PHKA1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHKA1
Entrez Gene ID	100923997
Full Name	phosphorylase kinase regulatory subunit alpha 1
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031945175.1	XP_031801035.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
XM_012553445.1	XP_012408899.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
XM_023507123.1	XP_023362891.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
XM_031945176.1	XP_031801036.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2

Pathways from BioSystems
Gene Ontology

The following PHKA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHKA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa137195	XM_012553445.1 Latest version!	Sarcophilus harrisii phosphorylase kinase, alpha 1 (muscle) (PHKA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa158125	XM_023507123.1 Latest version!	Sarcophilus harrisii phosphorylase kinase regulatory subunit alpha 1 (PHKA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa241367	XM_031945175.1 Latest version!	Sarcophilus harrisii phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa241368	XM_031945176.1 Latest version!	Sarcophilus harrisii phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHKA1 ( XM_012553445.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012408899.1 Sarcophilus harrisii phosphorylase kinase, alpha 1 (muscle) (PHKA1), mRNA.

Price & Availability↑

CloneID	OSa137195
Clone ID Related Accession (Same CDS sequence)	XM_012553445.1
Accession Version	XM_012553445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003849631.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCTGGAGC TGTGCTTTGA AGGAAACCAG AGATTCCAGT TTCTAGAGGT GGAGAATCCT 
GTGACTGGCT TACTTCCAGC AAGCTGTGAT CAGAAAGATG CTTGGGTCCG GGATAACGTC 
TACAGCATCC TGGCGGTGTG GGGCCTGGGC CTGGCTTACC GCAAGAATGC CGACCGCGAC 
GAGGACAAGG CCAAAGCCTA TGAGTTGGAG CAGAGTGTCG TGAAGCTGAT GAGGGGGCTG 
CTGCAGTGCA TGATCAGACA GGTGGACAAA GTCGAGTCCT TCAAATACAG TCAGAGCACC 
AAGGATAGTC TCCACGCCAA GTACAACACC AACACCTGCG CCACCGTGGT AGGAGACGAT 
CAATGGGGCC ACTTGCAGCT GGACGCCACC TCCGTGTTCC TGCTCTTCCT GGCCCAAATG 
ACAGCCTCAG GGCTCCACAT CATCCACAGC CTAGATGAGG TCAATTTCAT ACAGAATCTT 
GTGTTTTACA TCGAGTCTGC TTATAAAACA GCTGATTTTG GCATATGGGA ACGTGGCGAT 
AAGACCAACC AAGGCATATC GGAACTGAAC GCGAGCTCTG TCGGGATGGC CAAGGCGGCC 
CTGGAAGCCT TGGATGAGCT GGACCTGTTT GGCATGAAGG GGGGTCCCCA GTCCGTAATT 
CACGTCTTGT CGGATGAGGT CCAGCACTGC CAGTCCATCC TCCATTCCAT GCTGCCCCGG 
GCCTCGATGT CCAAAGAGGT GGACGCCAGT GTCCTTTCGG TGATTTCCTT CCCAGCATTC 
GCGGTAGAGG ACAGTCAGCT GGTGGAGATT ACGAAGCAAG AAATAGTCAC CAAGCTTCAG 
GGTCGTTATG GCTGCTGTCG CTTTCTCCGA GATGGGTACA AAACCCCCAA AGAGGATCCT 
CATCGTCTGT ACTATGAGCC AGCTGAGCTG AAGCTGTTTG AGAACATTGA GTGTGAATGG 
CCTTTGTTCT GGACCTACTT CATCCTGGAC GGGGTCTTCA GTGGCAACGT GGAGCAGGTC 
CAGGAGTACC GGGAAGCCCT TGAAGGGGTC CTTATCAAGG GCAAGAATGG GGTTCGCCTT 
CTCCCTGAGC TCTATAGTGT CCCTCCTGAA AAGGTCGACG AAGAGTACCA GCATCCTCAC 
ACCGTGGACC GAGTGCCCAT GGGCAAGCTG CCCCACATGT GGGGCCAGTC CCTATACATC 
CTGGGAAGCT TGATGGCCGA GGGGTTTTTA GCACCTGGAG AAATTGATCC TCTGAATCGC 
AGGTTTTCAA CTGTCCCCAA GCCAGATGTT GTGGTTCAAG TGTCCTTTGT AGCTGAGACA 
GAAGACATCA AGACCATCTT GAGGAACAAT GGAATTGATG TGGAGACCAT TGCCGAAGTG 
TACCCCATCA GAGTACAACC AGCCCGAATC CTCAGCCATA TCTATGCCAG CCTAGGCCGC 
AACAACCGGA TGAAACTCAG TGGAAGACCG TATCGGCACA TGGGGGTCCT AGGAACATCT 
AAACTCTATG ACATTCGTAA AACCATCTTT GCTTTCACAC CACAGTTCAT AGATCAGCAA 
CAGTTCTATT TGGCCCTTGA CAACAAGATG ATAGTAGAGA TGCTGCGAAC AGACCTGTCC 
TACCTGTGTA GCCGCTGGAG GATGACAGGC CAGCCCACCA TCACCTTTCC CATCCTGCAC 
ACCATGCTTG ATGAATCGGG AACTGCCATA AATGCATCTG TCTTGACTAT GCTCAGAAAA 
ATGCAGGATG GCTATTTCGG CGGGGCCAGG GTCCAAGCTG GTAAGCTGTC TGAGTTCCTG 
ACGACATCCT GCTGTACACG CCTGAGCTTC ATGGATCCTG GCCAGGAGGG TAAGCTGTAT 
GGTGGGAATT GTGATGATGA TGAAGAAGAT GACTATAATG ATGAAGAGCT CTACTCGGAC 
AACTGGCTGG GTGGCTTTGA CTCCCGCAAG CAAGTGGGCG ACAGTGATGA AGTTATGGGG 
TACATAGACC ACCTTTTGGA ACACACTGCG CCCAAGCCCA CACGGGCCCC CGCCGCCCAG 
CAAGGAGGGC TCAGTCGCTT CCGAACGGCA GTACAAACAA CCCATGACTA CATGTCCTTG 
GTGACCAAGG CTAAGGAGCT GCATGTACAG AATGCTCACA TGTACCTTCC CACAAAGTTA 
CTGCCAGCCT CCCGAACTTC CTTCAGCTTC CTGGATGCCA CGCCTCTGTC TCCCCAAGCT 
GAGCAGGTTC CTTCTGTCCA CGTGGAGATG CACCTTCCCC GGGACGAATC TGGGGAGATA 
GACTTCCAGG CCCTGGTGGG CCAGTTGCAC GCGTGCCCAA GCTTACAGGA CCAAGCTGAC 
ATCCTCTACG TGCTTTACAC CATGAAGGGT CCTGACTGGA ACACTTCCAT AGCTGGCGGT 
GAGGAAAGTG GGACGGTCCA GGAGCTCCTC GTTGCCCTGT ACGGCAAAGC CGGGGAAGCA 
CGCCATTGGG GCCTGATCCG CTACATCTCT GGCATTCTGA AGAAGAAAGT GGAAGCCCTG 
GATGAGGCCT GCACGGATCT GCTGTCTCAC CAGAAGCACC TGACGGTGGG ACTCCCTCCA 
GAGCCACGGG AGAAGACCAT CAATGCTCCC CTACCCTATG AAGAGCTCAC CAAGCTGATC 
GATGAAGCAA GTGAGAACGA TGTAAACATC TCCATCCTCA CCCAGGAAAT CCTGGTGTAC 
CTGGCCATGT ACATGCGAAC TCAACCCAGC CTTTTTGCGG AGATGTTCCG GCTCCGGATC 
GGTCTCATCA TCCAGGTGAT GGCGACGGAA CTGGTGCAGT CGCTTAACTG CTCAGCTCAA 
GAAGCCACAG AGAGCCTGAT GAACCTCAGT CCTTCGGCCA TGAAGAACCT ATTGCACCAC 
ATTCTCAGTG GCAAAGAGTT TGGCGTGGAA AGAAGTGTGC GTCCTACCGA TTCCAATGTC 
AGCCCCGCTA TTTCCATTCA TGAAGTCGGC GCTGTGGGAG TGAGCCGAAC CGAACGGGCC 
GGAATCATGC AGTTAAAAAG TGACATAAAG CAGGTGGAAT TACGCCGGCA TTCTATCTCA 
GTTGAGAATC AGTCTCCCGG ACCTTCATTA AGCCCAAGTG GGAGCTCCTT CACAGGCACC 
TTCAGAGAGC AGGCCTGGAA AGACTCTCGC CAGGGCCAGT GGCAGCGCCG GCGCAGGCTC 
GATGGAGCCC TGAACAGGGT CCCCATCGGG TTTTATCAGA AAGTGTGGAA AGTCCTGCAG 
AAGTGCCACG GTCTCTCCGT GGAAGGCTTC GTTCTCCCTT CTTCGACGAC CAGAGAGATG 
ACACCAGGGG AGATCAAGTT CTCGGTTCAC GTGGAGTCCG TCCTCAACCG TGTTCCTCAG 
CCAGAATACC GGCAGCTCCT GGTTGAAGCC ATCCTCGTCC TGACCATGCT GGCAGACATT 
GAAATCCAGA GCGTAGGTGG CATCATCGCT GTGGAAAAGA TCGTGCACAT CGCCAACGAG 
CTCTTCCTCC AAGAGCAGAA AGCGCTCGGG GCAGACGACA GCATGCTGGC CAAGGATCCG 
TCGTCTGGCA TCTGTACCCT CCTGTACGAC AGCGCCCCCA GTGGCAGGGT CGGCACCATG 
ACCTACCTCT CGAAAGCTGC AGCTACCTAC GTGCAAGAGT TCCTGCCTCA CAGCGTCTGC 
GCCATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012408899.1
CDS	1..3672
Translation	MLELCFEGNQRFQFLEVENPVTGLLPASCDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMIRQVDKVESFKYSQSTKDSLHAKYNTNTCATVVGDDQWGHLQLDATSVFLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIESAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGMKGGPQSVIHVLSDEVQHCQSILHSMLPRASMSKEVDASVLSVISFPAFAVEDSQLVEITKQEIVTKLQGRYGCCRFLRDGYKTPKEDPHRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNVEQVQEYREALEGVLIKGKNGVRLLPELYSVPPEKVDEEYQHPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSFVAETEDIKTILRNNGIDVETIAEVYPIRVQPARILSHIYASLGRNNRMKLSGRPYRHMGVLGTSKLYDIRKTIFAFTPQFIDQQQFYLALDNKMIVEMLRTDLSYLCSRWRMTGQPTITFPILHTMLDESGTAINASVLTMLRKMQDGYFGGARVQAGKLSEFLTTSCCTRLSFMDPGQEGKLYGGNCDDDEEDDYNDEELYSDNWLGGFDSRKQVGDSDEVMGYIDHLLEHTAPKPTRAPAAQQGGLSRFRTAVQTTHDYMSLVTKAKELHVQNAHMYLPTKLLPASRTSFSFLDATPLSPQAEQVPSVHVEMHLPRDESGEIDFQALVGQLHACPSLQDQADILYVLYTMKGPDWNTSIAGGEESGTVQELLVALYGKAGEARHWGLIRYISGILKKKVEALDEACTDLLSHQKHLTVGLPPEPREKTINAPLPYEELTKLIDEASENDVNISILTQEILVYLAMYMRTQPSLFAEMFRLRIGLIIQVMATELVQSLNCSAQEATESLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEVGAVGVSRTERAGIMQLKSDIKQVELRRHSISVENQSPGPSLSPSGSSFTGTFREQAWKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIQSVGGIIAVEKIVHIANELFLQEQKALGADDSMLAKDPSSGICTLLYDSAPSGRVGTMTYLSKAAATYVQEFLPHSVCAMQ

Target ORF information:

RefSeq Version	XM_012553445.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii phosphorylase kinase, alpha 1 (muscle) (PHKA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012553445.1

ORF Insert Sequence:

ATGCTGGAGC TGTGCTTTGA AGGAAACCAG AGATTCCAGT TTCTAGAGGT GGAGAATCCT 
GTGACTGGCT TACTTCCAGC AAGCTGTGAT CAGAAAGATG CTTGGGTCCG GGATAACGTC 
TACAGCATCC TGGCGGTGTG GGGCCTGGGC CTGGCTTACC GCAAGAATGC CGACCGCGAC 
GAGGACAAGG CCAAAGCCTA TGAGTTGGAG CAGAGTGTCG TGAAGCTGAT GAGGGGGCTG 
CTGCAGTGCA TGATCAGACA GGTGGACAAA GTCGAGTCCT TCAAATACAG TCAGAGCACC 
AAGGATAGTC TCCACGCCAA GTACAACACC AACACCTGCG CCACCGTGGT AGGAGACGAT 
CAATGGGGCC ACTTGCAGCT GGACGCCACC TCCGTGTTCC TGCTCTTCCT GGCCCAAATG 
ACAGCCTCAG GGCTCCACAT CATCCACAGC CTAGATGAGG TCAATTTCAT ACAGAATCTT 
GTGTTTTACA TCGAGTCTGC TTATAAAACA GCTGATTTTG GCATATGGGA ACGTGGCGAT 
AAGACCAACC AAGGCATATC GGAACTGAAC GCGAGCTCTG TCGGGATGGC CAAGGCGGCC 
CTGGAAGCCT TGGATGAGCT GGACCTGTTT GGCATGAAGG GGGGTCCCCA GTCCGTAATT 
CACGTCTTGT CGGATGAGGT CCAGCACTGC CAGTCCATCC TCCATTCCAT GCTGCCCCGG 
GCCTCGATGT CCAAAGAGGT GGACGCCAGT GTCCTTTCGG TGATTTCCTT CCCAGCATTC 
GCGGTAGAGG ACAGTCAGCT GGTGGAGATT ACGAAGCAAG AAATAGTCAC CAAGCTTCAG 
GGTCGTTATG GCTGCTGTCG CTTTCTCCGA GATGGGTACA AAACCCCCAA AGAGGATCCT 
CATCGTCTGT ACTATGAGCC AGCTGAGCTG AAGCTGTTTG AGAACATTGA GTGTGAATGG 
CCTTTGTTCT GGACCTACTT CATCCTGGAC GGGGTCTTCA GTGGCAACGT GGAGCAGGTC 
CAGGAGTACC GGGAAGCCCT TGAAGGGGTC CTTATCAAGG GCAAGAATGG GGTTCGCCTT 
CTCCCTGAGC TCTATAGTGT CCCTCCTGAA AAGGTCGACG AAGAGTACCA GCATCCTCAC 
ACCGTGGACC GAGTGCCCAT GGGCAAGCTG CCCCACATGT GGGGCCAGTC CCTATACATC 
CTGGGAAGCT TGATGGCCGA GGGGTTTTTA GCACCTGGAG AAATTGATCC TCTGAATCGC 
AGGTTTTCAA CTGTCCCCAA GCCAGATGTT GTGGTTCAAG TGTCCTTTGT AGCTGAGACA 
GAAGACATCA AGACCATCTT GAGGAACAAT GGAATTGATG TGGAGACCAT TGCCGAAGTG 
TACCCCATCA GAGTACAACC AGCCCGAATC CTCAGCCATA TCTATGCCAG CCTAGGCCGC 
AACAACCGGA TGAAACTCAG TGGAAGACCG TATCGGCACA TGGGGGTCCT AGGAACATCT 
AAACTCTATG ACATTCGTAA AACCATCTTT GCTTTCACAC CACAGTTCAT AGATCAGCAA 
CAGTTCTATT TGGCCCTTGA CAACAAGATG ATAGTAGAGA TGCTGCGAAC AGACCTGTCC 
TACCTGTGTA GCCGCTGGAG GATGACAGGC CAGCCCACCA TCACCTTTCC CATCCTGCAC 
ACCATGCTTG ATGAATCGGG AACTGCCATA AATGCATCTG TCTTGACTAT GCTCAGAAAA 
ATGCAGGATG GCTATTTCGG CGGGGCCAGG GTCCAAGCTG GTAAGCTGTC TGAGTTCCTG 
ACGACATCCT GCTGTACACG CCTGAGCTTC ATGGATCCTG GCCAGGAGGG TAAGCTGTAT 
GGTGGGAATT GTGATGATGA TGAAGAAGAT GACTATAATG ATGAAGAGCT CTACTCGGAC 
AACTGGCTGG GTGGCTTTGA CTCCCGCAAG CAAGTGGGCG ACAGTGATGA AGTTATGGGG 
TACATAGACC ACCTTTTGGA ACACACTGCG CCCAAGCCCA CACGGGCCCC CGCCGCCCAG 
CAAGGAGGGC TCAGTCGCTT CCGAACGGCA GTACAAACAA CCCATGACTA CATGTCCTTG 
GTGACCAAGG CTAAGGAGCT GCATGTACAG AATGCTCACA TGTACCTTCC CACAAAGTTA 
CTGCCAGCCT CCCGAACTTC CTTCAGCTTC CTGGATGCCA CGCCTCTGTC TCCCCAAGCT 
GAGCAGGTTC CTTCTGTCCA CGTGGAGATG CACCTTCCCC GGGACGAATC TGGGGAGATA 
GACTTCCAGG CCCTGGTGGG CCAGTTGCAC GCGTGCCCAA GCTTACAGGA CCAAGCTGAC 
ATCCTCTACG TGCTTTACAC CATGAAGGGT CCTGACTGGA ACACTTCCAT AGCTGGCGGT 
GAGGAAAGTG GGACGGTCCA GGAGCTCCTC GTTGCCCTGT ACGGCAAAGC CGGGGAAGCA 
CGCCATTGGG GCCTGATCCG CTACATCTCT GGCATTCTGA AGAAGAAAGT GGAAGCCCTG 
GATGAGGCCT GCACGGATCT GCTGTCTCAC CAGAAGCACC TGACGGTGGG ACTCCCTCCA 
GAGCCACGGG AGAAGACCAT CAATGCTCCC CTACCCTATG AAGAGCTCAC CAAGCTGATC 
GATGAAGCAA GTGAGAACGA TGTAAACATC TCCATCCTCA CCCAGGAAAT CCTGGTGTAC 
CTGGCCATGT ACATGCGAAC TCAACCCAGC CTTTTTGCGG AGATGTTCCG GCTCCGGATC 
GGTCTCATCA TCCAGGTGAT GGCGACGGAA CTGGTGCAGT CGCTTAACTG CTCAGCTCAA 
GAAGCCACAG AGAGCCTGAT GAACCTCAGT CCTTCGGCCA TGAAGAACCT ATTGCACCAC 
ATTCTCAGTG GCAAAGAGTT TGGCGTGGAA AGAAGTGTGC GTCCTACCGA TTCCAATGTC 
AGCCCCGCTA TTTCCATTCA TGAAGTCGGC GCTGTGGGAG TGAGCCGAAC CGAACGGGCC 
GGAATCATGC AGTTAAAAAG TGACATAAAG CAGGTGGAAT TACGCCGGCA TTCTATCTCA 
GTTGAGAATC AGTCTCCCGG ACCTTCATTA AGCCCAAGTG GGAGCTCCTT CACAGGCACC 
TTCAGAGAGC AGGCCTGGAA AGACTCTCGC CAGGGCCAGT GGCAGCGCCG GCGCAGGCTC 
GATGGAGCCC TGAACAGGGT CCCCATCGGG TTTTATCAGA AAGTGTGGAA AGTCCTGCAG 
AAGTGCCACG GTCTCTCCGT GGAAGGCTTC GTTCTCCCTT CTTCGACGAC CAGAGAGATG 
ACACCAGGGG AGATCAAGTT CTCGGTTCAC GTGGAGTCCG TCCTCAACCG TGTTCCTCAG 
CCAGAATACC GGCAGCTCCT GGTTGAAGCC ATCCTCGTCC TGACCATGCT GGCAGACATT 
GAAATCCAGA GCGTAGGTGG CATCATCGCT GTGGAAAAGA TCGTGCACAT CGCCAACGAG 
CTCTTCCTCC AAGAGCAGAA AGCGCTCGGG GCAGACGACA GCATGCTGGC CAAGGATCCG 
TCGTCTGGCA TCTGTACCCT CCTGTACGAC AGCGCCCCCA GTGGCAGGGT CGGCACCATG 
ACCTACCTCT CGAAAGCTGC AGCTACCTAC GTGCAAGAGT TCCTGCCTCA CAGCGTCTGC 
GCCATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_023507123.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023362891.1 Sarcophilus harrisii phosphorylase kinase regulatory subunit alpha 1 (PHKA1), mRNA.

Price & Availability↑

CloneID	OSa158125
Clone ID Related Accession (Same CDS sequence)	XM_023507123.1
Accession Version	XM_023507123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003849631.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGCTGGAGC TGTGCTTTGA AGGAAACCAG AGATTCCAGT TTCTAGAGGT GGAGAATCCT 
GTGACTGGCT TACTTCCAGC AAGCTGTGAT CAGAAAGATG CTTGGGTCCG GGATAACGTC 
TACAGCATCC TGGCGGTGTG GGGCCTGGGC CTGGCTTACC GCAAGAATGC CGACCGCGAC 
GAGGACAAGG CCAAAGCCTA TGAGTTGGAG CAGAGTGTCG TGAAGCTGAT GAGGGGGCTG 
CTGCAGTGCA TGATCAGACA GGTGGACAAA GTCGAGTCCT TCAAATACAG TCAGAGCACC 
AAGGATAGTC TCCACGCCAA GTACAACACC AACACCTGCG CCACCGTGGT AGGAGACGAT 
CAATGGGGCC ACTTGCAGCT GGACGCCACC TCCGTGTTCC TGCTCTTCCT GGCCCAAATG 
ACAGCCTCAG GGCTCCACAT CATCCACAGC CTAGATGAGG TCAATTTCAT ACAGAATCTT 
GTGTTTTACA TCGAGTCTGC TTATAAAACA GCTGATTTTG GCATATGGGA ACGTGGCGAT 
AAGACCAACC AAGGCATATC GGAACTGAAC GCGAGCTCTG TCGGGATGGC CAAGGCGGCC 
CTGGAAGCCT TGGATGAGCT GGACCTGTTT GGCATGAAGG GGGGTCCCCA GTCCGTAATT 
CACGTCTTGT CGGATGAGGT CCAGCACTGC CAGTCCATCC TCCATTCCAT GCTGCCCCGG 
GCCTCGATGT CCAAAGAGGT GGACGCCAGT GTCCTTTCGG TGATTTCCTT CCCAGCATTC 
GCGGTAGAGG ACAGTCAGCT GGTGGAGATT ACGAAGCAAG AAATAGTCAC CAAGCTTCAG 
GGTCGTTATG GCTGCTGTCG CTTTCTCCGA GATGGGTACA AAACCCCCAA AGAGGATCCT 
CATCGTCTGT ACTATGAGCC AGCTGAGCTG AAGCTGTTTG AGAACATTGA GTGTGAATGG 
CCTTTGTTCT GGACCTACTT CATCCTGGAC GGGGTCTTCA GTGGCAACGT GGAGCAGGTC 
CAGGAGTACC GGGAAGCCCT TGAAGGGGTC CTTATCAAGG GCAAGAATGG GGTTCGCCTT 
CTCCCTGAGC TCTATAGTGT CCCTCCTGAA AAGGTCGACG AAGAGTACCA GCATCCTCAC 
ACCGTGGACC GAGTGCCCAT GGGCAAGCTG CCCCACATGT GGGGCCAGTC CCTATACATC 
CTGGGAAGCT TGATGGCCGA GGGGTTTTTA GCACCTGGAG AAATTGATCC TCTGAATCGC 
AGGTTTTCAA CTGTCCCCAA GCCAGATGTT GTGGTTCAAG TGTCCTTTGT AGCTGAGACA 
GAAGACATCA AGACCATCTT GAGGAACAAT GGAATTGATG TGGAGACCAT TGCCGAAGTG 
TACCCCATCA GAGTACAACC AGCCCGAATC CTCAGCCATA TCTATGCCAG CCTAGGCCGC 
AACAACCGGA TGAAACTCAG TGGAAGACCG TATCGGCACA TGGGGGTCCT AGGAACATCT 
AAACTCTATG ACATTCGTAA AACCATCTTT GCTTTCACAC CACAGTTCAT AGATCAGCAA 
CAGTTCTATT TGGCCCTTGA CAACAAGATG ATAGTAGAGA TGCTGCGAAC AGACCTGTCC 
TACCTGTGTA GCCGCTGGAG GATGACAGGC CAGCCCACCA TCACCTTTCC CATCCTGCAC 
ACCATGCTTG ATGAATCGGG AACTGCCATA AATGCATCTG TCTTGACTAT GCTCAGAAAA 
ATGCAGGATG GCTATTTCGG CGGGGCCAGG GTCCAAGCTG GTAAGCTGTC TGAGTTCCTG 
ACGACATCCT GCTGTACACG CCTGAGCTTC ATGGATCCTG GCCAGGAGGG TAAGCTGTAT 
GGTGGGAATT GTGATGATGA TGAAGAAGAT GACTATAATG ATGAAGAGCT CTACTCGGAC 
AACTGGCTGG GTGGCTTTGA CTCCCGCAAG CAAGTGGGCG ACAGTGATGA AGTTATGGGG 
TACATAGACC ACCTTTTGGA ACACACTGCG CCCAAGCCCA CACGGGCCCC CGCCGCCCAG 
CAAGGAGGGC TCAGTCGCTT CCGAACGGCA GTACAAACAA CCCATGACTA CATGTCCTTG 
GTGACCAAGG CTAAGGAGCT GCATGTACAG AATGCTCACA TGTACCTTCC CACAAAGTTA 
CTGCCAGCCT CCCGAACTTC CTTCAGCTTC CTGGATGCCA CGCCTCTGTC TCCCCAAGCT 
GAGCAGGTTC CTTCTGTCCA CGTGGAGATG CACCTTCCCC GGGACGAATC TGGGGAGATA 
GACTTCCAGG CCCTGGTGGG CCAGTTGCAC GCGTGCCCAA GCTTACAGGA CCAAGCTGAC 
ATCCTCTACG TGCTTTACAC CATGAAGGGT CCTGACTGGA ACACTTCCAT AGCTGGCGGT 
GAGGAAAGTG GGACGGTCCA GGAGCTCCTC GTTGCCCTGT ACGGCAAAGC CGGGGAAGCA 
CGCCATTGGG GCCTGATCCG CTACATCTCT GGCATTCTGA AGAAGAAAGT GGAAGCCCTG 
GATGAGGCCT GCACGGATCT GCTGTCTCAC CAGAAGCACC TGACGGTGGG ACTCCCTCCA 
GAGCCACGGG AGAAGACCAT CAATGCTCCC CTACCCTATG AAGAGCTCAC CAAGCTGATC 
GATGAAGCAA GTGAGAACGA TGTAAACATC TCCATCCTCA CCCAGGAAAT CCTGGTGTAC 
CTGGCCATGT ACATGCGAAC TCAACCCAGC CTTTTTGCGG AGATGTTCCG GCTCCGGATC 
GGTCTCATCA TCCAGGTGAT GGCGACGGAA CTGGTGCAGT CGCTTAACTG CTCAGCTCAA 
GAAGCCACAG AGAGCCTGAT GAACCTCAGT CCTTCGGCCA TGAAGAACCT ATTGCACCAC 
ATTCTCAGTG GCAAAGAGTT TGGCGTGGAA AGAAGTGTGC GTCCTACCGA TTCCAATGTC 
AGCCCCGCTA TTTCCATTCA TGAAGTCGGC GCTGTGGGAG TGAGCCGAAC CGAACGGGCC 
GGAATCATGC AGTTAAAAAG TGACATAAAG CAGGTGGAAT TACGCCGGCA TTCTATCTCA 
GTTGAGAATC AGCCCAGTGG TGGCCATGGC CTGAGTATTG AGCCAACATC CTCGCCTTTT 
CTGTCTCCCG GACCTTCATT AAGCCCAAGT GGGAGCTCCT TCACAGGCAC CTTCAGAGAG 
CAGGCCTGGA AAGACTCTCG CCAGGGCCAG TGGCAGCGCC GGCGCAGGCT CGATGGAGCC 
CTGAACAGGG TCCCCATCGG GTTTTATCAG AAAGTGTGGA AAGTCCTGCA GAAGTGCCAC 
GGTCTCTCCG TGGAAGGCTT CGTTCTCCCT TCTTCGACGA CCAGAGAGAT GACACCAGGG 
GAGATCAAGT TCTCGGTTCA CGTGGAGTCC GTCCTCAACC GTGTTCCTCA GCCAGAATAC 
CGGCAGCTCC TGGTTGAAGC CATCCTCGTC CTGACCATGC TGGCAGACAT TGAAATCCAG 
AGCGTAGGTG GCATCATCGC TGTGGAAAAG ATCGTGCACA TCGCCAACGA GCTCTTCCTC 
CAAGAGCAGA AAGCGCTCGG GGCAGACGAC AGCATGCTGG CCAAGGATCC GTCGTCTGGC 
ATCTGTACCC TCCTGTACGA CAGCGCCCCC AGTGGCAGGT TCGGCACCAT GACCTACCTC 
AAAGCGCTCG GGGCAGACGA CAGCATGCTG GCCAAGGATC CGTCGTCTGG CATCTGTACC 
CTCCTGTACG ACAGCGCCCC CAGTGGCAGG GTCGGCACCA TGACCTACCT CTCGAAAGCT 
GCAGCTACCT ACGTGCAAGA GTTCCTGCCT CACAGCGTCT GCGCCATGCA GCACTTAGAG 
GGTGTGGGAC ACATCTGGCT GCAGCTGAGT GTGCACATGG ATGGGACTCT CACTGTGCCT 
GTGTGGGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023362891.1
CDS	1..3912
Translation	MLELCFEGNQRFQFLEVENPVTGLLPASCDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMIRQVDKVESFKYSQSTKDSLHAKYNTNTCATVVGDDQWGHLQLDATSVFLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIESAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGMKGGPQSVIHVLSDEVQHCQSILHSMLPRASMSKEVDASVLSVISFPAFAVEDSQLVEITKQEIVTKLQGRYGCCRFLRDGYKTPKEDPHRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNVEQVQEYREALEGVLIKGKNGVRLLPELYSVPPEKVDEEYQHPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSFVAETEDIKTILRNNGIDVETIAEVYPIRVQPARILSHIYASLGRNNRMKLSGRPYRHMGVLGTSKLYDIRKTIFAFTPQFIDQQQFYLALDNKMIVEMLRTDLSYLCSRWRMTGQPTITFPILHTMLDESGTAINASVLTMLRKMQDGYFGGARVQAGKLSEFLTTSCCTRLSFMDPGQEGKLYGGNCDDDEEDDYNDEELYSDNWLGGFDSRKQVGDSDEVMGYIDHLLEHTAPKPTRAPAAQQGGLSRFRTAVQTTHDYMSLVTKAKELHVQNAHMYLPTKLLPASRTSFSFLDATPLSPQAEQVPSVHVEMHLPRDESGEIDFQALVGQLHACPSLQDQADILYVLYTMKGPDWNTSIAGGEESGTVQELLVALYGKAGEARHWGLIRYISGILKKKVEALDEACTDLLSHQKHLTVGLPPEPREKTINAPLPYEELTKLIDEASENDVNISILTQEILVYLAMYMRTQPSLFAEMFRLRIGLIIQVMATELVQSLNCSAQEATESLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEVGAVGVSRTERAGIMQLKSDIKQVELRRHSISVENQPSGGHGLSIEPTSSPFLSPGPSLSPSGSSFTGTFREQAWKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIQSVGGIIAVEKIVHIANELFLQEQKALGADDSMLAKDPSSGICTLLYDSAPSGRFGTMTYLKALGADDSMLAKDPSSGICTLLYDSAPSGRVGTMTYLSKAAATYVQEFLPHSVCAMQHLEGVGHIWLQLSVHMDGTLTVPVWA

Target ORF information:

RefSeq Version	XM_023507123.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii phosphorylase kinase regulatory subunit alpha 1 (PHKA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023507123.1

ORF Insert Sequence:

ATGCTGGAGC TGTGCTTTGA AGGAAACCAG AGATTCCAGT TTCTAGAGGT GGAGAATCCT 
GTGACTGGCT TACTTCCAGC AAGCTGTGAT CAGAAAGATG CTTGGGTCCG GGATAACGTC 
TACAGCATCC TGGCGGTGTG GGGCCTGGGC CTGGCTTACC GCAAGAATGC CGACCGCGAC 
GAGGACAAGG CCAAAGCCTA TGAGTTGGAG CAGAGTGTCG TGAAGCTGAT GAGGGGGCTG 
CTGCAGTGCA TGATCAGACA GGTGGACAAA GTCGAGTCCT TCAAATACAG TCAGAGCACC 
AAGGATAGTC TCCACGCCAA GTACAACACC AACACCTGCG CCACCGTGGT AGGAGACGAT 
CAATGGGGCC ACTTGCAGCT GGACGCCACC TCCGTGTTCC TGCTCTTCCT GGCCCAAATG 
ACAGCCTCAG GGCTCCACAT CATCCACAGC CTAGATGAGG TCAATTTCAT ACAGAATCTT 
GTGTTTTACA TCGAGTCTGC TTATAAAACA GCTGATTTTG GCATATGGGA ACGTGGCGAT 
AAGACCAACC AAGGCATATC GGAACTGAAC GCGAGCTCTG TCGGGATGGC CAAGGCGGCC 
CTGGAAGCCT TGGATGAGCT GGACCTGTTT GGCATGAAGG GGGGTCCCCA GTCCGTAATT 
CACGTCTTGT CGGATGAGGT CCAGCACTGC CAGTCCATCC TCCATTCCAT GCTGCCCCGG 
GCCTCGATGT CCAAAGAGGT GGACGCCAGT GTCCTTTCGG TGATTTCCTT CCCAGCATTC 
GCGGTAGAGG ACAGTCAGCT GGTGGAGATT ACGAAGCAAG AAATAGTCAC CAAGCTTCAG 
GGTCGTTATG GCTGCTGTCG CTTTCTCCGA GATGGGTACA AAACCCCCAA AGAGGATCCT 
CATCGTCTGT ACTATGAGCC AGCTGAGCTG AAGCTGTTTG AGAACATTGA GTGTGAATGG 
CCTTTGTTCT GGACCTACTT CATCCTGGAC GGGGTCTTCA GTGGCAACGT GGAGCAGGTC 
CAGGAGTACC GGGAAGCCCT TGAAGGGGTC CTTATCAAGG GCAAGAATGG GGTTCGCCTT 
CTCCCTGAGC TCTATAGTGT CCCTCCTGAA AAGGTCGACG AAGAGTACCA GCATCCTCAC 
ACCGTGGACC GAGTGCCCAT GGGCAAGCTG CCCCACATGT GGGGCCAGTC CCTATACATC 
CTGGGAAGCT TGATGGCCGA GGGGTTTTTA GCACCTGGAG AAATTGATCC TCTGAATCGC 
AGGTTTTCAA CTGTCCCCAA GCCAGATGTT GTGGTTCAAG TGTCCTTTGT AGCTGAGACA 
GAAGACATCA AGACCATCTT GAGGAACAAT GGAATTGATG TGGAGACCAT TGCCGAAGTG 
TACCCCATCA GAGTACAACC AGCCCGAATC CTCAGCCATA TCTATGCCAG CCTAGGCCGC 
AACAACCGGA TGAAACTCAG TGGAAGACCG TATCGGCACA TGGGGGTCCT AGGAACATCT 
AAACTCTATG ACATTCGTAA AACCATCTTT GCTTTCACAC CACAGTTCAT AGATCAGCAA 
CAGTTCTATT TGGCCCTTGA CAACAAGATG ATAGTAGAGA TGCTGCGAAC AGACCTGTCC 
TACCTGTGTA GCCGCTGGAG GATGACAGGC CAGCCCACCA TCACCTTTCC CATCCTGCAC 
ACCATGCTTG ATGAATCGGG AACTGCCATA AATGCATCTG TCTTGACTAT GCTCAGAAAA 
ATGCAGGATG GCTATTTCGG CGGGGCCAGG GTCCAAGCTG GTAAGCTGTC TGAGTTCCTG 
ACGACATCCT GCTGTACACG CCTGAGCTTC ATGGATCCTG GCCAGGAGGG TAAGCTGTAT 
GGTGGGAATT GTGATGATGA TGAAGAAGAT GACTATAATG ATGAAGAGCT CTACTCGGAC 
AACTGGCTGG GTGGCTTTGA CTCCCGCAAG CAAGTGGGCG ACAGTGATGA AGTTATGGGG 
TACATAGACC ACCTTTTGGA ACACACTGCG CCCAAGCCCA CACGGGCCCC CGCCGCCCAG 
CAAGGAGGGC TCAGTCGCTT CCGAACGGCA GTACAAACAA CCCATGACTA CATGTCCTTG 
GTGACCAAGG CTAAGGAGCT GCATGTACAG AATGCTCACA TGTACCTTCC CACAAAGTTA 
CTGCCAGCCT CCCGAACTTC CTTCAGCTTC CTGGATGCCA CGCCTCTGTC TCCCCAAGCT 
GAGCAGGTTC CTTCTGTCCA CGTGGAGATG CACCTTCCCC GGGACGAATC TGGGGAGATA 
GACTTCCAGG CCCTGGTGGG CCAGTTGCAC GCGTGCCCAA GCTTACAGGA CCAAGCTGAC 
ATCCTCTACG TGCTTTACAC CATGAAGGGT CCTGACTGGA ACACTTCCAT AGCTGGCGGT 
GAGGAAAGTG GGACGGTCCA GGAGCTCCTC GTTGCCCTGT ACGGCAAAGC CGGGGAAGCA 
CGCCATTGGG GCCTGATCCG CTACATCTCT GGCATTCTGA AGAAGAAAGT GGAAGCCCTG 
GATGAGGCCT GCACGGATCT GCTGTCTCAC CAGAAGCACC TGACGGTGGG ACTCCCTCCA 
GAGCCACGGG AGAAGACCAT CAATGCTCCC CTACCCTATG AAGAGCTCAC CAAGCTGATC 
GATGAAGCAA GTGAGAACGA TGTAAACATC TCCATCCTCA CCCAGGAAAT CCTGGTGTAC 
CTGGCCATGT ACATGCGAAC TCAACCCAGC CTTTTTGCGG AGATGTTCCG GCTCCGGATC 
GGTCTCATCA TCCAGGTGAT GGCGACGGAA CTGGTGCAGT CGCTTAACTG CTCAGCTCAA 
GAAGCCACAG AGAGCCTGAT GAACCTCAGT CCTTCGGCCA TGAAGAACCT ATTGCACCAC 
ATTCTCAGTG GCAAAGAGTT TGGCGTGGAA AGAAGTGTGC GTCCTACCGA TTCCAATGTC 
AGCCCCGCTA TTTCCATTCA TGAAGTCGGC GCTGTGGGAG TGAGCCGAAC CGAACGGGCC 
GGAATCATGC AGTTAAAAAG TGACATAAAG CAGGTGGAAT TACGCCGGCA TTCTATCTCA 
GTTGAGAATC AGCCCAGTGG TGGCCATGGC CTGAGTATTG AGCCAACATC CTCGCCTTTT 
CTGTCTCCCG GACCTTCATT AAGCCCAAGT GGGAGCTCCT TCACAGGCAC CTTCAGAGAG 
CAGGCCTGGA AAGACTCTCG CCAGGGCCAG TGGCAGCGCC GGCGCAGGCT CGATGGAGCC 
CTGAACAGGG TCCCCATCGG GTTTTATCAG AAAGTGTGGA AAGTCCTGCA GAAGTGCCAC 
GGTCTCTCCG TGGAAGGCTT CGTTCTCCCT TCTTCGACGA CCAGAGAGAT GACACCAGGG 
GAGATCAAGT TCTCGGTTCA CGTGGAGTCC GTCCTCAACC GTGTTCCTCA GCCAGAATAC 
CGGCAGCTCC TGGTTGAAGC CATCCTCGTC CTGACCATGC TGGCAGACAT TGAAATCCAG 
AGCGTAGGTG GCATCATCGC TGTGGAAAAG ATCGTGCACA TCGCCAACGA GCTCTTCCTC 
CAAGAGCAGA AAGCGCTCGG GGCAGACGAC AGCATGCTGG CCAAGGATCC GTCGTCTGGC 
ATCTGTACCC TCCTGTACGA CAGCGCCCCC AGTGGCAGGT TCGGCACCAT GACCTACCTC 
AAAGCGCTCG GGGCAGACGA CAGCATGCTG GCCAAGGATC CGTCGTCTGG CATCTGTACC 
CTCCTGTACG ACAGCGCCCC CAGTGGCAGG GTCGGCACCA TGACCTACCT CTCGAAAGCT 
GCAGCTACCT ACGTGCAAGA GTTCCTGCCT CACAGCGTCT GCGCCATGCA GCACTTAGAG 
GGTGTGGGAC ACATCTGGCT GCAGCTGAGT GTGCACATGG ATGGGACTCT CACTGTGCCT 
GTGTGGGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_031945175.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031801035.1 Sarcophilus harrisii phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X1, mRNA.

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CloneID	OSa241367
Clone ID Related Accession (Same CDS sequence)	XM_031945175.1
Accession Version	XM_031945175.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045432.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GGAGCAACTC CGGAGTCCGG CTGGATGGCT ACGCCCGCCT GGTACAGCAG 
ACCATCCTGT GCCACCAGAA TCCTGTGACT GGCTTACTTC CAGCAAGCTG TGATCAGAAA 
GATGCTTGGG TCCGGGATAA CGTCTACAGC ATCCTGGCGG TGTGGGGCCT GGGCCTGGCT 
TACCGCAAGA ATGCCGACCG CGACGAGGAC AAGGCCAAAG CCTATGAGTT GGAGCAGAGT 
GTCGTGAAGC TGATGAGGGG GCTGCTGCAG TGCATGATCA GACAGGTGGA CAAAGTCGAG 
TCCTTCAAAT ACAGTCAGAG CACCAAGGAT AGTCTCCACG CCAAGTACAA CACCAACACC 
TGCGCCACCG TGGTAGGAGA CGATCAATGG GGCCACTTGC AGCTGGACGC CACCTCCGTG 
TTCCTGCTCT TCCTGGCCCA AATGACAGCC TCAGGGCTCC ACATCATCCA CAGCCTAGAT 
GAGGTCAATT TCATACAGAA TCTTGTGTTT TACATCGAGT CTGCTTATAA AACAGCTGAT 
TTTGGCATAT GGGAACGTGG CGATAAGACC AACCAAGGCA TATCGGAACT GAACGCGAGC 
TCTGTCGGGA TGGCCAAGGC GGCCCTGGAA GCCTTGGATG AGCTGGACCT GTTTGGCATG 
AAGGGGGGTC CCCAGTCCGT AATTCACGTC TTGTCGGATG AGGTCCAGCA CTGCCAGTCC 
ATCCTCCATT CCATGCTGCC CCGGGCCTCG ATGTCCAAAG AGGTGGACGC CAGTGTCCTT 
TCGGTGATTT CCTTCCCAGC ATTCGCGGTA GAGGACAGTC AGCTGGTGGA GATTACGAAG 
CAAGAAATAG TCACCAAGCT TCAGGGTCGT TATGGCTGCT GTCGCTTTCT CCGAGATGGG 
TACAAAACCC CCAAAGAGGA TCCTCATCGT CTGTACTATG AGCCAGCTGA GCTGAAGCTG 
TTTGAGAACA TTGAGTGTGA ATGGCCTTTG TTCTGGACCT ACTTCATCCT GGACGGGGTC 
TTCAGTGGCA ACGTGGAGCA GGTCCAGGAG TACCGGGAAG CCCTTGAAGG GGTCCTTATC 
AAGGGCAAGA ATGGGGTTCG CCTTCTCCCT GAGCTCTATA GTGTCCCTCC TGAAAAGGTC 
GACGAAGAGT ACCAGCATCC TCACACCGTG GACCGAGTGC CCATGGGCAA GCTGCCCCAC 
ATGTGGGGCC AGTCCCTATA CATCCTGGGA AGCTTGATGG CCGAGGGGTT TTTAGCACCT 
GGAGAAATTG ATCCTCTGAA TCGCAGGTTT TCAACTGTCC CCAAGCCAGA TGTTGTGGTT 
CAAGTGTCCT TTGTAGCTGA GACAGAAGAC ATCAAGACCA TCTTGAGGAA CAATGGAATT 
GATGTGGAGA CCATTGCCGA AGTGTACCCC ATCAGAGTAC AACCAGCCCG AATCCTCAGC 
CATATCTATG CCAGCCTAGG CCGCAACAAC CGGATGAAAC TCAGTGGAAG ACCGTATCGG 
CACATGGGGG TCCTAGGAAC ATCTAAACTC TATGACATTC GTAAAACCAT CTTTGCTTTC 
ACACCACAGT TCATAGATCA GCAACAGTTC TATTTGGCCC TTGACAACAA GATGATAGTA 
GAGATGCTGC GAACAGACCT GTCCTACCTG TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACCATCACCT TTCCCATCCT GCACACCATG CTTGATGAAT CGGGAACTGC CATAAATGCA 
TCTGTCTTGA CTATGCTCAG AAAAATGCAG GATGGCTATT TCGGCGGGGC CAGGGTCCAA 
GCTGGTAAGC TGTCTGAGTT CCTGACGACA TCCTGCTGTA CACGCCTGAG CTTCATGGAT 
CCTGGCCAGG AGGGTAAGCT GTATGGTGGG AATTGTGATG ATGATGAAGA AGATGACTAT 
AATGATGAAG AGCTCTACTC GGACAACTGG CTGGGTGGCT TTGACTCCCG CAAGCAAGCA 
AGAAGGGGAA ACCCTTTTTT AAGGGGAATT GTTACTCCTG CCTCCAACCC CTCCCCAACC 
TTAGAGCTCA CTTACCTTTC CCCAAAGGAG CCAGCCTCCC GAACGGCAGT ACAAACAACC 
CATGACTACA TGTCCTTGGT GACCAAGGCT AAGGAGCTGC ATGTACAGAA TGCTCACATG 
TACCTTCCCA CAAAGTTACT GCCAGCCTCC CGAACTTCCT TCAGCTTCCT GGATGCCACG 
CCTCTGTCTC CCCAAGCTGA GCAGGTTCCT TCTGTCCACG TGGAGATGCA CCTTCCCCGG 
GACGAATCTG GGGAGATAGA CTTCCAGGCC CTGGTGGGCC AGTTGCACGC GTGCCCAAGC 
TTACAGGACC AAGCTGACAT CCTCTACGTG CTTTACACCA TGAAGGGTCC TGACTGGAAC 
ACTTCCATAG CTGGCGGTGA GGAAAGTGGG ACGGTCCAGG AGCTCCTCGT TGCCCTGTAC 
GGCAAAGCCG GGGAAGCACG CCATTGGGGC CTGATCCGCT ACATCTCTGG CATTCTGAAG 
AAGAAAGTGG AAGCCCTGGA TGAGGCCTGC ACGGATCTGC TGTCTCACCA GAAGCACCTG 
ACGGTGGGAC TCCCTCCAGA GCCACGGGAG AAGACCATCA ATGCTCCCCT ACCCTATGAA 
GAGCTCACCA AGCTGATCGA TGAAGCAAGT GAGAACGATG TAAACATCTC CATCCTCACC 
CAGGAAATCC TGGTGTACCT GGCCATGTAC ATGCGAACTC AACCCAGCCT TTTTGCGGAG 
ATGTTCCGGC TCCGGATCGG TCTCATCATC CAGGTGATGG CGACGGAACT GGTGCAGTCG 
CTTAACTGCT CAGCTCAAGA AGCCACAGAG AGCCTGATGA ACCTCAGTCC TTCGGCCATG 
AAGAACCTAT TGCACCACAT TCTCAGTGGC AAAGAGTTTG GCGTGGAAAG AAGTGTGCGT 
CCTACCGATT CCAATGTCAG CCCCGCTATT TCCATTCATG AAGTCGGCGC TGTGGGAGTG 
AGCCGAACCG AACGGGCCGG AATCATGCAG TTAAAAAGTG ACATAAAGCA GGTGGAATTA 
CGCCGGCATT CTATCTCAGT TGAGAATCAG CCCAGTGGTG GCCATGGCCT GAGTATTGAG 
CCAACATCCT CGCCTTTTCT GTCTCCCGGA CCTTCATTAA GCCCAAGTGG GAGCTCCTTC 
ACAGGCACCT TCAGAGAGCA GGCCTGGAAA GACTCTCGCC AGGGCCAGTG GCAGCGCCGG 
CGCAGGCTCG ATGGAGCCCT GAACAGGGTC CCCATCGGGT TTTATCAGAA AGTGTGGAAA 
GTCCTGCAGA AGTGCCACGG TCTCTCCGTG GAAGGCTTCG TTCTCCCTTC TTCGACGACC 
AGAGAGATGA CACCAGGGGA GATCAAGTTC TCGGTTCACG TGGAGTCCGT CCTCAACCGT 
GTTCCTCAGC CAGAATACCG GCAGCTCCTG GTTGAAGCCA TCCTCGTCCT GACCATGCTG 
GCAGACATTG AAATCCAGAG CGTAGGTGGC ATCATCGCTG TGGAAAAGAT CGTGCACATC 
GCCAACGAGC TCTTCCTCCA AGAGCAGAAA GCGCTCGGGG CAGACGACAG CATGCTGGCC 
AAGGATCCGT CGTCTGGCAT CTGTACCCTC CTGTACGACA GCGCCCCCAG TGGCAGGTTC 
GGCACCATGA CCTACCTCTC GAAAGCTGCA GCTACCTACG TGCAAGAGTT CCTGCCTCAC 
AGCGTCTGCG CCATGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031801035.1
CDS	236..3976
Translation	MRSRSNSGVRLDGYARLVQQTILCHQNPVTGLLPASCDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMIRQVDKVESFKYSQSTKDSLHAKYNTNTCATVVGDDQWGHLQLDATSVFLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIESAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGMKGGPQSVIHVLSDEVQHCQSILHSMLPRASMSKEVDASVLSVISFPAFAVEDSQLVEITKQEIVTKLQGRYGCCRFLRDGYKTPKEDPHRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNVEQVQEYREALEGVLIKGKNGVRLLPELYSVPPEKVDEEYQHPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSFVAETEDIKTILRNNGIDVETIAEVYPIRVQPARILSHIYASLGRNNRMKLSGRPYRHMGVLGTSKLYDIRKTIFAFTPQFIDQQQFYLALDNKMIVEMLRTDLSYLCSRWRMTGQPTITFPILHTMLDESGTAINASVLTMLRKMQDGYFGGARVQAGKLSEFLTTSCCTRLSFMDPGQEGKLYGGNCDDDEEDDYNDEELYSDNWLGGFDSRKQARRGNPFLRGIVTPASNPSPTLELTYLSPKEPASRTAVQTTHDYMSLVTKAKELHVQNAHMYLPTKLLPASRTSFSFLDATPLSPQAEQVPSVHVEMHLPRDESGEIDFQALVGQLHACPSLQDQADILYVLYTMKGPDWNTSIAGGEESGTVQELLVALYGKAGEARHWGLIRYISGILKKKVEALDEACTDLLSHQKHLTVGLPPEPREKTINAPLPYEELTKLIDEASENDVNISILTQEILVYLAMYMRTQPSLFAEMFRLRIGLIIQVMATELVQSLNCSAQEATESLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEVGAVGVSRTERAGIMQLKSDIKQVELRRHSISVENQPSGGHGLSIEPTSSPFLSPGPSLSPSGSSFTGTFREQAWKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIQSVGGIIAVEKIVHIANELFLQEQKALGADDSMLAKDPSSGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSVCAMQ

Target ORF information:

RefSeq Version	XM_031945175.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031945175.1

ORF Insert Sequence:

ATGAGGAGCA GGAGCAACTC CGGAGTCCGG CTGGATGGCT ACGCCCGCCT GGTACAGCAG 
ACCATCCTGT GCCACCAGAA TCCTGTGACT GGCTTACTTC CAGCAAGCTG TGATCAGAAA 
GATGCTTGGG TCCGGGATAA CGTCTACAGC ATCCTGGCGG TGTGGGGCCT GGGCCTGGCT 
TACCGCAAGA ATGCCGACCG CGACGAGGAC AAGGCCAAAG CCTATGAGTT GGAGCAGAGT 
GTCGTGAAGC TGATGAGGGG GCTGCTGCAG TGCATGATCA GACAGGTGGA CAAAGTCGAG 
TCCTTCAAAT ACAGTCAGAG CACCAAGGAT AGTCTCCACG CCAAGTACAA CACCAACACC 
TGCGCCACCG TGGTAGGAGA CGATCAATGG GGCCACTTGC AGCTGGACGC CACCTCCGTG 
TTCCTGCTCT TCCTGGCCCA AATGACAGCC TCAGGGCTCC ACATCATCCA CAGCCTAGAT 
GAGGTCAATT TCATACAGAA TCTTGTGTTT TACATCGAGT CTGCTTATAA AACAGCTGAT 
TTTGGCATAT GGGAACGTGG CGATAAGACC AACCAAGGCA TATCGGAACT GAACGCGAGC 
TCTGTCGGGA TGGCCAAGGC GGCCCTGGAA GCCTTGGATG AGCTGGACCT GTTTGGCATG 
AAGGGGGGTC CCCAGTCCGT AATTCACGTC TTGTCGGATG AGGTCCAGCA CTGCCAGTCC 
ATCCTCCATT CCATGCTGCC CCGGGCCTCG ATGTCCAAAG AGGTGGACGC CAGTGTCCTT 
TCGGTGATTT CCTTCCCAGC ATTCGCGGTA GAGGACAGTC AGCTGGTGGA GATTACGAAG 
CAAGAAATAG TCACCAAGCT TCAGGGTCGT TATGGCTGCT GTCGCTTTCT CCGAGATGGG 
TACAAAACCC CCAAAGAGGA TCCTCATCGT CTGTACTATG AGCCAGCTGA GCTGAAGCTG 
TTTGAGAACA TTGAGTGTGA ATGGCCTTTG TTCTGGACCT ACTTCATCCT GGACGGGGTC 
TTCAGTGGCA ACGTGGAGCA GGTCCAGGAG TACCGGGAAG CCCTTGAAGG GGTCCTTATC 
AAGGGCAAGA ATGGGGTTCG CCTTCTCCCT GAGCTCTATA GTGTCCCTCC TGAAAAGGTC 
GACGAAGAGT ACCAGCATCC TCACACCGTG GACCGAGTGC CCATGGGCAA GCTGCCCCAC 
ATGTGGGGCC AGTCCCTATA CATCCTGGGA AGCTTGATGG CCGAGGGGTT TTTAGCACCT 
GGAGAAATTG ATCCTCTGAA TCGCAGGTTT TCAACTGTCC CCAAGCCAGA TGTTGTGGTT 
CAAGTGTCCT TTGTAGCTGA GACAGAAGAC ATCAAGACCA TCTTGAGGAA CAATGGAATT 
GATGTGGAGA CCATTGCCGA AGTGTACCCC ATCAGAGTAC AACCAGCCCG AATCCTCAGC 
CATATCTATG CCAGCCTAGG CCGCAACAAC CGGATGAAAC TCAGTGGAAG ACCGTATCGG 
CACATGGGGG TCCTAGGAAC ATCTAAACTC TATGACATTC GTAAAACCAT CTTTGCTTTC 
ACACCACAGT TCATAGATCA GCAACAGTTC TATTTGGCCC TTGACAACAA GATGATAGTA 
GAGATGCTGC GAACAGACCT GTCCTACCTG TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACCATCACCT TTCCCATCCT GCACACCATG CTTGATGAAT CGGGAACTGC CATAAATGCA 
TCTGTCTTGA CTATGCTCAG AAAAATGCAG GATGGCTATT TCGGCGGGGC CAGGGTCCAA 
GCTGGTAAGC TGTCTGAGTT CCTGACGACA TCCTGCTGTA CACGCCTGAG CTTCATGGAT 
CCTGGCCAGG AGGGTAAGCT GTATGGTGGG AATTGTGATG ATGATGAAGA AGATGACTAT 
AATGATGAAG AGCTCTACTC GGACAACTGG CTGGGTGGCT TTGACTCCCG CAAGCAAGCA 
AGAAGGGGAA ACCCTTTTTT AAGGGGAATT GTTACTCCTG CCTCCAACCC CTCCCCAACC 
TTAGAGCTCA CTTACCTTTC CCCAAAGGAG CCAGCCTCCC GAACGGCAGT ACAAACAACC 
CATGACTACA TGTCCTTGGT GACCAAGGCT AAGGAGCTGC ATGTACAGAA TGCTCACATG 
TACCTTCCCA CAAAGTTACT GCCAGCCTCC CGAACTTCCT TCAGCTTCCT GGATGCCACG 
CCTCTGTCTC CCCAAGCTGA GCAGGTTCCT TCTGTCCACG TGGAGATGCA CCTTCCCCGG 
GACGAATCTG GGGAGATAGA CTTCCAGGCC CTGGTGGGCC AGTTGCACGC GTGCCCAAGC 
TTACAGGACC AAGCTGACAT CCTCTACGTG CTTTACACCA TGAAGGGTCC TGACTGGAAC 
ACTTCCATAG CTGGCGGTGA GGAAAGTGGG ACGGTCCAGG AGCTCCTCGT TGCCCTGTAC 
GGCAAAGCCG GGGAAGCACG CCATTGGGGC CTGATCCGCT ACATCTCTGG CATTCTGAAG 
AAGAAAGTGG AAGCCCTGGA TGAGGCCTGC ACGGATCTGC TGTCTCACCA GAAGCACCTG 
ACGGTGGGAC TCCCTCCAGA GCCACGGGAG AAGACCATCA ATGCTCCCCT ACCCTATGAA 
GAGCTCACCA AGCTGATCGA TGAAGCAAGT GAGAACGATG TAAACATCTC CATCCTCACC 
CAGGAAATCC TGGTGTACCT GGCCATGTAC ATGCGAACTC AACCCAGCCT TTTTGCGGAG 
ATGTTCCGGC TCCGGATCGG TCTCATCATC CAGGTGATGG CGACGGAACT GGTGCAGTCG 
CTTAACTGCT CAGCTCAAGA AGCCACAGAG AGCCTGATGA ACCTCAGTCC TTCGGCCATG 
AAGAACCTAT TGCACCACAT TCTCAGTGGC AAAGAGTTTG GCGTGGAAAG AAGTGTGCGT 
CCTACCGATT CCAATGTCAG CCCCGCTATT TCCATTCATG AAGTCGGCGC TGTGGGAGTG 
AGCCGAACCG AACGGGCCGG AATCATGCAG TTAAAAAGTG ACATAAAGCA GGTGGAATTA 
CGCCGGCATT CTATCTCAGT TGAGAATCAG CCCAGTGGTG GCCATGGCCT GAGTATTGAG 
CCAACATCCT CGCCTTTTCT GTCTCCCGGA CCTTCATTAA GCCCAAGTGG GAGCTCCTTC 
ACAGGCACCT TCAGAGAGCA GGCCTGGAAA GACTCTCGCC AGGGCCAGTG GCAGCGCCGG 
CGCAGGCTCG ATGGAGCCCT GAACAGGGTC CCCATCGGGT TTTATCAGAA AGTGTGGAAA 
GTCCTGCAGA AGTGCCACGG TCTCTCCGTG GAAGGCTTCG TTCTCCCTTC TTCGACGACC 
AGAGAGATGA CACCAGGGGA GATCAAGTTC TCGGTTCACG TGGAGTCCGT CCTCAACCGT 
GTTCCTCAGC CAGAATACCG GCAGCTCCTG GTTGAAGCCA TCCTCGTCCT GACCATGCTG 
GCAGACATTG AAATCCAGAG CGTAGGTGGC ATCATCGCTG TGGAAAAGAT CGTGCACATC 
GCCAACGAGC TCTTCCTCCA AGAGCAGAAA GCGCTCGGGG CAGACGACAG CATGCTGGCC 
AAGGATCCGT CGTCTGGCAT CTGTACCCTC CTGTACGACA GCGCCCCCAG TGGCAGGTTC 
GGCACCATGA CCTACCTCTC GAAAGCTGCA GCTACCTACG TGCAAGAGTT CCTGCCTCAC 
AGCGTCTGCG CCATGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_031945176.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031801036.1 Sarcophilus harrisii phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X2, mRNA.

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CloneID	OSa241368
Clone ID Related Accession (Same CDS sequence)	XM_031945176.1
Accession Version	XM_031945176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045432.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GGAGCAACTC CGGAGTCCGG CTGGATGGCT ACGCCCGCCT GGTACAGCAG 
ACCATCCTGT GCCACCAGAA TCCTGTGACT GGCTTACTTC CAGCAAGCTG TGATCAGAAA 
GATGCTTGGG TCCGGGATAA CGTCTACAGC ATCCTGGCGG TGTGGGGCCT GGGCCTGGCT 
TACCGCAAGA ATGCCGACCG CGACGAGGAC AAGGCCAAAG CCTATGAGTT GGAGCAGAGT 
GTCGTGAAGC TGATGAGGGG GCTGCTGCAG TGCATGATCA GACAGGTGGA CAAAGTCGAG 
TCCTTCAAAT ACAGTCAGAG CACCAAGGAT AGTCTCCACG CCAAGTACAA CACCAACACC 
TGCGCCACCG TGGTAGGAGA CGATCAATGG GGCCACTTGC AGCTGGACGC CACCTCCGTG 
TTCCTGCTCT TCCTGGCCCA AATGACAGCC TCAGGGCTCC ACATCATCCA CAGCCTAGAT 
GAGGTCAATT TCATACAGAA TCTTGTGTTT TACATCGAGT CTGCTTATAA AACAGCTGAT 
TTTGGCATAT GGGAACGTGG CGATAAGACC AACCAAGGCA TATCGGAACT GAACGCGAGC 
TCTGTCGGGA TGGCCAAGGC GGCCCTGGAA GCCTTGGATG AGCTGGACCT GTTTGGCATG 
AAGGGGGGTC CCCAGTCCGT AATTCACGTC TTGTCGGATG AGGTCCAGCA CTGCCAGTCC 
ATCCTCCATT CCATGCTGCC CCGGGCCTCG ATGTCCAAAG AGGTGGACGC CAGTGTCCTT 
TCGGTGATTT CCTTCCCAGC ATTCGCGGTA GAGGACAGTC AGCTGGTGGA GATTACGAAG 
CAAGAAATAG TCACCAAGCT TCAGGGTCGT TATGGCTGCT GTCGCTTTCT CCGAGATGGG 
TACAAAACCC CCAAAGAGGA TCCTCATCGT CTGTACTATG AGCCAGCTGA GCTGAAGCTG 
TTTGAGAACA TTGAGTGTGA ATGGCCTTTG TTCTGGACCT ACTTCATCCT GGACGGGGTC 
TTCAGTGGCA ACGTGGAGCA GGTCCAGGAG TACCGGGAAG CCCTTGAAGG GGTCCTTATC 
AAGGGCAAGA ATGGGGTTCG CCTTCTCCCT GAGCTCTATA GTGTCCCTCC TGAAAAGGTC 
GACGAAGAGT ACCAGCATCC TCACACCGTG GACCGAGTGC CCATGGGCAA GCTGCCCCAC 
ATGTGGGGCC AGTCCCTATA CATCCTGGGA AGCTTGATGG CCGAGGGGTT TTTAGCACCT 
GGAGAAATTG ATCCTCTGAA TCGCAGGTTT TCAACTGTCC CCAAGCCAGA TGTTGTGGTT 
CAAGTGTCCT TTGTAGCTGA GACAGAAGAC ATCAAGACCA TCTTGAGGAA CAATGGAATT 
GATGTGGAGA CCATTGCCGA AGTGTACCCC ATCAGAGTAC AACCAGCCCG AATCCTCAGC 
CATATCTATG CCAGCCTAGG CCGCAACAAC CGGATGAAAC TCAGTGGAAG ACCGTATCGG 
CACATGGGGG TCCTAGGAAC ATCTAAACTC TATGACATTC GTAAAACCAT CTTTGCTTTC 
ACACCACAGT TCATAGATCA GCAACAGTTC TATTTGGCCC TTGACAACAA GATGATAGTA 
GAGATGCTGC GAACAGACCT GTCCTACCTG TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACCATCACCT TTCCCATCCT GCACACCATG CTTGATGAAT CGGGAACTGC CATAAATGCA 
TCTGTCTTGA CTATGCTCAG AAAAATGCAG GATGGCTATT TCGGCGGGGC CAGGGTCCAA 
GCTGGTAAGC TGTCTGAGTT CCTGACGACA TCCTGCTGTA CACGCCTGAG CTTCATGGAT 
CCTGGCCAGG AGGGTAAGCT GTATGGTGGG AATTGTGATG ATGATGAAGA AGATGACTAT 
AATGATGAAG AGCTCTACTC GGACAACTGG CTGGGTGGCT TTGACTCCCG CAAGCAAGCA 
AGAAGGGGAA ACCCTTTTTT AAGGGGAATT GTTACTCCTG CCTCCAACCC CTCCCCAACC 
TTAGAGCTCA CTTACCTTTC CCCAAAGGAG CCAGCCTCCC GAACGGCAGT ACAAACAACC 
CATGACTACA TGTCCTTGGT GACCAAGGCT AAGGAGCTGC ATGTACAGAA TGCTCACATG 
TACCTTCCCA CAAAGTTACT GCCAGCCTCC CGAACTTCCT TCAGCTTCCT GGATGCCACG 
CCTCTGTCTC CCCAAGCTGA GCAGGTTCCT TCTGTCCACG TGGAGATGCA CCTTCCCCGG 
GACGAATCTG GGGAGATAGA CTTCCAGGCC CTGGTGGGCC AGTTGCACGC GTGCCCAAGC 
TTACAGGACC AAGCTGACAT CCTCTACGTG CTTTACACCA TGAAGGGTCC TGACTGGAAC 
ACTTCCATAG CTGGCGGTGA GGAAAGTGGG ACGGTCCAGG AGCTCCTCGT TGCCCTGTAC 
GGCAAAGCCG GGGAAGCACG CCATTGGGGC CTGATCCGCT ACATCTCTGG CATTCTGAAG 
AAGAAAGTGG AAGCCCTGGA TGAGGCCTGC ACGGATCTGC TGTCTCACCA GAAGCACCTG 
ACGGTGGGAC TCCCTCCAGA GCCACGGGAG AAGACCATCA ATGCTCCCCT ACCCTATGAA 
GAGCTCACCA AGCTGATCGA TGAAGCAAGT GAGAACGATG TAAACATCTC CATCCTCACC 
CAGGAAATCC TGGTGTACCT GGCCATGTAC ATGCGAACTC AACCCAGCCT TTTTGCGGAG 
ATGTTCCGGC TCCGGATCGG TCTCATCATC CAGGTGATGG CGACGGAACT GGTGCAGTCG 
CTTAACTGCT CAGCTCAAGA AGCCACAGAG AGCCTGATGA ACCTCAGTCC TTCGGCCATG 
AAGAACCTAT TGCACCACAT TCTCAGTGGC AAAGAGTTTG GCGTGGAAAG AAGTGTGCGT 
CCTACCGATT CCAATGTCAG CCCCGCTATT TCCATTCATG AAGTCGGCGC TGTGGGAGTG 
AGCCGAACCG AACGGGCCGG AATCATGCAG TTAAAAAGTG ACATAAAGCA GGTGGAATTA 
CGCCGGCATT CTATCTCAGT TGAGAATCAG TCTCCCGGAC CTTCATTAAG CCCAAGTGGG 
AGCTCCTTCA CAGGCACCTT CAGAGAGCAG GCCTGGAAAG ACTCTCGCCA GGGCCAGTGG 
CAGCGCCGGC GCAGGCTCGA TGGAGCCCTG AACAGGGTCC CCATCGGGTT TTATCAGAAA 
GTGTGGAAAG TCCTGCAGAA GTGCCACGGT CTCTCCGTGG AAGGCTTCGT TCTCCCTTCT 
TCGACGACCA GAGAGATGAC ACCAGGGGAG ATCAAGTTCT CGGTTCACGT GGAGTCCGTC 
CTCAACCGTG TTCCTCAGCC AGAATACCGG CAGCTCCTGG TTGAAGCCAT CCTCGTCCTG 
ACCATGCTGG CAGACATTGA AATCCAGAGC GTAGGTGGCA TCATCGCTGT GGAAAAGATC 
GTGCACATCG CCAACGAGCT CTTCCTCCAA GAGCAGAAAG CGCTCGGGGC AGACGACAGC 
ATGCTGGCCA AGGATCCGTC GTCTGGCATC TGTACCCTCC TGTACGACAG CGCCCCCAGT 
GGCAGGTTCG GCACCATGAC CTACCTCTCG AAAGCTGCAG CTACCTACGT GCAAGAGTTC 
CTGCCTCACA GCGTCTGCGC CATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031801036.1
CDS	236..3925
Translation	MRSRSNSGVRLDGYARLVQQTILCHQNPVTGLLPASCDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMIRQVDKVESFKYSQSTKDSLHAKYNTNTCATVVGDDQWGHLQLDATSVFLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIESAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGMKGGPQSVIHVLSDEVQHCQSILHSMLPRASMSKEVDASVLSVISFPAFAVEDSQLVEITKQEIVTKLQGRYGCCRFLRDGYKTPKEDPHRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNVEQVQEYREALEGVLIKGKNGVRLLPELYSVPPEKVDEEYQHPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSFVAETEDIKTILRNNGIDVETIAEVYPIRVQPARILSHIYASLGRNNRMKLSGRPYRHMGVLGTSKLYDIRKTIFAFTPQFIDQQQFYLALDNKMIVEMLRTDLSYLCSRWRMTGQPTITFPILHTMLDESGTAINASVLTMLRKMQDGYFGGARVQAGKLSEFLTTSCCTRLSFMDPGQEGKLYGGNCDDDEEDDYNDEELYSDNWLGGFDSRKQARRGNPFLRGIVTPASNPSPTLELTYLSPKEPASRTAVQTTHDYMSLVTKAKELHVQNAHMYLPTKLLPASRTSFSFLDATPLSPQAEQVPSVHVEMHLPRDESGEIDFQALVGQLHACPSLQDQADILYVLYTMKGPDWNTSIAGGEESGTVQELLVALYGKAGEARHWGLIRYISGILKKKVEALDEACTDLLSHQKHLTVGLPPEPREKTINAPLPYEELTKLIDEASENDVNISILTQEILVYLAMYMRTQPSLFAEMFRLRIGLIIQVMATELVQSLNCSAQEATESLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEVGAVGVSRTERAGIMQLKSDIKQVELRRHSISVENQSPGPSLSPSGSSFTGTFREQAWKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIQSVGGIIAVEKIVHIANELFLQEQKALGADDSMLAKDPSSGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSVCAMQ

Target ORF information:

RefSeq Version	XM_031945176.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031945176.1

ORF Insert Sequence:

ATGAGGAGCA GGAGCAACTC CGGAGTCCGG CTGGATGGCT ACGCCCGCCT GGTACAGCAG 
ACCATCCTGT GCCACCAGAA TCCTGTGACT GGCTTACTTC CAGCAAGCTG TGATCAGAAA 
GATGCTTGGG TCCGGGATAA CGTCTACAGC ATCCTGGCGG TGTGGGGCCT GGGCCTGGCT 
TACCGCAAGA ATGCCGACCG CGACGAGGAC AAGGCCAAAG CCTATGAGTT GGAGCAGAGT 
GTCGTGAAGC TGATGAGGGG GCTGCTGCAG TGCATGATCA GACAGGTGGA CAAAGTCGAG 
TCCTTCAAAT ACAGTCAGAG CACCAAGGAT AGTCTCCACG CCAAGTACAA CACCAACACC 
TGCGCCACCG TGGTAGGAGA CGATCAATGG GGCCACTTGC AGCTGGACGC CACCTCCGTG 
TTCCTGCTCT TCCTGGCCCA AATGACAGCC TCAGGGCTCC ACATCATCCA CAGCCTAGAT 
GAGGTCAATT TCATACAGAA TCTTGTGTTT TACATCGAGT CTGCTTATAA AACAGCTGAT 
TTTGGCATAT GGGAACGTGG CGATAAGACC AACCAAGGCA TATCGGAACT GAACGCGAGC 
TCTGTCGGGA TGGCCAAGGC GGCCCTGGAA GCCTTGGATG AGCTGGACCT GTTTGGCATG 
AAGGGGGGTC CCCAGTCCGT AATTCACGTC TTGTCGGATG AGGTCCAGCA CTGCCAGTCC 
ATCCTCCATT CCATGCTGCC CCGGGCCTCG ATGTCCAAAG AGGTGGACGC CAGTGTCCTT 
TCGGTGATTT CCTTCCCAGC ATTCGCGGTA GAGGACAGTC AGCTGGTGGA GATTACGAAG 
CAAGAAATAG TCACCAAGCT TCAGGGTCGT TATGGCTGCT GTCGCTTTCT CCGAGATGGG 
TACAAAACCC CCAAAGAGGA TCCTCATCGT CTGTACTATG AGCCAGCTGA GCTGAAGCTG 
TTTGAGAACA TTGAGTGTGA ATGGCCTTTG TTCTGGACCT ACTTCATCCT GGACGGGGTC 
TTCAGTGGCA ACGTGGAGCA GGTCCAGGAG TACCGGGAAG CCCTTGAAGG GGTCCTTATC 
AAGGGCAAGA ATGGGGTTCG CCTTCTCCCT GAGCTCTATA GTGTCCCTCC TGAAAAGGTC 
GACGAAGAGT ACCAGCATCC TCACACCGTG GACCGAGTGC CCATGGGCAA GCTGCCCCAC 
ATGTGGGGCC AGTCCCTATA CATCCTGGGA AGCTTGATGG CCGAGGGGTT TTTAGCACCT 
GGAGAAATTG ATCCTCTGAA TCGCAGGTTT TCAACTGTCC CCAAGCCAGA TGTTGTGGTT 
CAAGTGTCCT TTGTAGCTGA GACAGAAGAC ATCAAGACCA TCTTGAGGAA CAATGGAATT 
GATGTGGAGA CCATTGCCGA AGTGTACCCC ATCAGAGTAC AACCAGCCCG AATCCTCAGC 
CATATCTATG CCAGCCTAGG CCGCAACAAC CGGATGAAAC TCAGTGGAAG ACCGTATCGG 
CACATGGGGG TCCTAGGAAC ATCTAAACTC TATGACATTC GTAAAACCAT CTTTGCTTTC 
ACACCACAGT TCATAGATCA GCAACAGTTC TATTTGGCCC TTGACAACAA GATGATAGTA 
GAGATGCTGC GAACAGACCT GTCCTACCTG TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACCATCACCT TTCCCATCCT GCACACCATG CTTGATGAAT CGGGAACTGC CATAAATGCA 
TCTGTCTTGA CTATGCTCAG AAAAATGCAG GATGGCTATT TCGGCGGGGC CAGGGTCCAA 
GCTGGTAAGC TGTCTGAGTT CCTGACGACA TCCTGCTGTA CACGCCTGAG CTTCATGGAT 
CCTGGCCAGG AGGGTAAGCT GTATGGTGGG AATTGTGATG ATGATGAAGA AGATGACTAT 
AATGATGAAG AGCTCTACTC GGACAACTGG CTGGGTGGCT TTGACTCCCG CAAGCAAGCA 
AGAAGGGGAA ACCCTTTTTT AAGGGGAATT GTTACTCCTG CCTCCAACCC CTCCCCAACC 
TTAGAGCTCA CTTACCTTTC CCCAAAGGAG CCAGCCTCCC GAACGGCAGT ACAAACAACC 
CATGACTACA TGTCCTTGGT GACCAAGGCT AAGGAGCTGC ATGTACAGAA TGCTCACATG 
TACCTTCCCA CAAAGTTACT GCCAGCCTCC CGAACTTCCT TCAGCTTCCT GGATGCCACG 
CCTCTGTCTC CCCAAGCTGA GCAGGTTCCT TCTGTCCACG TGGAGATGCA CCTTCCCCGG 
GACGAATCTG GGGAGATAGA CTTCCAGGCC CTGGTGGGCC AGTTGCACGC GTGCCCAAGC 
TTACAGGACC AAGCTGACAT CCTCTACGTG CTTTACACCA TGAAGGGTCC TGACTGGAAC 
ACTTCCATAG CTGGCGGTGA GGAAAGTGGG ACGGTCCAGG AGCTCCTCGT TGCCCTGTAC 
GGCAAAGCCG GGGAAGCACG CCATTGGGGC CTGATCCGCT ACATCTCTGG CATTCTGAAG 
AAGAAAGTGG AAGCCCTGGA TGAGGCCTGC ACGGATCTGC TGTCTCACCA GAAGCACCTG 
ACGGTGGGAC TCCCTCCAGA GCCACGGGAG AAGACCATCA ATGCTCCCCT ACCCTATGAA 
GAGCTCACCA AGCTGATCGA TGAAGCAAGT GAGAACGATG TAAACATCTC CATCCTCACC 
CAGGAAATCC TGGTGTACCT GGCCATGTAC ATGCGAACTC AACCCAGCCT TTTTGCGGAG 
ATGTTCCGGC TCCGGATCGG TCTCATCATC CAGGTGATGG CGACGGAACT GGTGCAGTCG 
CTTAACTGCT CAGCTCAAGA AGCCACAGAG AGCCTGATGA ACCTCAGTCC TTCGGCCATG 
AAGAACCTAT TGCACCACAT TCTCAGTGGC AAAGAGTTTG GCGTGGAAAG AAGTGTGCGT 
CCTACCGATT CCAATGTCAG CCCCGCTATT TCCATTCATG AAGTCGGCGC TGTGGGAGTG 
AGCCGAACCG AACGGGCCGG AATCATGCAG TTAAAAAGTG ACATAAAGCA GGTGGAATTA 
CGCCGGCATT CTATCTCAGT TGAGAATCAG TCTCCCGGAC CTTCATTAAG CCCAAGTGGG 
AGCTCCTTCA CAGGCACCTT CAGAGAGCAG GCCTGGAAAG ACTCTCGCCA GGGCCAGTGG 
CAGCGCCGGC GCAGGCTCGA TGGAGCCCTG AACAGGGTCC CCATCGGGTT TTATCAGAAA 
GTGTGGAAAG TCCTGCAGAA GTGCCACGGT CTCTCCGTGG AAGGCTTCGT TCTCCCTTCT 
TCGACGACCA GAGAGATGAC ACCAGGGGAG ATCAAGTTCT CGGTTCACGT GGAGTCCGTC 
CTCAACCGTG TTCCTCAGCC AGAATACCGG CAGCTCCTGG TTGAAGCCAT CCTCGTCCTG 
ACCATGCTGG CAGACATTGA AATCCAGAGC GTAGGTGGCA TCATCGCTGT GGAAAAGATC 
GTGCACATCG CCAACGAGCT CTTCCTCCAA GAGCAGAAAG CGCTCGGGGC AGACGACAGC 
ATGCTGGCCA AGGATCCGTC GTCTGGCATC TGTACCCTCC TGTACGACAG CGCCCCCAGT 
GGCAGGTTCG GCACCATGAC CTACCTCTCG AAAGCTGCAG CTACCTACGT GCAAGAGTTC 
CTGCCTCACA GCGTCTGCGC CATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PHKA1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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