USP47 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP47
Entrez Gene ID	100918754
Full Name	ubiquitin specific peptidase 47
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012552517.1	XP_012407971.1	ubiquitin carboxyl-terminal hydrolase 47 isoform X1
XM_012552517.2	XP_012407971.1	ubiquitin carboxyl-terminal hydrolase 47 isoform X1
XM_031944166.1	XP_031800026.1	ubiquitin carboxyl-terminal hydrolase 47 isoform X3
XM_031944165.1	XP_031800025.1	ubiquitin carboxyl-terminal hydrolase 47 isoform X2
XM_012552518.1	XP_012407972.1	ubiquitin carboxyl-terminal hydrolase 47 isoform X2
XM_012552518.2	XP_012407972.1	ubiquitin carboxyl-terminal hydrolase 47 isoform X2
XM_023506281.1	XP_023362049.1	ubiquitin carboxyl-terminal hydrolase 47 isoform X3
XM_031944164.1	XP_031800024.1	ubiquitin carboxyl-terminal hydrolase 47 isoform X1

Pathways from BioSystems
Gene Ontology

The following USP47 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP47 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa135893	XM_012552517.2 Latest version!	Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa135893	XM_031944165.1 Latest version!	Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa135894	XM_012552518.2 Latest version!	Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa157461	XM_031944164.1 Latest version!	Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa157461	XM_023506281.1 Latest version!	Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa240696	XM_031944166.1 Latest version!	Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OSa135893	XM_012552517.1	Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa135894	XM_012552518.1	Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP47 ( XM_012552517.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012407971.1 Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa135893
Clone ID Related Accession (Same CDS sequence)	XM_012552517.1 , XM_012552517.2 , XM_031944165.1
Accession Version	XM_012552517.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003846892.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCGG GCGAAGAGAA CCAGCTAGTC CCGAAGGAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTTT TATGTATTAT ACAAGATACT ACTAATTCAA AGACAGTCAA TGAACGAATC 
ACTTTAAATT TACCAGCTTC TACTCCACTG AGGAAACTAT TTGAAGATGT TGCCAACAAA 
GTGGGCTATG TAAATGGAAC CTTTGATTTG GTATGGGGAA ATGGAATCAA TGCTACTGAC 
ATGCCTCCTT TGGATCACAC CAGTGAAAAA TCTCTTCTTG ACACTAACTT TGAACCTGGG 
AAGAAAAACT TTCTGCATTT GACAGATAAA GATGGTGAAC AGCCTCAGAT AGCAGAGGAT 
ACCATTGCAG CAGATGACAG TGTGCAAGAT AGGTTTATAG GCCCTCTTCC AAGAGAAGGT 
TCTGTAGGCT CTACCAATGA TTATGTCGGT CAAAGCTATT CATATTCTTC AATCTTGAAT 
AAATCAGAAA CAGGATATGT GGGATTAGTA AATCAAGCAA TGACTTGCTA TTTGAACAGC 
CTTTTGCAAA CACTTTTCAT GACTCCTGAA TTCAGGAATG CACTGTATAA GTGGGAATTT 
GAGGAATCTG AAGAAGATCC TGTGACAAGC ATTCCATACC AGCTTCAGAG ACTCTTTGTT 
TTATTACAAA CCAGCAAAAA GAGAGCAATT GAAACGACTG ATGTTACAAG AAGCTTTGGA 
TGGGATAGTA GTGAGGCTTG GCAACAACAT GATGTACAAG AACTTTGTAG AGTCATGTTT 
GATGCATTGG AACAGAAATG GAAGCAAACA GAGCAGGCCG ATCTTATAAA TGAACTGTAT 
CAAGGAAAGC TGAAGGACTA CGTGAGATGT CTGGAATGTG GTTATGAGGG CTGGAGAATC 
GACACATATC TTGACATTCC ATTGGTCATC CGACCTTATG GTGCCAGCCA GGCATTTGCT 
AGTGTGGAAG AAGCATTACA AGCATTTATA CAGCCAGAAA TCCTGGATGG CCCCAACCAG 
TACTTTTGTG AGCAGTGTAA AAAGAAGTGT GATGCACGGA AGGGCCTTCG TTTTTTGCAT 
TTCCCATATC TGCTGACTCT CCAGTTGAAA AGGTTTGACT TTGATTACAC CACCATGCAC 
AGGATTAAAT TAAATGACCG GATGACTTTC CCAGAAGAGC TAGATATGAG TACATTTATT 
GATGTTGAAG ATGAGAAGTC TCCTCAGACT GAGAGTTGTA CCGACAGTGG AGCTGAAAAT 
GAAGGCAGTT GTCACAGTGA TCAGATGAGC AACGATTTCT CAAATGATGA TGGGGTCGAT 
GAAGGAATCT GCCTTGAAAG TAATAGTGGA ACTGAAAAGA TCACAAAACC TGGTCTTGAA 
AAGAATTCCT TGCTATACGA ACTCTTTTCT GTCATGGTTC ATTCAGGAAG TGCAGCTGGT 
GGTCATTATT ATGCGTGCAT AAAATCATTC AGTGATGAGC AGTGGTACAG CTTCAATGAT 
CAGCATGTTA GTCGGATAAC CCAAGAGGAC ATTAAGAAGA CTCATGGCGG ATCTTCAGGA 
AGTCGAGGAT ATTATTCTAG TGCCTTTGCC AGCTCCACAA ATGCATATAT GCTTATATAT 
AGATTGAAGG ATCCTTCAAG AAATGCAAAA TTTTTAGAAG TGAAAGATTA CCCAGAGCAC 
ATTAAGAATT TGGTGCGAAA AGAGAAAGAA CTGGAAGAGC AAGAAAAAAA GCAACGTGAA 
ATTGAGCGAA ATACCTGCAA GATAAAATTG TTCTGCATGC ATCCTGTGAA ACAAGTGATG 
ATGGAAAATA AATTGGAGGT TCATAAAGAT AAGACCTTAA AAGAAGCAGT AGAAATAGCT 
TATAAGGTGA TGGATTTAGA AGAAGCCATT CCCCTGGACT GCTGTCGTCT TGTTAAGTAT 
GATGAGTTTC ATGATTATCT AGAGAGGTCA TATGAAGGAG AAGAGGATAC TCCAATGGGA 
CTTCTACTTG GTGGAGTCAA ATCAACATAC ATGTTTGATC TGCTCTTGGA AATCAAAAAA 
CCTGATCAGG TTTTTCAGTC TTATAAACCT GGTGAGGTGA TGGTGAAGGT CCATGTGGTT 
GATATAAAGG CAGAGTCTGT TGCTCCTCCA TTTAGTGTAC GAGCTTATTT AAATCAAACA 
GTCGCCGAGT TTAAGCAACT TATTTCAAAG ACTATACAGC TGCCTGCTGA AACGATGAGA 
ATAGTTCTAG AACGCTGCTA CAATGATTTG CGTCTTCTCA ATGTGCCCAG TAAAACACTG 
AAGGCTGAAG GCTTCTTTAG AAGTAACAAG GTATTTGTCG AAAGTTCTGA GTCTTTGGAT 
CACCAGATGG CCTTTACAGA TTCTCATTTG TGGAAATTGC TGGATCGACA TGCAAATACA 
ATCAGACTGT TTGTTTTATT ACCTGAACAA TCTCCAGGAT CTCATTCTAA AAGGACAGTG 
TACCAGAAAG CTGGTGGGGA TTCTGGCAAC ATGGATGATG CTTGTGAACG AGTTAAAGGA 
CCTCTAGGTA GCCTAAAATC TGTAGAGGCT ATCTTAGAAG AAAGTACTGA AAAGCTTAAA 
AGCTTGTCTC TTCGACAGCA ACAGGATGGA GATAATGGAG ACAGTAGCAA GAGTACAGAG 
ACAAGTGATT TTGAAAACAT TGAATCACCT CTAAATGAGA GAGACTCTTC AGGATCAGTA 
GATACCAGAG AACTTGAACA GCAGATTCCC ACTTCTGATC CAGAAAATTT CCAGTCTGAA 
GAGCGATCCG ACTCTGATGT GAACAATGAC AGGAGTACAA GCTCAGTGGA CAGTGACATT 
CTTAGTTCCA GTCACAGTAG TGACACTTTG TGCAATGCAG ACAGTGCCCC GATCCCTTTG 
GCTAATGGAC TTGACTCTCA TAGTATCACA AGTAGTAGAA GATCTAAGGC AAATGAAGGA 
AAAAAGGAGA CCTGGGATAC AGCTGAAGAA GACTCTGGAA CTGACAGTGA ATATGATGAA 
AGTGGCAAGA GTAGGGGAGA GACACAGTAC ATATATTTCA AAGCAGAATC CTATCCTGCA 
GATGAAGGTT CTGGGGAAGG ACATAAATGG TTGATGGTGC ATGTTGATAA AAGAATTACA 
CTGGCTACCT TCAAACAACA GTTGGAACCC TTTGTTGGAG CTCTGTCTTC TCACTTCAAA 
GTTTTTCGAG TATATGCCAG CAATCAAGAG TTTGAAAGCA TCCGACTGAA TGAAACACTT 
TCATCATTTT CAGATGACAA CAAGATTACA ATTAGATTGG GGAGAGCACT TAAGAAGGGA 
GAGTACAGAG TCAAAGTGTA CCAGCTTTTA GTAAATGAGC AAGAGCCCTG TAGGTTTCTG 
CTTGATGCGG TTTTTGCTAA AGGCATGACT GTTCGACAGT CTAAAGAAGA GCTCATCCCC 
CAGCTCAGGG AGCAGTGTGG GCTAGAAGTC AGCATTGACA GGTTTCGTCT AAGGAAAAAA 
ACATGGAAGA ATCCTGGCAC AGTCTTTTTG GATTATCATG TCTATGAAGA GGATATTAAT 
ATTTCCAGCA ACTGGGAGGT TTTCCTTGAA GTTCTTGATG AAGTGGAGAG GATGAAATCC 
ATGTCACAAT TGGCTGTACT GGCCAGAAGA TGGAGACCAA CAGAGCTGAT ACTGGACCCC 
TTCCAAGAGA TAGTGTTGGA AAGCAGTAGT GTTGATGAAC TGAGAGAGAA GCTCTGCGAA 
ATCAGTGGGA TACCTTTGGA AGATGTGGAA TTTGCAAAGG GAAGAGGAAC ATTTCCCTGT 
GACATGTCTG TCCTAGAAAT TCACCAGGAT TTGGATTGGA ATCCCAAAGT TTCTACATTG 
AATGTGTGGC CCCTTTATAT CTGTGATGAT GGTGCGGTGA TATTTTATAG GGATAAAACT 
GAAGAATTAT TGGAATTAAC AGATGAGCAG AGAAATGAAC TAATGAAAAA GGAAAGCAGC 
CGGCTCCAGA AAACTGGACA TCGTGTAACC TACTCTCCTC GTAAAGAAAA GGCACTAAAA 
ATTTATCTGG ACGGATCATC AAATAAAGAT TTGACTCAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012407971.1
CDS	311..4375
Translation	MVPGEENQLVPKEIENAAEEPRVLCIIQDTTNSKTVNERITLNLPASTPLRKLFEDVANKVGYVNGTFDLVWGNGINATDMPPLDHTSEKSLLDTNFEPGKKNFLHLTDKDGEQPQIAEDTIAADDSVQDRFIGPLPREGSVGSTNDYVGQSYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEESEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGASQAFASVEEALQAFIQPEILDGPNQYFCEQCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMSTFIDVEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSNDDGVDEGICLESNSGTEKITKPGLEKNSLLYELFSVMVHSGSAAGGHYYACIKSFSDEQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPSRNAKFLEVKDYPEHIKNLVRKEKELEEQEKKQREIERNTCKIKLFCMHPVKQVMMENKLEVHKDKTLKEAVEIAYKVMDLEEAIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLEIKKPDQVFQSYKPGEVMVKVHVVDIKAESVAPPFSVRAYLNQTVAEFKQLISKTIQLPAETMRIVLERCYNDLRLLNVPSKTLKAEGFFRSNKVFVESSESLDHQMAFTDSHLWKLLDRHANTIRLFVLLPEQSPGSHSKRTVYQKAGGDSGNMDDACERVKGPLGSLKSVEAILEESTEKLKSLSLRQQQDGDNGDSSKSTETSDFENIESPLNERDSSGSVDTRELEQQIPTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSAPIPLANGLDSHSITSSRRSKANEGKKETWDTAEEDSGTDSEYDESGKSRGETQYIYFKAESYPADEGSGEGHKWLMVHVDKRITLATFKQQLEPFVGALSSHFKVFRVYASNQEFESIRLNETLSSFSDDNKITIRLGRALKKGEYRVKVYQLLVNEQEPCRFLLDAVFAKGMTVRQSKEELIPQLREQCGLEVSIDRFRLRKKTWKNPGTVFLDYHVYEEDINISSNWEVFLEVLDEVERMKSMSQLAVLARRWRPTELILDPFQEIVLESSSVDELREKLCEISGIPLEDVEFAKGRGTFPCDMSVLEIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDKTEELLELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGSSNKDLTQD

Target ORF information:

RefSeq Version	XM_012552517.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012552517.1

ORF Insert Sequence:

ATGGTCCCGG GCGAAGAGAA CCAGCTAGTC CCGAAGGAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTTT TATGTATTAT ACAAGATACT ACTAATTCAA AGACAGTCAA TGAACGAATC 
ACTTTAAATT TACCAGCTTC TACTCCACTG AGGAAACTAT TTGAAGATGT TGCCAACAAA 
GTGGGCTATG TAAATGGAAC CTTTGATTTG GTATGGGGAA ATGGAATCAA TGCTACTGAC 
ATGCCTCCTT TGGATCACAC CAGTGAAAAA TCTCTTCTTG ACACTAACTT TGAACCTGGG 
AAGAAAAACT TTCTGCATTT GACAGATAAA GATGGTGAAC AGCCTCAGAT AGCAGAGGAT 
ACCATTGCAG CAGATGACAG TGTGCAAGAT AGGTTTATAG GCCCTCTTCC AAGAGAAGGT 
TCTGTAGGCT CTACCAATGA TTATGTCGGT CAAAGCTATT CATATTCTTC AATCTTGAAT 
AAATCAGAAA CAGGATATGT GGGATTAGTA AATCAAGCAA TGACTTGCTA TTTGAACAGC 
CTTTTGCAAA CACTTTTCAT GACTCCTGAA TTCAGGAATG CACTGTATAA GTGGGAATTT 
GAGGAATCTG AAGAAGATCC TGTGACAAGC ATTCCATACC AGCTTCAGAG ACTCTTTGTT 
TTATTACAAA CCAGCAAAAA GAGAGCAATT GAAACGACTG ATGTTACAAG AAGCTTTGGA 
TGGGATAGTA GTGAGGCTTG GCAACAACAT GATGTACAAG AACTTTGTAG AGTCATGTTT 
GATGCATTGG AACAGAAATG GAAGCAAACA GAGCAGGCCG ATCTTATAAA TGAACTGTAT 
CAAGGAAAGC TGAAGGACTA CGTGAGATGT CTGGAATGTG GTTATGAGGG CTGGAGAATC 
GACACATATC TTGACATTCC ATTGGTCATC CGACCTTATG GTGCCAGCCA GGCATTTGCT 
AGTGTGGAAG AAGCATTACA AGCATTTATA CAGCCAGAAA TCCTGGATGG CCCCAACCAG 
TACTTTTGTG AGCAGTGTAA AAAGAAGTGT GATGCACGGA AGGGCCTTCG TTTTTTGCAT 
TTCCCATATC TGCTGACTCT CCAGTTGAAA AGGTTTGACT TTGATTACAC CACCATGCAC 
AGGATTAAAT TAAATGACCG GATGACTTTC CCAGAAGAGC TAGATATGAG TACATTTATT 
GATGTTGAAG ATGAGAAGTC TCCTCAGACT GAGAGTTGTA CCGACAGTGG AGCTGAAAAT 
GAAGGCAGTT GTCACAGTGA TCAGATGAGC AACGATTTCT CAAATGATGA TGGGGTCGAT 
GAAGGAATCT GCCTTGAAAG TAATAGTGGA ACTGAAAAGA TCACAAAACC TGGTCTTGAA 
AAGAATTCCT TGCTATACGA ACTCTTTTCT GTCATGGTTC ATTCAGGAAG TGCAGCTGGT 
GGTCATTATT ATGCGTGCAT AAAATCATTC AGTGATGAGC AGTGGTACAG CTTCAATGAT 
CAGCATGTTA GTCGGATAAC CCAAGAGGAC ATTAAGAAGA CTCATGGCGG ATCTTCAGGA 
AGTCGAGGAT ATTATTCTAG TGCCTTTGCC AGCTCCACAA ATGCATATAT GCTTATATAT 
AGATTGAAGG ATCCTTCAAG AAATGCAAAA TTTTTAGAAG TGAAAGATTA CCCAGAGCAC 
ATTAAGAATT TGGTGCGAAA AGAGAAAGAA CTGGAAGAGC AAGAAAAAAA GCAACGTGAA 
ATTGAGCGAA ATACCTGCAA GATAAAATTG TTCTGCATGC ATCCTGTGAA ACAAGTGATG 
ATGGAAAATA AATTGGAGGT TCATAAAGAT AAGACCTTAA AAGAAGCAGT AGAAATAGCT 
TATAAGGTGA TGGATTTAGA AGAAGCCATT CCCCTGGACT GCTGTCGTCT TGTTAAGTAT 
GATGAGTTTC ATGATTATCT AGAGAGGTCA TATGAAGGAG AAGAGGATAC TCCAATGGGA 
CTTCTACTTG GTGGAGTCAA ATCAACATAC ATGTTTGATC TGCTCTTGGA AATCAAAAAA 
CCTGATCAGG TTTTTCAGTC TTATAAACCT GGTGAGGTGA TGGTGAAGGT CCATGTGGTT 
GATATAAAGG CAGAGTCTGT TGCTCCTCCA TTTAGTGTAC GAGCTTATTT AAATCAAACA 
GTCGCCGAGT TTAAGCAACT TATTTCAAAG ACTATACAGC TGCCTGCTGA AACGATGAGA 
ATAGTTCTAG AACGCTGCTA CAATGATTTG CGTCTTCTCA ATGTGCCCAG TAAAACACTG 
AAGGCTGAAG GCTTCTTTAG AAGTAACAAG GTATTTGTCG AAAGTTCTGA GTCTTTGGAT 
CACCAGATGG CCTTTACAGA TTCTCATTTG TGGAAATTGC TGGATCGACA TGCAAATACA 
ATCAGACTGT TTGTTTTATT ACCTGAACAA TCTCCAGGAT CTCATTCTAA AAGGACAGTG 
TACCAGAAAG CTGGTGGGGA TTCTGGCAAC ATGGATGATG CTTGTGAACG AGTTAAAGGA 
CCTCTAGGTA GCCTAAAATC TGTAGAGGCT ATCTTAGAAG AAAGTACTGA AAAGCTTAAA 
AGCTTGTCTC TTCGACAGCA ACAGGATGGA GATAATGGAG ACAGTAGCAA GAGTACAGAG 
ACAAGTGATT TTGAAAACAT TGAATCACCT CTAAATGAGA GAGACTCTTC AGGATCAGTA 
GATACCAGAG AACTTGAACA GCAGATTCCC ACTTCTGATC CAGAAAATTT CCAGTCTGAA 
GAGCGATCCG ACTCTGATGT GAACAATGAC AGGAGTACAA GCTCAGTGGA CAGTGACATT 
CTTAGTTCCA GTCACAGTAG TGACACTTTG TGCAATGCAG ACAGTGCCCC GATCCCTTTG 
GCTAATGGAC TTGACTCTCA TAGTATCACA AGTAGTAGAA GATCTAAGGC AAATGAAGGA 
AAAAAGGAGA CCTGGGATAC AGCTGAAGAA GACTCTGGAA CTGACAGTGA ATATGATGAA 
AGTGGCAAGA GTAGGGGAGA GACACAGTAC ATATATTTCA AAGCAGAATC CTATCCTGCA 
GATGAAGGTT CTGGGGAAGG ACATAAATGG TTGATGGTGC ATGTTGATAA AAGAATTACA 
CTGGCTACCT TCAAACAACA GTTGGAACCC TTTGTTGGAG CTCTGTCTTC TCACTTCAAA 
GTTTTTCGAG TATATGCCAG CAATCAAGAG TTTGAAAGCA TCCGACTGAA TGAAACACTT 
TCATCATTTT CAGATGACAA CAAGATTACA ATTAGATTGG GGAGAGCACT TAAGAAGGGA 
GAGTACAGAG TCAAAGTGTA CCAGCTTTTA GTAAATGAGC AAGAGCCCTG TAGGTTTCTG 
CTTGATGCGG TTTTTGCTAA AGGCATGACT GTTCGACAGT CTAAAGAAGA GCTCATCCCC 
CAGCTCAGGG AGCAGTGTGG GCTAGAAGTC AGCATTGACA GGTTTCGTCT AAGGAAAAAA 
ACATGGAAGA ATCCTGGCAC AGTCTTTTTG GATTATCATG TCTATGAAGA GGATATTAAT 
ATTTCCAGCA ACTGGGAGGT TTTCCTTGAA GTTCTTGATG AAGTGGAGAG GATGAAATCC 
ATGTCACAAT TGGCTGTACT GGCCAGAAGA TGGAGACCAA CAGAGCTGAT ACTGGACCCC 
TTCCAAGAGA TAGTGTTGGA AAGCAGTAGT GTTGATGAAC TGAGAGAGAA GCTCTGCGAA 
ATCAGTGGGA TACCTTTGGA AGATGTGGAA TTTGCAAAGG GAAGAGGAAC ATTTCCCTGT 
GACATGTCTG TCCTAGAAAT TCACCAGGAT TTGGATTGGA ATCCCAAAGT TTCTACATTG 
AATGTGTGGC CCCTTTATAT CTGTGATGAT GGTGCGGTGA TATTTTATAG GGATAAAACT 
GAAGAATTAT TGGAATTAAC AGATGAGCAG AGAAATGAAC TAATGAAAAA GGAAAGCAGC 
CGGCTCCAGA AAACTGGACA TCGTGTAACC TACTCTCCTC GTAAAGAAAA GGCACTAAAA 
ATTTATCTGG ACGGATCATC AAATAAAGAT TTGACTCAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP47 ( XM_012552517.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012407971.1 Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa135893
Clone ID Related Accession (Same CDS sequence)	XM_012552517.1 , XM_012552517.2 , XM_031944165.1
Accession Version	XM_012552517.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003846892.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012552517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCGG GCGAAGAGAA CCAGCTAGTC CCGAAGGAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTTT TATGTATTAT ACAAGATACT ACTAATTCAA AGACAGTCAA TGAACGAATC 
ACTTTAAATT TACCAGCTTC TACTCCACTG AGGAAACTAT TTGAAGATGT TGCCAACAAA 
GTGGGCTATG TAAATGGAAC CTTTGATTTG GTATGGGGAA ATGGAATCAA TGCTACTGAC 
ATGCCTCCTT TGGATCACAC CAGTGAAAAA TCTCTTCTTG ACACTAACTT TGAACCTGGG 
AAGAAAAACT TTCTGCATTT GACAGATAAA GATGGTGAAC AGCCTCAGAT AGCAGAGGAT 
ACCATTGCAG CAGATGACAG TGTGCAAGAT AGGTTTATAG GCCCTCTTCC AAGAGAAGGT 
TCTGTAGGCT CTACCAATGA TTATGTCGGT CAAAGCTATT CATATTCTTC AATCTTGAAT 
AAATCAGAAA CAGGATATGT GGGATTAGTA AATCAAGCAA TGACTTGCTA TTTGAACAGC 
CTTTTGCAAA CACTTTTCAT GACTCCTGAA TTCAGGAATG CACTGTATAA GTGGGAATTT 
GAGGAATCTG AAGAAGATCC TGTGACAAGC ATTCCATACC AGCTTCAGAG ACTCTTTGTT 
TTATTACAAA CCAGCAAAAA GAGAGCAATT GAAACGACTG ATGTTACAAG AAGCTTTGGA 
TGGGATAGTA GTGAGGCTTG GCAACAACAT GATGTACAAG AACTTTGTAG AGTCATGTTT 
GATGCATTGG AACAGAAATG GAAGCAAACA GAGCAGGCCG ATCTTATAAA TGAACTGTAT 
CAAGGAAAGC TGAAGGACTA CGTGAGATGT CTGGAATGTG GTTATGAGGG CTGGAGAATC 
GACACATATC TTGACATTCC ATTGGTCATC CGACCTTATG GTGCCAGCCA GGCATTTGCT 
AGTGTGGAAG AAGCATTACA AGCATTTATA CAGCCAGAAA TCCTGGATGG CCCCAACCAG 
TACTTTTGTG AGCAGTGTAA AAAGAAGTGT GATGCACGGA AGGGCCTTCG TTTTTTGCAT 
TTCCCATATC TGCTGACTCT CCAGTTGAAA AGGTTTGACT TTGATTACAC CACCATGCAC 
AGGATTAAAT TAAATGACCG GATGACTTTC CCAGAAGAGC TAGATATGAG TACATTTATT 
GATGTTGAAG ATGAGAAGTC TCCTCAGACT GAGAGTTGTA CCGACAGTGG AGCTGAAAAT 
GAAGGCAGTT GTCACAGTGA TCAGATGAGC AACGATTTCT CAAATGATGA TGGGGTCGAT 
GAAGGAATCT GCCTTGAAAG TAATAGTGGA ACTGAAAAGA TCACAAAACC TGGTCTTGAA 
AAGAATTCCT TGCTATACGA ACTCTTTTCT GTCATGGTTC ATTCAGGAAG TGCAGCTGGT 
GGTCATTATT ATGCGTGCAT AAAATCATTC AGTGATGAGC AGTGGTACAG CTTCAATGAT 
CAGCATGTTA GTCGGATAAC CCAAGAGGAC ATTAAGAAGA CTCATGGCGG ATCTTCAGGA 
AGTCGAGGAT ATTATTCTAG TGCCTTTGCC AGCTCCACAA ATGCATATAT GCTTATATAT 
AGATTGAAGG ATCCTTCAAG AAATGCAAAA TTTTTAGAAG TGAAAGATTA CCCAGAGCAC 
ATTAAGAATT TGGTGCGAAA AGAGAAAGAA CTGGAAGAGC AAGAAAAAAA GCAACGTGAA 
ATTGAGCGAA ATACCTGCAA GATAAAATTG TTCTGCATGC ATCCTGTGAA ACAAGTGATG 
ATGGAAAATA AATTGGAGGT TCATAAAGAT AAGACCTTAA AAGAAGCAGT AGAAATAGCT 
TATAAGGTGA TGGATTTAGA AGAAGCCATT CCCCTGGACT GCTGTCGTCT TGTTAAGTAT 
GATGAGTTTC ATGATTATCT AGAGAGGTCA TATGAAGGAG AAGAGGATAC TCCAATGGGA 
CTTCTACTTG GTGGAGTCAA ATCAACATAC ATGTTTGATC TGCTCTTGGA AATCAAAAAA 
CCTGATCAGG TTTTTCAGTC TTATAAACCT GGTGAGGTGA TGGTGAAGGT CCATGTGGTT 
GATATAAAGG CAGAGTCTGT TGCTCCTCCA TTTAGTGTAC GAGCTTATTT AAATCAAACA 
GTCGCCGAGT TTAAGCAACT TATTTCAAAG ACTATACAGC TGCCTGCTGA AACGATGAGA 
ATAGTTCTAG AACGCTGCTA CAATGATTTG CGTCTTCTCA ATGTGCCCAG TAAAACACTG 
AAGGCTGAAG GCTTCTTTAG AAGTAACAAG GTATTTGTCG AAAGTTCTGA GTCTTTGGAT 
CACCAGATGG CCTTTACAGA TTCTCATTTG TGGAAATTGC TGGATCGACA TGCAAATACA 
ATCAGACTGT TTGTTTTATT ACCTGAACAA TCTCCAGGAT CTCATTCTAA AAGGACAGTG 
TACCAGAAAG CTGGTGGGGA TTCTGGCAAC ATGGATGATG CTTGTGAACG AGTTAAAGGA 
CCTCTAGGTA GCCTAAAATC TGTAGAGGCT ATCTTAGAAG AAAGTACTGA AAAGCTTAAA 
AGCTTGTCTC TTCGACAGCA ACAGGATGGA GATAATGGAG ACAGTAGCAA GAGTACAGAG 
ACAAGTGATT TTGAAAACAT TGAATCACCT CTAAATGAGA GAGACTCTTC AGGATCAGTA 
GATACCAGAG AACTTGAACA GCAGATTCCC ACTTCTGATC CAGAAAATTT CCAGTCTGAA 
GAGCGATCCG ACTCTGATGT GAACAATGAC AGGAGTACAA GCTCAGTGGA CAGTGACATT 
CTTAGTTCCA GTCACAGTAG TGACACTTTG TGCAATGCAG ACAGTGCCCC GATCCCTTTG 
GCTAATGGAC TTGACTCTCA TAGTATCACA AGTAGTAGAA GATCTAAGGC AAATGAAGGA 
AAAAAGGAGA CCTGGGATAC AGCTGAAGAA GACTCTGGAA CTGACAGTGA ATATGATGAA 
AGTGGCAAGA GTAGGGGAGA GACACAGTAC ATATATTTCA AAGCAGAATC CTATCCTGCA 
GATGAAGGTT CTGGGGAAGG ACATAAATGG TTGATGGTGC ATGTTGATAA AAGAATTACA 
CTGGCTACCT TCAAACAACA GTTGGAACCC TTTGTTGGAG CTCTGTCTTC TCACTTCAAA 
GTTTTTCGAG TATATGCCAG CAATCAAGAG TTTGAAAGCA TCCGACTGAA TGAAACACTT 
TCATCATTTT CAGATGACAA CAAGATTACA ATTAGATTGG GGAGAGCACT TAAGAAGGGA 
GAGTACAGAG TCAAAGTGTA CCAGCTTTTA GTAAATGAGC AAGAGCCCTG TAGGTTTCTG 
CTTGATGCGG TTTTTGCTAA AGGCATGACT GTTCGACAGT CTAAAGAAGA GCTCATCCCC 
CAGCTCAGGG AGCAGTGTGG GCTAGAAGTC AGCATTGACA GGTTTCGTCT AAGGAAAAAA 
ACATGGAAGA ATCCTGGCAC AGTCTTTTTG GATTATCATG TCTATGAAGA GGATATTAAT 
ATTTCCAGCA ACTGGGAGGT TTTCCTTGAA GTTCTTGATG AAGTGGAGAG GATGAAATCC 
ATGTCACAAT TGGCTGTACT GGCCAGAAGA TGGAGACCAA CAGAGCTGAT ACTGGACCCC 
TTCCAAGAGA TAGTGTTGGA AAGCAGTAGT GTTGATGAAC TGAGAGAGAA GCTCTGCGAA 
ATCAGTGGGA TACCTTTGGA AGATGTGGAA TTTGCAAAGG GAAGAGGAAC ATTTCCCTGT 
GACATGTCTG TCCTAGAAAT TCACCAGGAT TTGGATTGGA ATCCCAAAGT TTCTACATTG 
AATGTGTGGC CCCTTTATAT CTGTGATGAT GGTGCGGTGA TATTTTATAG GGATAAAACT 
GAAGAATTAT TGGAATTAAC AGATGAGCAG AGAAATGAAC TAATGAAAAA GGAAAGCAGC 
CGGCTCCAGA AAACTGGACA TCGTGTAACC TACTCTCCTC GTAAAGAAAA GGCACTAAAA 
ATTTATCTGG ACGGATCATC AAATAAAGAT TTGACTCAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012407971.1
CDS	328..4392
Translation	MVPGEENQLVPKEIENAAEEPRVLCIIQDTTNSKTVNERITLNLPASTPLRKLFEDVANKVGYVNGTFDLVWGNGINATDMPPLDHTSEKSLLDTNFEPGKKNFLHLTDKDGEQPQIAEDTIAADDSVQDRFIGPLPREGSVGSTNDYVGQSYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEESEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGASQAFASVEEALQAFIQPEILDGPNQYFCEQCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMSTFIDVEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSNDDGVDEGICLESNSGTEKITKPGLEKNSLLYELFSVMVHSGSAAGGHYYACIKSFSDEQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPSRNAKFLEVKDYPEHIKNLVRKEKELEEQEKKQREIERNTCKIKLFCMHPVKQVMMENKLEVHKDKTLKEAVEIAYKVMDLEEAIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLEIKKPDQVFQSYKPGEVMVKVHVVDIKAESVAPPFSVRAYLNQTVAEFKQLISKTIQLPAETMRIVLERCYNDLRLLNVPSKTLKAEGFFRSNKVFVESSESLDHQMAFTDSHLWKLLDRHANTIRLFVLLPEQSPGSHSKRTVYQKAGGDSGNMDDACERVKGPLGSLKSVEAILEESTEKLKSLSLRQQQDGDNGDSSKSTETSDFENIESPLNERDSSGSVDTRELEQQIPTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSAPIPLANGLDSHSITSSRRSKANEGKKETWDTAEEDSGTDSEYDESGKSRGETQYIYFKAESYPADEGSGEGHKWLMVHVDKRITLATFKQQLEPFVGALSSHFKVFRVYASNQEFESIRLNETLSSFSDDNKITIRLGRALKKGEYRVKVYQLLVNEQEPCRFLLDAVFAKGMTVRQSKEELIPQLREQCGLEVSIDRFRLRKKTWKNPGTVFLDYHVYEEDINISSNWEVFLEVLDEVERMKSMSQLAVLARRWRPTELILDPFQEIVLESSSVDELREKLCEISGIPLEDVEFAKGRGTFPCDMSVLEIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDKTEELLELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGSSNKDLTQD

Target ORF information:

RefSeq Version	XM_012552517.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012552517.2

ORF Insert Sequence:

ATGGTCCCGG GCGAAGAGAA CCAGCTAGTC CCGAAGGAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTTT TATGTATTAT ACAAGATACT ACTAATTCAA AGACAGTCAA TGAACGAATC 
ACTTTAAATT TACCAGCTTC TACTCCACTG AGGAAACTAT TTGAAGATGT TGCCAACAAA 
GTGGGCTATG TAAATGGAAC CTTTGATTTG GTATGGGGAA ATGGAATCAA TGCTACTGAC 
ATGCCTCCTT TGGATCACAC CAGTGAAAAA TCTCTTCTTG ACACTAACTT TGAACCTGGG 
AAGAAAAACT TTCTGCATTT GACAGATAAA GATGGTGAAC AGCCTCAGAT AGCAGAGGAT 
ACCATTGCAG CAGATGACAG TGTGCAAGAT AGGTTTATAG GCCCTCTTCC AAGAGAAGGT 
TCTGTAGGCT CTACCAATGA TTATGTCGGT CAAAGCTATT CATATTCTTC AATCTTGAAT 
AAATCAGAAA CAGGATATGT GGGATTAGTA AATCAAGCAA TGACTTGCTA TTTGAACAGC 
CTTTTGCAAA CACTTTTCAT GACTCCTGAA TTCAGGAATG CACTGTATAA GTGGGAATTT 
GAGGAATCTG AAGAAGATCC TGTGACAAGC ATTCCATACC AGCTTCAGAG ACTCTTTGTT 
TTATTACAAA CCAGCAAAAA GAGAGCAATT GAAACGACTG ATGTTACAAG AAGCTTTGGA 
TGGGATAGTA GTGAGGCTTG GCAACAACAT GATGTACAAG AACTTTGTAG AGTCATGTTT 
GATGCATTGG AACAGAAATG GAAGCAAACA GAGCAGGCCG ATCTTATAAA TGAACTGTAT 
CAAGGAAAGC TGAAGGACTA CGTGAGATGT CTGGAATGTG GTTATGAGGG CTGGAGAATC 
GACACATATC TTGACATTCC ATTGGTCATC CGACCTTATG GTGCCAGCCA GGCATTTGCT 
AGTGTGGAAG AAGCATTACA AGCATTTATA CAGCCAGAAA TCCTGGATGG CCCCAACCAG 
TACTTTTGTG AGCAGTGTAA AAAGAAGTGT GATGCACGGA AGGGCCTTCG TTTTTTGCAT 
TTCCCATATC TGCTGACTCT CCAGTTGAAA AGGTTTGACT TTGATTACAC CACCATGCAC 
AGGATTAAAT TAAATGACCG GATGACTTTC CCAGAAGAGC TAGATATGAG TACATTTATT 
GATGTTGAAG ATGAGAAGTC TCCTCAGACT GAGAGTTGTA CCGACAGTGG AGCTGAAAAT 
GAAGGCAGTT GTCACAGTGA TCAGATGAGC AACGATTTCT CAAATGATGA TGGGGTCGAT 
GAAGGAATCT GCCTTGAAAG TAATAGTGGA ACTGAAAAGA TCACAAAACC TGGTCTTGAA 
AAGAATTCCT TGCTATACGA ACTCTTTTCT GTCATGGTTC ATTCAGGAAG TGCAGCTGGT 
GGTCATTATT ATGCGTGCAT AAAATCATTC AGTGATGAGC AGTGGTACAG CTTCAATGAT 
CAGCATGTTA GTCGGATAAC CCAAGAGGAC ATTAAGAAGA CTCATGGCGG ATCTTCAGGA 
AGTCGAGGAT ATTATTCTAG TGCCTTTGCC AGCTCCACAA ATGCATATAT GCTTATATAT 
AGATTGAAGG ATCCTTCAAG AAATGCAAAA TTTTTAGAAG TGAAAGATTA CCCAGAGCAC 
ATTAAGAATT TGGTGCGAAA AGAGAAAGAA CTGGAAGAGC AAGAAAAAAA GCAACGTGAA 
ATTGAGCGAA ATACCTGCAA GATAAAATTG TTCTGCATGC ATCCTGTGAA ACAAGTGATG 
ATGGAAAATA AATTGGAGGT TCATAAAGAT AAGACCTTAA AAGAAGCAGT AGAAATAGCT 
TATAAGGTGA TGGATTTAGA AGAAGCCATT CCCCTGGACT GCTGTCGTCT TGTTAAGTAT 
GATGAGTTTC ATGATTATCT AGAGAGGTCA TATGAAGGAG AAGAGGATAC TCCAATGGGA 
CTTCTACTTG GTGGAGTCAA ATCAACATAC ATGTTTGATC TGCTCTTGGA AATCAAAAAA 
CCTGATCAGG TTTTTCAGTC TTATAAACCT GGTGAGGTGA TGGTGAAGGT CCATGTGGTT 
GATATAAAGG CAGAGTCTGT TGCTCCTCCA TTTAGTGTAC GAGCTTATTT AAATCAAACA 
GTCGCCGAGT TTAAGCAACT TATTTCAAAG ACTATACAGC TGCCTGCTGA AACGATGAGA 
ATAGTTCTAG AACGCTGCTA CAATGATTTG CGTCTTCTCA ATGTGCCCAG TAAAACACTG 
AAGGCTGAAG GCTTCTTTAG AAGTAACAAG GTATTTGTCG AAAGTTCTGA GTCTTTGGAT 
CACCAGATGG CCTTTACAGA TTCTCATTTG TGGAAATTGC TGGATCGACA TGCAAATACA 
ATCAGACTGT TTGTTTTATT ACCTGAACAA TCTCCAGGAT CTCATTCTAA AAGGACAGTG 
TACCAGAAAG CTGGTGGGGA TTCTGGCAAC ATGGATGATG CTTGTGAACG AGTTAAAGGA 
CCTCTAGGTA GCCTAAAATC TGTAGAGGCT ATCTTAGAAG AAAGTACTGA AAAGCTTAAA 
AGCTTGTCTC TTCGACAGCA ACAGGATGGA GATAATGGAG ACAGTAGCAA GAGTACAGAG 
ACAAGTGATT TTGAAAACAT TGAATCACCT CTAAATGAGA GAGACTCTTC AGGATCAGTA 
GATACCAGAG AACTTGAACA GCAGATTCCC ACTTCTGATC CAGAAAATTT CCAGTCTGAA 
GAGCGATCCG ACTCTGATGT GAACAATGAC AGGAGTACAA GCTCAGTGGA CAGTGACATT 
CTTAGTTCCA GTCACAGTAG TGACACTTTG TGCAATGCAG ACAGTGCCCC GATCCCTTTG 
GCTAATGGAC TTGACTCTCA TAGTATCACA AGTAGTAGAA GATCTAAGGC AAATGAAGGA 
AAAAAGGAGA CCTGGGATAC AGCTGAAGAA GACTCTGGAA CTGACAGTGA ATATGATGAA 
AGTGGCAAGA GTAGGGGAGA GACACAGTAC ATATATTTCA AAGCAGAATC CTATCCTGCA 
GATGAAGGTT CTGGGGAAGG ACATAAATGG TTGATGGTGC ATGTTGATAA AAGAATTACA 
CTGGCTACCT TCAAACAACA GTTGGAACCC TTTGTTGGAG CTCTGTCTTC TCACTTCAAA 
GTTTTTCGAG TATATGCCAG CAATCAAGAG TTTGAAAGCA TCCGACTGAA TGAAACACTT 
TCATCATTTT CAGATGACAA CAAGATTACA ATTAGATTGG GGAGAGCACT TAAGAAGGGA 
GAGTACAGAG TCAAAGTGTA CCAGCTTTTA GTAAATGAGC AAGAGCCCTG TAGGTTTCTG 
CTTGATGCGG TTTTTGCTAA AGGCATGACT GTTCGACAGT CTAAAGAAGA GCTCATCCCC 
CAGCTCAGGG AGCAGTGTGG GCTAGAAGTC AGCATTGACA GGTTTCGTCT AAGGAAAAAA 
ACATGGAAGA ATCCTGGCAC AGTCTTTTTG GATTATCATG TCTATGAAGA GGATATTAAT 
ATTTCCAGCA ACTGGGAGGT TTTCCTTGAA GTTCTTGATG AAGTGGAGAG GATGAAATCC 
ATGTCACAAT TGGCTGTACT GGCCAGAAGA TGGAGACCAA CAGAGCTGAT ACTGGACCCC 
TTCCAAGAGA TAGTGTTGGA AAGCAGTAGT GTTGATGAAC TGAGAGAGAA GCTCTGCGAA 
ATCAGTGGGA TACCTTTGGA AGATGTGGAA TTTGCAAAGG GAAGAGGAAC ATTTCCCTGT 
GACATGTCTG TCCTAGAAAT TCACCAGGAT TTGGATTGGA ATCCCAAAGT TTCTACATTG 
AATGTGTGGC CCCTTTATAT CTGTGATGAT GGTGCGGTGA TATTTTATAG GGATAAAACT 
GAAGAATTAT TGGAATTAAC AGATGAGCAG AGAAATGAAC TAATGAAAAA GGAAAGCAGC 
CGGCTCCAGA AAACTGGACA TCGTGTAACC TACTCTCCTC GTAAAGAAAA GGCACTAAAA 
ATTTATCTGG ACGGATCATC AAATAAAGAT TTGACTCAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP47 ( XM_031944165.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031800025.1 Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X2, mRNA.

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CloneID	OSa135893
Clone ID Related Accession (Same CDS sequence)	XM_012552517.1 , XM_012552517.2 , XM_031944165.1
Accession Version	XM_031944165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045431.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCGG GCGAAGAGAA CCAGCTAGTC CCGAAGGAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTTT TATGTATTAT ACAAGATACT ACTAATTCAA AGACAGTCAA TGAACGAATC 
ACTTTAAATT TACCAGCTTC TACTCCACTG AGGAAACTAT TTGAAGATGT TGCCAACAAA 
GTGGGCTATG TAAATGGAAC CTTTGATTTG GTATGGGGAA ATGGAATCAA TGCTACTGAC 
ATGCCTCCTT TGGATCACAC CAGTGAAAAA TCTCTTCTTG ACACTAACTT TGAACCTGGG 
AAGAAAAACT TTCTGCATTT GACAGATAAA GATGGTGAAC AGCCTCAGAT AGCAGAGGAT 
ACCATTGCAG CAGATGACAG TGTGCAAGAT AGGTTTATAG GCCCTCTTCC AAGAGAAGGT 
TCTGTAGGCT CTACCAATGA TTATGTCGGT CAAAGCTATT CATATTCTTC AATCTTGAAT 
AAATCAGAAA CAGGATATGT GGGATTAGTA AATCAAGCAA TGACTTGCTA TTTGAACAGC 
CTTTTGCAAA CACTTTTCAT GACTCCTGAA TTCAGGAATG CACTGTATAA GTGGGAATTT 
GAGGAATCTG AAGAAGATCC TGTGACAAGC ATTCCATACC AGCTTCAGAG ACTCTTTGTT 
TTATTACAAA CCAGCAAAAA GAGAGCAATT GAAACGACTG ATGTTACAAG AAGCTTTGGA 
TGGGATAGTA GTGAGGCTTG GCAACAACAT GATGTACAAG AACTTTGTAG AGTCATGTTT 
GATGCATTGG AACAGAAATG GAAGCAAACA GAGCAGGCCG ATCTTATAAA TGAACTGTAT 
CAAGGAAAGC TGAAGGACTA CGTGAGATGT CTGGAATGTG GTTATGAGGG CTGGAGAATC 
GACACATATC TTGACATTCC ATTGGTCATC CGACCTTATG GTGCCAGCCA GGCATTTGCT 
AGTGTGGAAG AAGCATTACA AGCATTTATA CAGCCAGAAA TCCTGGATGG CCCCAACCAG 
TACTTTTGTG AGCAGTGTAA AAAGAAGTGT GATGCACGGA AGGGCCTTCG TTTTTTGCAT 
TTCCCATATC TGCTGACTCT CCAGTTGAAA AGGTTTGACT TTGATTACAC CACCATGCAC 
AGGATTAAAT TAAATGACCG GATGACTTTC CCAGAAGAGC TAGATATGAG TACATTTATT 
GATGTTGAAG ATGAGAAGTC TCCTCAGACT GAGAGTTGTA CCGACAGTGG AGCTGAAAAT 
GAAGGCAGTT GTCACAGTGA TCAGATGAGC AACGATTTCT CAAATGATGA TGGGGTCGAT 
GAAGGAATCT GCCTTGAAAG TAATAGTGGA ACTGAAAAGA TCACAAAACC TGGTCTTGAA 
AAGAATTCCT TGCTATACGA ACTCTTTTCT GTCATGGTTC ATTCAGGAAG TGCAGCTGGT 
GGTCATTATT ATGCGTGCAT AAAATCATTC AGTGATGAGC AGTGGTACAG CTTCAATGAT 
CAGCATGTTA GTCGGATAAC CCAAGAGGAC ATTAAGAAGA CTCATGGCGG ATCTTCAGGA 
AGTCGAGGAT ATTATTCTAG TGCCTTTGCC AGCTCCACAA ATGCATATAT GCTTATATAT 
AGATTGAAGG ATCCTTCAAG AAATGCAAAA TTTTTAGAAG TGAAAGATTA CCCAGAGCAC 
ATTAAGAATT TGGTGCGAAA AGAGAAAGAA CTGGAAGAGC AAGAAAAAAA GCAACGTGAA 
ATTGAGCGAA ATACCTGCAA GATAAAATTG TTCTGCATGC ATCCTGTGAA ACAAGTGATG 
ATGGAAAATA AATTGGAGGT TCATAAAGAT AAGACCTTAA AAGAAGCAGT AGAAATAGCT 
TATAAGGTGA TGGATTTAGA AGAAGCCATT CCCCTGGACT GCTGTCGTCT TGTTAAGTAT 
GATGAGTTTC ATGATTATCT AGAGAGGTCA TATGAAGGAG AAGAGGATAC TCCAATGGGA 
CTTCTACTTG GTGGAGTCAA ATCAACATAC ATGTTTGATC TGCTCTTGGA AATCAAAAAA 
CCTGATCAGG TTTTTCAGTC TTATAAACCT GGTGAGGTGA TGGTGAAGGT CCATGTGGTT 
GATATAAAGG CAGAGTCTGT TGCTCCTCCA TTTAGTGTAC GAGCTTATTT AAATCAAACA 
GTCGCCGAGT TTAAGCAACT TATTTCAAAG ACTATACAGC TGCCTGCTGA AACGATGAGA 
ATAGTTCTAG AACGCTGCTA CAATGATTTG CGTCTTCTCA ATGTGCCCAG TAAAACACTG 
AAGGCTGAAG GCTTCTTTAG AAGTAACAAG GTATTTGTCG AAAGTTCTGA GTCTTTGGAT 
CACCAGATGG CCTTTACAGA TTCTCATTTG TGGAAATTGC TGGATCGACA TGCAAATACA 
ATCAGACTGT TTGTTTTATT ACCTGAACAA TCTCCAGGAT CTCATTCTAA AAGGACAGTG 
TACCAGAAAG CTGGTGGGGA TTCTGGCAAC ATGGATGATG CTTGTGAACG AGTTAAAGGA 
CCTCTAGGTA GCCTAAAATC TGTAGAGGCT ATCTTAGAAG AAAGTACTGA AAAGCTTAAA 
AGCTTGTCTC TTCGACAGCA ACAGGATGGA GATAATGGAG ACAGTAGCAA GAGTACAGAG 
ACAAGTGATT TTGAAAACAT TGAATCACCT CTAAATGAGA GAGACTCTTC AGGATCAGTA 
GATACCAGAG AACTTGAACA GCAGATTCCC ACTTCTGATC CAGAAAATTT CCAGTCTGAA 
GAGCGATCCG ACTCTGATGT GAACAATGAC AGGAGTACAA GCTCAGTGGA CAGTGACATT 
CTTAGTTCCA GTCACAGTAG TGACACTTTG TGCAATGCAG ACAGTGCCCC GATCCCTTTG 
GCTAATGGAC TTGACTCTCA TAGTATCACA AGTAGTAGAA GATCTAAGGC AAATGAAGGA 
AAAAAGGAGA CCTGGGATAC AGCTGAAGAA GACTCTGGAA CTGACAGTGA ATATGATGAA 
AGTGGCAAGA GTAGGGGAGA GACACAGTAC ATATATTTCA AAGCAGAATC CTATCCTGCA 
GATGAAGGTT CTGGGGAAGG ACATAAATGG TTGATGGTGC ATGTTGATAA AAGAATTACA 
CTGGCTACCT TCAAACAACA GTTGGAACCC TTTGTTGGAG CTCTGTCTTC TCACTTCAAA 
GTTTTTCGAG TATATGCCAG CAATCAAGAG TTTGAAAGCA TCCGACTGAA TGAAACACTT 
TCATCATTTT CAGATGACAA CAAGATTACA ATTAGATTGG GGAGAGCACT TAAGAAGGGA 
GAGTACAGAG TCAAAGTGTA CCAGCTTTTA GTAAATGAGC AAGAGCCCTG TAGGTTTCTG 
CTTGATGCGG TTTTTGCTAA AGGCATGACT GTTCGACAGT CTAAAGAAGA GCTCATCCCC 
CAGCTCAGGG AGCAGTGTGG GCTAGAAGTC AGCATTGACA GGTTTCGTCT AAGGAAAAAA 
ACATGGAAGA ATCCTGGCAC AGTCTTTTTG GATTATCATG TCTATGAAGA GGATATTAAT 
ATTTCCAGCA ACTGGGAGGT TTTCCTTGAA GTTCTTGATG AAGTGGAGAG GATGAAATCC 
ATGTCACAAT TGGCTGTACT GGCCAGAAGA TGGAGACCAA CAGAGCTGAT ACTGGACCCC 
TTCCAAGAGA TAGTGTTGGA AAGCAGTAGT GTTGATGAAC TGAGAGAGAA GCTCTGCGAA 
ATCAGTGGGA TACCTTTGGA AGATGTGGAA TTTGCAAAGG GAAGAGGAAC ATTTCCCTGT 
GACATGTCTG TCCTAGAAAT TCACCAGGAT TTGGATTGGA ATCCCAAAGT TTCTACATTG 
AATGTGTGGC CCCTTTATAT CTGTGATGAT GGTGCGGTGA TATTTTATAG GGATAAAACT 
GAAGAATTAT TGGAATTAAC AGATGAGCAG AGAAATGAAC TAATGAAAAA GGAAAGCAGC 
CGGCTCCAGA AAACTGGACA TCGTGTAACC TACTCTCCTC GTAAAGAAAA GGCACTAAAA 
ATTTATCTGG ACGGATCATC AAATAAAGAT TTGACTCAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031800025.1
CDS	200..4264
Translation	MVPGEENQLVPKEIENAAEEPRVLCIIQDTTNSKTVNERITLNLPASTPLRKLFEDVANKVGYVNGTFDLVWGNGINATDMPPLDHTSEKSLLDTNFEPGKKNFLHLTDKDGEQPQIAEDTIAADDSVQDRFIGPLPREGSVGSTNDYVGQSYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEESEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGASQAFASVEEALQAFIQPEILDGPNQYFCEQCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMSTFIDVEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSNDDGVDEGICLESNSGTEKITKPGLEKNSLLYELFSVMVHSGSAAGGHYYACIKSFSDEQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPSRNAKFLEVKDYPEHIKNLVRKEKELEEQEKKQREIERNTCKIKLFCMHPVKQVMMENKLEVHKDKTLKEAVEIAYKVMDLEEAIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLEIKKPDQVFQSYKPGEVMVKVHVVDIKAESVAPPFSVRAYLNQTVAEFKQLISKTIQLPAETMRIVLERCYNDLRLLNVPSKTLKAEGFFRSNKVFVESSESLDHQMAFTDSHLWKLLDRHANTIRLFVLLPEQSPGSHSKRTVYQKAGGDSGNMDDACERVKGPLGSLKSVEAILEESTEKLKSLSLRQQQDGDNGDSSKSTETSDFENIESPLNERDSSGSVDTRELEQQIPTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSAPIPLANGLDSHSITSSRRSKANEGKKETWDTAEEDSGTDSEYDESGKSRGETQYIYFKAESYPADEGSGEGHKWLMVHVDKRITLATFKQQLEPFVGALSSHFKVFRVYASNQEFESIRLNETLSSFSDDNKITIRLGRALKKGEYRVKVYQLLVNEQEPCRFLLDAVFAKGMTVRQSKEELIPQLREQCGLEVSIDRFRLRKKTWKNPGTVFLDYHVYEEDINISSNWEVFLEVLDEVERMKSMSQLAVLARRWRPTELILDPFQEIVLESSSVDELREKLCEISGIPLEDVEFAKGRGTFPCDMSVLEIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDKTEELLELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGSSNKDLTQD

Target ORF information:

RefSeq Version	XM_031944165.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031944165.1

ORF Insert Sequence:

ATGGTCCCGG GCGAAGAGAA CCAGCTAGTC CCGAAGGAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTTT TATGTATTAT ACAAGATACT ACTAATTCAA AGACAGTCAA TGAACGAATC 
ACTTTAAATT TACCAGCTTC TACTCCACTG AGGAAACTAT TTGAAGATGT TGCCAACAAA 
GTGGGCTATG TAAATGGAAC CTTTGATTTG GTATGGGGAA ATGGAATCAA TGCTACTGAC 
ATGCCTCCTT TGGATCACAC CAGTGAAAAA TCTCTTCTTG ACACTAACTT TGAACCTGGG 
AAGAAAAACT TTCTGCATTT GACAGATAAA GATGGTGAAC AGCCTCAGAT AGCAGAGGAT 
ACCATTGCAG CAGATGACAG TGTGCAAGAT AGGTTTATAG GCCCTCTTCC AAGAGAAGGT 
TCTGTAGGCT CTACCAATGA TTATGTCGGT CAAAGCTATT CATATTCTTC AATCTTGAAT 
AAATCAGAAA CAGGATATGT GGGATTAGTA AATCAAGCAA TGACTTGCTA TTTGAACAGC 
CTTTTGCAAA CACTTTTCAT GACTCCTGAA TTCAGGAATG CACTGTATAA GTGGGAATTT 
GAGGAATCTG AAGAAGATCC TGTGACAAGC ATTCCATACC AGCTTCAGAG ACTCTTTGTT 
TTATTACAAA CCAGCAAAAA GAGAGCAATT GAAACGACTG ATGTTACAAG AAGCTTTGGA 
TGGGATAGTA GTGAGGCTTG GCAACAACAT GATGTACAAG AACTTTGTAG AGTCATGTTT 
GATGCATTGG AACAGAAATG GAAGCAAACA GAGCAGGCCG ATCTTATAAA TGAACTGTAT 
CAAGGAAAGC TGAAGGACTA CGTGAGATGT CTGGAATGTG GTTATGAGGG CTGGAGAATC 
GACACATATC TTGACATTCC ATTGGTCATC CGACCTTATG GTGCCAGCCA GGCATTTGCT 
AGTGTGGAAG AAGCATTACA AGCATTTATA CAGCCAGAAA TCCTGGATGG CCCCAACCAG 
TACTTTTGTG AGCAGTGTAA AAAGAAGTGT GATGCACGGA AGGGCCTTCG TTTTTTGCAT 
TTCCCATATC TGCTGACTCT CCAGTTGAAA AGGTTTGACT TTGATTACAC CACCATGCAC 
AGGATTAAAT TAAATGACCG GATGACTTTC CCAGAAGAGC TAGATATGAG TACATTTATT 
GATGTTGAAG ATGAGAAGTC TCCTCAGACT GAGAGTTGTA CCGACAGTGG AGCTGAAAAT 
GAAGGCAGTT GTCACAGTGA TCAGATGAGC AACGATTTCT CAAATGATGA TGGGGTCGAT 
GAAGGAATCT GCCTTGAAAG TAATAGTGGA ACTGAAAAGA TCACAAAACC TGGTCTTGAA 
AAGAATTCCT TGCTATACGA ACTCTTTTCT GTCATGGTTC ATTCAGGAAG TGCAGCTGGT 
GGTCATTATT ATGCGTGCAT AAAATCATTC AGTGATGAGC AGTGGTACAG CTTCAATGAT 
CAGCATGTTA GTCGGATAAC CCAAGAGGAC ATTAAGAAGA CTCATGGCGG ATCTTCAGGA 
AGTCGAGGAT ATTATTCTAG TGCCTTTGCC AGCTCCACAA ATGCATATAT GCTTATATAT 
AGATTGAAGG ATCCTTCAAG AAATGCAAAA TTTTTAGAAG TGAAAGATTA CCCAGAGCAC 
ATTAAGAATT TGGTGCGAAA AGAGAAAGAA CTGGAAGAGC AAGAAAAAAA GCAACGTGAA 
ATTGAGCGAA ATACCTGCAA GATAAAATTG TTCTGCATGC ATCCTGTGAA ACAAGTGATG 
ATGGAAAATA AATTGGAGGT TCATAAAGAT AAGACCTTAA AAGAAGCAGT AGAAATAGCT 
TATAAGGTGA TGGATTTAGA AGAAGCCATT CCCCTGGACT GCTGTCGTCT TGTTAAGTAT 
GATGAGTTTC ATGATTATCT AGAGAGGTCA TATGAAGGAG AAGAGGATAC TCCAATGGGA 
CTTCTACTTG GTGGAGTCAA ATCAACATAC ATGTTTGATC TGCTCTTGGA AATCAAAAAA 
CCTGATCAGG TTTTTCAGTC TTATAAACCT GGTGAGGTGA TGGTGAAGGT CCATGTGGTT 
GATATAAAGG CAGAGTCTGT TGCTCCTCCA TTTAGTGTAC GAGCTTATTT AAATCAAACA 
GTCGCCGAGT TTAAGCAACT TATTTCAAAG ACTATACAGC TGCCTGCTGA AACGATGAGA 
ATAGTTCTAG AACGCTGCTA CAATGATTTG CGTCTTCTCA ATGTGCCCAG TAAAACACTG 
AAGGCTGAAG GCTTCTTTAG AAGTAACAAG GTATTTGTCG AAAGTTCTGA GTCTTTGGAT 
CACCAGATGG CCTTTACAGA TTCTCATTTG TGGAAATTGC TGGATCGACA TGCAAATACA 
ATCAGACTGT TTGTTTTATT ACCTGAACAA TCTCCAGGAT CTCATTCTAA AAGGACAGTG 
TACCAGAAAG CTGGTGGGGA TTCTGGCAAC ATGGATGATG CTTGTGAACG AGTTAAAGGA 
CCTCTAGGTA GCCTAAAATC TGTAGAGGCT ATCTTAGAAG AAAGTACTGA AAAGCTTAAA 
AGCTTGTCTC TTCGACAGCA ACAGGATGGA GATAATGGAG ACAGTAGCAA GAGTACAGAG 
ACAAGTGATT TTGAAAACAT TGAATCACCT CTAAATGAGA GAGACTCTTC AGGATCAGTA 
GATACCAGAG AACTTGAACA GCAGATTCCC ACTTCTGATC CAGAAAATTT CCAGTCTGAA 
GAGCGATCCG ACTCTGATGT GAACAATGAC AGGAGTACAA GCTCAGTGGA CAGTGACATT 
CTTAGTTCCA GTCACAGTAG TGACACTTTG TGCAATGCAG ACAGTGCCCC GATCCCTTTG 
GCTAATGGAC TTGACTCTCA TAGTATCACA AGTAGTAGAA GATCTAAGGC AAATGAAGGA 
AAAAAGGAGA CCTGGGATAC AGCTGAAGAA GACTCTGGAA CTGACAGTGA ATATGATGAA 
AGTGGCAAGA GTAGGGGAGA GACACAGTAC ATATATTTCA AAGCAGAATC CTATCCTGCA 
GATGAAGGTT CTGGGGAAGG ACATAAATGG TTGATGGTGC ATGTTGATAA AAGAATTACA 
CTGGCTACCT TCAAACAACA GTTGGAACCC TTTGTTGGAG CTCTGTCTTC TCACTTCAAA 
GTTTTTCGAG TATATGCCAG CAATCAAGAG TTTGAAAGCA TCCGACTGAA TGAAACACTT 
TCATCATTTT CAGATGACAA CAAGATTACA ATTAGATTGG GGAGAGCACT TAAGAAGGGA 
GAGTACAGAG TCAAAGTGTA CCAGCTTTTA GTAAATGAGC AAGAGCCCTG TAGGTTTCTG 
CTTGATGCGG TTTTTGCTAA AGGCATGACT GTTCGACAGT CTAAAGAAGA GCTCATCCCC 
CAGCTCAGGG AGCAGTGTGG GCTAGAAGTC AGCATTGACA GGTTTCGTCT AAGGAAAAAA 
ACATGGAAGA ATCCTGGCAC AGTCTTTTTG GATTATCATG TCTATGAAGA GGATATTAAT 
ATTTCCAGCA ACTGGGAGGT TTTCCTTGAA GTTCTTGATG AAGTGGAGAG GATGAAATCC 
ATGTCACAAT TGGCTGTACT GGCCAGAAGA TGGAGACCAA CAGAGCTGAT ACTGGACCCC 
TTCCAAGAGA TAGTGTTGGA AAGCAGTAGT GTTGATGAAC TGAGAGAGAA GCTCTGCGAA 
ATCAGTGGGA TACCTTTGGA AGATGTGGAA TTTGCAAAGG GAAGAGGAAC ATTTCCCTGT 
GACATGTCTG TCCTAGAAAT TCACCAGGAT TTGGATTGGA ATCCCAAAGT TTCTACATTG 
AATGTGTGGC CCCTTTATAT CTGTGATGAT GGTGCGGTGA TATTTTATAG GGATAAAACT 
GAAGAATTAT TGGAATTAAC AGATGAGCAG AGAAATGAAC TAATGAAAAA GGAAAGCAGC 
CGGCTCCAGA AAACTGGACA TCGTGTAACC TACTCTCCTC GTAAAGAAAA GGCACTAAAA 
ATTTATCTGG ACGGATCATC AAATAAAGAT TTGACTCAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP47 ( XM_012552518.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012407972.1 Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa135894
Clone ID Related Accession (Same CDS sequence)	XM_012552518.1 , XM_012552518.2
Accession Version	XM_012552518.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003846892.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCGG GCGAAGAGAA CCAGCTAGTC CCGAAGGAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTTT TATGTATTAT ACAAGATACT ACTAATTCAA AGACAGTCAA TGAACGAATC 
ACTTTAAATT TACCAGCTTC TACTCCACTG AGGAAACTAT TTGAAGATGT TGCCAACAAA 
GTGGGCTATG TAAATGGAAC CTTTGATTTG GTATGGGGAA ATGGAATCAA TGCTACTGAC 
ATGCCTCCTT TGGATCACAC CAGTGAAAAA TCTCTTCTTG ACACTAACTT TGAACCTGGG 
AAGAAAAACT TTCTGCATTT GACAGATAAA GATGGTGAAC AGCCTCAGAT AGCAGAGGAT 
ACCATTGCAG CAGATGACAG TGTGCAAGAT AGGTTTATAG GCCCTCTTCC AAGAGAAGGT 
TCTGTAGGCT CTACCAATGA TTATGTCGGT CAAAGCTATT CATATTCTTC AATCTTGAAT 
AAATCAGAAA CAGGATATGT GGGATTAGTA AATCAAGCAA TGACTTGCTA TTTGAACAGC 
CTTTTGCAAA CACTTTTCAT GACTCCTGAA TTCAGGAATG CACTGTATAA GTGGGAATTT 
GAGGAATCTG AAGAAGATCC TGTGACAAGC ATTCCATACC AGCTTCAGAG ACTCTTTGTT 
TTATTACAAA CCAGCAAAAA GAGAGCAATT GAAACGACTG ATGTTACAAG AAGCTTTGGA 
TGGGATAGTA GTGAGGCTTG GCAACAACAT GATGTACAAG AACTTTGTAG AGTCATGTTT 
GATGCATTGG AACAGAAATG GAAGCAAACA GAGCAGGCCG ATCTTATAAA TGAACTGTAT 
CAAGGAAAGC TGAAGGACTA CGTGAGATGT CTGGAATGTG GTTATGAGGG CTGGAGAATC 
GACACATATC TTGACATTCC ATTGGTCATC CGACCTTATG GTGCCAGCCA GGCATTTGCT 
AGTGTGGAAG AAGCATTACA AGCATTTATA CAGCCAGAAA TCCTGGATGG CCCCAACCAG 
TACTTTTGTG AGCAGTGTAA AAAGAAGTGT GATGCACGGA AGGGCCTTCG TTTTTTGCAT 
TTCCCATATC TGCTGACTCT CCAGTTGAAA AGGTTTGACT TTGATTACAC CACCATGCAC 
AGGATTAAAT TAAATGACCG GATGACTTTC CCAGAAGAGC TAGATATGAG TACATTTATT 
GATGTTGAAG ATGAGAATTC CTTGCTATAC GAACTCTTTT CTGTCATGGT TCATTCAGGA 
AGTGCAGCTG GTGGTCATTA TTATGCGTGC ATAAAATCAT TCAGTGATGA GCAGTGGTAC 
AGCTTCAATG ATCAGCATGT TAGTCGGATA ACCCAAGAGG ACATTAAGAA GACTCATGGC 
GGATCTTCAG GAAGTCGAGG ATATTATTCT AGTGCCTTTG CCAGCTCCAC AAATGCATAT 
ATGCTTATAT ATAGATTGAA GGATCCTTCA AGAAATGCAA AATTTTTAGA AGTGAAAGAT 
TACCCAGAGC ACATTAAGAA TTTGGTGCGA AAAGAGAAAG AACTGGAAGA GCAAGAAAAA 
AAGCAACGTG AAATTGAGCG AAATACCTGC AAGATAAAAT TGTTCTGCAT GCATCCTGTG 
AAACAAGTGA TGATGGAAAA TAAATTGGAG GTTCATAAAG ATAAGACCTT AAAAGAAGCA 
GTAGAAATAG CTTATAAGGT GATGGATTTA GAAGAAGCCA TTCCCCTGGA CTGCTGTCGT 
CTTGTTAAGT ATGATGAGTT TCATGATTAT CTAGAGAGGT CATATGAAGG AGAAGAGGAT 
ACTCCAATGG GACTTCTACT TGGTGGAGTC AAATCAACAT ACATGTTTGA TCTGCTCTTG 
GAAATCAAAA AACCTGATCA GGTTTTTCAG TCTTATAAAC CTGGTGAGGT GATGGTGAAG 
GTCCATGTGG TTGATATAAA GGCAGAGTCT GTTGCTCCTC CATTTAGTGT ACGAGCTTAT 
TTAAATCAAA CAGTCGCCGA GTTTAAGCAA CTTATTTCAA AGACTATACA GCTGCCTGCT 
GAAACGATGA GAATAGTTCT AGAACGCTGC TACAATGATT TGCGTCTTCT CAATGTGCCC 
AGTAAAACAC TGAAGGCTGA AGGCTTCTTT AGAAGTAACA AGGTATTTGT CGAAAGTTCT 
GAGTCTTTGG ATCACCAGAT GGCCTTTACA GATTCTCATT TGTGGAAATT GCTGGATCGA 
CATGCAAATA CAATCAGACT GTTTGTTTTA TTACCTGAAC AATCTCCAGG ATCTCATTCT 
AAAAGGACAG TGTACCAGAA AGCTGGTGGG GATTCTGGCA ACATGGATGA TGCTTGTGAA 
CGAGTTAAAG GACCTCTAGG TAGCCTAAAA TCTGTAGAGG CTATCTTAGA AGAAAGTACT 
GAAAAGCTTA AAAGCTTGTC TCTTCGACAG CAACAGGATG GAGATAATGG AGACAGTAGC 
AAGAGTACAG AGACAAGTGA TTTTGAAAAC ATTGAATCAC CTCTAAATGA GAGAGACTCT 
TCAGGATCAG TAGATACCAG AGAACTTGAA CAGCAGATTC CCACTTCTGA TCCAGAAAAT 
TTCCAGTCTG AAGAGCGATC CGACTCTGAT GTGAACAATG ACAGGAGTAC AAGCTCAGTG 
GACAGTGACA TTCTTAGTTC CAGTCACAGT AGTGACACTT TGTGCAATGC AGACAGTGCC 
CCGATCCCTT TGGCTAATGG ACTTGACTCT CATAGTATCA CAAGTAGTAG AAGATCTAAG 
GCAAATGAAG GAAAAAAGGA GACCTGGGAT ACAGCTGAAG AAGACTCTGG AACTGACAGT 
GAATATGATG AAAGTGGCAA GAGTAGGGGA GAGACACAGT ACATATATTT CAAAGCAGAA 
TCCTATCCTG CAGATGAAGG TTCTGGGGAA GGACATAAAT GGTTGATGGT GCATGTTGAT 
AAAAGAATTA CACTGGCTAC CTTCAAACAA CAGTTGGAAC CCTTTGTTGG AGCTCTGTCT 
TCTCACTTCA AAGTTTTTCG AGTATATGCC AGCAATCAAG AGTTTGAAAG CATCCGACTG 
AATGAAACAC TTTCATCATT TTCAGATGAC AACAAGATTA CAATTAGATT GGGGAGAGCA 
CTTAAGAAGG GAGAGTACAG AGTCAAAGTG TACCAGCTTT TAGTAAATGA GCAAGAGCCC 
TGTAGGTTTC TGCTTGATGC GGTTTTTGCT AAAGGCATGA CTGTTCGACA GTCTAAAGAA 
GAGCTCATCC CCCAGCTCAG GGAGCAGTGT GGGCTAGAAG TCAGCATTGA CAGGTTTCGT 
CTAAGGAAAA AAACATGGAA GAATCCTGGC ACAGTCTTTT TGGATTATCA TGTCTATGAA 
GAGGATATTA ATATTTCCAG CAACTGGGAG GTTTTCCTTG AAGTTCTTGA TGAAGTGGAG 
AGGATGAAAT CCATGTCACA ATTGGCTGTA CTGGCCAGAA GATGGAGACC AACAGAGCTG 
ATACTGGACC CCTTCCAAGA GATAGTGTTG GAAAGCAGTA GTGTTGATGA ACTGAGAGAG 
AAGCTCTGCG AAATCAGTGG GATACCTTTG GAAGATGTGG AATTTGCAAA GGGAAGAGGA 
ACATTTCCCT GTGACATGTC TGTCCTAGAA ATTCACCAGG ATTTGGATTG GAATCCCAAA 
GTTTCTACAT TGAATGTGTG GCCCCTTTAT ATCTGTGATG ATGGTGCGGT GATATTTTAT 
AGGGATAAAA CTGAAGAATT ATTGGAATTA ACAGATGAGC AGAGAAATGA ACTAATGAAA 
AAGGAAAGCA GCCGGCTCCA GAAAACTGGA CATCGTGTAA CCTACTCTCC TCGTAAAGAA 
AAGGCACTAA AAATTTATCT GGACGGATCA TCAAATAAAG ATTTGACTCA AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012407972.1
CDS	308..4204
Translation	MVPGEENQLVPKEIENAAEEPRVLCIIQDTTNSKTVNERITLNLPASTPLRKLFEDVANKVGYVNGTFDLVWGNGINATDMPPLDHTSEKSLLDTNFEPGKKNFLHLTDKDGEQPQIAEDTIAADDSVQDRFIGPLPREGSVGSTNDYVGQSYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEESEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGASQAFASVEEALQAFIQPEILDGPNQYFCEQCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMSTFIDVEDENSLLYELFSVMVHSGSAAGGHYYACIKSFSDEQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPSRNAKFLEVKDYPEHIKNLVRKEKELEEQEKKQREIERNTCKIKLFCMHPVKQVMMENKLEVHKDKTLKEAVEIAYKVMDLEEAIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLEIKKPDQVFQSYKPGEVMVKVHVVDIKAESVAPPFSVRAYLNQTVAEFKQLISKTIQLPAETMRIVLERCYNDLRLLNVPSKTLKAEGFFRSNKVFVESSESLDHQMAFTDSHLWKLLDRHANTIRLFVLLPEQSPGSHSKRTVYQKAGGDSGNMDDACERVKGPLGSLKSVEAILEESTEKLKSLSLRQQQDGDNGDSSKSTETSDFENIESPLNERDSSGSVDTRELEQQIPTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSAPIPLANGLDSHSITSSRRSKANEGKKETWDTAEEDSGTDSEYDESGKSRGETQYIYFKAESYPADEGSGEGHKWLMVHVDKRITLATFKQQLEPFVGALSSHFKVFRVYASNQEFESIRLNETLSSFSDDNKITIRLGRALKKGEYRVKVYQLLVNEQEPCRFLLDAVFAKGMTVRQSKEELIPQLREQCGLEVSIDRFRLRKKTWKNPGTVFLDYHVYEEDINISSNWEVFLEVLDEVERMKSMSQLAVLARRWRPTELILDPFQEIVLESSSVDELREKLCEISGIPLEDVEFAKGRGTFPCDMSVLEIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDKTEELLELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGSSNKDLTQD

Target ORF information:

RefSeq Version	XM_012552518.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012552518.1

ORF Insert Sequence:

ATGGTCCCGG GCGAAGAGAA CCAGCTAGTC CCGAAGGAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTTT TATGTATTAT ACAAGATACT ACTAATTCAA AGACAGTCAA TGAACGAATC 
ACTTTAAATT TACCAGCTTC TACTCCACTG AGGAAACTAT TTGAAGATGT TGCCAACAAA 
GTGGGCTATG TAAATGGAAC CTTTGATTTG GTATGGGGAA ATGGAATCAA TGCTACTGAC 
ATGCCTCCTT TGGATCACAC CAGTGAAAAA TCTCTTCTTG ACACTAACTT TGAACCTGGG 
AAGAAAAACT TTCTGCATTT GACAGATAAA GATGGTGAAC AGCCTCAGAT AGCAGAGGAT 
ACCATTGCAG CAGATGACAG TGTGCAAGAT AGGTTTATAG GCCCTCTTCC AAGAGAAGGT 
TCTGTAGGCT CTACCAATGA TTATGTCGGT CAAAGCTATT CATATTCTTC AATCTTGAAT 
AAATCAGAAA CAGGATATGT GGGATTAGTA AATCAAGCAA TGACTTGCTA TTTGAACAGC 
CTTTTGCAAA CACTTTTCAT GACTCCTGAA TTCAGGAATG CACTGTATAA GTGGGAATTT 
GAGGAATCTG AAGAAGATCC TGTGACAAGC ATTCCATACC AGCTTCAGAG ACTCTTTGTT 
TTATTACAAA CCAGCAAAAA GAGAGCAATT GAAACGACTG ATGTTACAAG AAGCTTTGGA 
TGGGATAGTA GTGAGGCTTG GCAACAACAT GATGTACAAG AACTTTGTAG AGTCATGTTT 
GATGCATTGG AACAGAAATG GAAGCAAACA GAGCAGGCCG ATCTTATAAA TGAACTGTAT 
CAAGGAAAGC TGAAGGACTA CGTGAGATGT CTGGAATGTG GTTATGAGGG CTGGAGAATC 
GACACATATC TTGACATTCC ATTGGTCATC CGACCTTATG GTGCCAGCCA GGCATTTGCT 
AGTGTGGAAG AAGCATTACA AGCATTTATA CAGCCAGAAA TCCTGGATGG CCCCAACCAG 
TACTTTTGTG AGCAGTGTAA AAAGAAGTGT GATGCACGGA AGGGCCTTCG TTTTTTGCAT 
TTCCCATATC TGCTGACTCT CCAGTTGAAA AGGTTTGACT TTGATTACAC CACCATGCAC 
AGGATTAAAT TAAATGACCG GATGACTTTC CCAGAAGAGC TAGATATGAG TACATTTATT 
GATGTTGAAG ATGAGAATTC CTTGCTATAC GAACTCTTTT CTGTCATGGT TCATTCAGGA 
AGTGCAGCTG GTGGTCATTA TTATGCGTGC ATAAAATCAT TCAGTGATGA GCAGTGGTAC 
AGCTTCAATG ATCAGCATGT TAGTCGGATA ACCCAAGAGG ACATTAAGAA GACTCATGGC 
GGATCTTCAG GAAGTCGAGG ATATTATTCT AGTGCCTTTG CCAGCTCCAC AAATGCATAT 
ATGCTTATAT ATAGATTGAA GGATCCTTCA AGAAATGCAA AATTTTTAGA AGTGAAAGAT 
TACCCAGAGC ACATTAAGAA TTTGGTGCGA AAAGAGAAAG AACTGGAAGA GCAAGAAAAA 
AAGCAACGTG AAATTGAGCG AAATACCTGC AAGATAAAAT TGTTCTGCAT GCATCCTGTG 
AAACAAGTGA TGATGGAAAA TAAATTGGAG GTTCATAAAG ATAAGACCTT AAAAGAAGCA 
GTAGAAATAG CTTATAAGGT GATGGATTTA GAAGAAGCCA TTCCCCTGGA CTGCTGTCGT 
CTTGTTAAGT ATGATGAGTT TCATGATTAT CTAGAGAGGT CATATGAAGG AGAAGAGGAT 
ACTCCAATGG GACTTCTACT TGGTGGAGTC AAATCAACAT ACATGTTTGA TCTGCTCTTG 
GAAATCAAAA AACCTGATCA GGTTTTTCAG TCTTATAAAC CTGGTGAGGT GATGGTGAAG 
GTCCATGTGG TTGATATAAA GGCAGAGTCT GTTGCTCCTC CATTTAGTGT ACGAGCTTAT 
TTAAATCAAA CAGTCGCCGA GTTTAAGCAA CTTATTTCAA AGACTATACA GCTGCCTGCT 
GAAACGATGA GAATAGTTCT AGAACGCTGC TACAATGATT TGCGTCTTCT CAATGTGCCC 
AGTAAAACAC TGAAGGCTGA AGGCTTCTTT AGAAGTAACA AGGTATTTGT CGAAAGTTCT 
GAGTCTTTGG ATCACCAGAT GGCCTTTACA GATTCTCATT TGTGGAAATT GCTGGATCGA 
CATGCAAATA CAATCAGACT GTTTGTTTTA TTACCTGAAC AATCTCCAGG ATCTCATTCT 
AAAAGGACAG TGTACCAGAA AGCTGGTGGG GATTCTGGCA ACATGGATGA TGCTTGTGAA 
CGAGTTAAAG GACCTCTAGG TAGCCTAAAA TCTGTAGAGG CTATCTTAGA AGAAAGTACT 
GAAAAGCTTA AAAGCTTGTC TCTTCGACAG CAACAGGATG GAGATAATGG AGACAGTAGC 
AAGAGTACAG AGACAAGTGA TTTTGAAAAC ATTGAATCAC CTCTAAATGA GAGAGACTCT 
TCAGGATCAG TAGATACCAG AGAACTTGAA CAGCAGATTC CCACTTCTGA TCCAGAAAAT 
TTCCAGTCTG AAGAGCGATC CGACTCTGAT GTGAACAATG ACAGGAGTAC AAGCTCAGTG 
GACAGTGACA TTCTTAGTTC CAGTCACAGT AGTGACACTT TGTGCAATGC AGACAGTGCC 
CCGATCCCTT TGGCTAATGG ACTTGACTCT CATAGTATCA CAAGTAGTAG AAGATCTAAG 
GCAAATGAAG GAAAAAAGGA GACCTGGGAT ACAGCTGAAG AAGACTCTGG AACTGACAGT 
GAATATGATG AAAGTGGCAA GAGTAGGGGA GAGACACAGT ACATATATTT CAAAGCAGAA 
TCCTATCCTG CAGATGAAGG TTCTGGGGAA GGACATAAAT GGTTGATGGT GCATGTTGAT 
AAAAGAATTA CACTGGCTAC CTTCAAACAA CAGTTGGAAC CCTTTGTTGG AGCTCTGTCT 
TCTCACTTCA AAGTTTTTCG AGTATATGCC AGCAATCAAG AGTTTGAAAG CATCCGACTG 
AATGAAACAC TTTCATCATT TTCAGATGAC AACAAGATTA CAATTAGATT GGGGAGAGCA 
CTTAAGAAGG GAGAGTACAG AGTCAAAGTG TACCAGCTTT TAGTAAATGA GCAAGAGCCC 
TGTAGGTTTC TGCTTGATGC GGTTTTTGCT AAAGGCATGA CTGTTCGACA GTCTAAAGAA 
GAGCTCATCC CCCAGCTCAG GGAGCAGTGT GGGCTAGAAG TCAGCATTGA CAGGTTTCGT 
CTAAGGAAAA AAACATGGAA GAATCCTGGC ACAGTCTTTT TGGATTATCA TGTCTATGAA 
GAGGATATTA ATATTTCCAG CAACTGGGAG GTTTTCCTTG AAGTTCTTGA TGAAGTGGAG 
AGGATGAAAT CCATGTCACA ATTGGCTGTA CTGGCCAGAA GATGGAGACC AACAGAGCTG 
ATACTGGACC CCTTCCAAGA GATAGTGTTG GAAAGCAGTA GTGTTGATGA ACTGAGAGAG 
AAGCTCTGCG AAATCAGTGG GATACCTTTG GAAGATGTGG AATTTGCAAA GGGAAGAGGA 
ACATTTCCCT GTGACATGTC TGTCCTAGAA ATTCACCAGG ATTTGGATTG GAATCCCAAA 
GTTTCTACAT TGAATGTGTG GCCCCTTTAT ATCTGTGATG ATGGTGCGGT GATATTTTAT 
AGGGATAAAA CTGAAGAATT ATTGGAATTA ACAGATGAGC AGAGAAATGA ACTAATGAAA 
AAGGAAAGCA GCCGGCTCCA GAAAACTGGA CATCGTGTAA CCTACTCTCC TCGTAAAGAA 
AAGGCACTAA AAATTTATCT GGACGGATCA TCAAATAAAG ATTTGACTCA AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP47 ( XM_012552518.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012407972.1 Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa135894
Clone ID Related Accession (Same CDS sequence)	XM_012552518.1 , XM_012552518.2
Accession Version	XM_012552518.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003846892.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012552518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCGG GCGAAGAGAA CCAGCTAGTC CCGAAGGAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTTT TATGTATTAT ACAAGATACT ACTAATTCAA AGACAGTCAA TGAACGAATC 
ACTTTAAATT TACCAGCTTC TACTCCACTG AGGAAACTAT TTGAAGATGT TGCCAACAAA 
GTGGGCTATG TAAATGGAAC CTTTGATTTG GTATGGGGAA ATGGAATCAA TGCTACTGAC 
ATGCCTCCTT TGGATCACAC CAGTGAAAAA TCTCTTCTTG ACACTAACTT TGAACCTGGG 
AAGAAAAACT TTCTGCATTT GACAGATAAA GATGGTGAAC AGCCTCAGAT AGCAGAGGAT 
ACCATTGCAG CAGATGACAG TGTGCAAGAT AGGTTTATAG GCCCTCTTCC AAGAGAAGGT 
TCTGTAGGCT CTACCAATGA TTATGTCGGT CAAAGCTATT CATATTCTTC AATCTTGAAT 
AAATCAGAAA CAGGATATGT GGGATTAGTA AATCAAGCAA TGACTTGCTA TTTGAACAGC 
CTTTTGCAAA CACTTTTCAT GACTCCTGAA TTCAGGAATG CACTGTATAA GTGGGAATTT 
GAGGAATCTG AAGAAGATCC TGTGACAAGC ATTCCATACC AGCTTCAGAG ACTCTTTGTT 
TTATTACAAA CCAGCAAAAA GAGAGCAATT GAAACGACTG ATGTTACAAG AAGCTTTGGA 
TGGGATAGTA GTGAGGCTTG GCAACAACAT GATGTACAAG AACTTTGTAG AGTCATGTTT 
GATGCATTGG AACAGAAATG GAAGCAAACA GAGCAGGCCG ATCTTATAAA TGAACTGTAT 
CAAGGAAAGC TGAAGGACTA CGTGAGATGT CTGGAATGTG GTTATGAGGG CTGGAGAATC 
GACACATATC TTGACATTCC ATTGGTCATC CGACCTTATG GTGCCAGCCA GGCATTTGCT 
AGTGTGGAAG AAGCATTACA AGCATTTATA CAGCCAGAAA TCCTGGATGG CCCCAACCAG 
TACTTTTGTG AGCAGTGTAA AAAGAAGTGT GATGCACGGA AGGGCCTTCG TTTTTTGCAT 
TTCCCATATC TGCTGACTCT CCAGTTGAAA AGGTTTGACT TTGATTACAC CACCATGCAC 
AGGATTAAAT TAAATGACCG GATGACTTTC CCAGAAGAGC TAGATATGAG TACATTTATT 
GATGTTGAAG ATGAGAATTC CTTGCTATAC GAACTCTTTT CTGTCATGGT TCATTCAGGA 
AGTGCAGCTG GTGGTCATTA TTATGCGTGC ATAAAATCAT TCAGTGATGA GCAGTGGTAC 
AGCTTCAATG ATCAGCATGT TAGTCGGATA ACCCAAGAGG ACATTAAGAA GACTCATGGC 
GGATCTTCAG GAAGTCGAGG ATATTATTCT AGTGCCTTTG CCAGCTCCAC AAATGCATAT 
ATGCTTATAT ATAGATTGAA GGATCCTTCA AGAAATGCAA AATTTTTAGA AGTGAAAGAT 
TACCCAGAGC ACATTAAGAA TTTGGTGCGA AAAGAGAAAG AACTGGAAGA GCAAGAAAAA 
AAGCAACGTG AAATTGAGCG AAATACCTGC AAGATAAAAT TGTTCTGCAT GCATCCTGTG 
AAACAAGTGA TGATGGAAAA TAAATTGGAG GTTCATAAAG ATAAGACCTT AAAAGAAGCA 
GTAGAAATAG CTTATAAGGT GATGGATTTA GAAGAAGCCA TTCCCCTGGA CTGCTGTCGT 
CTTGTTAAGT ATGATGAGTT TCATGATTAT CTAGAGAGGT CATATGAAGG AGAAGAGGAT 
ACTCCAATGG GACTTCTACT TGGTGGAGTC AAATCAACAT ACATGTTTGA TCTGCTCTTG 
GAAATCAAAA AACCTGATCA GGTTTTTCAG TCTTATAAAC CTGGTGAGGT GATGGTGAAG 
GTCCATGTGG TTGATATAAA GGCAGAGTCT GTTGCTCCTC CATTTAGTGT ACGAGCTTAT 
TTAAATCAAA CAGTCGCCGA GTTTAAGCAA CTTATTTCAA AGACTATACA GCTGCCTGCT 
GAAACGATGA GAATAGTTCT AGAACGCTGC TACAATGATT TGCGTCTTCT CAATGTGCCC 
AGTAAAACAC TGAAGGCTGA AGGCTTCTTT AGAAGTAACA AGGTATTTGT CGAAAGTTCT 
GAGTCTTTGG ATCACCAGAT GGCCTTTACA GATTCTCATT TGTGGAAATT GCTGGATCGA 
CATGCAAATA CAATCAGACT GTTTGTTTTA TTACCTGAAC AATCTCCAGG ATCTCATTCT 
AAAAGGACAG TGTACCAGAA AGCTGGTGGG GATTCTGGCA ACATGGATGA TGCTTGTGAA 
CGAGTTAAAG GACCTCTAGG TAGCCTAAAA TCTGTAGAGG CTATCTTAGA AGAAAGTACT 
GAAAAGCTTA AAAGCTTGTC TCTTCGACAG CAACAGGATG GAGATAATGG AGACAGTAGC 
AAGAGTACAG AGACAAGTGA TTTTGAAAAC ATTGAATCAC CTCTAAATGA GAGAGACTCT 
TCAGGATCAG TAGATACCAG AGAACTTGAA CAGCAGATTC CCACTTCTGA TCCAGAAAAT 
TTCCAGTCTG AAGAGCGATC CGACTCTGAT GTGAACAATG ACAGGAGTAC AAGCTCAGTG 
GACAGTGACA TTCTTAGTTC CAGTCACAGT AGTGACACTT TGTGCAATGC AGACAGTGCC 
CCGATCCCTT TGGCTAATGG ACTTGACTCT CATAGTATCA CAAGTAGTAG AAGATCTAAG 
GCAAATGAAG GAAAAAAGGA GACCTGGGAT ACAGCTGAAG AAGACTCTGG AACTGACAGT 
GAATATGATG AAAGTGGCAA GAGTAGGGGA GAGACACAGT ACATATATTT CAAAGCAGAA 
TCCTATCCTG CAGATGAAGG TTCTGGGGAA GGACATAAAT GGTTGATGGT GCATGTTGAT 
AAAAGAATTA CACTGGCTAC CTTCAAACAA CAGTTGGAAC CCTTTGTTGG AGCTCTGTCT 
TCTCACTTCA AAGTTTTTCG AGTATATGCC AGCAATCAAG AGTTTGAAAG CATCCGACTG 
AATGAAACAC TTTCATCATT TTCAGATGAC AACAAGATTA CAATTAGATT GGGGAGAGCA 
CTTAAGAAGG GAGAGTACAG AGTCAAAGTG TACCAGCTTT TAGTAAATGA GCAAGAGCCC 
TGTAGGTTTC TGCTTGATGC GGTTTTTGCT AAAGGCATGA CTGTTCGACA GTCTAAAGAA 
GAGCTCATCC CCCAGCTCAG GGAGCAGTGT GGGCTAGAAG TCAGCATTGA CAGGTTTCGT 
CTAAGGAAAA AAACATGGAA GAATCCTGGC ACAGTCTTTT TGGATTATCA TGTCTATGAA 
GAGGATATTA ATATTTCCAG CAACTGGGAG GTTTTCCTTG AAGTTCTTGA TGAAGTGGAG 
AGGATGAAAT CCATGTCACA ATTGGCTGTA CTGGCCAGAA GATGGAGACC AACAGAGCTG 
ATACTGGACC CCTTCCAAGA GATAGTGTTG GAAAGCAGTA GTGTTGATGA ACTGAGAGAG 
AAGCTCTGCG AAATCAGTGG GATACCTTTG GAAGATGTGG AATTTGCAAA GGGAAGAGGA 
ACATTTCCCT GTGACATGTC TGTCCTAGAA ATTCACCAGG ATTTGGATTG GAATCCCAAA 
GTTTCTACAT TGAATGTGTG GCCCCTTTAT ATCTGTGATG ATGGTGCGGT GATATTTTAT 
AGGGATAAAA CTGAAGAATT ATTGGAATTA ACAGATGAGC AGAGAAATGA ACTAATGAAA 
AAGGAAAGCA GCCGGCTCCA GAAAACTGGA CATCGTGTAA CCTACTCTCC TCGTAAAGAA 
AAGGCACTAA AAATTTATCT GGACGGATCA TCAAATAAAG ATTTGACTCA AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012407972.1
CDS	327..4223
Translation	MVPGEENQLVPKEIENAAEEPRVLCIIQDTTNSKTVNERITLNLPASTPLRKLFEDVANKVGYVNGTFDLVWGNGINATDMPPLDHTSEKSLLDTNFEPGKKNFLHLTDKDGEQPQIAEDTIAADDSVQDRFIGPLPREGSVGSTNDYVGQSYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEESEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGASQAFASVEEALQAFIQPEILDGPNQYFCEQCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMSTFIDVEDENSLLYELFSVMVHSGSAAGGHYYACIKSFSDEQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPSRNAKFLEVKDYPEHIKNLVRKEKELEEQEKKQREIERNTCKIKLFCMHPVKQVMMENKLEVHKDKTLKEAVEIAYKVMDLEEAIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLEIKKPDQVFQSYKPGEVMVKVHVVDIKAESVAPPFSVRAYLNQTVAEFKQLISKTIQLPAETMRIVLERCYNDLRLLNVPSKTLKAEGFFRSNKVFVESSESLDHQMAFTDSHLWKLLDRHANTIRLFVLLPEQSPGSHSKRTVYQKAGGDSGNMDDACERVKGPLGSLKSVEAILEESTEKLKSLSLRQQQDGDNGDSSKSTETSDFENIESPLNERDSSGSVDTRELEQQIPTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSAPIPLANGLDSHSITSSRRSKANEGKKETWDTAEEDSGTDSEYDESGKSRGETQYIYFKAESYPADEGSGEGHKWLMVHVDKRITLATFKQQLEPFVGALSSHFKVFRVYASNQEFESIRLNETLSSFSDDNKITIRLGRALKKGEYRVKVYQLLVNEQEPCRFLLDAVFAKGMTVRQSKEELIPQLREQCGLEVSIDRFRLRKKTWKNPGTVFLDYHVYEEDINISSNWEVFLEVLDEVERMKSMSQLAVLARRWRPTELILDPFQEIVLESSSVDELREKLCEISGIPLEDVEFAKGRGTFPCDMSVLEIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDKTEELLELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGSSNKDLTQD

Target ORF information:

RefSeq Version	XM_012552518.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012552518.2

ORF Insert Sequence:

ATGGTCCCGG GCGAAGAGAA CCAGCTAGTC CCGAAGGAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTTT TATGTATTAT ACAAGATACT ACTAATTCAA AGACAGTCAA TGAACGAATC 
ACTTTAAATT TACCAGCTTC TACTCCACTG AGGAAACTAT TTGAAGATGT TGCCAACAAA 
GTGGGCTATG TAAATGGAAC CTTTGATTTG GTATGGGGAA ATGGAATCAA TGCTACTGAC 
ATGCCTCCTT TGGATCACAC CAGTGAAAAA TCTCTTCTTG ACACTAACTT TGAACCTGGG 
AAGAAAAACT TTCTGCATTT GACAGATAAA GATGGTGAAC AGCCTCAGAT AGCAGAGGAT 
ACCATTGCAG CAGATGACAG TGTGCAAGAT AGGTTTATAG GCCCTCTTCC AAGAGAAGGT 
TCTGTAGGCT CTACCAATGA TTATGTCGGT CAAAGCTATT CATATTCTTC AATCTTGAAT 
AAATCAGAAA CAGGATATGT GGGATTAGTA AATCAAGCAA TGACTTGCTA TTTGAACAGC 
CTTTTGCAAA CACTTTTCAT GACTCCTGAA TTCAGGAATG CACTGTATAA GTGGGAATTT 
GAGGAATCTG AAGAAGATCC TGTGACAAGC ATTCCATACC AGCTTCAGAG ACTCTTTGTT 
TTATTACAAA CCAGCAAAAA GAGAGCAATT GAAACGACTG ATGTTACAAG AAGCTTTGGA 
TGGGATAGTA GTGAGGCTTG GCAACAACAT GATGTACAAG AACTTTGTAG AGTCATGTTT 
GATGCATTGG AACAGAAATG GAAGCAAACA GAGCAGGCCG ATCTTATAAA TGAACTGTAT 
CAAGGAAAGC TGAAGGACTA CGTGAGATGT CTGGAATGTG GTTATGAGGG CTGGAGAATC 
GACACATATC TTGACATTCC ATTGGTCATC CGACCTTATG GTGCCAGCCA GGCATTTGCT 
AGTGTGGAAG AAGCATTACA AGCATTTATA CAGCCAGAAA TCCTGGATGG CCCCAACCAG 
TACTTTTGTG AGCAGTGTAA AAAGAAGTGT GATGCACGGA AGGGCCTTCG TTTTTTGCAT 
TTCCCATATC TGCTGACTCT CCAGTTGAAA AGGTTTGACT TTGATTACAC CACCATGCAC 
AGGATTAAAT TAAATGACCG GATGACTTTC CCAGAAGAGC TAGATATGAG TACATTTATT 
GATGTTGAAG ATGAGAATTC CTTGCTATAC GAACTCTTTT CTGTCATGGT TCATTCAGGA 
AGTGCAGCTG GTGGTCATTA TTATGCGTGC ATAAAATCAT TCAGTGATGA GCAGTGGTAC 
AGCTTCAATG ATCAGCATGT TAGTCGGATA ACCCAAGAGG ACATTAAGAA GACTCATGGC 
GGATCTTCAG GAAGTCGAGG ATATTATTCT AGTGCCTTTG CCAGCTCCAC AAATGCATAT 
ATGCTTATAT ATAGATTGAA GGATCCTTCA AGAAATGCAA AATTTTTAGA AGTGAAAGAT 
TACCCAGAGC ACATTAAGAA TTTGGTGCGA AAAGAGAAAG AACTGGAAGA GCAAGAAAAA 
AAGCAACGTG AAATTGAGCG AAATACCTGC AAGATAAAAT TGTTCTGCAT GCATCCTGTG 
AAACAAGTGA TGATGGAAAA TAAATTGGAG GTTCATAAAG ATAAGACCTT AAAAGAAGCA 
GTAGAAATAG CTTATAAGGT GATGGATTTA GAAGAAGCCA TTCCCCTGGA CTGCTGTCGT 
CTTGTTAAGT ATGATGAGTT TCATGATTAT CTAGAGAGGT CATATGAAGG AGAAGAGGAT 
ACTCCAATGG GACTTCTACT TGGTGGAGTC AAATCAACAT ACATGTTTGA TCTGCTCTTG 
GAAATCAAAA AACCTGATCA GGTTTTTCAG TCTTATAAAC CTGGTGAGGT GATGGTGAAG 
GTCCATGTGG TTGATATAAA GGCAGAGTCT GTTGCTCCTC CATTTAGTGT ACGAGCTTAT 
TTAAATCAAA CAGTCGCCGA GTTTAAGCAA CTTATTTCAA AGACTATACA GCTGCCTGCT 
GAAACGATGA GAATAGTTCT AGAACGCTGC TACAATGATT TGCGTCTTCT CAATGTGCCC 
AGTAAAACAC TGAAGGCTGA AGGCTTCTTT AGAAGTAACA AGGTATTTGT CGAAAGTTCT 
GAGTCTTTGG ATCACCAGAT GGCCTTTACA GATTCTCATT TGTGGAAATT GCTGGATCGA 
CATGCAAATA CAATCAGACT GTTTGTTTTA TTACCTGAAC AATCTCCAGG ATCTCATTCT 
AAAAGGACAG TGTACCAGAA AGCTGGTGGG GATTCTGGCA ACATGGATGA TGCTTGTGAA 
CGAGTTAAAG GACCTCTAGG TAGCCTAAAA TCTGTAGAGG CTATCTTAGA AGAAAGTACT 
GAAAAGCTTA AAAGCTTGTC TCTTCGACAG CAACAGGATG GAGATAATGG AGACAGTAGC 
AAGAGTACAG AGACAAGTGA TTTTGAAAAC ATTGAATCAC CTCTAAATGA GAGAGACTCT 
TCAGGATCAG TAGATACCAG AGAACTTGAA CAGCAGATTC CCACTTCTGA TCCAGAAAAT 
TTCCAGTCTG AAGAGCGATC CGACTCTGAT GTGAACAATG ACAGGAGTAC AAGCTCAGTG 
GACAGTGACA TTCTTAGTTC CAGTCACAGT AGTGACACTT TGTGCAATGC AGACAGTGCC 
CCGATCCCTT TGGCTAATGG ACTTGACTCT CATAGTATCA CAAGTAGTAG AAGATCTAAG 
GCAAATGAAG GAAAAAAGGA GACCTGGGAT ACAGCTGAAG AAGACTCTGG AACTGACAGT 
GAATATGATG AAAGTGGCAA GAGTAGGGGA GAGACACAGT ACATATATTT CAAAGCAGAA 
TCCTATCCTG CAGATGAAGG TTCTGGGGAA GGACATAAAT GGTTGATGGT GCATGTTGAT 
AAAAGAATTA CACTGGCTAC CTTCAAACAA CAGTTGGAAC CCTTTGTTGG AGCTCTGTCT 
TCTCACTTCA AAGTTTTTCG AGTATATGCC AGCAATCAAG AGTTTGAAAG CATCCGACTG 
AATGAAACAC TTTCATCATT TTCAGATGAC AACAAGATTA CAATTAGATT GGGGAGAGCA 
CTTAAGAAGG GAGAGTACAG AGTCAAAGTG TACCAGCTTT TAGTAAATGA GCAAGAGCCC 
TGTAGGTTTC TGCTTGATGC GGTTTTTGCT AAAGGCATGA CTGTTCGACA GTCTAAAGAA 
GAGCTCATCC CCCAGCTCAG GGAGCAGTGT GGGCTAGAAG TCAGCATTGA CAGGTTTCGT 
CTAAGGAAAA AAACATGGAA GAATCCTGGC ACAGTCTTTT TGGATTATCA TGTCTATGAA 
GAGGATATTA ATATTTCCAG CAACTGGGAG GTTTTCCTTG AAGTTCTTGA TGAAGTGGAG 
AGGATGAAAT CCATGTCACA ATTGGCTGTA CTGGCCAGAA GATGGAGACC AACAGAGCTG 
ATACTGGACC CCTTCCAAGA GATAGTGTTG GAAAGCAGTA GTGTTGATGA ACTGAGAGAG 
AAGCTCTGCG AAATCAGTGG GATACCTTTG GAAGATGTGG AATTTGCAAA GGGAAGAGGA 
ACATTTCCCT GTGACATGTC TGTCCTAGAA ATTCACCAGG ATTTGGATTG GAATCCCAAA 
GTTTCTACAT TGAATGTGTG GCCCCTTTAT ATCTGTGATG ATGGTGCGGT GATATTTTAT 
AGGGATAAAA CTGAAGAATT ATTGGAATTA ACAGATGAGC AGAGAAATGA ACTAATGAAA 
AAGGAAAGCA GCCGGCTCCA GAAAACTGGA CATCGTGTAA CCTACTCTCC TCGTAAAGAA 
AAGGCACTAA AAATTTATCT GGACGGATCA TCAAATAAAG ATTTGACTCA AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP47 ( XM_023506281.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023362049.1 Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X3, mRNA.

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CloneID	OSa157461
Clone ID Related Accession (Same CDS sequence)	XM_023506281.1 , XM_031944164.1
Accession Version	XM_023506281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003846892.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCGG GCGAAGAGAA CCAGCTAGTC CCGAAGGAGG ATGTGTTTTG GAGATGCAGA 
CAGAGTATCT TTGATGAAAT GAAGAAGAAA TTTGCACAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTTT TATGTATTAT ACAAGATACT ACTAATTCAA AGACAGTCAA TGAACGAATC 
ACTTTAAATT TACCAGCTTC TACTCCACTG AGGAAACTAT TTGAAGATGT TGCCAACAAA 
GTGGGCTATG TAAATGGAAC CTTTGATTTG GTATGGGGAA ATGGAATCAA TGCTACTGAC 
ATGCCTCCTT TGGATCACAC CAGTGAAAAA TCTCTTCTTG ACACTAACTT TGAACCTGGG 
AAGAAAAACT TTCTGCATTT GACAGATAAA GATGGTGAAC AGCCTCAGAT AGCAGAGGAT 
ACCATTGCAG CAGATGACAG TGTGCAAGAT AGGTTTATAG GCCCTCTTCC AAGAGAAGGT 
TCTGTAGGCT CTACCAATGA TTATGTCGGT CAAAGCTATT CATATTCTTC AATCTTGAAT 
AAATCAGAAA CAGGATATGT GGGATTAGTA AATCAAGCAA TGACTTGCTA TTTGAACAGC 
CTTTTGCAAA CACTTTTCAT GACTCCTGAA TTCAGGAATG CACTGTATAA GTGGGAATTT 
GAGGAATCTG AAGAAGATCC TGTGACAAGC ATTCCATACC AGCTTCAGAG ACTCTTTGTT 
TTATTACAAA CCAGCAAAAA GAGAGCAATT GAAACGACTG ATGTTACAAG AAGCTTTGGA 
TGGGATAGTA GTGAGGCTTG GCAACAACAT GATGTACAAG AACTTTGTAG AGTCATGTTT 
GATGCATTGG AACAGAAATG GAAGCAAACA GAGCAGGCCG ATCTTATAAA TGAACTGTAT 
CAAGGAAAGC TGAAGGACTA CGTGAGATGT CTGGAATGTG GTTATGAGGG CTGGAGAATC 
GACACATATC TTGACATTCC ATTGGTCATC CGACCTTATG GTGCCAGCCA GGCATTTGCT 
AGTGTGGAAG AAGCATTACA AGCATTTATA CAGCCAGAAA TCCTGGATGG CCCCAACCAG 
TACTTTTGTG AGCAGTGTAA AAAGAAGTGT GATGCACGGA AGGGCCTTCG TTTTTTGCAT 
TTCCCATATC TGCTGACTCT CCAGTTGAAA AGGTTTGACT TTGATTACAC CACCATGCAC 
AGGATTAAAT TAAATGACCG GATGACTTTC CCAGAAGAGC TAGATATGAG TACATTTATT 
GATGTTGAAG ATGAGAAGTC TCCTCAGACT GAGAGTTGTA CCGACAGTGG AGCTGAAAAT 
GAAGGCAGTT GTCACAGTGA TCAGATGAGC AACGATTTCT CAAATGATGA TGGGGTCGAT 
GAAGGAATCT GCCTTGAAAG TAATAGTGGA ACTGAAAAGA TCACAAAACC TGGTCTTGAA 
AAGAATTCCT TGCTATACGA ACTCTTTTCT GTCATGGTTC ATTCAGGAAG TGCAGCTGGT 
GGTCATTATT ATGCGTGCAT AAAATCATTC AGTGATGAGC AGTGGTACAG CTTCAATGAT 
CAGCATGTTA GTCGGATAAC CCAAGAGGAC ATTAAGAAGA CTCATGGCGG ATCTTCAGGA 
AGTCGAGGAT ATTATTCTAG TGCCTTTGCC AGCTCCACAA ATGCATATAT GCTTATATAT 
AGATTGAAGG ATCCTTCAAG AAATGCAAAA TTTTTAGAAG TGAAAGATTA CCCAGAGCAC 
ATTAAGAATT TGGTGCGAAA AGAGAAAGAA CTGGAAGAGC AAGAAAAAAA GCAACGTGAA 
ATTGAGCGAA ATACCTGCAA GATAAAATTG TTCTGCATGC ATCCTGTGAA ACAAGTGATG 
ATGGAAAATA AATTGGAGGT TCATAAAGAT AAGACCTTAA AAGAAGCAGT AGAAATAGCT 
TATAAGGTGA TGGATTTAGA AGAAGCCATT CCCCTGGACT GCTGTCGTCT TGTTAAGTAT 
GATGAGTTTC ATGATTATCT AGAGAGGTCA TATGAAGGAG AAGAGGATAC TCCAATGGGA 
CTTCTACTTG GTGGAGTCAA ATCAACATAC ATGTTTGATC TGCTCTTGGA AATCAAAAAA 
CCTGATCAGG TTTTTCAGTC TTATAAACCT GGTGAGGTGA TGGTGAAGGT CCATGTGGTT 
GATATAAAGG CAGAGTCTGT TGCTCCTCCA TTTAGTGTAC GAGCTTATTT AAATCAAACA 
GTCGCCGAGT TTAAGCAACT TATTTCAAAG ACTATACAGC TGCCTGCTGA AACGATGAGA 
ATAGTTCTAG AACGCTGCTA CAATGATTTG CGTCTTCTCA ATGTGCCCAG TAAAACACTG 
AAGGCTGAAG GCTTCTTTAG AAGTAACAAG GTATTTGTCG AAAGTTCTGA GTCTTTGGAT 
CACCAGATGG CCTTTACAGA TTCTCATTTG TGGAAATTGC TGGATCGACA TGCAAATACA 
ATCAGACTGT TTGTTTTATT ACCTGAACAA TCTCCAGGAT CTCATTCTAA AAGGACAGTG 
TACCAGAAAG CTGGTGGGGA TTCTGGCAAC ATGGATGATG CTTGTGAACG AGTTAAAGGA 
CCTCTAGGTA GCCTAAAATC TGTAGAGGCT ATCTTAGAAG AAAGTACTGA AAAGCTTAAA 
AGCTTGTCTC TTCGACAGCA ACAGGATGGA GATAATGGAG ACAGTAGCAA GAGTACAGAG 
ACAAGTGATT TTGAAAACAT TGAATCACCT CTAAATGAGA GAGACTCTTC AGGATCAGTA 
GATACCAGAG AACTTGAACA GCAGATTCCC ACTTCTGATC CAGAAAATTT CCAGTCTGAA 
GAGCGATCCG ACTCTGATGT GAACAATGAC AGGAGTACAA GCTCAGTGGA CAGTGACATT 
CTTAGTTCCA GTCACAGTAG TGACACTTTG TGCAATGCAG ACAGTGCCCC GATCCCTTTG 
GCTAATGGAC TTGACTCTCA TAGTATCACA AGTAGTAGAA GATCTAAGGC AAATGAAGGA 
AAAAAGGAGA CCTGGGATAC AGCTGAAGAA GACTCTGGAA CTGACAGTGA ATATGATGAA 
AGTGGCAAGA GTAGGGGAGA GACACAGTAC ATATATTTCA AAGCAGAATC CTATCCTGCA 
GATGAAGGTT CTGGGGAAGG ACATAAATGG TTGATGGTGC ATGTTGATAA AAGAATTACA 
CTGGCTACCT TCAAACAACA GTTGGAACCC TTTGTTGGAG CTCTGTCTTC TCACTTCAAA 
GTTTTTCGAG TATATGCCAG CAATCAAGAG TTTGAAAGCA TCCGACTGAA TGAAACACTT 
TCATCATTTT CAGATGACAA CAAGATTACA ATTAGATTGG GGAGAGCACT TAAGAAGGGA 
GAGTACAGAG TCAAAGTGTA CCAGCTTTTA GTAAATGAGC AAGAGCCCTG TAGGTTTCTG 
CTTGATGCGG TTTTTGCTAA AGGCATGACT GTTCGACAGT CTAAAGAAGA GCTCATCCCC 
CAGCTCAGGG AGCAGTGTGG GCTAGAAGTC AGCATTGACA GGTTTCGTCT AAGGAAAAAA 
ACATGGAAGA ATCCTGGCAC AGTCTTTTTG GATTATCATG TCTATGAAGA GGATATTAAT 
ATTTCCAGCA ACTGGGAGGT TTTCCTTGAA GTTCTTGATG AAGTGGAGAG GATGAAATCC 
ATGTCACAAT TGGCTGTACT GGCCAGAAGA TGGAGACCAA CAGAGCTGAT ACTGGACCCC 
TTCCAAGAGA TAGTGTTGGA AAGCAGTAGT GTTGATGAAC TGAGAGAGAA GCTCTGCGAA 
ATCAGTGGGA TACCTTTGGA AGATGTGGAA TTTGCAAAGG GAAGAGGAAC ATTTCCCTGT 
GACATGTCTG TCCTAGAAAT TCACCAGGAT TTGGATTGGA ATCCCAAAGT TTCTACATTG 
AATGTGTGGC CCCTTTATAT CTGTGATGAT GGTGCGGTGA TATTTTATAG GGATAAAACT 
GAAGAATTAT TGGAATTAAC AGATGAGCAG AGAAATGAAC TAATGAAAAA GGAAAGCAGC 
CGGCTCCAGA AAACTGGACA TCGTGTAACC TACTCTCCTC GTAAAGAAAA GGCACTAAAA 
ATTTATCTGG ACGGATCATC AAATAAAGAT TTGACTCAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023362049.1
CDS	329..4453
Translation	MVPGEENQLVPKEDVFWRCRQSIFDEMKKKFAQIENAAEEPRVLCIIQDTTNSKTVNERITLNLPASTPLRKLFEDVANKVGYVNGTFDLVWGNGINATDMPPLDHTSEKSLLDTNFEPGKKNFLHLTDKDGEQPQIAEDTIAADDSVQDRFIGPLPREGSVGSTNDYVGQSYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEESEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGASQAFASVEEALQAFIQPEILDGPNQYFCEQCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMSTFIDVEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSNDDGVDEGICLESNSGTEKITKPGLEKNSLLYELFSVMVHSGSAAGGHYYACIKSFSDEQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPSRNAKFLEVKDYPEHIKNLVRKEKELEEQEKKQREIERNTCKIKLFCMHPVKQVMMENKLEVHKDKTLKEAVEIAYKVMDLEEAIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLEIKKPDQVFQSYKPGEVMVKVHVVDIKAESVAPPFSVRAYLNQTVAEFKQLISKTIQLPAETMRIVLERCYNDLRLLNVPSKTLKAEGFFRSNKVFVESSESLDHQMAFTDSHLWKLLDRHANTIRLFVLLPEQSPGSHSKRTVYQKAGGDSGNMDDACERVKGPLGSLKSVEAILEESTEKLKSLSLRQQQDGDNGDSSKSTETSDFENIESPLNERDSSGSVDTRELEQQIPTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSAPIPLANGLDSHSITSSRRSKANEGKKETWDTAEEDSGTDSEYDESGKSRGETQYIYFKAESYPADEGSGEGHKWLMVHVDKRITLATFKQQLEPFVGALSSHFKVFRVYASNQEFESIRLNETLSSFSDDNKITIRLGRALKKGEYRVKVYQLLVNEQEPCRFLLDAVFAKGMTVRQSKEELIPQLREQCGLEVSIDRFRLRKKTWKNPGTVFLDYHVYEEDINISSNWEVFLEVLDEVERMKSMSQLAVLARRWRPTELILDPFQEIVLESSSVDELREKLCEISGIPLEDVEFAKGRGTFPCDMSVLEIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDKTEELLELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGSSNKDLTQD

Target ORF information:

RefSeq Version	XM_023506281.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023506281.1

ORF Insert Sequence:

ATGGTCCCGG GCGAAGAGAA CCAGCTAGTC CCGAAGGAGG ATGTGTTTTG GAGATGCAGA 
CAGAGTATCT TTGATGAAAT GAAGAAGAAA TTTGCACAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTTT TATGTATTAT ACAAGATACT ACTAATTCAA AGACAGTCAA TGAACGAATC 
ACTTTAAATT TACCAGCTTC TACTCCACTG AGGAAACTAT TTGAAGATGT TGCCAACAAA 
GTGGGCTATG TAAATGGAAC CTTTGATTTG GTATGGGGAA ATGGAATCAA TGCTACTGAC 
ATGCCTCCTT TGGATCACAC CAGTGAAAAA TCTCTTCTTG ACACTAACTT TGAACCTGGG 
AAGAAAAACT TTCTGCATTT GACAGATAAA GATGGTGAAC AGCCTCAGAT AGCAGAGGAT 
ACCATTGCAG CAGATGACAG TGTGCAAGAT AGGTTTATAG GCCCTCTTCC AAGAGAAGGT 
TCTGTAGGCT CTACCAATGA TTATGTCGGT CAAAGCTATT CATATTCTTC AATCTTGAAT 
AAATCAGAAA CAGGATATGT GGGATTAGTA AATCAAGCAA TGACTTGCTA TTTGAACAGC 
CTTTTGCAAA CACTTTTCAT GACTCCTGAA TTCAGGAATG CACTGTATAA GTGGGAATTT 
GAGGAATCTG AAGAAGATCC TGTGACAAGC ATTCCATACC AGCTTCAGAG ACTCTTTGTT 
TTATTACAAA CCAGCAAAAA GAGAGCAATT GAAACGACTG ATGTTACAAG AAGCTTTGGA 
TGGGATAGTA GTGAGGCTTG GCAACAACAT GATGTACAAG AACTTTGTAG AGTCATGTTT 
GATGCATTGG AACAGAAATG GAAGCAAACA GAGCAGGCCG ATCTTATAAA TGAACTGTAT 
CAAGGAAAGC TGAAGGACTA CGTGAGATGT CTGGAATGTG GTTATGAGGG CTGGAGAATC 
GACACATATC TTGACATTCC ATTGGTCATC CGACCTTATG GTGCCAGCCA GGCATTTGCT 
AGTGTGGAAG AAGCATTACA AGCATTTATA CAGCCAGAAA TCCTGGATGG CCCCAACCAG 
TACTTTTGTG AGCAGTGTAA AAAGAAGTGT GATGCACGGA AGGGCCTTCG TTTTTTGCAT 
TTCCCATATC TGCTGACTCT CCAGTTGAAA AGGTTTGACT TTGATTACAC CACCATGCAC 
AGGATTAAAT TAAATGACCG GATGACTTTC CCAGAAGAGC TAGATATGAG TACATTTATT 
GATGTTGAAG ATGAGAAGTC TCCTCAGACT GAGAGTTGTA CCGACAGTGG AGCTGAAAAT 
GAAGGCAGTT GTCACAGTGA TCAGATGAGC AACGATTTCT CAAATGATGA TGGGGTCGAT 
GAAGGAATCT GCCTTGAAAG TAATAGTGGA ACTGAAAAGA TCACAAAACC TGGTCTTGAA 
AAGAATTCCT TGCTATACGA ACTCTTTTCT GTCATGGTTC ATTCAGGAAG TGCAGCTGGT 
GGTCATTATT ATGCGTGCAT AAAATCATTC AGTGATGAGC AGTGGTACAG CTTCAATGAT 
CAGCATGTTA GTCGGATAAC CCAAGAGGAC ATTAAGAAGA CTCATGGCGG ATCTTCAGGA 
AGTCGAGGAT ATTATTCTAG TGCCTTTGCC AGCTCCACAA ATGCATATAT GCTTATATAT 
AGATTGAAGG ATCCTTCAAG AAATGCAAAA TTTTTAGAAG TGAAAGATTA CCCAGAGCAC 
ATTAAGAATT TGGTGCGAAA AGAGAAAGAA CTGGAAGAGC AAGAAAAAAA GCAACGTGAA 
ATTGAGCGAA ATACCTGCAA GATAAAATTG TTCTGCATGC ATCCTGTGAA ACAAGTGATG 
ATGGAAAATA AATTGGAGGT TCATAAAGAT AAGACCTTAA AAGAAGCAGT AGAAATAGCT 
TATAAGGTGA TGGATTTAGA AGAAGCCATT CCCCTGGACT GCTGTCGTCT TGTTAAGTAT 
GATGAGTTTC ATGATTATCT AGAGAGGTCA TATGAAGGAG AAGAGGATAC TCCAATGGGA 
CTTCTACTTG GTGGAGTCAA ATCAACATAC ATGTTTGATC TGCTCTTGGA AATCAAAAAA 
CCTGATCAGG TTTTTCAGTC TTATAAACCT GGTGAGGTGA TGGTGAAGGT CCATGTGGTT 
GATATAAAGG CAGAGTCTGT TGCTCCTCCA TTTAGTGTAC GAGCTTATTT AAATCAAACA 
GTCGCCGAGT TTAAGCAACT TATTTCAAAG ACTATACAGC TGCCTGCTGA AACGATGAGA 
ATAGTTCTAG AACGCTGCTA CAATGATTTG CGTCTTCTCA ATGTGCCCAG TAAAACACTG 
AAGGCTGAAG GCTTCTTTAG AAGTAACAAG GTATTTGTCG AAAGTTCTGA GTCTTTGGAT 
CACCAGATGG CCTTTACAGA TTCTCATTTG TGGAAATTGC TGGATCGACA TGCAAATACA 
ATCAGACTGT TTGTTTTATT ACCTGAACAA TCTCCAGGAT CTCATTCTAA AAGGACAGTG 
TACCAGAAAG CTGGTGGGGA TTCTGGCAAC ATGGATGATG CTTGTGAACG AGTTAAAGGA 
CCTCTAGGTA GCCTAAAATC TGTAGAGGCT ATCTTAGAAG AAAGTACTGA AAAGCTTAAA 
AGCTTGTCTC TTCGACAGCA ACAGGATGGA GATAATGGAG ACAGTAGCAA GAGTACAGAG 
ACAAGTGATT TTGAAAACAT TGAATCACCT CTAAATGAGA GAGACTCTTC AGGATCAGTA 
GATACCAGAG AACTTGAACA GCAGATTCCC ACTTCTGATC CAGAAAATTT CCAGTCTGAA 
GAGCGATCCG ACTCTGATGT GAACAATGAC AGGAGTACAA GCTCAGTGGA CAGTGACATT 
CTTAGTTCCA GTCACAGTAG TGACACTTTG TGCAATGCAG ACAGTGCCCC GATCCCTTTG 
GCTAATGGAC TTGACTCTCA TAGTATCACA AGTAGTAGAA GATCTAAGGC AAATGAAGGA 
AAAAAGGAGA CCTGGGATAC AGCTGAAGAA GACTCTGGAA CTGACAGTGA ATATGATGAA 
AGTGGCAAGA GTAGGGGAGA GACACAGTAC ATATATTTCA AAGCAGAATC CTATCCTGCA 
GATGAAGGTT CTGGGGAAGG ACATAAATGG TTGATGGTGC ATGTTGATAA AAGAATTACA 
CTGGCTACCT TCAAACAACA GTTGGAACCC TTTGTTGGAG CTCTGTCTTC TCACTTCAAA 
GTTTTTCGAG TATATGCCAG CAATCAAGAG TTTGAAAGCA TCCGACTGAA TGAAACACTT 
TCATCATTTT CAGATGACAA CAAGATTACA ATTAGATTGG GGAGAGCACT TAAGAAGGGA 
GAGTACAGAG TCAAAGTGTA CCAGCTTTTA GTAAATGAGC AAGAGCCCTG TAGGTTTCTG 
CTTGATGCGG TTTTTGCTAA AGGCATGACT GTTCGACAGT CTAAAGAAGA GCTCATCCCC 
CAGCTCAGGG AGCAGTGTGG GCTAGAAGTC AGCATTGACA GGTTTCGTCT AAGGAAAAAA 
ACATGGAAGA ATCCTGGCAC AGTCTTTTTG GATTATCATG TCTATGAAGA GGATATTAAT 
ATTTCCAGCA ACTGGGAGGT TTTCCTTGAA GTTCTTGATG AAGTGGAGAG GATGAAATCC 
ATGTCACAAT TGGCTGTACT GGCCAGAAGA TGGAGACCAA CAGAGCTGAT ACTGGACCCC 
TTCCAAGAGA TAGTGTTGGA AAGCAGTAGT GTTGATGAAC TGAGAGAGAA GCTCTGCGAA 
ATCAGTGGGA TACCTTTGGA AGATGTGGAA TTTGCAAAGG GAAGAGGAAC ATTTCCCTGT 
GACATGTCTG TCCTAGAAAT TCACCAGGAT TTGGATTGGA ATCCCAAAGT TTCTACATTG 
AATGTGTGGC CCCTTTATAT CTGTGATGAT GGTGCGGTGA TATTTTATAG GGATAAAACT 
GAAGAATTAT TGGAATTAAC AGATGAGCAG AGAAATGAAC TAATGAAAAA GGAAAGCAGC 
CGGCTCCAGA AAACTGGACA TCGTGTAACC TACTCTCCTC GTAAAGAAAA GGCACTAAAA 
ATTTATCTGG ACGGATCATC AAATAAAGAT TTGACTCAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP47 ( XM_031944164.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031800024.1 Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X1, mRNA.

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CloneID	OSa157461
Clone ID Related Accession (Same CDS sequence)	XM_023506281.1 , XM_031944164.1
Accession Version	XM_031944164.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045431.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCGG GCGAAGAGAA CCAGCTAGTC CCGAAGGAGG ATGTGTTTTG GAGATGCAGA 
CAGAGTATCT TTGATGAAAT GAAGAAGAAA TTTGCACAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTTT TATGTATTAT ACAAGATACT ACTAATTCAA AGACAGTCAA TGAACGAATC 
ACTTTAAATT TACCAGCTTC TACTCCACTG AGGAAACTAT TTGAAGATGT TGCCAACAAA 
GTGGGCTATG TAAATGGAAC CTTTGATTTG GTATGGGGAA ATGGAATCAA TGCTACTGAC 
ATGCCTCCTT TGGATCACAC CAGTGAAAAA TCTCTTCTTG ACACTAACTT TGAACCTGGG 
AAGAAAAACT TTCTGCATTT GACAGATAAA GATGGTGAAC AGCCTCAGAT AGCAGAGGAT 
ACCATTGCAG CAGATGACAG TGTGCAAGAT AGGTTTATAG GCCCTCTTCC AAGAGAAGGT 
TCTGTAGGCT CTACCAATGA TTATGTCGGT CAAAGCTATT CATATTCTTC AATCTTGAAT 
AAATCAGAAA CAGGATATGT GGGATTAGTA AATCAAGCAA TGACTTGCTA TTTGAACAGC 
CTTTTGCAAA CACTTTTCAT GACTCCTGAA TTCAGGAATG CACTGTATAA GTGGGAATTT 
GAGGAATCTG AAGAAGATCC TGTGACAAGC ATTCCATACC AGCTTCAGAG ACTCTTTGTT 
TTATTACAAA CCAGCAAAAA GAGAGCAATT GAAACGACTG ATGTTACAAG AAGCTTTGGA 
TGGGATAGTA GTGAGGCTTG GCAACAACAT GATGTACAAG AACTTTGTAG AGTCATGTTT 
GATGCATTGG AACAGAAATG GAAGCAAACA GAGCAGGCCG ATCTTATAAA TGAACTGTAT 
CAAGGAAAGC TGAAGGACTA CGTGAGATGT CTGGAATGTG GTTATGAGGG CTGGAGAATC 
GACACATATC TTGACATTCC ATTGGTCATC CGACCTTATG GTGCCAGCCA GGCATTTGCT 
AGTGTGGAAG AAGCATTACA AGCATTTATA CAGCCAGAAA TCCTGGATGG CCCCAACCAG 
TACTTTTGTG AGCAGTGTAA AAAGAAGTGT GATGCACGGA AGGGCCTTCG TTTTTTGCAT 
TTCCCATATC TGCTGACTCT CCAGTTGAAA AGGTTTGACT TTGATTACAC CACCATGCAC 
AGGATTAAAT TAAATGACCG GATGACTTTC CCAGAAGAGC TAGATATGAG TACATTTATT 
GATGTTGAAG ATGAGAAGTC TCCTCAGACT GAGAGTTGTA CCGACAGTGG AGCTGAAAAT 
GAAGGCAGTT GTCACAGTGA TCAGATGAGC AACGATTTCT CAAATGATGA TGGGGTCGAT 
GAAGGAATCT GCCTTGAAAG TAATAGTGGA ACTGAAAAGA TCACAAAACC TGGTCTTGAA 
AAGAATTCCT TGCTATACGA ACTCTTTTCT GTCATGGTTC ATTCAGGAAG TGCAGCTGGT 
GGTCATTATT ATGCGTGCAT AAAATCATTC AGTGATGAGC AGTGGTACAG CTTCAATGAT 
CAGCATGTTA GTCGGATAAC CCAAGAGGAC ATTAAGAAGA CTCATGGCGG ATCTTCAGGA 
AGTCGAGGAT ATTATTCTAG TGCCTTTGCC AGCTCCACAA ATGCATATAT GCTTATATAT 
AGATTGAAGG ATCCTTCAAG AAATGCAAAA TTTTTAGAAG TGAAAGATTA CCCAGAGCAC 
ATTAAGAATT TGGTGCGAAA AGAGAAAGAA CTGGAAGAGC AAGAAAAAAA GCAACGTGAA 
ATTGAGCGAA ATACCTGCAA GATAAAATTG TTCTGCATGC ATCCTGTGAA ACAAGTGATG 
ATGGAAAATA AATTGGAGGT TCATAAAGAT AAGACCTTAA AAGAAGCAGT AGAAATAGCT 
TATAAGGTGA TGGATTTAGA AGAAGCCATT CCCCTGGACT GCTGTCGTCT TGTTAAGTAT 
GATGAGTTTC ATGATTATCT AGAGAGGTCA TATGAAGGAG AAGAGGATAC TCCAATGGGA 
CTTCTACTTG GTGGAGTCAA ATCAACATAC ATGTTTGATC TGCTCTTGGA AATCAAAAAA 
CCTGATCAGG TTTTTCAGTC TTATAAACCT GGTGAGGTGA TGGTGAAGGT CCATGTGGTT 
GATATAAAGG CAGAGTCTGT TGCTCCTCCA TTTAGTGTAC GAGCTTATTT AAATCAAACA 
GTCGCCGAGT TTAAGCAACT TATTTCAAAG ACTATACAGC TGCCTGCTGA AACGATGAGA 
ATAGTTCTAG AACGCTGCTA CAATGATTTG CGTCTTCTCA ATGTGCCCAG TAAAACACTG 
AAGGCTGAAG GCTTCTTTAG AAGTAACAAG GTATTTGTCG AAAGTTCTGA GTCTTTGGAT 
CACCAGATGG CCTTTACAGA TTCTCATTTG TGGAAATTGC TGGATCGACA TGCAAATACA 
ATCAGACTGT TTGTTTTATT ACCTGAACAA TCTCCAGGAT CTCATTCTAA AAGGACAGTG 
TACCAGAAAG CTGGTGGGGA TTCTGGCAAC ATGGATGATG CTTGTGAACG AGTTAAAGGA 
CCTCTAGGTA GCCTAAAATC TGTAGAGGCT ATCTTAGAAG AAAGTACTGA AAAGCTTAAA 
AGCTTGTCTC TTCGACAGCA ACAGGATGGA GATAATGGAG ACAGTAGCAA GAGTACAGAG 
ACAAGTGATT TTGAAAACAT TGAATCACCT CTAAATGAGA GAGACTCTTC AGGATCAGTA 
GATACCAGAG AACTTGAACA GCAGATTCCC ACTTCTGATC CAGAAAATTT CCAGTCTGAA 
GAGCGATCCG ACTCTGATGT GAACAATGAC AGGAGTACAA GCTCAGTGGA CAGTGACATT 
CTTAGTTCCA GTCACAGTAG TGACACTTTG TGCAATGCAG ACAGTGCCCC GATCCCTTTG 
GCTAATGGAC TTGACTCTCA TAGTATCACA AGTAGTAGAA GATCTAAGGC AAATGAAGGA 
AAAAAGGAGA CCTGGGATAC AGCTGAAGAA GACTCTGGAA CTGACAGTGA ATATGATGAA 
AGTGGCAAGA GTAGGGGAGA GACACAGTAC ATATATTTCA AAGCAGAATC CTATCCTGCA 
GATGAAGGTT CTGGGGAAGG ACATAAATGG TTGATGGTGC ATGTTGATAA AAGAATTACA 
CTGGCTACCT TCAAACAACA GTTGGAACCC TTTGTTGGAG CTCTGTCTTC TCACTTCAAA 
GTTTTTCGAG TATATGCCAG CAATCAAGAG TTTGAAAGCA TCCGACTGAA TGAAACACTT 
TCATCATTTT CAGATGACAA CAAGATTACA ATTAGATTGG GGAGAGCACT TAAGAAGGGA 
GAGTACAGAG TCAAAGTGTA CCAGCTTTTA GTAAATGAGC AAGAGCCCTG TAGGTTTCTG 
CTTGATGCGG TTTTTGCTAA AGGCATGACT GTTCGACAGT CTAAAGAAGA GCTCATCCCC 
CAGCTCAGGG AGCAGTGTGG GCTAGAAGTC AGCATTGACA GGTTTCGTCT AAGGAAAAAA 
ACATGGAAGA ATCCTGGCAC AGTCTTTTTG GATTATCATG TCTATGAAGA GGATATTAAT 
ATTTCCAGCA ACTGGGAGGT TTTCCTTGAA GTTCTTGATG AAGTGGAGAG GATGAAATCC 
ATGTCACAAT TGGCTGTACT GGCCAGAAGA TGGAGACCAA CAGAGCTGAT ACTGGACCCC 
TTCCAAGAGA TAGTGTTGGA AAGCAGTAGT GTTGATGAAC TGAGAGAGAA GCTCTGCGAA 
ATCAGTGGGA TACCTTTGGA AGATGTGGAA TTTGCAAAGG GAAGAGGAAC ATTTCCCTGT 
GACATGTCTG TCCTAGAAAT TCACCAGGAT TTGGATTGGA ATCCCAAAGT TTCTACATTG 
AATGTGTGGC CCCTTTATAT CTGTGATGAT GGTGCGGTGA TATTTTATAG GGATAAAACT 
GAAGAATTAT TGGAATTAAC AGATGAGCAG AGAAATGAAC TAATGAAAAA GGAAAGCAGC 
CGGCTCCAGA AAACTGGACA TCGTGTAACC TACTCTCCTC GTAAAGAAAA GGCACTAAAA 
ATTTATCTGG ACGGATCATC AAATAAAGAT TTGACTCAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031800024.1
CDS	200..4324
Translation	MVPGEENQLVPKEDVFWRCRQSIFDEMKKKFAQIENAAEEPRVLCIIQDTTNSKTVNERITLNLPASTPLRKLFEDVANKVGYVNGTFDLVWGNGINATDMPPLDHTSEKSLLDTNFEPGKKNFLHLTDKDGEQPQIAEDTIAADDSVQDRFIGPLPREGSVGSTNDYVGQSYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEESEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGASQAFASVEEALQAFIQPEILDGPNQYFCEQCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMSTFIDVEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSNDDGVDEGICLESNSGTEKITKPGLEKNSLLYELFSVMVHSGSAAGGHYYACIKSFSDEQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPSRNAKFLEVKDYPEHIKNLVRKEKELEEQEKKQREIERNTCKIKLFCMHPVKQVMMENKLEVHKDKTLKEAVEIAYKVMDLEEAIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLEIKKPDQVFQSYKPGEVMVKVHVVDIKAESVAPPFSVRAYLNQTVAEFKQLISKTIQLPAETMRIVLERCYNDLRLLNVPSKTLKAEGFFRSNKVFVESSESLDHQMAFTDSHLWKLLDRHANTIRLFVLLPEQSPGSHSKRTVYQKAGGDSGNMDDACERVKGPLGSLKSVEAILEESTEKLKSLSLRQQQDGDNGDSSKSTETSDFENIESPLNERDSSGSVDTRELEQQIPTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSAPIPLANGLDSHSITSSRRSKANEGKKETWDTAEEDSGTDSEYDESGKSRGETQYIYFKAESYPADEGSGEGHKWLMVHVDKRITLATFKQQLEPFVGALSSHFKVFRVYASNQEFESIRLNETLSSFSDDNKITIRLGRALKKGEYRVKVYQLLVNEQEPCRFLLDAVFAKGMTVRQSKEELIPQLREQCGLEVSIDRFRLRKKTWKNPGTVFLDYHVYEEDINISSNWEVFLEVLDEVERMKSMSQLAVLARRWRPTELILDPFQEIVLESSSVDELREKLCEISGIPLEDVEFAKGRGTFPCDMSVLEIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDKTEELLELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGSSNKDLTQD

Target ORF information:

RefSeq Version	XM_031944164.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031944164.1

ORF Insert Sequence:

ATGGTCCCGG GCGAAGAGAA CCAGCTAGTC CCGAAGGAGG ATGTGTTTTG GAGATGCAGA 
CAGAGTATCT TTGATGAAAT GAAGAAGAAA TTTGCACAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTTT TATGTATTAT ACAAGATACT ACTAATTCAA AGACAGTCAA TGAACGAATC 
ACTTTAAATT TACCAGCTTC TACTCCACTG AGGAAACTAT TTGAAGATGT TGCCAACAAA 
GTGGGCTATG TAAATGGAAC CTTTGATTTG GTATGGGGAA ATGGAATCAA TGCTACTGAC 
ATGCCTCCTT TGGATCACAC CAGTGAAAAA TCTCTTCTTG ACACTAACTT TGAACCTGGG 
AAGAAAAACT TTCTGCATTT GACAGATAAA GATGGTGAAC AGCCTCAGAT AGCAGAGGAT 
ACCATTGCAG CAGATGACAG TGTGCAAGAT AGGTTTATAG GCCCTCTTCC AAGAGAAGGT 
TCTGTAGGCT CTACCAATGA TTATGTCGGT CAAAGCTATT CATATTCTTC AATCTTGAAT 
AAATCAGAAA CAGGATATGT GGGATTAGTA AATCAAGCAA TGACTTGCTA TTTGAACAGC 
CTTTTGCAAA CACTTTTCAT GACTCCTGAA TTCAGGAATG CACTGTATAA GTGGGAATTT 
GAGGAATCTG AAGAAGATCC TGTGACAAGC ATTCCATACC AGCTTCAGAG ACTCTTTGTT 
TTATTACAAA CCAGCAAAAA GAGAGCAATT GAAACGACTG ATGTTACAAG AAGCTTTGGA 
TGGGATAGTA GTGAGGCTTG GCAACAACAT GATGTACAAG AACTTTGTAG AGTCATGTTT 
GATGCATTGG AACAGAAATG GAAGCAAACA GAGCAGGCCG ATCTTATAAA TGAACTGTAT 
CAAGGAAAGC TGAAGGACTA CGTGAGATGT CTGGAATGTG GTTATGAGGG CTGGAGAATC 
GACACATATC TTGACATTCC ATTGGTCATC CGACCTTATG GTGCCAGCCA GGCATTTGCT 
AGTGTGGAAG AAGCATTACA AGCATTTATA CAGCCAGAAA TCCTGGATGG CCCCAACCAG 
TACTTTTGTG AGCAGTGTAA AAAGAAGTGT GATGCACGGA AGGGCCTTCG TTTTTTGCAT 
TTCCCATATC TGCTGACTCT CCAGTTGAAA AGGTTTGACT TTGATTACAC CACCATGCAC 
AGGATTAAAT TAAATGACCG GATGACTTTC CCAGAAGAGC TAGATATGAG TACATTTATT 
GATGTTGAAG ATGAGAAGTC TCCTCAGACT GAGAGTTGTA CCGACAGTGG AGCTGAAAAT 
GAAGGCAGTT GTCACAGTGA TCAGATGAGC AACGATTTCT CAAATGATGA TGGGGTCGAT 
GAAGGAATCT GCCTTGAAAG TAATAGTGGA ACTGAAAAGA TCACAAAACC TGGTCTTGAA 
AAGAATTCCT TGCTATACGA ACTCTTTTCT GTCATGGTTC ATTCAGGAAG TGCAGCTGGT 
GGTCATTATT ATGCGTGCAT AAAATCATTC AGTGATGAGC AGTGGTACAG CTTCAATGAT 
CAGCATGTTA GTCGGATAAC CCAAGAGGAC ATTAAGAAGA CTCATGGCGG ATCTTCAGGA 
AGTCGAGGAT ATTATTCTAG TGCCTTTGCC AGCTCCACAA ATGCATATAT GCTTATATAT 
AGATTGAAGG ATCCTTCAAG AAATGCAAAA TTTTTAGAAG TGAAAGATTA CCCAGAGCAC 
ATTAAGAATT TGGTGCGAAA AGAGAAAGAA CTGGAAGAGC AAGAAAAAAA GCAACGTGAA 
ATTGAGCGAA ATACCTGCAA GATAAAATTG TTCTGCATGC ATCCTGTGAA ACAAGTGATG 
ATGGAAAATA AATTGGAGGT TCATAAAGAT AAGACCTTAA AAGAAGCAGT AGAAATAGCT 
TATAAGGTGA TGGATTTAGA AGAAGCCATT CCCCTGGACT GCTGTCGTCT TGTTAAGTAT 
GATGAGTTTC ATGATTATCT AGAGAGGTCA TATGAAGGAG AAGAGGATAC TCCAATGGGA 
CTTCTACTTG GTGGAGTCAA ATCAACATAC ATGTTTGATC TGCTCTTGGA AATCAAAAAA 
CCTGATCAGG TTTTTCAGTC TTATAAACCT GGTGAGGTGA TGGTGAAGGT CCATGTGGTT 
GATATAAAGG CAGAGTCTGT TGCTCCTCCA TTTAGTGTAC GAGCTTATTT AAATCAAACA 
GTCGCCGAGT TTAAGCAACT TATTTCAAAG ACTATACAGC TGCCTGCTGA AACGATGAGA 
ATAGTTCTAG AACGCTGCTA CAATGATTTG CGTCTTCTCA ATGTGCCCAG TAAAACACTG 
AAGGCTGAAG GCTTCTTTAG AAGTAACAAG GTATTTGTCG AAAGTTCTGA GTCTTTGGAT 
CACCAGATGG CCTTTACAGA TTCTCATTTG TGGAAATTGC TGGATCGACA TGCAAATACA 
ATCAGACTGT TTGTTTTATT ACCTGAACAA TCTCCAGGAT CTCATTCTAA AAGGACAGTG 
TACCAGAAAG CTGGTGGGGA TTCTGGCAAC ATGGATGATG CTTGTGAACG AGTTAAAGGA 
CCTCTAGGTA GCCTAAAATC TGTAGAGGCT ATCTTAGAAG AAAGTACTGA AAAGCTTAAA 
AGCTTGTCTC TTCGACAGCA ACAGGATGGA GATAATGGAG ACAGTAGCAA GAGTACAGAG 
ACAAGTGATT TTGAAAACAT TGAATCACCT CTAAATGAGA GAGACTCTTC AGGATCAGTA 
GATACCAGAG AACTTGAACA GCAGATTCCC ACTTCTGATC CAGAAAATTT CCAGTCTGAA 
GAGCGATCCG ACTCTGATGT GAACAATGAC AGGAGTACAA GCTCAGTGGA CAGTGACATT 
CTTAGTTCCA GTCACAGTAG TGACACTTTG TGCAATGCAG ACAGTGCCCC GATCCCTTTG 
GCTAATGGAC TTGACTCTCA TAGTATCACA AGTAGTAGAA GATCTAAGGC AAATGAAGGA 
AAAAAGGAGA CCTGGGATAC AGCTGAAGAA GACTCTGGAA CTGACAGTGA ATATGATGAA 
AGTGGCAAGA GTAGGGGAGA GACACAGTAC ATATATTTCA AAGCAGAATC CTATCCTGCA 
GATGAAGGTT CTGGGGAAGG ACATAAATGG TTGATGGTGC ATGTTGATAA AAGAATTACA 
CTGGCTACCT TCAAACAACA GTTGGAACCC TTTGTTGGAG CTCTGTCTTC TCACTTCAAA 
GTTTTTCGAG TATATGCCAG CAATCAAGAG TTTGAAAGCA TCCGACTGAA TGAAACACTT 
TCATCATTTT CAGATGACAA CAAGATTACA ATTAGATTGG GGAGAGCACT TAAGAAGGGA 
GAGTACAGAG TCAAAGTGTA CCAGCTTTTA GTAAATGAGC AAGAGCCCTG TAGGTTTCTG 
CTTGATGCGG TTTTTGCTAA AGGCATGACT GTTCGACAGT CTAAAGAAGA GCTCATCCCC 
CAGCTCAGGG AGCAGTGTGG GCTAGAAGTC AGCATTGACA GGTTTCGTCT AAGGAAAAAA 
ACATGGAAGA ATCCTGGCAC AGTCTTTTTG GATTATCATG TCTATGAAGA GGATATTAAT 
ATTTCCAGCA ACTGGGAGGT TTTCCTTGAA GTTCTTGATG AAGTGGAGAG GATGAAATCC 
ATGTCACAAT TGGCTGTACT GGCCAGAAGA TGGAGACCAA CAGAGCTGAT ACTGGACCCC 
TTCCAAGAGA TAGTGTTGGA AAGCAGTAGT GTTGATGAAC TGAGAGAGAA GCTCTGCGAA 
ATCAGTGGGA TACCTTTGGA AGATGTGGAA TTTGCAAAGG GAAGAGGAAC ATTTCCCTGT 
GACATGTCTG TCCTAGAAAT TCACCAGGAT TTGGATTGGA ATCCCAAAGT TTCTACATTG 
AATGTGTGGC CCCTTTATAT CTGTGATGAT GGTGCGGTGA TATTTTATAG GGATAAAACT 
GAAGAATTAT TGGAATTAAC AGATGAGCAG AGAAATGAAC TAATGAAAAA GGAAAGCAGC 
CGGCTCCAGA AAACTGGACA TCGTGTAACC TACTCTCCTC GTAAAGAAAA GGCACTAAAA 
ATTTATCTGG ACGGATCATC AAATAAAGAT TTGACTCAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP47 ( XM_031944166.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031800026.1 Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X3, mRNA.

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CloneID	OSa240696
Clone ID Related Accession (Same CDS sequence)	XM_031944166.1
Accession Version	XM_031944166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045431.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCGG GCGAAGAGAA CCAGCTAGTC CCGAAGGAGG ATGTGTTTTG GAGATGCAGA 
CAGAGTATCT TTGATGAAAT GAAGAAGAAA TTTGCACAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTTT TATGTATTAT ACAAGATACT ACTAATTCAA AGACAGTCAA TGAACGAATC 
ACTTTAAATT TACCAGCTTC TACTCCACTG AGGAAACTAT TTGAAGATGT TGCCAACAAA 
GTGGGCTATG TAAATGGAAC CTTTGATTTG GTATGGGGAA ATGGAATCAA TGCTACTGAC 
ATGCCTCCTT TGGATCACAC CAGTGAAAAA TCTCTTCTTG ACACTAACTT TGAACCTGGG 
AAGAAAAACT TTCTGCATTT GACAGATAAA GATGGTGAAC AGCCTCAGAT AGCAGAGGAT 
ACCATTGCAG CAGATGACAG TGTGCAAGAT AGGTTTATAG GCCCTCTTCC AAGAGAAGGT 
TCTGTAGGCT CTACCAATGA TTATGTCGGT CAAAGCTATT CATATTCTTC AATCTTGAAT 
AAATCAGAAA CAGGATATGT GGGATTAGTA AATCAAGCAA TGACTTGCTA TTTGAACAGC 
CTTTTGCAAA CACTTTTCAT GACTCCTGAA TTCAGGAATG CACTGTATAA GTGGGAATTT 
GAGGAATCTG AAGAAGATCC TGTGACAAGC ATTCCATACC AGCTTCAGAG ACTCTTTGTT 
TTATTACAAA CCAGCAAAAA GAGAGCAATT GAAACGACTG ATGTTACAAG AAGCTTTGGA 
TGGGATAGTA GTGAGGCTTG GCAACAACAT GATGTACAAG AACTTTGTAG AGTCATGTTT 
GATGCATTGG AACAGAAATG GAAGCAAACA GAGCAGGCCG ATCTTATAAA TGAACTGTAT 
CAAGGAAAGC TGAAGGACTA CGTGAGATGT CTGGAATGTG GTTATGAGGG CTGGAGAATC 
GACACATATC TTGACATTCC ATTGGTCATC CGACCTTATG GTGCCAGCCA GGCATTTGCT 
AGTGTGGAAG AAGCATTACA AGCATTTATA CAGCCAGAAA TCCTGGATGG CCCCAACCAG 
TACTTTTGTG AGCAGTGTAA AAAGAAGTGT GATGCACGGA AGGGCCTTCG TTTTTTGCAT 
TTCCCATATC TGCTGACTCT CCAGTTGAAA AGGTTTGACT TTGATTACAC CACCATGCAC 
AGGATTAAAT TAAATGACCG GATGACTTTC CCAGAAGAGC TAGATATGAG TACATTTATT 
GATGTTGAAG ATGAGAATTC CTTGCTATAC GAACTCTTTT CTGTCATGGT TCATTCAGGA 
AGTGCAGCTG GTGGTCATTA TTATGCGTGC ATAAAATCAT TCAGTGATGA GCAGTGGTAC 
AGCTTCAATG ATCAGCATGT TAGTCGGATA ACCCAAGAGG ACATTAAGAA GACTCATGGC 
GGATCTTCAG GAAGTCGAGG ATATTATTCT AGTGCCTTTG CCAGCTCCAC AAATGCATAT 
ATGCTTATAT ATAGATTGAA GGATCCTTCA AGAAATGCAA AATTTTTAGA AGTGAAAGAT 
TACCCAGAGC ACATTAAGAA TTTGGTGCGA AAAGAGAAAG AACTGGAAGA GCAAGAAAAA 
AAGCAACGTG AAATTGAGCG AAATACCTGC AAGATAAAAT TGTTCTGCAT GCATCCTGTG 
AAACAAGTGA TGATGGAAAA TAAATTGGAG GTTCATAAAG ATAAGACCTT AAAAGAAGCA 
GTAGAAATAG CTTATAAGGT GATGGATTTA GAAGAAGCCA TTCCCCTGGA CTGCTGTCGT 
CTTGTTAAGT ATGATGAGTT TCATGATTAT CTAGAGAGGT CATATGAAGG AGAAGAGGAT 
ACTCCAATGG GACTTCTACT TGGTGGAGTC AAATCAACAT ACATGTTTGA TCTGCTCTTG 
GAAATCAAAA AACCTGATCA GGTTTTTCAG TCTTATAAAC CTGGTGAGGT GATGGTGAAG 
GTCCATGTGG TTGATATAAA GGCAGAGTCT GTTGCTCCTC CATTTAGTGT ACGAGCTTAT 
TTAAATCAAA CAGTCGCCGA GTTTAAGCAA CTTATTTCAA AGACTATACA GCTGCCTGCT 
GAAACGATGA GAATAGTTCT AGAACGCTGC TACAATGATT TGCGTCTTCT CAATGTGCCC 
AGTAAAACAC TGAAGGCTGA AGGCTTCTTT AGAAGTAACA AGGTATTTGT CGAAAGTTCT 
GAGTCTTTGG ATCACCAGAT GGCCTTTACA GATTCTCATT TGTGGAAATT GCTGGATCGA 
CATGCAAATA CAATCAGACT GTTTGTTTTA TTACCTGAAC AATCTCCAGG ATCTCATTCT 
AAAAGGACAG TGTACCAGAA AGCTGGTGGG GATTCTGGCA ACATGGATGA TGCTTGTGAA 
CGAGTTAAAG GACCTCTAGG TAGCCTAAAA TCTGTAGAGG CTATCTTAGA AGAAAGTACT 
GAAAAGCTTA AAAGCTTGTC TCTTCGACAG CAACAGGATG GAGATAATGG AGACAGTAGC 
AAGAGTACAG AGACAAGTGA TTTTGAAAAC ATTGAATCAC CTCTAAATGA GAGAGACTCT 
TCAGGATCAG TAGATACCAG AGAACTTGAA CAGCAGATTC CCACTTCTGA TCCAGAAAAT 
TTCCAGTCTG AAGAGCGATC CGACTCTGAT GTGAACAATG ACAGGAGTAC AAGCTCAGTG 
GACAGTGACA TTCTTAGTTC CAGTCACAGT AGTGACACTT TGTGCAATGC AGACAGTGCC 
CCGATCCCTT TGGCTAATGG ACTTGACTCT CATAGTATCA CAAGTAGTAG AAGATCTAAG 
GCAAATGAAG GAAAAAAGGA GACCTGGGAT ACAGCTGAAG AAGACTCTGG AACTGACAGT 
GAATATGATG AAAGTGGCAA GAGTAGGGGA GAGACACAGT ACATATATTT CAAAGCAGAA 
TCCTATCCTG CAGATGAAGG TTCTGGGGAA GGACATAAAT GGTTGATGGT GCATGTTGAT 
AAAAGAATTA CACTGGCTAC CTTCAAACAA CAGTTGGAAC CCTTTGTTGG AGCTCTGTCT 
TCTCACTTCA AAGTTTTTCG AGTATATGCC AGCAATCAAG AGTTTGAAAG CATCCGACTG 
AATGAAACAC TTTCATCATT TTCAGATGAC AACAAGATTA CAATTAGATT GGGGAGAGCA 
CTTAAGAAGG GAGAGTACAG AGTCAAAGTG TACCAGCTTT TAGTAAATGA GCAAGAGCCC 
TGTAGGTTTC TGCTTGATGC GGTTTTTGCT AAAGGCATGA CTGTTCGACA GTCTAAAGAA 
GAGCTCATCC CCCAGCTCAG GGAGCAGTGT GGGCTAGAAG TCAGCATTGA CAGGTTTCGT 
CTAAGGAAAA AAACATGGAA GAATCCTGGC ACAGTCTTTT TGGATTATCA TGTCTATGAA 
GAGGATATTA ATATTTCCAG CAACTGGGAG GTTTTCCTTG AAGTTCTTGA TGAAGTGGAG 
AGGATGAAAT CCATGTCACA ATTGGCTGTA CTGGCCAGAA GATGGAGACC AACAGAGCTG 
ATACTGGACC CCTTCCAAGA GATAGTGTTG GAAAGCAGTA GTGTTGATGA ACTGAGAGAG 
AAGCTCTGCG AAATCAGTGG GATACCTTTG GAAGATGTGG AATTTGCAAA GGGAAGAGGA 
ACATTTCCCT GTGACATGTC TGTCCTAGAA ATTCACCAGG ATTTGGATTG GAATCCCAAA 
GTTTCTACAT TGAATGTGTG GCCCCTTTAT ATCTGTGATG ATGGTGCGGT GATATTTTAT 
AGGGATAAAA CTGAAGAATT ATTGGAATTA ACAGATGAGC AGAGAAATGA ACTAATGAAA 
AAGGAAAGCA GCCGGCTCCA GAAAACTGGA CATCGTGTAA CCTACTCTCC TCGTAAAGAA 
AAGGCACTAA AAATTTATCT GGACGGATCA TCAAATAAAG ATTTGACTCA AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031800026.1
CDS	200..4156
Translation	MVPGEENQLVPKEDVFWRCRQSIFDEMKKKFAQIENAAEEPRVLCIIQDTTNSKTVNERITLNLPASTPLRKLFEDVANKVGYVNGTFDLVWGNGINATDMPPLDHTSEKSLLDTNFEPGKKNFLHLTDKDGEQPQIAEDTIAADDSVQDRFIGPLPREGSVGSTNDYVGQSYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEESEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGASQAFASVEEALQAFIQPEILDGPNQYFCEQCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMSTFIDVEDENSLLYELFSVMVHSGSAAGGHYYACIKSFSDEQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPSRNAKFLEVKDYPEHIKNLVRKEKELEEQEKKQREIERNTCKIKLFCMHPVKQVMMENKLEVHKDKTLKEAVEIAYKVMDLEEAIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLEIKKPDQVFQSYKPGEVMVKVHVVDIKAESVAPPFSVRAYLNQTVAEFKQLISKTIQLPAETMRIVLERCYNDLRLLNVPSKTLKAEGFFRSNKVFVESSESLDHQMAFTDSHLWKLLDRHANTIRLFVLLPEQSPGSHSKRTVYQKAGGDSGNMDDACERVKGPLGSLKSVEAILEESTEKLKSLSLRQQQDGDNGDSSKSTETSDFENIESPLNERDSSGSVDTRELEQQIPTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSAPIPLANGLDSHSITSSRRSKANEGKKETWDTAEEDSGTDSEYDESGKSRGETQYIYFKAESYPADEGSGEGHKWLMVHVDKRITLATFKQQLEPFVGALSSHFKVFRVYASNQEFESIRLNETLSSFSDDNKITIRLGRALKKGEYRVKVYQLLVNEQEPCRFLLDAVFAKGMTVRQSKEELIPQLREQCGLEVSIDRFRLRKKTWKNPGTVFLDYHVYEEDINISSNWEVFLEVLDEVERMKSMSQLAVLARRWRPTELILDPFQEIVLESSSVDELREKLCEISGIPLEDVEFAKGRGTFPCDMSVLEIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDKTEELLELTDEQRNELMKKESSRLQKTGHRVTYSPRKEKALKIYLDGSSNKDLTQD

Target ORF information:

RefSeq Version	XM_031944166.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ubiquitin specific peptidase 47 (USP47), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031944166.1

ORF Insert Sequence:

ATGGTCCCGG GCGAAGAGAA CCAGCTAGTC CCGAAGGAGG ATGTGTTTTG GAGATGCAGA 
CAGAGTATCT TTGATGAAAT GAAGAAGAAA TTTGCACAGA TAGAAAATGC TGCTGAAGAA 
CCTAGAGTTT TATGTATTAT ACAAGATACT ACTAATTCAA AGACAGTCAA TGAACGAATC 
ACTTTAAATT TACCAGCTTC TACTCCACTG AGGAAACTAT TTGAAGATGT TGCCAACAAA 
GTGGGCTATG TAAATGGAAC CTTTGATTTG GTATGGGGAA ATGGAATCAA TGCTACTGAC 
ATGCCTCCTT TGGATCACAC CAGTGAAAAA TCTCTTCTTG ACACTAACTT TGAACCTGGG 
AAGAAAAACT TTCTGCATTT GACAGATAAA GATGGTGAAC AGCCTCAGAT AGCAGAGGAT 
ACCATTGCAG CAGATGACAG TGTGCAAGAT AGGTTTATAG GCCCTCTTCC AAGAGAAGGT 
TCTGTAGGCT CTACCAATGA TTATGTCGGT CAAAGCTATT CATATTCTTC AATCTTGAAT 
AAATCAGAAA CAGGATATGT GGGATTAGTA AATCAAGCAA TGACTTGCTA TTTGAACAGC 
CTTTTGCAAA CACTTTTCAT GACTCCTGAA TTCAGGAATG CACTGTATAA GTGGGAATTT 
GAGGAATCTG AAGAAGATCC TGTGACAAGC ATTCCATACC AGCTTCAGAG ACTCTTTGTT 
TTATTACAAA CCAGCAAAAA GAGAGCAATT GAAACGACTG ATGTTACAAG AAGCTTTGGA 
TGGGATAGTA GTGAGGCTTG GCAACAACAT GATGTACAAG AACTTTGTAG AGTCATGTTT 
GATGCATTGG AACAGAAATG GAAGCAAACA GAGCAGGCCG ATCTTATAAA TGAACTGTAT 
CAAGGAAAGC TGAAGGACTA CGTGAGATGT CTGGAATGTG GTTATGAGGG CTGGAGAATC 
GACACATATC TTGACATTCC ATTGGTCATC CGACCTTATG GTGCCAGCCA GGCATTTGCT 
AGTGTGGAAG AAGCATTACA AGCATTTATA CAGCCAGAAA TCCTGGATGG CCCCAACCAG 
TACTTTTGTG AGCAGTGTAA AAAGAAGTGT GATGCACGGA AGGGCCTTCG TTTTTTGCAT 
TTCCCATATC TGCTGACTCT CCAGTTGAAA AGGTTTGACT TTGATTACAC CACCATGCAC 
AGGATTAAAT TAAATGACCG GATGACTTTC CCAGAAGAGC TAGATATGAG TACATTTATT 
GATGTTGAAG ATGAGAATTC CTTGCTATAC GAACTCTTTT CTGTCATGGT TCATTCAGGA 
AGTGCAGCTG GTGGTCATTA TTATGCGTGC ATAAAATCAT TCAGTGATGA GCAGTGGTAC 
AGCTTCAATG ATCAGCATGT TAGTCGGATA ACCCAAGAGG ACATTAAGAA GACTCATGGC 
GGATCTTCAG GAAGTCGAGG ATATTATTCT AGTGCCTTTG CCAGCTCCAC AAATGCATAT 
ATGCTTATAT ATAGATTGAA GGATCCTTCA AGAAATGCAA AATTTTTAGA AGTGAAAGAT 
TACCCAGAGC ACATTAAGAA TTTGGTGCGA AAAGAGAAAG AACTGGAAGA GCAAGAAAAA 
AAGCAACGTG AAATTGAGCG AAATACCTGC AAGATAAAAT TGTTCTGCAT GCATCCTGTG 
AAACAAGTGA TGATGGAAAA TAAATTGGAG GTTCATAAAG ATAAGACCTT AAAAGAAGCA 
GTAGAAATAG CTTATAAGGT GATGGATTTA GAAGAAGCCA TTCCCCTGGA CTGCTGTCGT 
CTTGTTAAGT ATGATGAGTT TCATGATTAT CTAGAGAGGT CATATGAAGG AGAAGAGGAT 
ACTCCAATGG GACTTCTACT TGGTGGAGTC AAATCAACAT ACATGTTTGA TCTGCTCTTG 
GAAATCAAAA AACCTGATCA GGTTTTTCAG TCTTATAAAC CTGGTGAGGT GATGGTGAAG 
GTCCATGTGG TTGATATAAA GGCAGAGTCT GTTGCTCCTC CATTTAGTGT ACGAGCTTAT 
TTAAATCAAA CAGTCGCCGA GTTTAAGCAA CTTATTTCAA AGACTATACA GCTGCCTGCT 
GAAACGATGA GAATAGTTCT AGAACGCTGC TACAATGATT TGCGTCTTCT CAATGTGCCC 
AGTAAAACAC TGAAGGCTGA AGGCTTCTTT AGAAGTAACA AGGTATTTGT CGAAAGTTCT 
GAGTCTTTGG ATCACCAGAT GGCCTTTACA GATTCTCATT TGTGGAAATT GCTGGATCGA 
CATGCAAATA CAATCAGACT GTTTGTTTTA TTACCTGAAC AATCTCCAGG ATCTCATTCT 
AAAAGGACAG TGTACCAGAA AGCTGGTGGG GATTCTGGCA ACATGGATGA TGCTTGTGAA 
CGAGTTAAAG GACCTCTAGG TAGCCTAAAA TCTGTAGAGG CTATCTTAGA AGAAAGTACT 
GAAAAGCTTA AAAGCTTGTC TCTTCGACAG CAACAGGATG GAGATAATGG AGACAGTAGC 
AAGAGTACAG AGACAAGTGA TTTTGAAAAC ATTGAATCAC CTCTAAATGA GAGAGACTCT 
TCAGGATCAG TAGATACCAG AGAACTTGAA CAGCAGATTC CCACTTCTGA TCCAGAAAAT 
TTCCAGTCTG AAGAGCGATC CGACTCTGAT GTGAACAATG ACAGGAGTAC AAGCTCAGTG 
GACAGTGACA TTCTTAGTTC CAGTCACAGT AGTGACACTT TGTGCAATGC AGACAGTGCC 
CCGATCCCTT TGGCTAATGG ACTTGACTCT CATAGTATCA CAAGTAGTAG AAGATCTAAG 
GCAAATGAAG GAAAAAAGGA GACCTGGGAT ACAGCTGAAG AAGACTCTGG AACTGACAGT 
GAATATGATG AAAGTGGCAA GAGTAGGGGA GAGACACAGT ACATATATTT CAAAGCAGAA 
TCCTATCCTG CAGATGAAGG TTCTGGGGAA GGACATAAAT GGTTGATGGT GCATGTTGAT 
AAAAGAATTA CACTGGCTAC CTTCAAACAA CAGTTGGAAC CCTTTGTTGG AGCTCTGTCT 
TCTCACTTCA AAGTTTTTCG AGTATATGCC AGCAATCAAG AGTTTGAAAG CATCCGACTG 
AATGAAACAC TTTCATCATT TTCAGATGAC AACAAGATTA CAATTAGATT GGGGAGAGCA 
CTTAAGAAGG GAGAGTACAG AGTCAAAGTG TACCAGCTTT TAGTAAATGA GCAAGAGCCC 
TGTAGGTTTC TGCTTGATGC GGTTTTTGCT AAAGGCATGA CTGTTCGACA GTCTAAAGAA 
GAGCTCATCC CCCAGCTCAG GGAGCAGTGT GGGCTAGAAG TCAGCATTGA CAGGTTTCGT 
CTAAGGAAAA AAACATGGAA GAATCCTGGC ACAGTCTTTT TGGATTATCA TGTCTATGAA 
GAGGATATTA ATATTTCCAG CAACTGGGAG GTTTTCCTTG AAGTTCTTGA TGAAGTGGAG 
AGGATGAAAT CCATGTCACA ATTGGCTGTA CTGGCCAGAA GATGGAGACC AACAGAGCTG 
ATACTGGACC CCTTCCAAGA GATAGTGTTG GAAAGCAGTA GTGTTGATGA ACTGAGAGAG 
AAGCTCTGCG AAATCAGTGG GATACCTTTG GAAGATGTGG AATTTGCAAA GGGAAGAGGA 
ACATTTCCCT GTGACATGTC TGTCCTAGAA ATTCACCAGG ATTTGGATTG GAATCCCAAA 
GTTTCTACAT TGAATGTGTG GCCCCTTTAT ATCTGTGATG ATGGTGCGGT GATATTTTAT 
AGGGATAAAA CTGAAGAATT ATTGGAATTA ACAGATGAGC AGAGAAATGA ACTAATGAAA 
AAGGAAAGCA GCCGGCTCCA GAAAACTGGA CATCGTGTAA CCTACTCTCC TCGTAAAGAA 
AAGGCACTAA AAATTTATCT GGACGGATCA TCAAATAAAG ATTTGACTCA AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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USP47 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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