HELZ2 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	HELZ2
Entrez Gene ID	100915383
Full Name	helicase with zinc finger 2
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012554098.2	XP_012409552.1	helicase with zinc finger domain 2 isoform X1
XM_012554098.3	XP_012409552.1	helicase with zinc finger domain 2 isoform X1
XM_012554098.1	XP_012409552.1	helicase with zinc finger domain 2
XM_023494447.1	XP_023350215.1	helicase with zinc finger domain 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following HELZ2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HELZ2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa150475	XM_023494447.1 Latest version!	Sarcophilus harrisii helicase with zinc finger 2 (HELZ2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa119203	XM_012554098.3 Latest version!	Sarcophilus harrisii helicase with zinc finger 2 (HELZ2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OSa119203	XM_012554098.2	Sarcophilus harrisii helicase with zinc finger 2 (HELZ2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa119203	XM_012554098.1	Sarcophilus harrisii helicase with zinc finger 2, transcriptional coactivator (HELZ2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HELZ2 ( XM_023494447.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023350215.1 Sarcophilus harrisii helicase with zinc finger 2 (HELZ2), transcript variant X2, mRNA.

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CloneID	OSa150475
Clone ID Related Accession (Same CDS sequence)	XM_023494447.1
Accession Version	XM_023494447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	helicase with zinc finger domain 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCT CTCCTACCAG GACCGGCTTT TACATGAATA TCAACACTGC CACAATGAAA 
TACAGGTGCT TCCTGTGCCC ATCTGCCACT CAGATGTCCG AGTCTATACG TGATGTAACT 
ATTCACTGTG ACCAGCCCTT AAAATGCCAG GTAGAGGACA AGAAATCGCA ATGCATATGG 
AAATTTATCA TCCATTCTAA GGACCCACTG CAGCACGTGG CCCTCCTCAA ACATCAGATG 
GGAGCCATCT TTTCCTTGGT GTGTCCTGGG CTTCCACAAA GACAGCTTTA TGCCCAAGGC 
TCAAGGTTCC ATGTGCAGGA TACAGCGATG ACCTTCCAGG TGGAGGTGCT GGTAGAGTGT 
ATCACCTTTG GCAGCTATGA ACAATGGGTA GCTTTTGACT TTGGACGCCG ACCTGTCCTG 
GTACAGAAGC TATATGTTCA AGTGGGCCAA AAGGAGACCC TTGGGCCTCC CCCCTGTATC 
CAGGACCATA ATTGTCTTCT TGAGTTGATC CGCTGGCACA GTGGAAATCG ATGTGTGGTG 
CCCAATGTGG GCAGGACAGC TGAGCAGGTG AATCTGCTAG CTAAGTACAA AGTGAGCCCC 
CAGGAACTGG AGAACTACAG GTCAAAGGCT TCAGACCAGG TCTCTATAAC TCCAGTCAAC 
TATCGGGAGA GGATGCACCA ATTCTTGTAT ATGGAAGAGG AGGCTCAACA GGAGCTGGTG 
GCCAAATTGA ACCTTCAAGT CGAAGTCACA TTGACCTCCA AGATGCAGAC CTTGGCAATG 
GGCATGAAGT TTGCACCATC TGGGGAATTG TATGCTAAGG TTCCAATTCC TTGTTCCCTG 
ACACCAGACA CAGACCAGGG CTATTTGCTA AGTAGAGCTG TTAGCACTGC TTACGTGGCA 
CCTGTCCCTG CCCCTGACAA TCATGTGTAT GAGGTTCGGG TGGAAGTTAA AGCCACTTCA 
GAGCAGAATG TGTGGCTCCT ACTGCCAGCC CACTGCTGCT CTGCCTTGGG ACTTAAGGAT 
GAAGACACAC AGCTTATGGA AATCCAGTTT CAAATCGACA AGACTATATT TGGGATATGG 
CACCAAGCCA TTGATGCACT TCAGGATGAT AGACTGGTGG CACCTGATGT ACCTGCCTGT 
TCTCTGCCTT CTTTGAGGCC CCCTCCCCAG GAACTCCGTG GTAATCCCAA GCAGAAGGAG 
GCCATCTCCT TTATCACAGG GACAACGAGT GGTCAGAGCC GAGTGCCTCC ACTGCTCATT 
TATGGCCCCT TTGGGACAGG CAAGACATAC ACACTAGCTA TGGCCACGCT AGAGGTTATC 
AAACAGCCAA ACACAAAAGT GTTGATCTGT ACTCACACCA ACAGTGCTGC TGACATCTAC 
ATCCGTGAGT ACTTTCATGC TTATGTCACA GCTGGACACC CTGAGGCTGT GCCATTGAGG 
GTCAAAAACA CTGAAAGCTT CCTCAACCAG ACAGACCCGA CCACTCTGCA GTATTGCTGC 
CTCTCAAGTG ATGGACGGTC CTTTTGCTTC CCAACTCAGG AGAAACTAAA GCAGCACCGC 
ATTATAATCA CTACCACTAC CTTGTCCCGC TCCCTATGTG TGCCATCTGG GTTTTTTTCC 
CACATTCTCA TTGATGAGGC TGCTCAGATG TTAGAATGTG AAGCCATCAT CCCACTGGCC 
TATGCTACCC AGAAGACACG CATTGTCCTT GCCGGGGACC ACATGCAAAT CACCCCCAAA 
CTCTTCAGTG TTGGGGCCAG GAAGTCTGCT GACCATACAC TGCTTAATCG GCTGTTCCAG 
TACTATCAGA AGGAGACTCA TAAATTGGCT TCCCAGAGCA GGGTCATCTT CCATGAAAAC 
TACCGTTCCA CCAAAGCCAT CATTTCCTTT GTGTCTCGGA ATTTCTATGT GGCTAAGGGT 
AACCCTATTC AGGCTAGCGG ACAGATCCCT GCACACCCTT GCAAGTACCC ACTCATGTTT 
TGCCATGTGG CTGGTACTCC TGAGAGAGAC ATAACAATGA CATCATGGTT TAACAGGGCT 
GAGATAGTGG AGGTGATTGA GAAGGTGCAA GAATTCTATC GTATCTGGGC CTACCAATGG 
AATAGCCCTG ACCTAAAGCA AATTTGTGTT GTGTCCCATG GTTCTCAGGT AAAAATATTG 
AGGCAGGAAC TGAGGAAGAA ACAACTAGGA GAAGTAGCAG TGGAGAATTT TGAAAATCTG 
CCAGGTCGTG AATTCCGAGT GATCATCCTC AGCACAGTCC ACACTCGGGA CAGCCTGCTC 
AACAGCTCAG TCCCCCACCT AGAATTCTTC AGTGAGGCTC GGGTGCTGAA CACCGTGATG 
ACCAGAGCCC AGTCCCAGGT GGTTGCTGTG GGCGATGCTG TGGCCCTTTG TTCTTTTGGA 
CGTTGTAGCA AGGTATGGAA GCACTTCATC CAAGAGTGTA TAGAGATGCG CAGTGTGTCC 
CCTGTGGACC TCACAATGGA ACAAATCAAG CAAGATGTGG CACACAGGCA GCGCTGGGCC 
CGGCAAAATG GACTGCATCC TATGAACTCT CCCAGGGAGC ACTCGTTAGA AGAGGAAGAC 
AATGACAATG ACATTCCCTC ATGGGTGTCT GATGCTGAGC TCAATGCTGA TGATCCCATC 
CTCCAGGAGT TGCTGGATGA GAGTAAGAAT GTGGCAGTGA CAGTAAGTGA GGATGGGCTG 
CTGAATGTGA CATCAGGGTC CCGGTCCCGC TATCAGGGAC AACAGTATTT AAGCTTTCCT 
GTGCCAGTGT TGCAGAAATA TCTCCGAATG CAACCCAAGA TATATAGGAA ATGTGAACTG 
ATAAAGGAGG CCTTTGAGAA GGCATCTGCC TTCACCATAG ATGACTCCCC ACCCCTCATT 
ATTCAGATCA GAGGACGCAC CAACTGTGGA ATGGCCTTCA CTGGGGATCA AGTTCTAGTG 
GAAATCCTGA CTCCAGAATT GGAGGAACAG GGGTACTCCT GGCGCCCCAT GGGCCGAGTG 
GTAGGAATAC TAGAAAGAGG TGACCGTGAA CTCATCTTCA TTTGCAAAAT GGATGAGTTT 
GACCCCCGAG TTATGATCCC CATTGACCCG TCAGTCACCA AGATCTTTGT GCCAGAGTTA 
AAGGATAAGC CCAACACGAT TCCTATCCGC CGCCTACAAC GAGGACACAT CAAGCTTATT 
AGCTATGAGA AGATAACAGA GGAGACCAAG CGTAATCAGC TCTTTCGTGT TCAGATCATC 
TCTTGGAAAG AGAGGTTCTA TTATCCCTTA GGCATCATTC TTGAGATTCT TCCCTTGCCT 
TTGACCTTAG AACAAGGTTT AAAGATTCTG GACATGGAAT ATTCTCTGAA TGCTAATGGA 
AGATACCCAA CTCCAGTAAT TAAGGAAGTG AACCAACACA ACTGTAGTAA ATTGAATCTG 
CACCGGGGAC AGTGGAAGGA CTGCCGGGAT TATTTGACCT TTACTGTGGA CCCGCAGGGC 
TCCCGGGATC TGGATGATGC TATCAGTGTC CGGGACCTGG GAAGCCATTA TGAAATTGCT 
GTCCATATAT CTGATGTGGC TAGCTTCATG CCAAAGGATG GTGCACTTGA CCGAGAGGCT 
AGAAAGCGAG GTGCTACCTA CTATGCTCCT GGAAAGGATC CAGTGGCCAT GCTCCCTTCC 
AGGCTCAGCC AGGACCTGTT CAGTCTCCTT CCCCAGCAAG AACGTCGCAC TATCTCCCTG 
ATTCTTACAG TGGAGAAGGA AAGTGACCGA GTAGTGCAAT GGCATATCAC ACCATCTATG 
ATATGCTCTG ACCTCCAACT GACGTATGAG GAGGCTGAGG TTATTATTAA GAGTCATCCT 
GGGGGTGGGG CCTGGCTGCC CCTCCGCCTG GGCACTGTAG AAGGCTGTGT TGTGGCTGCT 
TATCACTTCT CCCAAGTCCT CCGCAGAAGC CGCCTTCAGG GAGATTGCTA CTATGACCAA 
CCAGATGAAG ACAGCCCTCT CGGCTCACGT AGCTCCCACC AGATGGTTGA GGAGCTTATG 
ATCCTATTCA ATAGCTATGT AGCTGAATTC CTGGTGCACC AGGATAACAC AAAGCTGATC 
ACCCCATTGA GGTGCCAGGC TGAGCCCAAT CCTCAGCAAG TAGCTCAGTT AAAGGATAAA 
TACAGTCACT TGCTCCCTCT GTCCCTTCAT CTCTCTTATC GTCTCTGTGG CCAGACCACC 
AGCACTTTCC AAGGAACCAG TTCAGGATAC AACCTTTGCA TCCTGCGCCC GCTTTGGGAC 
CACCTGTGCC AAGCTGCTGA CAGCAAGGAC TATAACAAGA TGGTGGACTT AATTGCCACA 
GATGACATCC ACCCCACTCT GGCCCCAGCT GGACTGGAGT TCCGAAAGCT GCTCAGCCGG 
TCCTACTTTA GCCGCTCCAA CTCCACCAGC CTGTCCAGGG CTGGGCATTA CTCGTTGCAG 
GTGGACTGGT ATACTTGGGC ATCATCCCCC ATAAGGCGCT ATATTGATGT GGTGCTCCAG 
CGGCAGCTCC ATGCTGCCAT GACCAAGGGC CATCCTGGAT ATTCCCCTGA AGATATTGAC 
TTACTGTGCC ATGACTTCAA CCGGAAGAAT GGACGCGCAC AGGCTTACGA GCGCAAGGCC 
CACTGCCTTC ACCTTGCCAC CCAGCTCAAG ACCCAGCTGC TGACAAAGCT GTGCTTTGTG 
GTAAATGTGG AATCTGCAAA CCGCTTTTTC AAGGTGATTT TCCCCATGAA CCGGGATACC 
CTGCCTGATC CCCACCTTGT CAATTATCGT GCACTGCAAC TGGTAGAGCA GCCCACCTTC 
ATCCCTGAGC ACCAGAAAAT GAAGTTGGTT TGGAAGAGGC GAATATACTC TCTGGATAAG 
GGCAAAGCAT GGATGCCATC CTCTAAAACC CTGGTAGACA CCCATGTCAT GCCAGTAAGC 
AGTGTCATCT GGCAGGACCT GCTGGTTGCA ATCCAGAGTC AGAATTTTCA GAAAGCAGCA 
GCCTTGCTCC TAAGGTGTCG AGGGACTGTT CAGACCTCCC ACCATCGGTC CTTAGGCCAG 
GTGTCTCGAA GCAAGTGCTC GCACTATGTA GAGGTGATAG TAGAGCTAAG TACAGGGGAT 
GCCCTGCAGA TGCAACTGAC TACTGATGTC CAGAGGGGCT TCCTTGTGCC CTCCATTCAG 
ATGTGGATGT TGACCCCAGG TTTTGCCATT TGCCTGGAGC ATGCTGAGAA ACCCATTGAC 
TGCTTTTCCC GCTATGCTTC ACAGACAACG AAAGATGTGT ATTCTGAGGC ACAGGAATAT 
TCCCGGGTAT GGGAACCACT CTGTGCCATG GAGTCCGCCT CCAGTGCTGT GGCTGAGAAC 
GACTCTATTC TCTTGCATGG TGTTCCCATT ACTTGGGACC AAGAGCGGAA TGACCAGGGA 
CAGATGCAAG GTTCCTTCTG CTTAACCTAT AGCTTCCTCA AGGAATGTGC GATTGACATC 
AACTTTAATT TCTGCTACCT CTGCATCCGG TTGGAGAACC TGAAACTCCA GGGTCCAGTA 
GAAGGGGAGG AGGAGAGGGA AAAAGGAAAG GAGGCTGCTC TGTGTCAAGG CTTTAGGAAG 
CTCAGCTTGG GCCACACGGC AGCAAACCCA TCTGTGGTCA ATACTCTTGA CTCTTGTTTG 
AATGTAGACC CTGACACGTA TACCTGGGTG GCTCATGGGC TGACAGATGA AATGGATCAG 
GATTCCAAAA AAGTTGATCG AAGAGAAGAA CGACAGAGAA TCCATTTTTT CCTACATCAT 
GTAGCCATGG AGAGCATTCC CGTGGAGGTC ACCAGAAAGG AAGCTTGCTT CACCATTGAG 
ATGATCCCAA AGCTTCTGCC AGATATCCGG AAAGAGGAAG CCATCTGGAA ACTGAAATAT 
GCCTCACCAC TTGTCACCAG CATTGCCTTA GGCCAGCCCA TTAAGGAAGT GTCCTGCGTA 
GCCATGAAGT CCAGTATCCT GAAGCGCCAG ACCTTTGACA TTCCCCGAGG AAAATTCAAG 
CTCAATAAGA GTCAGAACAG TGCTGTGCTA GAAGCTCTTC AAAAGCCTTT TACCCTCATC 
CAAGGGCCAC CAGGCACTGG GAAGACGATG GTCGGGGTCC ATATTGTATA CTGGTTCCAT 
AAGCTAAACC AGGAGAAGCC TGAAACTGCT CCGCTGCCCT TGAGAGGGGA GAAGAAAGAC 
AAAGGGGAGA AGTGCATCCT ATATTGTGGG CCATCCAACA AATCTGTTGA TGTTGTTTCA 
GAAATCCTCC TTCAGTGGAA GGAGGAACTC AGACCATTGC AAGTATATAG TGAACAGATG 
GAGGCCATGG AATTCCCTGT ACCTGGGAGC TATAGAAATA TGTTTCGCAA AAATCTCCGG 
GAAGGGAAGA CAAAACCTGA GCTCAGACAG ATCATCCTTC ATCATCGTAT CCGGATGCCG 
TCCAATCCAT ATGCTCGCAA GATTATGGAA TTTGATACCA GAGTGAAGAT GGGGGATCAG 
TTTTCAGAGG AAGAGATTGA ACTATATAAG TCCATCACCA CGACGGCCCG GAAATATGAA 
CTAAGCAACC ATGACATAAT CCTCTGTACT TGCTCCTGTT CAGCCTCCTC CATTCTCACC 
AAACAACTCA ATGTCAAACA AATTCTCATT GATGAAGCTG GCATGTCCAC AGAGCCTGAA 
AGCCTCATCC CCCTTGTTAA CTTTCCCCGG GCTGAGAAGG TGGTGTTGCT AGGGGACCAC 
AAACAGCTTC AGCCTTTTGT AAAGAATGAT CACTGTCAAA ACCTGGGCTT GGAGAAGTCC 
CTCTTCGAGC GTTACCATCG TCAAGCCTGG ATGCTGGACA CACAGTATCG AATGCACAAG 
GACATCTGCA AATTCCCTTC CAAAGAATTT TATGACAATA AATTAAAGAC TAGCATGGAA 
CTAAGGCGAA CGCCAAGTGT CTTCAGCCAC CAAGATCAAG AAAGCTGTCC CATCATCTTT 
GGCTATGTCC AGGGAGAAGA GAAGAGTCTG GTAGTCTCCA CAGATGAAGG AAATGAAAAC 
TCCAAAGCTA ACCAAGATGA AGTGGAGCAG GTGATTCGAA TAGCAAAGCA GCTGACCTTG 
GATAGGACCA TCAAACCCAA GGACATTGCA ATCCTCAGCC CATACAATGC CCAGGTATCA 
GAGATCAACA AGAGACTATT AAAGGAGGGA ATTACTGGAG TGACTGTCTC CTCCATCAAG 
AAGAGCCAAG GAAGTGAGTG GCGATATGTC ATTCTAAGCA CAGTCCGCTC CTGCCCTCTT 
AGCGAAATTG ATGGGAAGCC CACCAAAAGC TGGCTGAAGA AACACCTGGG TTTTGTCAGT 
GACCCACATC AGGTCAATGT GGGGATCACT CGGGCCCAAG AGGGGCTCTG CATCATTGGA 
AATCACTACC TCTTGAGTTG CTGTCCAATG TGGCATCGGC TCATTGATTT CTACACCAAG 
AAGGGATGCT GTGTCATGGC TTCCTCAGTG CAAGTGCGTC AGAGGCCAGC TATCTACCAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023350215.1
CDS	326..7828
Translation	MDSSPTRTGFYMNINTATMKYRCFLCPSATQMSESIRDVTIHCDQPLKCQVEDKKSQCIWKFIIHSKDPLQHVALLKHQMGAIFSLVCPGLPQRQLYAQGSRFHVQDTAMTFQVEVLVECITFGSYEQWVAFDFGRRPVLVQKLYVQVGQKETLGPPPCIQDHNCLLELIRWHSGNRCVVPNVGRTAEQVNLLAKYKVSPQELENYRSKASDQVSITPVNYRERMHQFLYMEEEAQQELVAKLNLQVEVTLTSKMQTLAMGMKFAPSGELYAKVPIPCSLTPDTDQGYLLSRAVSTAYVAPVPAPDNHVYEVRVEVKATSEQNVWLLLPAHCCSALGLKDEDTQLMEIQFQIDKTIFGIWHQAIDALQDDRLVAPDVPACSLPSLRPPPQELRGNPKQKEAISFITGTTSGQSRVPPLLIYGPFGTGKTYTLAMATLEVIKQPNTKVLICTHTNSAADIYIREYFHAYVTAGHPEAVPLRVKNTESFLNQTDPTTLQYCCLSSDGRSFCFPTQEKLKQHRIIITTTTLSRSLCVPSGFFSHILIDEAAQMLECEAIIPLAYATQKTRIVLAGDHMQITPKLFSVGARKSADHTLLNRLFQYYQKETHKLASQSRVIFHENYRSTKAIISFVSRNFYVAKGNPIQASGQIPAHPCKYPLMFCHVAGTPERDITMTSWFNRAEIVEVIEKVQEFYRIWAYQWNSPDLKQICVVSHGSQVKILRQELRKKQLGEVAVENFENLPGREFRVIILSTVHTRDSLLNSSVPHLEFFSEARVLNTVMTRAQSQVVAVGDAVALCSFGRCSKVWKHFIQECIEMRSVSPVDLTMEQIKQDVAHRQRWARQNGLHPMNSPREHSLEEEDNDNDIPSWVSDAELNADDPILQELLDESKNVAVTVSEDGLLNVTSGSRSRYQGQQYLSFPVPVLQKYLRMQPKIYRKCELIKEAFEKASAFTIDDSPPLIIQIRGRTNCGMAFTGDQVLVEILTPELEEQGYSWRPMGRVVGILERGDRELIFICKMDEFDPRVMIPIDPSVTKIFVPELKDKPNTIPIRRLQRGHIKLISYEKITEETKRNQLFRVQIISWKERFYYPLGIILEILPLPLTLEQGLKILDMEYSLNANGRYPTPVIKEVNQHNCSKLNLHRGQWKDCRDYLTFTVDPQGSRDLDDAISVRDLGSHYEIAVHISDVASFMPKDGALDREARKRGATYYAPGKDPVAMLPSRLSQDLFSLLPQQERRTISLILTVEKESDRVVQWHITPSMICSDLQLTYEEAEVIIKSHPGGGAWLPLRLGTVEGCVVAAYHFSQVLRRSRLQGDCYYDQPDEDSPLGSRSSHQMVEELMILFNSYVAEFLVHQDNTKLITPLRCQAEPNPQQVAQLKDKYSHLLPLSLHLSYRLCGQTTSTFQGTSSGYNLCILRPLWDHLCQAADSKDYNKMVDLIATDDIHPTLAPAGLEFRKLLSRSYFSRSNSTSLSRAGHYSLQVDWYTWASSPIRRYIDVVLQRQLHAAMTKGHPGYSPEDIDLLCHDFNRKNGRAQAYERKAHCLHLATQLKTQLLTKLCFVVNVESANRFFKVIFPMNRDTLPDPHLVNYRALQLVEQPTFIPEHQKMKLVWKRRIYSLDKGKAWMPSSKTLVDTHVMPVSSVIWQDLLVAIQSQNFQKAAALLLRCRGTVQTSHHRSLGQVSRSKCSHYVEVIVELSTGDALQMQLTTDVQRGFLVPSIQMWMLTPGFAICLEHAEKPIDCFSRYASQTTKDVYSEAQEYSRVWEPLCAMESASSAVAENDSILLHGVPITWDQERNDQGQMQGSFCLTYSFLKECAIDINFNFCYLCIRLENLKLQGPVEGEEEREKGKEAALCQGFRKLSLGHTAANPSVVNTLDSCLNVDPDTYTWVAHGLTDEMDQDSKKVDRREERQRIHFFLHHVAMESIPVEVTRKEACFTIEMIPKLLPDIRKEEAIWKLKYASPLVTSIALGQPIKEVSCVAMKSSILKRQTFDIPRGKFKLNKSQNSAVLEALQKPFTLIQGPPGTGKTMVGVHIVYWFHKLNQEKPETAPLPLRGEKKDKGEKCILYCGPSNKSVDVVSEILLQWKEELRPLQVYSEQMEAMEFPVPGSYRNMFRKNLREGKTKPELRQIILHHRIRMPSNPYARKIMEFDTRVKMGDQFSEEEIELYKSITTTARKYELSNHDIILCTCSCSASSILTKQLNVKQILIDEAGMSTEPESLIPLVNFPRAEKVVLLGDHKQLQPFVKNDHCQNLGLEKSLFERYHRQAWMLDTQYRMHKDICKFPSKEFYDNKLKTSMELRRTPSVFSHQDQESCPIIFGYVQGEEKSLVVSTDEGNENSKANQDEVEQVIRIAKQLTLDRTIKPKDIAILSPYNAQVSEINKRLLKEGITGVTVSSIKKSQGSEWRYVILSTVRSCPLSEIDGKPTKSWLKKHLGFVSDPHQVNVGITRAQEGLCIIGNHYLLSCCPMWHRLIDFYTKKGCCVMASSVQVRQRPAIYH

Target ORF information:

RefSeq Version	XM_023494447.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii helicase with zinc finger 2 (HELZ2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023494447.1

ORF Insert Sequence:

ATGGACTCCT CTCCTACCAG GACCGGCTTT TACATGAATA TCAACACTGC CACAATGAAA 
TACAGGTGCT TCCTGTGCCC ATCTGCCACT CAGATGTCCG AGTCTATACG TGATGTAACT 
ATTCACTGTG ACCAGCCCTT AAAATGCCAG GTAGAGGACA AGAAATCGCA ATGCATATGG 
AAATTTATCA TCCATTCTAA GGACCCACTG CAGCACGTGG CCCTCCTCAA ACATCAGATG 
GGAGCCATCT TTTCCTTGGT GTGTCCTGGG CTTCCACAAA GACAGCTTTA TGCCCAAGGC 
TCAAGGTTCC ATGTGCAGGA TACAGCGATG ACCTTCCAGG TGGAGGTGCT GGTAGAGTGT 
ATCACCTTTG GCAGCTATGA ACAATGGGTA GCTTTTGACT TTGGACGCCG ACCTGTCCTG 
GTACAGAAGC TATATGTTCA AGTGGGCCAA AAGGAGACCC TTGGGCCTCC CCCCTGTATC 
CAGGACCATA ATTGTCTTCT TGAGTTGATC CGCTGGCACA GTGGAAATCG ATGTGTGGTG 
CCCAATGTGG GCAGGACAGC TGAGCAGGTG AATCTGCTAG CTAAGTACAA AGTGAGCCCC 
CAGGAACTGG AGAACTACAG GTCAAAGGCT TCAGACCAGG TCTCTATAAC TCCAGTCAAC 
TATCGGGAGA GGATGCACCA ATTCTTGTAT ATGGAAGAGG AGGCTCAACA GGAGCTGGTG 
GCCAAATTGA ACCTTCAAGT CGAAGTCACA TTGACCTCCA AGATGCAGAC CTTGGCAATG 
GGCATGAAGT TTGCACCATC TGGGGAATTG TATGCTAAGG TTCCAATTCC TTGTTCCCTG 
ACACCAGACA CAGACCAGGG CTATTTGCTA AGTAGAGCTG TTAGCACTGC TTACGTGGCA 
CCTGTCCCTG CCCCTGACAA TCATGTGTAT GAGGTTCGGG TGGAAGTTAA AGCCACTTCA 
GAGCAGAATG TGTGGCTCCT ACTGCCAGCC CACTGCTGCT CTGCCTTGGG ACTTAAGGAT 
GAAGACACAC AGCTTATGGA AATCCAGTTT CAAATCGACA AGACTATATT TGGGATATGG 
CACCAAGCCA TTGATGCACT TCAGGATGAT AGACTGGTGG CACCTGATGT ACCTGCCTGT 
TCTCTGCCTT CTTTGAGGCC CCCTCCCCAG GAACTCCGTG GTAATCCCAA GCAGAAGGAG 
GCCATCTCCT TTATCACAGG GACAACGAGT GGTCAGAGCC GAGTGCCTCC ACTGCTCATT 
TATGGCCCCT TTGGGACAGG CAAGACATAC ACACTAGCTA TGGCCACGCT AGAGGTTATC 
AAACAGCCAA ACACAAAAGT GTTGATCTGT ACTCACACCA ACAGTGCTGC TGACATCTAC 
ATCCGTGAGT ACTTTCATGC TTATGTCACA GCTGGACACC CTGAGGCTGT GCCATTGAGG 
GTCAAAAACA CTGAAAGCTT CCTCAACCAG ACAGACCCGA CCACTCTGCA GTATTGCTGC 
CTCTCAAGTG ATGGACGGTC CTTTTGCTTC CCAACTCAGG AGAAACTAAA GCAGCACCGC 
ATTATAATCA CTACCACTAC CTTGTCCCGC TCCCTATGTG TGCCATCTGG GTTTTTTTCC 
CACATTCTCA TTGATGAGGC TGCTCAGATG TTAGAATGTG AAGCCATCAT CCCACTGGCC 
TATGCTACCC AGAAGACACG CATTGTCCTT GCCGGGGACC ACATGCAAAT CACCCCCAAA 
CTCTTCAGTG TTGGGGCCAG GAAGTCTGCT GACCATACAC TGCTTAATCG GCTGTTCCAG 
TACTATCAGA AGGAGACTCA TAAATTGGCT TCCCAGAGCA GGGTCATCTT CCATGAAAAC 
TACCGTTCCA CCAAAGCCAT CATTTCCTTT GTGTCTCGGA ATTTCTATGT GGCTAAGGGT 
AACCCTATTC AGGCTAGCGG ACAGATCCCT GCACACCCTT GCAAGTACCC ACTCATGTTT 
TGCCATGTGG CTGGTACTCC TGAGAGAGAC ATAACAATGA CATCATGGTT TAACAGGGCT 
GAGATAGTGG AGGTGATTGA GAAGGTGCAA GAATTCTATC GTATCTGGGC CTACCAATGG 
AATAGCCCTG ACCTAAAGCA AATTTGTGTT GTGTCCCATG GTTCTCAGGT AAAAATATTG 
AGGCAGGAAC TGAGGAAGAA ACAACTAGGA GAAGTAGCAG TGGAGAATTT TGAAAATCTG 
CCAGGTCGTG AATTCCGAGT GATCATCCTC AGCACAGTCC ACACTCGGGA CAGCCTGCTC 
AACAGCTCAG TCCCCCACCT AGAATTCTTC AGTGAGGCTC GGGTGCTGAA CACCGTGATG 
ACCAGAGCCC AGTCCCAGGT GGTTGCTGTG GGCGATGCTG TGGCCCTTTG TTCTTTTGGA 
CGTTGTAGCA AGGTATGGAA GCACTTCATC CAAGAGTGTA TAGAGATGCG CAGTGTGTCC 
CCTGTGGACC TCACAATGGA ACAAATCAAG CAAGATGTGG CACACAGGCA GCGCTGGGCC 
CGGCAAAATG GACTGCATCC TATGAACTCT CCCAGGGAGC ACTCGTTAGA AGAGGAAGAC 
AATGACAATG ACATTCCCTC ATGGGTGTCT GATGCTGAGC TCAATGCTGA TGATCCCATC 
CTCCAGGAGT TGCTGGATGA GAGTAAGAAT GTGGCAGTGA CAGTAAGTGA GGATGGGCTG 
CTGAATGTGA CATCAGGGTC CCGGTCCCGC TATCAGGGAC AACAGTATTT AAGCTTTCCT 
GTGCCAGTGT TGCAGAAATA TCTCCGAATG CAACCCAAGA TATATAGGAA ATGTGAACTG 
ATAAAGGAGG CCTTTGAGAA GGCATCTGCC TTCACCATAG ATGACTCCCC ACCCCTCATT 
ATTCAGATCA GAGGACGCAC CAACTGTGGA ATGGCCTTCA CTGGGGATCA AGTTCTAGTG 
GAAATCCTGA CTCCAGAATT GGAGGAACAG GGGTACTCCT GGCGCCCCAT GGGCCGAGTG 
GTAGGAATAC TAGAAAGAGG TGACCGTGAA CTCATCTTCA TTTGCAAAAT GGATGAGTTT 
GACCCCCGAG TTATGATCCC CATTGACCCG TCAGTCACCA AGATCTTTGT GCCAGAGTTA 
AAGGATAAGC CCAACACGAT TCCTATCCGC CGCCTACAAC GAGGACACAT CAAGCTTATT 
AGCTATGAGA AGATAACAGA GGAGACCAAG CGTAATCAGC TCTTTCGTGT TCAGATCATC 
TCTTGGAAAG AGAGGTTCTA TTATCCCTTA GGCATCATTC TTGAGATTCT TCCCTTGCCT 
TTGACCTTAG AACAAGGTTT AAAGATTCTG GACATGGAAT ATTCTCTGAA TGCTAATGGA 
AGATACCCAA CTCCAGTAAT TAAGGAAGTG AACCAACACA ACTGTAGTAA ATTGAATCTG 
CACCGGGGAC AGTGGAAGGA CTGCCGGGAT TATTTGACCT TTACTGTGGA CCCGCAGGGC 
TCCCGGGATC TGGATGATGC TATCAGTGTC CGGGACCTGG GAAGCCATTA TGAAATTGCT 
GTCCATATAT CTGATGTGGC TAGCTTCATG CCAAAGGATG GTGCACTTGA CCGAGAGGCT 
AGAAAGCGAG GTGCTACCTA CTATGCTCCT GGAAAGGATC CAGTGGCCAT GCTCCCTTCC 
AGGCTCAGCC AGGACCTGTT CAGTCTCCTT CCCCAGCAAG AACGTCGCAC TATCTCCCTG 
ATTCTTACAG TGGAGAAGGA AAGTGACCGA GTAGTGCAAT GGCATATCAC ACCATCTATG 
ATATGCTCTG ACCTCCAACT GACGTATGAG GAGGCTGAGG TTATTATTAA GAGTCATCCT 
GGGGGTGGGG CCTGGCTGCC CCTCCGCCTG GGCACTGTAG AAGGCTGTGT TGTGGCTGCT 
TATCACTTCT CCCAAGTCCT CCGCAGAAGC CGCCTTCAGG GAGATTGCTA CTATGACCAA 
CCAGATGAAG ACAGCCCTCT CGGCTCACGT AGCTCCCACC AGATGGTTGA GGAGCTTATG 
ATCCTATTCA ATAGCTATGT AGCTGAATTC CTGGTGCACC AGGATAACAC AAAGCTGATC 
ACCCCATTGA GGTGCCAGGC TGAGCCCAAT CCTCAGCAAG TAGCTCAGTT AAAGGATAAA 
TACAGTCACT TGCTCCCTCT GTCCCTTCAT CTCTCTTATC GTCTCTGTGG CCAGACCACC 
AGCACTTTCC AAGGAACCAG TTCAGGATAC AACCTTTGCA TCCTGCGCCC GCTTTGGGAC 
CACCTGTGCC AAGCTGCTGA CAGCAAGGAC TATAACAAGA TGGTGGACTT AATTGCCACA 
GATGACATCC ACCCCACTCT GGCCCCAGCT GGACTGGAGT TCCGAAAGCT GCTCAGCCGG 
TCCTACTTTA GCCGCTCCAA CTCCACCAGC CTGTCCAGGG CTGGGCATTA CTCGTTGCAG 
GTGGACTGGT ATACTTGGGC ATCATCCCCC ATAAGGCGCT ATATTGATGT GGTGCTCCAG 
CGGCAGCTCC ATGCTGCCAT GACCAAGGGC CATCCTGGAT ATTCCCCTGA AGATATTGAC 
TTACTGTGCC ATGACTTCAA CCGGAAGAAT GGACGCGCAC AGGCTTACGA GCGCAAGGCC 
CACTGCCTTC ACCTTGCCAC CCAGCTCAAG ACCCAGCTGC TGACAAAGCT GTGCTTTGTG 
GTAAATGTGG AATCTGCAAA CCGCTTTTTC AAGGTGATTT TCCCCATGAA CCGGGATACC 
CTGCCTGATC CCCACCTTGT CAATTATCGT GCACTGCAAC TGGTAGAGCA GCCCACCTTC 
ATCCCTGAGC ACCAGAAAAT GAAGTTGGTT TGGAAGAGGC GAATATACTC TCTGGATAAG 
GGCAAAGCAT GGATGCCATC CTCTAAAACC CTGGTAGACA CCCATGTCAT GCCAGTAAGC 
AGTGTCATCT GGCAGGACCT GCTGGTTGCA ATCCAGAGTC AGAATTTTCA GAAAGCAGCA 
GCCTTGCTCC TAAGGTGTCG AGGGACTGTT CAGACCTCCC ACCATCGGTC CTTAGGCCAG 
GTGTCTCGAA GCAAGTGCTC GCACTATGTA GAGGTGATAG TAGAGCTAAG TACAGGGGAT 
GCCCTGCAGA TGCAACTGAC TACTGATGTC CAGAGGGGCT TCCTTGTGCC CTCCATTCAG 
ATGTGGATGT TGACCCCAGG TTTTGCCATT TGCCTGGAGC ATGCTGAGAA ACCCATTGAC 
TGCTTTTCCC GCTATGCTTC ACAGACAACG AAAGATGTGT ATTCTGAGGC ACAGGAATAT 
TCCCGGGTAT GGGAACCACT CTGTGCCATG GAGTCCGCCT CCAGTGCTGT GGCTGAGAAC 
GACTCTATTC TCTTGCATGG TGTTCCCATT ACTTGGGACC AAGAGCGGAA TGACCAGGGA 
CAGATGCAAG GTTCCTTCTG CTTAACCTAT AGCTTCCTCA AGGAATGTGC GATTGACATC 
AACTTTAATT TCTGCTACCT CTGCATCCGG TTGGAGAACC TGAAACTCCA GGGTCCAGTA 
GAAGGGGAGG AGGAGAGGGA AAAAGGAAAG GAGGCTGCTC TGTGTCAAGG CTTTAGGAAG 
CTCAGCTTGG GCCACACGGC AGCAAACCCA TCTGTGGTCA ATACTCTTGA CTCTTGTTTG 
AATGTAGACC CTGACACGTA TACCTGGGTG GCTCATGGGC TGACAGATGA AATGGATCAG 
GATTCCAAAA AAGTTGATCG AAGAGAAGAA CGACAGAGAA TCCATTTTTT CCTACATCAT 
GTAGCCATGG AGAGCATTCC CGTGGAGGTC ACCAGAAAGG AAGCTTGCTT CACCATTGAG 
ATGATCCCAA AGCTTCTGCC AGATATCCGG AAAGAGGAAG CCATCTGGAA ACTGAAATAT 
GCCTCACCAC TTGTCACCAG CATTGCCTTA GGCCAGCCCA TTAAGGAAGT GTCCTGCGTA 
GCCATGAAGT CCAGTATCCT GAAGCGCCAG ACCTTTGACA TTCCCCGAGG AAAATTCAAG 
CTCAATAAGA GTCAGAACAG TGCTGTGCTA GAAGCTCTTC AAAAGCCTTT TACCCTCATC 
CAAGGGCCAC CAGGCACTGG GAAGACGATG GTCGGGGTCC ATATTGTATA CTGGTTCCAT 
AAGCTAAACC AGGAGAAGCC TGAAACTGCT CCGCTGCCCT TGAGAGGGGA GAAGAAAGAC 
AAAGGGGAGA AGTGCATCCT ATATTGTGGG CCATCCAACA AATCTGTTGA TGTTGTTTCA 
GAAATCCTCC TTCAGTGGAA GGAGGAACTC AGACCATTGC AAGTATATAG TGAACAGATG 
GAGGCCATGG AATTCCCTGT ACCTGGGAGC TATAGAAATA TGTTTCGCAA AAATCTCCGG 
GAAGGGAAGA CAAAACCTGA GCTCAGACAG ATCATCCTTC ATCATCGTAT CCGGATGCCG 
TCCAATCCAT ATGCTCGCAA GATTATGGAA TTTGATACCA GAGTGAAGAT GGGGGATCAG 
TTTTCAGAGG AAGAGATTGA ACTATATAAG TCCATCACCA CGACGGCCCG GAAATATGAA 
CTAAGCAACC ATGACATAAT CCTCTGTACT TGCTCCTGTT CAGCCTCCTC CATTCTCACC 
AAACAACTCA ATGTCAAACA AATTCTCATT GATGAAGCTG GCATGTCCAC AGAGCCTGAA 
AGCCTCATCC CCCTTGTTAA CTTTCCCCGG GCTGAGAAGG TGGTGTTGCT AGGGGACCAC 
AAACAGCTTC AGCCTTTTGT AAAGAATGAT CACTGTCAAA ACCTGGGCTT GGAGAAGTCC 
CTCTTCGAGC GTTACCATCG TCAAGCCTGG ATGCTGGACA CACAGTATCG AATGCACAAG 
GACATCTGCA AATTCCCTTC CAAAGAATTT TATGACAATA AATTAAAGAC TAGCATGGAA 
CTAAGGCGAA CGCCAAGTGT CTTCAGCCAC CAAGATCAAG AAAGCTGTCC CATCATCTTT 
GGCTATGTCC AGGGAGAAGA GAAGAGTCTG GTAGTCTCCA CAGATGAAGG AAATGAAAAC 
TCCAAAGCTA ACCAAGATGA AGTGGAGCAG GTGATTCGAA TAGCAAAGCA GCTGACCTTG 
GATAGGACCA TCAAACCCAA GGACATTGCA ATCCTCAGCC CATACAATGC CCAGGTATCA 
GAGATCAACA AGAGACTATT AAAGGAGGGA ATTACTGGAG TGACTGTCTC CTCCATCAAG 
AAGAGCCAAG GAAGTGAGTG GCGATATGTC ATTCTAAGCA CAGTCCGCTC CTGCCCTCTT 
AGCGAAATTG ATGGGAAGCC CACCAAAAGC TGGCTGAAGA AACACCTGGG TTTTGTCAGT 
GACCCACATC AGGTCAATGT GGGGATCACT CGGGCCCAAG AGGGGCTCTG CATCATTGGA 
AATCACTACC TCTTGAGTTG CTGTCCAATG TGGCATCGGC TCATTGATTT CTACACCAAG 
AAGGGATGCT GTGTCATGGC TTCCTCAGTG CAAGTGCGTC AGAGGCCAGC TATCTACCAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HELZ2 ( XM_012554098.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012409552.1 Sarcophilus harrisii helicase with zinc finger 2 (HELZ2), transcript variant X1, mRNA.

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CloneID	OSa119203
Clone ID Related Accession (Same CDS sequence)	XM_012554098.2 , XM_012554098.3 , XM_012554098.1
Accession Version	XM_012554098.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	helicase with zinc finger domain 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816669.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012554098.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCAC TCAAAAAGGG GCTTCTGGCT CGGCTCCAGA GCCAACTGGA TCAGCTTCAG 
AGCCAACTGG ACCTACGTGT GGGCTGCTCA GTCTGCTCAG AGCCATTGAA TGAGAGCACA 
TATAAATGGC ATACCATAGA ACACAACTGC CCCCGTGAGA TTCTCCTGGC AAGGGCCAAG 
GGAGCTCCTG AGCATAAAGA GTGGAGAAAG GTTGGACGCA GGCCCAGCTT CCCCCGGCCC 
AGACGTTACG AAGTATGCCG CTACTACAAA CCTGGGATGG GCTGCAGGAA ACACCGCAAC 
CAGTGCACAT TTGCCCGAAG CTTAGAGGAA GCATTGGTTT GGACTTTTGA GAGGGAAAAC 
AATATCCCCA GACTGTGGCT GAAAGCTAAA GTACAGGGAA GCTTGTATCA AGGGACTCCC 
AGGATTCTGA CTGCAGCTGA AGAAATAGGG GCTGAATTTG GAGGCTACTT TCAGGAACTT 
TGTAAGCCTT GCTTCCGGAA CTGCCCTGCA CAGGTGACTC TCCGGGGCCC AAACCTGCCA 
TGCCCACAGC ATGGGCCCTC TCCCCTGCTT ATCGTACACA TAAGTGAGGG TTTTAGAAAA 
AAGCAGCTAG TGGAGATCCG GCCCCGGCCC CGGCAAGGCC ACCTACTGGA ATATTGTATG 
TACGTGTCTC GAGGACATCC GTGTCGGCAC GGAGCTGTCC GCTGCCAATA TGCTCACAGT 
GATGTGGAGA TGGCTGTGTG GGAAGCAGAA TCAATGGGCT GCCTTACTCG CTCTGACTTG 
CTTGTGCTAC CAGCCTCACT GGAATCTGGA GCAGGCTCTG GGACCAGCGG TGAGGTTGGT 
GGTAGAAATG GGAGCCCATC TGCTCCACCC CAGATTCAGC TGTATTGCCG TGTCTGTCTT 
GTAACTTGCA GCTCCCAAGA GAGTTTTGAG AACCATTGCT CATCCCTGGA ACACACACAG 
ATGTTGTCCT ATGACCATGG GGCCCTATGG GAATACCGGG CCCCCCCCAT TGGGCTCCGC 
AAATTCAAAC TTTGCAGCAG GCTAGATATT TGTGAATATG GGAATGGCTG CACTCAGGCA 
CACTCCCAGG AAGAGCTTCA AGAGTGGATT ATGCGGGCTC AGGCAGCCCA GATGAGAGAA 
GAATCGGCCC GGCAGGATGG ACTCCTCTCC TACCAGGACC GGCTTTTACA TGAATATCAA 
CACTGCCACA ATGAAATACA GGTGATGTCC GAGTCTATAC GTGATGTAAC TATTCACTGT 
GACCAGCCCT TAAAATGCCA GGTAGAGGAC AAGAAATCGC AATGCATATG GAAATTTATC 
ATCCATTCTA AGGACCCACT GCAGCACGTG GCCCTCCTCA AACATCAGAT GGGAGCCATC 
TTTTCCTTGG TGTGTCCTGG GCTTCCACAA AGACAGCTTT ATGCCCAAGG CTCAAGGTTC 
CATGTGCAGG ATACAGCGAT GACCTTCCAG GTGGAGGTGC TGGTAGAGTG TATCACCTTT 
GGCAGCTATG AACAATGGGT AGCTTTTGAC TTTGGACGCC GACCTGTCCT GGTACAGAAG 
CTATATGTTC AAGTGGGCCA AAAGGAGACC CTTGGGCCTC CCCCCTGTAT CCAGGACCAT 
AATTGTCTTC TTGAGTTGAT CCGCTGGCAC AGTGGAAATC GATGTGTGGT GCCCAATGTG 
GGCAGGACAG CTGAGCAGGT GAATCTGCTA GCTAAGTACA AAGTGAGCCC CCAGGAACTG 
GAGAACTACA GGTCAAAGGC TTCAGACCAG GTCTCTATAA CTCCAGTCAA CTATCGGGAG 
AGGATGCACC AATTCTTGTA TATGGAAGAG GAGGCTCAAC AGGAGCTGGT GGCCAAATTG 
AACCTTCAAG TCGAAGTCAC ATTGACCTCC AAGATGCAGA CCTTGGCAAT GGGCATGAAG 
TTTGCACCAT CTGGGGAATT GTATGCTAAG GTTCCAATTC CTTGTTCCCT GACACCAGAC 
ACAGACCAGG GCTATTTGCT AAGTAGAGCT GTTAGCACTG CTTACGTGGC ACCTGTCCCT 
GCCCCTGACA ATCATGTGTA TGAGGTTCGG GTGGAAGTTA AAGCCACTTC AGAGCAGAAT 
GTGTGGCTCC TACTGCCAGC CCACTGCTGC TCTGCCTTGG GACTTAAGGA TGAAGACACA 
CAGCTTATGG AAATCCAGTT TCAAATCGAC AAGACTATAT TTGGGATATG GCACCAAGCC 
ATTGATGCAC TTCAGGATGA TAGACTGGTG GCACCTGATG TACCTGCCTG TTCTCTGCCT 
TCTTTGAGGC CCCCTCCCCA GGAACTCCGT GGTAATCCCA AGCAGAAGGA GGCCATCTCC 
TTTATCACAG GGACAACGAG TGGTCAGAGC CGAGTGCCTC CACTGCTCAT TTATGGCCCC 
TTTGGGACAG GCAAGACATA CACACTAGCT ATGGCCACGC TAGAGGTTAT CAAACAGCCA 
AACACAAAAG TGTTGATCTG TACTCACACC AACAGTGCTG CTGACATCTA CATCCGTGAG 
TACTTTCATG CTTATGTCAC AGCTGGACAC CCTGAGGCTG TGCCATTGAG GGTCAAAAAC 
ACTGAAAGCT TCCTCAACCA GACAGACCCG ACCACTCTGC AGTATTGCTG CCTCTCAAGT 
GATGGACGGT CCTTTTGCTT CCCAACTCAG GAGAAACTAA AGCAGCACCG CATTATAATC 
ACTACCACTA CCTTGTCCCG CTCCCTATGT GTGCCATCTG GGTTTTTTTC CCACATTCTC 
ATTGATGAGG CTGCTCAGAT GTTAGAATGT GAAGCCATCA TCCCACTGGC CTATGCTACC 
CAGAAGACAC GCATTGTCCT TGCCGGGGAC CACATGCAAA TCACCCCCAA ACTCTTCAGT 
GTTGGGGCCA GGAAGTCTGC TGACCATACA CTGCTTAATC GGCTGTTCCA GTACTATCAG 
AAGGAGACTC ATAAATTGGC TTCCCAGAGC AGGGTCATCT TCCATGAAAA CTACCGTTCC 
ACCAAAGCCA TCATTTCCTT TGTGTCTCGG AATTTCTATG TGGCTAAGGG TAACCCTATT 
CAGGCTAGCG GACAGATCCC TGCACACCCT TGCAAGTACC CACTCATGTT TTGCCATGTG 
GCTGGTACTC CTGAGAGAGA CATAACAATG ACATCATGGT TTAACAGGGC TGAGATAGTG 
GAGGTGATTG AGAAGGTGCA AGAATTCTAT CGTATCTGGG CCTACCAATG GAATAGCCCT 
GACCTAAAGC AAATTTGTGT TGTGTCCCAT GGTTCTCAGG TAAAAATATT GAGGCAGGAA 
CTGAGGAAGA AACAACTAGG AGAAGTAGCA GTGGAGAATT TTGAAAATCT GCCAGGTCGT 
GAATTCCGAG TGATCATCCT CAGCACAGTC CACACTCGGG ACAGCCTGCT CAACAGCTCA 
GTCCCCCACC TAGAATTCTT CAGTGAGGCT CGGGTGCTGA ACACCGTGAT GACCAGAGCC 
CAGTCCCAGG TGGTTGCTGT GGGCGATGCT GTGGCCCTTT GTTCTTTTGG ACGTTGTAGC 
AAGGTATGGA AGCACTTCAT CCAAGAGTGT ATAGAGATGC GCAGTGTGTC CCCTGTGGAC 
CTCACAATGG AACAAATCAA GCAAGATGTG GCACACAGGC AGCGCTGGGC CCGGCAAAAT 
GGACTGCATC CTATGAACTC TCCCAGGGAG CACTCGTTAG AAGAGGAAGA CAATGACAAT 
GACATTCCCT CATGGGTGTC TGATGCTGAG CTCAATGCTG ATGATCCCAT CCTCCAGGAG 
TTGCTGGATG AGAGTAAGAA TGTGGCAGTG ACAGTAAGTG AGGATGGGCT GCTGAATGTG 
ACATCAGGGT CCCGGTCCCG CTATCAGGGA CAACAGTATT TAAGCTTTCC TGTGCCAGTG 
TTGCAGAAAT ATCTCCGAAT GCAACCCAAG ATATATAGGA AATGTGAACT GATAAAGGAG 
GCCTTTGAGA AGGCATCTGC CTTCACCATA GATGACTCCC CACCCCTCAT TATTCAGATC 
AGAGGACGCA CCAACTGTGG AATGGCCTTC ACTGGGGATC AAGTTCTAGT GGAAATCCTG 
ACTCCAGAAT TGGAGGAACA GGGGTACTCC TGGCGCCCCA TGGGCCGAGT GGTAGGAATA 
CTAGAAAGAG GTGACCGTGA ACTCATCTTC ATTTGCAAAA TGGATGAGTT TGACCCCCGA 
GTTATGATCC CCATTGACCC GTCAGTCACC AAGATCTTTG TGCCAGAGTT AAAGGATAAG 
CCCAACACGA TTCCTATCCG CCGCCTACAA CGAGGACACA TCAAGCTTAT TAGCTATGAG 
AAGATAACAG AGGAGACCAA GCGTAATCAG CTCTTTCGTG TTCAGATCAT CTCTTGGAAA 
GAGAGGTTCT ATTATCCCTT AGGCATCATT CTTGAGATTC TTCCCTTGCC TTTGACCTTA 
GAACAAGGTT TAAAGATTCT GGACATGGAA TATTCTCTGA ATGCTAATGG AAGATACCCA 
ACTCCAGTAA TTAAGGAAGT GAACCAACAC AACTGTAGTA AATTGAATCT GCACCGGGGA 
CAGTGGAAGG ACTGCCGGGA TTATTTGACC TTTACTGTGG ACCCGCAGGG CTCCCGGGAT 
CTGGATGATG CTATCAGTGT CCGGGACCTG GGAAGCCATT ATGAAATTGC TGTCCATATA 
TCTGATGTGG CTAGCTTCAT GCCAAAGGAT GGTGCACTTG ACCGAGAGGC TAGAAAGCGA 
GGTGCTACCT ACTATGCTCC TGGAAAGGAT CCAGTGGCCA TGCTCCCTTC CAGGCTCAGC 
CAGGACCTGT TCAGTCTCCT TCCCCAGCAA GAACGTCGCA CTATCTCCCT GATTCTTACA 
GTGGAGAAGG AAAGTGACCG AGTAGTGCAA TGGCATATCA CACCATCTAT GATATGCTCT 
GACCTCCAAC TGACGTATGA GGAGGCTGAG GTTATTATTA AGAGTCATCC TGGGGGTGGG 
GCCTGGCTGC CCCTCCGCCT GGGCACTGTA GAAGGCTGTG TTGTGGCTGC TTATCACTTC 
TCCCAAGTCC TCCGCAGAAG CCGCCTTCAG GGAGATTGCT ACTATGACCA ACCAGATGAA 
GACAGCCCTC TCGGCTCACG TAGCTCCCAC CAGATGGTTG AGGAGCTTAT GATCCTATTC 
AATAGCTATG TAGCTGAATT CCTGGTGCAC CAGGATAACA CAAAGCTGAT CACCCCATTG 
AGGTGCCAGG CTGAGCCCAA TCCTCAGCAA GTAGCTCAGT TAAAGGATAA ATACAGTCAC 
TTGCTCCCTC TGTCCCTTCA TCTCTCTTAT CGTCTCTGTG GCCAGACCAC CAGCACTTTC 
CAAGGAACCA GTTCAGGATA CAACCTTTGC ATCCTGCGCC CGCTTTGGGA CCACCTGTGC 
CAAGCTGCTG ACAGCAAGGA CTATAACAAG ATGGTGGACT TAATTGCCAC AGATGACATC 
CACCCCACTC TGGCCCCAGC TGGACTGGAG TTCCGAAAGC TGCTCAGCCG GTCCTACTTT 
AGCCGCTCCA ACTCCACCAG CCTGTCCAGG GCTGGGCATT ACTCGTTGCA GGTGGACTGG 
TATACTTGGG CATCATCCCC CATAAGGCGC TATATTGATG TGGTGCTCCA GCGGCAGCTC 
CATGCTGCCA TGACCAAGGG CCATCCTGGA TATTCCCCTG AAGATATTGA CTTACTGTGC 
CATGACTTCA ACCGGAAGAA TGGACGCGCA CAGGCTTACG AGCGCAAGGC CCACTGCCTT 
CACCTTGCCA CCCAGCTCAA GACCCAGCTG CTGACAAAGC TGTGCTTTGT GGTAAATGTG 
GAATCTGCAA ACCGCTTTTT CAAGGTGATT TTCCCCATGA ACCGGGATAC CCTGCCTGAT 
CCCCACCTTG TCAATTATCG TGCACTGCAA CTGGTAGAGC AGCCCACCTT CATCCCTGAG 
CACCAGAAAA TGAAGTTGGT TTGGAAGAGG CGAATATACT CTCTGGATAA GGGCAAAGCA 
TGGATGCCAT CCTCTAAAAC CCTGGTAGAC ACCCATGTCA TGCCAGTAAG CAGTGTCATC 
TGGCAGGACC TGCTGGTTGC AATCCAGAGT CAGAATTTTC AGAAAGCAGC AGCCTTGCTC 
CTAAGGTGTC GAGGGACTGT TCAGACCTCC CACCATCGGT CCTTAGGCCA GGTGTCTCGA 
AGCAAGTGCT CGCACTATGT AGAGGTGATA GTAGAGCTAA GTACAGGGGA TGCCCTGCAG 
ATGCAACTGA CTACTGATGT CCAGAGGGGC TTCCTTGTGC CCTCCATTCA GATGTGGATG 
TTGACCCCAG GTTTTGCCAT TTGCCTGGAG CATGCTGAGA AACCCATTGA CTGCTTTTCC 
CGCTATGCTT CACAGACAAC GAAAGATGTG TATTCTGAGG CACAGGAATA TTCCCGGGTA 
TGGGAACCAC TCTGTGCCAT GGAGTCCGCC TCCAGTGCTG TGGCTGAGAA CGACTCTATT 
CTCTTGCATG GTGTTCCCAT TACTTGGGAC CAAGAGCGGA ATGACCAGGG ACAGATGCAA 
GGTTCCTTCT GCTTAACCTA TAGCTTCCTC AAGGAATGTG CGATTGACAT CAACTTTAAT 
TTCTGCTACC TCTGCATCCG GTTGGAGAAC CTGAAACTCC AGGGTCCAGT AGAAGGGGAG 
GAGGAGAGGG AAAAAGGAAA GGAGGCTGCT CTGTGTCAAG GCTTTAGGAA GCTCAGCTTG 
GGCCACACGG CAGCAAACCC ATCTGTGGTC AATACTCTTG ACTCTTGTTT GAATGTAGAC 
CCTGACACGT ATACCTGGGT GGCTCATGGG CTGACAGATG AAATGGATCA GGATTCCAAA 
AAAGTTGATC GAAGAGAAGA ACGACAGAGA ATCCATTTTT TCCTACATCA TGTAGCCATG 
GAGAGCATTC CCGTGGAGGT CACCAGAAAG GAAGCTTGCT TCACCATTGA GATGATCCCA 
AAGCTTCTGC CAGATATCCG GAAAGAGGAA GCCATCTGGA AACTGAAATA TGCCTCACCA 
CTTGTCACCA GCATTGCCTT AGGCCAGCCC ATTAAGGAAG TGTCCTGCGT AGCCATGAAG 
TCCAGTATCC TGAAGCGCCA GACCTTTGAC ATTCCCCGAG GAAAATTCAA GCTCAATAAG 
AGTCAGAACA GTGCTGTGCT AGAAGCTCTT CAAAAGCCTT TTACCCTCAT CCAAGGGCCA 
CCAGGCACTG GGAAGACGAT GGTCGGGGTC CATATTGTAT ACTGGTTCCA TAAGCTAAAC 
CAGGAGAAGC CTGAAACTGC TCCGCTGCCC TTGAGAGGGG AGAAGAAAGA CAAAGGGGAG 
AAGTGCATCC TATATTGTGG GCCATCCAAC AAATCTGTTG ATGTTGTTTC AGAAATCCTC 
CTTCAGTGGA AGGAGGAACT CAGACCATTG CAAGTATATA GTGAACAGAT GGAGGCCATG 
GAATTCCCTG TACCTGGGAG CTATAGAAAT ATGTTTCGCA AAAATCTCCG GGAAGGGAAG 
ACAAAACCTG AGCTCAGACA GATCATCCTT CATCATCGTA TCCGGATGCC GTCCAATCCA 
TATGCTCGCA AGATTATGGA ATTTGATACC AGAGTGAAGA TGGGGGATCA GTTTTCAGAG 
GAAGAGATTG AACTATATAA GTCCATCACC ACGACGGCCC GGAAATATGA ACTAAGCAAC 
CATGACATAA TCCTCTGTAC TTGCTCCTGT TCAGCCTCCT CCATTCTCAC CAAACAACTC 
AATGTCAAAC AAATTCTCAT TGATGAAGCT GGCATGTCCA CAGAGCCTGA AAGCCTCATC 
CCCCTTGTTA ACTTTCCCCG GGCTGAGAAG GTGGTGTTGC TAGGGGACCA CAAACAGCTT 
CAGCCTTTTG TAAAGAATGA TCACTGTCAA AACCTGGGCT TGGAGAAGTC CCTCTTCGAG 
CGTTACCATC GTCAAGCCTG GATGCTGGAC ACACAGTATC GAATGCACAA GGACATCTGC 
AAATTCCCTT CCAAAGAATT TTATGACAAT AAATTAAAGA CTAGCATGGA ACTAAGGCGA 
ACGCCAAGTG TCTTCAGCCA CCAAGATCAA GAAAGCTGTC CCATCATCTT TGGCTATGTC 
CAGGGAGAAG AGAAGAGTCT GGTAGTCTCC ACAGATGAAG GAAATGAAAA CTCCAAAGCT 
AACCAAGATG AAGTGGAGCA GGTGATTCGA ATAGCAAAGC AGCTGACCTT GGATAGGACC 
ATCAAACCCA AGGACATTGC AATCCTCAGC CCATACAATG CCCAGGTATC AGAGATCAAC 
AAGAGACTAT TAAAGGAGGG AATTACTGGA GTGACTGTCT CCTCCATCAA GAAGAGCCAA 
GGAAGTGAGT GGCGATATGT CATTCTAAGC ACAGTCCGCT CCTGCCCTCT TAGCGAAATT 
GATGGGAAGC CCACCAAAAG CTGGCTGAAG AAACACCTGG GTTTTGTCAG TGACCCACAT 
CAGGTCAATG TGGGGATCAC TCGGGCCCAA GAGGGGCTCT GCATCATTGG AAATCACTAC 
CTCTTGAGTT GCTGTCCAAT GTGGCATCGG CTCATTGATT TCTACACCAA GAAGGGATGC 
TGTGTCATGG CTTCCTCAGT GCAAGTGCGT CAGAGGCCAG CTATCTACCA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012409552.1
CDS	342..8975
Translation	MPSLKKGLLARLQSQLDQLQSQLDLRVGCSVCSEPLNESTYKWHTIEHNCPREILLARAKGAPEHKEWRKVGRRPSFPRPRRYEVCRYYKPGMGCRKHRNQCTFARSLEEALVWTFERENNIPRLWLKAKVQGSLYQGTPRILTAAEEIGAEFGGYFQELCKPCFRNCPAQVTLRGPNLPCPQHGPSPLLIVHISEGFRKKQLVEIRPRPRQGHLLEYCMYVSRGHPCRHGAVRCQYAHSDVEMAVWEAESMGCLTRSDLLVLPASLESGAGSGTSGEVGGRNGSPSAPPQIQLYCRVCLVTCSSQESFENHCSSLEHTQMLSYDHGALWEYRAPPIGLRKFKLCSRLDICEYGNGCTQAHSQEELQEWIMRAQAAQMREESARQDGLLSYQDRLLHEYQHCHNEIQVMSESIRDVTIHCDQPLKCQVEDKKSQCIWKFIIHSKDPLQHVALLKHQMGAIFSLVCPGLPQRQLYAQGSRFHVQDTAMTFQVEVLVECITFGSYEQWVAFDFGRRPVLVQKLYVQVGQKETLGPPPCIQDHNCLLELIRWHSGNRCVVPNVGRTAEQVNLLAKYKVSPQELENYRSKASDQVSITPVNYRERMHQFLYMEEEAQQELVAKLNLQVEVTLTSKMQTLAMGMKFAPSGELYAKVPIPCSLTPDTDQGYLLSRAVSTAYVAPVPAPDNHVYEVRVEVKATSEQNVWLLLPAHCCSALGLKDEDTQLMEIQFQIDKTIFGIWHQAIDALQDDRLVAPDVPACSLPSLRPPPQELRGNPKQKEAISFITGTTSGQSRVPPLLIYGPFGTGKTYTLAMATLEVIKQPNTKVLICTHTNSAADIYIREYFHAYVTAGHPEAVPLRVKNTESFLNQTDPTTLQYCCLSSDGRSFCFPTQEKLKQHRIIITTTTLSRSLCVPSGFFSHILIDEAAQMLECEAIIPLAYATQKTRIVLAGDHMQITPKLFSVGARKSADHTLLNRLFQYYQKETHKLASQSRVIFHENYRSTKAIISFVSRNFYVAKGNPIQASGQIPAHPCKYPLMFCHVAGTPERDITMTSWFNRAEIVEVIEKVQEFYRIWAYQWNSPDLKQICVVSHGSQVKILRQELRKKQLGEVAVENFENLPGREFRVIILSTVHTRDSLLNSSVPHLEFFSEARVLNTVMTRAQSQVVAVGDAVALCSFGRCSKVWKHFIQECIEMRSVSPVDLTMEQIKQDVAHRQRWARQNGLHPMNSPREHSLEEEDNDNDIPSWVSDAELNADDPILQELLDESKNVAVTVSEDGLLNVTSGSRSRYQGQQYLSFPVPVLQKYLRMQPKIYRKCELIKEAFEKASAFTIDDSPPLIIQIRGRTNCGMAFTGDQVLVEILTPELEEQGYSWRPMGRVVGILERGDRELIFICKMDEFDPRVMIPIDPSVTKIFVPELKDKPNTIPIRRLQRGHIKLISYEKITEETKRNQLFRVQIISWKERFYYPLGIILEILPLPLTLEQGLKILDMEYSLNANGRYPTPVIKEVNQHNCSKLNLHRGQWKDCRDYLTFTVDPQGSRDLDDAISVRDLGSHYEIAVHISDVASFMPKDGALDREARKRGATYYAPGKDPVAMLPSRLSQDLFSLLPQQERRTISLILTVEKESDRVVQWHITPSMICSDLQLTYEEAEVIIKSHPGGGAWLPLRLGTVEGCVVAAYHFSQVLRRSRLQGDCYYDQPDEDSPLGSRSSHQMVEELMILFNSYVAEFLVHQDNTKLITPLRCQAEPNPQQVAQLKDKYSHLLPLSLHLSYRLCGQTTSTFQGTSSGYNLCILRPLWDHLCQAADSKDYNKMVDLIATDDIHPTLAPAGLEFRKLLSRSYFSRSNSTSLSRAGHYSLQVDWYTWASSPIRRYIDVVLQRQLHAAMTKGHPGYSPEDIDLLCHDFNRKNGRAQAYERKAHCLHLATQLKTQLLTKLCFVVNVESANRFFKVIFPMNRDTLPDPHLVNYRALQLVEQPTFIPEHQKMKLVWKRRIYSLDKGKAWMPSSKTLVDTHVMPVSSVIWQDLLVAIQSQNFQKAAALLLRCRGTVQTSHHRSLGQVSRSKCSHYVEVIVELSTGDALQMQLTTDVQRGFLVPSIQMWMLTPGFAICLEHAEKPIDCFSRYASQTTKDVYSEAQEYSRVWEPLCAMESASSAVAENDSILLHGVPITWDQERNDQGQMQGSFCLTYSFLKECAIDINFNFCYLCIRLENLKLQGPVEGEEEREKGKEAALCQGFRKLSLGHTAANPSVVNTLDSCLNVDPDTYTWVAHGLTDEMDQDSKKVDRREERQRIHFFLHHVAMESIPVEVTRKEACFTIEMIPKLLPDIRKEEAIWKLKYASPLVTSIALGQPIKEVSCVAMKSSILKRQTFDIPRGKFKLNKSQNSAVLEALQKPFTLIQGPPGTGKTMVGVHIVYWFHKLNQEKPETAPLPLRGEKKDKGEKCILYCGPSNKSVDVVSEILLQWKEELRPLQVYSEQMEAMEFPVPGSYRNMFRKNLREGKTKPELRQIILHHRIRMPSNPYARKIMEFDTRVKMGDQFSEEEIELYKSITTTARKYELSNHDIILCTCSCSASSILTKQLNVKQILIDEAGMSTEPESLIPLVNFPRAEKVVLLGDHKQLQPFVKNDHCQNLGLEKSLFERYHRQAWMLDTQYRMHKDICKFPSKEFYDNKLKTSMELRRTPSVFSHQDQESCPIIFGYVQGEEKSLVVSTDEGNENSKANQDEVEQVIRIAKQLTLDRTIKPKDIAILSPYNAQVSEINKRLLKEGITGVTVSSIKKSQGSEWRYVILSTVRSCPLSEIDGKPTKSWLKKHLGFVSDPHQVNVGITRAQEGLCIIGNHYLLSCCPMWHRLIDFYTKKGCCVMASSVQVRQRPAIYH

Target ORF information:

RefSeq Version	XM_012554098.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii helicase with zinc finger 2 (HELZ2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012554098.2

ORF Insert Sequence:

ATGCCGTCAC TCAAAAAGGG GCTTCTGGCT CGGCTCCAGA GCCAACTGGA TCAGCTTCAG 
AGCCAACTGG ACCTACGTGT GGGCTGCTCA GTCTGCTCAG AGCCATTGAA TGAGAGCACA 
TATAAATGGC ATACCATAGA ACACAACTGC CCCCGTGAGA TTCTCCTGGC AAGGGCCAAG 
GGAGCTCCTG AGCATAAAGA GTGGAGAAAG GTTGGACGCA GGCCCAGCTT CCCCCGGCCC 
AGACGTTACG AAGTATGCCG CTACTACAAA CCTGGGATGG GCTGCAGGAA ACACCGCAAC 
CAGTGCACAT TTGCCCGAAG CTTAGAGGAA GCATTGGTTT GGACTTTTGA GAGGGAAAAC 
AATATCCCCA GACTGTGGCT GAAAGCTAAA GTACAGGGAA GCTTGTATCA AGGGACTCCC 
AGGATTCTGA CTGCAGCTGA AGAAATAGGG GCTGAATTTG GAGGCTACTT TCAGGAACTT 
TGTAAGCCTT GCTTCCGGAA CTGCCCTGCA CAGGTGACTC TCCGGGGCCC AAACCTGCCA 
TGCCCACAGC ATGGGCCCTC TCCCCTGCTT ATCGTACACA TAAGTGAGGG TTTTAGAAAA 
AAGCAGCTAG TGGAGATCCG GCCCCGGCCC CGGCAAGGCC ACCTACTGGA ATATTGTATG 
TACGTGTCTC GAGGACATCC GTGTCGGCAC GGAGCTGTCC GCTGCCAATA TGCTCACAGT 
GATGTGGAGA TGGCTGTGTG GGAAGCAGAA TCAATGGGCT GCCTTACTCG CTCTGACTTG 
CTTGTGCTAC CAGCCTCACT GGAATCTGGA GCAGGCTCTG GGACCAGCGG TGAGGTTGGT 
GGTAGAAATG GGAGCCCATC TGCTCCACCC CAGATTCAGC TGTATTGCCG TGTCTGTCTT 
GTAACTTGCA GCTCCCAAGA GAGTTTTGAG AACCATTGCT CATCCCTGGA ACACACACAG 
ATGTTGTCCT ATGACCATGG GGCCCTATGG GAATACCGGG CCCCCCCCAT TGGGCTCCGC 
AAATTCAAAC TTTGCAGCAG GCTAGATATT TGTGAATATG GGAATGGCTG CACTCAGGCA 
CACTCCCAGG AAGAGCTTCA AGAGTGGATT ATGCGGGCTC AGGCAGCCCA GATGAGAGAA 
GAATCGGCCC GGCAGGATGG ACTCCTCTCC TACCAGGACC GGCTTTTACA TGAATATCAA 
CACTGCCACA ATGAAATACA GGTGATGTCC GAGTCTATAC GTGATGTAAC TATTCACTGT 
GACCAGCCCT TAAAATGCCA GGTAGAGGAC AAGAAATCGC AATGCATATG GAAATTTATC 
ATCCATTCTA AGGACCCACT GCAGCACGTG GCCCTCCTCA AACATCAGAT GGGAGCCATC 
TTTTCCTTGG TGTGTCCTGG GCTTCCACAA AGACAGCTTT ATGCCCAAGG CTCAAGGTTC 
CATGTGCAGG ATACAGCGAT GACCTTCCAG GTGGAGGTGC TGGTAGAGTG TATCACCTTT 
GGCAGCTATG AACAATGGGT AGCTTTTGAC TTTGGACGCC GACCTGTCCT GGTACAGAAG 
CTATATGTTC AAGTGGGCCA AAAGGAGACC CTTGGGCCTC CCCCCTGTAT CCAGGACCAT 
AATTGTCTTC TTGAGTTGAT CCGCTGGCAC AGTGGAAATC GATGTGTGGT GCCCAATGTG 
GGCAGGACAG CTGAGCAGGT GAATCTGCTA GCTAAGTACA AAGTGAGCCC CCAGGAACTG 
GAGAACTACA GGTCAAAGGC TTCAGACCAG GTCTCTATAA CTCCAGTCAA CTATCGGGAG 
AGGATGCACC AATTCTTGTA TATGGAAGAG GAGGCTCAAC AGGAGCTGGT GGCCAAATTG 
AACCTTCAAG TCGAAGTCAC ATTGACCTCC AAGATGCAGA CCTTGGCAAT GGGCATGAAG 
TTTGCACCAT CTGGGGAATT GTATGCTAAG GTTCCAATTC CTTGTTCCCT GACACCAGAC 
ACAGACCAGG GCTATTTGCT AAGTAGAGCT GTTAGCACTG CTTACGTGGC ACCTGTCCCT 
GCCCCTGACA ATCATGTGTA TGAGGTTCGG GTGGAAGTTA AAGCCACTTC AGAGCAGAAT 
GTGTGGCTCC TACTGCCAGC CCACTGCTGC TCTGCCTTGG GACTTAAGGA TGAAGACACA 
CAGCTTATGG AAATCCAGTT TCAAATCGAC AAGACTATAT TTGGGATATG GCACCAAGCC 
ATTGATGCAC TTCAGGATGA TAGACTGGTG GCACCTGATG TACCTGCCTG TTCTCTGCCT 
TCTTTGAGGC CCCCTCCCCA GGAACTCCGT GGTAATCCCA AGCAGAAGGA GGCCATCTCC 
TTTATCACAG GGACAACGAG TGGTCAGAGC CGAGTGCCTC CACTGCTCAT TTATGGCCCC 
TTTGGGACAG GCAAGACATA CACACTAGCT ATGGCCACGC TAGAGGTTAT CAAACAGCCA 
AACACAAAAG TGTTGATCTG TACTCACACC AACAGTGCTG CTGACATCTA CATCCGTGAG 
TACTTTCATG CTTATGTCAC AGCTGGACAC CCTGAGGCTG TGCCATTGAG GGTCAAAAAC 
ACTGAAAGCT TCCTCAACCA GACAGACCCG ACCACTCTGC AGTATTGCTG CCTCTCAAGT 
GATGGACGGT CCTTTTGCTT CCCAACTCAG GAGAAACTAA AGCAGCACCG CATTATAATC 
ACTACCACTA CCTTGTCCCG CTCCCTATGT GTGCCATCTG GGTTTTTTTC CCACATTCTC 
ATTGATGAGG CTGCTCAGAT GTTAGAATGT GAAGCCATCA TCCCACTGGC CTATGCTACC 
CAGAAGACAC GCATTGTCCT TGCCGGGGAC CACATGCAAA TCACCCCCAA ACTCTTCAGT 
GTTGGGGCCA GGAAGTCTGC TGACCATACA CTGCTTAATC GGCTGTTCCA GTACTATCAG 
AAGGAGACTC ATAAATTGGC TTCCCAGAGC AGGGTCATCT TCCATGAAAA CTACCGTTCC 
ACCAAAGCCA TCATTTCCTT TGTGTCTCGG AATTTCTATG TGGCTAAGGG TAACCCTATT 
CAGGCTAGCG GACAGATCCC TGCACACCCT TGCAAGTACC CACTCATGTT TTGCCATGTG 
GCTGGTACTC CTGAGAGAGA CATAACAATG ACATCATGGT TTAACAGGGC TGAGATAGTG 
GAGGTGATTG AGAAGGTGCA AGAATTCTAT CGTATCTGGG CCTACCAATG GAATAGCCCT 
GACCTAAAGC AAATTTGTGT TGTGTCCCAT GGTTCTCAGG TAAAAATATT GAGGCAGGAA 
CTGAGGAAGA AACAACTAGG AGAAGTAGCA GTGGAGAATT TTGAAAATCT GCCAGGTCGT 
GAATTCCGAG TGATCATCCT CAGCACAGTC CACACTCGGG ACAGCCTGCT CAACAGCTCA 
GTCCCCCACC TAGAATTCTT CAGTGAGGCT CGGGTGCTGA ACACCGTGAT GACCAGAGCC 
CAGTCCCAGG TGGTTGCTGT GGGCGATGCT GTGGCCCTTT GTTCTTTTGG ACGTTGTAGC 
AAGGTATGGA AGCACTTCAT CCAAGAGTGT ATAGAGATGC GCAGTGTGTC CCCTGTGGAC 
CTCACAATGG AACAAATCAA GCAAGATGTG GCACACAGGC AGCGCTGGGC CCGGCAAAAT 
GGACTGCATC CTATGAACTC TCCCAGGGAG CACTCGTTAG AAGAGGAAGA CAATGACAAT 
GACATTCCCT CATGGGTGTC TGATGCTGAG CTCAATGCTG ATGATCCCAT CCTCCAGGAG 
TTGCTGGATG AGAGTAAGAA TGTGGCAGTG ACAGTAAGTG AGGATGGGCT GCTGAATGTG 
ACATCAGGGT CCCGGTCCCG CTATCAGGGA CAACAGTATT TAAGCTTTCC TGTGCCAGTG 
TTGCAGAAAT ATCTCCGAAT GCAACCCAAG ATATATAGGA AATGTGAACT GATAAAGGAG 
GCCTTTGAGA AGGCATCTGC CTTCACCATA GATGACTCCC CACCCCTCAT TATTCAGATC 
AGAGGACGCA CCAACTGTGG AATGGCCTTC ACTGGGGATC AAGTTCTAGT GGAAATCCTG 
ACTCCAGAAT TGGAGGAACA GGGGTACTCC TGGCGCCCCA TGGGCCGAGT GGTAGGAATA 
CTAGAAAGAG GTGACCGTGA ACTCATCTTC ATTTGCAAAA TGGATGAGTT TGACCCCCGA 
GTTATGATCC CCATTGACCC GTCAGTCACC AAGATCTTTG TGCCAGAGTT AAAGGATAAG 
CCCAACACGA TTCCTATCCG CCGCCTACAA CGAGGACACA TCAAGCTTAT TAGCTATGAG 
AAGATAACAG AGGAGACCAA GCGTAATCAG CTCTTTCGTG TTCAGATCAT CTCTTGGAAA 
GAGAGGTTCT ATTATCCCTT AGGCATCATT CTTGAGATTC TTCCCTTGCC TTTGACCTTA 
GAACAAGGTT TAAAGATTCT GGACATGGAA TATTCTCTGA ATGCTAATGG AAGATACCCA 
ACTCCAGTAA TTAAGGAAGT GAACCAACAC AACTGTAGTA AATTGAATCT GCACCGGGGA 
CAGTGGAAGG ACTGCCGGGA TTATTTGACC TTTACTGTGG ACCCGCAGGG CTCCCGGGAT 
CTGGATGATG CTATCAGTGT CCGGGACCTG GGAAGCCATT ATGAAATTGC TGTCCATATA 
TCTGATGTGG CTAGCTTCAT GCCAAAGGAT GGTGCACTTG ACCGAGAGGC TAGAAAGCGA 
GGTGCTACCT ACTATGCTCC TGGAAAGGAT CCAGTGGCCA TGCTCCCTTC CAGGCTCAGC 
CAGGACCTGT TCAGTCTCCT TCCCCAGCAA GAACGTCGCA CTATCTCCCT GATTCTTACA 
GTGGAGAAGG AAAGTGACCG AGTAGTGCAA TGGCATATCA CACCATCTAT GATATGCTCT 
GACCTCCAAC TGACGTATGA GGAGGCTGAG GTTATTATTA AGAGTCATCC TGGGGGTGGG 
GCCTGGCTGC CCCTCCGCCT GGGCACTGTA GAAGGCTGTG TTGTGGCTGC TTATCACTTC 
TCCCAAGTCC TCCGCAGAAG CCGCCTTCAG GGAGATTGCT ACTATGACCA ACCAGATGAA 
GACAGCCCTC TCGGCTCACG TAGCTCCCAC CAGATGGTTG AGGAGCTTAT GATCCTATTC 
AATAGCTATG TAGCTGAATT CCTGGTGCAC CAGGATAACA CAAAGCTGAT CACCCCATTG 
AGGTGCCAGG CTGAGCCCAA TCCTCAGCAA GTAGCTCAGT TAAAGGATAA ATACAGTCAC 
TTGCTCCCTC TGTCCCTTCA TCTCTCTTAT CGTCTCTGTG GCCAGACCAC CAGCACTTTC 
CAAGGAACCA GTTCAGGATA CAACCTTTGC ATCCTGCGCC CGCTTTGGGA CCACCTGTGC 
CAAGCTGCTG ACAGCAAGGA CTATAACAAG ATGGTGGACT TAATTGCCAC AGATGACATC 
CACCCCACTC TGGCCCCAGC TGGACTGGAG TTCCGAAAGC TGCTCAGCCG GTCCTACTTT 
AGCCGCTCCA ACTCCACCAG CCTGTCCAGG GCTGGGCATT ACTCGTTGCA GGTGGACTGG 
TATACTTGGG CATCATCCCC CATAAGGCGC TATATTGATG TGGTGCTCCA GCGGCAGCTC 
CATGCTGCCA TGACCAAGGG CCATCCTGGA TATTCCCCTG AAGATATTGA CTTACTGTGC 
CATGACTTCA ACCGGAAGAA TGGACGCGCA CAGGCTTACG AGCGCAAGGC CCACTGCCTT 
CACCTTGCCA CCCAGCTCAA GACCCAGCTG CTGACAAAGC TGTGCTTTGT GGTAAATGTG 
GAATCTGCAA ACCGCTTTTT CAAGGTGATT TTCCCCATGA ACCGGGATAC CCTGCCTGAT 
CCCCACCTTG TCAATTATCG TGCACTGCAA CTGGTAGAGC AGCCCACCTT CATCCCTGAG 
CACCAGAAAA TGAAGTTGGT TTGGAAGAGG CGAATATACT CTCTGGATAA GGGCAAAGCA 
TGGATGCCAT CCTCTAAAAC CCTGGTAGAC ACCCATGTCA TGCCAGTAAG CAGTGTCATC 
TGGCAGGACC TGCTGGTTGC AATCCAGAGT CAGAATTTTC AGAAAGCAGC AGCCTTGCTC 
CTAAGGTGTC GAGGGACTGT TCAGACCTCC CACCATCGGT CCTTAGGCCA GGTGTCTCGA 
AGCAAGTGCT CGCACTATGT AGAGGTGATA GTAGAGCTAA GTACAGGGGA TGCCCTGCAG 
ATGCAACTGA CTACTGATGT CCAGAGGGGC TTCCTTGTGC CCTCCATTCA GATGTGGATG 
TTGACCCCAG GTTTTGCCAT TTGCCTGGAG CATGCTGAGA AACCCATTGA CTGCTTTTCC 
CGCTATGCTT CACAGACAAC GAAAGATGTG TATTCTGAGG CACAGGAATA TTCCCGGGTA 
TGGGAACCAC TCTGTGCCAT GGAGTCCGCC TCCAGTGCTG TGGCTGAGAA CGACTCTATT 
CTCTTGCATG GTGTTCCCAT TACTTGGGAC CAAGAGCGGA ATGACCAGGG ACAGATGCAA 
GGTTCCTTCT GCTTAACCTA TAGCTTCCTC AAGGAATGTG CGATTGACAT CAACTTTAAT 
TTCTGCTACC TCTGCATCCG GTTGGAGAAC CTGAAACTCC AGGGTCCAGT AGAAGGGGAG 
GAGGAGAGGG AAAAAGGAAA GGAGGCTGCT CTGTGTCAAG GCTTTAGGAA GCTCAGCTTG 
GGCCACACGG CAGCAAACCC ATCTGTGGTC AATACTCTTG ACTCTTGTTT GAATGTAGAC 
CCTGACACGT ATACCTGGGT GGCTCATGGG CTGACAGATG AAATGGATCA GGATTCCAAA 
AAAGTTGATC GAAGAGAAGA ACGACAGAGA ATCCATTTTT TCCTACATCA TGTAGCCATG 
GAGAGCATTC CCGTGGAGGT CACCAGAAAG GAAGCTTGCT TCACCATTGA GATGATCCCA 
AAGCTTCTGC CAGATATCCG GAAAGAGGAA GCCATCTGGA AACTGAAATA TGCCTCACCA 
CTTGTCACCA GCATTGCCTT AGGCCAGCCC ATTAAGGAAG TGTCCTGCGT AGCCATGAAG 
TCCAGTATCC TGAAGCGCCA GACCTTTGAC ATTCCCCGAG GAAAATTCAA GCTCAATAAG 
AGTCAGAACA GTGCTGTGCT AGAAGCTCTT CAAAAGCCTT TTACCCTCAT CCAAGGGCCA 
CCAGGCACTG GGAAGACGAT GGTCGGGGTC CATATTGTAT ACTGGTTCCA TAAGCTAAAC 
CAGGAGAAGC CTGAAACTGC TCCGCTGCCC TTGAGAGGGG AGAAGAAAGA CAAAGGGGAG 
AAGTGCATCC TATATTGTGG GCCATCCAAC AAATCTGTTG ATGTTGTTTC AGAAATCCTC 
CTTCAGTGGA AGGAGGAACT CAGACCATTG CAAGTATATA GTGAACAGAT GGAGGCCATG 
GAATTCCCTG TACCTGGGAG CTATAGAAAT ATGTTTCGCA AAAATCTCCG GGAAGGGAAG 
ACAAAACCTG AGCTCAGACA GATCATCCTT CATCATCGTA TCCGGATGCC GTCCAATCCA 
TATGCTCGCA AGATTATGGA ATTTGATACC AGAGTGAAGA TGGGGGATCA GTTTTCAGAG 
GAAGAGATTG AACTATATAA GTCCATCACC ACGACGGCCC GGAAATATGA ACTAAGCAAC 
CATGACATAA TCCTCTGTAC TTGCTCCTGT TCAGCCTCCT CCATTCTCAC CAAACAACTC 
AATGTCAAAC AAATTCTCAT TGATGAAGCT GGCATGTCCA CAGAGCCTGA AAGCCTCATC 
CCCCTTGTTA ACTTTCCCCG GGCTGAGAAG GTGGTGTTGC TAGGGGACCA CAAACAGCTT 
CAGCCTTTTG TAAAGAATGA TCACTGTCAA AACCTGGGCT TGGAGAAGTC CCTCTTCGAG 
CGTTACCATC GTCAAGCCTG GATGCTGGAC ACACAGTATC GAATGCACAA GGACATCTGC 
AAATTCCCTT CCAAAGAATT TTATGACAAT AAATTAAAGA CTAGCATGGA ACTAAGGCGA 
ACGCCAAGTG TCTTCAGCCA CCAAGATCAA GAAAGCTGTC CCATCATCTT TGGCTATGTC 
CAGGGAGAAG AGAAGAGTCT GGTAGTCTCC ACAGATGAAG GAAATGAAAA CTCCAAAGCT 
AACCAAGATG AAGTGGAGCA GGTGATTCGA ATAGCAAAGC AGCTGACCTT GGATAGGACC 
ATCAAACCCA AGGACATTGC AATCCTCAGC CCATACAATG CCCAGGTATC AGAGATCAAC 
AAGAGACTAT TAAAGGAGGG AATTACTGGA GTGACTGTCT CCTCCATCAA GAAGAGCCAA 
GGAAGTGAGT GGCGATATGT CATTCTAAGC ACAGTCCGCT CCTGCCCTCT TAGCGAAATT 
GATGGGAAGC CCACCAAAAG CTGGCTGAAG AAACACCTGG GTTTTGTCAG TGACCCACAT 
CAGGTCAATG TGGGGATCAC TCGGGCCCAA GAGGGGCTCT GCATCATTGG AAATCACTAC 
CTCTTGAGTT GCTGTCCAAT GTGGCATCGG CTCATTGATT TCTACACCAA GAAGGGATGC 
TGTGTCATGG CTTCCTCAGT GCAAGTGCGT CAGAGGCCAG CTATCTACCA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HELZ2 ( XM_012554098.3 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012409552.1 Sarcophilus harrisii helicase with zinc finger 2 (HELZ2), transcript variant X1, mRNA.

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CloneID	OSa119203
Clone ID Related Accession (Same CDS sequence)	XM_012554098.2 , XM_012554098.3 , XM_012554098.1
Accession Version	XM_012554098.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	helicase with zinc finger domain 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_012554098.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCAC TCAAAAAGGG GCTTCTGGCT CGGCTCCAGA GCCAACTGGA TCAGCTTCAG 
AGCCAACTGG ACCTACGTGT GGGCTGCTCA GTCTGCTCAG AGCCATTGAA TGAGAGCACA 
TATAAATGGC ATACCATAGA ACACAACTGC CCCCGTGAGA TTCTCCTGGC AAGGGCCAAG 
GGAGCTCCTG AGCATAAAGA GTGGAGAAAG GTTGGACGCA GGCCCAGCTT CCCCCGGCCC 
AGACGTTACG AAGTATGCCG CTACTACAAA CCTGGGATGG GCTGCAGGAA ACACCGCAAC 
CAGTGCACAT TTGCCCGAAG CTTAGAGGAA GCATTGGTTT GGACTTTTGA GAGGGAAAAC 
AATATCCCCA GACTGTGGCT GAAAGCTAAA GTACAGGGAA GCTTGTATCA AGGGACTCCC 
AGGATTCTGA CTGCAGCTGA AGAAATAGGG GCTGAATTTG GAGGCTACTT TCAGGAACTT 
TGTAAGCCTT GCTTCCGGAA CTGCCCTGCA CAGGTGACTC TCCGGGGCCC AAACCTGCCA 
TGCCCACAGC ATGGGCCCTC TCCCCTGCTT ATCGTACACA TAAGTGAGGG TTTTAGAAAA 
AAGCAGCTAG TGGAGATCCG GCCCCGGCCC CGGCAAGGCC ACCTACTGGA ATATTGTATG 
TACGTGTCTC GAGGACATCC GTGTCGGCAC GGAGCTGTCC GCTGCCAATA TGCTCACAGT 
GATGTGGAGA TGGCTGTGTG GGAAGCAGAA TCAATGGGCT GCCTTACTCG CTCTGACTTG 
CTTGTGCTAC CAGCCTCACT GGAATCTGGA GCAGGCTCTG GGACCAGCGG TGAGGTTGGT 
GGTAGAAATG GGAGCCCATC TGCTCCACCC CAGATTCAGC TGTATTGCCG TGTCTGTCTT 
GTAACTTGCA GCTCCCAAGA GAGTTTTGAG AACCATTGCT CATCCCTGGA ACACACACAG 
ATGTTGTCCT ATGACCATGG GGCCCTATGG GAATACCGGG CCCCCCCCAT TGGGCTCCGC 
AAATTCAAAC TTTGCAGCAG GCTAGATATT TGTGAATATG GGAATGGCTG CACTCAGGCA 
CACTCCCAGG AAGAGCTTCA AGAGTGGATT ATGCGGGCTC AGGCAGCCCA GATGAGAGAA 
GAATCGGCCC GGCAGGATGG ACTCCTCTCC TACCAGGACC GGCTTTTACA TGAATATCAA 
CACTGCCACA ATGAAATACA GGTGATGTCC GAGTCTATAC GTGATGTAAC TATTCACTGT 
GACCAGCCCT TAAAATGCCA GGTAGAGGAC AAGAAATCGC AATGCATATG GAAATTTATC 
ATCCATTCTA AGGACCCACT GCAGCACGTG GCCCTCCTCA AACATCAGAT GGGAGCCATC 
TTTTCCTTGG TGTGTCCTGG GCTTCCACAA AGACAGCTTT ATGCCCAAGG CTCAAGGTTC 
CATGTGCAGG ATACAGCGAT GACCTTCCAG GTGGAGGTGC TGGTAGAGTG TATCACCTTT 
GGCAGCTATG AACAATGGGT AGCTTTTGAC TTTGGACGCC GACCTGTCCT GGTACAGAAG 
CTATATGTTC AAGTGGGCCA AAAGGAGACC CTTGGGCCTC CCCCCTGTAT CCAGGACCAT 
AATTGTCTTC TTGAGTTGAT CCGCTGGCAC AGTGGAAATC GATGTGTGGT GCCCAATGTG 
GGCAGGACAG CTGAGCAGGT GAATCTGCTA GCTAAGTACA AAGTGAGCCC CCAGGAACTG 
GAGAACTACA GGTCAAAGGC TTCAGACCAG GTCTCTATAA CTCCAGTCAA CTATCGGGAG 
AGGATGCACC AATTCTTGTA TATGGAAGAG GAGGCTCAAC AGGAGCTGGT GGCCAAATTG 
AACCTTCAAG TCGAAGTCAC ATTGACCTCC AAGATGCAGA CCTTGGCAAT GGGCATGAAG 
TTTGCACCAT CTGGGGAATT GTATGCTAAG GTTCCAATTC CTTGTTCCCT GACACCAGAC 
ACAGACCAGG GCTATTTGCT AAGTAGAGCT GTTAGCACTG CTTACGTGGC ACCTGTCCCT 
GCCCCTGACA ATCATGTGTA TGAGGTTCGG GTGGAAGTTA AAGCCACTTC AGAGCAGAAT 
GTGTGGCTCC TACTGCCAGC CCACTGCTGC TCTGCCTTGG GACTTAAGGA TGAAGACACA 
CAGCTTATGG AAATCCAGTT TCAAATCGAC AAGACTATAT TTGGGATATG GCACCAAGCC 
ATTGATGCAC TTCAGGATGA TAGACTGGTG GCACCTGATG TACCTGCCTG TTCTCTGCCT 
TCTTTGAGGC CCCCTCCCCA GGAACTCCGT GGTAATCCCA AGCAGAAGGA GGCCATCTCC 
TTTATCACAG GGACAACGAG TGGTCAGAGC CGAGTGCCTC CACTGCTCAT TTATGGCCCC 
TTTGGGACAG GCAAGACATA CACACTAGCT ATGGCCACGC TAGAGGTTAT CAAACAGCCA 
AACACAAAAG TGTTGATCTG TACTCACACC AACAGTGCTG CTGACATCTA CATCCGTGAG 
TACTTTCATG CTTATGTCAC AGCTGGACAC CCTGAGGCTG TGCCATTGAG GGTCAAAAAC 
ACTGAAAGCT TCCTCAACCA GACAGACCCG ACCACTCTGC AGTATTGCTG CCTCTCAAGT 
GATGGACGGT CCTTTTGCTT CCCAACTCAG GAGAAACTAA AGCAGCACCG CATTATAATC 
ACTACCACTA CCTTGTCCCG CTCCCTATGT GTGCCATCTG GGTTTTTTTC CCACATTCTC 
ATTGATGAGG CTGCTCAGAT GTTAGAATGT GAAGCCATCA TCCCACTGGC CTATGCTACC 
CAGAAGACAC GCATTGTCCT TGCCGGGGAC CACATGCAAA TCACCCCCAA ACTCTTCAGT 
GTTGGGGCCA GGAAGTCTGC TGACCATACA CTGCTTAATC GGCTGTTCCA GTACTATCAG 
AAGGAGACTC ATAAATTGGC TTCCCAGAGC AGGGTCATCT TCCATGAAAA CTACCGTTCC 
ACCAAAGCCA TCATTTCCTT TGTGTCTCGG AATTTCTATG TGGCTAAGGG TAACCCTATT 
CAGGCTAGCG GACAGATCCC TGCACACCCT TGCAAGTACC CACTCATGTT TTGCCATGTG 
GCTGGTACTC CTGAGAGAGA CATAACAATG ACATCATGGT TTAACAGGGC TGAGATAGTG 
GAGGTGATTG AGAAGGTGCA AGAATTCTAT CGTATCTGGG CCTACCAATG GAATAGCCCT 
GACCTAAAGC AAATTTGTGT TGTGTCCCAT GGTTCTCAGG TAAAAATATT GAGGCAGGAA 
CTGAGGAAGA AACAACTAGG AGAAGTAGCA GTGGAGAATT TTGAAAATCT GCCAGGTCGT 
GAATTCCGAG TGATCATCCT CAGCACAGTC CACACTCGGG ACAGCCTGCT CAACAGCTCA 
GTCCCCCACC TAGAATTCTT CAGTGAGGCT CGGGTGCTGA ACACCGTGAT GACCAGAGCC 
CAGTCCCAGG TGGTTGCTGT GGGCGATGCT GTGGCCCTTT GTTCTTTTGG ACGTTGTAGC 
AAGGTATGGA AGCACTTCAT CCAAGAGTGT ATAGAGATGC GCAGTGTGTC CCCTGTGGAC 
CTCACAATGG AACAAATCAA GCAAGATGTG GCACACAGGC AGCGCTGGGC CCGGCAAAAT 
GGACTGCATC CTATGAACTC TCCCAGGGAG CACTCGTTAG AAGAGGAAGA CAATGACAAT 
GACATTCCCT CATGGGTGTC TGATGCTGAG CTCAATGCTG ATGATCCCAT CCTCCAGGAG 
TTGCTGGATG AGAGTAAGAA TGTGGCAGTG ACAGTAAGTG AGGATGGGCT GCTGAATGTG 
ACATCAGGGT CCCGGTCCCG CTATCAGGGA CAACAGTATT TAAGCTTTCC TGTGCCAGTG 
TTGCAGAAAT ATCTCCGAAT GCAACCCAAG ATATATAGGA AATGTGAACT GATAAAGGAG 
GCCTTTGAGA AGGCATCTGC CTTCACCATA GATGACTCCC CACCCCTCAT TATTCAGATC 
AGAGGACGCA CCAACTGTGG AATGGCCTTC ACTGGGGATC AAGTTCTAGT GGAAATCCTG 
ACTCCAGAAT TGGAGGAACA GGGGTACTCC TGGCGCCCCA TGGGCCGAGT GGTAGGAATA 
CTAGAAAGAG GTGACCGTGA ACTCATCTTC ATTTGCAAAA TGGATGAGTT TGACCCCCGA 
GTTATGATCC CCATTGACCC GTCAGTCACC AAGATCTTTG TGCCAGAGTT AAAGGATAAG 
CCCAACACGA TTCCTATCCG CCGCCTACAA CGAGGACACA TCAAGCTTAT TAGCTATGAG 
AAGATAACAG AGGAGACCAA GCGTAATCAG CTCTTTCGTG TTCAGATCAT CTCTTGGAAA 
GAGAGGTTCT ATTATCCCTT AGGCATCATT CTTGAGATTC TTCCCTTGCC TTTGACCTTA 
GAACAAGGTT TAAAGATTCT GGACATGGAA TATTCTCTGA ATGCTAATGG AAGATACCCA 
ACTCCAGTAA TTAAGGAAGT GAACCAACAC AACTGTAGTA AATTGAATCT GCACCGGGGA 
CAGTGGAAGG ACTGCCGGGA TTATTTGACC TTTACTGTGG ACCCGCAGGG CTCCCGGGAT 
CTGGATGATG CTATCAGTGT CCGGGACCTG GGAAGCCATT ATGAAATTGC TGTCCATATA 
TCTGATGTGG CTAGCTTCAT GCCAAAGGAT GGTGCACTTG ACCGAGAGGC TAGAAAGCGA 
GGTGCTACCT ACTATGCTCC TGGAAAGGAT CCAGTGGCCA TGCTCCCTTC CAGGCTCAGC 
CAGGACCTGT TCAGTCTCCT TCCCCAGCAA GAACGTCGCA CTATCTCCCT GATTCTTACA 
GTGGAGAAGG AAAGTGACCG AGTAGTGCAA TGGCATATCA CACCATCTAT GATATGCTCT 
GACCTCCAAC TGACGTATGA GGAGGCTGAG GTTATTATTA AGAGTCATCC TGGGGGTGGG 
GCCTGGCTGC CCCTCCGCCT GGGCACTGTA GAAGGCTGTG TTGTGGCTGC TTATCACTTC 
TCCCAAGTCC TCCGCAGAAG CCGCCTTCAG GGAGATTGCT ACTATGACCA ACCAGATGAA 
GACAGCCCTC TCGGCTCACG TAGCTCCCAC CAGATGGTTG AGGAGCTTAT GATCCTATTC 
AATAGCTATG TAGCTGAATT CCTGGTGCAC CAGGATAACA CAAAGCTGAT CACCCCATTG 
AGGTGCCAGG CTGAGCCCAA TCCTCAGCAA GTAGCTCAGT TAAAGGATAA ATACAGTCAC 
TTGCTCCCTC TGTCCCTTCA TCTCTCTTAT CGTCTCTGTG GCCAGACCAC CAGCACTTTC 
CAAGGAACCA GTTCAGGATA CAACCTTTGC ATCCTGCGCC CGCTTTGGGA CCACCTGTGC 
CAAGCTGCTG ACAGCAAGGA CTATAACAAG ATGGTGGACT TAATTGCCAC AGATGACATC 
CACCCCACTC TGGCCCCAGC TGGACTGGAG TTCCGAAAGC TGCTCAGCCG GTCCTACTTT 
AGCCGCTCCA ACTCCACCAG CCTGTCCAGG GCTGGGCATT ACTCGTTGCA GGTGGACTGG 
TATACTTGGG CATCATCCCC CATAAGGCGC TATATTGATG TGGTGCTCCA GCGGCAGCTC 
CATGCTGCCA TGACCAAGGG CCATCCTGGA TATTCCCCTG AAGATATTGA CTTACTGTGC 
CATGACTTCA ACCGGAAGAA TGGACGCGCA CAGGCTTACG AGCGCAAGGC CCACTGCCTT 
CACCTTGCCA CCCAGCTCAA GACCCAGCTG CTGACAAAGC TGTGCTTTGT GGTAAATGTG 
GAATCTGCAA ACCGCTTTTT CAAGGTGATT TTCCCCATGA ACCGGGATAC CCTGCCTGAT 
CCCCACCTTG TCAATTATCG TGCACTGCAA CTGGTAGAGC AGCCCACCTT CATCCCTGAG 
CACCAGAAAA TGAAGTTGGT TTGGAAGAGG CGAATATACT CTCTGGATAA GGGCAAAGCA 
TGGATGCCAT CCTCTAAAAC CCTGGTAGAC ACCCATGTCA TGCCAGTAAG CAGTGTCATC 
TGGCAGGACC TGCTGGTTGC AATCCAGAGT CAGAATTTTC AGAAAGCAGC AGCCTTGCTC 
CTAAGGTGTC GAGGGACTGT TCAGACCTCC CACCATCGGT CCTTAGGCCA GGTGTCTCGA 
AGCAAGTGCT CGCACTATGT AGAGGTGATA GTAGAGCTAA GTACAGGGGA TGCCCTGCAG 
ATGCAACTGA CTACTGATGT CCAGAGGGGC TTCCTTGTGC CCTCCATTCA GATGTGGATG 
TTGACCCCAG GTTTTGCCAT TTGCCTGGAG CATGCTGAGA AACCCATTGA CTGCTTTTCC 
CGCTATGCTT CACAGACAAC GAAAGATGTG TATTCTGAGG CACAGGAATA TTCCCGGGTA 
TGGGAACCAC TCTGTGCCAT GGAGTCCGCC TCCAGTGCTG TGGCTGAGAA CGACTCTATT 
CTCTTGCATG GTGTTCCCAT TACTTGGGAC CAAGAGCGGA ATGACCAGGG ACAGATGCAA 
GGTTCCTTCT GCTTAACCTA TAGCTTCCTC AAGGAATGTG CGATTGACAT CAACTTTAAT 
TTCTGCTACC TCTGCATCCG GTTGGAGAAC CTGAAACTCC AGGGTCCAGT AGAAGGGGAG 
GAGGAGAGGG AAAAAGGAAA GGAGGCTGCT CTGTGTCAAG GCTTTAGGAA GCTCAGCTTG 
GGCCACACGG CAGCAAACCC ATCTGTGGTC AATACTCTTG ACTCTTGTTT GAATGTAGAC 
CCTGACACGT ATACCTGGGT GGCTCATGGG CTGACAGATG AAATGGATCA GGATTCCAAA 
AAAGTTGATC GAAGAGAAGA ACGACAGAGA ATCCATTTTT TCCTACATCA TGTAGCCATG 
GAGAGCATTC CCGTGGAGGT CACCAGAAAG GAAGCTTGCT TCACCATTGA GATGATCCCA 
AAGCTTCTGC CAGATATCCG GAAAGAGGAA GCCATCTGGA AACTGAAATA TGCCTCACCA 
CTTGTCACCA GCATTGCCTT AGGCCAGCCC ATTAAGGAAG TGTCCTGCGT AGCCATGAAG 
TCCAGTATCC TGAAGCGCCA GACCTTTGAC ATTCCCCGAG GAAAATTCAA GCTCAATAAG 
AGTCAGAACA GTGCTGTGCT AGAAGCTCTT CAAAAGCCTT TTACCCTCAT CCAAGGGCCA 
CCAGGCACTG GGAAGACGAT GGTCGGGGTC CATATTGTAT ACTGGTTCCA TAAGCTAAAC 
CAGGAGAAGC CTGAAACTGC TCCGCTGCCC TTGAGAGGGG AGAAGAAAGA CAAAGGGGAG 
AAGTGCATCC TATATTGTGG GCCATCCAAC AAATCTGTTG ATGTTGTTTC AGAAATCCTC 
CTTCAGTGGA AGGAGGAACT CAGACCATTG CAAGTATATA GTGAACAGAT GGAGGCCATG 
GAATTCCCTG TACCTGGGAG CTATAGAAAT ATGTTTCGCA AAAATCTCCG GGAAGGGAAG 
ACAAAACCTG AGCTCAGACA GATCATCCTT CATCATCGTA TCCGGATGCC GTCCAATCCA 
TATGCTCGCA AGATTATGGA ATTTGATACC AGAGTGAAGA TGGGGGATCA GTTTTCAGAG 
GAAGAGATTG AACTATATAA GTCCATCACC ACGACGGCCC GGAAATATGA ACTAAGCAAC 
CATGACATAA TCCTCTGTAC TTGCTCCTGT TCAGCCTCCT CCATTCTCAC CAAACAACTC 
AATGTCAAAC AAATTCTCAT TGATGAAGCT GGCATGTCCA CAGAGCCTGA AAGCCTCATC 
CCCCTTGTTA ACTTTCCCCG GGCTGAGAAG GTGGTGTTGC TAGGGGACCA CAAACAGCTT 
CAGCCTTTTG TAAAGAATGA TCACTGTCAA AACCTGGGCT TGGAGAAGTC CCTCTTCGAG 
CGTTACCATC GTCAAGCCTG GATGCTGGAC ACACAGTATC GAATGCACAA GGACATCTGC 
AAATTCCCTT CCAAAGAATT TTATGACAAT AAATTAAAGA CTAGCATGGA ACTAAGGCGA 
ACGCCAAGTG TCTTCAGCCA CCAAGATCAA GAAAGCTGTC CCATCATCTT TGGCTATGTC 
CAGGGAGAAG AGAAGAGTCT GGTAGTCTCC ACAGATGAAG GAAATGAAAA CTCCAAAGCT 
AACCAAGATG AAGTGGAGCA GGTGATTCGA ATAGCAAAGC AGCTGACCTT GGATAGGACC 
ATCAAACCCA AGGACATTGC AATCCTCAGC CCATACAATG CCCAGGTATC AGAGATCAAC 
AAGAGACTAT TAAAGGAGGG AATTACTGGA GTGACTGTCT CCTCCATCAA GAAGAGCCAA 
GGAAGTGAGT GGCGATATGT CATTCTAAGC ACAGTCCGCT CCTGCCCTCT TAGCGAAATT 
GATGGGAAGC CCACCAAAAG CTGGCTGAAG AAACACCTGG GTTTTGTCAG TGACCCACAT 
CAGGTCAATG TGGGGATCAC TCGGGCCCAA GAGGGGCTCT GCATCATTGG AAATCACTAC 
CTCTTGAGTT GCTGTCCAAT GTGGCATCGG CTCATTGATT TCTACACCAA GAAGGGATGC 
TGTGTCATGG CTTCCTCAGT GCAAGTGCGT CAGAGGCCAG CTATCTACCA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012409552.1
CDS	381..9014
Translation	MPSLKKGLLARLQSQLDQLQSQLDLRVGCSVCSEPLNESTYKWHTIEHNCPREILLARAKGAPEHKEWRKVGRRPSFPRPRRYEVCRYYKPGMGCRKHRNQCTFARSLEEALVWTFERENNIPRLWLKAKVQGSLYQGTPRILTAAEEIGAEFGGYFQELCKPCFRNCPAQVTLRGPNLPCPQHGPSPLLIVHISEGFRKKQLVEIRPRPRQGHLLEYCMYVSRGHPCRHGAVRCQYAHSDVEMAVWEAESMGCLTRSDLLVLPASLESGAGSGTSGEVGGRNGSPSAPPQIQLYCRVCLVTCSSQESFENHCSSLEHTQMLSYDHGALWEYRAPPIGLRKFKLCSRLDICEYGNGCTQAHSQEELQEWIMRAQAAQMREESARQDGLLSYQDRLLHEYQHCHNEIQVMSESIRDVTIHCDQPLKCQVEDKKSQCIWKFIIHSKDPLQHVALLKHQMGAIFSLVCPGLPQRQLYAQGSRFHVQDTAMTFQVEVLVECITFGSYEQWVAFDFGRRPVLVQKLYVQVGQKETLGPPPCIQDHNCLLELIRWHSGNRCVVPNVGRTAEQVNLLAKYKVSPQELENYRSKASDQVSITPVNYRERMHQFLYMEEEAQQELVAKLNLQVEVTLTSKMQTLAMGMKFAPSGELYAKVPIPCSLTPDTDQGYLLSRAVSTAYVAPVPAPDNHVYEVRVEVKATSEQNVWLLLPAHCCSALGLKDEDTQLMEIQFQIDKTIFGIWHQAIDALQDDRLVAPDVPACSLPSLRPPPQELRGNPKQKEAISFITGTTSGQSRVPPLLIYGPFGTGKTYTLAMATLEVIKQPNTKVLICTHTNSAADIYIREYFHAYVTAGHPEAVPLRVKNTESFLNQTDPTTLQYCCLSSDGRSFCFPTQEKLKQHRIIITTTTLSRSLCVPSGFFSHILIDEAAQMLECEAIIPLAYATQKTRIVLAGDHMQITPKLFSVGARKSADHTLLNRLFQYYQKETHKLASQSRVIFHENYRSTKAIISFVSRNFYVAKGNPIQASGQIPAHPCKYPLMFCHVAGTPERDITMTSWFNRAEIVEVIEKVQEFYRIWAYQWNSPDLKQICVVSHGSQVKILRQELRKKQLGEVAVENFENLPGREFRVIILSTVHTRDSLLNSSVPHLEFFSEARVLNTVMTRAQSQVVAVGDAVALCSFGRCSKVWKHFIQECIEMRSVSPVDLTMEQIKQDVAHRQRWARQNGLHPMNSPREHSLEEEDNDNDIPSWVSDAELNADDPILQELLDESKNVAVTVSEDGLLNVTSGSRSRYQGQQYLSFPVPVLQKYLRMQPKIYRKCELIKEAFEKASAFTIDDSPPLIIQIRGRTNCGMAFTGDQVLVEILTPELEEQGYSWRPMGRVVGILERGDRELIFICKMDEFDPRVMIPIDPSVTKIFVPELKDKPNTIPIRRLQRGHIKLISYEKITEETKRNQLFRVQIISWKERFYYPLGIILEILPLPLTLEQGLKILDMEYSLNANGRYPTPVIKEVNQHNCSKLNLHRGQWKDCRDYLTFTVDPQGSRDLDDAISVRDLGSHYEIAVHISDVASFMPKDGALDREARKRGATYYAPGKDPVAMLPSRLSQDLFSLLPQQERRTISLILTVEKESDRVVQWHITPSMICSDLQLTYEEAEVIIKSHPGGGAWLPLRLGTVEGCVVAAYHFSQVLRRSRLQGDCYYDQPDEDSPLGSRSSHQMVEELMILFNSYVAEFLVHQDNTKLITPLRCQAEPNPQQVAQLKDKYSHLLPLSLHLSYRLCGQTTSTFQGTSSGYNLCILRPLWDHLCQAADSKDYNKMVDLIATDDIHPTLAPAGLEFRKLLSRSYFSRSNSTSLSRAGHYSLQVDWYTWASSPIRRYIDVVLQRQLHAAMTKGHPGYSPEDIDLLCHDFNRKNGRAQAYERKAHCLHLATQLKTQLLTKLCFVVNVESANRFFKVIFPMNRDTLPDPHLVNYRALQLVEQPTFIPEHQKMKLVWKRRIYSLDKGKAWMPSSKTLVDTHVMPVSSVIWQDLLVAIQSQNFQKAAALLLRCRGTVQTSHHRSLGQVSRSKCSHYVEVIVELSTGDALQMQLTTDVQRGFLVPSIQMWMLTPGFAICLEHAEKPIDCFSRYASQTTKDVYSEAQEYSRVWEPLCAMESASSAVAENDSILLHGVPITWDQERNDQGQMQGSFCLTYSFLKECAIDINFNFCYLCIRLENLKLQGPVEGEEEREKGKEAALCQGFRKLSLGHTAANPSVVNTLDSCLNVDPDTYTWVAHGLTDEMDQDSKKVDRREERQRIHFFLHHVAMESIPVEVTRKEACFTIEMIPKLLPDIRKEEAIWKLKYASPLVTSIALGQPIKEVSCVAMKSSILKRQTFDIPRGKFKLNKSQNSAVLEALQKPFTLIQGPPGTGKTMVGVHIVYWFHKLNQEKPETAPLPLRGEKKDKGEKCILYCGPSNKSVDVVSEILLQWKEELRPLQVYSEQMEAMEFPVPGSYRNMFRKNLREGKTKPELRQIILHHRIRMPSNPYARKIMEFDTRVKMGDQFSEEEIELYKSITTTARKYELSNHDIILCTCSCSASSILTKQLNVKQILIDEAGMSTEPESLIPLVNFPRAEKVVLLGDHKQLQPFVKNDHCQNLGLEKSLFERYHRQAWMLDTQYRMHKDICKFPSKEFYDNKLKTSMELRRTPSVFSHQDQESCPIIFGYVQGEEKSLVVSTDEGNENSKANQDEVEQVIRIAKQLTLDRTIKPKDIAILSPYNAQVSEINKRLLKEGITGVTVSSIKKSQGSEWRYVILSTVRSCPLSEIDGKPTKSWLKKHLGFVSDPHQVNVGITRAQEGLCIIGNHYLLSCCPMWHRLIDFYTKKGCCVMASSVQVRQRPAIYH

Target ORF information:

RefSeq Version	XM_012554098.3
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii helicase with zinc finger 2 (HELZ2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012554098.3

ORF Insert Sequence:

ATGCCGTCAC TCAAAAAGGG GCTTCTGGCT CGGCTCCAGA GCCAACTGGA TCAGCTTCAG 
AGCCAACTGG ACCTACGTGT GGGCTGCTCA GTCTGCTCAG AGCCATTGAA TGAGAGCACA 
TATAAATGGC ATACCATAGA ACACAACTGC CCCCGTGAGA TTCTCCTGGC AAGGGCCAAG 
GGAGCTCCTG AGCATAAAGA GTGGAGAAAG GTTGGACGCA GGCCCAGCTT CCCCCGGCCC 
AGACGTTACG AAGTATGCCG CTACTACAAA CCTGGGATGG GCTGCAGGAA ACACCGCAAC 
CAGTGCACAT TTGCCCGAAG CTTAGAGGAA GCATTGGTTT GGACTTTTGA GAGGGAAAAC 
AATATCCCCA GACTGTGGCT GAAAGCTAAA GTACAGGGAA GCTTGTATCA AGGGACTCCC 
AGGATTCTGA CTGCAGCTGA AGAAATAGGG GCTGAATTTG GAGGCTACTT TCAGGAACTT 
TGTAAGCCTT GCTTCCGGAA CTGCCCTGCA CAGGTGACTC TCCGGGGCCC AAACCTGCCA 
TGCCCACAGC ATGGGCCCTC TCCCCTGCTT ATCGTACACA TAAGTGAGGG TTTTAGAAAA 
AAGCAGCTAG TGGAGATCCG GCCCCGGCCC CGGCAAGGCC ACCTACTGGA ATATTGTATG 
TACGTGTCTC GAGGACATCC GTGTCGGCAC GGAGCTGTCC GCTGCCAATA TGCTCACAGT 
GATGTGGAGA TGGCTGTGTG GGAAGCAGAA TCAATGGGCT GCCTTACTCG CTCTGACTTG 
CTTGTGCTAC CAGCCTCACT GGAATCTGGA GCAGGCTCTG GGACCAGCGG TGAGGTTGGT 
GGTAGAAATG GGAGCCCATC TGCTCCACCC CAGATTCAGC TGTATTGCCG TGTCTGTCTT 
GTAACTTGCA GCTCCCAAGA GAGTTTTGAG AACCATTGCT CATCCCTGGA ACACACACAG 
ATGTTGTCCT ATGACCATGG GGCCCTATGG GAATACCGGG CCCCCCCCAT TGGGCTCCGC 
AAATTCAAAC TTTGCAGCAG GCTAGATATT TGTGAATATG GGAATGGCTG CACTCAGGCA 
CACTCCCAGG AAGAGCTTCA AGAGTGGATT ATGCGGGCTC AGGCAGCCCA GATGAGAGAA 
GAATCGGCCC GGCAGGATGG ACTCCTCTCC TACCAGGACC GGCTTTTACA TGAATATCAA 
CACTGCCACA ATGAAATACA GGTGATGTCC GAGTCTATAC GTGATGTAAC TATTCACTGT 
GACCAGCCCT TAAAATGCCA GGTAGAGGAC AAGAAATCGC AATGCATATG GAAATTTATC 
ATCCATTCTA AGGACCCACT GCAGCACGTG GCCCTCCTCA AACATCAGAT GGGAGCCATC 
TTTTCCTTGG TGTGTCCTGG GCTTCCACAA AGACAGCTTT ATGCCCAAGG CTCAAGGTTC 
CATGTGCAGG ATACAGCGAT GACCTTCCAG GTGGAGGTGC TGGTAGAGTG TATCACCTTT 
GGCAGCTATG AACAATGGGT AGCTTTTGAC TTTGGACGCC GACCTGTCCT GGTACAGAAG 
CTATATGTTC AAGTGGGCCA AAAGGAGACC CTTGGGCCTC CCCCCTGTAT CCAGGACCAT 
AATTGTCTTC TTGAGTTGAT CCGCTGGCAC AGTGGAAATC GATGTGTGGT GCCCAATGTG 
GGCAGGACAG CTGAGCAGGT GAATCTGCTA GCTAAGTACA AAGTGAGCCC CCAGGAACTG 
GAGAACTACA GGTCAAAGGC TTCAGACCAG GTCTCTATAA CTCCAGTCAA CTATCGGGAG 
AGGATGCACC AATTCTTGTA TATGGAAGAG GAGGCTCAAC AGGAGCTGGT GGCCAAATTG 
AACCTTCAAG TCGAAGTCAC ATTGACCTCC AAGATGCAGA CCTTGGCAAT GGGCATGAAG 
TTTGCACCAT CTGGGGAATT GTATGCTAAG GTTCCAATTC CTTGTTCCCT GACACCAGAC 
ACAGACCAGG GCTATTTGCT AAGTAGAGCT GTTAGCACTG CTTACGTGGC ACCTGTCCCT 
GCCCCTGACA ATCATGTGTA TGAGGTTCGG GTGGAAGTTA AAGCCACTTC AGAGCAGAAT 
GTGTGGCTCC TACTGCCAGC CCACTGCTGC TCTGCCTTGG GACTTAAGGA TGAAGACACA 
CAGCTTATGG AAATCCAGTT TCAAATCGAC AAGACTATAT TTGGGATATG GCACCAAGCC 
ATTGATGCAC TTCAGGATGA TAGACTGGTG GCACCTGATG TACCTGCCTG TTCTCTGCCT 
TCTTTGAGGC CCCCTCCCCA GGAACTCCGT GGTAATCCCA AGCAGAAGGA GGCCATCTCC 
TTTATCACAG GGACAACGAG TGGTCAGAGC CGAGTGCCTC CACTGCTCAT TTATGGCCCC 
TTTGGGACAG GCAAGACATA CACACTAGCT ATGGCCACGC TAGAGGTTAT CAAACAGCCA 
AACACAAAAG TGTTGATCTG TACTCACACC AACAGTGCTG CTGACATCTA CATCCGTGAG 
TACTTTCATG CTTATGTCAC AGCTGGACAC CCTGAGGCTG TGCCATTGAG GGTCAAAAAC 
ACTGAAAGCT TCCTCAACCA GACAGACCCG ACCACTCTGC AGTATTGCTG CCTCTCAAGT 
GATGGACGGT CCTTTTGCTT CCCAACTCAG GAGAAACTAA AGCAGCACCG CATTATAATC 
ACTACCACTA CCTTGTCCCG CTCCCTATGT GTGCCATCTG GGTTTTTTTC CCACATTCTC 
ATTGATGAGG CTGCTCAGAT GTTAGAATGT GAAGCCATCA TCCCACTGGC CTATGCTACC 
CAGAAGACAC GCATTGTCCT TGCCGGGGAC CACATGCAAA TCACCCCCAA ACTCTTCAGT 
GTTGGGGCCA GGAAGTCTGC TGACCATACA CTGCTTAATC GGCTGTTCCA GTACTATCAG 
AAGGAGACTC ATAAATTGGC TTCCCAGAGC AGGGTCATCT TCCATGAAAA CTACCGTTCC 
ACCAAAGCCA TCATTTCCTT TGTGTCTCGG AATTTCTATG TGGCTAAGGG TAACCCTATT 
CAGGCTAGCG GACAGATCCC TGCACACCCT TGCAAGTACC CACTCATGTT TTGCCATGTG 
GCTGGTACTC CTGAGAGAGA CATAACAATG ACATCATGGT TTAACAGGGC TGAGATAGTG 
GAGGTGATTG AGAAGGTGCA AGAATTCTAT CGTATCTGGG CCTACCAATG GAATAGCCCT 
GACCTAAAGC AAATTTGTGT TGTGTCCCAT GGTTCTCAGG TAAAAATATT GAGGCAGGAA 
CTGAGGAAGA AACAACTAGG AGAAGTAGCA GTGGAGAATT TTGAAAATCT GCCAGGTCGT 
GAATTCCGAG TGATCATCCT CAGCACAGTC CACACTCGGG ACAGCCTGCT CAACAGCTCA 
GTCCCCCACC TAGAATTCTT CAGTGAGGCT CGGGTGCTGA ACACCGTGAT GACCAGAGCC 
CAGTCCCAGG TGGTTGCTGT GGGCGATGCT GTGGCCCTTT GTTCTTTTGG ACGTTGTAGC 
AAGGTATGGA AGCACTTCAT CCAAGAGTGT ATAGAGATGC GCAGTGTGTC CCCTGTGGAC 
CTCACAATGG AACAAATCAA GCAAGATGTG GCACACAGGC AGCGCTGGGC CCGGCAAAAT 
GGACTGCATC CTATGAACTC TCCCAGGGAG CACTCGTTAG AAGAGGAAGA CAATGACAAT 
GACATTCCCT CATGGGTGTC TGATGCTGAG CTCAATGCTG ATGATCCCAT CCTCCAGGAG 
TTGCTGGATG AGAGTAAGAA TGTGGCAGTG ACAGTAAGTG AGGATGGGCT GCTGAATGTG 
ACATCAGGGT CCCGGTCCCG CTATCAGGGA CAACAGTATT TAAGCTTTCC TGTGCCAGTG 
TTGCAGAAAT ATCTCCGAAT GCAACCCAAG ATATATAGGA AATGTGAACT GATAAAGGAG 
GCCTTTGAGA AGGCATCTGC CTTCACCATA GATGACTCCC CACCCCTCAT TATTCAGATC 
AGAGGACGCA CCAACTGTGG AATGGCCTTC ACTGGGGATC AAGTTCTAGT GGAAATCCTG 
ACTCCAGAAT TGGAGGAACA GGGGTACTCC TGGCGCCCCA TGGGCCGAGT GGTAGGAATA 
CTAGAAAGAG GTGACCGTGA ACTCATCTTC ATTTGCAAAA TGGATGAGTT TGACCCCCGA 
GTTATGATCC CCATTGACCC GTCAGTCACC AAGATCTTTG TGCCAGAGTT AAAGGATAAG 
CCCAACACGA TTCCTATCCG CCGCCTACAA CGAGGACACA TCAAGCTTAT TAGCTATGAG 
AAGATAACAG AGGAGACCAA GCGTAATCAG CTCTTTCGTG TTCAGATCAT CTCTTGGAAA 
GAGAGGTTCT ATTATCCCTT AGGCATCATT CTTGAGATTC TTCCCTTGCC TTTGACCTTA 
GAACAAGGTT TAAAGATTCT GGACATGGAA TATTCTCTGA ATGCTAATGG AAGATACCCA 
ACTCCAGTAA TTAAGGAAGT GAACCAACAC AACTGTAGTA AATTGAATCT GCACCGGGGA 
CAGTGGAAGG ACTGCCGGGA TTATTTGACC TTTACTGTGG ACCCGCAGGG CTCCCGGGAT 
CTGGATGATG CTATCAGTGT CCGGGACCTG GGAAGCCATT ATGAAATTGC TGTCCATATA 
TCTGATGTGG CTAGCTTCAT GCCAAAGGAT GGTGCACTTG ACCGAGAGGC TAGAAAGCGA 
GGTGCTACCT ACTATGCTCC TGGAAAGGAT CCAGTGGCCA TGCTCCCTTC CAGGCTCAGC 
CAGGACCTGT TCAGTCTCCT TCCCCAGCAA GAACGTCGCA CTATCTCCCT GATTCTTACA 
GTGGAGAAGG AAAGTGACCG AGTAGTGCAA TGGCATATCA CACCATCTAT GATATGCTCT 
GACCTCCAAC TGACGTATGA GGAGGCTGAG GTTATTATTA AGAGTCATCC TGGGGGTGGG 
GCCTGGCTGC CCCTCCGCCT GGGCACTGTA GAAGGCTGTG TTGTGGCTGC TTATCACTTC 
TCCCAAGTCC TCCGCAGAAG CCGCCTTCAG GGAGATTGCT ACTATGACCA ACCAGATGAA 
GACAGCCCTC TCGGCTCACG TAGCTCCCAC CAGATGGTTG AGGAGCTTAT GATCCTATTC 
AATAGCTATG TAGCTGAATT CCTGGTGCAC CAGGATAACA CAAAGCTGAT CACCCCATTG 
AGGTGCCAGG CTGAGCCCAA TCCTCAGCAA GTAGCTCAGT TAAAGGATAA ATACAGTCAC 
TTGCTCCCTC TGTCCCTTCA TCTCTCTTAT CGTCTCTGTG GCCAGACCAC CAGCACTTTC 
CAAGGAACCA GTTCAGGATA CAACCTTTGC ATCCTGCGCC CGCTTTGGGA CCACCTGTGC 
CAAGCTGCTG ACAGCAAGGA CTATAACAAG ATGGTGGACT TAATTGCCAC AGATGACATC 
CACCCCACTC TGGCCCCAGC TGGACTGGAG TTCCGAAAGC TGCTCAGCCG GTCCTACTTT 
AGCCGCTCCA ACTCCACCAG CCTGTCCAGG GCTGGGCATT ACTCGTTGCA GGTGGACTGG 
TATACTTGGG CATCATCCCC CATAAGGCGC TATATTGATG TGGTGCTCCA GCGGCAGCTC 
CATGCTGCCA TGACCAAGGG CCATCCTGGA TATTCCCCTG AAGATATTGA CTTACTGTGC 
CATGACTTCA ACCGGAAGAA TGGACGCGCA CAGGCTTACG AGCGCAAGGC CCACTGCCTT 
CACCTTGCCA CCCAGCTCAA GACCCAGCTG CTGACAAAGC TGTGCTTTGT GGTAAATGTG 
GAATCTGCAA ACCGCTTTTT CAAGGTGATT TTCCCCATGA ACCGGGATAC CCTGCCTGAT 
CCCCACCTTG TCAATTATCG TGCACTGCAA CTGGTAGAGC AGCCCACCTT CATCCCTGAG 
CACCAGAAAA TGAAGTTGGT TTGGAAGAGG CGAATATACT CTCTGGATAA GGGCAAAGCA 
TGGATGCCAT CCTCTAAAAC CCTGGTAGAC ACCCATGTCA TGCCAGTAAG CAGTGTCATC 
TGGCAGGACC TGCTGGTTGC AATCCAGAGT CAGAATTTTC AGAAAGCAGC AGCCTTGCTC 
CTAAGGTGTC GAGGGACTGT TCAGACCTCC CACCATCGGT CCTTAGGCCA GGTGTCTCGA 
AGCAAGTGCT CGCACTATGT AGAGGTGATA GTAGAGCTAA GTACAGGGGA TGCCCTGCAG 
ATGCAACTGA CTACTGATGT CCAGAGGGGC TTCCTTGTGC CCTCCATTCA GATGTGGATG 
TTGACCCCAG GTTTTGCCAT TTGCCTGGAG CATGCTGAGA AACCCATTGA CTGCTTTTCC 
CGCTATGCTT CACAGACAAC GAAAGATGTG TATTCTGAGG CACAGGAATA TTCCCGGGTA 
TGGGAACCAC TCTGTGCCAT GGAGTCCGCC TCCAGTGCTG TGGCTGAGAA CGACTCTATT 
CTCTTGCATG GTGTTCCCAT TACTTGGGAC CAAGAGCGGA ATGACCAGGG ACAGATGCAA 
GGTTCCTTCT GCTTAACCTA TAGCTTCCTC AAGGAATGTG CGATTGACAT CAACTTTAAT 
TTCTGCTACC TCTGCATCCG GTTGGAGAAC CTGAAACTCC AGGGTCCAGT AGAAGGGGAG 
GAGGAGAGGG AAAAAGGAAA GGAGGCTGCT CTGTGTCAAG GCTTTAGGAA GCTCAGCTTG 
GGCCACACGG CAGCAAACCC ATCTGTGGTC AATACTCTTG ACTCTTGTTT GAATGTAGAC 
CCTGACACGT ATACCTGGGT GGCTCATGGG CTGACAGATG AAATGGATCA GGATTCCAAA 
AAAGTTGATC GAAGAGAAGA ACGACAGAGA ATCCATTTTT TCCTACATCA TGTAGCCATG 
GAGAGCATTC CCGTGGAGGT CACCAGAAAG GAAGCTTGCT TCACCATTGA GATGATCCCA 
AAGCTTCTGC CAGATATCCG GAAAGAGGAA GCCATCTGGA AACTGAAATA TGCCTCACCA 
CTTGTCACCA GCATTGCCTT AGGCCAGCCC ATTAAGGAAG TGTCCTGCGT AGCCATGAAG 
TCCAGTATCC TGAAGCGCCA GACCTTTGAC ATTCCCCGAG GAAAATTCAA GCTCAATAAG 
AGTCAGAACA GTGCTGTGCT AGAAGCTCTT CAAAAGCCTT TTACCCTCAT CCAAGGGCCA 
CCAGGCACTG GGAAGACGAT GGTCGGGGTC CATATTGTAT ACTGGTTCCA TAAGCTAAAC 
CAGGAGAAGC CTGAAACTGC TCCGCTGCCC TTGAGAGGGG AGAAGAAAGA CAAAGGGGAG 
AAGTGCATCC TATATTGTGG GCCATCCAAC AAATCTGTTG ATGTTGTTTC AGAAATCCTC 
CTTCAGTGGA AGGAGGAACT CAGACCATTG CAAGTATATA GTGAACAGAT GGAGGCCATG 
GAATTCCCTG TACCTGGGAG CTATAGAAAT ATGTTTCGCA AAAATCTCCG GGAAGGGAAG 
ACAAAACCTG AGCTCAGACA GATCATCCTT CATCATCGTA TCCGGATGCC GTCCAATCCA 
TATGCTCGCA AGATTATGGA ATTTGATACC AGAGTGAAGA TGGGGGATCA GTTTTCAGAG 
GAAGAGATTG AACTATATAA GTCCATCACC ACGACGGCCC GGAAATATGA ACTAAGCAAC 
CATGACATAA TCCTCTGTAC TTGCTCCTGT TCAGCCTCCT CCATTCTCAC CAAACAACTC 
AATGTCAAAC AAATTCTCAT TGATGAAGCT GGCATGTCCA CAGAGCCTGA AAGCCTCATC 
CCCCTTGTTA ACTTTCCCCG GGCTGAGAAG GTGGTGTTGC TAGGGGACCA CAAACAGCTT 
CAGCCTTTTG TAAAGAATGA TCACTGTCAA AACCTGGGCT TGGAGAAGTC CCTCTTCGAG 
CGTTACCATC GTCAAGCCTG GATGCTGGAC ACACAGTATC GAATGCACAA GGACATCTGC 
AAATTCCCTT CCAAAGAATT TTATGACAAT AAATTAAAGA CTAGCATGGA ACTAAGGCGA 
ACGCCAAGTG TCTTCAGCCA CCAAGATCAA GAAAGCTGTC CCATCATCTT TGGCTATGTC 
CAGGGAGAAG AGAAGAGTCT GGTAGTCTCC ACAGATGAAG GAAATGAAAA CTCCAAAGCT 
AACCAAGATG AAGTGGAGCA GGTGATTCGA ATAGCAAAGC AGCTGACCTT GGATAGGACC 
ATCAAACCCA AGGACATTGC AATCCTCAGC CCATACAATG CCCAGGTATC AGAGATCAAC 
AAGAGACTAT TAAAGGAGGG AATTACTGGA GTGACTGTCT CCTCCATCAA GAAGAGCCAA 
GGAAGTGAGT GGCGATATGT CATTCTAAGC ACAGTCCGCT CCTGCCCTCT TAGCGAAATT 
GATGGGAAGC CCACCAAAAG CTGGCTGAAG AAACACCTGG GTTTTGTCAG TGACCCACAT 
CAGGTCAATG TGGGGATCAC TCGGGCCCAA GAGGGGCTCT GCATCATTGG AAATCACTAC 
CTCTTGAGTT GCTGTCCAAT GTGGCATCGG CTCATTGATT TCTACACCAA GAAGGGATGC 
TGTGTCATGG CTTCCTCAGT GCAAGTGCGT CAGAGGCCAG CTATCTACCA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HELZ2 ( XM_012554098.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012409552.1 Sarcophilus harrisii helicase with zinc finger 2, transcriptional coactivator (HELZ2), mRNA.

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CloneID	OSa119203
Clone ID Related Accession (Same CDS sequence)	XM_012554098.2 , XM_012554098.3 , XM_012554098.1
Accession Version	XM_012554098.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	helicase with zinc finger domain 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816669.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCAC TCAAAAAGGG GCTTCTGGCT CGGCTCCAGA GCCAACTGGA TCAGCTTCAG 
AGCCAACTGG ACCTACGTGT GGGCTGCTCA GTCTGCTCAG AGCCATTGAA TGAGAGCACA 
TATAAATGGC ATACCATAGA ACACAACTGC CCCCGTGAGA TTCTCCTGGC AAGGGCCAAG 
GGAGCTCCTG AGCATAAAGA GTGGAGAAAG GTTGGACGCA GGCCCAGCTT CCCCCGGCCC 
AGACGTTACG AAGTATGCCG CTACTACAAA CCTGGGATGG GCTGCAGGAA ACACCGCAAC 
CAGTGCACAT TTGCCCGAAG CTTAGAGGAA GCATTGGTTT GGACTTTTGA GAGGGAAAAC 
AATATCCCCA GACTGTGGCT GAAAGCTAAA GTACAGGGAA GCTTGTATCA AGGGACTCCC 
AGGATTCTGA CTGCAGCTGA AGAAATAGGG GCTGAATTTG GAGGCTACTT TCAGGAACTT 
TGTAAGCCTT GCTTCCGGAA CTGCCCTGCA CAGGTGACTC TCCGGGGCCC AAACCTGCCA 
TGCCCACAGC ATGGGCCCTC TCCCCTGCTT ATCGTACACA TAAGTGAGGG TTTTAGAAAA 
AAGCAGCTAG TGGAGATCCG GCCCCGGCCC CGGCAAGGCC ACCTACTGGA ATATTGTATG 
TACGTGTCTC GAGGACATCC GTGTCGGCAC GGAGCTGTCC GCTGCCAATA TGCTCACAGT 
GATGTGGAGA TGGCTGTGTG GGAAGCAGAA TCAATGGGCT GCCTTACTCG CTCTGACTTG 
CTTGTGCTAC CAGCCTCACT GGAATCTGGA GCAGGCTCTG GGACCAGCGG TGAGGTTGGT 
GGTAGAAATG GGAGCCCATC TGCTCCACCC CAGATTCAGC TGTATTGCCG TGTCTGTCTT 
GTAACTTGCA GCTCCCAAGA GAGTTTTGAG AACCATTGCT CATCCCTGGA ACACACACAG 
ATGTTGTCCT ATGACCATGG GGCCCTATGG GAATACCGGG CCCCCCCCAT TGGGCTCCGC 
AAATTCAAAC TTTGCAGCAG GCTAGATATT TGTGAATATG GGAATGGCTG CACTCAGGCA 
CACTCCCAGG AAGAGCTTCA AGAGTGGATT ATGCGGGCTC AGGCAGCCCA GATGAGAGAA 
GAATCGGCCC GGCAGGATGG ACTCCTCTCC TACCAGGACC GGCTTTTACA TGAATATCAA 
CACTGCCACA ATGAAATACA GGTGATGTCC GAGTCTATAC GTGATGTAAC TATTCACTGT 
GACCAGCCCT TAAAATGCCA GGTAGAGGAC AAGAAATCGC AATGCATATG GAAATTTATC 
ATCCATTCTA AGGACCCACT GCAGCACGTG GCCCTCCTCA AACATCAGAT GGGAGCCATC 
TTTTCCTTGG TGTGTCCTGG GCTTCCACAA AGACAGCTTT ATGCCCAAGG CTCAAGGTTC 
CATGTGCAGG ATACAGCGAT GACCTTCCAG GTGGAGGTGC TGGTAGAGTG TATCACCTTT 
GGCAGCTATG AACAATGGGT AGCTTTTGAC TTTGGACGCC GACCTGTCCT GGTACAGAAG 
CTATATGTTC AAGTGGGCCA AAAGGAGACC CTTGGGCCTC CCCCCTGTAT CCAGGACCAT 
AATTGTCTTC TTGAGTTGAT CCGCTGGCAC AGTGGAAATC GATGTGTGGT GCCCAATGTG 
GGCAGGACAG CTGAGCAGGT GAATCTGCTA GCTAAGTACA AAGTGAGCCC CCAGGAACTG 
GAGAACTACA GGTCAAAGGC TTCAGACCAG GTCTCTATAA CTCCAGTCAA CTATCGGGAG 
AGGATGCACC AATTCTTGTA TATGGAAGAG GAGGCTCAAC AGGAGCTGGT GGCCAAATTG 
AACCTTCAAG TCGAAGTCAC ATTGACCTCC AAGATGCAGA CCTTGGCAAT GGGCATGAAG 
TTTGCACCAT CTGGGGAATT GTATGCTAAG GTTCCAATTC CTTGTTCCCT GACACCAGAC 
ACAGACCAGG GCTATTTGCT AAGTAGAGCT GTTAGCACTG CTTACGTGGC ACCTGTCCCT 
GCCCCTGACA ATCATGTGTA TGAGGTTCGG GTGGAAGTTA AAGCCACTTC AGAGCAGAAT 
GTGTGGCTCC TACTGCCAGC CCACTGCTGC TCTGCCTTGG GACTTAAGGA TGAAGACACA 
CAGCTTATGG AAATCCAGTT TCAAATCGAC AAGACTATAT TTGGGATATG GCACCAAGCC 
ATTGATGCAC TTCAGGATGA TAGACTGGTG GCACCTGATG TACCTGCCTG TTCTCTGCCT 
TCTTTGAGGC CCCCTCCCCA GGAACTCCGT GGTAATCCCA AGCAGAAGGA GGCCATCTCC 
TTTATCACAG GGACAACGAG TGGTCAGAGC CGAGTGCCTC CACTGCTCAT TTATGGCCCC 
TTTGGGACAG GCAAGACATA CACACTAGCT ATGGCCACGC TAGAGGTTAT CAAACAGCCA 
AACACAAAAG TGTTGATCTG TACTCACACC AACAGTGCTG CTGACATCTA CATCCGTGAG 
TACTTTCATG CTTATGTCAC AGCTGGACAC CCTGAGGCTG TGCCATTGAG GGTCAAAAAC 
ACTGAAAGCT TCCTCAACCA GACAGACCCG ACCACTCTGC AGTATTGCTG CCTCTCAAGT 
GATGGACGGT CCTTTTGCTT CCCAACTCAG GAGAAACTAA AGCAGCACCG CATTATAATC 
ACTACCACTA CCTTGTCCCG CTCCCTATGT GTGCCATCTG GGTTTTTTTC CCACATTCTC 
ATTGATGAGG CTGCTCAGAT GTTAGAATGT GAAGCCATCA TCCCACTGGC CTATGCTACC 
CAGAAGACAC GCATTGTCCT TGCCGGGGAC CACATGCAAA TCACCCCCAA ACTCTTCAGT 
GTTGGGGCCA GGAAGTCTGC TGACCATACA CTGCTTAATC GGCTGTTCCA GTACTATCAG 
AAGGAGACTC ATAAATTGGC TTCCCAGAGC AGGGTCATCT TCCATGAAAA CTACCGTTCC 
ACCAAAGCCA TCATTTCCTT TGTGTCTCGG AATTTCTATG TGGCTAAGGG TAACCCTATT 
CAGGCTAGCG GACAGATCCC TGCACACCCT TGCAAGTACC CACTCATGTT TTGCCATGTG 
GCTGGTACTC CTGAGAGAGA CATAACAATG ACATCATGGT TTAACAGGGC TGAGATAGTG 
GAGGTGATTG AGAAGGTGCA AGAATTCTAT CGTATCTGGG CCTACCAATG GAATAGCCCT 
GACCTAAAGC AAATTTGTGT TGTGTCCCAT GGTTCTCAGG TAAAAATATT GAGGCAGGAA 
CTGAGGAAGA AACAACTAGG AGAAGTAGCA GTGGAGAATT TTGAAAATCT GCCAGGTCGT 
GAATTCCGAG TGATCATCCT CAGCACAGTC CACACTCGGG ACAGCCTGCT CAACAGCTCA 
GTCCCCCACC TAGAATTCTT CAGTGAGGCT CGGGTGCTGA ACACCGTGAT GACCAGAGCC 
CAGTCCCAGG TGGTTGCTGT GGGCGATGCT GTGGCCCTTT GTTCTTTTGG ACGTTGTAGC 
AAGGTATGGA AGCACTTCAT CCAAGAGTGT ATAGAGATGC GCAGTGTGTC CCCTGTGGAC 
CTCACAATGG AACAAATCAA GCAAGATGTG GCACACAGGC AGCGCTGGGC CCGGCAAAAT 
GGACTGCATC CTATGAACTC TCCCAGGGAG CACTCGTTAG AAGAGGAAGA CAATGACAAT 
GACATTCCCT CATGGGTGTC TGATGCTGAG CTCAATGCTG ATGATCCCAT CCTCCAGGAG 
TTGCTGGATG AGAGTAAGAA TGTGGCAGTG ACAGTAAGTG AGGATGGGCT GCTGAATGTG 
ACATCAGGGT CCCGGTCCCG CTATCAGGGA CAACAGTATT TAAGCTTTCC TGTGCCAGTG 
TTGCAGAAAT ATCTCCGAAT GCAACCCAAG ATATATAGGA AATGTGAACT GATAAAGGAG 
GCCTTTGAGA AGGCATCTGC CTTCACCATA GATGACTCCC CACCCCTCAT TATTCAGATC 
AGAGGACGCA CCAACTGTGG AATGGCCTTC ACTGGGGATC AAGTTCTAGT GGAAATCCTG 
ACTCCAGAAT TGGAGGAACA GGGGTACTCC TGGCGCCCCA TGGGCCGAGT GGTAGGAATA 
CTAGAAAGAG GTGACCGTGA ACTCATCTTC ATTTGCAAAA TGGATGAGTT TGACCCCCGA 
GTTATGATCC CCATTGACCC GTCAGTCACC AAGATCTTTG TGCCAGAGTT AAAGGATAAG 
CCCAACACGA TTCCTATCCG CCGCCTACAA CGAGGACACA TCAAGCTTAT TAGCTATGAG 
AAGATAACAG AGGAGACCAA GCGTAATCAG CTCTTTCGTG TTCAGATCAT CTCTTGGAAA 
GAGAGGTTCT ATTATCCCTT AGGCATCATT CTTGAGATTC TTCCCTTGCC TTTGACCTTA 
GAACAAGGTT TAAAGATTCT GGACATGGAA TATTCTCTGA ATGCTAATGG AAGATACCCA 
ACTCCAGTAA TTAAGGAAGT GAACCAACAC AACTGTAGTA AATTGAATCT GCACCGGGGA 
CAGTGGAAGG ACTGCCGGGA TTATTTGACC TTTACTGTGG ACCCGCAGGG CTCCCGGGAT 
CTGGATGATG CTATCAGTGT CCGGGACCTG GGAAGCCATT ATGAAATTGC TGTCCATATA 
TCTGATGTGG CTAGCTTCAT GCCAAAGGAT GGTGCACTTG ACCGAGAGGC TAGAAAGCGA 
GGTGCTACCT ACTATGCTCC TGGAAAGGAT CCAGTGGCCA TGCTCCCTTC CAGGCTCAGC 
CAGGACCTGT TCAGTCTCCT TCCCCAGCAA GAACGTCGCA CTATCTCCCT GATTCTTACA 
GTGGAGAAGG AAAGTGACCG AGTAGTGCAA TGGCATATCA CACCATCTAT GATATGCTCT 
GACCTCCAAC TGACGTATGA GGAGGCTGAG GTTATTATTA AGAGTCATCC TGGGGGTGGG 
GCCTGGCTGC CCCTCCGCCT GGGCACTGTA GAAGGCTGTG TTGTGGCTGC TTATCACTTC 
TCCCAAGTCC TCCGCAGAAG CCGCCTTCAG GGAGATTGCT ACTATGACCA ACCAGATGAA 
GACAGCCCTC TCGGCTCACG TAGCTCCCAC CAGATGGTTG AGGAGCTTAT GATCCTATTC 
AATAGCTATG TAGCTGAATT CCTGGTGCAC CAGGATAACA CAAAGCTGAT CACCCCATTG 
AGGTGCCAGG CTGAGCCCAA TCCTCAGCAA GTAGCTCAGT TAAAGGATAA ATACAGTCAC 
TTGCTCCCTC TGTCCCTTCA TCTCTCTTAT CGTCTCTGTG GCCAGACCAC CAGCACTTTC 
CAAGGAACCA GTTCAGGATA CAACCTTTGC ATCCTGCGCC CGCTTTGGGA CCACCTGTGC 
CAAGCTGCTG ACAGCAAGGA CTATAACAAG ATGGTGGACT TAATTGCCAC AGATGACATC 
CACCCCACTC TGGCCCCAGC TGGACTGGAG TTCCGAAAGC TGCTCAGCCG GTCCTACTTT 
AGCCGCTCCA ACTCCACCAG CCTGTCCAGG GCTGGGCATT ACTCGTTGCA GGTGGACTGG 
TATACTTGGG CATCATCCCC CATAAGGCGC TATATTGATG TGGTGCTCCA GCGGCAGCTC 
CATGCTGCCA TGACCAAGGG CCATCCTGGA TATTCCCCTG AAGATATTGA CTTACTGTGC 
CATGACTTCA ACCGGAAGAA TGGACGCGCA CAGGCTTACG AGCGCAAGGC CCACTGCCTT 
CACCTTGCCA CCCAGCTCAA GACCCAGCTG CTGACAAAGC TGTGCTTTGT GGTAAATGTG 
GAATCTGCAA ACCGCTTTTT CAAGGTGATT TTCCCCATGA ACCGGGATAC CCTGCCTGAT 
CCCCACCTTG TCAATTATCG TGCACTGCAA CTGGTAGAGC AGCCCACCTT CATCCCTGAG 
CACCAGAAAA TGAAGTTGGT TTGGAAGAGG CGAATATACT CTCTGGATAA GGGCAAAGCA 
TGGATGCCAT CCTCTAAAAC CCTGGTAGAC ACCCATGTCA TGCCAGTAAG CAGTGTCATC 
TGGCAGGACC TGCTGGTTGC AATCCAGAGT CAGAATTTTC AGAAAGCAGC AGCCTTGCTC 
CTAAGGTGTC GAGGGACTGT TCAGACCTCC CACCATCGGT CCTTAGGCCA GGTGTCTCGA 
AGCAAGTGCT CGCACTATGT AGAGGTGATA GTAGAGCTAA GTACAGGGGA TGCCCTGCAG 
ATGCAACTGA CTACTGATGT CCAGAGGGGC TTCCTTGTGC CCTCCATTCA GATGTGGATG 
TTGACCCCAG GTTTTGCCAT TTGCCTGGAG CATGCTGAGA AACCCATTGA CTGCTTTTCC 
CGCTATGCTT CACAGACAAC GAAAGATGTG TATTCTGAGG CACAGGAATA TTCCCGGGTA 
TGGGAACCAC TCTGTGCCAT GGAGTCCGCC TCCAGTGCTG TGGCTGAGAA CGACTCTATT 
CTCTTGCATG GTGTTCCCAT TACTTGGGAC CAAGAGCGGA ATGACCAGGG ACAGATGCAA 
GGTTCCTTCT GCTTAACCTA TAGCTTCCTC AAGGAATGTG CGATTGACAT CAACTTTAAT 
TTCTGCTACC TCTGCATCCG GTTGGAGAAC CTGAAACTCC AGGGTCCAGT AGAAGGGGAG 
GAGGAGAGGG AAAAAGGAAA GGAGGCTGCT CTGTGTCAAG GCTTTAGGAA GCTCAGCTTG 
GGCCACACGG CAGCAAACCC ATCTGTGGTC AATACTCTTG ACTCTTGTTT GAATGTAGAC 
CCTGACACGT ATACCTGGGT GGCTCATGGG CTGACAGATG AAATGGATCA GGATTCCAAA 
AAAGTTGATC GAAGAGAAGA ACGACAGAGA ATCCATTTTT TCCTACATCA TGTAGCCATG 
GAGAGCATTC CCGTGGAGGT CACCAGAAAG GAAGCTTGCT TCACCATTGA GATGATCCCA 
AAGCTTCTGC CAGATATCCG GAAAGAGGAA GCCATCTGGA AACTGAAATA TGCCTCACCA 
CTTGTCACCA GCATTGCCTT AGGCCAGCCC ATTAAGGAAG TGTCCTGCGT AGCCATGAAG 
TCCAGTATCC TGAAGCGCCA GACCTTTGAC ATTCCCCGAG GAAAATTCAA GCTCAATAAG 
AGTCAGAACA GTGCTGTGCT AGAAGCTCTT CAAAAGCCTT TTACCCTCAT CCAAGGGCCA 
CCAGGCACTG GGAAGACGAT GGTCGGGGTC CATATTGTAT ACTGGTTCCA TAAGCTAAAC 
CAGGAGAAGC CTGAAACTGC TCCGCTGCCC TTGAGAGGGG AGAAGAAAGA CAAAGGGGAG 
AAGTGCATCC TATATTGTGG GCCATCCAAC AAATCTGTTG ATGTTGTTTC AGAAATCCTC 
CTTCAGTGGA AGGAGGAACT CAGACCATTG CAAGTATATA GTGAACAGAT GGAGGCCATG 
GAATTCCCTG TACCTGGGAG CTATAGAAAT ATGTTTCGCA AAAATCTCCG GGAAGGGAAG 
ACAAAACCTG AGCTCAGACA GATCATCCTT CATCATCGTA TCCGGATGCC GTCCAATCCA 
TATGCTCGCA AGATTATGGA ATTTGATACC AGAGTGAAGA TGGGGGATCA GTTTTCAGAG 
GAAGAGATTG AACTATATAA GTCCATCACC ACGACGGCCC GGAAATATGA ACTAAGCAAC 
CATGACATAA TCCTCTGTAC TTGCTCCTGT TCAGCCTCCT CCATTCTCAC CAAACAACTC 
AATGTCAAAC AAATTCTCAT TGATGAAGCT GGCATGTCCA CAGAGCCTGA AAGCCTCATC 
CCCCTTGTTA ACTTTCCCCG GGCTGAGAAG GTGGTGTTGC TAGGGGACCA CAAACAGCTT 
CAGCCTTTTG TAAAGAATGA TCACTGTCAA AACCTGGGCT TGGAGAAGTC CCTCTTCGAG 
CGTTACCATC GTCAAGCCTG GATGCTGGAC ACACAGTATC GAATGCACAA GGACATCTGC 
AAATTCCCTT CCAAAGAATT TTATGACAAT AAATTAAAGA CTAGCATGGA ACTAAGGCGA 
ACGCCAAGTG TCTTCAGCCA CCAAGATCAA GAAAGCTGTC CCATCATCTT TGGCTATGTC 
CAGGGAGAAG AGAAGAGTCT GGTAGTCTCC ACAGATGAAG GAAATGAAAA CTCCAAAGCT 
AACCAAGATG AAGTGGAGCA GGTGATTCGA ATAGCAAAGC AGCTGACCTT GGATAGGACC 
ATCAAACCCA AGGACATTGC AATCCTCAGC CCATACAATG CCCAGGTATC AGAGATCAAC 
AAGAGACTAT TAAAGGAGGG AATTACTGGA GTGACTGTCT CCTCCATCAA GAAGAGCCAA 
GGAAGTGAGT GGCGATATGT CATTCTAAGC ACAGTCCGCT CCTGCCCTCT TAGCGAAATT 
GATGGGAAGC CCACCAAAAG CTGGCTGAAG AAACACCTGG GTTTTGTCAG TGACCCACAT 
CAGGTCAATG TGGGGATCAC TCGGGCCCAA GAGGGGCTCT GCATCATTGG AAATCACTAC 
CTCTTGAGTT GCTGTCCAAT GTGGCATCGG CTCATTGATT TCTACACCAA GAAGGGATGC 
TGTGTCATGG CTTCCTCAGT GCAAGTGCGT CAGAGGCCAG CTATCTACCA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012409552.1
CDS	328..8961
Translation	MPSLKKGLLARLQSQLDQLQSQLDLRVGCSVCSEPLNESTYKWHTIEHNCPREILLARAKGAPEHKEWRKVGRRPSFPRPRRYEVCRYYKPGMGCRKHRNQCTFARSLEEALVWTFERENNIPRLWLKAKVQGSLYQGTPRILTAAEEIGAEFGGYFQELCKPCFRNCPAQVTLRGPNLPCPQHGPSPLLIVHISEGFRKKQLVEIRPRPRQGHLLEYCMYVSRGHPCRHGAVRCQYAHSDVEMAVWEAESMGCLTRSDLLVLPASLESGAGSGTSGEVGGRNGSPSAPPQIQLYCRVCLVTCSSQESFENHCSSLEHTQMLSYDHGALWEYRAPPIGLRKFKLCSRLDICEYGNGCTQAHSQEELQEWIMRAQAAQMREESARQDGLLSYQDRLLHEYQHCHNEIQVMSESIRDVTIHCDQPLKCQVEDKKSQCIWKFIIHSKDPLQHVALLKHQMGAIFSLVCPGLPQRQLYAQGSRFHVQDTAMTFQVEVLVECITFGSYEQWVAFDFGRRPVLVQKLYVQVGQKETLGPPPCIQDHNCLLELIRWHSGNRCVVPNVGRTAEQVNLLAKYKVSPQELENYRSKASDQVSITPVNYRERMHQFLYMEEEAQQELVAKLNLQVEVTLTSKMQTLAMGMKFAPSGELYAKVPIPCSLTPDTDQGYLLSRAVSTAYVAPVPAPDNHVYEVRVEVKATSEQNVWLLLPAHCCSALGLKDEDTQLMEIQFQIDKTIFGIWHQAIDALQDDRLVAPDVPACSLPSLRPPPQELRGNPKQKEAISFITGTTSGQSRVPPLLIYGPFGTGKTYTLAMATLEVIKQPNTKVLICTHTNSAADIYIREYFHAYVTAGHPEAVPLRVKNTESFLNQTDPTTLQYCCLSSDGRSFCFPTQEKLKQHRIIITTTTLSRSLCVPSGFFSHILIDEAAQMLECEAIIPLAYATQKTRIVLAGDHMQITPKLFSVGARKSADHTLLNRLFQYYQKETHKLASQSRVIFHENYRSTKAIISFVSRNFYVAKGNPIQASGQIPAHPCKYPLMFCHVAGTPERDITMTSWFNRAEIVEVIEKVQEFYRIWAYQWNSPDLKQICVVSHGSQVKILRQELRKKQLGEVAVENFENLPGREFRVIILSTVHTRDSLLNSSVPHLEFFSEARVLNTVMTRAQSQVVAVGDAVALCSFGRCSKVWKHFIQECIEMRSVSPVDLTMEQIKQDVAHRQRWARQNGLHPMNSPREHSLEEEDNDNDIPSWVSDAELNADDPILQELLDESKNVAVTVSEDGLLNVTSGSRSRYQGQQYLSFPVPVLQKYLRMQPKIYRKCELIKEAFEKASAFTIDDSPPLIIQIRGRTNCGMAFTGDQVLVEILTPELEEQGYSWRPMGRVVGILERGDRELIFICKMDEFDPRVMIPIDPSVTKIFVPELKDKPNTIPIRRLQRGHIKLISYEKITEETKRNQLFRVQIISWKERFYYPLGIILEILPLPLTLEQGLKILDMEYSLNANGRYPTPVIKEVNQHNCSKLNLHRGQWKDCRDYLTFTVDPQGSRDLDDAISVRDLGSHYEIAVHISDVASFMPKDGALDREARKRGATYYAPGKDPVAMLPSRLSQDLFSLLPQQERRTISLILTVEKESDRVVQWHITPSMICSDLQLTYEEAEVIIKSHPGGGAWLPLRLGTVEGCVVAAYHFSQVLRRSRLQGDCYYDQPDEDSPLGSRSSHQMVEELMILFNSYVAEFLVHQDNTKLITPLRCQAEPNPQQVAQLKDKYSHLLPLSLHLSYRLCGQTTSTFQGTSSGYNLCILRPLWDHLCQAADSKDYNKMVDLIATDDIHPTLAPAGLEFRKLLSRSYFSRSNSTSLSRAGHYSLQVDWYTWASSPIRRYIDVVLQRQLHAAMTKGHPGYSPEDIDLLCHDFNRKNGRAQAYERKAHCLHLATQLKTQLLTKLCFVVNVESANRFFKVIFPMNRDTLPDPHLVNYRALQLVEQPTFIPEHQKMKLVWKRRIYSLDKGKAWMPSSKTLVDTHVMPVSSVIWQDLLVAIQSQNFQKAAALLLRCRGTVQTSHHRSLGQVSRSKCSHYVEVIVELSTGDALQMQLTTDVQRGFLVPSIQMWMLTPGFAICLEHAEKPIDCFSRYASQTTKDVYSEAQEYSRVWEPLCAMESASSAVAENDSILLHGVPITWDQERNDQGQMQGSFCLTYSFLKECAIDINFNFCYLCIRLENLKLQGPVEGEEEREKGKEAALCQGFRKLSLGHTAANPSVVNTLDSCLNVDPDTYTWVAHGLTDEMDQDSKKVDRREERQRIHFFLHHVAMESIPVEVTRKEACFTIEMIPKLLPDIRKEEAIWKLKYASPLVTSIALGQPIKEVSCVAMKSSILKRQTFDIPRGKFKLNKSQNSAVLEALQKPFTLIQGPPGTGKTMVGVHIVYWFHKLNQEKPETAPLPLRGEKKDKGEKCILYCGPSNKSVDVVSEILLQWKEELRPLQVYSEQMEAMEFPVPGSYRNMFRKNLREGKTKPELRQIILHHRIRMPSNPYARKIMEFDTRVKMGDQFSEEEIELYKSITTTARKYELSNHDIILCTCSCSASSILTKQLNVKQILIDEAGMSTEPESLIPLVNFPRAEKVVLLGDHKQLQPFVKNDHCQNLGLEKSLFERYHRQAWMLDTQYRMHKDICKFPSKEFYDNKLKTSMELRRTPSVFSHQDQESCPIIFGYVQGEEKSLVVSTDEGNENSKANQDEVEQVIRIAKQLTLDRTIKPKDIAILSPYNAQVSEINKRLLKEGITGVTVSSIKKSQGSEWRYVILSTVRSCPLSEIDGKPTKSWLKKHLGFVSDPHQVNVGITRAQEGLCIIGNHYLLSCCPMWHRLIDFYTKKGCCVMASSVQVRQRPAIYH

Target ORF information:

RefSeq Version	XM_012554098.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii helicase with zinc finger 2, transcriptional coactivator (HELZ2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012554098.1

ORF Insert Sequence:

ATGCCGTCAC TCAAAAAGGG GCTTCTGGCT CGGCTCCAGA GCCAACTGGA TCAGCTTCAG 
AGCCAACTGG ACCTACGTGT GGGCTGCTCA GTCTGCTCAG AGCCATTGAA TGAGAGCACA 
TATAAATGGC ATACCATAGA ACACAACTGC CCCCGTGAGA TTCTCCTGGC AAGGGCCAAG 
GGAGCTCCTG AGCATAAAGA GTGGAGAAAG GTTGGACGCA GGCCCAGCTT CCCCCGGCCC 
AGACGTTACG AAGTATGCCG CTACTACAAA CCTGGGATGG GCTGCAGGAA ACACCGCAAC 
CAGTGCACAT TTGCCCGAAG CTTAGAGGAA GCATTGGTTT GGACTTTTGA GAGGGAAAAC 
AATATCCCCA GACTGTGGCT GAAAGCTAAA GTACAGGGAA GCTTGTATCA AGGGACTCCC 
AGGATTCTGA CTGCAGCTGA AGAAATAGGG GCTGAATTTG GAGGCTACTT TCAGGAACTT 
TGTAAGCCTT GCTTCCGGAA CTGCCCTGCA CAGGTGACTC TCCGGGGCCC AAACCTGCCA 
TGCCCACAGC ATGGGCCCTC TCCCCTGCTT ATCGTACACA TAAGTGAGGG TTTTAGAAAA 
AAGCAGCTAG TGGAGATCCG GCCCCGGCCC CGGCAAGGCC ACCTACTGGA ATATTGTATG 
TACGTGTCTC GAGGACATCC GTGTCGGCAC GGAGCTGTCC GCTGCCAATA TGCTCACAGT 
GATGTGGAGA TGGCTGTGTG GGAAGCAGAA TCAATGGGCT GCCTTACTCG CTCTGACTTG 
CTTGTGCTAC CAGCCTCACT GGAATCTGGA GCAGGCTCTG GGACCAGCGG TGAGGTTGGT 
GGTAGAAATG GGAGCCCATC TGCTCCACCC CAGATTCAGC TGTATTGCCG TGTCTGTCTT 
GTAACTTGCA GCTCCCAAGA GAGTTTTGAG AACCATTGCT CATCCCTGGA ACACACACAG 
ATGTTGTCCT ATGACCATGG GGCCCTATGG GAATACCGGG CCCCCCCCAT TGGGCTCCGC 
AAATTCAAAC TTTGCAGCAG GCTAGATATT TGTGAATATG GGAATGGCTG CACTCAGGCA 
CACTCCCAGG AAGAGCTTCA AGAGTGGATT ATGCGGGCTC AGGCAGCCCA GATGAGAGAA 
GAATCGGCCC GGCAGGATGG ACTCCTCTCC TACCAGGACC GGCTTTTACA TGAATATCAA 
CACTGCCACA ATGAAATACA GGTGATGTCC GAGTCTATAC GTGATGTAAC TATTCACTGT 
GACCAGCCCT TAAAATGCCA GGTAGAGGAC AAGAAATCGC AATGCATATG GAAATTTATC 
ATCCATTCTA AGGACCCACT GCAGCACGTG GCCCTCCTCA AACATCAGAT GGGAGCCATC 
TTTTCCTTGG TGTGTCCTGG GCTTCCACAA AGACAGCTTT ATGCCCAAGG CTCAAGGTTC 
CATGTGCAGG ATACAGCGAT GACCTTCCAG GTGGAGGTGC TGGTAGAGTG TATCACCTTT 
GGCAGCTATG AACAATGGGT AGCTTTTGAC TTTGGACGCC GACCTGTCCT GGTACAGAAG 
CTATATGTTC AAGTGGGCCA AAAGGAGACC CTTGGGCCTC CCCCCTGTAT CCAGGACCAT 
AATTGTCTTC TTGAGTTGAT CCGCTGGCAC AGTGGAAATC GATGTGTGGT GCCCAATGTG 
GGCAGGACAG CTGAGCAGGT GAATCTGCTA GCTAAGTACA AAGTGAGCCC CCAGGAACTG 
GAGAACTACA GGTCAAAGGC TTCAGACCAG GTCTCTATAA CTCCAGTCAA CTATCGGGAG 
AGGATGCACC AATTCTTGTA TATGGAAGAG GAGGCTCAAC AGGAGCTGGT GGCCAAATTG 
AACCTTCAAG TCGAAGTCAC ATTGACCTCC AAGATGCAGA CCTTGGCAAT GGGCATGAAG 
TTTGCACCAT CTGGGGAATT GTATGCTAAG GTTCCAATTC CTTGTTCCCT GACACCAGAC 
ACAGACCAGG GCTATTTGCT AAGTAGAGCT GTTAGCACTG CTTACGTGGC ACCTGTCCCT 
GCCCCTGACA ATCATGTGTA TGAGGTTCGG GTGGAAGTTA AAGCCACTTC AGAGCAGAAT 
GTGTGGCTCC TACTGCCAGC CCACTGCTGC TCTGCCTTGG GACTTAAGGA TGAAGACACA 
CAGCTTATGG AAATCCAGTT TCAAATCGAC AAGACTATAT TTGGGATATG GCACCAAGCC 
ATTGATGCAC TTCAGGATGA TAGACTGGTG GCACCTGATG TACCTGCCTG TTCTCTGCCT 
TCTTTGAGGC CCCCTCCCCA GGAACTCCGT GGTAATCCCA AGCAGAAGGA GGCCATCTCC 
TTTATCACAG GGACAACGAG TGGTCAGAGC CGAGTGCCTC CACTGCTCAT TTATGGCCCC 
TTTGGGACAG GCAAGACATA CACACTAGCT ATGGCCACGC TAGAGGTTAT CAAACAGCCA 
AACACAAAAG TGTTGATCTG TACTCACACC AACAGTGCTG CTGACATCTA CATCCGTGAG 
TACTTTCATG CTTATGTCAC AGCTGGACAC CCTGAGGCTG TGCCATTGAG GGTCAAAAAC 
ACTGAAAGCT TCCTCAACCA GACAGACCCG ACCACTCTGC AGTATTGCTG CCTCTCAAGT 
GATGGACGGT CCTTTTGCTT CCCAACTCAG GAGAAACTAA AGCAGCACCG CATTATAATC 
ACTACCACTA CCTTGTCCCG CTCCCTATGT GTGCCATCTG GGTTTTTTTC CCACATTCTC 
ATTGATGAGG CTGCTCAGAT GTTAGAATGT GAAGCCATCA TCCCACTGGC CTATGCTACC 
CAGAAGACAC GCATTGTCCT TGCCGGGGAC CACATGCAAA TCACCCCCAA ACTCTTCAGT 
GTTGGGGCCA GGAAGTCTGC TGACCATACA CTGCTTAATC GGCTGTTCCA GTACTATCAG 
AAGGAGACTC ATAAATTGGC TTCCCAGAGC AGGGTCATCT TCCATGAAAA CTACCGTTCC 
ACCAAAGCCA TCATTTCCTT TGTGTCTCGG AATTTCTATG TGGCTAAGGG TAACCCTATT 
CAGGCTAGCG GACAGATCCC TGCACACCCT TGCAAGTACC CACTCATGTT TTGCCATGTG 
GCTGGTACTC CTGAGAGAGA CATAACAATG ACATCATGGT TTAACAGGGC TGAGATAGTG 
GAGGTGATTG AGAAGGTGCA AGAATTCTAT CGTATCTGGG CCTACCAATG GAATAGCCCT 
GACCTAAAGC AAATTTGTGT TGTGTCCCAT GGTTCTCAGG TAAAAATATT GAGGCAGGAA 
CTGAGGAAGA AACAACTAGG AGAAGTAGCA GTGGAGAATT TTGAAAATCT GCCAGGTCGT 
GAATTCCGAG TGATCATCCT CAGCACAGTC CACACTCGGG ACAGCCTGCT CAACAGCTCA 
GTCCCCCACC TAGAATTCTT CAGTGAGGCT CGGGTGCTGA ACACCGTGAT GACCAGAGCC 
CAGTCCCAGG TGGTTGCTGT GGGCGATGCT GTGGCCCTTT GTTCTTTTGG ACGTTGTAGC 
AAGGTATGGA AGCACTTCAT CCAAGAGTGT ATAGAGATGC GCAGTGTGTC CCCTGTGGAC 
CTCACAATGG AACAAATCAA GCAAGATGTG GCACACAGGC AGCGCTGGGC CCGGCAAAAT 
GGACTGCATC CTATGAACTC TCCCAGGGAG CACTCGTTAG AAGAGGAAGA CAATGACAAT 
GACATTCCCT CATGGGTGTC TGATGCTGAG CTCAATGCTG ATGATCCCAT CCTCCAGGAG 
TTGCTGGATG AGAGTAAGAA TGTGGCAGTG ACAGTAAGTG AGGATGGGCT GCTGAATGTG 
ACATCAGGGT CCCGGTCCCG CTATCAGGGA CAACAGTATT TAAGCTTTCC TGTGCCAGTG 
TTGCAGAAAT ATCTCCGAAT GCAACCCAAG ATATATAGGA AATGTGAACT GATAAAGGAG 
GCCTTTGAGA AGGCATCTGC CTTCACCATA GATGACTCCC CACCCCTCAT TATTCAGATC 
AGAGGACGCA CCAACTGTGG AATGGCCTTC ACTGGGGATC AAGTTCTAGT GGAAATCCTG 
ACTCCAGAAT TGGAGGAACA GGGGTACTCC TGGCGCCCCA TGGGCCGAGT GGTAGGAATA 
CTAGAAAGAG GTGACCGTGA ACTCATCTTC ATTTGCAAAA TGGATGAGTT TGACCCCCGA 
GTTATGATCC CCATTGACCC GTCAGTCACC AAGATCTTTG TGCCAGAGTT AAAGGATAAG 
CCCAACACGA TTCCTATCCG CCGCCTACAA CGAGGACACA TCAAGCTTAT TAGCTATGAG 
AAGATAACAG AGGAGACCAA GCGTAATCAG CTCTTTCGTG TTCAGATCAT CTCTTGGAAA 
GAGAGGTTCT ATTATCCCTT AGGCATCATT CTTGAGATTC TTCCCTTGCC TTTGACCTTA 
GAACAAGGTT TAAAGATTCT GGACATGGAA TATTCTCTGA ATGCTAATGG AAGATACCCA 
ACTCCAGTAA TTAAGGAAGT GAACCAACAC AACTGTAGTA AATTGAATCT GCACCGGGGA 
CAGTGGAAGG ACTGCCGGGA TTATTTGACC TTTACTGTGG ACCCGCAGGG CTCCCGGGAT 
CTGGATGATG CTATCAGTGT CCGGGACCTG GGAAGCCATT ATGAAATTGC TGTCCATATA 
TCTGATGTGG CTAGCTTCAT GCCAAAGGAT GGTGCACTTG ACCGAGAGGC TAGAAAGCGA 
GGTGCTACCT ACTATGCTCC TGGAAAGGAT CCAGTGGCCA TGCTCCCTTC CAGGCTCAGC 
CAGGACCTGT TCAGTCTCCT TCCCCAGCAA GAACGTCGCA CTATCTCCCT GATTCTTACA 
GTGGAGAAGG AAAGTGACCG AGTAGTGCAA TGGCATATCA CACCATCTAT GATATGCTCT 
GACCTCCAAC TGACGTATGA GGAGGCTGAG GTTATTATTA AGAGTCATCC TGGGGGTGGG 
GCCTGGCTGC CCCTCCGCCT GGGCACTGTA GAAGGCTGTG TTGTGGCTGC TTATCACTTC 
TCCCAAGTCC TCCGCAGAAG CCGCCTTCAG GGAGATTGCT ACTATGACCA ACCAGATGAA 
GACAGCCCTC TCGGCTCACG TAGCTCCCAC CAGATGGTTG AGGAGCTTAT GATCCTATTC 
AATAGCTATG TAGCTGAATT CCTGGTGCAC CAGGATAACA CAAAGCTGAT CACCCCATTG 
AGGTGCCAGG CTGAGCCCAA TCCTCAGCAA GTAGCTCAGT TAAAGGATAA ATACAGTCAC 
TTGCTCCCTC TGTCCCTTCA TCTCTCTTAT CGTCTCTGTG GCCAGACCAC CAGCACTTTC 
CAAGGAACCA GTTCAGGATA CAACCTTTGC ATCCTGCGCC CGCTTTGGGA CCACCTGTGC 
CAAGCTGCTG ACAGCAAGGA CTATAACAAG ATGGTGGACT TAATTGCCAC AGATGACATC 
CACCCCACTC TGGCCCCAGC TGGACTGGAG TTCCGAAAGC TGCTCAGCCG GTCCTACTTT 
AGCCGCTCCA ACTCCACCAG CCTGTCCAGG GCTGGGCATT ACTCGTTGCA GGTGGACTGG 
TATACTTGGG CATCATCCCC CATAAGGCGC TATATTGATG TGGTGCTCCA GCGGCAGCTC 
CATGCTGCCA TGACCAAGGG CCATCCTGGA TATTCCCCTG AAGATATTGA CTTACTGTGC 
CATGACTTCA ACCGGAAGAA TGGACGCGCA CAGGCTTACG AGCGCAAGGC CCACTGCCTT 
CACCTTGCCA CCCAGCTCAA GACCCAGCTG CTGACAAAGC TGTGCTTTGT GGTAAATGTG 
GAATCTGCAA ACCGCTTTTT CAAGGTGATT TTCCCCATGA ACCGGGATAC CCTGCCTGAT 
CCCCACCTTG TCAATTATCG TGCACTGCAA CTGGTAGAGC AGCCCACCTT CATCCCTGAG 
CACCAGAAAA TGAAGTTGGT TTGGAAGAGG CGAATATACT CTCTGGATAA GGGCAAAGCA 
TGGATGCCAT CCTCTAAAAC CCTGGTAGAC ACCCATGTCA TGCCAGTAAG CAGTGTCATC 
TGGCAGGACC TGCTGGTTGC AATCCAGAGT CAGAATTTTC AGAAAGCAGC AGCCTTGCTC 
CTAAGGTGTC GAGGGACTGT TCAGACCTCC CACCATCGGT CCTTAGGCCA GGTGTCTCGA 
AGCAAGTGCT CGCACTATGT AGAGGTGATA GTAGAGCTAA GTACAGGGGA TGCCCTGCAG 
ATGCAACTGA CTACTGATGT CCAGAGGGGC TTCCTTGTGC CCTCCATTCA GATGTGGATG 
TTGACCCCAG GTTTTGCCAT TTGCCTGGAG CATGCTGAGA AACCCATTGA CTGCTTTTCC 
CGCTATGCTT CACAGACAAC GAAAGATGTG TATTCTGAGG CACAGGAATA TTCCCGGGTA 
TGGGAACCAC TCTGTGCCAT GGAGTCCGCC TCCAGTGCTG TGGCTGAGAA CGACTCTATT 
CTCTTGCATG GTGTTCCCAT TACTTGGGAC CAAGAGCGGA ATGACCAGGG ACAGATGCAA 
GGTTCCTTCT GCTTAACCTA TAGCTTCCTC AAGGAATGTG CGATTGACAT CAACTTTAAT 
TTCTGCTACC TCTGCATCCG GTTGGAGAAC CTGAAACTCC AGGGTCCAGT AGAAGGGGAG 
GAGGAGAGGG AAAAAGGAAA GGAGGCTGCT CTGTGTCAAG GCTTTAGGAA GCTCAGCTTG 
GGCCACACGG CAGCAAACCC ATCTGTGGTC AATACTCTTG ACTCTTGTTT GAATGTAGAC 
CCTGACACGT ATACCTGGGT GGCTCATGGG CTGACAGATG AAATGGATCA GGATTCCAAA 
AAAGTTGATC GAAGAGAAGA ACGACAGAGA ATCCATTTTT TCCTACATCA TGTAGCCATG 
GAGAGCATTC CCGTGGAGGT CACCAGAAAG GAAGCTTGCT TCACCATTGA GATGATCCCA 
AAGCTTCTGC CAGATATCCG GAAAGAGGAA GCCATCTGGA AACTGAAATA TGCCTCACCA 
CTTGTCACCA GCATTGCCTT AGGCCAGCCC ATTAAGGAAG TGTCCTGCGT AGCCATGAAG 
TCCAGTATCC TGAAGCGCCA GACCTTTGAC ATTCCCCGAG GAAAATTCAA GCTCAATAAG 
AGTCAGAACA GTGCTGTGCT AGAAGCTCTT CAAAAGCCTT TTACCCTCAT CCAAGGGCCA 
CCAGGCACTG GGAAGACGAT GGTCGGGGTC CATATTGTAT ACTGGTTCCA TAAGCTAAAC 
CAGGAGAAGC CTGAAACTGC TCCGCTGCCC TTGAGAGGGG AGAAGAAAGA CAAAGGGGAG 
AAGTGCATCC TATATTGTGG GCCATCCAAC AAATCTGTTG ATGTTGTTTC AGAAATCCTC 
CTTCAGTGGA AGGAGGAACT CAGACCATTG CAAGTATATA GTGAACAGAT GGAGGCCATG 
GAATTCCCTG TACCTGGGAG CTATAGAAAT ATGTTTCGCA AAAATCTCCG GGAAGGGAAG 
ACAAAACCTG AGCTCAGACA GATCATCCTT CATCATCGTA TCCGGATGCC GTCCAATCCA 
TATGCTCGCA AGATTATGGA ATTTGATACC AGAGTGAAGA TGGGGGATCA GTTTTCAGAG 
GAAGAGATTG AACTATATAA GTCCATCACC ACGACGGCCC GGAAATATGA ACTAAGCAAC 
CATGACATAA TCCTCTGTAC TTGCTCCTGT TCAGCCTCCT CCATTCTCAC CAAACAACTC 
AATGTCAAAC AAATTCTCAT TGATGAAGCT GGCATGTCCA CAGAGCCTGA AAGCCTCATC 
CCCCTTGTTA ACTTTCCCCG GGCTGAGAAG GTGGTGTTGC TAGGGGACCA CAAACAGCTT 
CAGCCTTTTG TAAAGAATGA TCACTGTCAA AACCTGGGCT TGGAGAAGTC CCTCTTCGAG 
CGTTACCATC GTCAAGCCTG GATGCTGGAC ACACAGTATC GAATGCACAA GGACATCTGC 
AAATTCCCTT CCAAAGAATT TTATGACAAT AAATTAAAGA CTAGCATGGA ACTAAGGCGA 
ACGCCAAGTG TCTTCAGCCA CCAAGATCAA GAAAGCTGTC CCATCATCTT TGGCTATGTC 
CAGGGAGAAG AGAAGAGTCT GGTAGTCTCC ACAGATGAAG GAAATGAAAA CTCCAAAGCT 
AACCAAGATG AAGTGGAGCA GGTGATTCGA ATAGCAAAGC AGCTGACCTT GGATAGGACC 
ATCAAACCCA AGGACATTGC AATCCTCAGC CCATACAATG CCCAGGTATC AGAGATCAAC 
AAGAGACTAT TAAAGGAGGG AATTACTGGA GTGACTGTCT CCTCCATCAA GAAGAGCCAA 
GGAAGTGAGT GGCGATATGT CATTCTAAGC ACAGTCCGCT CCTGCCCTCT TAGCGAAATT 
GATGGGAAGC CCACCAAAAG CTGGCTGAAG AAACACCTGG GTTTTGTCAG TGACCCACAT 
CAGGTCAATG TGGGGATCAC TCGGGCCCAA GAGGGGCTCT GCATCATTGG AAATCACTAC 
CTCTTGAGTT GCTGTCCAAT GTGGCATCGG CTCATTGATT TCTACACCAA GAAGGGATGC 
TGTGTCATGG CTTCCTCAGT GCAAGTGCGT CAGAGGCCAG CTATCTACCA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HELZ2 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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