LOC100927741 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100927741
Entrez Gene ID	100927741
Full Name	WD repeat-containing protein 7
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031945927.1	XP_031801787.1	WD repeat-containing protein 7 isoform X3
XM_023496175.1	XP_023351943.1	WD repeat-containing protein 7
XM_031945925.1	XP_031801785.1	WD repeat-containing protein 7 isoform X1
XM_031945926.1	XP_031801786.1	WD repeat-containing protein 7 isoform X2
XM_012541485.1	XP_012396939.1	WD repeat-containing protein 7-like

Pathways from BioSystems
Gene Ontology

The following LOC100927741 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100927741 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa121634	XM_012541485.1 Latest version!	Sarcophilus harrisii WD repeat-containing protein 7-like (LOC100927741), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSa220933	XM_031945925.1 Latest version!	Sarcophilus harrisii WD repeat domain 7 (WDR7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa220934	XM_031945926.1 Latest version!	Sarcophilus harrisii WD repeat domain 7 (WDR7), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa220935	XM_031945927.1 Latest version!	Sarcophilus harrisii WD repeat domain 7 (WDR7), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100927741 ( XM_012541485.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012396939.1 Sarcophilus harrisii WD repeat-containing protein 7-like (LOC100927741), mRNA.

Price & Availability↑

CloneID	OSa121634
Clone ID Related Accession (Same CDS sequence)	XM_012541485.1
Accession Version	XM_012541485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	WD repeat-containing protein 7-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823310.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGCAGGAA ATAGCCTTGT TCTACCAATT GTTCTTTGGG GCCGTAAAGC ACCAACACAT 
TGCATCTCAG CTGTGCTTTT AATGGATGAT GGGGCTACAA TTGTAACAGG GTGTCATGAT 
GGACAAATAT GTCTCTGGGA TCTTTCCTTA GAATTGGAAT TGTTTATCTG CCCTCCTGTA 
ACTCGATTCT TTTATGGACG TAGGGAATGC TTCCATAAGC TACTTATTCA GGGAGACTCT 
TCAGGAAGAC TATGTATTTG GAATGTACCA GACACCCTTG AAAAACAGGA AGCTGAAGAA 
GGATTGAAAA TGACAACTTC AATTTGTTTA CAAGAGGCCT TTGGTAAACT CACTCCTTGT 
CCTGCTGGTA TTATAGATCA ACTAAGTTTG GTTCCTAATA GCAGTGACCC TTTGAAAGTT 
ACAGCTAGTG TGTATATCCC ATCCCATGGA CGACTAGTTT GTGGTCGTGA AGATGGAAGC 
ATAATTATTG TGCCAGCAAC ACAAACTGCT ATAGTCCAGC TGTTACAAGG GGAACATATG 
CTCCGGAGAG GTTGGCCACC TCACAGAACT CTTCGGGGTC ATCGGAACAA AATTACATGT 
TTACTATATC CTCATCAGGT CTCAGCTCGT TATGACCAAA GATACTTGAT TTCTGGAGGG 
GTAGATTTTT CTGTCATAAT CTGGGATATA TTTTCTGGAG AAATGAAGCA TATCTTCTGT 
GTGCATGGTG GAGAGATTAC TCAACTCCTT GTTCCACCTG AAAACTGCAG TACAAGAGTG 
CAACACTGCA TCTGCTCTGT TGCCAGTGAC CATTCTGTAG GACTTCTAAG TTTGAGAGAA 
AAGAAATGTA TCATGTTGGC ATCTCGTCAC CTTTTTCCCA TCCAAGTAAT AAAATGGAGG 
CCTTCAGATG ATTATCTAGT GGTTGGCTGT TCAGATGGCT CTGTGTATGT CTGGCAAATG 
GATACTGGTG CACTGGATCG TTGTGTGATG GGGATTACTG CAGTTGAGAT TTTGAATGCT 
TGTGATGAAG CAGTTCCTGC TGCAGTTGAT TCTCTTAGTC ATCCAGCTAT TAATTTAAAA 
CAGGCTATGA CAAGACGTAG TCTTGCTGCT TTAAAAAATA TGGCTCATCA TAAGTTACAG 
ACTCTTGCCA CTAACCTCTT GGCTTCTGAA GCTTCCGACA AAGGAAATTT ACCTAAGTAT 
TCTCATAATT CTCTAATGGT TCAAGCAATA AAGACTAACC TAACAGATCC GGACATACAT 
GTGCTCTTCT TTGATGTGGA AGCTTTGATT ATTCAGCTAC TGACTGAAGA AGCCTCTAGG 
CCGAATACTG CCCTTATTTC CCCAGAGAGT TTGCATAAAG CATCAGGCAG TGCTGACAAA 
GGGGGCTCTT TCCTGACTGG CAAAAGAGCT GCCGTACTCT TCCAGCAAGT CAAAGAAACA 
ATCAAAGAGA ACATAAAGGA GCATCTTCTT GATGATGAAG ATGAGGACGA GGAGATACTG 
AGGCAGAAGA GAGAAGAAAG TGACCCAGAG TATCGATCTA GCAAATCTAA GCCATTGACT 
TTATTAGAGT ATAACCTAAC AATGGATACT GCCAAGTTGT TTATGTCCTG TCTTCATGCC 
TGGGGTTTGA ATGAAGTTCT AGATGAAGTT TGTCTTGACC GCCTTGGAAT GCTTAAACCC 
CACTGTACAG TCTCCTTTGG TCTTCTGTCA AGAGGAGGCC ACATGTCATT GATGCTTCCT 
GGTCATAACC AGCCTATATG TAAGCCATCC TGTGGAAAAC CAGAAGGGGA AAGAAAACTC 
TCAACCACAG ATGGAGTAGG AAAAGGAACC TATGGAGTAT CCCGGGCTGT TACCACTCAG 
CACCTTCTGT CTATTATATC TTTGGCAAAC ACCTTAATGA GTATGACCAA TGCAACTTTT 
ATTGGTGATC ATATGAAGAA GGGCCCTGTC AGGCCACCTA GGCCAAGCAC CCCAGACTTT 
TCCAAAGTGA AGGGTTCCCC TCCAACTTCC AGTAATGTTG TTCAAGGACA GATTAAACAA 
GGATGGAGTC AACTAGCTGC TATGCATTGT GTTATGCTTC CAGACCTGTT GGGATTGGAT 
AAATTTAGAC CTCCACTTCT AGAAATGCTG GCTCGGAGAT GGCAGGATCG ATGCCTGGAG 
GTAAGAGAAG CGGCTCAAGC TTTACTGTTG GCAGAACTGA GAAGAATTGA ACAAGGAGGA 
AGAAAAGAAA CAATTGATGC TTGGGCTCCT TATTTACCTC AGTATATGGA TAGTGTTATA 
TCACAAATGA TTGAAAGTCA CTTCACTGAA GATCCATCAT CTCCCCTAAA AGATGATGTG 
CAGTCTTGCT GGGCAGTTAA TTCTTCATTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012396939.1
CDS	1..2373
Translation	MAGNSLVLPIVLWGRKAPTHCISAVLLMDDGATIVTGCHDGQICLWDLSLELELFICPPVTRFFYGRRECFHKLLIQGDSSGRLCIWNVPDTLEKQEAEEGLKMTTSICLQEAFGKLTPCPAGIIDQLSLVPNSSDPLKVTASVYIPSHGRLVCGREDGSIIIVPATQTAIVQLLQGEHMLRRGWPPHRTLRGHRNKITCLLYPHQVSARYDQRYLISGGVDFSVIIWDIFSGEMKHIFCVHGGEITQLLVPPENCSTRVQHCICSVASDHSVGLLSLREKKCIMLASRHLFPIQVIKWRPSDDYLVVGCSDGSVYVWQMDTGALDRCVMGITAVEILNACDEAVPAAVDSLSHPAINLKQAMTRRSLAALKNMAHHKLQTLATNLLASEASDKGNLPKYSHNSLMVQAIKTNLTDPDIHVLFFDVEALIIQLLTEEASRPNTALISPESLHKASGSADKGGSFLTGKRAAVLFQQVKETIKENIKEHLLDDEDEDEEILRQKREESDPEYRSSKSKPLTLLEYNLTMDTAKLFMSCLHAWGLNEVLDEVCLDRLGMLKPHCTVSFGLLSRGGHMSLMLPGHNQPICKPSCGKPEGERKLSTTDGVGKGTYGVSRAVTTQHLLSIISLANTLMSMTNATFIGDHMKKGPVRPPRPSTPDFSKVKGSPPTSSNVVQGQIKQGWSQLAAMHCVMLPDLLGLDKFRPPLLEMLARRWQDRCLEVREAAQALLLAELRRIEQGGRKETIDAWAPYLPQYMDSVISQMIESHFTEDPSSPLKDDVQSCWAVNSSL

Target ORF information:

RefSeq Version	XM_012541485.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WD repeat-containing protein 7-like (LOC100927741), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012541485.1

ORF Insert Sequence:

ATGGCAGGAA ATAGCCTTGT TCTACCAATT GTTCTTTGGG GCCGTAAAGC ACCAACACAT 
TGCATCTCAG CTGTGCTTTT AATGGATGAT GGGGCTACAA TTGTAACAGG GTGTCATGAT 
GGACAAATAT GTCTCTGGGA TCTTTCCTTA GAATTGGAAT TGTTTATCTG CCCTCCTGTA 
ACTCGATTCT TTTATGGACG TAGGGAATGC TTCCATAAGC TACTTATTCA GGGAGACTCT 
TCAGGAAGAC TATGTATTTG GAATGTACCA GACACCCTTG AAAAACAGGA AGCTGAAGAA 
GGATTGAAAA TGACAACTTC AATTTGTTTA CAAGAGGCCT TTGGTAAACT CACTCCTTGT 
CCTGCTGGTA TTATAGATCA ACTAAGTTTG GTTCCTAATA GCAGTGACCC TTTGAAAGTT 
ACAGCTAGTG TGTATATCCC ATCCCATGGA CGACTAGTTT GTGGTCGTGA AGATGGAAGC 
ATAATTATTG TGCCAGCAAC ACAAACTGCT ATAGTCCAGC TGTTACAAGG GGAACATATG 
CTCCGGAGAG GTTGGCCACC TCACAGAACT CTTCGGGGTC ATCGGAACAA AATTACATGT 
TTACTATATC CTCATCAGGT CTCAGCTCGT TATGACCAAA GATACTTGAT TTCTGGAGGG 
GTAGATTTTT CTGTCATAAT CTGGGATATA TTTTCTGGAG AAATGAAGCA TATCTTCTGT 
GTGCATGGTG GAGAGATTAC TCAACTCCTT GTTCCACCTG AAAACTGCAG TACAAGAGTG 
CAACACTGCA TCTGCTCTGT TGCCAGTGAC CATTCTGTAG GACTTCTAAG TTTGAGAGAA 
AAGAAATGTA TCATGTTGGC ATCTCGTCAC CTTTTTCCCA TCCAAGTAAT AAAATGGAGG 
CCTTCAGATG ATTATCTAGT GGTTGGCTGT TCAGATGGCT CTGTGTATGT CTGGCAAATG 
GATACTGGTG CACTGGATCG TTGTGTGATG GGGATTACTG CAGTTGAGAT TTTGAATGCT 
TGTGATGAAG CAGTTCCTGC TGCAGTTGAT TCTCTTAGTC ATCCAGCTAT TAATTTAAAA 
CAGGCTATGA CAAGACGTAG TCTTGCTGCT TTAAAAAATA TGGCTCATCA TAAGTTACAG 
ACTCTTGCCA CTAACCTCTT GGCTTCTGAA GCTTCCGACA AAGGAAATTT ACCTAAGTAT 
TCTCATAATT CTCTAATGGT TCAAGCAATA AAGACTAACC TAACAGATCC GGACATACAT 
GTGCTCTTCT TTGATGTGGA AGCTTTGATT ATTCAGCTAC TGACTGAAGA AGCCTCTAGG 
CCGAATACTG CCCTTATTTC CCCAGAGAGT TTGCATAAAG CATCAGGCAG TGCTGACAAA 
GGGGGCTCTT TCCTGACTGG CAAAAGAGCT GCCGTACTCT TCCAGCAAGT CAAAGAAACA 
ATCAAAGAGA ACATAAAGGA GCATCTTCTT GATGATGAAG ATGAGGACGA GGAGATACTG 
AGGCAGAAGA GAGAAGAAAG TGACCCAGAG TATCGATCTA GCAAATCTAA GCCATTGACT 
TTATTAGAGT ATAACCTAAC AATGGATACT GCCAAGTTGT TTATGTCCTG TCTTCATGCC 
TGGGGTTTGA ATGAAGTTCT AGATGAAGTT TGTCTTGACC GCCTTGGAAT GCTTAAACCC 
CACTGTACAG TCTCCTTTGG TCTTCTGTCA AGAGGAGGCC ACATGTCATT GATGCTTCCT 
GGTCATAACC AGCCTATATG TAAGCCATCC TGTGGAAAAC CAGAAGGGGA AAGAAAACTC 
TCAACCACAG ATGGAGTAGG AAAAGGAACC TATGGAGTAT CCCGGGCTGT TACCACTCAG 
CACCTTCTGT CTATTATATC TTTGGCAAAC ACCTTAATGA GTATGACCAA TGCAACTTTT 
ATTGGTGATC ATATGAAGAA GGGCCCTGTC AGGCCACCTA GGCCAAGCAC CCCAGACTTT 
TCCAAAGTGA AGGGTTCCCC TCCAACTTCC AGTAATGTTG TTCAAGGACA GATTAAACAA 
GGATGGAGTC AACTAGCTGC TATGCATTGT GTTATGCTTC CAGACCTGTT GGGATTGGAT 
AAATTTAGAC CTCCACTTCT AGAAATGCTG GCTCGGAGAT GGCAGGATCG ATGCCTGGAG 
GTAAGAGAAG CGGCTCAAGC TTTACTGTTG GCAGAACTGA GAAGAATTGA ACAAGGAGGA 
AGAAAAGAAA CAATTGATGC TTGGGCTCCT TATTTACCTC AGTATATGGA TAGTGTTATA 
TCACAAATGA TTGAAAGTCA CTTCACTGAA GATCCATCAT CTCCCCTAAA AGATGATGTG 
CAGTCTTGCT GGGCAGTTAA TTCTTCATTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100927741 ( XM_031945925.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031801785.1 Sarcophilus harrisii WD repeat domain 7 (WDR7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa220933
Clone ID Related Accession (Same CDS sequence)	XM_031945925.1
Accession Version	XM_031945925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	WD repeat-containing protein 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA ATAGCCTTGT TCTACCAATT GTTCTTTGGG GCCGTAAAGC ACCAACACAT 
TGCATCTCAG CTGTGCTTTT AATGGATGAT GGGGCTACAA TTGTAACAGG GTGTCATGAT 
GGACAAATAT GTCTCTGGGA TCTTTCCTTA GAATTGGAAA TCAATCCTCG GGCTCTCTTG 
TTTGGCCATA CAGCTTCAAT TACTTGTTTG TCGAAAGCCT GTGCTTCTAG TGACCGTCAG 
TATGTAGTGA GTGCATCTGA AAGTGGAGAA ATGTGCCTTT GGGATGTAAG TGATGGCAGA 
TGTATTGAGT TTACAAAATT AGCTTGCACA CATACTGGCA TAGAGTTCTA CCAGTTCACT 
GTTGGGAATC AGCGTGAAGG GAGACTGCTG TGCCATGGAC ATTACCCTGA AATTCTGGTT 
GTGGATGCCA CCAGCCTTGA GGTTCTTTAT TCTTTAGTGT CAAAGATATC TCCTGACTGG 
ATTAGCTCCA TGAGTATTAT TCGATCTCAC CGAACACAAG AGGATACTGT TGTAGCAGTT 
TCAATGACAG GAATTTTGAA GGTTTGGATT GTTACATCTG AAATTAGTGA TACCCAGGAT 
ACGGAGCCAA TATTTGAAGA AGAATCCAAA CCAATTTATT GTCAGAATTG CCAAAGTATC 
TCTTTCTGTG CATTCACCCA AAGGTCACTT TTGGTTGTGT GTTCCAAATA TTGGAGGGTA 
TTTGATGCTG GAGATTATTC TCTATTATGC TCTGGCCCCA GTGAAAATGG ACAAACATGG 
ACAGGTGGTG ACTTCATATC AGCAGATAAA GTAATTATAT GGACAGAAAA TGGACAGAGC 
TATATTTACA AACTACCTGC CAGTTGCCTG CCTGCTAGTG ATTCATTTCG CAGTGATGTT 
GGAAAGGCTG TTGAAAATTT AATACCTCCT GTACAGTATG GTCTCTTGGA TAGAGAAGAT 
AAAGAGTTGT TTATCTGCCC TCCTGTAACT CGATTCTTTT ATGGACGTAG GGAATGCTTC 
CATAAGCTAC TTATTCAGGG AGACTCTTCA GGAAGACTAT GTATTTGGAA TGTACCAGAC 
ACCCTTGAAA AACAGGAAGC TGAAGAAGGA TTGAAAATGA CAACTTCAAT TTGTTTACAA 
GAGGCCTTTG GTAAACTCAC TCCTTGTCCT GCTGGTATTA TAGATCAACT AAGTTTGGTT 
CCTAATAGCA GTGACCCTTT GAAAGTTACA GCTAGTGTGT ATATCCCATC CCATGGACGA 
CTAGTTTGTG GTCGTGAAGA TGGAAGCATA ATTATTGTGC CAGCAACACA AACTGCTATA 
GTCCAGCTGT TACAAGGGGA ACATATGCTC CGGAGAGGTT GGCCACCTCA CAGAACTCTT 
CGGGGTCATC GGAACAAAAT TACATGTTTA CTATATCCTC ATCAGGTCTC AGCTCGTTAT 
GACCAAAGAT ACTTGATTTC TGGAGGGGTA GATTTTTCTG TCATAATCTG GGATATATTT 
TCTGGAGAAA TGAAGCATAT CTTCTGTGTG CATGGTGGAG AGATTACTCA ACTCCTTGTT 
CCACCTGAAA ACTGCAGTAC AAGAGTGCAA CACTGCATCT GCTCTGTTGC CAGTGACCAT 
TCTGTAGGAC TTCTAAGTTT GAGAGAAAAG AAATGTATCA TGTTGGCATC TCGTCACCTT 
TTTCCCATCC AAGTAATAAA ATGGAGGCCT TCAGATGATT ATCTAGTGGT TGGCTGTTCA 
GATGGCTCTG TGTATGTCTG GCAAATGGAT ACTGGTGCAC TGGATCGTTG TGTGATGGGG 
ATTACTGCAG TTGAGATTTT GAATGCTTGT GATGAAGCAG TTCCTGCTGC AGTTGATTCT 
CTTAGTCATC CAGCTATTAA TTTAAAACAG GCTATGACAA GACGTAGTCT TGCTGCTTTA 
AAAAATATGG CTCATCATAA GTTACAGACT CTTGCCACTA ACCTCTTGGC TTCTGAAGCT 
TCCGACAAAG GAAATTTACC TAAGTATTCT CATAATTCTC TAATGGTTCA AGCAATAAAG 
ACTAACCTAA CAGATCCGGA CATACATGTG CTCTTCTTTG ATGTGGAAGC TTTGATTATT 
CAGCTACTGA CTGAAGAAGC CTCTAGGCCG AATACTGCCC TTATTTCCCC AGAGAGTTTG 
CATAAAGCAT CAGGCAGTGC TGACAAAGGG GGCTCTTTCC TGACTGGCAA AAGAGCTGCC 
GTACTCTTCC AGCAAGTCAA AGAAACAATC AAAGAGAACA TAAAGGAGCA TCTTCTTGAT 
GATGAAGATG AGGACGAGGA GATACTGAGG CAGAAGAGAG AAGAAAGTGA CCCAGAGTAT 
CGATCTAGCA AATCTAAGCC ATTGACTTTA TTAGAGTATA ACCTAACAAT GGATACTGCC 
AAGTTGTTTA TGTCCTGTCT TCATGCCTGG GGTTTGAATG AAGTTCTAGA TGAAGTTTGT 
CTTGACCGCC TTGGAATGCT TAAACCCCAC TGTACAGTCT CCTTTGGTCT TCTGTCAAGA 
GGAGGCCACA TGTCATTGAT GCTTCCTGGT CATAACCAGC CTATATGTAA GCCATCCTGT 
GGAAAACCAG AAGGGGAAAG AAAACTCTCA ACCACAGATG GAGTAGGAAA AGGAACCTAT 
GGAGTATCCC GGGCTGTTAC CACTCAGCAC CTTCTGTCTA TTATATCTTT GGCAAACACC 
TTAATGAGTA TGACCAATGC AACTTTTATT GGTGATCATA TGAAGAAGGG CCCTGTCAGG 
CCACCTAGGC CAAGCACCCC AGACTTTTCC AAAGTGAAGG GTTCCCCTCC AACTTCCAGT 
AATGTTGTTC AAGGACAGAT TAAACAAGCT GCTGCACCTG TCGTTTCCGC TCGGTCTGAT 
ACTGATCACT CTGGTTCTGA CCCTGCTCCT ACTCCTGCTT TACATGCCTG TTTCTTAGTA 
AATGAAGGAT GGAGTCAACT AGCTGCTATG CATTGTGTTA TGCTTCCAGA CCTGTTGGGA 
TTGGATAAAT TTAGACCTCC ACTTCTAGAA ATGCTGGCTC GGAGATGGCA GGATCGATGC 
CTGGAGGTAA GAGAAGCGGC TCAAGCTTTA CTGTTGGCAG AACTGAGAAG AATTGAACAA 
GGAGGAAGAA AAGAAACAAT TGATGCTTGG GCTCCTTATT TACCTCAGTA TATGGATAGT 
GTTATATCAC CTGGAGTGAC AGCAGAATCC ATTCAGACTG CAAATGCTAG TCCAGATGCC 
TTGGGGACTG AAACTAAAGT CCAAGAAGAA GAGCATGATC TTGTTGATGA TGACATCACA 
GCAGGGTGTC TATCATCAGG TCTCCCACAC ATGAAGAAAA TATCCACATC ATATGAAGAA 
AGACGTAAAC AAGCTACAGC TATTGTTTTA TTAGGAGTTA TAGGAGCCGA ATTTGGTGCA 
GAAATTGAAC CTCCGAAATT GTTGACAAGA CCTCGAAGTT CCAGCCAAAT TCCCGAAGGG 
TTTGGTTTGA CCAGTGGTGG ATCCAACTAT TCATTAGCCA GACATACTTG CAAGGCATTG 
ACGTTTCTTC TCCTACAACC TCCTAGTCCT AAACTACCAC CTCACAGCAC AATCAGAAGA 
ACTGCTATTG ATCTAATAGG ACGAGGTTTT ACAGTATGGG AGCCTTATAT GGATGTGTCT 
GCTGTTCTAA TGGGCCTTCT CGAACTCTGT GCTGATGCTG AGAAGCAACT GGCTAACATC 
ACAATGGGGC TGCCCCTGAG TCCAGCAGCT GACTCCGCCC GCTCCGCAAG ACATGCCCTC 
TCACTCATTG CCACTGCCAG ACCACCCGCC TTCATCACAA CTATTGCCAA GGAGGTACAT 
CGACATACTG CTCTAGCAGC AAACACCCAG TCACAGCAGA ATATTCATAC TACAACTCTT 
GCTCGAGCTA AAGGGGAGAT TCTGAGAGTC ATTGAAATTC TTATTGAAAA AATGCCCACA 
GATGTGGTTG ATCTACTTGT TGAGGTTATG GACATTATAA TGTATTGTCT TGAAGGATCT 
TTAGTTAAAA AGAAGGGACT TCAAGAATGT TTCCCAGCCA TCTGCAGGTT CTACATGGTC 
AGCTATTATG AACGAAATCA CAGAATAGCA GTTGGAGCTC GCCATGGTTC AGTGGCCCTG 
TACGACATCC GGACTGGAAA ATGTCAGACA ATTCATGGGC ACAAGGGGCC AATAACTGCT 
GTGGCATTTG CTCCTGATGG AAGGTATCTT GCCACCTACT CAAATTCTGA CAGTCACATT 
TCCTTCTGGC AGATGAACAC CTCACTTTTA GGAAGTATTG GTATGCTGAA CTCAGCACCT 
CAGCTTCGTT GCATTAAAAC TTACCAAGTG CCCCCTGTCC AACCTGCATC CCCTGGATCA 
CATAATGCAC TCAGACTCGC CCGGCTGATC TGGACTTCAA ATCGCAATGT TATTCTTATG 
GCCCATGATG GAAAGGAGCA TCGATTTATG GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031801785.1
CDS	285..4760
Translation	MAGNSLVLPIVLWGRKAPTHCISAVLLMDDGATIVTGCHDGQICLWDLSLELEINPRALLFGHTASITCLSKACASSDRQYVVSASESGEMCLWDVSDGRCIEFTKLACTHTGIEFYQFTVGNQREGRLLCHGHYPEILVVDATSLEVLYSLVSKISPDWISSMSIIRSHRTQEDTVVAVSMTGILKVWIVTSEISDTQDTEPIFEEESKPIYCQNCQSISFCAFTQRSLLVVCSKYWRVFDAGDYSLLCSGPSENGQTWTGGDFISADKVIIWTENGQSYIYKLPASCLPASDSFRSDVGKAVENLIPPVQYGLLDREDKELFICPPVTRFFYGRRECFHKLLIQGDSSGRLCIWNVPDTLEKQEAEEGLKMTTSICLQEAFGKLTPCPAGIIDQLSLVPNSSDPLKVTASVYIPSHGRLVCGREDGSIIIVPATQTAIVQLLQGEHMLRRGWPPHRTLRGHRNKITCLLYPHQVSARYDQRYLISGGVDFSVIIWDIFSGEMKHIFCVHGGEITQLLVPPENCSTRVQHCICSVASDHSVGLLSLREKKCIMLASRHLFPIQVIKWRPSDDYLVVGCSDGSVYVWQMDTGALDRCVMGITAVEILNACDEAVPAAVDSLSHPAINLKQAMTRRSLAALKNMAHHKLQTLATNLLASEASDKGNLPKYSHNSLMVQAIKTNLTDPDIHVLFFDVEALIIQLLTEEASRPNTALISPESLHKASGSADKGGSFLTGKRAAVLFQQVKETIKENIKEHLLDDEDEDEEILRQKREESDPEYRSSKSKPLTLLEYNLTMDTAKLFMSCLHAWGLNEVLDEVCLDRLGMLKPHCTVSFGLLSRGGHMSLMLPGHNQPICKPSCGKPEGERKLSTTDGVGKGTYGVSRAVTTQHLLSIISLANTLMSMTNATFIGDHMKKGPVRPPRPSTPDFSKVKGSPPTSSNVVQGQIKQAAAPVVSARSDTDHSGSDPAPTPALHACFLVNEGWSQLAAMHCVMLPDLLGLDKFRPPLLEMLARRWQDRCLEVREAAQALLLAELRRIEQGGRKETIDAWAPYLPQYMDSVISPGVTAESIQTANASPDALGTETKVQEEEHDLVDDDITAGCLSSGLPHMKKISTSYEERRKQATAIVLLGVIGAEFGAEIEPPKLLTRPRSSSQIPEGFGLTSGGSNYSLARHTCKALTFLLLQPPSPKLPPHSTIRRTAIDLIGRGFTVWEPYMDVSAVLMGLLELCADAEKQLANITMGLPLSPAADSARSARHALSLIATARPPAFITTIAKEVHRHTALAANTQSQQNIHTTTLARAKGEILRVIEILIEKMPTDVVDLLVEVMDIIMYCLEGSLVKKKGLQECFPAICRFYMVSYYERNHRIAVGARHGSVALYDIRTGKCQTIHGHKGPITAVAFAPDGRYLATYSNSDSHISFWQMNTSLLGSIGMLNSAPQLRCIKTYQVPPVQPASPGSHNALRLARLIWTSNRNVILMAHDGKEHRFMV

Target ORF information:

RefSeq Version	XM_031945925.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WD repeat domain 7 (WDR7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031945925.1

ORF Insert Sequence:

ATGGCAGGAA ATAGCCTTGT TCTACCAATT GTTCTTTGGG GCCGTAAAGC ACCAACACAT 
TGCATCTCAG CTGTGCTTTT AATGGATGAT GGGGCTACAA TTGTAACAGG GTGTCATGAT 
GGACAAATAT GTCTCTGGGA TCTTTCCTTA GAATTGGAAA TCAATCCTCG GGCTCTCTTG 
TTTGGCCATA CAGCTTCAAT TACTTGTTTG TCGAAAGCCT GTGCTTCTAG TGACCGTCAG 
TATGTAGTGA GTGCATCTGA AAGTGGAGAA ATGTGCCTTT GGGATGTAAG TGATGGCAGA 
TGTATTGAGT TTACAAAATT AGCTTGCACA CATACTGGCA TAGAGTTCTA CCAGTTCACT 
GTTGGGAATC AGCGTGAAGG GAGACTGCTG TGCCATGGAC ATTACCCTGA AATTCTGGTT 
GTGGATGCCA CCAGCCTTGA GGTTCTTTAT TCTTTAGTGT CAAAGATATC TCCTGACTGG 
ATTAGCTCCA TGAGTATTAT TCGATCTCAC CGAACACAAG AGGATACTGT TGTAGCAGTT 
TCAATGACAG GAATTTTGAA GGTTTGGATT GTTACATCTG AAATTAGTGA TACCCAGGAT 
ACGGAGCCAA TATTTGAAGA AGAATCCAAA CCAATTTATT GTCAGAATTG CCAAAGTATC 
TCTTTCTGTG CATTCACCCA AAGGTCACTT TTGGTTGTGT GTTCCAAATA TTGGAGGGTA 
TTTGATGCTG GAGATTATTC TCTATTATGC TCTGGCCCCA GTGAAAATGG ACAAACATGG 
ACAGGTGGTG ACTTCATATC AGCAGATAAA GTAATTATAT GGACAGAAAA TGGACAGAGC 
TATATTTACA AACTACCTGC CAGTTGCCTG CCTGCTAGTG ATTCATTTCG CAGTGATGTT 
GGAAAGGCTG TTGAAAATTT AATACCTCCT GTACAGTATG GTCTCTTGGA TAGAGAAGAT 
AAAGAGTTGT TTATCTGCCC TCCTGTAACT CGATTCTTTT ATGGACGTAG GGAATGCTTC 
CATAAGCTAC TTATTCAGGG AGACTCTTCA GGAAGACTAT GTATTTGGAA TGTACCAGAC 
ACCCTTGAAA AACAGGAAGC TGAAGAAGGA TTGAAAATGA CAACTTCAAT TTGTTTACAA 
GAGGCCTTTG GTAAACTCAC TCCTTGTCCT GCTGGTATTA TAGATCAACT AAGTTTGGTT 
CCTAATAGCA GTGACCCTTT GAAAGTTACA GCTAGTGTGT ATATCCCATC CCATGGACGA 
CTAGTTTGTG GTCGTGAAGA TGGAAGCATA ATTATTGTGC CAGCAACACA AACTGCTATA 
GTCCAGCTGT TACAAGGGGA ACATATGCTC CGGAGAGGTT GGCCACCTCA CAGAACTCTT 
CGGGGTCATC GGAACAAAAT TACATGTTTA CTATATCCTC ATCAGGTCTC AGCTCGTTAT 
GACCAAAGAT ACTTGATTTC TGGAGGGGTA GATTTTTCTG TCATAATCTG GGATATATTT 
TCTGGAGAAA TGAAGCATAT CTTCTGTGTG CATGGTGGAG AGATTACTCA ACTCCTTGTT 
CCACCTGAAA ACTGCAGTAC AAGAGTGCAA CACTGCATCT GCTCTGTTGC CAGTGACCAT 
TCTGTAGGAC TTCTAAGTTT GAGAGAAAAG AAATGTATCA TGTTGGCATC TCGTCACCTT 
TTTCCCATCC AAGTAATAAA ATGGAGGCCT TCAGATGATT ATCTAGTGGT TGGCTGTTCA 
GATGGCTCTG TGTATGTCTG GCAAATGGAT ACTGGTGCAC TGGATCGTTG TGTGATGGGG 
ATTACTGCAG TTGAGATTTT GAATGCTTGT GATGAAGCAG TTCCTGCTGC AGTTGATTCT 
CTTAGTCATC CAGCTATTAA TTTAAAACAG GCTATGACAA GACGTAGTCT TGCTGCTTTA 
AAAAATATGG CTCATCATAA GTTACAGACT CTTGCCACTA ACCTCTTGGC TTCTGAAGCT 
TCCGACAAAG GAAATTTACC TAAGTATTCT CATAATTCTC TAATGGTTCA AGCAATAAAG 
ACTAACCTAA CAGATCCGGA CATACATGTG CTCTTCTTTG ATGTGGAAGC TTTGATTATT 
CAGCTACTGA CTGAAGAAGC CTCTAGGCCG AATACTGCCC TTATTTCCCC AGAGAGTTTG 
CATAAAGCAT CAGGCAGTGC TGACAAAGGG GGCTCTTTCC TGACTGGCAA AAGAGCTGCC 
GTACTCTTCC AGCAAGTCAA AGAAACAATC AAAGAGAACA TAAAGGAGCA TCTTCTTGAT 
GATGAAGATG AGGACGAGGA GATACTGAGG CAGAAGAGAG AAGAAAGTGA CCCAGAGTAT 
CGATCTAGCA AATCTAAGCC ATTGACTTTA TTAGAGTATA ACCTAACAAT GGATACTGCC 
AAGTTGTTTA TGTCCTGTCT TCATGCCTGG GGTTTGAATG AAGTTCTAGA TGAAGTTTGT 
CTTGACCGCC TTGGAATGCT TAAACCCCAC TGTACAGTCT CCTTTGGTCT TCTGTCAAGA 
GGAGGCCACA TGTCATTGAT GCTTCCTGGT CATAACCAGC CTATATGTAA GCCATCCTGT 
GGAAAACCAG AAGGGGAAAG AAAACTCTCA ACCACAGATG GAGTAGGAAA AGGAACCTAT 
GGAGTATCCC GGGCTGTTAC CACTCAGCAC CTTCTGTCTA TTATATCTTT GGCAAACACC 
TTAATGAGTA TGACCAATGC AACTTTTATT GGTGATCATA TGAAGAAGGG CCCTGTCAGG 
CCACCTAGGC CAAGCACCCC AGACTTTTCC AAAGTGAAGG GTTCCCCTCC AACTTCCAGT 
AATGTTGTTC AAGGACAGAT TAAACAAGCT GCTGCACCTG TCGTTTCCGC TCGGTCTGAT 
ACTGATCACT CTGGTTCTGA CCCTGCTCCT ACTCCTGCTT TACATGCCTG TTTCTTAGTA 
AATGAAGGAT GGAGTCAACT AGCTGCTATG CATTGTGTTA TGCTTCCAGA CCTGTTGGGA 
TTGGATAAAT TTAGACCTCC ACTTCTAGAA ATGCTGGCTC GGAGATGGCA GGATCGATGC 
CTGGAGGTAA GAGAAGCGGC TCAAGCTTTA CTGTTGGCAG AACTGAGAAG AATTGAACAA 
GGAGGAAGAA AAGAAACAAT TGATGCTTGG GCTCCTTATT TACCTCAGTA TATGGATAGT 
GTTATATCAC CTGGAGTGAC AGCAGAATCC ATTCAGACTG CAAATGCTAG TCCAGATGCC 
TTGGGGACTG AAACTAAAGT CCAAGAAGAA GAGCATGATC TTGTTGATGA TGACATCACA 
GCAGGGTGTC TATCATCAGG TCTCCCACAC ATGAAGAAAA TATCCACATC ATATGAAGAA 
AGACGTAAAC AAGCTACAGC TATTGTTTTA TTAGGAGTTA TAGGAGCCGA ATTTGGTGCA 
GAAATTGAAC CTCCGAAATT GTTGACAAGA CCTCGAAGTT CCAGCCAAAT TCCCGAAGGG 
TTTGGTTTGA CCAGTGGTGG ATCCAACTAT TCATTAGCCA GACATACTTG CAAGGCATTG 
ACGTTTCTTC TCCTACAACC TCCTAGTCCT AAACTACCAC CTCACAGCAC AATCAGAAGA 
ACTGCTATTG ATCTAATAGG ACGAGGTTTT ACAGTATGGG AGCCTTATAT GGATGTGTCT 
GCTGTTCTAA TGGGCCTTCT CGAACTCTGT GCTGATGCTG AGAAGCAACT GGCTAACATC 
ACAATGGGGC TGCCCCTGAG TCCAGCAGCT GACTCCGCCC GCTCCGCAAG ACATGCCCTC 
TCACTCATTG CCACTGCCAG ACCACCCGCC TTCATCACAA CTATTGCCAA GGAGGTACAT 
CGACATACTG CTCTAGCAGC AAACACCCAG TCACAGCAGA ATATTCATAC TACAACTCTT 
GCTCGAGCTA AAGGGGAGAT TCTGAGAGTC ATTGAAATTC TTATTGAAAA AATGCCCACA 
GATGTGGTTG ATCTACTTGT TGAGGTTATG GACATTATAA TGTATTGTCT TGAAGGATCT 
TTAGTTAAAA AGAAGGGACT TCAAGAATGT TTCCCAGCCA TCTGCAGGTT CTACATGGTC 
AGCTATTATG AACGAAATCA CAGAATAGCA GTTGGAGCTC GCCATGGTTC AGTGGCCCTG 
TACGACATCC GGACTGGAAA ATGTCAGACA ATTCATGGGC ACAAGGGGCC AATAACTGCT 
GTGGCATTTG CTCCTGATGG AAGGTATCTT GCCACCTACT CAAATTCTGA CAGTCACATT 
TCCTTCTGGC AGATGAACAC CTCACTTTTA GGAAGTATTG GTATGCTGAA CTCAGCACCT 
CAGCTTCGTT GCATTAAAAC TTACCAAGTG CCCCCTGTCC AACCTGCATC CCCTGGATCA 
CATAATGCAC TCAGACTCGC CCGGCTGATC TGGACTTCAA ATCGCAATGT TATTCTTATG 
GCCCATGATG GAAAGGAGCA TCGATTTATG GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100927741 ( XM_031945926.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031801786.1 Sarcophilus harrisii WD repeat domain 7 (WDR7), transcript variant X4, mRNA.

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CloneID	OSa220934
Clone ID Related Accession (Same CDS sequence)	XM_031945926.1
Accession Version	XM_031945926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	WD repeat-containing protein 7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA ATAGCCTTGT TCTACCAATT GTTCTTTGGG GCCGTAAAGC ACCAACACAT 
TGCATCTCAG CTGTGCTTTT AATGGATGAT GGGGCTACAA TTGTAACAGG GTGTCATGAT 
GGACAAATAT GTCTCTGGGA TCTTTCCTTA GAATTGGAAA TCAATCCTCG GGCTCTCTTG 
TTTGGCCATA CAGCTTCAAT TACTTGTTTG TCGAAAGCCT GTGCTTCTAG TGACCGTCAG 
TATGTAGTGA GTGCATCTGA AAGTGGAGAA ATGTGCCTTT GGGATGTAAG TGATGGCAGA 
TGTATTGAGT TTACAAAATT AGCTTGCACA CATACTGGCA TAGAGTTCTA CCAGTTCACT 
GTTGGGAATC AGCGTGAAGG GAGACTGCTG TGCCATGGAC ATTACCCTGA AATTCTGGTT 
GTGGATGCCA CCAGCCTTGA GGTTCTTTAT TCTTTAGTGT CAAAGATATC TCCTGACTGG 
ATTAGCTCCA TGAGTATTAT TCGATCTCAC CGAACACAAG AGGATACTGT TGTAGCAGTT 
TCAATGACAG GAATTTTGAA GGTTTGGATT GTTACATCTG AAATTAGTGA TACCCAGGAT 
ACGGAGCCAA TATTTGAAGA AGAATCCAAA CCAATTTATT GTCAGAATTG CCAAAGTATC 
TCTTTCTGTG CATTCACCCA AAGGTCACTT TTGGTTGTGT GTTCCAAATA TTGGAGGGTA 
TTTGATGCTG GAGATTATTC TCTATTATGC TCTGGCCCCA GTGAAAATGG ACAAACATGG 
ACAGGTGGTG ACTTCATATC AGCAGATAAA GTAATTATAT GGACAGAAAA TGGACAGAGC 
TATATTTACA AACTACCTGC CAGTTGCCTG CCTGCTAGTG ATTCATTTCG CAGTGATGTT 
GGAAAGGCTG TTGAAAATTT AATACCTCCT GTACAGTATG GTCTCTTGGA TAGAGAAGAT 
AAAGAGTTGT TTATCTGCCC TCCTGTAACT CGATTCTTTT ATGGACGTAG GGAATGCTTC 
CATAAGCTAC TTATTCAGGG AGACTCTTCA GGAAGACTAT GTATTTGGAA TGTACCAGAC 
ACCCTTGAAA AACAGGAAGC TGAAGAAGGA TTGAAAATGA CAACTTCAAT TTGTTTACAA 
GAGGCCTTTG GTAAACTCAC TCCTTGTCCT GCTGGTATTA TAGATCAACT AAGTTTGGTT 
CCTAATAGCA GTGACCCTTT GAAAGTTACA GCTAGTGTGT ATATCCCATC CCATGGACGA 
CTAGTTTGTG GTCGTGAAGA TGGAAGCATA ATTATTGTGC CAGCAACACA AACTGCTATA 
GTCCAGCTGT TACAAGGGGA ACATATGCTC CGGAGAGGTT GGCCACCTCA CAGAACTCTT 
CGGGGTCATC GGAACAAAAT TACATGTTTA CTATATCCTC ATCAGGTCTC AGCTCGTTAT 
GACCAAAGAT ACTTGATTTC TGGAGGGGTA GATTTTTCTG TCATAATCTG GGATATATTT 
TCTGGAGAAA TGAAGCATAT CTTCTGTGTG CATGGTGGAG AGATTACTCA ACTCCTTGTT 
CCACCTGAAA ACTGCAGTAC AAGAGTGCAA CACTGCATCT GCTCTGTTGC CAGTGACCAT 
TCTGTAGGAC TTCTAAGTTT GAGAGAAAAG AAATGTATCA TGTTGGCATC TCGTCACCTT 
TTTCCCATCC AAGTAATAAA ATGGAGGCCT TCAGATGATT ATCTAGTGGT TGGCTGTTCA 
GATGGCTCTG TGTATGTCTG GCAAATGGAT ACTGGTGCAC TGGATCGTTG TGTGATGGGG 
ATTACTGCAG TTGAGATTTT GAATGCTTGT GATGAAGCAG TTCCTGCTGC AGTTGATTCT 
CTTAGTCATC CAGCTATTAA TTTAAAACAG GCTATGACAA GACGTAGTCT TGCTGCTTTA 
AAAAATATGG CTCATCATAA GTTACAGACT CTTGCCACTA ACCTCTTGGC TTCTGAAGCT 
TCCGACAAAG GAAATTTACC TAAGTATTCT CATAATTCTC TAATGGTTCA AGCAATAAAG 
ACTAACCTAA CAGATCCGGA CATACATGTG CTCTTCTTTG ATGTGGAAGC TTTGATTATT 
CAGCTACTGA CTGAAGAAGC CTCTAGGCCG AATACTGCCC TTATTTCCCC AGAGAGTTTG 
CATAAAGCAT CAGGCAGTGC TGACAAAGGG GGCTCTTTCC TGACTGGCAA AAGAGCTGCC 
GTACTCTTCC AGCAAGTCAA AGAAACAATC AAAGAGAACA TAAAGGAGCA TCTTCTTGAT 
GATGAAGATG AGGACGAGGA GATACTGAGG CAGAAGAGAG AAGAAAGTGA CCCAGAGTAT 
CGATCTAGCA AATCTAAGCC ATTGACTTTA TTAGAGTATA ACCTAACAAT GGATACTGCC 
AAGTTGTTTA TGTCCTGTCT TCATGCCTGG GGTTTGAATG AAGTTCTAGA TGAAGTTTGT 
CTTGACCGCC TTGGAATGCT TAAACCCCAC TGTACAGTCT CCTTTGGTCT TCTGTCAAGA 
GGAGGCCACA TGTCATTGAT GCTTCCTGGT CATAACCAGC CTATATGTAA GCCATCCTGT 
GGAAAACCAG AAGGGGAAAG AAAACTCTCA ACCACAGATG GAGTAGGAAA AGGAACCTAT 
GGAGTATCCC GGGCTGTTAC CACTCAGCAC CTTCTGTCTA TTATATCTTT GGCAAACACC 
TTAATGAGTA TGACCAATGC AACTTTTATT GGTGATCATA TGAAGAAGGG CCCTGTCAGG 
CCACCTAGGC CAAGCACCCC AGACTTTTCC AAAGTGAAGG GTTCCCCTCC AACTTCCAGT 
AATGTTGTTC AAGGACAGAT TAAACAAGGA TGGAGTCAAC TAGCTGCTAT GCATTGTGTT 
ATGCTTCCAG ACCTGTTGGG ATTGGATAAA TTTAGACCTC CACTTCTAGA AATGCTGGCT 
CGGAGATGGC AGGATCGATG CCTGGAGGTA AGAGAAGCGG CTCAAGCTTT ACTGTTGGCA 
GAACTGAGAA GAATTGAACA AGGAGGAAGA AAAGAAACAA TTGATGCTTG GGCTCCTTAT 
TTACCTCAGT ATATGGATAG TGTTATATCA CCTGGAGTGA CAGCAGAATC CATTCAGACT 
GCAAATGCTA GTCCAGATGC CTTGGGGACT GAAACTAAAG TCCAAGAAGA AGAGCATGAT 
CTTGTTGATG ATGACATCAC AGCAGGGTGT CTATCATCAG GTCTCCCACA CATGAAGAAA 
ATATCCACAT CATATGAAGA AAGACGTAAA CAAGCTACAG CTATTGTTTT ATTAGGAGTT 
ATAGGAGCCG AATTTGGTGC AGAAATTGAA CCTCCGAAAT TGTTGACAAG ACCTCGAAGT 
TCCAGCCAAA TTCCCGAAGG GTTTGGTTTG ACCAGTGGTG GATCCAACTA TTCATTAGCC 
AGACATACTT GCAAGGCATT GACGTTTCTT CTCCTACAAC CTCCTAGTCC TAAACTACCA 
CCTCACAGCA CAATCAGAAG AACTGCTATT GATCTAATAG GACGAGGTTT TACAGTATGG 
GAGCCTTATA TGGATGTGTC TGCTGTTCTA ATGGGCCTTC TCGAACTCTG TGCTGATGCT 
GAGAAGCAAC TGGCTAACAT CACAATGGGG CTGCCCCTGA GTCCAGCAGC TGACTCCGCC 
CGCTCCGCAA GACATGCCCT CTCACTCATT GCCACTGCCA GACCACCCGC CTTCATCACA 
ACTATTGCCA AGGAGGTACA TCGACATACT GCTCTAGCAG CAAACACCCA GTCACAGCAG 
AATATTCATA CTACAACTCT TGCTCGAGCT AAAGGGGAGA TTCTGAGAGT CATTGAAATT 
CTTATTGAAA AAATGCCCAC AGATGTGGTT GATCTACTTG TTGAGGTTAT GGACATTATA 
ATGTATTGTC TTGAAGGATC TTTAGTTAAA AAGAAGGGAC TTCAAGAATG TTTCCCAGCC 
ATCTGCAGGT TCTACATGGT CAGCTATTAT GAACGAAATC ACAGAATAGC AGTTGGAGCT 
CGCCATGGTT CAGTGGCCCT GTACGACATC CGGACTGGAA AATGTCAGAC AATTCATGGG 
CACAAGGGGC CAATAACTGC TGTGGCATTT GCTCCTGATG GAAGGTATCT TGCCACCTAC 
TCAAATTCTG ACAGTCACAT TTCCTTCTGG CAGATGAACA CCTCACTTTT AGGAAGTATT 
GGTATGCTGA ACTCAGCACC TCAGCTTCGT TGCATTAAAA CTTACCAAGT GCCCCCTGTC 
CAACCTGCAT CCCCTGGATC ACATAATGCA CTCAGACTCG CCCGGCTGAT CTGGACTTCA 
AATCGCAATG TTATTCTTAT GGCCCATGAT GGAAAGGAGC ATCGATTTAT GGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031801786.1
CDS	284..4660
Translation	MAGNSLVLPIVLWGRKAPTHCISAVLLMDDGATIVTGCHDGQICLWDLSLELEINPRALLFGHTASITCLSKACASSDRQYVVSASESGEMCLWDVSDGRCIEFTKLACTHTGIEFYQFTVGNQREGRLLCHGHYPEILVVDATSLEVLYSLVSKISPDWISSMSIIRSHRTQEDTVVAVSMTGILKVWIVTSEISDTQDTEPIFEEESKPIYCQNCQSISFCAFTQRSLLVVCSKYWRVFDAGDYSLLCSGPSENGQTWTGGDFISADKVIIWTENGQSYIYKLPASCLPASDSFRSDVGKAVENLIPPVQYGLLDREDKELFICPPVTRFFYGRRECFHKLLIQGDSSGRLCIWNVPDTLEKQEAEEGLKMTTSICLQEAFGKLTPCPAGIIDQLSLVPNSSDPLKVTASVYIPSHGRLVCGREDGSIIIVPATQTAIVQLLQGEHMLRRGWPPHRTLRGHRNKITCLLYPHQVSARYDQRYLISGGVDFSVIIWDIFSGEMKHIFCVHGGEITQLLVPPENCSTRVQHCICSVASDHSVGLLSLREKKCIMLASRHLFPIQVIKWRPSDDYLVVGCSDGSVYVWQMDTGALDRCVMGITAVEILNACDEAVPAAVDSLSHPAINLKQAMTRRSLAALKNMAHHKLQTLATNLLASEASDKGNLPKYSHNSLMVQAIKTNLTDPDIHVLFFDVEALIIQLLTEEASRPNTALISPESLHKASGSADKGGSFLTGKRAAVLFQQVKETIKENIKEHLLDDEDEDEEILRQKREESDPEYRSSKSKPLTLLEYNLTMDTAKLFMSCLHAWGLNEVLDEVCLDRLGMLKPHCTVSFGLLSRGGHMSLMLPGHNQPICKPSCGKPEGERKLSTTDGVGKGTYGVSRAVTTQHLLSIISLANTLMSMTNATFIGDHMKKGPVRPPRPSTPDFSKVKGSPPTSSNVVQGQIKQGWSQLAAMHCVMLPDLLGLDKFRPPLLEMLARRWQDRCLEVREAAQALLLAELRRIEQGGRKETIDAWAPYLPQYMDSVISPGVTAESIQTANASPDALGTETKVQEEEHDLVDDDITAGCLSSGLPHMKKISTSYEERRKQATAIVLLGVIGAEFGAEIEPPKLLTRPRSSSQIPEGFGLTSGGSNYSLARHTCKALTFLLLQPPSPKLPPHSTIRRTAIDLIGRGFTVWEPYMDVSAVLMGLLELCADAEKQLANITMGLPLSPAADSARSARHALSLIATARPPAFITTIAKEVHRHTALAANTQSQQNIHTTTLARAKGEILRVIEILIEKMPTDVVDLLVEVMDIIMYCLEGSLVKKKGLQECFPAICRFYMVSYYERNHRIAVGARHGSVALYDIRTGKCQTIHGHKGPITAVAFAPDGRYLATYSNSDSHISFWQMNTSLLGSIGMLNSAPQLRCIKTYQVPPVQPASPGSHNALRLARLIWTSNRNVILMAHDGKEHRFMV

Target ORF information:

RefSeq Version	XM_031945926.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WD repeat domain 7 (WDR7), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031945926.1

ORF Insert Sequence:

ATGGCAGGAA ATAGCCTTGT TCTACCAATT GTTCTTTGGG GCCGTAAAGC ACCAACACAT 
TGCATCTCAG CTGTGCTTTT AATGGATGAT GGGGCTACAA TTGTAACAGG GTGTCATGAT 
GGACAAATAT GTCTCTGGGA TCTTTCCTTA GAATTGGAAA TCAATCCTCG GGCTCTCTTG 
TTTGGCCATA CAGCTTCAAT TACTTGTTTG TCGAAAGCCT GTGCTTCTAG TGACCGTCAG 
TATGTAGTGA GTGCATCTGA AAGTGGAGAA ATGTGCCTTT GGGATGTAAG TGATGGCAGA 
TGTATTGAGT TTACAAAATT AGCTTGCACA CATACTGGCA TAGAGTTCTA CCAGTTCACT 
GTTGGGAATC AGCGTGAAGG GAGACTGCTG TGCCATGGAC ATTACCCTGA AATTCTGGTT 
GTGGATGCCA CCAGCCTTGA GGTTCTTTAT TCTTTAGTGT CAAAGATATC TCCTGACTGG 
ATTAGCTCCA TGAGTATTAT TCGATCTCAC CGAACACAAG AGGATACTGT TGTAGCAGTT 
TCAATGACAG GAATTTTGAA GGTTTGGATT GTTACATCTG AAATTAGTGA TACCCAGGAT 
ACGGAGCCAA TATTTGAAGA AGAATCCAAA CCAATTTATT GTCAGAATTG CCAAAGTATC 
TCTTTCTGTG CATTCACCCA AAGGTCACTT TTGGTTGTGT GTTCCAAATA TTGGAGGGTA 
TTTGATGCTG GAGATTATTC TCTATTATGC TCTGGCCCCA GTGAAAATGG ACAAACATGG 
ACAGGTGGTG ACTTCATATC AGCAGATAAA GTAATTATAT GGACAGAAAA TGGACAGAGC 
TATATTTACA AACTACCTGC CAGTTGCCTG CCTGCTAGTG ATTCATTTCG CAGTGATGTT 
GGAAAGGCTG TTGAAAATTT AATACCTCCT GTACAGTATG GTCTCTTGGA TAGAGAAGAT 
AAAGAGTTGT TTATCTGCCC TCCTGTAACT CGATTCTTTT ATGGACGTAG GGAATGCTTC 
CATAAGCTAC TTATTCAGGG AGACTCTTCA GGAAGACTAT GTATTTGGAA TGTACCAGAC 
ACCCTTGAAA AACAGGAAGC TGAAGAAGGA TTGAAAATGA CAACTTCAAT TTGTTTACAA 
GAGGCCTTTG GTAAACTCAC TCCTTGTCCT GCTGGTATTA TAGATCAACT AAGTTTGGTT 
CCTAATAGCA GTGACCCTTT GAAAGTTACA GCTAGTGTGT ATATCCCATC CCATGGACGA 
CTAGTTTGTG GTCGTGAAGA TGGAAGCATA ATTATTGTGC CAGCAACACA AACTGCTATA 
GTCCAGCTGT TACAAGGGGA ACATATGCTC CGGAGAGGTT GGCCACCTCA CAGAACTCTT 
CGGGGTCATC GGAACAAAAT TACATGTTTA CTATATCCTC ATCAGGTCTC AGCTCGTTAT 
GACCAAAGAT ACTTGATTTC TGGAGGGGTA GATTTTTCTG TCATAATCTG GGATATATTT 
TCTGGAGAAA TGAAGCATAT CTTCTGTGTG CATGGTGGAG AGATTACTCA ACTCCTTGTT 
CCACCTGAAA ACTGCAGTAC AAGAGTGCAA CACTGCATCT GCTCTGTTGC CAGTGACCAT 
TCTGTAGGAC TTCTAAGTTT GAGAGAAAAG AAATGTATCA TGTTGGCATC TCGTCACCTT 
TTTCCCATCC AAGTAATAAA ATGGAGGCCT TCAGATGATT ATCTAGTGGT TGGCTGTTCA 
GATGGCTCTG TGTATGTCTG GCAAATGGAT ACTGGTGCAC TGGATCGTTG TGTGATGGGG 
ATTACTGCAG TTGAGATTTT GAATGCTTGT GATGAAGCAG TTCCTGCTGC AGTTGATTCT 
CTTAGTCATC CAGCTATTAA TTTAAAACAG GCTATGACAA GACGTAGTCT TGCTGCTTTA 
AAAAATATGG CTCATCATAA GTTACAGACT CTTGCCACTA ACCTCTTGGC TTCTGAAGCT 
TCCGACAAAG GAAATTTACC TAAGTATTCT CATAATTCTC TAATGGTTCA AGCAATAAAG 
ACTAACCTAA CAGATCCGGA CATACATGTG CTCTTCTTTG ATGTGGAAGC TTTGATTATT 
CAGCTACTGA CTGAAGAAGC CTCTAGGCCG AATACTGCCC TTATTTCCCC AGAGAGTTTG 
CATAAAGCAT CAGGCAGTGC TGACAAAGGG GGCTCTTTCC TGACTGGCAA AAGAGCTGCC 
GTACTCTTCC AGCAAGTCAA AGAAACAATC AAAGAGAACA TAAAGGAGCA TCTTCTTGAT 
GATGAAGATG AGGACGAGGA GATACTGAGG CAGAAGAGAG AAGAAAGTGA CCCAGAGTAT 
CGATCTAGCA AATCTAAGCC ATTGACTTTA TTAGAGTATA ACCTAACAAT GGATACTGCC 
AAGTTGTTTA TGTCCTGTCT TCATGCCTGG GGTTTGAATG AAGTTCTAGA TGAAGTTTGT 
CTTGACCGCC TTGGAATGCT TAAACCCCAC TGTACAGTCT CCTTTGGTCT TCTGTCAAGA 
GGAGGCCACA TGTCATTGAT GCTTCCTGGT CATAACCAGC CTATATGTAA GCCATCCTGT 
GGAAAACCAG AAGGGGAAAG AAAACTCTCA ACCACAGATG GAGTAGGAAA AGGAACCTAT 
GGAGTATCCC GGGCTGTTAC CACTCAGCAC CTTCTGTCTA TTATATCTTT GGCAAACACC 
TTAATGAGTA TGACCAATGC AACTTTTATT GGTGATCATA TGAAGAAGGG CCCTGTCAGG 
CCACCTAGGC CAAGCACCCC AGACTTTTCC AAAGTGAAGG GTTCCCCTCC AACTTCCAGT 
AATGTTGTTC AAGGACAGAT TAAACAAGGA TGGAGTCAAC TAGCTGCTAT GCATTGTGTT 
ATGCTTCCAG ACCTGTTGGG ATTGGATAAA TTTAGACCTC CACTTCTAGA AATGCTGGCT 
CGGAGATGGC AGGATCGATG CCTGGAGGTA AGAGAAGCGG CTCAAGCTTT ACTGTTGGCA 
GAACTGAGAA GAATTGAACA AGGAGGAAGA AAAGAAACAA TTGATGCTTG GGCTCCTTAT 
TTACCTCAGT ATATGGATAG TGTTATATCA CCTGGAGTGA CAGCAGAATC CATTCAGACT 
GCAAATGCTA GTCCAGATGC CTTGGGGACT GAAACTAAAG TCCAAGAAGA AGAGCATGAT 
CTTGTTGATG ATGACATCAC AGCAGGGTGT CTATCATCAG GTCTCCCACA CATGAAGAAA 
ATATCCACAT CATATGAAGA AAGACGTAAA CAAGCTACAG CTATTGTTTT ATTAGGAGTT 
ATAGGAGCCG AATTTGGTGC AGAAATTGAA CCTCCGAAAT TGTTGACAAG ACCTCGAAGT 
TCCAGCCAAA TTCCCGAAGG GTTTGGTTTG ACCAGTGGTG GATCCAACTA TTCATTAGCC 
AGACATACTT GCAAGGCATT GACGTTTCTT CTCCTACAAC CTCCTAGTCC TAAACTACCA 
CCTCACAGCA CAATCAGAAG AACTGCTATT GATCTAATAG GACGAGGTTT TACAGTATGG 
GAGCCTTATA TGGATGTGTC TGCTGTTCTA ATGGGCCTTC TCGAACTCTG TGCTGATGCT 
GAGAAGCAAC TGGCTAACAT CACAATGGGG CTGCCCCTGA GTCCAGCAGC TGACTCCGCC 
CGCTCCGCAA GACATGCCCT CTCACTCATT GCCACTGCCA GACCACCCGC CTTCATCACA 
ACTATTGCCA AGGAGGTACA TCGACATACT GCTCTAGCAG CAAACACCCA GTCACAGCAG 
AATATTCATA CTACAACTCT TGCTCGAGCT AAAGGGGAGA TTCTGAGAGT CATTGAAATT 
CTTATTGAAA AAATGCCCAC AGATGTGGTT GATCTACTTG TTGAGGTTAT GGACATTATA 
ATGTATTGTC TTGAAGGATC TTTAGTTAAA AAGAAGGGAC TTCAAGAATG TTTCCCAGCC 
ATCTGCAGGT TCTACATGGT CAGCTATTAT GAACGAAATC ACAGAATAGC AGTTGGAGCT 
CGCCATGGTT CAGTGGCCCT GTACGACATC CGGACTGGAA AATGTCAGAC AATTCATGGG 
CACAAGGGGC CAATAACTGC TGTGGCATTT GCTCCTGATG GAAGGTATCT TGCCACCTAC 
TCAAATTCTG ACAGTCACAT TTCCTTCTGG CAGATGAACA CCTCACTTTT AGGAAGTATT 
GGTATGCTGA ACTCAGCACC TCAGCTTCGT TGCATTAAAA CTTACCAAGT GCCCCCTGTC 
CAACCTGCAT CCCCTGGATC ACATAATGCA CTCAGACTCG CCCGGCTGAT CTGGACTTCA 
AATCGCAATG TTATTCTTAT GGCCCATGAT GGAAAGGAGC ATCGATTTAT GGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100927741 ( XM_031945927.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031801787.1 Sarcophilus harrisii WD repeat domain 7 (WDR7), transcript variant X6, mRNA.

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CloneID	OSa220935
Clone ID Related Accession (Same CDS sequence)	XM_031945927.1
Accession Version	XM_031945927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	WD repeat-containing protein 7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA ATAGCCTTGT TCTACCAATT GTTCTTTGGG GCCGTAAAGC ACCAACACAT 
TGCATCTCAG CTGTGCTTTT AATGGATGAT GGGGCTACAA TTGTAACAGG GTGTCATGAT 
GGACAAATAT GTCTCTGGGA TCTTTCCTTA GAATTGGAAA TCAATCCTCG GGCTCTCTTG 
TTTGGCCATA CAGCTTCAAT TACTTGTTTG TCGAAAGCCT GTGCTTCTAG TGACCGTCAG 
TATGTAGTGA GTGCATCTGA AAGTGGAGAA ATGTGCCTTT GGGATGTAAG TGATGGCAGA 
TGTATTGAGT TTACAAAATT AGCTTGCACA CATACTGGCA TAGAGTTCTA CCAGTTCACT 
GTTGGGAATC AGCGTGAAGG GAGACTGCTG TGCCATGGAC ATTACCCTGA AATTCTGGTT 
GTGGATGCCA CCAGCCTTGA GGTTCTTTAT TCTTTAGTGT CAAAGATATC TCCTGACTGG 
ATTAGCTCCA TGAGTATTAT TCGATCTCAC CGAACACAAG AGGATACTGT TGTAGCAGTT 
TCAATGACAG GAATTTTGAA GGTTTGGATT GTTACATCTG AAATTAGTGA TACCCAGGAT 
ACGGAGCCAA TATTTGAAGA AGAATCCAAA CCAATTTATT GTCAGAATTG CCAAAGTATC 
TCTTTCTGTG CATTCACCCA AAGGTCACTT TTGGTTGTGT GTTCCAAATA TTGGAGGGTA 
TTTGATGCTG GAGATTATTC TCTATTATGC TCTGGCCCCA GTGAAAATGG ACAAACATGG 
ACAGGTGGTG ACTTCATATC AGCAGATAAA GTAATTATAT GGACAGAAAA TGGACAGAGC 
TATATTTACA AACTACCTGC CAGTTGCCTG CCTGCTAGTG ATTCATTTCG CAGTGATGTT 
GGAAAGGCTG TTGAAAATTT AATACCTCCT GTACAGTATG GTCTCTTGGA TAGAGAAGAT 
AAAGAGTTGT TTATCTGCCC TCCTGTAACT CGATTCTTTT ATGGACGTAG GGAATGCTTC 
CATAAGCTAC TTATTCAGGG AGACTCTTCA GGAAGACTAT GTATTTGGAA TGTACCAGAC 
ACCCTTGAAA AACAGGAAGC TGAAGAAGGA TTGAAAATGA CAACTTCAAT TTGTTTACAA 
GAGGCCTTTG GTAAACTCAC TCCTTGTCCT GCTGGTATTA TAGATCAACT AAGTTTGGTT 
CCTAATAGCA GTGACCCTTT GAAAGTTACA GCTAGTGTGT ATATCCCATC CCATGGACGA 
CTAGTTTGTG GTCGTGAAGA TGGAAGCATA ATTATTGTGC CAGCAACACA AACTGCTATA 
GTCCAGCTGT TACAAGGGGA ACATATGCTC CGGAGAGGTT GGCCACCTCA CAGAACTCTT 
CGGGGTCATC GGAACAAAAT TACATGTTTA CTATATCCTC ATCAGGTCTC AGCTCGTTAT 
GACCAAAGAT ACTTGATTTC TGGAGGGGTA GATTTTTCTG TCATAATCTG GGATATATTT 
TCTGGAGAAA TGAAGCATAT CTTCTGTGTG CATGGTGGAG AGATTACTCA ACTCCTTGTT 
CCACCTGAAA ACTGCAGTAC AAGAGTGCAA CACTGCATCT GCTCTGTTGC CAGTGACCAT 
TCTGTAGGAC TTCTAAGTTT GAGAGAAAAG AAATGTATCA TGTTGGCATC TCGTCACCTT 
TTTCCCATCC AAGTAATAAA ATGGAGGCCT TCAGATGATT ATCTAGTGGT TGGCTGTTCA 
GATGGCTCTG TGTATGTCTG GCAAATGGAT ACTGGTGCAC TGGATCGTTG TGTGATGGGG 
ATTACTGCAG TTGAGATTTT GAATGCTTGT GATGAAGCAG TTCCTGCTGC AGTTGATTCT 
CTTAGTCATC CAGCTATTAA TTTAAAACAG GCTATGACAA GACGTAGTCT TGCTGCTTTA 
AAAAATATGG CTCATCATAA GTTACAGACT CTTGCCACTA ACCTCTTGGC TTCTGAAGCT 
TCCGACAAAG GAAATTTACC TAAGTATTCT CATAATTCTC TAATGGTTCA AGCAATAAAG 
ACTAACCTAA CAGATCCGGA CATACATGTG CTCTTCTTTG ATGTGGAAGC TTTGATTATT 
CAGCTACTGA CTGAAGAAGC CTCTAGGCCG AATACTGCCC TTATTTCCCC AGAGAGTTTG 
CATAAAGCAT CAGGCAGTGC TGACAAAGGG GGCTCTTTCC TGACTGGCAA AAGAGCTGCC 
GTACTCTTCC AGCAAGTCAA AGAAACAATC AAAGAGAACA TAAAGGAGCA TCTTCTTGAT 
GATGAAGATG AGGACGAGGA GATACTGAGG CAGAAGAGAG AAGAAAGTGA CCCAGAGTAT 
CGATCTAGCA AATCTAAGCC ATTGACTTTA TTAGAGTATA ACCTAACAAT GGATACTGCC 
AAGTTGTTTA TGTCCTGTCT TCATGCCTGG GGTTTGAATG AAGTTCTAGA TGAAGTTTGT 
CTTGACCGCC TTGGAATGCT TAAACCCCAC TGTACAGTCT CCTTTGGTCT TCTGTCAAGA 
GGAGGCCACA TGTCATTGAT GCTTCCTGGT CATAACCAGC CTATATGTAA GCCATCCTGT 
GGAAAACCAG AAGGGGAAAG AAAACTCTCA ACCACAGATG GAGTAGGAAA AGGAACCTAT 
GGAGTATCCC GGGCTGTTAC CACTCAGCAC CTTCTGTCTA TTATATCTTT GGCAAACACC 
TTAATGAGTA TGACCAATGC AACTTTTATT GGTGATCATA TGAAGAAGGG CCCTGTCAGG 
CCACCTAGGC CAAGCACCCC AGACTTTTCC AAAGTGAAGG GTTCCCCTCC AACTTCCAGT 
AATGTTGTTC AAGGACAGAT TAAACAAGCT GCTGCACCTG TCGTTTCCGC TCGGTCTGAT 
ACTGATCACT CTGGTTCTGA CCCTGCTCCT ACTCCTGCTT TACATGCCTG TTTCTTAGTA 
AATGAAGGAT GGAGTCAACT AGCTGCTATG CATTGTGTTA TGCTTCCAGA CCTGTTGGGA 
TTGGATAAAT TTAGACCTCC ACTTCTAGAA ATGCTGGCTC GGAGATGGCA GGATCGATGC 
CTGGAGGTAA GAGAAGCGGC TCAAGCTTTA CTGTTGGCAG AACTGAGAAG AATTGAACAA 
GGAGGAAGAA AAGAAACAAT TGATGCTTGG GCTCCTTATT TACCTCAGTA TATGGATAGT 
GTTATATCAC CTGGAGTGAC AGCAGAATCC ATTCAGACTG CAAATGCTAG TCCAGATGCC 
TTGGGGACTG AAACTAAAGT CCAAGAAGAA GAGCATGATC TTGTTGATGA TGACATCACA 
GCAGGGTGTC TATCATCAGG TCTCCCACAC ATGAAGAAAA TATCCACATC ATATGAAGAA 
AGACGTAAAC AAGCTACAGC TATTGTTTTA TTAGGAGTTA TAGGAGCCGA ATTTGGTGCA 
GAAATTGAAC CTCCGAAATT GTTGACAAGA CCTCGAAGTT CCAGCCAAAT TCCCGAAGGG 
TTTGGTTTGA CCAGTGGTGG ATCCAACTAT TCATTAGCCA GACATACTTG CAAGGCATTG 
ACGTTTCTTC TCCTACAACC TCCTAGTCCT AAACTACCAC CTCACAGCAC AATCAGAAGA 
ACTGCTATTG ATCTAATAGG ACGAGGTTTT ACAGTATGGG AGCCTTATAT GGATGTGTCT 
GCTGTTCTAA TGGGCCTTCT CGAACTCTGT GCTGATGCTG AGAAGCAACT GGCTAACATC 
ACAATGGGGC TGCCCCTGAG TCCAGCAGCT GACTCCGCCC GCTCCGCAAG ACATGCCCTC 
TCACTCATTG CCACTGCCAG ACCACCCGCC TTCATCACAA CTATTGCCAA GGAGGCCAGC 
ATATGCAGTC CTTCATATGC TAAGATGATG TTCCTGAGTA CATCGACATA CTGCTCTAGC 
AGCAAACACC CAGTCACAGC AGAATATTCA TACTACAACT CTTGCTCGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031801787.1
CDS	286..4239
Translation	MAGNSLVLPIVLWGRKAPTHCISAVLLMDDGATIVTGCHDGQICLWDLSLELEINPRALLFGHTASITCLSKACASSDRQYVVSASESGEMCLWDVSDGRCIEFTKLACTHTGIEFYQFTVGNQREGRLLCHGHYPEILVVDATSLEVLYSLVSKISPDWISSMSIIRSHRTQEDTVVAVSMTGILKVWIVTSEISDTQDTEPIFEEESKPIYCQNCQSISFCAFTQRSLLVVCSKYWRVFDAGDYSLLCSGPSENGQTWTGGDFISADKVIIWTENGQSYIYKLPASCLPASDSFRSDVGKAVENLIPPVQYGLLDREDKELFICPPVTRFFYGRRECFHKLLIQGDSSGRLCIWNVPDTLEKQEAEEGLKMTTSICLQEAFGKLTPCPAGIIDQLSLVPNSSDPLKVTASVYIPSHGRLVCGREDGSIIIVPATQTAIVQLLQGEHMLRRGWPPHRTLRGHRNKITCLLYPHQVSARYDQRYLISGGVDFSVIIWDIFSGEMKHIFCVHGGEITQLLVPPENCSTRVQHCICSVASDHSVGLLSLREKKCIMLASRHLFPIQVIKWRPSDDYLVVGCSDGSVYVWQMDTGALDRCVMGITAVEILNACDEAVPAAVDSLSHPAINLKQAMTRRSLAALKNMAHHKLQTLATNLLASEASDKGNLPKYSHNSLMVQAIKTNLTDPDIHVLFFDVEALIIQLLTEEASRPNTALISPESLHKASGSADKGGSFLTGKRAAVLFQQVKETIKENIKEHLLDDEDEDEEILRQKREESDPEYRSSKSKPLTLLEYNLTMDTAKLFMSCLHAWGLNEVLDEVCLDRLGMLKPHCTVSFGLLSRGGHMSLMLPGHNQPICKPSCGKPEGERKLSTTDGVGKGTYGVSRAVTTQHLLSIISLANTLMSMTNATFIGDHMKKGPVRPPRPSTPDFSKVKGSPPTSSNVVQGQIKQAAAPVVSARSDTDHSGSDPAPTPALHACFLVNEGWSQLAAMHCVMLPDLLGLDKFRPPLLEMLARRWQDRCLEVREAAQALLLAELRRIEQGGRKETIDAWAPYLPQYMDSVISPGVTAESIQTANASPDALGTETKVQEEEHDLVDDDITAGCLSSGLPHMKKISTSYEERRKQATAIVLLGVIGAEFGAEIEPPKLLTRPRSSSQIPEGFGLTSGGSNYSLARHTCKALTFLLLQPPSPKLPPHSTIRRTAIDLIGRGFTVWEPYMDVSAVLMGLLELCADAEKQLANITMGLPLSPAADSARSARHALSLIATARPPAFITTIAKEASICSPSYAKMMFLSTSTYCSSSKHPVTAEYSYYNSCSS

Target ORF information:

RefSeq Version	XM_031945927.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WD repeat domain 7 (WDR7), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031945927.1

ORF Insert Sequence:

ATGGCAGGAA ATAGCCTTGT TCTACCAATT GTTCTTTGGG GCCGTAAAGC ACCAACACAT 
TGCATCTCAG CTGTGCTTTT AATGGATGAT GGGGCTACAA TTGTAACAGG GTGTCATGAT 
GGACAAATAT GTCTCTGGGA TCTTTCCTTA GAATTGGAAA TCAATCCTCG GGCTCTCTTG 
TTTGGCCATA CAGCTTCAAT TACTTGTTTG TCGAAAGCCT GTGCTTCTAG TGACCGTCAG 
TATGTAGTGA GTGCATCTGA AAGTGGAGAA ATGTGCCTTT GGGATGTAAG TGATGGCAGA 
TGTATTGAGT TTACAAAATT AGCTTGCACA CATACTGGCA TAGAGTTCTA CCAGTTCACT 
GTTGGGAATC AGCGTGAAGG GAGACTGCTG TGCCATGGAC ATTACCCTGA AATTCTGGTT 
GTGGATGCCA CCAGCCTTGA GGTTCTTTAT TCTTTAGTGT CAAAGATATC TCCTGACTGG 
ATTAGCTCCA TGAGTATTAT TCGATCTCAC CGAACACAAG AGGATACTGT TGTAGCAGTT 
TCAATGACAG GAATTTTGAA GGTTTGGATT GTTACATCTG AAATTAGTGA TACCCAGGAT 
ACGGAGCCAA TATTTGAAGA AGAATCCAAA CCAATTTATT GTCAGAATTG CCAAAGTATC 
TCTTTCTGTG CATTCACCCA AAGGTCACTT TTGGTTGTGT GTTCCAAATA TTGGAGGGTA 
TTTGATGCTG GAGATTATTC TCTATTATGC TCTGGCCCCA GTGAAAATGG ACAAACATGG 
ACAGGTGGTG ACTTCATATC AGCAGATAAA GTAATTATAT GGACAGAAAA TGGACAGAGC 
TATATTTACA AACTACCTGC CAGTTGCCTG CCTGCTAGTG ATTCATTTCG CAGTGATGTT 
GGAAAGGCTG TTGAAAATTT AATACCTCCT GTACAGTATG GTCTCTTGGA TAGAGAAGAT 
AAAGAGTTGT TTATCTGCCC TCCTGTAACT CGATTCTTTT ATGGACGTAG GGAATGCTTC 
CATAAGCTAC TTATTCAGGG AGACTCTTCA GGAAGACTAT GTATTTGGAA TGTACCAGAC 
ACCCTTGAAA AACAGGAAGC TGAAGAAGGA TTGAAAATGA CAACTTCAAT TTGTTTACAA 
GAGGCCTTTG GTAAACTCAC TCCTTGTCCT GCTGGTATTA TAGATCAACT AAGTTTGGTT 
CCTAATAGCA GTGACCCTTT GAAAGTTACA GCTAGTGTGT ATATCCCATC CCATGGACGA 
CTAGTTTGTG GTCGTGAAGA TGGAAGCATA ATTATTGTGC CAGCAACACA AACTGCTATA 
GTCCAGCTGT TACAAGGGGA ACATATGCTC CGGAGAGGTT GGCCACCTCA CAGAACTCTT 
CGGGGTCATC GGAACAAAAT TACATGTTTA CTATATCCTC ATCAGGTCTC AGCTCGTTAT 
GACCAAAGAT ACTTGATTTC TGGAGGGGTA GATTTTTCTG TCATAATCTG GGATATATTT 
TCTGGAGAAA TGAAGCATAT CTTCTGTGTG CATGGTGGAG AGATTACTCA ACTCCTTGTT 
CCACCTGAAA ACTGCAGTAC AAGAGTGCAA CACTGCATCT GCTCTGTTGC CAGTGACCAT 
TCTGTAGGAC TTCTAAGTTT GAGAGAAAAG AAATGTATCA TGTTGGCATC TCGTCACCTT 
TTTCCCATCC AAGTAATAAA ATGGAGGCCT TCAGATGATT ATCTAGTGGT TGGCTGTTCA 
GATGGCTCTG TGTATGTCTG GCAAATGGAT ACTGGTGCAC TGGATCGTTG TGTGATGGGG 
ATTACTGCAG TTGAGATTTT GAATGCTTGT GATGAAGCAG TTCCTGCTGC AGTTGATTCT 
CTTAGTCATC CAGCTATTAA TTTAAAACAG GCTATGACAA GACGTAGTCT TGCTGCTTTA 
AAAAATATGG CTCATCATAA GTTACAGACT CTTGCCACTA ACCTCTTGGC TTCTGAAGCT 
TCCGACAAAG GAAATTTACC TAAGTATTCT CATAATTCTC TAATGGTTCA AGCAATAAAG 
ACTAACCTAA CAGATCCGGA CATACATGTG CTCTTCTTTG ATGTGGAAGC TTTGATTATT 
CAGCTACTGA CTGAAGAAGC CTCTAGGCCG AATACTGCCC TTATTTCCCC AGAGAGTTTG 
CATAAAGCAT CAGGCAGTGC TGACAAAGGG GGCTCTTTCC TGACTGGCAA AAGAGCTGCC 
GTACTCTTCC AGCAAGTCAA AGAAACAATC AAAGAGAACA TAAAGGAGCA TCTTCTTGAT 
GATGAAGATG AGGACGAGGA GATACTGAGG CAGAAGAGAG AAGAAAGTGA CCCAGAGTAT 
CGATCTAGCA AATCTAAGCC ATTGACTTTA TTAGAGTATA ACCTAACAAT GGATACTGCC 
AAGTTGTTTA TGTCCTGTCT TCATGCCTGG GGTTTGAATG AAGTTCTAGA TGAAGTTTGT 
CTTGACCGCC TTGGAATGCT TAAACCCCAC TGTACAGTCT CCTTTGGTCT TCTGTCAAGA 
GGAGGCCACA TGTCATTGAT GCTTCCTGGT CATAACCAGC CTATATGTAA GCCATCCTGT 
GGAAAACCAG AAGGGGAAAG AAAACTCTCA ACCACAGATG GAGTAGGAAA AGGAACCTAT 
GGAGTATCCC GGGCTGTTAC CACTCAGCAC CTTCTGTCTA TTATATCTTT GGCAAACACC 
TTAATGAGTA TGACCAATGC AACTTTTATT GGTGATCATA TGAAGAAGGG CCCTGTCAGG 
CCACCTAGGC CAAGCACCCC AGACTTTTCC AAAGTGAAGG GTTCCCCTCC AACTTCCAGT 
AATGTTGTTC AAGGACAGAT TAAACAAGCT GCTGCACCTG TCGTTTCCGC TCGGTCTGAT 
ACTGATCACT CTGGTTCTGA CCCTGCTCCT ACTCCTGCTT TACATGCCTG TTTCTTAGTA 
AATGAAGGAT GGAGTCAACT AGCTGCTATG CATTGTGTTA TGCTTCCAGA CCTGTTGGGA 
TTGGATAAAT TTAGACCTCC ACTTCTAGAA ATGCTGGCTC GGAGATGGCA GGATCGATGC 
CTGGAGGTAA GAGAAGCGGC TCAAGCTTTA CTGTTGGCAG AACTGAGAAG AATTGAACAA 
GGAGGAAGAA AAGAAACAAT TGATGCTTGG GCTCCTTATT TACCTCAGTA TATGGATAGT 
GTTATATCAC CTGGAGTGAC AGCAGAATCC ATTCAGACTG CAAATGCTAG TCCAGATGCC 
TTGGGGACTG AAACTAAAGT CCAAGAAGAA GAGCATGATC TTGTTGATGA TGACATCACA 
GCAGGGTGTC TATCATCAGG TCTCCCACAC ATGAAGAAAA TATCCACATC ATATGAAGAA 
AGACGTAAAC AAGCTACAGC TATTGTTTTA TTAGGAGTTA TAGGAGCCGA ATTTGGTGCA 
GAAATTGAAC CTCCGAAATT GTTGACAAGA CCTCGAAGTT CCAGCCAAAT TCCCGAAGGG 
TTTGGTTTGA CCAGTGGTGG ATCCAACTAT TCATTAGCCA GACATACTTG CAAGGCATTG 
ACGTTTCTTC TCCTACAACC TCCTAGTCCT AAACTACCAC CTCACAGCAC AATCAGAAGA 
ACTGCTATTG ATCTAATAGG ACGAGGTTTT ACAGTATGGG AGCCTTATAT GGATGTGTCT 
GCTGTTCTAA TGGGCCTTCT CGAACTCTGT GCTGATGCTG AGAAGCAACT GGCTAACATC 
ACAATGGGGC TGCCCCTGAG TCCAGCAGCT GACTCCGCCC GCTCCGCAAG ACATGCCCTC 
TCACTCATTG CCACTGCCAG ACCACCCGCC TTCATCACAA CTATTGCCAA GGAGGCCAGC 
ATATGCAGTC CTTCATATGC TAAGATGATG TTCCTGAGTA CATCGACATA CTGCTCTAGC 
AGCAAACACC CAGTCACAGC AGAATATTCA TACTACAACT CTTGCTCGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100927741 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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