCAAAAA 
TCTAAAACTA TGATTAAGCT TATTCCTGGA GTGTTGACTT GGCCTACAAC TAATGTGATT 
ATTGAAGAGT CTCAAGCTGA GAAATTGCAG TTGATATTGG AAAATCATAT TGAAGAATGC 
AAATTTCCAC CAGATCCAGA TGCAGTTTGT TGTTATCAGA AGTGCAATGG ATATTCCAAA 
ATCCAGATAT ATTTAAATGA TCCAGACTTT AAGGGTTTTA TACGAATTAA CTGCTGCCAG 
TTTTGTAGAA TAGAATTTCA TATTAGCTGT TGGAAAAAAT TAAAAACAAC AATTTTCAAT 
GATAAAAATG ACAAGGATTT TCTCCAAGGA ACATGTCTTA CTCCTGACTG TAGAGGTATT 
ATTTCGAAGA TTGTTATCTT TGGTGCAGCT GGCCATATTA AATGTGAATT TGAACATAGG 
GTCACAAAAG AAAGGTATCC ACCAAGACCT GTTATGAAGC AGAAATGTTC TAGCTTAGAG 
AAATTGAAAA TTAAAGAAGA AAAAAAAGTA AAGAGAAAAC TTCAAAAAAA AGAAGCACAA 
AAAATAGCAA AAGAGAGAAT GGAAGAGAAA ACTAGAGAAA ATAATTCATC TAAGCATGGC 
GACCATAAAG GATTTGTACA AAGTTGCCAT TTACTTGATG ATAGGATTTT GCTGTGTATA 
AAACAAAATG CCGAGCAAAT TAAATCTGGC ATCCAGGACG CTTCCAAACT TCTCAGAGAA 
TTACTTTCTT GGAAAGTAAT AAACACAGAG GATTATGCAG ATACTTATTT TAATAGCGGT 
TTTCTAAGTG AAAAAATGGA GCATCTCATT GACCATTTGG TACGGAAAAA CAATAGAGTA 
AATACAAGGA TTTTTCTGCA TGTACTGAGT GAGATTGAAG AAATAAATCC CAAACTACGT 
GACTGGATCC AGAAACTCAA TAATTTAGGT TTGGATGCTA CAAGGACGTT TTTTTCTCGT 
TATGGTGATT CTGTTAAAAA GCTTGATTTT AAATTCATAT CTATGCTCTG GGTTGAAAAA 
TATGGTCATA AACTAGATCA CATAATATCC AGTTATGAAG AGAGAAAGAT AGTTGAATAT 
TCCAATAATC AAGGATCATT GAAGGAAGCT CGTTATTTTA TATGGCTATT AGAAGAAAAT 
AGAGAAAAGT TTCCAGCATT GCATAATGCT TTAGATGAAT TCTTTGATAT AATGGATGGT 
CCTTGCACAA TATTAAAGAA GCAAGAGACT GAAGATATTT CATCTAATGG TATTAAGGTA 
AAAAACAAAA CCAGAAAAAA GAAGCACAAG GAGTCAAAGC CTGTTATCAT ACTCTCTGGA 
GTTGGTGGAG TATCACAAAA TGATGAGAAA GTTGGTACAG ATGAAAGTAC TTTACTTCAA 
TCAAATTCTT ATGACCCATT TGTAGTACCC GAATATCTTC GAGATGAAGT AGAAGAATTT 
GAAGCTCTAT ATGAAAAAAA TAGTTCAAAT AATTGTAATT ATCAGAAGTT ATTGGACAGT 
AACTTAGATC CAAAATGTGA ACCATTATAT GACTATTTTT CTCAGATCCT GGAAGAACAT 
GGTCCTTTGG AAATTGATGA CAAATTATTG GTTGGAGAAT ATGAACATTT TCCTGAAGAA 
GCTAGGAAAA TTGTGGAGGA AGCAGGAGGC TTAAAATCTT TTTTGTTAGA TTCTCTTCGT 
TTTATTATGA TAGACAATTA TATTGGATTG ATGAAAGATG CTGTTCAACT TAAGAAAAAT 
AAGACCACTG GAACTGATGG GGAAGAAAGT ACTAGTTTGG ATGAGAAAGA CTCAATGATT 
TTCTCTCCAC GCTTTCAGAA AAATGCTTCC CAGCTCAAAC TACAGTTGAA TCCAGCAGCT 
AAAGAATTTA AACCAGTAAC TTGTAATTCT TTCATACCAG CACCTAAAAA TAAAAAAACA 
AATAAGACTG CAAAGTATGT AACAACAGGA TATGTGTCAT ATATTCCTTT TAATACAGTT 
TTTTCTAATC AAGGATTTGA CACCAGTAAC ACATCAGTGT CTTATGCAAA TATGTTGCCA 
CAGTCATCTC AATGCATTAG TTTATATACA ACTGTATCTG ATATATCTTC TGATTATCCA 
AAAAAAAGAA AACTACCATT GGTGTCAGCT GTACCTCCTA TGACAGATAA TGACAAAAGA 
GATAATTTTG ACTCTGGCAA TTCCGAGGCA TGTAATGTAA AATCTGAAAG ACCAATGCTT 
GAAAAAGTAT GTTCCTCTGG TAAACCTAAT TGTGAAAAAA AGATCCGTCA GAATACTCAG 
AGAACAACTC TGAAGGCAGA AATATGTAAA AATAATACTG CCAAAGATGC ATGCCTTGGA 
AGTAATGATC ATTGTGATCT TGGCACTAGT AAACTAGAAC GAGAGATGAA AAATACGACT 
ATCATCAAAA ATACTCCATG TATACGAATG GTTGCCATAC AGGTAAAAAC TGACTTAATG 
CATCAGGAGG TTAATACACT GCCTTTTGGT CCATTTGAAA CCCAACAAGG TGATATTTTA 
CGAATGGAAA AGGAACATCA GGTGTTACAA GAGCAACTTA AAGAAGCAAG GGAAAAATAT 
GAACAGTTAA AATGCAGAAG TACAGAGGAA ACCAGAGAGG TGGAAGAAAA ACTGAAAAGA 
ACAATAGAAG AAAATAAGAT ATCCAAGACT GAATTGAATT GGTTCCATCA AGATTTGGAA 
ATAGAAGTGA AAAAATGGCA ACAAGAAAAA AAGGAAAATC AAGAAAAATT GAAAACTTTA 
AGAAATAAAG TTAAAAAGCT TAATGATACT CATGAAATAT ATTCAAGAAA TAATGAAGAA 
AAGGATCAAC AGTATGCATC ACACTTGGAT GAATTTCTCA AAATCAGTAA TAAATTTGCA 
AATGAAAAAT TGAAAATGGA GGAACTTATA AAAAAGGGAA AAGAGAATTA TGAGGAAACC 
ATGAAAAGAG CTGTGGCTGC AGAGGTGTCC ATACTTGAAA ATTGGAAGAC TACTGATGTG 
AGTAAGTTAC AGAACACTGC ATCAAAAGCA GAAATGTATG TGAAGAATCT AAAGTTAATG 
AACAGTGATT CTGCATCATC TCTTAATTTG GAATCAGAGA TTACTCACTG GGAATCATTT 
ATTTCAGAAA TAAAAAAAGA AATTGAGAAG GCAGAGTTTG AGTTTGAGGA TCGAATTCAG 
AAAGTTAAAG AAGGTTCTCA ACTGGATAAA CTTTCTAAAG TAAAAGTTCC TGAATTTCCA 
TTACCTACGG ATAATTTATT AATTCAAGAC AAGCCTGCTG ATATTAATGA TCCAGCCTCT 
GTGACATATT CTGCACAAGT TCATTCTGAC TTATTTTCTG AATATGATGA TCAAAGTCCA 
GTGATTATCC CAGCTGACTT ATTAACTGGA AGCCAAGCCC TGACACTTCA GAACACAAAT 
CTACATTCTG GCAGTGACTG CTCAGAGGAG ATAACTTCTG TACTTTTACA AGCATCTTAT 
ACTGATCCAT GTCAGGTCAC CCAGCCATCA CCGATTGTTA AGAGTTCTGA AAAGGATACT 
CAAATTAATC AAAGTTCCAA AGGTGTTTCA CAGACACATG CCAGTACTCC AAACCAGCCA 
CCTTTTAACA ATGTGATTGA GCATCTGTCA ACCATATTCC CAAATTACAG CAGTGTTGAG 
CTTTTGAATT TTATCCAAAA AGTACAAAAT CAAGACAGAA CTACACATGC AAGTTTGGAG 
TCTGATGAAA TTGTCAGAAG AGTAACAGAA CTTATTCTAG ATGAGCAGAA TAAAAAACTG 
GCAGTTCAAG GAACAGACAT GAGTGGTGCA GCTGAATCGT CTGATCTTAG ACATTCAGCA 
GAAAATGTAT CAGTATCTAG TCCCTCCATA GTCCCAGCTT CAGAAACACT TGCCAGCAAA 
CCCTGGGTAG TTGTTGGAGG ACCCTCCAAG TCAAAGTGGC AAAAATCACA TGATTCCTCT 
GTCTCCAGTG ATGATCCCTG CATAATATGC TATGAAGAGC TAAACCAGGA ACATGTATGT 
GAGATGGATT GTGGGCACCA GTTTCATAAA TGGTGCATTG AGCAGTGGCT TAAAGAACAA 
AGTACATGCC CAACATGCCG TGAATATGTC CTGTTAGCAG AAGAATTTCC TGCACTTTCT 
GGATCAGGCC GGCCTGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012399783.1
CDS	110..5530
Translation	METSITEADLIGSSYVRITKLCCEQGGAYPAKYSQVKANLELDICKIWCSKPLHVLRDYCDVIKIYIFWPLLFQREQTPVVSQLHPCIDVSSSTCELSLKRLQHIELLEDIVDLAKKVVGHYRFCDALSMLGEHAWALEANERAQHLCRNYPDGMKDLTQQNIKLQKQVEELRGSKHGSHQIRKSFYGKRLSSGYNSPRPLHYGFLNFMETNEENKVLRLASKYRDYYHYQNLKVSREIMKLENKDYPPDLPPGHNPKFKGKSKNKSDDVGKPSLQLNLQIDLQSLLDKQFSKSSRAVQQDFAILIKMLQSLIEEGCTALMDQHCRSAAKAFSRLLSDLDPNKLKQLNLALINYVLVIYGYAISLLGIGMPEELSEAEYQFKRIIEQYPNEGVDCLAYFGIGKVYLKKNRFSDALGHFQKSKTMIKLIPGVLTWPTTNVIIEESQAEKLQLILENHIEECKFPPDPDAVCCYQKCNGYSKIQIYLNDPDFKGFIRINCCQFCRIEFHISCWKKLKTTIFNDKNDKDFLQGTCLTPDCRGIISKIVIFGAAGHIKCEFEHRVTKERYPPRPVMKQKCSSLEKLKIKEEKKVKRKLQKKEAQKIAKERMEEKTRENNSSKHGDHKGFVQSCHLLDDRILLCIKQNAEQIKSGIQDASKLLRELLSWKVINTEDYADTYFNSGFLSEKMEHLIDHLVRKNNRVNTRIFLHVLSEIEEINPKLRDWIQKLNNLGLDATRTFFSRYGDSVKKLDFKFISMLWVEKYGHKLDHIISSYEERKIVEYSNNQGSLKEARYFIWLLEENREKFPALHNALDEFFDIMDGPCTILKKQETEDISSNGIKVKNKTRKKKHKESKPVIILSGVGGVSQNDEKVGTDESTLLQSNSYDPFVVPEYLRDEVEEFEALYEKNSSNNCNYQKLLDSNLDPKCEPLYDYFSQILEEHGPLEIDDKLLVGEYEHFPEEARKIVEEAGGLKSFLLDSLRFIMIDNYIGLMKDAVQLKKNKTTGTDGEESTSLDEKDSMIFSPRFQKNASQLKLQLNPAAKEFKPVTCNSFIPAPKNKKTNKTAKYVTTGYVSYIPFNTVFSNQGFDTSNTSVSYANMLPQSSQCISLYTTVSDISSDYPKKRKLPLVSAVPPMTDNDKRDNFDSGNSEACNVKSERPMLEKVCSSGKPNCEKKIRQNTQRTTLKAEICKNNTAKDACLGSNDHCDLGTSKLEREMKNTTIIKNTPCIRMVAIQVKTDLMHQEVNTLPFGPFETQQGDILRMEKEHQVLQEQLKEAREKYEQLKCRSTEETREVEEKLKRTIEENKISKTELNWFHQDLEIEVKKWQQEKKENQEKLKTLRNKVKKLNDTHEIYSRNNEEKDQQYASHLDEFLKISNKFANEKLKMEELIKKGKENYEETMKRAVAAEVSILENWKTTDVSKLQNTASKAEMYVKNLKLMNSDSASSLNLESEITHWESFISEIKKEIEKAEFEFEDRIQKVKEGSQLDKLSKVKVPEFPLPTDNLLIQDKPADINDPASVTYSAQVHSDLFSEYDDQSPVIIPADLLTGSQALTLQNTNLHSGSDCSEEITSVLLQASYTDPCQVTQPSPIVKSSEKDTQINQSSKGVSQTHASTPNQPPFNNVIEHLSTIFPNYSSVELLNFIQKVQNQDRTTHASLESDEIVRRVTELILDEQNKKLAVQGTDMSGAAESSDLRHSAENVSVSSPSIVPASETLASKPWVVVGGPSKSKWQKSHDSSVSSDDPCIICYEELNQEHVCEMDCGHQFHKWCIEQWLKEQSTCPTCREYVLLAEEFPALSGSGRPA

Target ORF information:

RefSeq Version	XM_012544329.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012544329.1

ORF Insert Sequence:

ATGGAGACTT CCATAACTGA AGCAGATTTA ATTGGCAGCA GCTACGTTCG CATTACTAAA 
CTTTGCTGTG AACAGGGTGG GGCATATCCT GCAAAATATT CACAAGTTAA GGCAAATCTA 
GAACTGGATA TCTGCAAAAT CTGGTGTAGT AAGCCACTTC ATGTCCTTCG AGATTACTGT 
GATGTCATTA AAATATACAT CTTCTGGCCA CTTCTTTTTC AACGAGAACA GACTCCTGTA 
GTGTCTCAAT TACATCCTTG TATAGATGTC AGCTCTAGTA CTTGTGAATT AAGTTTGAAG 
AGACTACAGC ATATTGAATT GCTGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTAGGC 
CATTATCGTT TTTGCGATGC TCTTTCAATG TTGGGGGAGC ATGCCTGGGC ACTAGAAGCA 
AATGAGAGAG CTCAACATCT TTGCCGAAAC TATCCAGATG GAATGAAGGA TCTTACTCAA 
CAGAACATAA AATTACAAAA ACAAGTGGAA GAATTAAGAG GTTCAAAACA TGGTAGTCAT 
CAGATAAGGA AATCGTTTTA TGGAAAAAGG TTATCCTCAG GTTATAATTC TCCAAGACCT 
TTACATTATG GTTTTCTTAA TTTTATGGAA ACTAATGAAG AAAACAAGGT ATTGAGATTA 
GCATCGAAAT ATCGTGATTA TTATCATTAT CAGAATTTGA AGGTATCTCG TGAAATTATG 
AAATTAGAAA ATAAAGACTA TCCTCCAGAT TTGCCACCAG GTCATAATCC TAAATTTAAA 
GGAAAATCAA AGAATAAAAG CGATGATGTA GGAAAACCAA GCCTACAACT GAATTTACAA 
ATAGATTTAC AAAGCTTATT GGACAAACAA TTCAGTAAAT CTTCCAGAGC TGTACAACAG 
GATTTTGCTA TATTGATAAA AATGCTGCAG TCCCTCATCG AAGAAGGTTG TACAGCTTTG 
ATGGACCAAC ATTGTCGTAG TGCAGCCAAA GCTTTTTCAC GGTTGTTAAG TGATTTAGAT 
CCCAATAAAT TGAAGCAACT GAACCTTGCC CTTATTAATT ATGTCCTAGT AATCTATGGT 
TATGCCATCT CTCTCCTTGG AATAGGAATG CCTGAGGAAT TATCAGAGGC TGAATATCAA 
TTTAAGAGAA TTATTGAGCA ATACCCCAAT GAAGGAGTTG ATTGTTTAGC CTATTTTGGA 
ATTGGAAAAG TATATTTGAA AAAAAATAGA TTTTCAGATG CACTTGGTCA TTTTCAAAAA 
TCTAAAACTA TGATTAAGCT TATTCCTGGA GTGTTGACTT GGCCTACAAC TAATGTGATT 
ATTGAAGAGT CTCAAGCTGA GAAATTGCAG TTGATATTGG AAAATCATAT TGAAGAATGC 
AAATTTCCAC CAGATCCAGA TGCAGTTTGT TGTTATCAGA AGTGCAATGG ATATTCCAAA 
ATCCAGATAT ATTTAAATGA TCCAGACTTT AAGGGTTTTA TACGAATTAA CTGCTGCCAG 
TTTTGTAGAA TAGAATTTCA TATTAGCTGT TGGAAAAAAT TAAAAACAAC AATTTTCAAT 
GATAAAAATG ACAAGGATTT TCTCCAAGGA ACATGTCTTA CTCCTGACTG TAGAGGTATT 
ATTTCGAAGA TTGTTATCTT TGGTGCAGCT GGCCATATTA AATGTGAATT TGAACATAGG 
GTCACAAAAG AAAGGTATCC ACCAAGACCT GTTATGAAGC AGAAATGTTC TAGCTTAGAG 
AAATTGAAAA TTAAAGAAGA AAAAAAAGTA AAGAGAAAAC TTCAAAAAAA AGAAGCACAA 
AAAATAGCAA AAGAGAGAAT GGAAGAGAAA ACTAGAGAAA ATAATTCATC TAAGCATGGC 
GACCATAAAG GATTTGTACA AAGTTGCCAT TTACTTGATG ATAGGATTTT GCTGTGTATA 
AAACAAAATG CCGAGCAAAT TAAATCTGGC ATCCAGGACG CTTCCAAACT TCTCAGAGAA 
TTACTTTCTT GGAAAGTAAT AAACACAGAG GATTATGCAG ATACTTATTT TAATAGCGGT 
TTTCTAAGTG AAAAAATGGA GCATCTCATT GACCATTTGG TACGGAAAAA CAATAGAGTA 
AATACAAGGA TTTTTCTGCA TGTACTGAGT GAGATTGAAG AAATAAATCC CAAACTACGT 
GACTGGATCC AGAAACTCAA TAATTTAGGT TTGGATGCTA CAAGGACGTT TTTTTCTCGT 
TATGGTGATT CTGTTAAAAA GCTTGATTTT AAATTCATAT CTATGCTCTG GGTTGAAAAA 
TATGGTCATA AACTAGATCA CATAATATCC AGTTATGAAG AGAGAAAGAT AGTTGAATAT 
TCCAATAATC AAGGATCATT GAAGGAAGCT CGTTATTTTA TATGGCTATT AGAAGAAAAT 
AGAGAAAAGT TTCCAGCATT GCATAATGCT TTAGATGAAT TCTTTGATAT AATGGATGGT 
CCTTGCACAA TATTAAAGAA GCAAGAGACT GAAGATATTT CATCTAATGG TATTAAGGTA 
AAAAACAAAA CCAGAAAAAA GAAGCACAAG GAGTCAAAGC CTGTTATCAT ACTCTCTGGA 
GTTGGTGGAG TATCACAAAA TGATGAGAAA GTTGGTACAG ATGAAAGTAC TTTACTTCAA 
TCAAATTCTT ATGACCCATT TGTAGTACCC GAATATCTTC GAGATGAAGT AGAAGAATTT 
GAAGCTCTAT ATGAAAAAAA TAGTTCAAAT AATTGTAATT ATCAGAAGTT ATTGGACAGT 
AACTTAGATC CAAAATGTGA ACCATTATAT GACTATTTTT CTCAGATCCT GGAAGAACAT 
GGTCCTTTGG AAATTGATGA CAAATTATTG GTTGGAGAAT ATGAACATTT TCCTGAAGAA 
GCTAGGAAAA TTGTGGAGGA AGCAGGAGGC TTAAAATCTT TTTTGTTAGA TTCTCTTCGT 
TTTATTATGA TAGACAATTA TATTGGATTG ATGAAAGATG CTGTTCAACT TAAGAAAAAT 
AAGACCACTG GAACTGATGG GGAAGAAAGT ACTAGTTTGG ATGAGAAAGA CTCAATGATT 
TTCTCTCCAC GCTTTCAGAA AAATGCTTCC CAGCTCAAAC TACAGTTGAA TCCAGCAGCT 
AAAGAATTTA AACCAGTAAC TTGTAATTCT TTCATACCAG CACCTAAAAA TAAAAAAACA 
AATAAGACTG CAAAGTATGT AACAACAGGA TATGTGTCAT ATATTCCTTT TAATACAGTT 
TTTTCTAATC AAGGATTTGA CACCAGTAAC ACATCAGTGT CTTATGCAAA TATGTTGCCA 
CAGTCATCTC AATGCATTAG TTTATATACA ACTGTATCTG ATATATCTTC TGATTATCCA 
AAAAAAAGAA AACTACCATT GGTGTCAGCT GTACCTCCTA TGACAGATAA TGACAAAAGA 
GATAATTTTG ACTCTGGCAA TTCCGAGGCA TGTAATGTAA AATCTGAAAG ACCAATGCTT 
GAAAAAGTAT GTTCCTCTGG TAAACCTAAT TGTGAAAAAA AGATCCGTCA GAATACTCAG 
AGAACAACTC TGAAGGCAGA AATATGTAAA AATAATACTG CCAAAGATGC ATGCCTTGGA 
AGTAATGATC ATTGTGATCT TGGCACTAGT AAACTAGAAC GAGAGATGAA AAATACGACT 
ATCATCAAAA ATACTCCATG TATACGAATG GTTGCCATAC AGGTAAAAAC TGACTTAATG 
CATCAGGAGG TTAATACACT GCCTTTTGGT CCATTTGAAA CCCAACAAGG TGATATTTTA 
CGAATGGAAA AGGAACATCA GGTGTTACAA GAGCAACTTA AAGAAGCAAG GGAAAAATAT 
GAACAGTTAA AATGCAGAAG TACAGAGGAA ACCAGAGAGG TGGAAGAAAA ACTGAAAAGA 
ACAATAGAAG AAAATAAGAT ATCCAAGACT GAATTGAATT GGTTCCATCA AGATTTGGAA 
ATAGAAGTGA AAAAATGGCA ACAAGAAAAA AAGGAAAATC AAGAAAAATT GAAAACTTTA 
AGAAATAAAG TTAAAAAGCT TAATGATACT CATGAAATAT ATTCAAGAAA TAATGAAGAA 
AAGGATCAAC AGTATGCATC ACACTTGGAT GAATTTCTCA AAATCAGTAA TAAATTTGCA 
AATGAAAAAT TGAAAATGGA GGAACTTATA AAAAAGGGAA AAGAGAATTA TGAGGAAACC 
ATGAAAAGAG CTGTGGCTGC AGAGGTGTCC ATACTTGAAA ATTGGAAGAC TACTGATGTG 
AGTAAGTTAC AGAACACTGC ATCAAAAGCA GAAATGTATG TGAAGAATCT AAAGTTAATG 
AACAGTGATT CTGCATCATC TCTTAATTTG GAATCAGAGA TTACTCACTG GGAATCATTT 
ATTTCAGAAA TAAAAAAAGA AATTGAGAAG GCAGAGTTTG AGTTTGAGGA TCGAATTCAG 
AAAGTTAAAG AAGGTTCTCA ACTGGATAAA CTTTCTAAAG TAAAAGTTCC TGAATTTCCA 
TTACCTACGG ATAATTTATT AATTCAAGAC AAGCCTGCTG ATATTAATGA TCCAGCCTCT 
GTGACATATT CTGCACAAGT TCATTCTGAC TTATTTTCTG AATATGATGA TCAAAGTCCA 
GTGATTATCC CAGCTGACTT ATTAACTGGA AGCCAAGCCC TGACACTTCA GAACACAAAT 
CTACATTCTG GCAGTGACTG CTCAGAGGAG ATAACTTCTG TACTTTTACA AGCATCTTAT 
ACTGATCCAT GTCAGGTCAC CCAGCCATCA CCGATTGTTA AGAGTTCTGA AAAGGATACT 
CAAATTAATC AAAGTTCCAA AGGTGTTTCA CAGACACATG CCAGTACTCC AAACCAGCCA 
CCTTTTAACA ATGTGATTGA GCATCTGTCA ACCATATTCC CAAATTACAG CAGTGTTGAG 
CTTTTGAATT TTATCCAAAA AGTACAAAAT CAAGACAGAA CTACACATGC AAGTTTGGAG 
TCTGATGAAA TTGTCAGAAG AGTAACAGAA CTTATTCTAG ATGAGCAGAA TAAAAAACTG 
GCAGTTCAAG GAACAGACAT GAGTGGTGCA GCTGAATCGT CTGATCTTAG ACATTCAGCA 
GAAAATGTAT CAGTATCTAG TCCCTCCATA GTCCCAGCTT CAGAAACACT TGCCAGCAAA 
CCCTGGGTAG TTGTTGGAGG ACCCTCCAAG TCAAAGTGGC AAAAATCACA TGATTCCTCT 
GTCTCCAGTG ATGATCCCTG CATAATATGC TATGAAGAGC TAAACCAGGA ACATGTATGT 
GAGATGGATT GTGGGCACCA GTTTCATAAA TGGTGCATTG AGCAGTGGCT TAAAGAACAA 
AGTACATGCC CAACATGCCG TGAATATGTC CTGTTAGCAG AAGAATTTCC TGCACTTTCT 
GGATCAGGCC GGCCTGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_023498851.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023354619.1 Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa151506
Clone ID Related Accession (Same CDS sequence)	XM_023498851.1
Accession Version	XM_023498851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831542.1) and transcript sequence (GEDN01140258.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 274 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAGAGAGC TCAACATCTT TGCCAATGAA GAATTCTCAG AAGAGAGGTT TGATACAGCT 
ATCACATATT ATACCAGAGC AATTGAATTT AGACCTGAAA ACCATCTTCT TTATAGTAAC 
CGAGCTCTTT GTTTCCTTAG GACTGGTCAA TTCAAGAGTG CACTTGGTGA TGGAAAGAGG 
GCCACTATTC TGAAATACAA TTGGCCAAAG GGCCATTATC GTTTTTGCGA TGCTCTTTCA 
ATGTTGGGGG AGCATGCCTG GGCACTAGAA GCAAATGAGA GAGCTCAACA TCTTTGCCGA 
AACTATCCAG ATGGAATGAA GGATCTTACT CAACAGAACA TAAAATTACA AAAACAAGTG 
GAAGAATTAA GAGGTTCAAA ACATGGTAGT CATCAGATAA GGAAATCGTT TTATGGAAAA 
AGGTTATCCT CAGGTTATAA TTCTCCAAGA CCTTTACATT ATGGTTTTCT TAATTTTATG 
GAAACTAATG AAGAAAACAA GGTATTGAGA TTAGCATCGA AATATCGTGA TTATTATCAT 
TATCAGAATT TGAAGGTATC TCGTGAAATT ATGAAATTAG AAAATAAAGA CTATCCTCCA 
GATTTGCCAC CAGGTCATAA TCCTAAATTT AAAGGAAAAT CAAAGAATAA AAGCGATGAT 
GTAGGAAAAC CAAGCCTACA ACTGAATTTA CAAATAGATT TACAAAGCTT ATTGGACAAA 
CAATTCAGTA AATCTTCCAG AGCTGTACAA CAGGATTTTG CTATATTGAT AAAAATGCTG 
CAGTCCCTCA TCGAAGAAGG TTGTACAGCT TTGATGGACC AACATTGTCG TAGTGCAGCC 
AAAGCTTTTT CACGGTTGTT AAGTGATTTA GATCCCAATA AATTGAAGCA ACTGAACCTT 
GCCCTTATTA ATTATGTCCT AGTAATCTAT GGTTATGCCA TCTCTCTCCT TGGAATAGGA 
ATGCCTGAGG AATTATCAGA GGCTGAATAT CAATTTAAGA GAATTATTGA GCAATACCCC 
AATGAAGGAG TTGATTGTTT AGCCTATTTT GGAATTGGAA AAGTATATTT GAAAAAAAAT 
AGATTTTCAG ATGCACTTGG TCATTTTCAA AAATCTAAAA CTATGATTAA GCTTATTCCT 
GGAGTGTTGA CTTGGCCTAC AACTAATGTG ATTATTGAAG AGTCTCAAGC TGAGAAATTG 
CAGTTGATAT TGGAAAATCA TATTGAAGAA TGCAAATTTC CACCAGATCC AGATGCAGTT 
TGTTGTTATC AGAAGTGCAA TGGATATTCC AAAATCCAGA TATATTTAAA TGATCCAGAC 
TTTAAGGGTT TTATACGAAT TAACTGCTGC CAGTTTTGTA GAATAGAATT TCATATTAGC 
TGTTGGAAAA AATTAAAAAC AACAATTTTC AATGATAAAA ATGACAAGGA TTTTCTCCAA 
GGAACATGTC TTACTCCTGA CTGTAGAGGT ATTATTTCGA AGATTGTTAT CTTTGGTGCA 
GCTGGCCATA TTAAATGTGA ATTTGAACAT AGGGTCACAA AAGAAAGGTA TCCACCAAGA 
CCTGTTATGA AGCAGAAATG TTCTAGCTTA GAGAAATTGA AAATTAAAGA AGAAAAAAAA 
GTAAAGAGAA AACTTCAAAA AAAAGAAGCA CAAAAAATAG CAAAAGAGAG AATGGAAGAG 
AAAACTAGAG AAAATAATTC ATCTAAGCAT GGCGACCATA AAGGATTTGT ACAAAGTTGC 
CATTTACTTG ATGATAGGAT TTTGCTGTGT ATAAAACAAA ATGCCGAGCA AATTAAATCT 
GGCATCCAGG ACGCTTCCAA ACTTCTCAGA GAATTACTTT CTTGGAAAGT AATAAACACA 
GAGGATTATG CAGATACTTA TTTTAATAGC GGTTTTCTAA GTGAAAAAAT GGAGCATCTC 
ATTGACCATT TGGTACGGAA AAACAATAGA GTAAATACAA GGATTTTTCT GCATGTACTG 
AGTGAGATTG AAGAAATAAA TCCCAAACTA CGTGACTGGA TCCAGAAACT CAATAATTTA 
GGTTTGGATG CTACAAGGAC GTTTTTTTCT CGTTATGGTG ATTCTGTTAA AAAGCTTGAT 
TTTAAATTCA TATCTATGCT CTGGGTTGAA AAATATGGTC ATAAACTAGA TCACATAATA 
TCCAGTTATG AAGAGAGAAA GATAGTTGAA TATTCCAATA ATCAAGGATC ATTGAAGGAA 
GCTCGTTATT TTATATGGCT ATTAGAAGAA AATAGAGAAA AGTTTCCAGC ATTGCATAAT 
GCTTTAGATG AATTCTTTGA TATAATGGAT GGTCCTTGCA CAATATTAAA GAAGCAAGAG 
ACTGAAGATA TTTCATCTAA TGGTATTAAG GTAAAAAACA AAACCAGAAA AAAGAAGCAC 
AAGGAGTCAA AGCCTGTTAT CATACTCTCT GGAGTTGGTG GAGTATCACA AAATGATGAG 
AAAGTTGGTA CAGATGAAAG TACTTTACTT CAATCAAATT CTTATGACCC ATTTGTAGTA 
CCCGAATATC TTCGAGATGA AGTAGAAGAA TTTGAAGCTC TATATGAAAA AAATAGTTCA 
AATAATTGTA ATTATCAGAA GTTATTGGAC AGTAACTTAG ATCCAAAATG TGAACCATTA 
TATGACTATT TTTCTCAGAT CCTGGAAGAA CATGGTCCTT TGGAAATTGA TGACAAATTA 
TTGGTTGGAG AATATGAACA TTTTCCTGAA GAAGCTAGGA AAATTGTGGA GGAAGCAGGA 
GGCTTAAAAT CTTTTTTGTT AGATTCTCTT CGTTTTATTA TGATAGACAA TTATATTGGA 
TTGATGAAAG ATGCTGTTCA ACTTAAGAAA AATAAGACCA CTGGAACTGA TGGGGAAGAA 
AGTACTAGTT TGGATGAGAA AGACTCAATG ATTTTCTCTC CACGCTTTCA GAAAAATGCT 
TCCCAGCTCA AACTACAGTT GAATCCAGCA GCTAAAGAAT TTAAACCAGT AACTTGTAAT 
TCTTTCATAC CAGCACCTAA AAATAAAAAA ACAAATAAGA CTGCAAAGTA TGTAACAACA 
GGATATGTGT CATATATTCC TTTTAATACA GTTTTTTCTA ATCAAGGATT TGACACCAGT 
AACACATCAG TGTCTTATGC AAATATGTTG CCACAGTCAT CTCAATGCAT TAGTTTATAT 
ACAACTGTAT CTGATATATC TTCTGATTAT CCAAAAAAAA GAAAACTACC ATTGGTGTCA 
GCTGTACCTC CTATGACAGA TAATGACAAA AGAGATAATT TTGACTCTGG CAATTCCGAG 
GCATGTAATG TAAAATCTGA AAGACCAATG CTTGAAAAAG TATGTTCCTC TGGTAAACCT 
AATTGTGAAA AAAAGATCCG TCAGAATACT CAGAGAACAA CTCTGAAGGC AGAAATATGT 
AAAAATAATA CTGCCAAAGA TGCATGCCTT GGAAGTAATG ATCATTGTGA TCTTGGCACT 
AGTAAACTAG AACGAGAGAT GAAAAATACG ACTATCATCA AAAATACTCC ATGTATACGA 
ATGGTTGCCA TACAGGTAAA AACTGACTTA ATGCATCAGG AGGTTAATAC ACTGCCTTTT 
GGTCCATTTG AAACCCAACA AGGTGATATT TTACGAATGG AAAAGGAACA TCAGGTGTTA 
CAAGAGCAAC TTAAAGAAGC AAGGGAAAAA TATGAACAGT TAAAATGCAG AAGTACAGAG 
GAAACCAGAG AGGTGGAAGA AAAACTGAAA AGAACAATAG AAGAAAATAA GATATCCAAG 
ACTGAATTGA ATTGGTTCCA TCAAGATTTG GAAATAGAAG TGAAAAAATG GCAACAAGAA 
AAAAAGGAAA ATCAAGAAAA ATTGAAAACT TTAAGAAATA AAGTTAAAAA GCTTAATGAT 
ACTCATGAAA TATATTCAAG AAATAATGAA GAAAAGGATC AACAGTATGC ATCACACTTG 
GATGAATTTC TCAAAATCAG TAATAAATTT GCAAATGAAA AATTGAAAAT GGAGGAACTT 
ATAAAAAAGG GAAAAGAGAA TTATGAGGAA ACCATGAAAA GAGCTGTGGC TGCAGAGGTG 
TCCATACTTG AAAATTGGAA GACTACTGAT GTGAGTAAGT TACAGAACAC TGCATCAAAA 
GCAGAAATGT ATGTGAAGAA TCTAAAGTTA ATGAACAGTG ATTCTGCATC ATCTCTTAAT 
TTGGAATCAG AGATTACTCA CTGGGAATCA TTTATTTCAG AAATAAAAAA AGAAATTGAG 
AAGGCAGAGT TTGAGTTTGA GGATCGAATT CAGAAAGTTA AAGAAGGTTC TCAACTGGAT 
AAACTTTCTA AAGTAAAAGT TCCTGAATTT CCATTACCTA CGGATAATTT ATTAATTCAA 
GACAAGCCTG CTGATATTAA TGATCCAGCC TCTGTGACAT ATTCTGCACA AGTTCATTCT 
GACTTATTTT CTGAATATGA TGATCAAAGT CCAGTGATTA TCCCAGCTGA CTTATTAACT 
GGAAGCCAAG CCCTGACACT TCAGAACACA AATCTACATT CTGGCAGTGA CTGCTCAGAG 
GAGATAACTT CTGTACTTTT ACAAGCATCT TATACTGATC CATGTCAGGT CACCCAGCCA 
TCACCGATTG TTAAGAGTTC TGAAAAGGAT ACTCAAATTA ATCAAAGTTC CAAAGGTGTT 
TCACAGACAC ATGCCAGTAC TCCAAACCAG CCACCTTTTA ACAATGTGAT TGAGCATCTG 
TCAACCATAT TCCCAAATTA CAGCAGTGTT GAGCTTTTGA ATTTTATCCA AAAAGTACAA 
AATCAAGACA GAACTACACA TGCAAGTTTG GAGTCTGATG AAATTGTCAG AAGAGTAACA 
GAACTTATTC TAGATGAGCA GAATAAAAAA CTGGCAGTTC AAGGAACAGA CATGAGTGGT 
GCAGCTGAAT CGTCTGATCT TAGACATTCA GCAGAAAATG TATCAGTATC TAGTCCCTCC 
ATAGTCCCAG CTTCAGAAAC ACTTGCCAGC AAACCCTGGG TAGTTGTTGG AGGACCCTCC 
AAGTCAAAGT GGCAAAAATC ACATGATTCC TCTGTCTCCA GTGATGATCC CTGCATAATA 
TGCTATGAAG AGCTAAACCA GGAACATGTA TGTGAGATGG ATTGTGGGCA CCAGTTTCAT 
AAATGGTGCA TTGAGCAGTG GCTTAAAGAA CAAAGTACAT GCCCAACATG CCGTGAATAT 
GTCCTGTTAG CAGAAGAATT TCCTGCACTT TCTGGATCAG GCCGGCCTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023354619.1
CDS	65..5338
Translation	MRELNIFANEEFSEERFDTAITYYTRAIEFRPENHLLYSNRALCFLRTGQFKSALGDGKRATILKYNWPKGHYRFCDALSMLGEHAWALEANERAQHLCRNYPDGMKDLTQQNIKLQKQVEELRGSKHGSHQIRKSFYGKRLSSGYNSPRPLHYGFLNFMETNEENKVLRLASKYRDYYHYQNLKVSREIMKLENKDYPPDLPPGHNPKFKGKSKNKSDDVGKPSLQLNLQIDLQSLLDKQFSKSSRAVQQDFAILIKMLQSLIEEGCTALMDQHCRSAAKAFSRLLSDLDPNKLKQLNLALINYVLVIYGYAISLLGIGMPEELSEAEYQFKRIIEQYPNEGVDCLAYFGIGKVYLKKNRFSDALGHFQKSKTMIKLIPGVLTWPTTNVIIEESQAEKLQLILENHIEECKFPPDPDAVCCYQKCNGYSKIQIYLNDPDFKGFIRINCCQFCRIEFHISCWKKLKTTIFNDKNDKDFLQGTCLTPDCRGIISKIVIFGAAGHIKCEFEHRVTKERYPPRPVMKQKCSSLEKLKIKEEKKVKRKLQKKEAQKIAKERMEEKTRENNSSKHGDHKGFVQSCHLLDDRILLCIKQNAEQIKSGIQDASKLLRELLSWKVINTEDYADTYFNSGFLSEKMEHLIDHLVRKNNRVNTRIFLHVLSEIEEINPKLRDWIQKLNNLGLDATRTFFSRYGDSVKKLDFKFISMLWVEKYGHKLDHIISSYEERKIVEYSNNQGSLKEARYFIWLLEENREKFPALHNALDEFFDIMDGPCTILKKQETEDISSNGIKVKNKTRKKKHKESKPVIILSGVGGVSQNDEKVGTDESTLLQSNSYDPFVVPEYLRDEVEEFEALYEKNSSNNCNYQKLLDSNLDPKCEPLYDYFSQILEEHGPLEIDDKLLVGEYEHFPEEARKIVEEAGGLKSFLLDSLRFIMIDNYIGLMKDAVQLKKNKTTGTDGEESTSLDEKDSMIFSPRFQKNASQLKLQLNPAAKEFKPVTCNSFIPAPKNKKTNKTAKYVTTGYVSYIPFNTVFSNQGFDTSNTSVSYANMLPQSSQCISLYTTVSDISSDYPKKRKLPLVSAVPPMTDNDKRDNFDSGNSEACNVKSERPMLEKVCSSGKPNCEKKIRQNTQRTTLKAEICKNNTAKDACLGSNDHCDLGTSKLEREMKNTTIIKNTPCIRMVAIQVKTDLMHQEVNTLPFGPFETQQGDILRMEKEHQVLQEQLKEAREKYEQLKCRSTEETREVEEKLKRTIEENKISKTELNWFHQDLEIEVKKWQQEKKENQEKLKTLRNKVKKLNDTHEIYSRNNEEKDQQYASHLDEFLKISNKFANEKLKMEELIKKGKENYEETMKRAVAAEVSILENWKTTDVSKLQNTASKAEMYVKNLKLMNSDSASSLNLESEITHWESFISEIKKEIEKAEFEFEDRIQKVKEGSQLDKLSKVKVPEFPLPTDNLLIQDKPADINDPASVTYSAQVHSDLFSEYDDQSPVIIPADLLTGSQALTLQNTNLHSGSDCSEEITSVLLQASYTDPCQVTQPSPIVKSSEKDTQINQSSKGVSQTHASTPNQPPFNNVIEHLSTIFPNYSSVELLNFIQKVQNQDRTTHASLESDEIVRRVTELILDEQNKKLAVQGTDMSGAAESSDLRHSAENVSVSSPSIVPASETLASKPWVVVGGPSKSKWQKSHDSSVSSDDPCIICYEELNQEHVCEMDCGHQFHKWCIEQWLKEQSTCPTCREYVLLAEEFPALSGSGRPA

Target ORF information:

RefSeq Version	XM_023498851.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023498851.1

ORF Insert Sequence:

ATGAGAGAGC TCAACATCTT TGCCAATGAA GAATTCTCAG AAGAGAGGTT TGATACAGCT 
ATCACATATT ATACCAGAGC AATTGAATTT AGACCTGAAA ACCATCTTCT TTATAGTAAC 
CGAGCTCTTT GTTTCCTTAG GACTGGTCAA TTCAAGAGTG CACTTGGTGA TGGAAAGAGG 
GCCACTATTC TGAAATACAA TTGGCCAAAG GGCCATTATC GTTTTTGCGA TGCTCTTTCA 
ATGTTGGGGG AGCATGCCTG GGCACTAGAA GCAAATGAGA GAGCTCAACA TCTTTGCCGA 
AACTATCCAG ATGGAATGAA GGATCTTACT CAACAGAACA TAAAATTACA AAAACAAGTG 
GAAGAATTAA GAGGTTCAAA ACATGGTAGT CATCAGATAA GGAAATCGTT TTATGGAAAA 
AGGTTATCCT CAGGTTATAA TTCTCCAAGA CCTTTACATT ATGGTTTTCT TAATTTTATG 
GAAACTAATG AAGAAAACAA GGTATTGAGA TTAGCATCGA AATATCGTGA TTATTATCAT 
TATCAGAATT TGAAGGTATC TCGTGAAATT ATGAAATTAG AAAATAAAGA CTATCCTCCA 
GATTTGCCAC CAGGTCATAA TCCTAAATTT AAAGGAAAAT CAAAGAATAA AAGCGATGAT 
GTAGGAAAAC CAAGCCTACA ACTGAATTTA CAAATAGATT TACAAAGCTT ATTGGACAAA 
CAATTCAGTA AATCTTCCAG AGCTGTACAA CAGGATTTTG CTATATTGAT AAAAATGCTG 
CAGTCCCTCA TCGAAGAAGG TTGTACAGCT TTGATGGACC AACATTGTCG TAGTGCAGCC 
AAAGCTTTTT CACGGTTGTT AAGTGATTTA GATCCCAATA AATTGAAGCA ACTGAACCTT 
GCCCTTATTA ATTATGTCCT AGTAATCTAT GGTTATGCCA TCTCTCTCCT TGGAATAGGA 
ATGCCTGAGG AATTATCAGA GGCTGAATAT CAATTTAAGA GAATTATTGA GCAATACCCC 
AATGAAGGAG TTGATTGTTT AGCCTATTTT GGAATTGGAA AAGTATATTT GAAAAAAAAT 
AGATTTTCAG ATGCACTTGG TCATTTTCAA AAATCTAAAA CTATGATTAA GCTTATTCCT 
GGAGTGTTGA CTTGGCCTAC AACTAATGTG ATTATTGAAG AGTCTCAAGC TGAGAAATTG 
CAGTTGATAT TGGAAAATCA TATTGAAGAA TGCAAATTTC CACCAGATCC AGATGCAGTT 
TGTTGTTATC AGAAGTGCAA TGGATATTCC AAAATCCAGA TATATTTAAA TGATCCAGAC 
TTTAAGGGTT TTATACGAAT TAACTGCTGC CAGTTTTGTA GAATAGAATT TCATATTAGC 
TGTTGGAAAA AATTAAAAAC AACAATTTTC AATGATAAAA ATGACAAGGA TTTTCTCCAA 
GGAACATGTC TTACTCCTGA CTGTAGAGGT ATTATTTCGA AGATTGTTAT CTTTGGTGCA 
GCTGGCCATA TTAAATGTGA ATTTGAACAT AGGGTCACAA AAGAAAGGTA TCCACCAAGA 
CCTGTTATGA AGCAGAAATG TTCTAGCTTA GAGAAATTGA AAATTAAAGA AGAAAAAAAA 
GTAAAGAGAA AACTTCAAAA AAAAGAAGCA CAAAAAATAG CAAAAGAGAG AATGGAAGAG 
AAAACTAGAG AAAATAATTC ATCTAAGCAT GGCGACCATA AAGGATTTGT ACAAAGTTGC 
CATTTACTTG ATGATAGGAT TTTGCTGTGT ATAAAACAAA ATGCCGAGCA AATTAAATCT 
GGCATCCAGG ACGCTTCCAA ACTTCTCAGA GAATTACTTT CTTGGAAAGT AATAAACACA 
GAGGATTATG CAGATACTTA TTTTAATAGC GGTTTTCTAA GTGAAAAAAT GGAGCATCTC 
ATTGACCATT TGGTACGGAA AAACAATAGA GTAAATACAA GGATTTTTCT GCATGTACTG 
AGTGAGATTG AAGAAATAAA TCCCAAACTA CGTGACTGGA TCCAGAAACT CAATAATTTA 
GGTTTGGATG CTACAAGGAC GTTTTTTTCT CGTTATGGTG ATTCTGTTAA AAAGCTTGAT 
TTTAAATTCA TATCTATGCT CTGGGTTGAA AAATATGGTC ATAAACTAGA TCACATAATA 
TCCAGTTATG AAGAGAGAAA GATAGTTGAA TATTCCAATA ATCAAGGATC ATTGAAGGAA 
GCTCGTTATT TTATATGGCT ATTAGAAGAA AATAGAGAAA AGTTTCCAGC ATTGCATAAT 
GCTTTAGATG AATTCTTTGA TATAATGGAT GGTCCTTGCA CAATATTAAA GAAGCAAGAG 
ACTGAAGATA TTTCATCTAA TGGTATTAAG GTAAAAAACA AAACCAGAAA AAAGAAGCAC 
AAGGAGTCAA AGCCTGTTAT CATACTCTCT GGAGTTGGTG GAGTATCACA AAATGATGAG 
AAAGTTGGTA CAGATGAAAG TACTTTACTT CAATCAAATT CTTATGACCC ATTTGTAGTA 
CCCGAATATC TTCGAGATGA AGTAGAAGAA TTTGAAGCTC TATATGAAAA AAATAGTTCA 
AATAATTGTA ATTATCAGAA GTTATTGGAC AGTAACTTAG ATCCAAAATG TGAACCATTA 
TATGACTATT TTTCTCAGAT CCTGGAAGAA CATGGTCCTT TGGAAATTGA TGACAAATTA 
TTGGTTGGAG AATATGAACA TTTTCCTGAA GAAGCTAGGA AAATTGTGGA GGAAGCAGGA 
GGCTTAAAAT CTTTTTTGTT AGATTCTCTT CGTTTTATTA TGATAGACAA TTATATTGGA 
TTGATGAAAG ATGCTGTTCA ACTTAAGAAA AATAAGACCA CTGGAACTGA TGGGGAAGAA 
AGTACTAGTT TGGATGAGAA AGACTCAATG ATTTTCTCTC CACGCTTTCA GAAAAATGCT 
TCCCAGCTCA AACTACAGTT GAATCCAGCA GCTAAAGAAT TTAAACCAGT AACTTGTAAT 
TCTTTCATAC CAGCACCTAA AAATAAAAAA ACAAATAAGA CTGCAAAGTA TGTAACAACA 
GGATATGTGT CATATATTCC TTTTAATACA GTTTTTTCTA ATCAAGGATT TGACACCAGT 
AACACATCAG TGTCTTATGC AAATATGTTG CCACAGTCAT CTCAATGCAT TAGTTTATAT 
ACAACTGTAT CTGATATATC TTCTGATTAT CCAAAAAAAA GAAAACTACC ATTGGTGTCA 
GCTGTACCTC CTATGACAGA TAATGACAAA AGAGATAATT TTGACTCTGG CAATTCCGAG 
GCATGTAATG TAAAATCTGA AAGACCAATG CTTGAAAAAG TATGTTCCTC TGGTAAACCT 
AATTGTGAAA AAAAGATCCG TCAGAATACT CAGAGAACAA CTCTGAAGGC AGAAATATGT 
AAAAATAATA CTGCCAAAGA TGCATGCCTT GGAAGTAATG ATCATTGTGA TCTTGGCACT 
AGTAAACTAG AACGAGAGAT GAAAAATACG ACTATCATCA AAAATACTCC ATGTATACGA 
ATGGTTGCCA TACAGGTAAA AACTGACTTA ATGCATCAGG AGGTTAATAC ACTGCCTTTT 
GGTCCATTTG AAACCCAACA AGGTGATATT TTACGAATGG AAAAGGAACA TCAGGTGTTA 
CAAGAGCAAC TTAAAGAAGC AAGGGAAAAA TATGAACAGT TAAAATGCAG AAGTACAGAG 
GAAACCAGAG AGGTGGAAGA AAAACTGAAA AGAACAATAG AAGAAAATAA GATATCCAAG 
ACTGAATTGA ATTGGTTCCA TCAAGATTTG GAAATAGAAG TGAAAAAATG GCAACAAGAA 
AAAAAGGAAA ATCAAGAAAA ATTGAAAACT TTAAGAAATA AAGTTAAAAA GCTTAATGAT 
ACTCATGAAA TATATTCAAG AAATAATGAA GAAAAGGATC AACAGTATGC ATCACACTTG 
GATGAATTTC TCAAAATCAG TAATAAATTT GCAAATGAAA AATTGAAAAT GGAGGAACTT 
ATAAAAAAGG GAAAAGAGAA TTATGAGGAA ACCATGAAAA GAGCTGTGGC TGCAGAGGTG 
TCCATACTTG AAAATTGGAA GACTACTGAT GTGAGTAAGT TACAGAACAC TGCATCAAAA 
GCAGAAATGT ATGTGAAGAA TCTAAAGTTA ATGAACAGTG ATTCTGCATC ATCTCTTAAT 
TTGGAATCAG AGATTACTCA CTGGGAATCA TTTATTTCAG AAATAAAAAA AGAAATTGAG 
AAGGCAGAGT TTGAGTTTGA GGATCGAATT CAGAAAGTTA AAGAAGGTTC TCAACTGGAT 
AAACTTTCTA AAGTAAAAGT TCCTGAATTT CCATTACCTA CGGATAATTT ATTAATTCAA 
GACAAGCCTG CTGATATTAA TGATCCAGCC TCTGTGACAT ATTCTGCACA AGTTCATTCT 
GACTTATTTT CTGAATATGA TGATCAAAGT CCAGTGATTA TCCCAGCTGA CTTATTAACT 
GGAAGCCAAG CCCTGACACT TCAGAACACA AATCTACATT CTGGCAGTGA CTGCTCAGAG 
GAGATAACTT CTGTACTTTT ACAAGCATCT TATACTGATC CATGTCAGGT CACCCAGCCA 
TCACCGATTG TTAAGAGTTC TGAAAAGGAT ACTCAAATTA ATCAAAGTTC CAAAGGTGTT 
TCACAGACAC ATGCCAGTAC TCCAAACCAG CCACCTTTTA ACAATGTGAT TGAGCATCTG 
TCAACCATAT TCCCAAATTA CAGCAGTGTT GAGCTTTTGA ATTTTATCCA AAAAGTACAA 
AATCAAGACA GAACTACACA TGCAAGTTTG GAGTCTGATG AAATTGTCAG AAGAGTAACA 
GAACTTATTC TAGATGAGCA GAATAAAAAA CTGGCAGTTC AAGGAACAGA CATGAGTGGT 
GCAGCTGAAT CGTCTGATCT TAGACATTCA GCAGAAAATG TATCAGTATC TAGTCCCTCC 
ATAGTCCCAG CTTCAGAAAC ACTTGCCAGC AAACCCTGGG TAGTTGTTGG AGGACCCTCC 
AAGTCAAAGT GGCAAAAATC ACATGATTCC TCTGTCTCCA GTGATGATCC CTGCATAATA 
TGCTATGAAG AGCTAAACCA GGAACATGTA TGTGAGATGG ATTGTGGGCA CCAGTTTCAT 
AAATGGTGCA TTGAGCAGTG GCTTAAAGAA CAAAGTACAT GCCCAACATG CCGTGAATAT 
GTCCTGTTAG CAGAAGAATT TCCTGCACTT TCTGGATCAG GCCGGCCTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_023498852.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023354620.1 Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa151507
Clone ID Related Accession (Same CDS sequence)	XM_023498852.1
Accession Version	XM_023498852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase TTC3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831542.1) and transcript sequence (GEDN01140258.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 274 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAGAGAGC TCAACATCTT TGCCAATGAA GAATTCTCAG AAGAGAGGTT TGATACAGCT 
ATCACATATT ATACCAGAGC AATTGAATTT AGACCTGAAA ACCATCTTCT TTATAGTAAC 
CGAGCTCTTT GTTTCCTTAG GACTGGTCAA TTCAAGAGTG CACTTGGTGA TGGAAAGAGG 
GCCACTATTC TGAAATACAA TTGGCCAAAG GGCCATTATC GTTTTTGCGA TGCTCTTTCA 
ATGTTGGGGG AGCATGCCTG GGCACTAGAA GCAAATGAGA GAGCTCAACA TCTTTGCCGA 
AACTATCCAG ATGGAATGAA GGATCTTACT CAACAGAACA TAAAATTACA AAAACAAGTG 
GAAGAATTAA GAGGTTCAAA ACATGGTAGT CATCAGATAA GGAAATCGTT TTATGGAAAA 
AGGTTATCCT CAGGTTATAA TTCTCCAAGA CCTTTACATT ATGGTTTTCT TAATTTTATG 
GAAACTAATG AAGAAAACAA GGTATTGAGA TTAGCATCGA AATATCGTGA TTATTATCAT 
TATCAGAATT TGAAGGTATC TCGTGAAATT ATGAAATTAG AAAATAAAGA CTATCCTCCA 
GATTTGCCAC CAGGTCATAA TCCTAAATTT AAAGGAAAAT CAAAGAATAA AAGCGATGAT 
GTAGGAAAAC CAAGCCTACA ACTGAATTTA CAAATAGATT TACAAAGCTT ATTGGACAAA 
CAATTCAGTA AATCTTCCAG AGCTGTACAA CAGGATTTTG CTATATTGAT AAAAATGCTG 
CAGTCCCTCA TCGAAGAAGG TTGTACAGCT TTGATGGACC AACATTGTCG TAGTGCAGCC 
AAAGCTTTTT CACGGTTGTT AAGTGATTTA GATCCCAATA AATTGAAGCA ACTGAACCTT 
GCCCTTATTA ATTATGTCCT AGTAATCTAT GGTTATGCCA TCTCTCTCCT TGGAATAGGA 
ATGCCTGAGG AATTATCAGA GGCTGAATAT CAATTTAAGA GAATTATTGA GCAATACCCC 
AATGAAGGAG TTGATTGTTT AGCCTATTTT GGAATTGGAA AAGTATATTT GAAAAAAAAT 
AGATTTTCAG ATGCACTTGG TCATTTTCAA AAATCTAAAA CTATGATTAA GCTTATTCCT 
GGAGTGTTGA CTTGGCCTAC AACTAATGTG ATTATTGAAG AGTCTCAAGC TGAGAAATTG 
CAGTTGATAT TGGAAAATCA TATTGAAGAA TGCAAATTTC CACCAGATCC AGATGCAGTT 
TGTTGTTATC AGAAGTGCAA TGGATATTCC AAAATCCAGA TATATTTAAA TGATCCAGAC 
TTTAAGGGTT TTATACGAAT TAACTGCTGC CAGTTTTGTA GAATAGAATT TCATATTAGC 
TGTTGGAAAA AATTAAAAAC AACAATTTTC AATGATAAAA ATGACAAGGA TTTTCTCCAA 
GGAACATGTC TTACTCCTGA CTGTAGAGGT ATTATTTCGA AGATTGTTAT CTTTGGTGCA 
GCTGGCCATA TTAAATGTGA ATTTGAACAT AGGGTCACAA AAGAAAGGTA TCCACCAAGA 
CCTGTTATGA AGCAGAAATG TTCTAGCTTA GAGAAATTGA AAATTAAAGA AGAAAAAAAA 
GTAAAGAGAA AACTTCAAAA AAAAGAAGCA CAAAAAATAG CAAAAGAGAG AATGGAAGAG 
AAAACTAGAG AAAATAATTC ATCTAAGCAT GGCGACCATA AAGGATTTGT ACAAAGTTGC 
CATTTACTTG ATGATAGGAT TTTGCTGTGT ATAAAACAAA ATGCCGAGCA AATTAAATCT 
GGCATCCAGG ACGCTTCCAA ACTTCTCAGA GAATTACTTT CTTGGAAAGT AATAAACACA 
GAGGATTATG CAGATACTTA TTTTAATAGC GGTTTTCTAA GTGAAAAAAT GGAGCATCTC 
ATTGACCATT TGGTACGGAA AAACAATAGA GTAAATACAA GGATTTTTCT GCATGTACTG 
AGTGAGATTG AAGAAATAAA TCCCAAACTA CGTGACTGGA TCCAGAAACT CAATAATTTA 
GGTTTGGATG CTACAAGGAC GTTTTTTTCT CGTTATGGTG ATTCTGTTAA AAAGCTTGAT 
TTTAAATTCA TATCTATGCT CTGGGTTGAA AAATATGGTC ATAAACTAGA TCACATAATA 
TCCAGTTATG AAGAGAGAAA GATAGTTGAA TATTCCAATA ATCAAGGATC ATTGAAGGAA 
GCTCGTTATT TTATATGGCT ATTAGAAGAA AATAGAGAAA AGTTTCCAGC ATTGCATAAT 
GCTTTAGATG AATTCTTTGA TATAATGGAT GGTCCTTGCA CAATATTAAA GAAGCAAGAG 
ACTGAAGATA TTTCATCTAA TGGTATTAAG GTAAAAAACA AAACCAGAAA AAAGAAGCAC 
AAGGAGTCAA AGCCTGTTAT CATACTCTCT GGAGTTGGTG GAGTATCACA AAATGATGAG 
AAAGTTGGTA CAGATGAAAG TACTTTACTT CAATCAAATT CTTATGACCC ATTTGTAGTA 
CCCGAATATC TTCGAGATGA AGTAGAAGAA TTTGAAGCTC TATATGAAAA AAATAGTTCA 
AATAATTGTA ATTATCAGAA GTTATTGGAC AGTAACTTAG ATCCAAAATG TGAACCATTA 
TATGACTATT TTTCTCAGAT CCTGGAAGAA CATGGTCCTT TGGAAATTGA TGACAAATTA 
TTGGTTGGAG AATATGAACA TTTTCCTGAA GAAGCTAGGA AAATTGTGGA GGAAGCAGGA 
GGCTTAAAAT CTTTTTTGTT AGATTCTCTT CGTTTTATTA TGATAGACAA TTATATTGGA 
TTGATGAAAG ATGCTGTTCA ACTTAAGAAA AATAAGACCA CTGGAACTGA TGGGGAAGAA 
AGTACTAGTT TGGATGAGAA AGACTCAATG ATTTTCTCTC CACGCTTTCA GAAAAATGCT 
TCCCAGCTCA AACTACAGTT GAATCCAGCA GCTAAAGAAT TTAAACCAGT AACTTGTAAT 
TCTTTCATAC CAGCACCTAA AAATAAAAAA ACAAATAAGA CTGCAAAGTA TGTAACAACA 
GGATATGTGT CATATATTCC TTTTAATACA GTTTTTTCTA ATCAAGGATT TGACACCAGT 
AACACATCAG TGTCTTATGC AAATATGTTG CCACAGTCAT CTCAATGCAT TAGTTTATAT 
ACAACTGTAT CTGATATATC TTCTGATTAT CCAAAAAAAA GAAAACTACC ATTGGTGTCA 
GCTGTACCTC CTATGACAGA TAATGACAAA AGAGATAATT TTGACTCTGG CAATTCCGAG 
GCATGTAATG TAAAATCTGA AAGACCAATG CTTGAAAAAG TATGTTCCTC TGGTAAACCT 
AATTGTGAAA AAAAGATCCG TCAGAATACT CAGAGAACAA CTCTGAAGGC AGAAATATGT 
AAAAATAATA CTGCCAAAGA TGCATGCCTT GGAAGTAATG ATCATTGTGA TCTTGGCACT 
AGTAAACTAG AACGAGAGAT GAAAAATACG ACTATCATCA AAAATACTCC ATGTATACGA 
ATGGTTGCCA TACAGGTAAA AACTGACTTA ATGCATCAGG AGGTTAATAC ACTGCCTTTT 
GGTCCATTTG AAACCCAACA AGGTGATATT TTACGAATGG AAAAGGAACA TCAGGTGTTA 
CAAGAGCAAC TTAAAGAAGC AAGGGAAAAA TATGAACAGT TAAAATGCAG AAGTACAGAG 
GAAACCAGAG AGGTGGAAGA AAAACTGAAA AGAACAATAG AAGAAAATAA GATATCCAAG 
ACTGAATTGA ATTGGTTCCA TCAAGATTTG GAAATAGAAG TGAAAAAATG GCAACAAGAA 
AAAAAGGAAA ATCAAGAAAA ATTGAAAACT TTAAGAAATA AAGTTAAAAA GCTTAATGAT 
ACTCATGAAA TATATTCAAG AAATAATGAA GAAAAGGATC AACAGTATGC ATCACACTTG 
GATGAATTTC TCAAAATCAG TAATAAATTT GCAAATGAAA AATTGAAAAT GGAGGAACTT 
ATAAAAAAGG GAAAAGAGAA TTATGAGGAA ACCATGAAAA GAGCTGTGGC TGCAGAGGTG 
TCCATACTTG AAAATTGGAA GACTACTGAT GTGAGTAAGT TACAGAACAC TGCATCAAAA 
GCAGAAATGT ATGTGAAGAA TCTAAAGTTA ATGAACAGTG ATTCTGCATC ATCTCTTAAT 
TTGGAATCAG AGATTACTCA CTGGGAATCA TTTATTTCAG AAATAAAAAA AGAAATTGAG 
AAGGCAGAGT TTGAGTTTGA GGATCGAATT CAGAAAGTTA AAGAAGGTTC TCAACTGGAT 
AAACTTTCTA AAGTAAAAGT TCCTGAATTT CCATTACCTA CGGATAATTT ATTAATTCAA 
GACAAGCCTG CTGATATTAA TGATCCAGCC TCTGTGACAT ATTCTGCACA AGTTCATTCT 
GACTTATTTT CTGAATATGA TGATCAAAGT CCAGTGATTA TCCCAGCTGA CTTATTAACT 
GGAAGCCAAG CCCTGACACT TCAGAACACA AATCTACATT CTGGCAGTGA CTGCTCAGAG 
GAGATAACTT CTGTACTTTT ACAAGCATCT TATACTGATC CATGTCAGGT CACCCAGCCA 
TCACCGATTG TTAAGAGTTC TGAAAAGGAT ACTCAAATTA ATCAAAGTTC CAAAGGTGTT 
TCACAGACAC ATGCCAGTAC TCCAAACCAG CCACCTTTTA ACAATGTGAT TGAGCATCTG 
TCAACCATAT TCCCAAATTA CAGCAGTGTT GAGCTTTTGA ATTTTATCCA AAAAGTACAA 
AATCAAGACA GAACTACACA TGCAAGTTTG GAGTCTGATG AAATTGTCAG AAGAGTAACA 
GAACTTATTC TAGATGAGCA GAATAAAAAA CTGGCAGTTC AAGGAACAGA CATGAGTGGT 
GCAGCTGAAT CGTCTGATCT TAGACATTCA GCAGAAAATG TATCAGTATC TAGTCCCTCC 
ATAGTCCCAG CTTCAGAAAC ACTTGCCAGC AAACCCTGGG TAGTTGTTGG AGGACCCTCC 
AAGTCAAAGT GGCAAAAATC ACATGATTCC TCTGTCTCCA GTGATGATCC CTGCATAATA 
TGCTATGAAG AGCTAAACCA GGAACATGTA TGTGAGATGG ATTGTGGGCA CCAGTTTCAT 
AAATGGGCAA CAGCTACTGA CCCTGAAATC CCACTGCTGA TCAACACAGA AGATCTGACC 
TACTCTGTTT CTGACTCGGG CCAGTTTACC CCTCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023354620.1
CDS	65..5323
Translation	MRELNIFANEEFSEERFDTAITYYTRAIEFRPENHLLYSNRALCFLRTGQFKSALGDGKRATILKYNWPKGHYRFCDALSMLGEHAWALEANERAQHLCRNYPDGMKDLTQQNIKLQKQVEELRGSKHGSHQIRKSFYGKRLSSGYNSPRPLHYGFLNFMETNEENKVLRLASKYRDYYHYQNLKVSREIMKLENKDYPPDLPPGHNPKFKGKSKNKSDDVGKPSLQLNLQIDLQSLLDKQFSKSSRAVQQDFAILIKMLQSLIEEGCTALMDQHCRSAAKAFSRLLSDLDPNKLKQLNLALINYVLVIYGYAISLLGIGMPEELSEAEYQFKRIIEQYPNEGVDCLAYFGIGKVYLKKNRFSDALGHFQKSKTMIKLIPGVLTWPTTNVIIEESQAEKLQLILENHIEECKFPPDPDAVCCYQKCNGYSKIQIYLNDPDFKGFIRINCCQFCRIEFHISCWKKLKTTIFNDKNDKDFLQGTCLTPDCRGIISKIVIFGAAGHIKCEFEHRVTKERYPPRPVMKQKCSSLEKLKIKEEKKVKRKLQKKEAQKIAKERMEEKTRENNSSKHGDHKGFVQSCHLLDDRILLCIKQNAEQIKSGIQDASKLLRELLSWKVINTEDYADTYFNSGFLSEKMEHLIDHLVRKNNRVNTRIFLHVLSEIEEINPKLRDWIQKLNNLGLDATRTFFSRYGDSVKKLDFKFISMLWVEKYGHKLDHIISSYEERKIVEYSNNQGSLKEARYFIWLLEENREKFPALHNALDEFFDIMDGPCTILKKQETEDISSNGIKVKNKTRKKKHKESKPVIILSGVGGVSQNDEKVGTDESTLLQSNSYDPFVVPEYLRDEVEEFEALYEKNSSNNCNYQKLLDSNLDPKCEPLYDYFSQILEEHGPLEIDDKLLVGEYEHFPEEARKIVEEAGGLKSFLLDSLRFIMIDNYIGLMKDAVQLKKNKTTGTDGEESTSLDEKDSMIFSPRFQKNASQLKLQLNPAAKEFKPVTCNSFIPAPKNKKTNKTAKYVTTGYVSYIPFNTVFSNQGFDTSNTSVSYANMLPQSSQCISLYTTVSDISSDYPKKRKLPLVSAVPPMTDNDKRDNFDSGNSEACNVKSERPMLEKVCSSGKPNCEKKIRQNTQRTTLKAEICKNNTAKDACLGSNDHCDLGTSKLEREMKNTTIIKNTPCIRMVAIQVKTDLMHQEVNTLPFGPFETQQGDILRMEKEHQVLQEQLKEAREKYEQLKCRSTEETREVEEKLKRTIEENKISKTELNWFHQDLEIEVKKWQQEKKENQEKLKTLRNKVKKLNDTHEIYSRNNEEKDQQYASHLDEFLKISNKFANEKLKMEELIKKGKENYEETMKRAVAAEVSILENWKTTDVSKLQNTASKAEMYVKNLKLMNSDSASSLNLESEITHWESFISEIKKEIEKAEFEFEDRIQKVKEGSQLDKLSKVKVPEFPLPTDNLLIQDKPADINDPASVTYSAQVHSDLFSEYDDQSPVIIPADLLTGSQALTLQNTNLHSGSDCSEEITSVLLQASYTDPCQVTQPSPIVKSSEKDTQINQSSKGVSQTHASTPNQPPFNNVIEHLSTIFPNYSSVELLNFIQKVQNQDRTTHASLESDEIVRRVTELILDEQNKKLAVQGTDMSGAAESSDLRHSAENVSVSSPSIVPASETLASKPWVVVGGPSKSKWQKSHDSSVSSDDPCIICYEELNQEHVCEMDCGHQFHKWATATDPEIPLLINTEDLTYSVSDSGQFTPP

Target ORF information:

RefSeq Version	XM_023498852.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023498852.1

ORF Insert Sequence:

ATGAGAGAGC TCAACATCTT TGCCAATGAA GAATTCTCAG AAGAGAGGTT TGATACAGCT 
ATCACATATT ATACCAGAGC AATTGAATTT AGACCTGAAA ACCATCTTCT TTATAGTAAC 
CGAGCTCTTT GTTTCCTTAG GACTGGTCAA TTCAAGAGTG CACTTGGTGA TGGAAAGAGG 
GCCACTATTC TGAAATACAA TTGGCCAAAG GGCCATTATC GTTTTTGCGA TGCTCTTTCA 
ATGTTGGGGG AGCATGCCTG GGCACTAGAA GCAAATGAGA GAGCTCAACA TCTTTGCCGA 
AACTATCCAG ATGGAATGAA GGATCTTACT CAACAGAACA TAAAATTACA AAAACAAGTG 
GAAGAATTAA GAGGTTCAAA ACATGGTAGT CATCAGATAA GGAAATCGTT TTATGGAAAA 
AGGTTATCCT CAGGTTATAA TTCTCCAAGA CCTTTACATT ATGGTTTTCT TAATTTTATG 
GAAACTAATG AAGAAAACAA GGTATTGAGA TTAGCATCGA AATATCGTGA TTATTATCAT 
TATCAGAATT TGAAGGTATC TCGTGAAATT ATGAAATTAG AAAATAAAGA CTATCCTCCA 
GATTTGCCAC CAGGTCATAA TCCTAAATTT AAAGGAAAAT CAAAGAATAA AAGCGATGAT 
GTAGGAAAAC CAAGCCTACA ACTGAATTTA CAAATAGATT TACAAAGCTT ATTGGACAAA 
CAATTCAGTA AATCTTCCAG AGCTGTACAA CAGGATTTTG CTATATTGAT AAAAATGCTG 
CAGTCCCTCA TCGAAGAAGG TTGTACAGCT TTGATGGACC AACATTGTCG TAGTGCAGCC 
AAAGCTTTTT CACGGTTGTT AAGTGATTTA GATCCCAATA AATTGAAGCA ACTGAACCTT 
GCCCTTATTA ATTATGTCCT AGTAATCTAT GGTTATGCCA TCTCTCTCCT TGGAATAGGA 
ATGCCTGAGG AATTATCAGA GGCTGAATAT CAATTTAAGA GAATTATTGA GCAATACCCC 
AATGAAGGAG TTGATTGTTT AGCCTATTTT GGAATTGGAA AAGTATATTT GAAAAAAAAT 
AGATTTTCAG ATGCACTTGG TCATTTTCAA AAATCTAAAA CTATGATTAA GCTTATTCCT 
GGAGTGTTGA CTTGGCCTAC AACTAATGTG ATTATTGAAG AGTCTCAAGC TGAGAAATTG 
CAGTTGATAT TGGAAAATCA TATTGAAGAA TGCAAATTTC CACCAGATCC AGATGCAGTT 
TGTTGTTATC AGAAGTGCAA TGGATATTCC AAAATCCAGA TATATTTAAA TGATCCAGAC 
TTTAAGGGTT TTATACGAAT TAACTGCTGC CAGTTTTGTA GAATAGAATT TCATATTAGC 
TGTTGGAAAA AATTAAAAAC AACAATTTTC AATGATAAAA ATGACAAGGA TTTTCTCCAA 
GGAACATGTC TTACTCCTGA CTGTAGAGGT ATTATTTCGA AGATTGTTAT CTTTGGTGCA 
GCTGGCCATA TTAAATGTGA ATTTGAACAT AGGGTCACAA AAGAAAGGTA TCCACCAAGA 
CCTGTTATGA AGCAGAAATG TTCTAGCTTA GAGAAATTGA AAATTAAAGA AGAAAAAAAA 
GTAAAGAGAA AACTTCAAAA AAAAGAAGCA CAAAAAATAG CAAAAGAGAG AATGGAAGAG 
AAAACTAGAG AAAATAATTC ATCTAAGCAT GGCGACCATA AAGGATTTGT ACAAAGTTGC 
CATTTACTTG ATGATAGGAT TTTGCTGTGT ATAAAACAAA ATGCCGAGCA AATTAAATCT 
GGCATCCAGG ACGCTTCCAA ACTTCTCAGA GAATTACTTT CTTGGAAAGT AATAAACACA 
GAGGATTATG CAGATACTTA TTTTAATAGC GGTTTTCTAA GTGAAAAAAT GGAGCATCTC 
ATTGACCATT TGGTACGGAA AAACAATAGA GTAAATACAA GGATTTTTCT GCATGTACTG 
AGTGAGATTG AAGAAATAAA TCCCAAACTA CGTGACTGGA TCCAGAAACT CAATAATTTA 
GGTTTGGATG CTACAAGGAC GTTTTTTTCT CGTTATGGTG ATTCTGTTAA AAAGCTTGAT 
TTTAAATTCA TATCTATGCT CTGGGTTGAA AAATATGGTC ATAAACTAGA TCACATAATA 
TCCAGTTATG AAGAGAGAAA GATAGTTGAA TATTCCAATA ATCAAGGATC ATTGAAGGAA 
GCTCGTTATT TTATATGGCT ATTAGAAGAA AATAGAGAAA AGTTTCCAGC ATTGCATAAT 
GCTTTAGATG AATTCTTTGA TATAATGGAT GGTCCTTGCA CAATATTAAA GAAGCAAGAG 
ACTGAAGATA TTTCATCTAA TGGTATTAAG GTAAAAAACA AAACCAGAAA AAAGAAGCAC 
AAGGAGTCAA AGCCTGTTAT CATACTCTCT GGAGTTGGTG GAGTATCACA AAATGATGAG 
AAAGTTGGTA CAGATGAAAG TACTTTACTT CAATCAAATT CTTATGACCC ATTTGTAGTA 
CCCGAATATC TTCGAGATGA AGTAGAAGAA TTTGAAGCTC TATATGAAAA AAATAGTTCA 
AATAATTGTA ATTATCAGAA GTTATTGGAC AGTAACTTAG ATCCAAAATG TGAACCATTA 
TATGACTATT TTTCTCAGAT CCTGGAAGAA CATGGTCCTT TGGAAATTGA TGACAAATTA 
TTGGTTGGAG AATATGAACA TTTTCCTGAA GAAGCTAGGA AAATTGTGGA GGAAGCAGGA 
GGCTTAAAAT CTTTTTTGTT AGATTCTCTT CGTTTTATTA TGATAGACAA TTATATTGGA 
TTGATGAAAG ATGCTGTTCA ACTTAAGAAA AATAAGACCA CTGGAACTGA TGGGGAAGAA 
AGTACTAGTT TGGATGAGAA AGACTCAATG ATTTTCTCTC CACGCTTTCA GAAAAATGCT 
TCCCAGCTCA AACTACAGTT GAATCCAGCA GCTAAAGAAT TTAAACCAGT AACTTGTAAT 
TCTTTCATAC CAGCACCTAA AAATAAAAAA ACAAATAAGA CTGCAAAGTA TGTAACAACA 
GGATATGTGT CATATATTCC TTTTAATACA GTTTTTTCTA ATCAAGGATT TGACACCAGT 
AACACATCAG TGTCTTATGC AAATATGTTG CCACAGTCAT CTCAATGCAT TAGTTTATAT 
ACAACTGTAT CTGATATATC TTCTGATTAT CCAAAAAAAA GAAAACTACC ATTGGTGTCA 
GCTGTACCTC CTATGACAGA TAATGACAAA AGAGATAATT TTGACTCTGG CAATTCCGAG 
GCATGTAATG TAAAATCTGA AAGACCAATG CTTGAAAAAG TATGTTCCTC TGGTAAACCT 
AATTGTGAAA AAAAGATCCG TCAGAATACT CAGAGAACAA CTCTGAAGGC AGAAATATGT 
AAAAATAATA CTGCCAAAGA TGCATGCCTT GGAAGTAATG ATCATTGTGA TCTTGGCACT 
AGTAAACTAG AACGAGAGAT GAAAAATACG ACTATCATCA AAAATACTCC ATGTATACGA 
ATGGTTGCCA TACAGGTAAA AACTGACTTA ATGCATCAGG AGGTTAATAC ACTGCCTTTT 
GGTCCATTTG AAACCCAACA AGGTGATATT TTACGAATGG AAAAGGAACA TCAGGTGTTA 
CAAGAGCAAC TTAAAGAAGC AAGGGAAAAA TATGAACAGT TAAAATGCAG AAGTACAGAG 
GAAACCAGAG AGGTGGAAGA AAAACTGAAA AGAACAATAG AAGAAAATAA GATATCCAAG 
ACTGAATTGA ATTGGTTCCA TCAAGATTTG GAAATAGAAG TGAAAAAATG GCAACAAGAA 
AAAAAGGAAA ATCAAGAAAA ATTGAAAACT TTAAGAAATA AAGTTAAAAA GCTTAATGAT 
ACTCATGAAA TATATTCAAG AAATAATGAA GAAAAGGATC AACAGTATGC ATCACACTTG 
GATGAATTTC TCAAAATCAG TAATAAATTT GCAAATGAAA AATTGAAAAT GGAGGAACTT 
ATAAAAAAGG GAAAAGAGAA TTATGAGGAA ACCATGAAAA GAGCTGTGGC TGCAGAGGTG 
TCCATACTTG AAAATTGGAA GACTACTGAT GTGAGTAAGT TACAGAACAC TGCATCAAAA 
GCAGAAATGT ATGTGAAGAA TCTAAAGTTA ATGAACAGTG ATTCTGCATC ATCTCTTAAT 
TTGGAATCAG AGATTACTCA CTGGGAATCA TTTATTTCAG AAATAAAAAA AGAAATTGAG 
AAGGCAGAGT TTGAGTTTGA GGATCGAATT CAGAAAGTTA AAGAAGGTTC TCAACTGGAT 
AAACTTTCTA AAGTAAAAGT TCCTGAATTT CCATTACCTA CGGATAATTT ATTAATTCAA 
GACAAGCCTG CTGATATTAA TGATCCAGCC TCTGTGACAT ATTCTGCACA AGTTCATTCT 
GACTTATTTT CTGAATATGA TGATCAAAGT CCAGTGATTA TCCCAGCTGA CTTATTAACT 
GGAAGCCAAG CCCTGACACT TCAGAACACA AATCTACATT CTGGCAGTGA CTGCTCAGAG 
GAGATAACTT CTGTACTTTT ACAAGCATCT TATACTGATC CATGTCAGGT CACCCAGCCA 
TCACCGATTG TTAAGAGTTC TGAAAAGGAT ACTCAAATTA ATCAAAGTTC CAAAGGTGTT 
TCACAGACAC ATGCCAGTAC TCCAAACCAG CCACCTTTTA ACAATGTGAT TGAGCATCTG 
TCAACCATAT TCCCAAATTA CAGCAGTGTT GAGCTTTTGA ATTTTATCCA AAAAGTACAA 
AATCAAGACA GAACTACACA TGCAAGTTTG GAGTCTGATG AAATTGTCAG AAGAGTAACA 
GAACTTATTC TAGATGAGCA GAATAAAAAA CTGGCAGTTC AAGGAACAGA CATGAGTGGT 
GCAGCTGAAT CGTCTGATCT TAGACATTCA GCAGAAAATG TATCAGTATC TAGTCCCTCC 
ATAGTCCCAG CTTCAGAAAC ACTTGCCAGC AAACCCTGGG TAGTTGTTGG AGGACCCTCC 
AAGTCAAAGT GGCAAAAATC ACATGATTCC TCTGTCTCCA GTGATGATCC CTGCATAATA 
TGCTATGAAG AGCTAAACCA GGAACATGTA TGTGAGATGG ATTGTGGGCA CCAGTTTCAT 
AAATGGGCAA CAGCTACTGA CCCTGAAATC CCACTGCTGA TCAACACAGA AGATCTGACC 
TACTCTGTTT CTGACTCGGG CCAGTTTACC CCTCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_031959268.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031815128.1 Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

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CloneID	OSa229306
Clone ID Related Accession (Same CDS sequence)	XM_031959268.1
Accession Version	XM_031959268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTT CCATAACTGA AGCAGATTTA ATTGGCAGCA GCTACGTTCG CATTACTAAA 
CTTTGCTGTG AACAGGGTGG GGCATATCCT GCAAAATATT CACAAGTTAA GGCAAATCTA 
GAACTGGATA TCTGCAAAAT CTGGTGTAGT AAGCCACTTC ATGTCCTTCG AGATTACTGT 
GATGTCATTA AAATATACAT CTTCTGGCCA CTTCTTTTTC AACGAGAACA GACTCCTGTA 
GTGTCTCAAT TACATCCTTG TATAGATGTC AGCAGCTCTA GTACTTGTGA ATTAAGTTTG 
AAGAGACTAC AGCATATTGA ATTGCTGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTA 
GATGATCAAT TTTTTATTGG TGGTATATTG AGAATTGGTT ATAAAATAGA AAATAAAATC 
TTGGCAATTG AGGAGGCTTT CAACTGGGTG AAATATACAG GGGAATTCAG AGTTTTGCGT 
AAATTGGAAA GAGCTGAAAA TTGTTGGCCT ATGCTAAGTA TTTTCTTTAC TGAATACAAG 
TACCATATAA CCAAAGTTGT CCTGGAAGAC TGTAATTTAG TTGAAGAATT TGAATATCAG 
AATTGTGCTG CTTGTATAAA GGAAGGTGAA ATTATGAAAA CAAGAGGAAA TGAAGAATTC 
TCAGAAGAGA GGTTTGATAC AGCTATCACA TATTATACCA GAGCAATTGA ATTTAGACCT 
GAAAACCATC TTCTTTATAG TAACCGAGCT CTTTGTTTCC TTAGGACTGG TCAATTCAAG 
AGTGCACTTG GTGATGGAAA GAGGGCCACT ATTCTGAAAT ACAATTGGCC AAAGGGCCAT 
TATCGTTTTT GCGATGCTCT TTCAATGTTG GGGGAGCATG CCTGGGCACT AGAAGCAAAT 
GAGAGAGCTC AACATCTTTG CCGAAACTAT CCAGATGGAA TGAAGGATCT TACTCAACAG 
AACATAAAAT TACAAAAACA AGTGGAAGAA TTAAGAGGTT CAAAACATGG TAGTCATCAG 
ATAAGGAAAT CGTTTTATGG AAAAAGGTTA TCCTCAGGTT ATAATTCTCC AAGACCTTTA 
CATTATGGTT TTCTTAATTT TATGGAAACT AATGAAGAAA ACAAGGTATT GAGATTAGCA 
TCGAAATATC GTGATTATTA TCATTATCAG AATTTGAAGG TATCTCGTGA AATTATGAAA 
TTAGAAAATA AAGACTATCC TCCAGATTTG CCACCAGGTC ATAATCCTAA ATTTAAAGGA 
AAATCAAAGA ATAAAAGCGA TGATGTAGGA AAACCAAGCC TACAACTGAA TTTACAAATA 
GATTTACAAA GCTTATTGGA CAAACAATTC AGTAAATCTT CCAGAGCTGT ACAACAGGAT 
TTTGCTATAT TGATAAAAAT GCTGCAGTCC CTCATCGAAG AAGGTTGTAC AGCTTTGATG 
GACCAACATT GTCGTAGTGC AGCCAAAGCT TTTTCACGGT TGTTAAGTGA TTTAGATCCC 
AATAAATTGA AGCAACTGAA CCTTGCCCTT ATTAATTATG TCCTAGTAAT CTATGGTTAT 
GCCATCTCTC TCCTTGGAAT AGGAATGCCT GAGGAATTAT CAGAGGCTGA ATATCAATTT 
AAGAGAATTA TTGAGCAATA CCCCAATGAA GGAGTTGATT GTTTAGCCTA TTTTGGAATT 
GGAAAAGTAT ATTTGAAAAA AAATAGATTT TCAGATGCAC TTGGTCATTT TCAAAAATCT 
AAAACTATGA TTAAGCTTAT TCCTGGAGTG TTGACTTGGC CTACAACTAA TGTGATTATT 
GAAGAGTCTC AAGCTGAGAA ATTGCAGTTG ATATTGGAAA ATCATATTGA AGAATGCAAA 
TTTCCACCAG ATCCAGATGC AGTTTGTTGT TATCAGAAGT GCAATGGATA TTCCAAAATC 
CAGATATATT TAAATGATCC AGACTTTAAG GGTTTTATAC GAATTAACTG CTGCCAGTTT 
TGTAGAATAG AATTTCATAT TAGCTGTTGG AAAAAATTAA AAACAACAAT TTTCAATGAT 
AAAAATGACA AGGATTTTCT CCAAGGAACA TGTCTTACTC CTGACTGTAG AGGTATTATT 
TCGAAGATTG TTATCTTTGG TGCAGCTGGC CATATTAAAT GTGAATTTGA ACATAGGGTC 
ACAAAAGAAA GGTATCCACC AAGACCTGTT ATGAAGCAGA AATGTTCTAG CTTAGAGAAA 
TTGAAAATTA AAGAAGAAAA AAAAGTAAAG AGAAAACTTC AAAAAAAAGA AGCACAAAAA 
ATAGCAAAAG AGAGAATGGA AGAGAAAACT AGAGAAAATA ATTCATCTAA GCATGGCGAC 
CATAAAGGAT TTGTACAAAG TTGCCATTTA CTTGATGATA GGATTTTGCT GTGTATAAAA 
CAAAATGCCG AGCAAATTAA ATCTGGCATC CAGGACGCTT CCAAACTTCT CAGAGAATTA 
CTTTCTTGGA AAGTAATAAA CACAGAGGAT TATGCAGATA CTTATTTTAA TAGCGGTTTT 
CTAAGTGAAA AAATGGAGCA TCTCATTGAC CATTTGGTAC GGAAAAACAA TAGAGTAAAT 
ACAAGGATTT TTCTGCATGT ACTGAGTGAG ATTGAAGAAA TAAATCCCAA ACTACGTGAC 
TGGATCCAGA AACTCAATAA TTTAGGTTTG GATGCTACAA GGACGTTTTT TTCTCGTTAT 
GGTGATTCTG TTAAAAAGCT TGATTTTAAA TTCATATCTA TGCTCTGGGT TGAAAAATAT 
GGTCATAAAC TAGATCACAT AATATCCAGT TATGAAGAGA GAAAGATAGT TGAATATTCC 
AATAATCAAG GATCATTGAA GGAAGCTCGT TATTTTATAT GGCTATTAGA AGAAAATAGA 
GAAAAGTTTC CAGCATTGCA TAATGCTTTA GATGAATTCT TTGATATAAT GGATGGTCCT 
TGCACAATAT TAAAGAAGCA AGAGACTGAA GATATTTCAT CTAATGGTAT TAAGGTAAAA 
AACAAAACCA GAAAAAAGAA GCACAAGGAG TCAAAGCCTG TTATCATACT CTCTGGAGTT 
GGTGGAGTAT CACAAAATGA TGAGAAAGTT GGTACAGATG AAAGTACTTT ACTTCAATCA 
AATTCTTATG ACCCATTTGT AGTACCCGAA TATCTTCGAG ATGAAGTAGA AGAATTTGAA 
GCTCTATATG AAAAAAATAG TTCAAATAAT TGTAATTATC AGAAGTTATT GGACAGTAAC 
TTAGATCCAA AATGTGAACC ATTATATGAC TATTTTTCTC AGATCCTGGA AGAACATGGT 
CCTTTGGAAA TTGATGACAA ATTATTGGTT GGAGAATATG AACATTTTCC TGAAGAAGCT 
AGGAAAATTG TGGAGGAAGC AGGAGGCTTA AAATCTTTTT TGTTAGATTC TCTTCGTTTT 
ATTATGATAG ACAATTATAT TGGATTGATG AAAGATGCTG TTCAACTTAA GAAAAATAAG 
ACCACTGGAA CTGATGGGGA AGAAAGTACT AGTTTGGATG AGAAAGACTC AATGATTTTC 
TCTCCACGCT TTCAGAAAAA TGCTTCCCAG CTCAAACTAC AGTTGAATCC AGCAGCTAAA 
GAATTTAAAC CAGTAACTTG TAATTCTTTC ATACCAGCAC CTAAAAATAA AAAAACAAAT 
AAGACTGCAA AGTATGTAAC AACAGGATAT GTGTCATATA TTCCTTTTAA TACAGTTTTT 
TCTAATCAAG GATTTGACAC CAGTAACACA TCAGTGTCTT ATGCAAATAT GTTGCCACAG 
TCATCTCAAT GCATTAGTTT ATATACAACT GTATCTGATA TATCTTCTGA TTATCCAAAA 
AAAAGAAAAC TACCATTGGT GTCAGCTGTA CCTCCTATGA CAGATAATGA CAAAAGAGAT 
AATTTTGACT CTGGCAATTC CGAGGCATGT AATGTAAAAT CTGAAAGACC AATGCTTGAA 
AAAGTATGTT CCTCTGGTAA ACCTAATTGT GAAAAAAAGA TCCGTCAGAA TACTCAGAGA 
ACAACTCTGA AGGCAGAAAT ATGTAAAAAT AATACTGCCA AAGATGCATG CCTTGGAAGT 
AATGATCATT GTGATCTTGG CACTAGTAAA CTAGAACGAG AGATGAAAAA TACGACTATC 
ATCAAAAATA CTCCATGTAT ACGAATGGTT GCCATACAGG TAAAAACTGA CTTAATGCAT 
CAGGAGGTTA ATACACTGCC TTTTGGTCCA TTTGAAACCC AACAAGGTGA TATTTTACGA 
ATGGAAAAGG AACATCAGGT GTTACAAGAG CAACTTAAAG AAGCAAGGGA AAAATATGAA 
CAGTTAAAAT GCAGAAGTAC AGAGGAAACC AGAGAGGTGG AAGAAAAACT GAAAAGAACA 
ATAGAAGAAA ATAAGATATC CAAGACTGAA TTGAATTGGT TCCATCAAGA TTTGGAAATA 
GAAGTGAAAA AATGGCAACA AGAAAAAAAG GAAAATCAAG AAAAATTGAA AACTTTAAGA 
AATAAAGTTA AAAAGCTTAA TGATACTCAT GAAATATATT CAAGAAATAA TGAAGAAAAG 
GATCAACAGT ATGCATCACA CTTGGATGAA TTTCTCAAAA TCAGTAATAA ATTTGCAAAT 
GAAAAATTGA AAATGGAGGA ACTTATAAAA AAGGGAAAAG AGAATTATGA GGAAACCATG 
AAAAGAGCTG TGGCTGCAGA GGTGTCCATA CTTGAAAATT GGAAGACTAC TGATGTGAGT 
AAGTTACAGA ACACTGCATC AAAAGCAGAA ATGTATGTGA AGAATCTAAA GTTAATGAAC 
AGTGATTCTG CATCATCTCT TAATTTGGAA TCAGAGATTA CTCACTGGGA ATCATTTATT 
TCAGAAATAA AAAAAGAAAT TGAGAAGGCA GAGTTTGAGT TTGAGGATCG AATTCAGAAA 
GTTAAAGAAG GTTCTCAACT GGATAAACTT TCTAAAGTAA AAGTTCCTGA ATTTCCATTA 
CCTACGGATA ATTTATTAAT TCAAGACAAG CCTGCTGATA TTAATGATCC AGCCTCTGTG 
ACATATTCTG CACAAGTTCA TTCTGACTTA TTTTCTGAAT ATGATGATCA AAGTCCAGTG 
ATTATCCCAG CTGACTTATT AACTGGAAGC CAAGCCCTGA CACTTCAGAA CACAAATCTA 
CATTCTGGCA GTGACTGCTC AGAGGAGATA ACTTCTGTAC TTTTACAAGC ATCTTATACT 
GATCCATGTC AGGTCACCCA GCCATCACCG ATTGTTAAGA GTTCTGAAAA GGATACTCAA 
ATTAATCAAA GTTCCAAAGG TGTTTCACAG ACACATGCCA GTACTCCAAA CCAGCCACCT 
TTTAACAATG TGATTGAGCA TCTGTCAACC ATATTCCCAA ATTACAGCAG TGTTGAGCTT 
TTGAATTTTA TCCAAAAAGT ACAAAATCAA GACAGAACTA CACATGCAAG TTTGGAGTCT 
GATGAAATTG TCAGAAGAGT AACAGAACTT ATTCTAGATG AGCAGAATAA AAAACTGGCA 
GTTCAAGGAA CAGACATGAG TGGTGCAGCT GAATCGTCTG ATCTTAGACA TTCAGCAGAA 
AATGTATCAG TATCTAGTCC CTCCATAGTC CCAGCTTCAG AAACACTTGC CAGCAAACCC 
TGGGTAGTTG TTGGAGGACC CTCCAAGTCA AAGTGGCAAA AATCACATGA TTCCTCTGTC 
TCCAGTGATG ATCCCTGCAT AATATGCTAT GAAGAGCTAA ACCAGGAACA TGTATGTGAG 
ATGGATTGTG GGCACCAGTT TCATAAATGG TGCATTGAGC AGTGGCTTAA AGAACAAAGT 
ACATGCCCAA CATGCCGTGA ATATGTCCTG TTAGCAGAAG AATTTCCTGC ACTTTCTGGA 
TCAGGCCGGC CTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031815128.1
CDS	175..6072
Translation	METSITEADLIGSSYVRITKLCCEQGGAYPAKYSQVKANLELDICKIWCSKPLHVLRDYCDVIKIYIFWPLLFQREQTPVVSQLHPCIDVSSSSTCELSLKRLQHIELLEDIVDLAKKVVDDQFFIGGILRIGYKIENKILAIEEAFNWVKYTGEFRVLRKLERAENCWPMLSIFFTEYKYHITKVVLEDCNLVEEFEYQNCAACIKEGEIMKTRGNEEFSEERFDTAITYYTRAIEFRPENHLLYSNRALCFLRTGQFKSALGDGKRATILKYNWPKGHYRFCDALSMLGEHAWALEANERAQHLCRNYPDGMKDLTQQNIKLQKQVEELRGSKHGSHQIRKSFYGKRLSSGYNSPRPLHYGFLNFMETNEENKVLRLASKYRDYYHYQNLKVSREIMKLENKDYPPDLPPGHNPKFKGKSKNKSDDVGKPSLQLNLQIDLQSLLDKQFSKSSRAVQQDFAILIKMLQSLIEEGCTALMDQHCRSAAKAFSRLLSDLDPNKLKQLNLALINYVLVIYGYAISLLGIGMPEELSEAEYQFKRIIEQYPNEGVDCLAYFGIGKVYLKKNRFSDALGHFQKSKTMIKLIPGVLTWPTTNVIIEESQAEKLQLILENHIEECKFPPDPDAVCCYQKCNGYSKIQIYLNDPDFKGFIRINCCQFCRIEFHISCWKKLKTTIFNDKNDKDFLQGTCLTPDCRGIISKIVIFGAAGHIKCEFEHRVTKERYPPRPVMKQKCSSLEKLKIKEEKKVKRKLQKKEAQKIAKERMEEKTRENNSSKHGDHKGFVQSCHLLDDRILLCIKQNAEQIKSGIQDASKLLRELLSWKVINTEDYADTYFNSGFLSEKMEHLIDHLVRKNNRVNTRIFLHVLSEIEEINPKLRDWIQKLNNLGLDATRTFFSRYGDSVKKLDFKFISMLWVEKYGHKLDHIISSYEERKIVEYSNNQGSLKEARYFIWLLEENREKFPALHNALDEFFDIMDGPCTILKKQETEDISSNGIKVKNKTRKKKHKESKPVIILSGVGGVSQNDEKVGTDESTLLQSNSYDPFVVPEYLRDEVEEFEALYEKNSSNNCNYQKLLDSNLDPKCEPLYDYFSQILEEHGPLEIDDKLLVGEYEHFPEEARKIVEEAGGLKSFLLDSLRFIMIDNYIGLMKDAVQLKKNKTTGTDGEESTSLDEKDSMIFSPRFQKNASQLKLQLNPAAKEFKPVTCNSFIPAPKNKKTNKTAKYVTTGYVSYIPFNTVFSNQGFDTSNTSVSYANMLPQSSQCISLYTTVSDISSDYPKKRKLPLVSAVPPMTDNDKRDNFDSGNSEACNVKSERPMLEKVCSSGKPNCEKKIRQNTQRTTLKAEICKNNTAKDACLGSNDHCDLGTSKLEREMKNTTIIKNTPCIRMVAIQVKTDLMHQEVNTLPFGPFETQQGDILRMEKEHQVLQEQLKEAREKYEQLKCRSTEETREVEEKLKRTIEENKISKTELNWFHQDLEIEVKKWQQEKKENQEKLKTLRNKVKKLNDTHEIYSRNNEEKDQQYASHLDEFLKISNKFANEKLKMEELIKKGKENYEETMKRAVAAEVSILENWKTTDVSKLQNTASKAEMYVKNLKLMNSDSASSLNLESEITHWESFISEIKKEIEKAEFEFEDRIQKVKEGSQLDKLSKVKVPEFPLPTDNLLIQDKPADINDPASVTYSAQVHSDLFSEYDDQSPVIIPADLLTGSQALTLQNTNLHSGSDCSEEITSVLLQASYTDPCQVTQPSPIVKSSEKDTQINQSSKGVSQTHASTPNQPPFNNVIEHLSTIFPNYSSVELLNFIQKVQNQDRTTHASLESDEIVRRVTELILDEQNKKLAVQGTDMSGAAESSDLRHSAENVSVSSPSIVPASETLASKPWVVVGGPSKSKWQKSHDSSVSSDDPCIICYEELNQEHVCEMDCGHQFHKWCIEQWLKEQSTCPTCREYVLLAEEFPALSGSGRPA

Target ORF information:

RefSeq Version	XM_031959268.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031959268.1

ORF Insert Sequence:

ATGGAGACTT CCATAACTGA AGCAGATTTA ATTGGCAGCA GCTACGTTCG CATTACTAAA 
CTTTGCTGTG AACAGGGTGG GGCATATCCT GCAAAATATT CACAAGTTAA GGCAAATCTA 
GAACTGGATA TCTGCAAAAT CTGGTGTAGT AAGCCACTTC ATGTCCTTCG AGATTACTGT 
GATGTCATTA AAATATACAT CTTCTGGCCA CTTCTTTTTC AACGAGAACA GACTCCTGTA 
GTGTCTCAAT TACATCCTTG TATAGATGTC AGCAGCTCTA GTACTTGTGA ATTAAGTTTG 
AAGAGACTAC AGCATATTGA ATTGCTGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTA 
GATGATCAAT TTTTTATTGG TGGTATATTG AGAATTGGTT ATAAAATAGA AAATAAAATC 
TTGGCAATTG AGGAGGCTTT CAACTGGGTG AAATATACAG GGGAATTCAG AGTTTTGCGT 
AAATTGGAAA GAGCTGAAAA TTGTTGGCCT ATGCTAAGTA TTTTCTTTAC TGAATACAAG 
TACCATATAA CCAAAGTTGT CCTGGAAGAC TGTAATTTAG TTGAAGAATT TGAATATCAG 
AATTGTGCTG CTTGTATAAA GGAAGGTGAA ATTATGAAAA CAAGAGGAAA TGAAGAATTC 
TCAGAAGAGA GGTTTGATAC AGCTATCACA TATTATACCA GAGCAATTGA ATTTAGACCT 
GAAAACCATC TTCTTTATAG TAACCGAGCT CTTTGTTTCC TTAGGACTGG TCAATTCAAG 
AGTGCACTTG GTGATGGAAA GAGGGCCACT ATTCTGAAAT ACAATTGGCC AAAGGGCCAT 
TATCGTTTTT GCGATGCTCT TTCAATGTTG GGGGAGCATG CCTGGGCACT AGAAGCAAAT 
GAGAGAGCTC AACATCTTTG CCGAAACTAT CCAGATGGAA TGAAGGATCT TACTCAACAG 
AACATAAAAT TACAAAAACA AGTGGAAGAA TTAAGAGGTT CAAAACATGG TAGTCATCAG 
ATAAGGAAAT CGTTTTATGG AAAAAGGTTA TCCTCAGGTT ATAATTCTCC AAGACCTTTA 
CATTATGGTT TTCTTAATTT TATGGAAACT AATGAAGAAA ACAAGGTATT GAGATTAGCA 
TCGAAATATC GTGATTATTA TCATTATCAG AATTTGAAGG TATCTCGTGA AATTATGAAA 
TTAGAAAATA AAGACTATCC TCCAGATTTG CCACCAGGTC ATAATCCTAA ATTTAAAGGA 
AAATCAAAGA ATAAAAGCGA TGATGTAGGA AAACCAAGCC TACAACTGAA TTTACAAATA 
GATTTACAAA GCTTATTGGA CAAACAATTC AGTAAATCTT CCAGAGCTGT ACAACAGGAT 
TTTGCTATAT TGATAAAAAT GCTGCAGTCC CTCATCGAAG AAGGTTGTAC AGCTTTGATG 
GACCAACATT GTCGTAGTGC AGCCAAAGCT TTTTCACGGT TGTTAAGTGA TTTAGATCCC 
AATAAATTGA AGCAACTGAA CCTTGCCCTT ATTAATTATG TCCTAGTAAT CTATGGTTAT 
GCCATCTCTC TCCTTGGAAT AGGAATGCCT GAGGAATTAT CAGAGGCTGA ATATCAATTT 
AAGAGAATTA TTGAGCAATA CCCCAATGAA GGAGTTGATT GTTTAGCCTA TTTTGGAATT 
GGAAAAGTAT ATTTGAAAAA AAATAGATTT TCAGATGCAC TTGGTCATTT TCAAAAATCT 
AAAACTATGA TTAAGCTTAT TCCTGGAGTG TTGACTTGGC CTACAACTAA TGTGATTATT 
GAAGAGTCTC AAGCTGAGAA ATTGCAGTTG ATATTGGAAA ATCATATTGA AGAATGCAAA 
TTTCCACCAG ATCCAGATGC AGTTTGTTGT TATCAGAAGT GCAATGGATA TTCCAAAATC 
CAGATATATT TAAATGATCC AGACTTTAAG GGTTTTATAC GAATTAACTG CTGCCAGTTT 
TGTAGAATAG AATTTCATAT TAGCTGTTGG AAAAAATTAA AAACAACAAT TTTCAATGAT 
AAAAATGACA AGGATTTTCT CCAAGGAACA TGTCTTACTC CTGACTGTAG AGGTATTATT 
TCGAAGATTG TTATCTTTGG TGCAGCTGGC CATATTAAAT GTGAATTTGA ACATAGGGTC 
ACAAAAGAAA GGTATCCACC AAGACCTGTT ATGAAGCAGA AATGTTCTAG CTTAGAGAAA 
TTGAAAATTA AAGAAGAAAA AAAAGTAAAG AGAAAACTTC AAAAAAAAGA AGCACAAAAA 
ATAGCAAAAG AGAGAATGGA AGAGAAAACT AGAGAAAATA ATTCATCTAA GCATGGCGAC 
CATAAAGGAT TTGTACAAAG TTGCCATTTA CTTGATGATA GGATTTTGCT GTGTATAAAA 
CAAAATGCCG AGCAAATTAA ATCTGGCATC CAGGACGCTT CCAAACTTCT CAGAGAATTA 
CTTTCTTGGA AAGTAATAAA CACAGAGGAT TATGCAGATA CTTATTTTAA TAGCGGTTTT 
CTAAGTGAAA AAATGGAGCA TCTCATTGAC CATTTGGTAC GGAAAAACAA TAGAGTAAAT 
ACAAGGATTT TTCTGCATGT ACTGAGTGAG ATTGAAGAAA TAAATCCCAA ACTACGTGAC 
TGGATCCAGA AACTCAATAA TTTAGGTTTG GATGCTACAA GGACGTTTTT TTCTCGTTAT 
GGTGATTCTG TTAAAAAGCT TGATTTTAAA TTCATATCTA TGCTCTGGGT TGAAAAATAT 
GGTCATAAAC TAGATCACAT AATATCCAGT TATGAAGAGA GAAAGATAGT TGAATATTCC 
AATAATCAAG GATCATTGAA GGAAGCTCGT TATTTTATAT GGCTATTAGA AGAAAATAGA 
GAAAAGTTTC CAGCATTGCA TAATGCTTTA GATGAATTCT TTGATATAAT GGATGGTCCT 
TGCACAATAT TAAAGAAGCA AGAGACTGAA GATATTTCAT CTAATGGTAT TAAGGTAAAA 
AACAAAACCA GAAAAAAGAA GCACAAGGAG TCAAAGCCTG TTATCATACT CTCTGGAGTT 
GGTGGAGTAT CACAAAATGA TGAGAAAGTT GGTACAGATG AAAGTACTTT ACTTCAATCA 
AATTCTTATG ACCCATTTGT AGTACCCGAA TATCTTCGAG ATGAAGTAGA AGAATTTGAA 
GCTCTATATG AAAAAAATAG TTCAAATAAT TGTAATTATC AGAAGTTATT GGACAGTAAC 
TTAGATCCAA AATGTGAACC ATTATATGAC TATTTTTCTC AGATCCTGGA AGAACATGGT 
CCTTTGGAAA TTGATGACAA ATTATTGGTT GGAGAATATG AACATTTTCC TGAAGAAGCT 
AGGAAAATTG TGGAGGAAGC AGGAGGCTTA AAATCTTTTT TGTTAGATTC TCTTCGTTTT 
ATTATGATAG ACAATTATAT TGGATTGATG AAAGATGCTG TTCAACTTAA GAAAAATAAG 
ACCACTGGAA CTGATGGGGA AGAAAGTACT AGTTTGGATG AGAAAGACTC AATGATTTTC 
TCTCCACGCT TTCAGAAAAA TGCTTCCCAG CTCAAACTAC AGTTGAATCC AGCAGCTAAA 
GAATTTAAAC CAGTAACTTG TAATTCTTTC ATACCAGCAC CTAAAAATAA AAAAACAAAT 
AAGACTGCAA AGTATGTAAC AACAGGATAT GTGTCATATA TTCCTTTTAA TACAGTTTTT 
TCTAATCAAG GATTTGACAC CAGTAACACA TCAGTGTCTT ATGCAAATAT GTTGCCACAG 
TCATCTCAAT GCATTAGTTT ATATACAACT GTATCTGATA TATCTTCTGA TTATCCAAAA 
AAAAGAAAAC TACCATTGGT GTCAGCTGTA CCTCCTATGA CAGATAATGA CAAAAGAGAT 
AATTTTGACT CTGGCAATTC CGAGGCATGT AATGTAAAAT CTGAAAGACC AATGCTTGAA 
AAAGTATGTT CCTCTGGTAA ACCTAATTGT GAAAAAAAGA TCCGTCAGAA TACTCAGAGA 
ACAACTCTGA AGGCAGAAAT ATGTAAAAAT AATACTGCCA AAGATGCATG CCTTGGAAGT 
AATGATCATT GTGATCTTGG CACTAGTAAA CTAGAACGAG AGATGAAAAA TACGACTATC 
ATCAAAAATA CTCCATGTAT ACGAATGGTT GCCATACAGG TAAAAACTGA CTTAATGCAT 
CAGGAGGTTA ATACACTGCC TTTTGGTCCA TTTGAAACCC AACAAGGTGA TATTTTACGA 
ATGGAAAAGG AACATCAGGT GTTACAAGAG CAACTTAAAG AAGCAAGGGA AAAATATGAA 
CAGTTAAAAT GCAGAAGTAC AGAGGAAACC AGAGAGGTGG AAGAAAAACT GAAAAGAACA 
ATAGAAGAAA ATAAGATATC CAAGACTGAA TTGAATTGGT TCCATCAAGA TTTGGAAATA 
GAAGTGAAAA AATGGCAACA AGAAAAAAAG GAAAATCAAG AAAAATTGAA AACTTTAAGA 
AATAAAGTTA AAAAGCTTAA TGATACTCAT GAAATATATT CAAGAAATAA TGAAGAAAAG 
GATCAACAGT ATGCATCACA CTTGGATGAA TTTCTCAAAA TCAGTAATAA ATTTGCAAAT 
GAAAAATTGA AAATGGAGGA ACTTATAAAA AAGGGAAAAG AGAATTATGA GGAAACCATG 
AAAAGAGCTG TGGCTGCAGA GGTGTCCATA CTTGAAAATT GGAAGACTAC TGATGTGAGT 
AAGTTACAGA ACACTGCATC AAAAGCAGAA ATGTATGTGA AGAATCTAAA GTTAATGAAC 
AGTGATTCTG CATCATCTCT TAATTTGGAA TCAGAGATTA CTCACTGGGA ATCATTTATT 
TCAGAAATAA AAAAAGAAAT TGAGAAGGCA GAGTTTGAGT TTGAGGATCG AATTCAGAAA 
GTTAAAGAAG GTTCTCAACT GGATAAACTT TCTAAAGTAA AAGTTCCTGA ATTTCCATTA 
CCTACGGATA ATTTATTAAT TCAAGACAAG CCTGCTGATA TTAATGATCC AGCCTCTGTG 
ACATATTCTG CACAAGTTCA TTCTGACTTA TTTTCTGAAT ATGATGATCA AAGTCCAGTG 
ATTATCCCAG CTGACTTATT AACTGGAAGC CAAGCCCTGA CACTTCAGAA CACAAATCTA 
CATTCTGGCA GTGACTGCTC AGAGGAGATA ACTTCTGTAC TTTTACAAGC ATCTTATACT 
GATCCATGTC AGGTCACCCA GCCATCACCG ATTGTTAAGA GTTCTGAAAA GGATACTCAA 
ATTAATCAAA GTTCCAAAGG TGTTTCACAG ACACATGCCA GTACTCCAAA CCAGCCACCT 
TTTAACAATG TGATTGAGCA TCTGTCAACC ATATTCCCAA ATTACAGCAG TGTTGAGCTT 
TTGAATTTTA TCCAAAAAGT ACAAAATCAA GACAGAACTA CACATGCAAG TTTGGAGTCT 
GATGAAATTG TCAGAAGAGT AACAGAACTT ATTCTAGATG AGCAGAATAA AAAACTGGCA 
GTTCAAGGAA CAGACATGAG TGGTGCAGCT GAATCGTCTG ATCTTAGACA TTCAGCAGAA 
AATGTATCAG TATCTAGTCC CTCCATAGTC CCAGCTTCAG AAACACTTGC CAGCAAACCC 
TGGGTAGTTG TTGGAGGACC CTCCAAGTCA AAGTGGCAAA AATCACATGA TTCCTCTGTC 
TCCAGTGATG ATCCCTGCAT AATATGCTAT GAAGAGCTAA ACCAGGAACA TGTATGTGAG 
ATGGATTGTG GGCACCAGTT TCATAAATGG TGCATTGAGC AGTGGCTTAA AGAACAAAGT 
ACATGCCCAA CATGCCGTGA ATATGTCCTG TTAGCAGAAG AATTTCCTGC ACTTTCTGGA 
TCAGGCCGGC CTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_031959269.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031815129.1 Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

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CloneID	OSa229307
Clone ID Related Accession (Same CDS sequence)	XM_031959269.1
Accession Version	XM_031959269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase TTC3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTT CCATAACTGA AGCAGATTTA ATTGGCAGCA GCTACGTTCG CATTACTAAA 
CTTTGCTGTG AACAGGGTGG GGCATATCCT GCAAAATATT CACAAGTTAA GGCAAATCTA 
GAACTGGATA TCTGCAAAAT CTGGTGTAGT AAGCCACTTC ATGTCCTTCG AGATTACTGT 
GATGTCATTA AAATATACAT CTTCTGGCCA CTTCTTTTTC AACGAGAACA GACTCCTGTA 
GTGTCTCAAT TACATCCTTG TATAGATGTC AGCTCTAGTA CTTGTGAATT AAGTTTGAAG 
AGACTACAGC ATATTGAATT GCTGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTAGAT 
GATCAATTTT TTATTGGTGG TATATTGAGA ATTGGTTATA AAATAGAAAA TAAAATCTTG 
GCAATTGAGG AGGCTTTCAA CTGGGTGAAA TATACAGGGG AATTCAGAGT TTTGCGTAAA 
TTGGAAAGAG CTGAAAATTG TTGGCCTATG CTAAGTATTT TCTTTACTGA ATACAAGTAC 
CATATAACCA AAGTTGTCCT GGAAGACTGT AATTTAGTTG AAGAATTTGA ATATCAGAAT 
TGTGCTGCTT GTATAAAGGA AGGTGAAATT ATGAAAACAA GAGGAAATGA AGAATTCTCA 
GAAGAGAGGT TTGATACAGC TATCACATAT TATACCAGAG CAATTGAATT TAGACCTGAA 
AACCATCTTC TTTATAGTAA CCGAGCTCTT TGTTTCCTTA GGACTGGTCA ATTCAAGAGT 
GCACTTGGTG ATGGAAAGAG GGCCACTATT CTGAAATACA ATTGGCCAAA GGGCCATTAT 
CGTTTTTGCG ATGCTCTTTC AATGTTGGGG GAGCATGCCT GGGCACTAGA AGCAAATGAG 
AGAGCTCAAC ATCTTTGCCG AAACTATCCA GATGGAATGA AGGATCTTAC TCAACAGAAC 
ATAAAATTAC AAAAACAAGT GGAAGAATTA AGAGGTTCAA AACATGGTAG TCATCAGATA 
AGGAAATCGT TTTATGGAAA AAGGTTATCC TCAGGTTATA ATTCTCCAAG ACCTTTACAT 
TATGGTTTTC TTAATTTTAT GGAAACTAAT GAAGAAAACA AGGTATTGAG ATTAGCATCG 
AAATATCGTG ATTATTATCA TTATCAGAAT TTGAAGGTAT CTCGTGAAAT TATGAAATTA 
GAAAATAAAG ACTATCCTCC AGATTTGCCA CCAGGTCATA ATCCTAAATT TAAAGGAAAA 
TCAAAGAATA AAAGCGATGA TGTAGGAAAA CCAAGCCTAC AACTGAATTT ACAAATAGAT 
TTACAAAGCT TATTGGACAA ACAATTCAGT AAATCTTCCA GAGCTGTACA ACAGGATTTT 
GCTATATTGA TAAAAATGCT GCAGTCCCTC ATCGAAGAAG GTTGTACAGC TTTGATGGAC 
CAACATTGTC GTAGTGCAGC CAAAGCTTTT TCACGGTTGT TAAGTGATTT AGATCCCAAT 
AAATTGAAGC AACTGAACCT TGCCCTTATT AATTATGTCC TAGTAATCTA TGGTTATGCC 
ATCTCTCTCC TTGGAATAGG AATGCCTGAG GAATTATCAG AGGCTGAATA TCAATTTAAG 
AGAATTATTG AGCAATACCC CAATGAAGGA GTTGATTGTT TAGCCTATTT TGGAATTGGA 
AAAGTATATT TGAAAAAAAA TAGATTTTCA GATGCACTTG GTCATTTTCA AAAATCTAAA 
ACTATGATTA AGCTTATTCC TGGAGTGTTG ACTTGGCCTA CAACTAATGT GATTATTGAA 
GAGTCTCAAG CTGAGAAATT GCAGTTGATA TTGGAAAATC ATATTGAAGA ATGCAAATTT 
CCACCAGATC CAGATGCAGT TTGTTGTTAT CAGAAGTGCA ATGGATATTC CAAAATCCAG 
ATATATTTAA ATGATCCAGA CTTTAAGGGT TTTATACGAA TTAACTGCTG CCAGTTTTGT 
AGAATAGAAT TTCATATTAG CTGTTGGAAA AAATTAAAAA CAACAATTTT CAATGATAAA 
AATGACAAGG ATTTTCTCCA AGGAACATGT CTTACTCCTG ACTGTAGAGG TATTATTTCG 
AAGATTGTTA TCTTTGGTGC AGCTGGCCAT ATTAAATGTG AATTTGAACA TAGGGTCACA 
AAAGAAAGGT ATCCACCAAG ACCTGTTATG AAGCAGAAAT GTTCTAGCTT AGAGAAATTG 
AAAATTAAAG AAGAAAAAAA AGTAAAGAGA AAACTTCAAA AAAAAGAAGC ACAAAAAATA 
GCAAAAGAGA GAATGGAAGA GAAAACTAGA GAAAATAATT CATCTAAGCA TGGCGACCAT 
AAAGGATTTG TACAAAGTTG CCATTTACTT GATGATAGGA TTTTGCTGTG TATAAAACAA 
AATGCCGAGC AAATTAAATC TGGCATCCAG GACGCTTCCA AACTTCTCAG AGAATTACTT 
TCTTGGAAAG TAATAAACAC AGAGGATTAT GCAGATACTT ATTTTAATAG CGGTTTTCTA 
AGTGAAAAAA TGGAGCATCT CATTGACCAT TTGGTACGGA AAAACAATAG AGTAAATACA 
AGGATTTTTC TGCATGTACT GAGTGAGATT GAAGAAATAA ATCCCAAACT ACGTGACTGG 
ATCCAGAAAC TCAATAATTT AGGTTTGGAT GCTACAAGGA CGTTTTTTTC TCGTTATGGT 
GATTCTGTTA AAAAGCTTGA TTTTAAATTC ATATCTATGC TCTGGGTTGA AAAATATGGT 
CATAAACTAG ATCACATAAT ATCCAGTTAT GAAGAGAGAA AGATAGTTGA ATATTCCAAT 
AATCAAGGAT CATTGAAGGA AGCTCGTTAT TTTATATGGC TATTAGAAGA AAATAGAGAA 
AAGTTTCCAG CATTGCATAA TGCTTTAGAT GAATTCTTTG ATATAATGGA TGGTCCTTGC 
ACAATATTAA AGAAGCAAGA GACTGAAGAT ATTTCATCTA ATGGTATTAA GGTAAAAAAC 
AAAACCAGAA AAAAGAAGCA CAAGGAGTCA AAGCCTGTTA TCATACTCTC TGGAGTTGGT 
GGAGTATCAC AAAATGATGA GAAAGTTGGT ACAGATGAAA GTACTTTACT TCAATCAAAT 
TCTTATGACC CATTTGTAGT ACCCGAATAT CTTCGAGATG AAGTAGAAGA ATTTGAAGCT 
CTATATGAAA AAAATAGTTC AAATAATTGT AATTATCAGA AGTTATTGGA CAGTAACTTA 
GATCCAAAAT GTGAACCATT ATATGACTAT TTTTCTCAGA TCCTGGAAGA ACATGGTCCT 
TTGGAAATTG ATGACAAATT ATTGGTTGGA GAATATGAAC ATTTTCCTGA AGAAGCTAGG 
AAAATTGTGG AGGAAGCAGG AGGCTTAAAA TCTTTTTTGT TAGATTCTCT TCGTTTTATT 
ATGATAGACA ATTATATTGG ATTGATGAAA GATGCTGTTC AACTTAAGAA AAATAAGACC 
ACTGGAACTG ATGGGGAAGA AAGTACTAGT TTGGATGAGA AAGACTCAAT GATTTTCTCT 
CCACGCTTTC AGAAAAATGC TTCCCAGCTC AAACTACAGT TGAATCCAGC AGCTAAAGAA 
TTTAAACCAG TAACTTGTAA TTCTTTCATA CCAGCACCTA AAAATAAAAA AACAAATAAG 
ACTGCAAAGT ATGTAACAAC AGGATATGTG TCATATATTC CTTTTAATAC AGTTTTTTCT 
AATCAAGGAT TTGACACCAG TAACACATCA GTGTCTTATG CAAATATGTT GCCACAGTCA 
TCTCAATGCA TTAGTTTATA TACAACTGTA TCTGATATAT CTTCTGATTA TCCAAAAAAA 
AGAAAACTAC CATTGGTGTC AGCTGTACCT CCTATGACAG ATAATGACAA AAGAGATAAT 
TTTGACTCTG GCAATTCCGA GGCATGTAAT GTAAAATCTG AAAGACCAAT GCTTGAAAAA 
GTATGTTCCT CTGGTAAACC TAATTGTGAA AAAAAGATCC GTCAGAATAC TCAGAGAACA 
ACTCTGAAGG CAGAAATATG TAAAAATAAT ACTGCCAAAG ATGCATGCCT TGGAAGTAAT 
GATCATTGTG ATCTTGGCAC TAGTAAACTA GAACGAGAGA TGAAAAATAC GACTATCATC 
AAAAATACTC CATGTATACG AATGGTTGCC ATACAGGTAA AAACTGACTT AATGCATCAG 
GAGGTTAATA CACTGCCTTT TGGTCCATTT GAAACCCAAC AAGGTGATAT TTTACGAATG 
GAAAAGGAAC ATCAGGTGTT ACAAGAGCAA CTTAAAGAAG CAAGGGAAAA ATATGAACAG 
TTAAAATGCA GAAGTACAGA GGAAACCAGA GAGGTGGAAG AAAAACTGAA AAGAACAATA 
GAAGAAAATA AGATATCCAA GACTGAATTG AATTGGTTCC ATCAAGATTT GGAAATAGAA 
GTGAAAAAAT GGCAACAAGA AAAAAAGGAA AATCAAGAAA AATTGAAAAC TTTAAGAAAT 
AAAGTTAAAA AGCTTAATGA TACTCATGAA ATATATTCAA GAAATAATGA AGAAAAGGAT 
CAACAGTATG CATCACACTT GGATGAATTT CTCAAAATCA GTAATAAATT TGCAAATGAA 
AAATTGAAAA TGGAGGAACT TATAAAAAAG GGAAAAGAGA ATTATGAGGA AACCATGAAA 
AGAGCTGTGG CTGCAGAGGT GTCCATACTT GAAAATTGGA AGACTACTGA TGTGAGTAAG 
TTACAGAACA CTGCATCAAA AGCAGAAATG TATGTGAAGA ATCTAAAGTT AATGAACAGT 
GATTCTGCAT CATCTCTTAA TTTGGAATCA GAGATTACTC ACTGGGAATC ATTTATTTCA 
GAAATAAAAA AAGAAATTGA GAAGGCAGAG TTTGAGTTTG AGGATCGAAT TCAGAAAGTT 
AAAGAAGGTT CTCAACTGGA TAAACTTTCT AAAGTAAAAG TTCCTGAATT TCCATTACCT 
ACGGATAATT TATTAATTCA AGACAAGCCT GCTGATATTA ATGATCCAGC CTCTGTGACA 
TATTCTGCAC AAGTTCATTC TGACTTATTT TCTGAATATG ATGATCAAAG TCCAGTGATT 
ATCCCAGCTG ACTTATTAAC TGGAAGCCAA GCCCTGACAC TTCAGAACAC AAATCTACAT 
TCTGGCAGTG ACTGCTCAGA GGAGATAACT TCTGTACTTT TACAAGCATC TTATACTGAT 
CCATGTCAGG TCACCCAGCC ATCACCGATT GTTAAGAGTT CTGAAAAGGA TACTCAAATT 
AATCAAAGTT CCAAAGGTGT TTCACAGACA CATGCCAGTA CTCCAAACCA GCCACCTTTT 
AACAATGTGA TTGAGCATCT GTCAACCATA TTCCCAAATT ACAGCAGTGT TGAGCTTTTG 
AATTTTATCC AAAAAGTACA AAATCAAGAC AGAACTACAC ATGCAAGTTT GGAGTCTGAT 
GAAATTGTCA GAAGAGTAAC AGAACTTATT CTAGATGAGC AGAATAAAAA ACTGGCAGTT 
CAAGGAACAG ACATGAGTGG TGCAGCTGAA TCGTCTGATC TTAGACATTC AGCAGAAAAT 
GTATCAGTAT CTAGTCCCTC CATAGTCCCA GCTTCAGAAA CACTTGCCAG CAAACCCTGG 
GTAGTTGTTG GAGGACCCTC CAAGTCAAAG TGGCAAAAAT CACATGATTC CTCTGTCTCC 
AGTGATGATC CCTGCATAAT ATGCTATGAA GAGCTAAACC AGGAACATGT ATGTGAGATG 
GATTGTGGGC ACCAGTTTCA TAAATGGTGC ATTGAGCAGT GGCTTAAAGA ACAAAGTACA 
TGCCCAACAT GCCGTGAATA TGTCCTGTTA GCAGAAGAAT TTCCTGCACT TTCTGGATCA 
GGCCGGCCTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031815129.1
CDS	175..6069
Translation	METSITEADLIGSSYVRITKLCCEQGGAYPAKYSQVKANLELDICKIWCSKPLHVLRDYCDVIKIYIFWPLLFQREQTPVVSQLHPCIDVSSSTCELSLKRLQHIELLEDIVDLAKKVVDDQFFIGGILRIGYKIENKILAIEEAFNWVKYTGEFRVLRKLERAENCWPMLSIFFTEYKYHITKVVLEDCNLVEEFEYQNCAACIKEGEIMKTRGNEEFSEERFDTAITYYTRAIEFRPENHLLYSNRALCFLRTGQFKSALGDGKRATILKYNWPKGHYRFCDALSMLGEHAWALEANERAQHLCRNYPDGMKDLTQQNIKLQKQVEELRGSKHGSHQIRKSFYGKRLSSGYNSPRPLHYGFLNFMETNEENKVLRLASKYRDYYHYQNLKVSREIMKLENKDYPPDLPPGHNPKFKGKSKNKSDDVGKPSLQLNLQIDLQSLLDKQFSKSSRAVQQDFAILIKMLQSLIEEGCTALMDQHCRSAAKAFSRLLSDLDPNKLKQLNLALINYVLVIYGYAISLLGIGMPEELSEAEYQFKRIIEQYPNEGVDCLAYFGIGKVYLKKNRFSDALGHFQKSKTMIKLIPGVLTWPTTNVIIEESQAEKLQLILENHIEECKFPPDPDAVCCYQKCNGYSKIQIYLNDPDFKGFIRINCCQFCRIEFHISCWKKLKTTIFNDKNDKDFLQGTCLTPDCRGIISKIVIFGAAGHIKCEFEHRVTKERYPPRPVMKQKCSSLEKLKIKEEKKVKRKLQKKEAQKIAKERMEEKTRENNSSKHGDHKGFVQSCHLLDDRILLCIKQNAEQIKSGIQDASKLLRELLSWKVINTEDYADTYFNSGFLSEKMEHLIDHLVRKNNRVNTRIFLHVLSEIEEINPKLRDWIQKLNNLGLDATRTFFSRYGDSVKKLDFKFISMLWVEKYGHKLDHIISSYEERKIVEYSNNQGSLKEARYFIWLLEENREKFPALHNALDEFFDIMDGPCTILKKQETEDISSNGIKVKNKTRKKKHKESKPVIILSGVGGVSQNDEKVGTDESTLLQSNSYDPFVVPEYLRDEVEEFEALYEKNSSNNCNYQKLLDSNLDPKCEPLYDYFSQILEEHGPLEIDDKLLVGEYEHFPEEARKIVEEAGGLKSFLLDSLRFIMIDNYIGLMKDAVQLKKNKTTGTDGEESTSLDEKDSMIFSPRFQKNASQLKLQLNPAAKEFKPVTCNSFIPAPKNKKTNKTAKYVTTGYVSYIPFNTVFSNQGFDTSNTSVSYANMLPQSSQCISLYTTVSDISSDYPKKRKLPLVSAVPPMTDNDKRDNFDSGNSEACNVKSERPMLEKVCSSGKPNCEKKIRQNTQRTTLKAEICKNNTAKDACLGSNDHCDLGTSKLEREMKNTTIIKNTPCIRMVAIQVKTDLMHQEVNTLPFGPFETQQGDILRMEKEHQVLQEQLKEAREKYEQLKCRSTEETREVEEKLKRTIEENKISKTELNWFHQDLEIEVKKWQQEKKENQEKLKTLRNKVKKLNDTHEIYSRNNEEKDQQYASHLDEFLKISNKFANEKLKMEELIKKGKENYEETMKRAVAAEVSILENWKTTDVSKLQNTASKAEMYVKNLKLMNSDSASSLNLESEITHWESFISEIKKEIEKAEFEFEDRIQKVKEGSQLDKLSKVKVPEFPLPTDNLLIQDKPADINDPASVTYSAQVHSDLFSEYDDQSPVIIPADLLTGSQALTLQNTNLHSGSDCSEEITSVLLQASYTDPCQVTQPSPIVKSSEKDTQINQSSKGVSQTHASTPNQPPFNNVIEHLSTIFPNYSSVELLNFIQKVQNQDRTTHASLESDEIVRRVTELILDEQNKKLAVQGTDMSGAAESSDLRHSAENVSVSSPSIVPASETLASKPWVVVGGPSKSKWQKSHDSSVSSDDPCIICYEELNQEHVCEMDCGHQFHKWCIEQWLKEQSTCPTCREYVLLAEEFPALSGSGRPA

Target ORF information:

RefSeq Version	XM_031959269.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031959269.1

ORF Insert Sequence:

ATGGAGACTT CCATAACTGA AGCAGATTTA ATTGGCAGCA GCTACGTTCG CATTACTAAA 
CTTTGCTGTG AACAGGGTGG GGCATATCCT GCAAAATATT CACAAGTTAA GGCAAATCTA 
GAACTGGATA TCTGCAAAAT CTGGTGTAGT AAGCCACTTC ATGTCCTTCG AGATTACTGT 
GATGTCATTA AAATATACAT CTTCTGGCCA CTTCTTTTTC AACGAGAACA GACTCCTGTA 
GTGTCTCAAT TACATCCTTG TATAGATGTC AGCTCTAGTA CTTGTGAATT AAGTTTGAAG 
AGACTACAGC ATATTGAATT GCTGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTAGAT 
GATCAATTTT TTATTGGTGG TATATTGAGA ATTGGTTATA AAATAGAAAA TAAAATCTTG 
GCAATTGAGG AGGCTTTCAA CTGGGTGAAA TATACAGGGG AATTCAGAGT TTTGCGTAAA 
TTGGAAAGAG CTGAAAATTG TTGGCCTATG CTAAGTATTT TCTTTACTGA ATACAAGTAC 
CATATAACCA AAGTTGTCCT GGAAGACTGT AATTTAGTTG AAGAATTTGA ATATCAGAAT 
TGTGCTGCTT GTATAAAGGA AGGTGAAATT ATGAAAACAA GAGGAAATGA AGAATTCTCA 
GAAGAGAGGT TTGATACAGC TATCACATAT TATACCAGAG CAATTGAATT TAGACCTGAA 
AACCATCTTC TTTATAGTAA CCGAGCTCTT TGTTTCCTTA GGACTGGTCA ATTCAAGAGT 
GCACTTGGTG ATGGAAAGAG GGCCACTATT CTGAAATACA ATTGGCCAAA GGGCCATTAT 
CGTTTTTGCG ATGCTCTTTC AATGTTGGGG GAGCATGCCT GGGCACTAGA AGCAAATGAG 
AGAGCTCAAC ATCTTTGCCG AAACTATCCA GATGGAATGA AGGATCTTAC TCAACAGAAC 
ATAAAATTAC AAAAACAAGT GGAAGAATTA AGAGGTTCAA AACATGGTAG TCATCAGATA 
AGGAAATCGT TTTATGGAAA AAGGTTATCC TCAGGTTATA ATTCTCCAAG ACCTTTACAT 
TATGGTTTTC TTAATTTTAT GGAAACTAAT GAAGAAAACA AGGTATTGAG ATTAGCATCG 
AAATATCGTG ATTATTATCA TTATCAGAAT TTGAAGGTAT CTCGTGAAAT TATGAAATTA 
GAAAATAAAG ACTATCCTCC AGATTTGCCA CCAGGTCATA ATCCTAAATT TAAAGGAAAA 
TCAAAGAATA AAAGCGATGA TGTAGGAAAA CCAAGCCTAC AACTGAATTT ACAAATAGAT 
TTACAAAGCT TATTGGACAA ACAATTCAGT AAATCTTCCA GAGCTGTACA ACAGGATTTT 
GCTATATTGA TAAAAATGCT GCAGTCCCTC ATCGAAGAAG GTTGTACAGC TTTGATGGAC 
CAACATTGTC GTAGTGCAGC CAAAGCTTTT TCACGGTTGT TAAGTGATTT AGATCCCAAT 
AAATTGAAGC AACTGAACCT TGCCCTTATT AATTATGTCC TAGTAATCTA TGGTTATGCC 
ATCTCTCTCC TTGGAATAGG AATGCCTGAG GAATTATCAG AGGCTGAATA TCAATTTAAG 
AGAATTATTG AGCAATACCC CAATGAAGGA GTTGATTGTT TAGCCTATTT TGGAATTGGA 
AAAGTATATT TGAAAAAAAA TAGATTTTCA GATGCACTTG GTCATTTTCA AAAATCTAAA 
ACTATGATTA AGCTTATTCC TGGAGTGTTG ACTTGGCCTA CAACTAATGT GATTATTGAA 
GAGTCTCAAG CTGAGAAATT GCAGTTGATA TTGGAAAATC ATATTGAAGA ATGCAAATTT 
CCACCAGATC CAGATGCAGT TTGTTGTTAT CAGAAGTGCA ATGGATATTC CAAAATCCAG 
ATATATTTAA ATGATCCAGA CTTTAAGGGT TTTATACGAA TTAACTGCTG CCAGTTTTGT 
AGAATAGAAT TTCATATTAG CTGTTGGAAA AAATTAAAAA CAACAATTTT CAATGATAAA 
AATGACAAGG ATTTTCTCCA AGGAACATGT CTTACTCCTG ACTGTAGAGG TATTATTTCG 
AAGATTGTTA TCTTTGGTGC AGCTGGCCAT ATTAAATGTG AATTTGAACA TAGGGTCACA 
AAAGAAAGGT ATCCACCAAG ACCTGTTATG AAGCAGAAAT GTTCTAGCTT AGAGAAATTG 
AAAATTAAAG AAGAAAAAAA AGTAAAGAGA AAACTTCAAA AAAAAGAAGC ACAAAAAATA 
GCAAAAGAGA GAATGGAAGA GAAAACTAGA GAAAATAATT CATCTAAGCA TGGCGACCAT 
AAAGGATTTG TACAAAGTTG CCATTTACTT GATGATAGGA TTTTGCTGTG TATAAAACAA 
AATGCCGAGC AAATTAAATC TGGCATCCAG GACGCTTCCA AACTTCTCAG AGAATTACTT 
TCTTGGAAAG TAATAAACAC AGAGGATTAT GCAGATACTT ATTTTAATAG CGGTTTTCTA 
AGTGAAAAAA TGGAGCATCT CATTGACCAT TTGGTACGGA AAAACAATAG AGTAAATACA 
AGGATTTTTC TGCATGTACT GAGTGAGATT GAAGAAATAA ATCCCAAACT ACGTGACTGG 
ATCCAGAAAC TCAATAATTT AGGTTTGGAT GCTACAAGGA CGTTTTTTTC TCGTTATGGT 
GATTCTGTTA AAAAGCTTGA TTTTAAATTC ATATCTATGC TCTGGGTTGA AAAATATGGT 
CATAAACTAG ATCACATAAT ATCCAGTTAT GAAGAGAGAA AGATAGTTGA ATATTCCAAT 
AATCAAGGAT CATTGAAGGA AGCTCGTTAT TTTATATGGC TATTAGAAGA AAATAGAGAA 
AAGTTTCCAG CATTGCATAA TGCTTTAGAT GAATTCTTTG ATATAATGGA TGGTCCTTGC 
ACAATATTAA AGAAGCAAGA GACTGAAGAT ATTTCATCTA ATGGTATTAA GGTAAAAAAC 
AAAACCAGAA AAAAGAAGCA CAAGGAGTCA AAGCCTGTTA TCATACTCTC TGGAGTTGGT 
GGAGTATCAC AAAATGATGA GAAAGTTGGT ACAGATGAAA GTACTTTACT TCAATCAAAT 
TCTTATGACC CATTTGTAGT ACCCGAATAT CTTCGAGATG AAGTAGAAGA ATTTGAAGCT 
CTATATGAAA AAAATAGTTC AAATAATTGT AATTATCAGA AGTTATTGGA CAGTAACTTA 
GATCCAAAAT GTGAACCATT ATATGACTAT TTTTCTCAGA TCCTGGAAGA ACATGGTCCT 
TTGGAAATTG ATGACAAATT ATTGGTTGGA GAATATGAAC ATTTTCCTGA AGAAGCTAGG 
AAAATTGTGG AGGAAGCAGG AGGCTTAAAA TCTTTTTTGT TAGATTCTCT TCGTTTTATT 
ATGATAGACA ATTATATTGG ATTGATGAAA GATGCTGTTC AACTTAAGAA AAATAAGACC 
ACTGGAACTG ATGGGGAAGA AAGTACTAGT TTGGATGAGA AAGACTCAAT GATTTTCTCT 
CCACGCTTTC AGAAAAATGC TTCCCAGCTC AAACTACAGT TGAATCCAGC AGCTAAAGAA 
TTTAAACCAG TAACTTGTAA TTCTTTCATA CCAGCACCTA AAAATAAAAA AACAAATAAG 
ACTGCAAAGT ATGTAACAAC AGGATATGTG TCATATATTC CTTTTAATAC AGTTTTTTCT 
AATCAAGGAT TTGACACCAG TAACACATCA GTGTCTTATG CAAATATGTT GCCACAGTCA 
TCTCAATGCA TTAGTTTATA TACAACTGTA TCTGATATAT CTTCTGATTA TCCAAAAAAA 
AGAAAACTAC CATTGGTGTC AGCTGTACCT CCTATGACAG ATAATGACAA AAGAGATAAT 
TTTGACTCTG GCAATTCCGA GGCATGTAAT GTAAAATCTG AAAGACCAAT GCTTGAAAAA 
GTATGTTCCT CTGGTAAACC TAATTGTGAA AAAAAGATCC GTCAGAATAC TCAGAGAACA 
ACTCTGAAGG CAGAAATATG TAAAAATAAT ACTGCCAAAG ATGCATGCCT TGGAAGTAAT 
GATCATTGTG ATCTTGGCAC TAGTAAACTA GAACGAGAGA TGAAAAATAC GACTATCATC 
AAAAATACTC CATGTATACG AATGGTTGCC ATACAGGTAA AAACTGACTT AATGCATCAG 
GAGGTTAATA CACTGCCTTT TGGTCCATTT GAAACCCAAC AAGGTGATAT TTTACGAATG 
GAAAAGGAAC ATCAGGTGTT ACAAGAGCAA CTTAAAGAAG CAAGGGAAAA ATATGAACAG 
TTAAAATGCA GAAGTACAGA GGAAACCAGA GAGGTGGAAG AAAAACTGAA AAGAACAATA 
GAAGAAAATA AGATATCCAA GACTGAATTG AATTGGTTCC ATCAAGATTT GGAAATAGAA 
GTGAAAAAAT GGCAACAAGA AAAAAAGGAA AATCAAGAAA AATTGAAAAC TTTAAGAAAT 
AAAGTTAAAA AGCTTAATGA TACTCATGAA ATATATTCAA GAAATAATGA AGAAAAGGAT 
CAACAGTATG CATCACACTT GGATGAATTT CTCAAAATCA GTAATAAATT TGCAAATGAA 
AAATTGAAAA TGGAGGAACT TATAAAAAAG GGAAAAGAGA ATTATGAGGA AACCATGAAA 
AGAGCTGTGG CTGCAGAGGT GTCCATACTT GAAAATTGGA AGACTACTGA TGTGAGTAAG 
TTACAGAACA CTGCATCAAA AGCAGAAATG TATGTGAAGA ATCTAAAGTT AATGAACAGT 
GATTCTGCAT CATCTCTTAA TTTGGAATCA GAGATTACTC ACTGGGAATC ATTTATTTCA 
GAAATAAAAA AAGAAATTGA GAAGGCAGAG TTTGAGTTTG AGGATCGAAT TCAGAAAGTT 
AAAGAAGGTT CTCAACTGGA TAAACTTTCT AAAGTAAAAG TTCCTGAATT TCCATTACCT 
ACGGATAATT TATTAATTCA AGACAAGCCT GCTGATATTA ATGATCCAGC CTCTGTGACA 
TATTCTGCAC AAGTTCATTC TGACTTATTT TCTGAATATG ATGATCAAAG TCCAGTGATT 
ATCCCAGCTG ACTTATTAAC TGGAAGCCAA GCCCTGACAC TTCAGAACAC AAATCTACAT 
TCTGGCAGTG ACTGCTCAGA GGAGATAACT TCTGTACTTT TACAAGCATC TTATACTGAT 
CCATGTCAGG TCACCCAGCC ATCACCGATT GTTAAGAGTT CTGAAAAGGA TACTCAAATT 
AATCAAAGTT CCAAAGGTGT TTCACAGACA CATGCCAGTA CTCCAAACCA GCCACCTTTT 
AACAATGTGA TTGAGCATCT GTCAACCATA TTCCCAAATT ACAGCAGTGT TGAGCTTTTG 
AATTTTATCC AAAAAGTACA AAATCAAGAC AGAACTACAC ATGCAAGTTT GGAGTCTGAT 
GAAATTGTCA GAAGAGTAAC AGAACTTATT CTAGATGAGC AGAATAAAAA ACTGGCAGTT 
CAAGGAACAG ACATGAGTGG TGCAGCTGAA TCGTCTGATC TTAGACATTC AGCAGAAAAT 
GTATCAGTAT CTAGTCCCTC CATAGTCCCA GCTTCAGAAA CACTTGCCAG CAAACCCTGG 
GTAGTTGTTG GAGGACCCTC CAAGTCAAAG TGGCAAAAAT CACATGATTC CTCTGTCTCC 
AGTGATGATC CCTGCATAAT ATGCTATGAA GAGCTAAACC AGGAACATGT ATGTGAGATG 
GATTGTGGGC ACCAGTTTCA TAAATGGTGC ATTGAGCAGT GGCTTAAAGA ACAAAGTACA 
TGCCCAACAT GCCGTGAATA TGTCCTGTTA GCAGAAGAAT TTCCTGCACT TTCTGGATCA 
GGCCGGCCTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_031959270.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031815130.1 Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

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CloneID	OSa229308
Clone ID Related Accession (Same CDS sequence)	XM_031959270.1
Accession Version	XM_031959270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTT CCATAACTGA AGCAGATTTA ATTGGCAGCA GCTACGTTCG CATTACTAAA 
CTTTGCTGTG AACAGGGTGG GGCATATCCT GCAAAATATT CACAAGTTAA GGCAAATCTA 
GAACTGGATA TCTGCAAAAT CTGGTGTAGT AAGCCACTTC ATGTCCTTCG AGATTACTGT 
GATGTCATTA AAATATACAT CTTCTGGCCA CTTCTTTTTC AACGAGAACA GACTCCTGTA 
GTGTCTCAAT TACATCCTTG TATAGATGTC AGCAGCTCTA GTACTTGTGA ATTAAGTTTG 
AAGAGACTAC AGCATATTGA ATTGCTGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTA 
GATGATCAAT TTTTTATTGG TGGTATATTG AGAATTGGTT ATAAAATAGA AAATAAAATC 
TTGGCAATTG AGGAGGCTTT CAACTGGGTG AAATATACAG GGGAATTCAG AGTTTTGCGT 
AAATTGGAAA GAGCTGAAAA TTGTTGGCCT ATGCTAAGTA TTTTCTTTAC TGAATACAAG 
TACCATATAA CCAAAGTTGT CCTGGAAGAC TGTAATTTAG TTGAAGAATT TGAATATCAG 
AATTGTGCTG CTTGTATAAA GGAAGGTGAA ATTATGAAAA CAAGAGGAAA TGAAGAATTC 
TCAGAAGAGA GGTTTGATAC AGCTATCACA TATTATACCA GAGCAATTGA ATTTAGACCT 
GAAAACCATC TTCTTTATAG TAACCGAGCT CTTTGTTTCC TTAGGACTGG TCAATTCAAG 
AGTGCACTTG GTGATGGAAA GAGGGCCACT ATTCTGAAAT ACAATTGGCC AAAGGGCCAT 
TATCGTTTTT GCGATGCTCT TTCAATGTTG GGGGAGCATG CCTGGGCACT AGAAGCAAAT 
GAGAGAGCTC AACATCTTTG CCGAAACTAT CCAGATGGAA TGAAGGATCT TACTCAACAG 
AACATAAAAT TACAAAAACA AGTGGAAGAA TTAAGAGGTT CAAAACATGG TAGTCATCAG 
ATAAGGAAAT CGTTTTATGG AAAAAGGTTA TCCTCAGGTT ATAATTCTCC AAGACCTTTA 
CATTATGGTT TTCTTAATTT TATGGAAACT AATGAAGAAA ACAAGGTATT GAGATTAGCA 
TCGAAATATC GTGATTATTA TCATTATCAG AATTTGAAGG TATCTCGTGA AATTATGAAA 
TTAGAAAATA AAGACTATCC TCCAGATTTG CCACCAGGTC ATAATCCTAA ATTTAAAGGA 
AAATCAAAGA ATAAAAGCGA TGATGTAGGA AAACCAAGCC TACAACTGAA TTTACAAATA 
GATTTACAAA GCTTATTGGA CAAACAATTC AGTAAATCTT CCAGAGCTGT ACAACAGGAT 
TTTGCTATAT TGATAAAAAT GCTGCAGTCC CTCATCGAAG AAGGTTGTAC AGCTTTGATG 
GACCAACATT GTCGTAGTGC AGCCAAAGCT TTTTCACGGT TGTTAAGTGA TTTAGATCCC 
AATAAATTGA AGCAACTGAA CCTTGCCCTT ATTAATTATG TCCTAGTAAT CTATGGTTAT 
GCCATCTCTC TCCTTGGAAT AGGAATGCCT GAGGAATTAT CAGAGGCTGA ATATCAATTT 
AAGAGAATTA TTGAGCAATA CCCCAATGAA GGAGTTGATT GTTTAGCCTA TTTTGGAATT 
GGAAAAGTAT ATTTGAAAAA AAATAGATTT TCAGATGCAC TTGGTCATTT TCAAAAATCT 
AAAACTATGA TTAAGCTTAT TCCTGGAGTG TTGACTTGGC CTACAACTAA TGTGATTATT 
GAAGAGTCTC AAGCTGAGAA ATTGCAGTTG ATATTGGAAA ATCATATTGA AGAATGCAAA 
TTTCCACCAG ATCCAGATGC AGTTTGTTGT TATCAGAAGT GCAATGGATA TTCCAAAATC 
CAGATATATT TAAATGATCC AGACTTTAAG GGTTTTATAC GAATTAACTG CTGCCAGTTT 
TGTAGAATAG AATTTCATAT TAGCTGTTGG AAAAAATTAA AAACAACAAT TTTCAATGAT 
AAAAATGACA AGGATTTTCT CCAAGGAACA TGTCTTACTC CTGACTGTAG AGGTATTATT 
TCGAAGATTG TTATCTTTGG TGCAGCTGGC CATATTAAAT GTGAATTTGA ACATAGGGTC 
ACAAAAGAAA GGTATCCACC AAGACCTGTT ATGAAGCAGA AATGTTCTAG CTTAGAGAAA 
TTGAAAATTA AAGAAGAAAA AAAAGTAAAG AGAAAACTTC AAAAAAAAGA AGCACAAAAA 
ATAGCAAAAG AGAGAATGGA AGAGAAAACT AGAGAAAATA ATTCATCTAA GCATGGCGAC 
CATAAAGGAT TTGTACAAAG TTGCCATTTA CTTGATGATA GGATTTTGCT GTGTATAAAA 
CAAAATGCCG AGCAAATTAA ATCTGGCATC CAGGACGCTT CCAAACTTCT CAGAGAATTA 
CTTTCTTGGA AAGTAATAAA CACAGAGGAT TATGCAGATA CTTATTTTAA TAGCGGTTTT 
CTAAGTGAAA AAATGGAGCA TCTCATTGAC CATTTGGTAC GGAAAAACAA TAGAGTAAAT 
ACAAGGATTT TTCTGCATGT ACTGAGTGAG ATTGAAGAAA TAAATCCCAA ACTACGTGAC 
TGGATCCAGA AACTCAATAA TTTAGGTTTG GATGCTACAA GGACGTTTTT TTCTCGTTAT 
GGTGATTCTG TTAAAAAGCT TGATTTTAAA TTCATATCTA TGCTCTGGGT TGAAAAATAT 
GGTCATAAAC TAGATCACAT AATATCCAGT TATGAAGAGA GAAAGATAGT TGAATATTCC 
AATAATCAAG GATCATTGAA GGAAGCTCGT TATTTTATAT GGCTATTAGA AGAAAATAGA 
GAAAAGTTTC CAGCATTGCA TAATGCTTTA GATGAATTCT TTGATATAAT GGATGGTCCT 
TGCACAATAT TAAAGAAGCA AGAGACTGAA GATATTTCAT CTAATGGTAT TAAGGTAAAA 
AACAAAACCA GAAAAAAGAA GCACAAGGAG TCAAAGCCTG TTATCATACT CTCTGGAGTT 
GGTGGAGTAT CACAAAATGA TGAGAAAGTT GGTACAGATG AAAGTACTTT ACTTCAATCA 
AATTCTTATG ACCCATTTGT AGTACCCGAA TATCTTCGAG ATGAAGTAGA AGAATTTGAA 
GCTCTATATG AAAAAAATAG TTCAAATAAT TGTAATTATC AGAAGTTATT GGACAGTAAC 
TTAGATCCAA AATGTGAACC ATTATATGAC TATTTTTCTC AGATCCTGGA AGAACATGGT 
CCTTTGGAAA TTGATGACAA ATTATTGGTT GGAGAATATG AACATTTTCC TGAAGAAGCT 
AGGAAAATTG TGGAGGAAGC AGGAGGCTTA AAATCTTTTT TGTTAGATTC TCTTCGTTTT 
ATTATGATAG ACAATTATAT TGGATTGATG AAAGATGCTG TTCAACTTAA GAAAAATAAG 
ACCACTGGAA CTGATGGGGA AGAAAGTACT AGTTTGGATG AGAAAGACTC AATGATTTTC 
TCTCCACGCT TTCAGAAAAA TGCTTCCCAG CTCAAACTAC AGTTGAATCC AGCAGCTAAA 
GAATTTAAAC CAGTAACTTG TAATTCTTTC ATACCAGCAC CTAAAAATAA AAAAACAAAT 
AAGACTGCAA AGTATGTAAC AACAGGATAT GTGTCATATA TTCCTTTTAA TACAGTTTTT 
TCTAATCAAG GATTTGACAC CAGTAACACA TCAGTGTCTT ATGCAAATAT GTTGCCACAG 
TCATCTCAAT GCATTAGTTT ATATACAACT GTATCTGATA TATCTTCTGA TTATCCAAAA 
AAAAGAAAAC TACCATTGGT GTCAGCTGTA CCTCCTATGA CAGATAATGA CAAAAGAGAT 
AATTTTGACT CTGGCAATTC CGAGGCATGT AATGTAAAAT CTGAAAGACC AATGCTTGAA 
AAAGTATGTT CCTCTGGTAA ACCTAATTGT GAAAAAAAGA TCCGTCAGAA TACTCAGAGA 
ACAACTCTGA AGGCAGAAAT ATGTAAAAAT AATACTGCCA AAGATGCATG CCTTGGAAGT 
AATGATCATT GTGATCTTGG CACTAGTAAA CTAGAACGAG AGATGAAAAA TACGACTATC 
ATCAAAAATA CTCCATGTAT ACGAATGGTT GCCATACAGG TAAAAACTGA CTTAATGCAT 
CAGGAGGTTA ATACACTGCC TTTTGGTCCA TTTGAAACCC AACAAGGTGA TATTTTACGA 
ATGGAAAAGG AACATCAGGT GTTACAAGAG CAACTTAAAG AAGCAAGGGA AAAATATGAA 
CAGTTAAAAT GCAGAAGTAC AGAGGAAACC AGAGAGGTGG AAGAAAAACT GAAAAGAACA 
ATAGAAGAAA ATAAGATATC CAAGACTGAA TTGAATTGGT TCCATCAAGA TTTGGAAATA 
GAAGTGAAAA AATGGCAACA AGAAAAAAAG GAAAATCAAG AAAAATTGAA AACTTTAAGA 
AATAAAGTTA AAAAGCTTAA TGATACTCAT GAAATATATT CAAGAAATAA TGAAGAAAAG 
GATCAACAGT ATGCATCACA CTTGGATGAA TTTCTCAAAA TCAGTAATAA ATTTGCAAAT 
GAAAAATTGA AAATGGAGGA ACTTATAAAA AAGGGAAAAG AGAATTATGA GGAAACCATG 
AAAAGAGCTG TGGCTGCAGA GGTGTCCATA CTTGAAAATT GGAAGACTAC TGATGTGAGT 
AAGTTACAGA ACACTGCATC AAAAGCAGAA ATGTATGTGA AGAATCTAAA GTTAATGAAC 
AGTGATTCTG CATCATCTCT TAATTTGGAA TCAGAGATTA CTCACTGGGA ATCATTTATT 
TCAGAAATAA AAAAAGAAAT TGAGAAGGCA GAGTTTGAGT TTGAGGATCG AATTCAGAAA 
GTTAAAGAAG GTTCTCAACT GGATAAACTT TCTAAAGTAA AAGTTCCTGA ATTTCCATTA 
CCTACGGATA ATTTATTAAT TCAAGACAAG CCTGCTGATA TTAATGATCC AGCCTCTGTG 
ACATATTCTG CACAAGTTCA TTCTGACTTA TTTTCTGAAT ATGATGATCA AAGTCCAGTG 
ATTATCCCAG CTGACTTATT AACTGGAAGC CAAGCCCTGA CACTTCAGAA CACAAATCTA 
CATTCTGGCA GTGACTGCTC AGAGGAGATA ACTTCTGTAC TTTTACAAGC ATCTTATACT 
GATCCATGTC AGGTCACCCA GCCATCACCG ATTGTTAAGA GTTCTGAAAA GGATACTCAA 
ATTAATCAAA GTTCCAAAGG TGTTTCACAG ACACATGCCA GTACTCCAAA CCAGCCACCT 
TTTAACAATG TGATTGAGCA TCTGTCAACC ATATTCCCAA ATTACAGCAG TGTTGAGCTT 
TTGAATTTTA TCCAAAAAGT ACAAAATCAA GACAGAACTA CACATGCAAG TTTGGAGTCT 
GATGAAATTG TCAGAAGAGT AACAGAACTT ATTCTAGATG AGCAGAATAA AAAACTGGCA 
GTTCAAGGAA CAGACATGAG TGGTGCAGCT GAATCGTCTG ATCTTAGACA TTCAGCAGAA 
AATGTATCAG TATCTAGTCC CTCCATAGTC CCAGCTTCAG AAACACTTGC CAGCAAACCC 
TGGGTAGTTG TTGGAGGACC CTCCAAGTCA AAGTGGCAAA AATCACATGA TTCCTCTGTC 
TCCAGTGATG ATCCCTGCAT AATATGCTAT GAAGAGCTAA ACCAGGAACA TGTATGTGAG 
ATGGATTGTG GGCACCAGTT TCATAAATGG GCAACAGCTA CTGACCCTGA AATCCCACTG 
CTGATCAACA CAGAAGATCT GACCTACTCT GTTTCTGACT CGGGCCAGTT TACCCCTCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031815130.1
CDS	176..6058
Translation	METSITEADLIGSSYVRITKLCCEQGGAYPAKYSQVKANLELDICKIWCSKPLHVLRDYCDVIKIYIFWPLLFQREQTPVVSQLHPCIDVSSSSTCELSLKRLQHIELLEDIVDLAKKVVDDQFFIGGILRIGYKIENKILAIEEAFNWVKYTGEFRVLRKLERAENCWPMLSIFFTEYKYHITKVVLEDCNLVEEFEYQNCAACIKEGEIMKTRGNEEFSEERFDTAITYYTRAIEFRPENHLLYSNRALCFLRTGQFKSALGDGKRATILKYNWPKGHYRFCDALSMLGEHAWALEANERAQHLCRNYPDGMKDLTQQNIKLQKQVEELRGSKHGSHQIRKSFYGKRLSSGYNSPRPLHYGFLNFMETNEENKVLRLASKYRDYYHYQNLKVSREIMKLENKDYPPDLPPGHNPKFKGKSKNKSDDVGKPSLQLNLQIDLQSLLDKQFSKSSRAVQQDFAILIKMLQSLIEEGCTALMDQHCRSAAKAFSRLLSDLDPNKLKQLNLALINYVLVIYGYAISLLGIGMPEELSEAEYQFKRIIEQYPNEGVDCLAYFGIGKVYLKKNRFSDALGHFQKSKTMIKLIPGVLTWPTTNVIIEESQAEKLQLILENHIEECKFPPDPDAVCCYQKCNGYSKIQIYLNDPDFKGFIRINCCQFCRIEFHISCWKKLKTTIFNDKNDKDFLQGTCLTPDCRGIISKIVIFGAAGHIKCEFEHRVTKERYPPRPVMKQKCSSLEKLKIKEEKKVKRKLQKKEAQKIAKERMEEKTRENNSSKHGDHKGFVQSCHLLDDRILLCIKQNAEQIKSGIQDASKLLRELLSWKVINTEDYADTYFNSGFLSEKMEHLIDHLVRKNNRVNTRIFLHVLSEIEEINPKLRDWIQKLNNLGLDATRTFFSRYGDSVKKLDFKFISMLWVEKYGHKLDHIISSYEERKIVEYSNNQGSLKEARYFIWLLEENREKFPALHNALDEFFDIMDGPCTILKKQETEDISSNGIKVKNKTRKKKHKESKPVIILSGVGGVSQNDEKVGTDESTLLQSNSYDPFVVPEYLRDEVEEFEALYEKNSSNNCNYQKLLDSNLDPKCEPLYDYFSQILEEHGPLEIDDKLLVGEYEHFPEEARKIVEEAGGLKSFLLDSLRFIMIDNYIGLMKDAVQLKKNKTTGTDGEESTSLDEKDSMIFSPRFQKNASQLKLQLNPAAKEFKPVTCNSFIPAPKNKKTNKTAKYVTTGYVSYIPFNTVFSNQGFDTSNTSVSYANMLPQSSQCISLYTTVSDISSDYPKKRKLPLVSAVPPMTDNDKRDNFDSGNSEACNVKSERPMLEKVCSSGKPNCEKKIRQNTQRTTLKAEICKNNTAKDACLGSNDHCDLGTSKLEREMKNTTIIKNTPCIRMVAIQVKTDLMHQEVNTLPFGPFETQQGDILRMEKEHQVLQEQLKEAREKYEQLKCRSTEETREVEEKLKRTIEENKISKTELNWFHQDLEIEVKKWQQEKKENQEKLKTLRNKVKKLNDTHEIYSRNNEEKDQQYASHLDEFLKISNKFANEKLKMEELIKKGKENYEETMKRAVAAEVSILENWKTTDVSKLQNTASKAEMYVKNLKLMNSDSASSLNLESEITHWESFISEIKKEIEKAEFEFEDRIQKVKEGSQLDKLSKVKVPEFPLPTDNLLIQDKPADINDPASVTYSAQVHSDLFSEYDDQSPVIIPADLLTGSQALTLQNTNLHSGSDCSEEITSVLLQASYTDPCQVTQPSPIVKSSEKDTQINQSSKGVSQTHASTPNQPPFNNVIEHLSTIFPNYSSVELLNFIQKVQNQDRTTHASLESDEIVRRVTELILDEQNKKLAVQGTDMSGAAESSDLRHSAENVSVSSPSIVPASETLASKPWVVVGGPSKSKWQKSHDSSVSSDDPCIICYEELNQEHVCEMDCGHQFHKWATATDPEIPLLINTEDLTYSVSDSGQFTPP

Target ORF information:

RefSeq Version	XM_031959270.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031959270.1

ORF Insert Sequence:

ATGGAGACTT CCATAACTGA AGCAGATTTA ATTGGCAGCA GCTACGTTCG CATTACTAAA 
CTTTGCTGTG AACAGGGTGG GGCATATCCT GCAAAATATT CACAAGTTAA GGCAAATCTA 
GAACTGGATA TCTGCAAAAT CTGGTGTAGT AAGCCACTTC ATGTCCTTCG AGATTACTGT 
GATGTCATTA AAATATACAT CTTCTGGCCA CTTCTTTTTC AACGAGAACA GACTCCTGTA 
GTGTCTCAAT TACATCCTTG TATAGATGTC AGCAGCTCTA GTACTTGTGA ATTAAGTTTG 
AAGAGACTAC AGCATATTGA ATTGCTGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTA 
GATGATCAAT TTTTTATTGG TGGTATATTG AGAATTGGTT ATAAAATAGA AAATAAAATC 
TTGGCAATTG AGGAGGCTTT CAACTGGGTG AAATATACAG GGGAATTCAG AGTTTTGCGT 
AAATTGGAAA GAGCTGAAAA TTGTTGGCCT ATGCTAAGTA TTTTCTTTAC TGAATACAAG 
TACCATATAA CCAAAGTTGT CCTGGAAGAC TGTAATTTAG TTGAAGAATT TGAATATCAG 
AATTGTGCTG CTTGTATAAA GGAAGGTGAA ATTATGAAAA CAAGAGGAAA TGAAGAATTC 
TCAGAAGAGA GGTTTGATAC AGCTATCACA TATTATACCA GAGCAATTGA ATTTAGACCT 
GAAAACCATC TTCTTTATAG TAACCGAGCT CTTTGTTTCC TTAGGACTGG TCAATTCAAG 
AGTGCACTTG GTGATGGAAA GAGGGCCACT ATTCTGAAAT ACAATTGGCC AAAGGGCCAT 
TATCGTTTTT GCGATGCTCT TTCAATGTTG GGGGAGCATG CCTGGGCACT AGAAGCAAAT 
GAGAGAGCTC AACATCTTTG CCGAAACTAT CCAGATGGAA TGAAGGATCT TACTCAACAG 
AACATAAAAT TACAAAAACA AGTGGAAGAA TTAAGAGGTT CAAAACATGG TAGTCATCAG 
ATAAGGAAAT CGTTTTATGG AAAAAGGTTA TCCTCAGGTT ATAATTCTCC AAGACCTTTA 
CATTATGGTT TTCTTAATTT TATGGAAACT AATGAAGAAA ACAAGGTATT GAGATTAGCA 
TCGAAATATC GTGATTATTA TCATTATCAG AATTTGAAGG TATCTCGTGA AATTATGAAA 
TTAGAAAATA AAGACTATCC TCCAGATTTG CCACCAGGTC ATAATCCTAA ATTTAAAGGA 
AAATCAAAGA ATAAAAGCGA TGATGTAGGA AAACCAAGCC TACAACTGAA TTTACAAATA 
GATTTACAAA GCTTATTGGA CAAACAATTC AGTAAATCTT CCAGAGCTGT ACAACAGGAT 
TTTGCTATAT TGATAAAAAT GCTGCAGTCC CTCATCGAAG AAGGTTGTAC AGCTTTGATG 
GACCAACATT GTCGTAGTGC AGCCAAAGCT TTTTCACGGT TGTTAAGTGA TTTAGATCCC 
AATAAATTGA AGCAACTGAA CCTTGCCCTT ATTAATTATG TCCTAGTAAT CTATGGTTAT 
GCCATCTCTC TCCTTGGAAT AGGAATGCCT GAGGAATTAT CAGAGGCTGA ATATCAATTT 
AAGAGAATTA TTGAGCAATA CCCCAATGAA GGAGTTGATT GTTTAGCCTA TTTTGGAATT 
GGAAAAGTAT ATTTGAAAAA AAATAGATTT TCAGATGCAC TTGGTCATTT TCAAAAATCT 
AAAACTATGA TTAAGCTTAT TCCTGGAGTG TTGACTTGGC CTACAACTAA TGTGATTATT 
GAAGAGTCTC AAGCTGAGAA ATTGCAGTTG ATATTGGAAA ATCATATTGA AGAATGCAAA 
TTTCCACCAG ATCCAGATGC AGTTTGTTGT TATCAGAAGT GCAATGGATA TTCCAAAATC 
CAGATATATT TAAATGATCC AGACTTTAAG GGTTTTATAC GAATTAACTG CTGCCAGTTT 
TGTAGAATAG AATTTCATAT TAGCTGTTGG AAAAAATTAA AAACAACAAT TTTCAATGAT 
AAAAATGACA AGGATTTTCT CCAAGGAACA TGTCTTACTC CTGACTGTAG AGGTATTATT 
TCGAAGATTG TTATCTTTGG TGCAGCTGGC CATATTAAAT GTGAATTTGA ACATAGGGTC 
ACAAAAGAAA GGTATCCACC AAGACCTGTT ATGAAGCAGA AATGTTCTAG CTTAGAGAAA 
TTGAAAATTA AAGAAGAAAA AAAAGTAAAG AGAAAACTTC AAAAAAAAGA AGCACAAAAA 
ATAGCAAAAG AGAGAATGGA AGAGAAAACT AGAGAAAATA ATTCATCTAA GCATGGCGAC 
CATAAAGGAT TTGTACAAAG TTGCCATTTA CTTGATGATA GGATTTTGCT GTGTATAAAA 
CAAAATGCCG AGCAAATTAA ATCTGGCATC CAGGACGCTT CCAAACTTCT CAGAGAATTA 
CTTTCTTGGA AAGTAATAAA CACAGAGGAT TATGCAGATA CTTATTTTAA TAGCGGTTTT 
CTAAGTGAAA AAATGGAGCA TCTCATTGAC CATTTGGTAC GGAAAAACAA TAGAGTAAAT 
ACAAGGATTT TTCTGCATGT ACTGAGTGAG ATTGAAGAAA TAAATCCCAA ACTACGTGAC 
TGGATCCAGA AACTCAATAA TTTAGGTTTG GATGCTACAA GGACGTTTTT TTCTCGTTAT 
GGTGATTCTG TTAAAAAGCT TGATTTTAAA TTCATATCTA TGCTCTGGGT TGAAAAATAT 
GGTCATAAAC TAGATCACAT AATATCCAGT TATGAAGAGA GAAAGATAGT TGAATATTCC 
AATAATCAAG GATCATTGAA GGAAGCTCGT TATTTTATAT GGCTATTAGA AGAAAATAGA 
GAAAAGTTTC CAGCATTGCA TAATGCTTTA GATGAATTCT TTGATATAAT GGATGGTCCT 
TGCACAATAT TAAAGAAGCA AGAGACTGAA GATATTTCAT CTAATGGTAT TAAGGTAAAA 
AACAAAACCA GAAAAAAGAA GCACAAGGAG TCAAAGCCTG TTATCATACT CTCTGGAGTT 
GGTGGAGTAT CACAAAATGA TGAGAAAGTT GGTACAGATG AAAGTACTTT ACTTCAATCA 
AATTCTTATG ACCCATTTGT AGTACCCGAA TATCTTCGAG ATGAAGTAGA AGAATTTGAA 
GCTCTATATG AAAAAAATAG TTCAAATAAT TGTAATTATC AGAAGTTATT GGACAGTAAC 
TTAGATCCAA AATGTGAACC ATTATATGAC TATTTTTCTC AGATCCTGGA AGAACATGGT 
CCTTTGGAAA TTGATGACAA ATTATTGGTT GGAGAATATG AACATTTTCC TGAAGAAGCT 
AGGAAAATTG TGGAGGAAGC AGGAGGCTTA AAATCTTTTT TGTTAGATTC TCTTCGTTTT 
ATTATGATAG ACAATTATAT TGGATTGATG AAAGATGCTG TTCAACTTAA GAAAAATAAG 
ACCACTGGAA CTGATGGGGA AGAAAGTACT AGTTTGGATG AGAAAGACTC AATGATTTTC 
TCTCCACGCT TTCAGAAAAA TGCTTCCCAG CTCAAACTAC AGTTGAATCC AGCAGCTAAA 
GAATTTAAAC CAGTAACTTG TAATTCTTTC ATACCAGCAC CTAAAAATAA AAAAACAAAT 
AAGACTGCAA AGTATGTAAC AACAGGATAT GTGTCATATA TTCCTTTTAA TACAGTTTTT 
TCTAATCAAG GATTTGACAC CAGTAACACA TCAGTGTCTT ATGCAAATAT GTTGCCACAG 
TCATCTCAAT GCATTAGTTT ATATACAACT GTATCTGATA TATCTTCTGA TTATCCAAAA 
AAAAGAAAAC TACCATTGGT GTCAGCTGTA CCTCCTATGA CAGATAATGA CAAAAGAGAT 
AATTTTGACT CTGGCAATTC CGAGGCATGT AATGTAAAAT CTGAAAGACC AATGCTTGAA 
AAAGTATGTT CCTCTGGTAA ACCTAATTGT GAAAAAAAGA TCCGTCAGAA TACTCAGAGA 
ACAACTCTGA AGGCAGAAAT ATGTAAAAAT AATACTGCCA AAGATGCATG CCTTGGAAGT 
AATGATCATT GTGATCTTGG CACTAGTAAA CTAGAACGAG AGATGAAAAA TACGACTATC 
ATCAAAAATA CTCCATGTAT ACGAATGGTT GCCATACAGG TAAAAACTGA CTTAATGCAT 
CAGGAGGTTA ATACACTGCC TTTTGGTCCA TTTGAAACCC AACAAGGTGA TATTTTACGA 
ATGGAAAAGG AACATCAGGT GTTACAAGAG CAACTTAAAG AAGCAAGGGA AAAATATGAA 
CAGTTAAAAT GCAGAAGTAC AGAGGAAACC AGAGAGGTGG AAGAAAAACT GAAAAGAACA 
ATAGAAGAAA ATAAGATATC CAAGACTGAA TTGAATTGGT TCCATCAAGA TTTGGAAATA 
GAAGTGAAAA AATGGCAACA AGAAAAAAAG GAAAATCAAG AAAAATTGAA AACTTTAAGA 
AATAAAGTTA AAAAGCTTAA TGATACTCAT GAAATATATT CAAGAAATAA TGAAGAAAAG 
GATCAACAGT ATGCATCACA CTTGGATGAA TTTCTCAAAA TCAGTAATAA ATTTGCAAAT 
GAAAAATTGA AAATGGAGGA ACTTATAAAA AAGGGAAAAG AGAATTATGA GGAAACCATG 
AAAAGAGCTG TGGCTGCAGA GGTGTCCATA CTTGAAAATT GGAAGACTAC TGATGTGAGT 
AAGTTACAGA ACACTGCATC AAAAGCAGAA ATGTATGTGA AGAATCTAAA GTTAATGAAC 
AGTGATTCTG CATCATCTCT TAATTTGGAA TCAGAGATTA CTCACTGGGA ATCATTTATT 
TCAGAAATAA AAAAAGAAAT TGAGAAGGCA GAGTTTGAGT TTGAGGATCG AATTCAGAAA 
GTTAAAGAAG GTTCTCAACT GGATAAACTT TCTAAAGTAA AAGTTCCTGA ATTTCCATTA 
CCTACGGATA ATTTATTAAT TCAAGACAAG CCTGCTGATA TTAATGATCC AGCCTCTGTG 
ACATATTCTG CACAAGTTCA TTCTGACTTA TTTTCTGAAT ATGATGATCA AAGTCCAGTG 
ATTATCCCAG CTGACTTATT AACTGGAAGC CAAGCCCTGA CACTTCAGAA CACAAATCTA 
CATTCTGGCA GTGACTGCTC AGAGGAGATA ACTTCTGTAC TTTTACAAGC ATCTTATACT 
GATCCATGTC AGGTCACCCA GCCATCACCG ATTGTTAAGA GTTCTGAAAA GGATACTCAA 
ATTAATCAAA GTTCCAAAGG TGTTTCACAG ACACATGCCA GTACTCCAAA CCAGCCACCT 
TTTAACAATG TGATTGAGCA TCTGTCAACC ATATTCCCAA ATTACAGCAG TGTTGAGCTT 
TTGAATTTTA TCCAAAAAGT ACAAAATCAA GACAGAACTA CACATGCAAG TTTGGAGTCT 
GATGAAATTG TCAGAAGAGT AACAGAACTT ATTCTAGATG AGCAGAATAA AAAACTGGCA 
GTTCAAGGAA CAGACATGAG TGGTGCAGCT GAATCGTCTG ATCTTAGACA TTCAGCAGAA 
AATGTATCAG TATCTAGTCC CTCCATAGTC CCAGCTTCAG AAACACTTGC CAGCAAACCC 
TGGGTAGTTG TTGGAGGACC CTCCAAGTCA AAGTGGCAAA AATCACATGA TTCCTCTGTC 
TCCAGTGATG ATCCCTGCAT AATATGCTAT GAAGAGCTAA ACCAGGAACA TGTATGTGAG 
ATGGATTGTG GGCACCAGTT TCATAAATGG GCAACAGCTA CTGACCCTGA AATCCCACTG 
CTGATCAACA CAGAAGATCT GACCTACTCT GTTTCTGACT CGGGCCAGTT TACCCCTCCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_031959271.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031815131.1 Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

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CloneID	OSa229309
Clone ID Related Accession (Same CDS sequence)	XM_031959271.1
Accession Version	XM_031959271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase TTC3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTT CCATAACTGA AGCAGATTTA ATTGGCAGCA GCTACGTTCG CATTACTAAA 
CTTTGCTGTG AACAGGGTGG GGCATATCCT GCAAAATATT CACAAGTTAA GGCAAATCTA 
GAACTGGATA TCTGCAAAAT CTGGTGTAGT AAGCCACTTC ATGTCCTTCG AGATTACTGT 
GATGTCATTA AAATATACAT CTTCTGGCCA CTTCTTTTTC AACGAGAACA GACTCCTGTA 
GTGTCTCAAT TACATCCTTG TATAGATGTC AGCAGCTCTA GTACTTGTGA ATTAAGTTTG 
AAGAGACTAC AGCATATTGA ATTGCTGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTA 
GATGATCAAT TTTTTATTGG TGGTATATTG AGAATTGGTT ATAAAATAGA AAATAAAATC 
TTGGCAATTG AGGAGGCTTT CAACTGGGTG AAATATACAG GGGAATTCAG AGTTTTGCGT 
AAATTGGAAA GAGCTGAAAA TTGTTGGCCT ATGCTAAGTA TTTTCTTTAC TGAATACAAG 
TACCATATAA CCAAAGTTGT CCTGGAAGAC TGTAATTTAG TTGAAGAATT TGAATATCAG 
AATTGTGCTG CTTGTATAAA GGAAGGTGAA ATTATGAAAA CAAGAGGAAA TGAAGAATTC 
TCAGAAGAGA GGTTTGATAC AGCTATCACA TATTATACCA GAGCAATTGA ATTTAGACCT 
GAAAACCATC TTCTTTATAG TAACCGAGCT CTTTGTTTCC TTAGGACTGG TCAATTCAAG 
AGTGCACTTG GTGATGGAAA GAGGGCCACT ATTCTGAAAT ACAATTGGCC AAAGGGCCAT 
TATCGTTTTT GCGATGCTCT TTCAATGTTG GGGGAGCATG CCTGGGCACT AGAAGCAAAT 
GAGAGAGCTC AACATCTTTG CCGAAACTAT CCAGATGGAA TGAAGGATCT TACTCAACAG 
AACATAAAAT TACAAAAACA AGTGGAAGAA TTAAGAGGTT CAAAACATGG TAGTCATCAG 
ATAAGGAAAT CGTTTTATGG AAAAAGGTTA TCCTCAGGTT ATAATTCTCC AAGACCTTTA 
CATTATGGTT TTCTTAATTT TATGGAAACT AATGAAGAAA ACAAGGTATC TCGTGAAATT 
ATGAAATTAG AAAATAAAGA CTATCCTCCA GATTTGCCAC CAGGTCATAA TCCTAAATTT 
AAAGGAAAAT CAAAGAATAA AAGCGATGAT GTAGGAAAAC CAAGCCTACA ACTGAATTTA 
CAAATAGATT TACAAAGCTT ATTGGACAAA CAATTCAGTA AATCTTCCAG AGCTGTACAA 
CAGGATTTTG CTATATTGAT AAAAATGCTG CAGTCCCTCA TCGAAGAAGG TTGTACAGCT 
TTGATGGACC AACATTGTCG TAGTGCAGCC AAAGCTTTTT CACGGTTGTT AAGTGATTTA 
GATCCCAATA AATTGAAGCA ACTGAACCTT GCCCTTATTA ATTATGTCCT AGTAATCTAT 
GGTTATGCCA TCTCTCTCCT TGGAATAGGA ATGCCTGAGG AATTATCAGA GGCTGAATAT 
CAATTTAAGA GAATTATTGA GCAATACCCC AATGAAGGAG TTGATTGTTT AGCCTATTTT 
GGAATTGGAA AAGTATATTT GAAAAAAAAT AGATTTTCAG ATGCACTTGG TCATTTTCAA 
AAATCTAAAA CTATGATTAA GCTTATTCCT GGAGTGTTGA CTTGGCCTAC AACTAATGTG 
ATTATTGAAG AGTCTCAAGC TGAGAAATTG CAGTTGATAT TGGAAAATCA TATTGAAGAA 
TGCAAATTTC CACCAGATCC AGATGCAGTT TGTTGTTATC AGAAGTGCAA TGGATATTCC 
AAAATCCAGA TATATTTAAA TGATCCAGAC TTTAAGGGTT TTATACGAAT TAACTGCTGC 
CAGTTTTGTA GAATAGAATT TCATATTAGC TGTTGGAAAA AATTAAAAAC AACAATTTTC 
AATGATAAAA ATGACAAGGA TTTTCTCCAA GGAACATGTC TTACTCCTGA CTGTAGAGGT 
ATTATTTCGA AGATTGTTAT CTTTGGTGCA GCTGGCCATA TTAAATGTGA ATTTGAACAT 
AGGGTCACAA AAGAAAGGTA TCCACCAAGA CCTGTTATGA AGCAGAAATG TTCTAGCTTA 
GAGAAATTGA AAATTAAAGA AGAAAAAAAA GTAAAGAGAA AACTTCAAAA AAAAGAAGCA 
CAAAAAATAG CAAAAGAGAG AATGGAAGAG AAAACTAGAG AAAATAATTC ATCTAAGCAT 
GGCGACCATA AAGGATTTGT ACAAAGTTGC CATTTACTTG ATGATAGGAT TTTGCTGTGT 
ATAAAACAAA ATGCCGAGCA AATTAAATCT GGCATCCAGG ACGCTTCCAA ACTTCTCAGA 
GAATTACTTT CTTGGAAAGT AATAAACACA GAGGATTATG CAGATACTTA TTTTAATAGC 
GGTTTTCTAA GTGAAAAAAT GGAGCATCTC ATTGACCATT TGGTACGGAA AAACAATAGA 
GTAAATACAA GGATTTTTCT GCATGTACTG AGTGAGATTG AAGAAATAAA TCCCAAACTA 
CGTGACTGGA TCCAGAAACT CAATAATTTA GGTTTGGATG CTACAAGGAC GTTTTTTTCT 
CGTTATGGTG ATTCTGTTAA AAAGCTTGAT TTTAAATTCA TATCTATGCT CTGGGTTGAA 
AAATATGGTC ATAAACTAGA TCACATAATA TCCAGTTATG AAGAGAGAAA GATAGTTGAA 
TATTCCAATA ATCAAGGATC ATTGAAGGAA GCTCGTTATT TTATATGGCT ATTAGAAGAA 
AATAGAGAAA AGTTTCCAGC ATTGCATAAT GCTTTAGATG AATTCTTTGA TATAATGGAT 
GGTCCTTGCA CAATATTAAA GAAGCAAGAG ACTGAAGATA TTTCATCTAA TGGTATTAAG 
GTAAAAAACA AAACCAGAAA AAAGAAGCAC AAGGAGTCAA AGCCTGTTAT CATACTCTCT 
GGAGTTGGTG GAGTATCACA AAATGATGAG AAAGTTGGTA CAGATGAAAG TACTTTACTT 
CAATCAAATT CTTATGACCC ATTTGTAGTA CCCGAATATC TTCGAGATGA AGTAGAAGAA 
TTTGAAGCTC TATATGAAAA AAATAGTTCA AATAATTGTA ATTATCAGAA GTTATTGGAC 
AGTAACTTAG ATCCAAAATG TGAACCATTA TATGACTATT TTTCTCAGAT CCTGGAAGAA 
CATGGTCCTT TGGAAATTGA TGACAAATTA TTGGTTGGAG AATATGAACA TTTTCCTGAA 
GAAGCTAGGA AAATTGTGGA GGAAGCAGGA GGCTTAAAAT CTTTTTTGTT AGATTCTCTT 
CGTTTTATTA TGATAGACAA TTATATTGGA TTGATGAAAG ATGCTGTTCA ACTTAAGAAA 
AATAAGACCA CTGGAACTGA TGGGGAAGAA AGTACTAGTT TGGATGAGAA AGACTCAATG 
ATTTTCTCTC CACGCTTTCA GAAAAATGCT TCCCAGCTCA AACTACAGTT GAATCCAGCA 
GCTAAAGAAT TTAAACCAGT AACTTGTAAT TCTTTCATAC CAGCACCTAA AAATAAAAAA 
ACAAATAAGA CTGCAAAGTA TGTAACAACA GGATATGTGT CATATATTCC TTTTAATACA 
GTTTTTTCTA ATCAAGGATT TGACACCAGT AACACATCAG TGTCTTATGC AAATATGTTG 
CCACAGTCAT CTCAATGCAT TAGTTTATAT ACAACTGTAT CTGATATATC TTCTGATTAT 
CCAAAAAAAA GAAAACTACC ATTGGTGTCA GCTGTACCTC CTATGACAGA TAATGACAAA 
AGAGATAATT TTGACTCTGG CAATTCCGAG GCATGTAATG TAAAATCTGA AAGACCAATG 
CTTGAAAAAG TATGTTCCTC TGGTAAACCT AATTGTGAAA AAAAGATCCG TCAGAATACT 
CAGAGAACAA CTCTGAAGGC AGAAATATGT AAAAATAATA CTGCCAAAGA TGCATGCCTT 
GGAAGTAATG ATCATTGTGA TCTTGGCACT AGTAAACTAG AACGAGAGAT GAAAAATACG 
ACTATCATCA AAAATACTCC ATGTATACGA ATGGTTGCCA TACAGGTAAA AACTGACTTA 
ATGCATCAGG AGGTTAATAC ACTGCCTTTT GGTCCATTTG AAACCCAACA AGGTGATATT 
TTACGAATGG AAAAGGAACA TCAGGTGTTA CAAGAGCAAC TTAAAGAAGC AAGGGAAAAA 
TATGAACAGT TAAAATGCAG AAGTACAGAG GAAACCAGAG AGGTGGAAGA AAAACTGAAA 
AGAACAATAG AAGAAAATAA GATATCCAAG ACTGAATTGA ATTGGTTCCA TCAAGATTTG 
GAAATAGAAG TGAAAAAATG GCAACAAGAA AAAAAGGAAA ATCAAGAAAA ATTGAAAACT 
TTAAGAAATA AAGTTAAAAA GCTTAATGAT ACTCATGAAA TATATTCAAG AAATAATGAA 
GAAAAGGATC AACAGTATGC ATCACACTTG GATGAATTTC TCAAAATCAG TAATAAATTT 
GCAAATGAAA AATTGAAAAT GGAGGAACTT ATAAAAAAGG GAAAAGAGAA TTATGAGGAA 
ACCATGAAAA GAGCTGTGGC TGCAGAGGTG TCCATACTTG AAAATTGGAA GACTACTGAT 
GTGAGTAAGT TACAGAACAC TGCATCAAAA GCAGAAATGT ATGTGAAGAA TCTAAAGTTA 
ATGAACAGTG ATTCTGCATC ATCTCTTAAT TTGGAATCAG AGATTACTCA CTGGGAATCA 
TTTATTTCAG AAATAAAAAA AGAAATTGAG AAGGCAGAGT TTGAGTTTGA GGATCGAATT 
CAGAAAGTTA AAGAAGGTTC TCAACTGGAT AAACTTTCTA AAGTAAAAGT TCCTGAATTT 
CCATTACCTA CGGATAATTT ATTAATTCAA GACAAGCCTG CTGATATTAA TGATCCAGCC 
TCTGTGACAT ATTCTGCACA AGTTCATTCT GACTTATTTT CTGAATATGA TGATCAAAGT 
CCAGTGATTA TCCCAGCTGA CTTATTAACT GGAAGCCAAG CCCTGACACT TCAGAACACA 
AATCTACATT CTGGCAGTGA CTGCTCAGAG GAGATAACTT CTGTACTTTT ACAAGCATCT 
TATACTGATC CATGTCAGGT CACCCAGCCA TCACCGATTG TTAAGAGTTC TGAAAAGGAT 
ACTCAAATTA ATCAAAGTTC CAAAGGTGTT TCACAGACAC ATGCCAGTAC TCCAAACCAG 
CCACCTTTTA ACAATGTGAT TGAGCATCTG TCAACCATAT TCCCAAATTA CAGCAGTGTT 
GAGCTTTTGA ATTTTATCCA AAAAGTACAA AATCAAGACA GAACTACACA TGCAAGTTTG 
GAGTCTGATG AAATTGTCAG AAGAGTAACA GAACTTATTC TAGATGAGCA GAATAAAAAA 
CTGGCAGTTC AAGGAACAGA CATGAGTGGT GCAGCTGAAT CGTCTGATCT TAGACATTCA 
GCAGAAAATG TATCAGTATC TAGTCCCTCC ATAGTCCCAG CTTCAGAAAC ACTTGCCAGC 
AAACCCTGGG TAGTTGTTGG AGGACCCTCC AAGTCAAAGT GGCAAAAATC ACATGATTCC 
TCTGTCTCCA GTGATGATCC CTGCATAATA TGCTATGAAG AGCTAAACCA GGAACATGTA 
TGTGAGATGG ATTGTGGGCA CCAGTTTCAT AAATGGTGCA TTGAGCAGTG GCTTAAAGAA 
CAAAGTACAT GCCCAACATG CCGTGAATAT GTCCTGTTAG CAGAAGAATT TCCTGCACTT 
TCTGGATCAG GCCGGCCTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031815131.1
CDS	175..6018
Translation	METSITEADLIGSSYVRITKLCCEQGGAYPAKYSQVKANLELDICKIWCSKPLHVLRDYCDVIKIYIFWPLLFQREQTPVVSQLHPCIDVSSSSTCELSLKRLQHIELLEDIVDLAKKVVDDQFFIGGILRIGYKIENKILAIEEAFNWVKYTGEFRVLRKLERAENCWPMLSIFFTEYKYHITKVVLEDCNLVEEFEYQNCAACIKEGEIMKTRGNEEFSEERFDTAITYYTRAIEFRPENHLLYSNRALCFLRTGQFKSALGDGKRATILKYNWPKGHYRFCDALSMLGEHAWALEANERAQHLCRNYPDGMKDLTQQNIKLQKQVEELRGSKHGSHQIRKSFYGKRLSSGYNSPRPLHYGFLNFMETNEENKVSREIMKLENKDYPPDLPPGHNPKFKGKSKNKSDDVGKPSLQLNLQIDLQSLLDKQFSKSSRAVQQDFAILIKMLQSLIEEGCTALMDQHCRSAAKAFSRLLSDLDPNKLKQLNLALINYVLVIYGYAISLLGIGMPEELSEAEYQFKRIIEQYPNEGVDCLAYFGIGKVYLKKNRFSDALGHFQKSKTMIKLIPGVLTWPTTNVIIEESQAEKLQLILENHIEECKFPPDPDAVCCYQKCNGYSKIQIYLNDPDFKGFIRINCCQFCRIEFHISCWKKLKTTIFNDKNDKDFLQGTCLTPDCRGIISKIVIFGAAGHIKCEFEHRVTKERYPPRPVMKQKCSSLEKLKIKEEKKVKRKLQKKEAQKIAKERMEEKTRENNSSKHGDHKGFVQSCHLLDDRILLCIKQNAEQIKSGIQDASKLLRELLSWKVINTEDYADTYFNSGFLSEKMEHLIDHLVRKNNRVNTRIFLHVLSEIEEINPKLRDWIQKLNNLGLDATRTFFSRYGDSVKKLDFKFISMLWVEKYGHKLDHIISSYEERKIVEYSNNQGSLKEARYFIWLLEENREKFPALHNALDEFFDIMDGPCTILKKQETEDISSNGIKVKNKTRKKKHKESKPVIILSGVGGVSQNDEKVGTDESTLLQSNSYDPFVVPEYLRDEVEEFEALYEKNSSNNCNYQKLLDSNLDPKCEPLYDYFSQILEEHGPLEIDDKLLVGEYEHFPEEARKIVEEAGGLKSFLLDSLRFIMIDNYIGLMKDAVQLKKNKTTGTDGEESTSLDEKDSMIFSPRFQKNASQLKLQLNPAAKEFKPVTCNSFIPAPKNKKTNKTAKYVTTGYVSYIPFNTVFSNQGFDTSNTSVSYANMLPQSSQCISLYTTVSDISSDYPKKRKLPLVSAVPPMTDNDKRDNFDSGNSEACNVKSERPMLEKVCSSGKPNCEKKIRQNTQRTTLKAEICKNNTAKDACLGSNDHCDLGTSKLEREMKNTTIIKNTPCIRMVAIQVKTDLMHQEVNTLPFGPFETQQGDILRMEKEHQVLQEQLKEAREKYEQLKCRSTEETREVEEKLKRTIEENKISKTELNWFHQDLEIEVKKWQQEKKENQEKLKTLRNKVKKLNDTHEIYSRNNEEKDQQYASHLDEFLKISNKFANEKLKMEELIKKGKENYEETMKRAVAAEVSILENWKTTDVSKLQNTASKAEMYVKNLKLMNSDSASSLNLESEITHWESFISEIKKEIEKAEFEFEDRIQKVKEGSQLDKLSKVKVPEFPLPTDNLLIQDKPADINDPASVTYSAQVHSDLFSEYDDQSPVIIPADLLTGSQALTLQNTNLHSGSDCSEEITSVLLQASYTDPCQVTQPSPIVKSSEKDTQINQSSKGVSQTHASTPNQPPFNNVIEHLSTIFPNYSSVELLNFIQKVQNQDRTTHASLESDEIVRRVTELILDEQNKKLAVQGTDMSGAAESSDLRHSAENVSVSSPSIVPASETLASKPWVVVGGPSKSKWQKSHDSSVSSDDPCIICYEELNQEHVCEMDCGHQFHKWCIEQWLKEQSTCPTCREYVLLAEEFPALSGSGRPA

Target ORF information:

RefSeq Version	XM_031959271.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031959271.1

ORF Insert Sequence:

ATGGAGACTT CCATAACTGA AGCAGATTTA ATTGGCAGCA GCTACGTTCG CATTACTAAA 
CTTTGCTGTG AACAGGGTGG GGCATATCCT GCAAAATATT CACAAGTTAA GGCAAATCTA 
GAACTGGATA TCTGCAAAAT CTGGTGTAGT AAGCCACTTC ATGTCCTTCG AGATTACTGT 
GATGTCATTA AAATATACAT CTTCTGGCCA CTTCTTTTTC AACGAGAACA GACTCCTGTA 
GTGTCTCAAT TACATCCTTG TATAGATGTC AGCAGCTCTA GTACTTGTGA ATTAAGTTTG 
AAGAGACTAC AGCATATTGA ATTGCTGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTA 
GATGATCAAT TTTTTATTGG TGGTATATTG AGAATTGGTT ATAAAATAGA AAATAAAATC 
TTGGCAATTG AGGAGGCTTT CAACTGGGTG AAATATACAG GGGAATTCAG AGTTTTGCGT 
AAATTGGAAA GAGCTGAAAA TTGTTGGCCT ATGCTAAGTA TTTTCTTTAC TGAATACAAG 
TACCATATAA CCAAAGTTGT CCTGGAAGAC TGTAATTTAG TTGAAGAATT TGAATATCAG 
AATTGTGCTG CTTGTATAAA GGAAGGTGAA ATTATGAAAA CAAGAGGAAA TGAAGAATTC 
TCAGAAGAGA GGTTTGATAC AGCTATCACA TATTATACCA GAGCAATTGA ATTTAGACCT 
GAAAACCATC TTCTTTATAG TAACCGAGCT CTTTGTTTCC TTAGGACTGG TCAATTCAAG 
AGTGCACTTG GTGATGGAAA GAGGGCCACT ATTCTGAAAT ACAATTGGCC AAAGGGCCAT 
TATCGTTTTT GCGATGCTCT TTCAATGTTG GGGGAGCATG CCTGGGCACT AGAAGCAAAT 
GAGAGAGCTC AACATCTTTG CCGAAACTAT CCAGATGGAA TGAAGGATCT TACTCAACAG 
AACATAAAAT TACAAAAACA AGTGGAAGAA TTAAGAGGTT CAAAACATGG TAGTCATCAG 
ATAAGGAAAT CGTTTTATGG AAAAAGGTTA TCCTCAGGTT ATAATTCTCC AAGACCTTTA 
CATTATGGTT TTCTTAATTT TATGGAAACT AATGAAGAAA ACAAGGTATC TCGTGAAATT 
ATGAAATTAG AAAATAAAGA CTATCCTCCA GATTTGCCAC CAGGTCATAA TCCTAAATTT 
AAAGGAAAAT CAAAGAATAA AAGCGATGAT GTAGGAAAAC CAAGCCTACA ACTGAATTTA 
CAAATAGATT TACAAAGCTT ATTGGACAAA CAATTCAGTA AATCTTCCAG AGCTGTACAA 
CAGGATTTTG CTATATTGAT AAAAATGCTG CAGTCCCTCA TCGAAGAAGG TTGTACAGCT 
TTGATGGACC AACATTGTCG TAGTGCAGCC AAAGCTTTTT CACGGTTGTT AAGTGATTTA 
GATCCCAATA AATTGAAGCA ACTGAACCTT GCCCTTATTA ATTATGTCCT AGTAATCTAT 
GGTTATGCCA TCTCTCTCCT TGGAATAGGA ATGCCTGAGG AATTATCAGA GGCTGAATAT 
CAATTTAAGA GAATTATTGA GCAATACCCC AATGAAGGAG TTGATTGTTT AGCCTATTTT 
GGAATTGGAA AAGTATATTT GAAAAAAAAT AGATTTTCAG ATGCACTTGG TCATTTTCAA 
AAATCTAAAA CTATGATTAA GCTTATTCCT GGAGTGTTGA CTTGGCCTAC AACTAATGTG 
ATTATTGAAG AGTCTCAAGC TGAGAAATTG CAGTTGATAT TGGAAAATCA TATTGAAGAA 
TGCAAATTTC CACCAGATCC AGATGCAGTT TGTTGTTATC AGAAGTGCAA TGGATATTCC 
AAAATCCAGA TATATTTAAA TGATCCAGAC TTTAAGGGTT TTATACGAAT TAACTGCTGC 
CAGTTTTGTA GAATAGAATT TCATATTAGC TGTTGGAAAA AATTAAAAAC AACAATTTTC 
AATGATAAAA ATGACAAGGA TTTTCTCCAA GGAACATGTC TTACTCCTGA CTGTAGAGGT 
ATTATTTCGA AGATTGTTAT CTTTGGTGCA GCTGGCCATA TTAAATGTGA ATTTGAACAT 
AGGGTCACAA AAGAAAGGTA TCCACCAAGA CCTGTTATGA AGCAGAAATG TTCTAGCTTA 
GAGAAATTGA AAATTAAAGA AGAAAAAAAA GTAAAGAGAA AACTTCAAAA AAAAGAAGCA 
CAAAAAATAG CAAAAGAGAG AATGGAAGAG AAAACTAGAG AAAATAATTC ATCTAAGCAT 
GGCGACCATA AAGGATTTGT ACAAAGTTGC CATTTACTTG ATGATAGGAT TTTGCTGTGT 
ATAAAACAAA ATGCCGAGCA AATTAAATCT GGCATCCAGG ACGCTTCCAA ACTTCTCAGA 
GAATTACTTT CTTGGAAAGT AATAAACACA GAGGATTATG CAGATACTTA TTTTAATAGC 
GGTTTTCTAA GTGAAAAAAT GGAGCATCTC ATTGACCATT TGGTACGGAA AAACAATAGA 
GTAAATACAA GGATTTTTCT GCATGTACTG AGTGAGATTG AAGAAATAAA TCCCAAACTA 
CGTGACTGGA TCCAGAAACT CAATAATTTA GGTTTGGATG CTACAAGGAC GTTTTTTTCT 
CGTTATGGTG ATTCTGTTAA AAAGCTTGAT TTTAAATTCA TATCTATGCT CTGGGTTGAA 
AAATATGGTC ATAAACTAGA TCACATAATA TCCAGTTATG AAGAGAGAAA GATAGTTGAA 
TATTCCAATA ATCAAGGATC ATTGAAGGAA GCTCGTTATT TTATATGGCT ATTAGAAGAA 
AATAGAGAAA AGTTTCCAGC ATTGCATAAT GCTTTAGATG AATTCTTTGA TATAATGGAT 
GGTCCTTGCA CAATATTAAA GAAGCAAGAG ACTGAAGATA TTTCATCTAA TGGTATTAAG 
GTAAAAAACA AAACCAGAAA AAAGAAGCAC AAGGAGTCAA AGCCTGTTAT CATACTCTCT 
GGAGTTGGTG GAGTATCACA AAATGATGAG AAAGTTGGTA CAGATGAAAG TACTTTACTT 
CAATCAAATT CTTATGACCC ATTTGTAGTA CCCGAATATC TTCGAGATGA AGTAGAAGAA 
TTTGAAGCTC TATATGAAAA AAATAGTTCA AATAATTGTA ATTATCAGAA GTTATTGGAC 
AGTAACTTAG ATCCAAAATG TGAACCATTA TATGACTATT TTTCTCAGAT CCTGGAAGAA 
CATGGTCCTT TGGAAATTGA TGACAAATTA TTGGTTGGAG AATATGAACA TTTTCCTGAA 
GAAGCTAGGA AAATTGTGGA GGAAGCAGGA GGCTTAAAAT CTTTTTTGTT AGATTCTCTT 
CGTTTTATTA TGATAGACAA TTATATTGGA TTGATGAAAG ATGCTGTTCA ACTTAAGAAA 
AATAAGACCA CTGGAACTGA TGGGGAAGAA AGTACTAGTT TGGATGAGAA AGACTCAATG 
ATTTTCTCTC CACGCTTTCA GAAAAATGCT TCCCAGCTCA AACTACAGTT GAATCCAGCA 
GCTAAAGAAT TTAAACCAGT AACTTGTAAT TCTTTCATAC CAGCACCTAA AAATAAAAAA 
ACAAATAAGA CTGCAAAGTA TGTAACAACA GGATATGTGT CATATATTCC TTTTAATACA 
GTTTTTTCTA ATCAAGGATT TGACACCAGT AACACATCAG TGTCTTATGC AAATATGTTG 
CCACAGTCAT CTCAATGCAT TAGTTTATAT ACAACTGTAT CTGATATATC TTCTGATTAT 
CCAAAAAAAA GAAAACTACC ATTGGTGTCA GCTGTACCTC CTATGACAGA TAATGACAAA 
AGAGATAATT TTGACTCTGG CAATTCCGAG GCATGTAATG TAAAATCTGA AAGACCAATG 
CTTGAAAAAG TATGTTCCTC TGGTAAACCT AATTGTGAAA AAAAGATCCG TCAGAATACT 
CAGAGAACAA CTCTGAAGGC AGAAATATGT AAAAATAATA CTGCCAAAGA TGCATGCCTT 
GGAAGTAATG ATCATTGTGA TCTTGGCACT AGTAAACTAG AACGAGAGAT GAAAAATACG 
ACTATCATCA AAAATACTCC ATGTATACGA ATGGTTGCCA TACAGGTAAA AACTGACTTA 
ATGCATCAGG AGGTTAATAC ACTGCCTTTT GGTCCATTTG AAACCCAACA AGGTGATATT 
TTACGAATGG AAAAGGAACA TCAGGTGTTA CAAGAGCAAC TTAAAGAAGC AAGGGAAAAA 
TATGAACAGT TAAAATGCAG AAGTACAGAG GAAACCAGAG AGGTGGAAGA AAAACTGAAA 
AGAACAATAG AAGAAAATAA GATATCCAAG ACTGAATTGA ATTGGTTCCA TCAAGATTTG 
GAAATAGAAG TGAAAAAATG GCAACAAGAA AAAAAGGAAA ATCAAGAAAA ATTGAAAACT 
TTAAGAAATA AAGTTAAAAA GCTTAATGAT ACTCATGAAA TATATTCAAG AAATAATGAA 
GAAAAGGATC AACAGTATGC ATCACACTTG GATGAATTTC TCAAAATCAG TAATAAATTT 
GCAAATGAAA AATTGAAAAT GGAGGAACTT ATAAAAAAGG GAAAAGAGAA TTATGAGGAA 
ACCATGAAAA GAGCTGTGGC TGCAGAGGTG TCCATACTTG AAAATTGGAA GACTACTGAT 
GTGAGTAAGT TACAGAACAC TGCATCAAAA GCAGAAATGT ATGTGAAGAA TCTAAAGTTA 
ATGAACAGTG ATTCTGCATC ATCTCTTAAT TTGGAATCAG AGATTACTCA CTGGGAATCA 
TTTATTTCAG AAATAAAAAA AGAAATTGAG AAGGCAGAGT TTGAGTTTGA GGATCGAATT 
CAGAAAGTTA AAGAAGGTTC TCAACTGGAT AAACTTTCTA AAGTAAAAGT TCCTGAATTT 
CCATTACCTA CGGATAATTT ATTAATTCAA GACAAGCCTG CTGATATTAA TGATCCAGCC 
TCTGTGACAT ATTCTGCACA AGTTCATTCT GACTTATTTT CTGAATATGA TGATCAAAGT 
CCAGTGATTA TCCCAGCTGA CTTATTAACT GGAAGCCAAG CCCTGACACT TCAGAACACA 
AATCTACATT CTGGCAGTGA CTGCTCAGAG GAGATAACTT CTGTACTTTT ACAAGCATCT 
TATACTGATC CATGTCAGGT CACCCAGCCA TCACCGATTG TTAAGAGTTC TGAAAAGGAT 
ACTCAAATTA ATCAAAGTTC CAAAGGTGTT TCACAGACAC ATGCCAGTAC TCCAAACCAG 
CCACCTTTTA ACAATGTGAT TGAGCATCTG TCAACCATAT TCCCAAATTA CAGCAGTGTT 
GAGCTTTTGA ATTTTATCCA AAAAGTACAA AATCAAGACA GAACTACACA TGCAAGTTTG 
GAGTCTGATG AAATTGTCAG AAGAGTAACA GAACTTATTC TAGATGAGCA GAATAAAAAA 
CTGGCAGTTC AAGGAACAGA CATGAGTGGT GCAGCTGAAT CGTCTGATCT TAGACATTCA 
GCAGAAAATG TATCAGTATC TAGTCCCTCC ATAGTCCCAG CTTCAGAAAC ACTTGCCAGC 
AAACCCTGGG TAGTTGTTGG AGGACCCTCC AAGTCAAAGT GGCAAAAATC ACATGATTCC 
TCTGTCTCCA GTGATGATCC CTGCATAATA TGCTATGAAG AGCTAAACCA GGAACATGTA 
TGTGAGATGG ATTGTGGGCA CCAGTTTCAT AAATGGTGCA TTGAGCAGTG GCTTAAAGAA 
CAAAGTACAT GCCCAACATG CCGTGAATAT GTCCTGTTAG CAGAAGAATT TCCTGCACTT 
TCTGGATCAG GCCGGCCTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_023498851.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023354619.2 Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.

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CloneID	OSa229310
Clone ID Related Accession (Same CDS sequence)	XM_023498851.2
Accession Version	XM_023498851.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase TTC3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_023498851.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGGG AGCATGCCTG GGCACTAGAA GCAAATGAGA GAGCTCAACA TCTTTGCCGA 
AACTATCCAG ATGGAATGAA GGATCTTACT CAACAGAACA TAAAATTACA AAAACAAGTG 
GAAGAATTAA GAGGTTCAAA ACATGGTAGT CATCAGATAA GGAAATCGTT TTATGGAAAA 
AGGTTATCCT CAGGTTATAA TTCTCCAAGA CCTTTACATT ATGGTTTTCT TAATTTTATG 
GAAACTAATG AAGAAAACAA GGTATTGAGA TTAGCATCGA AATATCGTGA TTATTATCAT 
TATCAGAATT TGAAGGTATC TCGTGAAATT ATGAAATTAG AAAATAAAGA CTATCCTCCA 
GATTTGCCAC CAGGTCATAA TCCTAAATTT AAAGGAAAAT CAAAGAATAA AAGCGATGAT 
GTAGGAAAAC CAAGCCTACA ACTGAATTTA CAAATAGATT TACAAAGCTT ATTGGACAAA 
CAATTCAGTA AATCTTCCAG AGCTGTACAA CAGGATTTTG CTATATTGAT AAAAATGCTG 
CAGTCCCTCA TCGAAGAAGG TTGTACAGCT TTGATGGACC AACATTGTCG TAGTGCAGCC 
AAAGCTTTTT CACGGTTGTT AAGTGATTTA GATCCCAATA AATTGAAGCA ACTGAACCTT 
GCCCTTATTA ATTATGTCCT AGTAATCTAT GGTTATGCCA TCTCTCTCCT TGGAATAGGA 
ATGCCTGAGG AATTATCAGA GGCTGAATAT CAATTTAAGA GAATTATTGA GCAATACCCC 
AATGAAGGAG TTGATTGTTT AGCCTATTTT GGAATTGGAA AAGTATATTT GAAAAAAAAT 
AGATTTTCAG ATGCACTTGG TCATTTTCAA AAATCTAAAA CTATGATTAA GCTTATTCCT 
GGAGTGTTGA CTTGGCCTAC AACTAATGTG ATTATTGAAG AGTCTCAAGC TGAGAAATTG 
CAGTTGATAT TGGAAAATCA TATTGAAGAA TGCAAATTTC CACCAGATCC AGATGCAGTT 
TGTTGTTATC AGAAGTGCAA TGGATATTCC AAAATCCAGA TATATTTAAA TGATCCAGAC 
TTTAAGGGTT TTATACGAAT TAACTGCTGC CAGTTTTGTA GAATAGAATT TCATATTAGC 
TGTTGGAAAA AATTAAAAAC AACAATTTTC AATGATAAAA ATGACAAGGA TTTTCTCCAA 
GGAACATGTC TTACTCCTGA CTGTAGAGGT ATTATTTCGA AGATTGTTAT CTTTGGTGCA 
GCTGGCCATA TTAAATGTGA ATTTGAACAT AGGGTCACAA AAGAAAGGTA TCCACCAAGA 
CCTGTTATGA AGCAGAAATG TTCTAGCTTA GAGAAATTGA AAATTAAAGA AGAAAAAAAA 
GTAAAGAGAA AACTTCAAAA AAAAGAAGCA CAAAAAATAG CAAAAGAGAG AATGGAAGAG 
AAAACTAGAG AAAATAATTC ATCTAAGCAT GGCGACCATA AAGGATTTGT ACAAAGTTGC 
CATTTACTTG ATGATAGGAT TTTGCTGTGT ATAAAACAAA ATGCCGAGCA AATTAAATCT 
GGCATCCAGG ACGCTTCCAA ACTTCTCAGA GAATTACTTT CTTGGAAAGT AATAAACACA 
GAGGATTATG CAGATACTTA TTTTAATAGC GGTTTTCTAA GTGAAAAAAT GGAGCATCTC 
ATTGACCATT TGGTACGGAA AAACAATAGA GTAAATACAA GGATTTTTCT GCATGTACTG 
AGTGAGATTG AAGAAATAAA TCCCAAACTA CGTGACTGGA TCCAGAAACT CAATAATTTA 
GGTTTGGATG CTACAAGGAC GTTTTTTTCT CGTTATGGTG ATTCTGTTAA AAAGCTTGAT 
TTTAAATTCA TATCTATGCT CTGGGTTGAA AAATATGGTC ATAAACTAGA TCACATAATA 
TCCAGTTATG AAGAGAGAAA GATAGTTGAA TATTCCAATA ATCAAGGATC ATTGAAGGAA 
GCTCGTTATT TTATATGGCT ATTAGAAGAA AATAGAGAAA AGTTTCCAGC ATTGCATAAT 
GCTTTAGATG AATTCTTTGA TATAATGGAT GGTCCTTGCA CAATATTAAA GAAGCAAGAG 
ACTGAAGATA TTTCATCTAA TGGTATTAAG GTAAAAAACA AAACCAGAAA AAAGAAGCAC 
AAGGAGTCAA AGCCTGTTAT CATACTCTCT GGAGTTGGTG GAGTATCACA AAATGATGAG 
AAAGTTGGTA CAGATGAAAG TACTTTACTT CAATCAAATT CTTATGACCC ATTTGTAGTA 
CCCGAATATC TTCGAGATGA AGTAGAAGAA TTTGAAGCTC TATATGAAAA AAATAGTTCA 
AATAATTGTA ATTATCAGAA GTTATTGGAC AGTAACTTAG ATCCAAAATG TGAACCATTA 
TATGACTATT TTTCTCAGAT CCTGGAAGAA CATGGTCCTT TGGAAATTGA TGACAAATTA 
TTGGTTGGAG AATATGAACA TTTTCCTGAA GAAGCTAGGA AAATTGTGGA GGAAGCAGGA 
GGCTTAAAAT CTTTTTTGTT AGATTCTCTT CGTTTTATTA TGATAGACAA TTATATTGGA 
TTGATGAAAG ATGCTGTTCA ACTTAAGAAA AATAAGACCA CTGGAACTGA TGGGGAAGAA 
AGTACTAGTT TGGATGAGAA AGACTCAATG ATTTTCTCTC CACGCTTTCA GAAAAATGCT 
TCCCAGCTCA AACTACAGTT GAATCCAGCA GCTAAAGAAT TTAAACCAGT AACTTGTAAT 
TCTTTCATAC CAGCACCTAA AAATAAAAAA ACAAATAAGA CTGCAAAGTA TGTAACAACA 
GGATATGTGT CATATATTCC TTTTAATACA GTTTTTTCTA ATCAAGGATT TGACACCAGT 
AACACATCAG TGTCTTATGC AAATATGTTG CCACAGTCAT CTCAATGCAT TAGTTTATAT 
ACAACTGTAT CTGATATATC TTCTGATTAT CCAAAAAAAA GAAAACTACC ATTGGTGTCA 
GCTGTACCTC CTATGACAGA TAATGACAAA AGAGATAATT TTGACTCTGG CAATTCCGAG 
GCATGTAATG TAAAATCTGA AAGACCAATG CTTGAAAAAG TATGTTCCTC TGGTAAACCT 
AATTGTGAAA AAAAGATCCG TCAGAATACT CAGAGAACAA CTCTGAAGGC AGAAATATGT 
AAAAATAATA CTGCCAAAGA TGCATGCCTT GGAAGTAATG ATCATTGTGA TCTTGGCACT 
AGTAAACTAG AACGAGAGAT GAAAAATACG ACTATCATCA AAAATACTCC ATGTATACGA 
ATGGTTGCCA TACAGGTAAA AACTGACTTA ATGCATCAGG AGGTTAATAC ACTGCCTTTT 
GGTCCATTTG AAACCCAACA AGGTGATATT TTACGAATGG AAAAGGAACA TCAGGTGTTA 
CAAGAGCAAC TTAAAGAAGC AAGGGAAAAA TATGAACAGT TAAAATGCAG AAGTACAGAG 
GAAACCAGAG AGGTGGAAGA AAAACTGAAA AGAACAATAG AAGAAAATAA GATATCCAAG 
ACTGAATTGA ATTGGTTCCA TCAAGATTTG GAAATAGAAG TGAAAAAATG GCAACAAGAA 
AAAAAGGAAA ATCAAGAAAA ATTGAAAACT TTAAGAAATA AAGTTAAAAA GCTTAATGAT 
ACTCATGAAA TATATTCAAG AAATAATGAA GAAAAGGATC AACAGTATGC ATCACACTTG 
GATGAATTTC TCAAAATCAG TAATAAATTT GCAAATGAAA AATTGAAAAT GGAGGAACTT 
ATAAAAAAGG GAAAAGAGAA TTATGAGGAA ACCATGAAAA GAGCTGTGGC TGCAGAGGTG 
TCCATACTTG AAAATTGGAA GACTACTGAT GTGAGTAAGT TACAGAACAC TGCATCAAAA 
GCAGAAATGT ATGTGAAGAA TCTAAAGTTA ATGAACAGTG ATTCTGCATC ATCTCTTAAT 
TTGGAATCAG AGATTACTCA CTGGGAATCA TTTATTTCAG AAATAAAAAA AGAAATTGAG 
AAGGCAGAGT TTGAGTTTGA GGATCGAATT CAGAAAGTTA AAGAAGGTTC TCAACTGGAT 
AAACTTTCTA AAGTAAAAGT TCCTGAATTT CCATTACCTA CGGATAATTT ATTAATTCAA 
GACAAGCCTG CTGATATTAA TGATCCAGCC TCTGTGACAT ATTCTGCACA AGTTCATTCT 
GACTTATTTT CTGAATATGA TGATCAAAGT CCAGTGATTA TCCCAGCTGA CTTATTAACT 
GGAAGCCAAG CCCTGACACT TCAGAACACA AATCTACATT CTGGCAGTGA CTGCTCAGAG 
GAGATAACTT CTGTACTTTT ACAAGCATCT TATACTGATC CATGTCAGGT CACCCAGCCA 
TCACCGATTG TTAAGAGTTC TGAAAAGGAT ACTCAAATTA ATCAAAGTTC CAAAGGTGTT 
TCACAGACAC ATGCCAGTAC TCCAAACCAG CCACCTTTTA ACAATGTGAT TGAGCATCTG 
TCAACCATAT TCCCAAATTA CAGCAGTGTT GAGCTTTTGA ATTTTATCCA AAAAGTACAA 
AATCAAGACA GAACTACACA TGCAAGTTTG GAGTCTGATG AAATTGTCAG AAGAGTAACA 
GAACTTATTC TAGATGAGCA GAATAAAAAA CTGGCAGTTC AAGGAACAGA CATGAGTGGT 
GCAGCTGAAT CGTCTGATCT TAGACATTCA GCAGAAAATG TATCAGTATC TAGTCCCTCC 
ATAGTCCCAG CTTCAGAAAC ACTTGCCAGC AAACCCTGGG TAGTTGTTGG AGGACCCTCC 
AAGTCAAAGT GGCAAAAATC ACATGATTCC TCTGTCTCCA GTGATGATCC CTGCATAATA 
TGCTATGAAG AGCTAAACCA GGAACATGTA TGTGAGATGG ATTGTGGGCA CCAGTTTCAT 
AAATGGTGCA TTGAGCAGTG GCTTAAAGAA CAAAGTACAT GCCCAACATG CCGTGAATAT 
GTCCTGTTAG CAGAAGAATT TCCTGCACTT TCTGGATCAG GCCGGCCTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023354619.2
CDS	213..5246
Translation	MLGEHAWALEANERAQHLCRNYPDGMKDLTQQNIKLQKQVEELRGSKHGSHQIRKSFYGKRLSSGYNSPRPLHYGFLNFMETNEENKVLRLASKYRDYYHYQNLKVSREIMKLENKDYPPDLPPGHNPKFKGKSKNKSDDVGKPSLQLNLQIDLQSLLDKQFSKSSRAVQQDFAILIKMLQSLIEEGCTALMDQHCRSAAKAFSRLLSDLDPNKLKQLNLALINYVLVIYGYAISLLGIGMPEELSEAEYQFKRIIEQYPNEGVDCLAYFGIGKVYLKKNRFSDALGHFQKSKTMIKLIPGVLTWPTTNVIIEESQAEKLQLILENHIEECKFPPDPDAVCCYQKCNGYSKIQIYLNDPDFKGFIRINCCQFCRIEFHISCWKKLKTTIFNDKNDKDFLQGTCLTPDCRGIISKIVIFGAAGHIKCEFEHRVTKERYPPRPVMKQKCSSLEKLKIKEEKKVKRKLQKKEAQKIAKERMEEKTRENNSSKHGDHKGFVQSCHLLDDRILLCIKQNAEQIKSGIQDASKLLRELLSWKVINTEDYADTYFNSGFLSEKMEHLIDHLVRKNNRVNTRIFLHVLSEIEEINPKLRDWIQKLNNLGLDATRTFFSRYGDSVKKLDFKFISMLWVEKYGHKLDHIISSYEERKIVEYSNNQGSLKEARYFIWLLEENREKFPALHNALDEFFDIMDGPCTILKKQETEDISSNGIKVKNKTRKKKHKESKPVIILSGVGGVSQNDEKVGTDESTLLQSNSYDPFVVPEYLRDEVEEFEALYEKNSSNNCNYQKLLDSNLDPKCEPLYDYFSQILEEHGPLEIDDKLLVGEYEHFPEEARKIVEEAGGLKSFLLDSLRFIMIDNYIGLMKDAVQLKKNKTTGTDGEESTSLDEKDSMIFSPRFQKNASQLKLQLNPAAKEFKPVTCNSFIPAPKNKKTNKTAKYVTTGYVSYIPFNTVFSNQGFDTSNTSVSYANMLPQSSQCISLYTTVSDISSDYPKKRKLPLVSAVPPMTDNDKRDNFDSGNSEACNVKSERPMLEKVCSSGKPNCEKKIRQNTQRTTLKAEICKNNTAKDACLGSNDHCDLGTSKLEREMKNTTIIKNTPCIRMVAIQVKTDLMHQEVNTLPFGPFETQQGDILRMEKEHQVLQEQLKEAREKYEQLKCRSTEETREVEEKLKRTIEENKISKTELNWFHQDLEIEVKKWQQEKKENQEKLKTLRNKVKKLNDTHEIYSRNNEEKDQQYASHLDEFLKISNKFANEKLKMEELIKKGKENYEETMKRAVAAEVSILENWKTTDVSKLQNTASKAEMYVKNLKLMNSDSASSLNLESEITHWESFISEIKKEIEKAEFEFEDRIQKVKEGSQLDKLSKVKVPEFPLPTDNLLIQDKPADINDPASVTYSAQVHSDLFSEYDDQSPVIIPADLLTGSQALTLQNTNLHSGSDCSEEITSVLLQASYTDPCQVTQPSPIVKSSEKDTQINQSSKGVSQTHASTPNQPPFNNVIEHLSTIFPNYSSVELLNFIQKVQNQDRTTHASLESDEIVRRVTELILDEQNKKLAVQGTDMSGAAESSDLRHSAENVSVSSPSIVPASETLASKPWVVVGGPSKSKWQKSHDSSVSSDDPCIICYEELNQEHVCEMDCGHQFHKWCIEQWLKEQSTCPTCREYVLLAEEFPALSGSGRPA

Target ORF information:

RefSeq Version	XM_023498851.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023498851.2

ORF Insert Sequence:

ATGTTGGGGG AGCATGCCTG GGCACTAGAA GCAAATGAGA GAGCTCAACA TCTTTGCCGA 
AACTATCCAG ATGGAATGAA GGATCTTACT CAACAGAACA TAAAATTACA AAAACAAGTG 
GAAGAATTAA GAGGTTCAAA ACATGGTAGT CATCAGATAA GGAAATCGTT TTATGGAAAA 
AGGTTATCCT CAGGTTATAA TTCTCCAAGA CCTTTACATT ATGGTTTTCT TAATTTTATG 
GAAACTAATG AAGAAAACAA GGTATTGAGA TTAGCATCGA AATATCGTGA TTATTATCAT 
TATCAGAATT TGAAGGTATC TCGTGAAATT ATGAAATTAG AAAATAAAGA CTATCCTCCA 
GATTTGCCAC CAGGTCATAA TCCTAAATTT AAAGGAAAAT CAAAGAATAA AAGCGATGAT 
GTAGGAAAAC CAAGCCTACA ACTGAATTTA CAAATAGATT TACAAAGCTT ATTGGACAAA 
CAATTCAGTA AATCTTCCAG AGCTGTACAA CAGGATTTTG CTATATTGAT AAAAATGCTG 
CAGTCCCTCA TCGAAGAAGG TTGTACAGCT TTGATGGACC AACATTGTCG TAGTGCAGCC 
AAAGCTTTTT CACGGTTGTT AAGTGATTTA GATCCCAATA AATTGAAGCA ACTGAACCTT 
GCCCTTATTA ATTATGTCCT AGTAATCTAT GGTTATGCCA TCTCTCTCCT TGGAATAGGA 
ATGCCTGAGG AATTATCAGA GGCTGAATAT CAATTTAAGA GAATTATTGA GCAATACCCC 
AATGAAGGAG TTGATTGTTT AGCCTATTTT GGAATTGGAA AAGTATATTT GAAAAAAAAT 
AGATTTTCAG ATGCACTTGG TCATTTTCAA AAATCTAAAA CTATGATTAA GCTTATTCCT 
GGAGTGTTGA CTTGGCCTAC AACTAATGTG ATTATTGAAG AGTCTCAAGC TGAGAAATTG 
CAGTTGATAT TGGAAAATCA TATTGAAGAA TGCAAATTTC CACCAGATCC AGATGCAGTT 
TGTTGTTATC AGAAGTGCAA TGGATATTCC AAAATCCAGA TATATTTAAA TGATCCAGAC 
TTTAAGGGTT TTATACGAAT TAACTGCTGC CAGTTTTGTA GAATAGAATT TCATATTAGC 
TGTTGGAAAA AATTAAAAAC AACAATTTTC AATGATAAAA ATGACAAGGA TTTTCTCCAA 
GGAACATGTC TTACTCCTGA CTGTAGAGGT ATTATTTCGA AGATTGTTAT CTTTGGTGCA 
GCTGGCCATA TTAAATGTGA ATTTGAACAT AGGGTCACAA AAGAAAGGTA TCCACCAAGA 
CCTGTTATGA AGCAGAAATG TTCTAGCTTA GAGAAATTGA AAATTAAAGA AGAAAAAAAA 
GTAAAGAGAA AACTTCAAAA AAAAGAAGCA CAAAAAATAG CAAAAGAGAG AATGGAAGAG 
AAAACTAGAG AAAATAATTC ATCTAAGCAT GGCGACCATA AAGGATTTGT ACAAAGTTGC 
CATTTACTTG ATGATAGGAT TTTGCTGTGT ATAAAACAAA ATGCCGAGCA AATTAAATCT 
GGCATCCAGG ACGCTTCCAA ACTTCTCAGA GAATTACTTT CTTGGAAAGT AATAAACACA 
GAGGATTATG CAGATACTTA TTTTAATAGC GGTTTTCTAA GTGAAAAAAT GGAGCATCTC 
ATTGACCATT TGGTACGGAA AAACAATAGA GTAAATACAA GGATTTTTCT GCATGTACTG 
AGTGAGATTG AAGAAATAAA TCCCAAACTA CGTGACTGGA TCCAGAAACT CAATAATTTA 
GGTTTGGATG CTACAAGGAC GTTTTTTTCT CGTTATGGTG ATTCTGTTAA AAAGCTTGAT 
TTTAAATTCA TATCTATGCT CTGGGTTGAA AAATATGGTC ATAAACTAGA TCACATAATA 
TCCAGTTATG AAGAGAGAAA GATAGTTGAA TATTCCAATA ATCAAGGATC ATTGAAGGAA 
GCTCGTTATT TTATATGGCT ATTAGAAGAA AATAGAGAAA AGTTTCCAGC ATTGCATAAT 
GCTTTAGATG AATTCTTTGA TATAATGGAT GGTCCTTGCA CAATATTAAA GAAGCAAGAG 
ACTGAAGATA TTTCATCTAA TGGTATTAAG GTAAAAAACA AAACCAGAAA AAAGAAGCAC 
AAGGAGTCAA AGCCTGTTAT CATACTCTCT GGAGTTGGTG GAGTATCACA AAATGATGAG 
AAAGTTGGTA CAGATGAAAG TACTTTACTT CAATCAAATT CTTATGACCC ATTTGTAGTA 
CCCGAATATC TTCGAGATGA AGTAGAAGAA TTTGAAGCTC TATATGAAAA AAATAGTTCA 
AATAATTGTA ATTATCAGAA GTTATTGGAC AGTAACTTAG ATCCAAAATG TGAACCATTA 
TATGACTATT TTTCTCAGAT CCTGGAAGAA CATGGTCCTT TGGAAATTGA TGACAAATTA 
TTGGTTGGAG AATATGAACA TTTTCCTGAA GAAGCTAGGA AAATTGTGGA GGAAGCAGGA 
GGCTTAAAAT CTTTTTTGTT AGATTCTCTT CGTTTTATTA TGATAGACAA TTATATTGGA 
TTGATGAAAG ATGCTGTTCA ACTTAAGAAA AATAAGACCA CTGGAACTGA TGGGGAAGAA 
AGTACTAGTT TGGATGAGAA AGACTCAATG ATTTTCTCTC CACGCTTTCA GAAAAATGCT 
TCCCAGCTCA AACTACAGTT GAATCCAGCA GCTAAAGAAT TTAAACCAGT AACTTGTAAT 
TCTTTCATAC CAGCACCTAA AAATAAAAAA ACAAATAAGA CTGCAAAGTA TGTAACAACA 
GGATATGTGT CATATATTCC TTTTAATACA GTTTTTTCTA ATCAAGGATT TGACACCAGT 
AACACATCAG TGTCTTATGC AAATATGTTG CCACAGTCAT CTCAATGCAT TAGTTTATAT 
ACAACTGTAT CTGATATATC TTCTGATTAT CCAAAAAAAA GAAAACTACC ATTGGTGTCA 
GCTGTACCTC CTATGACAGA TAATGACAAA AGAGATAATT TTGACTCTGG CAATTCCGAG 
GCATGTAATG TAAAATCTGA AAGACCAATG CTTGAAAAAG TATGTTCCTC TGGTAAACCT 
AATTGTGAAA AAAAGATCCG TCAGAATACT CAGAGAACAA CTCTGAAGGC AGAAATATGT 
AAAAATAATA CTGCCAAAGA TGCATGCCTT GGAAGTAATG ATCATTGTGA TCTTGGCACT 
AGTAAACTAG AACGAGAGAT GAAAAATACG ACTATCATCA AAAATACTCC ATGTATACGA 
ATGGTTGCCA TACAGGTAAA AACTGACTTA ATGCATCAGG AGGTTAATAC ACTGCCTTTT 
GGTCCATTTG AAACCCAACA AGGTGATATT TTACGAATGG AAAAGGAACA TCAGGTGTTA 
CAAGAGCAAC TTAAAGAAGC AAGGGAAAAA TATGAACAGT TAAAATGCAG AAGTACAGAG 
GAAACCAGAG AGGTGGAAGA AAAACTGAAA AGAACAATAG AAGAAAATAA GATATCCAAG 
ACTGAATTGA ATTGGTTCCA TCAAGATTTG GAAATAGAAG TGAAAAAATG GCAACAAGAA 
AAAAAGGAAA ATCAAGAAAA ATTGAAAACT TTAAGAAATA AAGTTAAAAA GCTTAATGAT 
ACTCATGAAA TATATTCAAG AAATAATGAA GAAAAGGATC AACAGTATGC ATCACACTTG 
GATGAATTTC TCAAAATCAG TAATAAATTT GCAAATGAAA AATTGAAAAT GGAGGAACTT 
ATAAAAAAGG GAAAAGAGAA TTATGAGGAA ACCATGAAAA GAGCTGTGGC TGCAGAGGTG 
TCCATACTTG AAAATTGGAA GACTACTGAT GTGAGTAAGT TACAGAACAC TGCATCAAAA 
GCAGAAATGT ATGTGAAGAA TCTAAAGTTA ATGAACAGTG ATTCTGCATC ATCTCTTAAT 
TTGGAATCAG AGATTACTCA CTGGGAATCA TTTATTTCAG AAATAAAAAA AGAAATTGAG 
AAGGCAGAGT TTGAGTTTGA GGATCGAATT CAGAAAGTTA AAGAAGGTTC TCAACTGGAT 
AAACTTTCTA AAGTAAAAGT TCCTGAATTT CCATTACCTA CGGATAATTT ATTAATTCAA 
GACAAGCCTG CTGATATTAA TGATCCAGCC TCTGTGACAT ATTCTGCACA AGTTCATTCT 
GACTTATTTT CTGAATATGA TGATCAAAGT CCAGTGATTA TCCCAGCTGA CTTATTAACT 
GGAAGCCAAG CCCTGACACT TCAGAACACA AATCTACATT CTGGCAGTGA CTGCTCAGAG 
GAGATAACTT CTGTACTTTT ACAAGCATCT TATACTGATC CATGTCAGGT CACCCAGCCA 
TCACCGATTG TTAAGAGTTC TGAAAAGGAT ACTCAAATTA ATCAAAGTTC CAAAGGTGTT 
TCACAGACAC ATGCCAGTAC TCCAAACCAG CCACCTTTTA ACAATGTGAT TGAGCATCTG 
TCAACCATAT TCCCAAATTA CAGCAGTGTT GAGCTTTTGA ATTTTATCCA AAAAGTACAA 
AATCAAGACA GAACTACACA TGCAAGTTTG GAGTCTGATG AAATTGTCAG AAGAGTAACA 
GAACTTATTC TAGATGAGCA GAATAAAAAA CTGGCAGTTC AAGGAACAGA CATGAGTGGT 
GCAGCTGAAT CGTCTGATCT TAGACATTCA GCAGAAAATG TATCAGTATC TAGTCCCTCC 
ATAGTCCCAG CTTCAGAAAC ACTTGCCAGC AAACCCTGGG TAGTTGTTGG AGGACCCTCC 
AAGTCAAAGT GGCAAAAATC ACATGATTCC TCTGTCTCCA GTGATGATCC CTGCATAATA 
TGCTATGAAG AGCTAAACCA GGAACATGTA TGTGAGATGG ATTGTGGGCA CCAGTTTCAT 
AAATGGTGCA TTGAGCAGTG GCTTAAAGAA CAAAGTACAT GCCCAACATG CCGTGAATAT 
GTCCTGTTAG CAGAAGAATT TCCTGCACTT TCTGGATCAG GCCGGCCTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TTC3 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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