RYR1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	RYR1
Entrez Gene ID	100916717
Full Name	ryanodine receptor 1
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023499964.1	XP_023355732.1	LOW QUALITY PROTEIN: ryanodine receptor 1
XM_012545654.1	XP_012401108.1	LOW QUALITY PROTEIN: ryanodine receptor 1
XM_031961617.1	XP_031817477.1	LOW QUALITY PROTEIN: ryanodine receptor 1

Pathways from BioSystems
Gene Ontology

The following RYR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RYR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa126995	XM_012545654.1 Latest version!	Sarcophilus harrisii ryanodine receptor 1 (skeletal) (RYR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa152378	XM_023499964.1 Latest version!	Sarcophilus harrisii ryanodine receptor 1 (RYR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa230812	XM_031961617.1 Latest version!	Sarcophilus harrisii ryanodine receptor 1 (RYR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RYR1 ( XM_012545654.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012401108.1 Sarcophilus harrisii ryanodine receptor 1 (skeletal) (RYR1), mRNA.

Price & Availability↑

CloneID	OSa126995
Clone ID Related Accession (Same CDS sequence)	XM_012545654.1
Accession Version	XM_012545654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	LOW QUALITY PROTEIN: ryanodine receptor 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831767.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases internal stop codons :: corrected 3 genomic stop codon ##RefSeq-Attributes-END##

ATGGGGTCCC TGTTAGAACC CATCTGTCTA AAACCGTCTC GGCTCCCGGC CCAAGAAGGG 
AAAGAGGAGC AGCAGGGCTA CGGGGAAGAT GAGGTTGTCC TGCAGTGCAG CGCTACCACC 
CTCAAGGAGC AAATCAAGCT CTGCCTAGCC GCCGAAGGCT TTGGAAACCG ACTCTGTTTT 
TTGGAACCTA CCAGCAATGC CCAGAATGTG CCCCCTGACT TGGCTGTGTG CTGCTTTGTG 
CTGGAACAAT CTCTCTCGGT GCGAGCCCTG CAGGAGATGC TGGCCAACAC AGTGGAGGCT 
GGCACTGAGT CATCTCAGGG CGGGGGACAC CGGACCCTTC TCTATGGCCA CGCCATCTTA 
CTCCGGCATG CCCACAGCCA AATGTACCTA AGCTGCCTCA CAACATCCCG CTCTATGACT 
GACAAACTCG CCTTCGATGT GGGCCTGCAA GAAGATGCCA CAGGGGAGGC CTGCTGGTGG 
ACCATGCATC CGGCCTCAAA GCAGCGATCC GAAGGAGAGA AGGTCCGGGT GGGGGATGAC 
CTCATCCTTG TCAGTGTCTC TTCTGAAAGA TACTTGCACC TGTCCACAGC CAGTGGGGAG 
CTCCAGGCTG ATGCTTCTTT CATGCAAACC CTTTGGAACA TGAACCCCAT CTGTTCTGGG 
TGTGAGGAAG GGTACGTGAC CGGTGGCCAT GTTCTCCGGC TGTTCCACGG CCACATGGAC 
GAGTGTCTAA CCATCTCTTC AGCTGACAGT GATGACCAGC GCAGGTCTGG CCATGAGGGG 
CGGAGGGCCT GGATGCCAGG GGAATTAGGG AGTGGATGTC GTCACTGTGA GATGCTTGGG 
CATCTAGGTG ATTTAGGGTG GGACAGGGCT GCGGGCCCAG ACTCTAAGGG GTTGAGGGAT 
GGAATGCTGA CTTTGGTCCT GAATTGTATC GATCGGCTCA ACTTCTACTC CACGGCTGCC 
CACTTTGCTG AGTTTGCAGG AGAGGAGGCA GCTGAGTCTT GGAAAGAGAT TGTGAACTTA 
CTCTATGAGC TTTTGGCTTC TTTGATTCGG GGAAACCGTA CCAATTGTGC TCTCTTCTCC 
CACAACCTCG ACTGGCTTGT CAGCAAGTTG GATCGGCTCG AGGCCTCTTC TGGAATCCTG 
GAGGTCCTTT ATTGTGTACT GATTGAGAGT CCAGAAGTAC TGAACATTAT TCAGGAGAAT 
CACATCAAGT CCATCATTTC CCTGTTGGAC AAGCATGGCC GGAACCACAA GGTTCTGGAC 
GTGTTGTGCT CTCTGTGCGT GTGTAATGGC GTTGCTGTCC GCTCCAACCA GGTCCTGATC 
ACCGAGAACC TCCTTCCCGG GCGTGAGCTC CTACTACAGA CCAACCTCAT TAACTATGTC 
ACCAGCATAC GCCCTAATAT CTTCGTGGGC CGGGCTGAGG GGACCACACA GTATGGGAAG 
TGGTACTTTG AAGTCATGGT AGACTCTGTG GAACCTTTCC TGACTGCTCA AGCCACACAC 
CTGAGAGTGG GCTGGGCCCT CACTGAGGGA TACAGCCCCT ACCCTGGTGG TGGGGAAGGC 
TGGGGTGGCA ATGGCGTTGG AGATGACCTC TACTCCTATG GCTTTGATGG ACTGCACCTC 
TGGTCTGGGA GGATTGCCCG GCCAGTGGCC TCTCCAGGCC AGCACCTCCT GGCTGCCGAG 
GATGTAGTTA GCTGCTGTCT GGATCTGAGT GTCCCATCCA TCTCATTCCG AATCAATGGC 
TGCCCCGTGC AAGGCGTCTT TGAGGCTTTC AACCTGGATG GGCTCTTCTT CCCAGTGGTC 
AGCTTCTCTG CTGGTGTTCG GGTACGGTTT CTCCTGGGTG GTCGCCATGG AGAGTTCAAA 
TTCCTCCCCC CACCGGGCTA TGCCCCATGC CATGAAGCTC TACTACCCCG TGAGCGCCTC 
CGGCTGGAGC CCATTAAGGA GTACCGTCGA GAAGGACCCC ATGGGCCTCA CCTCTTGGGA 
CCTACTTGCT GCTTGTCCCA CACCGACTTT GTGCCCTACC CTGTGGACAC TGTCCAGATT 
GTTTTGCCAC CTCATCTGGA ACGGATCCGA GAGAAACTGG CAGAGAATAT CCATGAGCTC 
TGGGCCCTGA CTCGCATCGA GCAAGGATGG ACCTATGGCC CGGTACGGGA TGATAACAAG 
CGGCTTCACC CATGCCTGGT GGACTTTCAC AGCCTCCCAG AGCCTGAGAG AAACTACAAT 
CTGCAGATGT CAGGAGAGAC ACTCAAGACC CTGCTGGCTT TGGGATGTCA TGTAGGCATG 
GCTGATGAGA AGGCTGAGGA AAACCTCAAG AAGACAAAAC TCCCCAAGAC GTACATGATG 
AGNNNNNNNN NNNNNNNNNN NNNNNTGGAC CTGAGCCACG TGCGGTTAAC CCCAGCACAG 
ACGACGCTCG TTGACCGGCT GGCTGAGAAT GGGCACAATG TGTGGGCGAG AGACCGTGTG 
GGGCAAGGCT GGAGTTAAAG TGCTGTCCAA GACATCCCTG CCCGGAGGAA CCCCCGGCTT 
GTCCCCTACC GCCTCCTGGA TGAGGCTACC AAGCGCAGCA ATCGAGACAG CCTCTGCCAA 
GCAGTGCGCA CCCTCTTGGG TTATGGCTAT AACATTGAGC CACCTGACCA GGAATCCAGC 
CAAGTGGAGA GCCAGACCAG TGGGGACCGA GTACGCATCT TCCGAGCAGA ACGCAGCTAC 
GCTGTTCAGA GCGGTCGCTG GTATTTTGAG TTTGAGGCCG TGACCACTGG AGAAATGCGT 
GTGGGCTGGG CCCGTCCAGA GCTGCGGCCA GACGTGGAGC TGGGAGCAGA TGATTTGGCT 
TATGTCTTCA ATGGCCACCG AGGGCAGCGC TGGCACATGG GCAGTGAGCC ATTTGGACGA 
CCCTGGCAAC CAGGCGACGT GGTTGGGTGT ATGATCGACC TTTCGGAGAA CAACATCCTC 
TTCACACTCA ATGGAGAAGT GCTCATGTCT GACTCGGGCT CGGAGACGGC CTTTAAGGAT 
ATTGAGATTG GGGATGGCTT CCTCCCTGTC TGCAGCCTGG GCCTGGGCCA GGTGGGACGC 
CTAAATTTGG GCCAGGATGT GGGATCTCTT CGCTATTTCA CCATCTGTGG ACTTCAGGAG 
GGCTTTGAAC CTTTTGCCAT CAACATGCAG CGGCCTGTCA CCACCTGGTT CAGCAAGAGC 
CTGCCCCAGT TTGAACCGAT GCCCCCTGAC CATCCCCACT ATGAGGTGAC CCGAGTGGAC 
GGCACGGTGG ACACGCCTCC CTGCCTGCGC CTGACGCACC GTACCTGGGG CTCCCAGAAC 
AGCCTGGTGG AGATGATGTT CCTGAGGCTG AGCCTCCCGG TGCAGTTCTA TCAGCACTTC 
CGCTGCACCC CGGGGGCCAC CCCCTTGGCC CCGCCGGGTC CCCAGCCCCC CGGGGACGAC 
GAGGTCCGAG CGGCCGAGCC TGACCCCGAC TACGAGCAGA TGCAGCGTTC GGCGGGCCGG 
ACGGGCGAGG CTGAGGGGAA CAAGGAGGGG ACTGGGGGGC CCACCAAGGA CGGGGCGGCT 
TCTCAGNNNN NNNNNNNNNN NNNNNNGAAG GACGCTGCCA CTGAGAAAAG CAAGAAGAGG 
GGGTTCTTGT TCAAAGCCAA GAAGGCAGCC ATGATGACGC AACCACCCGC CACCCCCGCT 
CTGCCCCGAC TCCCTCACGA CGTGGTGTCC GCGGACAACA GGGATGACCC TGAGATTATC 
CTCAACACCA CCACGTATTA CTACTCTGTC CGGGTGTTTG CCGGCCAGGA GCCCAGCTCA 
GTGTGGGTGG GCTGGGTCAC CCCTGACTAC CACCAGCACG ACATGAATTT TGATCTGACC 
AAAGTCCGCG TTGTGACGGT GACCATGGGG GACGAGCGAG GCAATGTACA CAACAGCTTG 
AAGCGCAGTA ACTGCTACAT GGTGTGGGGT GGGGATTTTG TGAGCCCGGG ACAGCAAGGA 
CGCATCAGTC ACACAGACCT GGTCATCGGC TGCCTGGTGG ACCTGGCCAC CGGCCTCATG 
ACCTTCACGG CCAATGGAAA AGAGAGCAAC ACCTTTTTCC AGGTGGAGCC AAACACGAAG 
CTGTTTCCAG CGGCCTTCGT CCTTCCCACC AACCAAAATG TTATGCAATT TGAACTGGGA 
AAGCTGAAGA ACATCATGCC CCTGTCAGCA GCCATGTTCC TGAGTGAACG GAAGAACCCA 
GCGCCGCAGT GTCCCCCCCG CTTGGAGGTA CAAATGCTCA TGCCCGTGTC CTGGAGCAGG 
ATGCCCAACC ACTTCCTACA AGTGGAGACA CACCGGGCAG GTGACCGCCT GGGCTGGGCT 
GTGCAGTGTG AGGAGCCTCT CACCATGATG GCCTTGCATA TCCCTGAGGA GAACCGGGGA 
GGCCATTGGC CTACGATGGT GGGAGCCTCG ACTTCCACAC CGAGGGACCT GGAACTGGTC 
CTGCAGGTGA TGGGCATCTT CGGGGACGAG GACGTCAAGC AAATCCTGAG GATGATTGAG 
CCTGAAGTTT TCAAGGAAGA GGAAGAGGAG GAAGAGGAGG AGGAGGAGGA GGAGGATGAA 
GAAGAGAAGG AAGAGGATGA AGAAGAAGAG GAAGAAGAAA AAGAGGAGGA GGTGAAAGAG 
GAGGAGGAGG AAGAGGAAGA GAAGGGAGAG GAAGAAGAAG CTGTGGAGGA AGGACTACTT 
CAGATGAAGC TGCCTGAGTC TGTCAAGCTG CAGATGTGCC ATCTGCTGGA GTACTTCTGT 
GACCAGGATC TGCAGCACCG AGTGGAGGCG CTCATTGCGT TCTCGGAGCG CTACGTCAGT 
TGCCTGCAGA ACAACCAGCG CAGCCGCTAC GGGCTGCTCA TAAAGGCCTT CACTATGTCC 
GCAGCGGAGA CTGCCCGGCG CACCCGGGAG TTTCGCTCTC CGCCCCAGGA GCAGATCAAC 
ATGCTTCTGC ACTTCAAAGG GAGTGAAGAC GAGGAGGACT GTCCGGTCCC AGACGAAATT 
CGGCAGGAGC TGCTGGATTT TCACCAGGAC CTGCTGCTGC ACTGCGGCAT CCAGCTGGAA 
ACGGAGGAGG AGGAGGTGGA AGAGGAGACC ACCCTGGGAG GCCGGCTCAT GAGCTTTTTG 
GAGAAGGTGA AGCTGGTGAA GAAGAAGGAG AAGGAGCCAG ATGAGACCCC TCCCCCAGAA 
GAGAAAAAGC CTGAGTCCTT GCAGGAGCTG GTCTCTCACA CAATGGTGCG CTGGGCCCAA 
GAGGACTTTA TCCAGAACCC AGAGCTGGTC CGGGCCATGT TCAGCCTCCT CCACCGGCAG 
TACGATGGCC TCGGGGAGCT GCTCCGTGCC CTGCCCAGGG CTTACACCAT CTCCCCGTCC 
TCCGTGGAGG ACACCATGAG CCTCCTGGAA AGCCTGTGTC AGATCCGCTC CTTGCTCATT 
GTGCAGATGG GGCCGCAAGA GGAGAATCTC ATGATCCAGA GCATTGGGAA TATCATGAAC 
AACAAGGTGT TTTACCAGCA CCCAAATCTG ATGAGGGCTT TGGGGATGCA CGAAACAGTG 
ATGGAAGTTA TGGTCAATGT GTTAGGAGGG GGAGAGTCTA AGGAAATCCG CTTCCCCAAA 
ATGGTCACCA GCTGCTGCCG GTTCCTCTGC TATTTCTGTC GCATTAGTCG CCAGAACCAA 
AGGTCCATGT TTGATCACCT CAGCTACCTG CTGGAGAACA GCGGGATCGG CCTGGGTATG 
CAGGGTTCCA CTCCGCTAGA TGTGGCGGCT GCCTCCGTCA TAGACAACAA CGAGCTGGCC 
TTGGCCCTTC AGGAACAAGA CCTGGAAAAG GTCGTCTCCT ACTTGGCCGG CTGCGGCCTT 
CAGAGCTGCC CCATGCTGCT GGCCAAGGGC TACCCCGACA TTGGCTGGAA CCCCTGCGGG 
GGGGAGCGCT ATCTGGATTT CCTGAGATTT GCCGTCTTTG TTAATGGAGA GAGCGTGGAA 
GAGAATGCGA ATGTTGTGGT TCGTCTGCTC ATCCGGCGTC CCGAATGCTT CGGCCCGGCC 
CTGCGAGGAG AGGGCGGCTC TGGGCTGCTG GCTGCTATGG AAGAGGCAAT CCGGATCTCA 
GAGGATCCTG CTCGAGATGG CCCGGGCGTC AGGAAGGACC GGAGGCGGGA GCACCACGGG 
GGTGAAGAGG CCCCTGAAGA AAACCGTGTG CACTTGGGAA ATGCCATCAT GTCATTCTAT 
TCCGCCCTGA TCGACCTGCT GGGACGCTGT GCTCCAGAGA CTCATCTGAT CCAGGCCGGG 
AAGGGAGAAG CGCTGAGGAT CCGAGCCATT CTGCGCTCCC TGGTGCCCCT GGACGACCTC 
GTGGGCATTA TCAGCCTGCC CCTGCAGATC CCCACCCTGG GCAAAGATGG GGCCCTGGTG 
CAGCCTCGGA TGTCTGCCTC CTTCGTGCCT GACCACAAAG CCCCCATGGT CCTCTTCCTG 
GACCGCGTCT ACGGCATCGA CAGCCAGGAC TTCCTGCTCC ACGTGCTTGA TGTCGGCTTC 
CTGCCTGACA TGCGGGCGGC TGCCTCCCTG GACACGGCAA CCTTCAGCAC CACGGAGATG 
GCCCTGGCCT TGAACCGCTA CCTGTGCACA GCCGTGCTTC CCCTGATCAC CAAGTGCGCC 
CCACTGTTTG CGGGGACGGA GCACCGCGCC ATCATGGTGG ACTCCATGCT CCACACGGTG 
TACCGCCTGT CCCGGGGACG CTCTCTCACC AAGGCCCAGC GAGATGTCAT TGAGGACTGC 
CTCATGGCGC TCTGCAGGTA CATCCGTCCA TCTATGCTCC AGCACCTTCT GCGTCGTCTG 
GTATTCGACG TTCCCATCCT CAACGAGTTT GCCAAGATGC CCCTCAAGCT CCTGACCAAT 
CACTATGAGC GCTGCTGGAA ATATTACTGC CTCCCTACGG GCTGGGCCAA CTATGGAGTT 
ACCTCCGAGG AAGAGCTGCA TCTGACTCGC AAGCTTTTCT GGGGCATCTT TGAGTCACTG 
GCCCACAAGA AATACGACCA GGAGCTCTAC CGCATGGCCA TGCCGTGTCT GTGCGCCATC 
GCCGGGGCCC TGCCCCCCGA CTACGTGGAT GCCTCCTACT CCTCCAAAGC TGAGAAGAAG 
GCCACTGTGG ATGCTGAGGG CAACTTTGAC CCCCGGCCCG TGGAGACACT CAATGTGATC 
ATACCCGAGA AACTCGACTC CTTCATCAAC AAATTTGCCG AGTACACACA CGAGAAGTGG 
GCATTTGACA AGATTCAGAA CAATTGGTCA TTTGGGGAGA ACATTGACGA GGAGCTCAAG 
ACCCACCACA TGCTTCGGCC CTACAAGACC TTCTCAGAAA AGGACAAGGA GATTTACCGA 
TGGCCCATCA AGGAGTCCCT GAAGGCCATG ATCGCCTGGG AGTGGACTGT GGAAAAGGCC 
CGTGAGGGTG AGGAGGAGAA GGGAGAGAAG AAGACCAAGA CTCGCAAGAT CTCCCAGAAT 
ACCCCGGCCA CCTATGACCC AAGAGAGGGC TATAATCCCC AGCCTCCAGA CCTTACCTGT 
GTCACCCTGT CCCGGGAACT GCAGGCCATG GCTGAACAAC TGGCTGAGAA TTACCATAAC 
ACATGGGGGA GGAAGAAAAA GCAGGAATTA GAAGCCAAGG GTGGAGGCAC CCACCCCCTC 
CTGGTCCCCT ATGACACACT GACGGCCAAG GAGAAGGCTC GCGATAGAGA GAAAGCCCAG 
GAACTGCTCA AATTCCTGCA GATGAACGGC TACGCGGTGA CGCGGTGCCC CCAGGCCTTC 
CTCCATCTCC CCCGAGCCCT GTCCTGTTCC CCCAGGACCG TGATGAAGTC TGGTCCCGAG 
ATCGTCAAGG CTGGTCTCCG ATCGTTCTTC GAGAGCGCTT CGGAGGACAT CGAGAAGATG 
GTGGAGAACC TGCGCCTGGG CAAGGTGTCC CAGGCGCGGA CGCAGGTCAA GGGCGTCGGC 
CAGAACCTCA CCTACACCAC CGTGGCTCTG CTTCCCGTTC TCACCACGCT TTTCCAGCAC 
ATCGCCCAGC ACCAGTTTGG GGATGATGTC ATCTTGGACG ACGTCCAGGT CTCCTGCTAC 
CGAACACTTT GCAGCATCTA CTCTCTGGGG ACCACCAAGA ATTCCTATGT GGAGAAGCTC 
CGTCCAGCCC TTGGAGAATG CCTTGCCCGT CTGGCAGCTG CTATGCCAGT TGCCTTCCTT 
GAACCCCAAC TGAATGAGTT CAACGCCTGC TCTGTCTACA CCACCAAATC TCCCCGAGAA 
CGTGCCATCC TGGGCCTTCC GAGCTCCGTG GAGGAGATGT GCCCAGATAT CCCAGACCTG 
AAGCACCTGA TTGCCGAGAT CGGGGGGCGG GCCGAGTCGG GCGCCCGTTA CACCGAGATG 
CCCCATGTCA TCGAGATCAC GCTGCCGATG CTCTGTAACT ACCTGCCCCG CTGGACAGTG 
AATGACACAC AAGCCCACTG GCTAACAGAA CCCAACCCTG ATGCAGAAGA ACTCTTCAGG 
ATGATAGCAG AAATCTTCAT CTATTGGTCC AAGTCTCATA ACTTCAAGAG AGAAGAACAG 
AATTTCGTGG TACAGAACGA AATCAATAAC ATGTCCTTCC TGACGGCAGA TAGCAAGAGC 
AAGATGGCCA AGTCAGGAGG CTCCGATCAA GAGAGAACAA AGAAGAAACG GCGAGGGGAC 
CGATATTCCG TCCAGACCTC TCTGATCGTG GCTACCCTCA AGAAGATGCT GCCTATTGGG 
TTGAACATGT GTGCACCCAC TGACCAGGAC CTCATCGCGC TGGCCAAGAC TCGTTATGCC 
CTGAAAGACA CAGATGAAGA AGTTCGGGAA TTTCTGCAGA ATAACCTTCA TCTTCAGGGA 
AAGAGTGAGG GCTCCCCATC ACTGCGCTGG CAGATGGCCC TGTACCGGGG GCTGCCTGGC 
CGGGCTGAGG ATGCTGATGC CCCTGAGAAC ATTGTTCGCC GAGTACAGGA AGTCTCAGCG 
GTGCTTTACC ACCTGGAACA GACAGAACAT CCCTACAAGT CCAAGAAAGC TGTGTGGCAC 
AAGCTGCTTT CAAAACAGCG TCGTCGGGCG GTTGTGGCTT GTTTTCGCAT GACACCCTTG 
TACAACTTGC CTCGGCATCG GGCAAGCAAT ATGTTCTTGG AGTCCTACAA AGCATCATGG 
ATCCTAACTG AGGAGCACAG TTTTGAGGAC CGCATGATTG ATGACCTTTC TAAAGCTGGG 
GAGGAAGAAG AAGAGGAAGA AGAACAGGAA GAGAAGAAGC CAGACCCTCT TCACCAGCTG 
GTATTACACT TTAGCCGGAC AGCCCTGACA GAGAAGAGCA AACTAGATGA GGACTATTTG 
TACATGGCAT ATGCTGACAT CATGGCCAAG AGCTGCCACT TGGAGGAGGA AGAGGAGCCT 
TCAGAACCTG CTGAGGAGGA GGCTGAAGTT TCATTTGAGG AGAAGGAGAT GGAAAAGCAG 
AAACTGCTCT ACCAGCAGTC AAGGCTACAC AACCGAGGGG CTGCTGAGAT GGTGCTACAA 
ATGATTAGTG CCTGCAAAGG GGAGCCGGGA GTCATGGTTT CATCCACCCT GAAGTTGGGC 
ATCTCCATCC TCAACGGTGG CAACATGGAG GTGCAGCAGA AGATGCTGGA CTATCTGAAG 
GACAAAAAGG ATGTTGGCTT CTTCCAGAGC ATCCAGGCCC TGATGCAGAC ATGCAGGTCT 
GTCCTGTGCA GTGCAGGGTG ACAGGCTGGA TTGACAGAGG GATGGAGAAA TGGAGAGACA 
GGCTGACCCT CCCTCTCTGT CCCAGGGGAA AAAGTAATGG CCGACGATGA GTTCACCCAG 
GATCTCTTCC GCTTTCTGCA GCTGCTGTGT GAAGGCCATA ACAATGACTT TCAGAACTAC 
CTCCGCACGC AGACGGGGAA CACGACCACC ATCAACATCA TCATCTGCAC AGTGGATTAT 
CTGCTGAGGC TGCAGGAGTC CATCAGTGAC TTCTACTGGT ACTACTCGGG GAAGGATGTG 
ATCGAAGAGC AAGGGAAGAG GAACTTCTCC AAAGCCATGG CCGTGGCCAA GCAGGTCTTC 
AACAGCCTCA CCGAGTACAT CCAGGACTCA AGCCAGATTG AGCTGCTGAA GGAGCTCCTG 
GATCTGCAGA AAGACATGGT GGTCATGTTG CTTTCGCTGC TGGAAGGTAA CGTGGTCAAC 
GGCACAATTG CCCGGCAGAT GGTGGACATG CTGGTGGAGT CCTCCTCCAA CGTGGAGATG 
ATCCTCAAGT TCTTCGACAT GTTCCTAAAG CTGAAGGACA TCGTGGGCTC CGAGGCATTC 
CAGGACTACG TCACCGACCC CCGTGGACTC ATCTCCAAGA AGGATTTCCA GAAGGCCATG 
GACAGCCAGA AGCAGTTCAC TGGCCCAGAG ATCCAGTTCC TCCTGTCCTG CTCAGAGGCG 
GATGAGAACG AGATGATCAA TGTGGAGGAA TTTGCCAGCC GCTTCCAGGA GCCGGCCCGA 
GACATCGGCT TCAACGTGGC CGTGCTGCTG ACCAACCTGT CGGAGCACGT GCCCCACGAC 
CCGCGCCTCA AGAACTTCCT GGAGCTGGCT GAGAGCATCC TGGAGTACTT CCGGCCCTAC 
CTGGGCCGGA TCGAGATCAT GGGGGCCTCC CGGCGCATCG AGCGCATCTA CTTTGAAATC 
TCTGAGACCA ACAAAGCACA GTGGGAGATG CCCCAGGTGA AGGAGTCCAA ACGCCAGTTC 
ATCTTTGACG TGGTGAATGA AGGGGGGGAG TCGGAGAAGA TGGAAATGTT TGTGAGCTTC 
TGTGAAGACA CCATCTTTGA GATGCAGATC GCCGCCCAGA TCTCAGAGCC CGAGGGAGAG 
CCTGAGGAAG ATGAGGAGGA GTCCATGGCA GAGTCGGGGC CAGACTCCGG GGAGGCAGGG 
CCCGACGGAG AAGGGGCCGG CGCAGGGGGC CCGACGGGCA TCTCCGGGCA CGTGGCCGGT 
GCCGTCTCCT CGGTCCTGCG GAAGCTGACC TACCGGAGCC TGCGCAGGAG GGTGCGGAGG 
CTCCGCAAGC TGACCCTGCG GGAGGCTGCA GGAACCGGGG CCGGGCTCAT CTGGGCCGGG 
GCCACGTGGA CGGGCTCCGG AGTGATCGGC GCCACCCGGG GGGCCCTGGG CCTACTCTGG 
GGCTCTCTGT TTGGGGGAGG GCTGGTGGAG GGAGCCAAGA AAGTGACCGT GACGGGACTG 
TTGGCCGGGA TGCCAGACCC CACAGGCGAT GAGGTCCATG GGGAGCAGGC GGCTGGCGCC 
GGGGCTGCCG GGACTGGGGA CGGAGCCGTG GAGGGTGATG AGGAGGTGGG GGCGGAGTTG 
GCCGAGAGGG AGGCTGAGGC TGAGGCCGGA GCTGGGGAGG CCGGGCCAGC CGGAGGAGAG 
GGAGCCGGGG CCAATGGAGG CCACTTCCGG ATGGAGGTCC CCGGAGGCCT GGGTGACATG 
GGAGACACAA CACCCGTTGA GCCGCCCACT CCTGAGGGTT CCCCCATCTT CCGGAGGAAA 
CTAGGGCTGG AGGGAGAAGA AGAACAAGCC TTAGAACCAG AACCAGAGCC AGAACCAGAA 
CCAGAGCCAG AGCCAGAACC CGAGCCTGAG AAAGCTGATG CTGAGAACGG AGAAAAGGAA 
GGGTCCCAAG AGCCAGAACC TGAGGCTCCC AAGAAGGCAG CTGCCCCACC TCCGCCCCGG 
AAGGAGGACG CCAAATCTGA GGGCCCTGAC TTCTGGGAGG AAATGGAGGT GCAGAGGGTC 
AAGTTCCTGA ACTACCTCTC CAGAAACTTC TATACCCTGA GATTCCTCGC CCTCTTCCTG 
GCCTTTGCCA TCAACTTCAT CCTGCTCTTC TACAAGGTGT CTGACTCTCC GCCAGGAGAA 
GAGGACCTGG AAGGCTCCGG TCTCGAGGGT CTGGCAGGCT CAGGGTCCGG GGACTTCCTG 
GGATCCGGCA TGGGCTCCGG GGCTGGGGAG GATCTGGAGA ACGAGGAGGA AGAGAACATG 
GTCTACTACT TCCTGGAAGA GAGTACGGGC TACATGGAGC CAGCCCTGCG AGGTCTCAGC 
ATCCTGCACA CCCTCATTGC CTTCCTCTGC ATCATAGGCT ACAACTGTCT CAAGGTACCG 
CTGGTTATAT TTAAACGGGA GAAGGAGCTA GCCCGGAAAC TAGAGTTCGA TGGCCTTTAC 
ATCACTGAGC AGCCCGAGGA TGACGACGTG AAGGGCCAGT GGGATCGACT GGTGCTTAAC 
ACCCCGTCCT TCCCCAGCAA CTACTGGGAC AAGTTCGTGA AAAGGAAGGT GCTGGAGAAA 
CATGGCGACA TCTTTGGCCG GGAGCGGATT GCAGAGCTGT TGGGCATGGA TCTGGCCAGC 
CTGGAGATCA CGGCGCAGAA CGAGAAGCGA CCGGAACCCC CCTCGGGACT GCTCACCTGG 
ATCATGTCCA TAGATGTCAA ATACCAAATC TGGAAATTTG GTGTCATCTT TACAGATAAT 
TCATTCTTGT ATTTGACGTG GTACATGGTC ATGTCCCTCC TCGGCCACTA TAACAACTTC 
TTCTTTGCCG CTCACCTGCT GGACATCGCC ATGGGAGTCA AGACGCTTCG GACCATCCTG 
TCTTCTGTCA CCCACAATGG GAAGCAGCTG GTGATGACGG TGGGCCTGCT GGCCGTCGTG 
GTTTACCTCT ACACGGTTGT GGCCTTCAAT TTCTTCCGCA AATTTTACAA TAAAAGCGAG 
GATGAGGATG AGCCGGACAT GAAATGTGAT GACATGATGA CGTGTTACCT GTTCCACATG 
TACGTGGGGG TCCGAGCTGG TGGGGGCATC GGGGATGAGA TTGAGGATCC AGCAGGGGAT 
GAATACGAGC TCTACCGTGT GGTCTTTGAC ATCACCTTTT TCTTCTTCGT CATCGTCATC 
CTGCTGGCCA TTATCCAAGG TCTGATCATT GATGCTTTTG GGGAGCTCCG AGACCAGCAA 
GAGCAAGTGA AGGAAGATAT GGAGACCAAA TGTTTCATCT GTGGCATTGG AAGTGACTAC 
TTTGATACAA CACCCCACGG TTTTGAGACC CACACGCTGG AGGAGCACAA CCTGGCTAAC 
TACATGTTTT TCCTGATGTA TCTGATCAAT AAGGATGAGA CCGAGCACAC GGGACAGGAG 
TCGTATGTCT GGAAGATGTA CCAGGAGCGC TGCTGGGACT TCTTCCCAGC TGGTGACTGT 
TTCCGAAAAC AGTATGAAGA CCAGCTGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012401108.1
CDS	1..13173
Translation	MGSLLEPICLKPSRLPAQEGKEEQQGYGEDEVVLQCSATTLKEQIKLCLAAEGFGNRLCFLEPTSNAQNVPPDLAVCCFVLEQSLSVRALQEMLANTVEAGTESSQGGGHRTLLYGHAILLRHAHSQMYLSCLTTSRSMTDKLAFDVGLQEDATGEACWWTMHPASKQRSEGEKVRVGDDLILVSVSSERYLHLSTASGELQADASFMQTLWNMNPICSGCEEGYVTGGHVLRLFHGHMDECLTISSADSDDQRRSGHEGRRAWMPGELGSGCRHCEMLGHLGDLGWDRAAGPDSKGLRDGMLTLVLNCIDRLNFYSTAAHFAEFAGEEAAESWKEIVNLLYELLASLIRGNRTNCALFSHNLDWLVSKLDRLEASSGILEVLYCVLIESPEVLNIIQENHIKSIISLLDKHGRNHKVLDVLCSLCVCNGVAVRSNQVLITENLLPGRELLLQTNLINYVTSIRPNIFVGRAEGTTQYGKWYFEVMVDSVEPFLTAQATHLRVGWALTEGYSPYPGGGEGWGGNGVGDDLYSYGFDGLHLWSGRIARPVASPGQHLLAAEDVVSCCLDLSVPSISFRINGCPVQGVFEAFNLDGLFFPVVSFSAGVRVRFLLGGRHGEFKFLPPPGYAPCHEALLPRERLRLEPIKEYRREGPHGPHLLGPTCCLSHTDFVPYPVDTVQIVLPPHLERIREKLAENIHELWALTRIEQGWTYGPVRDDNKRLHPCLVDFHSLPEPERNYNLQMSGETLKTLLALGCHVGMADEKAEENLKKTKLPKTYMMnullnullnullnullnullnullnullnullnullDLSHVRLTPAQTTLVDRLAENGHNVWARDRVGQGWSSAVQDIPARRNPRLVPYRLLDEATKRSNRDSLCQAVRTLLGYGYNIEPPDQESSQVESQTSGDRVRIFRAERSYAVQSGRWYFEFEAVTTGEMRVGWARPELRPDVELGADDLAYVFNGHRGQRWHMGSEPFGRPWQPGDVVGCMIDLSENNILFTLNGEVLMSDSGSETAFKDIEIGDGFLPVCSLGLGQVGRLNLGQDVGSLRYFTICGLQEGFEPFAINMQRPVTTWFSKSLPQFEPMPPDHPHYEVTRVDGTVDTPPCLRLTHRTWGSQNSLVEMMFLRLSLPVQFYQHFRCTPGATPLAPPGPQPPGDDEVRAAEPDPDYEQMQRSAGRTGEAEGNKEGTGGPTKDGAASQnullnullnullnullnullnullnullKDAATEKSKKRGFLFKAKKAAMMTQPPATPALPRLPHDVVSADNRDDPEIILNTTTYYYSVRVFAGQEPSSVWVGWVTPDYHQHDMNFDLTKVRVVTVTMGDERGNVHNSLKRSNCYMVWGGDFVSPGQQGRISHTDLVIGCLVDLATGLMTFTANGKESNTFFQVEPNTKLFPAAFVLPTNQNVMQFELGKLKNIMPLSAAMFLSERKNPAPQCPPRLEVQMLMPVSWSRMPNHFLQVETHRAGDRLGWAVQCEEPLTMMALHIPEENRGGHWPTMVGASTSTPRDLELVLQVMGIFGDEDVKQILRMIEPEVFKEEEEEEEEEEEEEDEEEKEEDEEEEEEEKEEEVKEEEEEEEEKGEEEEAVEEGLLQMKLPESVKLQMCHLLEYFCDQDLQHRVEALIAFSERYVSCLQNNQRSRYGLLIKAFTMSAAETARRTREFRSPPQEQINMLLHFKGSEDEEDCPVPDEIRQELLDFHQDLLLHCGIQLETEEEEVEEETTLGGRLMSFLEKVKLVKKKEKEPDETPPPEEKKPESLQELVSHTMVRWAQEDFIQNPELVRAMFSLLHRQYDGLGELLRALPRAYTISPSSVEDTMSLLESLCQIRSLLIVQMGPQEENLMIQSIGNIMNNKVFYQHPNLMRALGMHETVMEVMVNVLGGGESKEIRFPKMVTSCCRFLCYFCRISRQNQRSMFDHLSYLLENSGIGLGMQGSTPLDVAAASVIDNNELALALQEQDLEKVVSYLAGCGLQSCPMLLAKGYPDIGWNPCGGERYLDFLRFAVFVNGESVEENANVVVRLLIRRPECFGPALRGEGGSGLLAAMEEAIRISEDPARDGPGVRKDRRREHHGGEEAPEENRVHLGNAIMSFYSALIDLLGRCAPETHLIQAGKGEALRIRAILRSLVPLDDLVGIISLPLQIPTLGKDGALVQPRMSASFVPDHKAPMVLFLDRVYGIDSQDFLLHVLDVGFLPDMRAAASLDTATFSTTEMALALNRYLCTAVLPLITKCAPLFAGTEHRAIMVDSMLHTVYRLSRGRSLTKAQRDVIEDCLMALCRYIRPSMLQHLLRRLVFDVPILNEFAKMPLKLLTNHYERCWKYYCLPTGWANYGVTSEEELHLTRKLFWGIFESLAHKKYDQELYRMAMPCLCAIAGALPPDYVDASYSSKAEKKATVDAEGNFDPRPVETLNVIIPEKLDSFINKFAEYTHEKWAFDKIQNNWSFGENIDEELKTHHMLRPYKTFSEKDKEIYRWPIKESLKAMIAWEWTVEKAREGEEEKGEKKTKTRKISQNTPATYDPREGYNPQPPDLTCVTLSRELQAMAEQLAENYHNTWGRKKKQELEAKGGGTHPLLVPYDTLTAKEKARDREKAQELLKFLQMNGYAVTRCPQAFLHLPRALSCSPRTVMKSGPEIVKAGLRSFFESASEDIEKMVENLRLGKVSQARTQVKGVGQNLTYTTVALLPVLTTLFQHIAQHQFGDDVILDDVQVSCYRTLCSIYSLGTTKNSYVEKLRPALGECLARLAAAMPVAFLEPQLNEFNACSVYTTKSPRERAILGLPSSVEEMCPDIPDLKHLIAEIGGRAESGARYTEMPHVIEITLPMLCNYLPRWTVNDTQAHWLTEPNPDAEELFRMIAEIFIYWSKSHNFKREEQNFVVQNEINNMSFLTADSKSKMAKSGGSDQERTKKKRRGDRYSVQTSLIVATLKKMLPIGLNMCAPTDQDLIALAKTRYALKDTDEEVREFLQNNLHLQGKSEGSPSLRWQMALYRGLPGRAEDADAPENIVRRVQEVSAVLYHLEQTEHPYKSKKAVWHKLLSKQRRRAVVACFRMTPLYNLPRHRASNMFLESYKASWILTEEHSFEDRMIDDLSKAGEEEEEEEEQEEKKPDPLHQLVLHFSRTALTEKSKLDEDYLYMAYADIMAKSCHLEEEEEPSEPAEEEAEVSFEEKEMEKQKLLYQQSRLHNRGAAEMVLQMISACKGEPGVMVSSTLKLGISILNGGNMEVQQKMLDYLKDKKDVGFFQSIQALMQTCRSVLCSAGQAGLTEGWRNGETG*PSLSVPGEKVMADDEFTQDLFRFLQLLCEGHNNDFQNYLRTQTGNTTTINIIICTVDYLLRLQESISDFYWYYSGKDVIEEQGKRNFSKAMAVAKQVFNSLTEYIQDSSQIELLKELLDLQKDMVVMLLSLLEGNVVNGTIARQMVDMLVESSSNVEMILKFFDMFLKLKDIVGSEAFQDYVTDPRGLISKKDFQKAMDSQKQFTGPEIQFLLSCSEADENEMINVEEFASRFQEPARDIGFNVAVLLTNLSEHVPHDPRLKNFLELAESILEYFRPYLGRIEIMGASRRIERIYFEISETNKAQWEMPQVKESKRQFIFDVVNEGGESEKMEMFVSFCEDTIFEMQIAAQISEPEGEPEEDEEESMAESGPDSGEAGPDGEGAGAGGPTGISGHVAGAVSSVLRKLTYRSLRRRVRRLRKLTLREAAGTGAGLIWAGATWTGSGVIGATRGALGLLWGSLFGGGLVEGAKKVTVTGLLAGMPDPTGDEVHGEQAAGAGAAGTGDGAVEGDEEVGAELAEREAEAEAGAGEAGPAGGEGAGANGGHFRMEVPGGLGDMGDTTPVEPPTPEGSPIFRRKLGLEGEEEQALEPEPEPEPEPEPEPEPEPEKADAENGEKEGSQEPEPEAPKKAAAPPPPRKEDAKSEGPDFWEEMEVQRVKFLNYLSRNFYTLRFLALFLAFAINFILLFYKVSDSPPGEEDLEGSGLEGLAGSGSGDFLGSGMGSGAGEDLENEEEENMVYYFLEESTGYMEPALRGLSILHTLIAFLCIIGYNCLKVPLVIFKREKELARKLEFDGLYITEQPEDDDVKGQWDRLVLNTPSFPSNYWDKFVKRKVLEKHGDIFGRERIAELLGMDLASLEITAQNEKRPEPPSGLLTWIMSIDVKYQIWKFGVIFTDNSFLYLTWYMVMSLLGHYNNFFFAAHLLDIAMGVKTLRTILSSVTHNGKQLVMTVGLLAVVVYLYTVVAFNFFRKFYNKSEDEDEPDMKCDDMMTCYLFHMYVGVRAGGGIGDEIEDPAGDEYELYRVVFDITFFFFVIVILLAIIQGLIIDAFGELRDQQEQVKEDMETKCFICGIGSDYFDTTPHGFETHTLEEHNLANYMFFLMYLINKDETEHTGQESYVWKMYQERCWDFFPAGDCFRKQYEDQLA

Target ORF information:

RefSeq Version	XM_012545654.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ryanodine receptor 1 (skeletal) (RYR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012545654.1

ORF Insert Sequence:

ATGGGGTCCC TGTTAGAACC CATCTGTCTA AAACCGTCTC GGCTCCCGGC CCAAGAAGGG 
AAAGAGGAGC AGCAGGGCTA CGGGGAAGAT GAGGTTGTCC TGCAGTGCAG CGCTACCACC 
CTCAAGGAGC AAATCAAGCT CTGCCTAGCC GCCGAAGGCT TTGGAAACCG ACTCTGTTTT 
TTGGAACCTA CCAGCAATGC CCAGAATGTG CCCCCTGACT TGGCTGTGTG CTGCTTTGTG 
CTGGAACAAT CTCTCTCGGT GCGAGCCCTG CAGGAGATGC TGGCCAACAC AGTGGAGGCT 
GGCACTGAGT CATCTCAGGG CGGGGGACAC CGGACCCTTC TCTATGGCCA CGCCATCTTA 
CTCCGGCATG CCCACAGCCA AATGTACCTA AGCTGCCTCA CAACATCCCG CTCTATGACT 
GACAAACTCG CCTTCGATGT GGGCCTGCAA GAAGATGCCA CAGGGGAGGC CTGCTGGTGG 
ACCATGCATC CGGCCTCAAA GCAGCGATCC GAAGGAGAGA AGGTCCGGGT GGGGGATGAC 
CTCATCCTTG TCAGTGTCTC TTCTGAAAGA TACTTGCACC TGTCCACAGC CAGTGGGGAG 
CTCCAGGCTG ATGCTTCTTT CATGCAAACC CTTTGGAACA TGAACCCCAT CTGTTCTGGG 
TGTGAGGAAG GGTACGTGAC CGGTGGCCAT GTTCTCCGGC TGTTCCACGG CCACATGGAC 
GAGTGTCTAA CCATCTCTTC AGCTGACAGT GATGACCAGC GCAGGTCTGG CCATGAGGGG 
CGGAGGGCCT GGATGCCAGG GGAATTAGGG AGTGGATGTC GTCACTGTGA GATGCTTGGG 
CATCTAGGTG ATTTAGGGTG GGACAGGGCT GCGGGCCCAG ACTCTAAGGG GTTGAGGGAT 
GGAATGCTGA CTTTGGTCCT GAATTGTATC GATCGGCTCA ACTTCTACTC CACGGCTGCC 
CACTTTGCTG AGTTTGCAGG AGAGGAGGCA GCTGAGTCTT GGAAAGAGAT TGTGAACTTA 
CTCTATGAGC TTTTGGCTTC TTTGATTCGG GGAAACCGTA CCAATTGTGC TCTCTTCTCC 
CACAACCTCG ACTGGCTTGT CAGCAAGTTG GATCGGCTCG AGGCCTCTTC TGGAATCCTG 
GAGGTCCTTT ATTGTGTACT GATTGAGAGT CCAGAAGTAC TGAACATTAT TCAGGAGAAT 
CACATCAAGT CCATCATTTC CCTGTTGGAC AAGCATGGCC GGAACCACAA GGTTCTGGAC 
GTGTTGTGCT CTCTGTGCGT GTGTAATGGC GTTGCTGTCC GCTCCAACCA GGTCCTGATC 
ACCGAGAACC TCCTTCCCGG GCGTGAGCTC CTACTACAGA CCAACCTCAT TAACTATGTC 
ACCAGCATAC GCCCTAATAT CTTCGTGGGC CGGGCTGAGG GGACCACACA GTATGGGAAG 
TGGTACTTTG AAGTCATGGT AGACTCTGTG GAACCTTTCC TGACTGCTCA AGCCACACAC 
CTGAGAGTGG GCTGGGCCCT CACTGAGGGA TACAGCCCCT ACCCTGGTGG TGGGGAAGGC 
TGGGGTGGCA ATGGCGTTGG AGATGACCTC TACTCCTATG GCTTTGATGG ACTGCACCTC 
TGGTCTGGGA GGATTGCCCG GCCAGTGGCC TCTCCAGGCC AGCACCTCCT GGCTGCCGAG 
GATGTAGTTA GCTGCTGTCT GGATCTGAGT GTCCCATCCA TCTCATTCCG AATCAATGGC 
TGCCCCGTGC AAGGCGTCTT TGAGGCTTTC AACCTGGATG GGCTCTTCTT CCCAGTGGTC 
AGCTTCTCTG CTGGTGTTCG GGTACGGTTT CTCCTGGGTG GTCGCCATGG AGAGTTCAAA 
TTCCTCCCCC CACCGGGCTA TGCCCCATGC CATGAAGCTC TACTACCCCG TGAGCGCCTC 
CGGCTGGAGC CCATTAAGGA GTACCGTCGA GAAGGACCCC ATGGGCCTCA CCTCTTGGGA 
CCTACTTGCT GCTTGTCCCA CACCGACTTT GTGCCCTACC CTGTGGACAC TGTCCAGATT 
GTTTTGCCAC CTCATCTGGA ACGGATCCGA GAGAAACTGG CAGAGAATAT CCATGAGCTC 
TGGGCCCTGA CTCGCATCGA GCAAGGATGG ACCTATGGCC CGGTACGGGA TGATAACAAG 
CGGCTTCACC CATGCCTGGT GGACTTTCAC AGCCTCCCAG AGCCTGAGAG AAACTACAAT 
CTGCAGATGT CAGGAGAGAC ACTCAAGACC CTGCTGGCTT TGGGATGTCA TGTAGGCATG 
GCTGATGAGA AGGCTGAGGA AAACCTCAAG AAGACAAAAC TCCCCAAGAC GTACATGATG 
AGNNNNNNNN NNNNNNNNNN NNNNNTGGAC CTGAGCCACG TGCGGTTAAC CCCAGCACAG 
ACGACGCTCG TTGACCGGCT GGCTGAGAAT GGGCACAATG TGTGGGCGAG AGACCGTGTG 
GGGCAAGGCT GGAGTTAAAG TGCTGTCCAA GACATCCCTG CCCGGAGGAA CCCCCGGCTT 
GTCCCCTACC GCCTCCTGGA TGAGGCTACC AAGCGCAGCA ATCGAGACAG CCTCTGCCAA 
GCAGTGCGCA CCCTCTTGGG TTATGGCTAT AACATTGAGC CACCTGACCA GGAATCCAGC 
CAAGTGGAGA GCCAGACCAG TGGGGACCGA GTACGCATCT TCCGAGCAGA ACGCAGCTAC 
GCTGTTCAGA GCGGTCGCTG GTATTTTGAG TTTGAGGCCG TGACCACTGG AGAAATGCGT 
GTGGGCTGGG CCCGTCCAGA GCTGCGGCCA GACGTGGAGC TGGGAGCAGA TGATTTGGCT 
TATGTCTTCA ATGGCCACCG AGGGCAGCGC TGGCACATGG GCAGTGAGCC ATTTGGACGA 
CCCTGGCAAC CAGGCGACGT GGTTGGGTGT ATGATCGACC TTTCGGAGAA CAACATCCTC 
TTCACACTCA ATGGAGAAGT GCTCATGTCT GACTCGGGCT CGGAGACGGC CTTTAAGGAT 
ATTGAGATTG GGGATGGCTT CCTCCCTGTC TGCAGCCTGG GCCTGGGCCA GGTGGGACGC 
CTAAATTTGG GCCAGGATGT GGGATCTCTT CGCTATTTCA CCATCTGTGG ACTTCAGGAG 
GGCTTTGAAC CTTTTGCCAT CAACATGCAG CGGCCTGTCA CCACCTGGTT CAGCAAGAGC 
CTGCCCCAGT TTGAACCGAT GCCCCCTGAC CATCCCCACT ATGAGGTGAC CCGAGTGGAC 
GGCACGGTGG ACACGCCTCC CTGCCTGCGC CTGACGCACC GTACCTGGGG CTCCCAGAAC 
AGCCTGGTGG AGATGATGTT CCTGAGGCTG AGCCTCCCGG TGCAGTTCTA TCAGCACTTC 
CGCTGCACCC CGGGGGCCAC CCCCTTGGCC CCGCCGGGTC CCCAGCCCCC CGGGGACGAC 
GAGGTCCGAG CGGCCGAGCC TGACCCCGAC TACGAGCAGA TGCAGCGTTC GGCGGGCCGG 
ACGGGCGAGG CTGAGGGGAA CAAGGAGGGG ACTGGGGGGC CCACCAAGGA CGGGGCGGCT 
TCTCAGNNNN NNNNNNNNNN NNNNNNGAAG GACGCTGCCA CTGAGAAAAG CAAGAAGAGG 
GGGTTCTTGT TCAAAGCCAA GAAGGCAGCC ATGATGACGC AACCACCCGC CACCCCCGCT 
CTGCCCCGAC TCCCTCACGA CGTGGTGTCC GCGGACAACA GGGATGACCC TGAGATTATC 
CTCAACACCA CCACGTATTA CTACTCTGTC CGGGTGTTTG CCGGCCAGGA GCCCAGCTCA 
GTGTGGGTGG GCTGGGTCAC CCCTGACTAC CACCAGCACG ACATGAATTT TGATCTGACC 
AAAGTCCGCG TTGTGACGGT GACCATGGGG GACGAGCGAG GCAATGTACA CAACAGCTTG 
AAGCGCAGTA ACTGCTACAT GGTGTGGGGT GGGGATTTTG TGAGCCCGGG ACAGCAAGGA 
CGCATCAGTC ACACAGACCT GGTCATCGGC TGCCTGGTGG ACCTGGCCAC CGGCCTCATG 
ACCTTCACGG CCAATGGAAA AGAGAGCAAC ACCTTTTTCC AGGTGGAGCC AAACACGAAG 
CTGTTTCCAG CGGCCTTCGT CCTTCCCACC AACCAAAATG TTATGCAATT TGAACTGGGA 
AAGCTGAAGA ACATCATGCC CCTGTCAGCA GCCATGTTCC TGAGTGAACG GAAGAACCCA 
GCGCCGCAGT GTCCCCCCCG CTTGGAGGTA CAAATGCTCA TGCCCGTGTC CTGGAGCAGG 
ATGCCCAACC ACTTCCTACA AGTGGAGACA CACCGGGCAG GTGACCGCCT GGGCTGGGCT 
GTGCAGTGTG AGGAGCCTCT CACCATGATG GCCTTGCATA TCCCTGAGGA GAACCGGGGA 
GGCCATTGGC CTACGATGGT GGGAGCCTCG ACTTCCACAC CGAGGGACCT GGAACTGGTC 
CTGCAGGTGA TGGGCATCTT CGGGGACGAG GACGTCAAGC AAATCCTGAG GATGATTGAG 
CCTGAAGTTT TCAAGGAAGA GGAAGAGGAG GAAGAGGAGG AGGAGGAGGA GGAGGATGAA 
GAAGAGAAGG AAGAGGATGA AGAAGAAGAG GAAGAAGAAA AAGAGGAGGA GGTGAAAGAG 
GAGGAGGAGG AAGAGGAAGA GAAGGGAGAG GAAGAAGAAG CTGTGGAGGA AGGACTACTT 
CAGATGAAGC TGCCTGAGTC TGTCAAGCTG CAGATGTGCC ATCTGCTGGA GTACTTCTGT 
GACCAGGATC TGCAGCACCG AGTGGAGGCG CTCATTGCGT TCTCGGAGCG CTACGTCAGT 
TGCCTGCAGA ACAACCAGCG CAGCCGCTAC GGGCTGCTCA TAAAGGCCTT CACTATGTCC 
GCAGCGGAGA CTGCCCGGCG CACCCGGGAG TTTCGCTCTC CGCCCCAGGA GCAGATCAAC 
ATGCTTCTGC ACTTCAAAGG GAGTGAAGAC GAGGAGGACT GTCCGGTCCC AGACGAAATT 
CGGCAGGAGC TGCTGGATTT TCACCAGGAC CTGCTGCTGC ACTGCGGCAT CCAGCTGGAA 
ACGGAGGAGG AGGAGGTGGA AGAGGAGACC ACCCTGGGAG GCCGGCTCAT GAGCTTTTTG 
GAGAAGGTGA AGCTGGTGAA GAAGAAGGAG AAGGAGCCAG ATGAGACCCC TCCCCCAGAA 
GAGAAAAAGC CTGAGTCCTT GCAGGAGCTG GTCTCTCACA CAATGGTGCG CTGGGCCCAA 
GAGGACTTTA TCCAGAACCC AGAGCTGGTC CGGGCCATGT TCAGCCTCCT CCACCGGCAG 
TACGATGGCC TCGGGGAGCT GCTCCGTGCC CTGCCCAGGG CTTACACCAT CTCCCCGTCC 
TCCGTGGAGG ACACCATGAG CCTCCTGGAA AGCCTGTGTC AGATCCGCTC CTTGCTCATT 
GTGCAGATGG GGCCGCAAGA GGAGAATCTC ATGATCCAGA GCATTGGGAA TATCATGAAC 
AACAAGGTGT TTTACCAGCA CCCAAATCTG ATGAGGGCTT TGGGGATGCA CGAAACAGTG 
ATGGAAGTTA TGGTCAATGT GTTAGGAGGG GGAGAGTCTA AGGAAATCCG CTTCCCCAAA 
ATGGTCACCA GCTGCTGCCG GTTCCTCTGC TATTTCTGTC GCATTAGTCG CCAGAACCAA 
AGGTCCATGT TTGATCACCT CAGCTACCTG CTGGAGAACA GCGGGATCGG CCTGGGTATG 
CAGGGTTCCA CTCCGCTAGA TGTGGCGGCT GCCTCCGTCA TAGACAACAA CGAGCTGGCC 
TTGGCCCTTC AGGAACAAGA CCTGGAAAAG GTCGTCTCCT ACTTGGCCGG CTGCGGCCTT 
CAGAGCTGCC CCATGCTGCT GGCCAAGGGC TACCCCGACA TTGGCTGGAA CCCCTGCGGG 
GGGGAGCGCT ATCTGGATTT CCTGAGATTT GCCGTCTTTG TTAATGGAGA GAGCGTGGAA 
GAGAATGCGA ATGTTGTGGT TCGTCTGCTC ATCCGGCGTC CCGAATGCTT CGGCCCGGCC 
CTGCGAGGAG AGGGCGGCTC TGGGCTGCTG GCTGCTATGG AAGAGGCAAT CCGGATCTCA 
GAGGATCCTG CTCGAGATGG CCCGGGCGTC AGGAAGGACC GGAGGCGGGA GCACCACGGG 
GGTGAAGAGG CCCCTGAAGA AAACCGTGTG CACTTGGGAA ATGCCATCAT GTCATTCTAT 
TCCGCCCTGA TCGACCTGCT GGGACGCTGT GCTCCAGAGA CTCATCTGAT CCAGGCCGGG 
AAGGGAGAAG CGCTGAGGAT CCGAGCCATT CTGCGCTCCC TGGTGCCCCT GGACGACCTC 
GTGGGCATTA TCAGCCTGCC CCTGCAGATC CCCACCCTGG GCAAAGATGG GGCCCTGGTG 
CAGCCTCGGA TGTCTGCCTC CTTCGTGCCT GACCACAAAG CCCCCATGGT CCTCTTCCTG 
GACCGCGTCT ACGGCATCGA CAGCCAGGAC TTCCTGCTCC ACGTGCTTGA TGTCGGCTTC 
CTGCCTGACA TGCGGGCGGC TGCCTCCCTG GACACGGCAA CCTTCAGCAC CACGGAGATG 
GCCCTGGCCT TGAACCGCTA CCTGTGCACA GCCGTGCTTC CCCTGATCAC CAAGTGCGCC 
CCACTGTTTG CGGGGACGGA GCACCGCGCC ATCATGGTGG ACTCCATGCT CCACACGGTG 
TACCGCCTGT CCCGGGGACG CTCTCTCACC AAGGCCCAGC GAGATGTCAT TGAGGACTGC 
CTCATGGCGC TCTGCAGGTA CATCCGTCCA TCTATGCTCC AGCACCTTCT GCGTCGTCTG 
GTATTCGACG TTCCCATCCT CAACGAGTTT GCCAAGATGC CCCTCAAGCT CCTGACCAAT 
CACTATGAGC GCTGCTGGAA ATATTACTGC CTCCCTACGG GCTGGGCCAA CTATGGAGTT 
ACCTCCGAGG AAGAGCTGCA TCTGACTCGC AAGCTTTTCT GGGGCATCTT TGAGTCACTG 
GCCCACAAGA AATACGACCA GGAGCTCTAC CGCATGGCCA TGCCGTGTCT GTGCGCCATC 
GCCGGGGCCC TGCCCCCCGA CTACGTGGAT GCCTCCTACT CCTCCAAAGC TGAGAAGAAG 
GCCACTGTGG ATGCTGAGGG CAACTTTGAC CCCCGGCCCG TGGAGACACT CAATGTGATC 
ATACCCGAGA AACTCGACTC CTTCATCAAC AAATTTGCCG AGTACACACA CGAGAAGTGG 
GCATTTGACA AGATTCAGAA CAATTGGTCA TTTGGGGAGA ACATTGACGA GGAGCTCAAG 
ACCCACCACA TGCTTCGGCC CTACAAGACC TTCTCAGAAA AGGACAAGGA GATTTACCGA 
TGGCCCATCA AGGAGTCCCT GAAGGCCATG ATCGCCTGGG AGTGGACTGT GGAAAAGGCC 
CGTGAGGGTG AGGAGGAGAA GGGAGAGAAG AAGACCAAGA CTCGCAAGAT CTCCCAGAAT 
ACCCCGGCCA CCTATGACCC AAGAGAGGGC TATAATCCCC AGCCTCCAGA CCTTACCTGT 
GTCACCCTGT CCCGGGAACT GCAGGCCATG GCTGAACAAC TGGCTGAGAA TTACCATAAC 
ACATGGGGGA GGAAGAAAAA GCAGGAATTA GAAGCCAAGG GTGGAGGCAC CCACCCCCTC 
CTGGTCCCCT ATGACACACT GACGGCCAAG GAGAAGGCTC GCGATAGAGA GAAAGCCCAG 
GAACTGCTCA AATTCCTGCA GATGAACGGC TACGCGGTGA CGCGGTGCCC CCAGGCCTTC 
CTCCATCTCC CCCGAGCCCT GTCCTGTTCC CCCAGGACCG TGATGAAGTC TGGTCCCGAG 
ATCGTCAAGG CTGGTCTCCG ATCGTTCTTC GAGAGCGCTT CGGAGGACAT CGAGAAGATG 
GTGGAGAACC TGCGCCTGGG CAAGGTGTCC CAGGCGCGGA CGCAGGTCAA GGGCGTCGGC 
CAGAACCTCA CCTACACCAC CGTGGCTCTG CTTCCCGTTC TCACCACGCT TTTCCAGCAC 
ATCGCCCAGC ACCAGTTTGG GGATGATGTC ATCTTGGACG ACGTCCAGGT CTCCTGCTAC 
CGAACACTTT GCAGCATCTA CTCTCTGGGG ACCACCAAGA ATTCCTATGT GGAGAAGCTC 
CGTCCAGCCC TTGGAGAATG CCTTGCCCGT CTGGCAGCTG CTATGCCAGT TGCCTTCCTT 
GAACCCCAAC TGAATGAGTT CAACGCCTGC TCTGTCTACA CCACCAAATC TCCCCGAGAA 
CGTGCCATCC TGGGCCTTCC GAGCTCCGTG GAGGAGATGT GCCCAGATAT CCCAGACCTG 
AAGCACCTGA TTGCCGAGAT CGGGGGGCGG GCCGAGTCGG GCGCCCGTTA CACCGAGATG 
CCCCATGTCA TCGAGATCAC GCTGCCGATG CTCTGTAACT ACCTGCCCCG CTGGACAGTG 
AATGACACAC AAGCCCACTG GCTAACAGAA CCCAACCCTG ATGCAGAAGA ACTCTTCAGG 
ATGATAGCAG AAATCTTCAT CTATTGGTCC AAGTCTCATA ACTTCAAGAG AGAAGAACAG 
AATTTCGTGG TACAGAACGA AATCAATAAC ATGTCCTTCC TGACGGCAGA TAGCAAGAGC 
AAGATGGCCA AGTCAGGAGG CTCCGATCAA GAGAGAACAA AGAAGAAACG GCGAGGGGAC 
CGATATTCCG TCCAGACCTC TCTGATCGTG GCTACCCTCA AGAAGATGCT GCCTATTGGG 
TTGAACATGT GTGCACCCAC TGACCAGGAC CTCATCGCGC TGGCCAAGAC TCGTTATGCC 
CTGAAAGACA CAGATGAAGA AGTTCGGGAA TTTCTGCAGA ATAACCTTCA TCTTCAGGGA 
AAGAGTGAGG GCTCCCCATC ACTGCGCTGG CAGATGGCCC TGTACCGGGG GCTGCCTGGC 
CGGGCTGAGG ATGCTGATGC CCCTGAGAAC ATTGTTCGCC GAGTACAGGA AGTCTCAGCG 
GTGCTTTACC ACCTGGAACA GACAGAACAT CCCTACAAGT CCAAGAAAGC TGTGTGGCAC 
AAGCTGCTTT CAAAACAGCG TCGTCGGGCG GTTGTGGCTT GTTTTCGCAT GACACCCTTG 
TACAACTTGC CTCGGCATCG GGCAAGCAAT ATGTTCTTGG AGTCCTACAA AGCATCATGG 
ATCCTAACTG AGGAGCACAG TTTTGAGGAC CGCATGATTG ATGACCTTTC TAAAGCTGGG 
GAGGAAGAAG AAGAGGAAGA AGAACAGGAA GAGAAGAAGC CAGACCCTCT TCACCAGCTG 
GTATTACACT TTAGCCGGAC AGCCCTGACA GAGAAGAGCA AACTAGATGA GGACTATTTG 
TACATGGCAT ATGCTGACAT CATGGCCAAG AGCTGCCACT TGGAGGAGGA AGAGGAGCCT 
TCAGAACCTG CTGAGGAGGA GGCTGAAGTT TCATTTGAGG AGAAGGAGAT GGAAAAGCAG 
AAACTGCTCT ACCAGCAGTC AAGGCTACAC AACCGAGGGG CTGCTGAGAT GGTGCTACAA 
ATGATTAGTG CCTGCAAAGG GGAGCCGGGA GTCATGGTTT CATCCACCCT GAAGTTGGGC 
ATCTCCATCC TCAACGGTGG CAACATGGAG GTGCAGCAGA AGATGCTGGA CTATCTGAAG 
GACAAAAAGG ATGTTGGCTT CTTCCAGAGC ATCCAGGCCC TGATGCAGAC ATGCAGGTCT 
GTCCTGTGCA GTGCAGGGTG ACAGGCTGGA TTGACAGAGG GATGGAGAAA TGGAGAGACA 
GGCTGACCCT CCCTCTCTGT CCCAGGGGAA AAAGTAATGG CCGACGATGA GTTCACCCAG 
GATCTCTTCC GCTTTCTGCA GCTGCTGTGT GAAGGCCATA ACAATGACTT TCAGAACTAC 
CTCCGCACGC AGACGGGGAA CACGACCACC ATCAACATCA TCATCTGCAC AGTGGATTAT 
CTGCTGAGGC TGCAGGAGTC CATCAGTGAC TTCTACTGGT ACTACTCGGG GAAGGATGTG 
ATCGAAGAGC AAGGGAAGAG GAACTTCTCC AAAGCCATGG CCGTGGCCAA GCAGGTCTTC 
AACAGCCTCA CCGAGTACAT CCAGGACTCA AGCCAGATTG AGCTGCTGAA GGAGCTCCTG 
GATCTGCAGA AAGACATGGT GGTCATGTTG CTTTCGCTGC TGGAAGGTAA CGTGGTCAAC 
GGCACAATTG CCCGGCAGAT GGTGGACATG CTGGTGGAGT CCTCCTCCAA CGTGGAGATG 
ATCCTCAAGT TCTTCGACAT GTTCCTAAAG CTGAAGGACA TCGTGGGCTC CGAGGCATTC 
CAGGACTACG TCACCGACCC CCGTGGACTC ATCTCCAAGA AGGATTTCCA GAAGGCCATG 
GACAGCCAGA AGCAGTTCAC TGGCCCAGAG ATCCAGTTCC TCCTGTCCTG CTCAGAGGCG 
GATGAGAACG AGATGATCAA TGTGGAGGAA TTTGCCAGCC GCTTCCAGGA GCCGGCCCGA 
GACATCGGCT TCAACGTGGC CGTGCTGCTG ACCAACCTGT CGGAGCACGT GCCCCACGAC 
CCGCGCCTCA AGAACTTCCT GGAGCTGGCT GAGAGCATCC TGGAGTACTT CCGGCCCTAC 
CTGGGCCGGA TCGAGATCAT GGGGGCCTCC CGGCGCATCG AGCGCATCTA CTTTGAAATC 
TCTGAGACCA ACAAAGCACA GTGGGAGATG CCCCAGGTGA AGGAGTCCAA ACGCCAGTTC 
ATCTTTGACG TGGTGAATGA AGGGGGGGAG TCGGAGAAGA TGGAAATGTT TGTGAGCTTC 
TGTGAAGACA CCATCTTTGA GATGCAGATC GCCGCCCAGA TCTCAGAGCC CGAGGGAGAG 
CCTGAGGAAG ATGAGGAGGA GTCCATGGCA GAGTCGGGGC CAGACTCCGG GGAGGCAGGG 
CCCGACGGAG AAGGGGCCGG CGCAGGGGGC CCGACGGGCA TCTCCGGGCA CGTGGCCGGT 
GCCGTCTCCT CGGTCCTGCG GAAGCTGACC TACCGGAGCC TGCGCAGGAG GGTGCGGAGG 
CTCCGCAAGC TGACCCTGCG GGAGGCTGCA GGAACCGGGG CCGGGCTCAT CTGGGCCGGG 
GCCACGTGGA CGGGCTCCGG AGTGATCGGC GCCACCCGGG GGGCCCTGGG CCTACTCTGG 
GGCTCTCTGT TTGGGGGAGG GCTGGTGGAG GGAGCCAAGA AAGTGACCGT GACGGGACTG 
TTGGCCGGGA TGCCAGACCC CACAGGCGAT GAGGTCCATG GGGAGCAGGC GGCTGGCGCC 
GGGGCTGCCG GGACTGGGGA CGGAGCCGTG GAGGGTGATG AGGAGGTGGG GGCGGAGTTG 
GCCGAGAGGG AGGCTGAGGC TGAGGCCGGA GCTGGGGAGG CCGGGCCAGC CGGAGGAGAG 
GGAGCCGGGG CCAATGGAGG CCACTTCCGG ATGGAGGTCC CCGGAGGCCT GGGTGACATG 
GGAGACACAA CACCCGTTGA GCCGCCCACT CCTGAGGGTT CCCCCATCTT CCGGAGGAAA 
CTAGGGCTGG AGGGAGAAGA AGAACAAGCC TTAGAACCAG AACCAGAGCC AGAACCAGAA 
CCAGAGCCAG AGCCAGAACC CGAGCCTGAG AAAGCTGATG CTGAGAACGG AGAAAAGGAA 
GGGTCCCAAG AGCCAGAACC TGAGGCTCCC AAGAAGGCAG CTGCCCCACC TCCGCCCCGG 
AAGGAGGACG CCAAATCTGA GGGCCCTGAC TTCTGGGAGG AAATGGAGGT GCAGAGGGTC 
AAGTTCCTGA ACTACCTCTC CAGAAACTTC TATACCCTGA GATTCCTCGC CCTCTTCCTG 
GCCTTTGCCA TCAACTTCAT CCTGCTCTTC TACAAGGTGT CTGACTCTCC GCCAGGAGAA 
GAGGACCTGG AAGGCTCCGG TCTCGAGGGT CTGGCAGGCT CAGGGTCCGG GGACTTCCTG 
GGATCCGGCA TGGGCTCCGG GGCTGGGGAG GATCTGGAGA ACGAGGAGGA AGAGAACATG 
GTCTACTACT TCCTGGAAGA GAGTACGGGC TACATGGAGC CAGCCCTGCG AGGTCTCAGC 
ATCCTGCACA CCCTCATTGC CTTCCTCTGC ATCATAGGCT ACAACTGTCT CAAGGTACCG 
CTGGTTATAT TTAAACGGGA GAAGGAGCTA GCCCGGAAAC TAGAGTTCGA TGGCCTTTAC 
ATCACTGAGC AGCCCGAGGA TGACGACGTG AAGGGCCAGT GGGATCGACT GGTGCTTAAC 
ACCCCGTCCT TCCCCAGCAA CTACTGGGAC AAGTTCGTGA AAAGGAAGGT GCTGGAGAAA 
CATGGCGACA TCTTTGGCCG GGAGCGGATT GCAGAGCTGT TGGGCATGGA TCTGGCCAGC 
CTGGAGATCA CGGCGCAGAA CGAGAAGCGA CCGGAACCCC CCTCGGGACT GCTCACCTGG 
ATCATGTCCA TAGATGTCAA ATACCAAATC TGGAAATTTG GTGTCATCTT TACAGATAAT 
TCATTCTTGT ATTTGACGTG GTACATGGTC ATGTCCCTCC TCGGCCACTA TAACAACTTC 
TTCTTTGCCG CTCACCTGCT GGACATCGCC ATGGGAGTCA AGACGCTTCG GACCATCCTG 
TCTTCTGTCA CCCACAATGG GAAGCAGCTG GTGATGACGG TGGGCCTGCT GGCCGTCGTG 
GTTTACCTCT ACACGGTTGT GGCCTTCAAT TTCTTCCGCA AATTTTACAA TAAAAGCGAG 
GATGAGGATG AGCCGGACAT GAAATGTGAT GACATGATGA CGTGTTACCT GTTCCACATG 
TACGTGGGGG TCCGAGCTGG TGGGGGCATC GGGGATGAGA TTGAGGATCC AGCAGGGGAT 
GAATACGAGC TCTACCGTGT GGTCTTTGAC ATCACCTTTT TCTTCTTCGT CATCGTCATC 
CTGCTGGCCA TTATCCAAGG TCTGATCATT GATGCTTTTG GGGAGCTCCG AGACCAGCAA 
GAGCAAGTGA AGGAAGATAT GGAGACCAAA TGTTTCATCT GTGGCATTGG AAGTGACTAC 
TTTGATACAA CACCCCACGG TTTTGAGACC CACACGCTGG AGGAGCACAA CCTGGCTAAC 
TACATGTTTT TCCTGATGTA TCTGATCAAT AAGGATGAGA CCGAGCACAC GGGACAGGAG 
TCGTATGTCT GGAAGATGTA CCAGGAGCGC TGCTGGGACT TCTTCCCAGC TGGTGACTGT 
TTCCGAAAAC AGTATGAAGA CCAGCTGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RYR1 ( XM_023499964.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023355732.1 Sarcophilus harrisii ryanodine receptor 1 (RYR1), mRNA.

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CloneID	OSa152378
Clone ID Related Accession (Same CDS sequence)	XM_023499964.1
Accession Version	XM_023499964.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 14217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	LOW QUALITY PROTEIN: ryanodine receptor 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831767.1) and transcript sequences (GEDN01120056.1, GEDN01120059.1, GEDN01120073.1, GEDN01146501.1, GEDN01146502.1 and GEDN01146504.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases internal stop codons :: corrected 1 genomic stop codon assembly gap :: added 1489 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGGGGATG GAGGGGAGGG CGAGGATGAC GTCCAGTTTC TCCGAACTGA AGATGAGGTT 
GTCCTGCAGT GCAGCGCTAC CACCCTCAAG GAGCAAATCA AGCTCTGCCT AGCCGCCGAA 
GGCTTTGGAA ACCGACTCTG TTTTTTGGAA CCTACCAGCA ATGCCCAGAA TGTGCCCCCT 
GACTTGGCTG TGTGCTGCTT TGTGCTGGAA CAATCTCTCT CGGTGCGAGC CCTGCAGGAG 
ATGCTGGCCA ACACAGTGGA GGCTGGCACT GAGTCATCTC AGGGCGGGGG ACACCGGACC 
CTTCTCTATG GCCACGCCAT CTTACTCCGG CATGCCCACA GCCAAATGTA CCTAAGCTGC 
CTCACAACAT CCCGCTCTAT GACTGACAAA CTCGCCTTCG ATGTGGGCCT GCAAGAAGAT 
GCCACAGGGG AGGCCTGCTG GTGGACCATG CATCCGGCCT CAAAGCAGCG ATCCGAAGGA 
GAGAAGGTCC GGGTGGGGGA TGACCTCATC CTTGTCAGTG TCTCTTCTGA AAGATACTTG 
CACCTGTCCA CAGCCAGTGG GGAGCTCCAG GCTGATGCTT CTTTCATGCA AACCCTTTGG 
AACATGAACC CCATCTGTTC TGGGTGTGAG GAAGGGTACG TGACCGGTGG CCATGTTCTC 
CGGCTGTTCC ACGGCCACAT GGACGAGTGT CTAACCATCT CTTCAGCTGA CAGTGATGAC 
CAGCGCAGGC TGGTTCATTA TGAAGGGGGT GCTGTGTGCA CCCACGCCCG TTCCCTGTGG 
AGACTGGAAC CCCTGAGGAT AAGCTGGAGC GGGAGCCACC TGCGCTGGGG CCAGCCCCTG 
CGTGTGCGAC ACGTGACTAC AGGGAGATAC TTGGCACTGA CAGAGGACCA AGGCCTGATA 
GTGGTAGATG CCTCGAAGGC TGCCACCAAG GCAACTGCTT TCTGTTTCCG AATTTCTAAG 
GAGAAACTGG ACACAGCCCC AAAGCGAGAT GTGGAAGGCA TGGGCCCTCC CGAGATCAAG 
TATGGCGAGT CTCTGTGCTT TGTCCAACAC GTGGCCTCTG GCCTATGGCT CACCTACGCG 
GCCCCTGACC CCAAAGCCTT GCGCCTGGGC CTGATGAAGA GGAAGGCCAT GCTGCATCAG 
GAAGGCCACA TGGATGACGC TCTGTCCCTC ACTCGGTGCC AGCTGGAGGA GTCCCAGGCG 
GCCCGGATGA TCTACAGCAC AGCCGGGCTG TACAACCAGT TCATCAAGGG TCTGGACAGT 
CTGAGTGGGA AGCCCAAGGG GGCTCCGGCA GCAGGGGTGA CCCTGCCCAT AGAGGGGGTC 
ATCCTGAGCC TGCAGGACCT TATTGGCTAC TTCGAGCCCC CATCGGAAGA GCTCCAGCAT 
GAGGAGAAAC AGAGCAAACT GCGGAGCTTG CGGAATCGCC AGAGCCTCTT CCAGGAAGAG 
GGAATGCTGA CTTTGGTCCT GAATTGTATC GATCGGCTCA ACTTCTACTC CACGGCTGCC 
CACTTTGCTG AGTTTGCAGG AGAGGAGGCA GCTGAGTCTT GGAAAGAGAT TGTGAACTTA 
CTCTATGAGC TTTTGGCTTC TTTGATTCGG GGAAACCGTA CCAATTGTGC TCTCTTCTCC 
CACAACCTCG ACTGGCTTGT CAGCAAGTTG GATCGGCTCG AGGCCTCTTC TGGAATCCTG 
GAGGTCCTTT ATTGTGTACT GATTGAGAGT CCAGAAGTAC TGAACATTAT TCAGGAGAAT 
CACATCAAGT CCATCATTTC CCTGTTGGAC AAGCATGGCC GGAACCACAA GGTTCTGGAC 
GTGTTGTGCT CTCTGTGCGT GTGTAATGGC GTTGCTGTCC GCTCCAACCA GGTCCTGATC 
ACCGAGAACC TCCTTCCCGG GCGTGAGCTC CTACTACAGA CCAACCTCAT TAACTATGTC 
ACCAGCATAC GCCCTAATAT CTTCGTGGGC CGGGCTGAGG GGACCACACA GTATGGGAAG 
TGGTACTTTG AAGTCATGGT AGACTCTGTG GAACCTTTCC TGACTGCTCA AGCCACACAC 
CTGAGAGTGG GCTGGGCCCT CACTGAGGGA TACAGCCCCT ACCCTGGTGG TGGGGAAGGC 
TGGGGTGGCA ATGGCGTTGG AGATGACCTC TACTCCTATG GCTTTGATGG ACTGCACCTC 
TGGTCTGGGA GGATTGCCCG GCCAGTGGCC TCTCCAGGCC AGCACCTCCT GGCTGCCGAG 
GATGTAGTTA GCTGCTGTCT GGATCTGAGT GTCCCATCCA TCTCATTCCG AATCAATGGC 
TGCCCCGTGC AAGGCGTCTT TGAGGCTTTC AACCTGGATG GGCTCTTCTT CCCAGTGGTC 
AGCTTCTCTG CTGGTGTTCG GGTACGGTTT CTCCTGGGTG GTCGCCATGG AGAGTTCAAA 
TTCCTCCCCC CACCGGGCTA TGCCCCATGC CATGAAGCTC TACTACCCCG TGAGCGCCTC 
CGGCTGGAGC CCATTAAGGA GTACCGTCGA GAAGGACCCC ATGGGCCTCA CCTCTTGGGA 
CCTACTTGCT GCTTGTCCCA CACCGACTTT GTGCCCTACC CTGTGGACAC TGTCCAGATT 
GTTTTGCCAC CTCATCTGGA ACGGATCCGA GAGAAACTGG CAGAGAATAT CCATGAGCTC 
TGGGCCCTGA CTCGCATCGA GCAAGGATGG ACCTATGGCC CGGTACGGGA TGATAACAAG 
CGGCTTCACC CATGCCTGGT GGACTTTCAC AGCCTCCCAG AGCCTGAGAG AAACTACAAT 
CTGCAGATGT CAGGAGAGAC ACTCAAGACC CTGCTGGCTT TGGGATGTCA TGTAGGCATG 
GCTGATGAGA AGGCTGAGGA AAACCTCAAG AAGACAAAAC TCCCCAAGAC GTACATGATG 
AGNNNNNNNN NNNNNNNNNN NNNNNTGGAC CTGAGCCACG TGCGGTTAAC CCCAGCACAG 
ACGACGCTCG TTGACCGGCT GGCTGAGAAT GGGCACAATG TGTGGGCGAG AGACCGTGTG 
GGGCAAGGCT GGAGTTAAAG TGCTGTCCAA GACATCCCTG CCCGGAGGAA CCCCCGGCTT 
GTCCCCTACC GCCTCCTGGA TGAGGCTACC AAGCGCAGCA ATCGAGACAG CCTCTGCCAA 
GCAGTGCGCA CCCTCTTGGG TTATGGCTAT AACATTGAGC CACCTGACCA GGAATCCAGC 
CAAGTGGAGA GCCAGACCAG TGGGGACCGA GTACGCATCT TCCGAGCAGA ACGCAGCTAC 
GCTGTTCAGA GCGGTCGCTG GTATTTTGAG TTTGAGGCCG TGACCACTGG AGAAATGCGT 
GTGGGCTGGG CCCGTCCAGA GCTGCGGCCA GACGTGGAGC TGGGAGCAGA TGATTTGGCT 
TATGTCTTCA ATGGCCACCG AGGGCAGCGC TGGCACATGG GCAGTGAGCC ATTTGGACGA 
CCCTGGCAAC CAGGCGACGT GGTTGGGTGT ATGATCGACC TTTCGGAGAA CAACATCCTC 
TTCACACTCA ATGGAGAAGT GCTCATGTCT GACTCGGGCT CGGAGACGGC CTTTAAGGAT 
ATTGAGATTG GGGATGGCTT CCTCCCTGTC TGCAGCCTGG GCCTGGGCCA GGTGGGACGC 
CTAAATTTGG GCCAGGATGT GGGATCTCTT CGCTATTTCA CCATCTGTGG ACTTCAGGAG 
GGCTTTGAAC CTTTTGCCAT CAACATGCAG CGGCCTGTCA CCACCTGGTT CAGCAAGAGC 
CTGCCCCAGT TTGAACCGAT GCCCCCTGAC CATCCCCACT ATGAGGTGAC CCGAGTGGAC 
GGCACGGTGG ACACGCCTCC CTGCCTGCGC CTGACGCACC GTACCTGGGG CTCCCAGAAC 
AGCCTGGTGG AGATGATGTT CCTGAGGCTG AGCCTCCCGG TGCAGTTCTA TCAGCACTTC 
CGCTGCACCC CGGGGGCCAC CCCCTTGGCC CCGCCGGGTC CCCAGCCCCC CGGGGACGAC 
GAGGTCCGAG CGGCCGAGCC TGACCCCGAC TACGAGCAGA TGCAGCGTTC GGCGGGCCGG 
ACGGGCGAGG CTGAGGGGAA CAAGGAGGGG ACTGGGGGGC CCACCAAGGA CGGGGCGGCT 
TCTCAGGCAG GAGCCGAAAC AACAGGAGGG TCCCCGACCC GAGCTGAGAA TGAGAAGGAC 
GCTGCCACTG AGAAAAGCAA GAAGAGGGGG TTCTTGTTCA AAGCCAAGAA GGCAGCCATG 
ATGACGCAAC CACCCGCCAC CCCCGCTCTG CCCCGACTCC CTCACGACGT GGTGTCCGCG 
GACAACAGGG ATGACCCTGA GATTATCCTC AACACCACCA CGTATTACTA CTCTGTCCGG 
GTGTTTGCCG GCCAGGAGCC CAGCTCAGTG TGGGTGGGCT GGGTCACCCC TGACTACCAC 
CAGCACGACA TGAATTTTGA TCTGACCAAA GTCCGCGTTG TGACGGTGAC CATGGGGGAC 
GAGCGAGGCA ATGTACACAA CAGCTTGAAG CGCAGTAACT GCTACATGGT GTGGGGTGGG 
GATTTTGTGA GCCCGGGACA GCAAGGACGC ATCAGTCACA CAGACCTGGT CATCGGCTGC 
CTGGTGGACC TGGCCACCGG CCTCATGACC TTCACGGCCA ATGGAAAAGA GAGCAACACC 
TTTTTCCAGG TGGAGCCAAA CACGAAGCTG TTTCCAGCGG CCTTCGTCCT TCCCACCAAC 
CAAAATGTTA TGCAATTTGA ACTGGGAAAG CTGAAGAACA TCATGCCCCT GTCAGCAGCC 
ATGTTCCTGA GTGAACGGAA GAACCCAGCG CCGCAGTGTC CCCCCCGCTT GGAGGTACAA 
ATGCTCATGC CCGTGTCCTG GAGCAGGATG CCCAACCACT TCCTACAAGT GGAGACACAC 
CGGGCAGGTG ACCGCCTGGG CTGGGCTGTG CAGTGTGAGG AGCCTCTCAC CATGATGGCC 
TTGCATATCC CTGAGGAGAA CCGGGGAGGC CATTGGCCTA CGATGGTGGG AGCCTCGACT 
TCCACACCGA GGGACCTGGA ACTGGTCCTG CAGGTGATGG GCATCTTCGG GGACGAGGAC 
GTCAAGCAAA TCCTGAGGAT GATTGAGCCT GAAGTTTTCA AGGAAGAGGA AGAGGAGGAA 
GAGGAGGAGG AGGAGGAGGA GGATGAAGAA GAGAAGGAAG AGGATGAAGA AGAAGAGGAA 
GAAGAAAAAG AGGAGGAGGT GAAAGAGGAG GAGGAGGAAG AGGAAGAGAA GGGAGAGGAA 
GAAGAAGCTG TGGAGGAAGG ACTACTTCAG ATGAAGCTGC CTGAGTCTGT CAAGCTGCAG 
ATGTGCCATC TGCTGGAGTA CTTCTGTGAC CAGGATCTGC AGCACCGAGT GGAGGCGCTC 
ATTGCGTTCT CGGAGCGCTA CGTCAGTTGC CTGCAGAACA ACCAGCGCAG CCGCTACGGG 
CTGCTCATAA AGGCCTTCAC TATGTCCGCA GCGGAGACTG CCCGGCGCAC CCGGGAGTTT 
CGCTCTCCGC CCCAGGAGCA GATCAACATG CTTCTGCACT TCAAAGGGAG TGAAGACGAG 
GAGGACTGTC CGGTCCCAGA CGAAATTCGG CAGGAGCTGC TGGATTTTCA CCAGGACCTG 
CTGCTGCACT GCGGCATCCA GCTGGAAACG GAGGAGGAGG AGGTGGAAGA GGAGACCACC 
CTGGGAGGCC GGCTCATGAG CTTTTTGGAG AAGGTGAAGC TGGTGAAGAA GAAGGAGAAG 
GAGCCAGATG AGACCCCTCC CCCAGAAGAG AAAAAGCCTG AGTCCTTGCA GGAGCTGGTC 
TCTCACACAA TGGTGCGCTG GGCCCAAGAG GACTTTATCC AGAACCCAGA GCTGGTCCGG 
GCCATGTTCA GCCTCCTCCA CCGGCAGTAC GATGGCCTCG GGGAGCTGCT CCGTGCCCTG 
CCCAGGGCTT ACACCATCTC CCCGTCCTCC GTGGAGGACA CCATGAGCCT CCTGGAAAGC 
CTGTGTCAGA TCCGCTCCTT GCTCATTGTG CAGATGGGGC CGCAAGAGGA GAATCTCATG 
ATCCAGAGCA TTGGGAATAT CATGAACAAC AAGGTGTTTT ACCAGCACCC AAATCTGATG 
AGGGCTTTGG GGATGCACGA AACAGTGATG GAAGTTATGG TCAATGTGTT AGGAGGGGGA 
GAGTCTAAGG AAATCCGCTT CCCCAAAATG GTCACCAGCT GCTGCCGGTT CCTCTGCTAT 
TTCTGTCGCA TTAGTCGCCA GAACCAAAGG TCCATGTTTG ATCACCTCAG CTACCTGCTG 
GAGAACAGCG GGATCGGCCT GGGTATGCAG GGTTCCACTC CGCTAGATGT GGCGGCTGCC 
TCCGTCATAG ACAACAACGA GCTGGCCTTG GCCCTTCAGG AACAAGACCT GGAAAAGGTC 
GTCTCCTACT TGGCCGGCTG CGGCCTTCAG AGCTGCCCCA TGCTGCTGGC CAAGGGCTAC 
CCCGACATTG GCTGGAACCC CTGCGGGGGG GAGCGCTATC TGGATTTCCT GAGATTTGCC 
GTCTTTGTTA ATGGAGAGAG CGTGGAAGAG AATGCGAATG TTGTGGTTCG TCTGCTCATC 
CGGCGTCCCG AATGCTTCGG CCCGGCCCTG CGAGGAGAGG GCGGCTCTGG GCTGCTGGCT 
GCTATGGAAG AGGCAATCCG GATCTCAGAG GATCCTGCTC GAGATGGCCC GGGCGTCAGG 
AAGGACCGGA GGCGGGAGCA CCACGGGGGT GAAGAGGCCC CTGAAGAAAA CCGTGTGCAC 
TTGGGAAATG CCATCATGTC ATTCTATTCC GCCCTGATCG ACCTGCTGGG ACGCTGTGCT 
CCAGAGACTC ATCTGATCCA GGCCGGGAAG GGAGAAGCGC TGAGGATCCG AGCCATTCTG 
CGCTCCCTGG TGCCCCTGGA CGACCTCGTG GGCATTATCA GCCTGCCCCT GCAGATCCCC 
ACCCTGGGCA AAGATGGGGC CCTGGTGCAG CCTCGGATGT CTGCCTCCTT CGTGCCTGAC 
CACAAAGCCC CCATGGTCCT CTTCCTGGAC CGCGTCTACG GCATCGACAG CCAGGACTTC 
CTGCTCCACG TGCTTGATGT CGGCTTCCTG CCTGACATGC GGGCGGCTGC CTCCCTGGAC 
ACGGCAACCT TCAGCACCAC GGAGATGGCC CTGGCCTTGA ACCGCTACCT GTGCACAGCC 
GTGCTTCCCC TGATCACCAA GTGCGCCCCA CTGTTTGCGG GGACGGAGCA CCGCGCCATC 
ATGGTGGACT CCATGCTCCA CACGGTGTAC CGCCTGTCCC GGGGACGCTC TCTCACCAAG 
GCCCAGCGAG ATGTCATTGA GGACTGCCTC ATGGCGCTCT GCAGGTACAT CCGTCCATCT 
ATGCTCCAGC ACCTTCTGCG TCGTCTGGTA TTCGACGTTC CCATCCTCAA CGAGTTTGCC 
AAGATGCCCC TCAAGCTCCT GACCAATCAC TATGAGCGCT GCTGGAAATA TTACTGCCTC 
CCTACGGGCT GGGCCAACTA TGGAGTTACC TCCGAGGAAG AGCTGCATCT GACTCGCAAG 
CTTTTCTGGG GCATCTTTGA GTCACTGGCC CACAAGAAAT ACGACCAGGA GCTCTACCGC 
ATGGCCATGC CGTGTCTGTG CGCCATCGCC GGGGCCCTGC CCCCCGACTA CGTGGATGCC 
TCCTACTCCT CCAAAGCTGA GAAGAAGGCC ACTGTGGATG CTGAGGGCAA CTTTGACCCC 
CGGCCCGTGG AGACACTCAA TGTGATCATA CCCGAGAAAC TCGACTCCTT CATCAACAAA 
TTTGCCGAGT ACACACACGA GAAGTGGGCA TTTGACAAGA TTCAGAACAA TTGGTCATTT 
GGGGAGAACA TTGACGAGGA GCTCAAGACC CACCACATGC TTCGGCCCTA CAAGACCTTC 
TCAGAAAAGG ACAAGGAGAT TTACCGATGG CCCATCAAGG AGTCCCTGAA GGCCATGATC 
GCCTGGGAGT GGACTGTGGA AAAGGCCCGT GAGGGTGAGG AGGAGAAGGG AGAGAAGAAG 
ACCAAGACTC GCAAGATCTC CCAGAATACC CCGGCCACCT ATGACCCAAG AGAGGGCTAT 
AATCCCCAGC CTCCAGACCT TACCTGTGTC ACCCTGTCCC GGGAACTGCA GGCCATGGCT 
GAACAACTGG CTGAGAATTA CCATAACACA TGGGGGAGGA AGAAAAAGCA GGAATTAGAA 
GCCAAGGGTG GAGGCACCCA CCCCCTCCTG GTCCCCTATG ACACACTGAC GGCCAAGGAG 
AAGGCTCGCG ATAGAGAGAA AGCCCAGGAA CTGCTCAAAT TCCTGCAGAT GAACGGCTAC 
GCGGTGACGC GGTGCCCCCA GGCCTTCCTC CATCTCCCCC GAGCCCTGTC CTGTTCCCCC 
AGGACCGTGA TGAAGTCTGG TCCCGAGATC GTCAAGGCTG GTCTCCGATC GTTCTTCGAG 
AGCGCTTCGG AGGACATCGA GAAGATGGTG GAGAACCTGC GCCTGGGCAA GGTGTCCCAG 
GCGCGGACGC AGGTCAAGGG CGTCGGCCAG AACCTCACCT ACACCACCGT GGCTCTGCTT 
CCCGTTCTCA CCACGCTTTT CCAGCACATC GCCCAGCACC AGTTTGGGGA TGATGTCATC 
TTGGACGACG TCCAGGTCTC CTGCTACCGA ACACTTTGCA GCATCTACTC TCTGGGGACC 
ACCAAGAATT CCTATGTGGA GAAGCTCCGT CCAGCCCTTG GAGAATGCCT TGCCCGTCTG 
GCAGCTGCTA TGCCAGTTGC CTTCCTTGAA CCCCAACTGA ATGAGTTCAA CGCCTGCTCT 
GTCTACACCA CCAAATCTCC CCGAGAACGT GCCATCCTGG GCCTTCCGAG CTCCGTGGAG 
GAGATGTGCC CAGATATCCC AGACCTGAAG CACCTGATTG CCGAGATCGG GGGGCGGGCC 
GAGTCGGGCG CCCGTTACAC CGAGATGCCC CATGTCATCG AGATCACGCT GCCGATGCTC 
TGTAACTACC TGCCCCGCTG GTGGGAGCGG GGGCCCGAGG TCCCCTCCTC ACAGCCCCCA 
GGGGGACCCC CACCCTGCAC AGATGTCACC TCGGACCACC TCAACTCCCT CCTGGGAAAC 
ATCCTCCGGA TCATTGTCAA CAACCTGGGC ATTGACGAGG CTTCGTGGAT GAAGCGCTTG 
GCAGTGTTTG CGCAGCCGAT CGTGAGCCGG GCCCGGCCCG AGCTGCTCCG CTCACATTTC 
ATCCCCACGG TGGGCCGGCT CCGGAAGCGC GCAGGGAAGG TGGTGGCCGA GGAAGAGCAG 
CTGAGGCTGG AAGCCAAGGC GGAGGCTGAG GAGGGAGAGC TCCTGGTGCG GGACGAGTTC 
TCTGTGCTCT GCCGGGATCT CTACGCACTC TACCCCCTGC TCATCCGCTA CGTAGACAAC 
AACCGAGCCC ACTGGCTAAC AGAACCCAAC CCTGATGCAG AAGAACTCTT CAGGATGATA 
GCAGAAATCT TCATCTATTG GTCCAAGTCT CATAACTTCA AGAGAGAAGA ACAGAATTTC 
GTGGTACAGA ACGAAATCAA TAACATGTCC TTCCTGACGG CAGATAGCAA GAGCAAGATG 
GCCAAGTCAG GAGGCTCCGA TCAAGAGAGA ACAAAGAAGA AACGGCGAGG GGACCGATAT 
TCCGTCCAGA CCTCTCTGAT CGTGGCTACC CTCAAGAAGA TGCTGCCTAT TGGGTTGAAC 
ATGTGTGCAC CCACTGACCA GGACCTCATC GCGCTGGCCA AGACTCGTTA TGCCCTGAAA 
GACACAGATG AAGAAGTTCG GGAATTTCTG CAGAATAACC TTCATCTTCA GGGAAAGAGT 
GAGGGCTCCC CATCACTGCG CTGGCAGATG GCCCTGTACC GGGGGCTGCC TGGCCGGGCT 
GAGGATGCTG ATGCCCCTGA GAACATTGTT CGCCGAGTAC AGGAAGTCTC AGCGGTGCTT 
TACCACCTGG AACAGACAGA ACATCCCTAC AAGTCCAAGA AAGCTGTGTG GCACAAGCTG 
CTTTCAAAAC AGCGTCGTCG GGCGGTTGTG GCTTGTTTTC GCATGACACC CTTGTACAAC 
TTGCCTCGGC ATCGGGCAAG CAATATGTTC TTGGAGTCCT ACAAAGCATC ATGGATCCTA 
ACTGAGGAGC ACAGTTTTGA GGACCGCATG ATTGATGACC TTTCTAAAGC TGGGGAGGAA 
GAAGAAGAGG AAGAAGAACA GGAAGAGAAG AAGCCAGACC CTCTTCACCA GCTGGTATTA 
CACTTTAGCC GGACAGCCCT GACAGAGAAG AGCAAACTAG ATGAGGACTA TTTGTACATG 
GCATATGCTG ACATCATGGC CAAGAGCTGC CACTTGGAGG AGGAAGAGGA GCCTTCAGAA 
CCTGCTGAGG AGGAGGCTGA AGTTTCATTT GAGGAGAAGG AGATGGAAAA GCAGAAACTG 
CTCTACCAGC AGTCAAGGCT ACACAACCGA GGGGCTGCTG AGATGGTGCT ACAAATGATT 
AGTGCCTGCA AAGGGGAGCC GGGAGTCATG GTTTCATCCA CCCTGAAGTT GGGCATCTCC 
ATCCTCAACG GTGGCAACAT GGAGGTGCAG CAGAAGATGC TGGACTATCT GAAGGACAAA 
AAGGATGTTG GCTTCTTCCA GAGCATCCAG GCCCTGATGC AGACATGCAG TGTTCTTGAT 
CTGAATGCCT TTGAGAGGCA GAACAAGGCA GAGGGGCTTG GAATGGTGAC CGAGGAAGGG 
ACTGGGGAAA AAGTAATGGC CGACGATGAG TTCACCCAGG ATCTCTTCCG CTTTCTGCAG 
CTGCTGTGTG AAGGCCATAA CAATGACTTT CAGAACTACC TCCGCACGCA GACGGGGAAC 
ACGACCACCA TCAACATCAT CATCTGCACA GTGGATTATC TGCTGAGGCT GCAGGAGTCC 
ATCAGTGACT TCTACTGGTA CTACTCGGGG AAGGATGTGA TCGAAGAGCA AGGGAAGAGG 
AACTTCTCCA AAGCCATGGC CGTGGCCAAG CAGGTCTTCA ACAGCCTCAC CGAGTACATC 
CAGGGCCCGT GCACAGGAAA CCAGCAGAGC CTGGCCCACA GCCGCCTGTG GGATGCCGTG 
GTCGGCTTCC TCCACGTTTT TGCCCACATG ATGATGAAGC TGGCTCAGGA CTCAAGCCAG 
ATTGAGCTGC TGAAGGAGCT CCTGGATCTG CAGAAAGACA TGGTGGTCAT GTTGCTTTCG 
CTGCTGGAAG GTAACGTGGT CAACGGCACA ATTGCCCGGC AGATGGTGGA CATGCTGGTG 
GAGTCCTCCT CCAACGTGGA GATGATCCTC AAGTTCTTCG ACATGTTCCT AAAGCTGAAG 
GACATCGTGG GCTCCGAGGC ATTCCAGGAC TACGTCACCG ACCCCCGTGG ACTCATCTCC 
AAGAAGGATT TCCAGAAGGC CATGGACAGC CAGAAGCAGT TCACTGGCCC AGAGATCCAG 
TTCCTCCTGT CCTGCTCAGA GGCGGATGAG AACGAGATGA TCAATGTGGA GGAATTTGCC 
AGCCGCTTCC AGGAGCCGGC CCGAGACATC GGCTTCAACG TGGCCGTGCT GCTGACCAAC 
CTGTCGGAGC ACGTGCCCCA CGACCCGCGC CTCAAGAACT TCCTGGAGCT GGCTGAGAGC 
ATCCTGGAGT ACTTCCGGCC CTACCTGGGC CGGATCGAGA TCATGGGGGC CTCCCGGCGC 
ATCGAGCGCA TCTACTTTGA AATCTCTGAG ACCAACAAAG CACAGTGGGA GATGCCCCAG 
GTGAAGGAGT CCAAACGCCA GTTCATCTTT GACGTGGTGA ATGAAGGGGG GGAGTCGGAG 
AAGATGGAAA TGTTTGTGAG CTTCTGTGAA GACACCATCT TTGAGATGCA GATCGCCGCC 
CAGATCTCAG AGCCCGAGGG AGAGCCTGAG GAAGATGAGG AGGAGTCCAT GGCAGAGTCG 
GGGCCAGACT CCGGGGAGGC AGGGCCCGAC GGAGAAGGGG CCGGCGCAGG GGGCCCGACG 
GGCATCTCCG GGCACGTGGC CGGTGCCGTC TCCTCGGTCC TGCGGAAGCT GACCTACCGG 
AGCCTGCGCA GGAGGGTGCG GAGGCTCCGC AAGCTGACCC TGCGGGAGGC TGCAGGAACC 
GGGGCCGGGC TCATCTGGGC CGGGGCCACG TGGACGGGCT CCGGAGTGAT CGGCGCCACC 
CGGGGGGCCC TGGGCCTACT CTGGGGCTCT CTGTTTGGGG GAGGGCTGGT GGAGGGAGCC 
AAGAAAGTGA CCGTGACGGG ACTGTTGGCC GGGATGCCAG ACCCCACAGG CGATGAGGTC 
CATGGGGAGC AGGCGGCTGG CGCCGGGGCT GCCGGGACTG GGGACGGAGC CGTGGAGGGT 
GATGAGGAGG TGGGGGCGGA GTTGGCCGAG AGGGAGGCTG AGGCTGAGGC CGGAGCTGGG 
GAGGCCGGGC CAGCCGGAGG AGAGGGAGCC GGGGCCAATG GAGGCCACTT CCGGATGGAG 
GTCCCCGGAG GCCTGGGTGA CATGGGAGAC ACAACACCCG TTGAGCCGCC CACTCCTGAG 
GGTTCCCCCA TCTTCCGGAG GAAACTAGGG CTGGAGGGAG AAGAAGAACA AGCCTTAGAA 
CCAGAACCAG AGCCAGAACC AGAACCAGAG CCAGAGCCAG AACCCGAGCC TGAGAAAGCT 
GATGCTGAGA ACGGAGAAAA GGAAGGGTCC CAAGAGCCAG AACCTGAGGC TCCCAAGAAG 
GCAGCTGCCC CACCTCCGCC CCGGAAGGAG GACGCCAAAT CTGAGGGCCC TGACTTCTGG 
GAGGAAATGG AGGTGCAGAG GGTCAAGTTC CTGAACTACC TCTCCAGAAA CTTCTATACC 
CTGAGATTCC TCGCCCTCTT CCTGGCCTTT GCCATCAACT TCATCCTGCT CTTCTACAAG 
GTGTCTGACT CTCCGCCAGG AGAAGAGGAC CTGGAAGGCT CCGGTCTCGA GGGTCTGGCA 
GGCTCAGGGT CCGGGGACTT CCTGGGATCC GGCATGGGCT CCGGGGCTGG GGAGGATCTG 
GAGAACGAGG AGGAAGAGAA CATGGTCTAC TACTTCCTGG AAGAGAGTAC GGGCTACATG 
GAGCCAGCCC TGCGAGGTCT CAGCATCCTG CACACCCTCA TTGCCTTCCT CTGCATCATA 
GGCTACAACT GTCTCAAGGT ACCGCTGGTT ATATTTAAAC GGGAGAAGGA GCTAGCCCGG 
AAACTAGAGT TCGATGGCCT TTACATCACT GAGCAGCCCG AGGATGACGA CGTGAAGGGC 
CAGTGGGATC GACTGGTGCT TAACACCCCG TCCTTCCCCA GCAACTACTG GGACAAGTTC 
GTGAAAAGGA AGGTGCTGGA GAAACATGGC GACATCTTTG GCCGGGAGCG GATTGCAGAG 
CTGTTGGGCA TGGATCTGGC CAGCCTGGAG ATCACGGCGC AGAACGAGAA GCGACCGGAA 
CCCCCCTCGG GACTGCTCAC CTGGATCATG TCCATAGATG TCAAATACCA AATCTGGAAA 
TTTGGTGTCA TCTTTACAGA TAATTCATTC TTGTATTTGA CGTGGTACAT GGTCATGTCC 
CTCCTCGGCC ACTATAACAA CTTCTTCTTT GCCGCTCACC TGCTGGACAT CGCCATGGGA 
GTCAAGACGC TTCGGACCAT CCTGTCTTCT GTCACCCACA ATGGGAAGCA GCTGGTGATG 
ACGGTGGGCC TGCTGGCCGT CGTGGTTTAC CTCTACACGG TTGTGGCCTT CAATTTCTTC 
CGCAAATTTT ACAATAAAAG CGAGGATGAG GATGAGCCGG ACATGAAATG TGATGACATG 
ATGACGTGTT ACCTGTTCCA CATGTACGTG GGGGTCCGAG CTGGTGGGGG CATCGGGGAT 
GAGATTGAGG ATCCAGCAGG GGATGAATAC GAGCTCTACC GTGTGGTCTT TGACATCACC 
TTTTTCTTCT TCGTCATCGT CATCCTGCTG GCCATTATCC AAGGTCTGAT CATTGATGCT 
TTTGGGGAGC TCCGAGACCA GCAAGAGCAA GTGAAGGAAG ATATGGAGAC CAAATGTTTC 
ATCTGTGGCA TTGGAAGTGA CTACTTTGAT ACAACACCCC ACGGTTTTGA GACCCACACG 
CTGGAGGAGC ACAACCTGGC TAACTACATG TTTTTCCTGA TGTATCTGAT CAATAAGGAT 
GAGACCGAGC ACACGGGACA GGAGTCGTAT GTCTGGAAGA TGTACCAGGA GCGCTGCTGG 
GACTTCTTCC CAGCTGGTGA CTGTTTCCGA AAACAGTATG AAGACCAGCT GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023355732.1
CDS	106..14322
Translation	MGDGGEGEDDVQFLRTEDEVVLQCSATTLKEQIKLCLAAEGFGNRLCFLEPTSNAQNVPPDLAVCCFVLEQSLSVRALQEMLANTVEAGTESSQGGGHRTLLYGHAILLRHAHSQMYLSCLTTSRSMTDKLAFDVGLQEDATGEACWWTMHPASKQRSEGEKVRVGDDLILVSVSSERYLHLSTASGELQADASFMQTLWNMNPICSGCEEGYVTGGHVLRLFHGHMDECLTISSADSDDQRRLVHYEGGAVCTHARSLWRLEPLRISWSGSHLRWGQPLRVRHVTTGRYLALTEDQGLIVVDASKAATKATAFCFRISKEKLDTAPKRDVEGMGPPEIKYGESLCFVQHVASGLWLTYAAPDPKALRLGLMKRKAMLHQEGHMDDALSLTRCQLEESQAARMIYSTAGLYNQFIKGLDSLSGKPKGAPAAGVTLPIEGVILSLQDLIGYFEPPSEELQHEEKQSKLRSLRNRQSLFQEEGMLTLVLNCIDRLNFYSTAAHFAEFAGEEAAESWKEIVNLLYELLASLIRGNRTNCALFSHNLDWLVSKLDRLEASSGILEVLYCVLIESPEVLNIIQENHIKSIISLLDKHGRNHKVLDVLCSLCVCNGVAVRSNQVLITENLLPGRELLLQTNLINYVTSIRPNIFVGRAEGTTQYGKWYFEVMVDSVEPFLTAQATHLRVGWALTEGYSPYPGGGEGWGGNGVGDDLYSYGFDGLHLWSGRIARPVASPGQHLLAAEDVVSCCLDLSVPSISFRINGCPVQGVFEAFNLDGLFFPVVSFSAGVRVRFLLGGRHGEFKFLPPPGYAPCHEALLPRERLRLEPIKEYRREGPHGPHLLGPTCCLSHTDFVPYPVDTVQIVLPPHLERIREKLAENIHELWALTRIEQGWTYGPVRDDNKRLHPCLVDFHSLPEPERNYNLQMSGETLKTLLALGCHVGMADEKAEENLKKTKLPKTYMMnullnullnullnullnullnullnullnullnullDLSHVRLTPAQTTLVDRLAENGHNVWARDRVGQGWS*SAVQDIPARRNPRLVPYRLLDEATKRSNRDSLCQAVRTLLGYGYNIEPPDQESSQVESQTSGDRVRIFRAERSYAVQSGRWYFEFEAVTTGEMRVGWARPELRPDVELGADDLAYVFNGHRGQRWHMGSEPFGRPWQPGDVVGCMIDLSENNILFTLNGEVLMSDSGSETAFKDIEIGDGFLPVCSLGLGQVGRLNLGQDVGSLRYFTICGLQEGFEPFAINMQRPVTTWFSKSLPQFEPMPPDHPHYEVTRVDGTVDTPPCLRLTHRTWGSQNSLVEMMFLRLSLPVQFYQHFRCTPGATPLAPPGPQPPGDDEVRAAEPDPDYEQMQRSAGRTGEAEGNKEGTGGPTKDGAASQAGAETTGGSPTRAENEKDAATEKSKKRGFLFKAKKAAMMTQPPATPALPRLPHDVVSADNRDDPEIILNTTTYYYSVRVFAGQEPSSVWVGWVTPDYHQHDMNFDLTKVRVVTVTMGDERGNVHNSLKRSNCYMVWGGDFVSPGQQGRISHTDLVIGCLVDLATGLMTFTANGKESNTFFQVEPNTKLFPAAFVLPTNQNVMQFELGKLKNIMPLSAAMFLSERKNPAPQCPPRLEVQMLMPVSWSRMPNHFLQVETHRAGDRLGWAVQCEEPLTMMALHIPEENRGGHWPTMVGASTSTPRDLELVLQVMGIFGDEDVKQILRMIEPEVFKEEEEEEEEEEEEEDEEEKEEDEEEEEEEKEEEVKEEEEEEEEKGEEEEAVEEGLLQMKLPESVKLQMCHLLEYFCDQDLQHRVEALIAFSERYVSCLQNNQRSRYGLLIKAFTMSAAETARRTREFRSPPQEQINMLLHFKGSEDEEDCPVPDEIRQELLDFHQDLLLHCGIQLETEEEEVEEETTLGGRLMSFLEKVKLVKKKEKEPDETPPPEEKKPESLQELVSHTMVRWAQEDFIQNPELVRAMFSLLHRQYDGLGELLRALPRAYTISPSSVEDTMSLLESLCQIRSLLIVQMGPQEENLMIQSIGNIMNNKVFYQHPNLMRALGMHETVMEVMVNVLGGGESKEIRFPKMVTSCCRFLCYFCRISRQNQRSMFDHLSYLLENSGIGLGMQGSTPLDVAAASVIDNNELALALQEQDLEKVVSYLAGCGLQSCPMLLAKGYPDIGWNPCGGERYLDFLRFAVFVNGESVEENANVVVRLLIRRPECFGPALRGEGGSGLLAAMEEAIRISEDPARDGPGVRKDRRREHHGGEEAPEENRVHLGNAIMSFYSALIDLLGRCAPETHLIQAGKGEALRIRAILRSLVPLDDLVGIISLPLQIPTLGKDGALVQPRMSASFVPDHKAPMVLFLDRVYGIDSQDFLLHVLDVGFLPDMRAAASLDTATFSTTEMALALNRYLCTAVLPLITKCAPLFAGTEHRAIMVDSMLHTVYRLSRGRSLTKAQRDVIEDCLMALCRYIRPSMLQHLLRRLVFDVPILNEFAKMPLKLLTNHYERCWKYYCLPTGWANYGVTSEEELHLTRKLFWGIFESLAHKKYDQELYRMAMPCLCAIAGALPPDYVDASYSSKAEKKATVDAEGNFDPRPVETLNVIIPEKLDSFINKFAEYTHEKWAFDKIQNNWSFGENIDEELKTHHMLRPYKTFSEKDKEIYRWPIKESLKAMIAWEWTVEKAREGEEEKGEKKTKTRKISQNTPATYDPREGYNPQPPDLTCVTLSRELQAMAEQLAENYHNTWGRKKKQELEAKGGGTHPLLVPYDTLTAKEKARDREKAQELLKFLQMNGYAVTRCPQAFLHLPRALSCSPRTVMKSGPEIVKAGLRSFFESASEDIEKMVENLRLGKVSQARTQVKGVGQNLTYTTVALLPVLTTLFQHIAQHQFGDDVILDDVQVSCYRTLCSIYSLGTTKNSYVEKLRPALGECLARLAAAMPVAFLEPQLNEFNACSVYTTKSPRERAILGLPSSVEEMCPDIPDLKHLIAEIGGRAESGARYTEMPHVIEITLPMLCNYLPRWWERGPEVPSSQPPGGPPPCTDVTSDHLNSLLGNILRIIVNNLGIDEASWMKRLAVFAQPIVSRARPELLRSHFIPTVGRLRKRAGKVVAEEEQLRLEAKAEAEEGELLVRDEFSVLCRDLYALYPLLIRYVDNNRAHWLTEPNPDAEELFRMIAEIFIYWSKSHNFKREEQNFVVQNEINNMSFLTADSKSKMAKSGGSDQERTKKKRRGDRYSVQTSLIVATLKKMLPIGLNMCAPTDQDLIALAKTRYALKDTDEEVREFLQNNLHLQGKSEGSPSLRWQMALYRGLPGRAEDADAPENIVRRVQEVSAVLYHLEQTEHPYKSKKAVWHKLLSKQRRRAVVACFRMTPLYNLPRHRASNMFLESYKASWILTEEHSFEDRMIDDLSKAGEEEEEEEEQEEKKPDPLHQLVLHFSRTALTEKSKLDEDYLYMAYADIMAKSCHLEEEEEPSEPAEEEAEVSFEEKEMEKQKLLYQQSRLHNRGAAEMVLQMISACKGEPGVMVSSTLKLGISILNGGNMEVQQKMLDYLKDKKDVGFFQSIQALMQTCSVLDLNAFERQNKAEGLGMVTEEGTGEKVMADDEFTQDLFRFLQLLCEGHNNDFQNYLRTQTGNTTTINIIICTVDYLLRLQESISDFYWYYSGKDVIEEQGKRNFSKAMAVAKQVFNSLTEYIQGPCTGNQQSLAHSRLWDAVVGFLHVFAHMMMKLAQDSSQIELLKELLDLQKDMVVMLLSLLEGNVVNGTIARQMVDMLVESSSNVEMILKFFDMFLKLKDIVGSEAFQDYVTDPRGLISKKDFQKAMDSQKQFTGPEIQFLLSCSEADENEMINVEEFASRFQEPARDIGFNVAVLLTNLSEHVPHDPRLKNFLELAESILEYFRPYLGRIEIMGASRRIERIYFEISETNKAQWEMPQVKESKRQFIFDVVNEGGESEKMEMFVSFCEDTIFEMQIAAQISEPEGEPEEDEEESMAESGPDSGEAGPDGEGAGAGGPTGISGHVAGAVSSVLRKLTYRSLRRRVRRLRKLTLREAAGTGAGLIWAGATWTGSGVIGATRGALGLLWGSLFGGGLVEGAKKVTVTGLLAGMPDPTGDEVHGEQAAGAGAAGTGDGAVEGDEEVGAELAEREAEAEAGAGEAGPAGGEGAGANGGHFRMEVPGGLGDMGDTTPVEPPTPEGSPIFRRKLGLEGEEEQALEPEPEPEPEPEPEPEPEPEKADAENGEKEGSQEPEPEAPKKAAAPPPPRKEDAKSEGPDFWEEMEVQRVKFLNYLSRNFYTLRFLALFLAFAINFILLFYKVSDSPPGEEDLEGSGLEGLAGSGSGDFLGSGMGSGAGEDLENEEEENMVYYFLEESTGYMEPALRGLSILHTLIAFLCIIGYNCLKVPLVIFKREKELARKLEFDGLYITEQPEDDDVKGQWDRLVLNTPSFPSNYWDKFVKRKVLEKHGDIFGRERIAELLGMDLASLEITAQNEKRPEPPSGLLTWIMSIDVKYQIWKFGVIFTDNSFLYLTWYMVMSLLGHYNNFFFAAHLLDIAMGVKTLRTILSSVTHNGKQLVMTVGLLAVVVYLYTVVAFNFFRKFYNKSEDEDEPDMKCDDMMTCYLFHMYVGVRAGGGIGDEIEDPAGDEYELYRVVFDITFFFFVIVILLAIIQGLIIDAFGELRDQQEQVKEDMETKCFICGIGSDYFDTTPHGFETHTLEEHNLANYMFFLMYLINKDETEHTGQESYVWKMYQERCWDFFPAGDCFRKQYEDQLA

Target ORF information:

RefSeq Version	XM_023499964.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ryanodine receptor 1 (RYR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023499964.1

ORF Insert Sequence:

ATGGGGGATG GAGGGGAGGG CGAGGATGAC GTCCAGTTTC TCCGAACTGA AGATGAGGTT 
GTCCTGCAGT GCAGCGCTAC CACCCTCAAG GAGCAAATCA AGCTCTGCCT AGCCGCCGAA 
GGCTTTGGAA ACCGACTCTG TTTTTTGGAA CCTACCAGCA ATGCCCAGAA TGTGCCCCCT 
GACTTGGCTG TGTGCTGCTT TGTGCTGGAA CAATCTCTCT CGGTGCGAGC CCTGCAGGAG 
ATGCTGGCCA ACACAGTGGA GGCTGGCACT GAGTCATCTC AGGGCGGGGG ACACCGGACC 
CTTCTCTATG GCCACGCCAT CTTACTCCGG CATGCCCACA GCCAAATGTA CCTAAGCTGC 
CTCACAACAT CCCGCTCTAT GACTGACAAA CTCGCCTTCG ATGTGGGCCT GCAAGAAGAT 
GCCACAGGGG AGGCCTGCTG GTGGACCATG CATCCGGCCT CAAAGCAGCG ATCCGAAGGA 
GAGAAGGTCC GGGTGGGGGA TGACCTCATC CTTGTCAGTG TCTCTTCTGA AAGATACTTG 
CACCTGTCCA CAGCCAGTGG GGAGCTCCAG GCTGATGCTT CTTTCATGCA AACCCTTTGG 
AACATGAACC CCATCTGTTC TGGGTGTGAG GAAGGGTACG TGACCGGTGG CCATGTTCTC 
CGGCTGTTCC ACGGCCACAT GGACGAGTGT CTAACCATCT CTTCAGCTGA CAGTGATGAC 
CAGCGCAGGC TGGTTCATTA TGAAGGGGGT GCTGTGTGCA CCCACGCCCG TTCCCTGTGG 
AGACTGGAAC CCCTGAGGAT AAGCTGGAGC GGGAGCCACC TGCGCTGGGG CCAGCCCCTG 
CGTGTGCGAC ACGTGACTAC AGGGAGATAC TTGGCACTGA CAGAGGACCA AGGCCTGATA 
GTGGTAGATG CCTCGAAGGC TGCCACCAAG GCAACTGCTT TCTGTTTCCG AATTTCTAAG 
GAGAAACTGG ACACAGCCCC AAAGCGAGAT GTGGAAGGCA TGGGCCCTCC CGAGATCAAG 
TATGGCGAGT CTCTGTGCTT TGTCCAACAC GTGGCCTCTG GCCTATGGCT CACCTACGCG 
GCCCCTGACC CCAAAGCCTT GCGCCTGGGC CTGATGAAGA GGAAGGCCAT GCTGCATCAG 
GAAGGCCACA TGGATGACGC TCTGTCCCTC ACTCGGTGCC AGCTGGAGGA GTCCCAGGCG 
GCCCGGATGA TCTACAGCAC AGCCGGGCTG TACAACCAGT TCATCAAGGG TCTGGACAGT 
CTGAGTGGGA AGCCCAAGGG GGCTCCGGCA GCAGGGGTGA CCCTGCCCAT AGAGGGGGTC 
ATCCTGAGCC TGCAGGACCT TATTGGCTAC TTCGAGCCCC CATCGGAAGA GCTCCAGCAT 
GAGGAGAAAC AGAGCAAACT GCGGAGCTTG CGGAATCGCC AGAGCCTCTT CCAGGAAGAG 
GGAATGCTGA CTTTGGTCCT GAATTGTATC GATCGGCTCA ACTTCTACTC CACGGCTGCC 
CACTTTGCTG AGTTTGCAGG AGAGGAGGCA GCTGAGTCTT GGAAAGAGAT TGTGAACTTA 
CTCTATGAGC TTTTGGCTTC TTTGATTCGG GGAAACCGTA CCAATTGTGC TCTCTTCTCC 
CACAACCTCG ACTGGCTTGT CAGCAAGTTG GATCGGCTCG AGGCCTCTTC TGGAATCCTG 
GAGGTCCTTT ATTGTGTACT GATTGAGAGT CCAGAAGTAC TGAACATTAT TCAGGAGAAT 
CACATCAAGT CCATCATTTC CCTGTTGGAC AAGCATGGCC GGAACCACAA GGTTCTGGAC 
GTGTTGTGCT CTCTGTGCGT GTGTAATGGC GTTGCTGTCC GCTCCAACCA GGTCCTGATC 
ACCGAGAACC TCCTTCCCGG GCGTGAGCTC CTACTACAGA CCAACCTCAT TAACTATGTC 
ACCAGCATAC GCCCTAATAT CTTCGTGGGC CGGGCTGAGG GGACCACACA GTATGGGAAG 
TGGTACTTTG AAGTCATGGT AGACTCTGTG GAACCTTTCC TGACTGCTCA AGCCACACAC 
CTGAGAGTGG GCTGGGCCCT CACTGAGGGA TACAGCCCCT ACCCTGGTGG TGGGGAAGGC 
TGGGGTGGCA ATGGCGTTGG AGATGACCTC TACTCCTATG GCTTTGATGG ACTGCACCTC 
TGGTCTGGGA GGATTGCCCG GCCAGTGGCC TCTCCAGGCC AGCACCTCCT GGCTGCCGAG 
GATGTAGTTA GCTGCTGTCT GGATCTGAGT GTCCCATCCA TCTCATTCCG AATCAATGGC 
TGCCCCGTGC AAGGCGTCTT TGAGGCTTTC AACCTGGATG GGCTCTTCTT CCCAGTGGTC 
AGCTTCTCTG CTGGTGTTCG GGTACGGTTT CTCCTGGGTG GTCGCCATGG AGAGTTCAAA 
TTCCTCCCCC CACCGGGCTA TGCCCCATGC CATGAAGCTC TACTACCCCG TGAGCGCCTC 
CGGCTGGAGC CCATTAAGGA GTACCGTCGA GAAGGACCCC ATGGGCCTCA CCTCTTGGGA 
CCTACTTGCT GCTTGTCCCA CACCGACTTT GTGCCCTACC CTGTGGACAC TGTCCAGATT 
GTTTTGCCAC CTCATCTGGA ACGGATCCGA GAGAAACTGG CAGAGAATAT CCATGAGCTC 
TGGGCCCTGA CTCGCATCGA GCAAGGATGG ACCTATGGCC CGGTACGGGA TGATAACAAG 
CGGCTTCACC CATGCCTGGT GGACTTTCAC AGCCTCCCAG AGCCTGAGAG AAACTACAAT 
CTGCAGATGT CAGGAGAGAC ACTCAAGACC CTGCTGGCTT TGGGATGTCA TGTAGGCATG 
GCTGATGAGA AGGCTGAGGA AAACCTCAAG AAGACAAAAC TCCCCAAGAC GTACATGATG 
AGNNNNNNNN NNNNNNNNNN NNNNNTGGAC CTGAGCCACG TGCGGTTAAC CCCAGCACAG 
ACGACGCTCG TTGACCGGCT GGCTGAGAAT GGGCACAATG TGTGGGCGAG AGACCGTGTG 
GGGCAAGGCT GGAGTTAAAG TGCTGTCCAA GACATCCCTG CCCGGAGGAA CCCCCGGCTT 
GTCCCCTACC GCCTCCTGGA TGAGGCTACC AAGCGCAGCA ATCGAGACAG CCTCTGCCAA 
GCAGTGCGCA CCCTCTTGGG TTATGGCTAT AACATTGAGC CACCTGACCA GGAATCCAGC 
CAAGTGGAGA GCCAGACCAG TGGGGACCGA GTACGCATCT TCCGAGCAGA ACGCAGCTAC 
GCTGTTCAGA GCGGTCGCTG GTATTTTGAG TTTGAGGCCG TGACCACTGG AGAAATGCGT 
GTGGGCTGGG CCCGTCCAGA GCTGCGGCCA GACGTGGAGC TGGGAGCAGA TGATTTGGCT 
TATGTCTTCA ATGGCCACCG AGGGCAGCGC TGGCACATGG GCAGTGAGCC ATTTGGACGA 
CCCTGGCAAC CAGGCGACGT GGTTGGGTGT ATGATCGACC TTTCGGAGAA CAACATCCTC 
TTCACACTCA ATGGAGAAGT GCTCATGTCT GACTCGGGCT CGGAGACGGC CTTTAAGGAT 
ATTGAGATTG GGGATGGCTT CCTCCCTGTC TGCAGCCTGG GCCTGGGCCA GGTGGGACGC 
CTAAATTTGG GCCAGGATGT GGGATCTCTT CGCTATTTCA CCATCTGTGG ACTTCAGGAG 
GGCTTTGAAC CTTTTGCCAT CAACATGCAG CGGCCTGTCA CCACCTGGTT CAGCAAGAGC 
CTGCCCCAGT TTGAACCGAT GCCCCCTGAC CATCCCCACT ATGAGGTGAC CCGAGTGGAC 
GGCACGGTGG ACACGCCTCC CTGCCTGCGC CTGACGCACC GTACCTGGGG CTCCCAGAAC 
AGCCTGGTGG AGATGATGTT CCTGAGGCTG AGCCTCCCGG TGCAGTTCTA TCAGCACTTC 
CGCTGCACCC CGGGGGCCAC CCCCTTGGCC CCGCCGGGTC CCCAGCCCCC CGGGGACGAC 
GAGGTCCGAG CGGCCGAGCC TGACCCCGAC TACGAGCAGA TGCAGCGTTC GGCGGGCCGG 
ACGGGCGAGG CTGAGGGGAA CAAGGAGGGG ACTGGGGGGC CCACCAAGGA CGGGGCGGCT 
TCTCAGGCAG GAGCCGAAAC AACAGGAGGG TCCCCGACCC GAGCTGAGAA TGAGAAGGAC 
GCTGCCACTG AGAAAAGCAA GAAGAGGGGG TTCTTGTTCA AAGCCAAGAA GGCAGCCATG 
ATGACGCAAC CACCCGCCAC CCCCGCTCTG CCCCGACTCC CTCACGACGT GGTGTCCGCG 
GACAACAGGG ATGACCCTGA GATTATCCTC AACACCACCA CGTATTACTA CTCTGTCCGG 
GTGTTTGCCG GCCAGGAGCC CAGCTCAGTG TGGGTGGGCT GGGTCACCCC TGACTACCAC 
CAGCACGACA TGAATTTTGA TCTGACCAAA GTCCGCGTTG TGACGGTGAC CATGGGGGAC 
GAGCGAGGCA ATGTACACAA CAGCTTGAAG CGCAGTAACT GCTACATGGT GTGGGGTGGG 
GATTTTGTGA GCCCGGGACA GCAAGGACGC ATCAGTCACA CAGACCTGGT CATCGGCTGC 
CTGGTGGACC TGGCCACCGG CCTCATGACC TTCACGGCCA ATGGAAAAGA GAGCAACACC 
TTTTTCCAGG TGGAGCCAAA CACGAAGCTG TTTCCAGCGG CCTTCGTCCT TCCCACCAAC 
CAAAATGTTA TGCAATTTGA ACTGGGAAAG CTGAAGAACA TCATGCCCCT GTCAGCAGCC 
ATGTTCCTGA GTGAACGGAA GAACCCAGCG CCGCAGTGTC CCCCCCGCTT GGAGGTACAA 
ATGCTCATGC CCGTGTCCTG GAGCAGGATG CCCAACCACT TCCTACAAGT GGAGACACAC 
CGGGCAGGTG ACCGCCTGGG CTGGGCTGTG CAGTGTGAGG AGCCTCTCAC CATGATGGCC 
TTGCATATCC CTGAGGAGAA CCGGGGAGGC CATTGGCCTA CGATGGTGGG AGCCTCGACT 
TCCACACCGA GGGACCTGGA ACTGGTCCTG CAGGTGATGG GCATCTTCGG GGACGAGGAC 
GTCAAGCAAA TCCTGAGGAT GATTGAGCCT GAAGTTTTCA AGGAAGAGGA AGAGGAGGAA 
GAGGAGGAGG AGGAGGAGGA GGATGAAGAA GAGAAGGAAG AGGATGAAGA AGAAGAGGAA 
GAAGAAAAAG AGGAGGAGGT GAAAGAGGAG GAGGAGGAAG AGGAAGAGAA GGGAGAGGAA 
GAAGAAGCTG TGGAGGAAGG ACTACTTCAG ATGAAGCTGC CTGAGTCTGT CAAGCTGCAG 
ATGTGCCATC TGCTGGAGTA CTTCTGTGAC CAGGATCTGC AGCACCGAGT GGAGGCGCTC 
ATTGCGTTCT CGGAGCGCTA CGTCAGTTGC CTGCAGAACA ACCAGCGCAG CCGCTACGGG 
CTGCTCATAA AGGCCTTCAC TATGTCCGCA GCGGAGACTG CCCGGCGCAC CCGGGAGTTT 
CGCTCTCCGC CCCAGGAGCA GATCAACATG CTTCTGCACT TCAAAGGGAG TGAAGACGAG 
GAGGACTGTC CGGTCCCAGA CGAAATTCGG CAGGAGCTGC TGGATTTTCA CCAGGACCTG 
CTGCTGCACT GCGGCATCCA GCTGGAAACG GAGGAGGAGG AGGTGGAAGA GGAGACCACC 
CTGGGAGGCC GGCTCATGAG CTTTTTGGAG AAGGTGAAGC TGGTGAAGAA GAAGGAGAAG 
GAGCCAGATG AGACCCCTCC CCCAGAAGAG AAAAAGCCTG AGTCCTTGCA GGAGCTGGTC 
TCTCACACAA TGGTGCGCTG GGCCCAAGAG GACTTTATCC AGAACCCAGA GCTGGTCCGG 
GCCATGTTCA GCCTCCTCCA CCGGCAGTAC GATGGCCTCG GGGAGCTGCT CCGTGCCCTG 
CCCAGGGCTT ACACCATCTC CCCGTCCTCC GTGGAGGACA CCATGAGCCT CCTGGAAAGC 
CTGTGTCAGA TCCGCTCCTT GCTCATTGTG CAGATGGGGC CGCAAGAGGA GAATCTCATG 
ATCCAGAGCA TTGGGAATAT CATGAACAAC AAGGTGTTTT ACCAGCACCC AAATCTGATG 
AGGGCTTTGG GGATGCACGA AACAGTGATG GAAGTTATGG TCAATGTGTT AGGAGGGGGA 
GAGTCTAAGG AAATCCGCTT CCCCAAAATG GTCACCAGCT GCTGCCGGTT CCTCTGCTAT 
TTCTGTCGCA TTAGTCGCCA GAACCAAAGG TCCATGTTTG ATCACCTCAG CTACCTGCTG 
GAGAACAGCG GGATCGGCCT GGGTATGCAG GGTTCCACTC CGCTAGATGT GGCGGCTGCC 
TCCGTCATAG ACAACAACGA GCTGGCCTTG GCCCTTCAGG AACAAGACCT GGAAAAGGTC 
GTCTCCTACT TGGCCGGCTG CGGCCTTCAG AGCTGCCCCA TGCTGCTGGC CAAGGGCTAC 
CCCGACATTG GCTGGAACCC CTGCGGGGGG GAGCGCTATC TGGATTTCCT GAGATTTGCC 
GTCTTTGTTA ATGGAGAGAG CGTGGAAGAG AATGCGAATG TTGTGGTTCG TCTGCTCATC 
CGGCGTCCCG AATGCTTCGG CCCGGCCCTG CGAGGAGAGG GCGGCTCTGG GCTGCTGGCT 
GCTATGGAAG AGGCAATCCG GATCTCAGAG GATCCTGCTC GAGATGGCCC GGGCGTCAGG 
AAGGACCGGA GGCGGGAGCA CCACGGGGGT GAAGAGGCCC CTGAAGAAAA CCGTGTGCAC 
TTGGGAAATG CCATCATGTC ATTCTATTCC GCCCTGATCG ACCTGCTGGG ACGCTGTGCT 
CCAGAGACTC ATCTGATCCA GGCCGGGAAG GGAGAAGCGC TGAGGATCCG AGCCATTCTG 
CGCTCCCTGG TGCCCCTGGA CGACCTCGTG GGCATTATCA GCCTGCCCCT GCAGATCCCC 
ACCCTGGGCA AAGATGGGGC CCTGGTGCAG CCTCGGATGT CTGCCTCCTT CGTGCCTGAC 
CACAAAGCCC CCATGGTCCT CTTCCTGGAC CGCGTCTACG GCATCGACAG CCAGGACTTC 
CTGCTCCACG TGCTTGATGT CGGCTTCCTG CCTGACATGC GGGCGGCTGC CTCCCTGGAC 
ACGGCAACCT TCAGCACCAC GGAGATGGCC CTGGCCTTGA ACCGCTACCT GTGCACAGCC 
GTGCTTCCCC TGATCACCAA GTGCGCCCCA CTGTTTGCGG GGACGGAGCA CCGCGCCATC 
ATGGTGGACT CCATGCTCCA CACGGTGTAC CGCCTGTCCC GGGGACGCTC TCTCACCAAG 
GCCCAGCGAG ATGTCATTGA GGACTGCCTC ATGGCGCTCT GCAGGTACAT CCGTCCATCT 
ATGCTCCAGC ACCTTCTGCG TCGTCTGGTA TTCGACGTTC CCATCCTCAA CGAGTTTGCC 
AAGATGCCCC TCAAGCTCCT GACCAATCAC TATGAGCGCT GCTGGAAATA TTACTGCCTC 
CCTACGGGCT GGGCCAACTA TGGAGTTACC TCCGAGGAAG AGCTGCATCT GACTCGCAAG 
CTTTTCTGGG GCATCTTTGA GTCACTGGCC CACAAGAAAT ACGACCAGGA GCTCTACCGC 
ATGGCCATGC CGTGTCTGTG CGCCATCGCC GGGGCCCTGC CCCCCGACTA CGTGGATGCC 
TCCTACTCCT CCAAAGCTGA GAAGAAGGCC ACTGTGGATG CTGAGGGCAA CTTTGACCCC 
CGGCCCGTGG AGACACTCAA TGTGATCATA CCCGAGAAAC TCGACTCCTT CATCAACAAA 
TTTGCCGAGT ACACACACGA GAAGTGGGCA TTTGACAAGA TTCAGAACAA TTGGTCATTT 
GGGGAGAACA TTGACGAGGA GCTCAAGACC CACCACATGC TTCGGCCCTA CAAGACCTTC 
TCAGAAAAGG ACAAGGAGAT TTACCGATGG CCCATCAAGG AGTCCCTGAA GGCCATGATC 
GCCTGGGAGT GGACTGTGGA AAAGGCCCGT GAGGGTGAGG AGGAGAAGGG AGAGAAGAAG 
ACCAAGACTC GCAAGATCTC CCAGAATACC CCGGCCACCT ATGACCCAAG AGAGGGCTAT 
AATCCCCAGC CTCCAGACCT TACCTGTGTC ACCCTGTCCC GGGAACTGCA GGCCATGGCT 
GAACAACTGG CTGAGAATTA CCATAACACA TGGGGGAGGA AGAAAAAGCA GGAATTAGAA 
GCCAAGGGTG GAGGCACCCA CCCCCTCCTG GTCCCCTATG ACACACTGAC GGCCAAGGAG 
AAGGCTCGCG ATAGAGAGAA AGCCCAGGAA CTGCTCAAAT TCCTGCAGAT GAACGGCTAC 
GCGGTGACGC GGTGCCCCCA GGCCTTCCTC CATCTCCCCC GAGCCCTGTC CTGTTCCCCC 
AGGACCGTGA TGAAGTCTGG TCCCGAGATC GTCAAGGCTG GTCTCCGATC GTTCTTCGAG 
AGCGCTTCGG AGGACATCGA GAAGATGGTG GAGAACCTGC GCCTGGGCAA GGTGTCCCAG 
GCGCGGACGC AGGTCAAGGG CGTCGGCCAG AACCTCACCT ACACCACCGT GGCTCTGCTT 
CCCGTTCTCA CCACGCTTTT CCAGCACATC GCCCAGCACC AGTTTGGGGA TGATGTCATC 
TTGGACGACG TCCAGGTCTC CTGCTACCGA ACACTTTGCA GCATCTACTC TCTGGGGACC 
ACCAAGAATT CCTATGTGGA GAAGCTCCGT CCAGCCCTTG GAGAATGCCT TGCCCGTCTG 
GCAGCTGCTA TGCCAGTTGC CTTCCTTGAA CCCCAACTGA ATGAGTTCAA CGCCTGCTCT 
GTCTACACCA CCAAATCTCC CCGAGAACGT GCCATCCTGG GCCTTCCGAG CTCCGTGGAG 
GAGATGTGCC CAGATATCCC AGACCTGAAG CACCTGATTG CCGAGATCGG GGGGCGGGCC 
GAGTCGGGCG CCCGTTACAC CGAGATGCCC CATGTCATCG AGATCACGCT GCCGATGCTC 
TGTAACTACC TGCCCCGCTG GTGGGAGCGG GGGCCCGAGG TCCCCTCCTC ACAGCCCCCA 
GGGGGACCCC CACCCTGCAC AGATGTCACC TCGGACCACC TCAACTCCCT CCTGGGAAAC 
ATCCTCCGGA TCATTGTCAA CAACCTGGGC ATTGACGAGG CTTCGTGGAT GAAGCGCTTG 
GCAGTGTTTG CGCAGCCGAT CGTGAGCCGG GCCCGGCCCG AGCTGCTCCG CTCACATTTC 
ATCCCCACGG TGGGCCGGCT CCGGAAGCGC GCAGGGAAGG TGGTGGCCGA GGAAGAGCAG 
CTGAGGCTGG AAGCCAAGGC GGAGGCTGAG GAGGGAGAGC TCCTGGTGCG GGACGAGTTC 
TCTGTGCTCT GCCGGGATCT CTACGCACTC TACCCCCTGC TCATCCGCTA CGTAGACAAC 
AACCGAGCCC ACTGGCTAAC AGAACCCAAC CCTGATGCAG AAGAACTCTT CAGGATGATA 
GCAGAAATCT TCATCTATTG GTCCAAGTCT CATAACTTCA AGAGAGAAGA ACAGAATTTC 
GTGGTACAGA ACGAAATCAA TAACATGTCC TTCCTGACGG CAGATAGCAA GAGCAAGATG 
GCCAAGTCAG GAGGCTCCGA TCAAGAGAGA ACAAAGAAGA AACGGCGAGG GGACCGATAT 
TCCGTCCAGA CCTCTCTGAT CGTGGCTACC CTCAAGAAGA TGCTGCCTAT TGGGTTGAAC 
ATGTGTGCAC CCACTGACCA GGACCTCATC GCGCTGGCCA AGACTCGTTA TGCCCTGAAA 
GACACAGATG AAGAAGTTCG GGAATTTCTG CAGAATAACC TTCATCTTCA GGGAAAGAGT 
GAGGGCTCCC CATCACTGCG CTGGCAGATG GCCCTGTACC GGGGGCTGCC TGGCCGGGCT 
GAGGATGCTG ATGCCCCTGA GAACATTGTT CGCCGAGTAC AGGAAGTCTC AGCGGTGCTT 
TACCACCTGG AACAGACAGA ACATCCCTAC AAGTCCAAGA AAGCTGTGTG GCACAAGCTG 
CTTTCAAAAC AGCGTCGTCG GGCGGTTGTG GCTTGTTTTC GCATGACACC CTTGTACAAC 
TTGCCTCGGC ATCGGGCAAG CAATATGTTC TTGGAGTCCT ACAAAGCATC ATGGATCCTA 
ACTGAGGAGC ACAGTTTTGA GGACCGCATG ATTGATGACC TTTCTAAAGC TGGGGAGGAA 
GAAGAAGAGG AAGAAGAACA GGAAGAGAAG AAGCCAGACC CTCTTCACCA GCTGGTATTA 
CACTTTAGCC GGACAGCCCT GACAGAGAAG AGCAAACTAG ATGAGGACTA TTTGTACATG 
GCATATGCTG ACATCATGGC CAAGAGCTGC CACTTGGAGG AGGAAGAGGA GCCTTCAGAA 
CCTGCTGAGG AGGAGGCTGA AGTTTCATTT GAGGAGAAGG AGATGGAAAA GCAGAAACTG 
CTCTACCAGC AGTCAAGGCT ACACAACCGA GGGGCTGCTG AGATGGTGCT ACAAATGATT 
AGTGCCTGCA AAGGGGAGCC GGGAGTCATG GTTTCATCCA CCCTGAAGTT GGGCATCTCC 
ATCCTCAACG GTGGCAACAT GGAGGTGCAG CAGAAGATGC TGGACTATCT GAAGGACAAA 
AAGGATGTTG GCTTCTTCCA GAGCATCCAG GCCCTGATGC AGACATGCAG TGTTCTTGAT 
CTGAATGCCT TTGAGAGGCA GAACAAGGCA GAGGGGCTTG GAATGGTGAC CGAGGAAGGG 
ACTGGGGAAA AAGTAATGGC CGACGATGAG TTCACCCAGG ATCTCTTCCG CTTTCTGCAG 
CTGCTGTGTG AAGGCCATAA CAATGACTTT CAGAACTACC TCCGCACGCA GACGGGGAAC 
ACGACCACCA TCAACATCAT CATCTGCACA GTGGATTATC TGCTGAGGCT GCAGGAGTCC 
ATCAGTGACT TCTACTGGTA CTACTCGGGG AAGGATGTGA TCGAAGAGCA AGGGAAGAGG 
AACTTCTCCA AAGCCATGGC CGTGGCCAAG CAGGTCTTCA ACAGCCTCAC CGAGTACATC 
CAGGGCCCGT GCACAGGAAA CCAGCAGAGC CTGGCCCACA GCCGCCTGTG GGATGCCGTG 
GTCGGCTTCC TCCACGTTTT TGCCCACATG ATGATGAAGC TGGCTCAGGA CTCAAGCCAG 
ATTGAGCTGC TGAAGGAGCT CCTGGATCTG CAGAAAGACA TGGTGGTCAT GTTGCTTTCG 
CTGCTGGAAG GTAACGTGGT CAACGGCACA ATTGCCCGGC AGATGGTGGA CATGCTGGTG 
GAGTCCTCCT CCAACGTGGA GATGATCCTC AAGTTCTTCG ACATGTTCCT AAAGCTGAAG 
GACATCGTGG GCTCCGAGGC ATTCCAGGAC TACGTCACCG ACCCCCGTGG ACTCATCTCC 
AAGAAGGATT TCCAGAAGGC CATGGACAGC CAGAAGCAGT TCACTGGCCC AGAGATCCAG 
TTCCTCCTGT CCTGCTCAGA GGCGGATGAG AACGAGATGA TCAATGTGGA GGAATTTGCC 
AGCCGCTTCC AGGAGCCGGC CCGAGACATC GGCTTCAACG TGGCCGTGCT GCTGACCAAC 
CTGTCGGAGC ACGTGCCCCA CGACCCGCGC CTCAAGAACT TCCTGGAGCT GGCTGAGAGC 
ATCCTGGAGT ACTTCCGGCC CTACCTGGGC CGGATCGAGA TCATGGGGGC CTCCCGGCGC 
ATCGAGCGCA TCTACTTTGA AATCTCTGAG ACCAACAAAG CACAGTGGGA GATGCCCCAG 
GTGAAGGAGT CCAAACGCCA GTTCATCTTT GACGTGGTGA ATGAAGGGGG GGAGTCGGAG 
AAGATGGAAA TGTTTGTGAG CTTCTGTGAA GACACCATCT TTGAGATGCA GATCGCCGCC 
CAGATCTCAG AGCCCGAGGG AGAGCCTGAG GAAGATGAGG AGGAGTCCAT GGCAGAGTCG 
GGGCCAGACT CCGGGGAGGC AGGGCCCGAC GGAGAAGGGG CCGGCGCAGG GGGCCCGACG 
GGCATCTCCG GGCACGTGGC CGGTGCCGTC TCCTCGGTCC TGCGGAAGCT GACCTACCGG 
AGCCTGCGCA GGAGGGTGCG GAGGCTCCGC AAGCTGACCC TGCGGGAGGC TGCAGGAACC 
GGGGCCGGGC TCATCTGGGC CGGGGCCACG TGGACGGGCT CCGGAGTGAT CGGCGCCACC 
CGGGGGGCCC TGGGCCTACT CTGGGGCTCT CTGTTTGGGG GAGGGCTGGT GGAGGGAGCC 
AAGAAAGTGA CCGTGACGGG ACTGTTGGCC GGGATGCCAG ACCCCACAGG CGATGAGGTC 
CATGGGGAGC AGGCGGCTGG CGCCGGGGCT GCCGGGACTG GGGACGGAGC CGTGGAGGGT 
GATGAGGAGG TGGGGGCGGA GTTGGCCGAG AGGGAGGCTG AGGCTGAGGC CGGAGCTGGG 
GAGGCCGGGC CAGCCGGAGG AGAGGGAGCC GGGGCCAATG GAGGCCACTT CCGGATGGAG 
GTCCCCGGAG GCCTGGGTGA CATGGGAGAC ACAACACCCG TTGAGCCGCC CACTCCTGAG 
GGTTCCCCCA TCTTCCGGAG GAAACTAGGG CTGGAGGGAG AAGAAGAACA AGCCTTAGAA 
CCAGAACCAG AGCCAGAACC AGAACCAGAG CCAGAGCCAG AACCCGAGCC TGAGAAAGCT 
GATGCTGAGA ACGGAGAAAA GGAAGGGTCC CAAGAGCCAG AACCTGAGGC TCCCAAGAAG 
GCAGCTGCCC CACCTCCGCC CCGGAAGGAG GACGCCAAAT CTGAGGGCCC TGACTTCTGG 
GAGGAAATGG AGGTGCAGAG GGTCAAGTTC CTGAACTACC TCTCCAGAAA CTTCTATACC 
CTGAGATTCC TCGCCCTCTT CCTGGCCTTT GCCATCAACT TCATCCTGCT CTTCTACAAG 
GTGTCTGACT CTCCGCCAGG AGAAGAGGAC CTGGAAGGCT CCGGTCTCGA GGGTCTGGCA 
GGCTCAGGGT CCGGGGACTT CCTGGGATCC GGCATGGGCT CCGGGGCTGG GGAGGATCTG 
GAGAACGAGG AGGAAGAGAA CATGGTCTAC TACTTCCTGG AAGAGAGTAC GGGCTACATG 
GAGCCAGCCC TGCGAGGTCT CAGCATCCTG CACACCCTCA TTGCCTTCCT CTGCATCATA 
GGCTACAACT GTCTCAAGGT ACCGCTGGTT ATATTTAAAC GGGAGAAGGA GCTAGCCCGG 
AAACTAGAGT TCGATGGCCT TTACATCACT GAGCAGCCCG AGGATGACGA CGTGAAGGGC 
CAGTGGGATC GACTGGTGCT TAACACCCCG TCCTTCCCCA GCAACTACTG GGACAAGTTC 
GTGAAAAGGA AGGTGCTGGA GAAACATGGC GACATCTTTG GCCGGGAGCG GATTGCAGAG 
CTGTTGGGCA TGGATCTGGC CAGCCTGGAG ATCACGGCGC AGAACGAGAA GCGACCGGAA 
CCCCCCTCGG GACTGCTCAC CTGGATCATG TCCATAGATG TCAAATACCA AATCTGGAAA 
TTTGGTGTCA TCTTTACAGA TAATTCATTC TTGTATTTGA CGTGGTACAT GGTCATGTCC 
CTCCTCGGCC ACTATAACAA CTTCTTCTTT GCCGCTCACC TGCTGGACAT CGCCATGGGA 
GTCAAGACGC TTCGGACCAT CCTGTCTTCT GTCACCCACA ATGGGAAGCA GCTGGTGATG 
ACGGTGGGCC TGCTGGCCGT CGTGGTTTAC CTCTACACGG TTGTGGCCTT CAATTTCTTC 
CGCAAATTTT ACAATAAAAG CGAGGATGAG GATGAGCCGG ACATGAAATG TGATGACATG 
ATGACGTGTT ACCTGTTCCA CATGTACGTG GGGGTCCGAG CTGGTGGGGG CATCGGGGAT 
GAGATTGAGG ATCCAGCAGG GGATGAATAC GAGCTCTACC GTGTGGTCTT TGACATCACC 
TTTTTCTTCT TCGTCATCGT CATCCTGCTG GCCATTATCC AAGGTCTGAT CATTGATGCT 
TTTGGGGAGC TCCGAGACCA GCAAGAGCAA GTGAAGGAAG ATATGGAGAC CAAATGTTTC 
ATCTGTGGCA TTGGAAGTGA CTACTTTGAT ACAACACCCC ACGGTTTTGA GACCCACACG 
CTGGAGGAGC ACAACCTGGC TAACTACATG TTTTTCCTGA TGTATCTGAT CAATAAGGAT 
GAGACCGAGC ACACGGGACA GGAGTCGTAT GTCTGGAAGA TGTACCAGGA GCGCTGCTGG 
GACTTCTTCC CAGCTGGTGA CTGTTTCCGA AAACAGTATG AAGACCAGCT GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RYR1 ( XM_031961617.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031817477.1 Sarcophilus harrisii ryanodine receptor 1 (RYR1), mRNA.

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CloneID	OSa230812
Clone ID Related Accession (Same CDS sequence)	XM_031961617.1
Accession Version	XM_031961617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	LOW QUALITY PROTEIN: ryanodine receptor 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases frameshifts :: corrected 2 indels internal stop codons :: corrected 2 genomic stop codons ##RefSeq-Attributes-END##

ATGGCGGACG TCCTGGATAG GATGGTACTG CGGTGGCTTC CGCTCGTACG GGGTACCAAT 
GCGGGCGGGT TAGCCCTGAA TGACGGCCGG TGGTGCGGTA TGCGGTCCGT GGACCGGGCG 
GTGGCAGCGC GTCATCGGGT TGGCGCGCGG CGGGCCATCG GTTCTCTGAC CTGTCGTCGG 
TCCTGGATGG CGACGCTCGC GCGCTCCCAT GCTCAGCAGG TACGCCATTG CTGGCGCCCG 
AGACCAGCAC CATCACCCTG TTCCCGCGGA GCTGTCGCCC GGCGAGGCTT CATGGCGCGG 
GCTGCCGAGT GGGCGCCTCC CGTCCTCACG CCCGCCCTAC ACTTCGCCGC CCTGCCCATT 
GGCAGCGCCC AGCGCCCGGG AGGCCAGCAC GCCCGCGACC CCGTGGGGGG CTCTGTGGAG 
TTCCAGTTCG TTCCGGTGCT CAAACTGGTG TCCACCCTGC TGGTAACCAC CGCGTCCCTC 
CACCCACCCT TCCTCCCCCC ACTCTGCCCT CAGGCTTCAG TCTCCAACTC TGTCCGCCAA 
TCGTTCCCTT CATCCGGCAG TCACTCGGTC ATCCGTCAGT CATTGGTTCA TCCGTCAGTC 
ATTGGTTCAT CCGTCAGTTG CCCACCCCTG AACTTGGGCA CTTCCCCCGT CCGTCAGTCC 
CCCCTCCCCC ATCCGTCAGT CCCCTACGAA ATCCGCTTCC CCAAAATGGT CACCAGCTGC 
TGCCGGTTCC TCTGCTATTT CTGTCGCATT AGTCGCCAGA ACCAAAGGTC CATGTTTGAT 
CACCTCAGCT ACCTGCTGGA GAACAGCGGG ATCGGCCTGG GTATGCAGGG TTCCACTCCG 
CTAGATGTGG CGGCTGCCTC CGTCATAGAC AACAACGAGC TGGCCTTGGC CCTTCAGGAA 
CAAGACCTGG AAAAGGTCGT CTCCTACTTG GCCGGCTGCG GCCTTCAGAG CTGCCCCATG 
CTGCTGGCCA AGGGCTACCC CGACATTGGC TGGAACCCCT GCGGGGGGGA GCGCTATCTG 
GATTTCCTGA GATTTGCCGT CTTTGTTAAT GGAGAGAGCG TGGAAGAGAA TGCGAATGTT 
GTGGTTCGTC TGCTCATCCG GCGTCCCGAA TGCTTCGGCC CGGCCCTGCG AGGAGAGGGC 
GGCTCTGGGC TGCTGGCTGC TATGGAAGAG GCAATCCGGA TCTCAGAGGA TCCTGCTCGA 
GATGGCCCGG GCGTCAGGAA GGACCGGAGG CGGGAGCACC ACGGGGGTGA AGAGGCCCCT 
GAAGAAAACC GTGTGCACTT GGGAAATGCC ATCATGTCAT TCTATTCCGC CCTGATCGAC 
CTGCTGGGAC GCTGTGCTCC AGAGACTCAT CTGATCCAGG CCGGGAAGGG AGAAGCGCTG 
AGGATCCGAG CCATTCTGCG CTCCCTGGTG CCCCTGGACG ACCTCGTGGG CATTATCAGC 
CTGCCCCTGC AGATCCCCAC CCTGGGCAAA GATGGGGCCC TGGTGCAGCC TCGGATGTCT 
GCCTCCTTCG TGCCTGACCA CAAAGCCCCC ATGGTCCTCT TCCTGGACCG CGTCTACGGC 
ATCGACAGCC AGGACTTCCT GCTCCACGTG CTTGATGCGG GTTCCCCTGG CGGGCGGTGC 
CTCCCTGGAC CGGGGGGCGC AACCTTTCCA AAGGAGATGG CCCTGGCCTT GAACCCTACT 
TGCACACCTG TTTATCCCCA AGTCCCCCCA CTGTTTGGGN GAAGGGCCGC CATCATGGTG 
GACTCCATGC TCCACACGGT GTACCGCCTG TCCCGGGGAC GCTCTCTCAC CAAGGCCCAG 
CGAGATGTCA TTGAGGACTG CCTCATGGCG CTCTGCAGGT ACATCCGTCC ATCTATGCTC 
CAGCACCTTC TGCGTCGTCT GGTATTCGAC GTTCCCATCC TCAACGAGTT TGCCAAGATG 
CCCCTCAAGC TCCTGACCAA TCACTATGAG CGCTGCTGGA AATATTACTG CCTCCCTACG 
GGCTGGGCCA ACTATGGAGT TACCTCCGAG GAAGAGCTGC ATCTGACTCG CAAGCTTTTC 
TGGGGCATCT TTGAGTCACT GGCCCACAAG AAATACGACC AGGAGCTCTA CCGCATGGCC 
ATGCCGTGTC TGTGCGCCAT CGCCGGGGCC CTGCCCCCCG ACTACGTGGA TGCCTCCTAC 
TCCTCCAAAG CTGAGAAGAA GGCCACTGTG GATGCTGAGG GCAACTTTGA CCCCCGGCCC 
GTGGAGACAC TCAATGTGAT CATACCCGAG AAACTCGACT CCTTCATCAA CAAATTTGCC 
GAGTACACAC ACGAGAAGTG GGCATTTGAC AAGATTCAGA ACAATTGGTC ATTTGGGGAG 
AACATTGACG AGGAGCTCAA GACCCACCAC ATGCTTCGGC CCTACAAGAC CTTCTCAGAA 
AAGGACAAGG AGATTTACCG ATGGCCCATC AAGGAGTCCC TGAAGGCCAT GATCGCCTGG 
GAGTGGACTG TGGAAAAGGC CCGTGAGGGT GAGGAGGAGA AGGGAGAGAA GAAGACCAAG 
ACTCGCAAGA TCTCCCAGAA TACCCCGGCC ACCTATGACC CAAGAGAGGG CTATAATCCC 
CAGCCTCCAG ACCTTACCTG TGTCACCCTG TCCCGGGAAC TGCAGGCCAT GGCTGAACAA 
CTGGCTGAGA ATTACCATAA CACATGGGGG AGGAAGAAAA AGCAGGAATT AGAAGCCAAG 
GGTGGAGGCA CCCACCCCCT CCTGGTCCCC TATGACACAC TGACGGCCAA GGAGAAGGCT 
CGCGATAGAG AGAAAGCCCA GGAACTGCTC AAATTCCTGC AGATGAACGG CTACGCGGTG 
ACGCGAGGGC TGAAGGACAT GGAGCTGGAC ACATCTTCAA TTGAGAAGAG GTTTGCGTTT 
GGGTTCCTCC AGCAGCTGCT CCGATGGATG GACATCTCAC AGGAGTTCAT TGCCCATCTG 
GAGGCTGTGG TGAGCAGCGG ACGGGTGGAG AAGTCTCCTC ACGAGCAAGA GATCAAGTTC 
TTTGCCAAGA TCCTCCTGCC GCTCATCAAC CAGTACTTCA ACAACCACTG CCTCTACTTT 
CTGTCCACAC CAGCCAAGGT GCTCGGCAGT GGGGGCCACG CCTCTAACAA GGAAAAGGAG 
ATGATCACCA GCCTGTTCTG TAAACTCGCC GCTCTGGTCC GCCACCGAGT CTCTCTCTTT 
GGGACTGACG CTCCAGCTGT GGTCAACTGC CTGCACATCC TCGCCCGGTC CCTGGATGCA 
AGGACCCACC AGTTTGGGGA TGATGTCATC TTGGACGACG TCCAGGTCTC CTGCTACCGA 
ACACTTTGCA GCATCTACTC TCTGGGGACC ACCAAGAATT CCTATGTGGA GAAGCTCCGT 
CCAGCCCTTG GAGAATGCCT TGCCCGTCTG GCAGCTGCTA TGCCAGTTGC CTTCCTTGAA 
CCCCAACTGA ATGAGTTCAA CGCCTGCTCT GTCTACACCA CCAAATCTCC CCGAGAACGT 
GCCATCCTGG GCCTTCCGAG CTCCGTGGAG GAGATGTGCC CAGATATCCC AGACCTGAAG 
CACCTGATGG CCGAGATCGG GGGGCTGGCC GAGTCGGGCG CCCGTCACAC CGAGATGCCC 
CATGTCATCG AGATCACGCT GCCGATGCTC TGTAACTACC TGCCCCGCTG GTGGGAGCGG 
GGGCCCGAGG TCCCCTCCTC ACAGCCCCCA GGGGGACCCC CACCCTGCAC ANNGATGCAC 
CCGGACCACC CCTAATCCCT CCTAAACATC CTCCGATTTG TCAACAACCT GGGATAAGGG 
GTTTTCGTGG ATGGCGCTCA GGGGGACCGG AATGAAGCGC TTGGGGTGTG GATGTGGAGG 
CTCCCCAGGC CCACCAACGC CCCTGCCTCG TGTCCCGCAG TGTTTGCGCA GCCGATCGTG 
AGCCGGGCCC GGCCCGAGCT GCTCCGCTCA CATTTCATCC CCACGGTGGG CCGGCTCCGG 
AAGCGCGCAG GGAAGGTGGT GGCCGAGGAA GAGCAGCTGA GGCTGGAAGC CAAGGCGGAG 
GCTGAGGAGG GAGAGCTCCT GGTGCGGGAC GAGTTCTCTG TGCTCTGCCG GGATCTCTAC 
GCACTCTACC CCCTGCTCAT CCGCTACGTA GACAACAACC GAGCCCACTG GCTAACAGAA 
CCCAACCCTG ATGCAGAAGA ACTCTTCAGG ATGATAGCAG AAATCTTCAT CTATTGGTCC 
AAGTCTCATA ACTTCAAGAG AGAAGAACAG AATTTCGTGG TACAGAACGA AATCAATAAC 
ATGTCCTTCC TGACGGCAGA TAGCAAGAGC AAGATGGCCA AGGCGGGAGA TGGACAGTCA 
GGAGGCTCCG ATCAAGAGAG AACAAAGAAG AAACGGCGAG GGGACCGATA TTCCGTCCAG 
ACCTCTCTGA TCGTGGCTAC CCTCAAGAAG ATGCTGCCTA TTGGGTTGAA CATGTGTGCA 
CCCACTGACC AGGACCTCAT CGCGCTGGCC AAGACTCGTT ATGCCCTGAA AGACACAGAT 
GAAGAAGTTC GGGAATTTCT GCAGAATAAC CTTCATCTTC AGGGAAAGAG TGAGGGCTCC 
CCATCACTGC GCTGGCAGAT GGCCCTGTAC CGGGGGCTGC CTGGCCGGGC TGAGGATGCT 
GATGCCCCTG AGAACATTGT TCGCCGAGTA CAGGAAGTCT CAGCGGTGCT TTACCACCTG 
GAACAGACAG AACATCCCTA CAAGTCCAAG AAAGCTGTGT GGCACAAGCT GCTTTCAAAA 
CAGCGTCGTC GGGCGGTTGT GGCTTGTTTT CGCATGACAC CCTTGTACAA CTTGCCTCGG 
CATCGGGCAA GCAATATGTT CTTGGAGTCC TACAAAGCAT CATGGATCCT AACTGAGGAG 
CACAGTTTTG AGGACCGCAT GATTGATGAC CTTTCTAAAG CTGGGGAGGA AGAAGAAGAG 
GAAGAAGAAC AGGAAGAGAA GAAGCCAGAC CCTCTTCACC AGCTGGTATT ACACTTTAGC 
CGGACAGCCC TGACAGAGAA GAGCAAACTA GATGAGGACT ATTTGTACAT GGCATATGCT 
GACATCATGG CCAAGAGCTG CCACTTGGAG GAGGAAGAGG AGCCTTCAGA ACCTGCTGAG 
GAGGAGGCTG AAGTTTCATT TGAGGAGAAG GAGATGGAAA AGCAGAAACT GCTCTACCAG 
CAGTCAAGGC TACACAACCG AGGGGCTGCT GAGATGGTGC TACAAATGAT TAGTGCCTGC 
AAAGGGGAGC CGGGAGTCAT GGTTTCATCC ACCCTGAAGT TGGGCATCTC CATCCTCAAC 
GGTGGCAACA TGGAGGTGCA GCAGAAGATG CTGGACTATC TGAAGGACAA AAAGGATGTT 
GGCTTCTTCC AGAGCATCCA GGCCCTGATG CAGACATGCA GTGTTCTTGA TCTGAATGCC 
TTTGAGAGGC AGAACAAGGC AGAGGGGCTT GGAATGGTGA CCGAGGAAGG GACTGTCATC 
AATCGCCAGA ACGGGGAAAA AGTAATGGCC GACGATGAGT TCACCCAGGA TCTCTTCCGC 
TTTCTGCAGC TGCTGTGTGA AGGCCATAAC AATGGTTCGT ATGTGAGAGG AGAAAGAGGA 
GGGAGCACAC CCATCAACAT CATCATCTGC AGTGGATTAC TCTTGAGGGC TGGGGAGGGT 
CCATCAGGCT TTACCGGAAC TGGGGGAAAT GGACAAGACG GGAGGAACTT CTCCAAAGCC 
ATGGCCGTGG CCAAGCAGGT CTTCAACAGC CTCACCGAGT ACATCCAGGT AGTCACCTTG 
TCCCATCCGT GGCCCGGCCC CCAGTCCCTC CTGGCCTTCC AGGACTCAAG CCAGATTGAG 
CTGCTGAAGG AGCTCCTGGA TCTGCAGAAA GACATTGGGG TTTGGCTTTG CTGTGGAAGG 
TCCCCGCCAC CCAAACCACC CAGATCTCCC CAGGAGAAAT CCCAGGACCA GAGGAACCCT 
CATCCCGGCT GGAGCCTGAC AGACACGGCT ATGGTGCTCC TCACCGCTGA CGCTCCTCCC 
CTTACTCCCC GCCCCCCGGC AGGTAACGTG GTCAACGGCA CAATTGCCCG GCAGATGGTG 
GACATGCTGG TGGAGTCCTC CTCCAACGTG GAGATGATCC TCAAGTTCTT CGACATGTTC 
CTAAAGCTGA AGGACATCGT GGGCTCCGAG GCATTCCAGG ACTACGTCAC CGACCCCCGT 
GGACTCATCT CCAAGAAGGA TTTCCAGAAG GCCATGGACA GCCAGAAGCA GTTCACTGGC 
CCAGAGATCC AGTTCCTCCT GTCCTGCTCA GAGGCGGATG AGAACGAGAT GATCAATGTG 
GAGGAATTTG CCAGCCGCTT CCAGGAGCCG GCCCGAGACA TCGGCTTCAA CGTGGCCGTG 
CTGCTGACCA ACCTGTCGGA GCACGTGCCC CACGACCCGC GCCTCAAGAA CTTCCTGGAG 
CTGGCTGAGA GCATCCTGGA GTACTTCCGG CCCTACCTGG GCCGGATCGA GATCATGGGG 
GCCTCCCGGC GCATCGAGCG CATCTACTTT GAAATCTCTG AGACCAACAA AGCACAGTGG 
GAGATGCCCC AGGTGAAGGA GTCCAAACGC CAGTTCATCT TTGACGTGGT GAATGAAGGG 
GGGGAGTCGG AGAAGATGGA AATGTTTGTG AGCTTCTGTG AAGACACCAT CTTTGAGATG 
CAGATCGCCG CCCAGATCTC AGAGCCCGAG GGAGAGCCTG AGGAAGATGA GGAGGAGTCC 
ATGGCAGAGT CGGGGCCAGA CTCCGGGGAG GCAGGGCCCG ACGGAGAAGG GGCCGGCGCA 
GGGGGCCCGA CGGGCATCTC CGGGCACGTG GCCGGTGCCG TCTCCTCGGT CCTGCGGAAG 
CTGACCTACC GGAGCCTGCG CAGGAGGGTG CGGAGGCTCC GCAAGCTGAC CCTGCGGGAG 
GCTGCAGGAA CCGGGGCCGG GCTCATCTGG GCCGGGGCCA CGTGGACGGG CTCCGGAGTG 
ATCGGCGCCA CCCGGGGGGC CCTGGGCCTA CTCTGGGGCT CTCTGTTTGG GGGAGGGCTG 
GTGGAGGGAG CCAAGAAAGT GACCGTGACG GGACTGTTGG CCGGGATGCC AGACCCCACA 
GGCGATGAGG TCCATGGGGA GCAGGCGGCT GGCGCCGGGG CTGCCGGGAC TGGGGACGGA 
GCCGTGGAGG GTGATGAGGA GGTGGGGGCG GAGTTGGCCG AGAGGGAGGC TGAGGCTGAG 
GCCGGAGCTG GGGAGGCCGG GCCAGCCGGA GGAGAGGGAG CCGGGGCCAA TGGAGGCCAC 
TTCCGGATGG AGGTCCCCGG AGGCCTGGGT GACATGGGAG ACACAACACC CGTTGAGCCG 
CCCACTCCTG AGGGTTCCCC CATCTTCCGG AGGAAACTAG GGCTGGAGGG AGAAGAAGAA 
CAAGCCTTAG AACCAGAACC AGAGCCAGAA CCAGAACCAG AGCCAGAGCC AGAACCCGAG 
CCTGAGAAAG CTGATGCTGA GAACGGAGAA AAGGAAGGGT CCCAAGAGCC AGAACCTGAG 
GCTCCCAAGA AGGCAGCTGC CCCACCTCCG CCCCGGAAGG AGGACGCCAA ATCTGAGGGC 
CCTGACTTCT GGGAGGAAAT GGAGGTGCAG AGGGTCAAGT TCCTGAACTA CCTCTCCAGA 
AACTTCTATA CCCTGAGATT CCTCGCCCTC TTCCTGGCCT TTGCCATCAA CTTCATCCTG 
CTCTTCTACA AGGTGTCTGA CTCTCCGCCA GGAGAAGAGG ACCTGGAAGG CTCCGGTCTC 
GAGGGTCTGG CAGGCTCAGG GTCCGGGGAC TTCCTGGGAT CCGGCATGGG CTCCGGGGCT 
GGGGAGGATC TGGAGAACGA GGAGGAAGAG AACATGGTCT ACTACTTCCT GGAAGAGAGT 
ACGGGCTACA TGGAGCCAGC CCTGCGAGGT CTCAGCATCC TGCACACCCT CATTGCCTTC 
CTCTGCATCA TAGGCTACAA CTGTCTCAAG GTACCGCTGG TTATATTTAA ACGGGAGAAG 
GAGCTAGCCC GGAAACTAGA GTTCGATGGC CTTTACATCA CTGAGCAGCC CGAGGATGAC 
GACGTGAAGG GCCAGTGGGA TCGACTGGTG CTTAACACCC CGTCCTTCCC CAGCAACTAC 
TGGGACAAGT TCGTGAAAAG GAAGGTGCTG GAGAAACATG GCGACATCTT TGGCCGGGAG 
CGGATTGCAG AGCTGTTGGG CATGGATCTG GCCAGCCTGG AGATCACGGC GCAGAACGAG 
AAGCGACCGG AACCCCCCTC GGGACTGCTC ACCTGGATCA TGTCCATAGA TGTCAAATAC 
CAAATCTGGA AATTTGGTGT CATCTTTACA GATAATTCAT TCTTGTATTT GACGTGGTAC 
ATGGTCATGT CCCTCCTCGG CCACTATAAC AACTTCTTCT TTGCCGCTCA CCTGCTGGAC 
ATCGCCATGG GAGTCAAGAC GCTTCGGACC ATCCTGTCTT CTGTCACCCA CAATGGGAAG 
CAGCTGGTGA TGACGGTGGG CCTGCTGGCC GTCGTGGTTT ACCTCTACAC GGTTGTGGCC 
TTCAATTTCT TCCGCAAATT TTACAATAAA AGCGAGGATG AGGATGAGCC GGACATGAAA 
TGTGATGACA TGATGACGTG TTACCTGTTC CACATGTACG TGGGGGTCCG AGCTGGTGGG 
GGCATCGGGG ATGAGATTGA GGATCCAGCA GGGGATGAAT ACGAGCTCTA CCGTGTGGTC 
TTTGACATCA CCTTTTTCTT CTTCGTCATC GTCATCCTGC TGGCCATTAT CCAAGGTCTG 
ATCATTGATG CTTTTGGGGA GCTCCGAGAC CAGCAAGAGC AAGTGAAGGA AGATATGGAG 
ACCAAATGTT TCATCTGTGG CATTGGAAGT GACTACTTTG ATACAACACC CCACGGTTTT 
GAGACCCACA CGCTGGAGGA GCACAACCTG GCTAACTACA TGTTTTTCCT GATGTATCTG 
ATCAATAAGG ATGAGACCGA GCACACGGGA CAGGAGTCGT ATGTCTGGAA GATGTACCAG 
GAGCGCTGCT GGGACTTCTT CCCAGCTGGT GACTGTTTCC GAAAACAGTA TGAAGACCAG 
CTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031817477.1
CDS	1..9069
Translation	MADVLDRMVLRWLPLVRGTNAGGLALNDGRWCGMRSVDRAVAARHRVGARRAIGSLTCRRSWMATLARSHAQQVRHCWRPRPAPSPCSRGAVARRGFMARAAEWAPPVLTPALHFAALPIGSAQRPGGQHARDPVGGSVEFQFVPVLKLVSTLLVTTASLHPPFLPPLCPQASVSNSVRQSFPSSGSHSVIRQSLVHPSVIGSSVSCPPLNLGTSPVRQSPLPHPSVPYEIRFPKMVTSCCRFLCYFCRISRQNQRSMFDHLSYLLENSGIGLGMQGSTPLDVAAASVIDNNELALALQEQDLEKVVSYLAGCGLQSCPMLLAKGYPDIGWNPCGGERYLDFLRFAVFVNGESVEENANVVVRLLIRRPECFGPALRGEGGSGLLAAMEEAIRISEDPARDGPGVRKDRRREHHGGEEAPEENRVHLGNAIMSFYSALIDLLGRCAPETHLIQAGKGEALRIRAILRSLVPLDDLVGIISLPLQIPTLGKDGALVQPRMSASFVPDHKAPMVLFLDRVYGIDSQDFLLHVLDAGSPGGRCLPGPGGATFPKEMALALNPTCTPVYPQVPPLFGnullRAAIMVDSMLHTVYRLSRGRSLTKAQRDVIEDCLMALCRYIRPSMLQHLLRRLVFDVPILNEFAKMPLKLLTNHYERCWKYYCLPTGWANYGVTSEEELHLTRKLFWGIFESLAHKKYDQELYRMAMPCLCAIAGALPPDYVDASYSSKAEKKATVDAEGNFDPRPVETLNVIIPEKLDSFINKFAEYTHEKWAFDKIQNNWSFGENIDEELKTHHMLRPYKTFSEKDKEIYRWPIKESLKAMIAWEWTVEKAREGEEEKGEKKTKTRKISQNTPATYDPREGYNPQPPDLTCVTLSRELQAMAEQLAENYHNTWGRKKKQELEAKGGGTHPLLVPYDTLTAKEKARDREKAQELLKFLQMNGYAVTRGLKDMELDTSSIEKRFAFGFLQQLLRWMDISQEFIAHLEAVVSSGRVEKSPHEQEIKFFAKILLPLINQYFNNHCLYFLSTPAKVLGSGGHASNKEKEMITSLFCKLAALVRHRVSLFGTDAPAVVNCLHILARSLDARTHQFGDDVILDDVQVSCYRTLCSIYSLGTTKNSYVEKLRPALGECLARLAAAMPVAFLEPQLNEFNACSVYTTKSPRERAILGLPSSVEEMCPDIPDLKHLMAEIGGLAESGARHTEMPHVIEITLPMLCNYLPRWWERGPEVPSSQPPGGPPPCTnullMHPDHPSLLNILRFVNNLGGVFVDGAQGDRNEALGVWMWRLPRPTNAPASCPAVFAQPIVSRARPELLRSHFIPTVGRLRKRAGKVVAEEEQLRLEAKAEAEEGELLVRDEFSVLCRDLYALYPLLIRYVDNNRAHWLTEPNPDAEELFRMIAEIFIYWSKSHNFKREEQNFVVQNEINNMSFLTADSKSKMAKAGDGQSGGSDQERTKKKRRGDRYSVQTSLIVATLKKMLPIGLNMCAPTDQDLIALAKTRYALKDTDEEVREFLQNNLHLQGKSEGSPSLRWQMALYRGLPGRAEDADAPENIVRRVQEVSAVLYHLEQTEHPYKSKKAVWHKLLSKQRRRAVVACFRMTPLYNLPRHRASNMFLESYKASWILTEEHSFEDRMIDDLSKAGEEEEEEEEQEEKKPDPLHQLVLHFSRTALTEKSKLDEDYLYMAYADIMAKSCHLEEEEEPSEPAEEEAEVSFEEKEMEKQKLLYQQSRLHNRGAAEMVLQMISACKGEPGVMVSSTLKLGISILNGGNMEVQQKMLDYLKDKKDVGFFQSIQALMQTCSVLDLNAFERQNKAEGLGMVTEEGTVINRQNGEKVMADDEFTQDLFRFLQLLCEGHNNGSYVRGERGGSTPINIIICSGLLLRAGEGPSGFTGTGGNGQDGRNFSKAMAVAKQVFNSLTEYIQVVTLSHPWPGPQSLLAFQDSSQIELLKELLDLQKDIGVWLCCGRSPPPKPPRSPQEKSQDQRNPHPGWSLTDTAMVLLTADAPPLTPRPPAGNVVNGTIARQMVDMLVESSSNVEMILKFFDMFLKLKDIVGSEAFQDYVTDPRGLISKKDFQKAMDSQKQFTGPEIQFLLSCSEADENEMINVEEFASRFQEPARDIGFNVAVLLTNLSEHVPHDPRLKNFLELAESILEYFRPYLGRIEIMGASRRIERIYFEISETNKAQWEMPQVKESKRQFIFDVVNEGGESEKMEMFVSFCEDTIFEMQIAAQISEPEGEPEEDEEESMAESGPDSGEAGPDGEGAGAGGPTGISGHVAGAVSSVLRKLTYRSLRRRVRRLRKLTLREAAGTGAGLIWAGATWTGSGVIGATRGALGLLWGSLFGGGLVEGAKKVTVTGLLAGMPDPTGDEVHGEQAAGAGAAGTGDGAVEGDEEVGAELAEREAEAEAGAGEAGPAGGEGAGANGGHFRMEVPGGLGDMGDTTPVEPPTPEGSPIFRRKLGLEGEEEQALEPEPEPEPEPEPEPEPEPEKADAENGEKEGSQEPEPEAPKKAAAPPPPRKEDAKSEGPDFWEEMEVQRVKFLNYLSRNFYTLRFLALFLAFAINFILLFYKVSDSPPGEEDLEGSGLEGLAGSGSGDFLGSGMGSGAGEDLENEEEENMVYYFLEESTGYMEPALRGLSILHTLIAFLCIIGYNCLKVPLVIFKREKELARKLEFDGLYITEQPEDDDVKGQWDRLVLNTPSFPSNYWDKFVKRKVLEKHGDIFGRERIAELLGMDLASLEITAQNEKRPEPPSGLLTWIMSIDVKYQIWKFGVIFTDNSFLYLTWYMVMSLLGHYNNFFFAAHLLDIAMGVKTLRTILSSVTHNGKQLVMTVGLLAVVVYLYTVVAFNFFRKFYNKSEDEDEPDMKCDDMMTCYLFHMYVGVRAGGGIGDEIEDPAGDEYELYRVVFDITFFFFVIVILLAIIQGLIIDAFGELRDQQEQVKEDMETKCFICGIGSDYFDTTPHGFETHTLEEHNLANYMFFLMYLINKDETEHTGQESYVWKMYQERCWDFFPAGDCFRKQYEDQLA

Target ORF information:

RefSeq Version	XM_031961617.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii ryanodine receptor 1 (RYR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031961617.1

ORF Insert Sequence:

ATGGCGGACG TCCTGGATAG GATGGTACTG CGGTGGCTTC CGCTCGTACG GGGTACCAAT 
GCGGGCGGGT TAGCCCTGAA TGACGGCCGG TGGTGCGGTA TGCGGTCCGT GGACCGGGCG 
GTGGCAGCGC GTCATCGGGT TGGCGCGCGG CGGGCCATCG GTTCTCTGAC CTGTCGTCGG 
TCCTGGATGG CGACGCTCGC GCGCTCCCAT GCTCAGCAGG TACGCCATTG CTGGCGCCCG 
AGACCAGCAC CATCACCCTG TTCCCGCGGA GCTGTCGCCC GGCGAGGCTT CATGGCGCGG 
GCTGCCGAGT GGGCGCCTCC CGTCCTCACG CCCGCCCTAC ACTTCGCCGC CCTGCCCATT 
GGCAGCGCCC AGCGCCCGGG AGGCCAGCAC GCCCGCGACC CCGTGGGGGG CTCTGTGGAG 
TTCCAGTTCG TTCCGGTGCT CAAACTGGTG TCCACCCTGC TGGTAACCAC CGCGTCCCTC 
CACCCACCCT TCCTCCCCCC ACTCTGCCCT CAGGCTTCAG TCTCCAACTC TGTCCGCCAA 
TCGTTCCCTT CATCCGGCAG TCACTCGGTC ATCCGTCAGT CATTGGTTCA TCCGTCAGTC 
ATTGGTTCAT CCGTCAGTTG CCCACCCCTG AACTTGGGCA CTTCCCCCGT CCGTCAGTCC 
CCCCTCCCCC ATCCGTCAGT CCCCTACGAA ATCCGCTTCC CCAAAATGGT CACCAGCTGC 
TGCCGGTTCC TCTGCTATTT CTGTCGCATT AGTCGCCAGA ACCAAAGGTC CATGTTTGAT 
CACCTCAGCT ACCTGCTGGA GAACAGCGGG ATCGGCCTGG GTATGCAGGG TTCCACTCCG 
CTAGATGTGG CGGCTGCCTC CGTCATAGAC AACAACGAGC TGGCCTTGGC CCTTCAGGAA 
CAAGACCTGG AAAAGGTCGT CTCCTACTTG GCCGGCTGCG GCCTTCAGAG CTGCCCCATG 
CTGCTGGCCA AGGGCTACCC CGACATTGGC TGGAACCCCT GCGGGGGGGA GCGCTATCTG 
GATTTCCTGA GATTTGCCGT CTTTGTTAAT GGAGAGAGCG TGGAAGAGAA TGCGAATGTT 
GTGGTTCGTC TGCTCATCCG GCGTCCCGAA TGCTTCGGCC CGGCCCTGCG AGGAGAGGGC 
GGCTCTGGGC TGCTGGCTGC TATGGAAGAG GCAATCCGGA TCTCAGAGGA TCCTGCTCGA 
GATGGCCCGG GCGTCAGGAA GGACCGGAGG CGGGAGCACC ACGGGGGTGA AGAGGCCCCT 
GAAGAAAACC GTGTGCACTT GGGAAATGCC ATCATGTCAT TCTATTCCGC CCTGATCGAC 
CTGCTGGGAC GCTGTGCTCC AGAGACTCAT CTGATCCAGG CCGGGAAGGG AGAAGCGCTG 
AGGATCCGAG CCATTCTGCG CTCCCTGGTG CCCCTGGACG ACCTCGTGGG CATTATCAGC 
CTGCCCCTGC AGATCCCCAC CCTGGGCAAA GATGGGGCCC TGGTGCAGCC TCGGATGTCT 
GCCTCCTTCG TGCCTGACCA CAAAGCCCCC ATGGTCCTCT TCCTGGACCG CGTCTACGGC 
ATCGACAGCC AGGACTTCCT GCTCCACGTG CTTGATGCGG GTTCCCCTGG CGGGCGGTGC 
CTCCCTGGAC CGGGGGGCGC AACCTTTCCA AAGGAGATGG CCCTGGCCTT GAACCCTACT 
TGCACACCTG TTTATCCCCA AGTCCCCCCA CTGTTTGGGN GAAGGGCCGC CATCATGGTG 
GACTCCATGC TCCACACGGT GTACCGCCTG TCCCGGGGAC GCTCTCTCAC CAAGGCCCAG 
CGAGATGTCA TTGAGGACTG CCTCATGGCG CTCTGCAGGT ACATCCGTCC ATCTATGCTC 
CAGCACCTTC TGCGTCGTCT GGTATTCGAC GTTCCCATCC TCAACGAGTT TGCCAAGATG 
CCCCTCAAGC TCCTGACCAA TCACTATGAG CGCTGCTGGA AATATTACTG CCTCCCTACG 
GGCTGGGCCA ACTATGGAGT TACCTCCGAG GAAGAGCTGC ATCTGACTCG CAAGCTTTTC 
TGGGGCATCT TTGAGTCACT GGCCCACAAG AAATACGACC AGGAGCTCTA CCGCATGGCC 
ATGCCGTGTC TGTGCGCCAT CGCCGGGGCC CTGCCCCCCG ACTACGTGGA TGCCTCCTAC 
TCCTCCAAAG CTGAGAAGAA GGCCACTGTG GATGCTGAGG GCAACTTTGA CCCCCGGCCC 
GTGGAGACAC TCAATGTGAT CATACCCGAG AAACTCGACT CCTTCATCAA CAAATTTGCC 
GAGTACACAC ACGAGAAGTG GGCATTTGAC AAGATTCAGA ACAATTGGTC ATTTGGGGAG 
AACATTGACG AGGAGCTCAA GACCCACCAC ATGCTTCGGC CCTACAAGAC CTTCTCAGAA 
AAGGACAAGG AGATTTACCG ATGGCCCATC AAGGAGTCCC TGAAGGCCAT GATCGCCTGG 
GAGTGGACTG TGGAAAAGGC CCGTGAGGGT GAGGAGGAGA AGGGAGAGAA GAAGACCAAG 
ACTCGCAAGA TCTCCCAGAA TACCCCGGCC ACCTATGACC CAAGAGAGGG CTATAATCCC 
CAGCCTCCAG ACCTTACCTG TGTCACCCTG TCCCGGGAAC TGCAGGCCAT GGCTGAACAA 
CTGGCTGAGA ATTACCATAA CACATGGGGG AGGAAGAAAA AGCAGGAATT AGAAGCCAAG 
GGTGGAGGCA CCCACCCCCT CCTGGTCCCC TATGACACAC TGACGGCCAA GGAGAAGGCT 
CGCGATAGAG AGAAAGCCCA GGAACTGCTC AAATTCCTGC AGATGAACGG CTACGCGGTG 
ACGCGAGGGC TGAAGGACAT GGAGCTGGAC ACATCTTCAA TTGAGAAGAG GTTTGCGTTT 
GGGTTCCTCC AGCAGCTGCT CCGATGGATG GACATCTCAC AGGAGTTCAT TGCCCATCTG 
GAGGCTGTGG TGAGCAGCGG ACGGGTGGAG AAGTCTCCTC ACGAGCAAGA GATCAAGTTC 
TTTGCCAAGA TCCTCCTGCC GCTCATCAAC CAGTACTTCA ACAACCACTG CCTCTACTTT 
CTGTCCACAC CAGCCAAGGT GCTCGGCAGT GGGGGCCACG CCTCTAACAA GGAAAAGGAG 
ATGATCACCA GCCTGTTCTG TAAACTCGCC GCTCTGGTCC GCCACCGAGT CTCTCTCTTT 
GGGACTGACG CTCCAGCTGT GGTCAACTGC CTGCACATCC TCGCCCGGTC CCTGGATGCA 
AGGACCCACC AGTTTGGGGA TGATGTCATC TTGGACGACG TCCAGGTCTC CTGCTACCGA 
ACACTTTGCA GCATCTACTC TCTGGGGACC ACCAAGAATT CCTATGTGGA GAAGCTCCGT 
CCAGCCCTTG GAGAATGCCT TGCCCGTCTG GCAGCTGCTA TGCCAGTTGC CTTCCTTGAA 
CCCCAACTGA ATGAGTTCAA CGCCTGCTCT GTCTACACCA CCAAATCTCC CCGAGAACGT 
GCCATCCTGG GCCTTCCGAG CTCCGTGGAG GAGATGTGCC CAGATATCCC AGACCTGAAG 
CACCTGATGG CCGAGATCGG GGGGCTGGCC GAGTCGGGCG CCCGTCACAC CGAGATGCCC 
CATGTCATCG AGATCACGCT GCCGATGCTC TGTAACTACC TGCCCCGCTG GTGGGAGCGG 
GGGCCCGAGG TCCCCTCCTC ACAGCCCCCA GGGGGACCCC CACCCTGCAC ANNGATGCAC 
CCGGACCACC CCTAATCCCT CCTAAACATC CTCCGATTTG TCAACAACCT GGGATAAGGG 
GTTTTCGTGG ATGGCGCTCA GGGGGACCGG AATGAAGCGC TTGGGGTGTG GATGTGGAGG 
CTCCCCAGGC CCACCAACGC CCCTGCCTCG TGTCCCGCAG TGTTTGCGCA GCCGATCGTG 
AGCCGGGCCC GGCCCGAGCT GCTCCGCTCA CATTTCATCC CCACGGTGGG CCGGCTCCGG 
AAGCGCGCAG GGAAGGTGGT GGCCGAGGAA GAGCAGCTGA GGCTGGAAGC CAAGGCGGAG 
GCTGAGGAGG GAGAGCTCCT GGTGCGGGAC GAGTTCTCTG TGCTCTGCCG GGATCTCTAC 
GCACTCTACC CCCTGCTCAT CCGCTACGTA GACAACAACC GAGCCCACTG GCTAACAGAA 
CCCAACCCTG ATGCAGAAGA ACTCTTCAGG ATGATAGCAG AAATCTTCAT CTATTGGTCC 
AAGTCTCATA ACTTCAAGAG AGAAGAACAG AATTTCGTGG TACAGAACGA AATCAATAAC 
ATGTCCTTCC TGACGGCAGA TAGCAAGAGC AAGATGGCCA AGGCGGGAGA TGGACAGTCA 
GGAGGCTCCG ATCAAGAGAG AACAAAGAAG AAACGGCGAG GGGACCGATA TTCCGTCCAG 
ACCTCTCTGA TCGTGGCTAC CCTCAAGAAG ATGCTGCCTA TTGGGTTGAA CATGTGTGCA 
CCCACTGACC AGGACCTCAT CGCGCTGGCC AAGACTCGTT ATGCCCTGAA AGACACAGAT 
GAAGAAGTTC GGGAATTTCT GCAGAATAAC CTTCATCTTC AGGGAAAGAG TGAGGGCTCC 
CCATCACTGC GCTGGCAGAT GGCCCTGTAC CGGGGGCTGC CTGGCCGGGC TGAGGATGCT 
GATGCCCCTG AGAACATTGT TCGCCGAGTA CAGGAAGTCT CAGCGGTGCT TTACCACCTG 
GAACAGACAG AACATCCCTA CAAGTCCAAG AAAGCTGTGT GGCACAAGCT GCTTTCAAAA 
CAGCGTCGTC GGGCGGTTGT GGCTTGTTTT CGCATGACAC CCTTGTACAA CTTGCCTCGG 
CATCGGGCAA GCAATATGTT CTTGGAGTCC TACAAAGCAT CATGGATCCT AACTGAGGAG 
CACAGTTTTG AGGACCGCAT GATTGATGAC CTTTCTAAAG CTGGGGAGGA AGAAGAAGAG 
GAAGAAGAAC AGGAAGAGAA GAAGCCAGAC CCTCTTCACC AGCTGGTATT ACACTTTAGC 
CGGACAGCCC TGACAGAGAA GAGCAAACTA GATGAGGACT ATTTGTACAT GGCATATGCT 
GACATCATGG CCAAGAGCTG CCACTTGGAG GAGGAAGAGG AGCCTTCAGA ACCTGCTGAG 
GAGGAGGCTG AAGTTTCATT TGAGGAGAAG GAGATGGAAA AGCAGAAACT GCTCTACCAG 
CAGTCAAGGC TACACAACCG AGGGGCTGCT GAGATGGTGC TACAAATGAT TAGTGCCTGC 
AAAGGGGAGC CGGGAGTCAT GGTTTCATCC ACCCTGAAGT TGGGCATCTC CATCCTCAAC 
GGTGGCAACA TGGAGGTGCA GCAGAAGATG CTGGACTATC TGAAGGACAA AAAGGATGTT 
GGCTTCTTCC AGAGCATCCA GGCCCTGATG CAGACATGCA GTGTTCTTGA TCTGAATGCC 
TTTGAGAGGC AGAACAAGGC AGAGGGGCTT GGAATGGTGA CCGAGGAAGG GACTGTCATC 
AATCGCCAGA ACGGGGAAAA AGTAATGGCC GACGATGAGT TCACCCAGGA TCTCTTCCGC 
TTTCTGCAGC TGCTGTGTGA AGGCCATAAC AATGGTTCGT ATGTGAGAGG AGAAAGAGGA 
GGGAGCACAC CCATCAACAT CATCATCTGC AGTGGATTAC TCTTGAGGGC TGGGGAGGGT 
CCATCAGGCT TTACCGGAAC TGGGGGAAAT GGACAAGACG GGAGGAACTT CTCCAAAGCC 
ATGGCCGTGG CCAAGCAGGT CTTCAACAGC CTCACCGAGT ACATCCAGGT AGTCACCTTG 
TCCCATCCGT GGCCCGGCCC CCAGTCCCTC CTGGCCTTCC AGGACTCAAG CCAGATTGAG 
CTGCTGAAGG AGCTCCTGGA TCTGCAGAAA GACATTGGGG TTTGGCTTTG CTGTGGAAGG 
TCCCCGCCAC CCAAACCACC CAGATCTCCC CAGGAGAAAT CCCAGGACCA GAGGAACCCT 
CATCCCGGCT GGAGCCTGAC AGACACGGCT ATGGTGCTCC TCACCGCTGA CGCTCCTCCC 
CTTACTCCCC GCCCCCCGGC AGGTAACGTG GTCAACGGCA CAATTGCCCG GCAGATGGTG 
GACATGCTGG TGGAGTCCTC CTCCAACGTG GAGATGATCC TCAAGTTCTT CGACATGTTC 
CTAAAGCTGA AGGACATCGT GGGCTCCGAG GCATTCCAGG ACTACGTCAC CGACCCCCGT 
GGACTCATCT CCAAGAAGGA TTTCCAGAAG GCCATGGACA GCCAGAAGCA GTTCACTGGC 
CCAGAGATCC AGTTCCTCCT GTCCTGCTCA GAGGCGGATG AGAACGAGAT GATCAATGTG 
GAGGAATTTG CCAGCCGCTT CCAGGAGCCG GCCCGAGACA TCGGCTTCAA CGTGGCCGTG 
CTGCTGACCA ACCTGTCGGA GCACGTGCCC CACGACCCGC GCCTCAAGAA CTTCCTGGAG 
CTGGCTGAGA GCATCCTGGA GTACTTCCGG CCCTACCTGG GCCGGATCGA GATCATGGGG 
GCCTCCCGGC GCATCGAGCG CATCTACTTT GAAATCTCTG AGACCAACAA AGCACAGTGG 
GAGATGCCCC AGGTGAAGGA GTCCAAACGC CAGTTCATCT TTGACGTGGT GAATGAAGGG 
GGGGAGTCGG AGAAGATGGA AATGTTTGTG AGCTTCTGTG AAGACACCAT CTTTGAGATG 
CAGATCGCCG CCCAGATCTC AGAGCCCGAG GGAGAGCCTG AGGAAGATGA GGAGGAGTCC 
ATGGCAGAGT CGGGGCCAGA CTCCGGGGAG GCAGGGCCCG ACGGAGAAGG GGCCGGCGCA 
GGGGGCCCGA CGGGCATCTC CGGGCACGTG GCCGGTGCCG TCTCCTCGGT CCTGCGGAAG 
CTGACCTACC GGAGCCTGCG CAGGAGGGTG CGGAGGCTCC GCAAGCTGAC CCTGCGGGAG 
GCTGCAGGAA CCGGGGCCGG GCTCATCTGG GCCGGGGCCA CGTGGACGGG CTCCGGAGTG 
ATCGGCGCCA CCCGGGGGGC CCTGGGCCTA CTCTGGGGCT CTCTGTTTGG GGGAGGGCTG 
GTGGAGGGAG CCAAGAAAGT GACCGTGACG GGACTGTTGG CCGGGATGCC AGACCCCACA 
GGCGATGAGG TCCATGGGGA GCAGGCGGCT GGCGCCGGGG CTGCCGGGAC TGGGGACGGA 
GCCGTGGAGG GTGATGAGGA GGTGGGGGCG GAGTTGGCCG AGAGGGAGGC TGAGGCTGAG 
GCCGGAGCTG GGGAGGCCGG GCCAGCCGGA GGAGAGGGAG CCGGGGCCAA TGGAGGCCAC 
TTCCGGATGG AGGTCCCCGG AGGCCTGGGT GACATGGGAG ACACAACACC CGTTGAGCCG 
CCCACTCCTG AGGGTTCCCC CATCTTCCGG AGGAAACTAG GGCTGGAGGG AGAAGAAGAA 
CAAGCCTTAG AACCAGAACC AGAGCCAGAA CCAGAACCAG AGCCAGAGCC AGAACCCGAG 
CCTGAGAAAG CTGATGCTGA GAACGGAGAA AAGGAAGGGT CCCAAGAGCC AGAACCTGAG 
GCTCCCAAGA AGGCAGCTGC CCCACCTCCG CCCCGGAAGG AGGACGCCAA ATCTGAGGGC 
CCTGACTTCT GGGAGGAAAT GGAGGTGCAG AGGGTCAAGT TCCTGAACTA CCTCTCCAGA 
AACTTCTATA CCCTGAGATT CCTCGCCCTC TTCCTGGCCT TTGCCATCAA CTTCATCCTG 
CTCTTCTACA AGGTGTCTGA CTCTCCGCCA GGAGAAGAGG ACCTGGAAGG CTCCGGTCTC 
GAGGGTCTGG CAGGCTCAGG GTCCGGGGAC TTCCTGGGAT CCGGCATGGG CTCCGGGGCT 
GGGGAGGATC TGGAGAACGA GGAGGAAGAG AACATGGTCT ACTACTTCCT GGAAGAGAGT 
ACGGGCTACA TGGAGCCAGC CCTGCGAGGT CTCAGCATCC TGCACACCCT CATTGCCTTC 
CTCTGCATCA TAGGCTACAA CTGTCTCAAG GTACCGCTGG TTATATTTAA ACGGGAGAAG 
GAGCTAGCCC GGAAACTAGA GTTCGATGGC CTTTACATCA CTGAGCAGCC CGAGGATGAC 
GACGTGAAGG GCCAGTGGGA TCGACTGGTG CTTAACACCC CGTCCTTCCC CAGCAACTAC 
TGGGACAAGT TCGTGAAAAG GAAGGTGCTG GAGAAACATG GCGACATCTT TGGCCGGGAG 
CGGATTGCAG AGCTGTTGGG CATGGATCTG GCCAGCCTGG AGATCACGGC GCAGAACGAG 
AAGCGACCGG AACCCCCCTC GGGACTGCTC ACCTGGATCA TGTCCATAGA TGTCAAATAC 
CAAATCTGGA AATTTGGTGT CATCTTTACA GATAATTCAT TCTTGTATTT GACGTGGTAC 
ATGGTCATGT CCCTCCTCGG CCACTATAAC AACTTCTTCT TTGCCGCTCA CCTGCTGGAC 
ATCGCCATGG GAGTCAAGAC GCTTCGGACC ATCCTGTCTT CTGTCACCCA CAATGGGAAG 
CAGCTGGTGA TGACGGTGGG CCTGCTGGCC GTCGTGGTTT ACCTCTACAC GGTTGTGGCC 
TTCAATTTCT TCCGCAAATT TTACAATAAA AGCGAGGATG AGGATGAGCC GGACATGAAA 
TGTGATGACA TGATGACGTG TTACCTGTTC CACATGTACG TGGGGGTCCG AGCTGGTGGG 
GGCATCGGGG ATGAGATTGA GGATCCAGCA GGGGATGAAT ACGAGCTCTA CCGTGTGGTC 
TTTGACATCA CCTTTTTCTT CTTCGTCATC GTCATCCTGC TGGCCATTAT CCAAGGTCTG 
ATCATTGATG CTTTTGGGGA GCTCCGAGAC CAGCAAGAGC AAGTGAAGGA AGATATGGAG 
ACCAAATGTT TCATCTGTGG CATTGGAAGT GACTACTTTG ATACAACACC CCACGGTTTT 
GAGACCCACA CGCTGGAGGA GCACAACCTG GCTAACTACA TGTTTTTCCT GATGTATCTG 
ATCAATAAGG ATGAGACCGA GCACACGGGA CAGGAGTCGT ATGTCTGGAA GATGTACCAG 
GAGCGCTGCT GGGACTTCTT CCCAGCTGGT GACTGTTTCC GAAAACAGTA TGAAGACCAG 
CTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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RYR1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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