GEN1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	GEN1
Entrez Gene ID	100916082
Full Name	GEN1, Holliday junction 5' flap endonuclease
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012540672.1	XP_012396126.1	flap endonuclease GEN homolog 1
XM_031949605.1	XP_031805465.1	flap endonuclease GEN homolog 1 isoform X2
XM_023495390.1	XP_023351158.1	flap endonuclease GEN homolog 1
XM_031949603.1	XP_031805463.1	flap endonuclease GEN homolog 1 isoform X1
XM_031949604.1	XP_031805464.1	flap endonuclease GEN homolog 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following GEN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GEN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa120590	XM_012540672.1 Latest version!	Sarcophilus harrisii GEN1 Holliday junction 5' flap endonuclease (GEN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSa151189	XM_023495390.1 Latest version!	Sarcophilus harrisii GEN1, Holliday junction 5' flap endonuclease (GEN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSa223467	XM_031949603.1 Latest version!	Sarcophilus harrisii GEN1 Holliday junction 5' flap endonuclease (GEN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSa223467	XM_031949604.1 Latest version!	Sarcophilus harrisii GEN1 Holliday junction 5' flap endonuclease (GEN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSa223468	XM_031949605.1 Latest version!	Sarcophilus harrisii GEN1 Holliday junction 5' flap endonuclease (GEN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GEN1 ( XM_012540672.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012396126.1 Sarcophilus harrisii GEN1 Holliday junction 5' flap endonuclease (GEN1), mRNA.

Price & Availability↑

CloneID	OSa120590
Clone ID Related Accession (Same CDS sequence)	XM_012540672.1
Accession Version	XM_012540672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	flap endonuclease GEN homolog 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816913.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 48% of CDS bases ##RefSeq-Attributes-END##

ATGGGGGTGA ATGATCTGTG GCAGATTCTG GAGCCTGTTA AGCGGCACGC CCCTCTCCGG 
AGTCTCCAGG GGAAGACCCT GGCCGTTGAC CTGAGTCTCT GGGTATGCGA AGCACTTGCT 
GTCAAAAAGA TGGTCGGCAT CGTTGTCAAG CCCCACCTCA GGAACCTGTT TTTTCGCATC 
TCATCTCTAA CCTTAATGGA AGTAAAACTG CTCTTCGTCA TGGAAGGCGA CGCTCCCAAG 
CTGAAAGCAG ACGTGATGGG CAAACGGAAC GAGACGCGCT TCGGGGCCCC CCAAAACCGG 
GCCCACAAAT TCACAAGATC CTCTTTCAAG GCTGTCTTAA AAGAGTGCCT TGACTTGTTG 
GAGTGCTTGG GAGTTCCGTG GGTTCAGGCA TCCGGGGAAG CCGAGGCTAT GTGCGCCTAC 
CTCGATGCCC ATGGCTATGT GGACGGGTGC CTCACTGACG ATGGAGACGC TTTCCTTTAC 
GGAGCACAGA CTGTTTATCG AAATTTCACT ATGAATGCAA AGGACCCCCA TGTGGATTGC 
TACACGATGT CCTCGATTAA GCGGGATCTG GGCCTGGATC GGGACACCCT GATTGGCCTG 
GCCGTGCTGC TGGGCTGTGA CTACCTTCCG AAGGGAGTCC CTGGAGTTGG AAAGGAGCTG 
GCGCTAAAGC TTGTGCGCTC TTTGAAAGGG CAGAACTTGC TGCAGAAGTT CGATCAGTGG 
AAGGAGGAGT CCCACAGCCC CGACCTGCCG CCAGCCCCAG TTAGGAAGCC AGCTCATTGC 
AATGTGTGCG CCCATCCAGG GCTCCCCAGC ATCGGTGACT TTCCCAGGGA ATCCCATCTT 
CTCACGGCCA GAGTTCCGGC ACTTGCTCGG GGGCAGCCTG GTGATGTCAG TGGGCACTGG 
CGCAGTCCCT TGCTGTCCGA GGGCCAGCCC CCGTCGACGG CCATCCTCGA AACCCAAGAA 
CGTGGCATCC GTCCCTGCTT CCAGTGGCTC TTCCAGGAGC TCACGGGGGC TCCTTCCACA 
AAGTCTGCAG GCCCAGCCCA GGCGGAGCGT CAGCGGGAAG GGAGCTCGCA GCGAGGCCGT 
GGGAGCAAAG ATGCGACACC CACTCCACGA GTGCCCCAGA GACCCGGAGG GGACCCCGGG 
GGGCGGATGC TGGCCCAGGG AAGGAAAGCT TGCGCTTGCC AAGGATTCCC GTTCCCTGAG 
GTCATTCGAG AATTCCAGGT AAACAAGGAT CGAGTGACCA AGAAAATCTG CTTCCGGAGA 
CCTGACCTCT TGTCCTTCCA GATTTTTGCC TTGGAGAAGC TGGAGTGGCC GCGGCACTAC 
GCCTGCGAGA AGCTCATGGG GCTCTTGGCC CATCACGACA TGGTGGAACG GAAGCTTGGG 
CACAAGGGCC CCGCCCAGCT GCGGCCCCTG CGAATAACCC GCTCCCGCCT CAGGAGCGGC 
GTCCCTTGCT TGGAGATCCT GTGGCAGAAG CCAGAGCACT ACGTAATGGC CAGCGCGGCT 
CCCCAGGCCG GGCCACTCGC CGTGCTCACG GTGGAGGAAG CCGCCCTGTT TGAAGCTGCC 
TACCCCGAGG TGGTCGCCCA GTACCAGGAG CAGCTGCAGG AAAACCAGGG GAAGAAGTCC 
AAGAGTAAGA AGAACAAGGA CAAGGGGGGC GATCTGCTGG ATGTGGGCGA GGTGACCGGC 
CTTCTGGCCC ACGTGAGACT CTCCCACGAG ATGGGCCCGG GACAGGCCGC CAAGGCAGCC 
CTGAAAGCGC CCCCAGAGGA GGAATGGCCG CAGAGAAACA GCCCCGTGGG GGGCAGCCGG 
CTTTGGAGTG GGGCTCCCCT AAGTCTGGAA GCCTGGCAGC TGCCCGCCCC CACCCCCACC 
CCCGCTTCCA CCCCCACCCC CCTGAGACTC GGGGGCTCAC AGCACGGTTG GGCCAGCAGC 
CATCTGCTGT CCATGTCCGA GCTCCAGGAC GATAGCTCGG CCATCGAGCC ACTGGCGCAG 
CCCCAGCTCT CGGCTCCGGA CGGGCGCCAC GGCCACTGGG CCGACCCGGA AGCCTCGGCG 
CTGCAGAGGA AAGGGGCCTT CACTGCGAAA AGCGGCCCCT CCTTCGAGCC CCAGCAGCCT 
AACTGTAGCC CCCCAGGTCT TTTCCCAGTG TTCCGTTTGC AGAGCAGCCT GGACCAGTCT 
GACATGGCAA GCAGGAGGGC ACCCAGAGCT GGAGCGTTTG CGCCCCTTGG CTCCGGCAAT 
CCAACGCTGA GACCCCTGGG CCGGGGGCTC CCCCGACTTC ATCCCGAGGC TCAGGCTACT 
CCGAAGAGAT TCCCCAAAAA GACCGTCTGC CTCCCCTGCA GCTCCTCAGA GGAGGAGGAC 
GAGCTGACTA GCAGGAGCGA GAAGTGCCTG CGGCCGAAGG GGAAGCCCGA GCAGCGCCCT 
CCGGCCTGGC TCCAGACCAG TGTCCCGCGG CCAGATCCCA GAACCCTCTG TAAGGGAGCC 
GGGGCTCCAT GCTCGGCCAC GGCCACCTCC ACATTCGACC TCCTGGACGC TGCGGGGAGC 
TCGCTGCGGC CAGGCCTGTG CCCGTCCCCG CCCGGGGAAA CGAAGGCTGC CAGTCTGCTC 
CTGGAAAGCC CTCTGCCTTT GGCCCAGAGA CTGAAACTGC GATTCCAGAG CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012396126.1
CDS	1..2637
Translation	MGVNDLWQILEPVKRHAPLRSLQGKTLAVDLSLWVCEALAVKKMVGIVVKPHLRNLFFRISSLTLMEVKLLFVMEGDAPKLKADVMGKRNETRFGAPQNRAHKFTRSSFKAVLKECLDLLECLGVPWVQASGEAEAMCAYLDAHGYVDGCLTDDGDAFLYGAQTVYRNFTMNAKDPHVDCYTMSSIKRDLGLDRDTLIGLAVLLGCDYLPKGVPGVGKELALKLVRSLKGQNLLQKFDQWKEESHSPDLPPAPVRKPAHCNVCAHPGLPSIGDFPRESHLLTARVPALARGQPGDVSGHWRSPLLSEGQPPSTAILETQERGIRPCFQWLFQELTGAPSTKSAGPAQAERQREGSSQRGRGSKDATPTPRVPQRPGGDPGGRMLAQGRKACACQGFPFPEVIREFQVNKDRVTKKICFRRPDLLSFQIFALEKLEWPRHYACEKLMGLLAHHDMVERKLGHKGPAQLRPLRITRSRLRSGVPCLEILWQKPEHYVMASAAPQAGPLAVLTVEEAALFEAAYPEVVAQYQEQLQENQGKKSKSKKNKDKGGDLLDVGEVTGLLAHVRLSHEMGPGQAAKAALKAPPEEEWPQRNSPVGGSRLWSGAPLSLEAWQLPAPTPTPASTPTPLRLGGSQHGWASSHLLSMSELQDDSSAIEPLAQPQLSAPDGRHGHWADPEASALQRKGAFTAKSGPSFEPQQPNCSPPGLFPVFRLQSSLDQSDMASRRAPRAGAFAPLGSGNPTLRPLGRGLPRLHPEAQATPKRFPKKTVCLPCSSSEEEDELTSRSEKCLRPKGKPEQRPPAWLQTSVPRPDPRTLCKGAGAPCSATATSTFDLLDAAGSSLRPGLCPSPPGETKAASLLLESPLPLAQRLKLRFQSS

Target ORF information:

RefSeq Version	XM_012540672.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii GEN1 Holliday junction 5' flap endonuclease (GEN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012540672.1

ORF Insert Sequence:

ATGGGGGTGA ATGATCTGTG GCAGATTCTG GAGCCTGTTA AGCGGCACGC CCCTCTCCGG 
AGTCTCCAGG GGAAGACCCT GGCCGTTGAC CTGAGTCTCT GGGTATGCGA AGCACTTGCT 
GTCAAAAAGA TGGTCGGCAT CGTTGTCAAG CCCCACCTCA GGAACCTGTT TTTTCGCATC 
TCATCTCTAA CCTTAATGGA AGTAAAACTG CTCTTCGTCA TGGAAGGCGA CGCTCCCAAG 
CTGAAAGCAG ACGTGATGGG CAAACGGAAC GAGACGCGCT TCGGGGCCCC CCAAAACCGG 
GCCCACAAAT TCACAAGATC CTCTTTCAAG GCTGTCTTAA AAGAGTGCCT TGACTTGTTG 
GAGTGCTTGG GAGTTCCGTG GGTTCAGGCA TCCGGGGAAG CCGAGGCTAT GTGCGCCTAC 
CTCGATGCCC ATGGCTATGT GGACGGGTGC CTCACTGACG ATGGAGACGC TTTCCTTTAC 
GGAGCACAGA CTGTTTATCG AAATTTCACT ATGAATGCAA AGGACCCCCA TGTGGATTGC 
TACACGATGT CCTCGATTAA GCGGGATCTG GGCCTGGATC GGGACACCCT GATTGGCCTG 
GCCGTGCTGC TGGGCTGTGA CTACCTTCCG AAGGGAGTCC CTGGAGTTGG AAAGGAGCTG 
GCGCTAAAGC TTGTGCGCTC TTTGAAAGGG CAGAACTTGC TGCAGAAGTT CGATCAGTGG 
AAGGAGGAGT CCCACAGCCC CGACCTGCCG CCAGCCCCAG TTAGGAAGCC AGCTCATTGC 
AATGTGTGCG CCCATCCAGG GCTCCCCAGC ATCGGTGACT TTCCCAGGGA ATCCCATCTT 
CTCACGGCCA GAGTTCCGGC ACTTGCTCGG GGGCAGCCTG GTGATGTCAG TGGGCACTGG 
CGCAGTCCCT TGCTGTCCGA GGGCCAGCCC CCGTCGACGG CCATCCTCGA AACCCAAGAA 
CGTGGCATCC GTCCCTGCTT CCAGTGGCTC TTCCAGGAGC TCACGGGGGC TCCTTCCACA 
AAGTCTGCAG GCCCAGCCCA GGCGGAGCGT CAGCGGGAAG GGAGCTCGCA GCGAGGCCGT 
GGGAGCAAAG ATGCGACACC CACTCCACGA GTGCCCCAGA GACCCGGAGG GGACCCCGGG 
GGGCGGATGC TGGCCCAGGG AAGGAAAGCT TGCGCTTGCC AAGGATTCCC GTTCCCTGAG 
GTCATTCGAG AATTCCAGGT AAACAAGGAT CGAGTGACCA AGAAAATCTG CTTCCGGAGA 
CCTGACCTCT TGTCCTTCCA GATTTTTGCC TTGGAGAAGC TGGAGTGGCC GCGGCACTAC 
GCCTGCGAGA AGCTCATGGG GCTCTTGGCC CATCACGACA TGGTGGAACG GAAGCTTGGG 
CACAAGGGCC CCGCCCAGCT GCGGCCCCTG CGAATAACCC GCTCCCGCCT CAGGAGCGGC 
GTCCCTTGCT TGGAGATCCT GTGGCAGAAG CCAGAGCACT ACGTAATGGC CAGCGCGGCT 
CCCCAGGCCG GGCCACTCGC CGTGCTCACG GTGGAGGAAG CCGCCCTGTT TGAAGCTGCC 
TACCCCGAGG TGGTCGCCCA GTACCAGGAG CAGCTGCAGG AAAACCAGGG GAAGAAGTCC 
AAGAGTAAGA AGAACAAGGA CAAGGGGGGC GATCTGCTGG ATGTGGGCGA GGTGACCGGC 
CTTCTGGCCC ACGTGAGACT CTCCCACGAG ATGGGCCCGG GACAGGCCGC CAAGGCAGCC 
CTGAAAGCGC CCCCAGAGGA GGAATGGCCG CAGAGAAACA GCCCCGTGGG GGGCAGCCGG 
CTTTGGAGTG GGGCTCCCCT AAGTCTGGAA GCCTGGCAGC TGCCCGCCCC CACCCCCACC 
CCCGCTTCCA CCCCCACCCC CCTGAGACTC GGGGGCTCAC AGCACGGTTG GGCCAGCAGC 
CATCTGCTGT CCATGTCCGA GCTCCAGGAC GATAGCTCGG CCATCGAGCC ACTGGCGCAG 
CCCCAGCTCT CGGCTCCGGA CGGGCGCCAC GGCCACTGGG CCGACCCGGA AGCCTCGGCG 
CTGCAGAGGA AAGGGGCCTT CACTGCGAAA AGCGGCCCCT CCTTCGAGCC CCAGCAGCCT 
AACTGTAGCC CCCCAGGTCT TTTCCCAGTG TTCCGTTTGC AGAGCAGCCT GGACCAGTCT 
GACATGGCAA GCAGGAGGGC ACCCAGAGCT GGAGCGTTTG CGCCCCTTGG CTCCGGCAAT 
CCAACGCTGA GACCCCTGGG CCGGGGGCTC CCCCGACTTC ATCCCGAGGC TCAGGCTACT 
CCGAAGAGAT TCCCCAAAAA GACCGTCTGC CTCCCCTGCA GCTCCTCAGA GGAGGAGGAC 
GAGCTGACTA GCAGGAGCGA GAAGTGCCTG CGGCCGAAGG GGAAGCCCGA GCAGCGCCCT 
CCGGCCTGGC TCCAGACCAG TGTCCCGCGG CCAGATCCCA GAACCCTCTG TAAGGGAGCC 
GGGGCTCCAT GCTCGGCCAC GGCCACCTCC ACATTCGACC TCCTGGACGC TGCGGGGAGC 
TCGCTGCGGC CAGGCCTGTG CCCGTCCCCG CCCGGGGAAA CGAAGGCTGC CAGTCTGCTC 
CTGGAAAGCC CTCTGCCTTT GGCCCAGAGA CTGAAACTGC GATTCCAGAG CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEN1 ( XM_023495390.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023351158.1 Sarcophilus harrisii GEN1, Holliday junction 5' flap endonuclease (GEN1), mRNA.

Price & Availability↑

CloneID	OSa151189
Clone ID Related Accession (Same CDS sequence)	XM_023495390.1
Accession Version	XM_023495390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	flap endonuclease GEN homolog 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816913.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 30% of CDS bases ##RefSeq-Attributes-END##

ATGGGGGTGA ATGATCTGTG GCAGATTCTG GAGCCTGTTA AGCGGCACGC CCCTCTCCGG 
AGTCTCCAGG GGAAGACCCT GGCCGTTGAC CTGAGTCTCT GGGTATGCGA AGCACTTGCT 
GTCAAAAAGA TGGTCGGCAT CGTTGTCAAG CCCCACCTCA GGAACCTGTT TTTTCGCATC 
TCATCTCTAA CCTTAATGGA AGTAAAACTG CTCTTCGTCA TGGAAGGCGA CGCTCCCAAG 
CTGAAAGCAG ACGTGATGGG CAAACGGAAC GAGACGCGCT TCGGGGCCCC CCAAAACCGG 
GCCCACAAAT TCACAAGATC CTCTTTCAAG GCTGTCTTAA AAGAGTGCCT TGACTTGTTG 
GAGTGCTTGG GAGTTCCGTG GGTTCAGGCA TCCGGGGAAG CCGAGGCTAT GTGCGCCTAC 
CTCGATGCCC ATGGCTATGT GGACGGGTGC CTCACTGACG ATGGAGACGC TTTCCTTTAC 
GGAGCACAGA CTGTTTATCG AAATTTCACT ATGAATGCAA AGGACCCCCA TGTGGATTGC 
TACACGATGT CCTCGATTAA GCGGGATCTG GGCCTGGATC GGGACACCCT GATTGGCCTG 
GCCGTGCTGC TGGGCTGTGA CTACCTTCCG AAGGGAGTCC CTGGAGTTGG AAAGGAGCTG 
GCGCTAAAGC TTGTGCGCTC TTTGAAAGGG CAGAACTTGC TGCAGAAGTT CGATCAGTGG 
AAGGAGGAGT CCCACAGCCC CGACCTGCCG CCAGCCCCAG TTAGGAAGCC AGCTCATTGC 
AATGTGTGCG CCCATCCAGG TAGGAGACAT CCGTGGGGTG TCCAGTCGTC CGGCCCCGAG 
GAACACTGCT CGCTGGCCCT TCTGCCCCTG CCGGCCTGCA GTCCGGCCGG GCTCACGCTT 
GTGGCTCCCT CTCCCCCTCC CCTTCTCCCT TCAGGGCTCC CCAGCATCGG TGACTTTCCC 
AGGGAATCCC ATCTTCTCAC GGCCAGAGTT CCGGCACTTG CTCGGGGGCA GCCTGGTGAT 
GTCAGTGGGC ACTGGCGCAG TCCCTTGCTG TCCGAGGGCC AGGTGGGGCT CCCCGGTCCC 
CCAGTGGGAG GCCTGAGCCG GCAGGGCTCA CTTAGTCCCC CTCTCACAGC CGCACACGGC 
CCCTACCCCC GTCGACGGCC ATCCTCGAAA CCCAAGAACG TGGCATCCGT CCCTGCTTCC 
AGTGGCTCTT CCAGCAAAGA TGCGACACCC ACTCCACGAG TGCCCCAGAG ACCCGGAGGG 
GACCCCGGGG GGCGGATGCT GGCCCAGGGA AGACCTCGTG ACCTGTGGCT TGTTTCTAGG 
AAAGCTTGCG CTTGCCAAGG ATTCCCGTTC CCTGAGGTCA TTCGAGAATT CCAGGTAAAC 
AAGGATCGAG TGACCAAGAA AATCTGCTTC CGGAGACCTG ACCTCTTGTC CTTCCAGATT 
TTTGCCTTGG AGAAGCTGGA GTGGCCGCGG CACTACGCCT GCGAGAAGCT CATGGGGCTC 
TTGGCCCATC ACGACATGGT GGAACGGAAG CTTGGGCACA AGGGCCCCGC CCAGCTGCGG 
CCCCTGCGAA TAACCCGCTC CCGCCTCAGG AGCGGCGTCC CTTGCTTGGA GATCCTGTGG 
CAGAAGCCAG AGCACTACGT AATGGCCAGC GCGGCTCCCC AGGCCGGGCC ACTCGCCGTG 
CTCACGGTGG AGGAAGCCGC CCTGTTTGAA GCTGCCTACC CCGAGGTGGT CGCCCAGTAC 
CAGGAGCAGC TGCAGGAAAA CCAGGGGAAG AAGTCCAAGA GTAAGAAGAA CAAGGACAAG 
GGGGGCGATC TGCTGGATGT GGGCGAGGTG ACCGGCCTTC TGGCCCACGT GAGACTCTCC 
CACGAGATGG GCCCGGGACA GGCCGCCAAG GCAGCCCTGA AAGCGCCCCC AGAGGAGGAA 
TGGCCGCAGA GAAACAGCCC CGTGGGGGGC AGCCGGCTTT GGAGTGGGGC TCCCCTAAGT 
CTGGAAGCCT GGCAGCTGCC CGCCCCCACC CCCACCCCCG CTTCCACCCC CACCCCCCTG 
AGACTCGGGG GCTCACAGCA CGGTTGGGCC AGCAGCCATC TGCTGTCCAT GTCCGAGCTC 
CAGGACGATA GCTCGGCCAT CGAGGCCCCC CCACTGGCGC AGCCCCAGCT CTCGGCTCCG 
GACGGGCGCC ACGGCCACTG GGCCGACCCG GAAGCCTCGG CGCTGCAGAG GAAAGGGGCC 
TTCACTGCGA AAAGCGGCCC CTCCTTCGAG CCCCAGCAGC CTAACTGTAG CCCCCCAGGT 
CTTTTCCCAG TGTTCCGTTT GCAGAGCAGC CTGGACCAGT CTGACATGGC AAGCAGGAGG 
GCACCCAGAG CTGGAGCGTT TGCGCCCCTT GGCTCCGGCA ATCCAACGCT GAGACCCCTG 
GGCCGGGGGC TCCCCCGACT TCATCCCGAG GCTCAGGCTA CTCCGAAGAG ATTCCCCAAA 
AAGACCGTCT GCCTCCCCTG CAGCTCCTCA GAGGAGGAGG ACGAGCTGAC TAGCAGGAGC 
GAGAAGTGCC TGCGGCCGAA GGGGAAGCCC GAGCAGCGCC CTCCGGCCTG GCTCCAGACC 
AGTGTCCCGC GGCCAGATCC CAGAACCCTC TGTAAGGGAG CCGGGGCTCC ATGCTCGGCC 
ACGGCCACCT CCACATTCGA CCTCCTGGAC GCTGCGGGGA GCTCGCTGCG GCCAGGCCTG 
TGCCCGTCCC CGCCCGGGGA AACGAAGGCT GCCAGTCTGC TCCTGGAAAG CCCTCTGCCT 
TTGGCCCAGA GACTGAAACT GCGATTCCAG AGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023351158.1
CDS	22..2820
Translation	MGVNDLWQILEPVKRHAPLRSLQGKTLAVDLSLWVCEALAVKKMVGIVVKPHLRNLFFRISSLTLMEVKLLFVMEGDAPKLKADVMGKRNETRFGAPQNRAHKFTRSSFKAVLKECLDLLECLGVPWVQASGEAEAMCAYLDAHGYVDGCLTDDGDAFLYGAQTVYRNFTMNAKDPHVDCYTMSSIKRDLGLDRDTLIGLAVLLGCDYLPKGVPGVGKELALKLVRSLKGQNLLQKFDQWKEESHSPDLPPAPVRKPAHCNVCAHPGRRHPWGVQSSGPEEHCSLALLPLPACSPAGLTLVAPSPPPLLPSGLPSIGDFPRESHLLTARVPALARGQPGDVSGHWRSPLLSEGQVGLPGPPVGGLSRQGSLSPPLTAAHGPYPRRRPSSKPKNVASVPASSGSSSKDATPTPRVPQRPGGDPGGRMLAQGRPRDLWLVSRKACACQGFPFPEVIREFQVNKDRVTKKICFRRPDLLSFQIFALEKLEWPRHYACEKLMGLLAHHDMVERKLGHKGPAQLRPLRITRSRLRSGVPCLEILWQKPEHYVMASAAPQAGPLAVLTVEEAALFEAAYPEVVAQYQEQLQENQGKKSKSKKNKDKGGDLLDVGEVTGLLAHVRLSHEMGPGQAAKAALKAPPEEEWPQRNSPVGGSRLWSGAPLSLEAWQLPAPTPTPASTPTPLRLGGSQHGWASSHLLSMSELQDDSSAIEAPPLAQPQLSAPDGRHGHWADPEASALQRKGAFTAKSGPSFEPQQPNCSPPGLFPVFRLQSSLDQSDMASRRAPRAGAFAPLGSGNPTLRPLGRGLPRLHPEAQATPKRFPKKTVCLPCSSSEEEDELTSRSEKCLRPKGKPEQRPPAWLQTSVPRPDPRTLCKGAGAPCSATATSTFDLLDAAGSSLRPGLCPSPPGETKAASLLLESPLPLAQRLKLRFQSS

Target ORF information:

RefSeq Version	XM_023495390.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii GEN1, Holliday junction 5' flap endonuclease (GEN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023495390.1

ORF Insert Sequence:

ATGGGGGTGA ATGATCTGTG GCAGATTCTG GAGCCTGTTA AGCGGCACGC CCCTCTCCGG 
AGTCTCCAGG GGAAGACCCT GGCCGTTGAC CTGAGTCTCT GGGTATGCGA AGCACTTGCT 
GTCAAAAAGA TGGTCGGCAT CGTTGTCAAG CCCCACCTCA GGAACCTGTT TTTTCGCATC 
TCATCTCTAA CCTTAATGGA AGTAAAACTG CTCTTCGTCA TGGAAGGCGA CGCTCCCAAG 
CTGAAAGCAG ACGTGATGGG CAAACGGAAC GAGACGCGCT TCGGGGCCCC CCAAAACCGG 
GCCCACAAAT TCACAAGATC CTCTTTCAAG GCTGTCTTAA AAGAGTGCCT TGACTTGTTG 
GAGTGCTTGG GAGTTCCGTG GGTTCAGGCA TCCGGGGAAG CCGAGGCTAT GTGCGCCTAC 
CTCGATGCCC ATGGCTATGT GGACGGGTGC CTCACTGACG ATGGAGACGC TTTCCTTTAC 
GGAGCACAGA CTGTTTATCG AAATTTCACT ATGAATGCAA AGGACCCCCA TGTGGATTGC 
TACACGATGT CCTCGATTAA GCGGGATCTG GGCCTGGATC GGGACACCCT GATTGGCCTG 
GCCGTGCTGC TGGGCTGTGA CTACCTTCCG AAGGGAGTCC CTGGAGTTGG AAAGGAGCTG 
GCGCTAAAGC TTGTGCGCTC TTTGAAAGGG CAGAACTTGC TGCAGAAGTT CGATCAGTGG 
AAGGAGGAGT CCCACAGCCC CGACCTGCCG CCAGCCCCAG TTAGGAAGCC AGCTCATTGC 
AATGTGTGCG CCCATCCAGG TAGGAGACAT CCGTGGGGTG TCCAGTCGTC CGGCCCCGAG 
GAACACTGCT CGCTGGCCCT TCTGCCCCTG CCGGCCTGCA GTCCGGCCGG GCTCACGCTT 
GTGGCTCCCT CTCCCCCTCC CCTTCTCCCT TCAGGGCTCC CCAGCATCGG TGACTTTCCC 
AGGGAATCCC ATCTTCTCAC GGCCAGAGTT CCGGCACTTG CTCGGGGGCA GCCTGGTGAT 
GTCAGTGGGC ACTGGCGCAG TCCCTTGCTG TCCGAGGGCC AGGTGGGGCT CCCCGGTCCC 
CCAGTGGGAG GCCTGAGCCG GCAGGGCTCA CTTAGTCCCC CTCTCACAGC CGCACACGGC 
CCCTACCCCC GTCGACGGCC ATCCTCGAAA CCCAAGAACG TGGCATCCGT CCCTGCTTCC 
AGTGGCTCTT CCAGCAAAGA TGCGACACCC ACTCCACGAG TGCCCCAGAG ACCCGGAGGG 
GACCCCGGGG GGCGGATGCT GGCCCAGGGA AGACCTCGTG ACCTGTGGCT TGTTTCTAGG 
AAAGCTTGCG CTTGCCAAGG ATTCCCGTTC CCTGAGGTCA TTCGAGAATT CCAGGTAAAC 
AAGGATCGAG TGACCAAGAA AATCTGCTTC CGGAGACCTG ACCTCTTGTC CTTCCAGATT 
TTTGCCTTGG AGAAGCTGGA GTGGCCGCGG CACTACGCCT GCGAGAAGCT CATGGGGCTC 
TTGGCCCATC ACGACATGGT GGAACGGAAG CTTGGGCACA AGGGCCCCGC CCAGCTGCGG 
CCCCTGCGAA TAACCCGCTC CCGCCTCAGG AGCGGCGTCC CTTGCTTGGA GATCCTGTGG 
CAGAAGCCAG AGCACTACGT AATGGCCAGC GCGGCTCCCC AGGCCGGGCC ACTCGCCGTG 
CTCACGGTGG AGGAAGCCGC CCTGTTTGAA GCTGCCTACC CCGAGGTGGT CGCCCAGTAC 
CAGGAGCAGC TGCAGGAAAA CCAGGGGAAG AAGTCCAAGA GTAAGAAGAA CAAGGACAAG 
GGGGGCGATC TGCTGGATGT GGGCGAGGTG ACCGGCCTTC TGGCCCACGT GAGACTCTCC 
CACGAGATGG GCCCGGGACA GGCCGCCAAG GCAGCCCTGA AAGCGCCCCC AGAGGAGGAA 
TGGCCGCAGA GAAACAGCCC CGTGGGGGGC AGCCGGCTTT GGAGTGGGGC TCCCCTAAGT 
CTGGAAGCCT GGCAGCTGCC CGCCCCCACC CCCACCCCCG CTTCCACCCC CACCCCCCTG 
AGACTCGGGG GCTCACAGCA CGGTTGGGCC AGCAGCCATC TGCTGTCCAT GTCCGAGCTC 
CAGGACGATA GCTCGGCCAT CGAGGCCCCC CCACTGGCGC AGCCCCAGCT CTCGGCTCCG 
GACGGGCGCC ACGGCCACTG GGCCGACCCG GAAGCCTCGG CGCTGCAGAG GAAAGGGGCC 
TTCACTGCGA AAAGCGGCCC CTCCTTCGAG CCCCAGCAGC CTAACTGTAG CCCCCCAGGT 
CTTTTCCCAG TGTTCCGTTT GCAGAGCAGC CTGGACCAGT CTGACATGGC AAGCAGGAGG 
GCACCCAGAG CTGGAGCGTT TGCGCCCCTT GGCTCCGGCA ATCCAACGCT GAGACCCCTG 
GGCCGGGGGC TCCCCCGACT TCATCCCGAG GCTCAGGCTA CTCCGAAGAG ATTCCCCAAA 
AAGACCGTCT GCCTCCCCTG CAGCTCCTCA GAGGAGGAGG ACGAGCTGAC TAGCAGGAGC 
GAGAAGTGCC TGCGGCCGAA GGGGAAGCCC GAGCAGCGCC CTCCGGCCTG GCTCCAGACC 
AGTGTCCCGC GGCCAGATCC CAGAACCCTC TGTAAGGGAG CCGGGGCTCC ATGCTCGGCC 
ACGGCCACCT CCACATTCGA CCTCCTGGAC GCTGCGGGGA GCTCGCTGCG GCCAGGCCTG 
TGCCCGTCCC CGCCCGGGGA AACGAAGGCT GCCAGTCTGC TCCTGGAAAG CCCTCTGCCT 
TTGGCCCAGA GACTGAAACT GCGATTCCAG AGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEN1 ( XM_031949603.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031805463.1 Sarcophilus harrisii GEN1 Holliday junction 5' flap endonuclease (GEN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa223467
Clone ID Related Accession (Same CDS sequence)	XM_031949603.1 , XM_031949604.1
Accession Version	XM_031949603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	flap endonuclease GEN homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA ATGATCTGTG GCAGATTCTG GAGCCTGTTA AGCGGCACGC CCCTCTCCGG 
AGTCTCCAGG GGAAGACCCT GGCCGTTGAC CTGAGTCTCT GGGTATGCGA AGCACTTGCT 
GTCAAAAAGA TGGTCGGCAT CGTTGTCAAG CCCCACCTCA GGAACCTGTT TTTTCGCATC 
TCATCTCTAA CCTTAATGGA AGTAAAACTG CTCTTCGTCA TGGAAGGCGA CGCTCCCAAG 
CTGAAAGCAG ACGTGATGGG CAAACGGAAC GAGACGCGCT TCGGGGCCCC CCAAAACCGG 
GCCCACAAAT TCACAAGATC CTCTTTCAAG GCTGTCTTAA AAGAGTGCCT TGACTTGTTG 
GAGTGCTTGG GAGTTCCGTG GGTTCAGGCA TCCGGGGAAG CCGAGGCTAT GTGCGCCTAC 
CTCGATGCCC ATGGCTATGT GGACGGGTGC CTCACTGACG ATGGAGACGC TTTCCTTTAC 
GGAGCACAGA CTGTTTATCG AAATTTCACT ATGAATGCAA AGGACCCCCA TGTGGATTGC 
TACACGATGT CCTCGATTAA GCGGGATCTG GGCCTGGATC GGGACACCCT GATTGGCCTG 
GCCGTGCTGC TGGGCTGTGA CTACCTTCCG AAGGGAGTCC CTGGAGTTGG AAAGGAGCTG 
GCGCTAAAGC TTGTGCGCTC TTTGAAAGGG CAGAACTTGC TGCAGAAGTT CGATCAGTGG 
AAGGAGGAGT CCCACAGCCC CGACCTGCCG CCAGCCCCAG TTAGGAAGCC AGCTCATTGC 
AATGTGTGCG CCCATCCAGG CTCCCCCGGG GACCACGAGC TGCGCGGGTG CCGCCTGTGC 
GGCAGCGAGC GCTACTGCGA GCCCCACGAC CCCAAGTACC GCTGCCTGTG CGACTGGCAC 
CGCGCCGAGC ACCGCCGGCT ACAGGGTGCT GCCGAGAACA GCGTCAAGAA GAAAGCTTGC 
GCTTGCCAAG GATTCCCGTT CCCTGAGGTC ATTCGAGAAT TCCAGGTAAA CAAGGATCGA 
GTGACCAAGA AAATCTGCTT CCGGAGACCT GACCTCTTGT CCTTCCAGAT TTTTGCCTTG 
GAGAAGCTGG AGTGGCCGCG GCACTACGCC TGCGAGAAGC TCATGGGGCT CTTGGCCCAT 
CACGACATGG TGGAACGGAA GCTTGGGCAC AAGGGCCCCG CCCAGCTGCG GCCCCTGCGA 
ATAACCCGCT CCCGCCTCAG GAGCGGCGTC CCTTGCTTGG AGATCCTGTG GCAGAAGCCA 
GAGCACTACG TAATGGCCAG CGCGGCTCCC CAGGCCGGGC CACTCGCCGT GCTCACGGTG 
GAGGAAGCCG CCCTGTTTGA AGCTGCCTAC CCCGAGGTGG TCGCCCAGTA CCAGGAGCAG 
CTGCAGGAAA ACCAGGGGAA GAAGTCCAAG AGTAAGAAGA ACAAGGACAA GGGGGGCGAT 
CTGCTGGATG TGGGCGAGGT GACCGGCCTT CTGGCCCACG TGAGACTCTC CCACGAGATG 
GGCCCGGGAC AGGCCGCCAA GGCAGCCCTG AAAGCGCCCC CAGAGGAGGA ATGGCCGCAG 
AGAAACAGCC CCGTGGGGGG CAGCCGGCTT TGGAGTGGGG CTCCCCTAAG TCTGGAAGCC 
TGGCAGCTGC CCGCCCCCAC CCCCACCCCC GCTTCCACCC CCACCCCCCT GAGACTCGGG 
GGCTCACAGC ACGGTTGGGC CAGCAGCCAT CTGCTGTCCA TGTCCGAGCT CCAGGACGAT 
AGCTCGGCCA TCGAGGCCCT CCAGCTGAGC ACCATCGACT GGGAGGGCAC CTCTTTCAGC 
GTCTCCCCGG GGGCCCACTC CTCAGGGCCC AGCGGCGACA GCCCCCACGA GCCCCAGAGC 
ACCGGCTCGG CCAGAACCCA TTCGAGCCTC GGCTCGACCC TTCAGCCTCT GAAGGAGTGG 
GGGTGTGCCC AGCCACTGGC GCAGCCCCAG CTCTCGGCTC CGGACGGGCG CCACGGCCAC 
TGGGCCGACC CGGAAGCCTC GGCGCTGCAG AGGAAAGGGG CCTTCACTGC GAAAAGCGGC 
CCCTCCTTCG AGCCCCAGCA GCCTAACTGT AGCCCCCCAG GTCTTTTCCC AGTGTTCCGT 
TTGCAGAGCA GCCTGGACCA GTCTGACATG GCAAGCAGGA GGGCACCCAG AGCTGGAGCG 
TTTGCGCCCC TTGGCTCCGG CAATCCAACG CTGAGACCCC TGGGCCGGGG GCTCCCCCGA 
CTTCATCCCG AGGCTCAGGC TACTCCGAAG AGATTCCCCA AAAAGACCGT CTGCCTCCCC 
TGCAGCTCCT CAGAGGAGGA GGACGAGCTG ACTAGCAGGA GCGAGAAGTG CCTGCGGCCG 
AAGGGGAAGC CCGAGCAGCG CCCTCCGGCC TGGCTCCAGA CCAGTGTCCC GCGGCCAGAT 
CCCAGAACCC TCTGTAAGGG AGCCGGGGCT CCATGCTCGG CCACGGCCAC CTCCACATTC 
GACCTCCTGG ACGCTGCGGG GAGCTCGCTG CGGCCAGGCC TGTGCCCGTC CCCGCCCGGG 
GAAACGAAGG CTGCCAGTCT GCTCCTGGAA AGCCCTCTGC CTTTGGCCCA GAGACTGAAA 
CTGCGATTCC AGAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031805463.1
CDS	121..2721
Translation	MGVNDLWQILEPVKRHAPLRSLQGKTLAVDLSLWVCEALAVKKMVGIVVKPHLRNLFFRISSLTLMEVKLLFVMEGDAPKLKADVMGKRNETRFGAPQNRAHKFTRSSFKAVLKECLDLLECLGVPWVQASGEAEAMCAYLDAHGYVDGCLTDDGDAFLYGAQTVYRNFTMNAKDPHVDCYTMSSIKRDLGLDRDTLIGLAVLLGCDYLPKGVPGVGKELALKLVRSLKGQNLLQKFDQWKEESHSPDLPPAPVRKPAHCNVCAHPGSPGDHELRGCRLCGSERYCEPHDPKYRCLCDWHRAEHRRLQGAAENSVKKKACACQGFPFPEVIREFQVNKDRVTKKICFRRPDLLSFQIFALEKLEWPRHYACEKLMGLLAHHDMVERKLGHKGPAQLRPLRITRSRLRSGVPCLEILWQKPEHYVMASAAPQAGPLAVLTVEEAALFEAAYPEVVAQYQEQLQENQGKKSKSKKNKDKGGDLLDVGEVTGLLAHVRLSHEMGPGQAAKAALKAPPEEEWPQRNSPVGGSRLWSGAPLSLEAWQLPAPTPTPASTPTPLRLGGSQHGWASSHLLSMSELQDDSSAIEALQLSTIDWEGTSFSVSPGAHSSGPSGDSPHEPQSTGSARTHSSLGSTLQPLKEWGCAQPLAQPQLSAPDGRHGHWADPEASALQRKGAFTAKSGPSFEPQQPNCSPPGLFPVFRLQSSLDQSDMASRRAPRAGAFAPLGSGNPTLRPLGRGLPRLHPEAQATPKRFPKKTVCLPCSSSEEEDELTSRSEKCLRPKGKPEQRPPAWLQTSVPRPDPRTLCKGAGAPCSATATSTFDLLDAAGSSLRPGLCPSPPGETKAASLLLESPLPLAQRLKLRFQSS

Target ORF information:

RefSeq Version	XM_031949603.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii GEN1 Holliday junction 5' flap endonuclease (GEN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031949603.1

ORF Insert Sequence:

ATGGGGGTGA ATGATCTGTG GCAGATTCTG GAGCCTGTTA AGCGGCACGC CCCTCTCCGG 
AGTCTCCAGG GGAAGACCCT GGCCGTTGAC CTGAGTCTCT GGGTATGCGA AGCACTTGCT 
GTCAAAAAGA TGGTCGGCAT CGTTGTCAAG CCCCACCTCA GGAACCTGTT TTTTCGCATC 
TCATCTCTAA CCTTAATGGA AGTAAAACTG CTCTTCGTCA TGGAAGGCGA CGCTCCCAAG 
CTGAAAGCAG ACGTGATGGG CAAACGGAAC GAGACGCGCT TCGGGGCCCC CCAAAACCGG 
GCCCACAAAT TCACAAGATC CTCTTTCAAG GCTGTCTTAA AAGAGTGCCT TGACTTGTTG 
GAGTGCTTGG GAGTTCCGTG GGTTCAGGCA TCCGGGGAAG CCGAGGCTAT GTGCGCCTAC 
CTCGATGCCC ATGGCTATGT GGACGGGTGC CTCACTGACG ATGGAGACGC TTTCCTTTAC 
GGAGCACAGA CTGTTTATCG AAATTTCACT ATGAATGCAA AGGACCCCCA TGTGGATTGC 
TACACGATGT CCTCGATTAA GCGGGATCTG GGCCTGGATC GGGACACCCT GATTGGCCTG 
GCCGTGCTGC TGGGCTGTGA CTACCTTCCG AAGGGAGTCC CTGGAGTTGG AAAGGAGCTG 
GCGCTAAAGC TTGTGCGCTC TTTGAAAGGG CAGAACTTGC TGCAGAAGTT CGATCAGTGG 
AAGGAGGAGT CCCACAGCCC CGACCTGCCG CCAGCCCCAG TTAGGAAGCC AGCTCATTGC 
AATGTGTGCG CCCATCCAGG CTCCCCCGGG GACCACGAGC TGCGCGGGTG CCGCCTGTGC 
GGCAGCGAGC GCTACTGCGA GCCCCACGAC CCCAAGTACC GCTGCCTGTG CGACTGGCAC 
CGCGCCGAGC ACCGCCGGCT ACAGGGTGCT GCCGAGAACA GCGTCAAGAA GAAAGCTTGC 
GCTTGCCAAG GATTCCCGTT CCCTGAGGTC ATTCGAGAAT TCCAGGTAAA CAAGGATCGA 
GTGACCAAGA AAATCTGCTT CCGGAGACCT GACCTCTTGT CCTTCCAGAT TTTTGCCTTG 
GAGAAGCTGG AGTGGCCGCG GCACTACGCC TGCGAGAAGC TCATGGGGCT CTTGGCCCAT 
CACGACATGG TGGAACGGAA GCTTGGGCAC AAGGGCCCCG CCCAGCTGCG GCCCCTGCGA 
ATAACCCGCT CCCGCCTCAG GAGCGGCGTC CCTTGCTTGG AGATCCTGTG GCAGAAGCCA 
GAGCACTACG TAATGGCCAG CGCGGCTCCC CAGGCCGGGC CACTCGCCGT GCTCACGGTG 
GAGGAAGCCG CCCTGTTTGA AGCTGCCTAC CCCGAGGTGG TCGCCCAGTA CCAGGAGCAG 
CTGCAGGAAA ACCAGGGGAA GAAGTCCAAG AGTAAGAAGA ACAAGGACAA GGGGGGCGAT 
CTGCTGGATG TGGGCGAGGT GACCGGCCTT CTGGCCCACG TGAGACTCTC CCACGAGATG 
GGCCCGGGAC AGGCCGCCAA GGCAGCCCTG AAAGCGCCCC CAGAGGAGGA ATGGCCGCAG 
AGAAACAGCC CCGTGGGGGG CAGCCGGCTT TGGAGTGGGG CTCCCCTAAG TCTGGAAGCC 
TGGCAGCTGC CCGCCCCCAC CCCCACCCCC GCTTCCACCC CCACCCCCCT GAGACTCGGG 
GGCTCACAGC ACGGTTGGGC CAGCAGCCAT CTGCTGTCCA TGTCCGAGCT CCAGGACGAT 
AGCTCGGCCA TCGAGGCCCT CCAGCTGAGC ACCATCGACT GGGAGGGCAC CTCTTTCAGC 
GTCTCCCCGG GGGCCCACTC CTCAGGGCCC AGCGGCGACA GCCCCCACGA GCCCCAGAGC 
ACCGGCTCGG CCAGAACCCA TTCGAGCCTC GGCTCGACCC TTCAGCCTCT GAAGGAGTGG 
GGGTGTGCCC AGCCACTGGC GCAGCCCCAG CTCTCGGCTC CGGACGGGCG CCACGGCCAC 
TGGGCCGACC CGGAAGCCTC GGCGCTGCAG AGGAAAGGGG CCTTCACTGC GAAAAGCGGC 
CCCTCCTTCG AGCCCCAGCA GCCTAACTGT AGCCCCCCAG GTCTTTTCCC AGTGTTCCGT 
TTGCAGAGCA GCCTGGACCA GTCTGACATG GCAAGCAGGA GGGCACCCAG AGCTGGAGCG 
TTTGCGCCCC TTGGCTCCGG CAATCCAACG CTGAGACCCC TGGGCCGGGG GCTCCCCCGA 
CTTCATCCCG AGGCTCAGGC TACTCCGAAG AGATTCCCCA AAAAGACCGT CTGCCTCCCC 
TGCAGCTCCT CAGAGGAGGA GGACGAGCTG ACTAGCAGGA GCGAGAAGTG CCTGCGGCCG 
AAGGGGAAGC CCGAGCAGCG CCCTCCGGCC TGGCTCCAGA CCAGTGTCCC GCGGCCAGAT 
CCCAGAACCC TCTGTAAGGG AGCCGGGGCT CCATGCTCGG CCACGGCCAC CTCCACATTC 
GACCTCCTGG ACGCTGCGGG GAGCTCGCTG CGGCCAGGCC TGTGCCCGTC CCCGCCCGGG 
GAAACGAAGG CTGCCAGTCT GCTCCTGGAA AGCCCTCTGC CTTTGGCCCA GAGACTGAAA 
CTGCGATTCC AGAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEN1 ( XM_031949604.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031805464.1 Sarcophilus harrisii GEN1 Holliday junction 5' flap endonuclease (GEN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa223467
Clone ID Related Accession (Same CDS sequence)	XM_031949603.1 , XM_031949604.1
Accession Version	XM_031949604.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	flap endonuclease GEN homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTGA ATGATCTGTG GCAGATTCTG GAGCCTGTTA AGCGGCACGC CCCTCTCCGG 
AGTCTCCAGG GGAAGACCCT GGCCGTTGAC CTGAGTCTCT GGGTATGCGA AGCACTTGCT 
GTCAAAAAGA TGGTCGGCAT CGTTGTCAAG CCCCACCTCA GGAACCTGTT TTTTCGCATC 
TCATCTCTAA CCTTAATGGA AGTAAAACTG CTCTTCGTCA TGGAAGGCGA CGCTCCCAAG 
CTGAAAGCAG ACGTGATGGG CAAACGGAAC GAGACGCGCT TCGGGGCCCC CCAAAACCGG 
GCCCACAAAT TCACAAGATC CTCTTTCAAG GCTGTCTTAA AAGAGTGCCT TGACTTGTTG 
GAGTGCTTGG GAGTTCCGTG GGTTCAGGCA TCCGGGGAAG CCGAGGCTAT GTGCGCCTAC 
CTCGATGCCC ATGGCTATGT GGACGGGTGC CTCACTGACG ATGGAGACGC TTTCCTTTAC 
GGAGCACAGA CTGTTTATCG AAATTTCACT ATGAATGCAA AGGACCCCCA TGTGGATTGC 
TACACGATGT CCTCGATTAA GCGGGATCTG GGCCTGGATC GGGACACCCT GATTGGCCTG 
GCCGTGCTGC TGGGCTGTGA CTACCTTCCG AAGGGAGTCC CTGGAGTTGG AAAGGAGCTG 
GCGCTAAAGC TTGTGCGCTC TTTGAAAGGG CAGAACTTGC TGCAGAAGTT CGATCAGTGG 
AAGGAGGAGT CCCACAGCCC CGACCTGCCG CCAGCCCCAG TTAGGAAGCC AGCTCATTGC 
AATGTGTGCG CCCATCCAGG CTCCCCCGGG GACCACGAGC TGCGCGGGTG CCGCCTGTGC 
GGCAGCGAGC GCTACTGCGA GCCCCACGAC CCCAAGTACC GCTGCCTGTG CGACTGGCAC 
CGCGCCGAGC ACCGCCGGCT ACAGGGTGCT GCCGAGAACA GCGTCAAGAA GAAAGCTTGC 
GCTTGCCAAG GATTCCCGTT CCCTGAGGTC ATTCGAGAAT TCCAGGTAAA CAAGGATCGA 
GTGACCAAGA AAATCTGCTT CCGGAGACCT GACCTCTTGT CCTTCCAGAT TTTTGCCTTG 
GAGAAGCTGG AGTGGCCGCG GCACTACGCC TGCGAGAAGC TCATGGGGCT CTTGGCCCAT 
CACGACATGG TGGAACGGAA GCTTGGGCAC AAGGGCCCCG CCCAGCTGCG GCCCCTGCGA 
ATAACCCGCT CCCGCCTCAG GAGCGGCGTC CCTTGCTTGG AGATCCTGTG GCAGAAGCCA 
GAGCACTACG TAATGGCCAG CGCGGCTCCC CAGGCCGGGC CACTCGCCGT GCTCACGGTG 
GAGGAAGCCG CCCTGTTTGA AGCTGCCTAC CCCGAGGTGG TCGCCCAGTA CCAGGAGCAG 
CTGCAGGAAA ACCAGGGGAA GAAGTCCAAG AGTAAGAAGA ACAAGGACAA GGGGGGCGAT 
CTGCTGGATG TGGGCGAGGT GACCGGCCTT CTGGCCCACG TGAGACTCTC CCACGAGATG 
GGCCCGGGAC AGGCCGCCAA GGCAGCCCTG AAAGCGCCCC CAGAGGAGGA ATGGCCGCAG 
AGAAACAGCC CCGTGGGGGG CAGCCGGCTT TGGAGTGGGG CTCCCCTAAG TCTGGAAGCC 
TGGCAGCTGC CCGCCCCCAC CCCCACCCCC GCTTCCACCC CCACCCCCCT GAGACTCGGG 
GGCTCACAGC ACGGTTGGGC CAGCAGCCAT CTGCTGTCCA TGTCCGAGCT CCAGGACGAT 
AGCTCGGCCA TCGAGGCCCT CCAGCTGAGC ACCATCGACT GGGAGGGCAC CTCTTTCAGC 
GTCTCCCCGG GGGCCCACTC CTCAGGGCCC AGCGGCGACA GCCCCCACGA GCCCCAGAGC 
ACCGGCTCGG CCAGAACCCA TTCGAGCCTC GGCTCGACCC TTCAGCCTCT GAAGGAGTGG 
GGGTGTGCCC AGCCACTGGC GCAGCCCCAG CTCTCGGCTC CGGACGGGCG CCACGGCCAC 
TGGGCCGACC CGGAAGCCTC GGCGCTGCAG AGGAAAGGGG CCTTCACTGC GAAAAGCGGC 
CCCTCCTTCG AGCCCCAGCA GCCTAACTGT AGCCCCCCAG GTCTTTTCCC AGTGTTCCGT 
TTGCAGAGCA GCCTGGACCA GTCTGACATG GCAAGCAGGA GGGCACCCAG AGCTGGAGCG 
TTTGCGCCCC TTGGCTCCGG CAATCCAACG CTGAGACCCC TGGGCCGGGG GCTCCCCCGA 
CTTCATCCCG AGGCTCAGGC TACTCCGAAG AGATTCCCCA AAAAGACCGT CTGCCTCCCC 
TGCAGCTCCT CAGAGGAGGA GGACGAGCTG ACTAGCAGGA GCGAGAAGTG CCTGCGGCCG 
AAGGGGAAGC CCGAGCAGCG CCCTCCGGCC TGGCTCCAGA CCAGTGTCCC GCGGCCAGAT 
CCCAGAACCC TCTGTAAGGG AGCCGGGGCT CCATGCTCGG CCACGGCCAC CTCCACATTC 
GACCTCCTGG ACGCTGCGGG GAGCTCGCTG CGGCCAGGCC TGTGCCCGTC CCCGCCCGGG 
GAAACGAAGG CTGCCAGTCT GCTCCTGGAA AGCCCTCTGC CTTTGGCCCA GAGACTGAAA 
CTGCGATTCC AGAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031805464.1
CDS	796..3396
Translation	MGVNDLWQILEPVKRHAPLRSLQGKTLAVDLSLWVCEALAVKKMVGIVVKPHLRNLFFRISSLTLMEVKLLFVMEGDAPKLKADVMGKRNETRFGAPQNRAHKFTRSSFKAVLKECLDLLECLGVPWVQASGEAEAMCAYLDAHGYVDGCLTDDGDAFLYGAQTVYRNFTMNAKDPHVDCYTMSSIKRDLGLDRDTLIGLAVLLGCDYLPKGVPGVGKELALKLVRSLKGQNLLQKFDQWKEESHSPDLPPAPVRKPAHCNVCAHPGSPGDHELRGCRLCGSERYCEPHDPKYRCLCDWHRAEHRRLQGAAENSVKKKACACQGFPFPEVIREFQVNKDRVTKKICFRRPDLLSFQIFALEKLEWPRHYACEKLMGLLAHHDMVERKLGHKGPAQLRPLRITRSRLRSGVPCLEILWQKPEHYVMASAAPQAGPLAVLTVEEAALFEAAYPEVVAQYQEQLQENQGKKSKSKKNKDKGGDLLDVGEVTGLLAHVRLSHEMGPGQAAKAALKAPPEEEWPQRNSPVGGSRLWSGAPLSLEAWQLPAPTPTPASTPTPLRLGGSQHGWASSHLLSMSELQDDSSAIEALQLSTIDWEGTSFSVSPGAHSSGPSGDSPHEPQSTGSARTHSSLGSTLQPLKEWGCAQPLAQPQLSAPDGRHGHWADPEASALQRKGAFTAKSGPSFEPQQPNCSPPGLFPVFRLQSSLDQSDMASRRAPRAGAFAPLGSGNPTLRPLGRGLPRLHPEAQATPKRFPKKTVCLPCSSSEEEDELTSRSEKCLRPKGKPEQRPPAWLQTSVPRPDPRTLCKGAGAPCSATATSTFDLLDAAGSSLRPGLCPSPPGETKAASLLLESPLPLAQRLKLRFQSS

Target ORF information:

RefSeq Version	XM_031949604.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii GEN1 Holliday junction 5' flap endonuclease (GEN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031949604.1

ORF Insert Sequence:

ATGGGGGTGA ATGATCTGTG GCAGATTCTG GAGCCTGTTA AGCGGCACGC CCCTCTCCGG 
AGTCTCCAGG GGAAGACCCT GGCCGTTGAC CTGAGTCTCT GGGTATGCGA AGCACTTGCT 
GTCAAAAAGA TGGTCGGCAT CGTTGTCAAG CCCCACCTCA GGAACCTGTT TTTTCGCATC 
TCATCTCTAA CCTTAATGGA AGTAAAACTG CTCTTCGTCA TGGAAGGCGA CGCTCCCAAG 
CTGAAAGCAG ACGTGATGGG CAAACGGAAC GAGACGCGCT TCGGGGCCCC CCAAAACCGG 
GCCCACAAAT TCACAAGATC CTCTTTCAAG GCTGTCTTAA AAGAGTGCCT TGACTTGTTG 
GAGTGCTTGG GAGTTCCGTG GGTTCAGGCA TCCGGGGAAG CCGAGGCTAT GTGCGCCTAC 
CTCGATGCCC ATGGCTATGT GGACGGGTGC CTCACTGACG ATGGAGACGC TTTCCTTTAC 
GGAGCACAGA CTGTTTATCG AAATTTCACT ATGAATGCAA AGGACCCCCA TGTGGATTGC 
TACACGATGT CCTCGATTAA GCGGGATCTG GGCCTGGATC GGGACACCCT GATTGGCCTG 
GCCGTGCTGC TGGGCTGTGA CTACCTTCCG AAGGGAGTCC CTGGAGTTGG AAAGGAGCTG 
GCGCTAAAGC TTGTGCGCTC TTTGAAAGGG CAGAACTTGC TGCAGAAGTT CGATCAGTGG 
AAGGAGGAGT CCCACAGCCC CGACCTGCCG CCAGCCCCAG TTAGGAAGCC AGCTCATTGC 
AATGTGTGCG CCCATCCAGG CTCCCCCGGG GACCACGAGC TGCGCGGGTG CCGCCTGTGC 
GGCAGCGAGC GCTACTGCGA GCCCCACGAC CCCAAGTACC GCTGCCTGTG CGACTGGCAC 
CGCGCCGAGC ACCGCCGGCT ACAGGGTGCT GCCGAGAACA GCGTCAAGAA GAAAGCTTGC 
GCTTGCCAAG GATTCCCGTT CCCTGAGGTC ATTCGAGAAT TCCAGGTAAA CAAGGATCGA 
GTGACCAAGA AAATCTGCTT CCGGAGACCT GACCTCTTGT CCTTCCAGAT TTTTGCCTTG 
GAGAAGCTGG AGTGGCCGCG GCACTACGCC TGCGAGAAGC TCATGGGGCT CTTGGCCCAT 
CACGACATGG TGGAACGGAA GCTTGGGCAC AAGGGCCCCG CCCAGCTGCG GCCCCTGCGA 
ATAACCCGCT CCCGCCTCAG GAGCGGCGTC CCTTGCTTGG AGATCCTGTG GCAGAAGCCA 
GAGCACTACG TAATGGCCAG CGCGGCTCCC CAGGCCGGGC CACTCGCCGT GCTCACGGTG 
GAGGAAGCCG CCCTGTTTGA AGCTGCCTAC CCCGAGGTGG TCGCCCAGTA CCAGGAGCAG 
CTGCAGGAAA ACCAGGGGAA GAAGTCCAAG AGTAAGAAGA ACAAGGACAA GGGGGGCGAT 
CTGCTGGATG TGGGCGAGGT GACCGGCCTT CTGGCCCACG TGAGACTCTC CCACGAGATG 
GGCCCGGGAC AGGCCGCCAA GGCAGCCCTG AAAGCGCCCC CAGAGGAGGA ATGGCCGCAG 
AGAAACAGCC CCGTGGGGGG CAGCCGGCTT TGGAGTGGGG CTCCCCTAAG TCTGGAAGCC 
TGGCAGCTGC CCGCCCCCAC CCCCACCCCC GCTTCCACCC CCACCCCCCT GAGACTCGGG 
GGCTCACAGC ACGGTTGGGC CAGCAGCCAT CTGCTGTCCA TGTCCGAGCT CCAGGACGAT 
AGCTCGGCCA TCGAGGCCCT CCAGCTGAGC ACCATCGACT GGGAGGGCAC CTCTTTCAGC 
GTCTCCCCGG GGGCCCACTC CTCAGGGCCC AGCGGCGACA GCCCCCACGA GCCCCAGAGC 
ACCGGCTCGG CCAGAACCCA TTCGAGCCTC GGCTCGACCC TTCAGCCTCT GAAGGAGTGG 
GGGTGTGCCC AGCCACTGGC GCAGCCCCAG CTCTCGGCTC CGGACGGGCG CCACGGCCAC 
TGGGCCGACC CGGAAGCCTC GGCGCTGCAG AGGAAAGGGG CCTTCACTGC GAAAAGCGGC 
CCCTCCTTCG AGCCCCAGCA GCCTAACTGT AGCCCCCCAG GTCTTTTCCC AGTGTTCCGT 
TTGCAGAGCA GCCTGGACCA GTCTGACATG GCAAGCAGGA GGGCACCCAG AGCTGGAGCG 
TTTGCGCCCC TTGGCTCCGG CAATCCAACG CTGAGACCCC TGGGCCGGGG GCTCCCCCGA 
CTTCATCCCG AGGCTCAGGC TACTCCGAAG AGATTCCCCA AAAAGACCGT CTGCCTCCCC 
TGCAGCTCCT CAGAGGAGGA GGACGAGCTG ACTAGCAGGA GCGAGAAGTG CCTGCGGCCG 
AAGGGGAAGC CCGAGCAGCG CCCTCCGGCC TGGCTCCAGA CCAGTGTCCC GCGGCCAGAT 
CCCAGAACCC TCTGTAAGGG AGCCGGGGCT CCATGCTCGG CCACGGCCAC CTCCACATTC 
GACCTCCTGG ACGCTGCGGG GAGCTCGCTG CGGCCAGGCC TGTGCCCGTC CCCGCCCGGG 
GAAACGAAGG CTGCCAGTCT GCTCCTGGAA AGCCCTCTGC CTTTGGCCCA GAGACTGAAA 
CTGCGATTCC AGAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GEN1 ( XM_031949605.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031805465.1 Sarcophilus harrisii GEN1 Holliday junction 5' flap endonuclease (GEN1), transcript variant X3, mRNA.

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CloneID	OSa223468
Clone ID Related Accession (Same CDS sequence)	XM_031949605.1
Accession Version	XM_031949605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	flap endonuclease GEN homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCTG GCCAGCACCA CGTCAAGCCC CACCTCAGGA ACCTGTTTTT TCGCATCTCA 
TCTCTAACCT TAATGGAAGT AAAACTGCTC TTCGTCATGG AAGGCGACGC TCCCAAGCTG 
AAAGCAGACG TGATGGGCAA ACGGAACGAG ACGCGCTTCG GGGCCCCCCA AAACCGGGCC 
CACAAATTCA CAAGATCCTC TTTCAAGGCT GTCTTAAAAG AGTGCCTTGA CTTGTTGGAG 
TGCTTGGGAG TTCCGTGGGT TCAGGCATCC GGGGAAGCCG AGGCTATGTG CGCCTACCTC 
GATGCCCATG GCTATGTGGA CGGGTGCCTC ACTGACGATG GAGACGCTTT CCTTTACGGA 
GCACAGACTG TTTATCGAAA TTTCACTATG AATGCAAAGG ACCCCCATGT GGATTGCTAC 
ACGATGTCCT CGATTAAGCG GGATCTGGGC CTGGATCGGG ACACCCTGAT TGGCCTGGCC 
GTGCTGCTGG GCTGTGACTA CCTTCCGAAG GGAGTCCCTG GAGTTGGAAA GGAGCTGGCG 
CTAAAGCTTG TGCGCTCTTT GAAAGGGCAG AACTTGCTGC AGAAGTTCGA TCAGTGGAAG 
GAGGAGTCCC ACAGCCCCGA CCTGCCGCCA GCCCCAGTTA GGAAGCCAGC TCATTGCAAT 
GTGTGCGCCC ATCCAGGCTC CCCCGGGGAC CACGAGCTGC GCGGGTGCCG CCTGTGCGGC 
AGCGAGCGCT ACTGCGAGCC CCACGACCCC AAGTACCGCT GCCTGTGCGA CTGGCACCGC 
GCCGAGCACC GCCGGCTACA GGGTGCTGCC GAGAACAGCG TCAAGAAGAA AGCTTGCGCT 
TGCCAAGGAT TCCCGTTCCC TGAGGTCATT CGAGAATTCC AGGTAAACAA GGATCGAGTG 
ACCAAGAAAA TCTGCTTCCG GAGACCTGAC CTCTTGTCCT TCCAGATTTT TGCCTTGGAG 
AAGCTGGAGT GGCCGCGGCA CTACGCCTGC GAGAAGCTCA TGGGGCTCTT GGCCCATCAC 
GACATGGTGG AACGGAAGCT TGGGCACAAG GGCCCCGCCC AGCTGCGGCC CCTGCGAATA 
ACCCGCTCCC GCCTCAGGAG CGGCGTCCCT TGCTTGGAGA TCCTGTGGCA GAAGCCAGAG 
CACTACGTAA TGGCCAGCGC GGCTCCCCAG GCCGGGCCAC TCGCCGTGCT CACGGTGGAG 
GAAGCCGCCC TGTTTGAAGC TGCCTACCCC GAGGTGGTCG CCCAGTACCA GGAGCAGCTG 
CAGGAAAACC AGGGGAAGAA GTCCAAGAGT AAGAAGAACA AGGACAAGGG GGGCGATCTG 
CTGGATGTGG GCGAGGTGAC CGGCCTTCTG GCCCACGTGA GACTCTCCCA CGAGATGGGC 
CCGGGACAGG CCGCCAAGGC AGCCCTGAAA GCGCCCCCAG AGGAGGAATG GCCGCAGAGA 
AACAGCCCCG TGGGGGGCAG CCGGCTTTGG AGTGGGGCTC CCCTAAGTCT GGAAGCCTGG 
CAGCTGCCCG CCCCCACCCC CACCCCCGCT TCCACCCCCA CCCCCCTGAG ACTCGGGGGC 
TCACAGCACG GTTGGGCCAG CAGCCATCTG CTGTCCATGT CCGAGCTCCA GGACGATAGC 
TCGGCCATCG AGGCCCTCCA GCTGAGCACC ATCGACTGGG AGGGCACCTC TTTCAGCGTC 
TCCCCGGGGG CCCACTCCTC AGGGCCCAGC GGCGACAGCC CCCACGAGCC CCAGAGCACC 
GGCTCGGCCA GAACCCATTC GAGCCTCGGC TCGACCCTTC AGCCTCTGAA GGAGTGGGGG 
TGTGCCCAGC CACTGGCGCA GCCCCAGCTC TCGGCTCCGG ACGGGCGCCA CGGCCACTGG 
GCCGACCCGG AAGCCTCGGC GCTGCAGAGG AAAGGGGCCT TCACTGCGAA AAGCGGCCCC 
TCCTTCGAGC CCCAGCAGCC TAACTGTAGC CCCCCAGGTC TTTTCCCAGT GTTCCGTTTG 
CAGAGCAGCC TGGACCAGTC TGACATGGCA AGCAGGAGGG CACCCAGAGC TGGAGCGTTT 
GCGCCCCTTG GCTCCGGCAA TCCAACGCTG AGACCCCTGG GCCGGGGGCT CCCCCGACTT 
CATCCCGAGG CTCAGGCTAC TCCGAAGAGA TTCCCCAAAA AGACCGTCTG CCTCCCCTGC 
AGCTCCTCAG AGGAGGAGGA CGAGCTGACT AGCAGGAGCG AGAAGTGCCT GCGGCCGAAG 
GGGAAGCCCG AGCAGCGCCC TCCGGCCTGG CTCCAGACCA GTGTCCCGCG GCCAGATCCC 
AGAACCCTCT GTAAGGGAGC CGGGGCTCCA TGCTCGGCCA CGGCCACCTC CACATTCGAC 
CTCCTGGACG CTGCGGGGAG CTCGCTGCGG CCAGGCCTGT GCCCGTCCCC GCCCGGGGAA 
ACGAAGGCTG CCAGTCTGCT CCTGGAAAGC CCTCTGCCTT TGGCCCAGAG ACTGAAACTG 
CGATTCCAGA GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031805465.1
CDS	130..2607
Translation	MKAGQHHVKPHLRNLFFRISSLTLMEVKLLFVMEGDAPKLKADVMGKRNETRFGAPQNRAHKFTRSSFKAVLKECLDLLECLGVPWVQASGEAEAMCAYLDAHGYVDGCLTDDGDAFLYGAQTVYRNFTMNAKDPHVDCYTMSSIKRDLGLDRDTLIGLAVLLGCDYLPKGVPGVGKELALKLVRSLKGQNLLQKFDQWKEESHSPDLPPAPVRKPAHCNVCAHPGSPGDHELRGCRLCGSERYCEPHDPKYRCLCDWHRAEHRRLQGAAENSVKKKACACQGFPFPEVIREFQVNKDRVTKKICFRRPDLLSFQIFALEKLEWPRHYACEKLMGLLAHHDMVERKLGHKGPAQLRPLRITRSRLRSGVPCLEILWQKPEHYVMASAAPQAGPLAVLTVEEAALFEAAYPEVVAQYQEQLQENQGKKSKSKKNKDKGGDLLDVGEVTGLLAHVRLSHEMGPGQAAKAALKAPPEEEWPQRNSPVGGSRLWSGAPLSLEAWQLPAPTPTPASTPTPLRLGGSQHGWASSHLLSMSELQDDSSAIEALQLSTIDWEGTSFSVSPGAHSSGPSGDSPHEPQSTGSARTHSSLGSTLQPLKEWGCAQPLAQPQLSAPDGRHGHWADPEASALQRKGAFTAKSGPSFEPQQPNCSPPGLFPVFRLQSSLDQSDMASRRAPRAGAFAPLGSGNPTLRPLGRGLPRLHPEAQATPKRFPKKTVCLPCSSSEEEDELTSRSEKCLRPKGKPEQRPPAWLQTSVPRPDPRTLCKGAGAPCSATATSTFDLLDAAGSSLRPGLCPSPPGETKAASLLLESPLPLAQRLKLRFQSS

Target ORF information:

RefSeq Version	XM_031949605.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii GEN1 Holliday junction 5' flap endonuclease (GEN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031949605.1

ORF Insert Sequence:

ATGAAAGCTG GCCAGCACCA CGTCAAGCCC CACCTCAGGA ACCTGTTTTT TCGCATCTCA 
TCTCTAACCT TAATGGAAGT AAAACTGCTC TTCGTCATGG AAGGCGACGC TCCCAAGCTG 
AAAGCAGACG TGATGGGCAA ACGGAACGAG ACGCGCTTCG GGGCCCCCCA AAACCGGGCC 
CACAAATTCA CAAGATCCTC TTTCAAGGCT GTCTTAAAAG AGTGCCTTGA CTTGTTGGAG 
TGCTTGGGAG TTCCGTGGGT TCAGGCATCC GGGGAAGCCG AGGCTATGTG CGCCTACCTC 
GATGCCCATG GCTATGTGGA CGGGTGCCTC ACTGACGATG GAGACGCTTT CCTTTACGGA 
GCACAGACTG TTTATCGAAA TTTCACTATG AATGCAAAGG ACCCCCATGT GGATTGCTAC 
ACGATGTCCT CGATTAAGCG GGATCTGGGC CTGGATCGGG ACACCCTGAT TGGCCTGGCC 
GTGCTGCTGG GCTGTGACTA CCTTCCGAAG GGAGTCCCTG GAGTTGGAAA GGAGCTGGCG 
CTAAAGCTTG TGCGCTCTTT GAAAGGGCAG AACTTGCTGC AGAAGTTCGA TCAGTGGAAG 
GAGGAGTCCC ACAGCCCCGA CCTGCCGCCA GCCCCAGTTA GGAAGCCAGC TCATTGCAAT 
GTGTGCGCCC ATCCAGGCTC CCCCGGGGAC CACGAGCTGC GCGGGTGCCG CCTGTGCGGC 
AGCGAGCGCT ACTGCGAGCC CCACGACCCC AAGTACCGCT GCCTGTGCGA CTGGCACCGC 
GCCGAGCACC GCCGGCTACA GGGTGCTGCC GAGAACAGCG TCAAGAAGAA AGCTTGCGCT 
TGCCAAGGAT TCCCGTTCCC TGAGGTCATT CGAGAATTCC AGGTAAACAA GGATCGAGTG 
ACCAAGAAAA TCTGCTTCCG GAGACCTGAC CTCTTGTCCT TCCAGATTTT TGCCTTGGAG 
AAGCTGGAGT GGCCGCGGCA CTACGCCTGC GAGAAGCTCA TGGGGCTCTT GGCCCATCAC 
GACATGGTGG AACGGAAGCT TGGGCACAAG GGCCCCGCCC AGCTGCGGCC CCTGCGAATA 
ACCCGCTCCC GCCTCAGGAG CGGCGTCCCT TGCTTGGAGA TCCTGTGGCA GAAGCCAGAG 
CACTACGTAA TGGCCAGCGC GGCTCCCCAG GCCGGGCCAC TCGCCGTGCT CACGGTGGAG 
GAAGCCGCCC TGTTTGAAGC TGCCTACCCC GAGGTGGTCG CCCAGTACCA GGAGCAGCTG 
CAGGAAAACC AGGGGAAGAA GTCCAAGAGT AAGAAGAACA AGGACAAGGG GGGCGATCTG 
CTGGATGTGG GCGAGGTGAC CGGCCTTCTG GCCCACGTGA GACTCTCCCA CGAGATGGGC 
CCGGGACAGG CCGCCAAGGC AGCCCTGAAA GCGCCCCCAG AGGAGGAATG GCCGCAGAGA 
AACAGCCCCG TGGGGGGCAG CCGGCTTTGG AGTGGGGCTC CCCTAAGTCT GGAAGCCTGG 
CAGCTGCCCG CCCCCACCCC CACCCCCGCT TCCACCCCCA CCCCCCTGAG ACTCGGGGGC 
TCACAGCACG GTTGGGCCAG CAGCCATCTG CTGTCCATGT CCGAGCTCCA GGACGATAGC 
TCGGCCATCG AGGCCCTCCA GCTGAGCACC ATCGACTGGG AGGGCACCTC TTTCAGCGTC 
TCCCCGGGGG CCCACTCCTC AGGGCCCAGC GGCGACAGCC CCCACGAGCC CCAGAGCACC 
GGCTCGGCCA GAACCCATTC GAGCCTCGGC TCGACCCTTC AGCCTCTGAA GGAGTGGGGG 
TGTGCCCAGC CACTGGCGCA GCCCCAGCTC TCGGCTCCGG ACGGGCGCCA CGGCCACTGG 
GCCGACCCGG AAGCCTCGGC GCTGCAGAGG AAAGGGGCCT TCACTGCGAA AAGCGGCCCC 
TCCTTCGAGC CCCAGCAGCC TAACTGTAGC CCCCCAGGTC TTTTCCCAGT GTTCCGTTTG 
CAGAGCAGCC TGGACCAGTC TGACATGGCA AGCAGGAGGG CACCCAGAGC TGGAGCGTTT 
GCGCCCCTTG GCTCCGGCAA TCCAACGCTG AGACCCCTGG GCCGGGGGCT CCCCCGACTT 
CATCCCGAGG CTCAGGCTAC TCCGAAGAGA TTCCCCAAAA AGACCGTCTG CCTCCCCTGC 
AGCTCCTCAG AGGAGGAGGA CGAGCTGACT AGCAGGAGCG AGAAGTGCCT GCGGCCGAAG 
GGGAAGCCCG AGCAGCGCCC TCCGGCCTGG CTCCAGACCA GTGTCCCGCG GCCAGATCCC 
AGAACCCTCT GTAAGGGAGC CGGGGCTCCA TGCTCGGCCA CGGCCACCTC CACATTCGAC 
CTCCTGGACG CTGCGGGGAG CTCGCTGCGG CCAGGCCTGT GCCCGTCCCC GCCCGGGGAA 
ACGAAGGCTG CCAGTCTGCT CCTGGAAAGC CCTCTGCCTT TGGCCCAGAG ACTGAAACTG 
CGATTCCAGA GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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GEN1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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