NF1 gene cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

mRNA	Protein	Name
XM_031967655.1	XP_031823515.1	neurofibromin isoform X16
XM_031967640.1	XP_031823500.1	neurofibromin isoform X2
XM_031967644.1	XP_031823504.1	neurofibromin isoform X6
XM_031967648.1	XP_031823508.1	neurofibromin isoform X9
XM_031967650.1	XP_031823510.1	neurofibromin isoform X11
XM_031967653.1	XP_031823513.1	neurofibromin isoform X14
XM_031967639.1	XP_031823499.1	neurofibromin isoform X1
XM_031967645.1	XP_031823505.1	neurofibromin isoform X7
XM_031967651.1	XP_031823511.1	neurofibromin isoform X12
XM_031967654.1	XP_031823514.1	neurofibromin isoform X15
XM_031967656.1	XP_031823516.1	neurofibromin isoform X17
XM_023503611.1	XP_023359379.1	neurofibromin
XM_003770020.2	XP_003770068.2	neurofibromin
XM_031967641.1	XP_031823501.1	neurofibromin isoform X3
XM_031967642.1	XP_031823502.1	neurofibromin isoform X4
XM_031967657.1	XP_031823517.1	neurofibromin isoform X18
XM_031967643.1	XP_031823503.1	neurofibromin isoform X5
XM_031967647.1	XP_031823507.1	neurofibromin isoform X8
XM_031967649.1	XP_031823509.1	neurofibromin isoform X10
XM_031967652.1	XP_031823512.1	neurofibromin isoform X13

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa132231	XM_003770020.2 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234688	XM_031967639.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234689	XM_031967640.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234690	XM_031967641.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234691	XM_031967642.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234692	XM_031967643.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234693	XM_031967644.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234694	XM_031967645.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234695	XM_031967647.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234696	XM_031967648.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234697	XM_031967649.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234698	XM_031967650.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234699	XM_031967651.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234700	XM_031967652.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234701	XM_031967653.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234702	XM_031967654.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234703	XM_031967655.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234704	XM_031967656.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa234705	XM_031967657.1 Latest version!	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

NF1 ( XM_003770020.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003770068.2 Sarcophilus harrisii neurofibromin 1 (NF1), mRNA.

Price & Availability↑

CloneID	OSa132231
Clone ID Related Accession (Same CDS sequence)	XM_003770020.2
Accession Version	XM_003770020.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003839010.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 15, 2015 this sequence version replaced XM_003770020.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGAAAGAAG TGGAAGACAT GGCCAAGAAT TTAGTGTGTT CTGGGGAGAG CTCGCTTCCA 
ATTAAAGCTG GACAGCAGAC TACACACACC AAAGTCAGCA CTGAACACAA CAAGGAGTGT 
CTTATCAACA TTTCCAAATA CAAGTTTTCT CTGGTTATAA GTGGCCTCAC CAATATTTTA 
AAAAATGTTA ACAATATGAG AATATTTGGT GAAGCTGCTG AAAAAAATTT ATATCTCTCT 
CAGTTAATTA TTTTGGATAC ACTGGAAAAA TGCCTTGCTG GGCAGCCAAA GGACACGATG 
AGGTTAGATG AAACAATGCT GGTCAAACAG TTGCTGCCGG AAATCTGCCA TTTCCTTCAC 
ACCTACCGGG AAGGGAACCA GCACGCAGCC GAGCTTCGGA GCTCCGCCTC TGGCGTCCTT 
TTCTCCCTCA GCTGTAATAA CTTCAATGCC GTCTTCAGCC GGATTTCCAC CAGGTTACAG 
GAATTGACTG TTTGCTCAGA AGATAATGTT GATGTCCATG ATATAGAATT ATTACAATAT 
ATCAATGTGG ATTGTGCAAA ACTAAAGCGG CTCTTGCAGG AAACTGTATT TAAGTTTAAA 
GCCTTAAAGA AGGTAGCTCA ACTAGCAGTT ATAAATAGCC TAGAAAAGGC ATTTTGGAAT 
TGGGTAGAGA ACTATCCTGA TGAATTTACA AAGCTGTACC AGACTCCCCA GACAGATATG 
GCGGAATGTG CAGAAAAATT ATTTGACTTG GTGGATGGTT TTGCTGAAAG CACCAAACGT 
AAAGCTGCAG TTTGGCCACT GCAAATCATT CTTCTTGTCT TGTGTCCAGA AATAATCCAG 
GACATTTCCA AGGATGTGGT TGATGAAGCG AAAATGAATA AGAAAATGTT TCTCGACAAT 
CTCAGAAGAG CGCTGGCCGG CCACAGTGGG AGCAGGCAGC TGACAGAGAG TGCCGCGATC 
GCTTGTGTTA AACTGTGCAA AGCCAGCACC TACATCAACT GGGAGGATAA CTCGGTCATT 
TTCCTGCTCG TCCAATCCAT GGTGGTGGAT CTGAAGAACT TGCTCTTTAA TCCCAGTAAG 
CCCTTCTCCA GAGGCAGCCA GAATGCTGAT GTGGACCTCA TGATAGACTG CCTGGTTTCT 
TGCTTTCGCA TCAGCCCTCA CAACAACCAA CACTTTAAGA TCTGTTTGGC CCAGAACTCC 
CCATCTACAT TTCATTATGT GCTGGTAAAC TCCCTCCATC GAATTATCAC CAATTCCGCC 
CTGGATTGGT GGCCCAAGAT TGATGCTGTG TATTGCCATT CTGTAGAACT TCGAAACATG 
TTTGGTGAAA CGCTTCACAA GGCCGTGCAG GGCTGTGGGC CTCACCCCGC GATACGAATG 
GCGCCGAGTC TTACATTTAA AGAAAAAATG ACAAGCCTTA AATTTAAAGA AAAACCTACG 
GACTTGGAAA CAAGAAGTTA TAAATACCTC TTATTATCCA TGGTGAAACT AATCCATGCA 
GATCCAAAGC TGTTGCTATG TAATCCAAGA AAACAAGGGC CTGAAACTCA GGGGAGTACA 
GCAGAGTTAA TCACAGGCCT TGTGCAGCTC GTCCCACAGT CCAGCATGCC AGAAATAGCT 
CAAGAAGCAA TGGAGGCTTT GCTGGTTCTT CATCAGTTGG ATAGTATTGA CTTATGGAAT 
CCAGATTCAC CTATAGAAAC ATTCTGGGAG ATTAGCTCAC AAATGCTTTT TTATATCTGT 
AAAAAGCTAA CTACCCATCA GATGCTTAGC AGTACAGAAA TTCTCAAGTG GTTGCGAGAA 
ATTTTGATCT GTAGGAACAA ATATCTTCTT AAAAATAAGC AGTCAGATAG GAGTTCTTCC 
CATTTCCTCT ACCTTTATGG GGCAGGCTAT GATGTCTCTT CTGGTGGAGG TACCAGTCAT 
ATCTCTCTAG ACCACGAGGA GTTTATGCGT ACCTGTGCTC ATGGACCATC TCTCAGGAAA 
GTGAAAGGAA GCTCCTCAAT GGACGGTGCA ATCGGGTCCA GCGGGACGCC CATCTGCCGC 
CAGGCCCAGA CTAAGCTGGA GGTTGCTCTG TACATGTTTC TGTGGAGTCC CGACATTGAA 
GCCGTGCTCG TGGCCATGTC CTGCTTTCGA CACCTGTGTG AGGAAGCCGA CATCAGGTGT 
GGCATAGATG AGATCTCGGT GCACCATTTC TTGCCAAACT ATAACACCTT CATGGAGTTC 
GCATCTGTCA GCAACATGAT GTCGACAGGA AGAGCAGCTC TTCAGAAAAG AGTGATGGCC 
CTGCTCAGGC GCATCGAACA CCCCACCGCT GGAAACACCG AGGCTTGGGA AGATACTTAC 
GCCAAATGGG AGCAAGCTAC AAAATCAATC CTCAATTATC CGAAAGCCAA AATTGAAGAT 
GGCCAGGTTG CTGAGAGTCT TCACAAGACC ATTGTGAAGA GAAGAATGTC TCACGTGAGT 
GGAGGGGGCT CCATAGACTT GTGCGACACT GACTCGCTAC AGGAGTGGAT CAACATGACT 
GGGTTTCTCT GTGCCCTTGG TGGCGTGTGC CTACAGCAGC GGAGCAATGC CGGCTTTGCC 
ACCTACAGCC CTCCCATGGG CCCTATCAGT GAACGCAAGG GCTCTCTCAT TTCCATGGTG 
TCCAGCGATG GAAACGCAGA GACGCCTGTC AGCAAGTTCA TGGATCGGCT GCTCTCCTTG 
ATGGTGTGTA ACCACGAAAA AGTGGGGCTG CAGATACGAA GCAATGTCAA GGACTTGTTG 
GGTCTTGAAT TGAGTCCTGC CCTTTATCCA ATGCTGTTTA ACAAATTAAA AAATACCATC 
AGCAAGTTTT TTGATCCTCA AGGACAGGTT TTATTGACTG ACACCAACAC CCAGTTTGTG 
GAGCAGACCA TAGCCATAAT GAAGAACTTG CTTGATAATC ATGCTGAGGG TAGTTCTGAA 
CACATGGGAC AAGCTAGCAT CGAAACTATG ATGTTAAATC TGGTCAGATA TGTTAGAGTG 
TTTGGGAATT TGGTGCAGGC AATTCAGATC AAAACTAAGC TGTGCCAGCT GGTTCAAGTA 
ATGATGGAGA GGAGAGATGA CCTTTCATTT TGCCAAGAGA TGAAATTTAG GAATAAAATG 
GTAGAATATC TGACAGATTG GGTCATGGGA ACATCTAACC AAGCTGCAGA TGAAGATGTG 
AAATGTCTAA CTAGAGATTT GGACCAGGCA AGCATGGAAG CAGTGGTGTC ATTGCTCGCT 
GGGCTCCCTC TGCAGCCTGA GGAAGGCGAT GGGGTGGAAT TGATGGAAGC CAAATCCCAG 
CTGTTCCTTA AGTATTTCAC CCTCTTTATG AACCTTCTTA ATGACTGCAG TGAGGTCGAA 
GAAGAAAATG TCCAGATTGG GGGCAGGAAG CGCGGCATGT CGCGAAGGCT GGCGTCCCTG 
CGCCACTGCA CCGTGCTGGC AATGTCCAAC CTGCTCAATG CGAATGTAGA CAGCGGCCTG 
ATGCACTCTA TAGGATTGGG TTACCACAAA GATCTTCAGA CAAGAGCCAC CTTCATGGAA 
GTCCTCACAA AAATCCTTCA GCAAGGCACA GAGTTTGATA CGCTCGCAGA AACCGTGTTA 
GCTGATCGAT TTGAGAGACT TGTTGAGTTG GTCACGATGA TGGGTGATCA AGGAGAGCTC 
CCCATAGCCA TGGCTCTGGC CAACGTGGTG CCTTGTTCTC AGTGGGATGA ACTTGCTCGA 
GTCCTTGTTA CTCTCTTTGA TTCCCGACAC TTACTGTATC AGCTCCTTTG GAACATGTTT 
TCTAAGGAAG TTGAGCTGGC AGACTCCATG CAGACACTTT TTAGAGGAAA CAGCTTGGCC 
AGTAAAATCA TGACCTTCTG CTTTAAGGTC TACGGTGCTA CATACCTGCA GAAGCTTCTG 
GAGCCTTTAC TCCGCTTTGT GATCACATCC TCCGAATGGC AGCACGTCAG CTTCGAGGTC 
GATCCCACCA GGTTAGAGCA TACTGAGAGC CTCGAGGACA ACCAGCGGAA CCTTTTGCAA 
ATGACTGAGA AGTTCTTCCA CGCCATCATC AGTTCTTCCT CCGAGTTCCC CCCTCAGCTA 
CGCAGTGTGT GCCACTGCCT GTACCAGGCA ACTTGCCACT CTCTACTGAA TAAAGCTACC 
GTAAAAGAAA AAAAGGAAAA CAAAAAATCA GTTGTTAGCC AGAGATTCCC TCAGAACAGC 
ATTGGCGCCG TGGGGAGTGC CATGTTCCTG AGATTCATCA ACCCTGCCGT TGTCTCTCCC 
TACGAGGCAG GGATTTTAGA GAGAAAACCG CTCCCTAGAA TTGAAAGGGG GTTGAAGCTG 
ATGTCAAAGA TTCTTCAGAG CATTGCCAAT CATGTCCTTT TCACAAAAGA AGAGTATATG 
AGGCCTTTTA ACGATTTTGT GAAAAGCAAC TTTGATGTAG CACGGAGATT TTTCCTTGAT 
ATCGCATCTG ATTGTCCTGT AAGTGATGCC ATAAACCACA GCCTTTCCTT CATCAGCGAT 
GGCAATGTCT TGGCTTTGCA CCGTCTGCTC TGGAACAACC AAGAGAAAAT CGGCCAATAT 
CTTTCAAGCA ACAGAGATCA CAAAGCTGTT GGAAGGCGAC CTTTCGATAA GATGGCAACA 
CTGCTTGCCT ACCTGGGACC TCCAGAGCAC AAGCCTGTGG CTGACACACA CTGGTCCAGC 
CTAAATCTTA CCAGCTCCAA GTTTGAGGAA TTCATGACAA GGCACCAGGT ACATGAAAAA 
GAGGAATTCA AGGCTCTGAA AACATTAAAT ATCTTCTATC AAGCAGGCAC TTCCAAAGCT 
GGAAATCCTG TTTTTTATTA TGTCACACGA AGGTTCAAGA CTGGCCAGAT CAACGGGGAT 
TTGCTTATAT ACCACGTCTT ACTAACTTTA AAGCCATATT ATGCAAAGCC ATATGAGATT 
GTAATGGACC TCACTCATGC CGGGCCTAGC AATCGCTTTA AGACAGACTT CCTCTCCAAG 
TGGTTTGTTG TTTTCCCGGG CTTTGCTTAC GAGAACGTCT CTTCGGTCTA CATCTACAAC 
TGTAATTCTT GGGTTCGGGA GTACACCAAA TACCATGAGA GACTGCTCAC GGGACTCAAA 
GGCAGCAAAA GACTCTTTTT CATAGACTCT CCCTCAAAAC TGGCTGAGCA TATCGATTAT 
GATCAGCAAA AACTGCCAGC CGCGACCTTG GCTCTGGATG AAGACCTCAA GGTGTACAAC 
AGTGCTCTCA AGCTGGCTCA CAAGGACACC AAGGTGACCC TCAAGGTTGG CTCTACTGCG 
ATCCAAGTGA CGTCATCTGA GCGAACTAAA GTCCTGGGAC AGACGGTTTT TCTGAATGAT 
ATTTACTATG CCTCTGAGAT CGAAGAAATC TGTCTTGTGG ATGAAAACCA GTTCACTCTG 
ACCATTTCCA ATCAGGGCAC TCCCCTCACC TTCATGCATC AAGAGTGTGA AGGCATTGTG 
GCGTCCATCA TTCACATCCG AACTCGATGG GAGCTGTCCC AGCCAGATTC TATCCCCCAG 
CACACTAAGA TCCGGCCAAA GGACGTTCCC GGAACACTTT TGAATATCGC ATTACTTAAC 
TTGGGTAGTT CGGACCCAAG TTTACGGTCA GCCGCCTATA ATCTTCTCTG TGCCTTAACC 
TGTACCTTTA ATTTAAAAAT TGAGGGCCAA TTACTAGAAA CATCAGGTCT GTGTATACCT 
GCCAACAATA CCCTTTTTAT TGTCTCTATT AGTAAAACCC TGGCAGCTAA TGAGCCACAC 
CTCACCTTAG AGTTTTTGGA AGAGTGCATT TCCGGATTTA GCAAATCCAG TATTGAACTG 
AAGCACCTGT GTCTAGAATA CATGACTCCC TGGCTATCAA ATCTAGTCCG TTTCTGTAAA 
CATAATGATG ATGCCAAACG ACAGAGAGTC ACTGCTATTC TTGATAAACT TATAACAATG 
ACCATAAATG AAAAGCAGAT GTACCCATCG ATCCAAGCCA AGATATGGGG AAGCCTTGGG 
CAGATTACAG ATCTGCTCGA TGTAGTGCTG GACAGCTTTA TCAAAACCAG TGCCACAGGT 
GGTTTAGGAT CCATCAAGGC TGAAGTGATG GCTGACACGG CCGTGGCTCT GGCTTCTGGG 
AACGTCAAAC TAGTTTCGAG CAAGGTGATT GGAAGAATGT GCAAAATCAT TGACAAGACC 
TGCCTCTCTC CAACTCCTAC CTTAGAGCAG CACCTCATGT GGGATGATAT CGCCATTTTA 
GCACGCTACA TGCTAATGCT GTCCTTCAAT AATTCTCTGG ATGTGGCGGC TCATCTTCCC 
TACCTCTTCC ATGTGGTGAC TCTGTTGGTG GCCACAGGTC CCTTGTCCCT TCGTGCTTCC 
ACACACGGAC TGGTCATCAA TATCATCCAT TCTCTGTGCA CGTGCTCACA GCTTCACTTT 
AGTGAAGAGA CCAAGCAAGT TTTACGACTG AGTCTGACTG AGTTCTCACT ACCTAAGTTT 
TATTTGCTGT TCGGCATCAG CAAAGTCAAA TCAGCTGCCG TGATAGCCTT CCGGTCCAGT 
TACCGGGACA GGTCTTTCTC CCCTGGCTCC TATGAGAGGG AGACCTTTGC CCTGGCCTCC 
TTGGAAACAG TCACAGAAGC TCTCTTGGAG ATCATGGAGG CTTGTATGAG AGATATTCCT 
GCATGCAAGT GGCTGGACCA GTGGACAGAG CTAGCCCAGA GGTTTGCGTT CCAGTACAAT 
CCCTCCCTGC AGCCACGAGC TCTGGTTGTG TTTGGTTGTA TCAGCAAAAG AGTGTCCCAT 
GGGCAGATCA AGCAGATTAT CCGCATTCTT AGCAAGGGAC TTGAGAGCTG CCTAAAAGGA 
CCTGATAATT ATAACAGTCA AGTTCTGATT GAAGCTACCG TGATAGCTCT AACCAAATTA 
CAACCACTTC TTAATAAGNN NNNCCCTCTG CACCGAGCTC TCTTCTGGGT GGCCGTTGCC 
GTGCTGCAGC TGGATGAAGT GGACCTGTAC TCGGCGGGCA CTGCCCTCCT CGAACAGAAC 
CTGCACACCT TAGACAGCCT CCTGCACATC TTCAACGACC AGAGCCCAGA AGAAGTGTTC 
ATGGAGATCC GGCAGCCTCT GGAGTGGCAC TGCAAGCAGA TGGACCACTT TGTGGGGCTC 
AACTTTAACT CCAGCTTTAA CTTTGCCCTG GTCGGGCACC TGCTGAAGGG ATTTCGGCAT 
CCTTCACCTG TCACTGTTGC AAGAACTGTG AGGATTTTGC ACACCCTACT AGCATTGGTT 
AACAAGCATA GGAATTGTGA CAAGTTTGAG GTGAACACCC AGAGTGTGGC TTACTTAGCA 
GCTTTACTTA CTGTGTCTGA GGAGGTTCGA AGTCGCTGCA GCTTAAAGCA TAGGAAATCA 
CTCCTACTAA CTGATATCAC AATGGAAAAT GTGCCTATGG ATATATATCC CATCTATCAC 
AGTGACCCCA GCCTTAGGAC ACTAAAAGAA ACACAACCGT GGTCCTCTCC AAAGGGATCT 
GAGAGACATC TTGCAGCCAG CTACCCTCCT GTGGGCCAAA CTAGCCCCCG AACCAGAAAA 
TCCATGAGCC TGGATATGGG GCAGCCTTCC CAGGCCAACA CAAAGAAGTT GCTTGGGACA 
AGGAAAAGCT TCGATCATTT GATCTCTGAT GCCAAAGCTC CCAAAAGGCA AGAACAGGAG 
TCTGGAGTCA CCACGCCCCC CAAGATGCGG AGAGTGGCCG AGAACGACTA CGAGATGGAA 
ACCCAGAGAA TCTCCCCTCA GCCACACCCG CCTCTGCGGA AAGTGTCCGT GTCCGAATCC 
AATGTGCTGC TGGACGAGGA AGTGCTCACA GACCCCAAAA CCCAGGCTCT TCTTCTCACT 
GTCCTGGCTA CTCTGGTGAA GTACACTGCG GACGAGTTTG ACCAGCGCAT TCTCTATGAG 
TACTTAGCGG AGGCCAGTGT TGTCTTCCCA AAGGTTTTTC CAGTGGTACA TAATTTGTTG 
GACTCAAAGA TAAATACTCT GCTCTCATTA TGCCAAGACC CAAATTTGCT AAACCCGGTT 
CATGGAATTG TGCAGAGTGT GGTCTACCAC GAAGAGGCCC CGCCGCAGTA CCAGACGGCG 
TACCTCCAGA GTTTCGGTTT TAATGGTCTG TGGAGGTTTG CAGGACCATT TTCTAAGCAG 
ACGCAGATCC CAGACTATGC CGAGCTCATC GTGAAGTTTC TCGATGCCTT GATTGACACG 
TATCTGCCTG GAATTGATGA GGAAGCCAGT GAGGAGTCCC TCCTGACACC CACCTCTCCC 
TACCCGCCCG CGGTGCAGAG CCAGCTTAGC ATCACTGCGA ACCTCAACCT CTCTAACTCC 
ATGACCTCAC TGGCAACTTC CCAGCATTCC CCAGGCATCG ACAAGGAGAA TGTGGAGCTC 
TCGCCAACCA CCGGGCACTG CAGCAGGGTC ATTGGGGATC AAGGAAGCCA GCGTGGCCGA 
GAGGCCACTT TTTACGTCTC CCGTTTGGCC AAGGGCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003770068.2
CDS	1..8502
Translation	MKEVEDMAKNLVCSGESSLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKnullnullPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSRVIGDQGSQRGREATFYVSRLAKGY

Target ORF information:

RefSeq Version	XM_003770020.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003770020.2

ORF Insert Sequence:

ATGAAAGAAG TGGAAGACAT GGCCAAGAAT TTAGTGTGTT CTGGGGAGAG CTCGCTTCCA 
ATTAAAGCTG GACAGCAGAC TACACACACC AAAGTCAGCA CTGAACACAA CAAGGAGTGT 
CTTATCAACA TTTCCAAATA CAAGTTTTCT CTGGTTATAA GTGGCCTCAC CAATATTTTA 
AAAAATGTTA ACAATATGAG AATATTTGGT GAAGCTGCTG AAAAAAATTT ATATCTCTCT 
CAGTTAATTA TTTTGGATAC ACTGGAAAAA TGCCTTGCTG GGCAGCCAAA GGACACGATG 
AGGTTAGATG AAACAATGCT GGTCAAACAG TTGCTGCCGG AAATCTGCCA TTTCCTTCAC 
ACCTACCGGG AAGGGAACCA GCACGCAGCC GAGCTTCGGA GCTCCGCCTC TGGCGTCCTT 
TTCTCCCTCA GCTGTAATAA CTTCAATGCC GTCTTCAGCC GGATTTCCAC CAGGTTACAG 
GAATTGACTG TTTGCTCAGA AGATAATGTT GATGTCCATG ATATAGAATT ATTACAATAT 
ATCAATGTGG ATTGTGCAAA ACTAAAGCGG CTCTTGCAGG AAACTGTATT TAAGTTTAAA 
GCCTTAAAGA AGGTAGCTCA ACTAGCAGTT ATAAATAGCC TAGAAAAGGC ATTTTGGAAT 
TGGGTAGAGA ACTATCCTGA TGAATTTACA AAGCTGTACC AGACTCCCCA GACAGATATG 
GCGGAATGTG CAGAAAAATT ATTTGACTTG GTGGATGGTT TTGCTGAAAG CACCAAACGT 
AAAGCTGCAG TTTGGCCACT GCAAATCATT CTTCTTGTCT TGTGTCCAGA AATAATCCAG 
GACATTTCCA AGGATGTGGT TGATGAAGCG AAAATGAATA AGAAAATGTT TCTCGACAAT 
CTCAGAAGAG CGCTGGCCGG CCACAGTGGG AGCAGGCAGC TGACAGAGAG TGCCGCGATC 
GCTTGTGTTA AACTGTGCAA AGCCAGCACC TACATCAACT GGGAGGATAA CTCGGTCATT 
TTCCTGCTCG TCCAATCCAT GGTGGTGGAT CTGAAGAACT TGCTCTTTAA TCCCAGTAAG 
CCCTTCTCCA GAGGCAGCCA GAATGCTGAT GTGGACCTCA TGATAGACTG CCTGGTTTCT 
TGCTTTCGCA TCAGCCCTCA CAACAACCAA CACTTTAAGA TCTGTTTGGC CCAGAACTCC 
CCATCTACAT TTCATTATGT GCTGGTAAAC TCCCTCCATC GAATTATCAC CAATTCCGCC 
CTGGATTGGT GGCCCAAGAT TGATGCTGTG TATTGCCATT CTGTAGAACT TCGAAACATG 
TTTGGTGAAA CGCTTCACAA GGCCGTGCAG GGCTGTGGGC CTCACCCCGC GATACGAATG 
GCGCCGAGTC TTACATTTAA AGAAAAAATG ACAAGCCTTA AATTTAAAGA AAAACCTACG 
GACTTGGAAA CAAGAAGTTA TAAATACCTC TTATTATCCA TGGTGAAACT AATCCATGCA 
GATCCAAAGC TGTTGCTATG TAATCCAAGA AAACAAGGGC CTGAAACTCA GGGGAGTACA 
GCAGAGTTAA TCACAGGCCT TGTGCAGCTC GTCCCACAGT CCAGCATGCC AGAAATAGCT 
CAAGAAGCAA TGGAGGCTTT GCTGGTTCTT CATCAGTTGG ATAGTATTGA CTTATGGAAT 
CCAGATTCAC CTATAGAAAC ATTCTGGGAG ATTAGCTCAC AAATGCTTTT TTATATCTGT 
AAAAAGCTAA CTACCCATCA GATGCTTAGC AGTACAGAAA TTCTCAAGTG GTTGCGAGAA 
ATTTTGATCT GTAGGAACAA ATATCTTCTT AAAAATAAGC AGTCAGATAG GAGTTCTTCC 
CATTTCCTCT ACCTTTATGG GGCAGGCTAT GATGTCTCTT CTGGTGGAGG TACCAGTCAT 
ATCTCTCTAG ACCACGAGGA GTTTATGCGT ACCTGTGCTC ATGGACCATC TCTCAGGAAA 
GTGAAAGGAA GCTCCTCAAT GGACGGTGCA ATCGGGTCCA GCGGGACGCC CATCTGCCGC 
CAGGCCCAGA CTAAGCTGGA GGTTGCTCTG TACATGTTTC TGTGGAGTCC CGACATTGAA 
GCCGTGCTCG TGGCCATGTC CTGCTTTCGA CACCTGTGTG AGGAAGCCGA CATCAGGTGT 
GGCATAGATG AGATCTCGGT GCACCATTTC TTGCCAAACT ATAACACCTT CATGGAGTTC 
GCATCTGTCA GCAACATGAT GTCGACAGGA AGAGCAGCTC TTCAGAAAAG AGTGATGGCC 
CTGCTCAGGC GCATCGAACA CCCCACCGCT GGAAACACCG AGGCTTGGGA AGATACTTAC 
GCCAAATGGG AGCAAGCTAC AAAATCAATC CTCAATTATC CGAAAGCCAA AATTGAAGAT 
GGCCAGGTTG CTGAGAGTCT TCACAAGACC ATTGTGAAGA GAAGAATGTC TCACGTGAGT 
GGAGGGGGCT CCATAGACTT GTGCGACACT GACTCGCTAC AGGAGTGGAT CAACATGACT 
GGGTTTCTCT GTGCCCTTGG TGGCGTGTGC CTACAGCAGC GGAGCAATGC CGGCTTTGCC 
ACCTACAGCC CTCCCATGGG CCCTATCAGT GAACGCAAGG GCTCTCTCAT TTCCATGGTG 
TCCAGCGATG GAAACGCAGA GACGCCTGTC AGCAAGTTCA TGGATCGGCT GCTCTCCTTG 
ATGGTGTGTA ACCACGAAAA AGTGGGGCTG CAGATACGAA GCAATGTCAA GGACTTGTTG 
GGTCTTGAAT TGAGTCCTGC CCTTTATCCA ATGCTGTTTA ACAAATTAAA AAATACCATC 
AGCAAGTTTT TTGATCCTCA AGGACAGGTT TTATTGACTG ACACCAACAC CCAGTTTGTG 
GAGCAGACCA TAGCCATAAT GAAGAACTTG CTTGATAATC ATGCTGAGGG TAGTTCTGAA 
CACATGGGAC AAGCTAGCAT CGAAACTATG ATGTTAAATC TGGTCAGATA TGTTAGAGTG 
TTTGGGAATT TGGTGCAGGC AATTCAGATC AAAACTAAGC TGTGCCAGCT GGTTCAAGTA 
ATGATGGAGA GGAGAGATGA CCTTTCATTT TGCCAAGAGA TGAAATTTAG GAATAAAATG 
GTAGAATATC TGACAGATTG GGTCATGGGA ACATCTAACC AAGCTGCAGA TGAAGATGTG 
AAATGTCTAA CTAGAGATTT GGACCAGGCA AGCATGGAAG CAGTGGTGTC ATTGCTCGCT 
GGGCTCCCTC TGCAGCCTGA GGAAGGCGAT GGGGTGGAAT TGATGGAAGC CAAATCCCAG 
CTGTTCCTTA AGTATTTCAC CCTCTTTATG AACCTTCTTA ATGACTGCAG TGAGGTCGAA 
GAAGAAAATG TCCAGATTGG GGGCAGGAAG CGCGGCATGT CGCGAAGGCT GGCGTCCCTG 
CGCCACTGCA CCGTGCTGGC AATGTCCAAC CTGCTCAATG CGAATGTAGA CAGCGGCCTG 
ATGCACTCTA TAGGATTGGG TTACCACAAA GATCTTCAGA CAAGAGCCAC CTTCATGGAA 
GTCCTCACAA AAATCCTTCA GCAAGGCACA GAGTTTGATA CGCTCGCAGA AACCGTGTTA 
GCTGATCGAT TTGAGAGACT TGTTGAGTTG GTCACGATGA TGGGTGATCA AGGAGAGCTC 
CCCATAGCCA TGGCTCTGGC CAACGTGGTG CCTTGTTCTC AGTGGGATGA ACTTGCTCGA 
GTCCTTGTTA CTCTCTTTGA TTCCCGACAC TTACTGTATC AGCTCCTTTG GAACATGTTT 
TCTAAGGAAG TTGAGCTGGC AGACTCCATG CAGACACTTT TTAGAGGAAA CAGCTTGGCC 
AGTAAAATCA TGACCTTCTG CTTTAAGGTC TACGGTGCTA CATACCTGCA GAAGCTTCTG 
GAGCCTTTAC TCCGCTTTGT GATCACATCC TCCGAATGGC AGCACGTCAG CTTCGAGGTC 
GATCCCACCA GGTTAGAGCA TACTGAGAGC CTCGAGGACA ACCAGCGGAA CCTTTTGCAA 
ATGACTGAGA AGTTCTTCCA CGCCATCATC AGTTCTTCCT CCGAGTTCCC CCCTCAGCTA 
CGCAGTGTGT GCCACTGCCT GTACCAGGCA ACTTGCCACT CTCTACTGAA TAAAGCTACC 
GTAAAAGAAA AAAAGGAAAA CAAAAAATCA GTTGTTAGCC AGAGATTCCC TCAGAACAGC 
ATTGGCGCCG TGGGGAGTGC CATGTTCCTG AGATTCATCA ACCCTGCCGT TGTCTCTCCC 
TACGAGGCAG GGATTTTAGA GAGAAAACCG CTCCCTAGAA TTGAAAGGGG GTTGAAGCTG 
ATGTCAAAGA TTCTTCAGAG CATTGCCAAT CATGTCCTTT TCACAAAAGA AGAGTATATG 
AGGCCTTTTA ACGATTTTGT GAAAAGCAAC TTTGATGTAG CACGGAGATT TTTCCTTGAT 
ATCGCATCTG ATTGTCCTGT AAGTGATGCC ATAAACCACA GCCTTTCCTT CATCAGCGAT 
GGCAATGTCT TGGCTTTGCA CCGTCTGCTC TGGAACAACC AAGAGAAAAT CGGCCAATAT 
CTTTCAAGCA ACAGAGATCA CAAAGCTGTT GGAAGGCGAC CTTTCGATAA GATGGCAACA 
CTGCTTGCCT ACCTGGGACC TCCAGAGCAC AAGCCTGTGG CTGACACACA CTGGTCCAGC 
CTAAATCTTA CCAGCTCCAA GTTTGAGGAA TTCATGACAA GGCACCAGGT ACATGAAAAA 
GAGGAATTCA AGGCTCTGAA AACATTAAAT ATCTTCTATC AAGCAGGCAC TTCCAAAGCT 
GGAAATCCTG TTTTTTATTA TGTCACACGA AGGTTCAAGA CTGGCCAGAT CAACGGGGAT 
TTGCTTATAT ACCACGTCTT ACTAACTTTA AAGCCATATT ATGCAAAGCC ATATGAGATT 
GTAATGGACC TCACTCATGC CGGGCCTAGC AATCGCTTTA AGACAGACTT CCTCTCCAAG 
TGGTTTGTTG TTTTCCCGGG CTTTGCTTAC GAGAACGTCT CTTCGGTCTA CATCTACAAC 
TGTAATTCTT GGGTTCGGGA GTACACCAAA TACCATGAGA GACTGCTCAC GGGACTCAAA 
GGCAGCAAAA GACTCTTTTT CATAGACTCT CCCTCAAAAC TGGCTGAGCA TATCGATTAT 
GATCAGCAAA AACTGCCAGC CGCGACCTTG GCTCTGGATG AAGACCTCAA GGTGTACAAC 
AGTGCTCTCA AGCTGGCTCA CAAGGACACC AAGGTGACCC TCAAGGTTGG CTCTACTGCG 
ATCCAAGTGA CGTCATCTGA GCGAACTAAA GTCCTGGGAC AGACGGTTTT TCTGAATGAT 
ATTTACTATG CCTCTGAGAT CGAAGAAATC TGTCTTGTGG ATGAAAACCA GTTCACTCTG 
ACCATTTCCA ATCAGGGCAC TCCCCTCACC TTCATGCATC AAGAGTGTGA AGGCATTGTG 
GCGTCCATCA TTCACATCCG AACTCGATGG GAGCTGTCCC AGCCAGATTC TATCCCCCAG 
CACACTAAGA TCCGGCCAAA GGACGTTCCC GGAACACTTT TGAATATCGC ATTACTTAAC 
TTGGGTAGTT CGGACCCAAG TTTACGGTCA GCCGCCTATA ATCTTCTCTG TGCCTTAACC 
TGTACCTTTA ATTTAAAAAT TGAGGGCCAA TTACTAGAAA CATCAGGTCT GTGTATACCT 
GCCAACAATA CCCTTTTTAT TGTCTCTATT AGTAAAACCC TGGCAGCTAA TGAGCCACAC 
CTCACCTTAG AGTTTTTGGA AGAGTGCATT TCCGGATTTA GCAAATCCAG TATTGAACTG 
AAGCACCTGT GTCTAGAATA CATGACTCCC TGGCTATCAA ATCTAGTCCG TTTCTGTAAA 
CATAATGATG ATGCCAAACG ACAGAGAGTC ACTGCTATTC TTGATAAACT TATAACAATG 
ACCATAAATG AAAAGCAGAT GTACCCATCG ATCCAAGCCA AGATATGGGG AAGCCTTGGG 
CAGATTACAG ATCTGCTCGA TGTAGTGCTG GACAGCTTTA TCAAAACCAG TGCCACAGGT 
GGTTTAGGAT CCATCAAGGC TGAAGTGATG GCTGACACGG CCGTGGCTCT GGCTTCTGGG 
AACGTCAAAC TAGTTTCGAG CAAGGTGATT GGAAGAATGT GCAAAATCAT TGACAAGACC 
TGCCTCTCTC CAACTCCTAC CTTAGAGCAG CACCTCATGT GGGATGATAT CGCCATTTTA 
GCACGCTACA TGCTAATGCT GTCCTTCAAT AATTCTCTGG ATGTGGCGGC TCATCTTCCC 
TACCTCTTCC ATGTGGTGAC TCTGTTGGTG GCCACAGGTC CCTTGTCCCT TCGTGCTTCC 
ACACACGGAC TGGTCATCAA TATCATCCAT TCTCTGTGCA CGTGCTCACA GCTTCACTTT 
AGTGAAGAGA CCAAGCAAGT TTTACGACTG AGTCTGACTG AGTTCTCACT ACCTAAGTTT 
TATTTGCTGT TCGGCATCAG CAAAGTCAAA TCAGCTGCCG TGATAGCCTT CCGGTCCAGT 
TACCGGGACA GGTCTTTCTC CCCTGGCTCC TATGAGAGGG AGACCTTTGC CCTGGCCTCC 
TTGGAAACAG TCACAGAAGC TCTCTTGGAG ATCATGGAGG CTTGTATGAG AGATATTCCT 
GCATGCAAGT GGCTGGACCA GTGGACAGAG CTAGCCCAGA GGTTTGCGTT CCAGTACAAT 
CCCTCCCTGC AGCCACGAGC TCTGGTTGTG TTTGGTTGTA TCAGCAAAAG AGTGTCCCAT 
GGGCAGATCA AGCAGATTAT CCGCATTCTT AGCAAGGGAC TTGAGAGCTG CCTAAAAGGA 
CCTGATAATT ATAACAGTCA AGTTCTGATT GAAGCTACCG TGATAGCTCT AACCAAATTA 
CAACCACTTC TTAATAAGNN NNNCCCTCTG CACCGAGCTC TCTTCTGGGT GGCCGTTGCC 
GTGCTGCAGC TGGATGAAGT GGACCTGTAC TCGGCGGGCA CTGCCCTCCT CGAACAGAAC 
CTGCACACCT TAGACAGCCT CCTGCACATC TTCAACGACC AGAGCCCAGA AGAAGTGTTC 
ATGGAGATCC GGCAGCCTCT GGAGTGGCAC TGCAAGCAGA TGGACCACTT TGTGGGGCTC 
AACTTTAACT CCAGCTTTAA CTTTGCCCTG GTCGGGCACC TGCTGAAGGG ATTTCGGCAT 
CCTTCACCTG TCACTGTTGC AAGAACTGTG AGGATTTTGC ACACCCTACT AGCATTGGTT 
AACAAGCATA GGAATTGTGA CAAGTTTGAG GTGAACACCC AGAGTGTGGC TTACTTAGCA 
GCTTTACTTA CTGTGTCTGA GGAGGTTCGA AGTCGCTGCA GCTTAAAGCA TAGGAAATCA 
CTCCTACTAA CTGATATCAC AATGGAAAAT GTGCCTATGG ATATATATCC CATCTATCAC 
AGTGACCCCA GCCTTAGGAC ACTAAAAGAA ACACAACCGT GGTCCTCTCC AAAGGGATCT 
GAGAGACATC TTGCAGCCAG CTACCCTCCT GTGGGCCAAA CTAGCCCCCG AACCAGAAAA 
TCCATGAGCC TGGATATGGG GCAGCCTTCC CAGGCCAACA CAAAGAAGTT GCTTGGGACA 
AGGAAAAGCT TCGATCATTT GATCTCTGAT GCCAAAGCTC CCAAAAGGCA AGAACAGGAG 
TCTGGAGTCA CCACGCCCCC CAAGATGCGG AGAGTGGCCG AGAACGACTA CGAGATGGAA 
ACCCAGAGAA TCTCCCCTCA GCCACACCCG CCTCTGCGGA AAGTGTCCGT GTCCGAATCC 
AATGTGCTGC TGGACGAGGA AGTGCTCACA GACCCCAAAA CCCAGGCTCT TCTTCTCACT 
GTCCTGGCTA CTCTGGTGAA GTACACTGCG GACGAGTTTG ACCAGCGCAT TCTCTATGAG 
TACTTAGCGG AGGCCAGTGT TGTCTTCCCA AAGGTTTTTC CAGTGGTACA TAATTTGTTG 
GACTCAAAGA TAAATACTCT GCTCTCATTA TGCCAAGACC CAAATTTGCT AAACCCGGTT 
CATGGAATTG TGCAGAGTGT GGTCTACCAC GAAGAGGCCC CGCCGCAGTA CCAGACGGCG 
TACCTCCAGA GTTTCGGTTT TAATGGTCTG TGGAGGTTTG CAGGACCATT TTCTAAGCAG 
ACGCAGATCC CAGACTATGC CGAGCTCATC GTGAAGTTTC TCGATGCCTT GATTGACACG 
TATCTGCCTG GAATTGATGA GGAAGCCAGT GAGGAGTCCC TCCTGACACC CACCTCTCCC 
TACCCGCCCG CGGTGCAGAG CCAGCTTAGC ATCACTGCGA ACCTCAACCT CTCTAACTCC 
ATGACCTCAC TGGCAACTTC CCAGCATTCC CCAGGCATCG ACAAGGAGAA TGTGGAGCTC 
TCGCCAACCA CCGGGCACTG CAGCAGGGTC ATTGGGGATC AAGGAAGCCA GCGTGGCCGA 
GAGGCCACTT TTTACGTCTC CCGTTTGGCC AAGGGCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967639.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823499.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X1, mRNA.

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CloneID	OSa234688
Clone ID Related Accession (Same CDS sequence)	XM_031967639.1
Accession Version	XM_031967639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGCAGTCA 
GATAGGAGTT CTTCCCATTT CCTCTACCTT TATGGGGCAG GCTATGATGT CTCTTCTGGT 
GGAGGTACCA GTCATATCTC TCTAGACCAC GAGGAGTTTA TGCGTACCTG TGCTCATGGA 
CCATCTCTCA GGAAAGTGAA AGGAAGCTCC TCAATGGACG GTGCAATCGG GTCCAGCGGG 
ACGCCCATCT GCCGCCAGGC CCAGACTAAG CTGGAGGTTG CTCTGTACAT GTTTCTGTGG 
AGTCCCGACA TTGAAGCCGT GCTCGTGGCC ATGTCCTGCT TTCGACACCT GTGTGAGGAA 
GCCGACATCA GGTGTGGCAT AGATGAGATC TCGGTGCACC ATTTCTTGCC AAACTATAAC 
ACCTTCATGG AGTTCGCATC TGTCAGCAAC ATGATGTCGA CAGGAAGAGC AGCTCTTCAG 
AAAAGAGTGA TGGCCCTGCT CAGGCGCATC GAACACCCCA CCGCTGGAAA CACCGAGGCT 
TGGGAAGATA CTTACGCCAA ATGGGAGCAA GCTACAAAAT CAATCCTCAA TTATCCGAAA 
GCCAAAATTG AAGATGGCCA GGTTGCTGAG AGTCTTCACA AGACCATTGT GAAGAGAAGA 
ATGTCTCACG TGAGTGGAGG GGGCTCCATA GACTTGTGCG ACACTGACTC GCTACAGGAG 
TGGATCAACA TGACTGGGTT TCTCTGTGCC CTTGGTGGCG TGTGCCTACA GCAGCGGAGC 
AATGCCGGCT TTGCCACCTA CAGCCCTCCC ATGGGCCCTA TCAGTGAACG CAAGGGCTCT 
CTCATTTCCA TGGTGTCCAG CGATGGAAAC GCAGAGACGC CTGTCAGCAA GTTCATGGAT 
CGGCTGCTCT CCTTGATGGT GTGTAACCAC GAAAAAGTGG GGCTGCAGAT ACGAAGCAAT 
GTCAAGGACT TGTTGGGTCT TGAATTGAGT CCTGCCCTTT ATCCAATGCT GTTTAACAAA 
TTAAAAAATA CCATCAGCAA GTTTTTTGAT CCTCAAGGAC AGGTTTTATT GACTGACACC 
AACACCCAGT TTGTGGAGCA GACCATAGCC ATAATGAAGA ACTTGCTTGA TAATCATGCT 
GAGGGTAGTT CTGAACACAT GGGACAAGCT AGCATCGAAA CTATGATGTT AAATCTGGTC 
AGATATGTTA GAGTGTTTGG GAATTTGGTG CAGGCAATTC AGATCAAAAC TAAGCTGTGC 
CAGCTGGTTC AAGTAATGAT GGAGAGGAGA GATGACCTTT CATTTTGCCA AGAGATGAAA 
TTTAGGAATA AAATGGTAGA ATATCTGACA GATTGGGTCA TGGGAACATC TAACCAAGCT 
GCAGATGAAG ATGTGAAATG TCTAACTAGA GATTTGGACC AGGCAAGCAT GGAAGCAGTG 
GTGTCATTGC TCGCTGGGCT CCCTCTGCAG CCTGAGGAAG GCGATGGGGT GGAATTGATG 
GAAGCCAAAT CCCAGCTGTT CCTTAAGTAT TTCACCCTCT TTATGAACCT TCTTAATGAC 
TGCAGTGAGG TCGAAGAAGA AAATGTCCAG ATTGGGGGCA GGAAGCGCGG CATGTCGCGA 
AGGCTGGCGT CCCTGCGCCA CTGCACCGTG CTGGCAATGT CCAACCTGCT CAATGCGAAT 
GTAGACAGCG GCCTGATGCA CTCTATAGTG CTAGGATTGG GTTACCACAA AGATCTTCAG 
ACAAGAGCCA CCTTCATGGA AGTCCTCACA AAAATCCTTC AGCAAGGCAC AGAGTTTGAT 
ACGCTCGCAG AAACCGTGTT AGCTGATCGA TTTGAGAGAC TTGTTGAGTT GGTCACGATG 
ATGGGTGATC AAGGAGAGCT CCCCATAGCC ATGGCTCTGG CCAACGTGGT GCCTTGTTCT 
CAGTGGGATG AACTTGCTCG AGTCCTTGTT ACTCTCTTTG ATTCCCGACA CTTACTGTAT 
CAGCTCCTTT GGAACATGTT TTCTAAGGAA GTTGAGCTGG CAGACTCCAT GCAGACACTT 
TTTAGAGGAA ACAGCTTGGC CAGTAAAATC ATGACCTTCT GCTTTAAGGT CTACGGTGCT 
ACATACCTGC AGAAGCTTCT GGAGCCTTTA CTCCGCTTTG TGATCACATC CTCCGAATGG 
CAGCACGTCA GCTTCGAGGT CGATCCCACC AGGTTAGAGC ATACTGAGAG CCTCGAGGAC 
AACCAGCGGA ACCTTTTGCA AATGACTGAG AAGTTCTTCC ACGCCATCAT CAGTTCTTCC 
TCCGAGTTCC CCCCTCAGCT ACGCAGTGTG TGCCACTGCC TGTACCAGGC AACTTGCCAC 
TCTCTACTGA ATAAAGCTAC CGTAAAAGAA AAAAAGGAAA ACAAAAAATC AGTTGTTAGC 
CAGAGATTCC CTCAGAACAG CATTGGCGCC GTGGGGAGTG CCATGTTCCT GAGATTCATC 
AACCCTGCCG TTGTCTCTCC CTACGAGGCA GGGATTTTAG AGAGAAAACC GCTCCCTAGA 
ATTGAAAGGG GGTTGAAGCT GATGTCAAAG ATTCTTCAGA GCATTGCCAA TCATGTCCTT 
TTCACAAAAG AAGAGTATAT GAGGCCTTTT AACGATTTTG TGAAAAGCAA CTTTGATGTA 
GCACGGAGAT TTTTCCTTGA TATCGCATCT GATTGTCCTG TAAGTGATGC CATAAACCAC 
AGCCTTTCCT TCATCAGCGA TGGCAATGTC TTGGCTTTGC ACCGTCTGCT CTGGAACAAC 
CAAGAGAAAA TCGGCCAATA TCTTTCAAGC AACAGAGATC ACAAAGCTGT TGGAAGGCGA 
CCTTTCGATA AGATGGCAAC ACTGCTTGCC TACCTGGGAC CTCCAGAGCA CAAGCCTGTG 
GCTGACACAC ACTGGTCCAG CCTAAATCTT ACCAGCTCCA AGTTTGAGGA ATTCATGACA 
AGGCACCAGG TACATGAAAA AGAGGAATTC AAGGCTCTGA AAACATTAAA TATCTTCTAT 
CAAGCAGGCA CTTCCAAAGC TGGAAATCCT GTTTTTTATT ATGTCACACG AAGGTTCAAG 
ACTGGCCAGA TCAACGGGGA TTTGCTTATA TACCACGTCT TACTAACTTT AAAGCCATAT 
TATGCAAAGC CATATGAGAT TGTAATGGAC CTCACTCATG CCGGGCCTAG CAATCGCTTT 
AAGACAGACT TCCTCTCCAA GTGGTTTGTT GTTTTCCCGG GCTTTGCTTA CGAGAACGTC 
TCTTCGGTCT ACATCTACAA CTGTAATTCT TGGGTTCGGG AGTACACCAA ATACCATGAG 
AGACTGCTCA CGGGACTCAA AGGCAGCAAA AGACTCTTTT TCATAGACTC TCCCTCAAAA 
CTGGCTGAGC ATATCGATTA TGATCAGCAA AAACTGCCAG CCGCGACCTT GGCTCTGGAT 
GAAGACCTCA AGGTGTACAA CAGTGCTCTC AAGCTGGCTC ACAAGGACAC CAAGGTGACC 
CTCAAGGTTG GCTCTACTGC GATCCAAGTG ACGTCATCTG AGCGAACTAA AGTCCTGGGA 
CAGACGGTTT TTCTGAATGA TATTTACTAT GCCTCTGAGA TCGAAGAAAT CTGTCTTGTG 
GATGAAAACC AGTTCACTCT GACCATTTCC AATCAGGGCA CTCCCCTCAC CTTCATGCAT 
CAAGAGTGTG AAGGCATTGT GGCGTCCATC ATTCACATCC GAACTCGATG GGAGCTGTCC 
CAGCCAGATT CTATCCCCCA GCACACTAAG ATCCGGCCAA AGGACGTTCC CGGAACACTT 
TTGAATATCG CATTACTTAA CTTGGGTAGT TCGGACCCAA GTTTACGGTC AGCCGCCTAT 
AATCTTCTCT GTGCCTTAAC CTGTACCTTT AATTTAAAAA TTGAGGGCCA ATTACTAGAA 
ACATCAGGTC TGTGTATACC TGCCAACAAT ACCCTTTTTA TTGTCTCTAT TAGTAAAACC 
CTGGCAGCTA ATGAGCCACA CCTCACCTTA GAGTTTTTGG AAGAGTGCAT TTCCGGATTT 
AGCAAATCCA GTATTGAACT GAAGCACCTG TGTCTAGAAT ACATGACTCC CTGGCTATCA 
AATCTAGTCC GTTTCTGTAA ACATAATGAT GATGCCAAAC GACAGAGAGT CACTGCTATT 
CTTGATAAAC TTATAACAAT GACCATAAAT GAAAAGCAGA TGTACCCATC GATCCAAGCC 
AAGATATGGG GAAGCCTTGG GCAGATTACA GATCTGCTCG ATGTAGTGCT GGACAGCTTT 
ATCAAAACCA GTGCCACAGG TGGTTTAGGA TCCATCAAGG CTGAAGTGAT GGCTGACACG 
GCCGTGGCTC TGGCTTCTGG GAACGTCAAA CTAGTTTCGA GCAAGGTGAT TGGAAGAATG 
TGCAAAATCA TTGACAAGAC CTGCCTCTCT CCAACTCCTA CCTTAGAGCA GCACCTCATG 
TGGGATGATA TCGCCATTTT AGCACGCTAC ATGCTAATGC TGTCCTTCAA TAATTCTCTG 
GATGTGGCGG CTCATCTTCC CTACCTCTTC CATGTGGTGA CTCTGTTGGT GGCCACAGGT 
CCCTTGTCCC TTCGTGCTTC CACACACGGA CTGGTCATCA ATATCATCCA TTCTCTGTGC 
ACGTGCTCAC AGCTTCACTT TAGTGAAGAG ACCAAGCAAG TTTTACGACT GAGTCTGACT 
GAGTTCTCAC TACCTAAGTT TTATTTGCTG TTCGGCATCA GCAAAGTCAA ATCAGCTGCC 
GTGATAGCCT TCCGGTCCAG TTACCGGGAC AGGTCTTTCT CCCCTGGCTC CTATGAGAGG 
GAGACCTTTG CCCTGGCCTC CTTGGAAACA GTCACAGAAG CTCTCTTGGA GATCATGGAG 
GCTTGTATGA GAGATATTCC TGCATGCAAG TGGCTGGACC AGTGGACAGA GCTAGCCCAG 
AGGTTTGCGT TCCAGTACAA TCCCTCCCTG CAGCCACGAG CTCTGGTTGT GTTTGGTTGT 
ATCAGCAAAA GAGTGTCCCA TGGGCAGATC AAGCAGATTA TCCGCATTCT TAGCAAGGGA 
CTTGAGAGCT GCCTAAAAGG ACCTGATAAT TATAACAGTC AAGTTCTGAT TGAAGCTACC 
GTGATAGCTC TAACCAAATT ACAACCACTT CTTAATAAGC AGGAGTCCCC TCTGCACCGA 
GCTCTCTTCT GGGTGGCCGT TGCCGTGCTG CAGCTGGATG AAGTGGACCT GTACTCGGCG 
GGCACTGCCC TCCTCGAACA GAACCTGCAC ACCTTAGACA GCCTCCTGCA CATCTTCAAC 
GACCAGAGCC CAGAAGAAGT GTTCATGGAG ATCCGGCAGC CTCTGGAGTG GCACTGCAAG 
CAGATGGACC ACTTTGTGGG GCTCAACTTT AACTCCAGCT TTAACTTTGC CCTGGTCGGG 
CACCTGCTGA AGGGATTTCG GCATCCTTCA CCTGTCACTG TTGCAAGAAC TGTGAGGATT 
TTGCACACCC TACTAGCATT GGTTAACAAG CATAGGAATT GTGACAAGTT TGAGGTGAAC 
ACCCAGAGTG TGGCTTACTT AGCAGCTTTA CTTACTGTGT CTGAGGAGGT TCGAAGTCGC 
TGCAGCTTAA AGCATAGGAA ATCACTCCTA CTAACTGATA TCACAATGGA AAATGTGCCT 
ATGGATATAT ATCCCATCTA TCACAGTGAC CCCAGCCTTA GGACACTAAA AGAAACACAA 
CCGTGGTCCT CTCCAAAGGG ATCTGAGAGA CATCTTGCAG CCAGCTACCC TCCTGTGGGC 
CAAACTAGCC CCCGAACCAG AAAATCCATG AGCCTGGATA TGGGGCAGCC TTCCCAGGCC 
AACACAAAGA AGTTGCTTGG GACAAGGAAA AGCTTCGATC ATTTGATCTC TGATGCCAAA 
GCTCCCAAAA GGCAAGAACA GGAGTCTGGA GTCACCACGC CCCCCAAGAT GCGGAGAGTG 
GCCGAGAACG ACTACGAGAT GGAAACCCAG AGAATCTCCC CTCAGCCACA CCCGCCTCTG 
CGGAAAGTGT CCGTGTCCGA ATCCAATGTG CTGCTGGACG AGGAAGTGCT CACAGACCCC 
AAAACCCAGG CTCTTCTTCT CACTGTCCTG GCTACTCTGG TGAAGTACAC TGCGGACGAG 
TTTGACCAGC GCATTCTCTA TGAGTACTTA GCGGAGGCCA GTGTTGTCTT CCCAAAGGTT 
TTTCCAGTGG TACATAATTT GTTGGACTCA AAGATAAATA CTCTGCTCTC ATTATGCCAA 
GACCCAAATT TGCTAAACCC GGTTCATGGA ATTGTGCAGA GTGTGGTCTA CCACGAAGAG 
GCCCCGCCGC AGTACCAGAC GGCGTACCTC CAGAGTTTCG GTTTTAATGG TCTGTGGAGG 
TTTGCAGGAC CATTTTCTAA GCAGACGCAG ATCCCAGACT ATGCCGAGCT CATCGTGAAG 
TTTCTCGATG CCTTGATTGA CACGTATCTG CCTGGAATTG ATGAGGAAGC CAGTGAGGAG 
TCCCTCCTGA CACCCACCTC TCCCTACCCG CCCGCGGTGC AGAGCCAGCT TAGCATCACT 
GCGAACCTCA ACCTCTCTAA CTCCATGACC TCACTGGCAA CTTCCCAGCA TTCCCCAGGC 
ATCGACAAGG AGAATGTGGA GCTCTCGCCA ACCACCGGGC ACTGCAGCGG CGGGAGGACG 
CGCCACGGCT CCGCCAGCCA GGTCCAGAAG CAAAGGAGTG CCGGCAGCTT CAAGAGGCAC 
AGCATCAAGA AGATCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823499.1
CDS	9..8669
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGVSDLQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKFSTLLTTITAWILSPWLCDTDVSIPLSCQITRPQSSFKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIVLGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKQESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967639.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967639.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGCAGTCA 
GATAGGAGTT CTTCCCATTT CCTCTACCTT TATGGGGCAG GCTATGATGT CTCTTCTGGT 
GGAGGTACCA GTCATATCTC TCTAGACCAC GAGGAGTTTA TGCGTACCTG TGCTCATGGA 
CCATCTCTCA GGAAAGTGAA AGGAAGCTCC TCAATGGACG GTGCAATCGG GTCCAGCGGG 
ACGCCCATCT GCCGCCAGGC CCAGACTAAG CTGGAGGTTG CTCTGTACAT GTTTCTGTGG 
AGTCCCGACA TTGAAGCCGT GCTCGTGGCC ATGTCCTGCT TTCGACACCT GTGTGAGGAA 
GCCGACATCA GGTGTGGCAT AGATGAGATC TCGGTGCACC ATTTCTTGCC AAACTATAAC 
ACCTTCATGG AGTTCGCATC TGTCAGCAAC ATGATGTCGA CAGGAAGAGC AGCTCTTCAG 
AAAAGAGTGA TGGCCCTGCT CAGGCGCATC GAACACCCCA CCGCTGGAAA CACCGAGGCT 
TGGGAAGATA CTTACGCCAA ATGGGAGCAA GCTACAAAAT CAATCCTCAA TTATCCGAAA 
GCCAAAATTG AAGATGGCCA GGTTGCTGAG AGTCTTCACA AGACCATTGT GAAGAGAAGA 
ATGTCTCACG TGAGTGGAGG GGGCTCCATA GACTTGTGCG ACACTGACTC GCTACAGGAG 
TGGATCAACA TGACTGGGTT TCTCTGTGCC CTTGGTGGCG TGTGCCTACA GCAGCGGAGC 
AATGCCGGCT TTGCCACCTA CAGCCCTCCC ATGGGCCCTA TCAGTGAACG CAAGGGCTCT 
CTCATTTCCA TGGTGTCCAG CGATGGAAAC GCAGAGACGC CTGTCAGCAA GTTCATGGAT 
CGGCTGCTCT CCTTGATGGT GTGTAACCAC GAAAAAGTGG GGCTGCAGAT ACGAAGCAAT 
GTCAAGGACT TGTTGGGTCT TGAATTGAGT CCTGCCCTTT ATCCAATGCT GTTTAACAAA 
TTAAAAAATA CCATCAGCAA GTTTTTTGAT CCTCAAGGAC AGGTTTTATT GACTGACACC 
AACACCCAGT TTGTGGAGCA GACCATAGCC ATAATGAAGA ACTTGCTTGA TAATCATGCT 
GAGGGTAGTT CTGAACACAT GGGACAAGCT AGCATCGAAA CTATGATGTT AAATCTGGTC 
AGATATGTTA GAGTGTTTGG GAATTTGGTG CAGGCAATTC AGATCAAAAC TAAGCTGTGC 
CAGCTGGTTC AAGTAATGAT GGAGAGGAGA GATGACCTTT CATTTTGCCA AGAGATGAAA 
TTTAGGAATA AAATGGTAGA ATATCTGACA GATTGGGTCA TGGGAACATC TAACCAAGCT 
GCAGATGAAG ATGTGAAATG TCTAACTAGA GATTTGGACC AGGCAAGCAT GGAAGCAGTG 
GTGTCATTGC TCGCTGGGCT CCCTCTGCAG CCTGAGGAAG GCGATGGGGT GGAATTGATG 
GAAGCCAAAT CCCAGCTGTT CCTTAAGTAT TTCACCCTCT TTATGAACCT TCTTAATGAC 
TGCAGTGAGG TCGAAGAAGA AAATGTCCAG ATTGGGGGCA GGAAGCGCGG CATGTCGCGA 
AGGCTGGCGT CCCTGCGCCA CTGCACCGTG CTGGCAATGT CCAACCTGCT CAATGCGAAT 
GTAGACAGCG GCCTGATGCA CTCTATAGTG CTAGGATTGG GTTACCACAA AGATCTTCAG 
ACAAGAGCCA CCTTCATGGA AGTCCTCACA AAAATCCTTC AGCAAGGCAC AGAGTTTGAT 
ACGCTCGCAG AAACCGTGTT AGCTGATCGA TTTGAGAGAC TTGTTGAGTT GGTCACGATG 
ATGGGTGATC AAGGAGAGCT CCCCATAGCC ATGGCTCTGG CCAACGTGGT GCCTTGTTCT 
CAGTGGGATG AACTTGCTCG AGTCCTTGTT ACTCTCTTTG ATTCCCGACA CTTACTGTAT 
CAGCTCCTTT GGAACATGTT TTCTAAGGAA GTTGAGCTGG CAGACTCCAT GCAGACACTT 
TTTAGAGGAA ACAGCTTGGC CAGTAAAATC ATGACCTTCT GCTTTAAGGT CTACGGTGCT 
ACATACCTGC AGAAGCTTCT GGAGCCTTTA CTCCGCTTTG TGATCACATC CTCCGAATGG 
CAGCACGTCA GCTTCGAGGT CGATCCCACC AGGTTAGAGC ATACTGAGAG CCTCGAGGAC 
AACCAGCGGA ACCTTTTGCA AATGACTGAG AAGTTCTTCC ACGCCATCAT CAGTTCTTCC 
TCCGAGTTCC CCCCTCAGCT ACGCAGTGTG TGCCACTGCC TGTACCAGGC AACTTGCCAC 
TCTCTACTGA ATAAAGCTAC CGTAAAAGAA AAAAAGGAAA ACAAAAAATC AGTTGTTAGC 
CAGAGATTCC CTCAGAACAG CATTGGCGCC GTGGGGAGTG CCATGTTCCT GAGATTCATC 
AACCCTGCCG TTGTCTCTCC CTACGAGGCA GGGATTTTAG AGAGAAAACC GCTCCCTAGA 
ATTGAAAGGG GGTTGAAGCT GATGTCAAAG ATTCTTCAGA GCATTGCCAA TCATGTCCTT 
TTCACAAAAG AAGAGTATAT GAGGCCTTTT AACGATTTTG TGAAAAGCAA CTTTGATGTA 
GCACGGAGAT TTTTCCTTGA TATCGCATCT GATTGTCCTG TAAGTGATGC CATAAACCAC 
AGCCTTTCCT TCATCAGCGA TGGCAATGTC TTGGCTTTGC ACCGTCTGCT CTGGAACAAC 
CAAGAGAAAA TCGGCCAATA TCTTTCAAGC AACAGAGATC ACAAAGCTGT TGGAAGGCGA 
CCTTTCGATA AGATGGCAAC ACTGCTTGCC TACCTGGGAC CTCCAGAGCA CAAGCCTGTG 
GCTGACACAC ACTGGTCCAG CCTAAATCTT ACCAGCTCCA AGTTTGAGGA ATTCATGACA 
AGGCACCAGG TACATGAAAA AGAGGAATTC AAGGCTCTGA AAACATTAAA TATCTTCTAT 
CAAGCAGGCA CTTCCAAAGC TGGAAATCCT GTTTTTTATT ATGTCACACG AAGGTTCAAG 
ACTGGCCAGA TCAACGGGGA TTTGCTTATA TACCACGTCT TACTAACTTT AAAGCCATAT 
TATGCAAAGC CATATGAGAT TGTAATGGAC CTCACTCATG CCGGGCCTAG CAATCGCTTT 
AAGACAGACT TCCTCTCCAA GTGGTTTGTT GTTTTCCCGG GCTTTGCTTA CGAGAACGTC 
TCTTCGGTCT ACATCTACAA CTGTAATTCT TGGGTTCGGG AGTACACCAA ATACCATGAG 
AGACTGCTCA CGGGACTCAA AGGCAGCAAA AGACTCTTTT TCATAGACTC TCCCTCAAAA 
CTGGCTGAGC ATATCGATTA TGATCAGCAA AAACTGCCAG CCGCGACCTT GGCTCTGGAT 
GAAGACCTCA AGGTGTACAA CAGTGCTCTC AAGCTGGCTC ACAAGGACAC CAAGGTGACC 
CTCAAGGTTG GCTCTACTGC GATCCAAGTG ACGTCATCTG AGCGAACTAA AGTCCTGGGA 
CAGACGGTTT TTCTGAATGA TATTTACTAT GCCTCTGAGA TCGAAGAAAT CTGTCTTGTG 
GATGAAAACC AGTTCACTCT GACCATTTCC AATCAGGGCA CTCCCCTCAC CTTCATGCAT 
CAAGAGTGTG AAGGCATTGT GGCGTCCATC ATTCACATCC GAACTCGATG GGAGCTGTCC 
CAGCCAGATT CTATCCCCCA GCACACTAAG ATCCGGCCAA AGGACGTTCC CGGAACACTT 
TTGAATATCG CATTACTTAA CTTGGGTAGT TCGGACCCAA GTTTACGGTC AGCCGCCTAT 
AATCTTCTCT GTGCCTTAAC CTGTACCTTT AATTTAAAAA TTGAGGGCCA ATTACTAGAA 
ACATCAGGTC TGTGTATACC TGCCAACAAT ACCCTTTTTA TTGTCTCTAT TAGTAAAACC 
CTGGCAGCTA ATGAGCCACA CCTCACCTTA GAGTTTTTGG AAGAGTGCAT TTCCGGATTT 
AGCAAATCCA GTATTGAACT GAAGCACCTG TGTCTAGAAT ACATGACTCC CTGGCTATCA 
AATCTAGTCC GTTTCTGTAA ACATAATGAT GATGCCAAAC GACAGAGAGT CACTGCTATT 
CTTGATAAAC TTATAACAAT GACCATAAAT GAAAAGCAGA TGTACCCATC GATCCAAGCC 
AAGATATGGG GAAGCCTTGG GCAGATTACA GATCTGCTCG ATGTAGTGCT GGACAGCTTT 
ATCAAAACCA GTGCCACAGG TGGTTTAGGA TCCATCAAGG CTGAAGTGAT GGCTGACACG 
GCCGTGGCTC TGGCTTCTGG GAACGTCAAA CTAGTTTCGA GCAAGGTGAT TGGAAGAATG 
TGCAAAATCA TTGACAAGAC CTGCCTCTCT CCAACTCCTA CCTTAGAGCA GCACCTCATG 
TGGGATGATA TCGCCATTTT AGCACGCTAC ATGCTAATGC TGTCCTTCAA TAATTCTCTG 
GATGTGGCGG CTCATCTTCC CTACCTCTTC CATGTGGTGA CTCTGTTGGT GGCCACAGGT 
CCCTTGTCCC TTCGTGCTTC CACACACGGA CTGGTCATCA ATATCATCCA TTCTCTGTGC 
ACGTGCTCAC AGCTTCACTT TAGTGAAGAG ACCAAGCAAG TTTTACGACT GAGTCTGACT 
GAGTTCTCAC TACCTAAGTT TTATTTGCTG TTCGGCATCA GCAAAGTCAA ATCAGCTGCC 
GTGATAGCCT TCCGGTCCAG TTACCGGGAC AGGTCTTTCT CCCCTGGCTC CTATGAGAGG 
GAGACCTTTG CCCTGGCCTC CTTGGAAACA GTCACAGAAG CTCTCTTGGA GATCATGGAG 
GCTTGTATGA GAGATATTCC TGCATGCAAG TGGCTGGACC AGTGGACAGA GCTAGCCCAG 
AGGTTTGCGT TCCAGTACAA TCCCTCCCTG CAGCCACGAG CTCTGGTTGT GTTTGGTTGT 
ATCAGCAAAA GAGTGTCCCA TGGGCAGATC AAGCAGATTA TCCGCATTCT TAGCAAGGGA 
CTTGAGAGCT GCCTAAAAGG ACCTGATAAT TATAACAGTC AAGTTCTGAT TGAAGCTACC 
GTGATAGCTC TAACCAAATT ACAACCACTT CTTAATAAGC AGGAGTCCCC TCTGCACCGA 
GCTCTCTTCT GGGTGGCCGT TGCCGTGCTG CAGCTGGATG AAGTGGACCT GTACTCGGCG 
GGCACTGCCC TCCTCGAACA GAACCTGCAC ACCTTAGACA GCCTCCTGCA CATCTTCAAC 
GACCAGAGCC CAGAAGAAGT GTTCATGGAG ATCCGGCAGC CTCTGGAGTG GCACTGCAAG 
CAGATGGACC ACTTTGTGGG GCTCAACTTT AACTCCAGCT TTAACTTTGC CCTGGTCGGG 
CACCTGCTGA AGGGATTTCG GCATCCTTCA CCTGTCACTG TTGCAAGAAC TGTGAGGATT 
TTGCACACCC TACTAGCATT GGTTAACAAG CATAGGAATT GTGACAAGTT TGAGGTGAAC 
ACCCAGAGTG TGGCTTACTT AGCAGCTTTA CTTACTGTGT CTGAGGAGGT TCGAAGTCGC 
TGCAGCTTAA AGCATAGGAA ATCACTCCTA CTAACTGATA TCACAATGGA AAATGTGCCT 
ATGGATATAT ATCCCATCTA TCACAGTGAC CCCAGCCTTA GGACACTAAA AGAAACACAA 
CCGTGGTCCT CTCCAAAGGG ATCTGAGAGA CATCTTGCAG CCAGCTACCC TCCTGTGGGC 
CAAACTAGCC CCCGAACCAG AAAATCCATG AGCCTGGATA TGGGGCAGCC TTCCCAGGCC 
AACACAAAGA AGTTGCTTGG GACAAGGAAA AGCTTCGATC ATTTGATCTC TGATGCCAAA 
GCTCCCAAAA GGCAAGAACA GGAGTCTGGA GTCACCACGC CCCCCAAGAT GCGGAGAGTG 
GCCGAGAACG ACTACGAGAT GGAAACCCAG AGAATCTCCC CTCAGCCACA CCCGCCTCTG 
CGGAAAGTGT CCGTGTCCGA ATCCAATGTG CTGCTGGACG AGGAAGTGCT CACAGACCCC 
AAAACCCAGG CTCTTCTTCT CACTGTCCTG GCTACTCTGG TGAAGTACAC TGCGGACGAG 
TTTGACCAGC GCATTCTCTA TGAGTACTTA GCGGAGGCCA GTGTTGTCTT CCCAAAGGTT 
TTTCCAGTGG TACATAATTT GTTGGACTCA AAGATAAATA CTCTGCTCTC ATTATGCCAA 
GACCCAAATT TGCTAAACCC GGTTCATGGA ATTGTGCAGA GTGTGGTCTA CCACGAAGAG 
GCCCCGCCGC AGTACCAGAC GGCGTACCTC CAGAGTTTCG GTTTTAATGG TCTGTGGAGG 
TTTGCAGGAC CATTTTCTAA GCAGACGCAG ATCCCAGACT ATGCCGAGCT CATCGTGAAG 
TTTCTCGATG CCTTGATTGA CACGTATCTG CCTGGAATTG ATGAGGAAGC CAGTGAGGAG 
TCCCTCCTGA CACCCACCTC TCCCTACCCG CCCGCGGTGC AGAGCCAGCT TAGCATCACT 
GCGAACCTCA ACCTCTCTAA CTCCATGACC TCACTGGCAA CTTCCCAGCA TTCCCCAGGC 
ATCGACAAGG AGAATGTGGA GCTCTCGCCA ACCACCGGGC ACTGCAGCGG CGGGAGGACG 
CGCCACGGCT CCGCCAGCCA GGTCCAGAAG CAAAGGAGTG CCGGCAGCTT CAAGAGGCAC 
AGCATCAAGA AGATCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967640.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823500.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X2, mRNA.

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CloneID	OSa234689
Clone ID Related Accession (Same CDS sequence)	XM_031967640.1
Accession Version	XM_031967640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGCAGTCA 
GATAGGAGTT CTTCCCATTT CCTCTACCTT TATGGGGCAG GCTATGATGT CTCTTCTGGT 
GGAGGTACCA GTCATATCTC TCTAGACCAC GAGGAGTTTA TGCGTACCTG TGCTCATGGA 
CCATCTCTCA GGAAAGTGAA AGGAAGCTCC TCAATGGACG GTGCAATCGG GTCCAGCGGG 
ACGCCCATCT GCCGCCAGGC CCAGACTAAG CTGGAGGTTG CTCTGTACAT GTTTCTGTGG 
AGTCCCGACA TTGAAGCCGT GCTCGTGGCC ATGTCCTGCT TTCGACACCT GTGTGAGGAA 
GCCGACATCA GGTGTGGCAT AGATGAGATC TCGGTGCACC ATTTCTTGCC AAACTATAAC 
ACCTTCATGG AGTTCGCATC TGTCAGCAAC ATGATGTCGA CAGGAAGAGC AGCTCTTCAG 
AAAAGAGTGA TGGCCCTGCT CAGGCGCATC GAACACCCCA CCGCTGGAAA CACCGAGGCT 
TGGGAAGATA CTTACGCCAA ATGGGAGCAA GCTACAAAAT CAATCCTCAA TTATCCGAAA 
GCCAAAATTG AAGATGGCCA GGTTGCTGAG AGTCTTCACA AGACCATTGT GAAGAGAAGA 
ATGTCTCACG TGAGTGGAGG GGGCTCCATA GACTTGTGCG ACACTGACTC GCTACAGGAG 
TGGATCAACA TGACTGGGTT TCTCTGTGCC CTTGGTGGCG TGTGCCTACA GCAGCGGAGC 
AATGCCGGCT TTGCCACCTA CAGCCCTCCC ATGGGCCCTA TCAGTGAACG CAAGGGCTCT 
CTCATTTCCA TGGTGTCCAG CGATGGAAAC GCAGAGACGC CTGTCAGCAA GTTCATGGAT 
CGGCTGCTCT CCTTGATGGT GTGTAACCAC GAAAAAGTGG GGCTGCAGAT ACGAAGCAAT 
GTCAAGGACT TGTTGGGTCT TGAATTGAGT CCTGCCCTTT ATCCAATGCT GTTTAACAAA 
TTAAAAAATA CCATCAGCAA GTTTTTTGAT CCTCAAGGAC AGGTTTTATT GACTGACACC 
AACACCCAGT TTGTGGAGCA GACCATAGCC ATAATGAAGA ACTTGCTTGA TAATCATGCT 
GAGGGTAGTT CTGAACACAT GGGACAAGCT AGCATCGAAA CTATGATGTT AAATCTGGTC 
AGATATGTTA GAGTGTTTGG GAATTTGGTG CAGGCAATTC AGATCAAAAC TAAGCTGTGC 
CAGCTGGTTC AAGTAATGAT GGAGAGGAGA GATGACCTTT CATTTTGCCA AGAGATGAAA 
TTTAGGAATA AAATGGTAGA ATATCTGACA GATTGGGTCA TGGGAACATC TAACCAAGCT 
GCAGATGAAG ATGTGAAATG TCTAACTAGA GATTTGGACC AGGCAAGCAT GGAAGCAGTG 
GTGTCATTGC TCGCTGGGCT CCCTCTGCAG CCTGAGGAAG GCGATGGGGT GGAATTGATG 
GAAGCCAAAT CCCAGCTGTT CCTTAAGTAT TTCACCCTCT TTATGAACCT TCTTAATGAC 
TGCAGTGAGG TCGAAGAAGA AAATGTCCAG ATTGGGGGCA GGAAGCGCGG CATGTCGCGA 
AGGCTGGCGT CCCTGCGCCA CTGCACCGTG CTGGCAATGT CCAACCTGCT CAATGCGAAT 
GTAGACAGCG GCCTGATGCA CTCTATAGTG CTAGGATTGG GTTACCACAA AGATCTTCAG 
ACAAGAGCCA CCTTCATGGA AGTCCTCACA AAAATCCTTC AGCAAGGCAC AGAGTTTGAT 
ACGCTCGCAG AAACCGTGTT AGCTGATCGA TTTGAGAGAC TTGTTGAGTT GGTCACGATG 
ATGGGTGATC AAGGAGAGCT CCCCATAGCC ATGGCTCTGG CCAACGTGGT GCCTTGTTCT 
CAGTGGGATG AACTTGCTCG AGTCCTTGTT ACTCTCTTTG ATTCCCGACA CTTACTGTAT 
CAGCTCCTTT GGAACATGTT TTCTAAGGAA GTTGAGCTGG CAGACTCCAT GCAGACACTT 
TTTAGAGGAA ACAGCTTGGC CAGTAAAATC ATGACCTTCT GCTTTAAGGT CTACGGTGCT 
ACATACCTGC AGAAGCTTCT GGAGCCTTTA CTCCGCTTTG TGATCACATC CTCCGAATGG 
CAGCACGTCA GCTTCGAGGT CGATCCCACC AGGTTAGAGC ATACTGAGAG CCTCGAGGAC 
AACCAGCGGA ACCTTTTGCA AATGACTGAG AAGTTCTTCC ACGCCATCAT CAGTTCTTCC 
TCCGAGTTCC CCCCTCAGCT ACGCAGTGTG TGCCACTGCC TGTACCAGGC AACTTGCCAC 
TCTCTACTGA ATAAAGCTAC CGTAAAAGAA AAAAAGGAAA ACAAAAAATC AGTTGTTAGC 
CAGAGATTCC CTCAGAACAG CATTGGCGCC GTGGGGAGTG CCATGTTCCT GAGATTCATC 
AACCCTGCCG TTGTCTCTCC CTACGAGGCA GGGATTTTAG AGAGAAAACC GCTCCCTAGA 
ATTGAAAGGG GGTTGAAGCT GATGTCAAAG ATTCTTCAGA GCATTGCCAA TCATGTCCTT 
TTCACAAAAG AAGAGTATAT GAGGCCTTTT AACGATTTTG TGAAAAGCAA CTTTGATGTA 
GCACGGAGAT TTTTCCTTGA TATCGCATCT GATTGTCCTG TAAGTGATGC CATAAACCAC 
AGCCTTTCCT TCATCAGCGA TGGCAATGTC TTGGCTTTGC ACCGTCTGCT CTGGAACAAC 
CAAGAGAAAA TCGGCCAATA TCTTTCAAGC AACAGAGATC ACAAAGCTGT TGGAAGGCGA 
CCTTTCGATA AGATGGCAAC ACTGCTTGCC TACCTGGGAC CTCCAGAGCA CAAGCCTGTG 
GCTGACACAC ACTGGTCCAG CCTAAATCTT ACCAGCTCCA AGTTTGAGGA ATTCATGACA 
AGGCACCAGG TACATGAAAA AGAGGAATTC AAGGCTCTGA AAACATTAAA TATCTTCTAT 
CAAGCAGGCA CTTCCAAAGC TGGAAATCCT GTTTTTTATT ATGTCACACG AAGGTTCAAG 
ACTGGCCAGA TCAACGGGGA TTTGCTTATA TACCACGTCT TACTAACTTT AAAGCCATAT 
TATGCAAAGC CATATGAGAT TGTAATGGAC CTCACTCATG CCGGGCCTAG CAATCGCTTT 
AAGACAGACT TCCTCTCCAA GTGGTTTGTT GTTTTCCCGG GCTTTGCTTA CGAGAACGTC 
TCTTCGGTCT ACATCTACAA CTGTAATTCT TGGGTTCGGG AGTACACCAA ATACCATGAG 
AGACTGCTCA CGGGACTCAA AGGCAGCAAA AGACTCTTTT TCATAGACTC TCCCTCAAAA 
CTGGCTGAGC ATATCGATTA TGATCAGCAA AAACTGCCAG CCGCGACCTT GGCTCTGGAT 
GAAGACCTCA AGGTGTACAA CAGTGCTCTC AAGCTGGCTC ACAAGGACAC CAAGGTGACC 
CTCAAGGTTG GCTCTACTGC GATCCAAGTG ACGTCATCTG AGCGAACTAA AGTCCTGGGA 
CAGACGGTTT TTCTGAATGA TATTTACTAT GCCTCTGAGA TCGAAGAAAT CTGTCTTGTG 
GATGAAAACC AGTTCACTCT GACCATTTCC AATCAGGGCA CTCCCCTCAC CTTCATGCAT 
CAAGAGTGTG AAGGCATTGT GGCGTCCATC ATTCACATCC GAACTCGATG GGAGCTGTCC 
CAGCCAGATT CTATCCCCCA GCACACTAAG ATCCGGCCAA AGGACGTTCC CGGAACACTT 
TTGAATATCG CATTACTTAA CTTGGGTAGT TCGGACCCAA GTTTACGGTC AGCCGCCTAT 
AATCTTCTCT GTGCCTTAAC CTGTACCTTT AATTTAAAAA TTGAGGGCCA ATTACTAGAA 
ACATCAGGTC TGTGTATACC TGCCAACAAT ACCCTTTTTA TTGTCTCTAT TAGTAAAACC 
CTGGCAGCTA ATGAGCCACA CCTCACCTTA GAGTTTTTGG AAGAGTGCAT TTCCGGATTT 
AGCAAATCCA GTATTGAACT GAAGCACCTG TGTCTAGAAT ACATGACTCC CTGGCTATCA 
AATCTAGTCC GTTTCTGTAA ACATAATGAT GATGCCAAAC GACAGAGAGT CACTGCTATT 
CTTGATAAAC TTATAACAAT GACCATAAAT GAAAAGCAGA TGTACCCATC GATCCAAGCC 
AAGATATGGG GAAGCCTTGG GCAGATTACA GATCTGCTCG ATGTAGTGCT GGACAGCTTT 
ATCAAAACCA GTGCCACAGG TGGTTTAGGA TCCATCAAGG CTGAAGTGAT GGCTGACACG 
GCCGTGGCTC TGGCTTCTGG GAACGTCAAA CTAGTTTCGA GCAAGGTGAT TGGAAGAATG 
TGCAAAATCA TTGACAAGAC CTGCCTCTCT CCAACTCCTA CCTTAGAGCA GCACCTCATG 
TGGGATGATA TCGCCATTTT AGCACGCTAC ATGCTAATGC TGTCCTTCAA TAATTCTCTG 
GATGTGGCGG CTCATCTTCC CTACCTCTTC CATGTGGTGA CTCTGTTGGT GGCCACAGGT 
CCCTTGTCCC TTCGTGCTTC CACACACGGA CTGGTCATCA ATATCATCCA TTCTCTGTGC 
ACGTGCTCAC AGCTTCACTT TAGTGAAGAG ACCAAGCAAG TTTTACGACT GAGTCTGACT 
GAGTTCTCAC TACCTAAGTT TTATTTGCTG TTCGGCATCA GCAAAGTCAA ATCAGCTGCC 
GTGATAGCCT TCCGGTCCAG TTACCGGGAC AGGTCTTTCT CCCCTGGCTC CTATGAGAGG 
GAGACCTTTG CCCTGGCCTC CTTGGAAACA GTCACAGAAG CTCTCTTGGA GATCATGGAG 
GCTTGTATGA GAGATATTCC TGCATGCAAG TGGCTGGACC AGTGGACAGA GCTAGCCCAG 
AGGTTTGCGT TCCAGTACAA TCCCTCCCTG CAGCCACGAG CTCTGGTTGT GTTTGGTTGT 
ATCAGCAAAA GAGTGTCCCA TGGGCAGATC AAGCAGATTA TCCGCATTCT TAGCAAGGGA 
CTTGAGAGCT GCCTAAAAGG ACCTGATAAT TATAACAGTC AAGTTCTGAT TGAAGCTACC 
GTGATAGCTC TAACCAAATT ACAACCACTT CTTAATAAGG AGTCCCCTCT GCACCGAGCT 
CTCTTCTGGG TGGCCGTTGC CGTGCTGCAG CTGGATGAAG TGGACCTGTA CTCGGCGGGC 
ACTGCCCTCC TCGAACAGAA CCTGCACACC TTAGACAGCC TCCTGCACAT CTTCAACGAC 
CAGAGCCCAG AAGAAGTGTT CATGGAGATC CGGCAGCCTC TGGAGTGGCA CTGCAAGCAG 
ATGGACCACT TTGTGGGGCT CAACTTTAAC TCCAGCTTTA ACTTTGCCCT GGTCGGGCAC 
CTGCTGAAGG GATTTCGGCA TCCTTCACCT GTCACTGTTG CAAGAACTGT GAGGATTTTG 
CACACCCTAC TAGCATTGGT TAACAAGCAT AGGAATTGTG ACAAGTTTGA GGTGAACACC 
CAGAGTGTGG CTTACTTAGC AGCTTTACTT ACTGTGTCTG AGGAGGTTCG AAGTCGCTGC 
AGCTTAAAGC ATAGGAAATC ACTCCTACTA ACTGATATCA CAATGGAAAA TGTGCCTATG 
GATATATATC CCATCTATCA CAGTGACCCC AGCCTTAGGA CACTAAAAGA AACACAACCG 
TGGTCCTCTC CAAAGGGATC TGAGAGACAT CTTGCAGCCA GCTACCCTCC TGTGGGCCAA 
ACTAGCCCCC GAACCAGAAA ATCCATGAGC CTGGATATGG GGCAGCCTTC CCAGGCCAAC 
ACAAAGAAGT TGCTTGGGAC AAGGAAAAGC TTCGATCATT TGATCTCTGA TGCCAAAGCT 
CCCAAAAGGC AAGAACAGGA GTCTGGAGTC ACCACGCCCC CCAAGATGCG GAGAGTGGCC 
GAGAACGACT ACGAGATGGA AACCCAGAGA ATCTCCCCTC AGCCACACCC GCCTCTGCGG 
AAAGTGTCCG TGTCCGAATC CAATGTGCTG CTGGACGAGG AAGTGCTCAC AGACCCCAAA 
ACCCAGGCTC TTCTTCTCAC TGTCCTGGCT ACTCTGGTGA AGTACACTGC GGACGAGTTT 
GACCAGCGCA TTCTCTATGA GTACTTAGCG GAGGCCAGTG TTGTCTTCCC AAAGGTTTTT 
CCAGTGGTAC ATAATTTGTT GGACTCAAAG ATAAATACTC TGCTCTCATT ATGCCAAGAC 
CCAAATTTGC TAAACCCGGT TCATGGAATT GTGCAGAGTG TGGTCTACCA CGAAGAGGCC 
CCGCCGCAGT ACCAGACGGC GTACCTCCAG AGTTTCGGTT TTAATGGTCT GTGGAGGTTT 
GCAGGACCAT TTTCTAAGCA GACGCAGATC CCAGACTATG CCGAGCTCAT CGTGAAGTTT 
CTCGATGCCT TGATTGACAC GTATCTGCCT GGAATTGATG AGGAAGCCAG TGAGGAGTCC 
CTCCTGACAC CCACCTCTCC CTACCCGCCC GCGGTGCAGA GCCAGCTTAG CATCACTGCG 
AACCTCAACC TCTCTAACTC CATGACCTCA CTGGCAACTT CCCAGCATTC CCCAGGCATC 
GACAAGGAGA ATGTGGAGCT CTCGCCAACC ACCGGGCACT GCAGCGGCGG GAGGACGCGC 
CACGGCTCCG CCAGCCAGGT CCAGAAGCAA AGGAGTGCCG GCAGCTTCAA GAGGCACAGC 
ATCAAGAAGA TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823500.1
CDS	9..8666
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGVSDLQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKFSTLLTTITAWILSPWLCDTDVSIPLSCQITRPQSSFKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIVLGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967640.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967640.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGCAGTCA 
GATAGGAGTT CTTCCCATTT CCTCTACCTT TATGGGGCAG GCTATGATGT CTCTTCTGGT 
GGAGGTACCA GTCATATCTC TCTAGACCAC GAGGAGTTTA TGCGTACCTG TGCTCATGGA 
CCATCTCTCA GGAAAGTGAA AGGAAGCTCC TCAATGGACG GTGCAATCGG GTCCAGCGGG 
ACGCCCATCT GCCGCCAGGC CCAGACTAAG CTGGAGGTTG CTCTGTACAT GTTTCTGTGG 
AGTCCCGACA TTGAAGCCGT GCTCGTGGCC ATGTCCTGCT TTCGACACCT GTGTGAGGAA 
GCCGACATCA GGTGTGGCAT AGATGAGATC TCGGTGCACC ATTTCTTGCC AAACTATAAC 
ACCTTCATGG AGTTCGCATC TGTCAGCAAC ATGATGTCGA CAGGAAGAGC AGCTCTTCAG 
AAAAGAGTGA TGGCCCTGCT CAGGCGCATC GAACACCCCA CCGCTGGAAA CACCGAGGCT 
TGGGAAGATA CTTACGCCAA ATGGGAGCAA GCTACAAAAT CAATCCTCAA TTATCCGAAA 
GCCAAAATTG AAGATGGCCA GGTTGCTGAG AGTCTTCACA AGACCATTGT GAAGAGAAGA 
ATGTCTCACG TGAGTGGAGG GGGCTCCATA GACTTGTGCG ACACTGACTC GCTACAGGAG 
TGGATCAACA TGACTGGGTT TCTCTGTGCC CTTGGTGGCG TGTGCCTACA GCAGCGGAGC 
AATGCCGGCT TTGCCACCTA CAGCCCTCCC ATGGGCCCTA TCAGTGAACG CAAGGGCTCT 
CTCATTTCCA TGGTGTCCAG CGATGGAAAC GCAGAGACGC CTGTCAGCAA GTTCATGGAT 
CGGCTGCTCT CCTTGATGGT GTGTAACCAC GAAAAAGTGG GGCTGCAGAT ACGAAGCAAT 
GTCAAGGACT TGTTGGGTCT TGAATTGAGT CCTGCCCTTT ATCCAATGCT GTTTAACAAA 
TTAAAAAATA CCATCAGCAA GTTTTTTGAT CCTCAAGGAC AGGTTTTATT GACTGACACC 
AACACCCAGT TTGTGGAGCA GACCATAGCC ATAATGAAGA ACTTGCTTGA TAATCATGCT 
GAGGGTAGTT CTGAACACAT GGGACAAGCT AGCATCGAAA CTATGATGTT AAATCTGGTC 
AGATATGTTA GAGTGTTTGG GAATTTGGTG CAGGCAATTC AGATCAAAAC TAAGCTGTGC 
CAGCTGGTTC AAGTAATGAT GGAGAGGAGA GATGACCTTT CATTTTGCCA AGAGATGAAA 
TTTAGGAATA AAATGGTAGA ATATCTGACA GATTGGGTCA TGGGAACATC TAACCAAGCT 
GCAGATGAAG ATGTGAAATG TCTAACTAGA GATTTGGACC AGGCAAGCAT GGAAGCAGTG 
GTGTCATTGC TCGCTGGGCT CCCTCTGCAG CCTGAGGAAG GCGATGGGGT GGAATTGATG 
GAAGCCAAAT CCCAGCTGTT CCTTAAGTAT TTCACCCTCT TTATGAACCT TCTTAATGAC 
TGCAGTGAGG TCGAAGAAGA AAATGTCCAG ATTGGGGGCA GGAAGCGCGG CATGTCGCGA 
AGGCTGGCGT CCCTGCGCCA CTGCACCGTG CTGGCAATGT CCAACCTGCT CAATGCGAAT 
GTAGACAGCG GCCTGATGCA CTCTATAGTG CTAGGATTGG GTTACCACAA AGATCTTCAG 
ACAAGAGCCA CCTTCATGGA AGTCCTCACA AAAATCCTTC AGCAAGGCAC AGAGTTTGAT 
ACGCTCGCAG AAACCGTGTT AGCTGATCGA TTTGAGAGAC TTGTTGAGTT GGTCACGATG 
ATGGGTGATC AAGGAGAGCT CCCCATAGCC ATGGCTCTGG CCAACGTGGT GCCTTGTTCT 
CAGTGGGATG AACTTGCTCG AGTCCTTGTT ACTCTCTTTG ATTCCCGACA CTTACTGTAT 
CAGCTCCTTT GGAACATGTT TTCTAAGGAA GTTGAGCTGG CAGACTCCAT GCAGACACTT 
TTTAGAGGAA ACAGCTTGGC CAGTAAAATC ATGACCTTCT GCTTTAAGGT CTACGGTGCT 
ACATACCTGC AGAAGCTTCT GGAGCCTTTA CTCCGCTTTG TGATCACATC CTCCGAATGG 
CAGCACGTCA GCTTCGAGGT CGATCCCACC AGGTTAGAGC ATACTGAGAG CCTCGAGGAC 
AACCAGCGGA ACCTTTTGCA AATGACTGAG AAGTTCTTCC ACGCCATCAT CAGTTCTTCC 
TCCGAGTTCC CCCCTCAGCT ACGCAGTGTG TGCCACTGCC TGTACCAGGC AACTTGCCAC 
TCTCTACTGA ATAAAGCTAC CGTAAAAGAA AAAAAGGAAA ACAAAAAATC AGTTGTTAGC 
CAGAGATTCC CTCAGAACAG CATTGGCGCC GTGGGGAGTG CCATGTTCCT GAGATTCATC 
AACCCTGCCG TTGTCTCTCC CTACGAGGCA GGGATTTTAG AGAGAAAACC GCTCCCTAGA 
ATTGAAAGGG GGTTGAAGCT GATGTCAAAG ATTCTTCAGA GCATTGCCAA TCATGTCCTT 
TTCACAAAAG AAGAGTATAT GAGGCCTTTT AACGATTTTG TGAAAAGCAA CTTTGATGTA 
GCACGGAGAT TTTTCCTTGA TATCGCATCT GATTGTCCTG TAAGTGATGC CATAAACCAC 
AGCCTTTCCT TCATCAGCGA TGGCAATGTC TTGGCTTTGC ACCGTCTGCT CTGGAACAAC 
CAAGAGAAAA TCGGCCAATA TCTTTCAAGC AACAGAGATC ACAAAGCTGT TGGAAGGCGA 
CCTTTCGATA AGATGGCAAC ACTGCTTGCC TACCTGGGAC CTCCAGAGCA CAAGCCTGTG 
GCTGACACAC ACTGGTCCAG CCTAAATCTT ACCAGCTCCA AGTTTGAGGA ATTCATGACA 
AGGCACCAGG TACATGAAAA AGAGGAATTC AAGGCTCTGA AAACATTAAA TATCTTCTAT 
CAAGCAGGCA CTTCCAAAGC TGGAAATCCT GTTTTTTATT ATGTCACACG AAGGTTCAAG 
ACTGGCCAGA TCAACGGGGA TTTGCTTATA TACCACGTCT TACTAACTTT AAAGCCATAT 
TATGCAAAGC CATATGAGAT TGTAATGGAC CTCACTCATG CCGGGCCTAG CAATCGCTTT 
AAGACAGACT TCCTCTCCAA GTGGTTTGTT GTTTTCCCGG GCTTTGCTTA CGAGAACGTC 
TCTTCGGTCT ACATCTACAA CTGTAATTCT TGGGTTCGGG AGTACACCAA ATACCATGAG 
AGACTGCTCA CGGGACTCAA AGGCAGCAAA AGACTCTTTT TCATAGACTC TCCCTCAAAA 
CTGGCTGAGC ATATCGATTA TGATCAGCAA AAACTGCCAG CCGCGACCTT GGCTCTGGAT 
GAAGACCTCA AGGTGTACAA CAGTGCTCTC AAGCTGGCTC ACAAGGACAC CAAGGTGACC 
CTCAAGGTTG GCTCTACTGC GATCCAAGTG ACGTCATCTG AGCGAACTAA AGTCCTGGGA 
CAGACGGTTT TTCTGAATGA TATTTACTAT GCCTCTGAGA TCGAAGAAAT CTGTCTTGTG 
GATGAAAACC AGTTCACTCT GACCATTTCC AATCAGGGCA CTCCCCTCAC CTTCATGCAT 
CAAGAGTGTG AAGGCATTGT GGCGTCCATC ATTCACATCC GAACTCGATG GGAGCTGTCC 
CAGCCAGATT CTATCCCCCA GCACACTAAG ATCCGGCCAA AGGACGTTCC CGGAACACTT 
TTGAATATCG CATTACTTAA CTTGGGTAGT TCGGACCCAA GTTTACGGTC AGCCGCCTAT 
AATCTTCTCT GTGCCTTAAC CTGTACCTTT AATTTAAAAA TTGAGGGCCA ATTACTAGAA 
ACATCAGGTC TGTGTATACC TGCCAACAAT ACCCTTTTTA TTGTCTCTAT TAGTAAAACC 
CTGGCAGCTA ATGAGCCACA CCTCACCTTA GAGTTTTTGG AAGAGTGCAT TTCCGGATTT 
AGCAAATCCA GTATTGAACT GAAGCACCTG TGTCTAGAAT ACATGACTCC CTGGCTATCA 
AATCTAGTCC GTTTCTGTAA ACATAATGAT GATGCCAAAC GACAGAGAGT CACTGCTATT 
CTTGATAAAC TTATAACAAT GACCATAAAT GAAAAGCAGA TGTACCCATC GATCCAAGCC 
AAGATATGGG GAAGCCTTGG GCAGATTACA GATCTGCTCG ATGTAGTGCT GGACAGCTTT 
ATCAAAACCA GTGCCACAGG TGGTTTAGGA TCCATCAAGG CTGAAGTGAT GGCTGACACG 
GCCGTGGCTC TGGCTTCTGG GAACGTCAAA CTAGTTTCGA GCAAGGTGAT TGGAAGAATG 
TGCAAAATCA TTGACAAGAC CTGCCTCTCT CCAACTCCTA CCTTAGAGCA GCACCTCATG 
TGGGATGATA TCGCCATTTT AGCACGCTAC ATGCTAATGC TGTCCTTCAA TAATTCTCTG 
GATGTGGCGG CTCATCTTCC CTACCTCTTC CATGTGGTGA CTCTGTTGGT GGCCACAGGT 
CCCTTGTCCC TTCGTGCTTC CACACACGGA CTGGTCATCA ATATCATCCA TTCTCTGTGC 
ACGTGCTCAC AGCTTCACTT TAGTGAAGAG ACCAAGCAAG TTTTACGACT GAGTCTGACT 
GAGTTCTCAC TACCTAAGTT TTATTTGCTG TTCGGCATCA GCAAAGTCAA ATCAGCTGCC 
GTGATAGCCT TCCGGTCCAG TTACCGGGAC AGGTCTTTCT CCCCTGGCTC CTATGAGAGG 
GAGACCTTTG CCCTGGCCTC CTTGGAAACA GTCACAGAAG CTCTCTTGGA GATCATGGAG 
GCTTGTATGA GAGATATTCC TGCATGCAAG TGGCTGGACC AGTGGACAGA GCTAGCCCAG 
AGGTTTGCGT TCCAGTACAA TCCCTCCCTG CAGCCACGAG CTCTGGTTGT GTTTGGTTGT 
ATCAGCAAAA GAGTGTCCCA TGGGCAGATC AAGCAGATTA TCCGCATTCT TAGCAAGGGA 
CTTGAGAGCT GCCTAAAAGG ACCTGATAAT TATAACAGTC AAGTTCTGAT TGAAGCTACC 
GTGATAGCTC TAACCAAATT ACAACCACTT CTTAATAAGG AGTCCCCTCT GCACCGAGCT 
CTCTTCTGGG TGGCCGTTGC CGTGCTGCAG CTGGATGAAG TGGACCTGTA CTCGGCGGGC 
ACTGCCCTCC TCGAACAGAA CCTGCACACC TTAGACAGCC TCCTGCACAT CTTCAACGAC 
CAGAGCCCAG AAGAAGTGTT CATGGAGATC CGGCAGCCTC TGGAGTGGCA CTGCAAGCAG 
ATGGACCACT TTGTGGGGCT CAACTTTAAC TCCAGCTTTA ACTTTGCCCT GGTCGGGCAC 
CTGCTGAAGG GATTTCGGCA TCCTTCACCT GTCACTGTTG CAAGAACTGT GAGGATTTTG 
CACACCCTAC TAGCATTGGT TAACAAGCAT AGGAATTGTG ACAAGTTTGA GGTGAACACC 
CAGAGTGTGG CTTACTTAGC AGCTTTACTT ACTGTGTCTG AGGAGGTTCG AAGTCGCTGC 
AGCTTAAAGC ATAGGAAATC ACTCCTACTA ACTGATATCA CAATGGAAAA TGTGCCTATG 
GATATATATC CCATCTATCA CAGTGACCCC AGCCTTAGGA CACTAAAAGA AACACAACCG 
TGGTCCTCTC CAAAGGGATC TGAGAGACAT CTTGCAGCCA GCTACCCTCC TGTGGGCCAA 
ACTAGCCCCC GAACCAGAAA ATCCATGAGC CTGGATATGG GGCAGCCTTC CCAGGCCAAC 
ACAAAGAAGT TGCTTGGGAC AAGGAAAAGC TTCGATCATT TGATCTCTGA TGCCAAAGCT 
CCCAAAAGGC AAGAACAGGA GTCTGGAGTC ACCACGCCCC CCAAGATGCG GAGAGTGGCC 
GAGAACGACT ACGAGATGGA AACCCAGAGA ATCTCCCCTC AGCCACACCC GCCTCTGCGG 
AAAGTGTCCG TGTCCGAATC CAATGTGCTG CTGGACGAGG AAGTGCTCAC AGACCCCAAA 
ACCCAGGCTC TTCTTCTCAC TGTCCTGGCT ACTCTGGTGA AGTACACTGC GGACGAGTTT 
GACCAGCGCA TTCTCTATGA GTACTTAGCG GAGGCCAGTG TTGTCTTCCC AAAGGTTTTT 
CCAGTGGTAC ATAATTTGTT GGACTCAAAG ATAAATACTC TGCTCTCATT ATGCCAAGAC 
CCAAATTTGC TAAACCCGGT TCATGGAATT GTGCAGAGTG TGGTCTACCA CGAAGAGGCC 
CCGCCGCAGT ACCAGACGGC GTACCTCCAG AGTTTCGGTT TTAATGGTCT GTGGAGGTTT 
GCAGGACCAT TTTCTAAGCA GACGCAGATC CCAGACTATG CCGAGCTCAT CGTGAAGTTT 
CTCGATGCCT TGATTGACAC GTATCTGCCT GGAATTGATG AGGAAGCCAG TGAGGAGTCC 
CTCCTGACAC CCACCTCTCC CTACCCGCCC GCGGTGCAGA GCCAGCTTAG CATCACTGCG 
AACCTCAACC TCTCTAACTC CATGACCTCA CTGGCAACTT CCCAGCATTC CCCAGGCATC 
GACAAGGAGA ATGTGGAGCT CTCGCCAACC ACCGGGCACT GCAGCGGCGG GAGGACGCGC 
CACGGCTCCG CCAGCCAGGT CCAGAAGCAA AGGAGTGCCG GCAGCTTCAA GAGGCACAGC 
ATCAAGAAGA TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967641.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823501.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X3, mRNA.

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CloneID	OSa234690
Clone ID Related Accession (Same CDS sequence)	XM_031967641.1
Accession Version	XM_031967641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCCAA AGGACACGAT GAGGTTAGAT 
GAAACAATGC TGGTCAAACA GTTGCTGCCG GAAATCTGCC ATTTCCTTCA CACCTACCGG 
GAAGGGAACC AGCACGCAGC CGAGCTTCGG AGCTCCGCCT CTGGCGTCCT TTTCTCCCTC 
AGCTGTAATA ACTTCAATGC CGTCTTCAGC CGGATTTCCA CCAGGTTACA GGAATTGACT 
GTTTGCTCAG AAGATAATGT TGATGTCCAT GATATAGAAT TATTACAATA TATCAATGTG 
GATTGTGCAA AACTAAAGCG GCTCTTGCAG GAAACTGTAT TTAAGTTTAA AGCCTTAAAG 
AAGGTAGCTC AACTAGCAGT TATAAATAGC CTAGAAAAGG CATTTTGGAA TTGGGTAGAG 
AACTATCCTG ATGAATTTAC AAAGCTGTAC CAGACTCCCC AGACAGATAT GGCGGAATGT 
GCAGAAAAAT TATTTGACTT GGTGGATGGT TTTGCTGAAA GCACCAAACG TAAAGCTGCA 
GTTTGGCCAC TGCAAATCAT TCTTCTTGTC TTGTGTCCAG AAATAATCCA GGACATTTCC 
AAGGATGTGG TTGATGAAGC GAAAATGAAT AAGTTTTCTA CACTGTTGAC CACTATCACC 
GCCTGGATAC TTTCTCCTTG GCTTTGTGAT ACCGATGTCT CAATTCCTCT TTCCTGCCAA 
ATCACTCGTC CTCAGTCCTC CTTTAAAATG TTTCTCGACA ATCTCAGAAG AGCGCTGGCC 
GGCCACAGTG GGAGCAGGCA GCTGACAGAG AGTGCCGCGA TCGCTTGTGT TAAACTGTGC 
AAAGCCAGCA CCTACATCAA CTGGGAGGAT AACTCGGTCA TTTTCCTGCT CGTCCAATCC 
ATGGTGGTGG ATCTGAAGAA CTTGCTCTTT AATCCCAGTA AGCCCTTCTC CAGAGGCAGC 
CAGAATGCTG ATGTGGACCT CATGATAGAC TGCCTGGTTT CTTGCTTTCG CATCAGCCCT 
CACAACAACC AACACTTTAA GATCTGTTTG GCCCAGAACT CCCCATCTAC ATTTCATTAT 
GTGCTGGTAA ACTCCCTCCA TCGAATTATC ACCAATAGCA CTTTCAAGCA TGGACTTGGC 
ACTGCTTCCG CCCTGGATTG GTGGCCCAAG ATTGATGCTG TGTATTGCCA TTCTGTAGAA 
CTTCGAAACA TGTTTGGTGA AACGCTTCAC AAGGCCGTGC AGGGCTGTGG GCCTCACCCC 
GCGATACGAA TGGCGCCGAG TCTTACATTT AAAGAAAAAA TGACAAGCCT TAAATTTAAA 
GAAAAACCTA CGGACTTGGA AACAAGAAGT TATAAATACC TCTTATTATC CATGGTGAAA 
CTAATCCATG CAGATCCAAA GCTGTTGCTA TGTAATCCAA GAAAACAAGG GCCTGAAACT 
CAGGGGAGTA CAGCAGAGTT AATCACAGGC CTTGTGCAGC TCGTCCCACA GTCCAGCATG 
CCAGAAATAG CTCAAGAAGC AATGGAGGCT TTGCTGGTTC TTCATCAGTT GGATAGTATT 
GACTTATGGA ATCCAGATTC ACCTATAGAA ACATTCTGGG AGATTAGCTC ACAAATGCTT 
TTTTATATCT GTAAAAAGCT AACTACCCAT CAGATGCTTA GCAGTACAGA AATTCTCAAG 
TGGTTGCGAG AAATTTTGAT CTGTAGGAAC AAATATCTTC TTAAAAATAA GCAGTCAGAT 
AGGAGTTCTT CCCATTTCCT CTACCTTTAT GGGGCAGGCT ATGATGTCTC TTCTGGTGGA 
GGTACCAGTC ATATCTCTCT AGACCACGAG GAGTTTATGC GTACCTGTGC TCATGGACCA 
TCTCTCAGGA AAGTGAAAGG AAGCTCCTCA ATGGACGGTG CAATCGGGTC CAGCGGGACG 
CCCATCTGCC GCCAGGCCCA GACTAAGCTG GAGGTTGCTC TGTACATGTT TCTGTGGAGT 
CCCGACATTG AAGCCGTGCT CGTGGCCATG TCCTGCTTTC GACACCTGTG TGAGGAAGCC 
GACATCAGGT GTGGCATAGA TGAGATCTCG GTGCACCATT TCTTGCCAAA CTATAACACC 
TTCATGGAGT TCGCATCTGT CAGCAACATG ATGTCGACAG GAAGAGCAGC TCTTCAGAAA 
AGAGTGATGG CCCTGCTCAG GCGCATCGAA CACCCCACCG CTGGAAACAC CGAGGCTTGG 
GAAGATACTT ACGCCAAATG GGAGCAAGCT ACAAAATCAA TCCTCAATTA TCCGAAAGCC 
AAAATTGAAG ATGGCCAGGT TGCTGAGAGT CTTCACAAGA CCATTGTGAA GAGAAGAATG 
TCTCACGTGA GTGGAGGGGG CTCCATAGAC TTGTGCGACA CTGACTCGCT ACAGGAGTGG 
ATCAACATGA CTGGGTTTCT CTGTGCCCTT GGTGGCGTGT GCCTACAGCA GCGGAGCAAT 
GCCGGCTTTG CCACCTACAG CCCTCCCATG GGCCCTATCA GTGAACGCAA GGGCTCTCTC 
ATTTCCATGG TGTCCAGCGA TGGAAACGCA GAGACGCCTG TCAGCAAGTT CATGGATCGG 
CTGCTCTCCT TGATGGTGTG TAACCACGAA AAAGTGGGGC TGCAGATACG AAGCAATGTC 
AAGGACTTGT TGGGTCTTGA ATTGAGTCCT GCCCTTTATC CAATGCTGTT TAACAAATTA 
AAAAATACCA TCAGCAAGTT TTTTGATCCT CAAGGACAGG TTTTATTGAC TGACACCAAC 
ACCCAGTTTG TGGAGCAGAC CATAGCCATA ATGAAGAACT TGCTTGATAA TCATGCTGAG 
GGTAGTTCTG AACACATGGG ACAAGCTAGC ATCGAAACTA TGATGTTAAA TCTGGTCAGA 
TATGTTAGAG TGTTTGGGAA TTTGGTGCAG GCAATTCAGA TCAAAACTAA GCTGTGCCAG 
CTGGTTCAAG TAATGATGGA GAGGAGAGAT GACCTTTCAT TTTGCCAAGA GATGAAATTT 
AGGAATAAAA TGGTAGAATA TCTGACAGAT TGGGTCATGG GAACATCTAA CCAAGCTGCA 
GATGAAGATG TGAAATGTCT AACTAGAGAT TTGGACCAGG CAAGCATGGA AGCAGTGGTG 
TCATTGCTCG CTGGGCTCCC TCTGCAGCCT GAGGAAGGCG ATGGGGTGGA ATTGATGGAA 
GCCAAATCCC AGCTGTTCCT TAAGTATTTC ACCCTCTTTA TGAACCTTCT TAATGACTGC 
AGTGAGGTCG AAGAAGAAAA TGTCCAGATT GGGGGCAGGA AGCGCGGCAT GTCGCGAAGG 
CTGGCGTCCC TGCGCCACTG CACCGTGCTG GCAATGTCCA ACCTGCTCAA TGCGAATGTA 
GACAGCGGCC TGATGCACTC TATAGTGCTA GGATTGGGTT ACCACAAAGA TCTTCAGACA 
AGAGCCACCT TCATGGAAGT CCTCACAAAA ATCCTTCAGC AAGGCACAGA GTTTGATACG 
CTCGCAGAAA CCGTGTTAGC TGATCGATTT GAGAGACTTG TTGAGTTGGT CACGATGATG 
GGTGATCAAG GAGAGCTCCC CATAGCCATG GCTCTGGCCA ACGTGGTGCC TTGTTCTCAG 
TGGGATGAAC TTGCTCGAGT CCTTGTTACT CTCTTTGATT CCCGACACTT ACTGTATCAG 
CTCCTTTGGA ACATGTTTTC TAAGGAAGTT GAGCTGGCAG ACTCCATGCA GACACTTTTT 
AGAGGAAACA GCTTGGCCAG TAAAATCATG ACCTTCTGCT TTAAGGTCTA CGGTGCTACA 
TACCTGCAGA AGCTTCTGGA GCCTTTACTC CGCTTTGTGA TCACATCCTC CGAATGGCAG 
CACGTCAGCT TCGAGGTCGA TCCCACCAGG TTAGAGCATA CTGAGAGCCT CGAGGACAAC 
CAGCGGAACC TTTTGCAAAT GACTGAGAAG TTCTTCCACG CCATCATCAG TTCTTCCTCC 
GAGTTCCCCC CTCAGCTACG CAGTGTGTGC CACTGCCTGT ACCAGGCAAC TTGCCACTCT 
CTACTGAATA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTTAGCCAG 
AGATTCCCTC AGAACAGCAT TGGCGCCGTG GGGAGTGCCA TGTTCCTGAG ATTCATCAAC 
CCTGCCGTTG TCTCTCCCTA CGAGGCAGGG ATTTTAGAGA GAAAACCGCT CCCTAGAATT 
GAAAGGGGGT TGAAGCTGAT GTCAAAGATT CTTCAGAGCA TTGCCAATCA TGTCCTTTTC 
ACAAAAGAAG AGTATATGAG GCCTTTTAAC GATTTTGTGA AAAGCAACTT TGATGTAGCA 
CGGAGATTTT TCCTTGATAT CGCATCTGAT TGTCCTGTAA GTGATGCCAT AAACCACAGC 
CTTTCCTTCA TCAGCGATGG CAATGTCTTG GCTTTGCACC GTCTGCTCTG GAACAACCAA 
GAGAAAATCG GCCAATATCT TTCAAGCAAC AGAGATCACA AAGCTGTTGG AAGGCGACCT 
TTCGATAAGA TGGCAACACT GCTTGCCTAC CTGGGACCTC CAGAGCACAA GCCTGTGGCT 
GACACACACT GGTCCAGCCT AAATCTTACC AGCTCCAAGT TTGAGGAATT CATGACAAGG 
CACCAGGTAC ATGAAAAAGA GGAATTCAAG GCTCTGAAAA CATTAAATAT CTTCTATCAA 
GCAGGCACTT CCAAAGCTGG AAATCCTGTT TTTTATTATG TCACACGAAG GTTCAAGACT 
GGCCAGATCA ACGGGGATTT GCTTATATAC CACGTCTTAC TAACTTTAAA GCCATATTAT 
GCAAAGCCAT ATGAGATTGT AATGGACCTC ACTCATGCCG GGCCTAGCAA TCGCTTTAAG 
ACAGACTTCC TCTCCAAGTG GTTTGTTGTT TTCCCGGGCT TTGCTTACGA GAACGTCTCT 
TCGGTCTACA TCTACAACTG TAATTCTTGG GTTCGGGAGT ACACCAAATA CCATGAGAGA 
CTGCTCACGG GACTCAAAGG CAGCAAAAGA CTCTTTTTCA TAGACTCTCC CTCAAAACTG 
GCTGAGCATA TCGATTATGA TCAGCAAAAA CTGCCAGCCG CGACCTTGGC TCTGGATGAA 
GACCTCAAGG TGTACAACAG TGCTCTCAAG CTGGCTCACA AGGACACCAA GGTGACCCTC 
AAGGTTGGCT CTACTGCGAT CCAAGTGACG TCATCTGAGC GAACTAAAGT CCTGGGACAG 
ACGGTTTTTC TGAATGATAT TTACTATGCC TCTGAGATCG AAGAAATCTG TCTTGTGGAT 
GAAAACCAGT TCACTCTGAC CATTTCCAAT CAGGGCACTC CCCTCACCTT CATGCATCAA 
GAGTGTGAAG GCATTGTGGC GTCCATCATT CACATCCGAA CTCGATGGGA GCTGTCCCAG 
CCAGATTCTA TCCCCCAGCA CACTAAGATC CGGCCAAAGG ACGTTCCCGG AACACTTTTG 
AATATCGCAT TACTTAACTT GGGTAGTTCG GACCCAAGTT TACGGTCAGC CGCCTATAAT 
CTTCTCTGTG CCTTAACCTG TACCTTTAAT TTAAAAATTG AGGGCCAATT ACTAGAAACA 
TCAGGTCTGT GTATACCTGC CAACAATACC CTTTTTATTG TCTCTATTAG TAAAACCCTG 
GCAGCTAATG AGCCACACCT CACCTTAGAG TTTTTGGAAG AGTGCATTTC CGGATTTAGC 
AAATCCAGTA TTGAACTGAA GCACCTGTGT CTAGAATACA TGACTCCCTG GCTATCAAAT 
CTAGTCCGTT TCTGTAAACA TAATGATGAT GCCAAACGAC AGAGAGTCAC TGCTATTCTT 
GATAAACTTA TAACAATGAC CATAAATGAA AAGCAGATGT ACCCATCGAT CCAAGCCAAG 
ATATGGGGAA GCCTTGGGCA GATTACAGAT CTGCTCGATG TAGTGCTGGA CAGCTTTATC 
AAAACCAGTG CCACAGGTGG TTTAGGATCC ATCAAGGCTG AAGTGATGGC TGACACGGCC 
GTGGCTCTGG CTTCTGGGAA CGTCAAACTA GTTTCGAGCA AGGTGATTGG AAGAATGTGC 
AAAATCATTG ACAAGACCTG CCTCTCTCCA ACTCCTACCT TAGAGCAGCA CCTCATGTGG 
GATGATATCG CCATTTTAGC ACGCTACATG CTAATGCTGT CCTTCAATAA TTCTCTGGAT 
GTGGCGGCTC ATCTTCCCTA CCTCTTCCAT GTGGTGACTC TGTTGGTGGC CACAGGTCCC 
TTGTCCCTTC GTGCTTCCAC ACACGGACTG GTCATCAATA TCATCCATTC TCTGTGCACG 
TGCTCACAGC TTCACTTTAG TGAAGAGACC AAGCAAGTTT TACGACTGAG TCTGACTGAG 
TTCTCACTAC CTAAGTTTTA TTTGCTGTTC GGCATCAGCA AAGTCAAATC AGCTGCCGTG 
ATAGCCTTCC GGTCCAGTTA CCGGGACAGG TCTTTCTCCC CTGGCTCCTA TGAGAGGGAG 
ACCTTTGCCC TGGCCTCCTT GGAAACAGTC ACAGAAGCTC TCTTGGAGAT CATGGAGGCT 
TGTATGAGAG ATATTCCTGC ATGCAAGTGG CTGGACCAGT GGACAGAGCT AGCCCAGAGG 
TTTGCGTTCC AGTACAATCC CTCCCTGCAG CCACGAGCTC TGGTTGTGTT TGGTTGTATC 
AGCAAAAGAG TGTCCCATGG GCAGATCAAG CAGATTATCC GCATTCTTAG CAAGGGACTT 
GAGAGCTGCC TAAAAGGACC TGATAATTAT AACAGTCAAG TTCTGATTGA AGCTACCGTG 
ATAGCTCTAA CCAAATTACA ACCACTTCTT AATAAGCAGG AGTCCCCTCT GCACCGAGCT 
CTCTTCTGGG TGGCCGTTGC CGTGCTGCAG CTGGATGAAG TGGACCTGTA CTCGGCGGGC 
ACTGCCCTCC TCGAACAGAA CCTGCACACC TTAGACAGCC TCCTGCACAT CTTCAACGAC 
CAGAGCCCAG AAGAAGTGTT CATGGAGATC CGGCAGCCTC TGGAGTGGCA CTGCAAGCAG 
ATGGACCACT TTGTGGGGCT CAACTTTAAC TCCAGCTTTA ACTTTGCCCT GGTCGGGCAC 
CTGCTGAAGG GATTTCGGCA TCCTTCACCT GTCACTGTTG CAAGAACTGT GAGGATTTTG 
CACACCCTAC TAGCATTGGT TAACAAGCAT AGGAATTGTG ACAAGTTTGA GGTGAACACC 
CAGAGTGTGG CTTACTTAGC AGCTTTACTT ACTGTGTCTG AGGAGGTTCG AAGTCGCTGC 
AGCTTAAAGC ATAGGAAATC ACTCCTACTA ACTGATATCA CAATGGAAAA TGTGCCTATG 
GATATATATC CCATCTATCA CAGTGACCCC AGCCTTAGGA CACTAAAAGA AACACAACCG 
TGGTCCTCTC CAAAGGGATC TGAGAGACAT CTTGCAGCCA GCTACCCTCC TGTGGGCCAA 
ACTAGCCCCC GAACCAGAAA ATCCATGAGC CTGGATATGG GGCAGCCTTC CCAGGCCAAC 
ACAAAGAAGT TGCTTGGGAC AAGGAAAAGC TTCGATCATT TGATCTCTGA TGCCAAAGCT 
CCCAAAAGGC AAGAACAGGA GTCTGGAGTC ACCACGCCCC CCAAGATGCG GAGAGTGGCC 
GAGAACGACT ACGAGATGGA AACCCAGAGA ATCTCCCCTC AGCCACACCC GCCTCTGCGG 
AAAGTGTCCG TGTCCGAATC CAATGTGCTG CTGGACGAGG AAGTGCTCAC AGACCCCAAA 
ACCCAGGCTC TTCTTCTCAC TGTCCTGGCT ACTCTGGTGA AGTACACTGC GGACGAGTTT 
GACCAGCGCA TTCTCTATGA GTACTTAGCG GAGGCCAGTG TTGTCTTCCC AAAGGTTTTT 
CCAGTGGTAC ATAATTTGTT GGACTCAAAG ATAAATACTC TGCTCTCATT ATGCCAAGAC 
CCAAATTTGC TAAACCCGGT TCATGGAATT GTGCAGAGTG TGGTCTACCA CGAAGAGGCC 
CCGCCGCAGT ACCAGACGGC GTACCTCCAG AGTTTCGGTT TTAATGGTCT GTGGAGGTTT 
GCAGGACCAT TTTCTAAGCA GACGCAGATC CCAGACTATG CCGAGCTCAT CGTGAAGTTT 
CTCGATGCCT TGATTGACAC GTATCTGCCT GGAATTGATG AGGAAGCCAG TGAGGAGTCC 
CTCCTGACAC CCACCTCTCC CTACCCGCCC GCGGTGCAGA GCCAGCTTAG CATCACTGCG 
AACCTCAACC TCTCTAACTC CATGACCTCA CTGGCAACTT CCCAGCATTC CCCAGGCATC 
GACAAGGAGA ATGTGGAGCT CTCGCCAACC ACCGGGCACT GCAGCGGCGG GAGGACGCGC 
CACGGCTCCG CCAGCCAGGT CCAGAAGCAA AGGAGTGCCG GCAGCTTCAA GAGGCACAGC 
ATCAAGAAGA TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823501.1
CDS	9..8666
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGVSDLPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKFSTLLTTITAWILSPWLCDTDVSIPLSCQITRPQSSFKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIVLGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKQESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967641.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967641.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCCAA AGGACACGAT GAGGTTAGAT 
GAAACAATGC TGGTCAAACA GTTGCTGCCG GAAATCTGCC ATTTCCTTCA CACCTACCGG 
GAAGGGAACC AGCACGCAGC CGAGCTTCGG AGCTCCGCCT CTGGCGTCCT TTTCTCCCTC 
AGCTGTAATA ACTTCAATGC CGTCTTCAGC CGGATTTCCA CCAGGTTACA GGAATTGACT 
GTTTGCTCAG AAGATAATGT TGATGTCCAT GATATAGAAT TATTACAATA TATCAATGTG 
GATTGTGCAA AACTAAAGCG GCTCTTGCAG GAAACTGTAT TTAAGTTTAA AGCCTTAAAG 
AAGGTAGCTC AACTAGCAGT TATAAATAGC CTAGAAAAGG CATTTTGGAA TTGGGTAGAG 
AACTATCCTG ATGAATTTAC AAAGCTGTAC CAGACTCCCC AGACAGATAT GGCGGAATGT 
GCAGAAAAAT TATTTGACTT GGTGGATGGT TTTGCTGAAA GCACCAAACG TAAAGCTGCA 
GTTTGGCCAC TGCAAATCAT TCTTCTTGTC TTGTGTCCAG AAATAATCCA GGACATTTCC 
AAGGATGTGG TTGATGAAGC GAAAATGAAT AAGTTTTCTA CACTGTTGAC CACTATCACC 
GCCTGGATAC TTTCTCCTTG GCTTTGTGAT ACCGATGTCT CAATTCCTCT TTCCTGCCAA 
ATCACTCGTC CTCAGTCCTC CTTTAAAATG TTTCTCGACA ATCTCAGAAG AGCGCTGGCC 
GGCCACAGTG GGAGCAGGCA GCTGACAGAG AGTGCCGCGA TCGCTTGTGT TAAACTGTGC 
AAAGCCAGCA CCTACATCAA CTGGGAGGAT AACTCGGTCA TTTTCCTGCT CGTCCAATCC 
ATGGTGGTGG ATCTGAAGAA CTTGCTCTTT AATCCCAGTA AGCCCTTCTC CAGAGGCAGC 
CAGAATGCTG ATGTGGACCT CATGATAGAC TGCCTGGTTT CTTGCTTTCG CATCAGCCCT 
CACAACAACC AACACTTTAA GATCTGTTTG GCCCAGAACT CCCCATCTAC ATTTCATTAT 
GTGCTGGTAA ACTCCCTCCA TCGAATTATC ACCAATAGCA CTTTCAAGCA TGGACTTGGC 
ACTGCTTCCG CCCTGGATTG GTGGCCCAAG ATTGATGCTG TGTATTGCCA TTCTGTAGAA 
CTTCGAAACA TGTTTGGTGA AACGCTTCAC AAGGCCGTGC AGGGCTGTGG GCCTCACCCC 
GCGATACGAA TGGCGCCGAG TCTTACATTT AAAGAAAAAA TGACAAGCCT TAAATTTAAA 
GAAAAACCTA CGGACTTGGA AACAAGAAGT TATAAATACC TCTTATTATC CATGGTGAAA 
CTAATCCATG CAGATCCAAA GCTGTTGCTA TGTAATCCAA GAAAACAAGG GCCTGAAACT 
CAGGGGAGTA CAGCAGAGTT AATCACAGGC CTTGTGCAGC TCGTCCCACA GTCCAGCATG 
CCAGAAATAG CTCAAGAAGC AATGGAGGCT TTGCTGGTTC TTCATCAGTT GGATAGTATT 
GACTTATGGA ATCCAGATTC ACCTATAGAA ACATTCTGGG AGATTAGCTC ACAAATGCTT 
TTTTATATCT GTAAAAAGCT AACTACCCAT CAGATGCTTA GCAGTACAGA AATTCTCAAG 
TGGTTGCGAG AAATTTTGAT CTGTAGGAAC AAATATCTTC TTAAAAATAA GCAGTCAGAT 
AGGAGTTCTT CCCATTTCCT CTACCTTTAT GGGGCAGGCT ATGATGTCTC TTCTGGTGGA 
GGTACCAGTC ATATCTCTCT AGACCACGAG GAGTTTATGC GTACCTGTGC TCATGGACCA 
TCTCTCAGGA AAGTGAAAGG AAGCTCCTCA ATGGACGGTG CAATCGGGTC CAGCGGGACG 
CCCATCTGCC GCCAGGCCCA GACTAAGCTG GAGGTTGCTC TGTACATGTT TCTGTGGAGT 
CCCGACATTG AAGCCGTGCT CGTGGCCATG TCCTGCTTTC GACACCTGTG TGAGGAAGCC 
GACATCAGGT GTGGCATAGA TGAGATCTCG GTGCACCATT TCTTGCCAAA CTATAACACC 
TTCATGGAGT TCGCATCTGT CAGCAACATG ATGTCGACAG GAAGAGCAGC TCTTCAGAAA 
AGAGTGATGG CCCTGCTCAG GCGCATCGAA CACCCCACCG CTGGAAACAC CGAGGCTTGG 
GAAGATACTT ACGCCAAATG GGAGCAAGCT ACAAAATCAA TCCTCAATTA TCCGAAAGCC 
AAAATTGAAG ATGGCCAGGT TGCTGAGAGT CTTCACAAGA CCATTGTGAA GAGAAGAATG 
TCTCACGTGA GTGGAGGGGG CTCCATAGAC TTGTGCGACA CTGACTCGCT ACAGGAGTGG 
ATCAACATGA CTGGGTTTCT CTGTGCCCTT GGTGGCGTGT GCCTACAGCA GCGGAGCAAT 
GCCGGCTTTG CCACCTACAG CCCTCCCATG GGCCCTATCA GTGAACGCAA GGGCTCTCTC 
ATTTCCATGG TGTCCAGCGA TGGAAACGCA GAGACGCCTG TCAGCAAGTT CATGGATCGG 
CTGCTCTCCT TGATGGTGTG TAACCACGAA AAAGTGGGGC TGCAGATACG AAGCAATGTC 
AAGGACTTGT TGGGTCTTGA ATTGAGTCCT GCCCTTTATC CAATGCTGTT TAACAAATTA 
AAAAATACCA TCAGCAAGTT TTTTGATCCT CAAGGACAGG TTTTATTGAC TGACACCAAC 
ACCCAGTTTG TGGAGCAGAC CATAGCCATA ATGAAGAACT TGCTTGATAA TCATGCTGAG 
GGTAGTTCTG AACACATGGG ACAAGCTAGC ATCGAAACTA TGATGTTAAA TCTGGTCAGA 
TATGTTAGAG TGTTTGGGAA TTTGGTGCAG GCAATTCAGA TCAAAACTAA GCTGTGCCAG 
CTGGTTCAAG TAATGATGGA GAGGAGAGAT GACCTTTCAT TTTGCCAAGA GATGAAATTT 
AGGAATAAAA TGGTAGAATA TCTGACAGAT TGGGTCATGG GAACATCTAA CCAAGCTGCA 
GATGAAGATG TGAAATGTCT AACTAGAGAT TTGGACCAGG CAAGCATGGA AGCAGTGGTG 
TCATTGCTCG CTGGGCTCCC TCTGCAGCCT GAGGAAGGCG ATGGGGTGGA ATTGATGGAA 
GCCAAATCCC AGCTGTTCCT TAAGTATTTC ACCCTCTTTA TGAACCTTCT TAATGACTGC 
AGTGAGGTCG AAGAAGAAAA TGTCCAGATT GGGGGCAGGA AGCGCGGCAT GTCGCGAAGG 
CTGGCGTCCC TGCGCCACTG CACCGTGCTG GCAATGTCCA ACCTGCTCAA TGCGAATGTA 
GACAGCGGCC TGATGCACTC TATAGTGCTA GGATTGGGTT ACCACAAAGA TCTTCAGACA 
AGAGCCACCT TCATGGAAGT CCTCACAAAA ATCCTTCAGC AAGGCACAGA GTTTGATACG 
CTCGCAGAAA CCGTGTTAGC TGATCGATTT GAGAGACTTG TTGAGTTGGT CACGATGATG 
GGTGATCAAG GAGAGCTCCC CATAGCCATG GCTCTGGCCA ACGTGGTGCC TTGTTCTCAG 
TGGGATGAAC TTGCTCGAGT CCTTGTTACT CTCTTTGATT CCCGACACTT ACTGTATCAG 
CTCCTTTGGA ACATGTTTTC TAAGGAAGTT GAGCTGGCAG ACTCCATGCA GACACTTTTT 
AGAGGAAACA GCTTGGCCAG TAAAATCATG ACCTTCTGCT TTAAGGTCTA CGGTGCTACA 
TACCTGCAGA AGCTTCTGGA GCCTTTACTC CGCTTTGTGA TCACATCCTC CGAATGGCAG 
CACGTCAGCT TCGAGGTCGA TCCCACCAGG TTAGAGCATA CTGAGAGCCT CGAGGACAAC 
CAGCGGAACC TTTTGCAAAT GACTGAGAAG TTCTTCCACG CCATCATCAG TTCTTCCTCC 
GAGTTCCCCC CTCAGCTACG CAGTGTGTGC CACTGCCTGT ACCAGGCAAC TTGCCACTCT 
CTACTGAATA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTTAGCCAG 
AGATTCCCTC AGAACAGCAT TGGCGCCGTG GGGAGTGCCA TGTTCCTGAG ATTCATCAAC 
CCTGCCGTTG TCTCTCCCTA CGAGGCAGGG ATTTTAGAGA GAAAACCGCT CCCTAGAATT 
GAAAGGGGGT TGAAGCTGAT GTCAAAGATT CTTCAGAGCA TTGCCAATCA TGTCCTTTTC 
ACAAAAGAAG AGTATATGAG GCCTTTTAAC GATTTTGTGA AAAGCAACTT TGATGTAGCA 
CGGAGATTTT TCCTTGATAT CGCATCTGAT TGTCCTGTAA GTGATGCCAT AAACCACAGC 
CTTTCCTTCA TCAGCGATGG CAATGTCTTG GCTTTGCACC GTCTGCTCTG GAACAACCAA 
GAGAAAATCG GCCAATATCT TTCAAGCAAC AGAGATCACA AAGCTGTTGG AAGGCGACCT 
TTCGATAAGA TGGCAACACT GCTTGCCTAC CTGGGACCTC CAGAGCACAA GCCTGTGGCT 
GACACACACT GGTCCAGCCT AAATCTTACC AGCTCCAAGT TTGAGGAATT CATGACAAGG 
CACCAGGTAC ATGAAAAAGA GGAATTCAAG GCTCTGAAAA CATTAAATAT CTTCTATCAA 
GCAGGCACTT CCAAAGCTGG AAATCCTGTT TTTTATTATG TCACACGAAG GTTCAAGACT 
GGCCAGATCA ACGGGGATTT GCTTATATAC CACGTCTTAC TAACTTTAAA GCCATATTAT 
GCAAAGCCAT ATGAGATTGT AATGGACCTC ACTCATGCCG GGCCTAGCAA TCGCTTTAAG 
ACAGACTTCC TCTCCAAGTG GTTTGTTGTT TTCCCGGGCT TTGCTTACGA GAACGTCTCT 
TCGGTCTACA TCTACAACTG TAATTCTTGG GTTCGGGAGT ACACCAAATA CCATGAGAGA 
CTGCTCACGG GACTCAAAGG CAGCAAAAGA CTCTTTTTCA TAGACTCTCC CTCAAAACTG 
GCTGAGCATA TCGATTATGA TCAGCAAAAA CTGCCAGCCG CGACCTTGGC TCTGGATGAA 
GACCTCAAGG TGTACAACAG TGCTCTCAAG CTGGCTCACA AGGACACCAA GGTGACCCTC 
AAGGTTGGCT CTACTGCGAT CCAAGTGACG TCATCTGAGC GAACTAAAGT CCTGGGACAG 
ACGGTTTTTC TGAATGATAT TTACTATGCC TCTGAGATCG AAGAAATCTG TCTTGTGGAT 
GAAAACCAGT TCACTCTGAC CATTTCCAAT CAGGGCACTC CCCTCACCTT CATGCATCAA 
GAGTGTGAAG GCATTGTGGC GTCCATCATT CACATCCGAA CTCGATGGGA GCTGTCCCAG 
CCAGATTCTA TCCCCCAGCA CACTAAGATC CGGCCAAAGG ACGTTCCCGG AACACTTTTG 
AATATCGCAT TACTTAACTT GGGTAGTTCG GACCCAAGTT TACGGTCAGC CGCCTATAAT 
CTTCTCTGTG CCTTAACCTG TACCTTTAAT TTAAAAATTG AGGGCCAATT ACTAGAAACA 
TCAGGTCTGT GTATACCTGC CAACAATACC CTTTTTATTG TCTCTATTAG TAAAACCCTG 
GCAGCTAATG AGCCACACCT CACCTTAGAG TTTTTGGAAG AGTGCATTTC CGGATTTAGC 
AAATCCAGTA TTGAACTGAA GCACCTGTGT CTAGAATACA TGACTCCCTG GCTATCAAAT 
CTAGTCCGTT TCTGTAAACA TAATGATGAT GCCAAACGAC AGAGAGTCAC TGCTATTCTT 
GATAAACTTA TAACAATGAC CATAAATGAA AAGCAGATGT ACCCATCGAT CCAAGCCAAG 
ATATGGGGAA GCCTTGGGCA GATTACAGAT CTGCTCGATG TAGTGCTGGA CAGCTTTATC 
AAAACCAGTG CCACAGGTGG TTTAGGATCC ATCAAGGCTG AAGTGATGGC TGACACGGCC 
GTGGCTCTGG CTTCTGGGAA CGTCAAACTA GTTTCGAGCA AGGTGATTGG AAGAATGTGC 
AAAATCATTG ACAAGACCTG CCTCTCTCCA ACTCCTACCT TAGAGCAGCA CCTCATGTGG 
GATGATATCG CCATTTTAGC ACGCTACATG CTAATGCTGT CCTTCAATAA TTCTCTGGAT 
GTGGCGGCTC ATCTTCCCTA CCTCTTCCAT GTGGTGACTC TGTTGGTGGC CACAGGTCCC 
TTGTCCCTTC GTGCTTCCAC ACACGGACTG GTCATCAATA TCATCCATTC TCTGTGCACG 
TGCTCACAGC TTCACTTTAG TGAAGAGACC AAGCAAGTTT TACGACTGAG TCTGACTGAG 
TTCTCACTAC CTAAGTTTTA TTTGCTGTTC GGCATCAGCA AAGTCAAATC AGCTGCCGTG 
ATAGCCTTCC GGTCCAGTTA CCGGGACAGG TCTTTCTCCC CTGGCTCCTA TGAGAGGGAG 
ACCTTTGCCC TGGCCTCCTT GGAAACAGTC ACAGAAGCTC TCTTGGAGAT CATGGAGGCT 
TGTATGAGAG ATATTCCTGC ATGCAAGTGG CTGGACCAGT GGACAGAGCT AGCCCAGAGG 
TTTGCGTTCC AGTACAATCC CTCCCTGCAG CCACGAGCTC TGGTTGTGTT TGGTTGTATC 
AGCAAAAGAG TGTCCCATGG GCAGATCAAG CAGATTATCC GCATTCTTAG CAAGGGACTT 
GAGAGCTGCC TAAAAGGACC TGATAATTAT AACAGTCAAG TTCTGATTGA AGCTACCGTG 
ATAGCTCTAA CCAAATTACA ACCACTTCTT AATAAGCAGG AGTCCCCTCT GCACCGAGCT 
CTCTTCTGGG TGGCCGTTGC CGTGCTGCAG CTGGATGAAG TGGACCTGTA CTCGGCGGGC 
ACTGCCCTCC TCGAACAGAA CCTGCACACC TTAGACAGCC TCCTGCACAT CTTCAACGAC 
CAGAGCCCAG AAGAAGTGTT CATGGAGATC CGGCAGCCTC TGGAGTGGCA CTGCAAGCAG 
ATGGACCACT TTGTGGGGCT CAACTTTAAC TCCAGCTTTA ACTTTGCCCT GGTCGGGCAC 
CTGCTGAAGG GATTTCGGCA TCCTTCACCT GTCACTGTTG CAAGAACTGT GAGGATTTTG 
CACACCCTAC TAGCATTGGT TAACAAGCAT AGGAATTGTG ACAAGTTTGA GGTGAACACC 
CAGAGTGTGG CTTACTTAGC AGCTTTACTT ACTGTGTCTG AGGAGGTTCG AAGTCGCTGC 
AGCTTAAAGC ATAGGAAATC ACTCCTACTA ACTGATATCA CAATGGAAAA TGTGCCTATG 
GATATATATC CCATCTATCA CAGTGACCCC AGCCTTAGGA CACTAAAAGA AACACAACCG 
TGGTCCTCTC CAAAGGGATC TGAGAGACAT CTTGCAGCCA GCTACCCTCC TGTGGGCCAA 
ACTAGCCCCC GAACCAGAAA ATCCATGAGC CTGGATATGG GGCAGCCTTC CCAGGCCAAC 
ACAAAGAAGT TGCTTGGGAC AAGGAAAAGC TTCGATCATT TGATCTCTGA TGCCAAAGCT 
CCCAAAAGGC AAGAACAGGA GTCTGGAGTC ACCACGCCCC CCAAGATGCG GAGAGTGGCC 
GAGAACGACT ACGAGATGGA AACCCAGAGA ATCTCCCCTC AGCCACACCC GCCTCTGCGG 
AAAGTGTCCG TGTCCGAATC CAATGTGCTG CTGGACGAGG AAGTGCTCAC AGACCCCAAA 
ACCCAGGCTC TTCTTCTCAC TGTCCTGGCT ACTCTGGTGA AGTACACTGC GGACGAGTTT 
GACCAGCGCA TTCTCTATGA GTACTTAGCG GAGGCCAGTG TTGTCTTCCC AAAGGTTTTT 
CCAGTGGTAC ATAATTTGTT GGACTCAAAG ATAAATACTC TGCTCTCATT ATGCCAAGAC 
CCAAATTTGC TAAACCCGGT TCATGGAATT GTGCAGAGTG TGGTCTACCA CGAAGAGGCC 
CCGCCGCAGT ACCAGACGGC GTACCTCCAG AGTTTCGGTT TTAATGGTCT GTGGAGGTTT 
GCAGGACCAT TTTCTAAGCA GACGCAGATC CCAGACTATG CCGAGCTCAT CGTGAAGTTT 
CTCGATGCCT TGATTGACAC GTATCTGCCT GGAATTGATG AGGAAGCCAG TGAGGAGTCC 
CTCCTGACAC CCACCTCTCC CTACCCGCCC GCGGTGCAGA GCCAGCTTAG CATCACTGCG 
AACCTCAACC TCTCTAACTC CATGACCTCA CTGGCAACTT CCCAGCATTC CCCAGGCATC 
GACAAGGAGA ATGTGGAGCT CTCGCCAACC ACCGGGCACT GCAGCGGCGG GAGGACGCGC 
CACGGCTCCG CCAGCCAGGT CCAGAAGCAA AGGAGTGCCG GCAGCTTCAA GAGGCACAGC 
ATCAAGAAGA TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967642.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823502.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X4, mRNA.

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CloneID	OSa234691
Clone ID Related Accession (Same CDS sequence)	XM_031967642.1
Accession Version	XM_031967642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGTCAGAT 
AGGAGTTCTT CCCATTTCCT CTACCTTTAT GGGGCAGGCT ATGATGTCTC TTCTGGTGGA 
GGTACCAGTC ATATCTCTCT AGACCACGAG GAGTTTATGC GTACCTGTGC TCATGGACCA 
TCTCTCAGGA AAGTGAAAGG AAGCTCCTCA ATGGACGGTG CAATCGGGTC CAGCGGGACG 
CCCATCTGCC GCCAGGCCCA GACTAAGCTG GAGGTTGCTC TGTACATGTT TCTGTGGAGT 
CCCGACATTG AAGCCGTGCT CGTGGCCATG TCCTGCTTTC GACACCTGTG TGAGGAAGCC 
GACATCAGGT GTGGCATAGA TGAGATCTCG GTGCACCATT TCTTGCCAAA CTATAACACC 
TTCATGGAGT TCGCATCTGT CAGCAACATG ATGTCGACAG GAAGAGCAGC TCTTCAGAAA 
AGAGTGATGG CCCTGCTCAG GCGCATCGAA CACCCCACCG CTGGAAACAC CGAGGCTTGG 
GAAGATACTT ACGCCAAATG GGAGCAAGCT ACAAAATCAA TCCTCAATTA TCCGAAAGCC 
AAAATTGAAG ATGGCCAGGT TGCTGAGAGT CTTCACAAGA CCATTGTGAA GAGAAGAATG 
TCTCACGTGA GTGGAGGGGG CTCCATAGAC TTGTGCGACA CTGACTCGCT ACAGGAGTGG 
ATCAACATGA CTGGGTTTCT CTGTGCCCTT GGTGGCGTGT GCCTACAGCA GCGGAGCAAT 
GCCGGCTTTG CCACCTACAG CCCTCCCATG GGCCCTATCA GTGAACGCAA GGGCTCTCTC 
ATTTCCATGG TGTCCAGCGA TGGAAACGCA GAGACGCCTG TCAGCAAGTT CATGGATCGG 
CTGCTCTCCT TGATGGTGTG TAACCACGAA AAAGTGGGGC TGCAGATACG AAGCAATGTC 
AAGGACTTGT TGGGTCTTGA ATTGAGTCCT GCCCTTTATC CAATGCTGTT TAACAAATTA 
AAAAATACCA TCAGCAAGTT TTTTGATCCT CAAGGACAGG TTTTATTGAC TGACACCAAC 
ACCCAGTTTG TGGAGCAGAC CATAGCCATA ATGAAGAACT TGCTTGATAA TCATGCTGAG 
GGTAGTTCTG AACACATGGG ACAAGCTAGC ATCGAAACTA TGATGTTAAA TCTGGTCAGA 
TATGTTAGAG TGTTTGGGAA TTTGGTGCAG GCAATTCAGA TCAAAACTAA GCTGTGCCAG 
CTGGTTCAAG TAATGATGGA GAGGAGAGAT GACCTTTCAT TTTGCCAAGA GATGAAATTT 
AGGAATAAAA TGGTAGAATA TCTGACAGAT TGGGTCATGG GAACATCTAA CCAAGCTGCA 
GATGAAGATG TGAAATGTCT AACTAGAGAT TTGGACCAGG CAAGCATGGA AGCAGTGGTG 
TCATTGCTCG CTGGGCTCCC TCTGCAGCCT GAGGAAGGCG ATGGGGTGGA ATTGATGGAA 
GCCAAATCCC AGCTGTTCCT TAAGTATTTC ACCCTCTTTA TGAACCTTCT TAATGACTGC 
AGTGAGGTCG AAGAAGAAAA TGTCCAGATT GGGGGCAGGA AGCGCGGCAT GTCGCGAAGG 
CTGGCGTCCC TGCGCCACTG CACCGTGCTG GCAATGTCCA ACCTGCTCAA TGCGAATGTA 
GACAGCGGCC TGATGCACTC TATAGTGCTA GGATTGGGTT ACCACAAAGA TCTTCAGACA 
AGAGCCACCT TCATGGAAGT CCTCACAAAA ATCCTTCAGC AAGGCACAGA GTTTGATACG 
CTCGCAGAAA CCGTGTTAGC TGATCGATTT GAGAGACTTG TTGAGTTGGT CACGATGATG 
GGTGATCAAG GAGAGCTCCC CATAGCCATG GCTCTGGCCA ACGTGGTGCC TTGTTCTCAG 
TGGGATGAAC TTGCTCGAGT CCTTGTTACT CTCTTTGATT CCCGACACTT ACTGTATCAG 
CTCCTTTGGA ACATGTTTTC TAAGGAAGTT GAGCTGGCAG ACTCCATGCA GACACTTTTT 
AGAGGAAACA GCTTGGCCAG TAAAATCATG ACCTTCTGCT TTAAGGTCTA CGGTGCTACA 
TACCTGCAGA AGCTTCTGGA GCCTTTACTC CGCTTTGTGA TCACATCCTC CGAATGGCAG 
CACGTCAGCT TCGAGGTCGA TCCCACCAGG TTAGAGCATA CTGAGAGCCT CGAGGACAAC 
CAGCGGAACC TTTTGCAAAT GACTGAGAAG TTCTTCCACG CCATCATCAG TTCTTCCTCC 
GAGTTCCCCC CTCAGCTACG CAGTGTGTGC CACTGCCTGT ACCAGGCAAC TTGCCACTCT 
CTACTGAATA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTTAGCCAG 
AGATTCCCTC AGAACAGCAT TGGCGCCGTG GGGAGTGCCA TGTTCCTGAG ATTCATCAAC 
CCTGCCGTTG TCTCTCCCTA CGAGGCAGGG ATTTTAGAGA GAAAACCGCT CCCTAGAATT 
GAAAGGGGGT TGAAGCTGAT GTCAAAGATT CTTCAGAGCA TTGCCAATCA TGTCCTTTTC 
ACAAAAGAAG AGTATATGAG GCCTTTTAAC GATTTTGTGA AAAGCAACTT TGATGTAGCA 
CGGAGATTTT TCCTTGATAT CGCATCTGAT TGTCCTGTAA GTGATGCCAT AAACCACAGC 
CTTTCCTTCA TCAGCGATGG CAATGTCTTG GCTTTGCACC GTCTGCTCTG GAACAACCAA 
GAGAAAATCG GCCAATATCT TTCAAGCAAC AGAGATCACA AAGCTGTTGG AAGGCGACCT 
TTCGATAAGA TGGCAACACT GCTTGCCTAC CTGGGACCTC CAGAGCACAA GCCTGTGGCT 
GACACACACT GGTCCAGCCT AAATCTTACC AGCTCCAAGT TTGAGGAATT CATGACAAGG 
CACCAGGTAC ATGAAAAAGA GGAATTCAAG GCTCTGAAAA CATTAAATAT CTTCTATCAA 
GCAGGCACTT CCAAAGCTGG AAATCCTGTT TTTTATTATG TCACACGAAG GTTCAAGACT 
GGCCAGATCA ACGGGGATTT GCTTATATAC CACGTCTTAC TAACTTTAAA GCCATATTAT 
GCAAAGCCAT ATGAGATTGT AATGGACCTC ACTCATGCCG GGCCTAGCAA TCGCTTTAAG 
ACAGACTTCC TCTCCAAGTG GTTTGTTGTT TTCCCGGGCT TTGCTTACGA GAACGTCTCT 
TCGGTCTACA TCTACAACTG TAATTCTTGG GTTCGGGAGT ACACCAAATA CCATGAGAGA 
CTGCTCACGG GACTCAAAGG CAGCAAAAGA CTCTTTTTCA TAGACTCTCC CTCAAAACTG 
GCTGAGCATA TCGATTATGA TCAGCAAAAA CTGCCAGCCG CGACCTTGGC TCTGGATGAA 
GACCTCAAGG TGTACAACAG TGCTCTCAAG CTGGCTCACA AGGACACCAA GGTGACCCTC 
AAGGTTGGCT CTACTGCGAT CCAAGTGACG TCATCTGAGC GAACTAAAGT CCTGGGACAG 
ACGGTTTTTC TGAATGATAT TTACTATGCC TCTGAGATCG AAGAAATCTG TCTTGTGGAT 
GAAAACCAGT TCACTCTGAC CATTTCCAAT CAGGGCACTC CCCTCACCTT CATGCATCAA 
GAGTGTGAAG GCATTGTGGC GTCCATCATT CACATCCGAA CTCGATGGGA GCTGTCCCAG 
CCAGATTCTA TCCCCCAGCA CACTAAGATC CGGCCAAAGG ACGTTCCCGG AACACTTTTG 
AATATCGCAT TACTTAACTT GGGTAGTTCG GACCCAAGTT TACGGTCAGC CGCCTATAAT 
CTTCTCTGTG CCTTAACCTG TACCTTTAAT TTAAAAATTG AGGGCCAATT ACTAGAAACA 
TCAGGTCTGT GTATACCTGC CAACAATACC CTTTTTATTG TCTCTATTAG TAAAACCCTG 
GCAGCTAATG AGCCACACCT CACCTTAGAG TTTTTGGAAG AGTGCATTTC CGGATTTAGC 
AAATCCAGTA TTGAACTGAA GCACCTGTGT CTAGAATACA TGACTCCCTG GCTATCAAAT 
CTAGTCCGTT TCTGTAAACA TAATGATGAT GCCAAACGAC AGAGAGTCAC TGCTATTCTT 
GATAAACTTA TAACAATGAC CATAAATGAA AAGCAGATGT ACCCATCGAT CCAAGCCAAG 
ATATGGGGAA GCCTTGGGCA GATTACAGAT CTGCTCGATG TAGTGCTGGA CAGCTTTATC 
AAAACCAGTG CCACAGGTGG TTTAGGATCC ATCAAGGCTG AAGTGATGGC TGACACGGCC 
GTGGCTCTGG CTTCTGGGAA CGTCAAACTA GTTTCGAGCA AGGTGATTGG AAGAATGTGC 
AAAATCATTG ACAAGACCTG CCTCTCTCCA ACTCCTACCT TAGAGCAGCA CCTCATGTGG 
GATGATATCG CCATTTTAGC ACGCTACATG CTAATGCTGT CCTTCAATAA TTCTCTGGAT 
GTGGCGGCTC ATCTTCCCTA CCTCTTCCAT GTGGTGACTC TGTTGGTGGC CACAGGTCCC 
TTGTCCCTTC GTGCTTCCAC ACACGGACTG GTCATCAATA TCATCCATTC TCTGTGCACG 
TGCTCACAGC TTCACTTTAG TGAAGAGACC AAGCAAGTTT TACGACTGAG TCTGACTGAG 
TTCTCACTAC CTAAGTTTTA TTTGCTGTTC GGCATCAGCA AAGTCAAATC AGCTGCCGTG 
ATAGCCTTCC GGTCCAGTTA CCGGGACAGG TCTTTCTCCC CTGGCTCCTA TGAGAGGGAG 
ACCTTTGCCC TGGCCTCCTT GGAAACAGTC ACAGAAGCTC TCTTGGAGAT CATGGAGGCT 
TGTATGAGAG ATATTCCTGC ATGCAAGTGG CTGGACCAGT GGACAGAGCT AGCCCAGAGG 
TTTGCGTTCC AGTACAATCC CTCCCTGCAG CCACGAGCTC TGGTTGTGTT TGGTTGTATC 
AGCAAAAGAG TGTCCCATGG GCAGATCAAG CAGATTATCC GCATTCTTAG CAAGGGACTT 
GAGAGCTGCC TAAAAGGACC TGATAATTAT AACAGTCAAG TTCTGATTGA AGCTACCGTG 
ATAGCTCTAA CCAAATTACA ACCACTTCTT AATAAGCAGG AGTCCCCTCT GCACCGAGCT 
CTCTTCTGGG TGGCCGTTGC CGTGCTGCAG CTGGATGAAG TGGACCTGTA CTCGGCGGGC 
ACTGCCCTCC TCGAACAGAA CCTGCACACC TTAGACAGCC TCCTGCACAT CTTCAACGAC 
CAGAGCCCAG AAGAAGTGTT CATGGAGATC CGGCAGCCTC TGGAGTGGCA CTGCAAGCAG 
ATGGACCACT TTGTGGGGCT CAACTTTAAC TCCAGCTTTA ACTTTGCCCT GGTCGGGCAC 
CTGCTGAAGG GATTTCGGCA TCCTTCACCT GTCACTGTTG CAAGAACTGT GAGGATTTTG 
CACACCCTAC TAGCATTGGT TAACAAGCAT AGGAATTGTG ACAAGTTTGA GGTGAACACC 
CAGAGTGTGG CTTACTTAGC AGCTTTACTT ACTGTGTCTG AGGAGGTTCG AAGTCGCTGC 
AGCTTAAAGC ATAGGAAATC ACTCCTACTA ACTGATATCA CAATGGAAAA TGTGCCTATG 
GATATATATC CCATCTATCA CAGTGACCCC AGCCTTAGGA CACTAAAAGA AACACAACCG 
TGGTCCTCTC CAAAGGGATC TGAGAGACAT CTTGCAGCCA GCTACCCTCC TGTGGGCCAA 
ACTAGCCCCC GAACCAGAAA ATCCATGAGC CTGGATATGG GGCAGCCTTC CCAGGCCAAC 
ACAAAGAAGT TGCTTGGGAC AAGGAAAAGC TTCGATCATT TGATCTCTGA TGCCAAAGCT 
CCCAAAAGGC AAGAACAGGA GTCTGGAGTC ACCACGCCCC CCAAGATGCG GAGAGTGGCC 
GAGAACGACT ACGAGATGGA AACCCAGAGA ATCTCCCCTC AGCCACACCC GCCTCTGCGG 
AAAGTGTCCG TGTCCGAATC CAATGTGCTG CTGGACGAGG AAGTGCTCAC AGACCCCAAA 
ACCCAGGCTC TTCTTCTCAC TGTCCTGGCT ACTCTGGTGA AGTACACTGC GGACGAGTTT 
GACCAGCGCA TTCTCTATGA GTACTTAGCG GAGGCCAGTG TTGTCTTCCC AAAGGTTTTT 
CCAGTGGTAC ATAATTTGTT GGACTCAAAG ATAAATACTC TGCTCTCATT ATGCCAAGAC 
CCAAATTTGC TAAACCCGGT TCATGGAATT GTGCAGAGTG TGGTCTACCA CGAAGAGGCC 
CCGCCGCAGT ACCAGACGGC GTACCTCCAG AGTTTCGGTT TTAATGGTCT GTGGAGGTTT 
GCAGGACCAT TTTCTAAGCA GACGCAGATC CCAGACTATG CCGAGCTCAT CGTGAAGTTT 
CTCGATGCCT TGATTGACAC GTATCTGCCT GGAATTGATG AGGAAGCCAG TGAGGAGTCC 
CTCCTGACAC CCACCTCTCC CTACCCGCCC GCGGTGCAGA GCCAGCTTAG CATCACTGCG 
AACCTCAACC TCTCTAACTC CATGACCTCA CTGGCAACTT CCCAGCATTC CCCAGGCATC 
GACAAGGAGA ATGTGGAGCT CTCGCCAACC ACCGGGCACT GCAGCGGCGG GAGGACGCGC 
CACGGCTCCG CCAGCCAGGT CCAGAAGCAA AGGAGTGCCG GCAGCTTCAA GAGGCACAGC 
ATCAAGAAGA TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823502.1
CDS	9..8666
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGVSDLQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKFSTLLTTITAWILSPWLCDTDVSIPLSCQITRPQSSFKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIVLGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKQESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967642.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967642.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGTCAGAT 
AGGAGTTCTT CCCATTTCCT CTACCTTTAT GGGGCAGGCT ATGATGTCTC TTCTGGTGGA 
GGTACCAGTC ATATCTCTCT AGACCACGAG GAGTTTATGC GTACCTGTGC TCATGGACCA 
TCTCTCAGGA AAGTGAAAGG AAGCTCCTCA ATGGACGGTG CAATCGGGTC CAGCGGGACG 
CCCATCTGCC GCCAGGCCCA GACTAAGCTG GAGGTTGCTC TGTACATGTT TCTGTGGAGT 
CCCGACATTG AAGCCGTGCT CGTGGCCATG TCCTGCTTTC GACACCTGTG TGAGGAAGCC 
GACATCAGGT GTGGCATAGA TGAGATCTCG GTGCACCATT TCTTGCCAAA CTATAACACC 
TTCATGGAGT TCGCATCTGT CAGCAACATG ATGTCGACAG GAAGAGCAGC TCTTCAGAAA 
AGAGTGATGG CCCTGCTCAG GCGCATCGAA CACCCCACCG CTGGAAACAC CGAGGCTTGG 
GAAGATACTT ACGCCAAATG GGAGCAAGCT ACAAAATCAA TCCTCAATTA TCCGAAAGCC 
AAAATTGAAG ATGGCCAGGT TGCTGAGAGT CTTCACAAGA CCATTGTGAA GAGAAGAATG 
TCTCACGTGA GTGGAGGGGG CTCCATAGAC TTGTGCGACA CTGACTCGCT ACAGGAGTGG 
ATCAACATGA CTGGGTTTCT CTGTGCCCTT GGTGGCGTGT GCCTACAGCA GCGGAGCAAT 
GCCGGCTTTG CCACCTACAG CCCTCCCATG GGCCCTATCA GTGAACGCAA GGGCTCTCTC 
ATTTCCATGG TGTCCAGCGA TGGAAACGCA GAGACGCCTG TCAGCAAGTT CATGGATCGG 
CTGCTCTCCT TGATGGTGTG TAACCACGAA AAAGTGGGGC TGCAGATACG AAGCAATGTC 
AAGGACTTGT TGGGTCTTGA ATTGAGTCCT GCCCTTTATC CAATGCTGTT TAACAAATTA 
AAAAATACCA TCAGCAAGTT TTTTGATCCT CAAGGACAGG TTTTATTGAC TGACACCAAC 
ACCCAGTTTG TGGAGCAGAC CATAGCCATA ATGAAGAACT TGCTTGATAA TCATGCTGAG 
GGTAGTTCTG AACACATGGG ACAAGCTAGC ATCGAAACTA TGATGTTAAA TCTGGTCAGA 
TATGTTAGAG TGTTTGGGAA TTTGGTGCAG GCAATTCAGA TCAAAACTAA GCTGTGCCAG 
CTGGTTCAAG TAATGATGGA GAGGAGAGAT GACCTTTCAT TTTGCCAAGA GATGAAATTT 
AGGAATAAAA TGGTAGAATA TCTGACAGAT TGGGTCATGG GAACATCTAA CCAAGCTGCA 
GATGAAGATG TGAAATGTCT AACTAGAGAT TTGGACCAGG CAAGCATGGA AGCAGTGGTG 
TCATTGCTCG CTGGGCTCCC TCTGCAGCCT GAGGAAGGCG ATGGGGTGGA ATTGATGGAA 
GCCAAATCCC AGCTGTTCCT TAAGTATTTC ACCCTCTTTA TGAACCTTCT TAATGACTGC 
AGTGAGGTCG AAGAAGAAAA TGTCCAGATT GGGGGCAGGA AGCGCGGCAT GTCGCGAAGG 
CTGGCGTCCC TGCGCCACTG CACCGTGCTG GCAATGTCCA ACCTGCTCAA TGCGAATGTA 
GACAGCGGCC TGATGCACTC TATAGTGCTA GGATTGGGTT ACCACAAAGA TCTTCAGACA 
AGAGCCACCT TCATGGAAGT CCTCACAAAA ATCCTTCAGC AAGGCACAGA GTTTGATACG 
CTCGCAGAAA CCGTGTTAGC TGATCGATTT GAGAGACTTG TTGAGTTGGT CACGATGATG 
GGTGATCAAG GAGAGCTCCC CATAGCCATG GCTCTGGCCA ACGTGGTGCC TTGTTCTCAG 
TGGGATGAAC TTGCTCGAGT CCTTGTTACT CTCTTTGATT CCCGACACTT ACTGTATCAG 
CTCCTTTGGA ACATGTTTTC TAAGGAAGTT GAGCTGGCAG ACTCCATGCA GACACTTTTT 
AGAGGAAACA GCTTGGCCAG TAAAATCATG ACCTTCTGCT TTAAGGTCTA CGGTGCTACA 
TACCTGCAGA AGCTTCTGGA GCCTTTACTC CGCTTTGTGA TCACATCCTC CGAATGGCAG 
CACGTCAGCT TCGAGGTCGA TCCCACCAGG TTAGAGCATA CTGAGAGCCT CGAGGACAAC 
CAGCGGAACC TTTTGCAAAT GACTGAGAAG TTCTTCCACG CCATCATCAG TTCTTCCTCC 
GAGTTCCCCC CTCAGCTACG CAGTGTGTGC CACTGCCTGT ACCAGGCAAC TTGCCACTCT 
CTACTGAATA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTTAGCCAG 
AGATTCCCTC AGAACAGCAT TGGCGCCGTG GGGAGTGCCA TGTTCCTGAG ATTCATCAAC 
CCTGCCGTTG TCTCTCCCTA CGAGGCAGGG ATTTTAGAGA GAAAACCGCT CCCTAGAATT 
GAAAGGGGGT TGAAGCTGAT GTCAAAGATT CTTCAGAGCA TTGCCAATCA TGTCCTTTTC 
ACAAAAGAAG AGTATATGAG GCCTTTTAAC GATTTTGTGA AAAGCAACTT TGATGTAGCA 
CGGAGATTTT TCCTTGATAT CGCATCTGAT TGTCCTGTAA GTGATGCCAT AAACCACAGC 
CTTTCCTTCA TCAGCGATGG CAATGTCTTG GCTTTGCACC GTCTGCTCTG GAACAACCAA 
GAGAAAATCG GCCAATATCT TTCAAGCAAC AGAGATCACA AAGCTGTTGG AAGGCGACCT 
TTCGATAAGA TGGCAACACT GCTTGCCTAC CTGGGACCTC CAGAGCACAA GCCTGTGGCT 
GACACACACT GGTCCAGCCT AAATCTTACC AGCTCCAAGT TTGAGGAATT CATGACAAGG 
CACCAGGTAC ATGAAAAAGA GGAATTCAAG GCTCTGAAAA CATTAAATAT CTTCTATCAA 
GCAGGCACTT CCAAAGCTGG AAATCCTGTT TTTTATTATG TCACACGAAG GTTCAAGACT 
GGCCAGATCA ACGGGGATTT GCTTATATAC CACGTCTTAC TAACTTTAAA GCCATATTAT 
GCAAAGCCAT ATGAGATTGT AATGGACCTC ACTCATGCCG GGCCTAGCAA TCGCTTTAAG 
ACAGACTTCC TCTCCAAGTG GTTTGTTGTT TTCCCGGGCT TTGCTTACGA GAACGTCTCT 
TCGGTCTACA TCTACAACTG TAATTCTTGG GTTCGGGAGT ACACCAAATA CCATGAGAGA 
CTGCTCACGG GACTCAAAGG CAGCAAAAGA CTCTTTTTCA TAGACTCTCC CTCAAAACTG 
GCTGAGCATA TCGATTATGA TCAGCAAAAA CTGCCAGCCG CGACCTTGGC TCTGGATGAA 
GACCTCAAGG TGTACAACAG TGCTCTCAAG CTGGCTCACA AGGACACCAA GGTGACCCTC 
AAGGTTGGCT CTACTGCGAT CCAAGTGACG TCATCTGAGC GAACTAAAGT CCTGGGACAG 
ACGGTTTTTC TGAATGATAT TTACTATGCC TCTGAGATCG AAGAAATCTG TCTTGTGGAT 
GAAAACCAGT TCACTCTGAC CATTTCCAAT CAGGGCACTC CCCTCACCTT CATGCATCAA 
GAGTGTGAAG GCATTGTGGC GTCCATCATT CACATCCGAA CTCGATGGGA GCTGTCCCAG 
CCAGATTCTA TCCCCCAGCA CACTAAGATC CGGCCAAAGG ACGTTCCCGG AACACTTTTG 
AATATCGCAT TACTTAACTT GGGTAGTTCG GACCCAAGTT TACGGTCAGC CGCCTATAAT 
CTTCTCTGTG CCTTAACCTG TACCTTTAAT TTAAAAATTG AGGGCCAATT ACTAGAAACA 
TCAGGTCTGT GTATACCTGC CAACAATACC CTTTTTATTG TCTCTATTAG TAAAACCCTG 
GCAGCTAATG AGCCACACCT CACCTTAGAG TTTTTGGAAG AGTGCATTTC CGGATTTAGC 
AAATCCAGTA TTGAACTGAA GCACCTGTGT CTAGAATACA TGACTCCCTG GCTATCAAAT 
CTAGTCCGTT TCTGTAAACA TAATGATGAT GCCAAACGAC AGAGAGTCAC TGCTATTCTT 
GATAAACTTA TAACAATGAC CATAAATGAA AAGCAGATGT ACCCATCGAT CCAAGCCAAG 
ATATGGGGAA GCCTTGGGCA GATTACAGAT CTGCTCGATG TAGTGCTGGA CAGCTTTATC 
AAAACCAGTG CCACAGGTGG TTTAGGATCC ATCAAGGCTG AAGTGATGGC TGACACGGCC 
GTGGCTCTGG CTTCTGGGAA CGTCAAACTA GTTTCGAGCA AGGTGATTGG AAGAATGTGC 
AAAATCATTG ACAAGACCTG CCTCTCTCCA ACTCCTACCT TAGAGCAGCA CCTCATGTGG 
GATGATATCG CCATTTTAGC ACGCTACATG CTAATGCTGT CCTTCAATAA TTCTCTGGAT 
GTGGCGGCTC ATCTTCCCTA CCTCTTCCAT GTGGTGACTC TGTTGGTGGC CACAGGTCCC 
TTGTCCCTTC GTGCTTCCAC ACACGGACTG GTCATCAATA TCATCCATTC TCTGTGCACG 
TGCTCACAGC TTCACTTTAG TGAAGAGACC AAGCAAGTTT TACGACTGAG TCTGACTGAG 
TTCTCACTAC CTAAGTTTTA TTTGCTGTTC GGCATCAGCA AAGTCAAATC AGCTGCCGTG 
ATAGCCTTCC GGTCCAGTTA CCGGGACAGG TCTTTCTCCC CTGGCTCCTA TGAGAGGGAG 
ACCTTTGCCC TGGCCTCCTT GGAAACAGTC ACAGAAGCTC TCTTGGAGAT CATGGAGGCT 
TGTATGAGAG ATATTCCTGC ATGCAAGTGG CTGGACCAGT GGACAGAGCT AGCCCAGAGG 
TTTGCGTTCC AGTACAATCC CTCCCTGCAG CCACGAGCTC TGGTTGTGTT TGGTTGTATC 
AGCAAAAGAG TGTCCCATGG GCAGATCAAG CAGATTATCC GCATTCTTAG CAAGGGACTT 
GAGAGCTGCC TAAAAGGACC TGATAATTAT AACAGTCAAG TTCTGATTGA AGCTACCGTG 
ATAGCTCTAA CCAAATTACA ACCACTTCTT AATAAGCAGG AGTCCCCTCT GCACCGAGCT 
CTCTTCTGGG TGGCCGTTGC CGTGCTGCAG CTGGATGAAG TGGACCTGTA CTCGGCGGGC 
ACTGCCCTCC TCGAACAGAA CCTGCACACC TTAGACAGCC TCCTGCACAT CTTCAACGAC 
CAGAGCCCAG AAGAAGTGTT CATGGAGATC CGGCAGCCTC TGGAGTGGCA CTGCAAGCAG 
ATGGACCACT TTGTGGGGCT CAACTTTAAC TCCAGCTTTA ACTTTGCCCT GGTCGGGCAC 
CTGCTGAAGG GATTTCGGCA TCCTTCACCT GTCACTGTTG CAAGAACTGT GAGGATTTTG 
CACACCCTAC TAGCATTGGT TAACAAGCAT AGGAATTGTG ACAAGTTTGA GGTGAACACC 
CAGAGTGTGG CTTACTTAGC AGCTTTACTT ACTGTGTCTG AGGAGGTTCG AAGTCGCTGC 
AGCTTAAAGC ATAGGAAATC ACTCCTACTA ACTGATATCA CAATGGAAAA TGTGCCTATG 
GATATATATC CCATCTATCA CAGTGACCCC AGCCTTAGGA CACTAAAAGA AACACAACCG 
TGGTCCTCTC CAAAGGGATC TGAGAGACAT CTTGCAGCCA GCTACCCTCC TGTGGGCCAA 
ACTAGCCCCC GAACCAGAAA ATCCATGAGC CTGGATATGG GGCAGCCTTC CCAGGCCAAC 
ACAAAGAAGT TGCTTGGGAC AAGGAAAAGC TTCGATCATT TGATCTCTGA TGCCAAAGCT 
CCCAAAAGGC AAGAACAGGA GTCTGGAGTC ACCACGCCCC CCAAGATGCG GAGAGTGGCC 
GAGAACGACT ACGAGATGGA AACCCAGAGA ATCTCCCCTC AGCCACACCC GCCTCTGCGG 
AAAGTGTCCG TGTCCGAATC CAATGTGCTG CTGGACGAGG AAGTGCTCAC AGACCCCAAA 
ACCCAGGCTC TTCTTCTCAC TGTCCTGGCT ACTCTGGTGA AGTACACTGC GGACGAGTTT 
GACCAGCGCA TTCTCTATGA GTACTTAGCG GAGGCCAGTG TTGTCTTCCC AAAGGTTTTT 
CCAGTGGTAC ATAATTTGTT GGACTCAAAG ATAAATACTC TGCTCTCATT ATGCCAAGAC 
CCAAATTTGC TAAACCCGGT TCATGGAATT GTGCAGAGTG TGGTCTACCA CGAAGAGGCC 
CCGCCGCAGT ACCAGACGGC GTACCTCCAG AGTTTCGGTT TTAATGGTCT GTGGAGGTTT 
GCAGGACCAT TTTCTAAGCA GACGCAGATC CCAGACTATG CCGAGCTCAT CGTGAAGTTT 
CTCGATGCCT TGATTGACAC GTATCTGCCT GGAATTGATG AGGAAGCCAG TGAGGAGTCC 
CTCCTGACAC CCACCTCTCC CTACCCGCCC GCGGTGCAGA GCCAGCTTAG CATCACTGCG 
AACCTCAACC TCTCTAACTC CATGACCTCA CTGGCAACTT CCCAGCATTC CCCAGGCATC 
GACAAGGAGA ATGTGGAGCT CTCGCCAACC ACCGGGCACT GCAGCGGCGG GAGGACGCGC 
CACGGCTCCG CCAGCCAGGT CCAGAAGCAA AGGAGTGCCG GCAGCTTCAA GAGGCACAGC 
ATCAAGAAGA TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967643.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823503.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X5, mRNA.

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CloneID	OSa234692
Clone ID Related Accession (Same CDS sequence)	XM_031967643.1
Accession Version	XM_031967643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGCAGTCA 
GATAGGAGTT CTTCCCATTT CCTCTACCTT TATGGGGCAG GCTATGATGT CTCTTCTGGT 
GGAGGTACCA GTCATATCTC TCTAGACCAC GAGGAGTTTA TGCGTACCTG TGCTCATGGA 
CCATCTCTCA GGAAAGTGAA AGGAAGCTCC TCAATGGACG GTGCAATCGG GTCCAGCGGG 
ACGCCCATCT GCCGCCAGGC CCAGACTAAG CTGGAGGTTG CTCTGTACAT GTTTCTGTGG 
AGTCCCGACA TTGAAGCCGT GCTCGTGGCC ATGTCCTGCT TTCGACACCT GTGTGAGGAA 
GCCGACATCA GGTGTGGCAT AGATGAGATC TCGGTGCACC ATTTCTTGCC AAACTATAAC 
ACCTTCATGG AGTTCGCATC TGTCAGCAAC ATGATGTCGA CAGGAAGAGC AGCTCTTCAG 
AAAAGAGTGA TGGCCCTGCT CAGGCGCATC GAACACCCCA CCGCTGGAAA CACCGAGGCT 
TGGGAAGATA CTTACGCCAA ATGGGAGCAA GCTACAAAAT CAATCCTCAA TTATCCGAAA 
GCCAAAATTG AAGATGGCCA GGTTGCTGAG AGTCTTCACA AGACCATTGT GAAGAGAAGA 
ATGTCTCACG TGAGTGGAGG GGGCTCCATA GACTTGTGCG ACACTGACTC GCTACAGGAG 
TGGATCAACA TGACTGGGTT TCTCTGTGCC CTTGGTGGCG TGTGCCTACA GCAGCGGAGC 
AATGCCGGCT TTGCCACCTA CAGCCCTCCC ATGGGCCCTA TCAGTGAACG CAAGGGCTCT 
CTCATTTCCA TGGTGTCCAG CGATGGAAAC GCAGAGACGC CTGTCAGCAA GTTCATGGAT 
CGGCTGCTCT CCTTGATGGT GTGTAACCAC GAAAAAGTGG GGCTGCAGAT ACGAAGCAAT 
GTCAAGGACT TGTTGGGTCT TGAATTGAGT CCTGCCCTTT ATCCAATGCT GTTTAACAAA 
TTAAAAAATA CCATCAGCAA GTTTTTTGAT CCTCAAGGAC AGGTTTTATT GACTGACACC 
AACACCCAGT TTGTGGAGCA GACCATAGCC ATAATGAAGA ACTTGCTTGA TAATCATGCT 
GAGGGTAGTT CTGAACACAT GGGACAAGCT AGCATCGAAA CTATGATGTT AAATCTGGTC 
AGATATGTTA GAGTGTTTGG GAATTTGGTG CAGGCAATTC AGATCAAAAC TAAGCTGTGC 
CAGCTGGTTC AAGTAATGAT GGAGAGGAGA GATGACCTTT CATTTTGCCA AGAGATGAAA 
TTTAGGAATA AAATGGTAGA ATATCTGACA GATTGGGTCA TGGGAACATC TAACCAAGCT 
GCAGATGAAG ATGTGAAATG TCTAACTAGA GATTTGGACC AGGCAAGCAT GGAAGCAGTG 
GTGTCATTGC TCGCTGGGCT CCCTCTGCAG CCTGAGGAAG GCGATGGGGT GGAATTGATG 
GAAGCCAAAT CCCAGCTGTT CCTTAAGTAT TTCACCCTCT TTATGAACCT TCTTAATGAC 
TGCAGTGAGG TCGAAGAAGA AAATGTCCAG ATTGGGGGCA GGAAGCGCGG CATGTCGCGA 
AGGCTGGCGT CCCTGCGCCA CTGCACCGTG CTGGCAATGT CCAACCTGCT CAATGCGAAT 
GTAGACAGCG GCCTGATGCA CTCTATAGTG CTAGGATTGG GTTACCACAA AGATCTTCAG 
ACAAGAGCCA CCTTCATGGA AGTCCTCACA AAAATCCTTC AGCAAGGCAC AGAGTTTGAT 
ACGCTCGCAG AAACCGTGTT AGCTGATCGA TTTGAGAGAC TTGTTGAGTT GGTCACGATG 
ATGGGTGATC AAGGAGAGCT CCCCATAGCC ATGGCTCTGG CCAACGTGGT GCCTTGTTCT 
CAGTGGGATG AACTTGCTCG AGTCCTTGTT ACTCTCTTTG ATTCCCGACA CTTACTGTAT 
CAGCTCCTTT GGAACATGTT TTCTAAGGAA GTTGAGCTGG CAGACTCCAT GCAGACACTT 
TTTAGAGGAA ACAGCTTGGC CAGTAAAATC ATGACCTTCT GCTTTAAGGT CTACGGTGCT 
ACATACCTGC AGAAGCTTCT GGAGCCTTTA CTCCGCTTTG TGATCACATC CTCCGAATGG 
CAGCACGTCA GCTTCGAGGT CGATCCCACC AGGTTAGAGC ATACTGAGAG CCTCGAGGAC 
AACCAGCGGA ACCTTTTGCA AATGACTGAG AAGTTCTTCC ACGCCATCAT CAGTTCTTCC 
TCCGAGTTCC CCCCTCAGCT ACGCAGTGTG TGCCACTGCC TGTACCAGGC AACTTGCCAC 
TCTCTACTGA ATAAAGCTAC CGTAAAAGAA AAAAAGGAAA ACAAAAAATC AGTTGTTAGC 
CAGAGATTCC CTCAGAACAG CATTGGCGCC GTGGGGAGTG CCATGTTCCT GAGATTCATC 
AACCCTGCCG TTGTCTCTCC CTACGAGGCA GGGATTTTAG AGAGAAAACC GCTCCCTAGA 
ATTGAAAGGG GGTTGAAGCT GATGTCAAAG ATTCTTCAGA GCATTGCCAA TCATGTCCTT 
TTCACAAAAG AAGAGTATAT GAGGCCTTTT AACGATTTTG TGAAAAGCAA CTTTGATGTA 
GCACGGAGAT TTTTCCTTGA TATCGCATCT GATTGTCCTG TAAGTGATGC CATAAACCAC 
AGCCTTTCCT TCATCAGCGA TGGCAATGTC TTGGCTTTGC ACCGTCTGCT CTGGAACAAC 
CAAGAGAAAA TCGGCCAATA TCTTTCAAGC AACAGAGATC ACAAAGCTGT TGGAAGGCGA 
CCTTTCGATA AGATGGCAAC ACTGCTTGCC TACCTGGGAC CTCCAGAGCA CAAGCCTGTG 
GCTGACACAC ACTGGTCCAG CCTAAATCTT ACCAGCTCCA AGTTTGAGGA ATTCATGACA 
AGGCACCAGG TACATGAAAA AGAGGAATTC AAGGCTCTGA AAACATTAAA TATCTTCTAT 
CAAGCAGGCA CTTCCAAAGC TGGAAATCCT GTTTTTTATT ATGTCACACG AAGGTTCAAG 
ACTGGCCAGA TCAACGGGGA TTTGCTTATA TACCACGTCT TACTAACTTT AAAGCCATAT 
TATGCAAAGC CATATGAGAT TGTAATGGAC CTCACTCATG CCGGGCCTAG CAATCGCTTT 
AAGACAGACT TCCTCTCCAA GTGGTTTGTT GTTTTCCCGG GCTTTGCTTA CGAGAACGTC 
TCTTCGGTCT ACATCTACAA CTGTAATTCT TGGGTTCGGG AGTACACCAA ATACCATGAG 
AGACTGCTCA CGGGACTCAA AGGCAGCAAA AGACTCTTTT TCATAGACTC TCCCTCAAAA 
CTGGCTGAGC ATATCGATTA TGATCAGCAA AAACTGCCAG CCGCGACCTT GGCTCTGGAT 
GAAGACCTCA AGGTGTACAA CAGTGCTCTC AAGCTGGCTC ACAAGGACAC CAAGGTGACC 
CTCAAGGTTG GCTCTACTGC GATCCAAGTG ACGTCATCTG AGCGAACTAA AGTCCTGGGA 
CAGACGGTTT TTCTGAATGA TATTTACTAT GCCTCTGAGA TCGAAGAAAT CTGTCTTGTG 
GATGAAAACC AGTTCACTCT GACCATTTCC AATCAGGGCA CTCCCCTCAC CTTCATGCAT 
CAAGAGTGTG AAGGCATTGT GGCGTCCATC ATTCACATCC GAACTCGATG GGAGCTGTCC 
CAGCCAGATT CTATCCCCCA GCACACTAAG ATCCGGCCAA AGGACGTTCC CGGAACACTT 
TTGAATATCG CATTACTTAA CTTGGGTAGT TCGGACCCAA GTTTACGGTC AGCCGCCTAT 
AATCTTCTCT GTGCCTTAAC CTGTACCTTT AATTTAAAAA TTGAGGGCCA ATTACTAGAA 
ACATCAGGTC TGTGTATACC TGCCAACAAT ACCCTTTTTA TTGTCTCTAT TAGTAAAACC 
CTGGCAGCTA ATGAGCCACA CCTCACCTTA GAGTTTTTGG AAGAGTGCAT TTCCGGATTT 
AGCAAATCCA GTATTGAACT GAAGCACCTG TGTCTAGAAT ACATGACTCC CTGGCTATCA 
AATCTAGTCC GTTTCTGTAA ACATAATGAT GATGCCAAAC GACAGAGAGT CACTGCTATT 
CTTGATAAAC TTATAACAAT GACCATAAAT GAAAAGCAGA TGTACCCATC GATCCAAGCC 
AAGATATGGG GAAGCCTTGG GCAGATTACA GATCTGCTCG ATGTAGTGCT GGACAGCTTT 
ATCAAAACCA GTGCCACAGG TGGTTTAGGA TCCATCAAGG CTGAAGTGAT GGCTGACACG 
GCCGTGGCTC TGGCTTCTGG GAACGTCAAA CTAGTTTCGA GCAAGGTGAT TGGAAGAATG 
TGCAAAATCA TTGACAAGAC CTGCCTCTCT CCAACTCCTA CCTTAGAGCA GCACCTCATG 
TGGGATGATA TCGCCATTTT AGCACGCTAC ATGCTAATGC TGTCCTTCAA TAATTCTCTG 
GATGTGGCGG CTCATCTTCC CTACCTCTTC CATGTGGTGA CTCTGTTGGT GGCCACAGGT 
CCCTTGTCCC TTCGTGCTTC CACACACGGA CTGGTCATCA ATATCATCCA TTCTCTGTGC 
ACGTGCTCAC AGCTTCACTT TAGTGAAGAG ACCAAGCAAG TTTTACGACT GAGTCTGACT 
GAGTTCTCAC TACCTAAGTT TTATTTGCTG TTCGGCATCA GCAAAGTCAA ATCAGCTGCC 
GTGATAGCCT TCCGGTCCAG TTACCGGGAC AGGTCTTTCT CCCCTGGCTC CTATGAGAGG 
GAGACCTTTG CCCTGGCCTC CTTGGAAACA GTCACAGAAG CTCTCTTGGA GATCATGGAG 
GCTTGTATGA GAGATATTCC TGCATGCAAG TGGCTGGACC AGTGGACAGA GCTAGCCCAG 
AGGTTTGCGT TCCAGTACAA TCCCTCCCTG CAGCCACGAG CTCTGGTTGT GTTTGGTTGT 
ATCAGCAAAA GAGTGTCCCA TGGGCAGATC AAGCAGATTA TCCGCATTCT TAGCAAGGGA 
CTTGAGAGCT GCCTAAAAGG ACCTGATAAT TATAACAGTC AAGTTCTGAT TGAAGCTACC 
GTGATAGCTC TAACCAAATT ACAACCACTT CTTAATAAGC AGGAGTCCCC TCTGCACCGA 
GCTCTCTTCT GGGTGGCCGT TGCCGTGCTG CAGCTGGATG AAGTGGACCT GTACTCGGCG 
GGCACTGCCC TCCTCGAACA GAACCTGCAC ACCTTAGACA GCCTCCTGCA CATCTTCAAC 
GACCAGAGCC CAGAAGAAGT GTTCATGGAG ATCCGGCAGC CTCTGGAGTG GCACTGCAAG 
CAGATGGACC ACTTTGTGGG GCTCAACTTT AACTCCAGCT TTAACTTTGC CCTGGTCGGG 
CACCTGCTGA AGGGATTTCG GCATCCTTCA CCTGTCACTG TTGCAAGAAC TGTGAGGATT 
TTGCACACCC TACTAGCATT GGTTAACAAG CATAGGAATT GTGACAAGTT TGAGGTGAAC 
ACCCAGAGTG TGGCTTACTT AGCAGCTTTA CTTACTGTGT CTGAGGAGGT TCGAAGTCGC 
TGCAGCTTAA AGCATAGGAA ATCACTCCTA CTAACTGATA TCACAATGGA AAATGTGCCT 
ATGGATATAT ATCCCATCTA TCACAGTGAC CCCAGCCTTA GGACACTAAA AGAAACACAA 
CCGTGGTCCT CTCCAAAGGG ATCTGAGAGA CATCTTGCAG CCAGCTACCC TCCTGTGGGC 
CAAACTAGCC CCCGAACCAG AAAATCCATG AGCCTGGATA TGGGGCAGCC TTCCCAGGCC 
AACACAAAGA AGTTGCTTGG GACAAGGAAA AGCTTCGATC ATTTGATCTC TGATGCCAAA 
GCTCCCAAAA GGCAAGAACA GGAGTCTGGA GTCACCACGC CCCCCAAGAT GCGGAGAGTG 
GCCGAGAACG ACTACGAGAT GGAAACCCAG AGAATCTCCC CTCAGCCACA CCCGCCTCTG 
CGGAAAGTGT CCGTGTCCGA ATCCAATGTG CTGCTGGACG AGGAAGTGCT CACAGACCCC 
AAAACCCAGG CTCTTCTTCT CACTGTCCTG GCTACTCTGG TGAAGTACAC TGCGGACGAG 
TTTGACCAGC GCATTCTCTA TGAGTACTTA GCGGAGGCCA GTGTTGTCTT CCCAAAGGTT 
TTTCCAGTGG TACATAATTT GTTGGACTCA AAGATAAATA CTCTGCTCTC ATTATGCCAA 
GACCCAAATT TGCTAAACCC GGTTCATGGA ATTGTGCAGA GTGTGGTCTA CCACGAAGAG 
GCCCCGCCGC AGTACCAGAC GGCGTACCTC CAGAGTTTCG GTTTTAATGG TCTGTGGAGG 
TTTGCAGGAC CATTTTCTAA GACGCAGATC CCAGACTATG CCGAGCTCAT CGTGAAGTTT 
CTCGATGCCT TGATTGACAC GTATCTGCCT GGAATTGATG AGGAAGCCAG TGAGGAGTCC 
CTCCTGACAC CCACCTCTCC CTACCCGCCC GCGGTGCAGA GCCAGCTTAG CATCACTGCG 
AACCTCAACC TCTCTAACTC CATGACCTCA CTGGCAACTT CCCAGCATTC CCCAGGCATC 
GACAAGGAGA ATGTGGAGCT CTCGCCAACC ACCGGGCACT GCAGCGGCGG GAGGACGCGC 
CACGGCTCCG CCAGCCAGGT CCAGAAGCAA AGGAGTGCCG GCAGCTTCAA GAGGCACAGC 
ATCAAGAAGA TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823503.1
CDS	9..8666
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGVSDLQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKFSTLLTTITAWILSPWLCDTDVSIPLSCQITRPQSSFKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIVLGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKQESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967643.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967643.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGCAGTCA 
GATAGGAGTT CTTCCCATTT CCTCTACCTT TATGGGGCAG GCTATGATGT CTCTTCTGGT 
GGAGGTACCA GTCATATCTC TCTAGACCAC GAGGAGTTTA TGCGTACCTG TGCTCATGGA 
CCATCTCTCA GGAAAGTGAA AGGAAGCTCC TCAATGGACG GTGCAATCGG GTCCAGCGGG 
ACGCCCATCT GCCGCCAGGC CCAGACTAAG CTGGAGGTTG CTCTGTACAT GTTTCTGTGG 
AGTCCCGACA TTGAAGCCGT GCTCGTGGCC ATGTCCTGCT TTCGACACCT GTGTGAGGAA 
GCCGACATCA GGTGTGGCAT AGATGAGATC TCGGTGCACC ATTTCTTGCC AAACTATAAC 
ACCTTCATGG AGTTCGCATC TGTCAGCAAC ATGATGTCGA CAGGAAGAGC AGCTCTTCAG 
AAAAGAGTGA TGGCCCTGCT CAGGCGCATC GAACACCCCA CCGCTGGAAA CACCGAGGCT 
TGGGAAGATA CTTACGCCAA ATGGGAGCAA GCTACAAAAT CAATCCTCAA TTATCCGAAA 
GCCAAAATTG AAGATGGCCA GGTTGCTGAG AGTCTTCACA AGACCATTGT GAAGAGAAGA 
ATGTCTCACG TGAGTGGAGG GGGCTCCATA GACTTGTGCG ACACTGACTC GCTACAGGAG 
TGGATCAACA TGACTGGGTT TCTCTGTGCC CTTGGTGGCG TGTGCCTACA GCAGCGGAGC 
AATGCCGGCT TTGCCACCTA CAGCCCTCCC ATGGGCCCTA TCAGTGAACG CAAGGGCTCT 
CTCATTTCCA TGGTGTCCAG CGATGGAAAC GCAGAGACGC CTGTCAGCAA GTTCATGGAT 
CGGCTGCTCT CCTTGATGGT GTGTAACCAC GAAAAAGTGG GGCTGCAGAT ACGAAGCAAT 
GTCAAGGACT TGTTGGGTCT TGAATTGAGT CCTGCCCTTT ATCCAATGCT GTTTAACAAA 
TTAAAAAATA CCATCAGCAA GTTTTTTGAT CCTCAAGGAC AGGTTTTATT GACTGACACC 
AACACCCAGT TTGTGGAGCA GACCATAGCC ATAATGAAGA ACTTGCTTGA TAATCATGCT 
GAGGGTAGTT CTGAACACAT GGGACAAGCT AGCATCGAAA CTATGATGTT AAATCTGGTC 
AGATATGTTA GAGTGTTTGG GAATTTGGTG CAGGCAATTC AGATCAAAAC TAAGCTGTGC 
CAGCTGGTTC AAGTAATGAT GGAGAGGAGA GATGACCTTT CATTTTGCCA AGAGATGAAA 
TTTAGGAATA AAATGGTAGA ATATCTGACA GATTGGGTCA TGGGAACATC TAACCAAGCT 
GCAGATGAAG ATGTGAAATG TCTAACTAGA GATTTGGACC AGGCAAGCAT GGAAGCAGTG 
GTGTCATTGC TCGCTGGGCT CCCTCTGCAG CCTGAGGAAG GCGATGGGGT GGAATTGATG 
GAAGCCAAAT CCCAGCTGTT CCTTAAGTAT TTCACCCTCT TTATGAACCT TCTTAATGAC 
TGCAGTGAGG TCGAAGAAGA AAATGTCCAG ATTGGGGGCA GGAAGCGCGG CATGTCGCGA 
AGGCTGGCGT CCCTGCGCCA CTGCACCGTG CTGGCAATGT CCAACCTGCT CAATGCGAAT 
GTAGACAGCG GCCTGATGCA CTCTATAGTG CTAGGATTGG GTTACCACAA AGATCTTCAG 
ACAAGAGCCA CCTTCATGGA AGTCCTCACA AAAATCCTTC AGCAAGGCAC AGAGTTTGAT 
ACGCTCGCAG AAACCGTGTT AGCTGATCGA TTTGAGAGAC TTGTTGAGTT GGTCACGATG 
ATGGGTGATC AAGGAGAGCT CCCCATAGCC ATGGCTCTGG CCAACGTGGT GCCTTGTTCT 
CAGTGGGATG AACTTGCTCG AGTCCTTGTT ACTCTCTTTG ATTCCCGACA CTTACTGTAT 
CAGCTCCTTT GGAACATGTT TTCTAAGGAA GTTGAGCTGG CAGACTCCAT GCAGACACTT 
TTTAGAGGAA ACAGCTTGGC CAGTAAAATC ATGACCTTCT GCTTTAAGGT CTACGGTGCT 
ACATACCTGC AGAAGCTTCT GGAGCCTTTA CTCCGCTTTG TGATCACATC CTCCGAATGG 
CAGCACGTCA GCTTCGAGGT CGATCCCACC AGGTTAGAGC ATACTGAGAG CCTCGAGGAC 
AACCAGCGGA ACCTTTTGCA AATGACTGAG AAGTTCTTCC ACGCCATCAT CAGTTCTTCC 
TCCGAGTTCC CCCCTCAGCT ACGCAGTGTG TGCCACTGCC TGTACCAGGC AACTTGCCAC 
TCTCTACTGA ATAAAGCTAC CGTAAAAGAA AAAAAGGAAA ACAAAAAATC AGTTGTTAGC 
CAGAGATTCC CTCAGAACAG CATTGGCGCC GTGGGGAGTG CCATGTTCCT GAGATTCATC 
AACCCTGCCG TTGTCTCTCC CTACGAGGCA GGGATTTTAG AGAGAAAACC GCTCCCTAGA 
ATTGAAAGGG GGTTGAAGCT GATGTCAAAG ATTCTTCAGA GCATTGCCAA TCATGTCCTT 
TTCACAAAAG AAGAGTATAT GAGGCCTTTT AACGATTTTG TGAAAAGCAA CTTTGATGTA 
GCACGGAGAT TTTTCCTTGA TATCGCATCT GATTGTCCTG TAAGTGATGC CATAAACCAC 
AGCCTTTCCT TCATCAGCGA TGGCAATGTC TTGGCTTTGC ACCGTCTGCT CTGGAACAAC 
CAAGAGAAAA TCGGCCAATA TCTTTCAAGC AACAGAGATC ACAAAGCTGT TGGAAGGCGA 
CCTTTCGATA AGATGGCAAC ACTGCTTGCC TACCTGGGAC CTCCAGAGCA CAAGCCTGTG 
GCTGACACAC ACTGGTCCAG CCTAAATCTT ACCAGCTCCA AGTTTGAGGA ATTCATGACA 
AGGCACCAGG TACATGAAAA AGAGGAATTC AAGGCTCTGA AAACATTAAA TATCTTCTAT 
CAAGCAGGCA CTTCCAAAGC TGGAAATCCT GTTTTTTATT ATGTCACACG AAGGTTCAAG 
ACTGGCCAGA TCAACGGGGA TTTGCTTATA TACCACGTCT TACTAACTTT AAAGCCATAT 
TATGCAAAGC CATATGAGAT TGTAATGGAC CTCACTCATG CCGGGCCTAG CAATCGCTTT 
AAGACAGACT TCCTCTCCAA GTGGTTTGTT GTTTTCCCGG GCTTTGCTTA CGAGAACGTC 
TCTTCGGTCT ACATCTACAA CTGTAATTCT TGGGTTCGGG AGTACACCAA ATACCATGAG 
AGACTGCTCA CGGGACTCAA AGGCAGCAAA AGACTCTTTT TCATAGACTC TCCCTCAAAA 
CTGGCTGAGC ATATCGATTA TGATCAGCAA AAACTGCCAG CCGCGACCTT GGCTCTGGAT 
GAAGACCTCA AGGTGTACAA CAGTGCTCTC AAGCTGGCTC ACAAGGACAC CAAGGTGACC 
CTCAAGGTTG GCTCTACTGC GATCCAAGTG ACGTCATCTG AGCGAACTAA AGTCCTGGGA 
CAGACGGTTT TTCTGAATGA TATTTACTAT GCCTCTGAGA TCGAAGAAAT CTGTCTTGTG 
GATGAAAACC AGTTCACTCT GACCATTTCC AATCAGGGCA CTCCCCTCAC CTTCATGCAT 
CAAGAGTGTG AAGGCATTGT GGCGTCCATC ATTCACATCC GAACTCGATG GGAGCTGTCC 
CAGCCAGATT CTATCCCCCA GCACACTAAG ATCCGGCCAA AGGACGTTCC CGGAACACTT 
TTGAATATCG CATTACTTAA CTTGGGTAGT TCGGACCCAA GTTTACGGTC AGCCGCCTAT 
AATCTTCTCT GTGCCTTAAC CTGTACCTTT AATTTAAAAA TTGAGGGCCA ATTACTAGAA 
ACATCAGGTC TGTGTATACC TGCCAACAAT ACCCTTTTTA TTGTCTCTAT TAGTAAAACC 
CTGGCAGCTA ATGAGCCACA CCTCACCTTA GAGTTTTTGG AAGAGTGCAT TTCCGGATTT 
AGCAAATCCA GTATTGAACT GAAGCACCTG TGTCTAGAAT ACATGACTCC CTGGCTATCA 
AATCTAGTCC GTTTCTGTAA ACATAATGAT GATGCCAAAC GACAGAGAGT CACTGCTATT 
CTTGATAAAC TTATAACAAT GACCATAAAT GAAAAGCAGA TGTACCCATC GATCCAAGCC 
AAGATATGGG GAAGCCTTGG GCAGATTACA GATCTGCTCG ATGTAGTGCT GGACAGCTTT 
ATCAAAACCA GTGCCACAGG TGGTTTAGGA TCCATCAAGG CTGAAGTGAT GGCTGACACG 
GCCGTGGCTC TGGCTTCTGG GAACGTCAAA CTAGTTTCGA GCAAGGTGAT TGGAAGAATG 
TGCAAAATCA TTGACAAGAC CTGCCTCTCT CCAACTCCTA CCTTAGAGCA GCACCTCATG 
TGGGATGATA TCGCCATTTT AGCACGCTAC ATGCTAATGC TGTCCTTCAA TAATTCTCTG 
GATGTGGCGG CTCATCTTCC CTACCTCTTC CATGTGGTGA CTCTGTTGGT GGCCACAGGT 
CCCTTGTCCC TTCGTGCTTC CACACACGGA CTGGTCATCA ATATCATCCA TTCTCTGTGC 
ACGTGCTCAC AGCTTCACTT TAGTGAAGAG ACCAAGCAAG TTTTACGACT GAGTCTGACT 
GAGTTCTCAC TACCTAAGTT TTATTTGCTG TTCGGCATCA GCAAAGTCAA ATCAGCTGCC 
GTGATAGCCT TCCGGTCCAG TTACCGGGAC AGGTCTTTCT CCCCTGGCTC CTATGAGAGG 
GAGACCTTTG CCCTGGCCTC CTTGGAAACA GTCACAGAAG CTCTCTTGGA GATCATGGAG 
GCTTGTATGA GAGATATTCC TGCATGCAAG TGGCTGGACC AGTGGACAGA GCTAGCCCAG 
AGGTTTGCGT TCCAGTACAA TCCCTCCCTG CAGCCACGAG CTCTGGTTGT GTTTGGTTGT 
ATCAGCAAAA GAGTGTCCCA TGGGCAGATC AAGCAGATTA TCCGCATTCT TAGCAAGGGA 
CTTGAGAGCT GCCTAAAAGG ACCTGATAAT TATAACAGTC AAGTTCTGAT TGAAGCTACC 
GTGATAGCTC TAACCAAATT ACAACCACTT CTTAATAAGC AGGAGTCCCC TCTGCACCGA 
GCTCTCTTCT GGGTGGCCGT TGCCGTGCTG CAGCTGGATG AAGTGGACCT GTACTCGGCG 
GGCACTGCCC TCCTCGAACA GAACCTGCAC ACCTTAGACA GCCTCCTGCA CATCTTCAAC 
GACCAGAGCC CAGAAGAAGT GTTCATGGAG ATCCGGCAGC CTCTGGAGTG GCACTGCAAG 
CAGATGGACC ACTTTGTGGG GCTCAACTTT AACTCCAGCT TTAACTTTGC CCTGGTCGGG 
CACCTGCTGA AGGGATTTCG GCATCCTTCA CCTGTCACTG TTGCAAGAAC TGTGAGGATT 
TTGCACACCC TACTAGCATT GGTTAACAAG CATAGGAATT GTGACAAGTT TGAGGTGAAC 
ACCCAGAGTG TGGCTTACTT AGCAGCTTTA CTTACTGTGT CTGAGGAGGT TCGAAGTCGC 
TGCAGCTTAA AGCATAGGAA ATCACTCCTA CTAACTGATA TCACAATGGA AAATGTGCCT 
ATGGATATAT ATCCCATCTA TCACAGTGAC CCCAGCCTTA GGACACTAAA AGAAACACAA 
CCGTGGTCCT CTCCAAAGGG ATCTGAGAGA CATCTTGCAG CCAGCTACCC TCCTGTGGGC 
CAAACTAGCC CCCGAACCAG AAAATCCATG AGCCTGGATA TGGGGCAGCC TTCCCAGGCC 
AACACAAAGA AGTTGCTTGG GACAAGGAAA AGCTTCGATC ATTTGATCTC TGATGCCAAA 
GCTCCCAAAA GGCAAGAACA GGAGTCTGGA GTCACCACGC CCCCCAAGAT GCGGAGAGTG 
GCCGAGAACG ACTACGAGAT GGAAACCCAG AGAATCTCCC CTCAGCCACA CCCGCCTCTG 
CGGAAAGTGT CCGTGTCCGA ATCCAATGTG CTGCTGGACG AGGAAGTGCT CACAGACCCC 
AAAACCCAGG CTCTTCTTCT CACTGTCCTG GCTACTCTGG TGAAGTACAC TGCGGACGAG 
TTTGACCAGC GCATTCTCTA TGAGTACTTA GCGGAGGCCA GTGTTGTCTT CCCAAAGGTT 
TTTCCAGTGG TACATAATTT GTTGGACTCA AAGATAAATA CTCTGCTCTC ATTATGCCAA 
GACCCAAATT TGCTAAACCC GGTTCATGGA ATTGTGCAGA GTGTGGTCTA CCACGAAGAG 
GCCCCGCCGC AGTACCAGAC GGCGTACCTC CAGAGTTTCG GTTTTAATGG TCTGTGGAGG 
TTTGCAGGAC CATTTTCTAA GACGCAGATC CCAGACTATG CCGAGCTCAT CGTGAAGTTT 
CTCGATGCCT TGATTGACAC GTATCTGCCT GGAATTGATG AGGAAGCCAG TGAGGAGTCC 
CTCCTGACAC CCACCTCTCC CTACCCGCCC GCGGTGCAGA GCCAGCTTAG CATCACTGCG 
AACCTCAACC TCTCTAACTC CATGACCTCA CTGGCAACTT CCCAGCATTC CCCAGGCATC 
GACAAGGAGA ATGTGGAGCT CTCGCCAACC ACCGGGCACT GCAGCGGCGG GAGGACGCGC 
CACGGCTCCG CCAGCCAGGT CCAGAAGCAA AGGAGTGCCG GCAGCTTCAA GAGGCACAGC 
ATCAAGAAGA TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967644.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823504.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X6, mRNA.

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CloneID	OSa234693
Clone ID Related Accession (Same CDS sequence)	XM_031967644.1
Accession Version	XM_031967644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGCAGTCA 
GATAGGAGTT CTTCCCATTT CCTCTACCTT TATGGGGCAG GCTATGATGT CTCTTCTGGT 
GGAGGTACCA GTCATATCTC TCTAGACCAC GAGGAGTTTA TGCGTACCTG TGCTCATGGA 
CCATCTCTCA GGAAAGTGAA AGGAAGCTCC TCAATGGACG GTGCAATCGG GTCCAGCGGG 
ACGCCCATCT GCCGCCAGGC CCAGACTAAG CTGGAGGTTG CTCTGTACAT GTTTCTGTGG 
AGTCCCGACA TTGAAGCCGT GCTCGTGGCC ATGTCCTGCT TTCGACACCT GTGTGAGGAA 
GCCGACATCA GGTGTGGCAT AGATGAGATC TCGGTGCACC ATTTCTTGCC AAACTATAAC 
ACCTTCATGG AGTTCGCATC TGTCAGCAAC ATGATGTCGA CAGGAAGAGC AGCTCTTCAG 
AAAAGAGTGA TGGCCCTGCT CAGGCGCATC GAACACCCCA CCGCTGGAAA CACCGAGGCT 
TGGGAAGATA CTTACGCCAA ATGGGAGCAA GCTACAAAAT CAATCCTCAA TTATCCGAAA 
GCCAAAATTG AAGATGGCCA GGTTGCTGAG AGTCTTCACA AGACCATTGT GAAGAGAAGA 
ATGTCTCACG TGAGTGGAGG GGGCTCCATA GACTTGTGCG ACACTGACTC GCTACAGGAG 
TGGATCAACA TGACTGGGTT TCTCTGTGCC CTTGGTGGCG TGTGCCTACA GCAGCGGAGC 
AATGCCGGCT TTGCCACCTA CAGCCCTCCC ATGGGCCCTA TCAGTGAACG CAAGGGCTCT 
CTCATTTCCA TGGTGTCCAG CGATGGAAAC GCAGAGACGC CTGTCAGCAA GTTCATGGAT 
CGGCTGCTCT CCTTGATGGT GTGTAACCAC GAAAAAGTGG GGCTGCAGAT ACGAAGCAAT 
GTCAAGGACT TGTTGGGTCT TGAATTGAGT CCTGCCCTTT ATCCAATGCT GTTTAACAAA 
TTAAAAAATA CCATCAGCAA GTTTTTTGAT CCTCAAGGAC AGGTTTTATT GACTGACACC 
AACACCCAGT TTGTGGAGCA GACCATAGCC ATAATGAAGA ACTTGCTTGA TAATCATGCT 
GAGGGTAGTT CTGAACACAT GGGACAAGCT AGCATCGAAA CTATGATGTT AAATCTGGTC 
AGATATGTTA GAGTGTTTGG GAATTTGGTG CAGGCAATTC AGATCAAAAC TAAGCTGTGC 
CAGCTGGTTC AAGTAATGAT GGAGAGGAGA GATGACCTTT CATTTTGCCA AGAGATGAAA 
TTTAGGAATA AAATGGTAGA ATATCTGACA GATTGGGTCA TGGGAACATC TAACCAAGCT 
GCAGATGAAG ATGTGAAATG TCTAACTAGA GATTTGGACC AGGCAAGCAT GGAAGCAGTG 
GTGTCATTGC TCGCTGGGCT CCCTCTGCAG CCTGAGGAAG GCGATGGGGT GGAATTGATG 
GAAGCCAAAT CCCAGCTGTT CCTTAAGTAT TTCACCCTCT TTATGAACCT TCTTAATGAC 
TGCAGTGAGG TCGAAGAAGA AAATGTCCAG ATTGGGGGCA GGAAGCGCGG CATGTCGCGA 
AGGCTGGCGT CCCTGCGCCA CTGCACCGTG CTGGCAATGT CCAACCTGCT CAATGCGAAT 
GTAGACAGCG GCCTGATGCA CTCTATAGGA TTGGGTTACC ACAAAGATCT TCAGACAAGA 
GCCACCTTCA TGGAAGTCCT CACAAAAATC CTTCAGCAAG GCACAGAGTT TGATACGCTC 
GCAGAAACCG TGTTAGCTGA TCGATTTGAG AGACTTGTTG AGTTGGTCAC GATGATGGGT 
GATCAAGGAG AGCTCCCCAT AGCCATGGCT CTGGCCAACG TGGTGCCTTG TTCTCAGTGG 
GATGAACTTG CTCGAGTCCT TGTTACTCTC TTTGATTCCC GACACTTACT GTATCAGCTC 
CTTTGGAACA TGTTTTCTAA GGAAGTTGAG CTGGCAGACT CCATGCAGAC ACTTTTTAGA 
GGAAACAGCT TGGCCAGTAA AATCATGACC TTCTGCTTTA AGGTCTACGG TGCTACATAC 
CTGCAGAAGC TTCTGGAGCC TTTACTCCGC TTTGTGATCA CATCCTCCGA ATGGCAGCAC 
GTCAGCTTCG AGGTCGATCC CACCAGGTTA GAGCATACTG AGAGCCTCGA GGACAACCAG 
CGGAACCTTT TGCAAATGAC TGAGAAGTTC TTCCACGCCA TCATCAGTTC TTCCTCCGAG 
TTCCCCCCTC AGCTACGCAG TGTGTGCCAC TGCCTGTACC AGGCAACTTG CCACTCTCTA 
CTGAATAAAG CTACCGTAAA AGAAAAAAAG GAAAACAAAA AATCAGTTGT TAGCCAGAGA 
TTCCCTCAGA ACAGCATTGG CGCCGTGGGG AGTGCCATGT TCCTGAGATT CATCAACCCT 
GCCGTTGTCT CTCCCTACGA GGCAGGGATT TTAGAGAGAA AACCGCTCCC TAGAATTGAA 
AGGGGGTTGA AGCTGATGTC AAAGATTCTT CAGAGCATTG CCAATCATGT CCTTTTCACA 
AAAGAAGAGT ATATGAGGCC TTTTAACGAT TTTGTGAAAA GCAACTTTGA TGTAGCACGG 
AGATTTTTCC TTGATATCGC ATCTGATTGT CCTGTAAGTG ATGCCATAAA CCACAGCCTT 
TCCTTCATCA GCGATGGCAA TGTCTTGGCT TTGCACCGTC TGCTCTGGAA CAACCAAGAG 
AAAATCGGCC AATATCTTTC AAGCAACAGA GATCACAAAG CTGTTGGAAG GCGACCTTTC 
GATAAGATGG CAACACTGCT TGCCTACCTG GGACCTCCAG AGCACAAGCC TGTGGCTGAC 
ACACACTGGT CCAGCCTAAA TCTTACCAGC TCCAAGTTTG AGGAATTCAT GACAAGGCAC 
CAGGTACATG AAAAAGAGGA ATTCAAGGCT CTGAAAACAT TAAATATCTT CTATCAAGCA 
GGCACTTCCA AAGCTGGAAA TCCTGTTTTT TATTATGTCA CACGAAGGTT CAAGACTGGC 
CAGATCAACG GGGATTTGCT TATATACCAC GTCTTACTAA CTTTAAAGCC ATATTATGCA 
AAGCCATATG AGATTGTAAT GGACCTCACT CATGCCGGGC CTAGCAATCG CTTTAAGACA 
GACTTCCTCT CCAAGTGGTT TGTTGTTTTC CCGGGCTTTG CTTACGAGAA CGTCTCTTCG 
GTCTACATCT ACAACTGTAA TTCTTGGGTT CGGGAGTACA CCAAATACCA TGAGAGACTG 
CTCACGGGAC TCAAAGGCAG CAAAAGACTC TTTTTCATAG ACTCTCCCTC AAAACTGGCT 
GAGCATATCG ATTATGATCA GCAAAAACTG CCAGCCGCGA CCTTGGCTCT GGATGAAGAC 
CTCAAGGTGT ACAACAGTGC TCTCAAGCTG GCTCACAAGG ACACCAAGGT GACCCTCAAG 
GTTGGCTCTA CTGCGATCCA AGTGACGTCA TCTGAGCGAA CTAAAGTCCT GGGACAGACG 
GTTTTTCTGA ATGATATTTA CTATGCCTCT GAGATCGAAG AAATCTGTCT TGTGGATGAA 
AACCAGTTCA CTCTGACCAT TTCCAATCAG GGCACTCCCC TCACCTTCAT GCATCAAGAG 
TGTGAAGGCA TTGTGGCGTC CATCATTCAC ATCCGAACTC GATGGGAGCT GTCCCAGCCA 
GATTCTATCC CCCAGCACAC TAAGATCCGG CCAAAGGACG TTCCCGGAAC ACTTTTGAAT 
ATCGCATTAC TTAACTTGGG TAGTTCGGAC CCAAGTTTAC GGTCAGCCGC CTATAATCTT 
CTCTGTGCCT TAACCTGTAC CTTTAATTTA AAAATTGAGG GCCAATTACT AGAAACATCA 
GGTCTGTGTA TACCTGCCAA CAATACCCTT TTTATTGTCT CTATTAGTAA AACCCTGGCA 
GCTAATGAGC CACACCTCAC CTTAGAGTTT TTGGAAGAGT GCATTTCCGG ATTTAGCAAA 
TCCAGTATTG AACTGAAGCA CCTGTGTCTA GAATACATGA CTCCCTGGCT ATCAAATCTA 
GTCCGTTTCT GTAAACATAA TGATGATGCC AAACGACAGA GAGTCACTGC TATTCTTGAT 
AAACTTATAA CAATGACCAT AAATGAAAAG CAGATGTACC CATCGATCCA AGCCAAGATA 
TGGGGAAGCC TTGGGCAGAT TACAGATCTG CTCGATGTAG TGCTGGACAG CTTTATCAAA 
ACCAGTGCCA CAGGTGGTTT AGGATCCATC AAGGCTGAAG TGATGGCTGA CACGGCCGTG 
GCTCTGGCTT CTGGGAACGT CAAACTAGTT TCGAGCAAGG TGATTGGAAG AATGTGCAAA 
ATCATTGACA AGACCTGCCT CTCTCCAACT CCTACCTTAG AGCAGCACCT CATGTGGGAT 
GATATCGCCA TTTTAGCACG CTACATGCTA ATGCTGTCCT TCAATAATTC TCTGGATGTG 
GCGGCTCATC TTCCCTACCT CTTCCATGTG GTGACTCTGT TGGTGGCCAC AGGTCCCTTG 
TCCCTTCGTG CTTCCACACA CGGACTGGTC ATCAATATCA TCCATTCTCT GTGCACGTGC 
TCACAGCTTC ACTTTAGTGA AGAGACCAAG CAAGTTTTAC GACTGAGTCT GACTGAGTTC 
TCACTACCTA AGTTTTATTT GCTGTTCGGC ATCAGCAAAG TCAAATCAGC TGCCGTGATA 
GCCTTCCGGT CCAGTTACCG GGACAGGTCT TTCTCCCCTG GCTCCTATGA GAGGGAGACC 
TTTGCCCTGG CCTCCTTGGA AACAGTCACA GAAGCTCTCT TGGAGATCAT GGAGGCTTGT 
ATGAGAGATA TTCCTGCATG CAAGTGGCTG GACCAGTGGA CAGAGCTAGC CCAGAGGTTT 
GCGTTCCAGT ACAATCCCTC CCTGCAGCCA CGAGCTCTGG TTGTGTTTGG TTGTATCAGC 
AAAAGAGTGT CCCATGGGCA GATCAAGCAG ATTATCCGCA TTCTTAGCAA GGGACTTGAG 
AGCTGCCTAA AAGGACCTGA TAATTATAAC AGTCAAGTTC TGATTGAAGC TACCGTGATA 
GCTCTAACCA AATTACAACC ACTTCTTAAT AAGCAGGAGT CCCCTCTGCA CCGAGCTCTC 
TTCTGGGTGG CCGTTGCCGT GCTGCAGCTG GATGAAGTGG ACCTGTACTC GGCGGGCACT 
GCCCTCCTCG AACAGAACCT GCACACCTTA GACAGCCTCC TGCACATCTT CAACGACCAG 
AGCCCAGAAG AAGTGTTCAT GGAGATCCGG CAGCCTCTGG AGTGGCACTG CAAGCAGATG 
GACCACTTTG TGGGGCTCAA CTTTAACTCC AGCTTTAACT TTGCCCTGGT CGGGCACCTG 
CTGAAGGGAT TTCGGCATCC TTCACCTGTC ACTGTTGCAA GAACTGTGAG GATTTTGCAC 
ACCCTACTAG CATTGGTTAA CAAGCATAGG AATTGTGACA AGTTTGAGGT GAACACCCAG 
AGTGTGGCTT ACTTAGCAGC TTTACTTACT GTGTCTGAGG AGGTTCGAAG TCGCTGCAGC 
TTAAAGCATA GGAAATCACT CCTACTAACT GATATCACAA TGGAAAATGT GCCTATGGAT 
ATATATCCCA TCTATCACAG TGACCCCAGC CTTAGGACAC TAAAAGAAAC ACAACCGTGG 
TCCTCTCCAA AGGGATCTGA GAGACATCTT GCAGCCAGCT ACCCTCCTGT GGGCCAAACT 
AGCCCCCGAA CCAGAAAATC CATGAGCCTG GATATGGGGC AGCCTTCCCA GGCCAACACA 
AAGAAGTTGC TTGGGACAAG GAAAAGCTTC GATCATTTGA TCTCTGATGC CAAAGCTCCC 
AAAAGGCAAG AACAGGAGTC TGGAGTCACC ACGCCCCCCA AGATGCGGAG AGTGGCCGAG 
AACGACTACG AGATGGAAAC CCAGAGAATC TCCCCTCAGC CACACCCGCC TCTGCGGAAA 
GTGTCCGTGT CCGAATCCAA TGTGCTGCTG GACGAGGAAG TGCTCACAGA CCCCAAAACC 
CAGGCTCTTC TTCTCACTGT CCTGGCTACT CTGGTGAAGT ACACTGCGGA CGAGTTTGAC 
CAGCGCATTC TCTATGAGTA CTTAGCGGAG GCCAGTGTTG TCTTCCCAAA GGTTTTTCCA 
GTGGTACATA ATTTGTTGGA CTCAAAGATA AATACTCTGC TCTCATTATG CCAAGACCCA 
AATTTGCTAA ACCCGGTTCA TGGAATTGTG CAGAGTGTGG TCTACCACGA AGAGGCCCCG 
CCGCAGTACC AGACGGCGTA CCTCCAGAGT TTCGGTTTTA ATGGTCTGTG GAGGTTTGCA 
GGACCATTTT CTAAGCAGAC GCAGATCCCA GACTATGCCG AGCTCATCGT GAAGTTTCTC 
GATGCCTTGA TTGACACGTA TCTGCCTGGA ATTGATGAGG AAGCCAGTGA GGAGTCCCTC 
CTGACACCCA CCTCTCCCTA CCCGCCCGCG GTGCAGAGCC AGCTTAGCAT CACTGCGAAC 
CTCAACCTCT CTAACTCCAT GACCTCACTG GCAACTTCCC AGCATTCCCC AGGCATCGAC 
AAGGAGAATG TGGAGCTCTC GCCAACCACC GGGCACTGCA GCGGCGGGAG GACGCGCCAC 
GGCTCCGCCA GCCAGGTCCA GAAGCAAAGG AGTGCCGGCA GCTTCAAGAG GCACAGCATC 
AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823504.1
CDS	9..8663
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGVSDLQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKFSTLLTTITAWILSPWLCDTDVSIPLSCQITRPQSSFKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKQESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967644.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967644.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGCAGTCA 
GATAGGAGTT CTTCCCATTT CCTCTACCTT TATGGGGCAG GCTATGATGT CTCTTCTGGT 
GGAGGTACCA GTCATATCTC TCTAGACCAC GAGGAGTTTA TGCGTACCTG TGCTCATGGA 
CCATCTCTCA GGAAAGTGAA AGGAAGCTCC TCAATGGACG GTGCAATCGG GTCCAGCGGG 
ACGCCCATCT GCCGCCAGGC CCAGACTAAG CTGGAGGTTG CTCTGTACAT GTTTCTGTGG 
AGTCCCGACA TTGAAGCCGT GCTCGTGGCC ATGTCCTGCT TTCGACACCT GTGTGAGGAA 
GCCGACATCA GGTGTGGCAT AGATGAGATC TCGGTGCACC ATTTCTTGCC AAACTATAAC 
ACCTTCATGG AGTTCGCATC TGTCAGCAAC ATGATGTCGA CAGGAAGAGC AGCTCTTCAG 
AAAAGAGTGA TGGCCCTGCT CAGGCGCATC GAACACCCCA CCGCTGGAAA CACCGAGGCT 
TGGGAAGATA CTTACGCCAA ATGGGAGCAA GCTACAAAAT CAATCCTCAA TTATCCGAAA 
GCCAAAATTG AAGATGGCCA GGTTGCTGAG AGTCTTCACA AGACCATTGT GAAGAGAAGA 
ATGTCTCACG TGAGTGGAGG GGGCTCCATA GACTTGTGCG ACACTGACTC GCTACAGGAG 
TGGATCAACA TGACTGGGTT TCTCTGTGCC CTTGGTGGCG TGTGCCTACA GCAGCGGAGC 
AATGCCGGCT TTGCCACCTA CAGCCCTCCC ATGGGCCCTA TCAGTGAACG CAAGGGCTCT 
CTCATTTCCA TGGTGTCCAG CGATGGAAAC GCAGAGACGC CTGTCAGCAA GTTCATGGAT 
CGGCTGCTCT CCTTGATGGT GTGTAACCAC GAAAAAGTGG GGCTGCAGAT ACGAAGCAAT 
GTCAAGGACT TGTTGGGTCT TGAATTGAGT CCTGCCCTTT ATCCAATGCT GTTTAACAAA 
TTAAAAAATA CCATCAGCAA GTTTTTTGAT CCTCAAGGAC AGGTTTTATT GACTGACACC 
AACACCCAGT TTGTGGAGCA GACCATAGCC ATAATGAAGA ACTTGCTTGA TAATCATGCT 
GAGGGTAGTT CTGAACACAT GGGACAAGCT AGCATCGAAA CTATGATGTT AAATCTGGTC 
AGATATGTTA GAGTGTTTGG GAATTTGGTG CAGGCAATTC AGATCAAAAC TAAGCTGTGC 
CAGCTGGTTC AAGTAATGAT GGAGAGGAGA GATGACCTTT CATTTTGCCA AGAGATGAAA 
TTTAGGAATA AAATGGTAGA ATATCTGACA GATTGGGTCA TGGGAACATC TAACCAAGCT 
GCAGATGAAG ATGTGAAATG TCTAACTAGA GATTTGGACC AGGCAAGCAT GGAAGCAGTG 
GTGTCATTGC TCGCTGGGCT CCCTCTGCAG CCTGAGGAAG GCGATGGGGT GGAATTGATG 
GAAGCCAAAT CCCAGCTGTT CCTTAAGTAT TTCACCCTCT TTATGAACCT TCTTAATGAC 
TGCAGTGAGG TCGAAGAAGA AAATGTCCAG ATTGGGGGCA GGAAGCGCGG CATGTCGCGA 
AGGCTGGCGT CCCTGCGCCA CTGCACCGTG CTGGCAATGT CCAACCTGCT CAATGCGAAT 
GTAGACAGCG GCCTGATGCA CTCTATAGGA TTGGGTTACC ACAAAGATCT TCAGACAAGA 
GCCACCTTCA TGGAAGTCCT CACAAAAATC CTTCAGCAAG GCACAGAGTT TGATACGCTC 
GCAGAAACCG TGTTAGCTGA TCGATTTGAG AGACTTGTTG AGTTGGTCAC GATGATGGGT 
GATCAAGGAG AGCTCCCCAT AGCCATGGCT CTGGCCAACG TGGTGCCTTG TTCTCAGTGG 
GATGAACTTG CTCGAGTCCT TGTTACTCTC TTTGATTCCC GACACTTACT GTATCAGCTC 
CTTTGGAACA TGTTTTCTAA GGAAGTTGAG CTGGCAGACT CCATGCAGAC ACTTTTTAGA 
GGAAACAGCT TGGCCAGTAA AATCATGACC TTCTGCTTTA AGGTCTACGG TGCTACATAC 
CTGCAGAAGC TTCTGGAGCC TTTACTCCGC TTTGTGATCA CATCCTCCGA ATGGCAGCAC 
GTCAGCTTCG AGGTCGATCC CACCAGGTTA GAGCATACTG AGAGCCTCGA GGACAACCAG 
CGGAACCTTT TGCAAATGAC TGAGAAGTTC TTCCACGCCA TCATCAGTTC TTCCTCCGAG 
TTCCCCCCTC AGCTACGCAG TGTGTGCCAC TGCCTGTACC AGGCAACTTG CCACTCTCTA 
CTGAATAAAG CTACCGTAAA AGAAAAAAAG GAAAACAAAA AATCAGTTGT TAGCCAGAGA 
TTCCCTCAGA ACAGCATTGG CGCCGTGGGG AGTGCCATGT TCCTGAGATT CATCAACCCT 
GCCGTTGTCT CTCCCTACGA GGCAGGGATT TTAGAGAGAA AACCGCTCCC TAGAATTGAA 
AGGGGGTTGA AGCTGATGTC AAAGATTCTT CAGAGCATTG CCAATCATGT CCTTTTCACA 
AAAGAAGAGT ATATGAGGCC TTTTAACGAT TTTGTGAAAA GCAACTTTGA TGTAGCACGG 
AGATTTTTCC TTGATATCGC ATCTGATTGT CCTGTAAGTG ATGCCATAAA CCACAGCCTT 
TCCTTCATCA GCGATGGCAA TGTCTTGGCT TTGCACCGTC TGCTCTGGAA CAACCAAGAG 
AAAATCGGCC AATATCTTTC AAGCAACAGA GATCACAAAG CTGTTGGAAG GCGACCTTTC 
GATAAGATGG CAACACTGCT TGCCTACCTG GGACCTCCAG AGCACAAGCC TGTGGCTGAC 
ACACACTGGT CCAGCCTAAA TCTTACCAGC TCCAAGTTTG AGGAATTCAT GACAAGGCAC 
CAGGTACATG AAAAAGAGGA ATTCAAGGCT CTGAAAACAT TAAATATCTT CTATCAAGCA 
GGCACTTCCA AAGCTGGAAA TCCTGTTTTT TATTATGTCA CACGAAGGTT CAAGACTGGC 
CAGATCAACG GGGATTTGCT TATATACCAC GTCTTACTAA CTTTAAAGCC ATATTATGCA 
AAGCCATATG AGATTGTAAT GGACCTCACT CATGCCGGGC CTAGCAATCG CTTTAAGACA 
GACTTCCTCT CCAAGTGGTT TGTTGTTTTC CCGGGCTTTG CTTACGAGAA CGTCTCTTCG 
GTCTACATCT ACAACTGTAA TTCTTGGGTT CGGGAGTACA CCAAATACCA TGAGAGACTG 
CTCACGGGAC TCAAAGGCAG CAAAAGACTC TTTTTCATAG ACTCTCCCTC AAAACTGGCT 
GAGCATATCG ATTATGATCA GCAAAAACTG CCAGCCGCGA CCTTGGCTCT GGATGAAGAC 
CTCAAGGTGT ACAACAGTGC TCTCAAGCTG GCTCACAAGG ACACCAAGGT GACCCTCAAG 
GTTGGCTCTA CTGCGATCCA AGTGACGTCA TCTGAGCGAA CTAAAGTCCT GGGACAGACG 
GTTTTTCTGA ATGATATTTA CTATGCCTCT GAGATCGAAG AAATCTGTCT TGTGGATGAA 
AACCAGTTCA CTCTGACCAT TTCCAATCAG GGCACTCCCC TCACCTTCAT GCATCAAGAG 
TGTGAAGGCA TTGTGGCGTC CATCATTCAC ATCCGAACTC GATGGGAGCT GTCCCAGCCA 
GATTCTATCC CCCAGCACAC TAAGATCCGG CCAAAGGACG TTCCCGGAAC ACTTTTGAAT 
ATCGCATTAC TTAACTTGGG TAGTTCGGAC CCAAGTTTAC GGTCAGCCGC CTATAATCTT 
CTCTGTGCCT TAACCTGTAC CTTTAATTTA AAAATTGAGG GCCAATTACT AGAAACATCA 
GGTCTGTGTA TACCTGCCAA CAATACCCTT TTTATTGTCT CTATTAGTAA AACCCTGGCA 
GCTAATGAGC CACACCTCAC CTTAGAGTTT TTGGAAGAGT GCATTTCCGG ATTTAGCAAA 
TCCAGTATTG AACTGAAGCA CCTGTGTCTA GAATACATGA CTCCCTGGCT ATCAAATCTA 
GTCCGTTTCT GTAAACATAA TGATGATGCC AAACGACAGA GAGTCACTGC TATTCTTGAT 
AAACTTATAA CAATGACCAT AAATGAAAAG CAGATGTACC CATCGATCCA AGCCAAGATA 
TGGGGAAGCC TTGGGCAGAT TACAGATCTG CTCGATGTAG TGCTGGACAG CTTTATCAAA 
ACCAGTGCCA CAGGTGGTTT AGGATCCATC AAGGCTGAAG TGATGGCTGA CACGGCCGTG 
GCTCTGGCTT CTGGGAACGT CAAACTAGTT TCGAGCAAGG TGATTGGAAG AATGTGCAAA 
ATCATTGACA AGACCTGCCT CTCTCCAACT CCTACCTTAG AGCAGCACCT CATGTGGGAT 
GATATCGCCA TTTTAGCACG CTACATGCTA ATGCTGTCCT TCAATAATTC TCTGGATGTG 
GCGGCTCATC TTCCCTACCT CTTCCATGTG GTGACTCTGT TGGTGGCCAC AGGTCCCTTG 
TCCCTTCGTG CTTCCACACA CGGACTGGTC ATCAATATCA TCCATTCTCT GTGCACGTGC 
TCACAGCTTC ACTTTAGTGA AGAGACCAAG CAAGTTTTAC GACTGAGTCT GACTGAGTTC 
TCACTACCTA AGTTTTATTT GCTGTTCGGC ATCAGCAAAG TCAAATCAGC TGCCGTGATA 
GCCTTCCGGT CCAGTTACCG GGACAGGTCT TTCTCCCCTG GCTCCTATGA GAGGGAGACC 
TTTGCCCTGG CCTCCTTGGA AACAGTCACA GAAGCTCTCT TGGAGATCAT GGAGGCTTGT 
ATGAGAGATA TTCCTGCATG CAAGTGGCTG GACCAGTGGA CAGAGCTAGC CCAGAGGTTT 
GCGTTCCAGT ACAATCCCTC CCTGCAGCCA CGAGCTCTGG TTGTGTTTGG TTGTATCAGC 
AAAAGAGTGT CCCATGGGCA GATCAAGCAG ATTATCCGCA TTCTTAGCAA GGGACTTGAG 
AGCTGCCTAA AAGGACCTGA TAATTATAAC AGTCAAGTTC TGATTGAAGC TACCGTGATA 
GCTCTAACCA AATTACAACC ACTTCTTAAT AAGCAGGAGT CCCCTCTGCA CCGAGCTCTC 
TTCTGGGTGG CCGTTGCCGT GCTGCAGCTG GATGAAGTGG ACCTGTACTC GGCGGGCACT 
GCCCTCCTCG AACAGAACCT GCACACCTTA GACAGCCTCC TGCACATCTT CAACGACCAG 
AGCCCAGAAG AAGTGTTCAT GGAGATCCGG CAGCCTCTGG AGTGGCACTG CAAGCAGATG 
GACCACTTTG TGGGGCTCAA CTTTAACTCC AGCTTTAACT TTGCCCTGGT CGGGCACCTG 
CTGAAGGGAT TTCGGCATCC TTCACCTGTC ACTGTTGCAA GAACTGTGAG GATTTTGCAC 
ACCCTACTAG CATTGGTTAA CAAGCATAGG AATTGTGACA AGTTTGAGGT GAACACCCAG 
AGTGTGGCTT ACTTAGCAGC TTTACTTACT GTGTCTGAGG AGGTTCGAAG TCGCTGCAGC 
TTAAAGCATA GGAAATCACT CCTACTAACT GATATCACAA TGGAAAATGT GCCTATGGAT 
ATATATCCCA TCTATCACAG TGACCCCAGC CTTAGGACAC TAAAAGAAAC ACAACCGTGG 
TCCTCTCCAA AGGGATCTGA GAGACATCTT GCAGCCAGCT ACCCTCCTGT GGGCCAAACT 
AGCCCCCGAA CCAGAAAATC CATGAGCCTG GATATGGGGC AGCCTTCCCA GGCCAACACA 
AAGAAGTTGC TTGGGACAAG GAAAAGCTTC GATCATTTGA TCTCTGATGC CAAAGCTCCC 
AAAAGGCAAG AACAGGAGTC TGGAGTCACC ACGCCCCCCA AGATGCGGAG AGTGGCCGAG 
AACGACTACG AGATGGAAAC CCAGAGAATC TCCCCTCAGC CACACCCGCC TCTGCGGAAA 
GTGTCCGTGT CCGAATCCAA TGTGCTGCTG GACGAGGAAG TGCTCACAGA CCCCAAAACC 
CAGGCTCTTC TTCTCACTGT CCTGGCTACT CTGGTGAAGT ACACTGCGGA CGAGTTTGAC 
CAGCGCATTC TCTATGAGTA CTTAGCGGAG GCCAGTGTTG TCTTCCCAAA GGTTTTTCCA 
GTGGTACATA ATTTGTTGGA CTCAAAGATA AATACTCTGC TCTCATTATG CCAAGACCCA 
AATTTGCTAA ACCCGGTTCA TGGAATTGTG CAGAGTGTGG TCTACCACGA AGAGGCCCCG 
CCGCAGTACC AGACGGCGTA CCTCCAGAGT TTCGGTTTTA ATGGTCTGTG GAGGTTTGCA 
GGACCATTTT CTAAGCAGAC GCAGATCCCA GACTATGCCG AGCTCATCGT GAAGTTTCTC 
GATGCCTTGA TTGACACGTA TCTGCCTGGA ATTGATGAGG AAGCCAGTGA GGAGTCCCTC 
CTGACACCCA CCTCTCCCTA CCCGCCCGCG GTGCAGAGCC AGCTTAGCAT CACTGCGAAC 
CTCAACCTCT CTAACTCCAT GACCTCACTG GCAACTTCCC AGCATTCCCC AGGCATCGAC 
AAGGAGAATG TGGAGCTCTC GCCAACCACC GGGCACTGCA GCGGCGGGAG GACGCGCCAC 
GGCTCCGCCA GCCAGGTCCA GAAGCAAAGG AGTGCCGGCA GCTTCAAGAG GCACAGCATC 
AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967645.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823505.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X7, mRNA.

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CloneID	OSa234694
Clone ID Related Accession (Same CDS sequence)	XM_031967645.1
Accession Version	XM_031967645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGCAGCCA AAGGACACGA TGAGGTTAGA TGAAACAATG 
CTGGTCAAAC AGTTGCTGCC GGAAATCTGC CATTTCCTTC ACACCTACCG GGAAGGGAAC 
CAGCACGCAG CCGAGCTTCG GAGCTCCGCC TCTGGCGTCC TTTTCTCCCT CAGCTGTAAT 
AACTTCAATG CCGTCTTCAG CCGGATTTCC ACCAGGTTAC AGGAATTGAC TGTTTGCTCA 
GAAGATAATG TTGATGTCCA TGATATAGAA TTATTACAAT ATATCAATGT GGATTGTGCA 
AAACTAAAGC GGCTCTTGCA GGAAACTGTA TTTAAGTTTA AAGCCTTAAA GAAGGTAGCT 
CAACTAGCAG TTATAAATAG CCTAGAAAAG GCATTTTGGA ATTGGGTAGA GAACTATCCT 
GATGAATTTA CAAAGCTGTA CCAGACTCCC CAGACAGATA TGGCGGAATG TGCAGAAAAA 
TTATTTGACT TGGTGGATGG TTTTGCTGAA AGCACCAAAC GTAAAGCTGC AGTTTGGCCA 
CTGCAAATCA TTCTTCTTGT CTTGTGTCCA GAAATAATCC AGGACATTTC CAAGGATGTG 
GTTGATGAAG CGAAAATGAA TAAGTTTTCT ACACTGTTGA CCACTATCAC CGCCTGGATA 
CTTTCTCCTT GGCTTTGTGA TACCGATGTC TCAATTCCTC TTTCCTGCCA AATCACTCGT 
CCTCAGTCCT CCTTTAAAAT GTTTCTCGAC AATCTCAGAA GAGCGCTGGC CGGCCACAGT 
GGGAGCAGGC AGCTGACAGA GAGTGCCGCG ATCGCTTGTG TTAAACTGTG CAAAGCCAGC 
ACCTACATCA ACTGGGAGGA TAACTCGGTC ATTTTCCTGC TCGTCCAATC CATGGTGGTG 
GATCTGAAGA ACTTGCTCTT TAATCCCAGT AAGCCCTTCT CCAGAGGCAG CCAGAATGCT 
GATGTGGACC TCATGATAGA CTGCCTGGTT TCTTGCTTTC GCATCAGCCC TCACAACAAC 
CAACACTTTA AGATCTGTTT GGCCCAGAAC TCCCCATCTA CATTTCATTA TGTGCTGGTA 
AACTCCCTCC ATCGAATTAT CACCAATAGC ACTTTCAAGC ATGGACTTGG CACTGCTTCC 
GCCCTGGATT GGTGGCCCAA GATTGATGCT GTGTATTGCC ATTCTGTAGA ACTTCGAAAC 
ATGTTTGGTG AAACGCTTCA CAAGGCCGTG CAGGGCTGTG GGCCTCACCC CGCGATACGA 
ATGGCGCCGA GTCTTACATT TAAAGAAAAA ATGACAAGCC TTAAATTTAA AGAAAAACCT 
ACGGACTTGG AAACAAGAAG TTATAAATAC CTCTTATTAT CCATGGTGAA ACTAATCCAT 
GCAGATCCAA AGCTGTTGCT ATGTAATCCA AGAAAACAAG GGCCTGAAAC TCAGGGGAGT 
ACAGCAGAGT TAATCACAGG CCTTGTGCAG CTCGTCCCAC AGTCCAGCAT GCCAGAAATA 
GCTCAAGAAG CAATGGAGGC TTTGCTGGTT CTTCATCAGT TGGATAGTAT TGACTTATGG 
AATCCAGATT CACCTATAGA AACATTCTGG GAGATTAGCT CACAAATGCT TTTTTATATC 
TGTAAAAAGC TAACTACCCA TCAGATGCTT AGCAGTACAG AAATTCTCAA GTGGTTGCGA 
GAAATTTTGA TCTGTAGGAA CAAATATCTT CTTAAAAATA AGCAGTCAGA TAGGAGTTCT 
TCCCATTTCC TCTACCTTTA TGGGGCAGGC TATGATGTCT CTTCTGGTGG AGGTACCAGT 
CATATCTCTC TAGACCACGA GGAGTTTATG CGTACCTGTG CTCATGGACC ATCTCTCAGG 
AAAGTGAAAG GAAGCTCCTC AATGGACGGT GCAATCGGGT CCAGCGGGAC GCCCATCTGC 
CGCCAGGCCC AGACTAAGCT GGAGGTTGCT CTGTACATGT TTCTGTGGAG TCCCGACATT 
GAAGCCGTGC TCGTGGCCAT GTCCTGCTTT CGACACCTGT GTGAGGAAGC CGACATCAGG 
TGTGGCATAG ATGAGATCTC GGTGCACCAT TTCTTGCCAA ACTATAACAC CTTCATGGAG 
TTCGCATCTG TCAGCAACAT GATGTCGACA GGAAGAGCAG CTCTTCAGAA AAGAGTGATG 
GCCCTGCTCA GGCGCATCGA ACACCCCACC GCTGGAAACA CCGAGGCTTG GGAAGATACT 
TACGCCAAAT GGGAGCAAGC TACAAAATCA ATCCTCAATT ATCCGAAAGC CAAAATTGAA 
GATGGCCAGG TTGCTGAGAG TCTTCACAAG ACCATTGTGA AGAGAAGAAT GTCTCACGTG 
AGTGGAGGGG GCTCCATAGA CTTGTGCGAC ACTGACTCGC TACAGGAGTG GATCAACATG 
ACTGGGTTTC TCTGTGCCCT TGGTGGCGTG TGCCTACAGC AGCGGAGCAA TGCCGGCTTT 
GCCACCTACA GCCCTCCCAT GGGCCCTATC AGTGAACGCA AGGGCTCTCT CATTTCCATG 
GTGTCCAGCG ATGGAAACGC AGAGACGCCT GTCAGCAAGT TCATGGATCG GCTGCTCTCC 
TTGATGGTGT GTAACCACGA AAAAGTGGGG CTGCAGATAC GAAGCAATGT CAAGGACTTG 
TTGGGTCTTG AATTGAGTCC TGCCCTTTAT CCAATGCTGT TTAACAAATT AAAAAATACC 
ATCAGCAAGT TTTTTGATCC TCAAGGACAG GTTTTATTGA CTGACACCAA CACCCAGTTT 
GTGGAGCAGA CCATAGCCAT AATGAAGAAC TTGCTTGATA ATCATGCTGA GGGTAGTTCT 
GAACACATGG GACAAGCTAG CATCGAAACT ATGATGTTAA ATCTGGTCAG ATATGTTAGA 
GTGTTTGGGA ATTTGGTGCA GGCAATTCAG ATCAAAACTA AGCTGTGCCA GCTGGTTCAA 
GTAATGATGG AGAGGAGAGA TGACCTTTCA TTTTGCCAAG AGATGAAATT TAGGAATAAA 
ATGGTAGAAT ATCTGACAGA TTGGGTCATG GGAACATCTA ACCAAGCTGC AGATGAAGAT 
GTGAAATGTC TAACTAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTGGT GTCATTGCTC 
GCTGGGCTCC CTCTGCAGCC TGAGGAAGGC GATGGGGTGG AATTGATGGA AGCCAAATCC 
CAGCTGTTCC TTAAGTATTT CACCCTCTTT ATGAACCTTC TTAATGACTG CAGTGAGGTC 
GAAGAAGAAA ATGTCCAGAT TGGGGGCAGG AAGCGCGGCA TGTCGCGAAG GCTGGCGTCC 
CTGCGCCACT GCACCGTGCT GGCAATGTCC AACCTGCTCA ATGCGAATGT AGACAGCGGC 
CTGATGCACT CTATAGTGCT AGGATTGGGT TACCACAAAG ATCTTCAGAC AAGAGCCACC 
TTCATGGAAG TCCTCACAAA AATCCTTCAG CAAGGCACAG AGTTTGATAC GCTCGCAGAA 
ACCGTGTTAG CTGATCGATT TGAGAGACTT GTTGAGTTGG TCACGATGAT GGGTGATCAA 
GGAGAGCTCC CCATAGCCAT GGCTCTGGCC AACGTGGTGC CTTGTTCTCA GTGGGATGAA 
CTTGCTCGAG TCCTTGTTAC TCTCTTTGAT TCCCGACACT TACTGTATCA GCTCCTTTGG 
AACATGTTTT CTAAGGAAGT TGAGCTGGCA GACTCCATGC AGACACTTTT TAGAGGAAAC 
AGCTTGGCCA GTAAAATCAT GACCTTCTGC TTTAAGGTCT ACGGTGCTAC ATACCTGCAG 
AAGCTTCTGG AGCCTTTACT CCGCTTTGTG ATCACATCCT CCGAATGGCA GCACGTCAGC 
TTCGAGGTCG ATCCCACCAG GTTAGAGCAT ACTGAGAGCC TCGAGGACAA CCAGCGGAAC 
CTTTTGCAAA TGACTGAGAA GTTCTTCCAC GCCATCATCA GTTCTTCCTC CGAGTTCCCC 
CCTCAGCTAC GCAGTGTGTG CCACTGCCTG TACCAGGCAA CTTGCCACTC TCTACTGAAT 
AAAGCTACCG TAAAAGAAAA AAAGGAAAAC AAAAAATCAG TTGTTAGCCA GAGATTCCCT 
CAGAACAGCA TTGGCGCCGT GGGGAGTGCC ATGTTCCTGA GATTCATCAA CCCTGCCGTT 
GTCTCTCCCT ACGAGGCAGG GATTTTAGAG AGAAAACCGC TCCCTAGAAT TGAAAGGGGG 
TTGAAGCTGA TGTCAAAGAT TCTTCAGAGC ATTGCCAATC ATGTCCTTTT CACAAAAGAA 
GAGTATATGA GGCCTTTTAA CGATTTTGTG AAAAGCAACT TTGATGTAGC ACGGAGATTT 
TTCCTTGATA TCGCATCTGA TTGTCCTGTA AGTGATGCCA TAAACCACAG CCTTTCCTTC 
ATCAGCGATG GCAATGTCTT GGCTTTGCAC CGTCTGCTCT GGAACAACCA AGAGAAAATC 
GGCCAATATC TTTCAAGCAA CAGAGATCAC AAAGCTGTTG GAAGGCGACC TTTCGATAAG 
ATGGCAACAC TGCTTGCCTA CCTGGGACCT CCAGAGCACA AGCCTGTGGC TGACACACAC 
TGGTCCAGCC TAAATCTTAC CAGCTCCAAG TTTGAGGAAT TCATGACAAG GCACCAGGTA 
CATGAAAAAG AGGAATTCAA GGCTCTGAAA ACATTAAATA TCTTCTATCA AGCAGGCACT 
TCCAAAGCTG GAAATCCTGT TTTTTATTAT GTCACACGAA GGTTCAAGAC TGGCCAGATC 
AACGGGGATT TGCTTATATA CCACGTCTTA CTAACTTTAA AGCCATATTA TGCAAAGCCA 
TATGAGATTG TAATGGACCT CACTCATGCC GGGCCTAGCA ATCGCTTTAA GACAGACTTC 
CTCTCCAAGT GGTTTGTTGT TTTCCCGGGC TTTGCTTACG AGAACGTCTC TTCGGTCTAC 
ATCTACAACT GTAATTCTTG GGTTCGGGAG TACACCAAAT ACCATGAGAG ACTGCTCACG 
GGACTCAAAG GCAGCAAAAG ACTCTTTTTC ATAGACTCTC CCTCAAAACT GGCTGAGCAT 
ATCGATTATG ATCAGCAAAA ACTGCCAGCC GCGACCTTGG CTCTGGATGA AGACCTCAAG 
GTGTACAACA GTGCTCTCAA GCTGGCTCAC AAGGACACCA AGGTGACCCT CAAGGTTGGC 
TCTACTGCGA TCCAAGTGAC GTCATCTGAG CGAACTAAAG TCCTGGGACA GACGGTTTTT 
CTGAATGATA TTTACTATGC CTCTGAGATC GAAGAAATCT GTCTTGTGGA TGAAAACCAG 
TTCACTCTGA CCATTTCCAA TCAGGGCACT CCCCTCACCT TCATGCATCA AGAGTGTGAA 
GGCATTGTGG CGTCCATCAT TCACATCCGA ACTCGATGGG AGCTGTCCCA GCCAGATTCT 
ATCCCCCAGC ACACTAAGAT CCGGCCAAAG GACGTTCCCG GAACACTTTT GAATATCGCA 
TTACTTAACT TGGGTAGTTC GGACCCAAGT TTACGGTCAG CCGCCTATAA TCTTCTCTGT 
GCCTTAACCT GTACCTTTAA TTTAAAAATT GAGGGCCAAT TACTAGAAAC ATCAGGTCTG 
TGTATACCTG CCAACAATAC CCTTTTTATT GTCTCTATTA GTAAAACCCT GGCAGCTAAT 
GAGCCACACC TCACCTTAGA GTTTTTGGAA GAGTGCATTT CCGGATTTAG CAAATCCAGT 
ATTGAACTGA AGCACCTGTG TCTAGAATAC ATGACTCCCT GGCTATCAAA TCTAGTCCGT 
TTCTGTAAAC ATAATGATGA TGCCAAACGA CAGAGAGTCA CTGCTATTCT TGATAAACTT 
ATAACAATGA CCATAAATGA AAAGCAGATG TACCCATCGA TCCAAGCCAA GATATGGGGA 
AGCCTTGGGC AGATTACAGA TCTGCTCGAT GTAGTGCTGG ACAGCTTTAT CAAAACCAGT 
GCCACAGGTG GTTTAGGATC CATCAAGGCT GAAGTGATGG CTGACACGGC CGTGGCTCTG 
GCTTCTGGGA ACGTCAAACT AGTTTCGAGC AAGGTGATTG GAAGAATGTG CAAAATCATT 
GACAAGACCT GCCTCTCTCC AACTCCTACC TTAGAGCAGC ACCTCATGTG GGATGATATC 
GCCATTTTAG CACGCTACAT GCTAATGCTG TCCTTCAATA ATTCTCTGGA TGTGGCGGCT 
CATCTTCCCT ACCTCTTCCA TGTGGTGACT CTGTTGGTGG CCACAGGTCC CTTGTCCCTT 
CGTGCTTCCA CACACGGACT GGTCATCAAT ATCATCCATT CTCTGTGCAC GTGCTCACAG 
CTTCACTTTA GTGAAGAGAC CAAGCAAGTT TTACGACTGA GTCTGACTGA GTTCTCACTA 
CCTAAGTTTT ATTTGCTGTT CGGCATCAGC AAAGTCAAAT CAGCTGCCGT GATAGCCTTC 
CGGTCCAGTT ACCGGGACAG GTCTTTCTCC CCTGGCTCCT ATGAGAGGGA GACCTTTGCC 
CTGGCCTCCT TGGAAACAGT CACAGAAGCT CTCTTGGAGA TCATGGAGGC TTGTATGAGA 
GATATTCCTG CATGCAAGTG GCTGGACCAG TGGACAGAGC TAGCCCAGAG GTTTGCGTTC 
CAGTACAATC CCTCCCTGCA GCCACGAGCT CTGGTTGTGT TTGGTTGTAT CAGCAAAAGA 
GTGTCCCATG GGCAGATCAA GCAGATTATC CGCATTCTTA GCAAGGGACT TGAGAGCTGC 
CTAAAAGGAC CTGATAATTA TAACAGTCAA GTTCTGATTG AAGCTACCGT GATAGCTCTA 
ACCAAATTAC AACCACTTCT TAATAAGCAG GAGTCCCCTC TGCACCGAGC TCTCTTCTGG 
GTGGCCGTTG CCGTGCTGCA GCTGGATGAA GTGGACCTGT ACTCGGCGGG CACTGCCCTC 
CTCGAACAGA ACCTGCACAC CTTAGACAGC CTCCTGCACA TCTTCAACGA CCAGAGCCCA 
GAAGAAGTGT TCATGGAGAT CCGGCAGCCT CTGGAGTGGC ACTGCAAGCA GATGGACCAC 
TTTGTGGGGC TCAACTTTAA CTCCAGCTTT AACTTTGCCC TGGTCGGGCA CCTGCTGAAG 
GGATTTCGGC ATCCTTCACC TGTCACTGTT GCAAGAACTG TGAGGATTTT GCACACCCTA 
CTAGCATTGG TTAACAAGCA TAGGAATTGT GACAAGTTTG AGGTGAACAC CCAGAGTGTG 
GCTTACTTAG CAGCTTTACT TACTGTGTCT GAGGAGGTTC GAAGTCGCTG CAGCTTAAAG 
CATAGGAAAT CACTCCTACT AACTGATATC ACAATGGAAA ATGTGCCTAT GGATATATAT 
CCCATCTATC ACAGTGACCC CAGCCTTAGG ACACTAAAAG AAACACAACC GTGGTCCTCT 
CCAAAGGGAT CTGAGAGACA TCTTGCAGCC AGCTACCCTC CTGTGGGCCA AACTAGCCCC 
CGAACCAGAA AATCCATGAG CCTGGATATG GGGCAGCCTT CCCAGGCCAA CACAAAGAAG 
TTGCTTGGGA CAAGGAAAAG CTTCGATCAT TTGATCTCTG ATGCCAAAGC TCCCAAAAGG 
CAAGAACAGG AGTCTGGAGT CACCACGCCC CCCAAGATGC GGAGAGTGGC CGAGAACGAC 
TACGAGATGG AAACCCAGAG AATCTCCCCT CAGCCACACC CGCCTCTGCG GAAAGTGTCC 
GTGTCCGAAT CCAATGTGCT GCTGGACGAG GAAGTGCTCA CAGACCCCAA AACCCAGGCT 
CTTCTTCTCA CTGTCCTGGC TACTCTGGTG AAGTACACTG CGGACGAGTT TGACCAGCGC 
ATTCTCTATG AGTACTTAGC GGAGGCCAGT GTTGTCTTCC CAAAGGTTTT TCCAGTGGTA 
CATAATTTGT TGGACTCAAA GATAAATACT CTGCTCTCAT TATGCCAAGA CCCAAATTTG 
CTAAACCCGG TTCATGGAAT TGTGCAGAGT GTGGTCTACC ACGAAGAGGC CCCGCCGCAG 
TACCAGACGG CGTACCTCCA GAGTTTCGGT TTTAATGGTC TGTGGAGGTT TGCAGGACCA 
TTTTCTAAGC AGACGCAGAT CCCAGACTAT GCCGAGCTCA TCGTGAAGTT TCTCGATGCC 
TTGATTGACA CGTATCTGCC TGGAATTGAT GAGGAAGCCA GTGAGGAGTC CCTCCTGACA 
CCCACCTCTC CCTACCCGCC CGCGGTGCAG AGCCAGCTTA GCATCACTGC GAACCTCAAC 
CTCTCTAACT CCATGACCTC ACTGGCAACT TCCCAGCATT CCCCAGGCAT CGACAAGGAG 
AATGTGGAGC TCTCGCCAAC CACCGGGCAC TGCAGCGGCG GGAGGACGCG CCACGGCTCC 
GCCAGCCAGG TCCAGAAGCA AAGGAGTGCC GGCAGCTTCA AGAGGCACAG CATCAAGAAG 
ATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823505.1
CDS	9..8657
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKFSTLLTTITAWILSPWLCDTDVSIPLSCQITRPQSSFKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIVLGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKQESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967645.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967645.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGCAGCCA AAGGACACGA TGAGGTTAGA TGAAACAATG 
CTGGTCAAAC AGTTGCTGCC GGAAATCTGC CATTTCCTTC ACACCTACCG GGAAGGGAAC 
CAGCACGCAG CCGAGCTTCG GAGCTCCGCC TCTGGCGTCC TTTTCTCCCT CAGCTGTAAT 
AACTTCAATG CCGTCTTCAG CCGGATTTCC ACCAGGTTAC AGGAATTGAC TGTTTGCTCA 
GAAGATAATG TTGATGTCCA TGATATAGAA TTATTACAAT ATATCAATGT GGATTGTGCA 
AAACTAAAGC GGCTCTTGCA GGAAACTGTA TTTAAGTTTA AAGCCTTAAA GAAGGTAGCT 
CAACTAGCAG TTATAAATAG CCTAGAAAAG GCATTTTGGA ATTGGGTAGA GAACTATCCT 
GATGAATTTA CAAAGCTGTA CCAGACTCCC CAGACAGATA TGGCGGAATG TGCAGAAAAA 
TTATTTGACT TGGTGGATGG TTTTGCTGAA AGCACCAAAC GTAAAGCTGC AGTTTGGCCA 
CTGCAAATCA TTCTTCTTGT CTTGTGTCCA GAAATAATCC AGGACATTTC CAAGGATGTG 
GTTGATGAAG CGAAAATGAA TAAGTTTTCT ACACTGTTGA CCACTATCAC CGCCTGGATA 
CTTTCTCCTT GGCTTTGTGA TACCGATGTC TCAATTCCTC TTTCCTGCCA AATCACTCGT 
CCTCAGTCCT CCTTTAAAAT GTTTCTCGAC AATCTCAGAA GAGCGCTGGC CGGCCACAGT 
GGGAGCAGGC AGCTGACAGA GAGTGCCGCG ATCGCTTGTG TTAAACTGTG CAAAGCCAGC 
ACCTACATCA ACTGGGAGGA TAACTCGGTC ATTTTCCTGC TCGTCCAATC CATGGTGGTG 
GATCTGAAGA ACTTGCTCTT TAATCCCAGT AAGCCCTTCT CCAGAGGCAG CCAGAATGCT 
GATGTGGACC TCATGATAGA CTGCCTGGTT TCTTGCTTTC GCATCAGCCC TCACAACAAC 
CAACACTTTA AGATCTGTTT GGCCCAGAAC TCCCCATCTA CATTTCATTA TGTGCTGGTA 
AACTCCCTCC ATCGAATTAT CACCAATAGC ACTTTCAAGC ATGGACTTGG CACTGCTTCC 
GCCCTGGATT GGTGGCCCAA GATTGATGCT GTGTATTGCC ATTCTGTAGA ACTTCGAAAC 
ATGTTTGGTG AAACGCTTCA CAAGGCCGTG CAGGGCTGTG GGCCTCACCC CGCGATACGA 
ATGGCGCCGA GTCTTACATT TAAAGAAAAA ATGACAAGCC TTAAATTTAA AGAAAAACCT 
ACGGACTTGG AAACAAGAAG TTATAAATAC CTCTTATTAT CCATGGTGAA ACTAATCCAT 
GCAGATCCAA AGCTGTTGCT ATGTAATCCA AGAAAACAAG GGCCTGAAAC TCAGGGGAGT 
ACAGCAGAGT TAATCACAGG CCTTGTGCAG CTCGTCCCAC AGTCCAGCAT GCCAGAAATA 
GCTCAAGAAG CAATGGAGGC TTTGCTGGTT CTTCATCAGT TGGATAGTAT TGACTTATGG 
AATCCAGATT CACCTATAGA AACATTCTGG GAGATTAGCT CACAAATGCT TTTTTATATC 
TGTAAAAAGC TAACTACCCA TCAGATGCTT AGCAGTACAG AAATTCTCAA GTGGTTGCGA 
GAAATTTTGA TCTGTAGGAA CAAATATCTT CTTAAAAATA AGCAGTCAGA TAGGAGTTCT 
TCCCATTTCC TCTACCTTTA TGGGGCAGGC TATGATGTCT CTTCTGGTGG AGGTACCAGT 
CATATCTCTC TAGACCACGA GGAGTTTATG CGTACCTGTG CTCATGGACC ATCTCTCAGG 
AAAGTGAAAG GAAGCTCCTC AATGGACGGT GCAATCGGGT CCAGCGGGAC GCCCATCTGC 
CGCCAGGCCC AGACTAAGCT GGAGGTTGCT CTGTACATGT TTCTGTGGAG TCCCGACATT 
GAAGCCGTGC TCGTGGCCAT GTCCTGCTTT CGACACCTGT GTGAGGAAGC CGACATCAGG 
TGTGGCATAG ATGAGATCTC GGTGCACCAT TTCTTGCCAA ACTATAACAC CTTCATGGAG 
TTCGCATCTG TCAGCAACAT GATGTCGACA GGAAGAGCAG CTCTTCAGAA AAGAGTGATG 
GCCCTGCTCA GGCGCATCGA ACACCCCACC GCTGGAAACA CCGAGGCTTG GGAAGATACT 
TACGCCAAAT GGGAGCAAGC TACAAAATCA ATCCTCAATT ATCCGAAAGC CAAAATTGAA 
GATGGCCAGG TTGCTGAGAG TCTTCACAAG ACCATTGTGA AGAGAAGAAT GTCTCACGTG 
AGTGGAGGGG GCTCCATAGA CTTGTGCGAC ACTGACTCGC TACAGGAGTG GATCAACATG 
ACTGGGTTTC TCTGTGCCCT TGGTGGCGTG TGCCTACAGC AGCGGAGCAA TGCCGGCTTT 
GCCACCTACA GCCCTCCCAT GGGCCCTATC AGTGAACGCA AGGGCTCTCT CATTTCCATG 
GTGTCCAGCG ATGGAAACGC AGAGACGCCT GTCAGCAAGT TCATGGATCG GCTGCTCTCC 
TTGATGGTGT GTAACCACGA AAAAGTGGGG CTGCAGATAC GAAGCAATGT CAAGGACTTG 
TTGGGTCTTG AATTGAGTCC TGCCCTTTAT CCAATGCTGT TTAACAAATT AAAAAATACC 
ATCAGCAAGT TTTTTGATCC TCAAGGACAG GTTTTATTGA CTGACACCAA CACCCAGTTT 
GTGGAGCAGA CCATAGCCAT AATGAAGAAC TTGCTTGATA ATCATGCTGA GGGTAGTTCT 
GAACACATGG GACAAGCTAG CATCGAAACT ATGATGTTAA ATCTGGTCAG ATATGTTAGA 
GTGTTTGGGA ATTTGGTGCA GGCAATTCAG ATCAAAACTA AGCTGTGCCA GCTGGTTCAA 
GTAATGATGG AGAGGAGAGA TGACCTTTCA TTTTGCCAAG AGATGAAATT TAGGAATAAA 
ATGGTAGAAT ATCTGACAGA TTGGGTCATG GGAACATCTA ACCAAGCTGC AGATGAAGAT 
GTGAAATGTC TAACTAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTGGT GTCATTGCTC 
GCTGGGCTCC CTCTGCAGCC TGAGGAAGGC GATGGGGTGG AATTGATGGA AGCCAAATCC 
CAGCTGTTCC TTAAGTATTT CACCCTCTTT ATGAACCTTC TTAATGACTG CAGTGAGGTC 
GAAGAAGAAA ATGTCCAGAT TGGGGGCAGG AAGCGCGGCA TGTCGCGAAG GCTGGCGTCC 
CTGCGCCACT GCACCGTGCT GGCAATGTCC AACCTGCTCA ATGCGAATGT AGACAGCGGC 
CTGATGCACT CTATAGTGCT AGGATTGGGT TACCACAAAG ATCTTCAGAC AAGAGCCACC 
TTCATGGAAG TCCTCACAAA AATCCTTCAG CAAGGCACAG AGTTTGATAC GCTCGCAGAA 
ACCGTGTTAG CTGATCGATT TGAGAGACTT GTTGAGTTGG TCACGATGAT GGGTGATCAA 
GGAGAGCTCC CCATAGCCAT GGCTCTGGCC AACGTGGTGC CTTGTTCTCA GTGGGATGAA 
CTTGCTCGAG TCCTTGTTAC TCTCTTTGAT TCCCGACACT TACTGTATCA GCTCCTTTGG 
AACATGTTTT CTAAGGAAGT TGAGCTGGCA GACTCCATGC AGACACTTTT TAGAGGAAAC 
AGCTTGGCCA GTAAAATCAT GACCTTCTGC TTTAAGGTCT ACGGTGCTAC ATACCTGCAG 
AAGCTTCTGG AGCCTTTACT CCGCTTTGTG ATCACATCCT CCGAATGGCA GCACGTCAGC 
TTCGAGGTCG ATCCCACCAG GTTAGAGCAT ACTGAGAGCC TCGAGGACAA CCAGCGGAAC 
CTTTTGCAAA TGACTGAGAA GTTCTTCCAC GCCATCATCA GTTCTTCCTC CGAGTTCCCC 
CCTCAGCTAC GCAGTGTGTG CCACTGCCTG TACCAGGCAA CTTGCCACTC TCTACTGAAT 
AAAGCTACCG TAAAAGAAAA AAAGGAAAAC AAAAAATCAG TTGTTAGCCA GAGATTCCCT 
CAGAACAGCA TTGGCGCCGT GGGGAGTGCC ATGTTCCTGA GATTCATCAA CCCTGCCGTT 
GTCTCTCCCT ACGAGGCAGG GATTTTAGAG AGAAAACCGC TCCCTAGAAT TGAAAGGGGG 
TTGAAGCTGA TGTCAAAGAT TCTTCAGAGC ATTGCCAATC ATGTCCTTTT CACAAAAGAA 
GAGTATATGA GGCCTTTTAA CGATTTTGTG AAAAGCAACT TTGATGTAGC ACGGAGATTT 
TTCCTTGATA TCGCATCTGA TTGTCCTGTA AGTGATGCCA TAAACCACAG CCTTTCCTTC 
ATCAGCGATG GCAATGTCTT GGCTTTGCAC CGTCTGCTCT GGAACAACCA AGAGAAAATC 
GGCCAATATC TTTCAAGCAA CAGAGATCAC AAAGCTGTTG GAAGGCGACC TTTCGATAAG 
ATGGCAACAC TGCTTGCCTA CCTGGGACCT CCAGAGCACA AGCCTGTGGC TGACACACAC 
TGGTCCAGCC TAAATCTTAC CAGCTCCAAG TTTGAGGAAT TCATGACAAG GCACCAGGTA 
CATGAAAAAG AGGAATTCAA GGCTCTGAAA ACATTAAATA TCTTCTATCA AGCAGGCACT 
TCCAAAGCTG GAAATCCTGT TTTTTATTAT GTCACACGAA GGTTCAAGAC TGGCCAGATC 
AACGGGGATT TGCTTATATA CCACGTCTTA CTAACTTTAA AGCCATATTA TGCAAAGCCA 
TATGAGATTG TAATGGACCT CACTCATGCC GGGCCTAGCA ATCGCTTTAA GACAGACTTC 
CTCTCCAAGT GGTTTGTTGT TTTCCCGGGC TTTGCTTACG AGAACGTCTC TTCGGTCTAC 
ATCTACAACT GTAATTCTTG GGTTCGGGAG TACACCAAAT ACCATGAGAG ACTGCTCACG 
GGACTCAAAG GCAGCAAAAG ACTCTTTTTC ATAGACTCTC CCTCAAAACT GGCTGAGCAT 
ATCGATTATG ATCAGCAAAA ACTGCCAGCC GCGACCTTGG CTCTGGATGA AGACCTCAAG 
GTGTACAACA GTGCTCTCAA GCTGGCTCAC AAGGACACCA AGGTGACCCT CAAGGTTGGC 
TCTACTGCGA TCCAAGTGAC GTCATCTGAG CGAACTAAAG TCCTGGGACA GACGGTTTTT 
CTGAATGATA TTTACTATGC CTCTGAGATC GAAGAAATCT GTCTTGTGGA TGAAAACCAG 
TTCACTCTGA CCATTTCCAA TCAGGGCACT CCCCTCACCT TCATGCATCA AGAGTGTGAA 
GGCATTGTGG CGTCCATCAT TCACATCCGA ACTCGATGGG AGCTGTCCCA GCCAGATTCT 
ATCCCCCAGC ACACTAAGAT CCGGCCAAAG GACGTTCCCG GAACACTTTT GAATATCGCA 
TTACTTAACT TGGGTAGTTC GGACCCAAGT TTACGGTCAG CCGCCTATAA TCTTCTCTGT 
GCCTTAACCT GTACCTTTAA TTTAAAAATT GAGGGCCAAT TACTAGAAAC ATCAGGTCTG 
TGTATACCTG CCAACAATAC CCTTTTTATT GTCTCTATTA GTAAAACCCT GGCAGCTAAT 
GAGCCACACC TCACCTTAGA GTTTTTGGAA GAGTGCATTT CCGGATTTAG CAAATCCAGT 
ATTGAACTGA AGCACCTGTG TCTAGAATAC ATGACTCCCT GGCTATCAAA TCTAGTCCGT 
TTCTGTAAAC ATAATGATGA TGCCAAACGA CAGAGAGTCA CTGCTATTCT TGATAAACTT 
ATAACAATGA CCATAAATGA AAAGCAGATG TACCCATCGA TCCAAGCCAA GATATGGGGA 
AGCCTTGGGC AGATTACAGA TCTGCTCGAT GTAGTGCTGG ACAGCTTTAT CAAAACCAGT 
GCCACAGGTG GTTTAGGATC CATCAAGGCT GAAGTGATGG CTGACACGGC CGTGGCTCTG 
GCTTCTGGGA ACGTCAAACT AGTTTCGAGC AAGGTGATTG GAAGAATGTG CAAAATCATT 
GACAAGACCT GCCTCTCTCC AACTCCTACC TTAGAGCAGC ACCTCATGTG GGATGATATC 
GCCATTTTAG CACGCTACAT GCTAATGCTG TCCTTCAATA ATTCTCTGGA TGTGGCGGCT 
CATCTTCCCT ACCTCTTCCA TGTGGTGACT CTGTTGGTGG CCACAGGTCC CTTGTCCCTT 
CGTGCTTCCA CACACGGACT GGTCATCAAT ATCATCCATT CTCTGTGCAC GTGCTCACAG 
CTTCACTTTA GTGAAGAGAC CAAGCAAGTT TTACGACTGA GTCTGACTGA GTTCTCACTA 
CCTAAGTTTT ATTTGCTGTT CGGCATCAGC AAAGTCAAAT CAGCTGCCGT GATAGCCTTC 
CGGTCCAGTT ACCGGGACAG GTCTTTCTCC CCTGGCTCCT ATGAGAGGGA GACCTTTGCC 
CTGGCCTCCT TGGAAACAGT CACAGAAGCT CTCTTGGAGA TCATGGAGGC TTGTATGAGA 
GATATTCCTG CATGCAAGTG GCTGGACCAG TGGACAGAGC TAGCCCAGAG GTTTGCGTTC 
CAGTACAATC CCTCCCTGCA GCCACGAGCT CTGGTTGTGT TTGGTTGTAT CAGCAAAAGA 
GTGTCCCATG GGCAGATCAA GCAGATTATC CGCATTCTTA GCAAGGGACT TGAGAGCTGC 
CTAAAAGGAC CTGATAATTA TAACAGTCAA GTTCTGATTG AAGCTACCGT GATAGCTCTA 
ACCAAATTAC AACCACTTCT TAATAAGCAG GAGTCCCCTC TGCACCGAGC TCTCTTCTGG 
GTGGCCGTTG CCGTGCTGCA GCTGGATGAA GTGGACCTGT ACTCGGCGGG CACTGCCCTC 
CTCGAACAGA ACCTGCACAC CTTAGACAGC CTCCTGCACA TCTTCAACGA CCAGAGCCCA 
GAAGAAGTGT TCATGGAGAT CCGGCAGCCT CTGGAGTGGC ACTGCAAGCA GATGGACCAC 
TTTGTGGGGC TCAACTTTAA CTCCAGCTTT AACTTTGCCC TGGTCGGGCA CCTGCTGAAG 
GGATTTCGGC ATCCTTCACC TGTCACTGTT GCAAGAACTG TGAGGATTTT GCACACCCTA 
CTAGCATTGG TTAACAAGCA TAGGAATTGT GACAAGTTTG AGGTGAACAC CCAGAGTGTG 
GCTTACTTAG CAGCTTTACT TACTGTGTCT GAGGAGGTTC GAAGTCGCTG CAGCTTAAAG 
CATAGGAAAT CACTCCTACT AACTGATATC ACAATGGAAA ATGTGCCTAT GGATATATAT 
CCCATCTATC ACAGTGACCC CAGCCTTAGG ACACTAAAAG AAACACAACC GTGGTCCTCT 
CCAAAGGGAT CTGAGAGACA TCTTGCAGCC AGCTACCCTC CTGTGGGCCA AACTAGCCCC 
CGAACCAGAA AATCCATGAG CCTGGATATG GGGCAGCCTT CCCAGGCCAA CACAAAGAAG 
TTGCTTGGGA CAAGGAAAAG CTTCGATCAT TTGATCTCTG ATGCCAAAGC TCCCAAAAGG 
CAAGAACAGG AGTCTGGAGT CACCACGCCC CCCAAGATGC GGAGAGTGGC CGAGAACGAC 
TACGAGATGG AAACCCAGAG AATCTCCCCT CAGCCACACC CGCCTCTGCG GAAAGTGTCC 
GTGTCCGAAT CCAATGTGCT GCTGGACGAG GAAGTGCTCA CAGACCCCAA AACCCAGGCT 
CTTCTTCTCA CTGTCCTGGC TACTCTGGTG AAGTACACTG CGGACGAGTT TGACCAGCGC 
ATTCTCTATG AGTACTTAGC GGAGGCCAGT GTTGTCTTCC CAAAGGTTTT TCCAGTGGTA 
CATAATTTGT TGGACTCAAA GATAAATACT CTGCTCTCAT TATGCCAAGA CCCAAATTTG 
CTAAACCCGG TTCATGGAAT TGTGCAGAGT GTGGTCTACC ACGAAGAGGC CCCGCCGCAG 
TACCAGACGG CGTACCTCCA GAGTTTCGGT TTTAATGGTC TGTGGAGGTT TGCAGGACCA 
TTTTCTAAGC AGACGCAGAT CCCAGACTAT GCCGAGCTCA TCGTGAAGTT TCTCGATGCC 
TTGATTGACA CGTATCTGCC TGGAATTGAT GAGGAAGCCA GTGAGGAGTC CCTCCTGACA 
CCCACCTCTC CCTACCCGCC CGCGGTGCAG AGCCAGCTTA GCATCACTGC GAACCTCAAC 
CTCTCTAACT CCATGACCTC ACTGGCAACT TCCCAGCATT CCCCAGGCAT CGACAAGGAG 
AATGTGGAGC TCTCGCCAAC CACCGGGCAC TGCAGCGGCG GGAGGACGCG CCACGGCTCC 
GCCAGCCAGG TCCAGAAGCA AAGGAGTGCC GGCAGCTTCA AGAGGCACAG CATCAAGAAG 
ATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967647.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823507.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X8, mRNA.

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CloneID	OSa234695
Clone ID Related Accession (Same CDS sequence)	XM_031967647.1
Accession Version	XM_031967647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGCCAAAG GACACGATGA GGTTAGATGA AACAATGCTG 
GTCAAACAGT TGCTGCCGGA AATCTGCCAT TTCCTTCACA CCTACCGGGA AGGGAACCAG 
CACGCAGCCG AGCTTCGGAG CTCCGCCTCT GGCGTCCTTT TCTCCCTCAG CTGTAATAAC 
TTCAATGCCG TCTTCAGCCG GATTTCCACC AGGTTACAGG AATTGACTGT TTGCTCAGAA 
GATAATGTTG ATGTCCATGA TATAGAATTA TTACAATATA TCAATGTGGA TTGTGCAAAA 
CTAAAGCGGC TCTTGCAGGA AACTGTATTT AAGTTTAAAG CCTTAAAGAA GGTAGCTCAA 
CTAGCAGTTA TAAATAGCCT AGAAAAGGCA TTTTGGAATT GGGTAGAGAA CTATCCTGAT 
GAATTTACAA AGCTGTACCA GACTCCCCAG ACAGATATGG CGGAATGTGC AGAAAAATTA 
TTTGACTTGG TGGATGGTTT TGCTGAAAGC ACCAAACGTA AAGCTGCAGT TTGGCCACTG 
CAAATCATTC TTCTTGTCTT GTGTCCAGAA ATAATCCAGG ACATTTCCAA GGATGTGGTT 
GATGAAGCGA AAATGAATAA GTTTTCTACA CTGTTGACCA CTATCACCGC CTGGATACTT 
TCTCCTTGGC TTTGTGATAC CGATGTCTCA ATTCCTCTTT CCTGCCAAAT CACTCGTCCT 
CAGTCCTCCT TTAAAATGTT TCTCGACAAT CTCAGAAGAG CGCTGGCCGG CCACAGTGGG 
AGCAGGCAGC TGACAGAGAG TGCCGCGATC GCTTGTGTTA AACTGTGCAA AGCCAGCACC 
TACATCAACT GGGAGGATAA CTCGGTCATT TTCCTGCTCG TCCAATCCAT GGTGGTGGAT 
CTGAAGAACT TGCTCTTTAA TCCCAGTAAG CCCTTCTCCA GAGGCAGCCA GAATGCTGAT 
GTGGACCTCA TGATAGACTG CCTGGTTTCT TGCTTTCGCA TCAGCCCTCA CAACAACCAA 
CACTTTAAGA TCTGTTTGGC CCAGAACTCC CCATCTACAT TTCATTATGT GCTGGTAAAC 
TCCCTCCATC GAATTATCAC CAATAGCACT TTCAAGCATG GACTTGGCAC TGCTTCCGCC 
CTGGATTGGT GGCCCAAGAT TGATGCTGTG TATTGCCATT CTGTAGAACT TCGAAACATG 
TTTGGTGAAA CGCTTCACAA GGCCGTGCAG GGCTGTGGGC CTCACCCCGC GATACGAATG 
GCGCCGAGTC TTACATTTAA AGAAAAAATG ACAAGCCTTA AATTTAAAGA AAAACCTACG 
GACTTGGAAA CAAGAAGTTA TAAATACCTC TTATTATCCA TGGTGAAACT AATCCATGCA 
GATCCAAAGC TGTTGCTATG TAATCCAAGA AAACAAGGGC CTGAAACTCA GGGGAGTACA 
GCAGAGTTAA TCACAGGCCT TGTGCAGCTC GTCCCACAGT CCAGCATGCC AGAAATAGCT 
CAAGAAGCAA TGGAGGCTTT GCTGGTTCTT CATCAGTTGG ATAGTATTGA CTTATGGAAT 
CCAGATTCAC CTATAGAAAC ATTCTGGGAG ATTAGCTCAC AAATGCTTTT TTATATCTGT 
AAAAAGCTAA CTACCCATCA GATGCTTAGC AGTACAGAAA TTCTCAAGTG GTTGCGAGAA 
ATTTTGATCT GTAGGAACAA ATATCTTCTT AAAAATAAGC AGTCAGATAG GAGTTCTTCC 
CATTTCCTCT ACCTTTATGG GGCAGGCTAT GATGTCTCTT CTGGTGGAGG TACCAGTCAT 
ATCTCTCTAG ACCACGAGGA GTTTATGCGT ACCTGTGCTC ATGGACCATC TCTCAGGAAA 
GTGAAAGGAA GCTCCTCAAT GGACGGTGCA ATCGGGTCCA GCGGGACGCC CATCTGCCGC 
CAGGCCCAGA CTAAGCTGGA GGTTGCTCTG TACATGTTTC TGTGGAGTCC CGACATTGAA 
GCCGTGCTCG TGGCCATGTC CTGCTTTCGA CACCTGTGTG AGGAAGCCGA CATCAGGTGT 
GGCATAGATG AGATCTCGGT GCACCATTTC TTGCCAAACT ATAACACCTT CATGGAGTTC 
GCATCTGTCA GCAACATGAT GTCGACAGGA AGAGCAGCTC TTCAGAAAAG AGTGATGGCC 
CTGCTCAGGC GCATCGAACA CCCCACCGCT GGAAACACCG AGGCTTGGGA AGATACTTAC 
GCCAAATGGG AGCAAGCTAC AAAATCAATC CTCAATTATC CGAAAGCCAA AATTGAAGAT 
GGCCAGGTTG CTGAGAGTCT TCACAAGACC ATTGTGAAGA GAAGAATGTC TCACGTGAGT 
GGAGGGGGCT CCATAGACTT GTGCGACACT GACTCGCTAC AGGAGTGGAT CAACATGACT 
GGGTTTCTCT GTGCCCTTGG TGGCGTGTGC CTACAGCAGC GGAGCAATGC CGGCTTTGCC 
ACCTACAGCC CTCCCATGGG CCCTATCAGT GAACGCAAGG GCTCTCTCAT TTCCATGGTG 
TCCAGCGATG GAAACGCAGA GACGCCTGTC AGCAAGTTCA TGGATCGGCT GCTCTCCTTG 
ATGGTGTGTA ACCACGAAAA AGTGGGGCTG CAGATACGAA GCAATGTCAA GGACTTGTTG 
GGTCTTGAAT TGAGTCCTGC CCTTTATCCA ATGCTGTTTA ACAAATTAAA AAATACCATC 
AGCAAGTTTT TTGATCCTCA AGGACAGGTT TTATTGACTG ACACCAACAC CCAGTTTGTG 
GAGCAGACCA TAGCCATAAT GAAGAACTTG CTTGATAATC ATGCTGAGGG TAGTTCTGAA 
CACATGGGAC AAGCTAGCAT CGAAACTATG ATGTTAAATC TGGTCAGATA TGTTAGAGTG 
TTTGGGAATT TGGTGCAGGC AATTCAGATC AAAACTAAGC TGTGCCAGCT GGTTCAAGTA 
ATGATGGAGA GGAGAGATGA CCTTTCATTT TGCCAAGAGA TGAAATTTAG GAATAAAATG 
GTAGAATATC TGACAGATTG GGTCATGGGA ACATCTAACC AAGCTGCAGA TGAAGATGTG 
AAATGTCTAA CTAGAGATTT GGACCAGGCA AGCATGGAAG CAGTGGTGTC ATTGCTCGCT 
GGGCTCCCTC TGCAGCCTGA GGAAGGCGAT GGGGTGGAAT TGATGGAAGC CAAATCCCAG 
CTGTTCCTTA AGTATTTCAC CCTCTTTATG AACCTTCTTA ATGACTGCAG TGAGGTCGAA 
GAAGAAAATG TCCAGATTGG GGGCAGGAAG CGCGGCATGT CGCGAAGGCT GGCGTCCCTG 
CGCCACTGCA CCGTGCTGGC AATGTCCAAC CTGCTCAATG CGAATGTAGA CAGCGGCCTG 
ATGCACTCTA TAGTGCTAGG ATTGGGTTAC CACAAAGATC TTCAGACAAG AGCCACCTTC 
ATGGAAGTCC TCACAAAAAT CCTTCAGCAA GGCACAGAGT TTGATACGCT CGCAGAAACC 
GTGTTAGCTG ATCGATTTGA GAGACTTGTT GAGTTGGTCA CGATGATGGG TGATCAAGGA 
GAGCTCCCCA TAGCCATGGC TCTGGCCAAC GTGGTGCCTT GTTCTCAGTG GGATGAACTT 
GCTCGAGTCC TTGTTACTCT CTTTGATTCC CGACACTTAC TGTATCAGCT CCTTTGGAAC 
ATGTTTTCTA AGGAAGTTGA GCTGGCAGAC TCCATGCAGA CACTTTTTAG AGGAAACAGC 
TTGGCCAGTA AAATCATGAC CTTCTGCTTT AAGGTCTACG GTGCTACATA CCTGCAGAAG 
CTTCTGGAGC CTTTACTCCG CTTTGTGATC ACATCCTCCG AATGGCAGCA CGTCAGCTTC 
GAGGTCGATC CCACCAGGTT AGAGCATACT GAGAGCCTCG AGGACAACCA GCGGAACCTT 
TTGCAAATGA CTGAGAAGTT CTTCCACGCC ATCATCAGTT CTTCCTCCGA GTTCCCCCCT 
CAGCTACGCA GTGTGTGCCA CTGCCTGTAC CAGGCAACTT GCCACTCTCT ACTGAATAAA 
GCTACCGTAA AAGAAAAAAA GGAAAACAAA AAATCAGTTG TTAGCCAGAG ATTCCCTCAG 
AACAGCATTG GCGCCGTGGG GAGTGCCATG TTCCTGAGAT TCATCAACCC TGCCGTTGTC 
TCTCCCTACG AGGCAGGGAT TTTAGAGAGA AAACCGCTCC CTAGAATTGA AAGGGGGTTG 
AAGCTGATGT CAAAGATTCT TCAGAGCATT GCCAATCATG TCCTTTTCAC AAAAGAAGAG 
TATATGAGGC CTTTTAACGA TTTTGTGAAA AGCAACTTTG ATGTAGCACG GAGATTTTTC 
CTTGATATCG CATCTGATTG TCCTGTAAGT GATGCCATAA ACCACAGCCT TTCCTTCATC 
AGCGATGGCA ATGTCTTGGC TTTGCACCGT CTGCTCTGGA ACAACCAAGA GAAAATCGGC 
CAATATCTTT CAAGCAACAG AGATCACAAA GCTGTTGGAA GGCGACCTTT CGATAAGATG 
GCAACACTGC TTGCCTACCT GGGACCTCCA GAGCACAAGC CTGTGGCTGA CACACACTGG 
TCCAGCCTAA ATCTTACCAG CTCCAAGTTT GAGGAATTCA TGACAAGGCA CCAGGTACAT 
GAAAAAGAGG AATTCAAGGC TCTGAAAACA TTAAATATCT TCTATCAAGC AGGCACTTCC 
AAAGCTGGAA ATCCTGTTTT TTATTATGTC ACACGAAGGT TCAAGACTGG CCAGATCAAC 
GGGGATTTGC TTATATACCA CGTCTTACTA ACTTTAAAGC CATATTATGC AAAGCCATAT 
GAGATTGTAA TGGACCTCAC TCATGCCGGG CCTAGCAATC GCTTTAAGAC AGACTTCCTC 
TCCAAGTGGT TTGTTGTTTT CCCGGGCTTT GCTTACGAGA ACGTCTCTTC GGTCTACATC 
TACAACTGTA ATTCTTGGGT TCGGGAGTAC ACCAAATACC ATGAGAGACT GCTCACGGGA 
CTCAAAGGCA GCAAAAGACT CTTTTTCATA GACTCTCCCT CAAAACTGGC TGAGCATATC 
GATTATGATC AGCAAAAACT GCCAGCCGCG ACCTTGGCTC TGGATGAAGA CCTCAAGGTG 
TACAACAGTG CTCTCAAGCT GGCTCACAAG GACACCAAGG TGACCCTCAA GGTTGGCTCT 
ACTGCGATCC AAGTGACGTC ATCTGAGCGA ACTAAAGTCC TGGGACAGAC GGTTTTTCTG 
AATGATATTT ACTATGCCTC TGAGATCGAA GAAATCTGTC TTGTGGATGA AAACCAGTTC 
ACTCTGACCA TTTCCAATCA GGGCACTCCC CTCACCTTCA TGCATCAAGA GTGTGAAGGC 
ATTGTGGCGT CCATCATTCA CATCCGAACT CGATGGGAGC TGTCCCAGCC AGATTCTATC 
CCCCAGCACA CTAAGATCCG GCCAAAGGAC GTTCCCGGAA CACTTTTGAA TATCGCATTA 
CTTAACTTGG GTAGTTCGGA CCCAAGTTTA CGGTCAGCCG CCTATAATCT TCTCTGTGCC 
TTAACCTGTA CCTTTAATTT AAAAATTGAG GGCCAATTAC TAGAAACATC AGGTCTGTGT 
ATACCTGCCA ACAATACCCT TTTTATTGTC TCTATTAGTA AAACCCTGGC AGCTAATGAG 
CCACACCTCA CCTTAGAGTT TTTGGAAGAG TGCATTTCCG GATTTAGCAA ATCCAGTATT 
GAACTGAAGC ACCTGTGTCT AGAATACATG ACTCCCTGGC TATCAAATCT AGTCCGTTTC 
TGTAAACATA ATGATGATGC CAAACGACAG AGAGTCACTG CTATTCTTGA TAAACTTATA 
ACAATGACCA TAAATGAAAA GCAGATGTAC CCATCGATCC AAGCCAAGAT ATGGGGAAGC 
CTTGGGCAGA TTACAGATCT GCTCGATGTA GTGCTGGACA GCTTTATCAA AACCAGTGCC 
ACAGGTGGTT TAGGATCCAT CAAGGCTGAA GTGATGGCTG ACACGGCCGT GGCTCTGGCT 
TCTGGGAACG TCAAACTAGT TTCGAGCAAG GTGATTGGAA GAATGTGCAA AATCATTGAC 
AAGACCTGCC TCTCTCCAAC TCCTACCTTA GAGCAGCACC TCATGTGGGA TGATATCGCC 
ATTTTAGCAC GCTACATGCT AATGCTGTCC TTCAATAATT CTCTGGATGT GGCGGCTCAT 
CTTCCCTACC TCTTCCATGT GGTGACTCTG TTGGTGGCCA CAGGTCCCTT GTCCCTTCGT 
GCTTCCACAC ACGGACTGGT CATCAATATC ATCCATTCTC TGTGCACGTG CTCACAGCTT 
CACTTTAGTG AAGAGACCAA GCAAGTTTTA CGACTGAGTC TGACTGAGTT CTCACTACCT 
AAGTTTTATT TGCTGTTCGG CATCAGCAAA GTCAAATCAG CTGCCGTGAT AGCCTTCCGG 
TCCAGTTACC GGGACAGGTC TTTCTCCCCT GGCTCCTATG AGAGGGAGAC CTTTGCCCTG 
GCCTCCTTGG AAACAGTCAC AGAAGCTCTC TTGGAGATCA TGGAGGCTTG TATGAGAGAT 
ATTCCTGCAT GCAAGTGGCT GGACCAGTGG ACAGAGCTAG CCCAGAGGTT TGCGTTCCAG 
TACAATCCCT CCCTGCAGCC ACGAGCTCTG GTTGTGTTTG GTTGTATCAG CAAAAGAGTG 
TCCCATGGGC AGATCAAGCA GATTATCCGC ATTCTTAGCA AGGGACTTGA GAGCTGCCTA 
AAAGGACCTG ATAATTATAA CAGTCAAGTT CTGATTGAAG CTACCGTGAT AGCTCTAACC 
AAATTACAAC CACTTCTTAA TAAGCAGGAG TCCCCTCTGC ACCGAGCTCT CTTCTGGGTG 
GCCGTTGCCG TGCTGCAGCT GGATGAAGTG GACCTGTACT CGGCGGGCAC TGCCCTCCTC 
GAACAGAACC TGCACACCTT AGACAGCCTC CTGCACATCT TCAACGACCA GAGCCCAGAA 
GAAGTGTTCA TGGAGATCCG GCAGCCTCTG GAGTGGCACT GCAAGCAGAT GGACCACTTT 
GTGGGGCTCA ACTTTAACTC CAGCTTTAAC TTTGCCCTGG TCGGGCACCT GCTGAAGGGA 
TTTCGGCATC CTTCACCTGT CACTGTTGCA AGAACTGTGA GGATTTTGCA CACCCTACTA 
GCATTGGTTA ACAAGCATAG GAATTGTGAC AAGTTTGAGG TGAACACCCA GAGTGTGGCT 
TACTTAGCAG CTTTACTTAC TGTGTCTGAG GAGGTTCGAA GTCGCTGCAG CTTAAAGCAT 
AGGAAATCAC TCCTACTAAC TGATATCACA ATGGAAAATG TGCCTATGGA TATATATCCC 
ATCTATCACA GTGACCCCAG CCTTAGGACA CTAAAAGAAA CACAACCGTG GTCCTCTCCA 
AAGGGATCTG AGAGACATCT TGCAGCCAGC TACCCTCCTG TGGGCCAAAC TAGCCCCCGA 
ACCAGAAAAT CCATGAGCCT GGATATGGGG CAGCCTTCCC AGGCCAACAC AAAGAAGTTG 
CTTGGGACAA GGAAAAGCTT CGATCATTTG ATCTCTGATG CCAAAGCTCC CAAAAGGCAA 
GAACAGGAGT CTGGAGTCAC CACGCCCCCC AAGATGCGGA GAGTGGCCGA GAACGACTAC 
GAGATGGAAA CCCAGAGAAT CTCCCCTCAG CCACACCCGC CTCTGCGGAA AGTGTCCGTG 
TCCGAATCCA ATGTGCTGCT GGACGAGGAA GTGCTCACAG ACCCCAAAAC CCAGGCTCTT 
CTTCTCACTG TCCTGGCTAC TCTGGTGAAG TACACTGCGG ACGAGTTTGA CCAGCGCATT 
CTCTATGAGT ACTTAGCGGA GGCCAGTGTT GTCTTCCCAA AGGTTTTTCC AGTGGTACAT 
AATTTGTTGG ACTCAAAGAT AAATACTCTG CTCTCATTAT GCCAAGACCC AAATTTGCTA 
AACCCGGTTC ATGGAATTGT GCAGAGTGTG GTCTACCACG AAGAGGCCCC GCCGCAGTAC 
CAGACGGCGT ACCTCCAGAG TTTCGGTTTT AATGGTCTGT GGAGGTTTGC AGGACCATTT 
TCTAAGCAGA CGCAGATCCC AGACTATGCC GAGCTCATCG TGAAGTTTCT CGATGCCTTG 
ATTGACACGT ATCTGCCTGG AATTGATGAG GAAGCCAGTG AGGAGTCCCT CCTGACACCC 
ACCTCTCCCT ACCCGCCCGC GGTGCAGAGC CAGCTTAGCA TCACTGCGAA CCTCAACCTC 
TCTAACTCCA TGACCTCACT GGCAACTTCC CAGCATTCCC CAGGCATCGA CAAGGAGAAT 
GTGGAGCTCT CGCCAACCAC CGGGCACTGC AGCGGCGGGA GGACGCGCCA CGGCTCCGCC 
AGCCAGGTCC AGAAGCAAAG GAGTGCCGGC AGCTTCAAGA GGCACAGCAT CAAGAAGATC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823507.1
CDS	9..8654
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKFSTLLTTITAWILSPWLCDTDVSIPLSCQITRPQSSFKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIVLGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKQESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967647.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967647.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGCCAAAG GACACGATGA GGTTAGATGA AACAATGCTG 
GTCAAACAGT TGCTGCCGGA AATCTGCCAT TTCCTTCACA CCTACCGGGA AGGGAACCAG 
CACGCAGCCG AGCTTCGGAG CTCCGCCTCT GGCGTCCTTT TCTCCCTCAG CTGTAATAAC 
TTCAATGCCG TCTTCAGCCG GATTTCCACC AGGTTACAGG AATTGACTGT TTGCTCAGAA 
GATAATGTTG ATGTCCATGA TATAGAATTA TTACAATATA TCAATGTGGA TTGTGCAAAA 
CTAAAGCGGC TCTTGCAGGA AACTGTATTT AAGTTTAAAG CCTTAAAGAA GGTAGCTCAA 
CTAGCAGTTA TAAATAGCCT AGAAAAGGCA TTTTGGAATT GGGTAGAGAA CTATCCTGAT 
GAATTTACAA AGCTGTACCA GACTCCCCAG ACAGATATGG CGGAATGTGC AGAAAAATTA 
TTTGACTTGG TGGATGGTTT TGCTGAAAGC ACCAAACGTA AAGCTGCAGT TTGGCCACTG 
CAAATCATTC TTCTTGTCTT GTGTCCAGAA ATAATCCAGG ACATTTCCAA GGATGTGGTT 
GATGAAGCGA AAATGAATAA GTTTTCTACA CTGTTGACCA CTATCACCGC CTGGATACTT 
TCTCCTTGGC TTTGTGATAC CGATGTCTCA ATTCCTCTTT CCTGCCAAAT CACTCGTCCT 
CAGTCCTCCT TTAAAATGTT TCTCGACAAT CTCAGAAGAG CGCTGGCCGG CCACAGTGGG 
AGCAGGCAGC TGACAGAGAG TGCCGCGATC GCTTGTGTTA AACTGTGCAA AGCCAGCACC 
TACATCAACT GGGAGGATAA CTCGGTCATT TTCCTGCTCG TCCAATCCAT GGTGGTGGAT 
CTGAAGAACT TGCTCTTTAA TCCCAGTAAG CCCTTCTCCA GAGGCAGCCA GAATGCTGAT 
GTGGACCTCA TGATAGACTG CCTGGTTTCT TGCTTTCGCA TCAGCCCTCA CAACAACCAA 
CACTTTAAGA TCTGTTTGGC CCAGAACTCC CCATCTACAT TTCATTATGT GCTGGTAAAC 
TCCCTCCATC GAATTATCAC CAATAGCACT TTCAAGCATG GACTTGGCAC TGCTTCCGCC 
CTGGATTGGT GGCCCAAGAT TGATGCTGTG TATTGCCATT CTGTAGAACT TCGAAACATG 
TTTGGTGAAA CGCTTCACAA GGCCGTGCAG GGCTGTGGGC CTCACCCCGC GATACGAATG 
GCGCCGAGTC TTACATTTAA AGAAAAAATG ACAAGCCTTA AATTTAAAGA AAAACCTACG 
GACTTGGAAA CAAGAAGTTA TAAATACCTC TTATTATCCA TGGTGAAACT AATCCATGCA 
GATCCAAAGC TGTTGCTATG TAATCCAAGA AAACAAGGGC CTGAAACTCA GGGGAGTACA 
GCAGAGTTAA TCACAGGCCT TGTGCAGCTC GTCCCACAGT CCAGCATGCC AGAAATAGCT 
CAAGAAGCAA TGGAGGCTTT GCTGGTTCTT CATCAGTTGG ATAGTATTGA CTTATGGAAT 
CCAGATTCAC CTATAGAAAC ATTCTGGGAG ATTAGCTCAC AAATGCTTTT TTATATCTGT 
AAAAAGCTAA CTACCCATCA GATGCTTAGC AGTACAGAAA TTCTCAAGTG GTTGCGAGAA 
ATTTTGATCT GTAGGAACAA ATATCTTCTT AAAAATAAGC AGTCAGATAG GAGTTCTTCC 
CATTTCCTCT ACCTTTATGG GGCAGGCTAT GATGTCTCTT CTGGTGGAGG TACCAGTCAT 
ATCTCTCTAG ACCACGAGGA GTTTATGCGT ACCTGTGCTC ATGGACCATC TCTCAGGAAA 
GTGAAAGGAA GCTCCTCAAT GGACGGTGCA ATCGGGTCCA GCGGGACGCC CATCTGCCGC 
CAGGCCCAGA CTAAGCTGGA GGTTGCTCTG TACATGTTTC TGTGGAGTCC CGACATTGAA 
GCCGTGCTCG TGGCCATGTC CTGCTTTCGA CACCTGTGTG AGGAAGCCGA CATCAGGTGT 
GGCATAGATG AGATCTCGGT GCACCATTTC TTGCCAAACT ATAACACCTT CATGGAGTTC 
GCATCTGTCA GCAACATGAT GTCGACAGGA AGAGCAGCTC TTCAGAAAAG AGTGATGGCC 
CTGCTCAGGC GCATCGAACA CCCCACCGCT GGAAACACCG AGGCTTGGGA AGATACTTAC 
GCCAAATGGG AGCAAGCTAC AAAATCAATC CTCAATTATC CGAAAGCCAA AATTGAAGAT 
GGCCAGGTTG CTGAGAGTCT TCACAAGACC ATTGTGAAGA GAAGAATGTC TCACGTGAGT 
GGAGGGGGCT CCATAGACTT GTGCGACACT GACTCGCTAC AGGAGTGGAT CAACATGACT 
GGGTTTCTCT GTGCCCTTGG TGGCGTGTGC CTACAGCAGC GGAGCAATGC CGGCTTTGCC 
ACCTACAGCC CTCCCATGGG CCCTATCAGT GAACGCAAGG GCTCTCTCAT TTCCATGGTG 
TCCAGCGATG GAAACGCAGA GACGCCTGTC AGCAAGTTCA TGGATCGGCT GCTCTCCTTG 
ATGGTGTGTA ACCACGAAAA AGTGGGGCTG CAGATACGAA GCAATGTCAA GGACTTGTTG 
GGTCTTGAAT TGAGTCCTGC CCTTTATCCA ATGCTGTTTA ACAAATTAAA AAATACCATC 
AGCAAGTTTT TTGATCCTCA AGGACAGGTT TTATTGACTG ACACCAACAC CCAGTTTGTG 
GAGCAGACCA TAGCCATAAT GAAGAACTTG CTTGATAATC ATGCTGAGGG TAGTTCTGAA 
CACATGGGAC AAGCTAGCAT CGAAACTATG ATGTTAAATC TGGTCAGATA TGTTAGAGTG 
TTTGGGAATT TGGTGCAGGC AATTCAGATC AAAACTAAGC TGTGCCAGCT GGTTCAAGTA 
ATGATGGAGA GGAGAGATGA CCTTTCATTT TGCCAAGAGA TGAAATTTAG GAATAAAATG 
GTAGAATATC TGACAGATTG GGTCATGGGA ACATCTAACC AAGCTGCAGA TGAAGATGTG 
AAATGTCTAA CTAGAGATTT GGACCAGGCA AGCATGGAAG CAGTGGTGTC ATTGCTCGCT 
GGGCTCCCTC TGCAGCCTGA GGAAGGCGAT GGGGTGGAAT TGATGGAAGC CAAATCCCAG 
CTGTTCCTTA AGTATTTCAC CCTCTTTATG AACCTTCTTA ATGACTGCAG TGAGGTCGAA 
GAAGAAAATG TCCAGATTGG GGGCAGGAAG CGCGGCATGT CGCGAAGGCT GGCGTCCCTG 
CGCCACTGCA CCGTGCTGGC AATGTCCAAC CTGCTCAATG CGAATGTAGA CAGCGGCCTG 
ATGCACTCTA TAGTGCTAGG ATTGGGTTAC CACAAAGATC TTCAGACAAG AGCCACCTTC 
ATGGAAGTCC TCACAAAAAT CCTTCAGCAA GGCACAGAGT TTGATACGCT CGCAGAAACC 
GTGTTAGCTG ATCGATTTGA GAGACTTGTT GAGTTGGTCA CGATGATGGG TGATCAAGGA 
GAGCTCCCCA TAGCCATGGC TCTGGCCAAC GTGGTGCCTT GTTCTCAGTG GGATGAACTT 
GCTCGAGTCC TTGTTACTCT CTTTGATTCC CGACACTTAC TGTATCAGCT CCTTTGGAAC 
ATGTTTTCTA AGGAAGTTGA GCTGGCAGAC TCCATGCAGA CACTTTTTAG AGGAAACAGC 
TTGGCCAGTA AAATCATGAC CTTCTGCTTT AAGGTCTACG GTGCTACATA CCTGCAGAAG 
CTTCTGGAGC CTTTACTCCG CTTTGTGATC ACATCCTCCG AATGGCAGCA CGTCAGCTTC 
GAGGTCGATC CCACCAGGTT AGAGCATACT GAGAGCCTCG AGGACAACCA GCGGAACCTT 
TTGCAAATGA CTGAGAAGTT CTTCCACGCC ATCATCAGTT CTTCCTCCGA GTTCCCCCCT 
CAGCTACGCA GTGTGTGCCA CTGCCTGTAC CAGGCAACTT GCCACTCTCT ACTGAATAAA 
GCTACCGTAA AAGAAAAAAA GGAAAACAAA AAATCAGTTG TTAGCCAGAG ATTCCCTCAG 
AACAGCATTG GCGCCGTGGG GAGTGCCATG TTCCTGAGAT TCATCAACCC TGCCGTTGTC 
TCTCCCTACG AGGCAGGGAT TTTAGAGAGA AAACCGCTCC CTAGAATTGA AAGGGGGTTG 
AAGCTGATGT CAAAGATTCT TCAGAGCATT GCCAATCATG TCCTTTTCAC AAAAGAAGAG 
TATATGAGGC CTTTTAACGA TTTTGTGAAA AGCAACTTTG ATGTAGCACG GAGATTTTTC 
CTTGATATCG CATCTGATTG TCCTGTAAGT GATGCCATAA ACCACAGCCT TTCCTTCATC 
AGCGATGGCA ATGTCTTGGC TTTGCACCGT CTGCTCTGGA ACAACCAAGA GAAAATCGGC 
CAATATCTTT CAAGCAACAG AGATCACAAA GCTGTTGGAA GGCGACCTTT CGATAAGATG 
GCAACACTGC TTGCCTACCT GGGACCTCCA GAGCACAAGC CTGTGGCTGA CACACACTGG 
TCCAGCCTAA ATCTTACCAG CTCCAAGTTT GAGGAATTCA TGACAAGGCA CCAGGTACAT 
GAAAAAGAGG AATTCAAGGC TCTGAAAACA TTAAATATCT TCTATCAAGC AGGCACTTCC 
AAAGCTGGAA ATCCTGTTTT TTATTATGTC ACACGAAGGT TCAAGACTGG CCAGATCAAC 
GGGGATTTGC TTATATACCA CGTCTTACTA ACTTTAAAGC CATATTATGC AAAGCCATAT 
GAGATTGTAA TGGACCTCAC TCATGCCGGG CCTAGCAATC GCTTTAAGAC AGACTTCCTC 
TCCAAGTGGT TTGTTGTTTT CCCGGGCTTT GCTTACGAGA ACGTCTCTTC GGTCTACATC 
TACAACTGTA ATTCTTGGGT TCGGGAGTAC ACCAAATACC ATGAGAGACT GCTCACGGGA 
CTCAAAGGCA GCAAAAGACT CTTTTTCATA GACTCTCCCT CAAAACTGGC TGAGCATATC 
GATTATGATC AGCAAAAACT GCCAGCCGCG ACCTTGGCTC TGGATGAAGA CCTCAAGGTG 
TACAACAGTG CTCTCAAGCT GGCTCACAAG GACACCAAGG TGACCCTCAA GGTTGGCTCT 
ACTGCGATCC AAGTGACGTC ATCTGAGCGA ACTAAAGTCC TGGGACAGAC GGTTTTTCTG 
AATGATATTT ACTATGCCTC TGAGATCGAA GAAATCTGTC TTGTGGATGA AAACCAGTTC 
ACTCTGACCA TTTCCAATCA GGGCACTCCC CTCACCTTCA TGCATCAAGA GTGTGAAGGC 
ATTGTGGCGT CCATCATTCA CATCCGAACT CGATGGGAGC TGTCCCAGCC AGATTCTATC 
CCCCAGCACA CTAAGATCCG GCCAAAGGAC GTTCCCGGAA CACTTTTGAA TATCGCATTA 
CTTAACTTGG GTAGTTCGGA CCCAAGTTTA CGGTCAGCCG CCTATAATCT TCTCTGTGCC 
TTAACCTGTA CCTTTAATTT AAAAATTGAG GGCCAATTAC TAGAAACATC AGGTCTGTGT 
ATACCTGCCA ACAATACCCT TTTTATTGTC TCTATTAGTA AAACCCTGGC AGCTAATGAG 
CCACACCTCA CCTTAGAGTT TTTGGAAGAG TGCATTTCCG GATTTAGCAA ATCCAGTATT 
GAACTGAAGC ACCTGTGTCT AGAATACATG ACTCCCTGGC TATCAAATCT AGTCCGTTTC 
TGTAAACATA ATGATGATGC CAAACGACAG AGAGTCACTG CTATTCTTGA TAAACTTATA 
ACAATGACCA TAAATGAAAA GCAGATGTAC CCATCGATCC AAGCCAAGAT ATGGGGAAGC 
CTTGGGCAGA TTACAGATCT GCTCGATGTA GTGCTGGACA GCTTTATCAA AACCAGTGCC 
ACAGGTGGTT TAGGATCCAT CAAGGCTGAA GTGATGGCTG ACACGGCCGT GGCTCTGGCT 
TCTGGGAACG TCAAACTAGT TTCGAGCAAG GTGATTGGAA GAATGTGCAA AATCATTGAC 
AAGACCTGCC TCTCTCCAAC TCCTACCTTA GAGCAGCACC TCATGTGGGA TGATATCGCC 
ATTTTAGCAC GCTACATGCT AATGCTGTCC TTCAATAATT CTCTGGATGT GGCGGCTCAT 
CTTCCCTACC TCTTCCATGT GGTGACTCTG TTGGTGGCCA CAGGTCCCTT GTCCCTTCGT 
GCTTCCACAC ACGGACTGGT CATCAATATC ATCCATTCTC TGTGCACGTG CTCACAGCTT 
CACTTTAGTG AAGAGACCAA GCAAGTTTTA CGACTGAGTC TGACTGAGTT CTCACTACCT 
AAGTTTTATT TGCTGTTCGG CATCAGCAAA GTCAAATCAG CTGCCGTGAT AGCCTTCCGG 
TCCAGTTACC GGGACAGGTC TTTCTCCCCT GGCTCCTATG AGAGGGAGAC CTTTGCCCTG 
GCCTCCTTGG AAACAGTCAC AGAAGCTCTC TTGGAGATCA TGGAGGCTTG TATGAGAGAT 
ATTCCTGCAT GCAAGTGGCT GGACCAGTGG ACAGAGCTAG CCCAGAGGTT TGCGTTCCAG 
TACAATCCCT CCCTGCAGCC ACGAGCTCTG GTTGTGTTTG GTTGTATCAG CAAAAGAGTG 
TCCCATGGGC AGATCAAGCA GATTATCCGC ATTCTTAGCA AGGGACTTGA GAGCTGCCTA 
AAAGGACCTG ATAATTATAA CAGTCAAGTT CTGATTGAAG CTACCGTGAT AGCTCTAACC 
AAATTACAAC CACTTCTTAA TAAGCAGGAG TCCCCTCTGC ACCGAGCTCT CTTCTGGGTG 
GCCGTTGCCG TGCTGCAGCT GGATGAAGTG GACCTGTACT CGGCGGGCAC TGCCCTCCTC 
GAACAGAACC TGCACACCTT AGACAGCCTC CTGCACATCT TCAACGACCA GAGCCCAGAA 
GAAGTGTTCA TGGAGATCCG GCAGCCTCTG GAGTGGCACT GCAAGCAGAT GGACCACTTT 
GTGGGGCTCA ACTTTAACTC CAGCTTTAAC TTTGCCCTGG TCGGGCACCT GCTGAAGGGA 
TTTCGGCATC CTTCACCTGT CACTGTTGCA AGAACTGTGA GGATTTTGCA CACCCTACTA 
GCATTGGTTA ACAAGCATAG GAATTGTGAC AAGTTTGAGG TGAACACCCA GAGTGTGGCT 
TACTTAGCAG CTTTACTTAC TGTGTCTGAG GAGGTTCGAA GTCGCTGCAG CTTAAAGCAT 
AGGAAATCAC TCCTACTAAC TGATATCACA ATGGAAAATG TGCCTATGGA TATATATCCC 
ATCTATCACA GTGACCCCAG CCTTAGGACA CTAAAAGAAA CACAACCGTG GTCCTCTCCA 
AAGGGATCTG AGAGACATCT TGCAGCCAGC TACCCTCCTG TGGGCCAAAC TAGCCCCCGA 
ACCAGAAAAT CCATGAGCCT GGATATGGGG CAGCCTTCCC AGGCCAACAC AAAGAAGTTG 
CTTGGGACAA GGAAAAGCTT CGATCATTTG ATCTCTGATG CCAAAGCTCC CAAAAGGCAA 
GAACAGGAGT CTGGAGTCAC CACGCCCCCC AAGATGCGGA GAGTGGCCGA GAACGACTAC 
GAGATGGAAA CCCAGAGAAT CTCCCCTCAG CCACACCCGC CTCTGCGGAA AGTGTCCGTG 
TCCGAATCCA ATGTGCTGCT GGACGAGGAA GTGCTCACAG ACCCCAAAAC CCAGGCTCTT 
CTTCTCACTG TCCTGGCTAC TCTGGTGAAG TACACTGCGG ACGAGTTTGA CCAGCGCATT 
CTCTATGAGT ACTTAGCGGA GGCCAGTGTT GTCTTCCCAA AGGTTTTTCC AGTGGTACAT 
AATTTGTTGG ACTCAAAGAT AAATACTCTG CTCTCATTAT GCCAAGACCC AAATTTGCTA 
AACCCGGTTC ATGGAATTGT GCAGAGTGTG GTCTACCACG AAGAGGCCCC GCCGCAGTAC 
CAGACGGCGT ACCTCCAGAG TTTCGGTTTT AATGGTCTGT GGAGGTTTGC AGGACCATTT 
TCTAAGCAGA CGCAGATCCC AGACTATGCC GAGCTCATCG TGAAGTTTCT CGATGCCTTG 
ATTGACACGT ATCTGCCTGG AATTGATGAG GAAGCCAGTG AGGAGTCCCT CCTGACACCC 
ACCTCTCCCT ACCCGCCCGC GGTGCAGAGC CAGCTTAGCA TCACTGCGAA CCTCAACCTC 
TCTAACTCCA TGACCTCACT GGCAACTTCC CAGCATTCCC CAGGCATCGA CAAGGAGAAT 
GTGGAGCTCT CGCCAACCAC CGGGCACTGC AGCGGCGGGA GGACGCGCCA CGGCTCCGCC 
AGCCAGGTCC AGAAGCAAAG GAGTGCCGGC AGCTTCAAGA GGCACAGCAT CAAGAAGATC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967648.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823508.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X9, mRNA.

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CloneID	OSa234696
Clone ID Related Accession (Same CDS sequence)	XM_031967648.1
Accession Version	XM_031967648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATT CCGCCCTGGA TTGGTGGCCC 
AAGATTGATG CTGTGTATTG CCATTCTGTA GAACTTCGAA ACATGTTTGG TGAAACGCTT 
CACAAGGCCG TGCAGGGCTG TGGGCCTCAC CCCGCGATAC GAATGGCGCC GAGTCTTACA 
TTTAAAGAAA AAATGACAAG CCTTAAATTT AAAGAAAAAC CTACGGACTT GGAAACAAGA 
AGTTATAAAT ACCTCTTATT ATCCATGGTG AAACTAATCC ATGCAGATCC AAAGCTGTTG 
CTATGTAATC CAAGAAAACA AGGGCCTGAA ACTCAGGGGA GTACAGCAGA GTTAATCACA 
GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC ATGCCAGAAA TAGCTCAAGA AGCAATGGAG 
GCTTTGCTGG TTCTTCATCA GTTGGATAGT ATTGACTTAT GGAATCCAGA TTCACCTATA 
GAAACATTCT GGGAGATTAG CTCACAAATG CTTTTTTATA TCTGTAAAAA GCTAACTACC 
CATCAGATGC TTAGCAGTAC AGAAATTCTC AAGTGGTTGC GAGAAATTTT GATCTGTAGG 
AACAAATATC TTCTTAAAAA TAAGCAGTCA GATAGGAGTT CTTCCCATTT CCTCTACCTT 
TATGGGGCAG GCTATGATGT CTCTTCTGGT GGAGGTACCA GTCATATCTC TCTAGACCAC 
GAGGAGTTTA TGCGTACCTG TGCTCATGGA CCATCTCTCA GGAAAGTGAA AGGAAGCTCC 
TCAATGGACG GTGCAATCGG GTCCAGCGGG ACGCCCATCT GCCGCCAGGC CCAGACTAAG 
CTGGAGGTTG CTCTGTACAT GTTTCTGTGG AGTCCCGACA TTGAAGCCGT GCTCGTGGCC 
ATGTCCTGCT TTCGACACCT GTGTGAGGAA GCCGACATCA GGTGTGGCAT AGATGAGATC 
TCGGTGCACC ATTTCTTGCC AAACTATAAC ACCTTCATGG AGTTCGCATC TGTCAGCAAC 
ATGATGTCGA CAGGAAGAGC AGCTCTTCAG AAAAGAGTGA TGGCCCTGCT CAGGCGCATC 
GAACACCCCA CCGCTGGAAA CACCGAGGCT TGGGAAGATA CTTACGCCAA ATGGGAGCAA 
GCTACAAAAT CAATCCTCAA TTATCCGAAA GCCAAAATTG AAGATGGCCA GGTTGCTGAG 
AGTCTTCACA AGACCATTGT GAAGAGAAGA ATGTCTCACG TGAGTGGAGG GGGCTCCATA 
GACTTGTGCG ACACTGACTC GCTACAGGAG TGGATCAACA TGACTGGGTT TCTCTGTGCC 
CTTGGTGGCG TGTGCCTACA GCAGCGGAGC AATGCCGGCT TTGCCACCTA CAGCCCTCCC 
ATGGGCCCTA TCAGTGAACG CAAGGGCTCT CTCATTTCCA TGGTGTCCAG CGATGGAAAC 
GCAGAGACGC CTGTCAGCAA GTTCATGGAT CGGCTGCTCT CCTTGATGGT GTGTAACCAC 
GAAAAAGTGG GGCTGCAGAT ACGAAGCAAT GTCAAGGACT TGTTGGGTCT TGAATTGAGT 
CCTGCCCTTT ATCCAATGCT GTTTAACAAA TTAAAAAATA CCATCAGCAA GTTTTTTGAT 
CCTCAAGGAC AGGTTTTATT GACTGACACC AACACCCAGT TTGTGGAGCA GACCATAGCC 
ATAATGAAGA ACTTGCTTGA TAATCATGCT GAGGGTAGTT CTGAACACAT GGGACAAGCT 
AGCATCGAAA CTATGATGTT AAATCTGGTC AGATATGTTA GAGTGTTTGG GAATTTGGTG 
CAGGCAATTC AGATCAAAAC TAAGCTGTGC CAGCTGGTTC AAGTAATGAT GGAGAGGAGA 
GATGACCTTT CATTTTGCCA AGAGATGAAA TTTAGGAATA AAATGGTAGA ATATCTGACA 
GATTGGGTCA TGGGAACATC TAACCAAGCT GCAGATGAAG ATGTGAAATG TCTAACTAGA 
GATTTGGACC AGGCAAGCAT GGAAGCAGTG GTGTCATTGC TCGCTGGGCT CCCTCTGCAG 
CCTGAGGAAG GCGATGGGGT GGAATTGATG GAAGCCAAAT CCCAGCTGTT CCTTAAGTAT 
TTCACCCTCT TTATGAACCT TCTTAATGAC TGCAGTGAGG TCGAAGAAGA AAATGTCCAG 
ATTGGGGGCA GGAAGCGCGG CATGTCGCGA AGGCTGGCGT CCCTGCGCCA CTGCACCGTG 
CTGGCAATGT CCAACCTGCT CAATGCGAAT GTAGACAGCG GCCTGATGCA CTCTATAGTG 
CTAGGATTGG GTTACCACAA AGATCTTCAG ACAAGAGCCA CCTTCATGGA AGTCCTCACA 
AAAATCCTTC AGCAAGGCAC AGAGTTTGAT ACGCTCGCAG AAACCGTGTT AGCTGATCGA 
TTTGAGAGAC TTGTTGAGTT GGTCACGATG ATGGGTGATC AAGGAGAGCT CCCCATAGCC 
ATGGCTCTGG CCAACGTGGT GCCTTGTTCT CAGTGGGATG AACTTGCTCG AGTCCTTGTT 
ACTCTCTTTG ATTCCCGACA CTTACTGTAT CAGCTCCTTT GGAACATGTT TTCTAAGGAA 
GTTGAGCTGG CAGACTCCAT GCAGACACTT TTTAGAGGAA ACAGCTTGGC CAGTAAAATC 
ATGACCTTCT GCTTTAAGGT CTACGGTGCT ACATACCTGC AGAAGCTTCT GGAGCCTTTA 
CTCCGCTTTG TGATCACATC CTCCGAATGG CAGCACGTCA GCTTCGAGGT CGATCCCACC 
AGGTTAGAGC ATACTGAGAG CCTCGAGGAC AACCAGCGGA ACCTTTTGCA AATGACTGAG 
AAGTTCTTCC ACGCCATCAT CAGTTCTTCC TCCGAGTTCC CCCCTCAGCT ACGCAGTGTG 
TGCCACTGCC TGTACCAGGC AACTTGCCAC TCTCTACTGA ATAAAGCTAC CGTAAAAGAA 
AAAAAGGAAA ACAAAAAATC AGTTGTTAGC CAGAGATTCC CTCAGAACAG CATTGGCGCC 
GTGGGGAGTG CCATGTTCCT GAGATTCATC AACCCTGCCG TTGTCTCTCC CTACGAGGCA 
GGGATTTTAG AGAGAAAACC GCTCCCTAGA ATTGAAAGGG GGTTGAAGCT GATGTCAAAG 
ATTCTTCAGA GCATTGCCAA TCATGTCCTT TTCACAAAAG AAGAGTATAT GAGGCCTTTT 
AACGATTTTG TGAAAAGCAA CTTTGATGTA GCACGGAGAT TTTTCCTTGA TATCGCATCT 
GATTGTCCTG TAAGTGATGC CATAAACCAC AGCCTTTCCT TCATCAGCGA TGGCAATGTC 
TTGGCTTTGC ACCGTCTGCT CTGGAACAAC CAAGAGAAAA TCGGCCAATA TCTTTCAAGC 
AACAGAGATC ACAAAGCTGT TGGAAGGCGA CCTTTCGATA AGATGGCAAC ACTGCTTGCC 
TACCTGGGAC CTCCAGAGCA CAAGCCTGTG GCTGACACAC ACTGGTCCAG CCTAAATCTT 
ACCAGCTCCA AGTTTGAGGA ATTCATGACA AGGCACCAGG TACATGAAAA AGAGGAATTC 
AAGGCTCTGA AAACATTAAA TATCTTCTAT CAAGCAGGCA CTTCCAAAGC TGGAAATCCT 
GTTTTTTATT ATGTCACACG AAGGTTCAAG ACTGGCCAGA TCAACGGGGA TTTGCTTATA 
TACCACGTCT TACTAACTTT AAAGCCATAT TATGCAAAGC CATATGAGAT TGTAATGGAC 
CTCACTCATG CCGGGCCTAG CAATCGCTTT AAGACAGACT TCCTCTCCAA GTGGTTTGTT 
GTTTTCCCGG GCTTTGCTTA CGAGAACGTC TCTTCGGTCT ACATCTACAA CTGTAATTCT 
TGGGTTCGGG AGTACACCAA ATACCATGAG AGACTGCTCA CGGGACTCAA AGGCAGCAAA 
AGACTCTTTT TCATAGACTC TCCCTCAAAA CTGGCTGAGC ATATCGATTA TGATCAGCAA 
AAACTGCCAG CCGCGACCTT GGCTCTGGAT GAAGACCTCA AGGTGTACAA CAGTGCTCTC 
AAGCTGGCTC ACAAGGACAC CAAGGTGACC CTCAAGGTTG GCTCTACTGC GATCCAAGTG 
ACGTCATCTG AGCGAACTAA AGTCCTGGGA CAGACGGTTT TTCTGAATGA TATTTACTAT 
GCCTCTGAGA TCGAAGAAAT CTGTCTTGTG GATGAAAACC AGTTCACTCT GACCATTTCC 
AATCAGGGCA CTCCCCTCAC CTTCATGCAT CAAGAGTGTG AAGGCATTGT GGCGTCCATC 
ATTCACATCC GAACTCGATG GGAGCTGTCC CAGCCAGATT CTATCCCCCA GCACACTAAG 
ATCCGGCCAA AGGACGTTCC CGGAACACTT TTGAATATCG CATTACTTAA CTTGGGTAGT 
TCGGACCCAA GTTTACGGTC AGCCGCCTAT AATCTTCTCT GTGCCTTAAC CTGTACCTTT 
AATTTAAAAA TTGAGGGCCA ATTACTAGAA ACATCAGGTC TGTGTATACC TGCCAACAAT 
ACCCTTTTTA TTGTCTCTAT TAGTAAAACC CTGGCAGCTA ATGAGCCACA CCTCACCTTA 
GAGTTTTTGG AAGAGTGCAT TTCCGGATTT AGCAAATCCA GTATTGAACT GAAGCACCTG 
TGTCTAGAAT ACATGACTCC CTGGCTATCA AATCTAGTCC GTTTCTGTAA ACATAATGAT 
GATGCCAAAC GACAGAGAGT CACTGCTATT CTTGATAAAC TTATAACAAT GACCATAAAT 
GAAAAGCAGA TGTACCCATC GATCCAAGCC AAGATATGGG GAAGCCTTGG GCAGATTACA 
GATCTGCTCG ATGTAGTGCT GGACAGCTTT ATCAAAACCA GTGCCACAGG TGGTTTAGGA 
TCCATCAAGG CTGAAGTGAT GGCTGACACG GCCGTGGCTC TGGCTTCTGG GAACGTCAAA 
CTAGTTTCGA GCAAGGTGAT TGGAAGAATG TGCAAAATCA TTGACAAGAC CTGCCTCTCT 
CCAACTCCTA CCTTAGAGCA GCACCTCATG TGGGATGATA TCGCCATTTT AGCACGCTAC 
ATGCTAATGC TGTCCTTCAA TAATTCTCTG GATGTGGCGG CTCATCTTCC CTACCTCTTC 
CATGTGGTGA CTCTGTTGGT GGCCACAGGT CCCTTGTCCC TTCGTGCTTC CACACACGGA 
CTGGTCATCA ATATCATCCA TTCTCTGTGC ACGTGCTCAC AGCTTCACTT TAGTGAAGAG 
ACCAAGCAAG TTTTACGACT GAGTCTGACT GAGTTCTCAC TACCTAAGTT TTATTTGCTG 
TTCGGCATCA GCAAAGTCAA ATCAGCTGCC GTGATAGCCT TCCGGTCCAG TTACCGGGAC 
AGGTCTTTCT CCCCTGGCTC CTATGAGAGG GAGACCTTTG CCCTGGCCTC CTTGGAAACA 
GTCACAGAAG CTCTCTTGGA GATCATGGAG GCTTGTATGA GAGATATTCC TGCATGCAAG 
TGGCTGGACC AGTGGACAGA GCTAGCCCAG AGGTTTGCGT TCCAGTACAA TCCCTCCCTG 
CAGCCACGAG CTCTGGTTGT GTTTGGTTGT ATCAGCAAAA GAGTGTCCCA TGGGCAGATC 
AAGCAGATTA TCCGCATTCT TAGCAAGGGA CTTGAGAGCT GCCTAAAAGG ACCTGATAAT 
TATAACAGTC AAGTTCTGAT TGAAGCTACC GTGATAGCTC TAACCAAATT ACAACCACTT 
CTTAATAAGC AGGAGTCCCC TCTGCACCGA GCTCTCTTCT GGGTGGCCGT TGCCGTGCTG 
CAGCTGGATG AAGTGGACCT GTACTCGGCG GGCACTGCCC TCCTCGAACA GAACCTGCAC 
ACCTTAGACA GCCTCCTGCA CATCTTCAAC GACCAGAGCC CAGAAGAAGT GTTCATGGAG 
ATCCGGCAGC CTCTGGAGTG GCACTGCAAG CAGATGGACC ACTTTGTGGG GCTCAACTTT 
AACTCCAGCT TTAACTTTGC CCTGGTCGGG CACCTGCTGA AGGGATTTCG GCATCCTTCA 
CCTGTCACTG TTGCAAGAAC TGTGAGGATT TTGCACACCC TACTAGCATT GGTTAACAAG 
CATAGGAATT GTGACAAGTT TGAGGTGAAC ACCCAGAGTG TGGCTTACTT AGCAGCTTTA 
CTTACTGTGT CTGAGGAGGT TCGAAGTCGC TGCAGCTTAA AGCATAGGAA ATCACTCCTA 
CTAACTGATA TCACAATGGA AAATGTGCCT ATGGATATAT ATCCCATCTA TCACAGTGAC 
CCCAGCCTTA GGACACTAAA AGAAACACAA CCGTGGTCCT CTCCAAAGGG ATCTGAGAGA 
CATCTTGCAG CCAGCTACCC TCCTGTGGGC CAAACTAGCC CCCGAACCAG AAAATCCATG 
AGCCTGGATA TGGGGCAGCC TTCCCAGGCC AACACAAAGA AGTTGCTTGG GACAAGGAAA 
AGCTTCGATC ATTTGATCTC TGATGCCAAA GCTCCCAAAA GGCAAGAACA GGAGTCTGGA 
GTCACCACGC CCCCCAAGAT GCGGAGAGTG GCCGAGAACG ACTACGAGAT GGAAACCCAG 
AGAATCTCCC CTCAGCCACA CCCGCCTCTG CGGAAAGTGT CCGTGTCCGA ATCCAATGTG 
CTGCTGGACG AGGAAGTGCT CACAGACCCC AAAACCCAGG CTCTTCTTCT CACTGTCCTG 
GCTACTCTGG TGAAGTACAC TGCGGACGAG TTTGACCAGC GCATTCTCTA TGAGTACTTA 
GCGGAGGCCA GTGTTGTCTT CCCAAAGGTT TTTCCAGTGG TACATAATTT GTTGGACTCA 
AAGATAAATA CTCTGCTCTC ATTATGCCAA GACCCAAATT TGCTAAACCC GGTTCATGGA 
ATTGTGCAGA GTGTGGTCTA CCACGAAGAG GCCCCGCCGC AGTACCAGAC GGCGTACCTC 
CAGAGTTTCG GTTTTAATGG TCTGTGGAGG TTTGCAGGAC CATTTTCTAA GCAGACGCAG 
ATCCCAGACT ATGCCGAGCT CATCGTGAAG TTTCTCGATG CCTTGATTGA CACGTATCTG 
CCTGGAATTG ATGAGGAAGC CAGTGAGGAG TCCCTCCTGA CACCCACCTC TCCCTACCCG 
CCCGCGGTGC AGAGCCAGCT TAGCATCACT GCGAACCTCA ACCTCTCTAA CTCCATGACC 
TCACTGGCAA CTTCCCAGCA TTCCCCAGGC ATCGACAAGG AGAATGTGGA GCTCTCGCCA 
ACCACCGGGC ACTGCAGCGG CGGGAGGACG CGCCACGGCT CCGCCAGCCA GGTCCAGAAG 
CAAAGGAGTG CCGGCAGCTT CAAGAGGCAC AGCATCAAGA AGATCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823508.1
CDS	9..8639
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGVSDLQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKFSTLLTTITAWILSPWLCDTDVSIPLSCQITRPQSSFKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIVLGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKQESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967648.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967648.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATT CCGCCCTGGA TTGGTGGCCC 
AAGATTGATG CTGTGTATTG CCATTCTGTA GAACTTCGAA ACATGTTTGG TGAAACGCTT 
CACAAGGCCG TGCAGGGCTG TGGGCCTCAC CCCGCGATAC GAATGGCGCC GAGTCTTACA 
TTTAAAGAAA AAATGACAAG CCTTAAATTT AAAGAAAAAC CTACGGACTT GGAAACAAGA 
AGTTATAAAT ACCTCTTATT ATCCATGGTG AAACTAATCC ATGCAGATCC AAAGCTGTTG 
CTATGTAATC CAAGAAAACA AGGGCCTGAA ACTCAGGGGA GTACAGCAGA GTTAATCACA 
GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC ATGCCAGAAA TAGCTCAAGA AGCAATGGAG 
GCTTTGCTGG TTCTTCATCA GTTGGATAGT ATTGACTTAT GGAATCCAGA TTCACCTATA 
GAAACATTCT GGGAGATTAG CTCACAAATG CTTTTTTATA TCTGTAAAAA GCTAACTACC 
CATCAGATGC TTAGCAGTAC AGAAATTCTC AAGTGGTTGC GAGAAATTTT GATCTGTAGG 
AACAAATATC TTCTTAAAAA TAAGCAGTCA GATAGGAGTT CTTCCCATTT CCTCTACCTT 
TATGGGGCAG GCTATGATGT CTCTTCTGGT GGAGGTACCA GTCATATCTC TCTAGACCAC 
GAGGAGTTTA TGCGTACCTG TGCTCATGGA CCATCTCTCA GGAAAGTGAA AGGAAGCTCC 
TCAATGGACG GTGCAATCGG GTCCAGCGGG ACGCCCATCT GCCGCCAGGC CCAGACTAAG 
CTGGAGGTTG CTCTGTACAT GTTTCTGTGG AGTCCCGACA TTGAAGCCGT GCTCGTGGCC 
ATGTCCTGCT TTCGACACCT GTGTGAGGAA GCCGACATCA GGTGTGGCAT AGATGAGATC 
TCGGTGCACC ATTTCTTGCC AAACTATAAC ACCTTCATGG AGTTCGCATC TGTCAGCAAC 
ATGATGTCGA CAGGAAGAGC AGCTCTTCAG AAAAGAGTGA TGGCCCTGCT CAGGCGCATC 
GAACACCCCA CCGCTGGAAA CACCGAGGCT TGGGAAGATA CTTACGCCAA ATGGGAGCAA 
GCTACAAAAT CAATCCTCAA TTATCCGAAA GCCAAAATTG AAGATGGCCA GGTTGCTGAG 
AGTCTTCACA AGACCATTGT GAAGAGAAGA ATGTCTCACG TGAGTGGAGG GGGCTCCATA 
GACTTGTGCG ACACTGACTC GCTACAGGAG TGGATCAACA TGACTGGGTT TCTCTGTGCC 
CTTGGTGGCG TGTGCCTACA GCAGCGGAGC AATGCCGGCT TTGCCACCTA CAGCCCTCCC 
ATGGGCCCTA TCAGTGAACG CAAGGGCTCT CTCATTTCCA TGGTGTCCAG CGATGGAAAC 
GCAGAGACGC CTGTCAGCAA GTTCATGGAT CGGCTGCTCT CCTTGATGGT GTGTAACCAC 
GAAAAAGTGG GGCTGCAGAT ACGAAGCAAT GTCAAGGACT TGTTGGGTCT TGAATTGAGT 
CCTGCCCTTT ATCCAATGCT GTTTAACAAA TTAAAAAATA CCATCAGCAA GTTTTTTGAT 
CCTCAAGGAC AGGTTTTATT GACTGACACC AACACCCAGT TTGTGGAGCA GACCATAGCC 
ATAATGAAGA ACTTGCTTGA TAATCATGCT GAGGGTAGTT CTGAACACAT GGGACAAGCT 
AGCATCGAAA CTATGATGTT AAATCTGGTC AGATATGTTA GAGTGTTTGG GAATTTGGTG 
CAGGCAATTC AGATCAAAAC TAAGCTGTGC CAGCTGGTTC AAGTAATGAT GGAGAGGAGA 
GATGACCTTT CATTTTGCCA AGAGATGAAA TTTAGGAATA AAATGGTAGA ATATCTGACA 
GATTGGGTCA TGGGAACATC TAACCAAGCT GCAGATGAAG ATGTGAAATG TCTAACTAGA 
GATTTGGACC AGGCAAGCAT GGAAGCAGTG GTGTCATTGC TCGCTGGGCT CCCTCTGCAG 
CCTGAGGAAG GCGATGGGGT GGAATTGATG GAAGCCAAAT CCCAGCTGTT CCTTAAGTAT 
TTCACCCTCT TTATGAACCT TCTTAATGAC TGCAGTGAGG TCGAAGAAGA AAATGTCCAG 
ATTGGGGGCA GGAAGCGCGG CATGTCGCGA AGGCTGGCGT CCCTGCGCCA CTGCACCGTG 
CTGGCAATGT CCAACCTGCT CAATGCGAAT GTAGACAGCG GCCTGATGCA CTCTATAGTG 
CTAGGATTGG GTTACCACAA AGATCTTCAG ACAAGAGCCA CCTTCATGGA AGTCCTCACA 
AAAATCCTTC AGCAAGGCAC AGAGTTTGAT ACGCTCGCAG AAACCGTGTT AGCTGATCGA 
TTTGAGAGAC TTGTTGAGTT GGTCACGATG ATGGGTGATC AAGGAGAGCT CCCCATAGCC 
ATGGCTCTGG CCAACGTGGT GCCTTGTTCT CAGTGGGATG AACTTGCTCG AGTCCTTGTT 
ACTCTCTTTG ATTCCCGACA CTTACTGTAT CAGCTCCTTT GGAACATGTT TTCTAAGGAA 
GTTGAGCTGG CAGACTCCAT GCAGACACTT TTTAGAGGAA ACAGCTTGGC CAGTAAAATC 
ATGACCTTCT GCTTTAAGGT CTACGGTGCT ACATACCTGC AGAAGCTTCT GGAGCCTTTA 
CTCCGCTTTG TGATCACATC CTCCGAATGG CAGCACGTCA GCTTCGAGGT CGATCCCACC 
AGGTTAGAGC ATACTGAGAG CCTCGAGGAC AACCAGCGGA ACCTTTTGCA AATGACTGAG 
AAGTTCTTCC ACGCCATCAT CAGTTCTTCC TCCGAGTTCC CCCCTCAGCT ACGCAGTGTG 
TGCCACTGCC TGTACCAGGC AACTTGCCAC TCTCTACTGA ATAAAGCTAC CGTAAAAGAA 
AAAAAGGAAA ACAAAAAATC AGTTGTTAGC CAGAGATTCC CTCAGAACAG CATTGGCGCC 
GTGGGGAGTG CCATGTTCCT GAGATTCATC AACCCTGCCG TTGTCTCTCC CTACGAGGCA 
GGGATTTTAG AGAGAAAACC GCTCCCTAGA ATTGAAAGGG GGTTGAAGCT GATGTCAAAG 
ATTCTTCAGA GCATTGCCAA TCATGTCCTT TTCACAAAAG AAGAGTATAT GAGGCCTTTT 
AACGATTTTG TGAAAAGCAA CTTTGATGTA GCACGGAGAT TTTTCCTTGA TATCGCATCT 
GATTGTCCTG TAAGTGATGC CATAAACCAC AGCCTTTCCT TCATCAGCGA TGGCAATGTC 
TTGGCTTTGC ACCGTCTGCT CTGGAACAAC CAAGAGAAAA TCGGCCAATA TCTTTCAAGC 
AACAGAGATC ACAAAGCTGT TGGAAGGCGA CCTTTCGATA AGATGGCAAC ACTGCTTGCC 
TACCTGGGAC CTCCAGAGCA CAAGCCTGTG GCTGACACAC ACTGGTCCAG CCTAAATCTT 
ACCAGCTCCA AGTTTGAGGA ATTCATGACA AGGCACCAGG TACATGAAAA AGAGGAATTC 
AAGGCTCTGA AAACATTAAA TATCTTCTAT CAAGCAGGCA CTTCCAAAGC TGGAAATCCT 
GTTTTTTATT ATGTCACACG AAGGTTCAAG ACTGGCCAGA TCAACGGGGA TTTGCTTATA 
TACCACGTCT TACTAACTTT AAAGCCATAT TATGCAAAGC CATATGAGAT TGTAATGGAC 
CTCACTCATG CCGGGCCTAG CAATCGCTTT AAGACAGACT TCCTCTCCAA GTGGTTTGTT 
GTTTTCCCGG GCTTTGCTTA CGAGAACGTC TCTTCGGTCT ACATCTACAA CTGTAATTCT 
TGGGTTCGGG AGTACACCAA ATACCATGAG AGACTGCTCA CGGGACTCAA AGGCAGCAAA 
AGACTCTTTT TCATAGACTC TCCCTCAAAA CTGGCTGAGC ATATCGATTA TGATCAGCAA 
AAACTGCCAG CCGCGACCTT GGCTCTGGAT GAAGACCTCA AGGTGTACAA CAGTGCTCTC 
AAGCTGGCTC ACAAGGACAC CAAGGTGACC CTCAAGGTTG GCTCTACTGC GATCCAAGTG 
ACGTCATCTG AGCGAACTAA AGTCCTGGGA CAGACGGTTT TTCTGAATGA TATTTACTAT 
GCCTCTGAGA TCGAAGAAAT CTGTCTTGTG GATGAAAACC AGTTCACTCT GACCATTTCC 
AATCAGGGCA CTCCCCTCAC CTTCATGCAT CAAGAGTGTG AAGGCATTGT GGCGTCCATC 
ATTCACATCC GAACTCGATG GGAGCTGTCC CAGCCAGATT CTATCCCCCA GCACACTAAG 
ATCCGGCCAA AGGACGTTCC CGGAACACTT TTGAATATCG CATTACTTAA CTTGGGTAGT 
TCGGACCCAA GTTTACGGTC AGCCGCCTAT AATCTTCTCT GTGCCTTAAC CTGTACCTTT 
AATTTAAAAA TTGAGGGCCA ATTACTAGAA ACATCAGGTC TGTGTATACC TGCCAACAAT 
ACCCTTTTTA TTGTCTCTAT TAGTAAAACC CTGGCAGCTA ATGAGCCACA CCTCACCTTA 
GAGTTTTTGG AAGAGTGCAT TTCCGGATTT AGCAAATCCA GTATTGAACT GAAGCACCTG 
TGTCTAGAAT ACATGACTCC CTGGCTATCA AATCTAGTCC GTTTCTGTAA ACATAATGAT 
GATGCCAAAC GACAGAGAGT CACTGCTATT CTTGATAAAC TTATAACAAT GACCATAAAT 
GAAAAGCAGA TGTACCCATC GATCCAAGCC AAGATATGGG GAAGCCTTGG GCAGATTACA 
GATCTGCTCG ATGTAGTGCT GGACAGCTTT ATCAAAACCA GTGCCACAGG TGGTTTAGGA 
TCCATCAAGG CTGAAGTGAT GGCTGACACG GCCGTGGCTC TGGCTTCTGG GAACGTCAAA 
CTAGTTTCGA GCAAGGTGAT TGGAAGAATG TGCAAAATCA TTGACAAGAC CTGCCTCTCT 
CCAACTCCTA CCTTAGAGCA GCACCTCATG TGGGATGATA TCGCCATTTT AGCACGCTAC 
ATGCTAATGC TGTCCTTCAA TAATTCTCTG GATGTGGCGG CTCATCTTCC CTACCTCTTC 
CATGTGGTGA CTCTGTTGGT GGCCACAGGT CCCTTGTCCC TTCGTGCTTC CACACACGGA 
CTGGTCATCA ATATCATCCA TTCTCTGTGC ACGTGCTCAC AGCTTCACTT TAGTGAAGAG 
ACCAAGCAAG TTTTACGACT GAGTCTGACT GAGTTCTCAC TACCTAAGTT TTATTTGCTG 
TTCGGCATCA GCAAAGTCAA ATCAGCTGCC GTGATAGCCT TCCGGTCCAG TTACCGGGAC 
AGGTCTTTCT CCCCTGGCTC CTATGAGAGG GAGACCTTTG CCCTGGCCTC CTTGGAAACA 
GTCACAGAAG CTCTCTTGGA GATCATGGAG GCTTGTATGA GAGATATTCC TGCATGCAAG 
TGGCTGGACC AGTGGACAGA GCTAGCCCAG AGGTTTGCGT TCCAGTACAA TCCCTCCCTG 
CAGCCACGAG CTCTGGTTGT GTTTGGTTGT ATCAGCAAAA GAGTGTCCCA TGGGCAGATC 
AAGCAGATTA TCCGCATTCT TAGCAAGGGA CTTGAGAGCT GCCTAAAAGG ACCTGATAAT 
TATAACAGTC AAGTTCTGAT TGAAGCTACC GTGATAGCTC TAACCAAATT ACAACCACTT 
CTTAATAAGC AGGAGTCCCC TCTGCACCGA GCTCTCTTCT GGGTGGCCGT TGCCGTGCTG 
CAGCTGGATG AAGTGGACCT GTACTCGGCG GGCACTGCCC TCCTCGAACA GAACCTGCAC 
ACCTTAGACA GCCTCCTGCA CATCTTCAAC GACCAGAGCC CAGAAGAAGT GTTCATGGAG 
ATCCGGCAGC CTCTGGAGTG GCACTGCAAG CAGATGGACC ACTTTGTGGG GCTCAACTTT 
AACTCCAGCT TTAACTTTGC CCTGGTCGGG CACCTGCTGA AGGGATTTCG GCATCCTTCA 
CCTGTCACTG TTGCAAGAAC TGTGAGGATT TTGCACACCC TACTAGCATT GGTTAACAAG 
CATAGGAATT GTGACAAGTT TGAGGTGAAC ACCCAGAGTG TGGCTTACTT AGCAGCTTTA 
CTTACTGTGT CTGAGGAGGT TCGAAGTCGC TGCAGCTTAA AGCATAGGAA ATCACTCCTA 
CTAACTGATA TCACAATGGA AAATGTGCCT ATGGATATAT ATCCCATCTA TCACAGTGAC 
CCCAGCCTTA GGACACTAAA AGAAACACAA CCGTGGTCCT CTCCAAAGGG ATCTGAGAGA 
CATCTTGCAG CCAGCTACCC TCCTGTGGGC CAAACTAGCC CCCGAACCAG AAAATCCATG 
AGCCTGGATA TGGGGCAGCC TTCCCAGGCC AACACAAAGA AGTTGCTTGG GACAAGGAAA 
AGCTTCGATC ATTTGATCTC TGATGCCAAA GCTCCCAAAA GGCAAGAACA GGAGTCTGGA 
GTCACCACGC CCCCCAAGAT GCGGAGAGTG GCCGAGAACG ACTACGAGAT GGAAACCCAG 
AGAATCTCCC CTCAGCCACA CCCGCCTCTG CGGAAAGTGT CCGTGTCCGA ATCCAATGTG 
CTGCTGGACG AGGAAGTGCT CACAGACCCC AAAACCCAGG CTCTTCTTCT CACTGTCCTG 
GCTACTCTGG TGAAGTACAC TGCGGACGAG TTTGACCAGC GCATTCTCTA TGAGTACTTA 
GCGGAGGCCA GTGTTGTCTT CCCAAAGGTT TTTCCAGTGG TACATAATTT GTTGGACTCA 
AAGATAAATA CTCTGCTCTC ATTATGCCAA GACCCAAATT TGCTAAACCC GGTTCATGGA 
ATTGTGCAGA GTGTGGTCTA CCACGAAGAG GCCCCGCCGC AGTACCAGAC GGCGTACCTC 
CAGAGTTTCG GTTTTAATGG TCTGTGGAGG TTTGCAGGAC CATTTTCTAA GCAGACGCAG 
ATCCCAGACT ATGCCGAGCT CATCGTGAAG TTTCTCGATG CCTTGATTGA CACGTATCTG 
CCTGGAATTG ATGAGGAAGC CAGTGAGGAG TCCCTCCTGA CACCCACCTC TCCCTACCCG 
CCCGCGGTGC AGAGCCAGCT TAGCATCACT GCGAACCTCA ACCTCTCTAA CTCCATGACC 
TCACTGGCAA CTTCCCAGCA TTCCCCAGGC ATCGACAAGG AGAATGTGGA GCTCTCGCCA 
ACCACCGGGC ACTGCAGCGG CGGGAGGACG CGCCACGGCT CCGCCAGCCA GGTCCAGAAG 
CAAAGGAGTG CCGGCAGCTT CAAGAGGCAC AGCATCAAGA AGATCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967649.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823509.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X10, mRNA.

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CloneID	OSa234697
Clone ID Related Accession (Same CDS sequence)	XM_031967649.1
Accession Version	XM_031967649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGCAGTCA 
GATAGGAGTT CTTCCCATTT CCTCTACCTT TATGGGGCAG GCTATGATGT CTCTTCTGGT 
GGAGGTACCA GTCATATCTC TCTAGACCAC GAGGAGTTTA TGCGTACCTG TGCTCATGGA 
CCATCTCTCA GGAAAGTGAA AGGAAGCTCC TCAATGGACG GTGCAATCGG GTCCAGCGGG 
ACGCCCATCT GCCGCCAGGC CCAGACTAAG CTGGAGGTTG CTCTGTACAT GTTTCTGTGG 
AGTCCCGACA TTGAAGCCGT GCTCGTGGCC ATGTCCTGCT TTCGACACCT GTGTGAGGAA 
GCCGACATCA GGTGTGGCAT AGATGAGATC TCGGTGCACC ATTTCTTGCC AAACTATAAC 
ACCTTCATGG AGTTCGCATC TGTCAGCAAC ATGATGTCGA CAGGAAGAGC AGCTCTTCAG 
AAAAGAGTGA TGGCCCTGCT CAGGCGCATC GAACACCCCA CCGCTGGAAA CACCGAGGCT 
TGGGAAGATA CTTACGCCAA ATGGGAGCAA GCTACAAAAT CAATCCTCAA TTATCCGAAA 
GCCAAAATTG AAGATGGCCA GGTTGCTGAG AGTCTTCACA AGACCATTGT GAAGAGAAGA 
ATGTCTCACG TGAGTGGAGG GGGCTCCATA GACTTGTGCG ACACTGACTC GCTACAGGAG 
TGGATCAACA TGACTGGGTT TCTCTGTGCC CTTGGTGGCG TGTGCCTACA GCAGCGGAGC 
AATGCCGGCT TTGCCACCTA CAGCCCTCCC ATGGGCCCTA TCAGTGAACG CAAGGGCTCT 
CTCATTTCCA TGGTGTCCAG CGATGGAAAC GCAGAGACGC CTGTCAGCAA GTTCATGGAT 
CGGCTGCTCT CCTTGATGGT GTGTAACCAC GAAAAAGTGG GGCTGCAGAT ACGAAGCAAT 
GTCAAGGACT TGTTGGGTCT TGAATTGAGT CCTGCCCTTT ATCCAATGCT GTTTAACAAA 
TTAAAAAATA CCATCAGCAA GTTTTTTGAT CCTCAAGGAC AGGTTTTATT GACTGACACC 
AACACCCAGT TTGTGGAGCA GACCATAGCC ATAATGAAGA ACTTGCTTGA TAATCATGCT 
GAGGGTAGTT CTGAACACAT GGGACAAGCT AGCATCGAAA CTATGATGTT AAATCTGGTC 
AGATATGTTA GAGTGTTTGG GAATTTGGTG CAGGCAATTC AGATCAAAAC TAAGCTGTGC 
CAGCTGGTTC AAGTAATGAT GGAGAGGAGA GATGACCTTT CATTTTGCCA AGAGATGAAA 
TTTAGGAATA AAATGGTAGA ATATCTGACA GATTGGGTCA TGGGAACATC TAACCAAGCT 
GCAGATGAAG ATGTGAAATG TCTAACTAGA GATTTGGACC AGGCAAGCAT GGAAGCAGTG 
GTGTCATTGC TCGCTGGGCT CCCTCTGCAG CCTGAGGAAG GCGATGGGGT GGAATTGATG 
GAAGCCAAAT CCCAGCTGTT CCTTAAGTAT TTCACCCTCT TTATGAACCT TCTTAATGAC 
TGCAGTGAGG TCGAAGAAGA AAATGTCCAG ATTGGGGGCA GGAAGCGCGG CATGTCGCGA 
AGGCTGGCGT CCCTGCGCCA CTGCACCGTG CTGGCAATGT CCAACCTGCT CAATGCGAAT 
GTAGACAGCG GCCTGATGCA CTCTATAGTG CTAGGATTGG GTTACCACAA AGATCTTCAG 
ACAAGAGCCA CCTTCATGGA AGTCCTCACA AAAATCCTTC AGCAAGGCAC AGAGTTTGAT 
ACGCTCGCAG AAACCGTGTT AGCTGATCGA TTTGAGAGAC TTGTTGAGTT GGTCACGATG 
ATGGGTGATC AAGGAGAGCT CCCCATAGCC ATGGCTCTGG CCAACGTGGT GCCTTGTTCT 
CAGTGGGATG AACTTGCTCG AGTCCTTGTT ACTCTCTTTG ATTCCCGACA CTTACTGTAT 
CAGCTCCTTT GGAACATGTT TTCTAAGGAA GTTGAGCTGG CAGACTCCAT GCAGACACTT 
TTTAGAGGAA ACAGCTTGGC CAGTAAAATC ATGACCTTCT GCTTTAAGGT CTACGGTGCT 
ACATACCTGC AGAAGCTTCT GGAGCCTTTA CTCCGCTTTG TGATCACATC CTCCGAATGG 
CAGCACGTCA GCTTCGAGGT CGATCCCACC AGGTTAGAGC ATACTGAGAG CCTCGAGGAC 
AACCAGCGGA ACCTTTTGCA AATGACTGAG AAGTTCTTCC ACGCCATCAT CAGTTCTTCC 
TCCGAGTTCC CCCCTCAGCT ACGCAGTGTG TGCCACTGCC TGTACCAGGT TGTTAGCCAG 
AGATTCCCTC AGAACAGCAT TGGCGCCGTG GGGAGTGCCA TGTTCCTGAG ATTCATCAAC 
CCTGCCGTTG TCTCTCCCTA CGAGGCAGGG ATTTTAGAGA GAAAACCGCT CCCTAGAATT 
GAAAGGGGGT TGAAGCTGAT GTCAAAGATT CTTCAGAGCA TTGCCAATCA TGTCCTTTTC 
ACAAAAGAAG AGTATATGAG GCCTTTTAAC GATTTTGTGA AAAGCAACTT TGATGTAGCA 
CGGAGATTTT TCCTTGATAT CGCATCTGAT TGTCCTGTAA GTGATGCCAT AAACCACAGC 
CTTTCCTTCA TCAGCGATGG CAATGTCTTG GCTTTGCACC GTCTGCTCTG GAACAACCAA 
GAGAAAATCG GCCAATATCT TTCAAGCAAC AGAGATCACA AAGCTGTTGG AAGGCGACCT 
TTCGATAAGA TGGCAACACT GCTTGCCTAC CTGGGACCTC CAGAGCACAA GCCTGTGGCT 
GACACACACT GGTCCAGCCT AAATCTTACC AGCTCCAAGT TTGAGGAATT CATGACAAGG 
CACCAGGTAC ATGAAAAAGA GGAATTCAAG GCTCTGAAAA CATTAAATAT CTTCTATCAA 
GCAGGCACTT CCAAAGCTGG AAATCCTGTT TTTTATTATG TCACACGAAG GTTCAAGACT 
GGCCAGATCA ACGGGGATTT GCTTATATAC CACGTCTTAC TAACTTTAAA GCCATATTAT 
GCAAAGCCAT ATGAGATTGT AATGGACCTC ACTCATGCCG GGCCTAGCAA TCGCTTTAAG 
ACAGACTTCC TCTCCAAGTG GTTTGTTGTT TTCCCGGGCT TTGCTTACGA GAACGTCTCT 
TCGGTCTACA TCTACAACTG TAATTCTTGG GTTCGGGAGT ACACCAAATA CCATGAGAGA 
CTGCTCACGG GACTCAAAGG CAGCAAAAGA CTCTTTTTCA TAGACTCTCC CTCAAAACTG 
GCTGAGCATA TCGATTATGA TCAGCAAAAA CTGCCAGCCG CGACCTTGGC TCTGGATGAA 
GACCTCAAGG TGTACAACAG TGCTCTCAAG CTGGCTCACA AGGACACCAA GGTGACCCTC 
AAGGTTGGCT CTACTGCGAT CCAAGTGACG TCATCTGAGC GAACTAAAGT CCTGGGACAG 
ACGGTTTTTC TGAATGATAT TTACTATGCC TCTGAGATCG AAGAAATCTG TCTTGTGGAT 
GAAAACCAGT TCACTCTGAC CATTTCCAAT CAGGGCACTC CCCTCACCTT CATGCATCAA 
GAGTGTGAAG GCATTGTGGC GTCCATCATT CACATCCGAA CTCGATGGGA GCTGTCCCAG 
CCAGATTCTA TCCCCCAGCA CACTAAGATC CGGCCAAAGG ACGTTCCCGG AACACTTTTG 
AATATCGCAT TACTTAACTT GGGTAGTTCG GACCCAAGTT TACGGTCAGC CGCCTATAAT 
CTTCTCTGTG CCTTAACCTG TACCTTTAAT TTAAAAATTG AGGGCCAATT ACTAGAAACA 
TCAGGTCTGT GTATACCTGC CAACAATACC CTTTTTATTG TCTCTATTAG TAAAACCCTG 
GCAGCTAATG AGCCACACCT CACCTTAGAG TTTTTGGAAG AGTGCATTTC CGGATTTAGC 
AAATCCAGTA TTGAACTGAA GCACCTGTGT CTAGAATACA TGACTCCCTG GCTATCAAAT 
CTAGTCCGTT TCTGTAAACA TAATGATGAT GCCAAACGAC AGAGAGTCAC TGCTATTCTT 
GATAAACTTA TAACAATGAC CATAAATGAA AAGCAGATGT ACCCATCGAT CCAAGCCAAG 
ATATGGGGAA GCCTTGGGCA GATTACAGAT CTGCTCGATG TAGTGCTGGA CAGCTTTATC 
AAAACCAGTG CCACAGGTGG TTTAGGATCC ATCAAGGCTG AAGTGATGGC TGACACGGCC 
GTGGCTCTGG CTTCTGGGAA CGTCAAACTA GTTTCGAGCA AGGTGATTGG AAGAATGTGC 
AAAATCATTG ACAAGACCTG CCTCTCTCCA ACTCCTACCT TAGAGCAGCA CCTCATGTGG 
GATGATATCG CCATTTTAGC ACGCTACATG CTAATGCTGT CCTTCAATAA TTCTCTGGAT 
GTGGCGGCTC ATCTTCCCTA CCTCTTCCAT GTGGTGACTC TGTTGGTGGC CACAGGTCCC 
TTGTCCCTTC GTGCTTCCAC ACACGGACTG GTCATCAATA TCATCCATTC TCTGTGCACG 
TGCTCACAGC TTCACTTTAG TGAAGAGACC AAGCAAGTTT TACGACTGAG TCTGACTGAG 
TTCTCACTAC CTAAGTTTTA TTTGCTGTTC GGCATCAGCA AAGTCAAATC AGCTGCCGTG 
ATAGCCTTCC GGTCCAGTTA CCGGGACAGG TCTTTCTCCC CTGGCTCCTA TGAGAGGGAG 
ACCTTTGCCC TGGCCTCCTT GGAAACAGTC ACAGAAGCTC TCTTGGAGAT CATGGAGGCT 
TGTATGAGAG ATATTCCTGC ATGCAAGTGG CTGGACCAGT GGACAGAGCT AGCCCAGAGG 
TTTGCGTTCC AGTACAATCC CTCCCTGCAG CCACGAGCTC TGGTTGTGTT TGGTTGTATC 
AGCAAAAGAG TGTCCCATGG GCAGATCAAG CAGATTATCC GCATTCTTAG CAAGGGACTT 
GAGAGCTGCC TAAAAGGACC TGATAATTAT AACAGTCAAG TTCTGATTGA AGCTACCGTG 
ATAGCTCTAA CCAAATTACA ACCACTTCTT AATAAGCAGG AGTCCCCTCT GCACCGAGCT 
CTCTTCTGGG TGGCCGTTGC CGTGCTGCAG CTGGATGAAG TGGACCTGTA CTCGGCGGGC 
ACTGCCCTCC TCGAACAGAA CCTGCACACC TTAGACAGCC TCCTGCACAT CTTCAACGAC 
CAGAGCCCAG AAGAAGTGTT CATGGAGATC CGGCAGCCTC TGGAGTGGCA CTGCAAGCAG 
ATGGACCACT TTGTGGGGCT CAACTTTAAC TCCAGCTTTA ACTTTGCCCT GGTCGGGCAC 
CTGCTGAAGG GATTTCGGCA TCCTTCACCT GTCACTGTTG CAAGAACTGT GAGGATTTTG 
CACACCCTAC TAGCATTGGT TAACAAGCAT AGGAATTGTG ACAAGTTTGA GGTGAACACC 
CAGAGTGTGG CTTACTTAGC AGCTTTACTT ACTGTGTCTG AGGAGGTTCG AAGTCGCTGC 
AGCTTAAAGC ATAGGAAATC ACTCCTACTA ACTGATATCA CAATGGAAAA TGTGCCTATG 
GATATATATC CCATCTATCA CAGTGACCCC AGCCTTAGGA CACTAAAAGA AACACAACCG 
TGGTCCTCTC CAAAGGGATC TGAGAGACAT CTTGCAGCCA GCTACCCTCC TGTGGGCCAA 
ACTAGCCCCC GAACCAGAAA ATCCATGAGC CTGGATATGG GGCAGCCTTC CCAGGCCAAC 
ACAAAGAAGT TGCTTGGGAC AAGGAAAAGC TTCGATCATT TGATCTCTGA TGCCAAAGCT 
CCCAAAAGGC AAGAACAGGA GTCTGGAGTC ACCACGCCCC CCAAGATGCG GAGAGTGGCC 
GAGAACGACT ACGAGATGGA AACCCAGAGA ATCTCCCCTC AGCCACACCC GCCTCTGCGG 
AAAGTGTCCG TGTCCGAATC CAATGTGCTG CTGGACGAGG AAGTGCTCAC AGACCCCAAA 
ACCCAGGCTC TTCTTCTCAC TGTCCTGGCT ACTCTGGTGA AGTACACTGC GGACGAGTTT 
GACCAGCGCA TTCTCTATGA GTACTTAGCG GAGGCCAGTG TTGTCTTCCC AAAGGTTTTT 
CCAGTGGTAC ATAATTTGTT GGACTCAAAG ATAAATACTC TGCTCTCATT ATGCCAAGAC 
CCAAATTTGC TAAACCCGGT TCATGGAATT GTGCAGAGTG TGGTCTACCA CGAAGAGGCC 
CCGCCGCAGT ACCAGACGGC GTACCTCCAG AGTTTCGGTT TTAATGGTCT GTGGAGGTTT 
GCAGGACCAT TTTCTAAGCA GACGCAGATC CCAGACTATG CCGAGCTCAT CGTGAAGTTT 
CTCGATGCCT TGATTGACAC GTATCTGCCT GGAATTGATG AGGAAGCCAG TGAGGAGTCC 
CTCCTGACAC CCACCTCTCC CTACCCGCCC GCGGTGCAGA GCCAGCTTAG CATCACTGCG 
AACCTCAACC TCTCTAACTC CATGACCTCA CTGGCAACTT CCCAGCATTC CCCAGGCATC 
GACAAGGAGA ATGTGGAGCT CTCGCCAACC ACCGGGCACT GCAGCGGCGG GAGGACGCGC 
CACGGCTCCG CCAGCCAGGT CCAGAAGCAA AGGAGTGCCG GCAGCTTCAA GAGGCACAGC 
ATCAAGAAGA TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823509.1
CDS	9..8606
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGVSDLQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKFSTLLTTITAWILSPWLCDTDVSIPLSCQITRPQSSFKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIVLGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKQESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967649.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967649.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGCAGTCA 
GATAGGAGTT CTTCCCATTT CCTCTACCTT TATGGGGCAG GCTATGATGT CTCTTCTGGT 
GGAGGTACCA GTCATATCTC TCTAGACCAC GAGGAGTTTA TGCGTACCTG TGCTCATGGA 
CCATCTCTCA GGAAAGTGAA AGGAAGCTCC TCAATGGACG GTGCAATCGG GTCCAGCGGG 
ACGCCCATCT GCCGCCAGGC CCAGACTAAG CTGGAGGTTG CTCTGTACAT GTTTCTGTGG 
AGTCCCGACA TTGAAGCCGT GCTCGTGGCC ATGTCCTGCT TTCGACACCT GTGTGAGGAA 
GCCGACATCA GGTGTGGCAT AGATGAGATC TCGGTGCACC ATTTCTTGCC AAACTATAAC 
ACCTTCATGG AGTTCGCATC TGTCAGCAAC ATGATGTCGA CAGGAAGAGC AGCTCTTCAG 
AAAAGAGTGA TGGCCCTGCT CAGGCGCATC GAACACCCCA CCGCTGGAAA CACCGAGGCT 
TGGGAAGATA CTTACGCCAA ATGGGAGCAA GCTACAAAAT CAATCCTCAA TTATCCGAAA 
GCCAAAATTG AAGATGGCCA GGTTGCTGAG AGTCTTCACA AGACCATTGT GAAGAGAAGA 
ATGTCTCACG TGAGTGGAGG GGGCTCCATA GACTTGTGCG ACACTGACTC GCTACAGGAG 
TGGATCAACA TGACTGGGTT TCTCTGTGCC CTTGGTGGCG TGTGCCTACA GCAGCGGAGC 
AATGCCGGCT TTGCCACCTA CAGCCCTCCC ATGGGCCCTA TCAGTGAACG CAAGGGCTCT 
CTCATTTCCA TGGTGTCCAG CGATGGAAAC GCAGAGACGC CTGTCAGCAA GTTCATGGAT 
CGGCTGCTCT CCTTGATGGT GTGTAACCAC GAAAAAGTGG GGCTGCAGAT ACGAAGCAAT 
GTCAAGGACT TGTTGGGTCT TGAATTGAGT CCTGCCCTTT ATCCAATGCT GTTTAACAAA 
TTAAAAAATA CCATCAGCAA GTTTTTTGAT CCTCAAGGAC AGGTTTTATT GACTGACACC 
AACACCCAGT TTGTGGAGCA GACCATAGCC ATAATGAAGA ACTTGCTTGA TAATCATGCT 
GAGGGTAGTT CTGAACACAT GGGACAAGCT AGCATCGAAA CTATGATGTT AAATCTGGTC 
AGATATGTTA GAGTGTTTGG GAATTTGGTG CAGGCAATTC AGATCAAAAC TAAGCTGTGC 
CAGCTGGTTC AAGTAATGAT GGAGAGGAGA GATGACCTTT CATTTTGCCA AGAGATGAAA 
TTTAGGAATA AAATGGTAGA ATATCTGACA GATTGGGTCA TGGGAACATC TAACCAAGCT 
GCAGATGAAG ATGTGAAATG TCTAACTAGA GATTTGGACC AGGCAAGCAT GGAAGCAGTG 
GTGTCATTGC TCGCTGGGCT CCCTCTGCAG CCTGAGGAAG GCGATGGGGT GGAATTGATG 
GAAGCCAAAT CCCAGCTGTT CCTTAAGTAT TTCACCCTCT TTATGAACCT TCTTAATGAC 
TGCAGTGAGG TCGAAGAAGA AAATGTCCAG ATTGGGGGCA GGAAGCGCGG CATGTCGCGA 
AGGCTGGCGT CCCTGCGCCA CTGCACCGTG CTGGCAATGT CCAACCTGCT CAATGCGAAT 
GTAGACAGCG GCCTGATGCA CTCTATAGTG CTAGGATTGG GTTACCACAA AGATCTTCAG 
ACAAGAGCCA CCTTCATGGA AGTCCTCACA AAAATCCTTC AGCAAGGCAC AGAGTTTGAT 
ACGCTCGCAG AAACCGTGTT AGCTGATCGA TTTGAGAGAC TTGTTGAGTT GGTCACGATG 
ATGGGTGATC AAGGAGAGCT CCCCATAGCC ATGGCTCTGG CCAACGTGGT GCCTTGTTCT 
CAGTGGGATG AACTTGCTCG AGTCCTTGTT ACTCTCTTTG ATTCCCGACA CTTACTGTAT 
CAGCTCCTTT GGAACATGTT TTCTAAGGAA GTTGAGCTGG CAGACTCCAT GCAGACACTT 
TTTAGAGGAA ACAGCTTGGC CAGTAAAATC ATGACCTTCT GCTTTAAGGT CTACGGTGCT 
ACATACCTGC AGAAGCTTCT GGAGCCTTTA CTCCGCTTTG TGATCACATC CTCCGAATGG 
CAGCACGTCA GCTTCGAGGT CGATCCCACC AGGTTAGAGC ATACTGAGAG CCTCGAGGAC 
AACCAGCGGA ACCTTTTGCA AATGACTGAG AAGTTCTTCC ACGCCATCAT CAGTTCTTCC 
TCCGAGTTCC CCCCTCAGCT ACGCAGTGTG TGCCACTGCC TGTACCAGGT TGTTAGCCAG 
AGATTCCCTC AGAACAGCAT TGGCGCCGTG GGGAGTGCCA TGTTCCTGAG ATTCATCAAC 
CCTGCCGTTG TCTCTCCCTA CGAGGCAGGG ATTTTAGAGA GAAAACCGCT CCCTAGAATT 
GAAAGGGGGT TGAAGCTGAT GTCAAAGATT CTTCAGAGCA TTGCCAATCA TGTCCTTTTC 
ACAAAAGAAG AGTATATGAG GCCTTTTAAC GATTTTGTGA AAAGCAACTT TGATGTAGCA 
CGGAGATTTT TCCTTGATAT CGCATCTGAT TGTCCTGTAA GTGATGCCAT AAACCACAGC 
CTTTCCTTCA TCAGCGATGG CAATGTCTTG GCTTTGCACC GTCTGCTCTG GAACAACCAA 
GAGAAAATCG GCCAATATCT TTCAAGCAAC AGAGATCACA AAGCTGTTGG AAGGCGACCT 
TTCGATAAGA TGGCAACACT GCTTGCCTAC CTGGGACCTC CAGAGCACAA GCCTGTGGCT 
GACACACACT GGTCCAGCCT AAATCTTACC AGCTCCAAGT TTGAGGAATT CATGACAAGG 
CACCAGGTAC ATGAAAAAGA GGAATTCAAG GCTCTGAAAA CATTAAATAT CTTCTATCAA 
GCAGGCACTT CCAAAGCTGG AAATCCTGTT TTTTATTATG TCACACGAAG GTTCAAGACT 
GGCCAGATCA ACGGGGATTT GCTTATATAC CACGTCTTAC TAACTTTAAA GCCATATTAT 
GCAAAGCCAT ATGAGATTGT AATGGACCTC ACTCATGCCG GGCCTAGCAA TCGCTTTAAG 
ACAGACTTCC TCTCCAAGTG GTTTGTTGTT TTCCCGGGCT TTGCTTACGA GAACGTCTCT 
TCGGTCTACA TCTACAACTG TAATTCTTGG GTTCGGGAGT ACACCAAATA CCATGAGAGA 
CTGCTCACGG GACTCAAAGG CAGCAAAAGA CTCTTTTTCA TAGACTCTCC CTCAAAACTG 
GCTGAGCATA TCGATTATGA TCAGCAAAAA CTGCCAGCCG CGACCTTGGC TCTGGATGAA 
GACCTCAAGG TGTACAACAG TGCTCTCAAG CTGGCTCACA AGGACACCAA GGTGACCCTC 
AAGGTTGGCT CTACTGCGAT CCAAGTGACG TCATCTGAGC GAACTAAAGT CCTGGGACAG 
ACGGTTTTTC TGAATGATAT TTACTATGCC TCTGAGATCG AAGAAATCTG TCTTGTGGAT 
GAAAACCAGT TCACTCTGAC CATTTCCAAT CAGGGCACTC CCCTCACCTT CATGCATCAA 
GAGTGTGAAG GCATTGTGGC GTCCATCATT CACATCCGAA CTCGATGGGA GCTGTCCCAG 
CCAGATTCTA TCCCCCAGCA CACTAAGATC CGGCCAAAGG ACGTTCCCGG AACACTTTTG 
AATATCGCAT TACTTAACTT GGGTAGTTCG GACCCAAGTT TACGGTCAGC CGCCTATAAT 
CTTCTCTGTG CCTTAACCTG TACCTTTAAT TTAAAAATTG AGGGCCAATT ACTAGAAACA 
TCAGGTCTGT GTATACCTGC CAACAATACC CTTTTTATTG TCTCTATTAG TAAAACCCTG 
GCAGCTAATG AGCCACACCT CACCTTAGAG TTTTTGGAAG AGTGCATTTC CGGATTTAGC 
AAATCCAGTA TTGAACTGAA GCACCTGTGT CTAGAATACA TGACTCCCTG GCTATCAAAT 
CTAGTCCGTT TCTGTAAACA TAATGATGAT GCCAAACGAC AGAGAGTCAC TGCTATTCTT 
GATAAACTTA TAACAATGAC CATAAATGAA AAGCAGATGT ACCCATCGAT CCAAGCCAAG 
ATATGGGGAA GCCTTGGGCA GATTACAGAT CTGCTCGATG TAGTGCTGGA CAGCTTTATC 
AAAACCAGTG CCACAGGTGG TTTAGGATCC ATCAAGGCTG AAGTGATGGC TGACACGGCC 
GTGGCTCTGG CTTCTGGGAA CGTCAAACTA GTTTCGAGCA AGGTGATTGG AAGAATGTGC 
AAAATCATTG ACAAGACCTG CCTCTCTCCA ACTCCTACCT TAGAGCAGCA CCTCATGTGG 
GATGATATCG CCATTTTAGC ACGCTACATG CTAATGCTGT CCTTCAATAA TTCTCTGGAT 
GTGGCGGCTC ATCTTCCCTA CCTCTTCCAT GTGGTGACTC TGTTGGTGGC CACAGGTCCC 
TTGTCCCTTC GTGCTTCCAC ACACGGACTG GTCATCAATA TCATCCATTC TCTGTGCACG 
TGCTCACAGC TTCACTTTAG TGAAGAGACC AAGCAAGTTT TACGACTGAG TCTGACTGAG 
TTCTCACTAC CTAAGTTTTA TTTGCTGTTC GGCATCAGCA AAGTCAAATC AGCTGCCGTG 
ATAGCCTTCC GGTCCAGTTA CCGGGACAGG TCTTTCTCCC CTGGCTCCTA TGAGAGGGAG 
ACCTTTGCCC TGGCCTCCTT GGAAACAGTC ACAGAAGCTC TCTTGGAGAT CATGGAGGCT 
TGTATGAGAG ATATTCCTGC ATGCAAGTGG CTGGACCAGT GGACAGAGCT AGCCCAGAGG 
TTTGCGTTCC AGTACAATCC CTCCCTGCAG CCACGAGCTC TGGTTGTGTT TGGTTGTATC 
AGCAAAAGAG TGTCCCATGG GCAGATCAAG CAGATTATCC GCATTCTTAG CAAGGGACTT 
GAGAGCTGCC TAAAAGGACC TGATAATTAT AACAGTCAAG TTCTGATTGA AGCTACCGTG 
ATAGCTCTAA CCAAATTACA ACCACTTCTT AATAAGCAGG AGTCCCCTCT GCACCGAGCT 
CTCTTCTGGG TGGCCGTTGC CGTGCTGCAG CTGGATGAAG TGGACCTGTA CTCGGCGGGC 
ACTGCCCTCC TCGAACAGAA CCTGCACACC TTAGACAGCC TCCTGCACAT CTTCAACGAC 
CAGAGCCCAG AAGAAGTGTT CATGGAGATC CGGCAGCCTC TGGAGTGGCA CTGCAAGCAG 
ATGGACCACT TTGTGGGGCT CAACTTTAAC TCCAGCTTTA ACTTTGCCCT GGTCGGGCAC 
CTGCTGAAGG GATTTCGGCA TCCTTCACCT GTCACTGTTG CAAGAACTGT GAGGATTTTG 
CACACCCTAC TAGCATTGGT TAACAAGCAT AGGAATTGTG ACAAGTTTGA GGTGAACACC 
CAGAGTGTGG CTTACTTAGC AGCTTTACTT ACTGTGTCTG AGGAGGTTCG AAGTCGCTGC 
AGCTTAAAGC ATAGGAAATC ACTCCTACTA ACTGATATCA CAATGGAAAA TGTGCCTATG 
GATATATATC CCATCTATCA CAGTGACCCC AGCCTTAGGA CACTAAAAGA AACACAACCG 
TGGTCCTCTC CAAAGGGATC TGAGAGACAT CTTGCAGCCA GCTACCCTCC TGTGGGCCAA 
ACTAGCCCCC GAACCAGAAA ATCCATGAGC CTGGATATGG GGCAGCCTTC CCAGGCCAAC 
ACAAAGAAGT TGCTTGGGAC AAGGAAAAGC TTCGATCATT TGATCTCTGA TGCCAAAGCT 
CCCAAAAGGC AAGAACAGGA GTCTGGAGTC ACCACGCCCC CCAAGATGCG GAGAGTGGCC 
GAGAACGACT ACGAGATGGA AACCCAGAGA ATCTCCCCTC AGCCACACCC GCCTCTGCGG 
AAAGTGTCCG TGTCCGAATC CAATGTGCTG CTGGACGAGG AAGTGCTCAC AGACCCCAAA 
ACCCAGGCTC TTCTTCTCAC TGTCCTGGCT ACTCTGGTGA AGTACACTGC GGACGAGTTT 
GACCAGCGCA TTCTCTATGA GTACTTAGCG GAGGCCAGTG TTGTCTTCCC AAAGGTTTTT 
CCAGTGGTAC ATAATTTGTT GGACTCAAAG ATAAATACTC TGCTCTCATT ATGCCAAGAC 
CCAAATTTGC TAAACCCGGT TCATGGAATT GTGCAGAGTG TGGTCTACCA CGAAGAGGCC 
CCGCCGCAGT ACCAGACGGC GTACCTCCAG AGTTTCGGTT TTAATGGTCT GTGGAGGTTT 
GCAGGACCAT TTTCTAAGCA GACGCAGATC CCAGACTATG CCGAGCTCAT CGTGAAGTTT 
CTCGATGCCT TGATTGACAC GTATCTGCCT GGAATTGATG AGGAAGCCAG TGAGGAGTCC 
CTCCTGACAC CCACCTCTCC CTACCCGCCC GCGGTGCAGA GCCAGCTTAG CATCACTGCG 
AACCTCAACC TCTCTAACTC CATGACCTCA CTGGCAACTT CCCAGCATTC CCCAGGCATC 
GACAAGGAGA ATGTGGAGCT CTCGCCAACC ACCGGGCACT GCAGCGGCGG GAGGACGCGC 
CACGGCTCCG CCAGCCAGGT CCAGAAGCAA AGGAGTGCCG GCAGCTTCAA GAGGCACAGC 
ATCAAGAAGA TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967650.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823510.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X11, mRNA.

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CloneID	OSa234698
Clone ID Related Accession (Same CDS sequence)	XM_031967650.1
Accession Version	XM_031967650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGCAGTCA 
GATAGGAGTT CTTCCCATTT CCTCTACCTT TATGGGGCAG GCTATGATGT CTCTTCTGGT 
GGAGGTACCA GTCATATCTC TCTAGACCAC GAGGAGTTTA TGCGTACCTG TGCTCATGGA 
CCATCTCTCA GGAAAGTGAA AGGAAGCTCC TCAATGGACG GTGCAATCGG GTCCAGCGGG 
ACGCCCATCT GCCGCCAGGC CCAGACTAAG CTGGAGGTTG CTCTGTACAT GTTTCTGTGG 
AGTCCCGACA TTGAAGCCGT GCTCGTGGCC ATGTCCTGCT TTCGACACCT GTGTGAGGAA 
GCCGACATCA GGTGTGGCAT AGATGAGATC TCGGTGCACC ATTTCTTGCC AAACTATAAC 
ACCTTCATGG AGTTCGCATC TGTCAGCAAC ATGATGTCGA CAGGAAGAGC AGCTCTTCAG 
AAAAGAGTGA TGGCCCTGCT CAGGCGCATC GAACACCCCA CCGCTGGAAA CACCGAGGCT 
TGGGAAGATA CTTACGCCAA ATGGGAGCAA GCTACAAAAT CAATCCTCAA TTATCCGAAA 
GCCAAAATTG AAGATGGCCA GGTTGCTGAG AGTCTTCACA AGACCATTGT GAAGAGAAGA 
ATGTCTCACG TGAGTGGAGG GGGCTCCATA GACTTGTGCG ACACTGACTC GCTACAGGAG 
TGGATCAACA TGACTGGGTT TCTCTGTGCC CTTGGTGGCG TGTGCCTACA GCAGCGGAGC 
AATGCCGGCT TTGCCACCTA CAGCCCTCCC ATGGGCCCTA TCAGTGAACG CAAGGGCTCT 
CTCATTTCCA TGGTGTCCAG CGATGGAAAC GCAGAGACGC CTGTCAGCAA GTTCATGGAT 
CGGCTGCTCT CCTTGATGGT GTGTAACCAC GAAAAAGTGG GGCTGCAGAT ACGAAGCAAT 
GTCAAGGACT TGTTGGGTCT TGAATTGAGT CCTGCCCTTT ATCCAATGCT GTTTAACAAA 
TTAAAAAATA CCATCAGCAA GTTTTTTGAT CCTCAAGGAC AGGTTTTATT GACTGACACC 
AACACCCAGT TTGTGGAGCA GACCATAGCC ATAATGAAGA ACTTGCTTGA TAATCATGCT 
GAGGGTAGTT CTGAACACAT GGGACAAGCT AGCATCGAAA CTATGATGTT AAATCTGGTC 
AGATATGTTA GAGTGTTTGG GAATTTGGTG CAGGCAATTC AGATCAAAAC TAAGCTGTGC 
CAGCTGGTTC AAGTAATGAT GGAGAGGAGA GATGACCTTT CATTTTGCCA AGAGATGAAA 
TTTAGGAATA AAATGGTAGA ATATCTGACA GATTGGGTCA TGGGAACATC TAACCAAGCT 
GCAGATGAAG ATGTGAAATG TCTAACTAGA GATTTGGACC AGGCAAGCAT GGAAGCAGTG 
GTGTCATTGC TCGCTGGGCT CCCTCTGCAG CCTGAGGAAG GCGATGGGGT GGAATTGATG 
GAAGCCAAAT CCCAGCTGTT CCTTAAGTAT TTCACCCTCT TTATGAACCT TCTTAATGAC 
TGCAGTGAGG TCGAAGAAGA AAATGTCCAG ATTGGGGGCA GGAAGCGCGG CATGTCGCGA 
AGGCTGGCGT CCCTGCGCCA CTGCACCGTG CTGGCAATGT CCAACCTGCT CAATGCGAAT 
GTAGACAGCG GCCTGATGCA CTCTATAGGA TTGGGTTACC ACAAAGATCT TCAGACAAGA 
GCCACCTTCA TGGAAGTCCT CACAAAAATC CTTCAGCAAG GCACAGAGTT TGATACGCTC 
GCAGAAACCG TGTTAGCTGA TCGATTTGAG AGACTTGTTG AGTTGGTCAC GATGATGGGT 
GATCAAGGAG AGCTCCCCAT AGCCATGGCT CTGGCCAACG TGGTGCCTTG TTCTCAGTGG 
GATGAACTTG CTCGAGTCCT TGTTACTCTC TTTGATTCCC GACACTTACT GTATCAGCTC 
CTTTGGAACA TGTTTTCTAA GGAAGTTGAG CTGGCAGACT CCATGCAGAC ACTTTTTAGA 
GGAAACAGCT TGGCCAGTAA AATCATGACC TTCTGCTTTA AGGTCTACGG TGCTACATAC 
CTGCAGAAGC TTCTGGAGCC TTTACTCCGC TTTGTGATCA CATCCTCCGA ATGGCAGCAC 
GTCAGCTTCG AGGTCGATCC CACCAGGTTA GAGCATACTG AGAGCCTCGA GGACAACCAG 
CGGAACCTTT TGCAAATGAC TGAGAAGTTC TTCCACGCCA TCATCAGTTC TTCCTCCGAG 
TTCCCCCCTC AGCTACGCAG TGTGTGCCAC TGCCTGTACC AGGTTGTTAG CCAGAGATTC 
CCTCAGAACA GCATTGGCGC CGTGGGGAGT GCCATGTTCC TGAGATTCAT CAACCCTGCC 
GTTGTCTCTC CCTACGAGGC AGGGATTTTA GAGAGAAAAC CGCTCCCTAG AATTGAAAGG 
GGGTTGAAGC TGATGTCAAA GATTCTTCAG AGCATTGCCA ATCATGTCCT TTTCACAAAA 
GAAGAGTATA TGAGGCCTTT TAACGATTTT GTGAAAAGCA ACTTTGATGT AGCACGGAGA 
TTTTTCCTTG ATATCGCATC TGATTGTCCT GTAAGTGATG CCATAAACCA CAGCCTTTCC 
TTCATCAGCG ATGGCAATGT CTTGGCTTTG CACCGTCTGC TCTGGAACAA CCAAGAGAAA 
ATCGGCCAAT ATCTTTCAAG CAACAGAGAT CACAAAGCTG TTGGAAGGCG ACCTTTCGAT 
AAGATGGCAA CACTGCTTGC CTACCTGGGA CCTCCAGAGC ACAAGCCTGT GGCTGACACA 
CACTGGTCCA GCCTAAATCT TACCAGCTCC AAGTTTGAGG AATTCATGAC AAGGCACCAG 
GTACATGAAA AAGAGGAATT CAAGGCTCTG AAAACATTAA ATATCTTCTA TCAAGCAGGC 
ACTTCCAAAG CTGGAAATCC TGTTTTTTAT TATGTCACAC GAAGGTTCAA GACTGGCCAG 
ATCAACGGGG ATTTGCTTAT ATACCACGTC TTACTAACTT TAAAGCCATA TTATGCAAAG 
CCATATGAGA TTGTAATGGA CCTCACTCAT GCCGGGCCTA GCAATCGCTT TAAGACAGAC 
TTCCTCTCCA AGTGGTTTGT TGTTTTCCCG GGCTTTGCTT ACGAGAACGT CTCTTCGGTC 
TACATCTACA ACTGTAATTC TTGGGTTCGG GAGTACACCA AATACCATGA GAGACTGCTC 
ACGGGACTCA AAGGCAGCAA AAGACTCTTT TTCATAGACT CTCCCTCAAA ACTGGCTGAG 
CATATCGATT ATGATCAGCA AAAACTGCCA GCCGCGACCT TGGCTCTGGA TGAAGACCTC 
AAGGTGTACA ACAGTGCTCT CAAGCTGGCT CACAAGGACA CCAAGGTGAC CCTCAAGGTT 
GGCTCTACTG CGATCCAAGT GACGTCATCT GAGCGAACTA AAGTCCTGGG ACAGACGGTT 
TTTCTGAATG ATATTTACTA TGCCTCTGAG ATCGAAGAAA TCTGTCTTGT GGATGAAAAC 
CAGTTCACTC TGACCATTTC CAATCAGGGC ACTCCCCTCA CCTTCATGCA TCAAGAGTGT 
GAAGGCATTG TGGCGTCCAT CATTCACATC CGAACTCGAT GGGAGCTGTC CCAGCCAGAT 
TCTATCCCCC AGCACACTAA GATCCGGCCA AAGGACGTTC CCGGAACACT TTTGAATATC 
GCATTACTTA ACTTGGGTAG TTCGGACCCA AGTTTACGGT CAGCCGCCTA TAATCTTCTC 
TGTGCCTTAA CCTGTACCTT TAATTTAAAA ATTGAGGGCC AATTACTAGA AACATCAGGT 
CTGTGTATAC CTGCCAACAA TACCCTTTTT ATTGTCTCTA TTAGTAAAAC CCTGGCAGCT 
AATGAGCCAC ACCTCACCTT AGAGTTTTTG GAAGAGTGCA TTTCCGGATT TAGCAAATCC 
AGTATTGAAC TGAAGCACCT GTGTCTAGAA TACATGACTC CCTGGCTATC AAATCTAGTC 
CGTTTCTGTA AACATAATGA TGATGCCAAA CGACAGAGAG TCACTGCTAT TCTTGATAAA 
CTTATAACAA TGACCATAAA TGAAAAGCAG ATGTACCCAT CGATCCAAGC CAAGATATGG 
GGAAGCCTTG GGCAGATTAC AGATCTGCTC GATGTAGTGC TGGACAGCTT TATCAAAACC 
AGTGCCACAG GTGGTTTAGG ATCCATCAAG GCTGAAGTGA TGGCTGACAC GGCCGTGGCT 
CTGGCTTCTG GGAACGTCAA ACTAGTTTCG AGCAAGGTGA TTGGAAGAAT GTGCAAAATC 
ATTGACAAGA CCTGCCTCTC TCCAACTCCT ACCTTAGAGC AGCACCTCAT GTGGGATGAT 
ATCGCCATTT TAGCACGCTA CATGCTAATG CTGTCCTTCA ATAATTCTCT GGATGTGGCG 
GCTCATCTTC CCTACCTCTT CCATGTGGTG ACTCTGTTGG TGGCCACAGG TCCCTTGTCC 
CTTCGTGCTT CCACACACGG ACTGGTCATC AATATCATCC ATTCTCTGTG CACGTGCTCA 
CAGCTTCACT TTAGTGAAGA GACCAAGCAA GTTTTACGAC TGAGTCTGAC TGAGTTCTCA 
CTACCTAAGT TTTATTTGCT GTTCGGCATC AGCAAAGTCA AATCAGCTGC CGTGATAGCC 
TTCCGGTCCA GTTACCGGGA CAGGTCTTTC TCCCCTGGCT CCTATGAGAG GGAGACCTTT 
GCCCTGGCCT CCTTGGAAAC AGTCACAGAA GCTCTCTTGG AGATCATGGA GGCTTGTATG 
AGAGATATTC CTGCATGCAA GTGGCTGGAC CAGTGGACAG AGCTAGCCCA GAGGTTTGCG 
TTCCAGTACA ATCCCTCCCT GCAGCCACGA GCTCTGGTTG TGTTTGGTTG TATCAGCAAA 
AGAGTGTCCC ATGGGCAGAT CAAGCAGATT ATCCGCATTC TTAGCAAGGG ACTTGAGAGC 
TGCCTAAAAG GACCTGATAA TTATAACAGT CAAGTTCTGA TTGAAGCTAC CGTGATAGCT 
CTAACCAAAT TACAACCACT TCTTAATAAG CAGGAGTCCC CTCTGCACCG AGCTCTCTTC 
TGGGTGGCCG TTGCCGTGCT GCAGCTGGAT GAAGTGGACC TGTACTCGGC GGGCACTGCC 
CTCCTCGAAC AGAACCTGCA CACCTTAGAC AGCCTCCTGC ACATCTTCAA CGACCAGAGC 
CCAGAAGAAG TGTTCATGGA GATCCGGCAG CCTCTGGAGT GGCACTGCAA GCAGATGGAC 
CACTTTGTGG GGCTCAACTT TAACTCCAGC TTTAACTTTG CCCTGGTCGG GCACCTGCTG 
AAGGGATTTC GGCATCCTTC ACCTGTCACT GTTGCAAGAA CTGTGAGGAT TTTGCACACC 
CTACTAGCAT TGGTTAACAA GCATAGGAAT TGTGACAAGT TTGAGGTGAA CACCCAGAGT 
GTGGCTTACT TAGCAGCTTT ACTTACTGTG TCTGAGGAGG TTCGAAGTCG CTGCAGCTTA 
AAGCATAGGA AATCACTCCT ACTAACTGAT ATCACAATGG AAAATGTGCC TATGGATATA 
TATCCCATCT ATCACAGTGA CCCCAGCCTT AGGACACTAA AAGAAACACA ACCGTGGTCC 
TCTCCAAAGG GATCTGAGAG ACATCTTGCA GCCAGCTACC CTCCTGTGGG CCAAACTAGC 
CCCCGAACCA GAAAATCCAT GAGCCTGGAT ATGGGGCAGC CTTCCCAGGC CAACACAAAG 
AAGTTGCTTG GGACAAGGAA AAGCTTCGAT CATTTGATCT CTGATGCCAA AGCTCCCAAA 
AGGCAAGAAC AGGAGTCTGG AGTCACCACG CCCCCCAAGA TGCGGAGAGT GGCCGAGAAC 
GACTACGAGA TGGAAACCCA GAGAATCTCC CCTCAGCCAC ACCCGCCTCT GCGGAAAGTG 
TCCGTGTCCG AATCCAATGT GCTGCTGGAC GAGGAAGTGC TCACAGACCC CAAAACCCAG 
GCTCTTCTTC TCACTGTCCT GGCTACTCTG GTGAAGTACA CTGCGGACGA GTTTGACCAG 
CGCATTCTCT ATGAGTACTT AGCGGAGGCC AGTGTTGTCT TCCCAAAGGT TTTTCCAGTG 
GTACATAATT TGTTGGACTC AAAGATAAAT ACTCTGCTCT CATTATGCCA AGACCCAAAT 
TTGCTAAACC CGGTTCATGG AATTGTGCAG AGTGTGGTCT ACCACGAAGA GGCCCCGCCG 
CAGTACCAGA CGGCGTACCT CCAGAGTTTC GGTTTTAATG GTCTGTGGAG GTTTGCAGGA 
CCATTTTCTA AGCAGACGCA GATCCCAGAC TATGCCGAGC TCATCGTGAA GTTTCTCGAT 
GCCTTGATTG ACACGTATCT GCCTGGAATT GATGAGGAAG CCAGTGAGGA GTCCCTCCTG 
ACACCCACCT CTCCCTACCC GCCCGCGGTG CAGAGCCAGC TTAGCATCAC TGCGAACCTC 
AACCTCTCTA ACTCCATGAC CTCACTGGCA ACTTCCCAGC ATTCCCCAGG CATCGACAAG 
GAGAATGTGG AGCTCTCGCC AACCACCGGG CACTGCAGCG GCGGGAGGAC GCGCCACGGC 
TCCGCCAGCC AGGTCCAGAA GCAAAGGAGT GCCGGCAGCT TCAAGAGGCA CAGCATCAAG 
AAGATCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823510.1
CDS	9..8600
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGVSDLQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKFSTLLTTITAWILSPWLCDTDVSIPLSCQITRPQSSFKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKQESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967650.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967650.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGCAGTCA 
GATAGGAGTT CTTCCCATTT CCTCTACCTT TATGGGGCAG GCTATGATGT CTCTTCTGGT 
GGAGGTACCA GTCATATCTC TCTAGACCAC GAGGAGTTTA TGCGTACCTG TGCTCATGGA 
CCATCTCTCA GGAAAGTGAA AGGAAGCTCC TCAATGGACG GTGCAATCGG GTCCAGCGGG 
ACGCCCATCT GCCGCCAGGC CCAGACTAAG CTGGAGGTTG CTCTGTACAT GTTTCTGTGG 
AGTCCCGACA TTGAAGCCGT GCTCGTGGCC ATGTCCTGCT TTCGACACCT GTGTGAGGAA 
GCCGACATCA GGTGTGGCAT AGATGAGATC TCGGTGCACC ATTTCTTGCC AAACTATAAC 
ACCTTCATGG AGTTCGCATC TGTCAGCAAC ATGATGTCGA CAGGAAGAGC AGCTCTTCAG 
AAAAGAGTGA TGGCCCTGCT CAGGCGCATC GAACACCCCA CCGCTGGAAA CACCGAGGCT 
TGGGAAGATA CTTACGCCAA ATGGGAGCAA GCTACAAAAT CAATCCTCAA TTATCCGAAA 
GCCAAAATTG AAGATGGCCA GGTTGCTGAG AGTCTTCACA AGACCATTGT GAAGAGAAGA 
ATGTCTCACG TGAGTGGAGG GGGCTCCATA GACTTGTGCG ACACTGACTC GCTACAGGAG 
TGGATCAACA TGACTGGGTT TCTCTGTGCC CTTGGTGGCG TGTGCCTACA GCAGCGGAGC 
AATGCCGGCT TTGCCACCTA CAGCCCTCCC ATGGGCCCTA TCAGTGAACG CAAGGGCTCT 
CTCATTTCCA TGGTGTCCAG CGATGGAAAC GCAGAGACGC CTGTCAGCAA GTTCATGGAT 
CGGCTGCTCT CCTTGATGGT GTGTAACCAC GAAAAAGTGG GGCTGCAGAT ACGAAGCAAT 
GTCAAGGACT TGTTGGGTCT TGAATTGAGT CCTGCCCTTT ATCCAATGCT GTTTAACAAA 
TTAAAAAATA CCATCAGCAA GTTTTTTGAT CCTCAAGGAC AGGTTTTATT GACTGACACC 
AACACCCAGT TTGTGGAGCA GACCATAGCC ATAATGAAGA ACTTGCTTGA TAATCATGCT 
GAGGGTAGTT CTGAACACAT GGGACAAGCT AGCATCGAAA CTATGATGTT AAATCTGGTC 
AGATATGTTA GAGTGTTTGG GAATTTGGTG CAGGCAATTC AGATCAAAAC TAAGCTGTGC 
CAGCTGGTTC AAGTAATGAT GGAGAGGAGA GATGACCTTT CATTTTGCCA AGAGATGAAA 
TTTAGGAATA AAATGGTAGA ATATCTGACA GATTGGGTCA TGGGAACATC TAACCAAGCT 
GCAGATGAAG ATGTGAAATG TCTAACTAGA GATTTGGACC AGGCAAGCAT GGAAGCAGTG 
GTGTCATTGC TCGCTGGGCT CCCTCTGCAG CCTGAGGAAG GCGATGGGGT GGAATTGATG 
GAAGCCAAAT CCCAGCTGTT CCTTAAGTAT TTCACCCTCT TTATGAACCT TCTTAATGAC 
TGCAGTGAGG TCGAAGAAGA AAATGTCCAG ATTGGGGGCA GGAAGCGCGG CATGTCGCGA 
AGGCTGGCGT CCCTGCGCCA CTGCACCGTG CTGGCAATGT CCAACCTGCT CAATGCGAAT 
GTAGACAGCG GCCTGATGCA CTCTATAGGA TTGGGTTACC ACAAAGATCT TCAGACAAGA 
GCCACCTTCA TGGAAGTCCT CACAAAAATC CTTCAGCAAG GCACAGAGTT TGATACGCTC 
GCAGAAACCG TGTTAGCTGA TCGATTTGAG AGACTTGTTG AGTTGGTCAC GATGATGGGT 
GATCAAGGAG AGCTCCCCAT AGCCATGGCT CTGGCCAACG TGGTGCCTTG TTCTCAGTGG 
GATGAACTTG CTCGAGTCCT TGTTACTCTC TTTGATTCCC GACACTTACT GTATCAGCTC 
CTTTGGAACA TGTTTTCTAA GGAAGTTGAG CTGGCAGACT CCATGCAGAC ACTTTTTAGA 
GGAAACAGCT TGGCCAGTAA AATCATGACC TTCTGCTTTA AGGTCTACGG TGCTACATAC 
CTGCAGAAGC TTCTGGAGCC TTTACTCCGC TTTGTGATCA CATCCTCCGA ATGGCAGCAC 
GTCAGCTTCG AGGTCGATCC CACCAGGTTA GAGCATACTG AGAGCCTCGA GGACAACCAG 
CGGAACCTTT TGCAAATGAC TGAGAAGTTC TTCCACGCCA TCATCAGTTC TTCCTCCGAG 
TTCCCCCCTC AGCTACGCAG TGTGTGCCAC TGCCTGTACC AGGTTGTTAG CCAGAGATTC 
CCTCAGAACA GCATTGGCGC CGTGGGGAGT GCCATGTTCC TGAGATTCAT CAACCCTGCC 
GTTGTCTCTC CCTACGAGGC AGGGATTTTA GAGAGAAAAC CGCTCCCTAG AATTGAAAGG 
GGGTTGAAGC TGATGTCAAA GATTCTTCAG AGCATTGCCA ATCATGTCCT TTTCACAAAA 
GAAGAGTATA TGAGGCCTTT TAACGATTTT GTGAAAAGCA ACTTTGATGT AGCACGGAGA 
TTTTTCCTTG ATATCGCATC TGATTGTCCT GTAAGTGATG CCATAAACCA CAGCCTTTCC 
TTCATCAGCG ATGGCAATGT CTTGGCTTTG CACCGTCTGC TCTGGAACAA CCAAGAGAAA 
ATCGGCCAAT ATCTTTCAAG CAACAGAGAT CACAAAGCTG TTGGAAGGCG ACCTTTCGAT 
AAGATGGCAA CACTGCTTGC CTACCTGGGA CCTCCAGAGC ACAAGCCTGT GGCTGACACA 
CACTGGTCCA GCCTAAATCT TACCAGCTCC AAGTTTGAGG AATTCATGAC AAGGCACCAG 
GTACATGAAA AAGAGGAATT CAAGGCTCTG AAAACATTAA ATATCTTCTA TCAAGCAGGC 
ACTTCCAAAG CTGGAAATCC TGTTTTTTAT TATGTCACAC GAAGGTTCAA GACTGGCCAG 
ATCAACGGGG ATTTGCTTAT ATACCACGTC TTACTAACTT TAAAGCCATA TTATGCAAAG 
CCATATGAGA TTGTAATGGA CCTCACTCAT GCCGGGCCTA GCAATCGCTT TAAGACAGAC 
TTCCTCTCCA AGTGGTTTGT TGTTTTCCCG GGCTTTGCTT ACGAGAACGT CTCTTCGGTC 
TACATCTACA ACTGTAATTC TTGGGTTCGG GAGTACACCA AATACCATGA GAGACTGCTC 
ACGGGACTCA AAGGCAGCAA AAGACTCTTT TTCATAGACT CTCCCTCAAA ACTGGCTGAG 
CATATCGATT ATGATCAGCA AAAACTGCCA GCCGCGACCT TGGCTCTGGA TGAAGACCTC 
AAGGTGTACA ACAGTGCTCT CAAGCTGGCT CACAAGGACA CCAAGGTGAC CCTCAAGGTT 
GGCTCTACTG CGATCCAAGT GACGTCATCT GAGCGAACTA AAGTCCTGGG ACAGACGGTT 
TTTCTGAATG ATATTTACTA TGCCTCTGAG ATCGAAGAAA TCTGTCTTGT GGATGAAAAC 
CAGTTCACTC TGACCATTTC CAATCAGGGC ACTCCCCTCA CCTTCATGCA TCAAGAGTGT 
GAAGGCATTG TGGCGTCCAT CATTCACATC CGAACTCGAT GGGAGCTGTC CCAGCCAGAT 
TCTATCCCCC AGCACACTAA GATCCGGCCA AAGGACGTTC CCGGAACACT TTTGAATATC 
GCATTACTTA ACTTGGGTAG TTCGGACCCA AGTTTACGGT CAGCCGCCTA TAATCTTCTC 
TGTGCCTTAA CCTGTACCTT TAATTTAAAA ATTGAGGGCC AATTACTAGA AACATCAGGT 
CTGTGTATAC CTGCCAACAA TACCCTTTTT ATTGTCTCTA TTAGTAAAAC CCTGGCAGCT 
AATGAGCCAC ACCTCACCTT AGAGTTTTTG GAAGAGTGCA TTTCCGGATT TAGCAAATCC 
AGTATTGAAC TGAAGCACCT GTGTCTAGAA TACATGACTC CCTGGCTATC AAATCTAGTC 
CGTTTCTGTA AACATAATGA TGATGCCAAA CGACAGAGAG TCACTGCTAT TCTTGATAAA 
CTTATAACAA TGACCATAAA TGAAAAGCAG ATGTACCCAT CGATCCAAGC CAAGATATGG 
GGAAGCCTTG GGCAGATTAC AGATCTGCTC GATGTAGTGC TGGACAGCTT TATCAAAACC 
AGTGCCACAG GTGGTTTAGG ATCCATCAAG GCTGAAGTGA TGGCTGACAC GGCCGTGGCT 
CTGGCTTCTG GGAACGTCAA ACTAGTTTCG AGCAAGGTGA TTGGAAGAAT GTGCAAAATC 
ATTGACAAGA CCTGCCTCTC TCCAACTCCT ACCTTAGAGC AGCACCTCAT GTGGGATGAT 
ATCGCCATTT TAGCACGCTA CATGCTAATG CTGTCCTTCA ATAATTCTCT GGATGTGGCG 
GCTCATCTTC CCTACCTCTT CCATGTGGTG ACTCTGTTGG TGGCCACAGG TCCCTTGTCC 
CTTCGTGCTT CCACACACGG ACTGGTCATC AATATCATCC ATTCTCTGTG CACGTGCTCA 
CAGCTTCACT TTAGTGAAGA GACCAAGCAA GTTTTACGAC TGAGTCTGAC TGAGTTCTCA 
CTACCTAAGT TTTATTTGCT GTTCGGCATC AGCAAAGTCA AATCAGCTGC CGTGATAGCC 
TTCCGGTCCA GTTACCGGGA CAGGTCTTTC TCCCCTGGCT CCTATGAGAG GGAGACCTTT 
GCCCTGGCCT CCTTGGAAAC AGTCACAGAA GCTCTCTTGG AGATCATGGA GGCTTGTATG 
AGAGATATTC CTGCATGCAA GTGGCTGGAC CAGTGGACAG AGCTAGCCCA GAGGTTTGCG 
TTCCAGTACA ATCCCTCCCT GCAGCCACGA GCTCTGGTTG TGTTTGGTTG TATCAGCAAA 
AGAGTGTCCC ATGGGCAGAT CAAGCAGATT ATCCGCATTC TTAGCAAGGG ACTTGAGAGC 
TGCCTAAAAG GACCTGATAA TTATAACAGT CAAGTTCTGA TTGAAGCTAC CGTGATAGCT 
CTAACCAAAT TACAACCACT TCTTAATAAG CAGGAGTCCC CTCTGCACCG AGCTCTCTTC 
TGGGTGGCCG TTGCCGTGCT GCAGCTGGAT GAAGTGGACC TGTACTCGGC GGGCACTGCC 
CTCCTCGAAC AGAACCTGCA CACCTTAGAC AGCCTCCTGC ACATCTTCAA CGACCAGAGC 
CCAGAAGAAG TGTTCATGGA GATCCGGCAG CCTCTGGAGT GGCACTGCAA GCAGATGGAC 
CACTTTGTGG GGCTCAACTT TAACTCCAGC TTTAACTTTG CCCTGGTCGG GCACCTGCTG 
AAGGGATTTC GGCATCCTTC ACCTGTCACT GTTGCAAGAA CTGTGAGGAT TTTGCACACC 
CTACTAGCAT TGGTTAACAA GCATAGGAAT TGTGACAAGT TTGAGGTGAA CACCCAGAGT 
GTGGCTTACT TAGCAGCTTT ACTTACTGTG TCTGAGGAGG TTCGAAGTCG CTGCAGCTTA 
AAGCATAGGA AATCACTCCT ACTAACTGAT ATCACAATGG AAAATGTGCC TATGGATATA 
TATCCCATCT ATCACAGTGA CCCCAGCCTT AGGACACTAA AAGAAACACA ACCGTGGTCC 
TCTCCAAAGG GATCTGAGAG ACATCTTGCA GCCAGCTACC CTCCTGTGGG CCAAACTAGC 
CCCCGAACCA GAAAATCCAT GAGCCTGGAT ATGGGGCAGC CTTCCCAGGC CAACACAAAG 
AAGTTGCTTG GGACAAGGAA AAGCTTCGAT CATTTGATCT CTGATGCCAA AGCTCCCAAA 
AGGCAAGAAC AGGAGTCTGG AGTCACCACG CCCCCCAAGA TGCGGAGAGT GGCCGAGAAC 
GACTACGAGA TGGAAACCCA GAGAATCTCC CCTCAGCCAC ACCCGCCTCT GCGGAAAGTG 
TCCGTGTCCG AATCCAATGT GCTGCTGGAC GAGGAAGTGC TCACAGACCC CAAAACCCAG 
GCTCTTCTTC TCACTGTCCT GGCTACTCTG GTGAAGTACA CTGCGGACGA GTTTGACCAG 
CGCATTCTCT ATGAGTACTT AGCGGAGGCC AGTGTTGTCT TCCCAAAGGT TTTTCCAGTG 
GTACATAATT TGTTGGACTC AAAGATAAAT ACTCTGCTCT CATTATGCCA AGACCCAAAT 
TTGCTAAACC CGGTTCATGG AATTGTGCAG AGTGTGGTCT ACCACGAAGA GGCCCCGCCG 
CAGTACCAGA CGGCGTACCT CCAGAGTTTC GGTTTTAATG GTCTGTGGAG GTTTGCAGGA 
CCATTTTCTA AGCAGACGCA GATCCCAGAC TATGCCGAGC TCATCGTGAA GTTTCTCGAT 
GCCTTGATTG ACACGTATCT GCCTGGAATT GATGAGGAAG CCAGTGAGGA GTCCCTCCTG 
ACACCCACCT CTCCCTACCC GCCCGCGGTG CAGAGCCAGC TTAGCATCAC TGCGAACCTC 
AACCTCTCTA ACTCCATGAC CTCACTGGCA ACTTCCCAGC ATTCCCCAGG CATCGACAAG 
GAGAATGTGG AGCTCTCGCC AACCACCGGG CACTGCAGCG GCGGGAGGAC GCGCCACGGC 
TCCGCCAGCC AGGTCCAGAA GCAAAGGAGT GCCGGCAGCT TCAAGAGGCA CAGCATCAAG 
AAGATCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967651.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823511.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X12, mRNA.

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CloneID	OSa234699
Clone ID Related Accession (Same CDS sequence)	XM_031967651.1
Accession Version	XM_031967651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGAAAA TGTTTCTCGA CAATCTCAGA 
AGAGCGCTGG CCGGCCACAG TGGGAGCAGG CAGCTGACAG AGAGTGCCGC GATCGCTTGT 
GTTAAACTGT GCAAAGCCAG CACCTACATC AACTGGGAGG ATAACTCGGT CATTTTCCTG 
CTCGTCCAAT CCATGGTGGT GGATCTGAAG AACTTGCTCT TTAATCCCAG TAAGCCCTTC 
TCCAGAGGCA GCCAGAATGC TGATGTGGAC CTCATGATAG ACTGCCTGGT TTCTTGCTTT 
CGCATCAGCC CTCACAACAA CCAACACTTT AAGATCTGTT TGGCCCAGAA CTCCCCATCT 
ACATTTCATT ATGTGCTGGT AAACTCCCTC CATCGAATTA TCACCAATAG CACTTTCAAG 
CATGGACTTG GCACTGCTTC CGCCCTGGAT TGGTGGCCCA AGATTGATGC TGTGTATTGC 
CATTCTGTAG AACTTCGAAA CATGTTTGGT GAAACGCTTC ACAAGGCCGT GCAGGGCTGT 
GGGCCTCACC CCGCGATACG AATGGCGCCG AGTCTTACAT TTAAAGAAAA AATGACAAGC 
CTTAAATTTA AAGAAAAACC TACGGACTTG GAAACAAGAA GTTATAAATA CCTCTTATTA 
TCCATGGTGA AACTAATCCA TGCAGATCCA AAGCTGTTGC TATGTAATCC AAGAAAACAA 
GGGCCTGAAA CTCAGGGGAG TACAGCAGAG TTAATCACAG GCCTTGTGCA GCTCGTCCCA 
CAGTCCAGCA TGCCAGAAAT AGCTCAAGAA GCAATGGAGG CTTTGCTGGT TCTTCATCAG 
TTGGATAGTA TTGACTTATG GAATCCAGAT TCACCTATAG AAACATTCTG GGAGATTAGC 
TCACAAATGC TTTTTTATAT CTGTAAAAAG CTAACTACCC ATCAGATGCT TAGCAGTACA 
GAAATTCTCA AGTGGTTGCG AGAAATTTTG ATCTGTAGGA ACAAATATCT TCTTAAAAAT 
AAGCAGTCAG ATAGGAGTTC TTCCCATTTC CTCTACCTTT ATGGGGCAGG CTATGATGTC 
TCTTCTGGTG GAGGTACCAG TCATATCTCT CTAGACCACG AGGAGTTTAT GCGTACCTGT 
GCTCATGGAC CATCTCTCAG GAAAGTGAAA GGAAGCTCCT CAATGGACGG TGCAATCGGG 
TCCAGCGGGA CGCCCATCTG CCGCCAGGCC CAGACTAAGC TGGAGGTTGC TCTGTACATG 
TTTCTGTGGA GTCCCGACAT TGAAGCCGTG CTCGTGGCCA TGTCCTGCTT TCGACACCTG 
TGTGAGGAAG CCGACATCAG GTGTGGCATA GATGAGATCT CGGTGCACCA TTTCTTGCCA 
AACTATAACA CCTTCATGGA GTTCGCATCT GTCAGCAACA TGATGTCGAC AGGAAGAGCA 
GCTCTTCAGA AAAGAGTGAT GGCCCTGCTC AGGCGCATCG AACACCCCAC CGCTGGAAAC 
ACCGAGGCTT GGGAAGATAC TTACGCCAAA TGGGAGCAAG CTACAAAATC AATCCTCAAT 
TATCCGAAAG CCAAAATTGA AGATGGCCAG GTTGCTGAGA GTCTTCACAA GACCATTGTG 
AAGAGAAGAA TGTCTCACGT GAGTGGAGGG GGCTCCATAG ACTTGTGCGA CACTGACTCG 
CTACAGGAGT GGATCAACAT GACTGGGTTT CTCTGTGCCC TTGGTGGCGT GTGCCTACAG 
CAGCGGAGCA ATGCCGGCTT TGCCACCTAC AGCCCTCCCA TGGGCCCTAT CAGTGAACGC 
AAGGGCTCTC TCATTTCCAT GGTGTCCAGC GATGGAAACG CAGAGACGCC TGTCAGCAAG 
TTCATGGATC GGCTGCTCTC CTTGATGGTG TGTAACCACG AAAAAGTGGG GCTGCAGATA 
CGAAGCAATG TCAAGGACTT GTTGGGTCTT GAATTGAGTC CTGCCCTTTA TCCAATGCTG 
TTTAACAAAT TAAAAAATAC CATCAGCAAG TTTTTTGATC CTCAAGGACA GGTTTTATTG 
ACTGACACCA ACACCCAGTT TGTGGAGCAG ACCATAGCCA TAATGAAGAA CTTGCTTGAT 
AATCATGCTG AGGGTAGTTC TGAACACATG GGACAAGCTA GCATCGAAAC TATGATGTTA 
AATCTGGTCA GATATGTTAG AGTGTTTGGG AATTTGGTGC AGGCAATTCA GATCAAAACT 
AAGCTGTGCC AGCTGGTTCA AGTAATGATG GAGAGGAGAG ATGACCTTTC ATTTTGCCAA 
GAGATGAAAT TTAGGAATAA AATGGTAGAA TATCTGACAG ATTGGGTCAT GGGAACATCT 
AACCAAGCTG CAGATGAAGA TGTGAAATGT CTAACTAGAG ATTTGGACCA GGCAAGCATG 
GAAGCAGTGG TGTCATTGCT CGCTGGGCTC CCTCTGCAGC CTGAGGAAGG CGATGGGGTG 
GAATTGATGG AAGCCAAATC CCAGCTGTTC CTTAAGTATT TCACCCTCTT TATGAACCTT 
CTTAATGACT GCAGTGAGGT CGAAGAAGAA AATGTCCAGA TTGGGGGCAG GAAGCGCGGC 
ATGTCGCGAA GGCTGGCGTC CCTGCGCCAC TGCACCGTGC TGGCAATGTC CAACCTGCTC 
AATGCGAATG TAGACAGCGG CCTGATGCAC TCTATAGTGC TAGGATTGGG TTACCACAAA 
GATCTTCAGA CAAGAGCCAC CTTCATGGAA GTCCTCACAA AAATCCTTCA GCAAGGCACA 
GAGTTTGATA CGCTCGCAGA AACCGTGTTA GCTGATCGAT TTGAGAGACT TGTTGAGTTG 
GTCACGATGA TGGGTGATCA AGGAGAGCTC CCCATAGCCA TGGCTCTGGC CAACGTGGTG 
CCTTGTTCTC AGTGGGATGA ACTTGCTCGA GTCCTTGTTA CTCTCTTTGA TTCCCGACAC 
TTACTGTATC AGCTCCTTTG GAACATGTTT TCTAAGGAAG TTGAGCTGGC AGACTCCATG 
CAGACACTTT TTAGAGGAAA CAGCTTGGCC AGTAAAATCA TGACCTTCTG CTTTAAGGTC 
TACGGTGCTA CATACCTGCA GAAGCTTCTG GAGCCTTTAC TCCGCTTTGT GATCACATCC 
TCCGAATGGC AGCACGTCAG CTTCGAGGTC GATCCCACCA GGTTAGAGCA TACTGAGAGC 
CTCGAGGACA ACCAGCGGAA CCTTTTGCAA ATGACTGAGA AGTTCTTCCA CGCCATCATC 
AGTTCTTCCT CCGAGTTCCC CCCTCAGCTA CGCAGTGTGT GCCACTGCCT GTACCAGGCA 
ACTTGCCACT CTCTACTGAA TAAAGCTACC GTAAAAGAAA AAAAGGAAAA CAAAAAATCA 
GTTGTTAGCC AGAGATTCCC TCAGAACAGC ATTGGCGCCG TGGGGAGTGC CATGTTCCTG 
AGATTCATCA ACCCTGCCGT TGTCTCTCCC TACGAGGCAG GGATTTTAGA GAGAAAACCG 
CTCCCTAGAA TTGAAAGGGG GTTGAAGCTG ATGTCAAAGA TTCTTCAGAG CATTGCCAAT 
CATGTCCTTT TCACAAAAGA AGAGTATATG AGGCCTTTTA ACGATTTTGT GAAAAGCAAC 
TTTGATGTAG CACGGAGATT TTTCCTTGAT ATCGCATCTG ATTGTCCTGT AAGTGATGCC 
ATAAACCACA GCCTTTCCTT CATCAGCGAT GGCAATGTCT TGGCTTTGCA CCGTCTGCTC 
TGGAACAACC AAGAGAAAAT CGGCCAATAT CTTTCAAGCA ACAGAGATCA CAAAGCTGTT 
GGAAGGCGAC CTTTCGATAA GATGGCAACA CTGCTTGCCT ACCTGGGACC TCCAGAGCAC 
AAGCCTGTGG CTGACACACA CTGGTCCAGC CTAAATCTTA CCAGCTCCAA GTTTGAGGAA 
TTCATGACAA GGCACCAGGT ACATGAAAAA GAGGAATTCA AGGCTCTGAA AACATTAAAT 
ATCTTCTATC AAGCAGGCAC TTCCAAAGCT GGAAATCCTG TTTTTTATTA TGTCACACGA 
AGGTTCAAGA CTGGCCAGAT CAACGGGGAT TTGCTTATAT ACCACGTCTT ACTAACTTTA 
AAGCCATATT ATGCAAAGCC ATATGAGATT GTAATGGACC TCACTCATGC CGGGCCTAGC 
AATCGCTTTA AGACAGACTT CCTCTCCAAG TGGTTTGTTG TTTTCCCGGG CTTTGCTTAC 
GAGAACGTCT CTTCGGTCTA CATCTACAAC TGTAATTCTT GGGTTCGGGA GTACACCAAA 
TACCATGAGA GACTGCTCAC GGGACTCAAA GGCAGCAAAA GACTCTTTTT CATAGACTCT 
CCCTCAAAAC TGGCTGAGCA TATCGATTAT GATCAGCAAA AACTGCCAGC CGCGACCTTG 
GCTCTGGATG AAGACCTCAA GGTGTACAAC AGTGCTCTCA AGCTGGCTCA CAAGGACACC 
AAGGTGACCC TCAAGGTTGG CTCTACTGCG ATCCAAGTGA CGTCATCTGA GCGAACTAAA 
GTCCTGGGAC AGACGGTTTT TCTGAATGAT ATTTACTATG CCTCTGAGAT CGAAGAAATC 
TGTCTTGTGG ATGAAAACCA GTTCACTCTG ACCATTTCCA ATCAGGGCAC TCCCCTCACC 
TTCATGCATC AAGAGTGTGA AGGCATTGTG GCGTCCATCA TTCACATCCG AACTCGATGG 
GAGCTGTCCC AGCCAGATTC TATCCCCCAG CACACTAAGA TCCGGCCAAA GGACGTTCCC 
GGAACACTTT TGAATATCGC ATTACTTAAC TTGGGTAGTT CGGACCCAAG TTTACGGTCA 
GCCGCCTATA ATCTTCTCTG TGCCTTAACC TGTACCTTTA ATTTAAAAAT TGAGGGCCAA 
TTACTAGAAA CATCAGGTCT GTGTATACCT GCCAACAATA CCCTTTTTAT TGTCTCTATT 
AGTAAAACCC TGGCAGCTAA TGAGCCACAC CTCACCTTAG AGTTTTTGGA AGAGTGCATT 
TCCGGATTTA GCAAATCCAG TATTGAACTG AAGCACCTGT GTCTAGAATA CATGACTCCC 
TGGCTATCAA ATCTAGTCCG TTTCTGTAAA CATAATGATG ATGCCAAACG ACAGAGAGTC 
ACTGCTATTC TTGATAAACT TATAACAATG ACCATAAATG AAAAGCAGAT GTACCCATCG 
ATCCAAGCCA AGATATGGGG AAGCCTTGGG CAGATTACAG ATCTGCTCGA TGTAGTGCTG 
GACAGCTTTA TCAAAACCAG TGCCACAGGT GGTTTAGGAT CCATCAAGGC TGAAGTGATG 
GCTGACACGG CCGTGGCTCT GGCTTCTGGG AACGTCAAAC TAGTTTCGAG CAAGGTGATT 
GGAAGAATGT GCAAAATCAT TGACAAGACC TGCCTCTCTC CAACTCCTAC CTTAGAGCAG 
CACCTCATGT GGGATGATAT CGCCATTTTA GCACGCTACA TGCTAATGCT GTCCTTCAAT 
AATTCTCTGG ATGTGGCGGC TCATCTTCCC TACCTCTTCC ATGTGGTGAC TCTGTTGGTG 
GCCACAGGTC CCTTGTCCCT TCGTGCTTCC ACACACGGAC TGGTCATCAA TATCATCCAT 
TCTCTGTGCA CGTGCTCACA GCTTCACTTT AGTGAAGAGA CCAAGCAAGT TTTACGACTG 
AGTCTGACTG AGTTCTCACT ACCTAAGTTT TATTTGCTGT TCGGCATCAG CAAAGTCAAA 
TCAGCTGCCG TGATAGCCTT CCGGTCCAGT TACCGGGACA GGTCTTTCTC CCCTGGCTCC 
TATGAGAGGG AGACCTTTGC CCTGGCCTCC TTGGAAACAG TCACAGAAGC TCTCTTGGAG 
ATCATGGAGG CTTGTATGAG AGATATTCCT GCATGCAAGT GGCTGGACCA GTGGACAGAG 
CTAGCCCAGA GGTTTGCGTT CCAGTACAAT CCCTCCCTGC AGCCACGAGC TCTGGTTGTG 
TTTGGTTGTA TCAGCAAAAG AGTGTCCCAT GGGCAGATCA AGCAGATTAT CCGCATTCTT 
AGCAAGGGAC TTGAGAGCTG CCTAAAAGGA CCTGATAATT ATAACAGTCA AGTTCTGATT 
GAAGCTACCG TGATAGCTCT AACCAAATTA CAACCACTTC TTAATAAGCA GGAGTCCCCT 
CTGCACCGAG CTCTCTTCTG GGTGGCCGTT GCCGTGCTGC AGCTGGATGA AGTGGACCTG 
TACTCGGCGG GCACTGCCCT CCTCGAACAG AACCTGCACA CCTTAGACAG CCTCCTGCAC 
ATCTTCAACG ACCAGAGCCC AGAAGAAGTG TTCATGGAGA TCCGGCAGCC TCTGGAGTGG 
CACTGCAAGC AGATGGACCA CTTTGTGGGG CTCAACTTTA ACTCCAGCTT TAACTTTGCC 
CTGGTCGGGC ACCTGCTGAA GGGATTTCGG CATCCTTCAC CTGTCACTGT TGCAAGAACT 
GTGAGGATTT TGCACACCCT ACTAGCATTG GTTAACAAGC ATAGGAATTG TGACAAGTTT 
GAGGTGAACA CCCAGAGTGT GGCTTACTTA GCAGCTTTAC TTACTGTGTC TGAGGAGGTT 
CGAAGTCGCT GCAGCTTAAA GCATAGGAAA TCACTCCTAC TAACTGATAT CACAATGGAA 
AATGTGCCTA TGGATATATA TCCCATCTAT CACAGTGACC CCAGCCTTAG GACACTAAAA 
GAAACACAAC CGTGGTCCTC TCCAAAGGGA TCTGAGAGAC ATCTTGCAGC CAGCTACCCT 
CCTGTGGGCC AAACTAGCCC CCGAACCAGA AAATCCATGA GCCTGGATAT GGGGCAGCCT 
TCCCAGGCCA ACACAAAGAA GTTGCTTGGG ACAAGGAAAA GCTTCGATCA TTTGATCTCT 
GATGCCAAAG CTCCCAAAAG GCAAGAACAG GAGTCTGGAG TCACCACGCC CCCCAAGATG 
CGGAGAGTGG CCGAGAACGA CTACGAGATG GAAACCCAGA GAATCTCCCC TCAGCCACAC 
CCGCCTCTGC GGAAAGTGTC CGTGTCCGAA TCCAATGTGC TGCTGGACGA GGAAGTGCTC 
ACAGACCCCA AAACCCAGGC TCTTCTTCTC ACTGTCCTGG CTACTCTGGT GAAGTACACT 
GCGGACGAGT TTGACCAGCG CATTCTCTAT GAGTACTTAG CGGAGGCCAG TGTTGTCTTC 
CCAAAGGTTT TTCCAGTGGT ACATAATTTG TTGGACTCAA AGATAAATAC TCTGCTCTCA 
TTATGCCAAG ACCCAAATTT GCTAAACCCG GTTCATGGAA TTGTGCAGAG TGTGGTCTAC 
CACGAAGAGG CCCCGCCGCA GTACCAGACG GCGTACCTCC AGAGTTTCGG TTTTAATGGT 
CTGTGGAGGT TTGCAGGACC ATTTTCTAAG CAGACGCAGA TCCCAGACTA TGCCGAGCTC 
ATCGTGAAGT TTCTCGATGC CTTGATTGAC ACGTATCTGC CTGGAATTGA TGAGGAAGCC 
AGTGAGGAGT CCCTCCTGAC ACCCACCTCT CCCTACCCGC CCGCGGTGCA GAGCCAGCTT 
AGCATCACTG CGAACCTCAA CCTCTCTAAC TCCATGACCT CACTGGCAAC TTCCCAGCAT 
TCCCCAGGCA TCGACAAGGA GAATGTGGAG CTCTCGCCAA CCACCGGGCA CTGCAGCGGC 
GGGAGGACGC GCCACGGCTC CGCCAGCCAG GTCCAGAAGC AAAGGAGTGC CGGCAGCTTC 
AAGAGGCACA GCATCAAGAA GATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823511.1
CDS	9..8558
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGVSDLQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIVLGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKQESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967651.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967651.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGAAAA TGTTTCTCGA CAATCTCAGA 
AGAGCGCTGG CCGGCCACAG TGGGAGCAGG CAGCTGACAG AGAGTGCCGC GATCGCTTGT 
GTTAAACTGT GCAAAGCCAG CACCTACATC AACTGGGAGG ATAACTCGGT CATTTTCCTG 
CTCGTCCAAT CCATGGTGGT GGATCTGAAG AACTTGCTCT TTAATCCCAG TAAGCCCTTC 
TCCAGAGGCA GCCAGAATGC TGATGTGGAC CTCATGATAG ACTGCCTGGT TTCTTGCTTT 
CGCATCAGCC CTCACAACAA CCAACACTTT AAGATCTGTT TGGCCCAGAA CTCCCCATCT 
ACATTTCATT ATGTGCTGGT AAACTCCCTC CATCGAATTA TCACCAATAG CACTTTCAAG 
CATGGACTTG GCACTGCTTC CGCCCTGGAT TGGTGGCCCA AGATTGATGC TGTGTATTGC 
CATTCTGTAG AACTTCGAAA CATGTTTGGT GAAACGCTTC ACAAGGCCGT GCAGGGCTGT 
GGGCCTCACC CCGCGATACG AATGGCGCCG AGTCTTACAT TTAAAGAAAA AATGACAAGC 
CTTAAATTTA AAGAAAAACC TACGGACTTG GAAACAAGAA GTTATAAATA CCTCTTATTA 
TCCATGGTGA AACTAATCCA TGCAGATCCA AAGCTGTTGC TATGTAATCC AAGAAAACAA 
GGGCCTGAAA CTCAGGGGAG TACAGCAGAG TTAATCACAG GCCTTGTGCA GCTCGTCCCA 
CAGTCCAGCA TGCCAGAAAT AGCTCAAGAA GCAATGGAGG CTTTGCTGGT TCTTCATCAG 
TTGGATAGTA TTGACTTATG GAATCCAGAT TCACCTATAG AAACATTCTG GGAGATTAGC 
TCACAAATGC TTTTTTATAT CTGTAAAAAG CTAACTACCC ATCAGATGCT TAGCAGTACA 
GAAATTCTCA AGTGGTTGCG AGAAATTTTG ATCTGTAGGA ACAAATATCT TCTTAAAAAT 
AAGCAGTCAG ATAGGAGTTC TTCCCATTTC CTCTACCTTT ATGGGGCAGG CTATGATGTC 
TCTTCTGGTG GAGGTACCAG TCATATCTCT CTAGACCACG AGGAGTTTAT GCGTACCTGT 
GCTCATGGAC CATCTCTCAG GAAAGTGAAA GGAAGCTCCT CAATGGACGG TGCAATCGGG 
TCCAGCGGGA CGCCCATCTG CCGCCAGGCC CAGACTAAGC TGGAGGTTGC TCTGTACATG 
TTTCTGTGGA GTCCCGACAT TGAAGCCGTG CTCGTGGCCA TGTCCTGCTT TCGACACCTG 
TGTGAGGAAG CCGACATCAG GTGTGGCATA GATGAGATCT CGGTGCACCA TTTCTTGCCA 
AACTATAACA CCTTCATGGA GTTCGCATCT GTCAGCAACA TGATGTCGAC AGGAAGAGCA 
GCTCTTCAGA AAAGAGTGAT GGCCCTGCTC AGGCGCATCG AACACCCCAC CGCTGGAAAC 
ACCGAGGCTT GGGAAGATAC TTACGCCAAA TGGGAGCAAG CTACAAAATC AATCCTCAAT 
TATCCGAAAG CCAAAATTGA AGATGGCCAG GTTGCTGAGA GTCTTCACAA GACCATTGTG 
AAGAGAAGAA TGTCTCACGT GAGTGGAGGG GGCTCCATAG ACTTGTGCGA CACTGACTCG 
CTACAGGAGT GGATCAACAT GACTGGGTTT CTCTGTGCCC TTGGTGGCGT GTGCCTACAG 
CAGCGGAGCA ATGCCGGCTT TGCCACCTAC AGCCCTCCCA TGGGCCCTAT CAGTGAACGC 
AAGGGCTCTC TCATTTCCAT GGTGTCCAGC GATGGAAACG CAGAGACGCC TGTCAGCAAG 
TTCATGGATC GGCTGCTCTC CTTGATGGTG TGTAACCACG AAAAAGTGGG GCTGCAGATA 
CGAAGCAATG TCAAGGACTT GTTGGGTCTT GAATTGAGTC CTGCCCTTTA TCCAATGCTG 
TTTAACAAAT TAAAAAATAC CATCAGCAAG TTTTTTGATC CTCAAGGACA GGTTTTATTG 
ACTGACACCA ACACCCAGTT TGTGGAGCAG ACCATAGCCA TAATGAAGAA CTTGCTTGAT 
AATCATGCTG AGGGTAGTTC TGAACACATG GGACAAGCTA GCATCGAAAC TATGATGTTA 
AATCTGGTCA GATATGTTAG AGTGTTTGGG AATTTGGTGC AGGCAATTCA GATCAAAACT 
AAGCTGTGCC AGCTGGTTCA AGTAATGATG GAGAGGAGAG ATGACCTTTC ATTTTGCCAA 
GAGATGAAAT TTAGGAATAA AATGGTAGAA TATCTGACAG ATTGGGTCAT GGGAACATCT 
AACCAAGCTG CAGATGAAGA TGTGAAATGT CTAACTAGAG ATTTGGACCA GGCAAGCATG 
GAAGCAGTGG TGTCATTGCT CGCTGGGCTC CCTCTGCAGC CTGAGGAAGG CGATGGGGTG 
GAATTGATGG AAGCCAAATC CCAGCTGTTC CTTAAGTATT TCACCCTCTT TATGAACCTT 
CTTAATGACT GCAGTGAGGT CGAAGAAGAA AATGTCCAGA TTGGGGGCAG GAAGCGCGGC 
ATGTCGCGAA GGCTGGCGTC CCTGCGCCAC TGCACCGTGC TGGCAATGTC CAACCTGCTC 
AATGCGAATG TAGACAGCGG CCTGATGCAC TCTATAGTGC TAGGATTGGG TTACCACAAA 
GATCTTCAGA CAAGAGCCAC CTTCATGGAA GTCCTCACAA AAATCCTTCA GCAAGGCACA 
GAGTTTGATA CGCTCGCAGA AACCGTGTTA GCTGATCGAT TTGAGAGACT TGTTGAGTTG 
GTCACGATGA TGGGTGATCA AGGAGAGCTC CCCATAGCCA TGGCTCTGGC CAACGTGGTG 
CCTTGTTCTC AGTGGGATGA ACTTGCTCGA GTCCTTGTTA CTCTCTTTGA TTCCCGACAC 
TTACTGTATC AGCTCCTTTG GAACATGTTT TCTAAGGAAG TTGAGCTGGC AGACTCCATG 
CAGACACTTT TTAGAGGAAA CAGCTTGGCC AGTAAAATCA TGACCTTCTG CTTTAAGGTC 
TACGGTGCTA CATACCTGCA GAAGCTTCTG GAGCCTTTAC TCCGCTTTGT GATCACATCC 
TCCGAATGGC AGCACGTCAG CTTCGAGGTC GATCCCACCA GGTTAGAGCA TACTGAGAGC 
CTCGAGGACA ACCAGCGGAA CCTTTTGCAA ATGACTGAGA AGTTCTTCCA CGCCATCATC 
AGTTCTTCCT CCGAGTTCCC CCCTCAGCTA CGCAGTGTGT GCCACTGCCT GTACCAGGCA 
ACTTGCCACT CTCTACTGAA TAAAGCTACC GTAAAAGAAA AAAAGGAAAA CAAAAAATCA 
GTTGTTAGCC AGAGATTCCC TCAGAACAGC ATTGGCGCCG TGGGGAGTGC CATGTTCCTG 
AGATTCATCA ACCCTGCCGT TGTCTCTCCC TACGAGGCAG GGATTTTAGA GAGAAAACCG 
CTCCCTAGAA TTGAAAGGGG GTTGAAGCTG ATGTCAAAGA TTCTTCAGAG CATTGCCAAT 
CATGTCCTTT TCACAAAAGA AGAGTATATG AGGCCTTTTA ACGATTTTGT GAAAAGCAAC 
TTTGATGTAG CACGGAGATT TTTCCTTGAT ATCGCATCTG ATTGTCCTGT AAGTGATGCC 
ATAAACCACA GCCTTTCCTT CATCAGCGAT GGCAATGTCT TGGCTTTGCA CCGTCTGCTC 
TGGAACAACC AAGAGAAAAT CGGCCAATAT CTTTCAAGCA ACAGAGATCA CAAAGCTGTT 
GGAAGGCGAC CTTTCGATAA GATGGCAACA CTGCTTGCCT ACCTGGGACC TCCAGAGCAC 
AAGCCTGTGG CTGACACACA CTGGTCCAGC CTAAATCTTA CCAGCTCCAA GTTTGAGGAA 
TTCATGACAA GGCACCAGGT ACATGAAAAA GAGGAATTCA AGGCTCTGAA AACATTAAAT 
ATCTTCTATC AAGCAGGCAC TTCCAAAGCT GGAAATCCTG TTTTTTATTA TGTCACACGA 
AGGTTCAAGA CTGGCCAGAT CAACGGGGAT TTGCTTATAT ACCACGTCTT ACTAACTTTA 
AAGCCATATT ATGCAAAGCC ATATGAGATT GTAATGGACC TCACTCATGC CGGGCCTAGC 
AATCGCTTTA AGACAGACTT CCTCTCCAAG TGGTTTGTTG TTTTCCCGGG CTTTGCTTAC 
GAGAACGTCT CTTCGGTCTA CATCTACAAC TGTAATTCTT GGGTTCGGGA GTACACCAAA 
TACCATGAGA GACTGCTCAC GGGACTCAAA GGCAGCAAAA GACTCTTTTT CATAGACTCT 
CCCTCAAAAC TGGCTGAGCA TATCGATTAT GATCAGCAAA AACTGCCAGC CGCGACCTTG 
GCTCTGGATG AAGACCTCAA GGTGTACAAC AGTGCTCTCA AGCTGGCTCA CAAGGACACC 
AAGGTGACCC TCAAGGTTGG CTCTACTGCG ATCCAAGTGA CGTCATCTGA GCGAACTAAA 
GTCCTGGGAC AGACGGTTTT TCTGAATGAT ATTTACTATG CCTCTGAGAT CGAAGAAATC 
TGTCTTGTGG ATGAAAACCA GTTCACTCTG ACCATTTCCA ATCAGGGCAC TCCCCTCACC 
TTCATGCATC AAGAGTGTGA AGGCATTGTG GCGTCCATCA TTCACATCCG AACTCGATGG 
GAGCTGTCCC AGCCAGATTC TATCCCCCAG CACACTAAGA TCCGGCCAAA GGACGTTCCC 
GGAACACTTT TGAATATCGC ATTACTTAAC TTGGGTAGTT CGGACCCAAG TTTACGGTCA 
GCCGCCTATA ATCTTCTCTG TGCCTTAACC TGTACCTTTA ATTTAAAAAT TGAGGGCCAA 
TTACTAGAAA CATCAGGTCT GTGTATACCT GCCAACAATA CCCTTTTTAT TGTCTCTATT 
AGTAAAACCC TGGCAGCTAA TGAGCCACAC CTCACCTTAG AGTTTTTGGA AGAGTGCATT 
TCCGGATTTA GCAAATCCAG TATTGAACTG AAGCACCTGT GTCTAGAATA CATGACTCCC 
TGGCTATCAA ATCTAGTCCG TTTCTGTAAA CATAATGATG ATGCCAAACG ACAGAGAGTC 
ACTGCTATTC TTGATAAACT TATAACAATG ACCATAAATG AAAAGCAGAT GTACCCATCG 
ATCCAAGCCA AGATATGGGG AAGCCTTGGG CAGATTACAG ATCTGCTCGA TGTAGTGCTG 
GACAGCTTTA TCAAAACCAG TGCCACAGGT GGTTTAGGAT CCATCAAGGC TGAAGTGATG 
GCTGACACGG CCGTGGCTCT GGCTTCTGGG AACGTCAAAC TAGTTTCGAG CAAGGTGATT 
GGAAGAATGT GCAAAATCAT TGACAAGACC TGCCTCTCTC CAACTCCTAC CTTAGAGCAG 
CACCTCATGT GGGATGATAT CGCCATTTTA GCACGCTACA TGCTAATGCT GTCCTTCAAT 
AATTCTCTGG ATGTGGCGGC TCATCTTCCC TACCTCTTCC ATGTGGTGAC TCTGTTGGTG 
GCCACAGGTC CCTTGTCCCT TCGTGCTTCC ACACACGGAC TGGTCATCAA TATCATCCAT 
TCTCTGTGCA CGTGCTCACA GCTTCACTTT AGTGAAGAGA CCAAGCAAGT TTTACGACTG 
AGTCTGACTG AGTTCTCACT ACCTAAGTTT TATTTGCTGT TCGGCATCAG CAAAGTCAAA 
TCAGCTGCCG TGATAGCCTT CCGGTCCAGT TACCGGGACA GGTCTTTCTC CCCTGGCTCC 
TATGAGAGGG AGACCTTTGC CCTGGCCTCC TTGGAAACAG TCACAGAAGC TCTCTTGGAG 
ATCATGGAGG CTTGTATGAG AGATATTCCT GCATGCAAGT GGCTGGACCA GTGGACAGAG 
CTAGCCCAGA GGTTTGCGTT CCAGTACAAT CCCTCCCTGC AGCCACGAGC TCTGGTTGTG 
TTTGGTTGTA TCAGCAAAAG AGTGTCCCAT GGGCAGATCA AGCAGATTAT CCGCATTCTT 
AGCAAGGGAC TTGAGAGCTG CCTAAAAGGA CCTGATAATT ATAACAGTCA AGTTCTGATT 
GAAGCTACCG TGATAGCTCT AACCAAATTA CAACCACTTC TTAATAAGCA GGAGTCCCCT 
CTGCACCGAG CTCTCTTCTG GGTGGCCGTT GCCGTGCTGC AGCTGGATGA AGTGGACCTG 
TACTCGGCGG GCACTGCCCT CCTCGAACAG AACCTGCACA CCTTAGACAG CCTCCTGCAC 
ATCTTCAACG ACCAGAGCCC AGAAGAAGTG TTCATGGAGA TCCGGCAGCC TCTGGAGTGG 
CACTGCAAGC AGATGGACCA CTTTGTGGGG CTCAACTTTA ACTCCAGCTT TAACTTTGCC 
CTGGTCGGGC ACCTGCTGAA GGGATTTCGG CATCCTTCAC CTGTCACTGT TGCAAGAACT 
GTGAGGATTT TGCACACCCT ACTAGCATTG GTTAACAAGC ATAGGAATTG TGACAAGTTT 
GAGGTGAACA CCCAGAGTGT GGCTTACTTA GCAGCTTTAC TTACTGTGTC TGAGGAGGTT 
CGAAGTCGCT GCAGCTTAAA GCATAGGAAA TCACTCCTAC TAACTGATAT CACAATGGAA 
AATGTGCCTA TGGATATATA TCCCATCTAT CACAGTGACC CCAGCCTTAG GACACTAAAA 
GAAACACAAC CGTGGTCCTC TCCAAAGGGA TCTGAGAGAC ATCTTGCAGC CAGCTACCCT 
CCTGTGGGCC AAACTAGCCC CCGAACCAGA AAATCCATGA GCCTGGATAT GGGGCAGCCT 
TCCCAGGCCA ACACAAAGAA GTTGCTTGGG ACAAGGAAAA GCTTCGATCA TTTGATCTCT 
GATGCCAAAG CTCCCAAAAG GCAAGAACAG GAGTCTGGAG TCACCACGCC CCCCAAGATG 
CGGAGAGTGG CCGAGAACGA CTACGAGATG GAAACCCAGA GAATCTCCCC TCAGCCACAC 
CCGCCTCTGC GGAAAGTGTC CGTGTCCGAA TCCAATGTGC TGCTGGACGA GGAAGTGCTC 
ACAGACCCCA AAACCCAGGC TCTTCTTCTC ACTGTCCTGG CTACTCTGGT GAAGTACACT 
GCGGACGAGT TTGACCAGCG CATTCTCTAT GAGTACTTAG CGGAGGCCAG TGTTGTCTTC 
CCAAAGGTTT TTCCAGTGGT ACATAATTTG TTGGACTCAA AGATAAATAC TCTGCTCTCA 
TTATGCCAAG ACCCAAATTT GCTAAACCCG GTTCATGGAA TTGTGCAGAG TGTGGTCTAC 
CACGAAGAGG CCCCGCCGCA GTACCAGACG GCGTACCTCC AGAGTTTCGG TTTTAATGGT 
CTGTGGAGGT TTGCAGGACC ATTTTCTAAG CAGACGCAGA TCCCAGACTA TGCCGAGCTC 
ATCGTGAAGT TTCTCGATGC CTTGATTGAC ACGTATCTGC CTGGAATTGA TGAGGAAGCC 
AGTGAGGAGT CCCTCCTGAC ACCCACCTCT CCCTACCCGC CCGCGGTGCA GAGCCAGCTT 
AGCATCACTG CGAACCTCAA CCTCTCTAAC TCCATGACCT CACTGGCAAC TTCCCAGCAT 
TCCCCAGGCA TCGACAAGGA GAATGTGGAG CTCTCGCCAA CCACCGGGCA CTGCAGCGGC 
GGGAGGACGC GCCACGGCTC CGCCAGCCAG GTCCAGAAGC AAAGGAGTGC CGGCAGCTTC 
AAGAGGCACA GCATCAAGAA GATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967652.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823512.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X13, mRNA.

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CloneID	OSa234700
Clone ID Related Accession (Same CDS sequence)	XM_031967652.1
Accession Version	XM_031967652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGCAGTCA 
GATAGGAGTT CTTCCCATTT CCTCTACCTT TATGGGGCAG GCTATGATGT CTCTTCTGGT 
GGAGGTACCA GTCATATCTC TCTAGACCAC GAGGAGTTTA TGCGTACCTG TGCTCATGGA 
CCATCTCTCA GGAAAGTGAA AGGAAGCTCC TCAATGGACG GTGCAATCGG GTCCAGCGGG 
ACGCCCATCT GCCGCCAGGC CCAGACTAAG CTGGAGGTTG CTCTGTACAT GTTTCTGTGG 
AGTCCCGACA TTGAAGCCGT GCTCGTGGCC ATGTCCTGCT TTCGACACCT GTGTGAGGAA 
GCCGACATCA GGTGTGGCAT AGATGAGATC TCGGTGCACC ATTTCTTGCC AAACTATAAC 
ACCTTCATGG AGTTCGCATC TGTCAGCAAC ATGATGTCGA CAGGAAGAGC AGCTCTTCAG 
AAAAGAGTGA TGGCCCTGCT CAGGCGCATC GAACACCCCA CCGCTGGAAA CACCGAGGCT 
TGGGAAGATA CTTACGCCAA ATGGGAGCAA GCTACAAAAT CAATCCTCAA TTATCCGAAA 
GCCAAAATTG AAGATGGCCA GGTTGCTGAG AGTCTTCACA AGACCATTGT GAAGAGAAGA 
ATGTCTCACG TGAGTGGAGG GGGCTCCATA GACTTGTGCG ACACTGACTC GCTACAGGAG 
TGGATCAACA TGACTGGGTT TCTCTGTGCC CTTGGTGGCG TGTGCCTACA GCAGCGGAGC 
AATGCCGGCT TTGCCACCTA CAGCCCTCCC ATGGGCCCTA TCAGTGAACG CAAGGGCTCT 
CTCATTTCCA TGGTGTCCAG CGATGGAAAC GCAGAGACGC CTGTCAGCAA GTTCATGGAT 
CGGCTGCTCT CCTTGATGGT GTGTAACCAC GAAAAAGTGG GGCTGCAGAT ACGAAGCAAT 
GTCAAGGACT TGTTGGGTCT TGAATTGAGT CCTGCCCTTT ATCCAATGCT GTTTAACAAA 
TTAAAAAATA CCATCAGCAA GTTTTTTGAT CCTCAAGGAC AGGTTTTATT GACTGACACC 
AACACCCAGT TTGTGGAGCA GACCATAGCC ATAATGAAGA ACTTGCTTGA TAATCATGCT 
GAGGGTAGTT CTGAACACAT GGGACAAGCT AGCATCGAAA CTATGATGTT AAATCTGGTC 
AGATATGTTA GAGTGTTTGG GAATTTGGTG CAGGCAATTC AGATCAAAAC TAAGCTGTGC 
CAGCTGGTTC AAGTAATGAT GGAGAGGAGA GATGACCTTT CATTTTGCCA AGAGATGAAA 
TTTAGGAATA AAATGGTAGA ATATCTGACA GATTGGGTCA TGGGAACATC TAACCAAGCT 
GCAGATGAAG ATGTGAAATG TCTAACTAGA GATTTGGACC AGGCAAGCAT GGAAGCAGTG 
GTGTCATTGC TCGCTGGGCT CCCTCTGCAG CCTGAGGAAG GCGATGGGGT GGAATTGATG 
GAAGCCAAAT CCCAGCTGTT CCTTAAGTAT TTCACCCTCT TTATGAACCT TCTTAATGAC 
TGCAGTGAGG TCGAAGAAGA AAATGTCCAG ATTGGGGGCA GGAAGCGCGG CATGTCGCGA 
AGGCTGGCGT CCCTGCGCCA CTGCACCGTG CTGGCAATGT CCAACCTGCT CAATGCGAAT 
GTAGACAGCG GCCTGATGCA CTCTATAGTG CTAGGATTGG GTTACCACAA AGATCTTCAG 
ACAAGAGCCA CCTTCATGGA AGTCCTCACA AAAATCCTTC AGCAAGGCAC AGAGTTTGAT 
ACGCTCGCAG AAACCGTGTT AGCTGATCGA TTTGAGAGAC TTGTTGAGTT GGTCACGATG 
ATGGGTGATC AAGGAGAGCT CCCCATAGCC ATGGCTCTGG CCAACGTGGT GCCTTGTTCT 
CAGTGGGATG AACTTGCTCG AGTCCTTGTT ACTCTCTTTG ATTCCCGACA CTTACTGTAT 
CAGCTCCTTT GGAACATGTT TTCTAAGGAA GTTGAGCTGG CAGACTCCAT GCAGACACTT 
TTTAGAGGAA ACAGCTTGGC CAGTAAAATC ATGACCTTCT GCTTTAAGGT CTACGGTGCT 
ACATACCTGC AGAAGCTTCT GGAGCCTTTA CTCCGCTTTG TGATCACATC CTCCGAATGG 
CAGCACGTCA GCTTCGAGGT CGATCCCACC AGGTTAGAGC ATACTGAGAG CCTCGAGGAC 
AACCAGCGGA ACCTTTTGCA AATGACTGAG AAGTTCTTCC ACGCCATCAT CAGTTCTTCC 
TCCGAGTTCC CCCCTCAGCT ACGCAGTGTG TGCCACTGCC TGTACCAGGC AACTTGCCAC 
TCTCTACTGA ATAAAGCTAC CGTAAAAGAA AAAAAGGAAA ACAAAAAATC AGTTGTTAGC 
CAGAGATTCC CTCAGAACAG CATTGGCGCC GTGGGGAGTG CCATGTTCCT GAGATTCATC 
AACCCTGCCG TTGTCTCTCC CTACGAGGCA GGGATTTTAG AGAGAAAACC GCTCCCTAGA 
ATTGAAAGGG GGTTGAAGCT GATGTCAAAG ATTCTTCAGA GCATTGCCAA TCATGTCCTT 
TTCACAAAAG AAGAGTATAT GAGGCCTTTT AACGATTTTG TGAAAAGCAA CTTTGATGTA 
GCACGGAGAT TTTTCCTTGA TATCGCATCT GATTGTCCTG TAAGTGATGC CATAAACCAC 
AGCCTTTCCT TCATCAGCGA TGGCAATGTC TTGGCTTTGC ACCGTCTGCT CTGGAACAAC 
CAAGAGAAAA TCGGCCAATA TCTTTCAAGC AACAGAGATC ACAAAGCTGT TGGAAGGCGA 
CCTTTCGATA AGATGGCAAC ACTGCTTGCC TACCTGGGAC CTCCAGAGCA CAAGCCTGTG 
GCTGACACAC ACTGGTCCAG CCTAAATCTT ACCAGCTCCA AGTTTGAGGA ATTCATGACA 
AGGCACCAGG TACATGAAAA AGAGGAATTC AAGGCTCTGA AAACATTAAA TATCTTCTAT 
CAAGCAGGCA CTTCCAAAGC TGGAAATCCT GTTTTTTATT ATGTCACACG AAGGTTCAAG 
ACTGGCCAGA TCAACGGGGA TTTGCTTATA TACCACGTCT TACTAACTTT AAAGCCATAT 
TATGCAAAGC CATATGAGAT TGTAATGGAC CTCACTCATG CCGGGCCTAG CAATCGCTTT 
AAGACAGACT TCCTCTCCAA GTGGTTTGTT GTTTTCCCGG GCTTTGCTTA CGAGAACGTC 
TCTTCGGTCT ACATCTACAA CTGTAATTCT TGGGTTCGGG AGTACACCAA ATACCATGAG 
AGACTGCTCA CGGGACTCAA AGGCAGCAAA AGACTCTTTT TCATAGACTC TCCCTCAAAA 
CTGGCTGAGC ATATCGATTA TGATCAGCAA AAACTGCCAG CCGCGACCTT GGCTCTGGAT 
GAAGACCTCA AGGTGTACAA CAGTGCTCTC AAGCTGGCTC ACAAGGACAC CAAGGTGACC 
CTCAAGGTTG GCTCTACTGC GATCCAAGTG ACGTCATCTG AGCGAACTAA AGTCCTGGGA 
CAGACGGTTT TTCTGAATGA TATTTACTAT GCCTCTGAGA TCGAAGAAAT CTGTCTTGTG 
GATGAAAACC AGTTCACTCT GACCATTTCC AATCAGGGCA CTCCCCTCAC CTTCATGCAT 
CAAGAGTGTG AAGGCATTGT GGCGTCCATC ATTCACATCC GAACTCGATG GGAGCTGTCC 
CAGCCAGATT CTATCCCCCA GCACACTAAG ATCCGGCCAA AGGACGTTCC CGGAACACTT 
TTGAATATCG CATTACTTAA CTTGGGTAGT TCGGACCCAA GTTTACGTAA AACCCTGGCA 
GCTAATGAGC CACACCTCAC CTTAGAGTTT TTGGAAGAGT GCATTTCCGG ATTTAGCAAA 
TCCAGTATTG AACTGAAGCA CCTGTGTCTA GAATACATGA CTCCCTGGCT ATCAAATCTA 
GTCCGTTTCT GTAAACATAA TGATGATGCC AAACGACAGA GAGTCACTGC TATTCTTGAT 
AAACTTATAA CAATGACCAT AAATGAAAAG CAGATGTACC CATCGATCCA AGCCAAGATA 
TGGGGAAGCC TTGGGCAGAT TACAGATCTG CTCGATGTAG TGCTGGACAG CTTTATCAAA 
ACCAGTGCCA CAGGTGGTTT AGGATCCATC AAGGCTGAAG TGATGGCTGA CACGGCCGTG 
GCTCTGGCTT CTGGGAACGT CAAACTAGTT TCGAGCAAGG TGATTGGAAG AATGTGCAAA 
ATCATTGACA AGACCTGCCT CTCTCCAACT CCTACCTTAG AGCAGCACCT CATGTGGGAT 
GATATCGCCA TTTTAGCACG CTACATGCTA ATGCTGTCCT TCAATAATTC TCTGGATGTG 
GCGGCTCATC TTCCCTACCT CTTCCATGTG GTGACTCTGT TGGTGGCCAC AGGTCCCTTG 
TCCCTTCGTG CTTCCACACA CGGACTGGTC ATCAATATCA TCCATTCTCT GTGCACGTGC 
TCACAGCTTC ACTTTAGTGA AGAGACCAAG CAAGTTTTAC GACTGAGTCT GACTGAGTTC 
TCACTACCTA AGTTTTATTT GCTGTTCGGC ATCAGCAAAG TCAAATCAGC TGCCGTGATA 
GCCTTCCGGT CCAGTTACCG GGACAGGTCT TTCTCCCCTG GCTCCTATGA GAGGGAGACC 
TTTGCCCTGG CCTCCTTGGA AACAGTCACA GAAGCTCTCT TGGAGATCAT GGAGGCTTGT 
ATGAGAGATA TTCCTGCATG CAAGTGGCTG GACCAGTGGA CAGAGCTAGC CCAGAGGTTT 
GCGTTCCAGT ACAATCCCTC CCTGCAGCCA CGAGCTCTGG TTGTGTTTGG TTGTATCAGC 
AAAAGAGTGT CCCATGGGCA GATCAAGCAG ATTATCCGCA TTCTTAGCAA GGGACTTGAG 
AGCTGCCTAA AAGGACCTGA TAATTATAAC AGTCAAGTTC TGATTGAAGC TACCGTGATA 
GCTCTAACCA AATTACAACC ACTTCTTAAT AAGCAGGAGT CCCCTCTGCA CCGAGCTCTC 
TTCTGGGTGG CCGTTGCCGT GCTGCAGCTG GATGAAGTGG ACCTGTACTC GGCGGGCACT 
GCCCTCCTCG AACAGAACCT GCACACCTTA GACAGCCTCC TGCACATCTT CAACGACCAG 
AGCCCAGAAG AAGTGTTCAT GGAGATCCGG CAGCCTCTGG AGTGGCACTG CAAGCAGATG 
GACCACTTTG TGGGGCTCAA CTTTAACTCC AGCTTTAACT TTGCCCTGGT CGGGCACCTG 
CTGAAGGGAT TTCGGCATCC TTCACCTGTC ACTGTTGCAA GAACTGTGAG GATTTTGCAC 
ACCCTACTAG CATTGGTTAA CAAGCATAGG AATTGTGACA AGTTTGAGGT GAACACCCAG 
AGTGTGGCTT ACTTAGCAGC TTTACTTACT GTGTCTGAGG AGGTTCGAAG TCGCTGCAGC 
TTAAAGCATA GGAAATCACT CCTACTAACT GATATCACAA TGGAAAATGT GCCTATGGAT 
ATATATCCCA TCTATCACAG TGACCCCAGC CTTAGGACAC TAAAAGAAAC ACAACCGTGG 
TCCTCTCCAA AGGGATCTGA GAGACATCTT GCAGCCAGCT ACCCTCCTGT GGGCCAAACT 
AGCCCCCGAA CCAGAAAATC CATGAGCCTG GATATGGGGC AGCCTTCCCA GGCCAACACA 
AAGAAGTTGC TTGGGACAAG GAAAAGCTTC GATCATTTGA TCTCTGATGC CAAAGCTCCC 
AAAAGGCAAG AACAGGAGTC TGGAGTCACC ACGCCCCCCA AGATGCGGAG AGTGGCCGAG 
AACGACTACG AGATGGAAAC CCAGAGAATC TCCCCTCAGC CACACCCGCC TCTGCGGAAA 
GTGTCCGTGT CCGAATCCAA TGTGCTGCTG GACGAGGAAG TGCTCACAGA CCCCAAAACC 
CAGGCTCTTC TTCTCACTGT CCTGGCTACT CTGGTGAAGT ACACTGCGGA CGAGTTTGAC 
CAGCGCATTC TCTATGAGTA CTTAGCGGAG GCCAGTGTTG TCTTCCCAAA GGTTTTTCCA 
GTGGTACATA ATTTGTTGGA CTCAAAGATA AATACTCTGC TCTCATTATG CCAAGACCCA 
AATTTGCTAA ACCCGGTTCA TGGAATTGTG CAGAGTGTGG TCTACCACGA AGAGGCCCCG 
CCGCAGTACC AGACGGCGTA CCTCCAGAGT TTCGGTTTTA ATGGTCTGTG GAGGTTTGCA 
GGACCATTTT CTAAGCAGAC GCAGATCCCA GACTATGCCG AGCTCATCGT GAAGTTTCTC 
GATGCCTTGA TTGACACGTA TCTGCCTGGA ATTGATGAGG AAGCCAGTGA GGAGTCCCTC 
CTGACACCCA CCTCTCCCTA CCCGCCCGCG GTGCAGAGCC AGCTTAGCAT CACTGCGAAC 
CTCAACCTCT CTAACTCCAT GACCTCACTG GCAACTTCCC AGCATTCCCC AGGCATCGAC 
AAGGAGAATG TGGAGCTCTC GCCAACCACC GGGCACTGCA GCGGCGGGAG GACGCGCCAC 
GGCTCCGCCA GCCAGGTCCA GAAGCAAAGG AGTGCCGGCA GCTTCAAGAG GCACAGCATC 
AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823512.1
CDS	9..8543
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGVSDLQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKFSTLLTTITAWILSPWLCDTDVSIPLSCQITRPQSSFKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIVLGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKQESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967652.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967652.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGCAGTCA 
GATAGGAGTT CTTCCCATTT CCTCTACCTT TATGGGGCAG GCTATGATGT CTCTTCTGGT 
GGAGGTACCA GTCATATCTC TCTAGACCAC GAGGAGTTTA TGCGTACCTG TGCTCATGGA 
CCATCTCTCA GGAAAGTGAA AGGAAGCTCC TCAATGGACG GTGCAATCGG GTCCAGCGGG 
ACGCCCATCT GCCGCCAGGC CCAGACTAAG CTGGAGGTTG CTCTGTACAT GTTTCTGTGG 
AGTCCCGACA TTGAAGCCGT GCTCGTGGCC ATGTCCTGCT TTCGACACCT GTGTGAGGAA 
GCCGACATCA GGTGTGGCAT AGATGAGATC TCGGTGCACC ATTTCTTGCC AAACTATAAC 
ACCTTCATGG AGTTCGCATC TGTCAGCAAC ATGATGTCGA CAGGAAGAGC AGCTCTTCAG 
AAAAGAGTGA TGGCCCTGCT CAGGCGCATC GAACACCCCA CCGCTGGAAA CACCGAGGCT 
TGGGAAGATA CTTACGCCAA ATGGGAGCAA GCTACAAAAT CAATCCTCAA TTATCCGAAA 
GCCAAAATTG AAGATGGCCA GGTTGCTGAG AGTCTTCACA AGACCATTGT GAAGAGAAGA 
ATGTCTCACG TGAGTGGAGG GGGCTCCATA GACTTGTGCG ACACTGACTC GCTACAGGAG 
TGGATCAACA TGACTGGGTT TCTCTGTGCC CTTGGTGGCG TGTGCCTACA GCAGCGGAGC 
AATGCCGGCT TTGCCACCTA CAGCCCTCCC ATGGGCCCTA TCAGTGAACG CAAGGGCTCT 
CTCATTTCCA TGGTGTCCAG CGATGGAAAC GCAGAGACGC CTGTCAGCAA GTTCATGGAT 
CGGCTGCTCT CCTTGATGGT GTGTAACCAC GAAAAAGTGG GGCTGCAGAT ACGAAGCAAT 
GTCAAGGACT TGTTGGGTCT TGAATTGAGT CCTGCCCTTT ATCCAATGCT GTTTAACAAA 
TTAAAAAATA CCATCAGCAA GTTTTTTGAT CCTCAAGGAC AGGTTTTATT GACTGACACC 
AACACCCAGT TTGTGGAGCA GACCATAGCC ATAATGAAGA ACTTGCTTGA TAATCATGCT 
GAGGGTAGTT CTGAACACAT GGGACAAGCT AGCATCGAAA CTATGATGTT AAATCTGGTC 
AGATATGTTA GAGTGTTTGG GAATTTGGTG CAGGCAATTC AGATCAAAAC TAAGCTGTGC 
CAGCTGGTTC AAGTAATGAT GGAGAGGAGA GATGACCTTT CATTTTGCCA AGAGATGAAA 
TTTAGGAATA AAATGGTAGA ATATCTGACA GATTGGGTCA TGGGAACATC TAACCAAGCT 
GCAGATGAAG ATGTGAAATG TCTAACTAGA GATTTGGACC AGGCAAGCAT GGAAGCAGTG 
GTGTCATTGC TCGCTGGGCT CCCTCTGCAG CCTGAGGAAG GCGATGGGGT GGAATTGATG 
GAAGCCAAAT CCCAGCTGTT CCTTAAGTAT TTCACCCTCT TTATGAACCT TCTTAATGAC 
TGCAGTGAGG TCGAAGAAGA AAATGTCCAG ATTGGGGGCA GGAAGCGCGG CATGTCGCGA 
AGGCTGGCGT CCCTGCGCCA CTGCACCGTG CTGGCAATGT CCAACCTGCT CAATGCGAAT 
GTAGACAGCG GCCTGATGCA CTCTATAGTG CTAGGATTGG GTTACCACAA AGATCTTCAG 
ACAAGAGCCA CCTTCATGGA AGTCCTCACA AAAATCCTTC AGCAAGGCAC AGAGTTTGAT 
ACGCTCGCAG AAACCGTGTT AGCTGATCGA TTTGAGAGAC TTGTTGAGTT GGTCACGATG 
ATGGGTGATC AAGGAGAGCT CCCCATAGCC ATGGCTCTGG CCAACGTGGT GCCTTGTTCT 
CAGTGGGATG AACTTGCTCG AGTCCTTGTT ACTCTCTTTG ATTCCCGACA CTTACTGTAT 
CAGCTCCTTT GGAACATGTT TTCTAAGGAA GTTGAGCTGG CAGACTCCAT GCAGACACTT 
TTTAGAGGAA ACAGCTTGGC CAGTAAAATC ATGACCTTCT GCTTTAAGGT CTACGGTGCT 
ACATACCTGC AGAAGCTTCT GGAGCCTTTA CTCCGCTTTG TGATCACATC CTCCGAATGG 
CAGCACGTCA GCTTCGAGGT CGATCCCACC AGGTTAGAGC ATACTGAGAG CCTCGAGGAC 
AACCAGCGGA ACCTTTTGCA AATGACTGAG AAGTTCTTCC ACGCCATCAT CAGTTCTTCC 
TCCGAGTTCC CCCCTCAGCT ACGCAGTGTG TGCCACTGCC TGTACCAGGC AACTTGCCAC 
TCTCTACTGA ATAAAGCTAC CGTAAAAGAA AAAAAGGAAA ACAAAAAATC AGTTGTTAGC 
CAGAGATTCC CTCAGAACAG CATTGGCGCC GTGGGGAGTG CCATGTTCCT GAGATTCATC 
AACCCTGCCG TTGTCTCTCC CTACGAGGCA GGGATTTTAG AGAGAAAACC GCTCCCTAGA 
ATTGAAAGGG GGTTGAAGCT GATGTCAAAG ATTCTTCAGA GCATTGCCAA TCATGTCCTT 
TTCACAAAAG AAGAGTATAT GAGGCCTTTT AACGATTTTG TGAAAAGCAA CTTTGATGTA 
GCACGGAGAT TTTTCCTTGA TATCGCATCT GATTGTCCTG TAAGTGATGC CATAAACCAC 
AGCCTTTCCT TCATCAGCGA TGGCAATGTC TTGGCTTTGC ACCGTCTGCT CTGGAACAAC 
CAAGAGAAAA TCGGCCAATA TCTTTCAAGC AACAGAGATC ACAAAGCTGT TGGAAGGCGA 
CCTTTCGATA AGATGGCAAC ACTGCTTGCC TACCTGGGAC CTCCAGAGCA CAAGCCTGTG 
GCTGACACAC ACTGGTCCAG CCTAAATCTT ACCAGCTCCA AGTTTGAGGA ATTCATGACA 
AGGCACCAGG TACATGAAAA AGAGGAATTC AAGGCTCTGA AAACATTAAA TATCTTCTAT 
CAAGCAGGCA CTTCCAAAGC TGGAAATCCT GTTTTTTATT ATGTCACACG AAGGTTCAAG 
ACTGGCCAGA TCAACGGGGA TTTGCTTATA TACCACGTCT TACTAACTTT AAAGCCATAT 
TATGCAAAGC CATATGAGAT TGTAATGGAC CTCACTCATG CCGGGCCTAG CAATCGCTTT 
AAGACAGACT TCCTCTCCAA GTGGTTTGTT GTTTTCCCGG GCTTTGCTTA CGAGAACGTC 
TCTTCGGTCT ACATCTACAA CTGTAATTCT TGGGTTCGGG AGTACACCAA ATACCATGAG 
AGACTGCTCA CGGGACTCAA AGGCAGCAAA AGACTCTTTT TCATAGACTC TCCCTCAAAA 
CTGGCTGAGC ATATCGATTA TGATCAGCAA AAACTGCCAG CCGCGACCTT GGCTCTGGAT 
GAAGACCTCA AGGTGTACAA CAGTGCTCTC AAGCTGGCTC ACAAGGACAC CAAGGTGACC 
CTCAAGGTTG GCTCTACTGC GATCCAAGTG ACGTCATCTG AGCGAACTAA AGTCCTGGGA 
CAGACGGTTT TTCTGAATGA TATTTACTAT GCCTCTGAGA TCGAAGAAAT CTGTCTTGTG 
GATGAAAACC AGTTCACTCT GACCATTTCC AATCAGGGCA CTCCCCTCAC CTTCATGCAT 
CAAGAGTGTG AAGGCATTGT GGCGTCCATC ATTCACATCC GAACTCGATG GGAGCTGTCC 
CAGCCAGATT CTATCCCCCA GCACACTAAG ATCCGGCCAA AGGACGTTCC CGGAACACTT 
TTGAATATCG CATTACTTAA CTTGGGTAGT TCGGACCCAA GTTTACGTAA AACCCTGGCA 
GCTAATGAGC CACACCTCAC CTTAGAGTTT TTGGAAGAGT GCATTTCCGG ATTTAGCAAA 
TCCAGTATTG AACTGAAGCA CCTGTGTCTA GAATACATGA CTCCCTGGCT ATCAAATCTA 
GTCCGTTTCT GTAAACATAA TGATGATGCC AAACGACAGA GAGTCACTGC TATTCTTGAT 
AAACTTATAA CAATGACCAT AAATGAAAAG CAGATGTACC CATCGATCCA AGCCAAGATA 
TGGGGAAGCC TTGGGCAGAT TACAGATCTG CTCGATGTAG TGCTGGACAG CTTTATCAAA 
ACCAGTGCCA CAGGTGGTTT AGGATCCATC AAGGCTGAAG TGATGGCTGA CACGGCCGTG 
GCTCTGGCTT CTGGGAACGT CAAACTAGTT TCGAGCAAGG TGATTGGAAG AATGTGCAAA 
ATCATTGACA AGACCTGCCT CTCTCCAACT CCTACCTTAG AGCAGCACCT CATGTGGGAT 
GATATCGCCA TTTTAGCACG CTACATGCTA ATGCTGTCCT TCAATAATTC TCTGGATGTG 
GCGGCTCATC TTCCCTACCT CTTCCATGTG GTGACTCTGT TGGTGGCCAC AGGTCCCTTG 
TCCCTTCGTG CTTCCACACA CGGACTGGTC ATCAATATCA TCCATTCTCT GTGCACGTGC 
TCACAGCTTC ACTTTAGTGA AGAGACCAAG CAAGTTTTAC GACTGAGTCT GACTGAGTTC 
TCACTACCTA AGTTTTATTT GCTGTTCGGC ATCAGCAAAG TCAAATCAGC TGCCGTGATA 
GCCTTCCGGT CCAGTTACCG GGACAGGTCT TTCTCCCCTG GCTCCTATGA GAGGGAGACC 
TTTGCCCTGG CCTCCTTGGA AACAGTCACA GAAGCTCTCT TGGAGATCAT GGAGGCTTGT 
ATGAGAGATA TTCCTGCATG CAAGTGGCTG GACCAGTGGA CAGAGCTAGC CCAGAGGTTT 
GCGTTCCAGT ACAATCCCTC CCTGCAGCCA CGAGCTCTGG TTGTGTTTGG TTGTATCAGC 
AAAAGAGTGT CCCATGGGCA GATCAAGCAG ATTATCCGCA TTCTTAGCAA GGGACTTGAG 
AGCTGCCTAA AAGGACCTGA TAATTATAAC AGTCAAGTTC TGATTGAAGC TACCGTGATA 
GCTCTAACCA AATTACAACC ACTTCTTAAT AAGCAGGAGT CCCCTCTGCA CCGAGCTCTC 
TTCTGGGTGG CCGTTGCCGT GCTGCAGCTG GATGAAGTGG ACCTGTACTC GGCGGGCACT 
GCCCTCCTCG AACAGAACCT GCACACCTTA GACAGCCTCC TGCACATCTT CAACGACCAG 
AGCCCAGAAG AAGTGTTCAT GGAGATCCGG CAGCCTCTGG AGTGGCACTG CAAGCAGATG 
GACCACTTTG TGGGGCTCAA CTTTAACTCC AGCTTTAACT TTGCCCTGGT CGGGCACCTG 
CTGAAGGGAT TTCGGCATCC TTCACCTGTC ACTGTTGCAA GAACTGTGAG GATTTTGCAC 
ACCCTACTAG CATTGGTTAA CAAGCATAGG AATTGTGACA AGTTTGAGGT GAACACCCAG 
AGTGTGGCTT ACTTAGCAGC TTTACTTACT GTGTCTGAGG AGGTTCGAAG TCGCTGCAGC 
TTAAAGCATA GGAAATCACT CCTACTAACT GATATCACAA TGGAAAATGT GCCTATGGAT 
ATATATCCCA TCTATCACAG TGACCCCAGC CTTAGGACAC TAAAAGAAAC ACAACCGTGG 
TCCTCTCCAA AGGGATCTGA GAGACATCTT GCAGCCAGCT ACCCTCCTGT GGGCCAAACT 
AGCCCCCGAA CCAGAAAATC CATGAGCCTG GATATGGGGC AGCCTTCCCA GGCCAACACA 
AAGAAGTTGC TTGGGACAAG GAAAAGCTTC GATCATTTGA TCTCTGATGC CAAAGCTCCC 
AAAAGGCAAG AACAGGAGTC TGGAGTCACC ACGCCCCCCA AGATGCGGAG AGTGGCCGAG 
AACGACTACG AGATGGAAAC CCAGAGAATC TCCCCTCAGC CACACCCGCC TCTGCGGAAA 
GTGTCCGTGT CCGAATCCAA TGTGCTGCTG GACGAGGAAG TGCTCACAGA CCCCAAAACC 
CAGGCTCTTC TTCTCACTGT CCTGGCTACT CTGGTGAAGT ACACTGCGGA CGAGTTTGAC 
CAGCGCATTC TCTATGAGTA CTTAGCGGAG GCCAGTGTTG TCTTCCCAAA GGTTTTTCCA 
GTGGTACATA ATTTGTTGGA CTCAAAGATA AATACTCTGC TCTCATTATG CCAAGACCCA 
AATTTGCTAA ACCCGGTTCA TGGAATTGTG CAGAGTGTGG TCTACCACGA AGAGGCCCCG 
CCGCAGTACC AGACGGCGTA CCTCCAGAGT TTCGGTTTTA ATGGTCTGTG GAGGTTTGCA 
GGACCATTTT CTAAGCAGAC GCAGATCCCA GACTATGCCG AGCTCATCGT GAAGTTTCTC 
GATGCCTTGA TTGACACGTA TCTGCCTGGA ATTGATGAGG AAGCCAGTGA GGAGTCCCTC 
CTGACACCCA CCTCTCCCTA CCCGCCCGCG GTGCAGAGCC AGCTTAGCAT CACTGCGAAC 
CTCAACCTCT CTAACTCCAT GACCTCACTG GCAACTTCCC AGCATTCCCC AGGCATCGAC 
AAGGAGAATG TGGAGCTCTC GCCAACCACC GGGCACTGCA GCGGCGGGAG GACGCGCCAC 
GGCTCCGCCA GCCAGGTCCA GAAGCAAAGG AGTGCCGGCA GCTTCAAGAG GCACAGCATC 
AAGAAGATCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967653.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823513.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X14, mRNA.

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CloneID	OSa234701
Clone ID Related Accession (Same CDS sequence)	XM_031967653.1
Accession Version	XM_031967653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGCAGCCA AAGGACACGA TGAGGTTAGA TGAAACAATG 
CTGGTCAAAC AGTTGCTGCC GGAAATCTGC CATTTCCTTC ACACCTACCG GGAAGGGAAC 
CAGCACGCAG CCGAGCTTCG GAGCTCCGCC TCTGGCGTCC TTTTCTCCCT CAGCTGTAAT 
AACTTCAATG CCGTCTTCAG CCGGATTTCC ACCAGGTTAC AGGAATTGAC TGTTTGCTCA 
GAAGATAATG TTGATGTCCA TGATATAGAA TTATTACAAT ATATCAATGT GGATTGTGCA 
AAACTAAAGC GGCTCTTGCA GGAAACTGTA TTTAAGTTTA AAGCCTTAAA GAAGGTAGCT 
CAACTAGCAG TTATAAATAG CCTAGAAAAG GCATTTTGGA ATTGGGTAGA GAACTATCCT 
GATGAATTTA CAAAGCTGTA CCAGACTCCC CAGACAGATA TGGCGGAATG TGCAGAAAAA 
TTATTTGACT TGGTGGATGG TTTTGCTGAA AGCACCAAAC GTAAAGCTGC AGTTTGGCCA 
CTGCAAATCA TTCTTCTTGT CTTGTGTCCA GAAATAATCC AGGACATTTC CAAGGATGTG 
GTTGATGAAG CGAAAATGAA TAAGAAAATG TTTCTCGACA ATCTCAGAAG AGCGCTGGCC 
GGCCACAGTG GGAGCAGGCA GCTGACAGAG AGTGCCGCGA TCGCTTGTGT TAAACTGTGC 
AAAGCCAGCA CCTACATCAA CTGGGAGGAT AACTCGGTCA TTTTCCTGCT CGTCCAATCC 
ATGGTGGTGG ATCTGAAGAA CTTGCTCTTT AATCCCAGTA AGCCCTTCTC CAGAGGCAGC 
CAGAATGCTG ATGTGGACCT CATGATAGAC TGCCTGGTTT CTTGCTTTCG CATCAGCCCT 
CACAACAACC AACACTTTAA GATCTGTTTG GCCCAGAACT CCCCATCTAC ATTTCATTAT 
GTGCTGGTAA ACTCCCTCCA TCGAATTATC ACCAATTCCG CCCTGGATTG GTGGCCCAAG 
ATTGATGCTG TGTATTGCCA TTCTGTAGAA CTTCGAAACA TGTTTGGTGA AACGCTTCAC 
AAGGCCGTGC AGGGCTGTGG GCCTCACCCC GCGATACGAA TGGCGCCGAG TCTTACATTT 
AAAGAAAAAA TGACAAGCCT TAAATTTAAA GAAAAACCTA CGGACTTGGA AACAAGAAGT 
TATAAATACC TCTTATTATC CATGGTGAAA CTAATCCATG CAGATCCAAA GCTGTTGCTA 
TGTAATCCAA GAAAACAAGG GCCTGAAACT CAGGGGAGTA CAGCAGAGTT AATCACAGGC 
CTTGTGCAGC TCGTCCCACA GTCCAGCATG CCAGAAATAG CTCAAGAAGC AATGGAGGCT 
TTGCTGGTTC TTCATCAGTT GGATAGTATT GACTTATGGA ATCCAGATTC ACCTATAGAA 
ACATTCTGGG AGATTAGCTC ACAAATGCTT TTTTATATCT GTAAAAAGCT AACTACCCAT 
CAGATGCTTA GCAGTACAGA AATTCTCAAG TGGTTGCGAG AAATTTTGAT CTGTAGGAAC 
AAATATCTTC TTAAAAATAA GCAGTCAGAT AGGAGTTCTT CCCATTTCCT CTACCTTTAT 
GGGGCAGGCT ATGATGTCTC TTCTGGTGGA GGTACCAGTC ATATCTCTCT AGACCACGAG 
GAGTTTATGC GTACCTGTGC TCATGGACCA TCTCTCAGGA AAGTGAAAGG AAGCTCCTCA 
ATGGACGGTG CAATCGGGTC CAGCGGGACG CCCATCTGCC GCCAGGCCCA GACTAAGCTG 
GAGGTTGCTC TGTACATGTT TCTGTGGAGT CCCGACATTG AAGCCGTGCT CGTGGCCATG 
TCCTGCTTTC GACACCTGTG TGAGGAAGCC GACATCAGGT GTGGCATAGA TGAGATCTCG 
GTGCACCATT TCTTGCCAAA CTATAACACC TTCATGGAGT TCGCATCTGT CAGCAACATG 
ATGTCGACAG GAAGAGCAGC TCTTCAGAAA AGAGTGATGG CCCTGCTCAG GCGCATCGAA 
CACCCCACCG CTGGAAACAC CGAGGCTTGG GAAGATACTT ACGCCAAATG GGAGCAAGCT 
ACAAAATCAA TCCTCAATTA TCCGAAAGCC AAAATTGAAG ATGGCCAGGT TGCTGAGAGT 
CTTCACAAGA CCATTGTGAA GAGAAGAATG TCTCACGTGA GTGGAGGGGG CTCCATAGAC 
TTGTGCGACA CTGACTCGCT ACAGGAGTGG ATCAACATGA CTGGGTTTCT CTGTGCCCTT 
GGTGGCGTGT GCCTACAGCA GCGGAGCAAT GCCGGCTTTG CCACCTACAG CCCTCCCATG 
GGCCCTATCA GTGAACGCAA GGGCTCTCTC ATTTCCATGG TGTCCAGCGA TGGAAACGCA 
GAGACGCCTG TCAGCAAGTT CATGGATCGG CTGCTCTCCT TGATGGTGTG TAACCACGAA 
AAAGTGGGGC TGCAGATACG AAGCAATGTC AAGGACTTGT TGGGTCTTGA ATTGAGTCCT 
GCCCTTTATC CAATGCTGTT TAACAAATTA AAAAATACCA TCAGCAAGTT TTTTGATCCT 
CAAGGACAGG TTTTATTGAC TGACACCAAC ACCCAGTTTG TGGAGCAGAC CATAGCCATA 
ATGAAGAACT TGCTTGATAA TCATGCTGAG GGTAGTTCTG AACACATGGG ACAAGCTAGC 
ATCGAAACTA TGATGTTAAA TCTGGTCAGA TATGTTAGAG TGTTTGGGAA TTTGGTGCAG 
GCAATTCAGA TCAAAACTAA GCTGTGCCAG CTGGTTCAAG TAATGATGGA GAGGAGAGAT 
GACCTTTCAT TTTGCCAAGA GATGAAATTT AGGAATAAAA TGGTAGAATA TCTGACAGAT 
TGGGTCATGG GAACATCTAA CCAAGCTGCA GATGAAGATG TGAAATGTCT AACTAGAGAT 
TTGGACCAGG CAAGCATGGA AGCAGTGGTG TCATTGCTCG CTGGGCTCCC TCTGCAGCCT 
GAGGAAGGCG ATGGGGTGGA ATTGATGGAA GCCAAATCCC AGCTGTTCCT TAAGTATTTC 
ACCCTCTTTA TGAACCTTCT TAATGACTGC AGTGAGGTCG AAGAAGAAAA TGTCCAGATT 
GGGGGCAGGA AGCGCGGCAT GTCGCGAAGG CTGGCGTCCC TGCGCCACTG CACCGTGCTG 
GCAATGTCCA ACCTGCTCAA TGCGAATGTA GACAGCGGCC TGATGCACTC TATAGTGCTA 
GGATTGGGTT ACCACAAAGA TCTTCAGACA AGAGCCACCT TCATGGAAGT CCTCACAAAA 
ATCCTTCAGC AAGGCACAGA GTTTGATACG CTCGCAGAAA CCGTGTTAGC TGATCGATTT 
GAGAGACTTG TTGAGTTGGT CACGATGATG GGTGATCAAG GAGAGCTCCC CATAGCCATG 
GCTCTGGCCA ACGTGGTGCC TTGTTCTCAG TGGGATGAAC TTGCTCGAGT CCTTGTTACT 
CTCTTTGATT CCCGACACTT ACTGTATCAG CTCCTTTGGA ACATGTTTTC TAAGGAAGTT 
GAGCTGGCAG ACTCCATGCA GACACTTTTT AGAGGAAACA GCTTGGCCAG TAAAATCATG 
ACCTTCTGCT TTAAGGTCTA CGGTGCTACA TACCTGCAGA AGCTTCTGGA GCCTTTACTC 
CGCTTTGTGA TCACATCCTC CGAATGGCAG CACGTCAGCT TCGAGGTCGA TCCCACCAGG 
TTAGAGCATA CTGAGAGCCT CGAGGACAAC CAGCGGAACC TTTTGCAAAT GACTGAGAAG 
TTCTTCCACG CCATCATCAG TTCTTCCTCC GAGTTCCCCC CTCAGCTACG CAGTGTGTGC 
CACTGCCTGT ACCAGGCAAC TTGCCACTCT CTACTGAATA AAGCTACCGT AAAAGAAAAA 
AAGGAAAACA AAAAATCAGT TGTTAGCCAG AGATTCCCTC AGAACAGCAT TGGCGCCGTG 
GGGAGTGCCA TGTTCCTGAG ATTCATCAAC CCTGCCGTTG TCTCTCCCTA CGAGGCAGGG 
ATTTTAGAGA GAAAACCGCT CCCTAGAATT GAAAGGGGGT TGAAGCTGAT GTCAAAGATT 
CTTCAGAGCA TTGCCAATCA TGTCCTTTTC ACAAAAGAAG AGTATATGAG GCCTTTTAAC 
GATTTTGTGA AAAGCAACTT TGATGTAGCA CGGAGATTTT TCCTTGATAT CGCATCTGAT 
TGTCCTGTAA GTGATGCCAT AAACCACAGC CTTTCCTTCA TCAGCGATGG CAATGTCTTG 
GCTTTGCACC GTCTGCTCTG GAACAACCAA GAGAAAATCG GCCAATATCT TTCAAGCAAC 
AGAGATCACA AAGCTGTTGG AAGGCGACCT TTCGATAAGA TGGCAACACT GCTTGCCTAC 
CTGGGACCTC CAGAGCACAA GCCTGTGGCT GACACACACT GGTCCAGCCT AAATCTTACC 
AGCTCCAAGT TTGAGGAATT CATGACAAGG CACCAGGTAC ATGAAAAAGA GGAATTCAAG 
GCTCTGAAAA CATTAAATAT CTTCTATCAA GCAGGCACTT CCAAAGCTGG AAATCCTGTT 
TTTTATTATG TCACACGAAG GTTCAAGACT GGCCAGATCA ACGGGGATTT GCTTATATAC 
CACGTCTTAC TAACTTTAAA GCCATATTAT GCAAAGCCAT ATGAGATTGT AATGGACCTC 
ACTCATGCCG GGCCTAGCAA TCGCTTTAAG ACAGACTTCC TCTCCAAGTG GTTTGTTGTT 
TTCCCGGGCT TTGCTTACGA GAACGTCTCT TCGGTCTACA TCTACAACTG TAATTCTTGG 
GTTCGGGAGT ACACCAAATA CCATGAGAGA CTGCTCACGG GACTCAAAGG CAGCAAAAGA 
CTCTTTTTCA TAGACTCTCC CTCAAAACTG GCTGAGCATA TCGATTATGA TCAGCAAAAA 
CTGCCAGCCG CGACCTTGGC TCTGGATGAA GACCTCAAGG TGTACAACAG TGCTCTCAAG 
CTGGCTCACA AGGACACCAA GGTGACCCTC AAGGTTGGCT CTACTGCGAT CCAAGTGACG 
TCATCTGAGC GAACTAAAGT CCTGGGACAG ACGGTTTTTC TGAATGATAT TTACTATGCC 
TCTGAGATCG AAGAAATCTG TCTTGTGGAT GAAAACCAGT TCACTCTGAC CATTTCCAAT 
CAGGGCACTC CCCTCACCTT CATGCATCAA GAGTGTGAAG GCATTGTGGC GTCCATCATT 
CACATCCGAA CTCGATGGGA GCTGTCCCAG CCAGATTCTA TCCCCCAGCA CACTAAGATC 
CGGCCAAAGG ACGTTCCCGG AACACTTTTG AATATCGCAT TACTTAACTT GGGTAGTTCG 
GACCCAAGTT TACGGTCAGC CGCCTATAAT CTTCTCTGTG CCTTAACCTG TACCTTTAAT 
TTAAAAATTG AGGGCCAATT ACTAGAAACA TCAGGTCTGT GTATACCTGC CAACAATACC 
CTTTTTATTG TCTCTATTAG TAAAACCCTG GCAGCTAATG AGCCACACCT CACCTTAGAG 
TTTTTGGAAG AGTGCATTTC CGGATTTAGC AAATCCAGTA TTGAACTGAA GCACCTGTGT 
CTAGAATACA TGACTCCCTG GCTATCAAAT CTAGTCCGTT TCTGTAAACA TAATGATGAT 
GCCAAACGAC AGAGAGTCAC TGCTATTCTT GATAAACTTA TAACAATGAC CATAAATGAA 
AAGCAGATGT ACCCATCGAT CCAAGCCAAG ATATGGGGAA GCCTTGGGCA GATTACAGAT 
CTGCTCGATG TAGTGCTGGA CAGCTTTATC AAAACCAGTG CCACAGGTGG TTTAGGATCC 
ATCAAGGCTG AAGTGATGGC TGACACGGCC GTGGCTCTGG CTTCTGGGAA CGTCAAACTA 
GTTTCGAGCA AGGTGATTGG AAGAATGTGC AAAATCATTG ACAAGACCTG CCTCTCTCCA 
ACTCCTACCT TAGAGCAGCA CCTCATGTGG GATGATATCG CCATTTTAGC ACGCTACATG 
CTAATGCTGT CCTTCAATAA TTCTCTGGAT GTGGCGGCTC ATCTTCCCTA CCTCTTCCAT 
GTGGTGACTC TGTTGGTGGC CACAGGTCCC TTGTCCCTTC GTGCTTCCAC ACACGGACTG 
GTCATCAATA TCATCCATTC TCTGTGCACG TGCTCACAGC TTCACTTTAG TGAAGAGACC 
AAGCAAGTTT TACGACTGAG TCTGACTGAG TTCTCACTAC CTAAGTTTTA TTTGCTGTTC 
GGCATCAGCA AAGTCAAATC AGCTGCCGTG ATAGCCTTCC GGTCCAGTTA CCGGGACAGG 
TCTTTCTCCC CTGGCTCCTA TGAGAGGGAG ACCTTTGCCC TGGCCTCCTT GGAAACAGTC 
ACAGAAGCTC TCTTGGAGAT CATGGAGGCT TGTATGAGAG ATATTCCTGC ATGCAAGTGG 
CTGGACCAGT GGACAGAGCT AGCCCAGAGG TTTGCGTTCC AGTACAATCC CTCCCTGCAG 
CCACGAGCTC TGGTTGTGTT TGGTTGTATC AGCAAAAGAG TGTCCCATGG GCAGATCAAG 
CAGATTATCC GCATTCTTAG CAAGGGACTT GAGAGCTGCC TAAAAGGACC TGATAATTAT 
AACAGTCAAG TTCTGATTGA AGCTACCGTG ATAGCTCTAA CCAAATTACA ACCACTTCTT 
AATAAGCAGG AGTCCCCTCT GCACCGAGCT CTCTTCTGGG TGGCCGTTGC CGTGCTGCAG 
CTGGATGAAG TGGACCTGTA CTCGGCGGGC ACTGCCCTCC TCGAACAGAA CCTGCACACC 
TTAGACAGCC TCCTGCACAT CTTCAACGAC CAGAGCCCAG AAGAAGTGTT CATGGAGATC 
CGGCAGCCTC TGGAGTGGCA CTGCAAGCAG ATGGACCACT TTGTGGGGCT CAACTTTAAC 
TCCAGCTTTA ACTTTGCCCT GGTCGGGCAC CTGCTGAAGG GATTTCGGCA TCCTTCACCT 
GTCACTGTTG CAAGAACTGT GAGGATTTTG CACACCCTAC TAGCATTGGT TAACAAGCAT 
AGGAATTGTG ACAAGTTTGA GGTGAACACC CAGAGTGTGG CTTACTTAGC AGCTTTACTT 
ACTGTGTCTG AGGAGGTTCG AAGTCGCTGC AGCTTAAAGC ATAGGAAATC ACTCCTACTA 
ACTGATATCA CAATGGAAAA TGTGCCTATG GATATATATC CCATCTATCA CAGTGACCCC 
AGCCTTAGGA CACTAAAAGA AACACAACCG TGGTCCTCTC CAAAGGGATC TGAGAGACAT 
CTTGCAGCCA GCTACCCTCC TGTGGGCCAA ACTAGCCCCC GAACCAGAAA ATCCATGAGC 
CTGGATATGG GGCAGCCTTC CCAGGCCAAC ACAAAGAAGT TGCTTGGGAC AAGGAAAAGC 
TTCGATCATT TGATCTCTGA TGCCAAAGCT CCCAAAAGGC AAGAACAGGA GTCTGGAGTC 
ACCACGCCCC CCAAGATGCG GAGAGTGGCC GAGAACGACT ACGAGATGGA AACCCAGAGA 
ATCTCCCCTC AGCCACACCC GCCTCTGCGG AAAGTGTCCG TGTCCGAATC CAATGTGCTG 
CTGGACGAGG AAGTGCTCAC AGACCCCAAA ACCCAGGCTC TTCTTCTCAC TGTCCTGGCT 
ACTCTGGTGA AGTACACTGC GGACGAGTTT GACCAGCGCA TTCTCTATGA GTACTTAGCG 
GAGGCCAGTG TTGTCTTCCC AAAGGTTTTT CCAGTGGTAC ATAATTTGTT GGACTCAAAG 
ATAAATACTC TGCTCTCATT ATGCCAAGAC CCAAATTTGC TAAACCCGGT TCATGGAATT 
GTGCAGAGTG TGGTCTACCA CGAAGAGGCC CCGCCGCAGT ACCAGACGGC GTACCTCCAG 
AGTTTCGGTT TTAATGGTCT GTGGAGGTTT GCAGGACCAT TTTCTAAGCA GACGCAGATC 
CCAGACTATG CCGAGCTCAT CGTGAAGTTT CTCGATGCCT TGATTGACAC GTATCTGCCT 
GGAATTGATG AGGAAGCCAG TGAGGAGTCC CTCCTGACAC CCACCTCTCC CTACCCGCCC 
GCGGTGCAGA GCCAGCTTAG CATCACTGCG AACCTCAACC TCTCTAACTC CATGACCTCA 
CTGGCAACTT CCCAGCATTC CCCAGGCATC GACAAGGAGA ATGTGGAGCT CTCGCCAACC 
ACCGGGCACT GCAGCGGCGG GAGGACGCGC CACGGCTCCG CCAGCCAGGT CCAGAAGCAA 
AGGAGTGCCG GCAGCTTCAA GAGGCACAGC ATCAAGAAGA TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823513.1
CDS	9..8516
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIVLGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKQESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967653.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967653.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGCAGCCA AAGGACACGA TGAGGTTAGA TGAAACAATG 
CTGGTCAAAC AGTTGCTGCC GGAAATCTGC CATTTCCTTC ACACCTACCG GGAAGGGAAC 
CAGCACGCAG CCGAGCTTCG GAGCTCCGCC TCTGGCGTCC TTTTCTCCCT CAGCTGTAAT 
AACTTCAATG CCGTCTTCAG CCGGATTTCC ACCAGGTTAC AGGAATTGAC TGTTTGCTCA 
GAAGATAATG TTGATGTCCA TGATATAGAA TTATTACAAT ATATCAATGT GGATTGTGCA 
AAACTAAAGC GGCTCTTGCA GGAAACTGTA TTTAAGTTTA AAGCCTTAAA GAAGGTAGCT 
CAACTAGCAG TTATAAATAG CCTAGAAAAG GCATTTTGGA ATTGGGTAGA GAACTATCCT 
GATGAATTTA CAAAGCTGTA CCAGACTCCC CAGACAGATA TGGCGGAATG TGCAGAAAAA 
TTATTTGACT TGGTGGATGG TTTTGCTGAA AGCACCAAAC GTAAAGCTGC AGTTTGGCCA 
CTGCAAATCA TTCTTCTTGT CTTGTGTCCA GAAATAATCC AGGACATTTC CAAGGATGTG 
GTTGATGAAG CGAAAATGAA TAAGAAAATG TTTCTCGACA ATCTCAGAAG AGCGCTGGCC 
GGCCACAGTG GGAGCAGGCA GCTGACAGAG AGTGCCGCGA TCGCTTGTGT TAAACTGTGC 
AAAGCCAGCA CCTACATCAA CTGGGAGGAT AACTCGGTCA TTTTCCTGCT CGTCCAATCC 
ATGGTGGTGG ATCTGAAGAA CTTGCTCTTT AATCCCAGTA AGCCCTTCTC CAGAGGCAGC 
CAGAATGCTG ATGTGGACCT CATGATAGAC TGCCTGGTTT CTTGCTTTCG CATCAGCCCT 
CACAACAACC AACACTTTAA GATCTGTTTG GCCCAGAACT CCCCATCTAC ATTTCATTAT 
GTGCTGGTAA ACTCCCTCCA TCGAATTATC ACCAATTCCG CCCTGGATTG GTGGCCCAAG 
ATTGATGCTG TGTATTGCCA TTCTGTAGAA CTTCGAAACA TGTTTGGTGA AACGCTTCAC 
AAGGCCGTGC AGGGCTGTGG GCCTCACCCC GCGATACGAA TGGCGCCGAG TCTTACATTT 
AAAGAAAAAA TGACAAGCCT TAAATTTAAA GAAAAACCTA CGGACTTGGA AACAAGAAGT 
TATAAATACC TCTTATTATC CATGGTGAAA CTAATCCATG CAGATCCAAA GCTGTTGCTA 
TGTAATCCAA GAAAACAAGG GCCTGAAACT CAGGGGAGTA CAGCAGAGTT AATCACAGGC 
CTTGTGCAGC TCGTCCCACA GTCCAGCATG CCAGAAATAG CTCAAGAAGC AATGGAGGCT 
TTGCTGGTTC TTCATCAGTT GGATAGTATT GACTTATGGA ATCCAGATTC ACCTATAGAA 
ACATTCTGGG AGATTAGCTC ACAAATGCTT TTTTATATCT GTAAAAAGCT AACTACCCAT 
CAGATGCTTA GCAGTACAGA AATTCTCAAG TGGTTGCGAG AAATTTTGAT CTGTAGGAAC 
AAATATCTTC TTAAAAATAA GCAGTCAGAT AGGAGTTCTT CCCATTTCCT CTACCTTTAT 
GGGGCAGGCT ATGATGTCTC TTCTGGTGGA GGTACCAGTC ATATCTCTCT AGACCACGAG 
GAGTTTATGC GTACCTGTGC TCATGGACCA TCTCTCAGGA AAGTGAAAGG AAGCTCCTCA 
ATGGACGGTG CAATCGGGTC CAGCGGGACG CCCATCTGCC GCCAGGCCCA GACTAAGCTG 
GAGGTTGCTC TGTACATGTT TCTGTGGAGT CCCGACATTG AAGCCGTGCT CGTGGCCATG 
TCCTGCTTTC GACACCTGTG TGAGGAAGCC GACATCAGGT GTGGCATAGA TGAGATCTCG 
GTGCACCATT TCTTGCCAAA CTATAACACC TTCATGGAGT TCGCATCTGT CAGCAACATG 
ATGTCGACAG GAAGAGCAGC TCTTCAGAAA AGAGTGATGG CCCTGCTCAG GCGCATCGAA 
CACCCCACCG CTGGAAACAC CGAGGCTTGG GAAGATACTT ACGCCAAATG GGAGCAAGCT 
ACAAAATCAA TCCTCAATTA TCCGAAAGCC AAAATTGAAG ATGGCCAGGT TGCTGAGAGT 
CTTCACAAGA CCATTGTGAA GAGAAGAATG TCTCACGTGA GTGGAGGGGG CTCCATAGAC 
TTGTGCGACA CTGACTCGCT ACAGGAGTGG ATCAACATGA CTGGGTTTCT CTGTGCCCTT 
GGTGGCGTGT GCCTACAGCA GCGGAGCAAT GCCGGCTTTG CCACCTACAG CCCTCCCATG 
GGCCCTATCA GTGAACGCAA GGGCTCTCTC ATTTCCATGG TGTCCAGCGA TGGAAACGCA 
GAGACGCCTG TCAGCAAGTT CATGGATCGG CTGCTCTCCT TGATGGTGTG TAACCACGAA 
AAAGTGGGGC TGCAGATACG AAGCAATGTC AAGGACTTGT TGGGTCTTGA ATTGAGTCCT 
GCCCTTTATC CAATGCTGTT TAACAAATTA AAAAATACCA TCAGCAAGTT TTTTGATCCT 
CAAGGACAGG TTTTATTGAC TGACACCAAC ACCCAGTTTG TGGAGCAGAC CATAGCCATA 
ATGAAGAACT TGCTTGATAA TCATGCTGAG GGTAGTTCTG AACACATGGG ACAAGCTAGC 
ATCGAAACTA TGATGTTAAA TCTGGTCAGA TATGTTAGAG TGTTTGGGAA TTTGGTGCAG 
GCAATTCAGA TCAAAACTAA GCTGTGCCAG CTGGTTCAAG TAATGATGGA GAGGAGAGAT 
GACCTTTCAT TTTGCCAAGA GATGAAATTT AGGAATAAAA TGGTAGAATA TCTGACAGAT 
TGGGTCATGG GAACATCTAA CCAAGCTGCA GATGAAGATG TGAAATGTCT AACTAGAGAT 
TTGGACCAGG CAAGCATGGA AGCAGTGGTG TCATTGCTCG CTGGGCTCCC TCTGCAGCCT 
GAGGAAGGCG ATGGGGTGGA ATTGATGGAA GCCAAATCCC AGCTGTTCCT TAAGTATTTC 
ACCCTCTTTA TGAACCTTCT TAATGACTGC AGTGAGGTCG AAGAAGAAAA TGTCCAGATT 
GGGGGCAGGA AGCGCGGCAT GTCGCGAAGG CTGGCGTCCC TGCGCCACTG CACCGTGCTG 
GCAATGTCCA ACCTGCTCAA TGCGAATGTA GACAGCGGCC TGATGCACTC TATAGTGCTA 
GGATTGGGTT ACCACAAAGA TCTTCAGACA AGAGCCACCT TCATGGAAGT CCTCACAAAA 
ATCCTTCAGC AAGGCACAGA GTTTGATACG CTCGCAGAAA CCGTGTTAGC TGATCGATTT 
GAGAGACTTG TTGAGTTGGT CACGATGATG GGTGATCAAG GAGAGCTCCC CATAGCCATG 
GCTCTGGCCA ACGTGGTGCC TTGTTCTCAG TGGGATGAAC TTGCTCGAGT CCTTGTTACT 
CTCTTTGATT CCCGACACTT ACTGTATCAG CTCCTTTGGA ACATGTTTTC TAAGGAAGTT 
GAGCTGGCAG ACTCCATGCA GACACTTTTT AGAGGAAACA GCTTGGCCAG TAAAATCATG 
ACCTTCTGCT TTAAGGTCTA CGGTGCTACA TACCTGCAGA AGCTTCTGGA GCCTTTACTC 
CGCTTTGTGA TCACATCCTC CGAATGGCAG CACGTCAGCT TCGAGGTCGA TCCCACCAGG 
TTAGAGCATA CTGAGAGCCT CGAGGACAAC CAGCGGAACC TTTTGCAAAT GACTGAGAAG 
TTCTTCCACG CCATCATCAG TTCTTCCTCC GAGTTCCCCC CTCAGCTACG CAGTGTGTGC 
CACTGCCTGT ACCAGGCAAC TTGCCACTCT CTACTGAATA AAGCTACCGT AAAAGAAAAA 
AAGGAAAACA AAAAATCAGT TGTTAGCCAG AGATTCCCTC AGAACAGCAT TGGCGCCGTG 
GGGAGTGCCA TGTTCCTGAG ATTCATCAAC CCTGCCGTTG TCTCTCCCTA CGAGGCAGGG 
ATTTTAGAGA GAAAACCGCT CCCTAGAATT GAAAGGGGGT TGAAGCTGAT GTCAAAGATT 
CTTCAGAGCA TTGCCAATCA TGTCCTTTTC ACAAAAGAAG AGTATATGAG GCCTTTTAAC 
GATTTTGTGA AAAGCAACTT TGATGTAGCA CGGAGATTTT TCCTTGATAT CGCATCTGAT 
TGTCCTGTAA GTGATGCCAT AAACCACAGC CTTTCCTTCA TCAGCGATGG CAATGTCTTG 
GCTTTGCACC GTCTGCTCTG GAACAACCAA GAGAAAATCG GCCAATATCT TTCAAGCAAC 
AGAGATCACA AAGCTGTTGG AAGGCGACCT TTCGATAAGA TGGCAACACT GCTTGCCTAC 
CTGGGACCTC CAGAGCACAA GCCTGTGGCT GACACACACT GGTCCAGCCT AAATCTTACC 
AGCTCCAAGT TTGAGGAATT CATGACAAGG CACCAGGTAC ATGAAAAAGA GGAATTCAAG 
GCTCTGAAAA CATTAAATAT CTTCTATCAA GCAGGCACTT CCAAAGCTGG AAATCCTGTT 
TTTTATTATG TCACACGAAG GTTCAAGACT GGCCAGATCA ACGGGGATTT GCTTATATAC 
CACGTCTTAC TAACTTTAAA GCCATATTAT GCAAAGCCAT ATGAGATTGT AATGGACCTC 
ACTCATGCCG GGCCTAGCAA TCGCTTTAAG ACAGACTTCC TCTCCAAGTG GTTTGTTGTT 
TTCCCGGGCT TTGCTTACGA GAACGTCTCT TCGGTCTACA TCTACAACTG TAATTCTTGG 
GTTCGGGAGT ACACCAAATA CCATGAGAGA CTGCTCACGG GACTCAAAGG CAGCAAAAGA 
CTCTTTTTCA TAGACTCTCC CTCAAAACTG GCTGAGCATA TCGATTATGA TCAGCAAAAA 
CTGCCAGCCG CGACCTTGGC TCTGGATGAA GACCTCAAGG TGTACAACAG TGCTCTCAAG 
CTGGCTCACA AGGACACCAA GGTGACCCTC AAGGTTGGCT CTACTGCGAT CCAAGTGACG 
TCATCTGAGC GAACTAAAGT CCTGGGACAG ACGGTTTTTC TGAATGATAT TTACTATGCC 
TCTGAGATCG AAGAAATCTG TCTTGTGGAT GAAAACCAGT TCACTCTGAC CATTTCCAAT 
CAGGGCACTC CCCTCACCTT CATGCATCAA GAGTGTGAAG GCATTGTGGC GTCCATCATT 
CACATCCGAA CTCGATGGGA GCTGTCCCAG CCAGATTCTA TCCCCCAGCA CACTAAGATC 
CGGCCAAAGG ACGTTCCCGG AACACTTTTG AATATCGCAT TACTTAACTT GGGTAGTTCG 
GACCCAAGTT TACGGTCAGC CGCCTATAAT CTTCTCTGTG CCTTAACCTG TACCTTTAAT 
TTAAAAATTG AGGGCCAATT ACTAGAAACA TCAGGTCTGT GTATACCTGC CAACAATACC 
CTTTTTATTG TCTCTATTAG TAAAACCCTG GCAGCTAATG AGCCACACCT CACCTTAGAG 
TTTTTGGAAG AGTGCATTTC CGGATTTAGC AAATCCAGTA TTGAACTGAA GCACCTGTGT 
CTAGAATACA TGACTCCCTG GCTATCAAAT CTAGTCCGTT TCTGTAAACA TAATGATGAT 
GCCAAACGAC AGAGAGTCAC TGCTATTCTT GATAAACTTA TAACAATGAC CATAAATGAA 
AAGCAGATGT ACCCATCGAT CCAAGCCAAG ATATGGGGAA GCCTTGGGCA GATTACAGAT 
CTGCTCGATG TAGTGCTGGA CAGCTTTATC AAAACCAGTG CCACAGGTGG TTTAGGATCC 
ATCAAGGCTG AAGTGATGGC TGACACGGCC GTGGCTCTGG CTTCTGGGAA CGTCAAACTA 
GTTTCGAGCA AGGTGATTGG AAGAATGTGC AAAATCATTG ACAAGACCTG CCTCTCTCCA 
ACTCCTACCT TAGAGCAGCA CCTCATGTGG GATGATATCG CCATTTTAGC ACGCTACATG 
CTAATGCTGT CCTTCAATAA TTCTCTGGAT GTGGCGGCTC ATCTTCCCTA CCTCTTCCAT 
GTGGTGACTC TGTTGGTGGC CACAGGTCCC TTGTCCCTTC GTGCTTCCAC ACACGGACTG 
GTCATCAATA TCATCCATTC TCTGTGCACG TGCTCACAGC TTCACTTTAG TGAAGAGACC 
AAGCAAGTTT TACGACTGAG TCTGACTGAG TTCTCACTAC CTAAGTTTTA TTTGCTGTTC 
GGCATCAGCA AAGTCAAATC AGCTGCCGTG ATAGCCTTCC GGTCCAGTTA CCGGGACAGG 
TCTTTCTCCC CTGGCTCCTA TGAGAGGGAG ACCTTTGCCC TGGCCTCCTT GGAAACAGTC 
ACAGAAGCTC TCTTGGAGAT CATGGAGGCT TGTATGAGAG ATATTCCTGC ATGCAAGTGG 
CTGGACCAGT GGACAGAGCT AGCCCAGAGG TTTGCGTTCC AGTACAATCC CTCCCTGCAG 
CCACGAGCTC TGGTTGTGTT TGGTTGTATC AGCAAAAGAG TGTCCCATGG GCAGATCAAG 
CAGATTATCC GCATTCTTAG CAAGGGACTT GAGAGCTGCC TAAAAGGACC TGATAATTAT 
AACAGTCAAG TTCTGATTGA AGCTACCGTG ATAGCTCTAA CCAAATTACA ACCACTTCTT 
AATAAGCAGG AGTCCCCTCT GCACCGAGCT CTCTTCTGGG TGGCCGTTGC CGTGCTGCAG 
CTGGATGAAG TGGACCTGTA CTCGGCGGGC ACTGCCCTCC TCGAACAGAA CCTGCACACC 
TTAGACAGCC TCCTGCACAT CTTCAACGAC CAGAGCCCAG AAGAAGTGTT CATGGAGATC 
CGGCAGCCTC TGGAGTGGCA CTGCAAGCAG ATGGACCACT TTGTGGGGCT CAACTTTAAC 
TCCAGCTTTA ACTTTGCCCT GGTCGGGCAC CTGCTGAAGG GATTTCGGCA TCCTTCACCT 
GTCACTGTTG CAAGAACTGT GAGGATTTTG CACACCCTAC TAGCATTGGT TAACAAGCAT 
AGGAATTGTG ACAAGTTTGA GGTGAACACC CAGAGTGTGG CTTACTTAGC AGCTTTACTT 
ACTGTGTCTG AGGAGGTTCG AAGTCGCTGC AGCTTAAAGC ATAGGAAATC ACTCCTACTA 
ACTGATATCA CAATGGAAAA TGTGCCTATG GATATATATC CCATCTATCA CAGTGACCCC 
AGCCTTAGGA CACTAAAAGA AACACAACCG TGGTCCTCTC CAAAGGGATC TGAGAGACAT 
CTTGCAGCCA GCTACCCTCC TGTGGGCCAA ACTAGCCCCC GAACCAGAAA ATCCATGAGC 
CTGGATATGG GGCAGCCTTC CCAGGCCAAC ACAAAGAAGT TGCTTGGGAC AAGGAAAAGC 
TTCGATCATT TGATCTCTGA TGCCAAAGCT CCCAAAAGGC AAGAACAGGA GTCTGGAGTC 
ACCACGCCCC CCAAGATGCG GAGAGTGGCC GAGAACGACT ACGAGATGGA AACCCAGAGA 
ATCTCCCCTC AGCCACACCC GCCTCTGCGG AAAGTGTCCG TGTCCGAATC CAATGTGCTG 
CTGGACGAGG AAGTGCTCAC AGACCCCAAA ACCCAGGCTC TTCTTCTCAC TGTCCTGGCT 
ACTCTGGTGA AGTACACTGC GGACGAGTTT GACCAGCGCA TTCTCTATGA GTACTTAGCG 
GAGGCCAGTG TTGTCTTCCC AAAGGTTTTT CCAGTGGTAC ATAATTTGTT GGACTCAAAG 
ATAAATACTC TGCTCTCATT ATGCCAAGAC CCAAATTTGC TAAACCCGGT TCATGGAATT 
GTGCAGAGTG TGGTCTACCA CGAAGAGGCC CCGCCGCAGT ACCAGACGGC GTACCTCCAG 
AGTTTCGGTT TTAATGGTCT GTGGAGGTTT GCAGGACCAT TTTCTAAGCA GACGCAGATC 
CCAGACTATG CCGAGCTCAT CGTGAAGTTT CTCGATGCCT TGATTGACAC GTATCTGCCT 
GGAATTGATG AGGAAGCCAG TGAGGAGTCC CTCCTGACAC CCACCTCTCC CTACCCGCCC 
GCGGTGCAGA GCCAGCTTAG CATCACTGCG AACCTCAACC TCTCTAACTC CATGACCTCA 
CTGGCAACTT CCCAGCATTC CCCAGGCATC GACAAGGAGA ATGTGGAGCT CTCGCCAACC 
ACCGGGCACT GCAGCGGCGG GAGGACGCGC CACGGCTCCG CCAGCCAGGT CCAGAAGCAA 
AGGAGTGCCG GCAGCTTCAA GAGGCACAGC ATCAAGAAGA TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967654.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823514.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X15, mRNA.

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CloneID	OSa234702
Clone ID Related Accession (Same CDS sequence)	XM_031967654.1
Accession Version	XM_031967654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGCAGCCA AAGGACACGA TGAGGTTAGA TGAAACAATG 
CTGGTCAAAC AGTTGCTGCC GGAAATCTGC CATTTCCTTC ACACCTACCG GGAAGGGAAC 
CAGCACGCAG CCGAGCTTCG GAGCTCCGCC TCTGGCGTCC TTTTCTCCCT CAGCTGTAAT 
AACTTCAATG CCGTCTTCAG CCGGATTTCC ACCAGGTTAC AGGAATTGAC TGTTTGCTCA 
GAAGATAATG TTGATGTCCA TGATATAGAA TTATTACAAT ATATCAATGT GGATTGTGCA 
AAACTAAAGC GGCTCTTGCA GGAAACTGTA TTTAAGTTTA AAGCCTTAAA GAAGGTAGCT 
CAACTAGCAG TTATAAATAG CCTAGAAAAG GCATTTTGGA ATTGGGTAGA GAACTATCCT 
GATGAATTTA CAAAGCTGTA CCAGACTCCC CAGACAGATA TGGCGGAATG TGCAGAAAAA 
TTATTTGACT TGGTGGATGG TTTTGCTGAA AGCACCAAAC GTAAAGCTGC AGTTTGGCCA 
CTGCAAATCA TTCTTCTTGT CTTGTGTCCA GAAATAATCC AGGACATTTC CAAGGATGTG 
GTTGATGAAG CGAAAATGAA TAAGAAAATG TTTCTCGACA ATCTCAGAAG AGCGCTGGCC 
GGCCACAGTG GGAGCAGGCA GCTGACAGAG AGTGCCGCGA TCGCTTGTGT TAAACTGTGC 
AAAGCCAGCA CCTACATCAA CTGGGAGGAT AACTCGGTCA TTTTCCTGCT CGTCCAATCC 
ATGGTGGTGG ATCTGAAGAA CTTGCTCTTT AATCCCAGTA AGCCCTTCTC CAGAGGCAGC 
CAGAATGCTG ATGTGGACCT CATGATAGAC TGCCTGGTTT CTTGCTTTCG CATCAGCCCT 
CACAACAACC AACACTTTAA GATCTGTTTG GCCCAGAACT CCCCATCTAC ATTTCATTAT 
GTGCTGGTAA ACTCCCTCCA TCGAATTATC ACCAATTCCG CCCTGGATTG GTGGCCCAAG 
ATTGATGCTG TGTATTGCCA TTCTGTAGAA CTTCGAAACA TGTTTGGTGA AACGCTTCAC 
AAGGCCGTGC AGGGCTGTGG GCCTCACCCC GCGATACGAA TGGCGCCGAG TCTTACATTT 
AAAGAAAAAA TGACAAGCCT TAAATTTAAA GAAAAACCTA CGGACTTGGA AACAAGAAGT 
TATAAATACC TCTTATTATC CATGGTGAAA CTAATCCATG CAGATCCAAA GCTGTTGCTA 
TGTAATCCAA GAAAACAAGG GCCTGAAACT CAGGGGAGTA CAGCAGAGTT AATCACAGGC 
CTTGTGCAGC TCGTCCCACA GTCCAGCATG CCAGAAATAG CTCAAGAAGC AATGGAGGCT 
TTGCTGGTTC TTCATCAGTT GGATAGTATT GACTTATGGA ATCCAGATTC ACCTATAGAA 
ACATTCTGGG AGATTAGCTC ACAAATGCTT TTTTATATCT GTAAAAAGCT AACTACCCAT 
CAGATGCTTA GCAGTACAGA AATTCTCAAG TGGTTGCGAG AAATTTTGAT CTGTAGGAAC 
AAATATCTTC TTAAAAATAA GCAGTCAGAT AGGAGTTCTT CCCATTTCCT CTACCTTTAT 
GGGGCAGGCT ATGATGTCTC TTCTGGTGGA GGTACCAGTC ATATCTCTCT AGACCACGAG 
GAGTTTATGC GTACCTGTGC TCATGGACCA TCTCTCAGGA AAGTGAAAGG AAGCTCCTCA 
ATGGACGGTG CAATCGGGTC CAGCGGGACG CCCATCTGCC GCCAGGCCCA GACTAAGCTG 
GAGGTTGCTC TGTACATGTT TCTGTGGAGT CCCGACATTG AAGCCGTGCT CGTGGCCATG 
TCCTGCTTTC GACACCTGTG TGAGGAAGCC GACATCAGGT GTGGCATAGA TGAGATCTCG 
GTGCACCATT TCTTGCCAAA CTATAACACC TTCATGGAGT TCGCATCTGT CAGCAACATG 
ATGTCGACAG GAAGAGCAGC TCTTCAGAAA AGAGTGATGG CCCTGCTCAG GCGCATCGAA 
CACCCCACCG CTGGAAACAC CGAGGCTTGG GAAGATACTT ACGCCAAATG GGAGCAAGCT 
ACAAAATCAA TCCTCAATTA TCCGAAAGCC AAAATTGAAG ATGGCCAGGT TGCTGAGAGT 
CTTCACAAGA CCATTGTGAA GAGAAGAATG TCTCACGTGA GTGGAGGGGG CTCCATAGAC 
TTGTGCGACA CTGACTCGCT ACAGGAGTGG ATCAACATGA CTGGGTTTCT CTGTGCCCTT 
GGTGGCGTGT GCCTACAGCA GCGGAGCAAT GCCGGCTTTG CCACCTACAG CCCTCCCATG 
GGCCCTATCA GTGAACGCAA GGGCTCTCTC ATTTCCATGG TGTCCAGCGA TGGAAACGCA 
GAGACGCCTG TCAGCAAGTT CATGGATCGG CTGCTCTCCT TGATGGTGTG TAACCACGAA 
AAAGTGGGGC TGCAGATACG AAGCAATGTC AAGGACTTGT TGGGTCTTGA ATTGAGTCCT 
GCCCTTTATC CAATGCTGTT TAACAAATTA AAAAATACCA TCAGCAAGTT TTTTGATCCT 
CAAGGACAGG TTTTATTGAC TGACACCAAC ACCCAGTTTG TGGAGCAGAC CATAGCCATA 
ATGAAGAACT TGCTTGATAA TCATGCTGAG GGTAGTTCTG AACACATGGG ACAAGCTAGC 
ATCGAAACTA TGATGTTAAA TCTGGTCAGA TATGTTAGAG TGTTTGGGAA TTTGGTGCAG 
GCAATTCAGA TCAAAACTAA GCTGTGCCAG CTGGTTCAAG TAATGATGGA GAGGAGAGAT 
GACCTTTCAT TTTGCCAAGA GATGAAATTT AGGAATAAAA TGGTAGAATA TCTGACAGAT 
TGGGTCATGG GAACATCTAA CCAAGCTGCA GATGAAGATG TGAAATGTCT AACTAGAGAT 
TTGGACCAGG CAAGCATGGA AGCAGTGGTG TCATTGCTCG CTGGGCTCCC TCTGCAGCCT 
GAGGAAGGCG ATGGGGTGGA ATTGATGGAA GCCAAATCCC AGCTGTTCCT TAAGTATTTC 
ACCCTCTTTA TGAACCTTCT TAATGACTGC AGTGAGGTCG AAGAAGAAAA TGTCCAGATT 
GGGGGCAGGA AGCGCGGCAT GTCGCGAAGG CTGGCGTCCC TGCGCCACTG CACCGTGCTG 
GCAATGTCCA ACCTGCTCAA TGCGAATGTA GACAGCGGCC TGATGCACTC TATAGGATTG 
GGTTACCACA AAGATCTTCA GACAAGAGCC ACCTTCATGG AAGTCCTCAC AAAAATCCTT 
CAGCAAGGCA CAGAGTTTGA TACGCTCGCA GAAACCGTGT TAGCTGATCG ATTTGAGAGA 
CTTGTTGAGT TGGTCACGAT GATGGGTGAT CAAGGAGAGC TCCCCATAGC CATGGCTCTG 
GCCAACGTGG TGCCTTGTTC TCAGTGGGAT GAACTTGCTC GAGTCCTTGT TACTCTCTTT 
GATTCCCGAC ACTTACTGTA TCAGCTCCTT TGGAACATGT TTTCTAAGGA AGTTGAGCTG 
GCAGACTCCA TGCAGACACT TTTTAGAGGA AACAGCTTGG CCAGTAAAAT CATGACCTTC 
TGCTTTAAGG TCTACGGTGC TACATACCTG CAGAAGCTTC TGGAGCCTTT ACTCCGCTTT 
GTGATCACAT CCTCCGAATG GCAGCACGTC AGCTTCGAGG TCGATCCCAC CAGGTTAGAG 
CATACTGAGA GCCTCGAGGA CAACCAGCGG AACCTTTTGC AAATGACTGA GAAGTTCTTC 
CACGCCATCA TCAGTTCTTC CTCCGAGTTC CCCCCTCAGC TACGCAGTGT GTGCCACTGC 
CTGTACCAGG CAACTTGCCA CTCTCTACTG AATAAAGCTA CCGTAAAAGA AAAAAAGGAA 
AACAAAAAAT CAGTTGTTAG CCAGAGATTC CCTCAGAACA GCATTGGCGC CGTGGGGAGT 
GCCATGTTCC TGAGATTCAT CAACCCTGCC GTTGTCTCTC CCTACGAGGC AGGGATTTTA 
GAGAGAAAAC CGCTCCCTAG AATTGAAAGG GGGTTGAAGC TGATGTCAAA GATTCTTCAG 
AGCATTGCCA ATCATGTCCT TTTCACAAAA GAAGAGTATA TGAGGCCTTT TAACGATTTT 
GTGAAAAGCA ACTTTGATGT AGCACGGAGA TTTTTCCTTG ATATCGCATC TGATTGTCCT 
GTAAGTGATG CCATAAACCA CAGCCTTTCC TTCATCAGCG ATGGCAATGT CTTGGCTTTG 
CACCGTCTGC TCTGGAACAA CCAAGAGAAA ATCGGCCAAT ATCTTTCAAG CAACAGAGAT 
CACAAAGCTG TTGGAAGGCG ACCTTTCGAT AAGATGGCAA CACTGCTTGC CTACCTGGGA 
CCTCCAGAGC ACAAGCCTGT GGCTGACACA CACTGGTCCA GCCTAAATCT TACCAGCTCC 
AAGTTTGAGG AATTCATGAC AAGGCACCAG GTACATGAAA AAGAGGAATT CAAGGCTCTG 
AAAACATTAA ATATCTTCTA TCAAGCAGGC ACTTCCAAAG CTGGAAATCC TGTTTTTTAT 
TATGTCACAC GAAGGTTCAA GACTGGCCAG ATCAACGGGG ATTTGCTTAT ATACCACGTC 
TTACTAACTT TAAAGCCATA TTATGCAAAG CCATATGAGA TTGTAATGGA CCTCACTCAT 
GCCGGGCCTA GCAATCGCTT TAAGACAGAC TTCCTCTCCA AGTGGTTTGT TGTTTTCCCG 
GGCTTTGCTT ACGAGAACGT CTCTTCGGTC TACATCTACA ACTGTAATTC TTGGGTTCGG 
GAGTACACCA AATACCATGA GAGACTGCTC ACGGGACTCA AAGGCAGCAA AAGACTCTTT 
TTCATAGACT CTCCCTCAAA ACTGGCTGAG CATATCGATT ATGATCAGCA AAAACTGCCA 
GCCGCGACCT TGGCTCTGGA TGAAGACCTC AAGGTGTACA ACAGTGCTCT CAAGCTGGCT 
CACAAGGACA CCAAGGTGAC CCTCAAGGTT GGCTCTACTG CGATCCAAGT GACGTCATCT 
GAGCGAACTA AAGTCCTGGG ACAGACGGTT TTTCTGAATG ATATTTACTA TGCCTCTGAG 
ATCGAAGAAA TCTGTCTTGT GGATGAAAAC CAGTTCACTC TGACCATTTC CAATCAGGGC 
ACTCCCCTCA CCTTCATGCA TCAAGAGTGT GAAGGCATTG TGGCGTCCAT CATTCACATC 
CGAACTCGAT GGGAGCTGTC CCAGCCAGAT TCTATCCCCC AGCACACTAA GATCCGGCCA 
AAGGACGTTC CCGGAACACT TTTGAATATC GCATTACTTA ACTTGGGTAG TTCGGACCCA 
AGTTTACGGT CAGCCGCCTA TAATCTTCTC TGTGCCTTAA CCTGTACCTT TAATTTAAAA 
ATTGAGGGCC AATTACTAGA AACATCAGGT CTGTGTATAC CTGCCAACAA TACCCTTTTT 
ATTGTCTCTA TTAGTAAAAC CCTGGCAGCT AATGAGCCAC ACCTCACCTT AGAGTTTTTG 
GAAGAGTGCA TTTCCGGATT TAGCAAATCC AGTATTGAAC TGAAGCACCT GTGTCTAGAA 
TACATGACTC CCTGGCTATC AAATCTAGTC CGTTTCTGTA AACATAATGA TGATGCCAAA 
CGACAGAGAG TCACTGCTAT TCTTGATAAA CTTATAACAA TGACCATAAA TGAAAAGCAG 
ATGTACCCAT CGATCCAAGC CAAGATATGG GGAAGCCTTG GGCAGATTAC AGATCTGCTC 
GATGTAGTGC TGGACAGCTT TATCAAAACC AGTGCCACAG GTGGTTTAGG ATCCATCAAG 
GCTGAAGTGA TGGCTGACAC GGCCGTGGCT CTGGCTTCTG GGAACGTCAA ACTAGTTTCG 
AGCAAGGTGA TTGGAAGAAT GTGCAAAATC ATTGACAAGA CCTGCCTCTC TCCAACTCCT 
ACCTTAGAGC AGCACCTCAT GTGGGATGAT ATCGCCATTT TAGCACGCTA CATGCTAATG 
CTGTCCTTCA ATAATTCTCT GGATGTGGCG GCTCATCTTC CCTACCTCTT CCATGTGGTG 
ACTCTGTTGG TGGCCACAGG TCCCTTGTCC CTTCGTGCTT CCACACACGG ACTGGTCATC 
AATATCATCC ATTCTCTGTG CACGTGCTCA CAGCTTCACT TTAGTGAAGA GACCAAGCAA 
GTTTTACGAC TGAGTCTGAC TGAGTTCTCA CTACCTAAGT TTTATTTGCT GTTCGGCATC 
AGCAAAGTCA AATCAGCTGC CGTGATAGCC TTCCGGTCCA GTTACCGGGA CAGGTCTTTC 
TCCCCTGGCT CCTATGAGAG GGAGACCTTT GCCCTGGCCT CCTTGGAAAC AGTCACAGAA 
GCTCTCTTGG AGATCATGGA GGCTTGTATG AGAGATATTC CTGCATGCAA GTGGCTGGAC 
CAGTGGACAG AGCTAGCCCA GAGGTTTGCG TTCCAGTACA ATCCCTCCCT GCAGCCACGA 
GCTCTGGTTG TGTTTGGTTG TATCAGCAAA AGAGTGTCCC ATGGGCAGAT CAAGCAGATT 
ATCCGCATTC TTAGCAAGGG ACTTGAGAGC TGCCTAAAAG GACCTGATAA TTATAACAGT 
CAAGTTCTGA TTGAAGCTAC CGTGATAGCT CTAACCAAAT TACAACCACT TCTTAATAAG 
GAGTCCCCTC TGCACCGAGC TCTCTTCTGG GTGGCCGTTG CCGTGCTGCA GCTGGATGAA 
GTGGACCTGT ACTCGGCGGG CACTGCCCTC CTCGAACAGA ACCTGCACAC CTTAGACAGC 
CTCCTGCACA TCTTCAACGA CCAGAGCCCA GAAGAAGTGT TCATGGAGAT CCGGCAGCCT 
CTGGAGTGGC ACTGCAAGCA GATGGACCAC TTTGTGGGGC TCAACTTTAA CTCCAGCTTT 
AACTTTGCCC TGGTCGGGCA CCTGCTGAAG GGATTTCGGC ATCCTTCACC TGTCACTGTT 
GCAAGAACTG TGAGGATTTT GCACACCCTA CTAGCATTGG TTAACAAGCA TAGGAATTGT 
GACAAGTTTG AGGTGAACAC CCAGAGTGTG GCTTACTTAG CAGCTTTACT TACTGTGTCT 
GAGGAGGTTC GAAGTCGCTG CAGCTTAAAG CATAGGAAAT CACTCCTACT AACTGATATC 
ACAATGGAAA ATGTGCCTAT GGATATATAT CCCATCTATC ACAGTGACCC CAGCCTTAGG 
ACACTAAAAG AAACACAACC GTGGTCCTCT CCAAAGGGAT CTGAGAGACA TCTTGCAGCC 
AGCTACCCTC CTGTGGGCCA AACTAGCCCC CGAACCAGAA AATCCATGAG CCTGGATATG 
GGGCAGCCTT CCCAGGCCAA CACAAAGAAG TTGCTTGGGA CAAGGAAAAG CTTCGATCAT 
TTGATCTCTG ATGCCAAAGC TCCCAAAAGG CAAGAACAGG AGTCTGGAGT CACCACGCCC 
CCCAAGATGC GGAGAGTGGC CGAGAACGAC TACGAGATGG AAACCCAGAG AATCTCCCCT 
CAGCCACACC CGCCTCTGCG GAAAGTGTCC GTGTCCGAAT CCAATGTGCT GCTGGACGAG 
GAAGTGCTCA CAGACCCCAA AACCCAGGCT CTTCTTCTCA CTGTCCTGGC TACTCTGGTG 
AAGTACACTG CGGACGAGTT TGACCAGCGC ATTCTCTATG AGTACTTAGC GGAGGCCAGT 
GTTGTCTTCC CAAAGGTTTT TCCAGTGGTA CATAATTTGT TGGACTCAAA GATAAATACT 
CTGCTCTCAT TATGCCAAGA CCCAAATTTG CTAAACCCGG TTCATGGAAT TGTGCAGAGT 
GTGGTCTACC ACGAAGAGGC CCCGCCGCAG TACCAGACGG CGTACCTCCA GAGTTTCGGT 
TTTAATGGTC TGTGGAGGTT TGCAGGACCA TTTTCTAAGC AGACGCAGAT CCCAGACTAT 
GCCGAGCTCA TCGTGAAGTT TCTCGATGCC TTGATTGACA CGTATCTGCC TGGAATTGAT 
GAGGAAGCCA GTGAGGAGTC CCTCCTGACA CCCACCTCTC CCTACCCGCC CGCGGTGCAG 
AGCCAGCTTA GCATCACTGC GAACCTCAAC CTCTCTAACT CCATGACCTC ACTGGCAACT 
TCCCAGCATT CCCCAGGCAT CGACAAGGAG AATGTGGAGC TCTCGCCAAC CACCGGGCAC 
TGCAGCGGCG GGAGGACGCG CCACGGCTCC GCCAGCCAGG TCCAGAAGCA AAGGAGTGCC 
GGCAGCTTCA AGAGGCACAG CATCAAGAAG ATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823514.1
CDS	9..8507
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967654.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967654.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGCAGCCA AAGGACACGA TGAGGTTAGA TGAAACAATG 
CTGGTCAAAC AGTTGCTGCC GGAAATCTGC CATTTCCTTC ACACCTACCG GGAAGGGAAC 
CAGCACGCAG CCGAGCTTCG GAGCTCCGCC TCTGGCGTCC TTTTCTCCCT CAGCTGTAAT 
AACTTCAATG CCGTCTTCAG CCGGATTTCC ACCAGGTTAC AGGAATTGAC TGTTTGCTCA 
GAAGATAATG TTGATGTCCA TGATATAGAA TTATTACAAT ATATCAATGT GGATTGTGCA 
AAACTAAAGC GGCTCTTGCA GGAAACTGTA TTTAAGTTTA AAGCCTTAAA GAAGGTAGCT 
CAACTAGCAG TTATAAATAG CCTAGAAAAG GCATTTTGGA ATTGGGTAGA GAACTATCCT 
GATGAATTTA CAAAGCTGTA CCAGACTCCC CAGACAGATA TGGCGGAATG TGCAGAAAAA 
TTATTTGACT TGGTGGATGG TTTTGCTGAA AGCACCAAAC GTAAAGCTGC AGTTTGGCCA 
CTGCAAATCA TTCTTCTTGT CTTGTGTCCA GAAATAATCC AGGACATTTC CAAGGATGTG 
GTTGATGAAG CGAAAATGAA TAAGAAAATG TTTCTCGACA ATCTCAGAAG AGCGCTGGCC 
GGCCACAGTG GGAGCAGGCA GCTGACAGAG AGTGCCGCGA TCGCTTGTGT TAAACTGTGC 
AAAGCCAGCA CCTACATCAA CTGGGAGGAT AACTCGGTCA TTTTCCTGCT CGTCCAATCC 
ATGGTGGTGG ATCTGAAGAA CTTGCTCTTT AATCCCAGTA AGCCCTTCTC CAGAGGCAGC 
CAGAATGCTG ATGTGGACCT CATGATAGAC TGCCTGGTTT CTTGCTTTCG CATCAGCCCT 
CACAACAACC AACACTTTAA GATCTGTTTG GCCCAGAACT CCCCATCTAC ATTTCATTAT 
GTGCTGGTAA ACTCCCTCCA TCGAATTATC ACCAATTCCG CCCTGGATTG GTGGCCCAAG 
ATTGATGCTG TGTATTGCCA TTCTGTAGAA CTTCGAAACA TGTTTGGTGA AACGCTTCAC 
AAGGCCGTGC AGGGCTGTGG GCCTCACCCC GCGATACGAA TGGCGCCGAG TCTTACATTT 
AAAGAAAAAA TGACAAGCCT TAAATTTAAA GAAAAACCTA CGGACTTGGA AACAAGAAGT 
TATAAATACC TCTTATTATC CATGGTGAAA CTAATCCATG CAGATCCAAA GCTGTTGCTA 
TGTAATCCAA GAAAACAAGG GCCTGAAACT CAGGGGAGTA CAGCAGAGTT AATCACAGGC 
CTTGTGCAGC TCGTCCCACA GTCCAGCATG CCAGAAATAG CTCAAGAAGC AATGGAGGCT 
TTGCTGGTTC TTCATCAGTT GGATAGTATT GACTTATGGA ATCCAGATTC ACCTATAGAA 
ACATTCTGGG AGATTAGCTC ACAAATGCTT TTTTATATCT GTAAAAAGCT AACTACCCAT 
CAGATGCTTA GCAGTACAGA AATTCTCAAG TGGTTGCGAG AAATTTTGAT CTGTAGGAAC 
AAATATCTTC TTAAAAATAA GCAGTCAGAT AGGAGTTCTT CCCATTTCCT CTACCTTTAT 
GGGGCAGGCT ATGATGTCTC TTCTGGTGGA GGTACCAGTC ATATCTCTCT AGACCACGAG 
GAGTTTATGC GTACCTGTGC TCATGGACCA TCTCTCAGGA AAGTGAAAGG AAGCTCCTCA 
ATGGACGGTG CAATCGGGTC CAGCGGGACG CCCATCTGCC GCCAGGCCCA GACTAAGCTG 
GAGGTTGCTC TGTACATGTT TCTGTGGAGT CCCGACATTG AAGCCGTGCT CGTGGCCATG 
TCCTGCTTTC GACACCTGTG TGAGGAAGCC GACATCAGGT GTGGCATAGA TGAGATCTCG 
GTGCACCATT TCTTGCCAAA CTATAACACC TTCATGGAGT TCGCATCTGT CAGCAACATG 
ATGTCGACAG GAAGAGCAGC TCTTCAGAAA AGAGTGATGG CCCTGCTCAG GCGCATCGAA 
CACCCCACCG CTGGAAACAC CGAGGCTTGG GAAGATACTT ACGCCAAATG GGAGCAAGCT 
ACAAAATCAA TCCTCAATTA TCCGAAAGCC AAAATTGAAG ATGGCCAGGT TGCTGAGAGT 
CTTCACAAGA CCATTGTGAA GAGAAGAATG TCTCACGTGA GTGGAGGGGG CTCCATAGAC 
TTGTGCGACA CTGACTCGCT ACAGGAGTGG ATCAACATGA CTGGGTTTCT CTGTGCCCTT 
GGTGGCGTGT GCCTACAGCA GCGGAGCAAT GCCGGCTTTG CCACCTACAG CCCTCCCATG 
GGCCCTATCA GTGAACGCAA GGGCTCTCTC ATTTCCATGG TGTCCAGCGA TGGAAACGCA 
GAGACGCCTG TCAGCAAGTT CATGGATCGG CTGCTCTCCT TGATGGTGTG TAACCACGAA 
AAAGTGGGGC TGCAGATACG AAGCAATGTC AAGGACTTGT TGGGTCTTGA ATTGAGTCCT 
GCCCTTTATC CAATGCTGTT TAACAAATTA AAAAATACCA TCAGCAAGTT TTTTGATCCT 
CAAGGACAGG TTTTATTGAC TGACACCAAC ACCCAGTTTG TGGAGCAGAC CATAGCCATA 
ATGAAGAACT TGCTTGATAA TCATGCTGAG GGTAGTTCTG AACACATGGG ACAAGCTAGC 
ATCGAAACTA TGATGTTAAA TCTGGTCAGA TATGTTAGAG TGTTTGGGAA TTTGGTGCAG 
GCAATTCAGA TCAAAACTAA GCTGTGCCAG CTGGTTCAAG TAATGATGGA GAGGAGAGAT 
GACCTTTCAT TTTGCCAAGA GATGAAATTT AGGAATAAAA TGGTAGAATA TCTGACAGAT 
TGGGTCATGG GAACATCTAA CCAAGCTGCA GATGAAGATG TGAAATGTCT AACTAGAGAT 
TTGGACCAGG CAAGCATGGA AGCAGTGGTG TCATTGCTCG CTGGGCTCCC TCTGCAGCCT 
GAGGAAGGCG ATGGGGTGGA ATTGATGGAA GCCAAATCCC AGCTGTTCCT TAAGTATTTC 
ACCCTCTTTA TGAACCTTCT TAATGACTGC AGTGAGGTCG AAGAAGAAAA TGTCCAGATT 
GGGGGCAGGA AGCGCGGCAT GTCGCGAAGG CTGGCGTCCC TGCGCCACTG CACCGTGCTG 
GCAATGTCCA ACCTGCTCAA TGCGAATGTA GACAGCGGCC TGATGCACTC TATAGGATTG 
GGTTACCACA AAGATCTTCA GACAAGAGCC ACCTTCATGG AAGTCCTCAC AAAAATCCTT 
CAGCAAGGCA CAGAGTTTGA TACGCTCGCA GAAACCGTGT TAGCTGATCG ATTTGAGAGA 
CTTGTTGAGT TGGTCACGAT GATGGGTGAT CAAGGAGAGC TCCCCATAGC CATGGCTCTG 
GCCAACGTGG TGCCTTGTTC TCAGTGGGAT GAACTTGCTC GAGTCCTTGT TACTCTCTTT 
GATTCCCGAC ACTTACTGTA TCAGCTCCTT TGGAACATGT TTTCTAAGGA AGTTGAGCTG 
GCAGACTCCA TGCAGACACT TTTTAGAGGA AACAGCTTGG CCAGTAAAAT CATGACCTTC 
TGCTTTAAGG TCTACGGTGC TACATACCTG CAGAAGCTTC TGGAGCCTTT ACTCCGCTTT 
GTGATCACAT CCTCCGAATG GCAGCACGTC AGCTTCGAGG TCGATCCCAC CAGGTTAGAG 
CATACTGAGA GCCTCGAGGA CAACCAGCGG AACCTTTTGC AAATGACTGA GAAGTTCTTC 
CACGCCATCA TCAGTTCTTC CTCCGAGTTC CCCCCTCAGC TACGCAGTGT GTGCCACTGC 
CTGTACCAGG CAACTTGCCA CTCTCTACTG AATAAAGCTA CCGTAAAAGA AAAAAAGGAA 
AACAAAAAAT CAGTTGTTAG CCAGAGATTC CCTCAGAACA GCATTGGCGC CGTGGGGAGT 
GCCATGTTCC TGAGATTCAT CAACCCTGCC GTTGTCTCTC CCTACGAGGC AGGGATTTTA 
GAGAGAAAAC CGCTCCCTAG AATTGAAAGG GGGTTGAAGC TGATGTCAAA GATTCTTCAG 
AGCATTGCCA ATCATGTCCT TTTCACAAAA GAAGAGTATA TGAGGCCTTT TAACGATTTT 
GTGAAAAGCA ACTTTGATGT AGCACGGAGA TTTTTCCTTG ATATCGCATC TGATTGTCCT 
GTAAGTGATG CCATAAACCA CAGCCTTTCC TTCATCAGCG ATGGCAATGT CTTGGCTTTG 
CACCGTCTGC TCTGGAACAA CCAAGAGAAA ATCGGCCAAT ATCTTTCAAG CAACAGAGAT 
CACAAAGCTG TTGGAAGGCG ACCTTTCGAT AAGATGGCAA CACTGCTTGC CTACCTGGGA 
CCTCCAGAGC ACAAGCCTGT GGCTGACACA CACTGGTCCA GCCTAAATCT TACCAGCTCC 
AAGTTTGAGG AATTCATGAC AAGGCACCAG GTACATGAAA AAGAGGAATT CAAGGCTCTG 
AAAACATTAA ATATCTTCTA TCAAGCAGGC ACTTCCAAAG CTGGAAATCC TGTTTTTTAT 
TATGTCACAC GAAGGTTCAA GACTGGCCAG ATCAACGGGG ATTTGCTTAT ATACCACGTC 
TTACTAACTT TAAAGCCATA TTATGCAAAG CCATATGAGA TTGTAATGGA CCTCACTCAT 
GCCGGGCCTA GCAATCGCTT TAAGACAGAC TTCCTCTCCA AGTGGTTTGT TGTTTTCCCG 
GGCTTTGCTT ACGAGAACGT CTCTTCGGTC TACATCTACA ACTGTAATTC TTGGGTTCGG 
GAGTACACCA AATACCATGA GAGACTGCTC ACGGGACTCA AAGGCAGCAA AAGACTCTTT 
TTCATAGACT CTCCCTCAAA ACTGGCTGAG CATATCGATT ATGATCAGCA AAAACTGCCA 
GCCGCGACCT TGGCTCTGGA TGAAGACCTC AAGGTGTACA ACAGTGCTCT CAAGCTGGCT 
CACAAGGACA CCAAGGTGAC CCTCAAGGTT GGCTCTACTG CGATCCAAGT GACGTCATCT 
GAGCGAACTA AAGTCCTGGG ACAGACGGTT TTTCTGAATG ATATTTACTA TGCCTCTGAG 
ATCGAAGAAA TCTGTCTTGT GGATGAAAAC CAGTTCACTC TGACCATTTC CAATCAGGGC 
ACTCCCCTCA CCTTCATGCA TCAAGAGTGT GAAGGCATTG TGGCGTCCAT CATTCACATC 
CGAACTCGAT GGGAGCTGTC CCAGCCAGAT TCTATCCCCC AGCACACTAA GATCCGGCCA 
AAGGACGTTC CCGGAACACT TTTGAATATC GCATTACTTA ACTTGGGTAG TTCGGACCCA 
AGTTTACGGT CAGCCGCCTA TAATCTTCTC TGTGCCTTAA CCTGTACCTT TAATTTAAAA 
ATTGAGGGCC AATTACTAGA AACATCAGGT CTGTGTATAC CTGCCAACAA TACCCTTTTT 
ATTGTCTCTA TTAGTAAAAC CCTGGCAGCT AATGAGCCAC ACCTCACCTT AGAGTTTTTG 
GAAGAGTGCA TTTCCGGATT TAGCAAATCC AGTATTGAAC TGAAGCACCT GTGTCTAGAA 
TACATGACTC CCTGGCTATC AAATCTAGTC CGTTTCTGTA AACATAATGA TGATGCCAAA 
CGACAGAGAG TCACTGCTAT TCTTGATAAA CTTATAACAA TGACCATAAA TGAAAAGCAG 
ATGTACCCAT CGATCCAAGC CAAGATATGG GGAAGCCTTG GGCAGATTAC AGATCTGCTC 
GATGTAGTGC TGGACAGCTT TATCAAAACC AGTGCCACAG GTGGTTTAGG ATCCATCAAG 
GCTGAAGTGA TGGCTGACAC GGCCGTGGCT CTGGCTTCTG GGAACGTCAA ACTAGTTTCG 
AGCAAGGTGA TTGGAAGAAT GTGCAAAATC ATTGACAAGA CCTGCCTCTC TCCAACTCCT 
ACCTTAGAGC AGCACCTCAT GTGGGATGAT ATCGCCATTT TAGCACGCTA CATGCTAATG 
CTGTCCTTCA ATAATTCTCT GGATGTGGCG GCTCATCTTC CCTACCTCTT CCATGTGGTG 
ACTCTGTTGG TGGCCACAGG TCCCTTGTCC CTTCGTGCTT CCACACACGG ACTGGTCATC 
AATATCATCC ATTCTCTGTG CACGTGCTCA CAGCTTCACT TTAGTGAAGA GACCAAGCAA 
GTTTTACGAC TGAGTCTGAC TGAGTTCTCA CTACCTAAGT TTTATTTGCT GTTCGGCATC 
AGCAAAGTCA AATCAGCTGC CGTGATAGCC TTCCGGTCCA GTTACCGGGA CAGGTCTTTC 
TCCCCTGGCT CCTATGAGAG GGAGACCTTT GCCCTGGCCT CCTTGGAAAC AGTCACAGAA 
GCTCTCTTGG AGATCATGGA GGCTTGTATG AGAGATATTC CTGCATGCAA GTGGCTGGAC 
CAGTGGACAG AGCTAGCCCA GAGGTTTGCG TTCCAGTACA ATCCCTCCCT GCAGCCACGA 
GCTCTGGTTG TGTTTGGTTG TATCAGCAAA AGAGTGTCCC ATGGGCAGAT CAAGCAGATT 
ATCCGCATTC TTAGCAAGGG ACTTGAGAGC TGCCTAAAAG GACCTGATAA TTATAACAGT 
CAAGTTCTGA TTGAAGCTAC CGTGATAGCT CTAACCAAAT TACAACCACT TCTTAATAAG 
GAGTCCCCTC TGCACCGAGC TCTCTTCTGG GTGGCCGTTG CCGTGCTGCA GCTGGATGAA 
GTGGACCTGT ACTCGGCGGG CACTGCCCTC CTCGAACAGA ACCTGCACAC CTTAGACAGC 
CTCCTGCACA TCTTCAACGA CCAGAGCCCA GAAGAAGTGT TCATGGAGAT CCGGCAGCCT 
CTGGAGTGGC ACTGCAAGCA GATGGACCAC TTTGTGGGGC TCAACTTTAA CTCCAGCTTT 
AACTTTGCCC TGGTCGGGCA CCTGCTGAAG GGATTTCGGC ATCCTTCACC TGTCACTGTT 
GCAAGAACTG TGAGGATTTT GCACACCCTA CTAGCATTGG TTAACAAGCA TAGGAATTGT 
GACAAGTTTG AGGTGAACAC CCAGAGTGTG GCTTACTTAG CAGCTTTACT TACTGTGTCT 
GAGGAGGTTC GAAGTCGCTG CAGCTTAAAG CATAGGAAAT CACTCCTACT AACTGATATC 
ACAATGGAAA ATGTGCCTAT GGATATATAT CCCATCTATC ACAGTGACCC CAGCCTTAGG 
ACACTAAAAG AAACACAACC GTGGTCCTCT CCAAAGGGAT CTGAGAGACA TCTTGCAGCC 
AGCTACCCTC CTGTGGGCCA AACTAGCCCC CGAACCAGAA AATCCATGAG CCTGGATATG 
GGGCAGCCTT CCCAGGCCAA CACAAAGAAG TTGCTTGGGA CAAGGAAAAG CTTCGATCAT 
TTGATCTCTG ATGCCAAAGC TCCCAAAAGG CAAGAACAGG AGTCTGGAGT CACCACGCCC 
CCCAAGATGC GGAGAGTGGC CGAGAACGAC TACGAGATGG AAACCCAGAG AATCTCCCCT 
CAGCCACACC CGCCTCTGCG GAAAGTGTCC GTGTCCGAAT CCAATGTGCT GCTGGACGAG 
GAAGTGCTCA CAGACCCCAA AACCCAGGCT CTTCTTCTCA CTGTCCTGGC TACTCTGGTG 
AAGTACACTG CGGACGAGTT TGACCAGCGC ATTCTCTATG AGTACTTAGC GGAGGCCAGT 
GTTGTCTTCC CAAAGGTTTT TCCAGTGGTA CATAATTTGT TGGACTCAAA GATAAATACT 
CTGCTCTCAT TATGCCAAGA CCCAAATTTG CTAAACCCGG TTCATGGAAT TGTGCAGAGT 
GTGGTCTACC ACGAAGAGGC CCCGCCGCAG TACCAGACGG CGTACCTCCA GAGTTTCGGT 
TTTAATGGTC TGTGGAGGTT TGCAGGACCA TTTTCTAAGC AGACGCAGAT CCCAGACTAT 
GCCGAGCTCA TCGTGAAGTT TCTCGATGCC TTGATTGACA CGTATCTGCC TGGAATTGAT 
GAGGAAGCCA GTGAGGAGTC CCTCCTGACA CCCACCTCTC CCTACCCGCC CGCGGTGCAG 
AGCCAGCTTA GCATCACTGC GAACCTCAAC CTCTCTAACT CCATGACCTC ACTGGCAACT 
TCCCAGCATT CCCCAGGCAT CGACAAGGAG AATGTGGAGC TCTCGCCAAC CACCGGGCAC 
TGCAGCGGCG GGAGGACGCG CCACGGCTCC GCCAGCCAGG TCCAGAAGCA AAGGAGTGCC 
GGCAGCTTCA AGAGGCACAG CATCAAGAAG ATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967655.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823515.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X16, mRNA.

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CloneID	OSa234703
Clone ID Related Accession (Same CDS sequence)	XM_031967655.1
Accession Version	XM_031967655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGGACGGT 
GCAATCGGGT CCAGCGGGAC GCCCATCTGC CGCCAGGCCC AGACTAAGCT GGAGGTTGCT 
CTGTACATGT TTCTGTGGAG TCCCGACATT GAAGCCGTGC TCGTGGCCAT GTCCTGCTTT 
CGACACCTGT GTGAGGAAGC CGACATCAGG TGTGGCATAG ATGAGATCTC GGTGCACCAT 
TTCTTGCCAA ACTATAACAC CTTCATGGAG TTCGCATCTG TCAGCAACAT GATGTCGACA 
GGAAGAGCAG CTCTTCAGAA AAGAGTGATG GCCCTGCTCA GGCGCATCGA ACACCCCACC 
GCTGGAAACA CCGAGGCTTG GGAAGATACT TACGCCAAAT GGGAGCAAGC TACAAAATCA 
ATCCTCAATT ATCCGAAAGC CAAAATTGAA GATGGCCAGG TTGCTGAGAG TCTTCACAAG 
ACCATTGTGA AGAGAAGAAT GTCTCACGTG AGTGGAGGGG GCTCCATAGA CTTGTGCGAC 
ACTGACTCGC TACAGGAGTG GATCAACATG ACTGGGTTTC TCTGTGCCCT TGGTGGCGTG 
TGCCTACAGC AGCGGAGCAA TGCCGGCTTT GCCACCTACA GCCCTCCCAT GGGCCCTATC 
AGTGAACGCA AGGGCTCTCT CATTTCCATG GTGTCCAGCG ATGGAAACGC AGAGACGCCT 
GTCAGCAAGT TCATGGATCG GCTGCTCTCC TTGATGGTGT GTAACCACGA AAAAGTGGGG 
CTGCAGATAC GAAGCAATGT CAAGGACTTG TTGGGTCTTG AATTGAGTCC TGCCCTTTAT 
CCAATGCTGT TTAACAAATT AAAAAATACC ATCAGCAAGT TTTTTGATCC TCAAGGACAG 
GTTTTATTGA CTGACACCAA CACCCAGTTT GTGGAGCAGA CCATAGCCAT AATGAAGAAC 
TTGCTTGATA ATCATGCTGA GGGTAGTTCT GAACACATGG GACAAGCTAG CATCGAAACT 
ATGATGTTAA ATCTGGTCAG ATATGTTAGA GTGTTTGGGA ATTTGGTGCA GGCAATTCAG 
ATCAAAACTA AGCTGTGCCA GCTGGTTCAA GTAATGATGG AGAGGAGAGA TGACCTTTCA 
TTTTGCCAAG AGATGAAATT TAGGAATAAA ATGGTAGAAT ATCTGACAGA TTGGGTCATG 
GGAACATCTA ACCAAGCTGC AGATGAAGAT GTGAAATGTC TAACTAGAGA TTTGGACCAG 
GCAAGCATGG AAGCAGTGGT GTCATTGCTC GCTGGGCTCC CTCTGCAGCC TGAGGAAGGC 
GATGGGGTGG AATTGATGGA AGCCAAATCC CAGCTGTTCC TTAAGTATTT CACCCTCTTT 
ATGAACCTTC TTAATGACTG CAGTGAGGTC GAAGAAGAAA ATGTCCAGAT TGGGGGCAGG 
AAGCGCGGCA TGTCGCGAAG GCTGGCGTCC CTGCGCCACT GCACCGTGCT GGCAATGTCC 
AACCTGCTCA ATGCGAATGT AGACAGCGGC CTGATGCACT CTATAGTGCT AGGATTGGGT 
TACCACAAAG ATCTTCAGAC AAGAGCCACC TTCATGGAAG TCCTCACAAA AATCCTTCAG 
CAAGGCACAG AGTTTGATAC GCTCGCAGAA ACCGTGTTAG CTGATCGATT TGAGAGACTT 
GTTGAGTTGG TCACGATGAT GGGTGATCAA GGAGAGCTCC CCATAGCCAT GGCTCTGGCC 
AACGTGGTGC CTTGTTCTCA GTGGGATGAA CTTGCTCGAG TCCTTGTTAC TCTCTTTGAT 
TCCCGACACT TACTGTATCA GCTCCTTTGG AACATGTTTT CTAAGGAAGT TGAGCTGGCA 
GACTCCATGC AGACACTTTT TAGAGGAAAC AGCTTGGCCA GTAAAATCAT GACCTTCTGC 
TTTAAGGTCT ACGGTGCTAC ATACCTGCAG AAGCTTCTGG AGCCTTTACT CCGCTTTGTG 
ATCACATCCT CCGAATGGCA GCACGTCAGC TTCGAGGTCG ATCCCACCAG GTTAGAGCAT 
ACTGAGAGCC TCGAGGACAA CCAGCGGAAC CTTTTGCAAA TGACTGAGAA GTTCTTCCAC 
GCCATCATCA GTTCTTCCTC CGAGTTCCCC CCTCAGCTAC GCAGTGTGTG CCACTGCCTG 
TACCAGGCAA CTTGCCACTC TCTACTGAAT AAAGCTACCG TAAAAGAAAA AAAGGAAAAC 
AAAAAATCAG TTGTTAGCCA GAGATTCCCT CAGAACAGCA TTGGCGCCGT GGGGAGTGCC 
ATGTTCCTGA GATTCATCAA CCCTGCCGTT GTCTCTCCCT ACGAGGCAGG GATTTTAGAG 
AGAAAACCGC TCCCTAGAAT TGAAAGGGGG TTGAAGCTGA TGTCAAAGAT TCTTCAGAGC 
ATTGCCAATC ATGTCCTTTT CACAAAAGAA GAGTATATGA GGCCTTTTAA CGATTTTGTG 
AAAAGCAACT TTGATGTAGC ACGGAGATTT TTCCTTGATA TCGCATCTGA TTGTCCTGTA 
AGTGATGCCA TAAACCACAG CCTTTCCTTC ATCAGCGATG GCAATGTCTT GGCTTTGCAC 
CGTCTGCTCT GGAACAACCA AGAGAAAATC GGCCAATATC TTTCAAGCAA CAGAGATCAC 
AAAGCTGTTG GAAGGCGACC TTTCGATAAG ATGGCAACAC TGCTTGCCTA CCTGGGACCT 
CCAGAGCACA AGCCTGTGGC TGACACACAC TGGTCCAGCC TAAATCTTAC CAGCTCCAAG 
TTTGAGGAAT TCATGACAAG GCACCAGGTA CATGAAAAAG AGGAATTCAA GGCTCTGAAA 
ACATTAAATA TCTTCTATCA AGCAGGCACT TCCAAAGCTG GAAATCCTGT TTTTTATTAT 
GTCACACGAA GGTTCAAGAC TGGCCAGATC AACGGGGATT TGCTTATATA CCACGTCTTA 
CTAACTTTAA AGCCATATTA TGCAAAGCCA TATGAGATTG TAATGGACCT CACTCATGCC 
GGGCCTAGCA ATCGCTTTAA GACAGACTTC CTCTCCAAGT GGTTTGTTGT TTTCCCGGGC 
TTTGCTTACG AGAACGTCTC TTCGGTCTAC ATCTACAACT GTAATTCTTG GGTTCGGGAG 
TACACCAAAT ACCATGAGAG ACTGCTCACG GGACTCAAAG GCAGCAAAAG ACTCTTTTTC 
ATAGACTCTC CCTCAAAACT GGCTGAGCAT ATCGATTATG ATCAGCAAAA ACTGCCAGCC 
GCGACCTTGG CTCTGGATGA AGACCTCAAG GTGTACAACA GTGCTCTCAA GCTGGCTCAC 
AAGGACACCA AGGTGACCCT CAAGGTTGGC TCTACTGCGA TCCAAGTGAC GTCATCTGAG 
CGAACTAAAG TCCTGGGACA GACGGTTTTT CTGAATGATA TTTACTATGC CTCTGAGATC 
GAAGAAATCT GTCTTGTGGA TGAAAACCAG TTCACTCTGA CCATTTCCAA TCAGGGCACT 
CCCCTCACCT TCATGCATCA AGAGTGTGAA GGCATTGTGG CGTCCATCAT TCACATCCGA 
ACTCGATGGG AGCTGTCCCA GCCAGATTCT ATCCCCCAGC ACACTAAGAT CCGGCCAAAG 
GACGTTCCCG GAACACTTTT GAATATCGCA TTACTTAACT TGGGTAGTTC GGACCCAAGT 
TTACGGTCAG CCGCCTATAA TCTTCTCTGT GCCTTAACCT GTACCTTTAA TTTAAAAATT 
GAGGGCCAAT TACTAGAAAC ATCAGGTCTG TGTATACCTG CCAACAATAC CCTTTTTATT 
GTCTCTATTA GTAAAACCCT GGCAGCTAAT GAGCCACACC TCACCTTAGA GTTTTTGGAA 
GAGTGCATTT CCGGATTTAG CAAATCCAGT ATTGAACTGA AGCACCTGTG TCTAGAATAC 
ATGACTCCCT GGCTATCAAA TCTAGTCCGT TTCTGTAAAC ATAATGATGA TGCCAAACGA 
CAGAGAGTCA CTGCTATTCT TGATAAACTT ATAACAATGA CCATAAATGA AAAGCAGATG 
TACCCATCGA TCCAAGCCAA GATATGGGGA AGCCTTGGGC AGATTACAGA TCTGCTCGAT 
GTAGTGCTGG ACAGCTTTAT CAAAACCAGT GCCACAGGTG GTTTAGGATC CATCAAGGCT 
GAAGTGATGG CTGACACGGC CGTGGCTCTG GCTTCTGGGA ACGTCAAACT AGTTTCGAGC 
AAGGTGATTG GAAGAATGTG CAAAATCATT GACAAGACCT GCCTCTCTCC AACTCCTACC 
TTAGAGCAGC ACCTCATGTG GGATGATATC GCCATTTTAG CACGCTACAT GCTAATGCTG 
TCCTTCAATA ATTCTCTGGA TGTGGCGGCT CATCTTCCCT ACCTCTTCCA TGTGGTGACT 
CTGTTGGTGG CCACAGGTCC CTTGTCCCTT CGTGCTTCCA CACACGGACT GGTCATCAAT 
ATCATCCATT CTCTGTGCAC GTGCTCACAG CTTCACTTTA GTGAAGAGAC CAAGCAAGTT 
TTACGACTGA GTCTGACTGA GTTCTCACTA CCTAAGTTTT ATTTGCTGTT CGGCATCAGC 
AAAGTCAAAT CAGCTGCCGT GATAGCCTTC CGGTCCAGTT ACCGGGACAG GTCTTTCTCC 
CCTGGCTCCT ATGAGAGGGA GACCTTTGCC CTGGCCTCCT TGGAAACAGT CACAGAAGCT 
CTCTTGGAGA TCATGGAGGC TTGTATGAGA GATATTCCTG CATGCAAGTG GCTGGACCAG 
TGGACAGAGC TAGCCCAGAG GTTTGCGTTC CAGTACAATC CCTCCCTGCA GCCACGAGCT 
CTGGTTGTGT TTGGTTGTAT CAGCAAAAGA GTGTCCCATG GGCAGATCAA GCAGATTATC 
CGCATTCTTA GCAAGGGACT TGAGAGCTGC CTAAAAGGAC CTGATAATTA TAACAGTCAA 
GTTCTGATTG AAGCTACCGT GATAGCTCTA ACCAAATTAC AACCACTTCT TAATAAGCAG 
GAGTCCCCTC TGCACCGAGC TCTCTTCTGG GTGGCCGTTG CCGTGCTGCA GCTGGATGAA 
GTGGACCTGT ACTCGGCGGG CACTGCCCTC CTCGAACAGA ACCTGCACAC CTTAGACAGC 
CTCCTGCACA TCTTCAACGA CCAGAGCCCA GAAGAAGTGT TCATGGAGAT CCGGCAGCCT 
CTGGAGTGGC ACTGCAAGCA GATGGACCAC TTTGTGGGGC TCAACTTTAA CTCCAGCTTT 
AACTTTGCCC TGGTCGGGCA CCTGCTGAAG GGATTTCGGC ATCCTTCACC TGTCACTGTT 
GCAAGAACTG TGAGGATTTT GCACACCCTA CTAGCATTGG TTAACAAGCA TAGGAATTGT 
GACAAGTTTG AGGTGAACAC CCAGAGTGTG GCTTACTTAG CAGCTTTACT TACTGTGTCT 
GAGGAGGTTC GAAGTCGCTG CAGCTTAAAG CATAGGAAAT CACTCCTACT AACTGATATC 
ACAATGGAAA ATGTGCCTAT GGATATATAT CCCATCTATC ACAGTGACCC CAGCCTTAGG 
ACACTAAAAG AAACACAACC GTGGTCCTCT CCAAAGGGAT CTGAGAGACA TCTTGCAGCC 
AGCTACCCTC CTGTGGGCCA AACTAGCCCC CGAACCAGAA AATCCATGAG CCTGGATATG 
GGGCAGCCTT CCCAGGCCAA CACAAAGAAG TTGCTTGGGA CAAGGAAAAG CTTCGATCAT 
TTGATCTCTG ATGCCAAAGC TCCCAAAAGG CAAGAACAGG AGTCTGGAGT CACCACGCCC 
CCCAAGATGC GGAGAGTGGC CGAGAACGAC TACGAGATGG AAACCCAGAG AATCTCCCCT 
CAGCCACACC CGCCTCTGCG GAAAGTGTCC GTGTCCGAAT CCAATGTGCT GCTGGACGAG 
GAAGTGCTCA CAGACCCCAA AACCCAGGCT CTTCTTCTCA CTGTCCTGGC TACTCTGGTG 
AAGTACACTG CGGACGAGTT TGACCAGCGC ATTCTCTATG AGTACTTAGC GGAGGCCAGT 
GTTGTCTTCC CAAAGGTTTT TCCAGTGGTA CATAATTTGT TGGACTCAAA GATAAATACT 
CTGCTCTCAT TATGCCAAGA CCCAAATTTG CTAAACCCGG TTCATGGAAT TGTGCAGAGT 
GTGGTCTACC ACGAAGAGGC CCCGCCGCAG TACCAGACGG CGTACCTCCA GAGTTTCGGT 
TTTAATGGTC TGTGGAGGTT TGCAGGACCA TTTTCTAAGC AGACGCAGAT CCCAGACTAT 
GCCGAGCTCA TCGTGAAGTT TCTCGATGCC TTGATTGACA CGTATCTGCC TGGAATTGAT 
GAGGAAGCCA GTGAGGAGTC CCTCCTGACA CCCACCTCTC CCTACCCGCC CGCGGTGCAG 
AGCCAGCTTA GCATCACTGC GAACCTCAAC CTCTCTAACT CCATGACCTC ACTGGCAACT 
TCCCAGCATT CCCCAGGCAT CGACAAGGAG AATGTGGAGC TCTCGCCAAC CACCGGGCAC 
TGCAGCGGCG GGAGGACGCG CCACGGCTCC GCCAGCCAGG TCCAGAAGCA AAGGAGTGCC 
GGCAGCTTCA AGAGGCACAG CATCAAGAAG ATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823515.1
CDS	9..8507
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGVSDLQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKFSTLLTTITAWILSPWLCDTDVSIPLSCQITRPQSSFKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIVLGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKQESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967655.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967655.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGGTAAGT GATTTGCAGC CAAAGGACAC GATGAGGTTA 
GATGAAACAA TGCTGGTCAA ACAGTTGCTG CCGGAAATCT GCCATTTCCT TCACACCTAC 
CGGGAAGGGA ACCAGCACGC AGCCGAGCTT CGGAGCTCCG CCTCTGGCGT CCTTTTCTCC 
CTCAGCTGTA ATAACTTCAA TGCCGTCTTC AGCCGGATTT CCACCAGGTT ACAGGAATTG 
ACTGTTTGCT CAGAAGATAA TGTTGATGTC CATGATATAG AATTATTACA ATATATCAAT 
GTGGATTGTG CAAAACTAAA GCGGCTCTTG CAGGAAACTG TATTTAAGTT TAAAGCCTTA 
AAGAAGGTAG CTCAACTAGC AGTTATAAAT AGCCTAGAAA AGGCATTTTG GAATTGGGTA 
GAGAACTATC CTGATGAATT TACAAAGCTG TACCAGACTC CCCAGACAGA TATGGCGGAA 
TGTGCAGAAA AATTATTTGA CTTGGTGGAT GGTTTTGCTG AAAGCACCAA ACGTAAAGCT 
GCAGTTTGGC CACTGCAAAT CATTCTTCTT GTCTTGTGTC CAGAAATAAT CCAGGACATT 
TCCAAGGATG TGGTTGATGA AGCGAAAATG AATAAGTTTT CTACACTGTT GACCACTATC 
ACCGCCTGGA TACTTTCTCC TTGGCTTTGT GATACCGATG TCTCAATTCC TCTTTCCTGC 
CAAATCACTC GTCCTCAGTC CTCCTTTAAA ATGTTTCTCG ACAATCTCAG AAGAGCGCTG 
GCCGGCCACA GTGGGAGCAG GCAGCTGACA GAGAGTGCCG CGATCGCTTG TGTTAAACTG 
TGCAAAGCCA GCACCTACAT CAACTGGGAG GATAACTCGG TCATTTTCCT GCTCGTCCAA 
TCCATGGTGG TGGATCTGAA GAACTTGCTC TTTAATCCCA GTAAGCCCTT CTCCAGAGGC 
AGCCAGAATG CTGATGTGGA CCTCATGATA GACTGCCTGG TTTCTTGCTT TCGCATCAGC 
CCTCACAACA ACCAACACTT TAAGATCTGT TTGGCCCAGA ACTCCCCATC TACATTTCAT 
TATGTGCTGG TAAACTCCCT CCATCGAATT ATCACCAATA GCACTTTCAA GCATGGACTT 
GGCACTGCTT CCGCCCTGGA TTGGTGGCCC AAGATTGATG CTGTGTATTG CCATTCTGTA 
GAACTTCGAA ACATGTTTGG TGAAACGCTT CACAAGGCCG TGCAGGGCTG TGGGCCTCAC 
CCCGCGATAC GAATGGCGCC GAGTCTTACA TTTAAAGAAA AAATGACAAG CCTTAAATTT 
AAAGAAAAAC CTACGGACTT GGAAACAAGA AGTTATAAAT ACCTCTTATT ATCCATGGTG 
AAACTAATCC ATGCAGATCC AAAGCTGTTG CTATGTAATC CAAGAAAACA AGGGCCTGAA 
ACTCAGGGGA GTACAGCAGA GTTAATCACA GGCCTTGTGC AGCTCGTCCC ACAGTCCAGC 
ATGCCAGAAA TAGCTCAAGA AGCAATGGAG GCTTTGCTGG TTCTTCATCA GTTGGATAGT 
ATTGACTTAT GGAATCCAGA TTCACCTATA GAAACATTCT GGGAGATTAG CTCACAAATG 
CTTTTTTATA TCTGTAAAAA GCTAACTACC CATCAGATGC TTAGCAGTAC AGAAATTCTC 
AAGTGGTTGC GAGAAATTTT GATCTGTAGG AACAAATATC TTCTTAAAAA TAAGGACGGT 
GCAATCGGGT CCAGCGGGAC GCCCATCTGC CGCCAGGCCC AGACTAAGCT GGAGGTTGCT 
CTGTACATGT TTCTGTGGAG TCCCGACATT GAAGCCGTGC TCGTGGCCAT GTCCTGCTTT 
CGACACCTGT GTGAGGAAGC CGACATCAGG TGTGGCATAG ATGAGATCTC GGTGCACCAT 
TTCTTGCCAA ACTATAACAC CTTCATGGAG TTCGCATCTG TCAGCAACAT GATGTCGACA 
GGAAGAGCAG CTCTTCAGAA AAGAGTGATG GCCCTGCTCA GGCGCATCGA ACACCCCACC 
GCTGGAAACA CCGAGGCTTG GGAAGATACT TACGCCAAAT GGGAGCAAGC TACAAAATCA 
ATCCTCAATT ATCCGAAAGC CAAAATTGAA GATGGCCAGG TTGCTGAGAG TCTTCACAAG 
ACCATTGTGA AGAGAAGAAT GTCTCACGTG AGTGGAGGGG GCTCCATAGA CTTGTGCGAC 
ACTGACTCGC TACAGGAGTG GATCAACATG ACTGGGTTTC TCTGTGCCCT TGGTGGCGTG 
TGCCTACAGC AGCGGAGCAA TGCCGGCTTT GCCACCTACA GCCCTCCCAT GGGCCCTATC 
AGTGAACGCA AGGGCTCTCT CATTTCCATG GTGTCCAGCG ATGGAAACGC AGAGACGCCT 
GTCAGCAAGT TCATGGATCG GCTGCTCTCC TTGATGGTGT GTAACCACGA AAAAGTGGGG 
CTGCAGATAC GAAGCAATGT CAAGGACTTG TTGGGTCTTG AATTGAGTCC TGCCCTTTAT 
CCAATGCTGT TTAACAAATT AAAAAATACC ATCAGCAAGT TTTTTGATCC TCAAGGACAG 
GTTTTATTGA CTGACACCAA CACCCAGTTT GTGGAGCAGA CCATAGCCAT AATGAAGAAC 
TTGCTTGATA ATCATGCTGA GGGTAGTTCT GAACACATGG GACAAGCTAG CATCGAAACT 
ATGATGTTAA ATCTGGTCAG ATATGTTAGA GTGTTTGGGA ATTTGGTGCA GGCAATTCAG 
ATCAAAACTA AGCTGTGCCA GCTGGTTCAA GTAATGATGG AGAGGAGAGA TGACCTTTCA 
TTTTGCCAAG AGATGAAATT TAGGAATAAA ATGGTAGAAT ATCTGACAGA TTGGGTCATG 
GGAACATCTA ACCAAGCTGC AGATGAAGAT GTGAAATGTC TAACTAGAGA TTTGGACCAG 
GCAAGCATGG AAGCAGTGGT GTCATTGCTC GCTGGGCTCC CTCTGCAGCC TGAGGAAGGC 
GATGGGGTGG AATTGATGGA AGCCAAATCC CAGCTGTTCC TTAAGTATTT CACCCTCTTT 
ATGAACCTTC TTAATGACTG CAGTGAGGTC GAAGAAGAAA ATGTCCAGAT TGGGGGCAGG 
AAGCGCGGCA TGTCGCGAAG GCTGGCGTCC CTGCGCCACT GCACCGTGCT GGCAATGTCC 
AACCTGCTCA ATGCGAATGT AGACAGCGGC CTGATGCACT CTATAGTGCT AGGATTGGGT 
TACCACAAAG ATCTTCAGAC AAGAGCCACC TTCATGGAAG TCCTCACAAA AATCCTTCAG 
CAAGGCACAG AGTTTGATAC GCTCGCAGAA ACCGTGTTAG CTGATCGATT TGAGAGACTT 
GTTGAGTTGG TCACGATGAT GGGTGATCAA GGAGAGCTCC CCATAGCCAT GGCTCTGGCC 
AACGTGGTGC CTTGTTCTCA GTGGGATGAA CTTGCTCGAG TCCTTGTTAC TCTCTTTGAT 
TCCCGACACT TACTGTATCA GCTCCTTTGG AACATGTTTT CTAAGGAAGT TGAGCTGGCA 
GACTCCATGC AGACACTTTT TAGAGGAAAC AGCTTGGCCA GTAAAATCAT GACCTTCTGC 
TTTAAGGTCT ACGGTGCTAC ATACCTGCAG AAGCTTCTGG AGCCTTTACT CCGCTTTGTG 
ATCACATCCT CCGAATGGCA GCACGTCAGC TTCGAGGTCG ATCCCACCAG GTTAGAGCAT 
ACTGAGAGCC TCGAGGACAA CCAGCGGAAC CTTTTGCAAA TGACTGAGAA GTTCTTCCAC 
GCCATCATCA GTTCTTCCTC CGAGTTCCCC CCTCAGCTAC GCAGTGTGTG CCACTGCCTG 
TACCAGGCAA CTTGCCACTC TCTACTGAAT AAAGCTACCG TAAAAGAAAA AAAGGAAAAC 
AAAAAATCAG TTGTTAGCCA GAGATTCCCT CAGAACAGCA TTGGCGCCGT GGGGAGTGCC 
ATGTTCCTGA GATTCATCAA CCCTGCCGTT GTCTCTCCCT ACGAGGCAGG GATTTTAGAG 
AGAAAACCGC TCCCTAGAAT TGAAAGGGGG TTGAAGCTGA TGTCAAAGAT TCTTCAGAGC 
ATTGCCAATC ATGTCCTTTT CACAAAAGAA GAGTATATGA GGCCTTTTAA CGATTTTGTG 
AAAAGCAACT TTGATGTAGC ACGGAGATTT TTCCTTGATA TCGCATCTGA TTGTCCTGTA 
AGTGATGCCA TAAACCACAG CCTTTCCTTC ATCAGCGATG GCAATGTCTT GGCTTTGCAC 
CGTCTGCTCT GGAACAACCA AGAGAAAATC GGCCAATATC TTTCAAGCAA CAGAGATCAC 
AAAGCTGTTG GAAGGCGACC TTTCGATAAG ATGGCAACAC TGCTTGCCTA CCTGGGACCT 
CCAGAGCACA AGCCTGTGGC TGACACACAC TGGTCCAGCC TAAATCTTAC CAGCTCCAAG 
TTTGAGGAAT TCATGACAAG GCACCAGGTA CATGAAAAAG AGGAATTCAA GGCTCTGAAA 
ACATTAAATA TCTTCTATCA AGCAGGCACT TCCAAAGCTG GAAATCCTGT TTTTTATTAT 
GTCACACGAA GGTTCAAGAC TGGCCAGATC AACGGGGATT TGCTTATATA CCACGTCTTA 
CTAACTTTAA AGCCATATTA TGCAAAGCCA TATGAGATTG TAATGGACCT CACTCATGCC 
GGGCCTAGCA ATCGCTTTAA GACAGACTTC CTCTCCAAGT GGTTTGTTGT TTTCCCGGGC 
TTTGCTTACG AGAACGTCTC TTCGGTCTAC ATCTACAACT GTAATTCTTG GGTTCGGGAG 
TACACCAAAT ACCATGAGAG ACTGCTCACG GGACTCAAAG GCAGCAAAAG ACTCTTTTTC 
ATAGACTCTC CCTCAAAACT GGCTGAGCAT ATCGATTATG ATCAGCAAAA ACTGCCAGCC 
GCGACCTTGG CTCTGGATGA AGACCTCAAG GTGTACAACA GTGCTCTCAA GCTGGCTCAC 
AAGGACACCA AGGTGACCCT CAAGGTTGGC TCTACTGCGA TCCAAGTGAC GTCATCTGAG 
CGAACTAAAG TCCTGGGACA GACGGTTTTT CTGAATGATA TTTACTATGC CTCTGAGATC 
GAAGAAATCT GTCTTGTGGA TGAAAACCAG TTCACTCTGA CCATTTCCAA TCAGGGCACT 
CCCCTCACCT TCATGCATCA AGAGTGTGAA GGCATTGTGG CGTCCATCAT TCACATCCGA 
ACTCGATGGG AGCTGTCCCA GCCAGATTCT ATCCCCCAGC ACACTAAGAT CCGGCCAAAG 
GACGTTCCCG GAACACTTTT GAATATCGCA TTACTTAACT TGGGTAGTTC GGACCCAAGT 
TTACGGTCAG CCGCCTATAA TCTTCTCTGT GCCTTAACCT GTACCTTTAA TTTAAAAATT 
GAGGGCCAAT TACTAGAAAC ATCAGGTCTG TGTATACCTG CCAACAATAC CCTTTTTATT 
GTCTCTATTA GTAAAACCCT GGCAGCTAAT GAGCCACACC TCACCTTAGA GTTTTTGGAA 
GAGTGCATTT CCGGATTTAG CAAATCCAGT ATTGAACTGA AGCACCTGTG TCTAGAATAC 
ATGACTCCCT GGCTATCAAA TCTAGTCCGT TTCTGTAAAC ATAATGATGA TGCCAAACGA 
CAGAGAGTCA CTGCTATTCT TGATAAACTT ATAACAATGA CCATAAATGA AAAGCAGATG 
TACCCATCGA TCCAAGCCAA GATATGGGGA AGCCTTGGGC AGATTACAGA TCTGCTCGAT 
GTAGTGCTGG ACAGCTTTAT CAAAACCAGT GCCACAGGTG GTTTAGGATC CATCAAGGCT 
GAAGTGATGG CTGACACGGC CGTGGCTCTG GCTTCTGGGA ACGTCAAACT AGTTTCGAGC 
AAGGTGATTG GAAGAATGTG CAAAATCATT GACAAGACCT GCCTCTCTCC AACTCCTACC 
TTAGAGCAGC ACCTCATGTG GGATGATATC GCCATTTTAG CACGCTACAT GCTAATGCTG 
TCCTTCAATA ATTCTCTGGA TGTGGCGGCT CATCTTCCCT ACCTCTTCCA TGTGGTGACT 
CTGTTGGTGG CCACAGGTCC CTTGTCCCTT CGTGCTTCCA CACACGGACT GGTCATCAAT 
ATCATCCATT CTCTGTGCAC GTGCTCACAG CTTCACTTTA GTGAAGAGAC CAAGCAAGTT 
TTACGACTGA GTCTGACTGA GTTCTCACTA CCTAAGTTTT ATTTGCTGTT CGGCATCAGC 
AAAGTCAAAT CAGCTGCCGT GATAGCCTTC CGGTCCAGTT ACCGGGACAG GTCTTTCTCC 
CCTGGCTCCT ATGAGAGGGA GACCTTTGCC CTGGCCTCCT TGGAAACAGT CACAGAAGCT 
CTCTTGGAGA TCATGGAGGC TTGTATGAGA GATATTCCTG CATGCAAGTG GCTGGACCAG 
TGGACAGAGC TAGCCCAGAG GTTTGCGTTC CAGTACAATC CCTCCCTGCA GCCACGAGCT 
CTGGTTGTGT TTGGTTGTAT CAGCAAAAGA GTGTCCCATG GGCAGATCAA GCAGATTATC 
CGCATTCTTA GCAAGGGACT TGAGAGCTGC CTAAAAGGAC CTGATAATTA TAACAGTCAA 
GTTCTGATTG AAGCTACCGT GATAGCTCTA ACCAAATTAC AACCACTTCT TAATAAGCAG 
GAGTCCCCTC TGCACCGAGC TCTCTTCTGG GTGGCCGTTG CCGTGCTGCA GCTGGATGAA 
GTGGACCTGT ACTCGGCGGG CACTGCCCTC CTCGAACAGA ACCTGCACAC CTTAGACAGC 
CTCCTGCACA TCTTCAACGA CCAGAGCCCA GAAGAAGTGT TCATGGAGAT CCGGCAGCCT 
CTGGAGTGGC ACTGCAAGCA GATGGACCAC TTTGTGGGGC TCAACTTTAA CTCCAGCTTT 
AACTTTGCCC TGGTCGGGCA CCTGCTGAAG GGATTTCGGC ATCCTTCACC TGTCACTGTT 
GCAAGAACTG TGAGGATTTT GCACACCCTA CTAGCATTGG TTAACAAGCA TAGGAATTGT 
GACAAGTTTG AGGTGAACAC CCAGAGTGTG GCTTACTTAG CAGCTTTACT TACTGTGTCT 
GAGGAGGTTC GAAGTCGCTG CAGCTTAAAG CATAGGAAAT CACTCCTACT AACTGATATC 
ACAATGGAAA ATGTGCCTAT GGATATATAT CCCATCTATC ACAGTGACCC CAGCCTTAGG 
ACACTAAAAG AAACACAACC GTGGTCCTCT CCAAAGGGAT CTGAGAGACA TCTTGCAGCC 
AGCTACCCTC CTGTGGGCCA AACTAGCCCC CGAACCAGAA AATCCATGAG CCTGGATATG 
GGGCAGCCTT CCCAGGCCAA CACAAAGAAG TTGCTTGGGA CAAGGAAAAG CTTCGATCAT 
TTGATCTCTG ATGCCAAAGC TCCCAAAAGG CAAGAACAGG AGTCTGGAGT CACCACGCCC 
CCCAAGATGC GGAGAGTGGC CGAGAACGAC TACGAGATGG AAACCCAGAG AATCTCCCCT 
CAGCCACACC CGCCTCTGCG GAAAGTGTCC GTGTCCGAAT CCAATGTGCT GCTGGACGAG 
GAAGTGCTCA CAGACCCCAA AACCCAGGCT CTTCTTCTCA CTGTCCTGGC TACTCTGGTG 
AAGTACACTG CGGACGAGTT TGACCAGCGC ATTCTCTATG AGTACTTAGC GGAGGCCAGT 
GTTGTCTTCC CAAAGGTTTT TCCAGTGGTA CATAATTTGT TGGACTCAAA GATAAATACT 
CTGCTCTCAT TATGCCAAGA CCCAAATTTG CTAAACCCGG TTCATGGAAT TGTGCAGAGT 
GTGGTCTACC ACGAAGAGGC CCCGCCGCAG TACCAGACGG CGTACCTCCA GAGTTTCGGT 
TTTAATGGTC TGTGGAGGTT TGCAGGACCA TTTTCTAAGC AGACGCAGAT CCCAGACTAT 
GCCGAGCTCA TCGTGAAGTT TCTCGATGCC TTGATTGACA CGTATCTGCC TGGAATTGAT 
GAGGAAGCCA GTGAGGAGTC CCTCCTGACA CCCACCTCTC CCTACCCGCC CGCGGTGCAG 
AGCCAGCTTA GCATCACTGC GAACCTCAAC CTCTCTAACT CCATGACCTC ACTGGCAACT 
TCCCAGCATT CCCCAGGCAT CGACAAGGAG AATGTGGAGC TCTCGCCAAC CACCGGGCAC 
TGCAGCGGCG GGAGGACGCG CCACGGCTCC GCCAGCCAGG TCCAGAAGCA AAGGAGTGCC 
GGCAGCTTCA AGAGGCACAG CATCAAGAAG ATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967656.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823516.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X17, mRNA.

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CloneID	OSa234704
Clone ID Related Accession (Same CDS sequence)	XM_031967656.1
Accession Version	XM_031967656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATAT TTGGTGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT AATTATTTTG 
GATACACTGG AAAAATGCCT TGCTGGGGTA AGTGATTTGC AGCCAAAGGA CACGATGAGG 
TTAGATGAAA CAATGCTGGT CAAACAGTTG CTGCCGGAAA TCTGCCATTT CCTTCACACC 
TACCGGGAAG GGAACCAGCA CGCAGCCGAG CTTCGGAGCT CCGCCTCTGG CGTCCTTTTC 
TCCCTCAGCT GTAATAACTT CAATGCCGTC TTCAGCCGGA TTTCCACCAG GTTACAGGAA 
TTGACTGTTT GCTCAGAAGA TAATGTTGAT GTCCATGATA TAGAATTATT ACAATATATC 
AATGTGGATT GTGCAAAACT AAAGCGGCTC TTGCAGGAAA CTGTATTTAA GTTTAAAGCC 
TTAAAGAAGG TAGCTCAACT AGCAGTTATA AATAGCCTAG AAAAGGCATT TTGGAATTGG 
GTAGAGAACT ATCCTGATGA ATTTACAAAG CTGTACCAGA CTCCCCAGAC AGATATGGCG 
GAATGTGCAG AAAAATTATT TGACTTGGTG GATGGTTTTG CTGAAAGCAC CAAACGTAAA 
GCTGCAGTTT GGCCACTGCA AATCATTCTT CTTGTCTTGT GTCCAGAAAT AATCCAGGAC 
ATTTCCAAGG ATGTGGTTGA TGAAGCGAAA ATGAATAAGT TTTCTACACT GTTGACCACT 
ATCACCGCCT GGATACTTTC TCCTTGGCTT TGTGATACCG ATGTCTCAAT TCCTCTTTCC 
TGCCAAATCA CTCGTCCTCA GTCCTCCTTT AAAATGTTTC TCGACAATCT CAGAAGAGCG 
CTGGCCGGCC ACAGTGGGAG CAGGCAGCTG ACAGAGAGTG CCGCGATCGC TTGTGTTAAA 
CTGTGCAAAG CCAGCACCTA CATCAACTGG GAGGATAACT CGGTCATTTT CCTGCTCGTC 
CAATCCATGG TGGTGGATCT GAAGAACTTG CTCTTTAATC CCAGTAAGCC CTTCTCCAGA 
GGCAGCCAGA ATGCTGATGT GGACCTCATG ATAGACTGCC TGGTTTCTTG CTTTCGCATC 
AGCCCTCACA ACAACCAACA CTTTAAGATC TGTTTGGCCC AGAACTCCCC ATCTACATTT 
CATTATGTGC TGGTAAACTC CCTCCATCGA ATTATCACCA ATAGCACTTT CAAGCATGGA 
CTTGGCACTG CTTCCGCCCT GGATTGGTGG CCCAAGATTG ATGCTGTGTA TTGCCATTCT 
GTAGAACTTC GAAACATGTT TGGTGAAACG CTTCACAAGG CCGTGCAGGG CTGTGGGCCT 
CACCCCGCGA TACGAATGGC GCCGAGTCTT ACATTTAAAG AAAAAATGAC AAGCCTTAAA 
TTTAAAGAAA AACCTACGGA CTTGGAAACA AGAAGTTATA AATACCTCTT ATTATCCATG 
GTGAAACTAA TCCATGCAGA TCCAAAGCTG TTGCTATGTA ATCCAAGAAA ACAAGGGCCT 
GAAACTCAGG GGAGTACAGC AGAGTTAATC ACAGGCCTTG TGCAGCTCGT CCCACAGTCC 
AGCATGCCAG AAATAGCTCA AGAAGCAATG GAGGCTTTGC TGGTTCTTCA TCAGTTGGAT 
AGTATTGACT TATGGAATCC AGATTCACCT ATAGAAACAT TCTGGGAGAT TAGCTCACAA 
ATGCTTTTTT ATATCTGTAA AAAGCTAACT ACCCATCAGA TGCTTAGCAG TACAGAAATT 
CTCAAGTGGT TGCGAGAAAT TTTGATCTGT AGGAACAAAT ATCTTCTTAA AAATAAGCAG 
TCAGATAGGA GTTCTTCCCA TTTCCTCTAC CTTTATGGGG CAGGCTATGA TGTCTCTTCT 
GGTGGAGGTA CCAGTCATAT CTCTCTAGAC CACGAGGAGT TTATGCGTAC CTGTGCTCAT 
GGACCATCTC TCAGGAAAGT GAAAGGAAGC TCCTCAATGG ACGGTGCAAT CGGGTCCAGC 
GGGACGCCCA TCTGCCGCCA GGCCCAGACT AAGCTGGAGG TTGCTCTGTA CATGTTTCTG 
TGGAGTCCCG ACATTGAAGC CGTGCTCGTG GCCATGTCCT GCTTTCGACA CCTGTGTGAG 
GAAGCCGACA TCAGGTGTGG CATAGATGAG ATCTCGGTGC ACCATTTCTT GCCAAACTAT 
AACACCTTCA TGGAGTTCGC ATCTGTCAGC AACATGATGT CGACAGGAAG AGCAGCTCTT 
CAGAAAAGAG TGATGGCCCT GCTCAGGCGC ATCGAACACC CCACCGCTGG AAACACCGAG 
GCTTGGGAAG ATACTTACGC CAAATGGGAG CAAGCTACAA AATCAATCCT CAATTATCCG 
AAAGCCAAAA TTGAAGATGG CCAGGTTGCT GAGAGTCTTC ACAAGACCAT TGTGAAGAGA 
AGAATGTCTC ACGTGAGTGG AGGGGGCTCC ATAGACTTGT GCGACACTGA CTCGCTACAG 
GAGTGGATCA ACATGACTGG GTTTCTCTGT GCCCTTGGTG GCGTGTGCCT ACAGCAGCGG 
AGCAATGCCG GCTTTGCCAC CTACAGCCCT CCCATGGGCC CTATCAGTGA ACGCAAGGGC 
TCTCTCATTT CCATGGTGTC CAGCGATGGA AACGCAGAGA CGCCTGTCAG CAAGTTCATG 
GATCGGCTGC TCTCCTTGAT GGTGTGTAAC CACGAAAAAG TGGGGCTGCA GATACGAAGC 
AATGTCAAGG ACTTGTTGGG TCTTGAATTG AGTCCTGCCC TTTATCCAAT GCTGTTTAAC 
AAATTAAAAA ATACCATCAG CAAGTTTTTT GATCCTCAAG GACAGGTTTT ATTGACTGAC 
ACCAACACCC AGTTTGTGGA GCAGACCATA GCCATAATGA AGAACTTGCT TGATAATCAT 
GCTGAGGGTA GTTCTGAACA CATGGGACAA GCTAGCATCG AAACTATGAT GTTAAATCTG 
GTCAGATATG TTAGAGTGTT TGGGAATTTG GTGCAGGCAA TTCAGATCAA AACTAAGCTG 
TGCCAGCTGG TTCAAGTAAT GATGGAGAGG AGAGATGACC TTTCATTTTG CCAAGAGATG 
AAATTTAGGA ATAAAATGGT AGAATATCTG ACAGATTGGG TCATGGGAAC ATCTAACCAA 
GCTGCAGATG AAGATGTGAA ATGTCTAACT AGAGATTTGG ACCAGGCAAG CATGGAAGCA 
GTGGTGTCAT TGCTCGCTGG GCTCCCTCTG CAGCCTGAGG AAGGCGATGG GGTGGAATTG 
ATGGAAGCCA AATCCCAGCT GTTCCTTAAG TATTTCACCC TCTTTATGAA CCTTCTTAAT 
GACTGCAGTG AGGTCGAAGA AGAAAATGTC CAGATTGGGG GCAGGAAGCG CGGCATGTCG 
CGAAGGCTGG CGTCCCTGCG CCACTGCACC GTGCTGGCAA TGTCCAACCT GCTCAATGCG 
AATGTAGACA GCGGCCTGAT GCACTCTATA GTGCTAGGAT TGGGTTACCA CAAAGATCTT 
CAGACAAGAG CCACCTTCAT GGAAGTCCTC ACAAAAATCC TTCAGCAAGG CACAGAGTTT 
GATACGCTCG CAGAAACCGT GTTAGCTGAT CGATTTGAGA GACTTGTTGA GTTGGTCACG 
ATGATGGGTG ATCAAGGAGA GCTCCCCATA GCCATGGCTC TGGCCAACGT GGTGCCTTGT 
TCTCAGTGGG ATGAACTTGC TCGAGTCCTT GTTACTCTCT TTGATTCCCG ACACTTACTG 
TATCAGCTCC TTTGGAACAT GTTTTCTAAG GAAGTTGAGC TGGCAGACTC CATGCAGACA 
CTTTTTAGAG GAAACAGCTT GGCCAGTAAA ATCATGACCT TCTGCTTTAA GGTCTACGGT 
GCTACATACC TGCAGAAGCT TCTGGAGCCT TTACTCCGCT TTGTGATCAC ATCCTCCGAA 
TGGCAGCACG TCAGCTTCGA GGTCGATCCC ACCAGGTTAG AGCATACTGA GAGCCTCGAG 
GACAACCAGC GGAACCTTTT GCAAATGACT GAGAAGTTCT TCCACGCCAT CATCAGTTCT 
TCCTCCGAGT TCCCCCCTCA GCTACGCAGT GTGTGCCACT GCCTGTACCA GGCAACTTGC 
CACTCTCTAC TGAATAAAGC TACCGTAAAA GAAAAAAAGG AAAACAAAAA ATCAGTTGTT 
AGCCAGAGAT TCCCTCAGAA CAGCATTGGC GCCGTGGGGA GTGCCATGTT CCTGAGATTC 
ATCAACCCTG CCGTTGTCTC TCCCTACGAG GCAGGGATTT TAGAGAGAAA ACCGCTCCCT 
AGAATTGAAA GGGGGTTGAA GCTGATGTCA AAGATTCTTC AGAGCATTGC CAATCATGTC 
CTTTTCACAA AAGAAGAGTA TATGAGGCCT TTTAACGATT TTGTGAAAAG CAACTTTGAT 
GTAGCACGGA GATTTTTCCT TGATATCGCA TCTGATTGTC CTGTAAGTGA TGCCATAAAC 
CACAGCCTTT CCTTCATCAG CGATGGCAAT GTCTTGGCTT TGCACCGTCT GCTCTGGAAC 
AACCAAGAGA AAATCGGCCA ATATCTTTCA AGCAACAGAG ATCACAAAGC TGTTGGAAGG 
CGACCTTTCG ATAAGATGGC AACACTGCTT GCCTACCTGG GACCTCCAGA GCACAAGCCT 
GTGGCTGACA CACACTGGTC CAGCCTAAAT CTTACCAGCT CCAAGTTTGA GGAATTCATG 
ACAAGGCACC AGGTACATGA AAAAGAGGAA TTCAAGGCTC TGAAAACATT AAATATCTTC 
TATCAAGCAG GCACTTCCAA AGCTGGAAAT CCTGTTTTTT ATTATGTCAC ACGAAGGTTC 
AAGACTGGCC AGATCAACGG GGATTTGCTT ATATACCACG TCTTACTAAC TTTAAAGCCA 
TATTATGCAA AGCCATATGA GATTGTAATG GACCTCACTC ATGCCGGGCC TAGCAATCGC 
TTTAAGACAG ACTTCCTCTC CAAGTGGTTT GTTGTTTTCC CGGGCTTTGC TTACGAGAAC 
GTCTCTTCGG TCTACATCTA CAACTGTAAT TCTTGGGTTC GGGAGTACAC CAAATACCAT 
GAGAGACTGC TCACGGGACT CAAAGGCAGC AAAAGACTCT TTTTCATAGA CTCTCCCTCA 
AAACTGGCTG AGCATATCGA TTATGATCAG CAAAAACTGC CAGCCGCGAC CTTGGCTCTG 
GATGAAGACC TCAAGGTGTA CAACAGTGCT CTCAAGCTGG CTCACAAGGA CACCAAGGTG 
ACCCTCAAGG TTGGCTCTAC TGCGATCCAA GTGACGTCAT CTGAGCGAAC TAAAGTCCTG 
GGACAGACGG TTTTTCTGAA TGATATTTAC TATGCCTCTG AGATCGAAGA AATCTGTCTT 
GTGGATGAAA ACCAGTTCAC TCTGACCATT TCCAATCAGG GCACTCCCCT CACCTTCATG 
CATCAAGAGT GTGAAGGCAT TGTGGCGTCC ATCATTCACA TCCGAACTCG ATGGGAGCTG 
TCCCAGCCAG ATTCTATCCC CCAGCACACT AAGATCCGGC CAAAGGACGT TCCCGGAACA 
CTTTTGAATA TCGCATTACT TAACTTGGGT AGTTCGGACC CAAGTTTACG GTCAGCCGCC 
TATAATCTTC TCTGTGCCTT AACCTGTACC TTTAATTTAA AAATTGAGGG CCAATTACTA 
GAAACATCAG GTCTGTGTAT ACCTGCCAAC AATACCCTTT TTATTGTCTC TATTAGTAAA 
ACCCTGGCAG CTAATGAGCC ACACCTCACC TTAGAGTTTT TGGAAGAGTG CATTTCCGGA 
TTTAGCAAAT CCAGTATTGA ACTGAAGCAC CTGTGTCTAG AATACATGAC TCCCTGGCTA 
TCAAATCTAG TCCGTTTCTG TAAACATAAT GATGATGCCA AACGACAGAG AGTCACTGCT 
ATTCTTGATA AACTTATAAC AATGACCATA AATGAAAAGC AGATGTACCC ATCGATCCAA 
GCCAAGATAT GGGGAAGCCT TGGGCAGATT ACAGATCTGC TCGATGTAGT GCTGGACAGC 
TTTATCAAAA CCAGTGCCAC AGGTGGTTTA GGATCCATCA AGGCTGAAGT GATGGCTGAC 
ACGGCCGTGG CTCTGGCTTC TGGGAACGTC AAACTAGTTT CGAGCAAGGT GATTGGAAGA 
ATGTGCAAAA TCATTGACAA GACCTGCCTC TCTCCAACTC CTACCTTAGA GCAGCACCTC 
ATGTGGGATG ATATCGCCAT TTTAGCACGC TACATGCTAA TGCTGTCCTT CAATAATTCT 
CTGGATGTGG CGGCTCATCT TCCCTACCTC TTCCATGTGG TGACTCTGTT GGTGGCCACA 
GGTCCCTTGT CCCTTCGTGC TTCCACACAC GGACTGGTCA TCAATATCAT CCATTCTCTG 
TGCACGTGCT CACAGCTTCA CTTTAGTGAA GAGACCAAGC AAGTTTTACG ACTGAGTCTG 
ACTGAGTTCT CACTACCTAA GTTTTATTTG CTGTTCGGCA TCAGCAAAGT CAAATCAGCT 
GCCGTGATAG CCTTCCGGTC CAGTTACCGG GACAGGTCTT TCTCCCCTGG CTCCTATGAG 
AGGGAGACCT TTGCCCTGGC CTCCTTGGAA ACAGTCACAG AAGCTCTCTT GGAGATCATG 
GAGGCTTGTA TGAGAGATAT TCCTGCATGC AAGTGGCTGG ACCAGTGGAC AGAGCTAGCC 
CAGAGGTTTG CGTTCCAGTA CAATCCCTCC CTGCAGCCAC GAGCTCTGGT TGTGTTTGGT 
TGTATCAGCA AAAGAGTGTC CCATGGGCAG ATCAAGCAGA TTATCCGCAT TCTTAGCAAG 
GGACTTGAGA GCTGCCTAAA AGGACCTGAT AATTATAACA GTCAAGTTCT GATTGAAGCT 
ACCGTGATAG CTCTAACCAA ATTACAACCA CTTCTTAATA AGCAGGAGTC CCCTCTGCAC 
CGAGCTCTCT TCTGGGTGGC CGTTGCCGTG CTGCAGCTGG ATGAAGTGGA CCTGTACTCG 
GCGGGCACTG CCCTCCTCGA ACAGAACCTG CACACCTTAG ACAGCCTCCT GCACATCTTC 
AACGACCAGA GCCCAGAAGA AGTGTTCATG GAGATCCGGC AGCCTCTGGA GTGGCACTGC 
AAGCAGATGG ACCACTTTGT GGGGCTCAAC TTTAACTCCA GCTTTAACTT TGCCCTGGTC 
GGGCACCTGC TGAAGGGATT TCGGCATCCT TCACCTGTCA CTGTTGCAAG AACTGTGAGG 
ATTTTGCACA CCCTACTAGC ATTGGTTAAC AAGCATAGGA ATTGTGACAA GTTTGAGGTG 
AACACCCAGA GTGTGGCTTA CTTAGCAGCT TTACTTACTG TGTCTGAGGA GGTTCGAAGT 
CGCTGCAGCT TAAAGCATAG GAAATCACTC CTACTAACTG ATATCACAAT GGAAAATGTG 
CCTATGGATA TATATCCCAT CTATCACAGT GACCCCAGCC TTAGGACACT AAAAGAAACA 
CAACCGTGGT CCTCTCCAAA GGGATCTGAG AGACATCTTG CAGCCAGCTA CCCTCCTGTG 
GGCCAAACTA GCCCCCGAAC CAGAAAATCC ATGAGCCTGG ATATGGGGCA GCCTTCCCAG 
GCCAACACAA AGAAGTTGCT TGGGACAAGG AAAAGCTTCG ATCATTTGAT CTCTGATGCC 
AAAGCTCCCA AAAGGCAAGA ACAGGAGTCT GGAGTCACCA CGCCCCCCAA GATGCGGAGA 
GTGGCCGAGA ACGACTACGA GATGGAAACC CAGAGAATCT CCCCTCAGCC ACACCCGCCT 
CTGCGGAAAG TGTCCGTGTC CGAATCCAAT GTGCTGCTGG ACGAGGAAGT GCTCACAGAC 
CCCAAAACCC AGGCTCTTCT TCTCACTGTC CTGGCTACTC TGGTGAAGTA CACTGCGGAC 
GAGTTTGACC AGCGCATTCT CTATGAGTAC TTAGCGGAGG CCAGTGTTGT CTTCCCAAAG 
GTTTTTCCAG TGGTACATAA TTTGTTGGAC TCAAAGATAA ATACTCTGCT CTCATTATGC 
CAAGACCCAA ATTTGCTAAA CCCGGTTCAT GGAATTGTGC AGAGTGTGGT CTACCACGAA 
GAGGCCCCGC CGCAGTACCA GACGGCGTAC CTCCAGAGTT TCGGTTTTAA TGGTCTGTGG 
AGGTTTGCAG GACCATTTTC TAAGCAGACG CAGATCCCAG ACTATGCCGA GCTCATCGTG 
AAGTTTCTCG ATGCCTTGAT TGACACGTAT CTGCCTGGAA TTGATGAGGA AGCCAGTGAG 
GAGTCCCTCC TGACACCCAC CTCTCCCTAC CCGCCCGCGG TGCAGAGCCA GCTTAGCATC 
ACTGCGAACC TCAACCTCTC TAACTCCATG ACCTCACTGG CAACTTCCCA GCATTCCCCA 
GGCATCGACA AGGAGAATGT GGAGCTCTCG CCAACCACCG GGCACTGCAG CGGCGGGAGG 
ACGCGCCACG GCTCCGCCAG CCAGGTCCAG AAGCAAAGGA GTGCCGGCAG CTTCAAGAGG 
CACAGCATCA AGAAGATCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823516.1
CDS	264..8747
Translation	MRIFGEAAEKNLYLSQLIILDTLEKCLAGVSDLQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKFSTLLTTITAWILSPWLCDTDVSIPLSCQITRPQSSFKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSTFKHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIVLGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQATCHSLLNKATVKEKKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKQESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967656.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967656.1

ORF Insert Sequence:

ATGAGAATAT TTGGTGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT AATTATTTTG 
GATACACTGG AAAAATGCCT TGCTGGGGTA AGTGATTTGC AGCCAAAGGA CACGATGAGG 
TTAGATGAAA CAATGCTGGT CAAACAGTTG CTGCCGGAAA TCTGCCATTT CCTTCACACC 
TACCGGGAAG GGAACCAGCA CGCAGCCGAG CTTCGGAGCT CCGCCTCTGG CGTCCTTTTC 
TCCCTCAGCT GTAATAACTT CAATGCCGTC TTCAGCCGGA TTTCCACCAG GTTACAGGAA 
TTGACTGTTT GCTCAGAAGA TAATGTTGAT GTCCATGATA TAGAATTATT ACAATATATC 
AATGTGGATT GTGCAAAACT AAAGCGGCTC TTGCAGGAAA CTGTATTTAA GTTTAAAGCC 
TTAAAGAAGG TAGCTCAACT AGCAGTTATA AATAGCCTAG AAAAGGCATT TTGGAATTGG 
GTAGAGAACT ATCCTGATGA ATTTACAAAG CTGTACCAGA CTCCCCAGAC AGATATGGCG 
GAATGTGCAG AAAAATTATT TGACTTGGTG GATGGTTTTG CTGAAAGCAC CAAACGTAAA 
GCTGCAGTTT GGCCACTGCA AATCATTCTT CTTGTCTTGT GTCCAGAAAT AATCCAGGAC 
ATTTCCAAGG ATGTGGTTGA TGAAGCGAAA ATGAATAAGT TTTCTACACT GTTGACCACT 
ATCACCGCCT GGATACTTTC TCCTTGGCTT TGTGATACCG ATGTCTCAAT TCCTCTTTCC 
TGCCAAATCA CTCGTCCTCA GTCCTCCTTT AAAATGTTTC TCGACAATCT CAGAAGAGCG 
CTGGCCGGCC ACAGTGGGAG CAGGCAGCTG ACAGAGAGTG CCGCGATCGC TTGTGTTAAA 
CTGTGCAAAG CCAGCACCTA CATCAACTGG GAGGATAACT CGGTCATTTT CCTGCTCGTC 
CAATCCATGG TGGTGGATCT GAAGAACTTG CTCTTTAATC CCAGTAAGCC CTTCTCCAGA 
GGCAGCCAGA ATGCTGATGT GGACCTCATG ATAGACTGCC TGGTTTCTTG CTTTCGCATC 
AGCCCTCACA ACAACCAACA CTTTAAGATC TGTTTGGCCC AGAACTCCCC ATCTACATTT 
CATTATGTGC TGGTAAACTC CCTCCATCGA ATTATCACCA ATAGCACTTT CAAGCATGGA 
CTTGGCACTG CTTCCGCCCT GGATTGGTGG CCCAAGATTG ATGCTGTGTA TTGCCATTCT 
GTAGAACTTC GAAACATGTT TGGTGAAACG CTTCACAAGG CCGTGCAGGG CTGTGGGCCT 
CACCCCGCGA TACGAATGGC GCCGAGTCTT ACATTTAAAG AAAAAATGAC AAGCCTTAAA 
TTTAAAGAAA AACCTACGGA CTTGGAAACA AGAAGTTATA AATACCTCTT ATTATCCATG 
GTGAAACTAA TCCATGCAGA TCCAAAGCTG TTGCTATGTA ATCCAAGAAA ACAAGGGCCT 
GAAACTCAGG GGAGTACAGC AGAGTTAATC ACAGGCCTTG TGCAGCTCGT CCCACAGTCC 
AGCATGCCAG AAATAGCTCA AGAAGCAATG GAGGCTTTGC TGGTTCTTCA TCAGTTGGAT 
AGTATTGACT TATGGAATCC AGATTCACCT ATAGAAACAT TCTGGGAGAT TAGCTCACAA 
ATGCTTTTTT ATATCTGTAA AAAGCTAACT ACCCATCAGA TGCTTAGCAG TACAGAAATT 
CTCAAGTGGT TGCGAGAAAT TTTGATCTGT AGGAACAAAT ATCTTCTTAA AAATAAGCAG 
TCAGATAGGA GTTCTTCCCA TTTCCTCTAC CTTTATGGGG CAGGCTATGA TGTCTCTTCT 
GGTGGAGGTA CCAGTCATAT CTCTCTAGAC CACGAGGAGT TTATGCGTAC CTGTGCTCAT 
GGACCATCTC TCAGGAAAGT GAAAGGAAGC TCCTCAATGG ACGGTGCAAT CGGGTCCAGC 
GGGACGCCCA TCTGCCGCCA GGCCCAGACT AAGCTGGAGG TTGCTCTGTA CATGTTTCTG 
TGGAGTCCCG ACATTGAAGC CGTGCTCGTG GCCATGTCCT GCTTTCGACA CCTGTGTGAG 
GAAGCCGACA TCAGGTGTGG CATAGATGAG ATCTCGGTGC ACCATTTCTT GCCAAACTAT 
AACACCTTCA TGGAGTTCGC ATCTGTCAGC AACATGATGT CGACAGGAAG AGCAGCTCTT 
CAGAAAAGAG TGATGGCCCT GCTCAGGCGC ATCGAACACC CCACCGCTGG AAACACCGAG 
GCTTGGGAAG ATACTTACGC CAAATGGGAG CAAGCTACAA AATCAATCCT CAATTATCCG 
AAAGCCAAAA TTGAAGATGG CCAGGTTGCT GAGAGTCTTC ACAAGACCAT TGTGAAGAGA 
AGAATGTCTC ACGTGAGTGG AGGGGGCTCC ATAGACTTGT GCGACACTGA CTCGCTACAG 
GAGTGGATCA ACATGACTGG GTTTCTCTGT GCCCTTGGTG GCGTGTGCCT ACAGCAGCGG 
AGCAATGCCG GCTTTGCCAC CTACAGCCCT CCCATGGGCC CTATCAGTGA ACGCAAGGGC 
TCTCTCATTT CCATGGTGTC CAGCGATGGA AACGCAGAGA CGCCTGTCAG CAAGTTCATG 
GATCGGCTGC TCTCCTTGAT GGTGTGTAAC CACGAAAAAG TGGGGCTGCA GATACGAAGC 
AATGTCAAGG ACTTGTTGGG TCTTGAATTG AGTCCTGCCC TTTATCCAAT GCTGTTTAAC 
AAATTAAAAA ATACCATCAG CAAGTTTTTT GATCCTCAAG GACAGGTTTT ATTGACTGAC 
ACCAACACCC AGTTTGTGGA GCAGACCATA GCCATAATGA AGAACTTGCT TGATAATCAT 
GCTGAGGGTA GTTCTGAACA CATGGGACAA GCTAGCATCG AAACTATGAT GTTAAATCTG 
GTCAGATATG TTAGAGTGTT TGGGAATTTG GTGCAGGCAA TTCAGATCAA AACTAAGCTG 
TGCCAGCTGG TTCAAGTAAT GATGGAGAGG AGAGATGACC TTTCATTTTG CCAAGAGATG 
AAATTTAGGA ATAAAATGGT AGAATATCTG ACAGATTGGG TCATGGGAAC ATCTAACCAA 
GCTGCAGATG AAGATGTGAA ATGTCTAACT AGAGATTTGG ACCAGGCAAG CATGGAAGCA 
GTGGTGTCAT TGCTCGCTGG GCTCCCTCTG CAGCCTGAGG AAGGCGATGG GGTGGAATTG 
ATGGAAGCCA AATCCCAGCT GTTCCTTAAG TATTTCACCC TCTTTATGAA CCTTCTTAAT 
GACTGCAGTG AGGTCGAAGA AGAAAATGTC CAGATTGGGG GCAGGAAGCG CGGCATGTCG 
CGAAGGCTGG CGTCCCTGCG CCACTGCACC GTGCTGGCAA TGTCCAACCT GCTCAATGCG 
AATGTAGACA GCGGCCTGAT GCACTCTATA GTGCTAGGAT TGGGTTACCA CAAAGATCTT 
CAGACAAGAG CCACCTTCAT GGAAGTCCTC ACAAAAATCC TTCAGCAAGG CACAGAGTTT 
GATACGCTCG CAGAAACCGT GTTAGCTGAT CGATTTGAGA GACTTGTTGA GTTGGTCACG 
ATGATGGGTG ATCAAGGAGA GCTCCCCATA GCCATGGCTC TGGCCAACGT GGTGCCTTGT 
TCTCAGTGGG ATGAACTTGC TCGAGTCCTT GTTACTCTCT TTGATTCCCG ACACTTACTG 
TATCAGCTCC TTTGGAACAT GTTTTCTAAG GAAGTTGAGC TGGCAGACTC CATGCAGACA 
CTTTTTAGAG GAAACAGCTT GGCCAGTAAA ATCATGACCT TCTGCTTTAA GGTCTACGGT 
GCTACATACC TGCAGAAGCT TCTGGAGCCT TTACTCCGCT TTGTGATCAC ATCCTCCGAA 
TGGCAGCACG TCAGCTTCGA GGTCGATCCC ACCAGGTTAG AGCATACTGA GAGCCTCGAG 
GACAACCAGC GGAACCTTTT GCAAATGACT GAGAAGTTCT TCCACGCCAT CATCAGTTCT 
TCCTCCGAGT TCCCCCCTCA GCTACGCAGT GTGTGCCACT GCCTGTACCA GGCAACTTGC 
CACTCTCTAC TGAATAAAGC TACCGTAAAA GAAAAAAAGG AAAACAAAAA ATCAGTTGTT 
AGCCAGAGAT TCCCTCAGAA CAGCATTGGC GCCGTGGGGA GTGCCATGTT CCTGAGATTC 
ATCAACCCTG CCGTTGTCTC TCCCTACGAG GCAGGGATTT TAGAGAGAAA ACCGCTCCCT 
AGAATTGAAA GGGGGTTGAA GCTGATGTCA AAGATTCTTC AGAGCATTGC CAATCATGTC 
CTTTTCACAA AAGAAGAGTA TATGAGGCCT TTTAACGATT TTGTGAAAAG CAACTTTGAT 
GTAGCACGGA GATTTTTCCT TGATATCGCA TCTGATTGTC CTGTAAGTGA TGCCATAAAC 
CACAGCCTTT CCTTCATCAG CGATGGCAAT GTCTTGGCTT TGCACCGTCT GCTCTGGAAC 
AACCAAGAGA AAATCGGCCA ATATCTTTCA AGCAACAGAG ATCACAAAGC TGTTGGAAGG 
CGACCTTTCG ATAAGATGGC AACACTGCTT GCCTACCTGG GACCTCCAGA GCACAAGCCT 
GTGGCTGACA CACACTGGTC CAGCCTAAAT CTTACCAGCT CCAAGTTTGA GGAATTCATG 
ACAAGGCACC AGGTACATGA AAAAGAGGAA TTCAAGGCTC TGAAAACATT AAATATCTTC 
TATCAAGCAG GCACTTCCAA AGCTGGAAAT CCTGTTTTTT ATTATGTCAC ACGAAGGTTC 
AAGACTGGCC AGATCAACGG GGATTTGCTT ATATACCACG TCTTACTAAC TTTAAAGCCA 
TATTATGCAA AGCCATATGA GATTGTAATG GACCTCACTC ATGCCGGGCC TAGCAATCGC 
TTTAAGACAG ACTTCCTCTC CAAGTGGTTT GTTGTTTTCC CGGGCTTTGC TTACGAGAAC 
GTCTCTTCGG TCTACATCTA CAACTGTAAT TCTTGGGTTC GGGAGTACAC CAAATACCAT 
GAGAGACTGC TCACGGGACT CAAAGGCAGC AAAAGACTCT TTTTCATAGA CTCTCCCTCA 
AAACTGGCTG AGCATATCGA TTATGATCAG CAAAAACTGC CAGCCGCGAC CTTGGCTCTG 
GATGAAGACC TCAAGGTGTA CAACAGTGCT CTCAAGCTGG CTCACAAGGA CACCAAGGTG 
ACCCTCAAGG TTGGCTCTAC TGCGATCCAA GTGACGTCAT CTGAGCGAAC TAAAGTCCTG 
GGACAGACGG TTTTTCTGAA TGATATTTAC TATGCCTCTG AGATCGAAGA AATCTGTCTT 
GTGGATGAAA ACCAGTTCAC TCTGACCATT TCCAATCAGG GCACTCCCCT CACCTTCATG 
CATCAAGAGT GTGAAGGCAT TGTGGCGTCC ATCATTCACA TCCGAACTCG ATGGGAGCTG 
TCCCAGCCAG ATTCTATCCC CCAGCACACT AAGATCCGGC CAAAGGACGT TCCCGGAACA 
CTTTTGAATA TCGCATTACT TAACTTGGGT AGTTCGGACC CAAGTTTACG GTCAGCCGCC 
TATAATCTTC TCTGTGCCTT AACCTGTACC TTTAATTTAA AAATTGAGGG CCAATTACTA 
GAAACATCAG GTCTGTGTAT ACCTGCCAAC AATACCCTTT TTATTGTCTC TATTAGTAAA 
ACCCTGGCAG CTAATGAGCC ACACCTCACC TTAGAGTTTT TGGAAGAGTG CATTTCCGGA 
TTTAGCAAAT CCAGTATTGA ACTGAAGCAC CTGTGTCTAG AATACATGAC TCCCTGGCTA 
TCAAATCTAG TCCGTTTCTG TAAACATAAT GATGATGCCA AACGACAGAG AGTCACTGCT 
ATTCTTGATA AACTTATAAC AATGACCATA AATGAAAAGC AGATGTACCC ATCGATCCAA 
GCCAAGATAT GGGGAAGCCT TGGGCAGATT ACAGATCTGC TCGATGTAGT GCTGGACAGC 
TTTATCAAAA CCAGTGCCAC AGGTGGTTTA GGATCCATCA AGGCTGAAGT GATGGCTGAC 
ACGGCCGTGG CTCTGGCTTC TGGGAACGTC AAACTAGTTT CGAGCAAGGT GATTGGAAGA 
ATGTGCAAAA TCATTGACAA GACCTGCCTC TCTCCAACTC CTACCTTAGA GCAGCACCTC 
ATGTGGGATG ATATCGCCAT TTTAGCACGC TACATGCTAA TGCTGTCCTT CAATAATTCT 
CTGGATGTGG CGGCTCATCT TCCCTACCTC TTCCATGTGG TGACTCTGTT GGTGGCCACA 
GGTCCCTTGT CCCTTCGTGC TTCCACACAC GGACTGGTCA TCAATATCAT CCATTCTCTG 
TGCACGTGCT CACAGCTTCA CTTTAGTGAA GAGACCAAGC AAGTTTTACG ACTGAGTCTG 
ACTGAGTTCT CACTACCTAA GTTTTATTTG CTGTTCGGCA TCAGCAAAGT CAAATCAGCT 
GCCGTGATAG CCTTCCGGTC CAGTTACCGG GACAGGTCTT TCTCCCCTGG CTCCTATGAG 
AGGGAGACCT TTGCCCTGGC CTCCTTGGAA ACAGTCACAG AAGCTCTCTT GGAGATCATG 
GAGGCTTGTA TGAGAGATAT TCCTGCATGC AAGTGGCTGG ACCAGTGGAC AGAGCTAGCC 
CAGAGGTTTG CGTTCCAGTA CAATCCCTCC CTGCAGCCAC GAGCTCTGGT TGTGTTTGGT 
TGTATCAGCA AAAGAGTGTC CCATGGGCAG ATCAAGCAGA TTATCCGCAT TCTTAGCAAG 
GGACTTGAGA GCTGCCTAAA AGGACCTGAT AATTATAACA GTCAAGTTCT GATTGAAGCT 
ACCGTGATAG CTCTAACCAA ATTACAACCA CTTCTTAATA AGCAGGAGTC CCCTCTGCAC 
CGAGCTCTCT TCTGGGTGGC CGTTGCCGTG CTGCAGCTGG ATGAAGTGGA CCTGTACTCG 
GCGGGCACTG CCCTCCTCGA ACAGAACCTG CACACCTTAG ACAGCCTCCT GCACATCTTC 
AACGACCAGA GCCCAGAAGA AGTGTTCATG GAGATCCGGC AGCCTCTGGA GTGGCACTGC 
AAGCAGATGG ACCACTTTGT GGGGCTCAAC TTTAACTCCA GCTTTAACTT TGCCCTGGTC 
GGGCACCTGC TGAAGGGATT TCGGCATCCT TCACCTGTCA CTGTTGCAAG AACTGTGAGG 
ATTTTGCACA CCCTACTAGC ATTGGTTAAC AAGCATAGGA ATTGTGACAA GTTTGAGGTG 
AACACCCAGA GTGTGGCTTA CTTAGCAGCT TTACTTACTG TGTCTGAGGA GGTTCGAAGT 
CGCTGCAGCT TAAAGCATAG GAAATCACTC CTACTAACTG ATATCACAAT GGAAAATGTG 
CCTATGGATA TATATCCCAT CTATCACAGT GACCCCAGCC TTAGGACACT AAAAGAAACA 
CAACCGTGGT CCTCTCCAAA GGGATCTGAG AGACATCTTG CAGCCAGCTA CCCTCCTGTG 
GGCCAAACTA GCCCCCGAAC CAGAAAATCC ATGAGCCTGG ATATGGGGCA GCCTTCCCAG 
GCCAACACAA AGAAGTTGCT TGGGACAAGG AAAAGCTTCG ATCATTTGAT CTCTGATGCC 
AAAGCTCCCA AAAGGCAAGA ACAGGAGTCT GGAGTCACCA CGCCCCCCAA GATGCGGAGA 
GTGGCCGAGA ACGACTACGA GATGGAAACC CAGAGAATCT CCCCTCAGCC ACACCCGCCT 
CTGCGGAAAG TGTCCGTGTC CGAATCCAAT GTGCTGCTGG ACGAGGAAGT GCTCACAGAC 
CCCAAAACCC AGGCTCTTCT TCTCACTGTC CTGGCTACTC TGGTGAAGTA CACTGCGGAC 
GAGTTTGACC AGCGCATTCT CTATGAGTAC TTAGCGGAGG CCAGTGTTGT CTTCCCAAAG 
GTTTTTCCAG TGGTACATAA TTTGTTGGAC TCAAAGATAA ATACTCTGCT CTCATTATGC 
CAAGACCCAA ATTTGCTAAA CCCGGTTCAT GGAATTGTGC AGAGTGTGGT CTACCACGAA 
GAGGCCCCGC CGCAGTACCA GACGGCGTAC CTCCAGAGTT TCGGTTTTAA TGGTCTGTGG 
AGGTTTGCAG GACCATTTTC TAAGCAGACG CAGATCCCAG ACTATGCCGA GCTCATCGTG 
AAGTTTCTCG ATGCCTTGAT TGACACGTAT CTGCCTGGAA TTGATGAGGA AGCCAGTGAG 
GAGTCCCTCC TGACACCCAC CTCTCCCTAC CCGCCCGCGG TGCAGAGCCA GCTTAGCATC 
ACTGCGAACC TCAACCTCTC TAACTCCATG ACCTCACTGG CAACTTCCCA GCATTCCCCA 
GGCATCGACA AGGAGAATGT GGAGCTCTCG CCAACCACCG GGCACTGCAG CGGCGGGAGG 
ACGCGCCACG GCTCCGCCAG CCAGGTCCAG AAGCAAAGGA GTGCCGGCAG CTTCAAGAGG 
CACAGCATCA AGAAGATCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NF1 ( XM_031967657.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031823517.1 Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X18, mRNA.

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CloneID	OSa234705
Clone ID Related Accession (Same CDS sequence)	XM_031967657.1
Accession Version	XM_031967657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	neurofibromin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGCAGCCA AAGGACACGA TGAGGTTAGA TGAAACAATG 
CTGGTCAAAC AGTTGCTGCC GGAAATCTGC CATTTCCTTC ACACCTACCG GGAAGGGAAC 
CAGCACGCAG CCGAGCTTCG GAGCTCCGCC TCTGGCGTCC TTTTCTCCCT CAGCTGTAAT 
AACTTCAATG CCGTCTTCAG CCGGATTTCC ACCAGGTTAC AGGAATTGAC TGTTTGCTCA 
GAAGATAATG TTGATGTCCA TGATATAGAA TTATTACAAT ATATCAATGT GGATTGTGCA 
AAACTAAAGC GGCTCTTGCA GGAAACTGTA TTTAAGTTTA AAGCCTTAAA GAAGGTAGCT 
CAACTAGCAG TTATAAATAG CCTAGAAAAG GCATTTTGGA ATTGGGTAGA GAACTATCCT 
GATGAATTTA CAAAGCTGTA CCAGACTCCC CAGACAGATA TGGCGGAATG TGCAGAAAAA 
TTATTTGACT TGGTGGATGG TTTTGCTGAA AGCACCAAAC GTAAAGCTGC AGTTTGGCCA 
CTGCAAATCA TTCTTCTTGT CTTGTGTCCA GAAATAATCC AGGACATTTC CAAGGATGTG 
GTTGATGAAG CGAAAATGAA TAAGAAAATG TTTCTCGACA ATCTCAGAAG AGCGCTGGCC 
GGCCACAGTG GGAGCAGGCA GCTGACAGAG AGTGCCGCGA TCGCTTGTGT TAAACTGTGC 
AAAGCCAGCA CCTACATCAA CTGGGAGGAT AACTCGGTCA TTTTCCTGCT CGTCCAATCC 
ATGGTGGTGG ATCTGAAGAA CTTGCTCTTT AATCCCAGTA AGCCCTTCTC CAGAGGCAGC 
CAGAATGCTG ATGTGGACCT CATGATAGAC TGCCTGGTTT CTTGCTTTCG CATCAGCCCT 
CACAACAACC AACACTTTAA GATCTGTTTG GCCCAGAACT CCCCATCTAC ATTTCATTAT 
GTGCTGGTAA ACTCCCTCCA TCGAATTATC ACCAATTCCG CCCTGGATTG GTGGCCCAAG 
ATTGATGCTG TGTATTGCCA TTCTGTAGAA CTTCGAAACA TGTTTGGTGA AACGCTTCAC 
AAGGCCGTGC AGGGCTGTGG GCCTCACCCC GCGATACGAA TGGCGCCGAG TCTTACATTT 
AAAGAAAAAA TGACAAGCCT TAAATTTAAA GAAAAACCTA CGGACTTGGA AACAAGAAGT 
TATAAATACC TCTTATTATC CATGGTGAAA CTAATCCATG CAGATCCAAA GCTGTTGCTA 
TGTAATCCAA GAAAACAAGG GCCTGAAACT CAGGGGAGTA CAGCAGAGTT AATCACAGGC 
CTTGTGCAGC TCGTCCCACA GTCCAGCATG CCAGAAATAG CTCAAGAAGC AATGGAGGCT 
TTGCTGGTTC TTCATCAGTT GGATAGTATT GACTTATGGA ATCCAGATTC ACCTATAGAA 
ACATTCTGGG AGATTAGCTC ACAAATGCTT TTTTATATCT GTAAAAAGCT AACTACCCAT 
CAGATGCTTA GCAGTACAGA AATTCTCAAG TGGTTGCGAG AAATTTTGAT CTGTAGGAAC 
AAATATCTTC TTAAAAATAA GCAGTCAGAT AGGAGTTCTT CCCATTTCCT CTACCTTTAT 
GGGGCAGGCT ATGATGTCTC TTCTGGTGGA GGTACCAGTC ATATCTCTCT AGACCACGAG 
GAGTTTATGC GTACCTGTGC TCATGGACCA TCTCTCAGGA AAGTGAAAGG AAGCTCCTCA 
ATGGACGGTG CAATCGGGTC CAGCGGGACG CCCATCTGCC GCCAGGCCCA GACTAAGCTG 
GAGGTTGCTC TGTACATGTT TCTGTGGAGT CCCGACATTG AAGCCGTGCT CGTGGCCATG 
TCCTGCTTTC GACACCTGTG TGAGGAAGCC GACATCAGGT GTGGCATAGA TGAGATCTCG 
GTGCACCATT TCTTGCCAAA CTATAACACC TTCATGGAGT TCGCATCTGT CAGCAACATG 
ATGTCGACAG GAAGAGCAGC TCTTCAGAAA AGAGTGATGG CCCTGCTCAG GCGCATCGAA 
CACCCCACCG CTGGAAACAC CGAGGCTTGG GAAGATACTT ACGCCAAATG GGAGCAAGCT 
ACAAAATCAA TCCTCAATTA TCCGAAAGCC AAAATTGAAG ATGGCCAGGT TGCTGAGAGT 
CTTCACAAGA CCATTGTGAA GAGAAGAATG TCTCACGTGA GTGGAGGGGG CTCCATAGAC 
TTGTGCGACA CTGACTCGCT ACAGGAGTGG ATCAACATGA CTGGGTTTCT CTGTGCCCTT 
GGTGGCGTGT GCCTACAGCA GCGGAGCAAT GCCGGCTTTG CCACCTACAG CCCTCCCATG 
GGCCCTATCA GTGAACGCAA GGGCTCTCTC ATTTCCATGG TGTCCAGCGA TGGAAACGCA 
GAGACGCCTG TCAGCAAGTT CATGGATCGG CTGCTCTCCT TGATGGTGTG TAACCACGAA 
AAAGTGGGGC TGCAGATACG AAGCAATGTC AAGGACTTGT TGGGTCTTGA ATTGAGTCCT 
GCCCTTTATC CAATGCTGTT TAACAAATTA AAAAATACCA TCAGCAAGTT TTTTGATCCT 
CAAGGACAGG TTTTATTGAC TGACACCAAC ACCCAGTTTG TGGAGCAGAC CATAGCCATA 
ATGAAGAACT TGCTTGATAA TCATGCTGAG GGTAGTTCTG AACACATGGG ACAAGCTAGC 
ATCGAAACTA TGATGTTAAA TCTGGTCAGA TATGTTAGAG TGTTTGGGAA TTTGGTGCAG 
GCAATTCAGA TCAAAACTAA GCTGTGCCAG CTGGTTCAAG TAATGATGGA GAGGAGAGAT 
GACCTTTCAT TTTGCCAAGA GATGAAATTT AGGAATAAAA TGGTAGAATA TCTGACAGAT 
TGGGTCATGG GAACATCTAA CCAAGCTGCA GATGAAGATG TGAAATGTCT AACTAGAGAT 
TTGGACCAGG CAAGCATGGA AGCAGTGGTG TCATTGCTCG CTGGGCTCCC TCTGCAGCCT 
GAGGAAGGCG ATGGGGTGGA ATTGATGGAA GCCAAATCCC AGCTGTTCCT TAAGTATTTC 
ACCCTCTTTA TGAACCTTCT TAATGACTGC AGTGAGGTCG AAGAAGAAAA TGTCCAGATT 
GGGGGCAGGA AGCGCGGCAT GTCGCGAAGG CTGGCGTCCC TGCGCCACTG CACCGTGCTG 
GCAATGTCCA ACCTGCTCAA TGCGAATGTA GACAGCGGCC TGATGCACTC TATAGGATTG 
GGTTACCACA AAGATCTTCA GACAAGAGCC ACCTTCATGG AAGTCCTCAC AAAAATCCTT 
CAGCAAGGCA CAGAGTTTGA TACGCTCGCA GAAACCGTGT TAGCTGATCG ATTTGAGAGA 
CTTGTTGAGT TGGTCACGAT GATGGGTGAT CAAGGAGAGC TCCCCATAGC CATGGCTCTG 
GCCAACGTGG TGCCTTGTTC TCAGTGGGAT GAACTTGCTC GAGTCCTTGT TACTCTCTTT 
GATTCCCGAC ACTTACTGTA TCAGCTCCTT TGGAACATGT TTTCTAAGGA AGTTGAGCTG 
GCAGACTCCA TGCAGACACT TTTTAGAGGA AACAGCTTGG CCAGTAAAAT CATGACCTTC 
TGCTTTAAGG TCTACGGTGC TACATACCTG CAGAAGCTTC TGGAGCCTTT ACTCCGCTTT 
GTGATCACAT CCTCCGAATG GCAGCACGTC AGCTTCGAGG TCGATCCCAC CAGGTTAGAG 
CATACTGAGA GCCTCGAGGA CAACCAGCGG AACCTTTTGC AAATGACTGA GAAGTTCTTC 
CACGCCATCA TCAGTTCTTC CTCCGAGTTC CCCCCTCAGC TACGCAGTGT GTGCCACTGC 
CTGTACCAGG TTGTTAGCCA GAGATTCCCT CAGAACAGCA TTGGCGCCGT GGGGAGTGCC 
ATGTTCCTGA GATTCATCAA CCCTGCCGTT GTCTCTCCCT ACGAGGCAGG GATTTTAGAG 
AGAAAACCGC TCCCTAGAAT TGAAAGGGGG TTGAAGCTGA TGTCAAAGAT TCTTCAGAGC 
ATTGCCAATC ATGTCCTTTT CACAAAAGAA GAGTATATGA GGCCTTTTAA CGATTTTGTG 
AAAAGCAACT TTGATGTAGC ACGGAGATTT TTCCTTGATA TCGCATCTGA TTGTCCTGTA 
AGTGATGCCA TAAACCACAG CCTTTCCTTC ATCAGCGATG GCAATGTCTT GGCTTTGCAC 
CGTCTGCTCT GGAACAACCA AGAGAAAATC GGCCAATATC TTTCAAGCAA CAGAGATCAC 
AAAGCTGTTG GAAGGCGACC TTTCGATAAG ATGGCAACAC TGCTTGCCTA CCTGGGACCT 
CCAGAGCACA AGCCTGTGGC TGACACACAC TGGTCCAGCC TAAATCTTAC CAGCTCCAAG 
TTTGAGGAAT TCATGACAAG GCACCAGGTA CATGAAAAAG AGGAATTCAA GGCTCTGAAA 
ACATTAAATA TCTTCTATCA AGCAGGCACT TCCAAAGCTG GAAATCCTGT TTTTTATTAT 
GTCACACGAA GGTTCAAGAC TGGCCAGATC AACGGGGATT TGCTTATATA CCACGTCTTA 
CTAACTTTAA AGCCATATTA TGCAAAGCCA TATGAGATTG TAATGGACCT CACTCATGCC 
GGGCCTAGCA ATCGCTTTAA GACAGACTTC CTCTCCAAGT GGTTTGTTGT TTTCCCGGGC 
TTTGCTTACG AGAACGTCTC TTCGGTCTAC ATCTACAACT GTAATTCTTG GGTTCGGGAG 
TACACCAAAT ACCATGAGAG ACTGCTCACG GGACTCAAAG GCAGCAAAAG ACTCTTTTTC 
ATAGACTCTC CCTCAAAACT GGCTGAGCAT ATCGATTATG ATCAGCAAAA ACTGCCAGCC 
GCGACCTTGG CTCTGGATGA AGACCTCAAG GTGTACAACA GTGCTCTCAA GCTGGCTCAC 
AAGGACACCA AGGTGACCCT CAAGGTTGGC TCTACTGCGA TCCAAGTGAC GTCATCTGAG 
CGAACTAAAG TCCTGGGACA GACGGTTTTT CTGAATGATA TTTACTATGC CTCTGAGATC 
GAAGAAATCT GTCTTGTGGA TGAAAACCAG TTCACTCTGA CCATTTCCAA TCAGGGCACT 
CCCCTCACCT TCATGCATCA AGAGTGTGAA GGCATTGTGG CGTCCATCAT TCACATCCGA 
ACTCGATGGG AGCTGTCCCA GCCAGATTCT ATCCCCCAGC ACACTAAGAT CCGGCCAAAG 
GACGTTCCCG GAACACTTTT GAATATCGCA TTACTTAACT TGGGTAGTTC GGACCCAAGT 
TTACGGTCAG CCGCCTATAA TCTTCTCTGT GCCTTAACCT GTACCTTTAA TTTAAAAATT 
GAGGGCCAAT TACTAGAAAC ATCAGGTCTG TGTATACCTG CCAACAATAC CCTTTTTATT 
GTCTCTATTA GTAAAACCCT GGCAGCTAAT GAGCCACACC TCACCTTAGA GTTTTTGGAA 
GAGTGCATTT CCGGATTTAG CAAATCCAGT ATTGAACTGA AGCACCTGTG TCTAGAATAC 
ATGACTCCCT GGCTATCAAA TCTAGTCCGT TTCTGTAAAC ATAATGATGA TGCCAAACGA 
CAGAGAGTCA CTGCTATTCT TGATAAACTT ATAACAATGA CCATAAATGA AAAGCAGATG 
TACCCATCGA TCCAAGCCAA GATATGGGGA AGCCTTGGGC AGATTACAGA TCTGCTCGAT 
GTAGTGCTGG ACAGCTTTAT CAAAACCAGT GCCACAGGTG GTTTAGGATC CATCAAGGCT 
GAAGTGATGG CTGACACGGC CGTGGCTCTG GCTTCTGGGA ACGTCAAACT AGTTTCGAGC 
AAGGTGATTG GAAGAATGTG CAAAATCATT GACAAGACCT GCCTCTCTCC AACTCCTACC 
TTAGAGCAGC ACCTCATGTG GGATGATATC GCCATTTTAG CACGCTACAT GCTAATGCTG 
TCCTTCAATA ATTCTCTGGA TGTGGCGGCT CATCTTCCCT ACCTCTTCCA TGTGGTGACT 
CTGTTGGTGG CCACAGGTCC CTTGTCCCTT CGTGCTTCCA CACACGGACT GGTCATCAAT 
ATCATCCATT CTCTGTGCAC GTGCTCACAG CTTCACTTTA GTGAAGAGAC CAAGCAAGTT 
TTACGACTGA GTCTGACTGA GTTCTCACTA CCTAAGTTTT ATTTGCTGTT CGGCATCAGC 
AAAGTCAAAT CAGCTGCCGT GATAGCCTTC CGGTCCAGTT ACCGGGACAG GTCTTTCTCC 
CCTGGCTCCT ATGAGAGGGA GACCTTTGCC CTGGCCTCCT TGGAAACAGT CACAGAAGCT 
CTCTTGGAGA TCATGGAGGC TTGTATGAGA GATATTCCTG CATGCAAGTG GCTGGACCAG 
TGGACAGAGC TAGCCCAGAG GTTTGCGTTC CAGTACAATC CCTCCCTGCA GCCACGAGCT 
CTGGTTGTGT TTGGTTGTAT CAGCAAAAGA GTGTCCCATG GGCAGATCAA GCAGATTATC 
CGCATTCTTA GCAAGGGACT TGAGAGCTGC CTAAAAGGAC CTGATAATTA TAACAGTCAA 
GTTCTGATTG AAGCTACCGT GATAGCTCTA ACCAAATTAC AACCACTTCT TAATAAGGAG 
TCCCCTCTGC ACCGAGCTCT CTTCTGGGTG GCCGTTGCCG TGCTGCAGCT GGATGAAGTG 
GACCTGTACT CGGCGGGCAC TGCCCTCCTC GAACAGAACC TGCACACCTT AGACAGCCTC 
CTGCACATCT TCAACGACCA GAGCCCAGAA GAAGTGTTCA TGGAGATCCG GCAGCCTCTG 
GAGTGGCACT GCAAGCAGAT GGACCACTTT GTGGGGCTCA ACTTTAACTC CAGCTTTAAC 
TTTGCCCTGG TCGGGCACCT GCTGAAGGGA TTTCGGCATC CTTCACCTGT CACTGTTGCA 
AGAACTGTGA GGATTTTGCA CACCCTACTA GCATTGGTTA ACAAGCATAG GAATTGTGAC 
AAGTTTGAGG TGAACACCCA GAGTGTGGCT TACTTAGCAG CTTTACTTAC TGTGTCTGAG 
GAGGTTCGAA GTCGCTGCAG CTTAAAGCAT AGGAAATCAC TCCTACTAAC TGATATCACA 
ATGGAAAATG TGCCTATGGA TATATATCCC ATCTATCACA GTGACCCCAG CCTTAGGACA 
CTAAAAGAAA CACAACCGTG GTCCTCTCCA AAGGGATCTG AGAGACATCT TGCAGCCAGC 
TACCCTCCTG TGGGCCAAAC TAGCCCCCGA ACCAGAAAAT CCATGAGCCT GGATATGGGG 
CAGCCTTCCC AGGCCAACAC AAAGAAGTTG CTTGGGACAA GGAAAAGCTT CGATCATTTG 
ATCTCTGATG CCAAAGCTCC CAAAAGGCAA GAACAGGAGT CTGGAGTCAC CACGCCCCCC 
AAGATGCGGA GAGTGGCCGA GAACGACTAC GAGATGGAAA CCCAGAGAAT CTCCCCTCAG 
CCACACCCGC CTCTGCGGAA AGTGTCCGTG TCCGAATCCA ATGTGCTGCT GGACGAGGAA 
GTGCTCACAG ACCCCAAAAC CCAGGCTCTT CTTCTCACTG TCCTGGCTAC TCTGGTGAAG 
TACACTGCGG ACGAGTTTGA CCAGCGCATT CTCTATGAGT ACTTAGCGGA GGCCAGTGTT 
GTCTTCCCAA AGGTTTTTCC AGTGGTACAT AATTTGTTGG ACTCAAAGAT AAATACTCTG 
CTCTCATTAT GCCAAGACCC AAATTTGCTA AACCCGGTTC ATGGAATTGT GCAGAGTGTG 
GTCTACCACG AAGAGGCCCC GCCGCAGTAC CAGACGGCGT ACCTCCAGAG TTTCGGTTTT 
AATGGTCTGT GGAGGTTTGC AGGACCATTT TCTAAGCAGA CGCAGATCCC AGACTATGCC 
GAGCTCATCG TGAAGTTTCT CGATGCCTTG ATTGACACGT ATCTGCCTGG AATTGATGAG 
GAAGCCAGTG AGGAGTCCCT CCTGACACCC ACCTCTCCCT ACCCGCCCGC GGTGCAGAGC 
CAGCTTAGCA TCACTGCGAA CCTCAACCTC TCTAACTCCA TGACCTCACT GGCAACTTCC 
CAGCATTCCC CAGGCATCGA CAAGGAGAAT GTGGAGCTCT CGCCAACCAC CGGGCACTGC 
AGCGGCGGGA GGACGCGCCA CGGCTCCGCC AGCCAGGTCC AGAAGCAAAG GAGTGCCGGC 
AGCTTCAAGA GGCACAGCAT CAAGAAGATC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031823517.1
CDS	9..8444
Translation	MVQAVVNRFDEQLPIKAGQQTTHTKVSTEHNKECLINISKYKFSLVISGLTNILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTYREGNQHAAELRSSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLQETVFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQTPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLVLCPEIIQDISKDVVDEAKMNKKMFLDNLRRALAGHSGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQNADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGPHPAIRMAPSLTFKEKMTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGSTAELITGLVQLVPQSSMPEIAQEAMEALLVLHQLDSIDLWNPDSPIETFWEISSQMLFYICKKLTTHQMLSSTEILKWLREILICRNKYLLKNKQSDRSSSHFLYLYGAGYDVSSGGGTSHISLDHEEFMRTCAHGPSLRKVKGSSSMDGAIGSSGTPICRQAQTKLEVALYMFLWSPDIEAVLVAMSCFRHLCEEADIRCGIDEISVHHFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNTEAWEDTYAKWEQATKSILNYPKAKIEDGQVAESLHKTIVKRRMSHVSGGGSIDLCDTDSLQEWINMTGFLCALGGVCLQQRSNAGFATYSPPMGPISERKGSLISMVSSDGNAETPVSKFMDRLLSLMVCNHEKVGLQIRSNVKDLLGLELSPALYPMLFNKLKNTISKFFDPQGQVLLTDTNTQFVEQTIAIMKNLLDNHAEGSSEHMGQASIETMMLNLVRYVRVFGNLVQAIQIKTKLCQLVQVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADEDVKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEEENVQIGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPLLRFVITSSEWQHVSFEVDPTRLEHTESLEDNQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQVVSQRFPQNSIGAVGSAMFLRFINPAVVSPYEAGILERKPLPRIERGLKLMSKILQSIANHVLFTKEEYMRPFNDFVKSNFDVARRFFLDIASDCPVSDAINHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSKAGNPVFYYVTRRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVMDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVSSVYIYNCNSWVREYTKYHERLLTGLKGSKRLFFIDSPSKLAEHIDYDQQKLPAATLALDEDLKVYNSALKLAHKDTKVTLKVGSTAIQVTSSERTKVLGQTVFLNDIYYASEIEEICLVDENQFTLTISNQGTPLTFMHQECEGIVASIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALASLETVTEALLEIMEACMRDIPACKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKESPLHRALFWVAVAVLQLDEVDLYSAGTALLEQNLHTLDSLLHIFNDQSPEEVFMEIRQPLEWHCKQMDHFVGLNFNSSFNFALVGHLLKGFRHPSPVTVARTVRILHTLLALVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDITMENVPMDIYPIYHSDPSLRTLKETQPWSSPKGSERHLAASYPPVGQTSPRTRKSMSLDMGQPSQANTKKLLGTRKSFDHLISDAKAPKRQEQESGVTTPPKMRRVAENDYEMETQRISPQPHPPLRKVSVSESNVLLDEEVLTDPKTQALLLTVLATLVKYTADEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPVHGIVQSVVYHEEAPPQYQTAYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEEASEESLLTPTSPYPPAVQSQLSITANLNLSNSMTSLATSQHSPGIDKENVELSPTTGHCSGGRTRHGSASQVQKQRSAGSFKRHSIKKIV

Target ORF information:

RefSeq Version	XM_031967657.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii neurofibromin 1 (NF1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031967657.1

ORF Insert Sequence:

ATGGTCCAGG CCGTGGTGAA CCGCTTCGAT GAGCAGCTTC CAATTAAAGC TGGACAGCAG 
ACTACACACA CCAAAGTCAG CACTGAACAC AACAAGGAGT GTCTTATCAA CATTTCCAAA 
TACAAGTTTT CTCTGGTTAT AAGTGGCCTC ACCAATATTT TAAAAAATGT TAACAATATG 
AGAATATTTG GTGAAGCTGC TGAAAAAAAT TTATATCTCT CTCAGTTAAT TATTTTGGAT 
ACACTGGAAA AATGCCTTGC TGGGCAGCCA AAGGACACGA TGAGGTTAGA TGAAACAATG 
CTGGTCAAAC AGTTGCTGCC GGAAATCTGC CATTTCCTTC ACACCTACCG GGAAGGGAAC 
CAGCACGCAG CCGAGCTTCG GAGCTCCGCC TCTGGCGTCC TTTTCTCCCT CAGCTGTAAT 
AACTTCAATG CCGTCTTCAG CCGGATTTCC ACCAGGTTAC AGGAATTGAC TGTTTGCTCA 
GAAGATAATG TTGATGTCCA TGATATAGAA TTATTACAAT ATATCAATGT GGATTGTGCA 
AAACTAAAGC GGCTCTTGCA GGAAACTGTA TTTAAGTTTA AAGCCTTAAA GAAGGTAGCT 
CAACTAGCAG TTATAAATAG CCTAGAAAAG GCATTTTGGA ATTGGGTAGA GAACTATCCT 
GATGAATTTA CAAAGCTGTA CCAGACTCCC CAGACAGATA TGGCGGAATG TGCAGAAAAA 
TTATTTGACT TGGTGGATGG TTTTGCTGAA AGCACCAAAC GTAAAGCTGC AGTTTGGCCA 
CTGCAAATCA TTCTTCTTGT CTTGTGTCCA GAAATAATCC AGGACATTTC CAAGGATGTG 
GTTGATGAAG CGAAAATGAA TAAGAAAATG TTTCTCGACA ATCTCAGAAG AGCGCTGGCC 
GGCCACAGTG GGAGCAGGCA GCTGACAGAG AGTGCCGCGA TCGCTTGTGT TAAACTGTGC 
AAAGCCAGCA CCTACATCAA CTGGGAGGAT AACTCGGTCA TTTTCCTGCT CGTCCAATCC 
ATGGTGGTGG ATCTGAAGAA CTTGCTCTTT AATCCCAGTA AGCCCTTCTC CAGAGGCAGC 
CAGAATGCTG ATGTGGACCT CATGATAGAC TGCCTGGTTT CTTGCTTTCG CATCAGCCCT 
CACAACAACC AACACTTTAA GATCTGTTTG GCCCAGAACT CCCCATCTAC ATTTCATTAT 
GTGCTGGTAA ACTCCCTCCA TCGAATTATC ACCAATTCCG CCCTGGATTG GTGGCCCAAG 
ATTGATGCTG TGTATTGCCA TTCTGTAGAA CTTCGAAACA TGTTTGGTGA AACGCTTCAC 
AAGGCCGTGC AGGGCTGTGG GCCTCACCCC GCGATACGAA TGGCGCCGAG TCTTACATTT 
AAAGAAAAAA TGACAAGCCT TAAATTTAAA GAAAAACCTA CGGACTTGGA AACAAGAAGT 
TATAAATACC TCTTATTATC CATGGTGAAA CTAATCCATG CAGATCCAAA GCTGTTGCTA 
TGTAATCCAA GAAAACAAGG GCCTGAAACT CAGGGGAGTA CAGCAGAGTT AATCACAGGC 
CTTGTGCAGC TCGTCCCACA GTCCAGCATG CCAGAAATAG CTCAAGAAGC AATGGAGGCT 
TTGCTGGTTC TTCATCAGTT GGATAGTATT GACTTATGGA ATCCAGATTC ACCTATAGAA 
ACATTCTGGG AGATTAGCTC ACAAATGCTT TTTTATATCT GTAAAAAGCT AACTACCCAT 
CAGATGCTTA GCAGTACAGA AATTCTCAAG TGGTTGCGAG AAATTTTGAT CTGTAGGAAC 
AAATATCTTC TTAAAAATAA GCAGTCAGAT AGGAGTTCTT CCCATTTCCT CTACCTTTAT 
GGGGCAGGCT ATGATGTCTC TTCTGGTGGA GGTACCAGTC ATATCTCTCT AGACCACGAG 
GAGTTTATGC GTACCTGTGC TCATGGACCA TCTCTCAGGA AAGTGAAAGG AAGCTCCTCA 
ATGGACGGTG CAATCGGGTC CAGCGGGACG CCCATCTGCC GCCAGGCCCA GACTAAGCTG 
GAGGTTGCTC TGTACATGTT TCTGTGGAGT CCCGACATTG AAGCCGTGCT CGTGGCCATG 
TCCTGCTTTC GACACCTGTG TGAGGAAGCC GACATCAGGT GTGGCATAGA TGAGATCTCG 
GTGCACCATT TCTTGCCAAA CTATAACACC TTCATGGAGT TCGCATCTGT CAGCAACATG 
ATGTCGACAG GAAGAGCAGC TCTTCAGAAA AGAGTGATGG CCCTGCTCAG GCGCATCGAA 
CACCCCACCG CTGGAAACAC CGAGGCTTGG GAAGATACTT ACGCCAAATG GGAGCAAGCT 
ACAAAATCAA TCCTCAATTA TCCGAAAGCC AAAATTGAAG ATGGCCAGGT TGCTGAGAGT 
CTTCACAAGA CCATTGTGAA GAGAAGAATG TCTCACGTGA GTGGAGGGGG CTCCATAGAC 
TTGTGCGACA CTGACTCGCT ACAGGAGTGG ATCAACATGA CTGGGTTTCT CTGTGCCCTT 
GGTGGCGTGT GCCTACAGCA GCGGAGCAAT GCCGGCTTTG CCACCTACAG CCCTCCCATG 
GGCCCTATCA GTGAACGCAA GGGCTCTCTC ATTTCCATGG TGTCCAGCGA TGGAAACGCA 
GAGACGCCTG TCAGCAAGTT CATGGATCGG CTGCTCTCCT TGATGGTGTG TAACCACGAA 
AAAGTGGGGC TGCAGATACG AAGCAATGTC AAGGACTTGT TGGGTCTTGA ATTGAGTCCT 
GCCCTTTATC CAATGCTGTT TAACAAATTA AAAAATACCA TCAGCAAGTT TTTTGATCCT 
CAAGGACAGG TTTTATTGAC TGACACCAAC ACCCAGTTTG TGGAGCAGAC CATAGCCATA 
ATGAAGAACT TGCTTGATAA TCATGCTGAG GGTAGTTCTG AACACATGGG ACAAGCTAGC 
ATCGAAACTA TGATGTTAAA TCTGGTCAGA TATGTTAGAG TGTTTGGGAA TTTGGTGCAG 
GCAATTCAGA TCAAAACTAA GCTGTGCCAG CTGGTTCAAG TAATGATGGA GAGGAGAGAT 
GACCTTTCAT TTTGCCAAGA GATGAAATTT AGGAATAAAA TGGTAGAATA TCTGACAGAT 
TGGGTCATGG GAACATCTAA CCAAGCTGCA GATGAAGATG TGAAATGTCT AACTAGAGAT 
TTGGACCAGG CAAGCATGGA AGCAGTGGTG TCATTGCTCG CTGGGCTCCC TCTGCAGCCT 
GAGGAAGGCG ATGGGGTGGA ATTGATGGAA GCCAAATCCC AGCTGTTCCT TAAGTATTTC 
ACCCTCTTTA TGAACCTTCT TAATGACTGC AGTGAGGTCG AAGAAGAAAA TGTCCAGATT 
GGGGGCAGGA AGCGCGGCAT GTCGCGAAGG CTGGCGTCCC TGCGCCACTG CACCGTGCTG 
GCAATGTCCA ACCTGCTCAA TGCGAATGTA GACAGCGGCC TGATGCACTC TATAGGATTG 
GGTTACCACA AAGATCTTCA GACAAGAGCC ACCTTCATGG AAGTCCTCAC AAAAATCCTT 
CAGCAAGGCA CAGAGTTTGA TACGCTCGCA GAAACCGTGT TAGCTGATCG ATTTGAGAGA 
CTTGTTGAGT TGGTCACGAT GATGGGTGAT CAAGGAGAGC TCCCCATAGC CATGGCTCTG 
GCCAACGTGG TGCCTTGTTC TCAGTGGGAT GAACTTGCTC GAGTCCTTGT TACTCTCTTT 
GATTCCCGAC ACTTACTGTA TCAGCTCCTT TGGAACATGT TTTCTAAGGA AGTTGAGCTG 
GCAGACTCCA TGCAGACACT TTTTAGAGGA AACAGCTTGG CCAGTAAAAT CATGACCTTC 
TGCTTTAAGG TCTACGGTGC TACATACCTG CAGAAGCTTC TGGAGCCTTT ACTCCGCTTT 
GTGATCACAT CCTCCGAATG GCAGCACGTC AGCTTCGAGG TCGATCCCAC CAGGTTAGAG 
CATACTGAGA GCCTCGAGGA CAACCAGCGG AACCTTTTGC AAATGACTGA GAAGTTCTTC 
CACGCCATCA TCAGTTCTTC CTCCGAGTTC CCCCCTCAGC TACGCAGTGT GTGCCACTGC 
CTGTACCAGG TTGTTAGCCA GAGATTCCCT CAGAACAGCA TTGGCGCCGT GGGGAGTGCC 
ATGTTCCTGA GATTCATCAA CCCTGCCGTT GTCTCTCCCT ACGAGGCAGG GATTTTAGAG 
AGAAAACCGC TCCCTAGAAT TGAAAGGGGG TTGAAGCTGA TGTCAAAGAT TCTTCAGAGC 
ATTGCCAATC ATGTCCTTTT CACAAAAGAA GAGTATATGA GGCCTTTTAA CGATTTTGTG 
AAAAGCAACT TTGATGTAGC ACGGAGATTT TTCCTTGATA TCGCATCTGA TTGTCCTGTA 
AGTGATGCCA TAAACCACAG CCTTTCCTTC ATCAGCGATG GCAATGTCTT GGCTTTGCAC 
CGTCTGCTCT GGAACAACCA AGAGAAAATC GGCCAATATC TTTCAAGCAA CAGAGATCAC 
AAAGCTGTTG GAAGGCGACC TTTCGATAAG ATGGCAACAC TGCTTGCCTA CCTGGGACCT 
CCAGAGCACA AGCCTGTGGC TGACACACAC TGGTCCAGCC TAAATCTTAC CAGCTCCAAG 
TTTGAGGAAT TCATGACAAG GCACCAGGTA CATGAAAAAG AGGAATTCAA GGCTCTGAAA 
ACATTAAATA TCTTCTATCA AGCAGGCACT TCCAAAGCTG GAAATCCTGT TTTTTATTAT 
GTCACACGAA GGTTCAAGAC TGGCCAGATC AACGGGGATT TGCTTATATA CCACGTCTTA 
CTAACTTTAA AGCCATATTA TGCAAAGCCA TATGAGATTG TAATGGACCT CACTCATGCC 
GGGCCTAGCA ATCGCTTTAA GACAGACTTC CTCTCCAAGT GGTTTGTTGT TTTCCCGGGC 
TTTGCTTACG AGAACGTCTC TTCGGTCTAC ATCTACAACT GTAATTCTTG GGTTCGGGAG 
TACACCAAAT ACCATGAGAG ACTGCTCACG GGACTCAAAG GCAGCAAAAG ACTCTTTTTC 
ATAGACTCTC CCTCAAAACT GGCTGAGCAT ATCGATTATG ATCAGCAAAA ACTGCCAGCC 
GCGACCTTGG CTCTGGATGA AGACCTCAAG GTGTACAACA GTGCTCTCAA GCTGGCTCAC 
AAGGACACCA AGGTGACCCT CAAGGTTGGC TCTACTGCGA TCCAAGTGAC GTCATCTGAG 
CGAACTAAAG TCCTGGGACA GACGGTTTTT CTGAATGATA TTTACTATGC CTCTGAGATC 
GAAGAAATCT GTCTTGTGGA TGAAAACCAG TTCACTCTGA CCATTTCCAA TCAGGGCACT 
CCCCTCACCT TCATGCATCA AGAGTGTGAA GGCATTGTGG CGTCCATCAT TCACATCCGA 
ACTCGATGGG AGCTGTCCCA GCCAGATTCT ATCCCCCAGC ACACTAAGAT CCGGCCAAAG 
GACGTTCCCG GAACACTTTT GAATATCGCA TTACTTAACT TGGGTAGTTC GGACCCAAGT 
TTACGGTCAG CCGCCTATAA TCTTCTCTGT GCCTTAACCT GTACCTTTAA TTTAAAAATT 
GAGGGCCAAT TACTAGAAAC ATCAGGTCTG TGTATACCTG CCAACAATAC CCTTTTTATT 
GTCTCTATTA GTAAAACCCT GGCAGCTAAT GAGCCACACC TCACCTTAGA GTTTTTGGAA 
GAGTGCATTT CCGGATTTAG CAAATCCAGT ATTGAACTGA AGCACCTGTG TCTAGAATAC 
ATGACTCCCT GGCTATCAAA TCTAGTCCGT TTCTGTAAAC ATAATGATGA TGCCAAACGA 
CAGAGAGTCA CTGCTATTCT TGATAAACTT ATAACAATGA CCATAAATGA AAAGCAGATG 
TACCCATCGA TCCAAGCCAA GATATGGGGA AGCCTTGGGC AGATTACAGA TCTGCTCGAT 
GTAGTGCTGG ACAGCTTTAT CAAAACCAGT GCCACAGGTG GTTTAGGATC CATCAAGGCT 
GAAGTGATGG CTGACACGGC CGTGGCTCTG GCTTCTGGGA ACGTCAAACT AGTTTCGAGC 
AAGGTGATTG GAAGAATGTG CAAAATCATT GACAAGACCT GCCTCTCTCC AACTCCTACC 
TTAGAGCAGC ACCTCATGTG GGATGATATC GCCATTTTAG CACGCTACAT GCTAATGCTG 
TCCTTCAATA ATTCTCTGGA TGTGGCGGCT CATCTTCCCT ACCTCTTCCA TGTGGTGACT 
CTGTTGGTGG CCACAGGTCC CTTGTCCCTT CGTGCTTCCA CACACGGACT GGTCATCAAT 
ATCATCCATT CTCTGTGCAC GTGCTCACAG CTTCACTTTA GTGAAGAGAC CAAGCAAGTT 
TTACGACTGA GTCTGACTGA GTTCTCACTA CCTAAGTTTT ATTTGCTGTT CGGCATCAGC 
AAAGTCAAAT CAGCTGCCGT GATAGCCTTC CGGTCCAGTT ACCGGGACAG GTCTTTCTCC 
CCTGGCTCCT ATGAGAGGGA GACCTTTGCC CTGGCCTCCT TGGAAACAGT CACAGAAGCT 
CTCTTGGAGA TCATGGAGGC TTGTATGAGA GATATTCCTG CATGCAAGTG GCTGGACCAG 
TGGACAGAGC TAGCCCAGAG GTTTGCGTTC CAGTACAATC CCTCCCTGCA GCCACGAGCT 
CTGGTTGTGT TTGGTTGTAT CAGCAAAAGA GTGTCCCATG GGCAGATCAA GCAGATTATC 
CGCATTCTTA GCAAGGGACT TGAGAGCTGC CTAAAAGGAC CTGATAATTA TAACAGTCAA 
GTTCTGATTG AAGCTACCGT GATAGCTCTA ACCAAATTAC AACCACTTCT TAATAAGGAG 
TCCCCTCTGC ACCGAGCTCT CTTCTGGGTG GCCGTTGCCG TGCTGCAGCT GGATGAAGTG 
GACCTGTACT CGGCGGGCAC TGCCCTCCTC GAACAGAACC TGCACACCTT AGACAGCCTC 
CTGCACATCT TCAACGACCA GAGCCCAGAA GAAGTGTTCA TGGAGATCCG GCAGCCTCTG 
GAGTGGCACT GCAAGCAGAT GGACCACTTT GTGGGGCTCA ACTTTAACTC CAGCTTTAAC 
TTTGCCCTGG TCGGGCACCT GCTGAAGGGA TTTCGGCATC CTTCACCTGT CACTGTTGCA 
AGAACTGTGA GGATTTTGCA CACCCTACTA GCATTGGTTA ACAAGCATAG GAATTGTGAC 
AAGTTTGAGG TGAACACCCA GAGTGTGGCT TACTTAGCAG CTTTACTTAC TGTGTCTGAG 
GAGGTTCGAA GTCGCTGCAG CTTAAAGCAT AGGAAATCAC TCCTACTAAC TGATATCACA 
ATGGAAAATG TGCCTATGGA TATATATCCC ATCTATCACA GTGACCCCAG CCTTAGGACA 
CTAAAAGAAA CACAACCGTG GTCCTCTCCA AAGGGATCTG AGAGACATCT TGCAGCCAGC 
TACCCTCCTG TGGGCCAAAC TAGCCCCCGA ACCAGAAAAT CCATGAGCCT GGATATGGGG 
CAGCCTTCCC AGGCCAACAC AAAGAAGTTG CTTGGGACAA GGAAAAGCTT CGATCATTTG 
ATCTCTGATG CCAAAGCTCC CAAAAGGCAA GAACAGGAGT CTGGAGTCAC CACGCCCCCC 
AAGATGCGGA GAGTGGCCGA GAACGACTAC GAGATGGAAA CCCAGAGAAT CTCCCCTCAG 
CCACACCCGC CTCTGCGGAA AGTGTCCGTG TCCGAATCCA ATGTGCTGCT GGACGAGGAA 
GTGCTCACAG ACCCCAAAAC CCAGGCTCTT CTTCTCACTG TCCTGGCTAC TCTGGTGAAG 
TACACTGCGG ACGAGTTTGA CCAGCGCATT CTCTATGAGT ACTTAGCGGA GGCCAGTGTT 
GTCTTCCCAA AGGTTTTTCC AGTGGTACAT AATTTGTTGG ACTCAAAGAT AAATACTCTG 
CTCTCATTAT GCCAAGACCC AAATTTGCTA AACCCGGTTC ATGGAATTGT GCAGAGTGTG 
GTCTACCACG AAGAGGCCCC GCCGCAGTAC CAGACGGCGT ACCTCCAGAG TTTCGGTTTT 
AATGGTCTGT GGAGGTTTGC AGGACCATTT TCTAAGCAGA CGCAGATCCC AGACTATGCC 
GAGCTCATCG TGAAGTTTCT CGATGCCTTG ATTGACACGT ATCTGCCTGG AATTGATGAG 
GAAGCCAGTG AGGAGTCCCT CCTGACACCC ACCTCTCCCT ACCCGCCCGC GGTGCAGAGC 
CAGCTTAGCA TCACTGCGAA CCTCAACCTC TCTAACTCCA TGACCTCACT GGCAACTTCC 
CAGCATTCCC CAGGCATCGA CAAGGAGAAT GTGGAGCTCT CGCCAACCAC CGGGCACTGC 
AGCGGCGGGA GGACGCGCCA CGGCTCCGCC AGCCAGGTCC AGAAGCAAAG GAGTGCCGGC 
AGCTTCAAGA GGCACAGCAT CAAGAAGATC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NF1
Entrez Gene ID	100923881
Full Name	neurofibromin 1
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NF1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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