GALC cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	GALC
Entrez Gene ID	100915302
Full Name	galactosylceramidase
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003758827.4	XP_003758875.1	galactocerebrosidase isoform X1
XM_023495389.1	XP_023351157.1	galactocerebrosidase isoform X3
XM_003758827.3	XP_003758875.1	galactocerebrosidase isoform X2
XM_031952407.1	XP_031808267.1	galactocerebrosidase isoform X2
XM_003758827.2	XP_003758875.1	galactocerebrosidase
XM_023495388.1	XP_023351156.1	galactocerebrosidase isoform X1

Pathways from BioSystems
Gene Ontology

The following GALC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GALC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa120589	XM_003758827.4 Latest version!	Sarcophilus harrisii galactosylceramidase (GALC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSa151187	XM_023495388.1 Latest version!	Sarcophilus harrisii galactosylceramidase (GALC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSa151188	XM_023495389.1 Latest version!	Sarcophilus harrisii galactosylceramidase (GALC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OSa225108	XM_031952407.1 Latest version!	Sarcophilus harrisii galactosylceramidase (GALC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OSa120589	XM_003758827.3	Sarcophilus harrisii galactosylceramidase (GALC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSa120589	XM_003758827.2	Sarcophilus harrisii galactosylceramidase (GALC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GALC ( XM_003758827.4 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003758875.1 Sarcophilus harrisii galactosylceramidase (GALC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa120589
Clone ID Related Accession (Same CDS sequence)	XM_003758827.4 , XM_003758827.3 , XM_003758827.2
Accession Version	XM_003758827.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	galactocerebrosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_003758827.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGC GCCAGATTGG GACTGGCGTC CCGGGGGCGG AGGGTCGGTC ACTTCTGCTG 
TTGCTGCTGC GGCTGCTTTC CTCGCTGCTG CTTCTGAGCC ATGCGGGAGC TTCCAGCTTA 
CGATACTTTG ACAATTACAG TCTGCGCCCA GACCCCAGTT ACGTCCTGGA GGATGTCGCC 
GGCCTTGGTC GGGTGTTTGA CGGCATCGGC GGGCTGAGCG GCGGCGGGGC AACCTCCCGA 
CTTCTAGTCA ATTATCCTGA GCCATATCGT TCTGAAATAT TGGATTATTT ATTTAAGCCA 
AACTTTGGTG CATCTTTGCA TATTCTGAAA GTGGAAATAG GAGGTGATGG ACAAACAACA 
GATGGCACTG AACCCTCTCA TATGCATTAT GCAAATGATG AGAATTATTT CCGGGGCTAT 
GAATGGTGGC TAATGAAGGA AGCTAAGAAG AGGAATCCCA ATATTAAGCT TATTGGGTTG 
CCGTGGTCAT TTCCTGGTTG GATAGGTCAC GGTCAAAACT GGCCCTATGC CCAACCCCAA 
ACTACTGCTC TATATGTTGT GTCTTGGATA TTGGGTGCTA AACAACATCA CAATTTGGAT 
ATTGATTATA TTGGGATTTG GAATGAGAGA ACATTTGACA GTAAATATAT AAAGGTATTA 
AGAGAAGTGC TTTCTCAGAA TGGCCTGAAA AGAGTGAAGA TCATAGCCAG TGATTACCTC 
TGGCTGCCCA TTTCCATTGC TATGCTACAT GATTATGAAC TCATGAAAGC TGTCGATGTT 
ATCGGGGCTC ATTATCCTGG AACTCATACT ATACCTGAAG CACTGAAGAC CCAAAAAATA 
CTTTGGTCTT CAGAAGATTA CAGCACTTTC AATGATGAAG TGGGTGGAGG CTGCTGGGCC 
CGTATTTTGA ATCAGAACTA TGTGAATGGC AACATGACTG CCACAATTGC TTGGAATTTG 
GTGGCTAGTT ACTACCAGCA ACTACCTTTT GGGAGAGATG GATTGATGAC TGCTCAGGAG 
CCTTGGAGTG GACATTATGT TGTAGAGAGT CCTATCTGGA TTTCAGCTCA CACCACTCAA 
TTTGCTCAAC CAGGATGGCA ATATCTAAAA ACAGTTGGCC ATTTAGTGAA AGGTGGAAGC 
TATGTTGCTC TTACTGATGG CTTAGGTAAC CTCACTATTA TCATTGAAAC CATGACTCAT 
AAGCATTCAA AATGCATAAG GCCACCTCTT CCTTTCTTCA ATGTGTCAGA ACAAATTGCA 
AATTTCTCTC TTAGTGGATC CTTTAAACAA TTAAGAACGT TACAGGTGTG GTATTCCAAA 
CTAGGGGACA AAACTGGAAA TAACTCTATC TTATTCAAAC AACTACCTCC TCTAAAGATT 
AATGATGGTA CAGTCACATT AAAATTGGGT CTGGATGAAG TATATACTTT AACAACTATC 
ACTACAGGTC AGAAAGGCAG TTATCCTATT CCTCCAGAGT CACAACCATT TCCAACAAGC 
TATAAAGATG ATTTCAACAT TCGTACTCCA CCTTTTAGTG AAGCTCCGAA TTTTGCTGAT 
CAGACGGGAG TTTTTGAGTA CTTCACCAAT CCCGAGCAAG GGGGGGACCA TGCCTTCACT 
TTACGCCAAG TGCTCACTCA GCGACCTATC ACCTGGGCTG CTGATGCAGA TCAGACCATC 
AGTGTTATAG GGGATTATAA CTGGAAGGAT CTGGTAATAA GTTGTGATAT CTACATAGAG 
ACACCACATA GTGGTGGTGT ATTCATTGCT GGAAGAGTAA ATAAAGGTGG GGAAGTCATC 
CGAAGCGCTG CAGGAATTTT CTTCTGGGTT TTTGCAGATG GGACCTACCA AGTTACAAAT 
GATCTTGCTA GACTGTTGAT ATTAAAGAAA GGACATGCTA ATGTTAGTGC TGGGAAATGG 
TACACCCTCA CTCTGAATAT TAAAGACTCT TCTGTTAATG TCATACTAGA TAATACACAT 
CTCTGGGAAG GTGTGCTACC CTTATATCCC AAGAATGGTT GGGCAGCAAT TGGAACACAT 
AGCTTTGAGT TTGCACAGTT TGACAACTTT CATATAGAGG CTACAAATGT GCATTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003758875.1
CDS	72..2171
Translation	MKTRQIGTGVPGAEGRSLLLLLLRLLSSLLLLSHAGASSLRYFDNYSLRPDPSYVLEDVAGLGRVFDGIGGLSGGGATSRLLVNYPEPYRSEILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYANDENYFRGYEWWLMKEAKKRNPNIKLIGLPWSFPGWIGHGQNWPYAQPQTTALYVVSWILGAKQHHNLDIDYIGIWNERTFDSKYIKVLREVLSQNGLKRVKIIASDYLWLPISIAMLHDYELMKAVDVIGAHYPGTHTIPEALKTQKILWSSEDYSTFNDEVGGGCWARILNQNYVNGNMTATIAWNLVASYYQQLPFGRDGLMTAQEPWSGHYVVESPIWISAHTTQFAQPGWQYLKTVGHLVKGGSYVALTDGLGNLTIIIETMTHKHSKCIRPPLPFFNVSEQIANFSLSGSFKQLRTLQVWYSKLGDKTGNNSILFKQLPPLKINDGTVTLKLGLDEVYTLTTITTGQKGSYPIPPESQPFPTSYKDDFNIRTPPFSEAPNFADQTGVFEYFTNPEQGGDHAFTLRQVLTQRPITWAADADQTISVIGDYNWKDLVISCDIYIETPHSGGVFIAGRVNKGGEVIRSAAGIFFWVFADGTYQVTNDLARLLILKKGHANVSAGKWYTLTLNIKDSSVNVILDNTHLWEGVLPLYPKNGWAAIGTHSFEFAQFDNFHIEATNVHS

Target ORF information:

RefSeq Version	XM_003758827.4
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii galactosylceramidase (GALC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003758827.4

ORF Insert Sequence:

ATGAAGACGC GCCAGATTGG GACTGGCGTC CCGGGGGCGG AGGGTCGGTC ACTTCTGCTG 
TTGCTGCTGC GGCTGCTTTC CTCGCTGCTG CTTCTGAGCC ATGCGGGAGC TTCCAGCTTA 
CGATACTTTG ACAATTACAG TCTGCGCCCA GACCCCAGTT ACGTCCTGGA GGATGTCGCC 
GGCCTTGGTC GGGTGTTTGA CGGCATCGGC GGGCTGAGCG GCGGCGGGGC AACCTCCCGA 
CTTCTAGTCA ATTATCCTGA GCCATATCGT TCTGAAATAT TGGATTATTT ATTTAAGCCA 
AACTTTGGTG CATCTTTGCA TATTCTGAAA GTGGAAATAG GAGGTGATGG ACAAACAACA 
GATGGCACTG AACCCTCTCA TATGCATTAT GCAAATGATG AGAATTATTT CCGGGGCTAT 
GAATGGTGGC TAATGAAGGA AGCTAAGAAG AGGAATCCCA ATATTAAGCT TATTGGGTTG 
CCGTGGTCAT TTCCTGGTTG GATAGGTCAC GGTCAAAACT GGCCCTATGC CCAACCCCAA 
ACTACTGCTC TATATGTTGT GTCTTGGATA TTGGGTGCTA AACAACATCA CAATTTGGAT 
ATTGATTATA TTGGGATTTG GAATGAGAGA ACATTTGACA GTAAATATAT AAAGGTATTA 
AGAGAAGTGC TTTCTCAGAA TGGCCTGAAA AGAGTGAAGA TCATAGCCAG TGATTACCTC 
TGGCTGCCCA TTTCCATTGC TATGCTACAT GATTATGAAC TCATGAAAGC TGTCGATGTT 
ATCGGGGCTC ATTATCCTGG AACTCATACT ATACCTGAAG CACTGAAGAC CCAAAAAATA 
CTTTGGTCTT CAGAAGATTA CAGCACTTTC AATGATGAAG TGGGTGGAGG CTGCTGGGCC 
CGTATTTTGA ATCAGAACTA TGTGAATGGC AACATGACTG CCACAATTGC TTGGAATTTG 
GTGGCTAGTT ACTACCAGCA ACTACCTTTT GGGAGAGATG GATTGATGAC TGCTCAGGAG 
CCTTGGAGTG GACATTATGT TGTAGAGAGT CCTATCTGGA TTTCAGCTCA CACCACTCAA 
TTTGCTCAAC CAGGATGGCA ATATCTAAAA ACAGTTGGCC ATTTAGTGAA AGGTGGAAGC 
TATGTTGCTC TTACTGATGG CTTAGGTAAC CTCACTATTA TCATTGAAAC CATGACTCAT 
AAGCATTCAA AATGCATAAG GCCACCTCTT CCTTTCTTCA ATGTGTCAGA ACAAATTGCA 
AATTTCTCTC TTAGTGGATC CTTTAAACAA TTAAGAACGT TACAGGTGTG GTATTCCAAA 
CTAGGGGACA AAACTGGAAA TAACTCTATC TTATTCAAAC AACTACCTCC TCTAAAGATT 
AATGATGGTA CAGTCACATT AAAATTGGGT CTGGATGAAG TATATACTTT AACAACTATC 
ACTACAGGTC AGAAAGGCAG TTATCCTATT CCTCCAGAGT CACAACCATT TCCAACAAGC 
TATAAAGATG ATTTCAACAT TCGTACTCCA CCTTTTAGTG AAGCTCCGAA TTTTGCTGAT 
CAGACGGGAG TTTTTGAGTA CTTCACCAAT CCCGAGCAAG GGGGGGACCA TGCCTTCACT 
TTACGCCAAG TGCTCACTCA GCGACCTATC ACCTGGGCTG CTGATGCAGA TCAGACCATC 
AGTGTTATAG GGGATTATAA CTGGAAGGAT CTGGTAATAA GTTGTGATAT CTACATAGAG 
ACACCACATA GTGGTGGTGT ATTCATTGCT GGAAGAGTAA ATAAAGGTGG GGAAGTCATC 
CGAAGCGCTG CAGGAATTTT CTTCTGGGTT TTTGCAGATG GGACCTACCA AGTTACAAAT 
GATCTTGCTA GACTGTTGAT ATTAAAGAAA GGACATGCTA ATGTTAGTGC TGGGAAATGG 
TACACCCTCA CTCTGAATAT TAAAGACTCT TCTGTTAATG TCATACTAGA TAATACACAT 
CTCTGGGAAG GTGTGCTACC CTTATATCCC AAGAATGGTT GGGCAGCAAT TGGAACACAT 
AGCTTTGAGT TTGCACAGTT TGACAACTTT CATATAGAGG CTACAAATGT GCATTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GALC ( XM_003758827.3 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003758875.1 Sarcophilus harrisii galactosylceramidase (GALC), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa120589
Clone ID Related Accession (Same CDS sequence)	XM_003758827.4 , XM_003758827.3 , XM_003758827.2
Accession Version	XM_003758827.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	galactocerebrosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_003758827.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGC GCCAGATTGG GACTGGCGTC CCGGGGGCGG AGGGTCGGTC ACTTCTGCTG 
TTGCTGCTGC GGCTGCTTTC CTCGCTGCTG CTTCTGAGCC ATGCGGGAGC TTCCAGCTTA 
CGATACTTTG ACAATTACAG TCTGCGCCCA GACCCCAGTT ACGTCCTGGA GGATGTCGCC 
GGCCTTGGTC GGGTGTTTGA CGGCATCGGC GGGCTGAGCG GCGGCGGGGC AACCTCCCGA 
CTTCTAGTCA ATTATCCTGA GCCATATCGT TCTGAAATAT TGGATTATTT ATTTAAGCCA 
AACTTTGGTG CATCTTTGCA TATTCTGAAA GTGGAAATAG GAGGTGATGG ACAAACAACA 
GATGGCACTG AACCCTCTCA TATGCATTAT GCAAATGATG AGAATTATTT CCGGGGCTAT 
GAATGGTGGC TAATGAAGGA AGCTAAGAAG AGGAATCCCA ATATTAAGCT TATTGGGTTG 
CCGTGGTCAT TTCCTGGTTG GATAGGTCAC GGTCAAAACT GGCCCTATGC CCAACCCCAA 
ACTACTGCTC TATATGTTGT GTCTTGGATA TTGGGTGCTA AACAACATCA CAATTTGGAT 
ATTGATTATA TTGGGATTTG GAATGAGAGA ACATTTGACA GTAAATATAT AAAGGTATTA 
AGAGAAGTGC TTTCTCAGAA TGGCCTGAAA AGAGTGAAGA TCATAGCCAG TGATTACCTC 
TGGCTGCCCA TTTCCATTGC TATGCTACAT GATTATGAAC TCATGAAAGC TGTCGATGTT 
ATCGGGGCTC ATTATCCTGG AACTCATACT ATACCTGAAG CACTGAAGAC CCAAAAAATA 
CTTTGGTCTT CAGAAGATTA CAGCACTTTC AATGATGAAG TGGGTGGAGG CTGCTGGGCC 
CGTATTTTGA ATCAGAACTA TGTGAATGGC AACATGACTG CCACAATTGC TTGGAATTTG 
GTGGCTAGTT ACTACCAGCA ACTACCTTTT GGGAGAGATG GATTGATGAC TGCTCAGGAG 
CCTTGGAGTG GACATTATGT TGTAGAGAGT CCTATCTGGA TTTCAGCTCA CACCACTCAA 
TTTGCTCAAC CAGGATGGCA ATATCTAAAA ACAGTTGGCC ATTTAGTGAA AGGTGGAAGC 
TATGTTGCTC TTACTGATGG CTTAGGTAAC CTCACTATTA TCATTGAAAC CATGACTCAT 
AAGCATTCAA AATGCATAAG GCCACCTCTT CCTTTCTTCA ATGTGTCAGA ACAAATTGCA 
AATTTCTCTC TTAGTGGATC CTTTAAACAA TTAAGAACGT TACAGGTGTG GTATTCCAAA 
CTAGGGGACA AAACTGGAAA TAACTCTATC TTATTCAAAC AACTACCTCC TCTAAAGATT 
AATGATGGTA CAGTCACATT AAAATTGGGT CTGGATGAAG TATATACTTT AACAACTATC 
ACTACAGGTC AGAAAGGCAG TTATCCTATT CCTCCAGAGT CACAACCATT TCCAACAAGC 
TATAAAGATG ATTTCAACAT TCGTACTCCA CCTTTTAGTG AAGCTCCGAA TTTTGCTGAT 
CAGACGGGAG TTTTTGAGTA CTTCACCAAT CCCGAGCAAG GGGGGGACCA TGCCTTCACT 
TTACGCCAAG TGCTCACTCA GCGACCTATC ACCTGGGCTG CTGATGCAGA TCAGACCATC 
AGTGTTATAG GGGATTATAA CTGGAAGGAT CTGGTAATAA GTTGTGATAT CTACATAGAG 
ACACCACATA GTGGTGGTGT ATTCATTGCT GGAAGAGTAA ATAAAGGTGG GGAAGTCATC 
CGAAGCGCTG CAGGAATTTT CTTCTGGGTT TTTGCAGATG GGACCTACCA AGTTACAAAT 
GATCTTGCTA GACTGTTGAT ATTAAAGAAA GGACATGCTA ATGTTAGTGC TGGGAAATGG 
TACACCCTCA CTCTGAATAT TAAAGACTCT TCTGTTAATG TCATACTAGA TAATACACAT 
CTCTGGGAAG GTGTGCTACC CTTATATCCC AAGAATGGTT GGGCAGCAAT TGGAACACAT 
AGCTTTGAGT TTGCACAGTT TGACAACTTT CATATAGAGG CTACAAATGT GCATTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003758875.1
CDS	59..2158
Translation	MKTRQIGTGVPGAEGRSLLLLLLRLLSSLLLLSHAGASSLRYFDNYSLRPDPSYVLEDVAGLGRVFDGIGGLSGGGATSRLLVNYPEPYRSEILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYANDENYFRGYEWWLMKEAKKRNPNIKLIGLPWSFPGWIGHGQNWPYAQPQTTALYVVSWILGAKQHHNLDIDYIGIWNERTFDSKYIKVLREVLSQNGLKRVKIIASDYLWLPISIAMLHDYELMKAVDVIGAHYPGTHTIPEALKTQKILWSSEDYSTFNDEVGGGCWARILNQNYVNGNMTATIAWNLVASYYQQLPFGRDGLMTAQEPWSGHYVVESPIWISAHTTQFAQPGWQYLKTVGHLVKGGSYVALTDGLGNLTIIIETMTHKHSKCIRPPLPFFNVSEQIANFSLSGSFKQLRTLQVWYSKLGDKTGNNSILFKQLPPLKINDGTVTLKLGLDEVYTLTTITTGQKGSYPIPPESQPFPTSYKDDFNIRTPPFSEAPNFADQTGVFEYFTNPEQGGDHAFTLRQVLTQRPITWAADADQTISVIGDYNWKDLVISCDIYIETPHSGGVFIAGRVNKGGEVIRSAAGIFFWVFADGTYQVTNDLARLLILKKGHANVSAGKWYTLTLNIKDSSVNVILDNTHLWEGVLPLYPKNGWAAIGTHSFEFAQFDNFHIEATNVHS

Target ORF information:

RefSeq Version	XM_003758827.3
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii galactosylceramidase (GALC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003758827.3

ORF Insert Sequence:

ATGAAGACGC GCCAGATTGG GACTGGCGTC CCGGGGGCGG AGGGTCGGTC ACTTCTGCTG 
TTGCTGCTGC GGCTGCTTTC CTCGCTGCTG CTTCTGAGCC ATGCGGGAGC TTCCAGCTTA 
CGATACTTTG ACAATTACAG TCTGCGCCCA GACCCCAGTT ACGTCCTGGA GGATGTCGCC 
GGCCTTGGTC GGGTGTTTGA CGGCATCGGC GGGCTGAGCG GCGGCGGGGC AACCTCCCGA 
CTTCTAGTCA ATTATCCTGA GCCATATCGT TCTGAAATAT TGGATTATTT ATTTAAGCCA 
AACTTTGGTG CATCTTTGCA TATTCTGAAA GTGGAAATAG GAGGTGATGG ACAAACAACA 
GATGGCACTG AACCCTCTCA TATGCATTAT GCAAATGATG AGAATTATTT CCGGGGCTAT 
GAATGGTGGC TAATGAAGGA AGCTAAGAAG AGGAATCCCA ATATTAAGCT TATTGGGTTG 
CCGTGGTCAT TTCCTGGTTG GATAGGTCAC GGTCAAAACT GGCCCTATGC CCAACCCCAA 
ACTACTGCTC TATATGTTGT GTCTTGGATA TTGGGTGCTA AACAACATCA CAATTTGGAT 
ATTGATTATA TTGGGATTTG GAATGAGAGA ACATTTGACA GTAAATATAT AAAGGTATTA 
AGAGAAGTGC TTTCTCAGAA TGGCCTGAAA AGAGTGAAGA TCATAGCCAG TGATTACCTC 
TGGCTGCCCA TTTCCATTGC TATGCTACAT GATTATGAAC TCATGAAAGC TGTCGATGTT 
ATCGGGGCTC ATTATCCTGG AACTCATACT ATACCTGAAG CACTGAAGAC CCAAAAAATA 
CTTTGGTCTT CAGAAGATTA CAGCACTTTC AATGATGAAG TGGGTGGAGG CTGCTGGGCC 
CGTATTTTGA ATCAGAACTA TGTGAATGGC AACATGACTG CCACAATTGC TTGGAATTTG 
GTGGCTAGTT ACTACCAGCA ACTACCTTTT GGGAGAGATG GATTGATGAC TGCTCAGGAG 
CCTTGGAGTG GACATTATGT TGTAGAGAGT CCTATCTGGA TTTCAGCTCA CACCACTCAA 
TTTGCTCAAC CAGGATGGCA ATATCTAAAA ACAGTTGGCC ATTTAGTGAA AGGTGGAAGC 
TATGTTGCTC TTACTGATGG CTTAGGTAAC CTCACTATTA TCATTGAAAC CATGACTCAT 
AAGCATTCAA AATGCATAAG GCCACCTCTT CCTTTCTTCA ATGTGTCAGA ACAAATTGCA 
AATTTCTCTC TTAGTGGATC CTTTAAACAA TTAAGAACGT TACAGGTGTG GTATTCCAAA 
CTAGGGGACA AAACTGGAAA TAACTCTATC TTATTCAAAC AACTACCTCC TCTAAAGATT 
AATGATGGTA CAGTCACATT AAAATTGGGT CTGGATGAAG TATATACTTT AACAACTATC 
ACTACAGGTC AGAAAGGCAG TTATCCTATT CCTCCAGAGT CACAACCATT TCCAACAAGC 
TATAAAGATG ATTTCAACAT TCGTACTCCA CCTTTTAGTG AAGCTCCGAA TTTTGCTGAT 
CAGACGGGAG TTTTTGAGTA CTTCACCAAT CCCGAGCAAG GGGGGGACCA TGCCTTCACT 
TTACGCCAAG TGCTCACTCA GCGACCTATC ACCTGGGCTG CTGATGCAGA TCAGACCATC 
AGTGTTATAG GGGATTATAA CTGGAAGGAT CTGGTAATAA GTTGTGATAT CTACATAGAG 
ACACCACATA GTGGTGGTGT ATTCATTGCT GGAAGAGTAA ATAAAGGTGG GGAAGTCATC 
CGAAGCGCTG CAGGAATTTT CTTCTGGGTT TTTGCAGATG GGACCTACCA AGTTACAAAT 
GATCTTGCTA GACTGTTGAT ATTAAAGAAA GGACATGCTA ATGTTAGTGC TGGGAAATGG 
TACACCCTCA CTCTGAATAT TAAAGACTCT TCTGTTAATG TCATACTAGA TAATACACAT 
CTCTGGGAAG GTGTGCTACC CTTATATCCC AAGAATGGTT GGGCAGCAAT TGGAACACAT 
AGCTTTGAGT TTGCACAGTT TGACAACTTT CATATAGAGG CTACAAATGT GCATTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GALC ( XM_003758827.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003758875.1 Sarcophilus harrisii galactosylceramidase (GALC), mRNA.

Price & Availability↑

CloneID	OSa120589
Clone ID Related Accession (Same CDS sequence)	XM_003758827.4 , XM_003758827.3 , XM_003758827.2
Accession Version	XM_003758827.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	galactocerebrosidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 15, 2015 this sequence version replaced XM_003758827.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGC GCCAGATTGG GACTGGCGTC CCGGGGGCGG AGGGTCGGTC ACTTCTGCTG 
TTGCTGCTGC GGCTGCTTTC CTCGCTGCTG CTTCTGAGCC ATGCGGGAGC TTCCAGCTTA 
CGATACTTTG ACAATTACAG TCTGCGCCCA GACCCCAGTT ACGTCCTGGA GGATGTCGCC 
GGCCTTGGTC GGGTGTTTGA CGGCATCGGC GGGCTGAGCG GCGGCGGGGC AACCTCCCGA 
CTTCTAGTCA ATTATCCTGA GCCATATCGT TCTGAAATAT TGGATTATTT ATTTAAGCCA 
AACTTTGGTG CATCTTTGCA TATTCTGAAA GTGGAAATAG GAGGTGATGG ACAAACAACA 
GATGGCACTG AACCCTCTCA TATGCATTAT GCAAATGATG AGAATTATTT CCGGGGCTAT 
GAATGGTGGC TAATGAAGGA AGCTAAGAAG AGGAATCCCA ATATTAAGCT TATTGGGTTG 
CCGTGGTCAT TTCCTGGTTG GATAGGTCAC GGTCAAAACT GGCCCTATGC CCAACCCCAA 
ACTACTGCTC TATATGTTGT GTCTTGGATA TTGGGTGCTA AACAACATCA CAATTTGGAT 
ATTGATTATA TTGGGATTTG GAATGAGAGA ACATTTGACA GTAAATATAT AAAGGTATTA 
AGAGAAGTGC TTTCTCAGAA TGGCCTGAAA AGAGTGAAGA TCATAGCCAG TGATTACCTC 
TGGCTGCCCA TTTCCATTGC TATGCTACAT GATTATGAAC TCATGAAAGC TGTCGATGTT 
ATCGGGGCTC ATTATCCTGG AACTCATACT ATACCTGAAG CACTGAAGAC CCAAAAAATA 
CTTTGGTCTT CAGAAGATTA CAGCACTTTC AATGATGAAG TGGGTGGAGG CTGCTGGGCC 
CGTATTTTGA ATCAGAACTA TGTGAATGGC AACATGACTG CCACAATTGC TTGGAATTTG 
GTGGCTAGTT ACTACCAGCA ACTACCTTTT GGGAGAGATG GATTGATGAC TGCTCAGGAG 
CCTTGGAGTG GACATTATGT TGTAGAGAGT CCTATCTGGA TTTCAGCTCA CACCACTCAA 
TTTGCTCAAC CAGGATGGCA ATATCTAAAA ACAGTTGGCC ATTTAGTGAA AGGTGGAAGC 
TATGTTGCTC TTACTGATGG CTTAGGTAAC CTCACTATTA TCATTGAAAC CATGACTCAT 
AAGCATTCAA AATGCATAAG GCCACCTCTT CCTTTCTTCA ATGTGTCAGA ACAAATTGCA 
AATTTCTCTC TTAGTGGATC CTTTAAACAA TTAAGAACGT TACAGGTGTG GTATTCCAAA 
CTAGGGGACA AAACTGGAAA TAACTCTATC TTATTCAAAC AACTACCTCC TCTAAAGATT 
AATGATGGTA CAGTCACATT AAAATTGGGT CTGGATGAAG TATATACTTT AACAACTATC 
ACTACAGGTC AGAAAGGCAG TTATCCTATT CCTCCAGAGT CACAACCATT TCCAACAAGC 
TATAAAGATG ATTTCAACAT TCGTACTCCA CCTTTTAGTG AAGCTCCGAA TTTTGCTGAT 
CAGACGGGAG TTTTTGAGTA CTTCACCAAT CCCGAGCAAG GGGGGGACCA TGCCTTCACT 
TTACGCCAAG TGCTCACTCA GCGACCTATC ACCTGGGCTG CTGATGCAGA TCAGACCATC 
AGTGTTATAG GGGATTATAA CTGGAAGGAT CTGGTAATAA GTTGTGATAT CTACATAGAG 
ACACCACATA GTGGTGGTGT ATTCATTGCT GGAAGAGTAA ATAAAGGTGG GGAAGTCATC 
CGAAGCGCTG CAGGAATTTT CTTCTGGGTT TTTGCAGATG GGACCTACCA AGTTACAAAT 
GATCTTGCTA GACTGTTGAT ATTAAAGAAA GGACATGCTA ATGTTAGTGC TGGGAAATGG 
TACACCCTCA CTCTGAATAT TAAAGACTCT TCTGTTAATG TCATACTAGA TAATACACAT 
CTCTGGGAAG GTGTGCTACC CTTATATCCC AAGAATGGTT GGGCAGCAAT TGGAACACAT 
AGCTTTGAGT TTGCACAGTT TGACAACTTT CATATAGAGG CTACAAATGT GCATTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003758875.1
CDS	35..2134
Translation	MKTRQIGTGVPGAEGRSLLLLLLRLLSSLLLLSHAGASSLRYFDNYSLRPDPSYVLEDVAGLGRVFDGIGGLSGGGATSRLLVNYPEPYRSEILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYANDENYFRGYEWWLMKEAKKRNPNIKLIGLPWSFPGWIGHGQNWPYAQPQTTALYVVSWILGAKQHHNLDIDYIGIWNERTFDSKYIKVLREVLSQNGLKRVKIIASDYLWLPISIAMLHDYELMKAVDVIGAHYPGTHTIPEALKTQKILWSSEDYSTFNDEVGGGCWARILNQNYVNGNMTATIAWNLVASYYQQLPFGRDGLMTAQEPWSGHYVVESPIWISAHTTQFAQPGWQYLKTVGHLVKGGSYVALTDGLGNLTIIIETMTHKHSKCIRPPLPFFNVSEQIANFSLSGSFKQLRTLQVWYSKLGDKTGNNSILFKQLPPLKINDGTVTLKLGLDEVYTLTTITTGQKGSYPIPPESQPFPTSYKDDFNIRTPPFSEAPNFADQTGVFEYFTNPEQGGDHAFTLRQVLTQRPITWAADADQTISVIGDYNWKDLVISCDIYIETPHSGGVFIAGRVNKGGEVIRSAAGIFFWVFADGTYQVTNDLARLLILKKGHANVSAGKWYTLTLNIKDSSVNVILDNTHLWEGVLPLYPKNGWAAIGTHSFEFAQFDNFHIEATNVHS

Target ORF information:

RefSeq Version	XM_003758827.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii galactosylceramidase (GALC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003758827.2

ORF Insert Sequence:

ATGAAGACGC GCCAGATTGG GACTGGCGTC CCGGGGGCGG AGGGTCGGTC ACTTCTGCTG 
TTGCTGCTGC GGCTGCTTTC CTCGCTGCTG CTTCTGAGCC ATGCGGGAGC TTCCAGCTTA 
CGATACTTTG ACAATTACAG TCTGCGCCCA GACCCCAGTT ACGTCCTGGA GGATGTCGCC 
GGCCTTGGTC GGGTGTTTGA CGGCATCGGC GGGCTGAGCG GCGGCGGGGC AACCTCCCGA 
CTTCTAGTCA ATTATCCTGA GCCATATCGT TCTGAAATAT TGGATTATTT ATTTAAGCCA 
AACTTTGGTG CATCTTTGCA TATTCTGAAA GTGGAAATAG GAGGTGATGG ACAAACAACA 
GATGGCACTG AACCCTCTCA TATGCATTAT GCAAATGATG AGAATTATTT CCGGGGCTAT 
GAATGGTGGC TAATGAAGGA AGCTAAGAAG AGGAATCCCA ATATTAAGCT TATTGGGTTG 
CCGTGGTCAT TTCCTGGTTG GATAGGTCAC GGTCAAAACT GGCCCTATGC CCAACCCCAA 
ACTACTGCTC TATATGTTGT GTCTTGGATA TTGGGTGCTA AACAACATCA CAATTTGGAT 
ATTGATTATA TTGGGATTTG GAATGAGAGA ACATTTGACA GTAAATATAT AAAGGTATTA 
AGAGAAGTGC TTTCTCAGAA TGGCCTGAAA AGAGTGAAGA TCATAGCCAG TGATTACCTC 
TGGCTGCCCA TTTCCATTGC TATGCTACAT GATTATGAAC TCATGAAAGC TGTCGATGTT 
ATCGGGGCTC ATTATCCTGG AACTCATACT ATACCTGAAG CACTGAAGAC CCAAAAAATA 
CTTTGGTCTT CAGAAGATTA CAGCACTTTC AATGATGAAG TGGGTGGAGG CTGCTGGGCC 
CGTATTTTGA ATCAGAACTA TGTGAATGGC AACATGACTG CCACAATTGC TTGGAATTTG 
GTGGCTAGTT ACTACCAGCA ACTACCTTTT GGGAGAGATG GATTGATGAC TGCTCAGGAG 
CCTTGGAGTG GACATTATGT TGTAGAGAGT CCTATCTGGA TTTCAGCTCA CACCACTCAA 
TTTGCTCAAC CAGGATGGCA ATATCTAAAA ACAGTTGGCC ATTTAGTGAA AGGTGGAAGC 
TATGTTGCTC TTACTGATGG CTTAGGTAAC CTCACTATTA TCATTGAAAC CATGACTCAT 
AAGCATTCAA AATGCATAAG GCCACCTCTT CCTTTCTTCA ATGTGTCAGA ACAAATTGCA 
AATTTCTCTC TTAGTGGATC CTTTAAACAA TTAAGAACGT TACAGGTGTG GTATTCCAAA 
CTAGGGGACA AAACTGGAAA TAACTCTATC TTATTCAAAC AACTACCTCC TCTAAAGATT 
AATGATGGTA CAGTCACATT AAAATTGGGT CTGGATGAAG TATATACTTT AACAACTATC 
ACTACAGGTC AGAAAGGCAG TTATCCTATT CCTCCAGAGT CACAACCATT TCCAACAAGC 
TATAAAGATG ATTTCAACAT TCGTACTCCA CCTTTTAGTG AAGCTCCGAA TTTTGCTGAT 
CAGACGGGAG TTTTTGAGTA CTTCACCAAT CCCGAGCAAG GGGGGGACCA TGCCTTCACT 
TTACGCCAAG TGCTCACTCA GCGACCTATC ACCTGGGCTG CTGATGCAGA TCAGACCATC 
AGTGTTATAG GGGATTATAA CTGGAAGGAT CTGGTAATAA GTTGTGATAT CTACATAGAG 
ACACCACATA GTGGTGGTGT ATTCATTGCT GGAAGAGTAA ATAAAGGTGG GGAAGTCATC 
CGAAGCGCTG CAGGAATTTT CTTCTGGGTT TTTGCAGATG GGACCTACCA AGTTACAAAT 
GATCTTGCTA GACTGTTGAT ATTAAAGAAA GGACATGCTA ATGTTAGTGC TGGGAAATGG 
TACACCCTCA CTCTGAATAT TAAAGACTCT TCTGTTAATG TCATACTAGA TAATACACAT 
CTCTGGGAAG GTGTGCTACC CTTATATCCC AAGAATGGTT GGGCAGCAAT TGGAACACAT 
AGCTTTGAGT TTGCACAGTT TGACAACTTT CATATAGAGG CTACAAATGT GCATTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GALC ( XM_023495388.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023351156.1 Sarcophilus harrisii galactosylceramidase (GALC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa151187
Clone ID Related Accession (Same CDS sequence)	XM_023495388.1
Accession Version	XM_023495388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	galactocerebrosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGC GCCAGATTGG GACTGGCGTC CCGGGGGCGG AGGGTCGGTC ACTTCTGCTG 
TTGCTGCTGC GGCTGCTTTC CTCGCTGCTG CTTCTGAGCC ATGCGGGAGC TTCCAGCTTA 
CGATACTTTG ACAATTACAG TCTGCGCCCA GACCCCAGTT ACGTCCTGGA GGATGTCGCC 
GGCCTTGGTC GGGTGTTTGA CGGCATCGGC GGGCTGAGCG GCGGCGGGAC TTCCTTGGAT 
TTGTTTTCTT TCCCCTTTGA AATACAAGCT TCTTATGAGC AGGCAACCTC CCGACTTCTA 
GTCAATTATC CTGAGCCATA TCGTTCTGAA ATATTGGATT ATTTATTTAA GCCAAACTTT 
GGTGCATCTT TGCATATTCT GAAAGTGGAA ATAGGAGGTG ATGGACAAAC AACAGATGGC 
ACTGAACCCT CTCATATGCA TTATGCAAAT GATGAGAATT ATTTCCGGGG CTATGAATGG 
TGGCTAATGA AGGAAGCTAA GAAGAGGAAT CCCAATATTA AGCTTATTGG GTTGCCGTGG 
TCATTTCCTG GTTGGATAGG TCACGGTCAA AACTGGCCCT ATGCCCAACC CCAAACTACT 
GCTCTATATG TTGTGTCTTG GATATTGGGT GCTAAACAAC ATCACAATTT GGATATTGAT 
TATATTGGGA TTTGGAATGA GAGAACATTT GACAGTAAAT ATATAAAGGT ATTAAGAGAA 
GTGCTTTCTC AGAATGGCCT GAAAAGAGTG AAGATCATAG CCAGTGATTA CCTCTGGCTG 
CCCATTTCCA TTGCTATGCT ACATGATTAT GAACTCATGA AAGCTGTCGA TGTTATCGGG 
GCTCATTATC CTGGAACTCA TACTATACCT GAAGCACTGA AGACCCAAAA AATACTTTGG 
TCTTCAGAAG ATTACAGCAC TTTCAATGAT GAAGTGGGTG GAGGCTGCTG GGCCCGTATT 
TTGAATCAGA ACTATGTGAA TGGCAACATG ACTGCCACAA TTGCTTGGAA TTTGGTGGCT 
AGTTACTACC AGCAACTACC TTTTGGGAGA GATGGATTGA TGACTGCTCA GGAGCCTTGG 
AGTGGACATT ATGTTGTAGA GAGTCCTATC TGGATTTCAG CTCACACCAC TCAATTTGCT 
CAACCAGGAT GGCAATATCT AAAAACAGTT GGCCATTTAG TGAAAGGTGG AAGCTATGTT 
GCTCTTACTG ATGGCTTAGG TAACCTCACT ATTATCATTG AAACCATGAC TCATAAGCAT 
TCAAAATGCA TAAGGCCACC TCTTCCTTTC TTCAATGTGT CAGAACAAAT TGCAAATTTC 
TCTCTTAGTG GATCCTTTAA ACAATTAAGA ACGTTACAGG TGTGGTATTC CAAACTAGGG 
GACAAAACTG GAAATAACTC TATCTTATTC AAACAACTAC CTCCTCTAAA GATTAATGAT 
GGTACAGTCA CATTAAAATT GGGTCTGGAT GAAGTATATA CTTTAACAAC TATCACTACA 
GGTCAGAAAG GCAGTTATCC TATTCCTCCA GAGTCACAAC CATTTCCAAC AAGCTATAAA 
GATGATTTCA ACATTCGTAC TCCACCTTTT AGTGAAGCTC CGAATTTTGC TGATCAGACG 
GGAGTTTTTG AGTACTTCAC CAATCCCGAG CAAGGGGGGG ACCATGCCTT CACTTTACGC 
CAAGTGCTCA CTCAGCGACC TATCACCTGG GCTGCTGATG CAGATCAGAC CATCAGTGTT 
ATAGGGGATT ATAACTGGAA GGATCTGGTA ATAAGTTGTG ATATCTACAT AGAGACACCA 
CATAGTGGTG GTGTATTCAT TGCTGGAAGA GTAAATAAAG GTGGGGAAGT CATCCGAAGC 
GCTGCAGGAA TTTTCTTCTG GGTTTTTGCA GATGGGACCT ACCAAGTTAC AAATGATCTT 
GCTAGACTGT TGATATTAAA GAAAGGACAT GCTAATGTTA GTGCTGGGAA ATGGTACACC 
CTCACTCTGA ATATTAAAGA CTCTTCTGTT AATGTCATAC TAGATAATAC ACATCTCTGG 
GAAGGTGTGC TACCCTTATA TCCCAAGAAT GGTTGGGCAG CAATTGGAAC ACATAGCTTT 
GAGTTTGCAC AGTTTGACAA CTTTCATATA GAGGCTACAA ATGTGCATTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023351156.1
CDS	59..2212
Translation	MKTRQIGTGVPGAEGRSLLLLLLRLLSSLLLLSHAGASSLRYFDNYSLRPDPSYVLEDVAGLGRVFDGIGGLSGGGTSLDLFSFPFEIQASYEQATSRLLVNYPEPYRSEILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYANDENYFRGYEWWLMKEAKKRNPNIKLIGLPWSFPGWIGHGQNWPYAQPQTTALYVVSWILGAKQHHNLDIDYIGIWNERTFDSKYIKVLREVLSQNGLKRVKIIASDYLWLPISIAMLHDYELMKAVDVIGAHYPGTHTIPEALKTQKILWSSEDYSTFNDEVGGGCWARILNQNYVNGNMTATIAWNLVASYYQQLPFGRDGLMTAQEPWSGHYVVESPIWISAHTTQFAQPGWQYLKTVGHLVKGGSYVALTDGLGNLTIIIETMTHKHSKCIRPPLPFFNVSEQIANFSLSGSFKQLRTLQVWYSKLGDKTGNNSILFKQLPPLKINDGTVTLKLGLDEVYTLTTITTGQKGSYPIPPESQPFPTSYKDDFNIRTPPFSEAPNFADQTGVFEYFTNPEQGGDHAFTLRQVLTQRPITWAADADQTISVIGDYNWKDLVISCDIYIETPHSGGVFIAGRVNKGGEVIRSAAGIFFWVFADGTYQVTNDLARLLILKKGHANVSAGKWYTLTLNIKDSSVNVILDNTHLWEGVLPLYPKNGWAAIGTHSFEFAQFDNFHIEATNVHS

Target ORF information:

RefSeq Version	XM_023495388.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii galactosylceramidase (GALC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023495388.1

ORF Insert Sequence:

ATGAAGACGC GCCAGATTGG GACTGGCGTC CCGGGGGCGG AGGGTCGGTC ACTTCTGCTG 
TTGCTGCTGC GGCTGCTTTC CTCGCTGCTG CTTCTGAGCC ATGCGGGAGC TTCCAGCTTA 
CGATACTTTG ACAATTACAG TCTGCGCCCA GACCCCAGTT ACGTCCTGGA GGATGTCGCC 
GGCCTTGGTC GGGTGTTTGA CGGCATCGGC GGGCTGAGCG GCGGCGGGAC TTCCTTGGAT 
TTGTTTTCTT TCCCCTTTGA AATACAAGCT TCTTATGAGC AGGCAACCTC CCGACTTCTA 
GTCAATTATC CTGAGCCATA TCGTTCTGAA ATATTGGATT ATTTATTTAA GCCAAACTTT 
GGTGCATCTT TGCATATTCT GAAAGTGGAA ATAGGAGGTG ATGGACAAAC AACAGATGGC 
ACTGAACCCT CTCATATGCA TTATGCAAAT GATGAGAATT ATTTCCGGGG CTATGAATGG 
TGGCTAATGA AGGAAGCTAA GAAGAGGAAT CCCAATATTA AGCTTATTGG GTTGCCGTGG 
TCATTTCCTG GTTGGATAGG TCACGGTCAA AACTGGCCCT ATGCCCAACC CCAAACTACT 
GCTCTATATG TTGTGTCTTG GATATTGGGT GCTAAACAAC ATCACAATTT GGATATTGAT 
TATATTGGGA TTTGGAATGA GAGAACATTT GACAGTAAAT ATATAAAGGT ATTAAGAGAA 
GTGCTTTCTC AGAATGGCCT GAAAAGAGTG AAGATCATAG CCAGTGATTA CCTCTGGCTG 
CCCATTTCCA TTGCTATGCT ACATGATTAT GAACTCATGA AAGCTGTCGA TGTTATCGGG 
GCTCATTATC CTGGAACTCA TACTATACCT GAAGCACTGA AGACCCAAAA AATACTTTGG 
TCTTCAGAAG ATTACAGCAC TTTCAATGAT GAAGTGGGTG GAGGCTGCTG GGCCCGTATT 
TTGAATCAGA ACTATGTGAA TGGCAACATG ACTGCCACAA TTGCTTGGAA TTTGGTGGCT 
AGTTACTACC AGCAACTACC TTTTGGGAGA GATGGATTGA TGACTGCTCA GGAGCCTTGG 
AGTGGACATT ATGTTGTAGA GAGTCCTATC TGGATTTCAG CTCACACCAC TCAATTTGCT 
CAACCAGGAT GGCAATATCT AAAAACAGTT GGCCATTTAG TGAAAGGTGG AAGCTATGTT 
GCTCTTACTG ATGGCTTAGG TAACCTCACT ATTATCATTG AAACCATGAC TCATAAGCAT 
TCAAAATGCA TAAGGCCACC TCTTCCTTTC TTCAATGTGT CAGAACAAAT TGCAAATTTC 
TCTCTTAGTG GATCCTTTAA ACAATTAAGA ACGTTACAGG TGTGGTATTC CAAACTAGGG 
GACAAAACTG GAAATAACTC TATCTTATTC AAACAACTAC CTCCTCTAAA GATTAATGAT 
GGTACAGTCA CATTAAAATT GGGTCTGGAT GAAGTATATA CTTTAACAAC TATCACTACA 
GGTCAGAAAG GCAGTTATCC TATTCCTCCA GAGTCACAAC CATTTCCAAC AAGCTATAAA 
GATGATTTCA ACATTCGTAC TCCACCTTTT AGTGAAGCTC CGAATTTTGC TGATCAGACG 
GGAGTTTTTG AGTACTTCAC CAATCCCGAG CAAGGGGGGG ACCATGCCTT CACTTTACGC 
CAAGTGCTCA CTCAGCGACC TATCACCTGG GCTGCTGATG CAGATCAGAC CATCAGTGTT 
ATAGGGGATT ATAACTGGAA GGATCTGGTA ATAAGTTGTG ATATCTACAT AGAGACACCA 
CATAGTGGTG GTGTATTCAT TGCTGGAAGA GTAAATAAAG GTGGGGAAGT CATCCGAAGC 
GCTGCAGGAA TTTTCTTCTG GGTTTTTGCA GATGGGACCT ACCAAGTTAC AAATGATCTT 
GCTAGACTGT TGATATTAAA GAAAGGACAT GCTAATGTTA GTGCTGGGAA ATGGTACACC 
CTCACTCTGA ATATTAAAGA CTCTTCTGTT AATGTCATAC TAGATAATAC ACATCTCTGG 
GAAGGTGTGC TACCCTTATA TCCCAAGAAT GGTTGGGCAG CAATTGGAAC ACATAGCTTT 
GAGTTTGCAC AGTTTGACAA CTTTCATATA GAGGCTACAA ATGTGCATTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GALC ( XM_023495389.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023351157.1 Sarcophilus harrisii galactosylceramidase (GALC), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSa151188
Clone ID Related Accession (Same CDS sequence)	XM_023495389.1
Accession Version	XM_023495389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	galactocerebrosidase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTATTT TTGCACATGC AACCTCCCGA CTTCTAGTCA ATTATCCTGA GCCATATCGT 
TCTGAAATAT TGGATTATTT ATTTAAGCCA AACTTTGGTG CATCTTTGCA TATTCTGAAA 
GTGGAAATAG GAGGTGATGG ACAAACAACA GATGGCACTG AACCCTCTCA TATGCATTAT 
GCAAATGATG AGAATTATTT CCGGGGCTAT GAATGGTGGC TAATGAAGGA AGCTAAGAAG 
AGGAATCCCA ATATTAAGCT TATTGGGTTG CCGTGGTCAT TTCCTGGTTG GATAGGTCAC 
GGTCAAAACT GGCCCTATGC CCAACCCCAA ACTACTGCTC TATATGTTGT GTCTTGGATA 
TTGGGTGCTA AACAACATCA CAATTTGGAT ATTGATTATA TTGGGATTTG GAATGAGAGA 
ACATTTGACA GTAAATATAT AAAGGTATTA AGAGAAGTGC TTTCTCAGAA TGGCCTGAAA 
AGAGTGAAGA TCATAGCCAG TGATTACCTC TGGCTGCCCA TTTCCATTGC TATGCTACAT 
GATTATGAAC TCATGAAAGC TGTCGATGTT ATCGGGGCTC ATTATCCTGG AACTCATACT 
ATACCTGAAG CACTGAAGAC CCAAAAAATA CTTTGGTCTT CAGAAGATTA CAGCACTTTC 
AATGATGAAG TGGGTGGAGG CTGCTGGGCC CGTATTTTGA ATCAGAACTA TGTGAATGGC 
AACATGACTG CCACAATTGC TTGGAATTTG GTGGCTAGTT ACTACCAGCA ACTACCTTTT 
GGGAGAGATG GATTGATGAC TGCTCAGGAG CCTTGGAGTG GACATTATGT TGTAGAGAGT 
CCTATCTGGA TTTCAGCTCA CACCACTCAA TTTGCTCAAC CAGGATGGCA ATATCTAAAA 
ACAGTTGGCC ATTTAGTGAA AGGTGGAAGC TATGTTGCTC TTACTGATGG CTTAGGTAAC 
CTCACTATTA TCATTGAAAC CATGACTCAT AAGCATTCAA AATGCATAAG GCCACCTCTT 
CCTTTCTTCA ATGTGTCAGA ACAAATTGCA AATTTCTCTC TTAGTGGATC CTTTAAACAA 
TTAAGAACGT TACAGGTGTG GTATTCCAAA CTAGGGGACA AAACTGGAAA TAACTCTATC 
TTATTCAAAC AACTACCTCC TCTAAAGATT AATGATGGTA CAGTCACATT AAAATTGGGT 
CTGGATGAAG TATATACTTT AACAACTATC ACTACAGGTC AGAAAGGCAG TTATCCTATT 
CCTCCAGAGT CACAACCATT TCCAACAAGC TATAAAGATG ATTTCAACAT TCGTACTCCA 
CCTTTTAGTG AAGCTCCGAA TTTTGCTGAT CAGACGGGAG TTTTTGAGTA CTTCACCAAT 
CCCGAGCAAG GGGGGGACCA TGCCTTCACT TTACGCCAAG TGCTCACTCA GCGACCTATC 
ACCTGGGCTG CTGATGCAGA TCAGACCATC AGTGTTATAG GGGATTATAA CTGGAAGGAT 
CTGGTAATAA GTTGTGATAT CTACATAGAG ACACCACATA GTGGTGGTGT ATTCATTGCT 
GGAAGAGTAA ATAAAGGTGG GGAAGTCATC CGAAGCGCTG CAGGAATTTT CTTCTGGGTT 
TTTGCAGATG GGACCTACCA AGTTACAAAT GATCTTGCTA GACTGTTGAT ATTAAAGAAA 
GGACATGCTA ATGTTAGTGC TGGGAAATGG TACACCCTCA CTCTGAATAT TAAAGACTCT 
TCTGTTAATG TCATACTAGA TAATACACAT CTCTGGGAAG GTGTGCTACC CTTATATCCC 
AAGAATGGTT GGGCAGCAAT TGGAACACAT AGCTTTGAGT TTGCACAGTT TGACAACTTT 
CATATAGAGG CTACAAATGT GCATTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023351157.1
CDS	16..1905
Translation	MLIFAHATSRLLVNYPEPYRSEILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYANDENYFRGYEWWLMKEAKKRNPNIKLIGLPWSFPGWIGHGQNWPYAQPQTTALYVVSWILGAKQHHNLDIDYIGIWNERTFDSKYIKVLREVLSQNGLKRVKIIASDYLWLPISIAMLHDYELMKAVDVIGAHYPGTHTIPEALKTQKILWSSEDYSTFNDEVGGGCWARILNQNYVNGNMTATIAWNLVASYYQQLPFGRDGLMTAQEPWSGHYVVESPIWISAHTTQFAQPGWQYLKTVGHLVKGGSYVALTDGLGNLTIIIETMTHKHSKCIRPPLPFFNVSEQIANFSLSGSFKQLRTLQVWYSKLGDKTGNNSILFKQLPPLKINDGTVTLKLGLDEVYTLTTITTGQKGSYPIPPESQPFPTSYKDDFNIRTPPFSEAPNFADQTGVFEYFTNPEQGGDHAFTLRQVLTQRPITWAADADQTISVIGDYNWKDLVISCDIYIETPHSGGVFIAGRVNKGGEVIRSAAGIFFWVFADGTYQVTNDLARLLILKKGHANVSAGKWYTLTLNIKDSSVNVILDNTHLWEGVLPLYPKNGWAAIGTHSFEFAQFDNFHIEATNVHS

Target ORF information:

RefSeq Version	XM_023495389.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii galactosylceramidase (GALC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023495389.1

ORF Insert Sequence:

ATGCTTATTT TTGCACATGC AACCTCCCGA CTTCTAGTCA ATTATCCTGA GCCATATCGT 
TCTGAAATAT TGGATTATTT ATTTAAGCCA AACTTTGGTG CATCTTTGCA TATTCTGAAA 
GTGGAAATAG GAGGTGATGG ACAAACAACA GATGGCACTG AACCCTCTCA TATGCATTAT 
GCAAATGATG AGAATTATTT CCGGGGCTAT GAATGGTGGC TAATGAAGGA AGCTAAGAAG 
AGGAATCCCA ATATTAAGCT TATTGGGTTG CCGTGGTCAT TTCCTGGTTG GATAGGTCAC 
GGTCAAAACT GGCCCTATGC CCAACCCCAA ACTACTGCTC TATATGTTGT GTCTTGGATA 
TTGGGTGCTA AACAACATCA CAATTTGGAT ATTGATTATA TTGGGATTTG GAATGAGAGA 
ACATTTGACA GTAAATATAT AAAGGTATTA AGAGAAGTGC TTTCTCAGAA TGGCCTGAAA 
AGAGTGAAGA TCATAGCCAG TGATTACCTC TGGCTGCCCA TTTCCATTGC TATGCTACAT 
GATTATGAAC TCATGAAAGC TGTCGATGTT ATCGGGGCTC ATTATCCTGG AACTCATACT 
ATACCTGAAG CACTGAAGAC CCAAAAAATA CTTTGGTCTT CAGAAGATTA CAGCACTTTC 
AATGATGAAG TGGGTGGAGG CTGCTGGGCC CGTATTTTGA ATCAGAACTA TGTGAATGGC 
AACATGACTG CCACAATTGC TTGGAATTTG GTGGCTAGTT ACTACCAGCA ACTACCTTTT 
GGGAGAGATG GATTGATGAC TGCTCAGGAG CCTTGGAGTG GACATTATGT TGTAGAGAGT 
CCTATCTGGA TTTCAGCTCA CACCACTCAA TTTGCTCAAC CAGGATGGCA ATATCTAAAA 
ACAGTTGGCC ATTTAGTGAA AGGTGGAAGC TATGTTGCTC TTACTGATGG CTTAGGTAAC 
CTCACTATTA TCATTGAAAC CATGACTCAT AAGCATTCAA AATGCATAAG GCCACCTCTT 
CCTTTCTTCA ATGTGTCAGA ACAAATTGCA AATTTCTCTC TTAGTGGATC CTTTAAACAA 
TTAAGAACGT TACAGGTGTG GTATTCCAAA CTAGGGGACA AAACTGGAAA TAACTCTATC 
TTATTCAAAC AACTACCTCC TCTAAAGATT AATGATGGTA CAGTCACATT AAAATTGGGT 
CTGGATGAAG TATATACTTT AACAACTATC ACTACAGGTC AGAAAGGCAG TTATCCTATT 
CCTCCAGAGT CACAACCATT TCCAACAAGC TATAAAGATG ATTTCAACAT TCGTACTCCA 
CCTTTTAGTG AAGCTCCGAA TTTTGCTGAT CAGACGGGAG TTTTTGAGTA CTTCACCAAT 
CCCGAGCAAG GGGGGGACCA TGCCTTCACT TTACGCCAAG TGCTCACTCA GCGACCTATC 
ACCTGGGCTG CTGATGCAGA TCAGACCATC AGTGTTATAG GGGATTATAA CTGGAAGGAT 
CTGGTAATAA GTTGTGATAT CTACATAGAG ACACCACATA GTGGTGGTGT ATTCATTGCT 
GGAAGAGTAA ATAAAGGTGG GGAAGTCATC CGAAGCGCTG CAGGAATTTT CTTCTGGGTT 
TTTGCAGATG GGACCTACCA AGTTACAAAT GATCTTGCTA GACTGTTGAT ATTAAAGAAA 
GGACATGCTA ATGTTAGTGC TGGGAAATGG TACACCCTCA CTCTGAATAT TAAAGACTCT 
TCTGTTAATG TCATACTAGA TAATACACAT CTCTGGGAAG GTGTGCTACC CTTATATCCC 
AAGAATGGTT GGGCAGCAAT TGGAACACAT AGCTTTGAGT TTGCACAGTT TGACAACTTT 
CATATAGAGG CTACAAATGT GCATTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GALC ( XM_031952407.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031808267.1 Sarcophilus harrisii galactosylceramidase (GALC), transcript variant X2, mRNA.

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CloneID	OSa225108
Clone ID Related Accession (Same CDS sequence)	XM_031952407.1
Accession Version	XM_031952407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	galactocerebrosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTATG CAAATGATGA GAATTATTTC CGGGGCTATG AATGGTGGCT AATGAAGGAA 
GCTAAGAAGA GGAATCCCAA TATTAAGCTT ATTGGGTTGC CGTGGTCATT TCCTGGTTGG 
ATAGGTCACG GTCAAAACTG GCCCTATGCC CAACCCCAAA CTACTGCTCT ATATGTTGTG 
TCTTGGATAT TGGGTGCTAA ACAACATCAC AATTTGGATA TTGATTATAT TGGGATTTGG 
AATGAGAGAA CATTTGACAG TAAATATATA AAGGTATTAA GAGAAGTGCT TTCTCAGAAT 
GGCCTGAAAA GAGTGAAGAT CATAGCCAGT GATTACCTCT GGCTGCCCAT TTCCATTGCT 
ATGCTACATG ATTATGAACT CATGAAAGCT GTCGATGTTA TCGGGGCTCA TTATCCTGGA 
ACTCATACTA TACCTGAAGC ACTGAAGACC CAAAAAATAC TTTGGTCTTC AGAAGATTAC 
AGCACTTTCA ATGATGAAGT GGGTGGAGGC TGCTGGGCCC GTATTTTGAA TCAGAACTAT 
GTGAATGGCA ACATGACTGC CACAATTGCT TGGAATTTGG TGGCTAGTTA CTACCAGCAA 
CTACCTTTTG GGAGAGATGG ATTGATGACT GCTCAGGAGC CTTGGAGTGG ACATTATGTT 
GTAGAGAGTC CTATCTGGAT TTCAGCTCAC ACCACTCAAT TTGCTCAACC AGGATGGCAA 
TATCTAAAAA CAGTTGGCCA TTTAGTGAAA GGTGGAAGCT ATGTTGCTCT TACTGATGGC 
TTAGGTAACC TCACTATTAT CATTGAAACC ATGACTCATA AGCATTCAAA ATGCATAAGG 
CCACCTCTTC CTTTCTTCAA TGTGTCAGAA CAAATTGCAA ATTTCTCTCT TAGTGGATCC 
TTTAAACAAT TAAGAACGTT ACAGGTGTGG TATTCCAAAC TAGGGGACAA AACTGGAAAT 
AACTCTATCT TATTCAAACA ACTACCTCCT CTAAAGATTA ATGATGGTAC AGTCACATTA 
AAATTGGGTC TGGATGAAGT ATATACTTTA ACAACTATCA CTACAGGTCA GAAAGGCAGT 
TATCCTATTC CTCCAGAGTC ACAACCATTT CCAACAAGCT ATAAAGATGA TTTCAACATT 
CGTACTCCAC CTTTTAGTGA AGCTCCGAAT TTTGCTGATC AGACGGGAGT TTTTGAGTAC 
TTCACCAATC CCGAGCAAGG GGGGGACCAT GCCTTCACTT TACGCCAAGT GCTCACTCAG 
CGACCTATCA CCTGGGCTGC TGATGCAGAT CAGACCATCA GTGTTATAGG GGATTATAAC 
TGGAAGGATC TGGTAATAAG TTGTGATATC TACATAGAGA CACCACATAG TGGTGGTGTA 
TTCATTGCTG GAAGAGTAAA TAAAGGTGGG GAAGTCATCC GAAGCGCTGC AGGAATTTTC 
TTCTGGGTTT TTGCAGATGG GACCTACCAA GTTACAAATG ATCTTGCTAG ACTGTTGATA 
TTAAAGAAAG GACATGCTAA TGTTAGTGCT GGGAAATGGT ACACCCTCAC TCTGAATATT 
AAAGACTCTT CTGTTAATGT CATACTAGAT AATACACATC TCTGGGAAGG TGTGCTACCC 
TTATATCCCA AGAATGGTTG GGCAGCAATT GGAACACATA GCTTTGAGTT TGCACAGTTT 
GACAACTTTC ATATAGAGGC TACAAATGTG CATTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031808267.1
CDS	231..1949
Translation	MHYANDENYFRGYEWWLMKEAKKRNPNIKLIGLPWSFPGWIGHGQNWPYAQPQTTALYVVSWILGAKQHHNLDIDYIGIWNERTFDSKYIKVLREVLSQNGLKRVKIIASDYLWLPISIAMLHDYELMKAVDVIGAHYPGTHTIPEALKTQKILWSSEDYSTFNDEVGGGCWARILNQNYVNGNMTATIAWNLVASYYQQLPFGRDGLMTAQEPWSGHYVVESPIWISAHTTQFAQPGWQYLKTVGHLVKGGSYVALTDGLGNLTIIIETMTHKHSKCIRPPLPFFNVSEQIANFSLSGSFKQLRTLQVWYSKLGDKTGNNSILFKQLPPLKINDGTVTLKLGLDEVYTLTTITTGQKGSYPIPPESQPFPTSYKDDFNIRTPPFSEAPNFADQTGVFEYFTNPEQGGDHAFTLRQVLTQRPITWAADADQTISVIGDYNWKDLVISCDIYIETPHSGGVFIAGRVNKGGEVIRSAAGIFFWVFADGTYQVTNDLARLLILKKGHANVSAGKWYTLTLNIKDSSVNVILDNTHLWEGVLPLYPKNGWAAIGTHSFEFAQFDNFHIEATNVHS

Target ORF information:

RefSeq Version	XM_031952407.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii galactosylceramidase (GALC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031952407.1

ORF Insert Sequence:

ATGCATTATG CAAATGATGA GAATTATTTC CGGGGCTATG AATGGTGGCT AATGAAGGAA 
GCTAAGAAGA GGAATCCCAA TATTAAGCTT ATTGGGTTGC CGTGGTCATT TCCTGGTTGG 
ATAGGTCACG GTCAAAACTG GCCCTATGCC CAACCCCAAA CTACTGCTCT ATATGTTGTG 
TCTTGGATAT TGGGTGCTAA ACAACATCAC AATTTGGATA TTGATTATAT TGGGATTTGG 
AATGAGAGAA CATTTGACAG TAAATATATA AAGGTATTAA GAGAAGTGCT TTCTCAGAAT 
GGCCTGAAAA GAGTGAAGAT CATAGCCAGT GATTACCTCT GGCTGCCCAT TTCCATTGCT 
ATGCTACATG ATTATGAACT CATGAAAGCT GTCGATGTTA TCGGGGCTCA TTATCCTGGA 
ACTCATACTA TACCTGAAGC ACTGAAGACC CAAAAAATAC TTTGGTCTTC AGAAGATTAC 
AGCACTTTCA ATGATGAAGT GGGTGGAGGC TGCTGGGCCC GTATTTTGAA TCAGAACTAT 
GTGAATGGCA ACATGACTGC CACAATTGCT TGGAATTTGG TGGCTAGTTA CTACCAGCAA 
CTACCTTTTG GGAGAGATGG ATTGATGACT GCTCAGGAGC CTTGGAGTGG ACATTATGTT 
GTAGAGAGTC CTATCTGGAT TTCAGCTCAC ACCACTCAAT TTGCTCAACC AGGATGGCAA 
TATCTAAAAA CAGTTGGCCA TTTAGTGAAA GGTGGAAGCT ATGTTGCTCT TACTGATGGC 
TTAGGTAACC TCACTATTAT CATTGAAACC ATGACTCATA AGCATTCAAA ATGCATAAGG 
CCACCTCTTC CTTTCTTCAA TGTGTCAGAA CAAATTGCAA ATTTCTCTCT TAGTGGATCC 
TTTAAACAAT TAAGAACGTT ACAGGTGTGG TATTCCAAAC TAGGGGACAA AACTGGAAAT 
AACTCTATCT TATTCAAACA ACTACCTCCT CTAAAGATTA ATGATGGTAC AGTCACATTA 
AAATTGGGTC TGGATGAAGT ATATACTTTA ACAACTATCA CTACAGGTCA GAAAGGCAGT 
TATCCTATTC CTCCAGAGTC ACAACCATTT CCAACAAGCT ATAAAGATGA TTTCAACATT 
CGTACTCCAC CTTTTAGTGA AGCTCCGAAT TTTGCTGATC AGACGGGAGT TTTTGAGTAC 
TTCACCAATC CCGAGCAAGG GGGGGACCAT GCCTTCACTT TACGCCAAGT GCTCACTCAG 
CGACCTATCA CCTGGGCTGC TGATGCAGAT CAGACCATCA GTGTTATAGG GGATTATAAC 
TGGAAGGATC TGGTAATAAG TTGTGATATC TACATAGAGA CACCACATAG TGGTGGTGTA 
TTCATTGCTG GAAGAGTAAA TAAAGGTGGG GAAGTCATCC GAAGCGCTGC AGGAATTTTC 
TTCTGGGTTT TTGCAGATGG GACCTACCAA GTTACAAATG ATCTTGCTAG ACTGTTGATA 
TTAAAGAAAG GACATGCTAA TGTTAGTGCT GGGAAATGGT ACACCCTCAC TCTGAATATT 
AAAGACTCTT CTGTTAATGT CATACTAGAT AATACACATC TCTGGGAAGG TGTGCTACCC 
TTATATCCCA AGAATGGTTG GGCAGCAATT GGAACACATA GCTTTGAGTT TGCACAGTTT 
GACAACTTTC ATATAGAGGC TACAAATGTG CATTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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GALC cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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