HEATR5A cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	HEATR5A
Entrez Gene ID	100917290
Full Name	HEAT repeat containing 5A
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031952257.1	XP_031808117.1	HEAT repeat-containing protein 5A isoform X1
XM_023501135.1	XP_023356903.1	HEAT repeat-containing protein 5A
XM_031952256.1	XP_031808116.1	HEAT repeat-containing protein 5A isoform X1
XM_031952259.1	XP_031808119.1	HEAT repeat-containing protein 5A isoform X1
XM_023501135.2	XP_023356903.1	HEAT repeat-containing protein 5A isoform X2
XM_012546981.2	XP_012402435.1	HEAT repeat-containing protein 5A
XM_012546981.1	XP_012402435.1	HEAT repeat-containing protein 5A
XM_031952260.1	XP_031808120.1	HEAT repeat-containing protein 5A isoform X1

Pathways from BioSystems
Gene Ontology

The following HEATR5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEATR5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa117582	XM_023501135.2 Latest version!	Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa117582	XM_012546981.2 Latest version!	Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa225025	XM_031952260.1 Latest version!	Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa225025	XM_031952259.1 Latest version!	Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa225025	XM_031952257.1 Latest version!	Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa225025	XM_031952256.1 Latest version!	Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OSa117582	XM_023501135.1	Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa117582	XM_012546981.1	Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HEATR5A ( XM_023501135.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023356903.1 Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa117582
Clone ID Related Accession (Same CDS sequence)	XM_023501135.1 , XM_023501135.2 , XM_012546981.2 , XM_012546981.1
Accession Version	XM_023501135.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 5A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816524.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG CTCACAGCTT ATTGCTGAAT GAAGAAGCAT ATAATCAACT TGGAGAAGTT 
CAGAAGGCAG AATTTGTTTT TGAATGGTTG AAATTTTTAG AGAAACTTTT GCTAGCAACC 
AACAAGGCTG ACATAAAGGA GAAACAGAAG AGTATTATTG AGCAGCTCAT ATCTCTATTA 
AATAGCTCTC CAGGGCCACC TACCCGAAAG CTTCTTGCTG AGAACCTAGC TGTTCTTTAT 
AGTATTGGAG ATACATTTTC TGTTTATCAA ACAATTGACA AATGTAATGA ACTCATTCGT 
AGCAAAGATG ACTCTCCAAG TTATCTTCCC ACAAAACTTG CTGCTGTGGT ATGTCTGGGT 
TCTTTGTATA AGAAGTTGGG GAGACTATTG GGTAACACCT TTATAGATAC AGTGGGGAAT 
ATACTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGTCGAT ATGAGATTAT GCTGAGTTTG 
CAAAATATAC TGAGTGGACT TGGAGCTGCT GCTGCCCCTT GTCACAGAGA CATTTATAAA 
GCTGCAAGGT CATGTTTGAC AGACAGGTCC ATGGCAGTTC GCTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAGAATGA AGCTAGCTTT ATGTGGAGTA CAGACATAGA CAGTGTTGCA 
ACTTTGTGTT TTAAGTCATT TGAAGGTTCC AACTATGATG TGCGAATAGC AGTATCAAAG 
CTCTTAGGTA CAGTGTTGGC TAGAGCTGTA ATGTCCAAAC CTGGAACAGC AACTTCCCGG 
CAAAATGTTC GCAAAGTTTC TCTGGAGGAA GCCATGGAGT TACTAGGAAC AGGGTTTCTA 
CGTGGGAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGT TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG TGTTACTCAG GCATATGTGG TGTTTATTTC AACACTAGGA 
GGACGTTGGT TAGAAAGAAA TTTTTCCATC TTACTCTCTC ATATCCTTAG CCTAGTATCA 
CAGTTTCACC CTAAAGCAAT CCAAACTCAG ATGGATGCTA TCTGTTGTCG TCGCTGTGTT 
TCATTTATCC TTCGAACTAC TATAGGAAGC CTTCTCGGGG AAAAAGCTCA AATTGCTGCT 
GCCAGGGATA TTTGTCAGGT CATCTGGAAA CTAAAGAAAA CTGTTGATGC TGTAATGAGT 
GAAGTTAATT TGGAAACTCG ACCTGGCTCC ACAGATGTTG CTGCCAGCCA GCATGTGCTA 
GTATGTGCTT TACAAGAACT TGGAAATCTA ATATATGGTC TTGGCACAAC AGCTGCACCT 
TTATTACAGG ATTCAAGTGC AGGGATTCTT GACAATGTCA TTTCAGTTAT TGTTCATCCC 
AGCATTTCAG TTCGATTAGC AGCAGCTTGG TGTTTACACT GCATTGCTGT AGCATTGCCT 
TCCTACCTAA CTCCACTTTT GGACAGTTGC CTTGAACGAC TTACTGTGCT CAAATCCTCA 
CCTGAAGCTG TGACTGGCTT TAGTTTTGCT GTGGCAGCTC TACTAGGAGC AGTGAAATAT 
TGCCCTTTAG GAATTCCACA TGGAAAAGGA AAGATAATTG TGATGTTAGC AGAGGATTTG 
TTATGTTCTG CTGCTCAGAA TAGTCGCCTT TCAGTTCAAC GAACACAAGC TGGATGGTTG 
CTGATAGCTG CCTTAATGAC ATTAGGCTCT GCATTTATTC ATCACCACCT TCCCCGGGTG 
CTAAGATTAT GGAAATGTGT TTTTCCACTA TCTCCTAAGG ATCTAGAAAC AGAAAGAAGT 
CGAGGAGACT CATTTACATG GCAGGTGACC TTGGAAGGTC GTGCAGGGGC CTTCTGTGCT 
ATCAAGAGCT TTGTTTCCCA CTGTAATGAT CTTCTTACTG ATGAAGTCAT TCAGAGACTT 
CTTCCTCCCC TTCCGTGTGC TGTTAATTTA TTAACTCAAC TTTCTTCAAT ACAGAAAACA 
TATGGAAGTC CTTTAAAAAC TCCTTCAGTG GTTTACAAAC AAAGACTTTA TGAACTATTG 
GCTTTATTAC CTCCTGAGAT CTATGCAGGA AGCCTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACCTGA CTTCTTCTGA TACCCAGATG GCAGCATCCA CTTTCTTACT TCCACCCCTC 
TGTCACCAAG ATGATCTTTT ATTACTAAGT CCTTTGCTAC AAGACACTGA CCACAGATTT 
ATTGAAGAAC AGCTCCTCCT TGGTAATGGT GTTGCTTGTG GAAGTCTAGA GTATGACCCA 
TTTTCAGTTT ATGAACATGG CATAGAAGGG GATTCAGTAC CTAAGCCCCT ACCTCCAATG 
TTGTCACTTA TCAGCTCTGC AGCCAGGCTT TTTGGAATTG TATGTGCCCA TGTAGCAGAA 
TCTCAAAGGC TCTTGGTATT GGAACAACTT TTGGACAAAA TAAAACACAC AAAAGCATCT 
CGTCAACAAG TAGTACAATT ACATGTTATG GCAGCATTTT CTAATTTTTT GAAGTATGTG 
GCCAACTCCA AAGGAAATTT AGGCTCAGAA GAAATTAGAA GACCTGCACT AACATTATTG 
ATGGGAGCCT TGGAAAGTAC CAACCCCTTA ATGCGTTGTA CTGCTGCAGA AGGTTTGGCT 
AGATTAGCCC AAGTGATTGG TGATGGCTCC TTCACTGCTG GATTAGCACA GGTTAGCTTT 
GAAAAACTGA AATCAGCTAG GGATGTGGTA ACCAGAACAG GACATTCTTT GGCTCTGGGA 
TCCTTACATA GATATTTAGG AGGAGTGGGC TCTTCTCAGC ACTTAAATGC TTGCATCGGA 
ATACTTTACA CTCTGTCACA GGATGGAACT TCTCCTGATG TACAGACATG GGCCCTACAT 
TCTCTCTCAT TGATAATTGA TTCTGCTGGT CCACTGTACC ATTTACATGT GGAATCTACC 
CTCTCTCTTA TACTAATGTT GTTGTTGACT GTTCCACCTA CTCATGCTGA AGTTCACCAA 
AGTCTTGGAC GATGTTTGAA TGTACTCATC ACCACATTGG GTCCAGAACT ACAAGGTAGC 
AGTACTGCAG TTTCTACTTT ACGGACATCC TGTCTTTTGG GGTGTGCAAT AATGCAGGAT 
AACCCAGATT GCCTTGTTCA AGCTCAAGCC ATCTCTTGTC TTCAACAACT TCATATGTTT 
GCCCCACGCT ATGTCAATTT ATCTAGTCTA GTCAGCTGTC TTTGTGTGAA TCTCTGTAGC 
TCCTACTTGT TTCTGAGAAG AGCAGTACTA GCTTGTTTAC GTCAAATTGT GCAAAGGGAA 
GCAGCTGAGG TTTCAGAACA TGCTGTTGTT CTTGCTAAGG ACAGCAGAGA AGACTTAATT 
CCAGATGCTA ATATCCGAGA AGTGGGTCTT GAGGGGGCAT TGTTGAACTT ACTAGACAAA 
GAATCTGATC AGAGATTGTG CCAGGATATC AAAGAGACCC TGAGTCATAT GATCACATCA 
ATGGCAGTGG GAAAGCTTTC TTTTTGGCTA AAACTTTGTA AAGATGTCCT TGCTTCATCA 
GCTGATTTTA CAACTGTTGC TTCTGTGGAT ACAACTCAGG ATGATGAAAT AGATAAAGAA 
GATGATGCTT CAGTCCTAAC CTACAGAAAT GATGAGAAAT GCCGGCCAAT TACAAATCCA 
CGATGGGCTA CTAGAGTTTT TGCTGCTGAA TGTGTGTGTA AAATAATCAG CCAGTGTGAG 
AATTCAGACA GTGCTCACTT TGACATTGCT TTGGCTCAAG AAATGAAAGA AAGGGATTCA 
CGAAATGATT TTTTGGTATT ACATCTTGCT GACTTGATCC GCATGGCTTT TATGGCTGCA 
ACAGACCACA GTGACCAGCT CCGTCTTTCT GGTCTTCAGA CACTGTTAGT AGTTGTTCGG 
CAATTTGCAA CTATTCCTGA ACCAGAATTC CCAGGTCATG TGATTCTGGA ACAGTATCAA 
GCCAATGTGG GAGCAGCACT TAGACCAGCT TTTACTTCAG ATACACCACC AGATGTAACT 
GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG ATAGCAAGTG GAGTTGTAAG TGATCTTAAT 
GATCTCCGAA GAGTTCATCA ATTGCTTGTA TCATCCTTGA CGAAAATACA GGCTGGAAAA 
GAAGCCTTGA GTCAATTATA TAACGAAAGT GCTTCTACTA TGGAAATCCT AGCTGTTCTC 
AAAGCCTGGG CAGAGGTTTA CATAATTGCT GTAGAAAGAC AGAAAAAACA AACTGAGAAA 
CAGAAGCAGA CTATGAAAAT TGTTGGCTCT ATAGAGGAAA ATGCCAGAGA TGTGTCATCT 
TCCATAGATG GACTTCTTGA CTTAGTCCAA ACAGATCTTG GAACATTAAG CAAACTCTGG 
CTTGCTGCTC TTCAAGATTT TGCTCTTCTA ACTTTGCCTT CAGAATTTGC TTCCCAACTT 
CCTCTTGAAG GTGGAGCTTT CTACACTGCA GAAACTAGTG AAAATGCAAA GCCACACTAT 
CATAATTCGT GGGCTCTTAT TCTTCATGCT ACAGCATTGT GGCTCACTAG CACAGGCTTC 
ATTGTAGCTG ACCCAGATGA AGGAGCAACT AATCTCTCAA GACCTGTAAC ACCGACTACA 
ATGTGTCAGG GTTCTTCTGG GGCATCAGTG AAATCCCCTG AGGATGTTTA TACAGACAGA 
TTTCACCTTA TCTTGGGAAT CAGTGTGGAA TTTCTCTGTT CCCTGCGTTC AGATGCAACC 
ATGGAAAGCA TCACTGCTTG TTTACATGCA CTGCAGGCTT TACTGGATGT TCCTTGGCCC 
AGATCTAAGA TTGGCAGTGA CCAGGACTTG GGAGTTGAGT TGTTGAATGT ACTCCATCGA 
GTAATACTGA CTAGAGAGTC ACCTTCCATT CAGTTAGCTT CACTTGAAGT TGTAAGACAG 
GTTATCTACG CAGCCCAAGA ACATGTAAAG GAAAAGAGGA GAAGTGCAGA AGTTGATGAT 
GGAGCTGCTG AAAAGGAAAC CCTGCCAGAA TTTGGAGAAG GAAAGGATAC GGGAGGACTG 
GTACCTGGAA GATCTCTGGT GTTTGCAACA TTGGAGCTAT GTGTATGCAT TTTGGTCCGG 
CAACTTCCAG AGTTAAATCC TAAACTGACC GGTAGTCCAG GAATAAAAAC TACAAAAGCA 
CAGATTCTTT CAGATGATGG AAATAAGTTG GTTTCAGCAG CATTAACTAT CCTCTCAGAA 
CTTCCTGGAG TATGCTCTCC CGAAGGAAGC ATTTCAGTTC TCCCTACTAT TCTGTACCTT 
ATAATTGGGG TCCTCCGAGA AACTGCTGTG AGGTTACCTG GAGGACAGTT ATCTTATACT 
GTTGGAGCTG CTCTACAAGC CTTGAAAGGA ATATTGGCAT CTCCCTTGGC TCGAGCAGAG 
AAGAGTCACA CTGCTTGGAC TGACCTTCTT CGAAGTGCTT TGACAACAAT CCTAGACTGC 
TGGGATCCAG ATGGAACACA GCAAGAGTTT GATGAAATCA GCCTGCTTAC TGCCATAACA 
GTATTTATCT TGTCTTCAAG TCCAGAAGTG ACCACCATCC AGTGCCTGCA AGAACGTTGT 
ATACAGAAGT TTAAAGCTAC TCTTGAGTTA AAAGACCCAG TAGTACAAAT AAAGTGCTAT 
CAGCTACTGC TTTCCATTTT CCAGTATCCA AACCCAGCAG TATCCTACCC TTATATTCAT 
TCATTAGCAT CATCTATTGT GAGAAAACTG CAAGAGACAG ATAAAGTAAA ACCAGAAGAT 
GCTGCTGAGC TTCAGGTGAT TCAAGAAGGT ATTAAGGTCT TAGAAGCCCT AGTAGGGATT 
GCGGAAGAAC AGCACCGTGC TCACTTGGTG GCCGACATTC TCCCTATTCT TATTTCCTTT 
CTTTTGGATG AAAACACTCT GGGCTCAGCA CCCACCACAA TGAGAAATTT ACATGACTTT 
GCTTTGCAGA ATCTGATGCA GATTGGTCCC CAGTATTCAT CAACTTTTAA AAATATTATG 
GCTTCTTCTC CCACAATGAA AGCCCGTCTT GAGGCAGCTA TAAAAGGCAA TCAGGAAAAT 
ATCAAAATCA AGACAACCCC AAAACATATC ACAAAACCTG GAAAAAATTC AAGTATTCAG 
TTGAAGACCA ATTTTTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023356903.1
CDS	246..6386
Translation	MELAHSLLLNEEAYNQLGEVQKAEFVFEWLKFLEKLLLATNKADIKEKQKSIIEQLISLLNSSPGPPTRKLLAENLAVLYSIGDTFSVYQTIDKCNELIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRLLGNTFIDTVGNILKAMKSAESQGRYEIMLSLQNILSGLGAAAAPCHRDIYKAARSCLTDRSMAVRCAAAKCLLELQNEASFMWSTDIDSVATLCFKSFEGSNYDVRIAVSKLLGTVLARAVMSKPGTATSRQNVRKVSLEEAMELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFISTLGGRWLERNFSILLSHILSLVSQFHPKAIQTQMDAICCRRCVSFILRTTIGSLLGEKAQIAAARDICQVIWKLKKTVDAVMSEVNLETRPGSTDVAASQHVLVCALQELGNLIYGLGTTAAPLLQDSSAGILDNVISVIVHPSISVRLAAAWCLHCIAVALPSYLTPLLDSCLERLTVLKSSPEAVTGFSFAVAALLGAVKYCPLGIPHGKGKIIVMLAEDLLCSAAQNSRLSVQRTQAGWLLIAALMTLGSAFIHHHLPRVLRLWKCVFPLSPKDLETERSRGDSFTWQVTLEGRAGAFCAIKSFVSHCNDLLTDEVIQRLLPPLPCAVNLLTQLSSIQKTYGSPLKTPSVVYKQRLYELLALLPPEIYAGSLCAILKELAADLTSSDTQMAASTFLLPPLCHQDDLLLLSPLLQDTDHRFIEEQLLLGNGVACGSLEYDPFSVYEHGIEGDSVPKPLPPMLSLISSAARLFGIVCAHVAESQRLLVLEQLLDKIKHTKASRQQVVQLHVMAAFSNFLKYVANSKGNLGSEEIRRPALTLLMGALESTNPLMRCTAAEGLARLAQVIGDGSFTAGLAQVSFEKLKSARDVVTRTGHSLALGSLHRYLGGVGSSQHLNACIGILYTLSQDGTSPDVQTWALHSLSLIIDSAGPLYHLHVESTLSLILMLLLTVPPTHAEVHQSLGRCLNVLITTLGPELQGSSTAVSTLRTSCLLGCAIMQDNPDCLVQAQAISCLQQLHMFAPRYVNLSSLVSCLCVNLCSSYLFLRRAVLACLRQIVQREAAEVSEHAVVLAKDSREDLIPDANIREVGLEGALLNLLDKESDQRLCQDIKETLSHMITSMAVGKLSFWLKLCKDVLASSADFTTVASVDTTQDDEIDKEDDASVLTYRNDEKCRPITNPRWATRVFAAECVCKIISQCENSDSAHFDIALAQEMKERDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLQTLLVVVRQFATIPEPEFPGHVILEQYQANVGAALRPAFTSDTPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSQLYNESASTMEILAVLKAWAEVYIIAVERQKKQTEKQKQTMKIVGSIEENARDVSSSIDGLLDLVQTDLGTLSKLWLAALQDFALLTLPSEFASQLPLEGGAFYTAETSENAKPHYHNSWALILHATALWLTSTGFIVADPDEGATNLSRPVTPTTMCQGSSGASVKSPEDVYTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGVELLNVLHRVILTRESPSIQLASLEVVRQVIYAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGRSLVFATLELCVCILVRQLPELNPKLTGSPGIKTTKAQILSDDGNKLVSAALTILSELPGVCSPEGSISVLPTILYLIIGVLRETAVRLPGGQLSYTVGAALQALKGILASPLARAEKSHTAWTDLLRSALTTILDCWDPDGTQQEFDEISLLTAITVFILSSSPEVTTIQCLQERCIQKFKATLELKDPVVQIKCYQLLLSIFQYPNPAVSYPYIHSLASSIVRKLQETDKVKPEDAAELQVIQEGIKVLEALVGIAEEQHRAHLVADILPILISFLLDENTLGSAPTTMRNLHDFALQNLMQIGPQYSSTFKNIMASSPTMKARLEAAIKGNQENIKIKTTPKHITKPGKNSSIQLKTNFF

Target ORF information:

RefSeq Version	XM_023501135.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023501135.1

ORF Insert Sequence:

ATGGAACTGG CTCACAGCTT ATTGCTGAAT GAAGAAGCAT ATAATCAACT TGGAGAAGTT 
CAGAAGGCAG AATTTGTTTT TGAATGGTTG AAATTTTTAG AGAAACTTTT GCTAGCAACC 
AACAAGGCTG ACATAAAGGA GAAACAGAAG AGTATTATTG AGCAGCTCAT ATCTCTATTA 
AATAGCTCTC CAGGGCCACC TACCCGAAAG CTTCTTGCTG AGAACCTAGC TGTTCTTTAT 
AGTATTGGAG ATACATTTTC TGTTTATCAA ACAATTGACA AATGTAATGA ACTCATTCGT 
AGCAAAGATG ACTCTCCAAG TTATCTTCCC ACAAAACTTG CTGCTGTGGT ATGTCTGGGT 
TCTTTGTATA AGAAGTTGGG GAGACTATTG GGTAACACCT TTATAGATAC AGTGGGGAAT 
ATACTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGTCGAT ATGAGATTAT GCTGAGTTTG 
CAAAATATAC TGAGTGGACT TGGAGCTGCT GCTGCCCCTT GTCACAGAGA CATTTATAAA 
GCTGCAAGGT CATGTTTGAC AGACAGGTCC ATGGCAGTTC GCTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAGAATGA AGCTAGCTTT ATGTGGAGTA CAGACATAGA CAGTGTTGCA 
ACTTTGTGTT TTAAGTCATT TGAAGGTTCC AACTATGATG TGCGAATAGC AGTATCAAAG 
CTCTTAGGTA CAGTGTTGGC TAGAGCTGTA ATGTCCAAAC CTGGAACAGC AACTTCCCGG 
CAAAATGTTC GCAAAGTTTC TCTGGAGGAA GCCATGGAGT TACTAGGAAC AGGGTTTCTA 
CGTGGGAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGT TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG TGTTACTCAG GCATATGTGG TGTTTATTTC AACACTAGGA 
GGACGTTGGT TAGAAAGAAA TTTTTCCATC TTACTCTCTC ATATCCTTAG CCTAGTATCA 
CAGTTTCACC CTAAAGCAAT CCAAACTCAG ATGGATGCTA TCTGTTGTCG TCGCTGTGTT 
TCATTTATCC TTCGAACTAC TATAGGAAGC CTTCTCGGGG AAAAAGCTCA AATTGCTGCT 
GCCAGGGATA TTTGTCAGGT CATCTGGAAA CTAAAGAAAA CTGTTGATGC TGTAATGAGT 
GAAGTTAATT TGGAAACTCG ACCTGGCTCC ACAGATGTTG CTGCCAGCCA GCATGTGCTA 
GTATGTGCTT TACAAGAACT TGGAAATCTA ATATATGGTC TTGGCACAAC AGCTGCACCT 
TTATTACAGG ATTCAAGTGC AGGGATTCTT GACAATGTCA TTTCAGTTAT TGTTCATCCC 
AGCATTTCAG TTCGATTAGC AGCAGCTTGG TGTTTACACT GCATTGCTGT AGCATTGCCT 
TCCTACCTAA CTCCACTTTT GGACAGTTGC CTTGAACGAC TTACTGTGCT CAAATCCTCA 
CCTGAAGCTG TGACTGGCTT TAGTTTTGCT GTGGCAGCTC TACTAGGAGC AGTGAAATAT 
TGCCCTTTAG GAATTCCACA TGGAAAAGGA AAGATAATTG TGATGTTAGC AGAGGATTTG 
TTATGTTCTG CTGCTCAGAA TAGTCGCCTT TCAGTTCAAC GAACACAAGC TGGATGGTTG 
CTGATAGCTG CCTTAATGAC ATTAGGCTCT GCATTTATTC ATCACCACCT TCCCCGGGTG 
CTAAGATTAT GGAAATGTGT TTTTCCACTA TCTCCTAAGG ATCTAGAAAC AGAAAGAAGT 
CGAGGAGACT CATTTACATG GCAGGTGACC TTGGAAGGTC GTGCAGGGGC CTTCTGTGCT 
ATCAAGAGCT TTGTTTCCCA CTGTAATGAT CTTCTTACTG ATGAAGTCAT TCAGAGACTT 
CTTCCTCCCC TTCCGTGTGC TGTTAATTTA TTAACTCAAC TTTCTTCAAT ACAGAAAACA 
TATGGAAGTC CTTTAAAAAC TCCTTCAGTG GTTTACAAAC AAAGACTTTA TGAACTATTG 
GCTTTATTAC CTCCTGAGAT CTATGCAGGA AGCCTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACCTGA CTTCTTCTGA TACCCAGATG GCAGCATCCA CTTTCTTACT TCCACCCCTC 
TGTCACCAAG ATGATCTTTT ATTACTAAGT CCTTTGCTAC AAGACACTGA CCACAGATTT 
ATTGAAGAAC AGCTCCTCCT TGGTAATGGT GTTGCTTGTG GAAGTCTAGA GTATGACCCA 
TTTTCAGTTT ATGAACATGG CATAGAAGGG GATTCAGTAC CTAAGCCCCT ACCTCCAATG 
TTGTCACTTA TCAGCTCTGC AGCCAGGCTT TTTGGAATTG TATGTGCCCA TGTAGCAGAA 
TCTCAAAGGC TCTTGGTATT GGAACAACTT TTGGACAAAA TAAAACACAC AAAAGCATCT 
CGTCAACAAG TAGTACAATT ACATGTTATG GCAGCATTTT CTAATTTTTT GAAGTATGTG 
GCCAACTCCA AAGGAAATTT AGGCTCAGAA GAAATTAGAA GACCTGCACT AACATTATTG 
ATGGGAGCCT TGGAAAGTAC CAACCCCTTA ATGCGTTGTA CTGCTGCAGA AGGTTTGGCT 
AGATTAGCCC AAGTGATTGG TGATGGCTCC TTCACTGCTG GATTAGCACA GGTTAGCTTT 
GAAAAACTGA AATCAGCTAG GGATGTGGTA ACCAGAACAG GACATTCTTT GGCTCTGGGA 
TCCTTACATA GATATTTAGG AGGAGTGGGC TCTTCTCAGC ACTTAAATGC TTGCATCGGA 
ATACTTTACA CTCTGTCACA GGATGGAACT TCTCCTGATG TACAGACATG GGCCCTACAT 
TCTCTCTCAT TGATAATTGA TTCTGCTGGT CCACTGTACC ATTTACATGT GGAATCTACC 
CTCTCTCTTA TACTAATGTT GTTGTTGACT GTTCCACCTA CTCATGCTGA AGTTCACCAA 
AGTCTTGGAC GATGTTTGAA TGTACTCATC ACCACATTGG GTCCAGAACT ACAAGGTAGC 
AGTACTGCAG TTTCTACTTT ACGGACATCC TGTCTTTTGG GGTGTGCAAT AATGCAGGAT 
AACCCAGATT GCCTTGTTCA AGCTCAAGCC ATCTCTTGTC TTCAACAACT TCATATGTTT 
GCCCCACGCT ATGTCAATTT ATCTAGTCTA GTCAGCTGTC TTTGTGTGAA TCTCTGTAGC 
TCCTACTTGT TTCTGAGAAG AGCAGTACTA GCTTGTTTAC GTCAAATTGT GCAAAGGGAA 
GCAGCTGAGG TTTCAGAACA TGCTGTTGTT CTTGCTAAGG ACAGCAGAGA AGACTTAATT 
CCAGATGCTA ATATCCGAGA AGTGGGTCTT GAGGGGGCAT TGTTGAACTT ACTAGACAAA 
GAATCTGATC AGAGATTGTG CCAGGATATC AAAGAGACCC TGAGTCATAT GATCACATCA 
ATGGCAGTGG GAAAGCTTTC TTTTTGGCTA AAACTTTGTA AAGATGTCCT TGCTTCATCA 
GCTGATTTTA CAACTGTTGC TTCTGTGGAT ACAACTCAGG ATGATGAAAT AGATAAAGAA 
GATGATGCTT CAGTCCTAAC CTACAGAAAT GATGAGAAAT GCCGGCCAAT TACAAATCCA 
CGATGGGCTA CTAGAGTTTT TGCTGCTGAA TGTGTGTGTA AAATAATCAG CCAGTGTGAG 
AATTCAGACA GTGCTCACTT TGACATTGCT TTGGCTCAAG AAATGAAAGA AAGGGATTCA 
CGAAATGATT TTTTGGTATT ACATCTTGCT GACTTGATCC GCATGGCTTT TATGGCTGCA 
ACAGACCACA GTGACCAGCT CCGTCTTTCT GGTCTTCAGA CACTGTTAGT AGTTGTTCGG 
CAATTTGCAA CTATTCCTGA ACCAGAATTC CCAGGTCATG TGATTCTGGA ACAGTATCAA 
GCCAATGTGG GAGCAGCACT TAGACCAGCT TTTACTTCAG ATACACCACC AGATGTAACT 
GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG ATAGCAAGTG GAGTTGTAAG TGATCTTAAT 
GATCTCCGAA GAGTTCATCA ATTGCTTGTA TCATCCTTGA CGAAAATACA GGCTGGAAAA 
GAAGCCTTGA GTCAATTATA TAACGAAAGT GCTTCTACTA TGGAAATCCT AGCTGTTCTC 
AAAGCCTGGG CAGAGGTTTA CATAATTGCT GTAGAAAGAC AGAAAAAACA AACTGAGAAA 
CAGAAGCAGA CTATGAAAAT TGTTGGCTCT ATAGAGGAAA ATGCCAGAGA TGTGTCATCT 
TCCATAGATG GACTTCTTGA CTTAGTCCAA ACAGATCTTG GAACATTAAG CAAACTCTGG 
CTTGCTGCTC TTCAAGATTT TGCTCTTCTA ACTTTGCCTT CAGAATTTGC TTCCCAACTT 
CCTCTTGAAG GTGGAGCTTT CTACACTGCA GAAACTAGTG AAAATGCAAA GCCACACTAT 
CATAATTCGT GGGCTCTTAT TCTTCATGCT ACAGCATTGT GGCTCACTAG CACAGGCTTC 
ATTGTAGCTG ACCCAGATGA AGGAGCAACT AATCTCTCAA GACCTGTAAC ACCGACTACA 
ATGTGTCAGG GTTCTTCTGG GGCATCAGTG AAATCCCCTG AGGATGTTTA TACAGACAGA 
TTTCACCTTA TCTTGGGAAT CAGTGTGGAA TTTCTCTGTT CCCTGCGTTC AGATGCAACC 
ATGGAAAGCA TCACTGCTTG TTTACATGCA CTGCAGGCTT TACTGGATGT TCCTTGGCCC 
AGATCTAAGA TTGGCAGTGA CCAGGACTTG GGAGTTGAGT TGTTGAATGT ACTCCATCGA 
GTAATACTGA CTAGAGAGTC ACCTTCCATT CAGTTAGCTT CACTTGAAGT TGTAAGACAG 
GTTATCTACG CAGCCCAAGA ACATGTAAAG GAAAAGAGGA GAAGTGCAGA AGTTGATGAT 
GGAGCTGCTG AAAAGGAAAC CCTGCCAGAA TTTGGAGAAG GAAAGGATAC GGGAGGACTG 
GTACCTGGAA GATCTCTGGT GTTTGCAACA TTGGAGCTAT GTGTATGCAT TTTGGTCCGG 
CAACTTCCAG AGTTAAATCC TAAACTGACC GGTAGTCCAG GAATAAAAAC TACAAAAGCA 
CAGATTCTTT CAGATGATGG AAATAAGTTG GTTTCAGCAG CATTAACTAT CCTCTCAGAA 
CTTCCTGGAG TATGCTCTCC CGAAGGAAGC ATTTCAGTTC TCCCTACTAT TCTGTACCTT 
ATAATTGGGG TCCTCCGAGA AACTGCTGTG AGGTTACCTG GAGGACAGTT ATCTTATACT 
GTTGGAGCTG CTCTACAAGC CTTGAAAGGA ATATTGGCAT CTCCCTTGGC TCGAGCAGAG 
AAGAGTCACA CTGCTTGGAC TGACCTTCTT CGAAGTGCTT TGACAACAAT CCTAGACTGC 
TGGGATCCAG ATGGAACACA GCAAGAGTTT GATGAAATCA GCCTGCTTAC TGCCATAACA 
GTATTTATCT TGTCTTCAAG TCCAGAAGTG ACCACCATCC AGTGCCTGCA AGAACGTTGT 
ATACAGAAGT TTAAAGCTAC TCTTGAGTTA AAAGACCCAG TAGTACAAAT AAAGTGCTAT 
CAGCTACTGC TTTCCATTTT CCAGTATCCA AACCCAGCAG TATCCTACCC TTATATTCAT 
TCATTAGCAT CATCTATTGT GAGAAAACTG CAAGAGACAG ATAAAGTAAA ACCAGAAGAT 
GCTGCTGAGC TTCAGGTGAT TCAAGAAGGT ATTAAGGTCT TAGAAGCCCT AGTAGGGATT 
GCGGAAGAAC AGCACCGTGC TCACTTGGTG GCCGACATTC TCCCTATTCT TATTTCCTTT 
CTTTTGGATG AAAACACTCT GGGCTCAGCA CCCACCACAA TGAGAAATTT ACATGACTTT 
GCTTTGCAGA ATCTGATGCA GATTGGTCCC CAGTATTCAT CAACTTTTAA AAATATTATG 
GCTTCTTCTC CCACAATGAA AGCCCGTCTT GAGGCAGCTA TAAAAGGCAA TCAGGAAAAT 
ATCAAAATCA AGACAACCCC AAAACATATC ACAAAACCTG GAAAAAATTC AAGTATTCAG 
TTGAAGACCA ATTTTTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_023501135.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023356903.1 Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X5, mRNA.

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CloneID	OSa117582
Clone ID Related Accession (Same CDS sequence)	XM_023501135.1 , XM_023501135.2 , XM_012546981.2 , XM_012546981.1
Accession Version	XM_023501135.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 5A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_023501135.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG CTCACAGCTT ATTGCTGAAT GAAGAAGCAT ATAATCAACT TGGAGAAGTT 
CAGAAGGCAG AATTTGTTTT TGAATGGTTG AAATTTTTAG AGAAACTTTT GCTAGCAACC 
AACAAGGCTG ACATAAAGGA GAAACAGAAG AGTATTATTG AGCAGCTCAT ATCTCTATTA 
AATAGCTCTC CAGGGCCACC TACCCGAAAG CTTCTTGCTG AGAACCTAGC TGTTCTTTAT 
AGTATTGGAG ATACATTTTC TGTTTATCAA ACAATTGACA AATGTAATGA ACTCATTCGT 
AGCAAAGATG ACTCTCCAAG TTATCTTCCC ACAAAACTTG CTGCTGTGGT ATGTCTGGGT 
TCTTTGTATA AGAAGTTGGG GAGACTATTG GGTAACACCT TTATAGATAC AGTGGGGAAT 
ATACTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGTCGAT ATGAGATTAT GCTGAGTTTG 
CAAAATATAC TGAGTGGACT TGGAGCTGCT GCTGCCCCTT GTCACAGAGA CATTTATAAA 
GCTGCAAGGT CATGTTTGAC AGACAGGTCC ATGGCAGTTC GCTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAGAATGA AGCTAGCTTT ATGTGGAGTA CAGACATAGA CAGTGTTGCA 
ACTTTGTGTT TTAAGTCATT TGAAGGTTCC AACTATGATG TGCGAATAGC AGTATCAAAG 
CTCTTAGGTA CAGTGTTGGC TAGAGCTGTA ATGTCCAAAC CTGGAACAGC AACTTCCCGG 
CAAAATGTTC GCAAAGTTTC TCTGGAGGAA GCCATGGAGT TACTAGGAAC AGGGTTTCTA 
CGTGGGAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGT TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG TGTTACTCAG GCATATGTGG TGTTTATTTC AACACTAGGA 
GGACGTTGGT TAGAAAGAAA TTTTTCCATC TTACTCTCTC ATATCCTTAG CCTAGTATCA 
CAGTTTCACC CTAAAGCAAT CCAAACTCAG ATGGATGCTA TCTGTTGTCG TCGCTGTGTT 
TCATTTATCC TTCGAACTAC TATAGGAAGC CTTCTCGGGG AAAAAGCTCA AATTGCTGCT 
GCCAGGGATA TTTGTCAGGT CATCTGGAAA CTAAAGAAAA CTGTTGATGC TGTAATGAGT 
GAAGTTAATT TGGAAACTCG ACCTGGCTCC ACAGATGTTG CTGCCAGCCA GCATGTGCTA 
GTATGTGCTT TACAAGAACT TGGAAATCTA ATATATGGTC TTGGCACAAC AGCTGCACCT 
TTATTACAGG ATTCAAGTGC AGGGATTCTT GACAATGTCA TTTCAGTTAT TGTTCATCCC 
AGCATTTCAG TTCGATTAGC AGCAGCTTGG TGTTTACACT GCATTGCTGT AGCATTGCCT 
TCCTACCTAA CTCCACTTTT GGACAGTTGC CTTGAACGAC TTACTGTGCT CAAATCCTCA 
CCTGAAGCTG TGACTGGCTT TAGTTTTGCT GTGGCAGCTC TACTAGGAGC AGTGAAATAT 
TGCCCTTTAG GAATTCCACA TGGAAAAGGA AAGATAATTG TGATGTTAGC AGAGGATTTG 
TTATGTTCTG CTGCTCAGAA TAGTCGCCTT TCAGTTCAAC GAACACAAGC TGGATGGTTG 
CTGATAGCTG CCTTAATGAC ATTAGGCTCT GCATTTATTC ATCACCACCT TCCCCGGGTG 
CTAAGATTAT GGAAATGTGT TTTTCCACTA TCTCCTAAGG ATCTAGAAAC AGAAAGAAGT 
CGAGGAGACT CATTTACATG GCAGGTGACC TTGGAAGGTC GTGCAGGGGC CTTCTGTGCT 
ATCAAGAGCT TTGTTTCCCA CTGTAATGAT CTTCTTACTG ATGAAGTCAT TCAGAGACTT 
CTTCCTCCCC TTCCGTGTGC TGTTAATTTA TTAACTCAAC TTTCTTCAAT ACAGAAAACA 
TATGGAAGTC CTTTAAAAAC TCCTTCAGTG GTTTACAAAC AAAGACTTTA TGAACTATTG 
GCTTTATTAC CTCCTGAGAT CTATGCAGGA AGCCTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACCTGA CTTCTTCTGA TACCCAGATG GCAGCATCCA CTTTCTTACT TCCACCCCTC 
TGTCACCAAG ATGATCTTTT ATTACTAAGT CCTTTGCTAC AAGACACTGA CCACAGATTT 
ATTGAAGAAC AGCTCCTCCT TGGTAATGGT GTTGCTTGTG GAAGTCTAGA GTATGACCCA 
TTTTCAGTTT ATGAACATGG CATAGAAGGG GATTCAGTAC CTAAGCCCCT ACCTCCAATG 
TTGTCACTTA TCAGCTCTGC AGCCAGGCTT TTTGGAATTG TATGTGCCCA TGTAGCAGAA 
TCTCAAAGGC TCTTGGTATT GGAACAACTT TTGGACAAAA TAAAACACAC AAAAGCATCT 
CGTCAACAAG TAGTACAATT ACATGTTATG GCAGCATTTT CTAATTTTTT GAAGTATGTG 
GCCAACTCCA AAGGAAATTT AGGCTCAGAA GAAATTAGAA GACCTGCACT AACATTATTG 
ATGGGAGCCT TGGAAAGTAC CAACCCCTTA ATGCGTTGTA CTGCTGCAGA AGGTTTGGCT 
AGATTAGCCC AAGTGATTGG TGATGGCTCC TTCACTGCTG GATTAGCACA GGTTAGCTTT 
GAAAAACTGA AATCAGCTAG GGATGTGGTA ACCAGAACAG GACATTCTTT GGCTCTGGGA 
TCCTTACATA GATATTTAGG AGGAGTGGGC TCTTCTCAGC ACTTAAATGC TTGCATCGGA 
ATACTTTACA CTCTGTCACA GGATGGAACT TCTCCTGATG TACAGACATG GGCCCTACAT 
TCTCTCTCAT TGATAATTGA TTCTGCTGGT CCACTGTACC ATTTACATGT GGAATCTACC 
CTCTCTCTTA TACTAATGTT GTTGTTGACT GTTCCACCTA CTCATGCTGA AGTTCACCAA 
AGTCTTGGAC GATGTTTGAA TGTACTCATC ACCACATTGG GTCCAGAACT ACAAGGTAGC 
AGTACTGCAG TTTCTACTTT ACGGACATCC TGTCTTTTGG GGTGTGCAAT AATGCAGGAT 
AACCCAGATT GCCTTGTTCA AGCTCAAGCC ATCTCTTGTC TTCAACAACT TCATATGTTT 
GCCCCACGCT ATGTCAATTT ATCTAGTCTA GTCAGCTGTC TTTGTGTGAA TCTCTGTAGC 
TCCTACTTGT TTCTGAGAAG AGCAGTACTA GCTTGTTTAC GTCAAATTGT GCAAAGGGAA 
GCAGCTGAGG TTTCAGAACA TGCTGTTGTT CTTGCTAAGG ACAGCAGAGA AGACTTAATT 
CCAGATGCTA ATATCCGAGA AGTGGGTCTT GAGGGGGCAT TGTTGAACTT ACTAGACAAA 
GAATCTGATC AGAGATTGTG CCAGGATATC AAAGAGACCC TGAGTCATAT GATCACATCA 
ATGGCAGTGG GAAAGCTTTC TTTTTGGCTA AAACTTTGTA AAGATGTCCT TGCTTCATCA 
GCTGATTTTA CAACTGTTGC TTCTGTGGAT ACAACTCAGG ATGATGAAAT AGATAAAGAA 
GATGATGCTT CAGTCCTAAC CTACAGAAAT GATGAGAAAT GCCGGCCAAT TACAAATCCA 
CGATGGGCTA CTAGAGTTTT TGCTGCTGAA TGTGTGTGTA AAATAATCAG CCAGTGTGAG 
AATTCAGACA GTGCTCACTT TGACATTGCT TTGGCTCAAG AAATGAAAGA AAGGGATTCA 
CGAAATGATT TTTTGGTATT ACATCTTGCT GACTTGATCC GCATGGCTTT TATGGCTGCA 
ACAGACCACA GTGACCAGCT CCGTCTTTCT GGTCTTCAGA CACTGTTAGT AGTTGTTCGG 
CAATTTGCAA CTATTCCTGA ACCAGAATTC CCAGGTCATG TGATTCTGGA ACAGTATCAA 
GCCAATGTGG GAGCAGCACT TAGACCAGCT TTTACTTCAG ATACACCACC AGATGTAACT 
GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG ATAGCAAGTG GAGTTGTAAG TGATCTTAAT 
GATCTCCGAA GAGTTCATCA ATTGCTTGTA TCATCCTTGA CGAAAATACA GGCTGGAAAA 
GAAGCCTTGA GTCAATTATA TAACGAAAGT GCTTCTACTA TGGAAATCCT AGCTGTTCTC 
AAAGCCTGGG CAGAGGTTTA CATAATTGCT GTAGAAAGAC AGAAAAAACA AACTGAGAAA 
CAGAAGCAGA CTATGAAAAT TGTTGGCTCT ATAGAGGAAA ATGCCAGAGA TGTGTCATCT 
TCCATAGATG GACTTCTTGA CTTAGTCCAA ACAGATCTTG GAACATTAAG CAAACTCTGG 
CTTGCTGCTC TTCAAGATTT TGCTCTTCTA ACTTTGCCTT CAGAATTTGC TTCCCAACTT 
CCTCTTGAAG GTGGAGCTTT CTACACTGCA GAAACTAGTG AAAATGCAAA GCCACACTAT 
CATAATTCGT GGGCTCTTAT TCTTCATGCT ACAGCATTGT GGCTCACTAG CACAGGCTTC 
ATTGTAGCTG ACCCAGATGA AGGAGCAACT AATCTCTCAA GACCTGTAAC ACCGACTACA 
ATGTGTCAGG GTTCTTCTGG GGCATCAGTG AAATCCCCTG AGGATGTTTA TACAGACAGA 
TTTCACCTTA TCTTGGGAAT CAGTGTGGAA TTTCTCTGTT CCCTGCGTTC AGATGCAACC 
ATGGAAAGCA TCACTGCTTG TTTACATGCA CTGCAGGCTT TACTGGATGT TCCTTGGCCC 
AGATCTAAGA TTGGCAGTGA CCAGGACTTG GGAGTTGAGT TGTTGAATGT ACTCCATCGA 
GTAATACTGA CTAGAGAGTC ACCTTCCATT CAGTTAGCTT CACTTGAAGT TGTAAGACAG 
GTTATCTACG CAGCCCAAGA ACATGTAAAG GAAAAGAGGA GAAGTGCAGA AGTTGATGAT 
GGAGCTGCTG AAAAGGAAAC CCTGCCAGAA TTTGGAGAAG GAAAGGATAC GGGAGGACTG 
GTACCTGGAA GATCTCTGGT GTTTGCAACA TTGGAGCTAT GTGTATGCAT TTTGGTCCGG 
CAACTTCCAG AGTTAAATCC TAAACTGACC GGTAGTCCAG GAATAAAAAC TACAAAAGCA 
CAGATTCTTT CAGATGATGG AAATAAGTTG GTTTCAGCAG CATTAACTAT CCTCTCAGAA 
CTTCCTGGAG TATGCTCTCC CGAAGGAAGC ATTTCAGTTC TCCCTACTAT TCTGTACCTT 
ATAATTGGGG TCCTCCGAGA AACTGCTGTG AGGTTACCTG GAGGACAGTT ATCTTATACT 
GTTGGAGCTG CTCTACAAGC CTTGAAAGGA ATATTGGCAT CTCCCTTGGC TCGAGCAGAG 
AAGAGTCACA CTGCTTGGAC TGACCTTCTT CGAAGTGCTT TGACAACAAT CCTAGACTGC 
TGGGATCCAG ATGGAACACA GCAAGAGTTT GATGAAATCA GCCTGCTTAC TGCCATAACA 
GTATTTATCT TGTCTTCAAG TCCAGAAGTG ACCACCATCC AGTGCCTGCA AGAACGTTGT 
ATACAGAAGT TTAAAGCTAC TCTTGAGTTA AAAGACCCAG TAGTACAAAT AAAGTGCTAT 
CAGCTACTGC TTTCCATTTT CCAGTATCCA AACCCAGCAG TATCCTACCC TTATATTCAT 
TCATTAGCAT CATCTATTGT GAGAAAACTG CAAGAGACAG ATAAAGTAAA ACCAGAAGAT 
GCTGCTGAGC TTCAGGTGAT TCAAGAAGGT ATTAAGGTCT TAGAAGCCCT AGTAGGGATT 
GCGGAAGAAC AGCACCGTGC TCACTTGGTG GCCGACATTC TCCCTATTCT TATTTCCTTT 
CTTTTGGATG AAAACACTCT GGGCTCAGCA CCCACCACAA TGAGAAATTT ACATGACTTT 
GCTTTGCAGA ATCTGATGCA GATTGGTCCC CAGTATTCAT CAACTTTTAA AAATATTATG 
GCTTCTTCTC CCACAATGAA AGCCCGTCTT GAGGCAGCTA TAAAAGGCAA TCAGGAAAAT 
ATCAAAATCA AGACAACCCC AAAACATATC ACAAAACCTG GAAAAAATTC AAGTATTCAG 
TTGAAGACCA ATTTTTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023356903.1
CDS	284..6424
Translation	MELAHSLLLNEEAYNQLGEVQKAEFVFEWLKFLEKLLLATNKADIKEKQKSIIEQLISLLNSSPGPPTRKLLAENLAVLYSIGDTFSVYQTIDKCNELIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRLLGNTFIDTVGNILKAMKSAESQGRYEIMLSLQNILSGLGAAAAPCHRDIYKAARSCLTDRSMAVRCAAAKCLLELQNEASFMWSTDIDSVATLCFKSFEGSNYDVRIAVSKLLGTVLARAVMSKPGTATSRQNVRKVSLEEAMELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFISTLGGRWLERNFSILLSHILSLVSQFHPKAIQTQMDAICCRRCVSFILRTTIGSLLGEKAQIAAARDICQVIWKLKKTVDAVMSEVNLETRPGSTDVAASQHVLVCALQELGNLIYGLGTTAAPLLQDSSAGILDNVISVIVHPSISVRLAAAWCLHCIAVALPSYLTPLLDSCLERLTVLKSSPEAVTGFSFAVAALLGAVKYCPLGIPHGKGKIIVMLAEDLLCSAAQNSRLSVQRTQAGWLLIAALMTLGSAFIHHHLPRVLRLWKCVFPLSPKDLETERSRGDSFTWQVTLEGRAGAFCAIKSFVSHCNDLLTDEVIQRLLPPLPCAVNLLTQLSSIQKTYGSPLKTPSVVYKQRLYELLALLPPEIYAGSLCAILKELAADLTSSDTQMAASTFLLPPLCHQDDLLLLSPLLQDTDHRFIEEQLLLGNGVACGSLEYDPFSVYEHGIEGDSVPKPLPPMLSLISSAARLFGIVCAHVAESQRLLVLEQLLDKIKHTKASRQQVVQLHVMAAFSNFLKYVANSKGNLGSEEIRRPALTLLMGALESTNPLMRCTAAEGLARLAQVIGDGSFTAGLAQVSFEKLKSARDVVTRTGHSLALGSLHRYLGGVGSSQHLNACIGILYTLSQDGTSPDVQTWALHSLSLIIDSAGPLYHLHVESTLSLILMLLLTVPPTHAEVHQSLGRCLNVLITTLGPELQGSSTAVSTLRTSCLLGCAIMQDNPDCLVQAQAISCLQQLHMFAPRYVNLSSLVSCLCVNLCSSYLFLRRAVLACLRQIVQREAAEVSEHAVVLAKDSREDLIPDANIREVGLEGALLNLLDKESDQRLCQDIKETLSHMITSMAVGKLSFWLKLCKDVLASSADFTTVASVDTTQDDEIDKEDDASVLTYRNDEKCRPITNPRWATRVFAAECVCKIISQCENSDSAHFDIALAQEMKERDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLQTLLVVVRQFATIPEPEFPGHVILEQYQANVGAALRPAFTSDTPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSQLYNESASTMEILAVLKAWAEVYIIAVERQKKQTEKQKQTMKIVGSIEENARDVSSSIDGLLDLVQTDLGTLSKLWLAALQDFALLTLPSEFASQLPLEGGAFYTAETSENAKPHYHNSWALILHATALWLTSTGFIVADPDEGATNLSRPVTPTTMCQGSSGASVKSPEDVYTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGVELLNVLHRVILTRESPSIQLASLEVVRQVIYAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGRSLVFATLELCVCILVRQLPELNPKLTGSPGIKTTKAQILSDDGNKLVSAALTILSELPGVCSPEGSISVLPTILYLIIGVLRETAVRLPGGQLSYTVGAALQALKGILASPLARAEKSHTAWTDLLRSALTTILDCWDPDGTQQEFDEISLLTAITVFILSSSPEVTTIQCLQERCIQKFKATLELKDPVVQIKCYQLLLSIFQYPNPAVSYPYIHSLASSIVRKLQETDKVKPEDAAELQVIQEGIKVLEALVGIAEEQHRAHLVADILPILISFLLDENTLGSAPTTMRNLHDFALQNLMQIGPQYSSTFKNIMASSPTMKARLEAAIKGNQENIKIKTTPKHITKPGKNSSIQLKTNFF

Target ORF information:

RefSeq Version	XM_023501135.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023501135.2

ORF Insert Sequence:

ATGGAACTGG CTCACAGCTT ATTGCTGAAT GAAGAAGCAT ATAATCAACT TGGAGAAGTT 
CAGAAGGCAG AATTTGTTTT TGAATGGTTG AAATTTTTAG AGAAACTTTT GCTAGCAACC 
AACAAGGCTG ACATAAAGGA GAAACAGAAG AGTATTATTG AGCAGCTCAT ATCTCTATTA 
AATAGCTCTC CAGGGCCACC TACCCGAAAG CTTCTTGCTG AGAACCTAGC TGTTCTTTAT 
AGTATTGGAG ATACATTTTC TGTTTATCAA ACAATTGACA AATGTAATGA ACTCATTCGT 
AGCAAAGATG ACTCTCCAAG TTATCTTCCC ACAAAACTTG CTGCTGTGGT ATGTCTGGGT 
TCTTTGTATA AGAAGTTGGG GAGACTATTG GGTAACACCT TTATAGATAC AGTGGGGAAT 
ATACTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGTCGAT ATGAGATTAT GCTGAGTTTG 
CAAAATATAC TGAGTGGACT TGGAGCTGCT GCTGCCCCTT GTCACAGAGA CATTTATAAA 
GCTGCAAGGT CATGTTTGAC AGACAGGTCC ATGGCAGTTC GCTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAGAATGA AGCTAGCTTT ATGTGGAGTA CAGACATAGA CAGTGTTGCA 
ACTTTGTGTT TTAAGTCATT TGAAGGTTCC AACTATGATG TGCGAATAGC AGTATCAAAG 
CTCTTAGGTA CAGTGTTGGC TAGAGCTGTA ATGTCCAAAC CTGGAACAGC AACTTCCCGG 
CAAAATGTTC GCAAAGTTTC TCTGGAGGAA GCCATGGAGT TACTAGGAAC AGGGTTTCTA 
CGTGGGAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGT TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG TGTTACTCAG GCATATGTGG TGTTTATTTC AACACTAGGA 
GGACGTTGGT TAGAAAGAAA TTTTTCCATC TTACTCTCTC ATATCCTTAG CCTAGTATCA 
CAGTTTCACC CTAAAGCAAT CCAAACTCAG ATGGATGCTA TCTGTTGTCG TCGCTGTGTT 
TCATTTATCC TTCGAACTAC TATAGGAAGC CTTCTCGGGG AAAAAGCTCA AATTGCTGCT 
GCCAGGGATA TTTGTCAGGT CATCTGGAAA CTAAAGAAAA CTGTTGATGC TGTAATGAGT 
GAAGTTAATT TGGAAACTCG ACCTGGCTCC ACAGATGTTG CTGCCAGCCA GCATGTGCTA 
GTATGTGCTT TACAAGAACT TGGAAATCTA ATATATGGTC TTGGCACAAC AGCTGCACCT 
TTATTACAGG ATTCAAGTGC AGGGATTCTT GACAATGTCA TTTCAGTTAT TGTTCATCCC 
AGCATTTCAG TTCGATTAGC AGCAGCTTGG TGTTTACACT GCATTGCTGT AGCATTGCCT 
TCCTACCTAA CTCCACTTTT GGACAGTTGC CTTGAACGAC TTACTGTGCT CAAATCCTCA 
CCTGAAGCTG TGACTGGCTT TAGTTTTGCT GTGGCAGCTC TACTAGGAGC AGTGAAATAT 
TGCCCTTTAG GAATTCCACA TGGAAAAGGA AAGATAATTG TGATGTTAGC AGAGGATTTG 
TTATGTTCTG CTGCTCAGAA TAGTCGCCTT TCAGTTCAAC GAACACAAGC TGGATGGTTG 
CTGATAGCTG CCTTAATGAC ATTAGGCTCT GCATTTATTC ATCACCACCT TCCCCGGGTG 
CTAAGATTAT GGAAATGTGT TTTTCCACTA TCTCCTAAGG ATCTAGAAAC AGAAAGAAGT 
CGAGGAGACT CATTTACATG GCAGGTGACC TTGGAAGGTC GTGCAGGGGC CTTCTGTGCT 
ATCAAGAGCT TTGTTTCCCA CTGTAATGAT CTTCTTACTG ATGAAGTCAT TCAGAGACTT 
CTTCCTCCCC TTCCGTGTGC TGTTAATTTA TTAACTCAAC TTTCTTCAAT ACAGAAAACA 
TATGGAAGTC CTTTAAAAAC TCCTTCAGTG GTTTACAAAC AAAGACTTTA TGAACTATTG 
GCTTTATTAC CTCCTGAGAT CTATGCAGGA AGCCTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACCTGA CTTCTTCTGA TACCCAGATG GCAGCATCCA CTTTCTTACT TCCACCCCTC 
TGTCACCAAG ATGATCTTTT ATTACTAAGT CCTTTGCTAC AAGACACTGA CCACAGATTT 
ATTGAAGAAC AGCTCCTCCT TGGTAATGGT GTTGCTTGTG GAAGTCTAGA GTATGACCCA 
TTTTCAGTTT ATGAACATGG CATAGAAGGG GATTCAGTAC CTAAGCCCCT ACCTCCAATG 
TTGTCACTTA TCAGCTCTGC AGCCAGGCTT TTTGGAATTG TATGTGCCCA TGTAGCAGAA 
TCTCAAAGGC TCTTGGTATT GGAACAACTT TTGGACAAAA TAAAACACAC AAAAGCATCT 
CGTCAACAAG TAGTACAATT ACATGTTATG GCAGCATTTT CTAATTTTTT GAAGTATGTG 
GCCAACTCCA AAGGAAATTT AGGCTCAGAA GAAATTAGAA GACCTGCACT AACATTATTG 
ATGGGAGCCT TGGAAAGTAC CAACCCCTTA ATGCGTTGTA CTGCTGCAGA AGGTTTGGCT 
AGATTAGCCC AAGTGATTGG TGATGGCTCC TTCACTGCTG GATTAGCACA GGTTAGCTTT 
GAAAAACTGA AATCAGCTAG GGATGTGGTA ACCAGAACAG GACATTCTTT GGCTCTGGGA 
TCCTTACATA GATATTTAGG AGGAGTGGGC TCTTCTCAGC ACTTAAATGC TTGCATCGGA 
ATACTTTACA CTCTGTCACA GGATGGAACT TCTCCTGATG TACAGACATG GGCCCTACAT 
TCTCTCTCAT TGATAATTGA TTCTGCTGGT CCACTGTACC ATTTACATGT GGAATCTACC 
CTCTCTCTTA TACTAATGTT GTTGTTGACT GTTCCACCTA CTCATGCTGA AGTTCACCAA 
AGTCTTGGAC GATGTTTGAA TGTACTCATC ACCACATTGG GTCCAGAACT ACAAGGTAGC 
AGTACTGCAG TTTCTACTTT ACGGACATCC TGTCTTTTGG GGTGTGCAAT AATGCAGGAT 
AACCCAGATT GCCTTGTTCA AGCTCAAGCC ATCTCTTGTC TTCAACAACT TCATATGTTT 
GCCCCACGCT ATGTCAATTT ATCTAGTCTA GTCAGCTGTC TTTGTGTGAA TCTCTGTAGC 
TCCTACTTGT TTCTGAGAAG AGCAGTACTA GCTTGTTTAC GTCAAATTGT GCAAAGGGAA 
GCAGCTGAGG TTTCAGAACA TGCTGTTGTT CTTGCTAAGG ACAGCAGAGA AGACTTAATT 
CCAGATGCTA ATATCCGAGA AGTGGGTCTT GAGGGGGCAT TGTTGAACTT ACTAGACAAA 
GAATCTGATC AGAGATTGTG CCAGGATATC AAAGAGACCC TGAGTCATAT GATCACATCA 
ATGGCAGTGG GAAAGCTTTC TTTTTGGCTA AAACTTTGTA AAGATGTCCT TGCTTCATCA 
GCTGATTTTA CAACTGTTGC TTCTGTGGAT ACAACTCAGG ATGATGAAAT AGATAAAGAA 
GATGATGCTT CAGTCCTAAC CTACAGAAAT GATGAGAAAT GCCGGCCAAT TACAAATCCA 
CGATGGGCTA CTAGAGTTTT TGCTGCTGAA TGTGTGTGTA AAATAATCAG CCAGTGTGAG 
AATTCAGACA GTGCTCACTT TGACATTGCT TTGGCTCAAG AAATGAAAGA AAGGGATTCA 
CGAAATGATT TTTTGGTATT ACATCTTGCT GACTTGATCC GCATGGCTTT TATGGCTGCA 
ACAGACCACA GTGACCAGCT CCGTCTTTCT GGTCTTCAGA CACTGTTAGT AGTTGTTCGG 
CAATTTGCAA CTATTCCTGA ACCAGAATTC CCAGGTCATG TGATTCTGGA ACAGTATCAA 
GCCAATGTGG GAGCAGCACT TAGACCAGCT TTTACTTCAG ATACACCACC AGATGTAACT 
GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG ATAGCAAGTG GAGTTGTAAG TGATCTTAAT 
GATCTCCGAA GAGTTCATCA ATTGCTTGTA TCATCCTTGA CGAAAATACA GGCTGGAAAA 
GAAGCCTTGA GTCAATTATA TAACGAAAGT GCTTCTACTA TGGAAATCCT AGCTGTTCTC 
AAAGCCTGGG CAGAGGTTTA CATAATTGCT GTAGAAAGAC AGAAAAAACA AACTGAGAAA 
CAGAAGCAGA CTATGAAAAT TGTTGGCTCT ATAGAGGAAA ATGCCAGAGA TGTGTCATCT 
TCCATAGATG GACTTCTTGA CTTAGTCCAA ACAGATCTTG GAACATTAAG CAAACTCTGG 
CTTGCTGCTC TTCAAGATTT TGCTCTTCTA ACTTTGCCTT CAGAATTTGC TTCCCAACTT 
CCTCTTGAAG GTGGAGCTTT CTACACTGCA GAAACTAGTG AAAATGCAAA GCCACACTAT 
CATAATTCGT GGGCTCTTAT TCTTCATGCT ACAGCATTGT GGCTCACTAG CACAGGCTTC 
ATTGTAGCTG ACCCAGATGA AGGAGCAACT AATCTCTCAA GACCTGTAAC ACCGACTACA 
ATGTGTCAGG GTTCTTCTGG GGCATCAGTG AAATCCCCTG AGGATGTTTA TACAGACAGA 
TTTCACCTTA TCTTGGGAAT CAGTGTGGAA TTTCTCTGTT CCCTGCGTTC AGATGCAACC 
ATGGAAAGCA TCACTGCTTG TTTACATGCA CTGCAGGCTT TACTGGATGT TCCTTGGCCC 
AGATCTAAGA TTGGCAGTGA CCAGGACTTG GGAGTTGAGT TGTTGAATGT ACTCCATCGA 
GTAATACTGA CTAGAGAGTC ACCTTCCATT CAGTTAGCTT CACTTGAAGT TGTAAGACAG 
GTTATCTACG CAGCCCAAGA ACATGTAAAG GAAAAGAGGA GAAGTGCAGA AGTTGATGAT 
GGAGCTGCTG AAAAGGAAAC CCTGCCAGAA TTTGGAGAAG GAAAGGATAC GGGAGGACTG 
GTACCTGGAA GATCTCTGGT GTTTGCAACA TTGGAGCTAT GTGTATGCAT TTTGGTCCGG 
CAACTTCCAG AGTTAAATCC TAAACTGACC GGTAGTCCAG GAATAAAAAC TACAAAAGCA 
CAGATTCTTT CAGATGATGG AAATAAGTTG GTTTCAGCAG CATTAACTAT CCTCTCAGAA 
CTTCCTGGAG TATGCTCTCC CGAAGGAAGC ATTTCAGTTC TCCCTACTAT TCTGTACCTT 
ATAATTGGGG TCCTCCGAGA AACTGCTGTG AGGTTACCTG GAGGACAGTT ATCTTATACT 
GTTGGAGCTG CTCTACAAGC CTTGAAAGGA ATATTGGCAT CTCCCTTGGC TCGAGCAGAG 
AAGAGTCACA CTGCTTGGAC TGACCTTCTT CGAAGTGCTT TGACAACAAT CCTAGACTGC 
TGGGATCCAG ATGGAACACA GCAAGAGTTT GATGAAATCA GCCTGCTTAC TGCCATAACA 
GTATTTATCT TGTCTTCAAG TCCAGAAGTG ACCACCATCC AGTGCCTGCA AGAACGTTGT 
ATACAGAAGT TTAAAGCTAC TCTTGAGTTA AAAGACCCAG TAGTACAAAT AAAGTGCTAT 
CAGCTACTGC TTTCCATTTT CCAGTATCCA AACCCAGCAG TATCCTACCC TTATATTCAT 
TCATTAGCAT CATCTATTGT GAGAAAACTG CAAGAGACAG ATAAAGTAAA ACCAGAAGAT 
GCTGCTGAGC TTCAGGTGAT TCAAGAAGGT ATTAAGGTCT TAGAAGCCCT AGTAGGGATT 
GCGGAAGAAC AGCACCGTGC TCACTTGGTG GCCGACATTC TCCCTATTCT TATTTCCTTT 
CTTTTGGATG AAAACACTCT GGGCTCAGCA CCCACCACAA TGAGAAATTT ACATGACTTT 
GCTTTGCAGA ATCTGATGCA GATTGGTCCC CAGTATTCAT CAACTTTTAA AAATATTATG 
GCTTCTTCTC CCACAATGAA AGCCCGTCTT GAGGCAGCTA TAAAAGGCAA TCAGGAAAAT 
ATCAAAATCA AGACAACCCC AAAACATATC ACAAAACCTG GAAAAAATTC AAGTATTCAG 
TTGAAGACCA ATTTTTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_012546981.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012402435.1 Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X1, mRNA.

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CloneID	OSa117582
Clone ID Related Accession (Same CDS sequence)	XM_023501135.1 , XM_023501135.2 , XM_012546981.2 , XM_012546981.1
Accession Version	XM_012546981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 5A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816524.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012546981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG CTCACAGCTT ATTGCTGAAT GAAGAAGCAT ATAATCAACT TGGAGAAGTT 
CAGAAGGCAG AATTTGTTTT TGAATGGTTG AAATTTTTAG AGAAACTTTT GCTAGCAACC 
AACAAGGCTG ACATAAAGGA GAAACAGAAG AGTATTATTG AGCAGCTCAT ATCTCTATTA 
AATAGCTCTC CAGGGCCACC TACCCGAAAG CTTCTTGCTG AGAACCTAGC TGTTCTTTAT 
AGTATTGGAG ATACATTTTC TGTTTATCAA ACAATTGACA AATGTAATGA ACTCATTCGT 
AGCAAAGATG ACTCTCCAAG TTATCTTCCC ACAAAACTTG CTGCTGTGGT ATGTCTGGGT 
TCTTTGTATA AGAAGTTGGG GAGACTATTG GGTAACACCT TTATAGATAC AGTGGGGAAT 
ATACTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGTCGAT ATGAGATTAT GCTGAGTTTG 
CAAAATATAC TGAGTGGACT TGGAGCTGCT GCTGCCCCTT GTCACAGAGA CATTTATAAA 
GCTGCAAGGT CATGTTTGAC AGACAGGTCC ATGGCAGTTC GCTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAGAATGA AGCTAGCTTT ATGTGGAGTA CAGACATAGA CAGTGTTGCA 
ACTTTGTGTT TTAAGTCATT TGAAGGTTCC AACTATGATG TGCGAATAGC AGTATCAAAG 
CTCTTAGGTA CAGTGTTGGC TAGAGCTGTA ATGTCCAAAC CTGGAACAGC AACTTCCCGG 
CAAAATGTTC GCAAAGTTTC TCTGGAGGAA GCCATGGAGT TACTAGGAAC AGGGTTTCTA 
CGTGGGAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGT TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG TGTTACTCAG GCATATGTGG TGTTTATTTC AACACTAGGA 
GGACGTTGGT TAGAAAGAAA TTTTTCCATC TTACTCTCTC ATATCCTTAG CCTAGTATCA 
CAGTTTCACC CTAAAGCAAT CCAAACTCAG ATGGATGCTA TCTGTTGTCG TCGCTGTGTT 
TCATTTATCC TTCGAACTAC TATAGGAAGC CTTCTCGGGG AAAAAGCTCA AATTGCTGCT 
GCCAGGGATA TTTGTCAGGT CATCTGGAAA CTAAAGAAAA CTGTTGATGC TGTAATGAGT 
GAAGTTAATT TGGAAACTCG ACCTGGCTCC ACAGATGTTG CTGCCAGCCA GCATGTGCTA 
GTATGTGCTT TACAAGAACT TGGAAATCTA ATATATGGTC TTGGCACAAC AGCTGCACCT 
TTATTACAGG ATTCAAGTGC AGGGATTCTT GACAATGTCA TTTCAGTTAT TGTTCATCCC 
AGCATTTCAG TTCGATTAGC AGCAGCTTGG TGTTTACACT GCATTGCTGT AGCATTGCCT 
TCCTACCTAA CTCCACTTTT GGACAGTTGC CTTGAACGAC TTACTGTGCT CAAATCCTCA 
CCTGAAGCTG TGACTGGCTT TAGTTTTGCT GTGGCAGCTC TACTAGGAGC AGTGAAATAT 
TGCCCTTTAG GAATTCCACA TGGAAAAGGA AAGATAATTG TGATGTTAGC AGAGGATTTG 
TTATGTTCTG CTGCTCAGAA TAGTCGCCTT TCAGTTCAAC GAACACAAGC TGGATGGTTG 
CTGATAGCTG CCTTAATGAC ATTAGGCTCT GCATTTATTC ATCACCACCT TCCCCGGGTG 
CTAAGATTAT GGAAATGTGT TTTTCCACTA TCTCCTAAGG ATCTAGAAAC AGAAAGAAGT 
CGAGGAGACT CATTTACATG GCAGGTGACC TTGGAAGGTC GTGCAGGGGC CTTCTGTGCT 
ATCAAGAGCT TTGTTTCCCA CTGTAATGAT CTTCTTACTG ATGAAGTCAT TCAGAGACTT 
CTTCCTCCCC TTCCGTGTGC TGTTAATTTA TTAACTCAAC TTTCTTCAAT ACAGAAAACA 
TATGGAAGTC CTTTAAAAAC TCCTTCAGTG GTTTACAAAC AAAGACTTTA TGAACTATTG 
GCTTTATTAC CTCCTGAGAT CTATGCAGGA AGCCTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACCTGA CTTCTTCTGA TACCCAGATG GCAGCATCCA CTTTCTTACT TCCACCCCTC 
TGTCACCAAG ATGATCTTTT ATTACTAAGT CCTTTGCTAC AAGACACTGA CCACAGATTT 
ATTGAAGAAC AGCTCCTCCT TGGTAATGGT GTTGCTTGTG GAAGTCTAGA GTATGACCCA 
TTTTCAGTTT ATGAACATGG CATAGAAGGG GATTCAGTAC CTAAGCCCCT ACCTCCAATG 
TTGTCACTTA TCAGCTCTGC AGCCAGGCTT TTTGGAATTG TATGTGCCCA TGTAGCAGAA 
TCTCAAAGGC TCTTGGTATT GGAACAACTT TTGGACAAAA TAAAACACAC AAAAGCATCT 
CGTCAACAAG TAGTACAATT ACATGTTATG GCAGCATTTT CTAATTTTTT GAAGTATGTG 
GCCAACTCCA AAGGAAATTT AGGCTCAGAA GAAATTAGAA GACCTGCACT AACATTATTG 
ATGGGAGCCT TGGAAAGTAC CAACCCCTTA ATGCGTTGTA CTGCTGCAGA AGGTTTGGCT 
AGATTAGCCC AAGTGATTGG TGATGGCTCC TTCACTGCTG GATTAGCACA GGTTAGCTTT 
GAAAAACTGA AATCAGCTAG GGATGTGGTA ACCAGAACAG GACATTCTTT GGCTCTGGGA 
TCCTTACATA GATATTTAGG AGGAGTGGGC TCTTCTCAGC ACTTAAATGC TTGCATCGGA 
ATACTTTACA CTCTGTCACA GGATGGAACT TCTCCTGATG TACAGACATG GGCCCTACAT 
TCTCTCTCAT TGATAATTGA TTCTGCTGGT CCACTGTACC ATTTACATGT GGAATCTACC 
CTCTCTCTTA TACTAATGTT GTTGTTGACT GTTCCACCTA CTCATGCTGA AGTTCACCAA 
AGTCTTGGAC GATGTTTGAA TGTACTCATC ACCACATTGG GTCCAGAACT ACAAGGTAGC 
AGTACTGCAG TTTCTACTTT ACGGACATCC TGTCTTTTGG GGTGTGCAAT AATGCAGGAT 
AACCCAGATT GCCTTGTTCA AGCTCAAGCC ATCTCTTGTC TTCAACAACT TCATATGTTT 
GCCCCACGCT ATGTCAATTT ATCTAGTCTA GTCAGCTGTC TTTGTGTGAA TCTCTGTAGC 
TCCTACTTGT TTCTGAGAAG AGCAGTACTA GCTTGTTTAC GTCAAATTGT GCAAAGGGAA 
GCAGCTGAGG TTTCAGAACA TGCTGTTGTT CTTGCTAAGG ACAGCAGAGA AGACTTAATT 
CCAGATGCTA ATATCCGAGA AGTGGGTCTT GAGGGGGCAT TGTTGAACTT ACTAGACAAA 
GAATCTGATC AGAGATTGTG CCAGGATATC AAAGAGACCC TGAGTCATAT GATCACATCA 
ATGGCAGTGG GAAAGCTTTC TTTTTGGCTA AAACTTTGTA AAGATGTCCT TGCTTCATCA 
GCTGATTTTA CAACTGTTGC TTCTGTGGAT ACAACTCAGG ATGATGAAAT AGATAAAGAA 
GATGATGCTT CAGTCCTAAC CTACAGAAAT GATGAGAAAT GCCGGCCAAT TACAAATCCA 
CGATGGGCTA CTAGAGTTTT TGCTGCTGAA TGTGTGTGTA AAATAATCAG CCAGTGTGAG 
AATTCAGACA GTGCTCACTT TGACATTGCT TTGGCTCAAG AAATGAAAGA AAGGGATTCA 
CGAAATGATT TTTTGGTATT ACATCTTGCT GACTTGATCC GCATGGCTTT TATGGCTGCA 
ACAGACCACA GTGACCAGCT CCGTCTTTCT GGTCTTCAGA CACTGTTAGT AGTTGTTCGG 
CAATTTGCAA CTATTCCTGA ACCAGAATTC CCAGGTCATG TGATTCTGGA ACAGTATCAA 
GCCAATGTGG GAGCAGCACT TAGACCAGCT TTTACTTCAG ATACACCACC AGATGTAACT 
GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG ATAGCAAGTG GAGTTGTAAG TGATCTTAAT 
GATCTCCGAA GAGTTCATCA ATTGCTTGTA TCATCCTTGA CGAAAATACA GGCTGGAAAA 
GAAGCCTTGA GTCAATTATA TAACGAAAGT GCTTCTACTA TGGAAATCCT AGCTGTTCTC 
AAAGCCTGGG CAGAGGTTTA CATAATTGCT GTAGAAAGAC AGAAAAAACA AACTGAGAAA 
CAGAAGCAGA CTATGAAAAT TGTTGGCTCT ATAGAGGAAA ATGCCAGAGA TGTGTCATCT 
TCCATAGATG GACTTCTTGA CTTAGTCCAA ACAGATCTTG GAACATTAAG CAAACTCTGG 
CTTGCTGCTC TTCAAGATTT TGCTCTTCTA ACTTTGCCTT CAGAATTTGC TTCCCAACTT 
CCTCTTGAAG GTGGAGCTTT CTACACTGCA GAAACTAGTG AAAATGCAAA GCCACACTAT 
CATAATTCGT GGGCTCTTAT TCTTCATGCT ACAGCATTGT GGCTCACTAG CACAGGCTTC 
ATTGTAGCTG ACCCAGATGA AGGAGCAACT AATCTCTCAA GACCTGTAAC ACCGACTACA 
ATGTGTCAGG GTTCTTCTGG GGCATCAGTG AAATCCCCTG AGGATGTTTA TACAGACAGA 
TTTCACCTTA TCTTGGGAAT CAGTGTGGAA TTTCTCTGTT CCCTGCGTTC AGATGCAACC 
ATGGAAAGCA TCACTGCTTG TTTACATGCA CTGCAGGCTT TACTGGATGT TCCTTGGCCC 
AGATCTAAGA TTGGCAGTGA CCAGGACTTG GGAGTTGAGT TGTTGAATGT ACTCCATCGA 
GTAATACTGA CTAGAGAGTC ACCTTCCATT CAGTTAGCTT CACTTGAAGT TGTAAGACAG 
GTTATCTACG CAGCCCAAGA ACATGTAAAG GAAAAGAGGA GAAGTGCAGA AGTTGATGAT 
GGAGCTGCTG AAAAGGAAAC CCTGCCAGAA TTTGGAGAAG GAAAGGATAC GGGAGGACTG 
GTACCTGGAA GATCTCTGGT GTTTGCAACA TTGGAGCTAT GTGTATGCAT TTTGGTCCGG 
CAACTTCCAG AGTTAAATCC TAAACTGACC GGTAGTCCAG GAATAAAAAC TACAAAAGCA 
CAGATTCTTT CAGATGATGG AAATAAGTTG GTTTCAGCAG CATTAACTAT CCTCTCAGAA 
CTTCCTGGAG TATGCTCTCC CGAAGGAAGC ATTTCAGTTC TCCCTACTAT TCTGTACCTT 
ATAATTGGGG TCCTCCGAGA AACTGCTGTG AGGTTACCTG GAGGACAGTT ATCTTATACT 
GTTGGAGCTG CTCTACAAGC CTTGAAAGGA ATATTGGCAT CTCCCTTGGC TCGAGCAGAG 
AAGAGTCACA CTGCTTGGAC TGACCTTCTT CGAAGTGCTT TGACAACAAT CCTAGACTGC 
TGGGATCCAG ATGGAACACA GCAAGAGTTT GATGAAATCA GCCTGCTTAC TGCCATAACA 
GTATTTATCT TGTCTTCAAG TCCAGAAGTG ACCACCATCC AGTGCCTGCA AGAACGTTGT 
ATACAGAAGT TTAAAGCTAC TCTTGAGTTA AAAGACCCAG TAGTACAAAT AAAGTGCTAT 
CAGCTACTGC TTTCCATTTT CCAGTATCCA AACCCAGCAG TATCCTACCC TTATATTCAT 
TCATTAGCAT CATCTATTGT GAGAAAACTG CAAGAGACAG ATAAAGTAAA ACCAGAAGAT 
GCTGCTGAGC TTCAGGTGAT TCAAGAAGGT ATTAAGGTCT TAGAAGCCCT AGTAGGGATT 
GCGGAAGAAC AGCACCGTGC TCACTTGGTG GCCGACATTC TCCCTATTCT TATTTCCTTT 
CTTTTGGATG AAAACACTCT GGGCTCAGCA CCCACCACAA TGAGAAATTT ACATGACTTT 
GCTTTGCAGA ATCTGATGCA GATTGGTCCC CAGTATTCAT CAACTTTTAA AAATATTATG 
GCTTCTTCTC CCACAATGAA AGCCCGTCTT GAGGCAGCTA TAAAAGGCAA TCAGGAAAAT 
ATCAAAATCA AGACAACCCC AAAACATATC ACAAAACCTG GAAAAAATTC AAGTATTCAG 
TTGAAGACCA ATTTTTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012402435.1
CDS	375..6515
Translation	MELAHSLLLNEEAYNQLGEVQKAEFVFEWLKFLEKLLLATNKADIKEKQKSIIEQLISLLNSSPGPPTRKLLAENLAVLYSIGDTFSVYQTIDKCNELIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRLLGNTFIDTVGNILKAMKSAESQGRYEIMLSLQNILSGLGAAAAPCHRDIYKAARSCLTDRSMAVRCAAAKCLLELQNEASFMWSTDIDSVATLCFKSFEGSNYDVRIAVSKLLGTVLARAVMSKPGTATSRQNVRKVSLEEAMELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFISTLGGRWLERNFSILLSHILSLVSQFHPKAIQTQMDAICCRRCVSFILRTTIGSLLGEKAQIAAARDICQVIWKLKKTVDAVMSEVNLETRPGSTDVAASQHVLVCALQELGNLIYGLGTTAAPLLQDSSAGILDNVISVIVHPSISVRLAAAWCLHCIAVALPSYLTPLLDSCLERLTVLKSSPEAVTGFSFAVAALLGAVKYCPLGIPHGKGKIIVMLAEDLLCSAAQNSRLSVQRTQAGWLLIAALMTLGSAFIHHHLPRVLRLWKCVFPLSPKDLETERSRGDSFTWQVTLEGRAGAFCAIKSFVSHCNDLLTDEVIQRLLPPLPCAVNLLTQLSSIQKTYGSPLKTPSVVYKQRLYELLALLPPEIYAGSLCAILKELAADLTSSDTQMAASTFLLPPLCHQDDLLLLSPLLQDTDHRFIEEQLLLGNGVACGSLEYDPFSVYEHGIEGDSVPKPLPPMLSLISSAARLFGIVCAHVAESQRLLVLEQLLDKIKHTKASRQQVVQLHVMAAFSNFLKYVANSKGNLGSEEIRRPALTLLMGALESTNPLMRCTAAEGLARLAQVIGDGSFTAGLAQVSFEKLKSARDVVTRTGHSLALGSLHRYLGGVGSSQHLNACIGILYTLSQDGTSPDVQTWALHSLSLIIDSAGPLYHLHVESTLSLILMLLLTVPPTHAEVHQSLGRCLNVLITTLGPELQGSSTAVSTLRTSCLLGCAIMQDNPDCLVQAQAISCLQQLHMFAPRYVNLSSLVSCLCVNLCSSYLFLRRAVLACLRQIVQREAAEVSEHAVVLAKDSREDLIPDANIREVGLEGALLNLLDKESDQRLCQDIKETLSHMITSMAVGKLSFWLKLCKDVLASSADFTTVASVDTTQDDEIDKEDDASVLTYRNDEKCRPITNPRWATRVFAAECVCKIISQCENSDSAHFDIALAQEMKERDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLQTLLVVVRQFATIPEPEFPGHVILEQYQANVGAALRPAFTSDTPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSQLYNESASTMEILAVLKAWAEVYIIAVERQKKQTEKQKQTMKIVGSIEENARDVSSSIDGLLDLVQTDLGTLSKLWLAALQDFALLTLPSEFASQLPLEGGAFYTAETSENAKPHYHNSWALILHATALWLTSTGFIVADPDEGATNLSRPVTPTTMCQGSSGASVKSPEDVYTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGVELLNVLHRVILTRESPSIQLASLEVVRQVIYAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGRSLVFATLELCVCILVRQLPELNPKLTGSPGIKTTKAQILSDDGNKLVSAALTILSELPGVCSPEGSISVLPTILYLIIGVLRETAVRLPGGQLSYTVGAALQALKGILASPLARAEKSHTAWTDLLRSALTTILDCWDPDGTQQEFDEISLLTAITVFILSSSPEVTTIQCLQERCIQKFKATLELKDPVVQIKCYQLLLSIFQYPNPAVSYPYIHSLASSIVRKLQETDKVKPEDAAELQVIQEGIKVLEALVGIAEEQHRAHLVADILPILISFLLDENTLGSAPTTMRNLHDFALQNLMQIGPQYSSTFKNIMASSPTMKARLEAAIKGNQENIKIKTTPKHITKPGKNSSIQLKTNFF

Target ORF information:

RefSeq Version	XM_012546981.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012546981.2

ORF Insert Sequence:

ATGGAACTGG CTCACAGCTT ATTGCTGAAT GAAGAAGCAT ATAATCAACT TGGAGAAGTT 
CAGAAGGCAG AATTTGTTTT TGAATGGTTG AAATTTTTAG AGAAACTTTT GCTAGCAACC 
AACAAGGCTG ACATAAAGGA GAAACAGAAG AGTATTATTG AGCAGCTCAT ATCTCTATTA 
AATAGCTCTC CAGGGCCACC TACCCGAAAG CTTCTTGCTG AGAACCTAGC TGTTCTTTAT 
AGTATTGGAG ATACATTTTC TGTTTATCAA ACAATTGACA AATGTAATGA ACTCATTCGT 
AGCAAAGATG ACTCTCCAAG TTATCTTCCC ACAAAACTTG CTGCTGTGGT ATGTCTGGGT 
TCTTTGTATA AGAAGTTGGG GAGACTATTG GGTAACACCT TTATAGATAC AGTGGGGAAT 
ATACTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGTCGAT ATGAGATTAT GCTGAGTTTG 
CAAAATATAC TGAGTGGACT TGGAGCTGCT GCTGCCCCTT GTCACAGAGA CATTTATAAA 
GCTGCAAGGT CATGTTTGAC AGACAGGTCC ATGGCAGTTC GCTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAGAATGA AGCTAGCTTT ATGTGGAGTA CAGACATAGA CAGTGTTGCA 
ACTTTGTGTT TTAAGTCATT TGAAGGTTCC AACTATGATG TGCGAATAGC AGTATCAAAG 
CTCTTAGGTA CAGTGTTGGC TAGAGCTGTA ATGTCCAAAC CTGGAACAGC AACTTCCCGG 
CAAAATGTTC GCAAAGTTTC TCTGGAGGAA GCCATGGAGT TACTAGGAAC AGGGTTTCTA 
CGTGGGAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGT TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG TGTTACTCAG GCATATGTGG TGTTTATTTC AACACTAGGA 
GGACGTTGGT TAGAAAGAAA TTTTTCCATC TTACTCTCTC ATATCCTTAG CCTAGTATCA 
CAGTTTCACC CTAAAGCAAT CCAAACTCAG ATGGATGCTA TCTGTTGTCG TCGCTGTGTT 
TCATTTATCC TTCGAACTAC TATAGGAAGC CTTCTCGGGG AAAAAGCTCA AATTGCTGCT 
GCCAGGGATA TTTGTCAGGT CATCTGGAAA CTAAAGAAAA CTGTTGATGC TGTAATGAGT 
GAAGTTAATT TGGAAACTCG ACCTGGCTCC ACAGATGTTG CTGCCAGCCA GCATGTGCTA 
GTATGTGCTT TACAAGAACT TGGAAATCTA ATATATGGTC TTGGCACAAC AGCTGCACCT 
TTATTACAGG ATTCAAGTGC AGGGATTCTT GACAATGTCA TTTCAGTTAT TGTTCATCCC 
AGCATTTCAG TTCGATTAGC AGCAGCTTGG TGTTTACACT GCATTGCTGT AGCATTGCCT 
TCCTACCTAA CTCCACTTTT GGACAGTTGC CTTGAACGAC TTACTGTGCT CAAATCCTCA 
CCTGAAGCTG TGACTGGCTT TAGTTTTGCT GTGGCAGCTC TACTAGGAGC AGTGAAATAT 
TGCCCTTTAG GAATTCCACA TGGAAAAGGA AAGATAATTG TGATGTTAGC AGAGGATTTG 
TTATGTTCTG CTGCTCAGAA TAGTCGCCTT TCAGTTCAAC GAACACAAGC TGGATGGTTG 
CTGATAGCTG CCTTAATGAC ATTAGGCTCT GCATTTATTC ATCACCACCT TCCCCGGGTG 
CTAAGATTAT GGAAATGTGT TTTTCCACTA TCTCCTAAGG ATCTAGAAAC AGAAAGAAGT 
CGAGGAGACT CATTTACATG GCAGGTGACC TTGGAAGGTC GTGCAGGGGC CTTCTGTGCT 
ATCAAGAGCT TTGTTTCCCA CTGTAATGAT CTTCTTACTG ATGAAGTCAT TCAGAGACTT 
CTTCCTCCCC TTCCGTGTGC TGTTAATTTA TTAACTCAAC TTTCTTCAAT ACAGAAAACA 
TATGGAAGTC CTTTAAAAAC TCCTTCAGTG GTTTACAAAC AAAGACTTTA TGAACTATTG 
GCTTTATTAC CTCCTGAGAT CTATGCAGGA AGCCTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACCTGA CTTCTTCTGA TACCCAGATG GCAGCATCCA CTTTCTTACT TCCACCCCTC 
TGTCACCAAG ATGATCTTTT ATTACTAAGT CCTTTGCTAC AAGACACTGA CCACAGATTT 
ATTGAAGAAC AGCTCCTCCT TGGTAATGGT GTTGCTTGTG GAAGTCTAGA GTATGACCCA 
TTTTCAGTTT ATGAACATGG CATAGAAGGG GATTCAGTAC CTAAGCCCCT ACCTCCAATG 
TTGTCACTTA TCAGCTCTGC AGCCAGGCTT TTTGGAATTG TATGTGCCCA TGTAGCAGAA 
TCTCAAAGGC TCTTGGTATT GGAACAACTT TTGGACAAAA TAAAACACAC AAAAGCATCT 
CGTCAACAAG TAGTACAATT ACATGTTATG GCAGCATTTT CTAATTTTTT GAAGTATGTG 
GCCAACTCCA AAGGAAATTT AGGCTCAGAA GAAATTAGAA GACCTGCACT AACATTATTG 
ATGGGAGCCT TGGAAAGTAC CAACCCCTTA ATGCGTTGTA CTGCTGCAGA AGGTTTGGCT 
AGATTAGCCC AAGTGATTGG TGATGGCTCC TTCACTGCTG GATTAGCACA GGTTAGCTTT 
GAAAAACTGA AATCAGCTAG GGATGTGGTA ACCAGAACAG GACATTCTTT GGCTCTGGGA 
TCCTTACATA GATATTTAGG AGGAGTGGGC TCTTCTCAGC ACTTAAATGC TTGCATCGGA 
ATACTTTACA CTCTGTCACA GGATGGAACT TCTCCTGATG TACAGACATG GGCCCTACAT 
TCTCTCTCAT TGATAATTGA TTCTGCTGGT CCACTGTACC ATTTACATGT GGAATCTACC 
CTCTCTCTTA TACTAATGTT GTTGTTGACT GTTCCACCTA CTCATGCTGA AGTTCACCAA 
AGTCTTGGAC GATGTTTGAA TGTACTCATC ACCACATTGG GTCCAGAACT ACAAGGTAGC 
AGTACTGCAG TTTCTACTTT ACGGACATCC TGTCTTTTGG GGTGTGCAAT AATGCAGGAT 
AACCCAGATT GCCTTGTTCA AGCTCAAGCC ATCTCTTGTC TTCAACAACT TCATATGTTT 
GCCCCACGCT ATGTCAATTT ATCTAGTCTA GTCAGCTGTC TTTGTGTGAA TCTCTGTAGC 
TCCTACTTGT TTCTGAGAAG AGCAGTACTA GCTTGTTTAC GTCAAATTGT GCAAAGGGAA 
GCAGCTGAGG TTTCAGAACA TGCTGTTGTT CTTGCTAAGG ACAGCAGAGA AGACTTAATT 
CCAGATGCTA ATATCCGAGA AGTGGGTCTT GAGGGGGCAT TGTTGAACTT ACTAGACAAA 
GAATCTGATC AGAGATTGTG CCAGGATATC AAAGAGACCC TGAGTCATAT GATCACATCA 
ATGGCAGTGG GAAAGCTTTC TTTTTGGCTA AAACTTTGTA AAGATGTCCT TGCTTCATCA 
GCTGATTTTA CAACTGTTGC TTCTGTGGAT ACAACTCAGG ATGATGAAAT AGATAAAGAA 
GATGATGCTT CAGTCCTAAC CTACAGAAAT GATGAGAAAT GCCGGCCAAT TACAAATCCA 
CGATGGGCTA CTAGAGTTTT TGCTGCTGAA TGTGTGTGTA AAATAATCAG CCAGTGTGAG 
AATTCAGACA GTGCTCACTT TGACATTGCT TTGGCTCAAG AAATGAAAGA AAGGGATTCA 
CGAAATGATT TTTTGGTATT ACATCTTGCT GACTTGATCC GCATGGCTTT TATGGCTGCA 
ACAGACCACA GTGACCAGCT CCGTCTTTCT GGTCTTCAGA CACTGTTAGT AGTTGTTCGG 
CAATTTGCAA CTATTCCTGA ACCAGAATTC CCAGGTCATG TGATTCTGGA ACAGTATCAA 
GCCAATGTGG GAGCAGCACT TAGACCAGCT TTTACTTCAG ATACACCACC AGATGTAACT 
GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG ATAGCAAGTG GAGTTGTAAG TGATCTTAAT 
GATCTCCGAA GAGTTCATCA ATTGCTTGTA TCATCCTTGA CGAAAATACA GGCTGGAAAA 
GAAGCCTTGA GTCAATTATA TAACGAAAGT GCTTCTACTA TGGAAATCCT AGCTGTTCTC 
AAAGCCTGGG CAGAGGTTTA CATAATTGCT GTAGAAAGAC AGAAAAAACA AACTGAGAAA 
CAGAAGCAGA CTATGAAAAT TGTTGGCTCT ATAGAGGAAA ATGCCAGAGA TGTGTCATCT 
TCCATAGATG GACTTCTTGA CTTAGTCCAA ACAGATCTTG GAACATTAAG CAAACTCTGG 
CTTGCTGCTC TTCAAGATTT TGCTCTTCTA ACTTTGCCTT CAGAATTTGC TTCCCAACTT 
CCTCTTGAAG GTGGAGCTTT CTACACTGCA GAAACTAGTG AAAATGCAAA GCCACACTAT 
CATAATTCGT GGGCTCTTAT TCTTCATGCT ACAGCATTGT GGCTCACTAG CACAGGCTTC 
ATTGTAGCTG ACCCAGATGA AGGAGCAACT AATCTCTCAA GACCTGTAAC ACCGACTACA 
ATGTGTCAGG GTTCTTCTGG GGCATCAGTG AAATCCCCTG AGGATGTTTA TACAGACAGA 
TTTCACCTTA TCTTGGGAAT CAGTGTGGAA TTTCTCTGTT CCCTGCGTTC AGATGCAACC 
ATGGAAAGCA TCACTGCTTG TTTACATGCA CTGCAGGCTT TACTGGATGT TCCTTGGCCC 
AGATCTAAGA TTGGCAGTGA CCAGGACTTG GGAGTTGAGT TGTTGAATGT ACTCCATCGA 
GTAATACTGA CTAGAGAGTC ACCTTCCATT CAGTTAGCTT CACTTGAAGT TGTAAGACAG 
GTTATCTACG CAGCCCAAGA ACATGTAAAG GAAAAGAGGA GAAGTGCAGA AGTTGATGAT 
GGAGCTGCTG AAAAGGAAAC CCTGCCAGAA TTTGGAGAAG GAAAGGATAC GGGAGGACTG 
GTACCTGGAA GATCTCTGGT GTTTGCAACA TTGGAGCTAT GTGTATGCAT TTTGGTCCGG 
CAACTTCCAG AGTTAAATCC TAAACTGACC GGTAGTCCAG GAATAAAAAC TACAAAAGCA 
CAGATTCTTT CAGATGATGG AAATAAGTTG GTTTCAGCAG CATTAACTAT CCTCTCAGAA 
CTTCCTGGAG TATGCTCTCC CGAAGGAAGC ATTTCAGTTC TCCCTACTAT TCTGTACCTT 
ATAATTGGGG TCCTCCGAGA AACTGCTGTG AGGTTACCTG GAGGACAGTT ATCTTATACT 
GTTGGAGCTG CTCTACAAGC CTTGAAAGGA ATATTGGCAT CTCCCTTGGC TCGAGCAGAG 
AAGAGTCACA CTGCTTGGAC TGACCTTCTT CGAAGTGCTT TGACAACAAT CCTAGACTGC 
TGGGATCCAG ATGGAACACA GCAAGAGTTT GATGAAATCA GCCTGCTTAC TGCCATAACA 
GTATTTATCT TGTCTTCAAG TCCAGAAGTG ACCACCATCC AGTGCCTGCA AGAACGTTGT 
ATACAGAAGT TTAAAGCTAC TCTTGAGTTA AAAGACCCAG TAGTACAAAT AAAGTGCTAT 
CAGCTACTGC TTTCCATTTT CCAGTATCCA AACCCAGCAG TATCCTACCC TTATATTCAT 
TCATTAGCAT CATCTATTGT GAGAAAACTG CAAGAGACAG ATAAAGTAAA ACCAGAAGAT 
GCTGCTGAGC TTCAGGTGAT TCAAGAAGGT ATTAAGGTCT TAGAAGCCCT AGTAGGGATT 
GCGGAAGAAC AGCACCGTGC TCACTTGGTG GCCGACATTC TCCCTATTCT TATTTCCTTT 
CTTTTGGATG AAAACACTCT GGGCTCAGCA CCCACCACAA TGAGAAATTT ACATGACTTT 
GCTTTGCAGA ATCTGATGCA GATTGGTCCC CAGTATTCAT CAACTTTTAA AAATATTATG 
GCTTCTTCTC CCACAATGAA AGCCCGTCTT GAGGCAGCTA TAAAAGGCAA TCAGGAAAAT 
ATCAAAATCA AGACAACCCC AAAACATATC ACAAAACCTG GAAAAAATTC AAGTATTCAG 
TTGAAGACCA ATTTTTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_012546981.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012402435.1 Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), mRNA.

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CloneID	OSa117582
Clone ID Related Accession (Same CDS sequence)	XM_023501135.1 , XM_023501135.2 , XM_012546981.2 , XM_012546981.1
Accession Version	XM_012546981.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 5A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816524.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG CTCACAGCTT ATTGCTGAAT GAAGAAGCAT ATAATCAACT TGGAGAAGTT 
CAGAAGGCAG AATTTGTTTT TGAATGGTTG AAATTTTTAG AGAAACTTTT GCTAGCAACC 
AACAAGGCTG ACATAAAGGA GAAACAGAAG AGTATTATTG AGCAGCTCAT ATCTCTATTA 
AATAGCTCTC CAGGGCCACC TACCCGAAAG CTTCTTGCTG AGAACCTAGC TGTTCTTTAT 
AGTATTGGAG ATACATTTTC TGTTTATCAA ACAATTGACA AATGTAATGA ACTCATTCGT 
AGCAAAGATG ACTCTCCAAG TTATCTTCCC ACAAAACTTG CTGCTGTGGT ATGTCTGGGT 
TCTTTGTATA AGAAGTTGGG GAGACTATTG GGTAACACCT TTATAGATAC AGTGGGGAAT 
ATACTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGTCGAT ATGAGATTAT GCTGAGTTTG 
CAAAATATAC TGAGTGGACT TGGAGCTGCT GCTGCCCCTT GTCACAGAGA CATTTATAAA 
GCTGCAAGGT CATGTTTGAC AGACAGGTCC ATGGCAGTTC GCTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAGAATGA AGCTAGCTTT ATGTGGAGTA CAGACATAGA CAGTGTTGCA 
ACTTTGTGTT TTAAGTCATT TGAAGGTTCC AACTATGATG TGCGAATAGC AGTATCAAAG 
CTCTTAGGTA CAGTGTTGGC TAGAGCTGTA ATGTCCAAAC CTGGAACAGC AACTTCCCGG 
CAAAATGTTC GCAAAGTTTC TCTGGAGGAA GCCATGGAGT TACTAGGAAC AGGGTTTCTA 
CGTGGGAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGT TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG TGTTACTCAG GCATATGTGG TGTTTATTTC AACACTAGGA 
GGACGTTGGT TAGAAAGAAA TTTTTCCATC TTACTCTCTC ATATCCTTAG CCTAGTATCA 
CAGTTTCACC CTAAAGCAAT CCAAACTCAG ATGGATGCTA TCTGTTGTCG TCGCTGTGTT 
TCATTTATCC TTCGAACTAC TATAGGAAGC CTTCTCGGGG AAAAAGCTCA AATTGCTGCT 
GCCAGGGATA TTTGTCAGGT CATCTGGAAA CTAAAGAAAA CTGTTGATGC TGTAATGAGT 
GAAGTTAATT TGGAAACTCG ACCTGGCTCC ACAGATGTTG CTGCCAGCCA GCATGTGCTA 
GTATGTGCTT TACAAGAACT TGGAAATCTA ATATATGGTC TTGGCACAAC AGCTGCACCT 
TTATTACAGG ATTCAAGTGC AGGGATTCTT GACAATGTCA TTTCAGTTAT TGTTCATCCC 
AGCATTTCAG TTCGATTAGC AGCAGCTTGG TGTTTACACT GCATTGCTGT AGCATTGCCT 
TCCTACCTAA CTCCACTTTT GGACAGTTGC CTTGAACGAC TTACTGTGCT CAAATCCTCA 
CCTGAAGCTG TGACTGGCTT TAGTTTTGCT GTGGCAGCTC TACTAGGAGC AGTGAAATAT 
TGCCCTTTAG GAATTCCACA TGGAAAAGGA AAGATAATTG TGATGTTAGC AGAGGATTTG 
TTATGTTCTG CTGCTCAGAA TAGTCGCCTT TCAGTTCAAC GAACACAAGC TGGATGGTTG 
CTGATAGCTG CCTTAATGAC ATTAGGCTCT GCATTTATTC ATCACCACCT TCCCCGGGTG 
CTAAGATTAT GGAAATGTGT TTTTCCACTA TCTCCTAAGG ATCTAGAAAC AGAAAGAAGT 
CGAGGAGACT CATTTACATG GCAGGTGACC TTGGAAGGTC GTGCAGGGGC CTTCTGTGCT 
ATCAAGAGCT TTGTTTCCCA CTGTAATGAT CTTCTTACTG ATGAAGTCAT TCAGAGACTT 
CTTCCTCCCC TTCCGTGTGC TGTTAATTTA TTAACTCAAC TTTCTTCAAT ACAGAAAACA 
TATGGAAGTC CTTTAAAAAC TCCTTCAGTG GTTTACAAAC AAAGACTTTA TGAACTATTG 
GCTTTATTAC CTCCTGAGAT CTATGCAGGA AGCCTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACCTGA CTTCTTCTGA TACCCAGATG GCAGCATCCA CTTTCTTACT TCCACCCCTC 
TGTCACCAAG ATGATCTTTT ATTACTAAGT CCTTTGCTAC AAGACACTGA CCACAGATTT 
ATTGAAGAAC AGCTCCTCCT TGGTAATGGT GTTGCTTGTG GAAGTCTAGA GTATGACCCA 
TTTTCAGTTT ATGAACATGG CATAGAAGGG GATTCAGTAC CTAAGCCCCT ACCTCCAATG 
TTGTCACTTA TCAGCTCTGC AGCCAGGCTT TTTGGAATTG TATGTGCCCA TGTAGCAGAA 
TCTCAAAGGC TCTTGGTATT GGAACAACTT TTGGACAAAA TAAAACACAC AAAAGCATCT 
CGTCAACAAG TAGTACAATT ACATGTTATG GCAGCATTTT CTAATTTTTT GAAGTATGTG 
GCCAACTCCA AAGGAAATTT AGGCTCAGAA GAAATTAGAA GACCTGCACT AACATTATTG 
ATGGGAGCCT TGGAAAGTAC CAACCCCTTA ATGCGTTGTA CTGCTGCAGA AGGTTTGGCT 
AGATTAGCCC AAGTGATTGG TGATGGCTCC TTCACTGCTG GATTAGCACA GGTTAGCTTT 
GAAAAACTGA AATCAGCTAG GGATGTGGTA ACCAGAACAG GACATTCTTT GGCTCTGGGA 
TCCTTACATA GATATTTAGG AGGAGTGGGC TCTTCTCAGC ACTTAAATGC TTGCATCGGA 
ATACTTTACA CTCTGTCACA GGATGGAACT TCTCCTGATG TACAGACATG GGCCCTACAT 
TCTCTCTCAT TGATAATTGA TTCTGCTGGT CCACTGTACC ATTTACATGT GGAATCTACC 
CTCTCTCTTA TACTAATGTT GTTGTTGACT GTTCCACCTA CTCATGCTGA AGTTCACCAA 
AGTCTTGGAC GATGTTTGAA TGTACTCATC ACCACATTGG GTCCAGAACT ACAAGGTAGC 
AGTACTGCAG TTTCTACTTT ACGGACATCC TGTCTTTTGG GGTGTGCAAT AATGCAGGAT 
AACCCAGATT GCCTTGTTCA AGCTCAAGCC ATCTCTTGTC TTCAACAACT TCATATGTTT 
GCCCCACGCT ATGTCAATTT ATCTAGTCTA GTCAGCTGTC TTTGTGTGAA TCTCTGTAGC 
TCCTACTTGT TTCTGAGAAG AGCAGTACTA GCTTGTTTAC GTCAAATTGT GCAAAGGGAA 
GCAGCTGAGG TTTCAGAACA TGCTGTTGTT CTTGCTAAGG ACAGCAGAGA AGACTTAATT 
CCAGATGCTA ATATCCGAGA AGTGGGTCTT GAGGGGGCAT TGTTGAACTT ACTAGACAAA 
GAATCTGATC AGAGATTGTG CCAGGATATC AAAGAGACCC TGAGTCATAT GATCACATCA 
ATGGCAGTGG GAAAGCTTTC TTTTTGGCTA AAACTTTGTA AAGATGTCCT TGCTTCATCA 
GCTGATTTTA CAACTGTTGC TTCTGTGGAT ACAACTCAGG ATGATGAAAT AGATAAAGAA 
GATGATGCTT CAGTCCTAAC CTACAGAAAT GATGAGAAAT GCCGGCCAAT TACAAATCCA 
CGATGGGCTA CTAGAGTTTT TGCTGCTGAA TGTGTGTGTA AAATAATCAG CCAGTGTGAG 
AATTCAGACA GTGCTCACTT TGACATTGCT TTGGCTCAAG AAATGAAAGA AAGGGATTCA 
CGAAATGATT TTTTGGTATT ACATCTTGCT GACTTGATCC GCATGGCTTT TATGGCTGCA 
ACAGACCACA GTGACCAGCT CCGTCTTTCT GGTCTTCAGA CACTGTTAGT AGTTGTTCGG 
CAATTTGCAA CTATTCCTGA ACCAGAATTC CCAGGTCATG TGATTCTGGA ACAGTATCAA 
GCCAATGTGG GAGCAGCACT TAGACCAGCT TTTACTTCAG ATACACCACC AGATGTAACT 
GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG ATAGCAAGTG GAGTTGTAAG TGATCTTAAT 
GATCTCCGAA GAGTTCATCA ATTGCTTGTA TCATCCTTGA CGAAAATACA GGCTGGAAAA 
GAAGCCTTGA GTCAATTATA TAACGAAAGT GCTTCTACTA TGGAAATCCT AGCTGTTCTC 
AAAGCCTGGG CAGAGGTTTA CATAATTGCT GTAGAAAGAC AGAAAAAACA AACTGAGAAA 
CAGAAGCAGA CTATGAAAAT TGTTGGCTCT ATAGAGGAAA ATGCCAGAGA TGTGTCATCT 
TCCATAGATG GACTTCTTGA CTTAGTCCAA ACAGATCTTG GAACATTAAG CAAACTCTGG 
CTTGCTGCTC TTCAAGATTT TGCTCTTCTA ACTTTGCCTT CAGAATTTGC TTCCCAACTT 
CCTCTTGAAG GTGGAGCTTT CTACACTGCA GAAACTAGTG AAAATGCAAA GCCACACTAT 
CATAATTCGT GGGCTCTTAT TCTTCATGCT ACAGCATTGT GGCTCACTAG CACAGGCTTC 
ATTGTAGCTG ACCCAGATGA AGGAGCAACT AATCTCTCAA GACCTGTAAC ACCGACTACA 
ATGTGTCAGG GTTCTTCTGG GGCATCAGTG AAATCCCCTG AGGATGTTTA TACAGACAGA 
TTTCACCTTA TCTTGGGAAT CAGTGTGGAA TTTCTCTGTT CCCTGCGTTC AGATGCAACC 
ATGGAAAGCA TCACTGCTTG TTTACATGCA CTGCAGGCTT TACTGGATGT TCCTTGGCCC 
AGATCTAAGA TTGGCAGTGA CCAGGACTTG GGAGTTGAGT TGTTGAATGT ACTCCATCGA 
GTAATACTGA CTAGAGAGTC ACCTTCCATT CAGTTAGCTT CACTTGAAGT TGTAAGACAG 
GTTATCTACG CAGCCCAAGA ACATGTAAAG GAAAAGAGGA GAAGTGCAGA AGTTGATGAT 
GGAGCTGCTG AAAAGGAAAC CCTGCCAGAA TTTGGAGAAG GAAAGGATAC GGGAGGACTG 
GTACCTGGAA GATCTCTGGT GTTTGCAACA TTGGAGCTAT GTGTATGCAT TTTGGTCCGG 
CAACTTCCAG AGTTAAATCC TAAACTGACC GGTAGTCCAG GAATAAAAAC TACAAAAGCA 
CAGATTCTTT CAGATGATGG AAATAAGTTG GTTTCAGCAG CATTAACTAT CCTCTCAGAA 
CTTCCTGGAG TATGCTCTCC CGAAGGAAGC ATTTCAGTTC TCCCTACTAT TCTGTACCTT 
ATAATTGGGG TCCTCCGAGA AACTGCTGTG AGGTTACCTG GAGGACAGTT ATCTTATACT 
GTTGGAGCTG CTCTACAAGC CTTGAAAGGA ATATTGGCAT CTCCCTTGGC TCGAGCAGAG 
AAGAGTCACA CTGCTTGGAC TGACCTTCTT CGAAGTGCTT TGACAACAAT CCTAGACTGC 
TGGGATCCAG ATGGAACACA GCAAGAGTTT GATGAAATCA GCCTGCTTAC TGCCATAACA 
GTATTTATCT TGTCTTCAAG TCCAGAAGTG ACCACCATCC AGTGCCTGCA AGAACGTTGT 
ATACAGAAGT TTAAAGCTAC TCTTGAGTTA AAAGACCCAG TAGTACAAAT AAAGTGCTAT 
CAGCTACTGC TTTCCATTTT CCAGTATCCA AACCCAGCAG TATCCTACCC TTATATTCAT 
TCATTAGCAT CATCTATTGT GAGAAAACTG CAAGAGACAG ATAAAGTAAA ACCAGAAGAT 
GCTGCTGAGC TTCAGGTGAT TCAAGAAGGT ATTAAGGTCT TAGAAGCCCT AGTAGGGATT 
GCGGAAGAAC AGCACCGTGC TCACTTGGTG GCCGACATTC TCCCTATTCT TATTTCCTTT 
CTTTTGGATG AAAACACTCT GGGCTCAGCA CCCACCACAA TGAGAAATTT ACATGACTTT 
GCTTTGCAGA ATCTGATGCA GATTGGTCCC CAGTATTCAT CAACTTTTAA AAATATTATG 
GCTTCTTCTC CCACAATGAA AGCCCGTCTT GAGGCAGCTA TAAAAGGCAA TCAGGAAAAT 
ATCAAAATCA AGACAACCCC AAAACATATC ACAAAACCTG GAAAAAATTC AAGTATTCAG 
TTGAAGACCA ATTTTTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012402435.1
CDS	196..6336
Translation	MELAHSLLLNEEAYNQLGEVQKAEFVFEWLKFLEKLLLATNKADIKEKQKSIIEQLISLLNSSPGPPTRKLLAENLAVLYSIGDTFSVYQTIDKCNELIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRLLGNTFIDTVGNILKAMKSAESQGRYEIMLSLQNILSGLGAAAAPCHRDIYKAARSCLTDRSMAVRCAAAKCLLELQNEASFMWSTDIDSVATLCFKSFEGSNYDVRIAVSKLLGTVLARAVMSKPGTATSRQNVRKVSLEEAMELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFISTLGGRWLERNFSILLSHILSLVSQFHPKAIQTQMDAICCRRCVSFILRTTIGSLLGEKAQIAAARDICQVIWKLKKTVDAVMSEVNLETRPGSTDVAASQHVLVCALQELGNLIYGLGTTAAPLLQDSSAGILDNVISVIVHPSISVRLAAAWCLHCIAVALPSYLTPLLDSCLERLTVLKSSPEAVTGFSFAVAALLGAVKYCPLGIPHGKGKIIVMLAEDLLCSAAQNSRLSVQRTQAGWLLIAALMTLGSAFIHHHLPRVLRLWKCVFPLSPKDLETERSRGDSFTWQVTLEGRAGAFCAIKSFVSHCNDLLTDEVIQRLLPPLPCAVNLLTQLSSIQKTYGSPLKTPSVVYKQRLYELLALLPPEIYAGSLCAILKELAADLTSSDTQMAASTFLLPPLCHQDDLLLLSPLLQDTDHRFIEEQLLLGNGVACGSLEYDPFSVYEHGIEGDSVPKPLPPMLSLISSAARLFGIVCAHVAESQRLLVLEQLLDKIKHTKASRQQVVQLHVMAAFSNFLKYVANSKGNLGSEEIRRPALTLLMGALESTNPLMRCTAAEGLARLAQVIGDGSFTAGLAQVSFEKLKSARDVVTRTGHSLALGSLHRYLGGVGSSQHLNACIGILYTLSQDGTSPDVQTWALHSLSLIIDSAGPLYHLHVESTLSLILMLLLTVPPTHAEVHQSLGRCLNVLITTLGPELQGSSTAVSTLRTSCLLGCAIMQDNPDCLVQAQAISCLQQLHMFAPRYVNLSSLVSCLCVNLCSSYLFLRRAVLACLRQIVQREAAEVSEHAVVLAKDSREDLIPDANIREVGLEGALLNLLDKESDQRLCQDIKETLSHMITSMAVGKLSFWLKLCKDVLASSADFTTVASVDTTQDDEIDKEDDASVLTYRNDEKCRPITNPRWATRVFAAECVCKIISQCENSDSAHFDIALAQEMKERDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLQTLLVVVRQFATIPEPEFPGHVILEQYQANVGAALRPAFTSDTPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSQLYNESASTMEILAVLKAWAEVYIIAVERQKKQTEKQKQTMKIVGSIEENARDVSSSIDGLLDLVQTDLGTLSKLWLAALQDFALLTLPSEFASQLPLEGGAFYTAETSENAKPHYHNSWALILHATALWLTSTGFIVADPDEGATNLSRPVTPTTMCQGSSGASVKSPEDVYTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGVELLNVLHRVILTRESPSIQLASLEVVRQVIYAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGRSLVFATLELCVCILVRQLPELNPKLTGSPGIKTTKAQILSDDGNKLVSAALTILSELPGVCSPEGSISVLPTILYLIIGVLRETAVRLPGGQLSYTVGAALQALKGILASPLARAEKSHTAWTDLLRSALTTILDCWDPDGTQQEFDEISLLTAITVFILSSSPEVTTIQCLQERCIQKFKATLELKDPVVQIKCYQLLLSIFQYPNPAVSYPYIHSLASSIVRKLQETDKVKPEDAAELQVIQEGIKVLEALVGIAEEQHRAHLVADILPILISFLLDENTLGSAPTTMRNLHDFALQNLMQIGPQYSSTFKNIMASSPTMKARLEAAIKGNQENIKIKTTPKHITKPGKNSSIQLKTNFF

Target ORF information:

RefSeq Version	XM_012546981.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012546981.1

ORF Insert Sequence:

ATGGAACTGG CTCACAGCTT ATTGCTGAAT GAAGAAGCAT ATAATCAACT TGGAGAAGTT 
CAGAAGGCAG AATTTGTTTT TGAATGGTTG AAATTTTTAG AGAAACTTTT GCTAGCAACC 
AACAAGGCTG ACATAAAGGA GAAACAGAAG AGTATTATTG AGCAGCTCAT ATCTCTATTA 
AATAGCTCTC CAGGGCCACC TACCCGAAAG CTTCTTGCTG AGAACCTAGC TGTTCTTTAT 
AGTATTGGAG ATACATTTTC TGTTTATCAA ACAATTGACA AATGTAATGA ACTCATTCGT 
AGCAAAGATG ACTCTCCAAG TTATCTTCCC ACAAAACTTG CTGCTGTGGT ATGTCTGGGT 
TCTTTGTATA AGAAGTTGGG GAGACTATTG GGTAACACCT TTATAGATAC AGTGGGGAAT 
ATACTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGTCGAT ATGAGATTAT GCTGAGTTTG 
CAAAATATAC TGAGTGGACT TGGAGCTGCT GCTGCCCCTT GTCACAGAGA CATTTATAAA 
GCTGCAAGGT CATGTTTGAC AGACAGGTCC ATGGCAGTTC GCTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAGAATGA AGCTAGCTTT ATGTGGAGTA CAGACATAGA CAGTGTTGCA 
ACTTTGTGTT TTAAGTCATT TGAAGGTTCC AACTATGATG TGCGAATAGC AGTATCAAAG 
CTCTTAGGTA CAGTGTTGGC TAGAGCTGTA ATGTCCAAAC CTGGAACAGC AACTTCCCGG 
CAAAATGTTC GCAAAGTTTC TCTGGAGGAA GCCATGGAGT TACTAGGAAC AGGGTTTCTA 
CGTGGGAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGT TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG TGTTACTCAG GCATATGTGG TGTTTATTTC AACACTAGGA 
GGACGTTGGT TAGAAAGAAA TTTTTCCATC TTACTCTCTC ATATCCTTAG CCTAGTATCA 
CAGTTTCACC CTAAAGCAAT CCAAACTCAG ATGGATGCTA TCTGTTGTCG TCGCTGTGTT 
TCATTTATCC TTCGAACTAC TATAGGAAGC CTTCTCGGGG AAAAAGCTCA AATTGCTGCT 
GCCAGGGATA TTTGTCAGGT CATCTGGAAA CTAAAGAAAA CTGTTGATGC TGTAATGAGT 
GAAGTTAATT TGGAAACTCG ACCTGGCTCC ACAGATGTTG CTGCCAGCCA GCATGTGCTA 
GTATGTGCTT TACAAGAACT TGGAAATCTA ATATATGGTC TTGGCACAAC AGCTGCACCT 
TTATTACAGG ATTCAAGTGC AGGGATTCTT GACAATGTCA TTTCAGTTAT TGTTCATCCC 
AGCATTTCAG TTCGATTAGC AGCAGCTTGG TGTTTACACT GCATTGCTGT AGCATTGCCT 
TCCTACCTAA CTCCACTTTT GGACAGTTGC CTTGAACGAC TTACTGTGCT CAAATCCTCA 
CCTGAAGCTG TGACTGGCTT TAGTTTTGCT GTGGCAGCTC TACTAGGAGC AGTGAAATAT 
TGCCCTTTAG GAATTCCACA TGGAAAAGGA AAGATAATTG TGATGTTAGC AGAGGATTTG 
TTATGTTCTG CTGCTCAGAA TAGTCGCCTT TCAGTTCAAC GAACACAAGC TGGATGGTTG 
CTGATAGCTG CCTTAATGAC ATTAGGCTCT GCATTTATTC ATCACCACCT TCCCCGGGTG 
CTAAGATTAT GGAAATGTGT TTTTCCACTA TCTCCTAAGG ATCTAGAAAC AGAAAGAAGT 
CGAGGAGACT CATTTACATG GCAGGTGACC TTGGAAGGTC GTGCAGGGGC CTTCTGTGCT 
ATCAAGAGCT TTGTTTCCCA CTGTAATGAT CTTCTTACTG ATGAAGTCAT TCAGAGACTT 
CTTCCTCCCC TTCCGTGTGC TGTTAATTTA TTAACTCAAC TTTCTTCAAT ACAGAAAACA 
TATGGAAGTC CTTTAAAAAC TCCTTCAGTG GTTTACAAAC AAAGACTTTA TGAACTATTG 
GCTTTATTAC CTCCTGAGAT CTATGCAGGA AGCCTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACCTGA CTTCTTCTGA TACCCAGATG GCAGCATCCA CTTTCTTACT TCCACCCCTC 
TGTCACCAAG ATGATCTTTT ATTACTAAGT CCTTTGCTAC AAGACACTGA CCACAGATTT 
ATTGAAGAAC AGCTCCTCCT TGGTAATGGT GTTGCTTGTG GAAGTCTAGA GTATGACCCA 
TTTTCAGTTT ATGAACATGG CATAGAAGGG GATTCAGTAC CTAAGCCCCT ACCTCCAATG 
TTGTCACTTA TCAGCTCTGC AGCCAGGCTT TTTGGAATTG TATGTGCCCA TGTAGCAGAA 
TCTCAAAGGC TCTTGGTATT GGAACAACTT TTGGACAAAA TAAAACACAC AAAAGCATCT 
CGTCAACAAG TAGTACAATT ACATGTTATG GCAGCATTTT CTAATTTTTT GAAGTATGTG 
GCCAACTCCA AAGGAAATTT AGGCTCAGAA GAAATTAGAA GACCTGCACT AACATTATTG 
ATGGGAGCCT TGGAAAGTAC CAACCCCTTA ATGCGTTGTA CTGCTGCAGA AGGTTTGGCT 
AGATTAGCCC AAGTGATTGG TGATGGCTCC TTCACTGCTG GATTAGCACA GGTTAGCTTT 
GAAAAACTGA AATCAGCTAG GGATGTGGTA ACCAGAACAG GACATTCTTT GGCTCTGGGA 
TCCTTACATA GATATTTAGG AGGAGTGGGC TCTTCTCAGC ACTTAAATGC TTGCATCGGA 
ATACTTTACA CTCTGTCACA GGATGGAACT TCTCCTGATG TACAGACATG GGCCCTACAT 
TCTCTCTCAT TGATAATTGA TTCTGCTGGT CCACTGTACC ATTTACATGT GGAATCTACC 
CTCTCTCTTA TACTAATGTT GTTGTTGACT GTTCCACCTA CTCATGCTGA AGTTCACCAA 
AGTCTTGGAC GATGTTTGAA TGTACTCATC ACCACATTGG GTCCAGAACT ACAAGGTAGC 
AGTACTGCAG TTTCTACTTT ACGGACATCC TGTCTTTTGG GGTGTGCAAT AATGCAGGAT 
AACCCAGATT GCCTTGTTCA AGCTCAAGCC ATCTCTTGTC TTCAACAACT TCATATGTTT 
GCCCCACGCT ATGTCAATTT ATCTAGTCTA GTCAGCTGTC TTTGTGTGAA TCTCTGTAGC 
TCCTACTTGT TTCTGAGAAG AGCAGTACTA GCTTGTTTAC GTCAAATTGT GCAAAGGGAA 
GCAGCTGAGG TTTCAGAACA TGCTGTTGTT CTTGCTAAGG ACAGCAGAGA AGACTTAATT 
CCAGATGCTA ATATCCGAGA AGTGGGTCTT GAGGGGGCAT TGTTGAACTT ACTAGACAAA 
GAATCTGATC AGAGATTGTG CCAGGATATC AAAGAGACCC TGAGTCATAT GATCACATCA 
ATGGCAGTGG GAAAGCTTTC TTTTTGGCTA AAACTTTGTA AAGATGTCCT TGCTTCATCA 
GCTGATTTTA CAACTGTTGC TTCTGTGGAT ACAACTCAGG ATGATGAAAT AGATAAAGAA 
GATGATGCTT CAGTCCTAAC CTACAGAAAT GATGAGAAAT GCCGGCCAAT TACAAATCCA 
CGATGGGCTA CTAGAGTTTT TGCTGCTGAA TGTGTGTGTA AAATAATCAG CCAGTGTGAG 
AATTCAGACA GTGCTCACTT TGACATTGCT TTGGCTCAAG AAATGAAAGA AAGGGATTCA 
CGAAATGATT TTTTGGTATT ACATCTTGCT GACTTGATCC GCATGGCTTT TATGGCTGCA 
ACAGACCACA GTGACCAGCT CCGTCTTTCT GGTCTTCAGA CACTGTTAGT AGTTGTTCGG 
CAATTTGCAA CTATTCCTGA ACCAGAATTC CCAGGTCATG TGATTCTGGA ACAGTATCAA 
GCCAATGTGG GAGCAGCACT TAGACCAGCT TTTACTTCAG ATACACCACC AGATGTAACT 
GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG ATAGCAAGTG GAGTTGTAAG TGATCTTAAT 
GATCTCCGAA GAGTTCATCA ATTGCTTGTA TCATCCTTGA CGAAAATACA GGCTGGAAAA 
GAAGCCTTGA GTCAATTATA TAACGAAAGT GCTTCTACTA TGGAAATCCT AGCTGTTCTC 
AAAGCCTGGG CAGAGGTTTA CATAATTGCT GTAGAAAGAC AGAAAAAACA AACTGAGAAA 
CAGAAGCAGA CTATGAAAAT TGTTGGCTCT ATAGAGGAAA ATGCCAGAGA TGTGTCATCT 
TCCATAGATG GACTTCTTGA CTTAGTCCAA ACAGATCTTG GAACATTAAG CAAACTCTGG 
CTTGCTGCTC TTCAAGATTT TGCTCTTCTA ACTTTGCCTT CAGAATTTGC TTCCCAACTT 
CCTCTTGAAG GTGGAGCTTT CTACACTGCA GAAACTAGTG AAAATGCAAA GCCACACTAT 
CATAATTCGT GGGCTCTTAT TCTTCATGCT ACAGCATTGT GGCTCACTAG CACAGGCTTC 
ATTGTAGCTG ACCCAGATGA AGGAGCAACT AATCTCTCAA GACCTGTAAC ACCGACTACA 
ATGTGTCAGG GTTCTTCTGG GGCATCAGTG AAATCCCCTG AGGATGTTTA TACAGACAGA 
TTTCACCTTA TCTTGGGAAT CAGTGTGGAA TTTCTCTGTT CCCTGCGTTC AGATGCAACC 
ATGGAAAGCA TCACTGCTTG TTTACATGCA CTGCAGGCTT TACTGGATGT TCCTTGGCCC 
AGATCTAAGA TTGGCAGTGA CCAGGACTTG GGAGTTGAGT TGTTGAATGT ACTCCATCGA 
GTAATACTGA CTAGAGAGTC ACCTTCCATT CAGTTAGCTT CACTTGAAGT TGTAAGACAG 
GTTATCTACG CAGCCCAAGA ACATGTAAAG GAAAAGAGGA GAAGTGCAGA AGTTGATGAT 
GGAGCTGCTG AAAAGGAAAC CCTGCCAGAA TTTGGAGAAG GAAAGGATAC GGGAGGACTG 
GTACCTGGAA GATCTCTGGT GTTTGCAACA TTGGAGCTAT GTGTATGCAT TTTGGTCCGG 
CAACTTCCAG AGTTAAATCC TAAACTGACC GGTAGTCCAG GAATAAAAAC TACAAAAGCA 
CAGATTCTTT CAGATGATGG AAATAAGTTG GTTTCAGCAG CATTAACTAT CCTCTCAGAA 
CTTCCTGGAG TATGCTCTCC CGAAGGAAGC ATTTCAGTTC TCCCTACTAT TCTGTACCTT 
ATAATTGGGG TCCTCCGAGA AACTGCTGTG AGGTTACCTG GAGGACAGTT ATCTTATACT 
GTTGGAGCTG CTCTACAAGC CTTGAAAGGA ATATTGGCAT CTCCCTTGGC TCGAGCAGAG 
AAGAGTCACA CTGCTTGGAC TGACCTTCTT CGAAGTGCTT TGACAACAAT CCTAGACTGC 
TGGGATCCAG ATGGAACACA GCAAGAGTTT GATGAAATCA GCCTGCTTAC TGCCATAACA 
GTATTTATCT TGTCTTCAAG TCCAGAAGTG ACCACCATCC AGTGCCTGCA AGAACGTTGT 
ATACAGAAGT TTAAAGCTAC TCTTGAGTTA AAAGACCCAG TAGTACAAAT AAAGTGCTAT 
CAGCTACTGC TTTCCATTTT CCAGTATCCA AACCCAGCAG TATCCTACCC TTATATTCAT 
TCATTAGCAT CATCTATTGT GAGAAAACTG CAAGAGACAG ATAAAGTAAA ACCAGAAGAT 
GCTGCTGAGC TTCAGGTGAT TCAAGAAGGT ATTAAGGTCT TAGAAGCCCT AGTAGGGATT 
GCGGAAGAAC AGCACCGTGC TCACTTGGTG GCCGACATTC TCCCTATTCT TATTTCCTTT 
CTTTTGGATG AAAACACTCT GGGCTCAGCA CCCACCACAA TGAGAAATTT ACATGACTTT 
GCTTTGCAGA ATCTGATGCA GATTGGTCCC CAGTATTCAT CAACTTTTAA AAATATTATG 
GCTTCTTCTC CCACAATGAA AGCCCGTCTT GAGGCAGCTA TAAAAGGCAA TCAGGAAAAT 
ATCAAAATCA AGACAACCCC AAAACATATC ACAAAACCTG GAAAAAATTC AAGTATTCAG 
TTGAAGACCA ATTTTTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_031952257.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031808117.1 Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X2, mRNA.

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CloneID	OSa225025
Clone ID Related Accession (Same CDS sequence)	XM_031952257.1 , XM_031952256.1 , XM_031952259.1 , XM_031952260.1
Accession Version	XM_031952257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG CTCACAGCTT ATTGCTGAAT GAAGAAGCAT ATAATCAACT TGGAGAAGTT 
CAGAAGGCAG AATTTGTTTT TGAATGGTTG AAATTTTTAG AGAAACTTTT GCTAGCAACC 
AACAAGGCTG ACATAAAGGA GAAACAGAAG AGTATTATTG AGCAGCTCAT ATCTCTATTA 
AATAGCTCTC CAGGGCCACC TACCCGAAAG CTTCTTGCTG AGAACCTAGC TGTTCTTTAT 
AGTATTGGAG ATACATTTTC TGTTTATCAA ACAATTGACA AATGTAATGA ACTCATTCGT 
AGCAAAGATG ACTCTCCAAG TTATCTTCCC ACAAAACTTG CTGCTGTGGT ATGTCTGGGT 
TCTTTGTATA AGAAGTTGGG GAGACTATTG GGTAACACCT TTATAGATAC AGTGGGGAAT 
ATACTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGTCGAT ATGAGATTAT GCTGAGTTTG 
CAAAATATAC TGAGTGGACT TGGAGCTGCT GCTGCCCCTT GTCACAGAGA CATTTATAAA 
GCTGCAAGGT CATGTTTGAC AGACAGGTCC ATGGCAGTTC GCTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAGAATGA AGCTAGCTTT ATGTGGAGTA CAGACATAGA CAGTGTTGCA 
ACTTTGTGTT TTAAGTCATT TGAAGGTTCC AACTATGATG TGCGAATAGC AGTATCAAAG 
CTCTTAGGTA CAGTGTTGGC TAGAGCTGTA ATGTCCAAAC CTGGAACAGC AACTTCCCGG 
CAAAATGTTC GCAAAGTTTC TCTGGAGGAA GCCATGGAGT TACTAGGAAC AGGGTTTCTA 
CGTGGGAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGT TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG TGTTACTCAG GCATATGTGG TGTTTATTTC AACACTAGGA 
GGACGTTGGT TAGAAAGAAA TTTTTCCATC TTACTCTCTC ATATCCTTAG CCTAGTATCA 
CAGTTTCACC CTAAAGCAAT CCAAACTCAG ATGGATGCTA TCTGTTGTCG TCGCTGTGTT 
TCATTTATCC TTCGAACTAC TATAGGAAGC CTTCTCGGGG AAAAAGCTCA AATTGCTGCT 
GCCAGGGATA TTTGTCAGGT CATCTGGAAA CTAAAGAAAA CTGTTGATGC TGTAATGAGT 
GAAGTTAATT TGGAAACTCG ACCTGGCTCC ACAGATGTTG CTGCCAGCCA GCATGTGCTA 
GTATGTGCTT TACAAGAACT TGGAAATCTA ATATATGGTC TTGGCACAAC AGCTGCACCT 
TTATTACAGG ATTCAAGTGC AGGGATTCTT GACAATGTCA TTTCAGTTAT TGTTCATCCC 
AGCATTTCAG TTCGATTAGC AGCAGCTTGG TGTTTACACT GCATTGCTGT AGCATTGCCT 
TCCTACCTAA CTCCACTTTT GGACAGTTGC CTTGAACGAC TTACTGTGCT CAAATCCTCA 
CCTGAAGCTG TGACTGGCTT TAGTTTTGCT GTGGCAGCTC TACTAGGAGC AGTGAAATAT 
TGCCCTTTAG GAATTCCACA TGGAAAAGGA AAGATAATTG TGATGTTAGC AGAGGATTTG 
TTATGTTCTG CTGCTCAGAA TAGTCGCCTT TCAGTTCAAC GAACACAAGC TGGATGGTTG 
CTGATAGCTG CCTTAATGAC ATTAGGCTCT GCATTTATTC ATCACCACCT TCCCCGGGTG 
CTAAGATTAT GGAAATGTGT TTTTCCACTA TCTCCTAAGG ATCTAGAAAC AGAAAGAAGT 
CGAGGAGACT CATTTACATG GCAGGTGACC TTGGAAGGTC GTGCAGGGGC CTTCTGTGCT 
ATCAAGAGCT TTGTTTCCCA CTGTAATGAT CTTCTTACTG ATGAAGTCAT TCAGAGACTT 
CTTCCTCCCC TTCCGTGTGC TGTTAATTTA TTAACTCAAC TTTCTTCAAT ACAGAAAACA 
TATGGAAGTC CTTTAAAAAC TCCTTCAGTG GTTTACAAAC AAAGACTTTA TGAACTATTG 
GCTTTATTAC CTCCTGAGAT CTATGCAGGA AGCCTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACCTGA CTTCTTCTGA TACCCAGATG GCAGCATCCA CTTTCTTACT TCCACCCCTC 
TGTCACCAAG ATGATCTTTT ATTACTAAGT CCTTTGCTAC AAGACACTGA CCACAGATTT 
ATTGAAGAAC AGCTCCTCCT TGGTAATGGT GTTGCTTGTG GAAGTCTAGA GTATGACCCA 
TTTTCAGTTT ATGAACATGG CATAGAAGGG GATTCAGTAC CTAAGCCCCT ACCTCCAATG 
TTGTCACTTA TCAGCTCTGC AGCCAGGCTT TTTGGAATTG TATGTGCCCA TGTAGCAGAA 
TCTCAAAGGC TCTTGGTATT GGAACAACTT TTGGACAAAA TAAAACACAC AAAAGCATCT 
CGTCAACAAG TAGTACAATT ACATGTTATG GCAGCATTTT CTAATTTTTT GAAGTATGTG 
GCCAACTCCA AAGGAAATTT AGGCTCAGAA GAAATTAGAA GACCTGCACT AACATTATTG 
ATGGGAGCCT TGGAAAGTAC CAACCCCTTA ATGCGTTGTA CTGCTGCAGA AGGTTTGGCT 
AGATTAGCCC AAGTGATTGG TGATGGCTCC TTCACTGCTG GATTAGCACA GGTTAGCTTT 
GAAAAACTGA AATCAGCTAG GGATGTGGTA ACCAGAACAG GACATTCTTT GGCTCTGGGA 
TCCTTACATA GATATTTAGG AGGAGTGGGC TCTTCTCAGC ACTTAAATGC TTGCATCGGA 
ATACTTTACA CTCTGTCACA GGATGGAACT TCTCCTGATG TACAGACATG GGCCCTACAT 
TCTCTCTCAT TGATAATTGA TTCTGCTGGT CCACTGTACC ATTTACATGT GGAATCTACC 
CTCTCTCTTA TACTAATGTT GTTGTTGACT GTTCCACCTA CTCATGCTGA AGTTCACCAA 
AGTCTTGGAC GATGTTTGAA TGTACTCATC ACCACATTGG GTCCAGAACT ACAAGGTAGC 
AGTACTGCAG TTTCTACTTT ACGGACATCC TGTCTTTTGG GGTGTGCAAT AATGCAGGAT 
AACCCAGATT GCCTTGTTCA AGCTCAAGCC ATCTCTTGTC TTCAACAACT TCATATGTTT 
GCCCCACGCT ATGTCAATTT ATCTAGTCTA GTCAGCTGTC TTTGTGAGAT CTTGTTGGAT 
AATTCTGTGT TGGTGAATCT CTGTAGCTCC TACTTGTTTC TGAGAAGAGC AGTACTAGCT 
TGTTTACGTC AAATTGTGCA AAGGGAAGCA GCTGAGGTTT CAGAACATGC TGTTGTTCTT 
GCTAAGGACA GCAGAGAAGA CTTAATTCCA GATGCTAATA TCCGAGAAGT GGGTCTTGAG 
GGGGCATTGT TGAACTTACT AGACAAAGAA TCTGATCAGA GATTGTGCCA GGATATCAAA 
GAGACCCTGA GTCATATGAT CACATCAATG GCAGTGGGAA AGCTTTCTTT TTGGCTAAAA 
CTTTGTAAAG ATGTCCTTGC TTCATCAGCT GATTTTACAA CTGTTGCTTC TGTGGATACA 
ACTCAGGATG ATGAAATAGA TAAAGAAGAT GATGCTTCAG TCCTAACCTA CAGAAATGAT 
GAGAAATGCC GGCCAATTAC AAATCCACGA TGGGCTACTA GAGTTTTTGC TGCTGAATGT 
GTGTGTAAAA TAATCAGCCA GTGTGAGAAT TCAGACAGTG CTCACTTTGA CATTGCTTTG 
GCTCAAGAAA TGAAAGAAAG GGATTCACGA AATGATTTTT TGGTATTACA TCTTGCTGAC 
TTGATCCGCA TGGCTTTTAT GGCTGCAACA GACCACAGTG ACCAGCTCCG TCTTTCTGGT 
CTTCAGACAC TGTTAGTAGT TGTTCGGCAA TTTGCAACTA TTCCTGAACC AGAATTCCCA 
GGTCATGTGA TTCTGGAACA GTATCAAGCC AATGTGGGAG CAGCACTTAG ACCAGCTTTT 
ACTTCAGATA CACCACCAGA TGTAACTGCC AAAGCATGTC AGGTTTGCAG TGCCTGGATA 
GCAAGTGGAG TTGTAAGTGA TCTTAATGAT CTCCGAAGAG TTCATCAATT GCTTGTATCA 
TCCTTGACGA AAATACAGGC TGGAAAAGAA GCCTTGAGTC AATTATATAA CGAAAGTGCT 
TCTACTATGG AAATCCTAGC TGTTCTCAAA GCCTGGGCAG AGGTTTACAT AATTGCTGTA 
GAAAGACAGA AAAAACAAAC TGAGAAACAG AAGCAGACTA TGAAAATTGT TGGCTCTATA 
GAGGAAAATG CCAGAGATGT GTCATCTTCC ATAGATGGAC TTCTTGACTT AGTCCAAACA 
GATCTTGGAA CATTAAGCAA ACTCTGGCTT GCTGCTCTTC AAGATTTTGC TCTTCTAACT 
TTGCCTTCAG AATTTGCTTC CCAACTTCCT CTTGAAGGTG GAGCTTTCTA CACTGCAGAA 
ACTAGTGAAA ATGCAAAGCC ACACTATCAT AATTCGTGGG CTCTTATTCT TCATGCTACA 
GCATTGTGGC TCACTAGCAC AGGCTTCATT GTAGCTGACC CAGATGAAGG AGCAACTAAT 
CTCTCAAGAC CTGTAACACC GACTACAATG TGTCAGGGTT CTTCTGGGGC ATCAGTGAAA 
TCCCCTGAGG ATGTTTATAC AGACAGATTT CACCTTATCT TGGGAATCAG TGTGGAATTT 
CTCTGTTCCC TGCGTTCAGA TGCAACCATG GAAAGCATCA CTGCTTGTTT ACATGCACTG 
CAGGCTTTAC TGGATGTTCC TTGGCCCAGA TCTAAGATTG GCAGTGACCA GGACTTGGGA 
GTTGAGTTGT TGAATGTACT CCATCGAGTA ATACTGACTA GAGAGTCACC TTCCATTCAG 
TTAGCTTCAC TTGAAGTTGT AAGACAGGTT ATCTACGCAG CCCAAGAACA TGTAAAGGAA 
AAGAGGAGAA GTGCAGAAGT TGATGATGGA GCTGCTGAAA AGGAAACCCT GCCAGAATTT 
GGAGAAGGAA AGGATACGGG AGGACTGGTA CCTGGAAGAT CTCTGGTGTT TGCAACATTG 
GAGCTATGTG TATGCATTTT GGTCCGGCAA CTTCCAGAGT TAAATCCTAA ACTGACCGGT 
AGTCCAGGAA TAAAAACTAC AAAAGCACAG ATTCTTTCAG ATGATGGAAA TAAGTTGGTT 
TCAGCAGCAT TAACTATCCT CTCAGAACTT CCTGGAGTAT GCTCTCCCGA AGGAAGCATT 
TCAGTTCTCC CTACTATTCT GTACCTTATA ATTGGGGTCC TCCGAGAAAC TGCTGTGAGG 
TTACCTGGAG GACAGTTATC TTATACTGTT GGAGCTGCTC TACAAGCCTT GAAAGGAATA 
TTGGCATCTC CCTTGGCTCG AGCAGAGAAG AGTCACACTG CTTGGACTGA CCTTCTTCGA 
AGTGCTTTGA CAACAATCCT AGACTGCTGG GATCCAGATG GAACACAGCA AGAGTTTGAT 
GAAATCAGCC TGCTTACTGC CATAACAGTA TTTATCTTGT CTTCAAGTCC AGAAGTGACC 
ACCATCCAGT GCCTGCAAGA ACGTTGTATA CAGAAGTTTA AAGCTACTCT TGAGTTAAAA 
GACCCAGTAG TACAAATAAA GTGCTATCAG CTACTGCTTT CCATTTTCCA GTATCCAAAC 
CCAGCAGTAT CCTACCCTTA TATTCATTCA TTAGCATCAT CTATTGTGAG AAAACTGCAA 
GAGACAGATA AAGTAAAACC AGAAGATGCT GCTGAGCTTC AGGTGATTCA AGAAGGTATT 
AAGGTCTTAG AAGCCCTAGT AGGGATTGCG GAAGAACAGC ACCGTGCTCA CTTGGTGGCC 
GACATTCTCC CTATTCTTAT TTCCTTTCTT TTGGATGAAA ACACTCTGGG CTCAGCACCC 
ACCACAATGA GAAATTTACA TGACTTTGCT TTGCAGAATC TGATGCAGAT TGGTCCCCAG 
TATTCATCAA CTTTTAAAAA TATTATGGCT TCTTCTCCCA CAATGAAAGC CCGTCTTGAG 
GCAGCTATAA AAGGCAATCA GGAAAATATC AAAATCAAGA CAACCCCAAA ACATATCACA 
AAACCTGGAA AAAATTCAAG TATTCAGTTG AAGACCAATT TTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031808117.1
CDS	303..6470
Translation	MELAHSLLLNEEAYNQLGEVQKAEFVFEWLKFLEKLLLATNKADIKEKQKSIIEQLISLLNSSPGPPTRKLLAENLAVLYSIGDTFSVYQTIDKCNELIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRLLGNTFIDTVGNILKAMKSAESQGRYEIMLSLQNILSGLGAAAAPCHRDIYKAARSCLTDRSMAVRCAAAKCLLELQNEASFMWSTDIDSVATLCFKSFEGSNYDVRIAVSKLLGTVLARAVMSKPGTATSRQNVRKVSLEEAMELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFISTLGGRWLERNFSILLSHILSLVSQFHPKAIQTQMDAICCRRCVSFILRTTIGSLLGEKAQIAAARDICQVIWKLKKTVDAVMSEVNLETRPGSTDVAASQHVLVCALQELGNLIYGLGTTAAPLLQDSSAGILDNVISVIVHPSISVRLAAAWCLHCIAVALPSYLTPLLDSCLERLTVLKSSPEAVTGFSFAVAALLGAVKYCPLGIPHGKGKIIVMLAEDLLCSAAQNSRLSVQRTQAGWLLIAALMTLGSAFIHHHLPRVLRLWKCVFPLSPKDLETERSRGDSFTWQVTLEGRAGAFCAIKSFVSHCNDLLTDEVIQRLLPPLPCAVNLLTQLSSIQKTYGSPLKTPSVVYKQRLYELLALLPPEIYAGSLCAILKELAADLTSSDTQMAASTFLLPPLCHQDDLLLLSPLLQDTDHRFIEEQLLLGNGVACGSLEYDPFSVYEHGIEGDSVPKPLPPMLSLISSAARLFGIVCAHVAESQRLLVLEQLLDKIKHTKASRQQVVQLHVMAAFSNFLKYVANSKGNLGSEEIRRPALTLLMGALESTNPLMRCTAAEGLARLAQVIGDGSFTAGLAQVSFEKLKSARDVVTRTGHSLALGSLHRYLGGVGSSQHLNACIGILYTLSQDGTSPDVQTWALHSLSLIIDSAGPLYHLHVESTLSLILMLLLTVPPTHAEVHQSLGRCLNVLITTLGPELQGSSTAVSTLRTSCLLGCAIMQDNPDCLVQAQAISCLQQLHMFAPRYVNLSSLVSCLCEILLDNSVLVNLCSSYLFLRRAVLACLRQIVQREAAEVSEHAVVLAKDSREDLIPDANIREVGLEGALLNLLDKESDQRLCQDIKETLSHMITSMAVGKLSFWLKLCKDVLASSADFTTVASVDTTQDDEIDKEDDASVLTYRNDEKCRPITNPRWATRVFAAECVCKIISQCENSDSAHFDIALAQEMKERDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLQTLLVVVRQFATIPEPEFPGHVILEQYQANVGAALRPAFTSDTPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSQLYNESASTMEILAVLKAWAEVYIIAVERQKKQTEKQKQTMKIVGSIEENARDVSSSIDGLLDLVQTDLGTLSKLWLAALQDFALLTLPSEFASQLPLEGGAFYTAETSENAKPHYHNSWALILHATALWLTSTGFIVADPDEGATNLSRPVTPTTMCQGSSGASVKSPEDVYTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGVELLNVLHRVILTRESPSIQLASLEVVRQVIYAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGRSLVFATLELCVCILVRQLPELNPKLTGSPGIKTTKAQILSDDGNKLVSAALTILSELPGVCSPEGSISVLPTILYLIIGVLRETAVRLPGGQLSYTVGAALQALKGILASPLARAEKSHTAWTDLLRSALTTILDCWDPDGTQQEFDEISLLTAITVFILSSSPEVTTIQCLQERCIQKFKATLELKDPVVQIKCYQLLLSIFQYPNPAVSYPYIHSLASSIVRKLQETDKVKPEDAAELQVIQEGIKVLEALVGIAEEQHRAHLVADILPILISFLLDENTLGSAPTTMRNLHDFALQNLMQIGPQYSSTFKNIMASSPTMKARLEAAIKGNQENIKIKTTPKHITKPGKNSSIQLKTNFF

Target ORF information:

RefSeq Version	XM_031952257.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031952257.1

ORF Insert Sequence:

ATGGAACTGG CTCACAGCTT ATTGCTGAAT GAAGAAGCAT ATAATCAACT TGGAGAAGTT 
CAGAAGGCAG AATTTGTTTT TGAATGGTTG AAATTTTTAG AGAAACTTTT GCTAGCAACC 
AACAAGGCTG ACATAAAGGA GAAACAGAAG AGTATTATTG AGCAGCTCAT ATCTCTATTA 
AATAGCTCTC CAGGGCCACC TACCCGAAAG CTTCTTGCTG AGAACCTAGC TGTTCTTTAT 
AGTATTGGAG ATACATTTTC TGTTTATCAA ACAATTGACA AATGTAATGA ACTCATTCGT 
AGCAAAGATG ACTCTCCAAG TTATCTTCCC ACAAAACTTG CTGCTGTGGT ATGTCTGGGT 
TCTTTGTATA AGAAGTTGGG GAGACTATTG GGTAACACCT TTATAGATAC AGTGGGGAAT 
ATACTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGTCGAT ATGAGATTAT GCTGAGTTTG 
CAAAATATAC TGAGTGGACT TGGAGCTGCT GCTGCCCCTT GTCACAGAGA CATTTATAAA 
GCTGCAAGGT CATGTTTGAC AGACAGGTCC ATGGCAGTTC GCTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAGAATGA AGCTAGCTTT ATGTGGAGTA CAGACATAGA CAGTGTTGCA 
ACTTTGTGTT TTAAGTCATT TGAAGGTTCC AACTATGATG TGCGAATAGC AGTATCAAAG 
CTCTTAGGTA CAGTGTTGGC TAGAGCTGTA ATGTCCAAAC CTGGAACAGC AACTTCCCGG 
CAAAATGTTC GCAAAGTTTC TCTGGAGGAA GCCATGGAGT TACTAGGAAC AGGGTTTCTA 
CGTGGGAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGT TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG TGTTACTCAG GCATATGTGG TGTTTATTTC AACACTAGGA 
GGACGTTGGT TAGAAAGAAA TTTTTCCATC TTACTCTCTC ATATCCTTAG CCTAGTATCA 
CAGTTTCACC CTAAAGCAAT CCAAACTCAG ATGGATGCTA TCTGTTGTCG TCGCTGTGTT 
TCATTTATCC TTCGAACTAC TATAGGAAGC CTTCTCGGGG AAAAAGCTCA AATTGCTGCT 
GCCAGGGATA TTTGTCAGGT CATCTGGAAA CTAAAGAAAA CTGTTGATGC TGTAATGAGT 
GAAGTTAATT TGGAAACTCG ACCTGGCTCC ACAGATGTTG CTGCCAGCCA GCATGTGCTA 
GTATGTGCTT TACAAGAACT TGGAAATCTA ATATATGGTC TTGGCACAAC AGCTGCACCT 
TTATTACAGG ATTCAAGTGC AGGGATTCTT GACAATGTCA TTTCAGTTAT TGTTCATCCC 
AGCATTTCAG TTCGATTAGC AGCAGCTTGG TGTTTACACT GCATTGCTGT AGCATTGCCT 
TCCTACCTAA CTCCACTTTT GGACAGTTGC CTTGAACGAC TTACTGTGCT CAAATCCTCA 
CCTGAAGCTG TGACTGGCTT TAGTTTTGCT GTGGCAGCTC TACTAGGAGC AGTGAAATAT 
TGCCCTTTAG GAATTCCACA TGGAAAAGGA AAGATAATTG TGATGTTAGC AGAGGATTTG 
TTATGTTCTG CTGCTCAGAA TAGTCGCCTT TCAGTTCAAC GAACACAAGC TGGATGGTTG 
CTGATAGCTG CCTTAATGAC ATTAGGCTCT GCATTTATTC ATCACCACCT TCCCCGGGTG 
CTAAGATTAT GGAAATGTGT TTTTCCACTA TCTCCTAAGG ATCTAGAAAC AGAAAGAAGT 
CGAGGAGACT CATTTACATG GCAGGTGACC TTGGAAGGTC GTGCAGGGGC CTTCTGTGCT 
ATCAAGAGCT TTGTTTCCCA CTGTAATGAT CTTCTTACTG ATGAAGTCAT TCAGAGACTT 
CTTCCTCCCC TTCCGTGTGC TGTTAATTTA TTAACTCAAC TTTCTTCAAT ACAGAAAACA 
TATGGAAGTC CTTTAAAAAC TCCTTCAGTG GTTTACAAAC AAAGACTTTA TGAACTATTG 
GCTTTATTAC CTCCTGAGAT CTATGCAGGA AGCCTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACCTGA CTTCTTCTGA TACCCAGATG GCAGCATCCA CTTTCTTACT TCCACCCCTC 
TGTCACCAAG ATGATCTTTT ATTACTAAGT CCTTTGCTAC AAGACACTGA CCACAGATTT 
ATTGAAGAAC AGCTCCTCCT TGGTAATGGT GTTGCTTGTG GAAGTCTAGA GTATGACCCA 
TTTTCAGTTT ATGAACATGG CATAGAAGGG GATTCAGTAC CTAAGCCCCT ACCTCCAATG 
TTGTCACTTA TCAGCTCTGC AGCCAGGCTT TTTGGAATTG TATGTGCCCA TGTAGCAGAA 
TCTCAAAGGC TCTTGGTATT GGAACAACTT TTGGACAAAA TAAAACACAC AAAAGCATCT 
CGTCAACAAG TAGTACAATT ACATGTTATG GCAGCATTTT CTAATTTTTT GAAGTATGTG 
GCCAACTCCA AAGGAAATTT AGGCTCAGAA GAAATTAGAA GACCTGCACT AACATTATTG 
ATGGGAGCCT TGGAAAGTAC CAACCCCTTA ATGCGTTGTA CTGCTGCAGA AGGTTTGGCT 
AGATTAGCCC AAGTGATTGG TGATGGCTCC TTCACTGCTG GATTAGCACA GGTTAGCTTT 
GAAAAACTGA AATCAGCTAG GGATGTGGTA ACCAGAACAG GACATTCTTT GGCTCTGGGA 
TCCTTACATA GATATTTAGG AGGAGTGGGC TCTTCTCAGC ACTTAAATGC TTGCATCGGA 
ATACTTTACA CTCTGTCACA GGATGGAACT TCTCCTGATG TACAGACATG GGCCCTACAT 
TCTCTCTCAT TGATAATTGA TTCTGCTGGT CCACTGTACC ATTTACATGT GGAATCTACC 
CTCTCTCTTA TACTAATGTT GTTGTTGACT GTTCCACCTA CTCATGCTGA AGTTCACCAA 
AGTCTTGGAC GATGTTTGAA TGTACTCATC ACCACATTGG GTCCAGAACT ACAAGGTAGC 
AGTACTGCAG TTTCTACTTT ACGGACATCC TGTCTTTTGG GGTGTGCAAT AATGCAGGAT 
AACCCAGATT GCCTTGTTCA AGCTCAAGCC ATCTCTTGTC TTCAACAACT TCATATGTTT 
GCCCCACGCT ATGTCAATTT ATCTAGTCTA GTCAGCTGTC TTTGTGAGAT CTTGTTGGAT 
AATTCTGTGT TGGTGAATCT CTGTAGCTCC TACTTGTTTC TGAGAAGAGC AGTACTAGCT 
TGTTTACGTC AAATTGTGCA AAGGGAAGCA GCTGAGGTTT CAGAACATGC TGTTGTTCTT 
GCTAAGGACA GCAGAGAAGA CTTAATTCCA GATGCTAATA TCCGAGAAGT GGGTCTTGAG 
GGGGCATTGT TGAACTTACT AGACAAAGAA TCTGATCAGA GATTGTGCCA GGATATCAAA 
GAGACCCTGA GTCATATGAT CACATCAATG GCAGTGGGAA AGCTTTCTTT TTGGCTAAAA 
CTTTGTAAAG ATGTCCTTGC TTCATCAGCT GATTTTACAA CTGTTGCTTC TGTGGATACA 
ACTCAGGATG ATGAAATAGA TAAAGAAGAT GATGCTTCAG TCCTAACCTA CAGAAATGAT 
GAGAAATGCC GGCCAATTAC AAATCCACGA TGGGCTACTA GAGTTTTTGC TGCTGAATGT 
GTGTGTAAAA TAATCAGCCA GTGTGAGAAT TCAGACAGTG CTCACTTTGA CATTGCTTTG 
GCTCAAGAAA TGAAAGAAAG GGATTCACGA AATGATTTTT TGGTATTACA TCTTGCTGAC 
TTGATCCGCA TGGCTTTTAT GGCTGCAACA GACCACAGTG ACCAGCTCCG TCTTTCTGGT 
CTTCAGACAC TGTTAGTAGT TGTTCGGCAA TTTGCAACTA TTCCTGAACC AGAATTCCCA 
GGTCATGTGA TTCTGGAACA GTATCAAGCC AATGTGGGAG CAGCACTTAG ACCAGCTTTT 
ACTTCAGATA CACCACCAGA TGTAACTGCC AAAGCATGTC AGGTTTGCAG TGCCTGGATA 
GCAAGTGGAG TTGTAAGTGA TCTTAATGAT CTCCGAAGAG TTCATCAATT GCTTGTATCA 
TCCTTGACGA AAATACAGGC TGGAAAAGAA GCCTTGAGTC AATTATATAA CGAAAGTGCT 
TCTACTATGG AAATCCTAGC TGTTCTCAAA GCCTGGGCAG AGGTTTACAT AATTGCTGTA 
GAAAGACAGA AAAAACAAAC TGAGAAACAG AAGCAGACTA TGAAAATTGT TGGCTCTATA 
GAGGAAAATG CCAGAGATGT GTCATCTTCC ATAGATGGAC TTCTTGACTT AGTCCAAACA 
GATCTTGGAA CATTAAGCAA ACTCTGGCTT GCTGCTCTTC AAGATTTTGC TCTTCTAACT 
TTGCCTTCAG AATTTGCTTC CCAACTTCCT CTTGAAGGTG GAGCTTTCTA CACTGCAGAA 
ACTAGTGAAA ATGCAAAGCC ACACTATCAT AATTCGTGGG CTCTTATTCT TCATGCTACA 
GCATTGTGGC TCACTAGCAC AGGCTTCATT GTAGCTGACC CAGATGAAGG AGCAACTAAT 
CTCTCAAGAC CTGTAACACC GACTACAATG TGTCAGGGTT CTTCTGGGGC ATCAGTGAAA 
TCCCCTGAGG ATGTTTATAC AGACAGATTT CACCTTATCT TGGGAATCAG TGTGGAATTT 
CTCTGTTCCC TGCGTTCAGA TGCAACCATG GAAAGCATCA CTGCTTGTTT ACATGCACTG 
CAGGCTTTAC TGGATGTTCC TTGGCCCAGA TCTAAGATTG GCAGTGACCA GGACTTGGGA 
GTTGAGTTGT TGAATGTACT CCATCGAGTA ATACTGACTA GAGAGTCACC TTCCATTCAG 
TTAGCTTCAC TTGAAGTTGT AAGACAGGTT ATCTACGCAG CCCAAGAACA TGTAAAGGAA 
AAGAGGAGAA GTGCAGAAGT TGATGATGGA GCTGCTGAAA AGGAAACCCT GCCAGAATTT 
GGAGAAGGAA AGGATACGGG AGGACTGGTA CCTGGAAGAT CTCTGGTGTT TGCAACATTG 
GAGCTATGTG TATGCATTTT GGTCCGGCAA CTTCCAGAGT TAAATCCTAA ACTGACCGGT 
AGTCCAGGAA TAAAAACTAC AAAAGCACAG ATTCTTTCAG ATGATGGAAA TAAGTTGGTT 
TCAGCAGCAT TAACTATCCT CTCAGAACTT CCTGGAGTAT GCTCTCCCGA AGGAAGCATT 
TCAGTTCTCC CTACTATTCT GTACCTTATA ATTGGGGTCC TCCGAGAAAC TGCTGTGAGG 
TTACCTGGAG GACAGTTATC TTATACTGTT GGAGCTGCTC TACAAGCCTT GAAAGGAATA 
TTGGCATCTC CCTTGGCTCG AGCAGAGAAG AGTCACACTG CTTGGACTGA CCTTCTTCGA 
AGTGCTTTGA CAACAATCCT AGACTGCTGG GATCCAGATG GAACACAGCA AGAGTTTGAT 
GAAATCAGCC TGCTTACTGC CATAACAGTA TTTATCTTGT CTTCAAGTCC AGAAGTGACC 
ACCATCCAGT GCCTGCAAGA ACGTTGTATA CAGAAGTTTA AAGCTACTCT TGAGTTAAAA 
GACCCAGTAG TACAAATAAA GTGCTATCAG CTACTGCTTT CCATTTTCCA GTATCCAAAC 
CCAGCAGTAT CCTACCCTTA TATTCATTCA TTAGCATCAT CTATTGTGAG AAAACTGCAA 
GAGACAGATA AAGTAAAACC AGAAGATGCT GCTGAGCTTC AGGTGATTCA AGAAGGTATT 
AAGGTCTTAG AAGCCCTAGT AGGGATTGCG GAAGAACAGC ACCGTGCTCA CTTGGTGGCC 
GACATTCTCC CTATTCTTAT TTCCTTTCTT TTGGATGAAA ACACTCTGGG CTCAGCACCC 
ACCACAATGA GAAATTTACA TGACTTTGCT TTGCAGAATC TGATGCAGAT TGGTCCCCAG 
TATTCATCAA CTTTTAAAAA TATTATGGCT TCTTCTCCCA CAATGAAAGC CCGTCTTGAG 
GCAGCTATAA AAGGCAATCA GGAAAATATC AAAATCAAGA CAACCCCAAA ACATATCACA 
AAACCTGGAA AAAATTCAAG TATTCAGTTG AAGACCAATT TTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_031952256.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031808116.1 Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X1, mRNA.

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CloneID	OSa225025
Clone ID Related Accession (Same CDS sequence)	XM_031952257.1 , XM_031952256.1 , XM_031952259.1 , XM_031952260.1
Accession Version	XM_031952256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG CTCACAGCTT ATTGCTGAAT GAAGAAGCAT ATAATCAACT TGGAGAAGTT 
CAGAAGGCAG AATTTGTTTT TGAATGGTTG AAATTTTTAG AGAAACTTTT GCTAGCAACC 
AACAAGGCTG ACATAAAGGA GAAACAGAAG AGTATTATTG AGCAGCTCAT ATCTCTATTA 
AATAGCTCTC CAGGGCCACC TACCCGAAAG CTTCTTGCTG AGAACCTAGC TGTTCTTTAT 
AGTATTGGAG ATACATTTTC TGTTTATCAA ACAATTGACA AATGTAATGA ACTCATTCGT 
AGCAAAGATG ACTCTCCAAG TTATCTTCCC ACAAAACTTG CTGCTGTGGT ATGTCTGGGT 
TCTTTGTATA AGAAGTTGGG GAGACTATTG GGTAACACCT TTATAGATAC AGTGGGGAAT 
ATACTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGTCGAT ATGAGATTAT GCTGAGTTTG 
CAAAATATAC TGAGTGGACT TGGAGCTGCT GCTGCCCCTT GTCACAGAGA CATTTATAAA 
GCTGCAAGGT CATGTTTGAC AGACAGGTCC ATGGCAGTTC GCTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAGAATGA AGCTAGCTTT ATGTGGAGTA CAGACATAGA CAGTGTTGCA 
ACTTTGTGTT TTAAGTCATT TGAAGGTTCC AACTATGATG TGCGAATAGC AGTATCAAAG 
CTCTTAGGTA CAGTGTTGGC TAGAGCTGTA ATGTCCAAAC CTGGAACAGC AACTTCCCGG 
CAAAATGTTC GCAAAGTTTC TCTGGAGGAA GCCATGGAGT TACTAGGAAC AGGGTTTCTA 
CGTGGGAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGT TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG TGTTACTCAG GCATATGTGG TGTTTATTTC AACACTAGGA 
GGACGTTGGT TAGAAAGAAA TTTTTCCATC TTACTCTCTC ATATCCTTAG CCTAGTATCA 
CAGTTTCACC CTAAAGCAAT CCAAACTCAG ATGGATGCTA TCTGTTGTCG TCGCTGTGTT 
TCATTTATCC TTCGAACTAC TATAGGAAGC CTTCTCGGGG AAAAAGCTCA AATTGCTGCT 
GCCAGGGATA TTTGTCAGGT CATCTGGAAA CTAAAGAAAA CTGTTGATGC TGTAATGAGT 
GAAGTTAATT TGGAAACTCG ACCTGGCTCC ACAGATGTTG CTGCCAGCCA GCATGTGCTA 
GTATGTGCTT TACAAGAACT TGGAAATCTA ATATATGGTC TTGGCACAAC AGCTGCACCT 
TTATTACAGG ATTCAAGTGC AGGGATTCTT GACAATGTCA TTTCAGTTAT TGTTCATCCC 
AGCATTTCAG TTCGATTAGC AGCAGCTTGG TGTTTACACT GCATTGCTGT AGCATTGCCT 
TCCTACCTAA CTCCACTTTT GGACAGTTGC CTTGAACGAC TTACTGTGCT CAAATCCTCA 
CCTGAAGCTG TGACTGGCTT TAGTTTTGCT GTGGCAGCTC TACTAGGAGC AGTGAAATAT 
TGCCCTTTAG GAATTCCACA TGGAAAAGGA AAGATAATTG TGATGTTAGC AGAGGATTTG 
TTATGTTCTG CTGCTCAGAA TAGTCGCCTT TCAGTTCAAC GAACACAAGC TGGATGGTTG 
CTGATAGCTG CCTTAATGAC ATTAGGCTCT GCATTTATTC ATCACCACCT TCCCCGGGTG 
CTAAGATTAT GGAAATGTGT TTTTCCACTA TCTCCTAAGG ATCTAGAAAC AGAAAGAAGT 
CGAGGAGACT CATTTACATG GCAGGTGACC TTGGAAGGTC GTGCAGGGGC CTTCTGTGCT 
ATCAAGAGCT TTGTTTCCCA CTGTAATGAT CTTCTTACTG ATGAAGTCAT TCAGAGACTT 
CTTCCTCCCC TTCCGTGTGC TGTTAATTTA TTAACTCAAC TTTCTTCAAT ACAGAAAACA 
TATGGAAGTC CTTTAAAAAC TCCTTCAGTG GTTTACAAAC AAAGACTTTA TGAACTATTG 
GCTTTATTAC CTCCTGAGAT CTATGCAGGA AGCCTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACCTGA CTTCTTCTGA TACCCAGATG GCAGCATCCA CTTTCTTACT TCCACCCCTC 
TGTCACCAAG ATGATCTTTT ATTACTAAGT CCTTTGCTAC AAGACACTGA CCACAGATTT 
ATTGAAGAAC AGCTCCTCCT TGGTAATGGT GTTGCTTGTG GAAGTCTAGA GTATGACCCA 
TTTTCAGTTT ATGAACATGG CATAGAAGGG GATTCAGTAC CTAAGCCCCT ACCTCCAATG 
TTGTCACTTA TCAGCTCTGC AGCCAGGCTT TTTGGAATTG TATGTGCCCA TGTAGCAGAA 
TCTCAAAGGC TCTTGGTATT GGAACAACTT TTGGACAAAA TAAAACACAC AAAAGCATCT 
CGTCAACAAG TAGTACAATT ACATGTTATG GCAGCATTTT CTAATTTTTT GAAGTATGTG 
GCCAACTCCA AAGGAAATTT AGGCTCAGAA GAAATTAGAA GACCTGCACT AACATTATTG 
ATGGGAGCCT TGGAAAGTAC CAACCCCTTA ATGCGTTGTA CTGCTGCAGA AGGTTTGGCT 
AGATTAGCCC AAGTGATTGG TGATGGCTCC TTCACTGCTG GATTAGCACA GGTTAGCTTT 
GAAAAACTGA AATCAGCTAG GGATGTGGTA ACCAGAACAG GACATTCTTT GGCTCTGGGA 
TCCTTACATA GATATTTAGG AGGAGTGGGC TCTTCTCAGC ACTTAAATGC TTGCATCGGA 
ATACTTTACA CTCTGTCACA GGATGGAACT TCTCCTGATG TACAGACATG GGCCCTACAT 
TCTCTCTCAT TGATAATTGA TTCTGCTGGT CCACTGTACC ATTTACATGT GGAATCTACC 
CTCTCTCTTA TACTAATGTT GTTGTTGACT GTTCCACCTA CTCATGCTGA AGTTCACCAA 
AGTCTTGGAC GATGTTTGAA TGTACTCATC ACCACATTGG GTCCAGAACT ACAAGGTAGC 
AGTACTGCAG TTTCTACTTT ACGGACATCC TGTCTTTTGG GGTGTGCAAT AATGCAGGAT 
AACCCAGATT GCCTTGTTCA AGCTCAAGCC ATCTCTTGTC TTCAACAACT TCATATGTTT 
GCCCCACGCT ATGTCAATTT ATCTAGTCTA GTCAGCTGTC TTTGTGAGAT CTTGTTGGAT 
AATTCTGTGT TGGTGAATCT CTGTAGCTCC TACTTGTTTC TGAGAAGAGC AGTACTAGCT 
TGTTTACGTC AAATTGTGCA AAGGGAAGCA GCTGAGGTTT CAGAACATGC TGTTGTTCTT 
GCTAAGGACA GCAGAGAAGA CTTAATTCCA GATGCTAATA TCCGAGAAGT GGGTCTTGAG 
GGGGCATTGT TGAACTTACT AGACAAAGAA TCTGATCAGA GATTGTGCCA GGATATCAAA 
GAGACCCTGA GTCATATGAT CACATCAATG GCAGTGGGAA AGCTTTCTTT TTGGCTAAAA 
CTTTGTAAAG ATGTCCTTGC TTCATCAGCT GATTTTACAA CTGTTGCTTC TGTGGATACA 
ACTCAGGATG ATGAAATAGA TAAAGAAGAT GATGCTTCAG TCCTAACCTA CAGAAATGAT 
GAGAAATGCC GGCCAATTAC AAATCCACGA TGGGCTACTA GAGTTTTTGC TGCTGAATGT 
GTGTGTAAAA TAATCAGCCA GTGTGAGAAT TCAGACAGTG CTCACTTTGA CATTGCTTTG 
GCTCAAGAAA TGAAAGAAAG GGATTCACGA AATGATTTTT TGGTATTACA TCTTGCTGAC 
TTGATCCGCA TGGCTTTTAT GGCTGCAACA GACCACAGTG ACCAGCTCCG TCTTTCTGGT 
CTTCAGACAC TGTTAGTAGT TGTTCGGCAA TTTGCAACTA TTCCTGAACC AGAATTCCCA 
GGTCATGTGA TTCTGGAACA GTATCAAGCC AATGTGGGAG CAGCACTTAG ACCAGCTTTT 
ACTTCAGATA CACCACCAGA TGTAACTGCC AAAGCATGTC AGGTTTGCAG TGCCTGGATA 
GCAAGTGGAG TTGTAAGTGA TCTTAATGAT CTCCGAAGAG TTCATCAATT GCTTGTATCA 
TCCTTGACGA AAATACAGGC TGGAAAAGAA GCCTTGAGTC AATTATATAA CGAAAGTGCT 
TCTACTATGG AAATCCTAGC TGTTCTCAAA GCCTGGGCAG AGGTTTACAT AATTGCTGTA 
GAAAGACAGA AAAAACAAAC TGAGAAACAG AAGCAGACTA TGAAAATTGT TGGCTCTATA 
GAGGAAAATG CCAGAGATGT GTCATCTTCC ATAGATGGAC TTCTTGACTT AGTCCAAACA 
GATCTTGGAA CATTAAGCAA ACTCTGGCTT GCTGCTCTTC AAGATTTTGC TCTTCTAACT 
TTGCCTTCAG AATTTGCTTC CCAACTTCCT CTTGAAGGTG GAGCTTTCTA CACTGCAGAA 
ACTAGTGAAA ATGCAAAGCC ACACTATCAT AATTCGTGGG CTCTTATTCT TCATGCTACA 
GCATTGTGGC TCACTAGCAC AGGCTTCATT GTAGCTGACC CAGATGAAGG AGCAACTAAT 
CTCTCAAGAC CTGTAACACC GACTACAATG TGTCAGGGTT CTTCTGGGGC ATCAGTGAAA 
TCCCCTGAGG ATGTTTATAC AGACAGATTT CACCTTATCT TGGGAATCAG TGTGGAATTT 
CTCTGTTCCC TGCGTTCAGA TGCAACCATG GAAAGCATCA CTGCTTGTTT ACATGCACTG 
CAGGCTTTAC TGGATGTTCC TTGGCCCAGA TCTAAGATTG GCAGTGACCA GGACTTGGGA 
GTTGAGTTGT TGAATGTACT CCATCGAGTA ATACTGACTA GAGAGTCACC TTCCATTCAG 
TTAGCTTCAC TTGAAGTTGT AAGACAGGTT ATCTACGCAG CCCAAGAACA TGTAAAGGAA 
AAGAGGAGAA GTGCAGAAGT TGATGATGGA GCTGCTGAAA AGGAAACCCT GCCAGAATTT 
GGAGAAGGAA AGGATACGGG AGGACTGGTA CCTGGAAGAT CTCTGGTGTT TGCAACATTG 
GAGCTATGTG TATGCATTTT GGTCCGGCAA CTTCCAGAGT TAAATCCTAA ACTGACCGGT 
AGTCCAGGAA TAAAAACTAC AAAAGCACAG ATTCTTTCAG ATGATGGAAA TAAGTTGGTT 
TCAGCAGCAT TAACTATCCT CTCAGAACTT CCTGGAGTAT GCTCTCCCGA AGGAAGCATT 
TCAGTTCTCC CTACTATTCT GTACCTTATA ATTGGGGTCC TCCGAGAAAC TGCTGTGAGG 
TTACCTGGAG GACAGTTATC TTATACTGTT GGAGCTGCTC TACAAGCCTT GAAAGGAATA 
TTGGCATCTC CCTTGGCTCG AGCAGAGAAG AGTCACACTG CTTGGACTGA CCTTCTTCGA 
AGTGCTTTGA CAACAATCCT AGACTGCTGG GATCCAGATG GAACACAGCA AGAGTTTGAT 
GAAATCAGCC TGCTTACTGC CATAACAGTA TTTATCTTGT CTTCAAGTCC AGAAGTGACC 
ACCATCCAGT GCCTGCAAGA ACGTTGTATA CAGAAGTTTA AAGCTACTCT TGAGTTAAAA 
GACCCAGTAG TACAAATAAA GTGCTATCAG CTACTGCTTT CCATTTTCCA GTATCCAAAC 
CCAGCAGTAT CCTACCCTTA TATTCATTCA TTAGCATCAT CTATTGTGAG AAAACTGCAA 
GAGACAGATA AAGTAAAACC AGAAGATGCT GCTGAGCTTC AGGTGATTCA AGAAGGTATT 
AAGGTCTTAG AAGCCCTAGT AGGGATTGCG GAAGAACAGC ACCGTGCTCA CTTGGTGGCC 
GACATTCTCC CTATTCTTAT TTCCTTTCTT TTGGATGAAA ACACTCTGGG CTCAGCACCC 
ACCACAATGA GAAATTTACA TGACTTTGCT TTGCAGAATC TGATGCAGAT TGGTCCCCAG 
TATTCATCAA CTTTTAAAAA TATTATGGCT TCTTCTCCCA CAATGAAAGC CCGTCTTGAG 
GCAGCTATAA AAGGCAATCA GGAAAATATC AAAATCAAGA CAACCCCAAA ACATATCACA 
AAACCTGGAA AAAATTCAAG TATTCAGTTG AAGACCAATT TTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031808116.1
CDS	284..6451
Translation	MELAHSLLLNEEAYNQLGEVQKAEFVFEWLKFLEKLLLATNKADIKEKQKSIIEQLISLLNSSPGPPTRKLLAENLAVLYSIGDTFSVYQTIDKCNELIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRLLGNTFIDTVGNILKAMKSAESQGRYEIMLSLQNILSGLGAAAAPCHRDIYKAARSCLTDRSMAVRCAAAKCLLELQNEASFMWSTDIDSVATLCFKSFEGSNYDVRIAVSKLLGTVLARAVMSKPGTATSRQNVRKVSLEEAMELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFISTLGGRWLERNFSILLSHILSLVSQFHPKAIQTQMDAICCRRCVSFILRTTIGSLLGEKAQIAAARDICQVIWKLKKTVDAVMSEVNLETRPGSTDVAASQHVLVCALQELGNLIYGLGTTAAPLLQDSSAGILDNVISVIVHPSISVRLAAAWCLHCIAVALPSYLTPLLDSCLERLTVLKSSPEAVTGFSFAVAALLGAVKYCPLGIPHGKGKIIVMLAEDLLCSAAQNSRLSVQRTQAGWLLIAALMTLGSAFIHHHLPRVLRLWKCVFPLSPKDLETERSRGDSFTWQVTLEGRAGAFCAIKSFVSHCNDLLTDEVIQRLLPPLPCAVNLLTQLSSIQKTYGSPLKTPSVVYKQRLYELLALLPPEIYAGSLCAILKELAADLTSSDTQMAASTFLLPPLCHQDDLLLLSPLLQDTDHRFIEEQLLLGNGVACGSLEYDPFSVYEHGIEGDSVPKPLPPMLSLISSAARLFGIVCAHVAESQRLLVLEQLLDKIKHTKASRQQVVQLHVMAAFSNFLKYVANSKGNLGSEEIRRPALTLLMGALESTNPLMRCTAAEGLARLAQVIGDGSFTAGLAQVSFEKLKSARDVVTRTGHSLALGSLHRYLGGVGSSQHLNACIGILYTLSQDGTSPDVQTWALHSLSLIIDSAGPLYHLHVESTLSLILMLLLTVPPTHAEVHQSLGRCLNVLITTLGPELQGSSTAVSTLRTSCLLGCAIMQDNPDCLVQAQAISCLQQLHMFAPRYVNLSSLVSCLCEILLDNSVLVNLCSSYLFLRRAVLACLRQIVQREAAEVSEHAVVLAKDSREDLIPDANIREVGLEGALLNLLDKESDQRLCQDIKETLSHMITSMAVGKLSFWLKLCKDVLASSADFTTVASVDTTQDDEIDKEDDASVLTYRNDEKCRPITNPRWATRVFAAECVCKIISQCENSDSAHFDIALAQEMKERDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLQTLLVVVRQFATIPEPEFPGHVILEQYQANVGAALRPAFTSDTPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSQLYNESASTMEILAVLKAWAEVYIIAVERQKKQTEKQKQTMKIVGSIEENARDVSSSIDGLLDLVQTDLGTLSKLWLAALQDFALLTLPSEFASQLPLEGGAFYTAETSENAKPHYHNSWALILHATALWLTSTGFIVADPDEGATNLSRPVTPTTMCQGSSGASVKSPEDVYTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGVELLNVLHRVILTRESPSIQLASLEVVRQVIYAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGRSLVFATLELCVCILVRQLPELNPKLTGSPGIKTTKAQILSDDGNKLVSAALTILSELPGVCSPEGSISVLPTILYLIIGVLRETAVRLPGGQLSYTVGAALQALKGILASPLARAEKSHTAWTDLLRSALTTILDCWDPDGTQQEFDEISLLTAITVFILSSSPEVTTIQCLQERCIQKFKATLELKDPVVQIKCYQLLLSIFQYPNPAVSYPYIHSLASSIVRKLQETDKVKPEDAAELQVIQEGIKVLEALVGIAEEQHRAHLVADILPILISFLLDENTLGSAPTTMRNLHDFALQNLMQIGPQYSSTFKNIMASSPTMKARLEAAIKGNQENIKIKTTPKHITKPGKNSSIQLKTNFF

Target ORF information:

RefSeq Version	XM_031952256.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031952256.1

ORF Insert Sequence:

ATGGAACTGG CTCACAGCTT ATTGCTGAAT GAAGAAGCAT ATAATCAACT TGGAGAAGTT 
CAGAAGGCAG AATTTGTTTT TGAATGGTTG AAATTTTTAG AGAAACTTTT GCTAGCAACC 
AACAAGGCTG ACATAAAGGA GAAACAGAAG AGTATTATTG AGCAGCTCAT ATCTCTATTA 
AATAGCTCTC CAGGGCCACC TACCCGAAAG CTTCTTGCTG AGAACCTAGC TGTTCTTTAT 
AGTATTGGAG ATACATTTTC TGTTTATCAA ACAATTGACA AATGTAATGA ACTCATTCGT 
AGCAAAGATG ACTCTCCAAG TTATCTTCCC ACAAAACTTG CTGCTGTGGT ATGTCTGGGT 
TCTTTGTATA AGAAGTTGGG GAGACTATTG GGTAACACCT TTATAGATAC AGTGGGGAAT 
ATACTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGTCGAT ATGAGATTAT GCTGAGTTTG 
CAAAATATAC TGAGTGGACT TGGAGCTGCT GCTGCCCCTT GTCACAGAGA CATTTATAAA 
GCTGCAAGGT CATGTTTGAC AGACAGGTCC ATGGCAGTTC GCTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAGAATGA AGCTAGCTTT ATGTGGAGTA CAGACATAGA CAGTGTTGCA 
ACTTTGTGTT TTAAGTCATT TGAAGGTTCC AACTATGATG TGCGAATAGC AGTATCAAAG 
CTCTTAGGTA CAGTGTTGGC TAGAGCTGTA ATGTCCAAAC CTGGAACAGC AACTTCCCGG 
CAAAATGTTC GCAAAGTTTC TCTGGAGGAA GCCATGGAGT TACTAGGAAC AGGGTTTCTA 
CGTGGGAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGT TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG TGTTACTCAG GCATATGTGG TGTTTATTTC AACACTAGGA 
GGACGTTGGT TAGAAAGAAA TTTTTCCATC TTACTCTCTC ATATCCTTAG CCTAGTATCA 
CAGTTTCACC CTAAAGCAAT CCAAACTCAG ATGGATGCTA TCTGTTGTCG TCGCTGTGTT 
TCATTTATCC TTCGAACTAC TATAGGAAGC CTTCTCGGGG AAAAAGCTCA AATTGCTGCT 
GCCAGGGATA TTTGTCAGGT CATCTGGAAA CTAAAGAAAA CTGTTGATGC TGTAATGAGT 
GAAGTTAATT TGGAAACTCG ACCTGGCTCC ACAGATGTTG CTGCCAGCCA GCATGTGCTA 
GTATGTGCTT TACAAGAACT TGGAAATCTA ATATATGGTC TTGGCACAAC AGCTGCACCT 
TTATTACAGG ATTCAAGTGC AGGGATTCTT GACAATGTCA TTTCAGTTAT TGTTCATCCC 
AGCATTTCAG TTCGATTAGC AGCAGCTTGG TGTTTACACT GCATTGCTGT AGCATTGCCT 
TCCTACCTAA CTCCACTTTT GGACAGTTGC CTTGAACGAC TTACTGTGCT CAAATCCTCA 
CCTGAAGCTG TGACTGGCTT TAGTTTTGCT GTGGCAGCTC TACTAGGAGC AGTGAAATAT 
TGCCCTTTAG GAATTCCACA TGGAAAAGGA AAGATAATTG TGATGTTAGC AGAGGATTTG 
TTATGTTCTG CTGCTCAGAA TAGTCGCCTT TCAGTTCAAC GAACACAAGC TGGATGGTTG 
CTGATAGCTG CCTTAATGAC ATTAGGCTCT GCATTTATTC ATCACCACCT TCCCCGGGTG 
CTAAGATTAT GGAAATGTGT TTTTCCACTA TCTCCTAAGG ATCTAGAAAC AGAAAGAAGT 
CGAGGAGACT CATTTACATG GCAGGTGACC TTGGAAGGTC GTGCAGGGGC CTTCTGTGCT 
ATCAAGAGCT TTGTTTCCCA CTGTAATGAT CTTCTTACTG ATGAAGTCAT TCAGAGACTT 
CTTCCTCCCC TTCCGTGTGC TGTTAATTTA TTAACTCAAC TTTCTTCAAT ACAGAAAACA 
TATGGAAGTC CTTTAAAAAC TCCTTCAGTG GTTTACAAAC AAAGACTTTA TGAACTATTG 
GCTTTATTAC CTCCTGAGAT CTATGCAGGA AGCCTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACCTGA CTTCTTCTGA TACCCAGATG GCAGCATCCA CTTTCTTACT TCCACCCCTC 
TGTCACCAAG ATGATCTTTT ATTACTAAGT CCTTTGCTAC AAGACACTGA CCACAGATTT 
ATTGAAGAAC AGCTCCTCCT TGGTAATGGT GTTGCTTGTG GAAGTCTAGA GTATGACCCA 
TTTTCAGTTT ATGAACATGG CATAGAAGGG GATTCAGTAC CTAAGCCCCT ACCTCCAATG 
TTGTCACTTA TCAGCTCTGC AGCCAGGCTT TTTGGAATTG TATGTGCCCA TGTAGCAGAA 
TCTCAAAGGC TCTTGGTATT GGAACAACTT TTGGACAAAA TAAAACACAC AAAAGCATCT 
CGTCAACAAG TAGTACAATT ACATGTTATG GCAGCATTTT CTAATTTTTT GAAGTATGTG 
GCCAACTCCA AAGGAAATTT AGGCTCAGAA GAAATTAGAA GACCTGCACT AACATTATTG 
ATGGGAGCCT TGGAAAGTAC CAACCCCTTA ATGCGTTGTA CTGCTGCAGA AGGTTTGGCT 
AGATTAGCCC AAGTGATTGG TGATGGCTCC TTCACTGCTG GATTAGCACA GGTTAGCTTT 
GAAAAACTGA AATCAGCTAG GGATGTGGTA ACCAGAACAG GACATTCTTT GGCTCTGGGA 
TCCTTACATA GATATTTAGG AGGAGTGGGC TCTTCTCAGC ACTTAAATGC TTGCATCGGA 
ATACTTTACA CTCTGTCACA GGATGGAACT TCTCCTGATG TACAGACATG GGCCCTACAT 
TCTCTCTCAT TGATAATTGA TTCTGCTGGT CCACTGTACC ATTTACATGT GGAATCTACC 
CTCTCTCTTA TACTAATGTT GTTGTTGACT GTTCCACCTA CTCATGCTGA AGTTCACCAA 
AGTCTTGGAC GATGTTTGAA TGTACTCATC ACCACATTGG GTCCAGAACT ACAAGGTAGC 
AGTACTGCAG TTTCTACTTT ACGGACATCC TGTCTTTTGG GGTGTGCAAT AATGCAGGAT 
AACCCAGATT GCCTTGTTCA AGCTCAAGCC ATCTCTTGTC TTCAACAACT TCATATGTTT 
GCCCCACGCT ATGTCAATTT ATCTAGTCTA GTCAGCTGTC TTTGTGAGAT CTTGTTGGAT 
AATTCTGTGT TGGTGAATCT CTGTAGCTCC TACTTGTTTC TGAGAAGAGC AGTACTAGCT 
TGTTTACGTC AAATTGTGCA AAGGGAAGCA GCTGAGGTTT CAGAACATGC TGTTGTTCTT 
GCTAAGGACA GCAGAGAAGA CTTAATTCCA GATGCTAATA TCCGAGAAGT GGGTCTTGAG 
GGGGCATTGT TGAACTTACT AGACAAAGAA TCTGATCAGA GATTGTGCCA GGATATCAAA 
GAGACCCTGA GTCATATGAT CACATCAATG GCAGTGGGAA AGCTTTCTTT TTGGCTAAAA 
CTTTGTAAAG ATGTCCTTGC TTCATCAGCT GATTTTACAA CTGTTGCTTC TGTGGATACA 
ACTCAGGATG ATGAAATAGA TAAAGAAGAT GATGCTTCAG TCCTAACCTA CAGAAATGAT 
GAGAAATGCC GGCCAATTAC AAATCCACGA TGGGCTACTA GAGTTTTTGC TGCTGAATGT 
GTGTGTAAAA TAATCAGCCA GTGTGAGAAT TCAGACAGTG CTCACTTTGA CATTGCTTTG 
GCTCAAGAAA TGAAAGAAAG GGATTCACGA AATGATTTTT TGGTATTACA TCTTGCTGAC 
TTGATCCGCA TGGCTTTTAT GGCTGCAACA GACCACAGTG ACCAGCTCCG TCTTTCTGGT 
CTTCAGACAC TGTTAGTAGT TGTTCGGCAA TTTGCAACTA TTCCTGAACC AGAATTCCCA 
GGTCATGTGA TTCTGGAACA GTATCAAGCC AATGTGGGAG CAGCACTTAG ACCAGCTTTT 
ACTTCAGATA CACCACCAGA TGTAACTGCC AAAGCATGTC AGGTTTGCAG TGCCTGGATA 
GCAAGTGGAG TTGTAAGTGA TCTTAATGAT CTCCGAAGAG TTCATCAATT GCTTGTATCA 
TCCTTGACGA AAATACAGGC TGGAAAAGAA GCCTTGAGTC AATTATATAA CGAAAGTGCT 
TCTACTATGG AAATCCTAGC TGTTCTCAAA GCCTGGGCAG AGGTTTACAT AATTGCTGTA 
GAAAGACAGA AAAAACAAAC TGAGAAACAG AAGCAGACTA TGAAAATTGT TGGCTCTATA 
GAGGAAAATG CCAGAGATGT GTCATCTTCC ATAGATGGAC TTCTTGACTT AGTCCAAACA 
GATCTTGGAA CATTAAGCAA ACTCTGGCTT GCTGCTCTTC AAGATTTTGC TCTTCTAACT 
TTGCCTTCAG AATTTGCTTC CCAACTTCCT CTTGAAGGTG GAGCTTTCTA CACTGCAGAA 
ACTAGTGAAA ATGCAAAGCC ACACTATCAT AATTCGTGGG CTCTTATTCT TCATGCTACA 
GCATTGTGGC TCACTAGCAC AGGCTTCATT GTAGCTGACC CAGATGAAGG AGCAACTAAT 
CTCTCAAGAC CTGTAACACC GACTACAATG TGTCAGGGTT CTTCTGGGGC ATCAGTGAAA 
TCCCCTGAGG ATGTTTATAC AGACAGATTT CACCTTATCT TGGGAATCAG TGTGGAATTT 
CTCTGTTCCC TGCGTTCAGA TGCAACCATG GAAAGCATCA CTGCTTGTTT ACATGCACTG 
CAGGCTTTAC TGGATGTTCC TTGGCCCAGA TCTAAGATTG GCAGTGACCA GGACTTGGGA 
GTTGAGTTGT TGAATGTACT CCATCGAGTA ATACTGACTA GAGAGTCACC TTCCATTCAG 
TTAGCTTCAC TTGAAGTTGT AAGACAGGTT ATCTACGCAG CCCAAGAACA TGTAAAGGAA 
AAGAGGAGAA GTGCAGAAGT TGATGATGGA GCTGCTGAAA AGGAAACCCT GCCAGAATTT 
GGAGAAGGAA AGGATACGGG AGGACTGGTA CCTGGAAGAT CTCTGGTGTT TGCAACATTG 
GAGCTATGTG TATGCATTTT GGTCCGGCAA CTTCCAGAGT TAAATCCTAA ACTGACCGGT 
AGTCCAGGAA TAAAAACTAC AAAAGCACAG ATTCTTTCAG ATGATGGAAA TAAGTTGGTT 
TCAGCAGCAT TAACTATCCT CTCAGAACTT CCTGGAGTAT GCTCTCCCGA AGGAAGCATT 
TCAGTTCTCC CTACTATTCT GTACCTTATA ATTGGGGTCC TCCGAGAAAC TGCTGTGAGG 
TTACCTGGAG GACAGTTATC TTATACTGTT GGAGCTGCTC TACAAGCCTT GAAAGGAATA 
TTGGCATCTC CCTTGGCTCG AGCAGAGAAG AGTCACACTG CTTGGACTGA CCTTCTTCGA 
AGTGCTTTGA CAACAATCCT AGACTGCTGG GATCCAGATG GAACACAGCA AGAGTTTGAT 
GAAATCAGCC TGCTTACTGC CATAACAGTA TTTATCTTGT CTTCAAGTCC AGAAGTGACC 
ACCATCCAGT GCCTGCAAGA ACGTTGTATA CAGAAGTTTA AAGCTACTCT TGAGTTAAAA 
GACCCAGTAG TACAAATAAA GTGCTATCAG CTACTGCTTT CCATTTTCCA GTATCCAAAC 
CCAGCAGTAT CCTACCCTTA TATTCATTCA TTAGCATCAT CTATTGTGAG AAAACTGCAA 
GAGACAGATA AAGTAAAACC AGAAGATGCT GCTGAGCTTC AGGTGATTCA AGAAGGTATT 
AAGGTCTTAG AAGCCCTAGT AGGGATTGCG GAAGAACAGC ACCGTGCTCA CTTGGTGGCC 
GACATTCTCC CTATTCTTAT TTCCTTTCTT TTGGATGAAA ACACTCTGGG CTCAGCACCC 
ACCACAATGA GAAATTTACA TGACTTTGCT TTGCAGAATC TGATGCAGAT TGGTCCCCAG 
TATTCATCAA CTTTTAAAAA TATTATGGCT TCTTCTCCCA CAATGAAAGC CCGTCTTGAG 
GCAGCTATAA AAGGCAATCA GGAAAATATC AAAATCAAGA CAACCCCAAA ACATATCACA 
AAACCTGGAA AAAATTCAAG TATTCAGTTG AAGACCAATT TTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_031952259.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031808119.1 Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X3, mRNA.

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CloneID	OSa225025
Clone ID Related Accession (Same CDS sequence)	XM_031952257.1 , XM_031952256.1 , XM_031952259.1 , XM_031952260.1
Accession Version	XM_031952259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG CTCACAGCTT ATTGCTGAAT GAAGAAGCAT ATAATCAACT TGGAGAAGTT 
CAGAAGGCAG AATTTGTTTT TGAATGGTTG AAATTTTTAG AGAAACTTTT GCTAGCAACC 
AACAAGGCTG ACATAAAGGA GAAACAGAAG AGTATTATTG AGCAGCTCAT ATCTCTATTA 
AATAGCTCTC CAGGGCCACC TACCCGAAAG CTTCTTGCTG AGAACCTAGC TGTTCTTTAT 
AGTATTGGAG ATACATTTTC TGTTTATCAA ACAATTGACA AATGTAATGA ACTCATTCGT 
AGCAAAGATG ACTCTCCAAG TTATCTTCCC ACAAAACTTG CTGCTGTGGT ATGTCTGGGT 
TCTTTGTATA AGAAGTTGGG GAGACTATTG GGTAACACCT TTATAGATAC AGTGGGGAAT 
ATACTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGTCGAT ATGAGATTAT GCTGAGTTTG 
CAAAATATAC TGAGTGGACT TGGAGCTGCT GCTGCCCCTT GTCACAGAGA CATTTATAAA 
GCTGCAAGGT CATGTTTGAC AGACAGGTCC ATGGCAGTTC GCTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAGAATGA AGCTAGCTTT ATGTGGAGTA CAGACATAGA CAGTGTTGCA 
ACTTTGTGTT TTAAGTCATT TGAAGGTTCC AACTATGATG TGCGAATAGC AGTATCAAAG 
CTCTTAGGTA CAGTGTTGGC TAGAGCTGTA ATGTCCAAAC CTGGAACAGC AACTTCCCGG 
CAAAATGTTC GCAAAGTTTC TCTGGAGGAA GCCATGGAGT TACTAGGAAC AGGGTTTCTA 
CGTGGGAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGT TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG TGTTACTCAG GCATATGTGG TGTTTATTTC AACACTAGGA 
GGACGTTGGT TAGAAAGAAA TTTTTCCATC TTACTCTCTC ATATCCTTAG CCTAGTATCA 
CAGTTTCACC CTAAAGCAAT CCAAACTCAG ATGGATGCTA TCTGTTGTCG TCGCTGTGTT 
TCATTTATCC TTCGAACTAC TATAGGAAGC CTTCTCGGGG AAAAAGCTCA AATTGCTGCT 
GCCAGGGATA TTTGTCAGGT CATCTGGAAA CTAAAGAAAA CTGTTGATGC TGTAATGAGT 
GAAGTTAATT TGGAAACTCG ACCTGGCTCC ACAGATGTTG CTGCCAGCCA GCATGTGCTA 
GTATGTGCTT TACAAGAACT TGGAAATCTA ATATATGGTC TTGGCACAAC AGCTGCACCT 
TTATTACAGG ATTCAAGTGC AGGGATTCTT GACAATGTCA TTTCAGTTAT TGTTCATCCC 
AGCATTTCAG TTCGATTAGC AGCAGCTTGG TGTTTACACT GCATTGCTGT AGCATTGCCT 
TCCTACCTAA CTCCACTTTT GGACAGTTGC CTTGAACGAC TTACTGTGCT CAAATCCTCA 
CCTGAAGCTG TGACTGGCTT TAGTTTTGCT GTGGCAGCTC TACTAGGAGC AGTGAAATAT 
TGCCCTTTAG GAATTCCACA TGGAAAAGGA AAGATAATTG TGATGTTAGC AGAGGATTTG 
TTATGTTCTG CTGCTCAGAA TAGTCGCCTT TCAGTTCAAC GAACACAAGC TGGATGGTTG 
CTGATAGCTG CCTTAATGAC ATTAGGCTCT GCATTTATTC ATCACCACCT TCCCCGGGTG 
CTAAGATTAT GGAAATGTGT TTTTCCACTA TCTCCTAAGG ATCTAGAAAC AGAAAGAAGT 
CGAGGAGACT CATTTACATG GCAGGTGACC TTGGAAGGTC GTGCAGGGGC CTTCTGTGCT 
ATCAAGAGCT TTGTTTCCCA CTGTAATGAT CTTCTTACTG ATGAAGTCAT TCAGAGACTT 
CTTCCTCCCC TTCCGTGTGC TGTTAATTTA TTAACTCAAC TTTCTTCAAT ACAGAAAACA 
TATGGAAGTC CTTTAAAAAC TCCTTCAGTG GTTTACAAAC AAAGACTTTA TGAACTATTG 
GCTTTATTAC CTCCTGAGAT CTATGCAGGA AGCCTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACCTGA CTTCTTCTGA TACCCAGATG GCAGCATCCA CTTTCTTACT TCCACCCCTC 
TGTCACCAAG ATGATCTTTT ATTACTAAGT CCTTTGCTAC AAGACACTGA CCACAGATTT 
ATTGAAGAAC AGCTCCTCCT TGGTAATGGT GTTGCTTGTG GAAGTCTAGA GTATGACCCA 
TTTTCAGTTT ATGAACATGG CATAGAAGGG GATTCAGTAC CTAAGCCCCT ACCTCCAATG 
TTGTCACTTA TCAGCTCTGC AGCCAGGCTT TTTGGAATTG TATGTGCCCA TGTAGCAGAA 
TCTCAAAGGC TCTTGGTATT GGAACAACTT TTGGACAAAA TAAAACACAC AAAAGCATCT 
CGTCAACAAG TAGTACAATT ACATGTTATG GCAGCATTTT CTAATTTTTT GAAGTATGTG 
GCCAACTCCA AAGGAAATTT AGGCTCAGAA GAAATTAGAA GACCTGCACT AACATTATTG 
ATGGGAGCCT TGGAAAGTAC CAACCCCTTA ATGCGTTGTA CTGCTGCAGA AGGTTTGGCT 
AGATTAGCCC AAGTGATTGG TGATGGCTCC TTCACTGCTG GATTAGCACA GGTTAGCTTT 
GAAAAACTGA AATCAGCTAG GGATGTGGTA ACCAGAACAG GACATTCTTT GGCTCTGGGA 
TCCTTACATA GATATTTAGG AGGAGTGGGC TCTTCTCAGC ACTTAAATGC TTGCATCGGA 
ATACTTTACA CTCTGTCACA GGATGGAACT TCTCCTGATG TACAGACATG GGCCCTACAT 
TCTCTCTCAT TGATAATTGA TTCTGCTGGT CCACTGTACC ATTTACATGT GGAATCTACC 
CTCTCTCTTA TACTAATGTT GTTGTTGACT GTTCCACCTA CTCATGCTGA AGTTCACCAA 
AGTCTTGGAC GATGTTTGAA TGTACTCATC ACCACATTGG GTCCAGAACT ACAAGGTAGC 
AGTACTGCAG TTTCTACTTT ACGGACATCC TGTCTTTTGG GGTGTGCAAT AATGCAGGAT 
AACCCAGATT GCCTTGTTCA AGCTCAAGCC ATCTCTTGTC TTCAACAACT TCATATGTTT 
GCCCCACGCT ATGTCAATTT ATCTAGTCTA GTCAGCTGTC TTTGTGAGAT CTTGTTGGAT 
AATTCTGTGT TGGTGAATCT CTGTAGCTCC TACTTGTTTC TGAGAAGAGC AGTACTAGCT 
TGTTTACGTC AAATTGTGCA AAGGGAAGCA GCTGAGGTTT CAGAACATGC TGTTGTTCTT 
GCTAAGGACA GCAGAGAAGA CTTAATTCCA GATGCTAATA TCCGAGAAGT GGGTCTTGAG 
GGGGCATTGT TGAACTTACT AGACAAAGAA TCTGATCAGA GATTGTGCCA GGATATCAAA 
GAGACCCTGA GTCATATGAT CACATCAATG GCAGTGGGAA AGCTTTCTTT TTGGCTAAAA 
CTTTGTAAAG ATGTCCTTGC TTCATCAGCT GATTTTACAA CTGTTGCTTC TGTGGATACA 
ACTCAGGATG ATGAAATAGA TAAAGAAGAT GATGCTTCAG TCCTAACCTA CAGAAATGAT 
GAGAAATGCC GGCCAATTAC AAATCCACGA TGGGCTACTA GAGTTTTTGC TGCTGAATGT 
GTGTGTAAAA TAATCAGCCA GTGTGAGAAT TCAGACAGTG CTCACTTTGA CATTGCTTTG 
GCTCAAGAAA TGAAAGAAAG GGATTCACGA AATGATTTTT TGGTATTACA TCTTGCTGAC 
TTGATCCGCA TGGCTTTTAT GGCTGCAACA GACCACAGTG ACCAGCTCCG TCTTTCTGGT 
CTTCAGACAC TGTTAGTAGT TGTTCGGCAA TTTGCAACTA TTCCTGAACC AGAATTCCCA 
GGTCATGTGA TTCTGGAACA GTATCAAGCC AATGTGGGAG CAGCACTTAG ACCAGCTTTT 
ACTTCAGATA CACCACCAGA TGTAACTGCC AAAGCATGTC AGGTTTGCAG TGCCTGGATA 
GCAAGTGGAG TTGTAAGTGA TCTTAATGAT CTCCGAAGAG TTCATCAATT GCTTGTATCA 
TCCTTGACGA AAATACAGGC TGGAAAAGAA GCCTTGAGTC AATTATATAA CGAAAGTGCT 
TCTACTATGG AAATCCTAGC TGTTCTCAAA GCCTGGGCAG AGGTTTACAT AATTGCTGTA 
GAAAGACAGA AAAAACAAAC TGAGAAACAG AAGCAGACTA TGAAAATTGT TGGCTCTATA 
GAGGAAAATG CCAGAGATGT GTCATCTTCC ATAGATGGAC TTCTTGACTT AGTCCAAACA 
GATCTTGGAA CATTAAGCAA ACTCTGGCTT GCTGCTCTTC AAGATTTTGC TCTTCTAACT 
TTGCCTTCAG AATTTGCTTC CCAACTTCCT CTTGAAGGTG GAGCTTTCTA CACTGCAGAA 
ACTAGTGAAA ATGCAAAGCC ACACTATCAT AATTCGTGGG CTCTTATTCT TCATGCTACA 
GCATTGTGGC TCACTAGCAC AGGCTTCATT GTAGCTGACC CAGATGAAGG AGCAACTAAT 
CTCTCAAGAC CTGTAACACC GACTACAATG TGTCAGGGTT CTTCTGGGGC ATCAGTGAAA 
TCCCCTGAGG ATGTTTATAC AGACAGATTT CACCTTATCT TGGGAATCAG TGTGGAATTT 
CTCTGTTCCC TGCGTTCAGA TGCAACCATG GAAAGCATCA CTGCTTGTTT ACATGCACTG 
CAGGCTTTAC TGGATGTTCC TTGGCCCAGA TCTAAGATTG GCAGTGACCA GGACTTGGGA 
GTTGAGTTGT TGAATGTACT CCATCGAGTA ATACTGACTA GAGAGTCACC TTCCATTCAG 
TTAGCTTCAC TTGAAGTTGT AAGACAGGTT ATCTACGCAG CCCAAGAACA TGTAAAGGAA 
AAGAGGAGAA GTGCAGAAGT TGATGATGGA GCTGCTGAAA AGGAAACCCT GCCAGAATTT 
GGAGAAGGAA AGGATACGGG AGGACTGGTA CCTGGAAGAT CTCTGGTGTT TGCAACATTG 
GAGCTATGTG TATGCATTTT GGTCCGGCAA CTTCCAGAGT TAAATCCTAA ACTGACCGGT 
AGTCCAGGAA TAAAAACTAC AAAAGCACAG ATTCTTTCAG ATGATGGAAA TAAGTTGGTT 
TCAGCAGCAT TAACTATCCT CTCAGAACTT CCTGGAGTAT GCTCTCCCGA AGGAAGCATT 
TCAGTTCTCC CTACTATTCT GTACCTTATA ATTGGGGTCC TCCGAGAAAC TGCTGTGAGG 
TTACCTGGAG GACAGTTATC TTATACTGTT GGAGCTGCTC TACAAGCCTT GAAAGGAATA 
TTGGCATCTC CCTTGGCTCG AGCAGAGAAG AGTCACACTG CTTGGACTGA CCTTCTTCGA 
AGTGCTTTGA CAACAATCCT AGACTGCTGG GATCCAGATG GAACACAGCA AGAGTTTGAT 
GAAATCAGCC TGCTTACTGC CATAACAGTA TTTATCTTGT CTTCAAGTCC AGAAGTGACC 
ACCATCCAGT GCCTGCAAGA ACGTTGTATA CAGAAGTTTA AAGCTACTCT TGAGTTAAAA 
GACCCAGTAG TACAAATAAA GTGCTATCAG CTACTGCTTT CCATTTTCCA GTATCCAAAC 
CCAGCAGTAT CCTACCCTTA TATTCATTCA TTAGCATCAT CTATTGTGAG AAAACTGCAA 
GAGACAGATA AAGTAAAACC AGAAGATGCT GCTGAGCTTC AGGTGATTCA AGAAGGTATT 
AAGGTCTTAG AAGCCCTAGT AGGGATTGCG GAAGAACAGC ACCGTGCTCA CTTGGTGGCC 
GACATTCTCC CTATTCTTAT TTCCTTTCTT TTGGATGAAA ACACTCTGGG CTCAGCACCC 
ACCACAATGA GAAATTTACA TGACTTTGCT TTGCAGAATC TGATGCAGAT TGGTCCCCAG 
TATTCATCAA CTTTTAAAAA TATTATGGCT TCTTCTCCCA CAATGAAAGC CCGTCTTGAG 
GCAGCTATAA AAGGCAATCA GGAAAATATC AAAATCAAGA CAACCCCAAA ACATATCACA 
AAACCTGGAA AAAATTCAAG TATTCAGTTG AAGACCAATT TTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031808119.1
CDS	1953..8120
Translation	MELAHSLLLNEEAYNQLGEVQKAEFVFEWLKFLEKLLLATNKADIKEKQKSIIEQLISLLNSSPGPPTRKLLAENLAVLYSIGDTFSVYQTIDKCNELIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRLLGNTFIDTVGNILKAMKSAESQGRYEIMLSLQNILSGLGAAAAPCHRDIYKAARSCLTDRSMAVRCAAAKCLLELQNEASFMWSTDIDSVATLCFKSFEGSNYDVRIAVSKLLGTVLARAVMSKPGTATSRQNVRKVSLEEAMELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFISTLGGRWLERNFSILLSHILSLVSQFHPKAIQTQMDAICCRRCVSFILRTTIGSLLGEKAQIAAARDICQVIWKLKKTVDAVMSEVNLETRPGSTDVAASQHVLVCALQELGNLIYGLGTTAAPLLQDSSAGILDNVISVIVHPSISVRLAAAWCLHCIAVALPSYLTPLLDSCLERLTVLKSSPEAVTGFSFAVAALLGAVKYCPLGIPHGKGKIIVMLAEDLLCSAAQNSRLSVQRTQAGWLLIAALMTLGSAFIHHHLPRVLRLWKCVFPLSPKDLETERSRGDSFTWQVTLEGRAGAFCAIKSFVSHCNDLLTDEVIQRLLPPLPCAVNLLTQLSSIQKTYGSPLKTPSVVYKQRLYELLALLPPEIYAGSLCAILKELAADLTSSDTQMAASTFLLPPLCHQDDLLLLSPLLQDTDHRFIEEQLLLGNGVACGSLEYDPFSVYEHGIEGDSVPKPLPPMLSLISSAARLFGIVCAHVAESQRLLVLEQLLDKIKHTKASRQQVVQLHVMAAFSNFLKYVANSKGNLGSEEIRRPALTLLMGALESTNPLMRCTAAEGLARLAQVIGDGSFTAGLAQVSFEKLKSARDVVTRTGHSLALGSLHRYLGGVGSSQHLNACIGILYTLSQDGTSPDVQTWALHSLSLIIDSAGPLYHLHVESTLSLILMLLLTVPPTHAEVHQSLGRCLNVLITTLGPELQGSSTAVSTLRTSCLLGCAIMQDNPDCLVQAQAISCLQQLHMFAPRYVNLSSLVSCLCEILLDNSVLVNLCSSYLFLRRAVLACLRQIVQREAAEVSEHAVVLAKDSREDLIPDANIREVGLEGALLNLLDKESDQRLCQDIKETLSHMITSMAVGKLSFWLKLCKDVLASSADFTTVASVDTTQDDEIDKEDDASVLTYRNDEKCRPITNPRWATRVFAAECVCKIISQCENSDSAHFDIALAQEMKERDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLQTLLVVVRQFATIPEPEFPGHVILEQYQANVGAALRPAFTSDTPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSQLYNESASTMEILAVLKAWAEVYIIAVERQKKQTEKQKQTMKIVGSIEENARDVSSSIDGLLDLVQTDLGTLSKLWLAALQDFALLTLPSEFASQLPLEGGAFYTAETSENAKPHYHNSWALILHATALWLTSTGFIVADPDEGATNLSRPVTPTTMCQGSSGASVKSPEDVYTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGVELLNVLHRVILTRESPSIQLASLEVVRQVIYAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGRSLVFATLELCVCILVRQLPELNPKLTGSPGIKTTKAQILSDDGNKLVSAALTILSELPGVCSPEGSISVLPTILYLIIGVLRETAVRLPGGQLSYTVGAALQALKGILASPLARAEKSHTAWTDLLRSALTTILDCWDPDGTQQEFDEISLLTAITVFILSSSPEVTTIQCLQERCIQKFKATLELKDPVVQIKCYQLLLSIFQYPNPAVSYPYIHSLASSIVRKLQETDKVKPEDAAELQVIQEGIKVLEALVGIAEEQHRAHLVADILPILISFLLDENTLGSAPTTMRNLHDFALQNLMQIGPQYSSTFKNIMASSPTMKARLEAAIKGNQENIKIKTTPKHITKPGKNSSIQLKTNFF

Target ORF information:

RefSeq Version	XM_031952259.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031952259.1

ORF Insert Sequence:

ATGGAACTGG CTCACAGCTT ATTGCTGAAT GAAGAAGCAT ATAATCAACT TGGAGAAGTT 
CAGAAGGCAG AATTTGTTTT TGAATGGTTG AAATTTTTAG AGAAACTTTT GCTAGCAACC 
AACAAGGCTG ACATAAAGGA GAAACAGAAG AGTATTATTG AGCAGCTCAT ATCTCTATTA 
AATAGCTCTC CAGGGCCACC TACCCGAAAG CTTCTTGCTG AGAACCTAGC TGTTCTTTAT 
AGTATTGGAG ATACATTTTC TGTTTATCAA ACAATTGACA AATGTAATGA ACTCATTCGT 
AGCAAAGATG ACTCTCCAAG TTATCTTCCC ACAAAACTTG CTGCTGTGGT ATGTCTGGGT 
TCTTTGTATA AGAAGTTGGG GAGACTATTG GGTAACACCT TTATAGATAC AGTGGGGAAT 
ATACTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGTCGAT ATGAGATTAT GCTGAGTTTG 
CAAAATATAC TGAGTGGACT TGGAGCTGCT GCTGCCCCTT GTCACAGAGA CATTTATAAA 
GCTGCAAGGT CATGTTTGAC AGACAGGTCC ATGGCAGTTC GCTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAGAATGA AGCTAGCTTT ATGTGGAGTA CAGACATAGA CAGTGTTGCA 
ACTTTGTGTT TTAAGTCATT TGAAGGTTCC AACTATGATG TGCGAATAGC AGTATCAAAG 
CTCTTAGGTA CAGTGTTGGC TAGAGCTGTA ATGTCCAAAC CTGGAACAGC AACTTCCCGG 
CAAAATGTTC GCAAAGTTTC TCTGGAGGAA GCCATGGAGT TACTAGGAAC AGGGTTTCTA 
CGTGGGAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGT TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG TGTTACTCAG GCATATGTGG TGTTTATTTC AACACTAGGA 
GGACGTTGGT TAGAAAGAAA TTTTTCCATC TTACTCTCTC ATATCCTTAG CCTAGTATCA 
CAGTTTCACC CTAAAGCAAT CCAAACTCAG ATGGATGCTA TCTGTTGTCG TCGCTGTGTT 
TCATTTATCC TTCGAACTAC TATAGGAAGC CTTCTCGGGG AAAAAGCTCA AATTGCTGCT 
GCCAGGGATA TTTGTCAGGT CATCTGGAAA CTAAAGAAAA CTGTTGATGC TGTAATGAGT 
GAAGTTAATT TGGAAACTCG ACCTGGCTCC ACAGATGTTG CTGCCAGCCA GCATGTGCTA 
GTATGTGCTT TACAAGAACT TGGAAATCTA ATATATGGTC TTGGCACAAC AGCTGCACCT 
TTATTACAGG ATTCAAGTGC AGGGATTCTT GACAATGTCA TTTCAGTTAT TGTTCATCCC 
AGCATTTCAG TTCGATTAGC AGCAGCTTGG TGTTTACACT GCATTGCTGT AGCATTGCCT 
TCCTACCTAA CTCCACTTTT GGACAGTTGC CTTGAACGAC TTACTGTGCT CAAATCCTCA 
CCTGAAGCTG TGACTGGCTT TAGTTTTGCT GTGGCAGCTC TACTAGGAGC AGTGAAATAT 
TGCCCTTTAG GAATTCCACA TGGAAAAGGA AAGATAATTG TGATGTTAGC AGAGGATTTG 
TTATGTTCTG CTGCTCAGAA TAGTCGCCTT TCAGTTCAAC GAACACAAGC TGGATGGTTG 
CTGATAGCTG CCTTAATGAC ATTAGGCTCT GCATTTATTC ATCACCACCT TCCCCGGGTG 
CTAAGATTAT GGAAATGTGT TTTTCCACTA TCTCCTAAGG ATCTAGAAAC AGAAAGAAGT 
CGAGGAGACT CATTTACATG GCAGGTGACC TTGGAAGGTC GTGCAGGGGC CTTCTGTGCT 
ATCAAGAGCT TTGTTTCCCA CTGTAATGAT CTTCTTACTG ATGAAGTCAT TCAGAGACTT 
CTTCCTCCCC TTCCGTGTGC TGTTAATTTA TTAACTCAAC TTTCTTCAAT ACAGAAAACA 
TATGGAAGTC CTTTAAAAAC TCCTTCAGTG GTTTACAAAC AAAGACTTTA TGAACTATTG 
GCTTTATTAC CTCCTGAGAT CTATGCAGGA AGCCTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACCTGA CTTCTTCTGA TACCCAGATG GCAGCATCCA CTTTCTTACT TCCACCCCTC 
TGTCACCAAG ATGATCTTTT ATTACTAAGT CCTTTGCTAC AAGACACTGA CCACAGATTT 
ATTGAAGAAC AGCTCCTCCT TGGTAATGGT GTTGCTTGTG GAAGTCTAGA GTATGACCCA 
TTTTCAGTTT ATGAACATGG CATAGAAGGG GATTCAGTAC CTAAGCCCCT ACCTCCAATG 
TTGTCACTTA TCAGCTCTGC AGCCAGGCTT TTTGGAATTG TATGTGCCCA TGTAGCAGAA 
TCTCAAAGGC TCTTGGTATT GGAACAACTT TTGGACAAAA TAAAACACAC AAAAGCATCT 
CGTCAACAAG TAGTACAATT ACATGTTATG GCAGCATTTT CTAATTTTTT GAAGTATGTG 
GCCAACTCCA AAGGAAATTT AGGCTCAGAA GAAATTAGAA GACCTGCACT AACATTATTG 
ATGGGAGCCT TGGAAAGTAC CAACCCCTTA ATGCGTTGTA CTGCTGCAGA AGGTTTGGCT 
AGATTAGCCC AAGTGATTGG TGATGGCTCC TTCACTGCTG GATTAGCACA GGTTAGCTTT 
GAAAAACTGA AATCAGCTAG GGATGTGGTA ACCAGAACAG GACATTCTTT GGCTCTGGGA 
TCCTTACATA GATATTTAGG AGGAGTGGGC TCTTCTCAGC ACTTAAATGC TTGCATCGGA 
ATACTTTACA CTCTGTCACA GGATGGAACT TCTCCTGATG TACAGACATG GGCCCTACAT 
TCTCTCTCAT TGATAATTGA TTCTGCTGGT CCACTGTACC ATTTACATGT GGAATCTACC 
CTCTCTCTTA TACTAATGTT GTTGTTGACT GTTCCACCTA CTCATGCTGA AGTTCACCAA 
AGTCTTGGAC GATGTTTGAA TGTACTCATC ACCACATTGG GTCCAGAACT ACAAGGTAGC 
AGTACTGCAG TTTCTACTTT ACGGACATCC TGTCTTTTGG GGTGTGCAAT AATGCAGGAT 
AACCCAGATT GCCTTGTTCA AGCTCAAGCC ATCTCTTGTC TTCAACAACT TCATATGTTT 
GCCCCACGCT ATGTCAATTT ATCTAGTCTA GTCAGCTGTC TTTGTGAGAT CTTGTTGGAT 
AATTCTGTGT TGGTGAATCT CTGTAGCTCC TACTTGTTTC TGAGAAGAGC AGTACTAGCT 
TGTTTACGTC AAATTGTGCA AAGGGAAGCA GCTGAGGTTT CAGAACATGC TGTTGTTCTT 
GCTAAGGACA GCAGAGAAGA CTTAATTCCA GATGCTAATA TCCGAGAAGT GGGTCTTGAG 
GGGGCATTGT TGAACTTACT AGACAAAGAA TCTGATCAGA GATTGTGCCA GGATATCAAA 
GAGACCCTGA GTCATATGAT CACATCAATG GCAGTGGGAA AGCTTTCTTT TTGGCTAAAA 
CTTTGTAAAG ATGTCCTTGC TTCATCAGCT GATTTTACAA CTGTTGCTTC TGTGGATACA 
ACTCAGGATG ATGAAATAGA TAAAGAAGAT GATGCTTCAG TCCTAACCTA CAGAAATGAT 
GAGAAATGCC GGCCAATTAC AAATCCACGA TGGGCTACTA GAGTTTTTGC TGCTGAATGT 
GTGTGTAAAA TAATCAGCCA GTGTGAGAAT TCAGACAGTG CTCACTTTGA CATTGCTTTG 
GCTCAAGAAA TGAAAGAAAG GGATTCACGA AATGATTTTT TGGTATTACA TCTTGCTGAC 
TTGATCCGCA TGGCTTTTAT GGCTGCAACA GACCACAGTG ACCAGCTCCG TCTTTCTGGT 
CTTCAGACAC TGTTAGTAGT TGTTCGGCAA TTTGCAACTA TTCCTGAACC AGAATTCCCA 
GGTCATGTGA TTCTGGAACA GTATCAAGCC AATGTGGGAG CAGCACTTAG ACCAGCTTTT 
ACTTCAGATA CACCACCAGA TGTAACTGCC AAAGCATGTC AGGTTTGCAG TGCCTGGATA 
GCAAGTGGAG TTGTAAGTGA TCTTAATGAT CTCCGAAGAG TTCATCAATT GCTTGTATCA 
TCCTTGACGA AAATACAGGC TGGAAAAGAA GCCTTGAGTC AATTATATAA CGAAAGTGCT 
TCTACTATGG AAATCCTAGC TGTTCTCAAA GCCTGGGCAG AGGTTTACAT AATTGCTGTA 
GAAAGACAGA AAAAACAAAC TGAGAAACAG AAGCAGACTA TGAAAATTGT TGGCTCTATA 
GAGGAAAATG CCAGAGATGT GTCATCTTCC ATAGATGGAC TTCTTGACTT AGTCCAAACA 
GATCTTGGAA CATTAAGCAA ACTCTGGCTT GCTGCTCTTC AAGATTTTGC TCTTCTAACT 
TTGCCTTCAG AATTTGCTTC CCAACTTCCT CTTGAAGGTG GAGCTTTCTA CACTGCAGAA 
ACTAGTGAAA ATGCAAAGCC ACACTATCAT AATTCGTGGG CTCTTATTCT TCATGCTACA 
GCATTGTGGC TCACTAGCAC AGGCTTCATT GTAGCTGACC CAGATGAAGG AGCAACTAAT 
CTCTCAAGAC CTGTAACACC GACTACAATG TGTCAGGGTT CTTCTGGGGC ATCAGTGAAA 
TCCCCTGAGG ATGTTTATAC AGACAGATTT CACCTTATCT TGGGAATCAG TGTGGAATTT 
CTCTGTTCCC TGCGTTCAGA TGCAACCATG GAAAGCATCA CTGCTTGTTT ACATGCACTG 
CAGGCTTTAC TGGATGTTCC TTGGCCCAGA TCTAAGATTG GCAGTGACCA GGACTTGGGA 
GTTGAGTTGT TGAATGTACT CCATCGAGTA ATACTGACTA GAGAGTCACC TTCCATTCAG 
TTAGCTTCAC TTGAAGTTGT AAGACAGGTT ATCTACGCAG CCCAAGAACA TGTAAAGGAA 
AAGAGGAGAA GTGCAGAAGT TGATGATGGA GCTGCTGAAA AGGAAACCCT GCCAGAATTT 
GGAGAAGGAA AGGATACGGG AGGACTGGTA CCTGGAAGAT CTCTGGTGTT TGCAACATTG 
GAGCTATGTG TATGCATTTT GGTCCGGCAA CTTCCAGAGT TAAATCCTAA ACTGACCGGT 
AGTCCAGGAA TAAAAACTAC AAAAGCACAG ATTCTTTCAG ATGATGGAAA TAAGTTGGTT 
TCAGCAGCAT TAACTATCCT CTCAGAACTT CCTGGAGTAT GCTCTCCCGA AGGAAGCATT 
TCAGTTCTCC CTACTATTCT GTACCTTATA ATTGGGGTCC TCCGAGAAAC TGCTGTGAGG 
TTACCTGGAG GACAGTTATC TTATACTGTT GGAGCTGCTC TACAAGCCTT GAAAGGAATA 
TTGGCATCTC CCTTGGCTCG AGCAGAGAAG AGTCACACTG CTTGGACTGA CCTTCTTCGA 
AGTGCTTTGA CAACAATCCT AGACTGCTGG GATCCAGATG GAACACAGCA AGAGTTTGAT 
GAAATCAGCC TGCTTACTGC CATAACAGTA TTTATCTTGT CTTCAAGTCC AGAAGTGACC 
ACCATCCAGT GCCTGCAAGA ACGTTGTATA CAGAAGTTTA AAGCTACTCT TGAGTTAAAA 
GACCCAGTAG TACAAATAAA GTGCTATCAG CTACTGCTTT CCATTTTCCA GTATCCAAAC 
CCAGCAGTAT CCTACCCTTA TATTCATTCA TTAGCATCAT CTATTGTGAG AAAACTGCAA 
GAGACAGATA AAGTAAAACC AGAAGATGCT GCTGAGCTTC AGGTGATTCA AGAAGGTATT 
AAGGTCTTAG AAGCCCTAGT AGGGATTGCG GAAGAACAGC ACCGTGCTCA CTTGGTGGCC 
GACATTCTCC CTATTCTTAT TTCCTTTCTT TTGGATGAAA ACACTCTGGG CTCAGCACCC 
ACCACAATGA GAAATTTACA TGACTTTGCT TTGCAGAATC TGATGCAGAT TGGTCCCCAG 
TATTCATCAA CTTTTAAAAA TATTATGGCT TCTTCTCCCA CAATGAAAGC CCGTCTTGAG 
GCAGCTATAA AAGGCAATCA GGAAAATATC AAAATCAAGA CAACCCCAAA ACATATCACA 
AAACCTGGAA AAAATTCAAG TATTCAGTTG AAGACCAATT TTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_031952260.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031808120.1 Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X4, mRNA.

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CloneID	OSa225025
Clone ID Related Accession (Same CDS sequence)	XM_031952257.1 , XM_031952256.1 , XM_031952259.1 , XM_031952260.1
Accession Version	XM_031952260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG CTCACAGCTT ATTGCTGAAT GAAGAAGCAT ATAATCAACT TGGAGAAGTT 
CAGAAGGCAG AATTTGTTTT TGAATGGTTG AAATTTTTAG AGAAACTTTT GCTAGCAACC 
AACAAGGCTG ACATAAAGGA GAAACAGAAG AGTATTATTG AGCAGCTCAT ATCTCTATTA 
AATAGCTCTC CAGGGCCACC TACCCGAAAG CTTCTTGCTG AGAACCTAGC TGTTCTTTAT 
AGTATTGGAG ATACATTTTC TGTTTATCAA ACAATTGACA AATGTAATGA ACTCATTCGT 
AGCAAAGATG ACTCTCCAAG TTATCTTCCC ACAAAACTTG CTGCTGTGGT ATGTCTGGGT 
TCTTTGTATA AGAAGTTGGG GAGACTATTG GGTAACACCT TTATAGATAC AGTGGGGAAT 
ATACTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGTCGAT ATGAGATTAT GCTGAGTTTG 
CAAAATATAC TGAGTGGACT TGGAGCTGCT GCTGCCCCTT GTCACAGAGA CATTTATAAA 
GCTGCAAGGT CATGTTTGAC AGACAGGTCC ATGGCAGTTC GCTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAGAATGA AGCTAGCTTT ATGTGGAGTA CAGACATAGA CAGTGTTGCA 
ACTTTGTGTT TTAAGTCATT TGAAGGTTCC AACTATGATG TGCGAATAGC AGTATCAAAG 
CTCTTAGGTA CAGTGTTGGC TAGAGCTGTA ATGTCCAAAC CTGGAACAGC AACTTCCCGG 
CAAAATGTTC GCAAAGTTTC TCTGGAGGAA GCCATGGAGT TACTAGGAAC AGGGTTTCTA 
CGTGGGAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGT TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG TGTTACTCAG GCATATGTGG TGTTTATTTC AACACTAGGA 
GGACGTTGGT TAGAAAGAAA TTTTTCCATC TTACTCTCTC ATATCCTTAG CCTAGTATCA 
CAGTTTCACC CTAAAGCAAT CCAAACTCAG ATGGATGCTA TCTGTTGTCG TCGCTGTGTT 
TCATTTATCC TTCGAACTAC TATAGGAAGC CTTCTCGGGG AAAAAGCTCA AATTGCTGCT 
GCCAGGGATA TTTGTCAGGT CATCTGGAAA CTAAAGAAAA CTGTTGATGC TGTAATGAGT 
GAAGTTAATT TGGAAACTCG ACCTGGCTCC ACAGATGTTG CTGCCAGCCA GCATGTGCTA 
GTATGTGCTT TACAAGAACT TGGAAATCTA ATATATGGTC TTGGCACAAC AGCTGCACCT 
TTATTACAGG ATTCAAGTGC AGGGATTCTT GACAATGTCA TTTCAGTTAT TGTTCATCCC 
AGCATTTCAG TTCGATTAGC AGCAGCTTGG TGTTTACACT GCATTGCTGT AGCATTGCCT 
TCCTACCTAA CTCCACTTTT GGACAGTTGC CTTGAACGAC TTACTGTGCT CAAATCCTCA 
CCTGAAGCTG TGACTGGCTT TAGTTTTGCT GTGGCAGCTC TACTAGGAGC AGTGAAATAT 
TGCCCTTTAG GAATTCCACA TGGAAAAGGA AAGATAATTG TGATGTTAGC AGAGGATTTG 
TTATGTTCTG CTGCTCAGAA TAGTCGCCTT TCAGTTCAAC GAACACAAGC TGGATGGTTG 
CTGATAGCTG CCTTAATGAC ATTAGGCTCT GCATTTATTC ATCACCACCT TCCCCGGGTG 
CTAAGATTAT GGAAATGTGT TTTTCCACTA TCTCCTAAGG ATCTAGAAAC AGAAAGAAGT 
CGAGGAGACT CATTTACATG GCAGGTGACC TTGGAAGGTC GTGCAGGGGC CTTCTGTGCT 
ATCAAGAGCT TTGTTTCCCA CTGTAATGAT CTTCTTACTG ATGAAGTCAT TCAGAGACTT 
CTTCCTCCCC TTCCGTGTGC TGTTAATTTA TTAACTCAAC TTTCTTCAAT ACAGAAAACA 
TATGGAAGTC CTTTAAAAAC TCCTTCAGTG GTTTACAAAC AAAGACTTTA TGAACTATTG 
GCTTTATTAC CTCCTGAGAT CTATGCAGGA AGCCTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACCTGA CTTCTTCTGA TACCCAGATG GCAGCATCCA CTTTCTTACT TCCACCCCTC 
TGTCACCAAG ATGATCTTTT ATTACTAAGT CCTTTGCTAC AAGACACTGA CCACAGATTT 
ATTGAAGAAC AGCTCCTCCT TGGTAATGGT GTTGCTTGTG GAAGTCTAGA GTATGACCCA 
TTTTCAGTTT ATGAACATGG CATAGAAGGG GATTCAGTAC CTAAGCCCCT ACCTCCAATG 
TTGTCACTTA TCAGCTCTGC AGCCAGGCTT TTTGGAATTG TATGTGCCCA TGTAGCAGAA 
TCTCAAAGGC TCTTGGTATT GGAACAACTT TTGGACAAAA TAAAACACAC AAAAGCATCT 
CGTCAACAAG TAGTACAATT ACATGTTATG GCAGCATTTT CTAATTTTTT GAAGTATGTG 
GCCAACTCCA AAGGAAATTT AGGCTCAGAA GAAATTAGAA GACCTGCACT AACATTATTG 
ATGGGAGCCT TGGAAAGTAC CAACCCCTTA ATGCGTTGTA CTGCTGCAGA AGGTTTGGCT 
AGATTAGCCC AAGTGATTGG TGATGGCTCC TTCACTGCTG GATTAGCACA GGTTAGCTTT 
GAAAAACTGA AATCAGCTAG GGATGTGGTA ACCAGAACAG GACATTCTTT GGCTCTGGGA 
TCCTTACATA GATATTTAGG AGGAGTGGGC TCTTCTCAGC ACTTAAATGC TTGCATCGGA 
ATACTTTACA CTCTGTCACA GGATGGAACT TCTCCTGATG TACAGACATG GGCCCTACAT 
TCTCTCTCAT TGATAATTGA TTCTGCTGGT CCACTGTACC ATTTACATGT GGAATCTACC 
CTCTCTCTTA TACTAATGTT GTTGTTGACT GTTCCACCTA CTCATGCTGA AGTTCACCAA 
AGTCTTGGAC GATGTTTGAA TGTACTCATC ACCACATTGG GTCCAGAACT ACAAGGTAGC 
AGTACTGCAG TTTCTACTTT ACGGACATCC TGTCTTTTGG GGTGTGCAAT AATGCAGGAT 
AACCCAGATT GCCTTGTTCA AGCTCAAGCC ATCTCTTGTC TTCAACAACT TCATATGTTT 
GCCCCACGCT ATGTCAATTT ATCTAGTCTA GTCAGCTGTC TTTGTGAGAT CTTGTTGGAT 
AATTCTGTGT TGGTGAATCT CTGTAGCTCC TACTTGTTTC TGAGAAGAGC AGTACTAGCT 
TGTTTACGTC AAATTGTGCA AAGGGAAGCA GCTGAGGTTT CAGAACATGC TGTTGTTCTT 
GCTAAGGACA GCAGAGAAGA CTTAATTCCA GATGCTAATA TCCGAGAAGT GGGTCTTGAG 
GGGGCATTGT TGAACTTACT AGACAAAGAA TCTGATCAGA GATTGTGCCA GGATATCAAA 
GAGACCCTGA GTCATATGAT CACATCAATG GCAGTGGGAA AGCTTTCTTT TTGGCTAAAA 
CTTTGTAAAG ATGTCCTTGC TTCATCAGCT GATTTTACAA CTGTTGCTTC TGTGGATACA 
ACTCAGGATG ATGAAATAGA TAAAGAAGAT GATGCTTCAG TCCTAACCTA CAGAAATGAT 
GAGAAATGCC GGCCAATTAC AAATCCACGA TGGGCTACTA GAGTTTTTGC TGCTGAATGT 
GTGTGTAAAA TAATCAGCCA GTGTGAGAAT TCAGACAGTG CTCACTTTGA CATTGCTTTG 
GCTCAAGAAA TGAAAGAAAG GGATTCACGA AATGATTTTT TGGTATTACA TCTTGCTGAC 
TTGATCCGCA TGGCTTTTAT GGCTGCAACA GACCACAGTG ACCAGCTCCG TCTTTCTGGT 
CTTCAGACAC TGTTAGTAGT TGTTCGGCAA TTTGCAACTA TTCCTGAACC AGAATTCCCA 
GGTCATGTGA TTCTGGAACA GTATCAAGCC AATGTGGGAG CAGCACTTAG ACCAGCTTTT 
ACTTCAGATA CACCACCAGA TGTAACTGCC AAAGCATGTC AGGTTTGCAG TGCCTGGATA 
GCAAGTGGAG TTGTAAGTGA TCTTAATGAT CTCCGAAGAG TTCATCAATT GCTTGTATCA 
TCCTTGACGA AAATACAGGC TGGAAAAGAA GCCTTGAGTC AATTATATAA CGAAAGTGCT 
TCTACTATGG AAATCCTAGC TGTTCTCAAA GCCTGGGCAG AGGTTTACAT AATTGCTGTA 
GAAAGACAGA AAAAACAAAC TGAGAAACAG AAGCAGACTA TGAAAATTGT TGGCTCTATA 
GAGGAAAATG CCAGAGATGT GTCATCTTCC ATAGATGGAC TTCTTGACTT AGTCCAAACA 
GATCTTGGAA CATTAAGCAA ACTCTGGCTT GCTGCTCTTC AAGATTTTGC TCTTCTAACT 
TTGCCTTCAG AATTTGCTTC CCAACTTCCT CTTGAAGGTG GAGCTTTCTA CACTGCAGAA 
ACTAGTGAAA ATGCAAAGCC ACACTATCAT AATTCGTGGG CTCTTATTCT TCATGCTACA 
GCATTGTGGC TCACTAGCAC AGGCTTCATT GTAGCTGACC CAGATGAAGG AGCAACTAAT 
CTCTCAAGAC CTGTAACACC GACTACAATG TGTCAGGGTT CTTCTGGGGC ATCAGTGAAA 
TCCCCTGAGG ATGTTTATAC AGACAGATTT CACCTTATCT TGGGAATCAG TGTGGAATTT 
CTCTGTTCCC TGCGTTCAGA TGCAACCATG GAAAGCATCA CTGCTTGTTT ACATGCACTG 
CAGGCTTTAC TGGATGTTCC TTGGCCCAGA TCTAAGATTG GCAGTGACCA GGACTTGGGA 
GTTGAGTTGT TGAATGTACT CCATCGAGTA ATACTGACTA GAGAGTCACC TTCCATTCAG 
TTAGCTTCAC TTGAAGTTGT AAGACAGGTT ATCTACGCAG CCCAAGAACA TGTAAAGGAA 
AAGAGGAGAA GTGCAGAAGT TGATGATGGA GCTGCTGAAA AGGAAACCCT GCCAGAATTT 
GGAGAAGGAA AGGATACGGG AGGACTGGTA CCTGGAAGAT CTCTGGTGTT TGCAACATTG 
GAGCTATGTG TATGCATTTT GGTCCGGCAA CTTCCAGAGT TAAATCCTAA ACTGACCGGT 
AGTCCAGGAA TAAAAACTAC AAAAGCACAG ATTCTTTCAG ATGATGGAAA TAAGTTGGTT 
TCAGCAGCAT TAACTATCCT CTCAGAACTT CCTGGAGTAT GCTCTCCCGA AGGAAGCATT 
TCAGTTCTCC CTACTATTCT GTACCTTATA ATTGGGGTCC TCCGAGAAAC TGCTGTGAGG 
TTACCTGGAG GACAGTTATC TTATACTGTT GGAGCTGCTC TACAAGCCTT GAAAGGAATA 
TTGGCATCTC CCTTGGCTCG AGCAGAGAAG AGTCACACTG CTTGGACTGA CCTTCTTCGA 
AGTGCTTTGA CAACAATCCT AGACTGCTGG GATCCAGATG GAACACAGCA AGAGTTTGAT 
GAAATCAGCC TGCTTACTGC CATAACAGTA TTTATCTTGT CTTCAAGTCC AGAAGTGACC 
ACCATCCAGT GCCTGCAAGA ACGTTGTATA CAGAAGTTTA AAGCTACTCT TGAGTTAAAA 
GACCCAGTAG TACAAATAAA GTGCTATCAG CTACTGCTTT CCATTTTCCA GTATCCAAAC 
CCAGCAGTAT CCTACCCTTA TATTCATTCA TTAGCATCAT CTATTGTGAG AAAACTGCAA 
GAGACAGATA AAGTAAAACC AGAAGATGCT GCTGAGCTTC AGGTGATTCA AGAAGGTATT 
AAGGTCTTAG AAGCCCTAGT AGGGATTGCG GAAGAACAGC ACCGTGCTCA CTTGGTGGCC 
GACATTCTCC CTATTCTTAT TTCCTTTCTT TTGGATGAAA ACACTCTGGG CTCAGCACCC 
ACCACAATGA GAAATTTACA TGACTTTGCT TTGCAGAATC TGATGCAGAT TGGTCCCCAG 
TATTCATCAA CTTTTAAAAA TATTATGGCT TCTTCTCCCA CAATGAAAGC CCGTCTTGAG 
GCAGCTATAA AAGGCAATCA GGAAAATATC AAAATCAAGA CAACCCCAAA ACATATCACA 
AAACCTGGAA AAAATTCAAG TATTCAGTTG AAGACCAATT TTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031808120.1
CDS	139..6306
Translation	MELAHSLLLNEEAYNQLGEVQKAEFVFEWLKFLEKLLLATNKADIKEKQKSIIEQLISLLNSSPGPPTRKLLAENLAVLYSIGDTFSVYQTIDKCNELIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRLLGNTFIDTVGNILKAMKSAESQGRYEIMLSLQNILSGLGAAAAPCHRDIYKAARSCLTDRSMAVRCAAAKCLLELQNEASFMWSTDIDSVATLCFKSFEGSNYDVRIAVSKLLGTVLARAVMSKPGTATSRQNVRKVSLEEAMELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFISTLGGRWLERNFSILLSHILSLVSQFHPKAIQTQMDAICCRRCVSFILRTTIGSLLGEKAQIAAARDICQVIWKLKKTVDAVMSEVNLETRPGSTDVAASQHVLVCALQELGNLIYGLGTTAAPLLQDSSAGILDNVISVIVHPSISVRLAAAWCLHCIAVALPSYLTPLLDSCLERLTVLKSSPEAVTGFSFAVAALLGAVKYCPLGIPHGKGKIIVMLAEDLLCSAAQNSRLSVQRTQAGWLLIAALMTLGSAFIHHHLPRVLRLWKCVFPLSPKDLETERSRGDSFTWQVTLEGRAGAFCAIKSFVSHCNDLLTDEVIQRLLPPLPCAVNLLTQLSSIQKTYGSPLKTPSVVYKQRLYELLALLPPEIYAGSLCAILKELAADLTSSDTQMAASTFLLPPLCHQDDLLLLSPLLQDTDHRFIEEQLLLGNGVACGSLEYDPFSVYEHGIEGDSVPKPLPPMLSLISSAARLFGIVCAHVAESQRLLVLEQLLDKIKHTKASRQQVVQLHVMAAFSNFLKYVANSKGNLGSEEIRRPALTLLMGALESTNPLMRCTAAEGLARLAQVIGDGSFTAGLAQVSFEKLKSARDVVTRTGHSLALGSLHRYLGGVGSSQHLNACIGILYTLSQDGTSPDVQTWALHSLSLIIDSAGPLYHLHVESTLSLILMLLLTVPPTHAEVHQSLGRCLNVLITTLGPELQGSSTAVSTLRTSCLLGCAIMQDNPDCLVQAQAISCLQQLHMFAPRYVNLSSLVSCLCEILLDNSVLVNLCSSYLFLRRAVLACLRQIVQREAAEVSEHAVVLAKDSREDLIPDANIREVGLEGALLNLLDKESDQRLCQDIKETLSHMITSMAVGKLSFWLKLCKDVLASSADFTTVASVDTTQDDEIDKEDDASVLTYRNDEKCRPITNPRWATRVFAAECVCKIISQCENSDSAHFDIALAQEMKERDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLQTLLVVVRQFATIPEPEFPGHVILEQYQANVGAALRPAFTSDTPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSQLYNESASTMEILAVLKAWAEVYIIAVERQKKQTEKQKQTMKIVGSIEENARDVSSSIDGLLDLVQTDLGTLSKLWLAALQDFALLTLPSEFASQLPLEGGAFYTAETSENAKPHYHNSWALILHATALWLTSTGFIVADPDEGATNLSRPVTPTTMCQGSSGASVKSPEDVYTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGVELLNVLHRVILTRESPSIQLASLEVVRQVIYAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGRSLVFATLELCVCILVRQLPELNPKLTGSPGIKTTKAQILSDDGNKLVSAALTILSELPGVCSPEGSISVLPTILYLIIGVLRETAVRLPGGQLSYTVGAALQALKGILASPLARAEKSHTAWTDLLRSALTTILDCWDPDGTQQEFDEISLLTAITVFILSSSPEVTTIQCLQERCIQKFKATLELKDPVVQIKCYQLLLSIFQYPNPAVSYPYIHSLASSIVRKLQETDKVKPEDAAELQVIQEGIKVLEALVGIAEEQHRAHLVADILPILISFLLDENTLGSAPTTMRNLHDFALQNLMQIGPQYSSTFKNIMASSPTMKARLEAAIKGNQENIKIKTTPKHITKPGKNSSIQLKTNFF

Target ORF information:

RefSeq Version	XM_031952260.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 5A (HEATR5A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031952260.1

ORF Insert Sequence:

ATGGAACTGG CTCACAGCTT ATTGCTGAAT GAAGAAGCAT ATAATCAACT TGGAGAAGTT 
CAGAAGGCAG AATTTGTTTT TGAATGGTTG AAATTTTTAG AGAAACTTTT GCTAGCAACC 
AACAAGGCTG ACATAAAGGA GAAACAGAAG AGTATTATTG AGCAGCTCAT ATCTCTATTA 
AATAGCTCTC CAGGGCCACC TACCCGAAAG CTTCTTGCTG AGAACCTAGC TGTTCTTTAT 
AGTATTGGAG ATACATTTTC TGTTTATCAA ACAATTGACA AATGTAATGA ACTCATTCGT 
AGCAAAGATG ACTCTCCAAG TTATCTTCCC ACAAAACTTG CTGCTGTGGT ATGTCTGGGT 
TCTTTGTATA AGAAGTTGGG GAGACTATTG GGTAACACCT TTATAGATAC AGTGGGGAAT 
ATACTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGTCGAT ATGAGATTAT GCTGAGTTTG 
CAAAATATAC TGAGTGGACT TGGAGCTGCT GCTGCCCCTT GTCACAGAGA CATTTATAAA 
GCTGCAAGGT CATGTTTGAC AGACAGGTCC ATGGCAGTTC GCTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAGAATGA AGCTAGCTTT ATGTGGAGTA CAGACATAGA CAGTGTTGCA 
ACTTTGTGTT TTAAGTCATT TGAAGGTTCC AACTATGATG TGCGAATAGC AGTATCAAAG 
CTCTTAGGTA CAGTGTTGGC TAGAGCTGTA ATGTCCAAAC CTGGAACAGC AACTTCCCGG 
CAAAATGTTC GCAAAGTTTC TCTGGAGGAA GCCATGGAGT TACTAGGAAC AGGGTTTCTA 
CGTGGGAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGT TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG TGTTACTCAG GCATATGTGG TGTTTATTTC AACACTAGGA 
GGACGTTGGT TAGAAAGAAA TTTTTCCATC TTACTCTCTC ATATCCTTAG CCTAGTATCA 
CAGTTTCACC CTAAAGCAAT CCAAACTCAG ATGGATGCTA TCTGTTGTCG TCGCTGTGTT 
TCATTTATCC TTCGAACTAC TATAGGAAGC CTTCTCGGGG AAAAAGCTCA AATTGCTGCT 
GCCAGGGATA TTTGTCAGGT CATCTGGAAA CTAAAGAAAA CTGTTGATGC TGTAATGAGT 
GAAGTTAATT TGGAAACTCG ACCTGGCTCC ACAGATGTTG CTGCCAGCCA GCATGTGCTA 
GTATGTGCTT TACAAGAACT TGGAAATCTA ATATATGGTC TTGGCACAAC AGCTGCACCT 
TTATTACAGG ATTCAAGTGC AGGGATTCTT GACAATGTCA TTTCAGTTAT TGTTCATCCC 
AGCATTTCAG TTCGATTAGC AGCAGCTTGG TGTTTACACT GCATTGCTGT AGCATTGCCT 
TCCTACCTAA CTCCACTTTT GGACAGTTGC CTTGAACGAC TTACTGTGCT CAAATCCTCA 
CCTGAAGCTG TGACTGGCTT TAGTTTTGCT GTGGCAGCTC TACTAGGAGC AGTGAAATAT 
TGCCCTTTAG GAATTCCACA TGGAAAAGGA AAGATAATTG TGATGTTAGC AGAGGATTTG 
TTATGTTCTG CTGCTCAGAA TAGTCGCCTT TCAGTTCAAC GAACACAAGC TGGATGGTTG 
CTGATAGCTG CCTTAATGAC ATTAGGCTCT GCATTTATTC ATCACCACCT TCCCCGGGTG 
CTAAGATTAT GGAAATGTGT TTTTCCACTA TCTCCTAAGG ATCTAGAAAC AGAAAGAAGT 
CGAGGAGACT CATTTACATG GCAGGTGACC TTGGAAGGTC GTGCAGGGGC CTTCTGTGCT 
ATCAAGAGCT TTGTTTCCCA CTGTAATGAT CTTCTTACTG ATGAAGTCAT TCAGAGACTT 
CTTCCTCCCC TTCCGTGTGC TGTTAATTTA TTAACTCAAC TTTCTTCAAT ACAGAAAACA 
TATGGAAGTC CTTTAAAAAC TCCTTCAGTG GTTTACAAAC AAAGACTTTA TGAACTATTG 
GCTTTATTAC CTCCTGAGAT CTATGCAGGA AGCCTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACCTGA CTTCTTCTGA TACCCAGATG GCAGCATCCA CTTTCTTACT TCCACCCCTC 
TGTCACCAAG ATGATCTTTT ATTACTAAGT CCTTTGCTAC AAGACACTGA CCACAGATTT 
ATTGAAGAAC AGCTCCTCCT TGGTAATGGT GTTGCTTGTG GAAGTCTAGA GTATGACCCA 
TTTTCAGTTT ATGAACATGG CATAGAAGGG GATTCAGTAC CTAAGCCCCT ACCTCCAATG 
TTGTCACTTA TCAGCTCTGC AGCCAGGCTT TTTGGAATTG TATGTGCCCA TGTAGCAGAA 
TCTCAAAGGC TCTTGGTATT GGAACAACTT TTGGACAAAA TAAAACACAC AAAAGCATCT 
CGTCAACAAG TAGTACAATT ACATGTTATG GCAGCATTTT CTAATTTTTT GAAGTATGTG 
GCCAACTCCA AAGGAAATTT AGGCTCAGAA GAAATTAGAA GACCTGCACT AACATTATTG 
ATGGGAGCCT TGGAAAGTAC CAACCCCTTA ATGCGTTGTA CTGCTGCAGA AGGTTTGGCT 
AGATTAGCCC AAGTGATTGG TGATGGCTCC TTCACTGCTG GATTAGCACA GGTTAGCTTT 
GAAAAACTGA AATCAGCTAG GGATGTGGTA ACCAGAACAG GACATTCTTT GGCTCTGGGA 
TCCTTACATA GATATTTAGG AGGAGTGGGC TCTTCTCAGC ACTTAAATGC TTGCATCGGA 
ATACTTTACA CTCTGTCACA GGATGGAACT TCTCCTGATG TACAGACATG GGCCCTACAT 
TCTCTCTCAT TGATAATTGA TTCTGCTGGT CCACTGTACC ATTTACATGT GGAATCTACC 
CTCTCTCTTA TACTAATGTT GTTGTTGACT GTTCCACCTA CTCATGCTGA AGTTCACCAA 
AGTCTTGGAC GATGTTTGAA TGTACTCATC ACCACATTGG GTCCAGAACT ACAAGGTAGC 
AGTACTGCAG TTTCTACTTT ACGGACATCC TGTCTTTTGG GGTGTGCAAT AATGCAGGAT 
AACCCAGATT GCCTTGTTCA AGCTCAAGCC ATCTCTTGTC TTCAACAACT TCATATGTTT 
GCCCCACGCT ATGTCAATTT ATCTAGTCTA GTCAGCTGTC TTTGTGAGAT CTTGTTGGAT 
AATTCTGTGT TGGTGAATCT CTGTAGCTCC TACTTGTTTC TGAGAAGAGC AGTACTAGCT 
TGTTTACGTC AAATTGTGCA AAGGGAAGCA GCTGAGGTTT CAGAACATGC TGTTGTTCTT 
GCTAAGGACA GCAGAGAAGA CTTAATTCCA GATGCTAATA TCCGAGAAGT GGGTCTTGAG 
GGGGCATTGT TGAACTTACT AGACAAAGAA TCTGATCAGA GATTGTGCCA GGATATCAAA 
GAGACCCTGA GTCATATGAT CACATCAATG GCAGTGGGAA AGCTTTCTTT TTGGCTAAAA 
CTTTGTAAAG ATGTCCTTGC TTCATCAGCT GATTTTACAA CTGTTGCTTC TGTGGATACA 
ACTCAGGATG ATGAAATAGA TAAAGAAGAT GATGCTTCAG TCCTAACCTA CAGAAATGAT 
GAGAAATGCC GGCCAATTAC AAATCCACGA TGGGCTACTA GAGTTTTTGC TGCTGAATGT 
GTGTGTAAAA TAATCAGCCA GTGTGAGAAT TCAGACAGTG CTCACTTTGA CATTGCTTTG 
GCTCAAGAAA TGAAAGAAAG GGATTCACGA AATGATTTTT TGGTATTACA TCTTGCTGAC 
TTGATCCGCA TGGCTTTTAT GGCTGCAACA GACCACAGTG ACCAGCTCCG TCTTTCTGGT 
CTTCAGACAC TGTTAGTAGT TGTTCGGCAA TTTGCAACTA TTCCTGAACC AGAATTCCCA 
GGTCATGTGA TTCTGGAACA GTATCAAGCC AATGTGGGAG CAGCACTTAG ACCAGCTTTT 
ACTTCAGATA CACCACCAGA TGTAACTGCC AAAGCATGTC AGGTTTGCAG TGCCTGGATA 
GCAAGTGGAG TTGTAAGTGA TCTTAATGAT CTCCGAAGAG TTCATCAATT GCTTGTATCA 
TCCTTGACGA AAATACAGGC TGGAAAAGAA GCCTTGAGTC AATTATATAA CGAAAGTGCT 
TCTACTATGG AAATCCTAGC TGTTCTCAAA GCCTGGGCAG AGGTTTACAT AATTGCTGTA 
GAAAGACAGA AAAAACAAAC TGAGAAACAG AAGCAGACTA TGAAAATTGT TGGCTCTATA 
GAGGAAAATG CCAGAGATGT GTCATCTTCC ATAGATGGAC TTCTTGACTT AGTCCAAACA 
GATCTTGGAA CATTAAGCAA ACTCTGGCTT GCTGCTCTTC AAGATTTTGC TCTTCTAACT 
TTGCCTTCAG AATTTGCTTC CCAACTTCCT CTTGAAGGTG GAGCTTTCTA CACTGCAGAA 
ACTAGTGAAA ATGCAAAGCC ACACTATCAT AATTCGTGGG CTCTTATTCT TCATGCTACA 
GCATTGTGGC TCACTAGCAC AGGCTTCATT GTAGCTGACC CAGATGAAGG AGCAACTAAT 
CTCTCAAGAC CTGTAACACC GACTACAATG TGTCAGGGTT CTTCTGGGGC ATCAGTGAAA 
TCCCCTGAGG ATGTTTATAC AGACAGATTT CACCTTATCT TGGGAATCAG TGTGGAATTT 
CTCTGTTCCC TGCGTTCAGA TGCAACCATG GAAAGCATCA CTGCTTGTTT ACATGCACTG 
CAGGCTTTAC TGGATGTTCC TTGGCCCAGA TCTAAGATTG GCAGTGACCA GGACTTGGGA 
GTTGAGTTGT TGAATGTACT CCATCGAGTA ATACTGACTA GAGAGTCACC TTCCATTCAG 
TTAGCTTCAC TTGAAGTTGT AAGACAGGTT ATCTACGCAG CCCAAGAACA TGTAAAGGAA 
AAGAGGAGAA GTGCAGAAGT TGATGATGGA GCTGCTGAAA AGGAAACCCT GCCAGAATTT 
GGAGAAGGAA AGGATACGGG AGGACTGGTA CCTGGAAGAT CTCTGGTGTT TGCAACATTG 
GAGCTATGTG TATGCATTTT GGTCCGGCAA CTTCCAGAGT TAAATCCTAA ACTGACCGGT 
AGTCCAGGAA TAAAAACTAC AAAAGCACAG ATTCTTTCAG ATGATGGAAA TAAGTTGGTT 
TCAGCAGCAT TAACTATCCT CTCAGAACTT CCTGGAGTAT GCTCTCCCGA AGGAAGCATT 
TCAGTTCTCC CTACTATTCT GTACCTTATA ATTGGGGTCC TCCGAGAAAC TGCTGTGAGG 
TTACCTGGAG GACAGTTATC TTATACTGTT GGAGCTGCTC TACAAGCCTT GAAAGGAATA 
TTGGCATCTC CCTTGGCTCG AGCAGAGAAG AGTCACACTG CTTGGACTGA CCTTCTTCGA 
AGTGCTTTGA CAACAATCCT AGACTGCTGG GATCCAGATG GAACACAGCA AGAGTTTGAT 
GAAATCAGCC TGCTTACTGC CATAACAGTA TTTATCTTGT CTTCAAGTCC AGAAGTGACC 
ACCATCCAGT GCCTGCAAGA ACGTTGTATA CAGAAGTTTA AAGCTACTCT TGAGTTAAAA 
GACCCAGTAG TACAAATAAA GTGCTATCAG CTACTGCTTT CCATTTTCCA GTATCCAAAC 
CCAGCAGTAT CCTACCCTTA TATTCATTCA TTAGCATCAT CTATTGTGAG AAAACTGCAA 
GAGACAGATA AAGTAAAACC AGAAGATGCT GCTGAGCTTC AGGTGATTCA AGAAGGTATT 
AAGGTCTTAG AAGCCCTAGT AGGGATTGCG GAAGAACAGC ACCGTGCTCA CTTGGTGGCC 
GACATTCTCC CTATTCTTAT TTCCTTTCTT TTGGATGAAA ACACTCTGGG CTCAGCACCC 
ACCACAATGA GAAATTTACA TGACTTTGCT TTGCAGAATC TGATGCAGAT TGGTCCCCAG 
TATTCATCAA CTTTTAAAAA TATTATGGCT TCTTCTCCCA CAATGAAAGC CCGTCTTGAG 
GCAGCTATAA AAGGCAATCA GGAAAATATC AAAATCAAGA CAACCCCAAA ACATATCACA 
AAACCTGGAA AAAATTCAAG TATTCAGTTG AAGACCAATT TTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HEATR5A cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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