PF3D7_0500100 cDNA ORF clone, Plasmodium falciparum 3D7

Gene
Clones
gRNAs

Summary

Gene Symbol	PF3D7_0500100
Entrez Gene ID	812899
Full Name	erythrocyte membrane protein 1, PfEMP1
Gene Type	protein-coding
Organism	Plasmodium falciparum 3D7

mRNA and Protein(s)

mRNA	Protein	Name
XM_001351528.1	XP_001351564.1	erythrocyte membrane protein 1, PfEMP1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.
Hall N, Pain A, Berriman M, Churcher C, Harris B, Harris D, Mungall K, Bowman S, Atkin R, Baker S, Barron A, Brooks K, Buckee CO, Burrows C, Cherevach I, Chillingworth C, Chillingworth T, Christodoulou Z, Clark L, Clark R, Corton C, Cronin A, Davies R, Davis P, Dear P, Dearden F, Doggett J, Feltwell T, Goble A, Goodhead I, Gwilliam R, Hamlin N, Hance Z, Harper D, Hauser H, Hornsby T, Holroyd S, Horrocks P, Humphray S, Jagels K, James KD, Johnson D, Kerhornou A, Knights A, Konfortov B, Kyes S, Larke N, Lawson D, Lennard N, Line A, Maddison M, McLean J, Mooney P, Moule S, Murphy L, Oliver K, Ormond D, Price C, Quail MA, Rabbinowitsch E, Rajandream MA, Rutter S, Rutherford KM, Sanders M, Simmonds M, Seeger K, Sharp S, Smith R, Squares R, Squares S, Stevens K, Taylor K, Tivey A, Unwin L, Whitehead S, Woodward J, Sulston JE, Craig A, Newbold C, Barrell BG
Nature419(6906)527-31(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PF3D7_0500100 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PF3D7_0500100 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPr02620	XM_001351528.1 Latest version!	Plasmodium falciparum 3D7 erythrocyte membrane protein 1, PfEMP1 (PF3D7_0500100), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PF3D7_0500100 ( XM_001351528.1 ) cDNA ORF clone, Plasmodium falciparum 3D7 -> XP_001351564.1 Plasmodium falciparum 3D7 erythrocyte membrane protein 1, PfEMP1 (PF3D7_0500100), partial mRNA.

Price & Availability↑

CloneID	OPr02620
Clone ID Related Accession (Same CDS sequence)	XM_001351528.1
Accession Version	XM_001351528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1522080000000
Organism	Plasmodium falciparum 3D7
Product	erythrocyte membrane protein 1, PfEMP1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_004326). COMPLETENESS: incomplete on both ends.

ATGGGGCCGC CAGGTATTAC TGGTACGCAG GGTGAAACTG CCAAACATAT GTTTGATAGG 
ATAGGGAAAC AAGTGTACGA AACAGTGAAA AATGAAGCTG AAAATTATAT TAGTGAATTG 
GAAGGAAAGT TGTCACAAGC AACACTTTTG GGGGAAAGAG TTAGCAGCCT TAAAACATGC 
CAACTTGTAG AGGATTATCG TAGTAAGGCT AATGGTGATG TTAAAAGGTA TCCGTGCGCT 
AATAGATCAC CAGTTCGTTT TTCCGATGAA AGTCGAAGTC AATGTACATA TAATAGAATA 
AAAGATAATG AAACCGATGA TAATGCATGT GGCGCCTGCG CGCCGTATCG ACGTTTACAT 
TTATGTGATT ATAATTTGGA AAAAATGGGC AAAACGTCGA CAACGAAGCA TGATTTGTTG 
GCAGAAGTAT GTATGGCAGC AAAATACGAA GGGGACTCAA TAAAAACACA TTATACAATA 
CATAAACACA CAAATAATGA TTCTGCTGCT GAATTATGTA CTGAGTTGGC ACGAAGTTTT 
GCAGATATTG GAGACATTAT ACGAGGAAAA GATCTGTATC TCGGTGATAT AAAAAAAAAG 
CAAAATGGAA AAAAAACAGA AAGAGAAAAA TTAGAAGAGA ATTTAAAAAG AATTTTCGGG 
AAAATACATG AGGACGTGAC GAATGGGAAG AAGGAGGTGC TAAAAACACG CTACAAAGAT 
ATTAATGATC CAGAATTTTT TAAATTACGA GAAGATTGGT GGACTGCGAA TCGCGCCACA 
GTATGGAAAG CCATCACATG TCATGCAGGA GAAAGTGATA AATATTTTCG AAACACATGC 
AATGATAGTG AACATAGTGG AACTTTTTCT CAACCTAATA AATACTGCCG GTGTAACGGC 
GACAAACCAG GTGAAGACAA GGCAAATGTC GATCCCCCAA CCTATTTTGA TTATGTGCCG 
CAGTATCTTC GCTGGTTCGA GGAATGGGCA GAAGATTTTT GTAGAATAAG GAAACATAAA 
TTAAAAAATG CTAAAGAACA ATGTCGTGAA AAATATAAAA GTGGTACGGA TCGATATTGT 
AGCCGTAATG GCTACGATTG CACACAAACT ATTAGAGGAA GAAACATTCT TGTCTCCGAT 
TCGGAATGTA CTAACTGCTC TGTTGTATGC ACTCCTTTTG TAAAATGGAT AGAGAACAAA 
AAACTGGAAT TTGAAAAACA AAAAGGAAAA TATACAAAAG AAATAGAAAA AGCAAACGGA 
ACATCAAACG GAACAACAAT AAGAACTCAA TATGGAACAA TTAATAATAT GTATAGAAAA 
GATTTTTATC AACAACTACA AAGTGGCTAT GGAGATGTCA ATGCTTTTTT AGAATTGTTA 
AATAAAGAAA CAACATGCAA AGATCACCCT AAAGTGGAAG AAAAATCTGA TATTGATTTC 
AATGAAGGTA CTGAAAAAAC ATTTTCTCAT ACGGAATATT GCGAAACCTG TCCGTGGTGT 
GCTACCAAAA AAAAAGGAAT TGATGGAAAC TGGGAAGAAC AAAAGTATGA AGAAGGATGC 
GAAAACTATT TAATGAAACC CATTGATGAA AGTAAAAGTA CTGATATTGA TTTGCTTGTC 
AAGGATACGT CAGGAACAAC TATGGTGGAG AAACTAGGAG GTTTGTGTAA CGATTCTTCT 
AAAAGAACTG TTCAAATGGA AAATTGGCAA TGTCATTATG AAAAAAAAAG TCAATATGAA 
GATGGTTTTG ATAAGGATTA TTGTGTACTA AAAGATGACA AAAAAAAAAA ACCAGAACAT 
AGGACAATTA AATCCTATTA TACCTTATTT CCAAATTGGA TAAATGAAAT GTTAAAAGAT 
TCGATAGATT GGAGTAAAGA ACTTAAAACC TGTATAAATA ATGAAAAACC AACTAATTGT 
ATACGTGAGT GTAAAAGTAA ATGCGATTGT TTTAAAAAAT GGGTTGTACA AAAAGAACAA 
GAATGGAAAC AATTAGAAGA ACACTATGAA AAAGAAAATT TTAGTGGAGA TTTTGGTCCA 
AGAATTAGTC CTTATGTGAC TCTTGAAGGG AATTTACAAT ACAGTTATTT GGAAATGATT 
CGAAAGTATT ATGCACAAGA AAAACCCGTA CAAGAAATTG AACAAATAAT TGAAAAAAAT 
AAGAACAATT TCGAAGTTAA AGAAGATGAT AATTCAATAA CCAAGTTCTT ACAGCAAGAA 
AAAGGAATTG CCACCAAATG CATAGAAAAA CAGGAAGAAT GCAAGCAACA AAAAAAACAA 
CAACGACAAA AACAACCCGC CGATAAAGTT GTCTCCCGCT CCGGCGCCTC ACCCGACACC 
GCCTCACCCG ACACTAAGGC CACTGAGGAA AAGGAAGAAG AAGAAGAGGA AGAAGAAGAA 
GAGGACCTCG GCGAGGAGTC GGAGGAGCCG GAGGACCAAG CGGTGGTAGA CCAGGATGGG 
CAGGGGGAGA CCACAGAAAA GAAGGTACCG GCGACCACAG AAGAGGGATC ACCAAAAGAA 
ACAACAACAC CAGAGAAGTC AGTAGACGTT TGCAAAACGG TGGCCGAACT ATTTAGTAAC 
GTGGACAATC TGAAAGAAGC CTGCACACAA AAATATGGCG GAAATAACTC ACGTTTAGGT 
TGGAAGTGCA TACCAACTAG TGGTGGTGAA AAAGCCACAG GTGGTAGTGG TGAATCCACT 
GGTAGTGATG CCACCACCGG TGGTAGTATT TGTGTGCCAC CGCGAAGACG ACGATTATAT 
GTCACACCAC TAACGACATG GGCGAGTGGT GGTACCACAC AGGTGGAGAC ACAGGCGAGT 
GGTGGTAACA CGGAGACGTC ACAAGTTAGT GGTGAGACAA CACCACAAGG TCAAACACCA 
TCAGAAAGTG AGGCCCAAAC TGCGTCACAA GATCCTAGTG AAAAACTACG TACTGCATTT 
ATTGAATCTG CTGCTGTAGA AACTTTTTTT TTATGGGATA GGTACAAAAA GGAAAACACG 
AAGAGACAAA GTGTGTTACC ACTACTAGAA CCCATCAACG GTGATACTAT CAGTGATGAC 
AATAACCCCG AAAAACTGTT AAAAGTTGGT AAGATACCCC CCGATTTTTT GAGATTAATG 
TTTTATACAT TAGGTGATTA TAGAGATATA TTATTTAGTA GTAGTAACAC AAGTGACACA 
ACTGGTAAAG AGACACCTAG TAGTAGTAAT GACAACCTCA AAAATATTGT GATTGAAGCT 
GGTGGTACTG ACGAGAAGGA CAAGCAGAAA ATGAAAGAAA TACAACAGAA AATAGATAAA 
ATTCTCAAAC AAAGTGGCAC AACTCCTCCC ACACCTGTCA CCCACTCCCC ATCTAGTGGT 
ACAACCCCCT CCTCCTGGTG GGAAAATAAT GCAAAACATA TATGGAAAGG AATGGTTTGT 
GCACTAACAT ACAAAGACAA CACGAATGGT GGACCACCAA CACAGGATAA TGATTTGAAA 
AAGGCACTTT GGGACGACAA AACGAACGAG CCAAAAAAAA GCGAATACAA ATACGACCAA 
GTGGAACTTA AAGAAGAAAA TAGTGGTACT GGCGTCACCA AAGGCCACGC TGAAGCCCCT 
GGTGATAACA CTCCCCTGTC CAAATTTGTG TTACGCCCCC CTTATTTCCG TTACCTTGAA 
GAATGGGGTG AAACATTTTG TAGGGAACGG ACGAGGAGGT TGGGGAAGAT AAAGGGGGAG 
TGTAAGGTGG ATGACAGTGG AAGTGGTCGT GGTGGTGTTA TAAAACGACA ATATAGTGGG 
GATGGGGAAG ATTGTGACAA GGTTCATGAG GATCCTACTA CACTTCCGGA TTTAGGTTCC 
AGTTGTCCCA AATCTTGTAG TTCTTATAGA AAATGGATAA AAATAAAAAA AGACGAATAT 
GAGAAACAAC AAAAGGCATA TGAACAACAA AAAAAAAACT GCGAAAAGGA AAGTGAGGGT 
GTTGAAAATA AATCTGATAT TTGTGATCAA AAATTTCTTC AAAATCTTAA AAACTATGGG 
TCAATTGATT CATTTTTAGA TAGATTAAAA AAAGGACCAT GTTCTAAAAC TTATAATGAG 
AGTGGAGAGG ATAATAATAA AATATTTGAG GATACAGAGA AAACGTTTGG ACATCAAAAT 
TATTGTGGTC CATGTTCTGA ATTTAAAATA AATTGTGAAA AAGGTAATTG CGATAAGACT 
AAAGGACAGG AGTGCAATGG TAAAAAGACT ATTGATGCAC AAAATTTTGA ACAAATGGGA 
CAGACTGCTA AAGAATTTGT TATGCGTGTC AGTGATAATG ATACAACGAA ATTTGAAGGT 
CATGGTTTAG AGGATTGTAA CGGTGCAGGT ATCTTTAAAG GTATTAAAGA AAATAAATGG 
AAATGTGATA AGGTATGTGG TTATGTTGTA TGTAAACCGA AAAACGGTAA TGGAAAAAAT 
GAAGGTACAT ACATTATACA AATTAGAGCA TTGGTTAGAC GTTGGGTAGA ATATTTTTTA 
GAAGATTATA ATAAAATTAG AAAAAAATTA AAGCCATGTA TAAATGATGG TAAAGAACCT 
AAATGTATAA AAACTTGTGA TAAAAAATGT AATTGTGCTG GAAAATGGAT AAAGCTGAAA 
CAAGATGAAT GGGAAAATAT AAAAAATCGT TTCAATGAGC AATATAAAAA TGGCAATGAA 
GGTGATTACC CAGTGAAAAC TATTTTGGAG GAATTGATAT CCCGAATTGC TGCTGCAACT 
GATAAAGCAG AACGTGGGAG TTTAGATAAA TTAAAGACGT CCCTTGGATG TAATTGCTCT 
GATAACTCAC AAAAAAAAAA TACTGACGAA AGAGATATCG TAGAATGTTT GCTTGAAAAG 
CTTCAAGTAA AAGCAACATC TTGTCAATCC CAACATAGTG ACAAAACAGA ACAACAGTGT 
CAAGAATACA CCCCTGAACC CGATGAAGAG GACTTACTCC TTGAAGAAGA AAATGAAAAC 
CAAGTGGAAA ATCAAAAAGT GGGAAATAAA GCGCCAGCAT TTTGTCCACC TCCAGAAAAA 
CCACCGAAAG AGGAACCAGA TGAAAAGTGT GGAGATAAAG AAGAAGAAAA AGAACAAGAG 
GAGGAAAAAG ACAAAGGAAA TGAAGAACAA AGTGGCGCCC CTTCATCACC ATCACCTAGT 
GAAGGCACAA AGGAACTTCC ACCTCCACCT GAACCCCCAG AGGAAGCCAA ACCCCCGGAA 
CCAGTCAAAC CAACACCAGC ACCGGCCCCT GCGGCGCCTC CAGCAGCACC AGCACAACCA 
ACCCTCCCTG CGGACGAGCC ATTCAATCGT GACATCCTGG CAACGACCAT CCCTTTTGGT 
ATTGCTTTCG CTTTAGGATC TATTGCTTTT TTATTTTTGA AGAAAAAAAC CAAATCTACT 
ATTGATCTTT TGCGTGTTAT TAATATCCCC AAAAGTGATT ATGATATACC GACAAAACTT 
TCACCCAATA GATATATACC TTATACTAGT GGTAAATACA GAGGCAAACG GTACATTTAC 
CTTGAAGGAG ATAGTGGAAC TGATAGTGGT TACACCGATC ATTATAGTGA TATTACTTCA 
TCTTCCGAAA GTGAGTATGA AGAGATGGAT ATTAATGATA TATATGTACC TGGTAGTCCT 
AAATATAAAA CGTTGATAGA AGTGGTACTT GAACCTAGTG GTAACAACAC AACAGCTAGT 
GGTAACAACA CAACAGCTAG TGATACACAA AATGATATAC AAAATGATGG TATACCTAGT 
AATAAATTTA GTGATAATGA GTGGAATACA TTGAAAGATG ATTTTATATC ACAATATCTA 
CAAAGTGAAC AACCAAATGA TTTACCAAAT GATTATAAAA GTGGAAATGT TACTTTAAAT 
ACAGAACCTA ATATTTTATA TTTTGATAAA CCTGAAGAAA AACCTTTTAT TACTTCTATT 
CATGATAGAA ATTTATATAC TGGAGAAGAA TATAGTTATA ATATTAATAT GAGTACTAAT 
AGTATGGATG ATATTCCAAT TAATCGTGAT AATAATGTTT ATAGTGGTAT AGATTTAATT 
AATGACGCAC TAAATGGTGA TTATGACATT TACGATGAAA TGTTGAAACG AAAAGAAAAT 
GAATTATTTG GGACAAATCA TGTGAAACAA ACAAGTATAC ATAGTGTTGC AAAAAATACA 
AATAGTGACC CCATACACAA CCAACTGGAA CTATTCCATA AATGGTTAGA TCGACATAGA 
GATATGTGCG AACAGTGGGA AAATCATCAC GAACGATTAG CCAAATTGAA AGAAGAGTGG 
GAAAATGAGA CACATAGTGG TAACACTCAC CCTAGTGATA GTAACAAAAC GTTAAATACT 
GATGTTTCTA TACAGATAGA TATGGATGAT GGAAAACCAA AGAAGGAATT TACTAATATG 
GATACTATCT TGGAAGATCT GGATAAATAT AATGAACCTT ATTATGATGT GCAAGATGAT 
ATTTATTATG ATGTAAATGA TCATGATACA TCAACTGTAA ATCCTAATAA TATGGAAAAA 
CCTAGTAAGG TAAAGATTGA ATTAGATGTA AATAAAAAGA CGATAAAAGA GAAATATCCT 
ATATCAGATG TGTGGGATAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001351564.1
CDS	1..6624
Translation	MGPPGITGTQGETAKHMFDRIGKQVYETVKNEAENYISELEGKLSQATLLGERVSSLKTCQLVEDYRSKANGDVKRYPCANRSPVRFSDESRSQCTYNRIKDNETDDNACGACAPYRRLHLCDYNLEKMGKTSTTKHDLLAEVCMAAKYEGDSIKTHYTIHKHTNNDSAAELCTELARSFADIGDIIRGKDLYLGDIKKKQNGKKTEREKLEENLKRIFGKIHEDVTNGKKEVLKTRYKDINDPEFFKLREDWWTANRATVWKAITCHAGESDKYFRNTCNDSEHSGTFSQPNKYCRCNGDKPGEDKANVDPPTYFDYVPQYLRWFEEWAEDFCRIRKHKLKNAKEQCREKYKSGTDRYCSRNGYDCTQTIRGRNILVSDSECTNCSVVCTPFVKWIENKKLEFEKQKGKYTKEIEKANGTSNGTTIRTQYGTINNMYRKDFYQQLQSGYGDVNAFLELLNKETTCKDHPKVEEKSDIDFNEGTEKTFSHTEYCETCPWCATKKKGIDGNWEEQKYEEGCENYLMKPIDESKSTDIDLLVKDTSGTTMVEKLGGLCNDSSKRTVQMENWQCHYEKKSQYEDGFDKDYCVLKDDKKKKPEHRTIKSYYTLFPNWINEMLKDSIDWSKELKTCINNEKPTNCIRECKSKCDCFKKWVVQKEQEWKQLEEHYEKENFSGDFGPRISPYVTLEGNLQYSYLEMIRKYYAQEKPVQEIEQIIEKNKNNFEVKEDDNSITKFLQQEKGIATKCIEKQEECKQQKKQQRQKQPADKVVSRSGASPDTASPDTKATEEKEEEEEEEEEEDLGEESEEPEDQAVVDQDGQGETTEKKVPATTEEGSPKETTTPEKSVDVCKTVAELFSNVDNLKEACTQKYGGNNSRLGWKCIPTSGGEKATGGSGESTGSDATTGGSICVPPRRRRLYVTPLTTWASGGTTQVETQASGGNTETSQVSGETTPQGQTPSESEAQTASQDPSEKLRTAFIESAAVETFFLWDRYKKENTKRQSVLPLLEPINGDTISDDNNPEKLLKVGKIPPDFLRLMFYTLGDYRDILFSSSNTSDTTGKETPSSSNDNLKNIVIEAGGTDEKDKQKMKEIQQKIDKILKQSGTTPPTPVTHSPSSGTTPSSWWENNAKHIWKGMVCALTYKDNTNGGPPTQDNDLKKALWDDKTNEPKKSEYKYDQVELKEENSGTGVTKGHAEAPGDNTPLSKFVLRPPYFRYLEEWGETFCRERTRRLGKIKGECKVDDSGSGRGGVIKRQYSGDGEDCDKVHEDPTTLPDLGSSCPKSCSSYRKWIKIKKDEYEKQQKAYEQQKKNCEKESEGVENKSDICDQKFLQNLKNYGSIDSFLDRLKKGPCSKTYNESGEDNNKIFEDTEKTFGHQNYCGPCSEFKINCEKGNCDKTKGQECNGKKTIDAQNFEQMGQTAKEFVMRVSDNDTTKFEGHGLEDCNGAGIFKGIKENKWKCDKVCGYVVCKPKNGNGKNEGTYIIQIRALVRRWVEYFLEDYNKIRKKLKPCINDGKEPKCIKTCDKKCNCAGKWIKLKQDEWENIKNRFNEQYKNGNEGDYPVKTILEELISRIAAATDKAERGSLDKLKTSLGCNCSDNSQKKNTDERDIVECLLEKLQVKATSCQSQHSDKTEQQCQEYTPEPDEEDLLLEEENENQVENQKVGNKAPAFCPPPEKPPKEEPDEKCGDKEEEKEQEEEKDKGNEEQSGAPSSPSPSEGTKELPPPPEPPEEAKPPEPVKPTPAPAPAAPPAAPAQPTLPADEPFNRDILATTIPFGIAFALGSIAFLFLKKKTKSTIDLLRVINIPKSDYDIPTKLSPNRYIPYTSGKYRGKRYIYLEGDSGTDSGYTDHYSDITSSSESEYEEMDINDIYVPGSPKYKTLIEVVLEPSGNNTTASGNNTTASDTQNDIQNDGIPSNKFSDNEWNTLKDDFISQYLQSEQPNDLPNDYKSGNVTLNTEPNILYFDKPEEKPFITSIHDRNLYTGEEYSYNINMSTNSMDDIPINRDNNVYSGIDLINDALNGDYDIYDEMLKRKENELFGTNHVKQTSIHSVAKNTNSDPIHNQLELFHKWLDRHRDMCEQWENHHERLAKLKEEWENETHSGNTHPSDSNKTLNTDVSIQIDMDDGKPKKEFTNMDTILEDLDKYNEPYYDVQDDIYYDVNDHDTSTVNPNNMEKPSKVKIELDVNKKTIKEKYPISDVWDI

Target ORF information:

RefSeq Version	XM_001351528.1
Organism	Plasmodium falciparum 3D7
Definition	Plasmodium falciparum 3D7 erythrocyte membrane protein 1, PfEMP1 (PF3D7_0500100), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001351528.1

ORF Insert Sequence:

ATGGGGCCGC CAGGTATTAC TGGTACGCAG GGTGAAACTG CCAAACATAT GTTTGATAGG 
ATAGGGAAAC AAGTGTACGA AACAGTGAAA AATGAAGCTG AAAATTATAT TAGTGAATTG 
GAAGGAAAGT TGTCACAAGC AACACTTTTG GGGGAAAGAG TTAGCAGCCT TAAAACATGC 
CAACTTGTAG AGGATTATCG TAGTAAGGCT AATGGTGATG TTAAAAGGTA TCCGTGCGCT 
AATAGATCAC CAGTTCGTTT TTCCGATGAA AGTCGAAGTC AATGTACATA TAATAGAATA 
AAAGATAATG AAACCGATGA TAATGCATGT GGCGCCTGCG CGCCGTATCG ACGTTTACAT 
TTATGTGATT ATAATTTGGA AAAAATGGGC AAAACGTCGA CAACGAAGCA TGATTTGTTG 
GCAGAAGTAT GTATGGCAGC AAAATACGAA GGGGACTCAA TAAAAACACA TTATACAATA 
CATAAACACA CAAATAATGA TTCTGCTGCT GAATTATGTA CTGAGTTGGC ACGAAGTTTT 
GCAGATATTG GAGACATTAT ACGAGGAAAA GATCTGTATC TCGGTGATAT AAAAAAAAAG 
CAAAATGGAA AAAAAACAGA AAGAGAAAAA TTAGAAGAGA ATTTAAAAAG AATTTTCGGG 
AAAATACATG AGGACGTGAC GAATGGGAAG AAGGAGGTGC TAAAAACACG CTACAAAGAT 
ATTAATGATC CAGAATTTTT TAAATTACGA GAAGATTGGT GGACTGCGAA TCGCGCCACA 
GTATGGAAAG CCATCACATG TCATGCAGGA GAAAGTGATA AATATTTTCG AAACACATGC 
AATGATAGTG AACATAGTGG AACTTTTTCT CAACCTAATA AATACTGCCG GTGTAACGGC 
GACAAACCAG GTGAAGACAA GGCAAATGTC GATCCCCCAA CCTATTTTGA TTATGTGCCG 
CAGTATCTTC GCTGGTTCGA GGAATGGGCA GAAGATTTTT GTAGAATAAG GAAACATAAA 
TTAAAAAATG CTAAAGAACA ATGTCGTGAA AAATATAAAA GTGGTACGGA TCGATATTGT 
AGCCGTAATG GCTACGATTG CACACAAACT ATTAGAGGAA GAAACATTCT TGTCTCCGAT 
TCGGAATGTA CTAACTGCTC TGTTGTATGC ACTCCTTTTG TAAAATGGAT AGAGAACAAA 
AAACTGGAAT TTGAAAAACA AAAAGGAAAA TATACAAAAG AAATAGAAAA AGCAAACGGA 
ACATCAAACG GAACAACAAT AAGAACTCAA TATGGAACAA TTAATAATAT GTATAGAAAA 
GATTTTTATC AACAACTACA AAGTGGCTAT GGAGATGTCA ATGCTTTTTT AGAATTGTTA 
AATAAAGAAA CAACATGCAA AGATCACCCT AAAGTGGAAG AAAAATCTGA TATTGATTTC 
AATGAAGGTA CTGAAAAAAC ATTTTCTCAT ACGGAATATT GCGAAACCTG TCCGTGGTGT 
GCTACCAAAA AAAAAGGAAT TGATGGAAAC TGGGAAGAAC AAAAGTATGA AGAAGGATGC 
GAAAACTATT TAATGAAACC CATTGATGAA AGTAAAAGTA CTGATATTGA TTTGCTTGTC 
AAGGATACGT CAGGAACAAC TATGGTGGAG AAACTAGGAG GTTTGTGTAA CGATTCTTCT 
AAAAGAACTG TTCAAATGGA AAATTGGCAA TGTCATTATG AAAAAAAAAG TCAATATGAA 
GATGGTTTTG ATAAGGATTA TTGTGTACTA AAAGATGACA AAAAAAAAAA ACCAGAACAT 
AGGACAATTA AATCCTATTA TACCTTATTT CCAAATTGGA TAAATGAAAT GTTAAAAGAT 
TCGATAGATT GGAGTAAAGA ACTTAAAACC TGTATAAATA ATGAAAAACC AACTAATTGT 
ATACGTGAGT GTAAAAGTAA ATGCGATTGT TTTAAAAAAT GGGTTGTACA AAAAGAACAA 
GAATGGAAAC AATTAGAAGA ACACTATGAA AAAGAAAATT TTAGTGGAGA TTTTGGTCCA 
AGAATTAGTC CTTATGTGAC TCTTGAAGGG AATTTACAAT ACAGTTATTT GGAAATGATT 
CGAAAGTATT ATGCACAAGA AAAACCCGTA CAAGAAATTG AACAAATAAT TGAAAAAAAT 
AAGAACAATT TCGAAGTTAA AGAAGATGAT AATTCAATAA CCAAGTTCTT ACAGCAAGAA 
AAAGGAATTG CCACCAAATG CATAGAAAAA CAGGAAGAAT GCAAGCAACA AAAAAAACAA 
CAACGACAAA AACAACCCGC CGATAAAGTT GTCTCCCGCT CCGGCGCCTC ACCCGACACC 
GCCTCACCCG ACACTAAGGC CACTGAGGAA AAGGAAGAAG AAGAAGAGGA AGAAGAAGAA 
GAGGACCTCG GCGAGGAGTC GGAGGAGCCG GAGGACCAAG CGGTGGTAGA CCAGGATGGG 
CAGGGGGAGA CCACAGAAAA GAAGGTACCG GCGACCACAG AAGAGGGATC ACCAAAAGAA 
ACAACAACAC CAGAGAAGTC AGTAGACGTT TGCAAAACGG TGGCCGAACT ATTTAGTAAC 
GTGGACAATC TGAAAGAAGC CTGCACACAA AAATATGGCG GAAATAACTC ACGTTTAGGT 
TGGAAGTGCA TACCAACTAG TGGTGGTGAA AAAGCCACAG GTGGTAGTGG TGAATCCACT 
GGTAGTGATG CCACCACCGG TGGTAGTATT TGTGTGCCAC CGCGAAGACG ACGATTATAT 
GTCACACCAC TAACGACATG GGCGAGTGGT GGTACCACAC AGGTGGAGAC ACAGGCGAGT 
GGTGGTAACA CGGAGACGTC ACAAGTTAGT GGTGAGACAA CACCACAAGG TCAAACACCA 
TCAGAAAGTG AGGCCCAAAC TGCGTCACAA GATCCTAGTG AAAAACTACG TACTGCATTT 
ATTGAATCTG CTGCTGTAGA AACTTTTTTT TTATGGGATA GGTACAAAAA GGAAAACACG 
AAGAGACAAA GTGTGTTACC ACTACTAGAA CCCATCAACG GTGATACTAT CAGTGATGAC 
AATAACCCCG AAAAACTGTT AAAAGTTGGT AAGATACCCC CCGATTTTTT GAGATTAATG 
TTTTATACAT TAGGTGATTA TAGAGATATA TTATTTAGTA GTAGTAACAC AAGTGACACA 
ACTGGTAAAG AGACACCTAG TAGTAGTAAT GACAACCTCA AAAATATTGT GATTGAAGCT 
GGTGGTACTG ACGAGAAGGA CAAGCAGAAA ATGAAAGAAA TACAACAGAA AATAGATAAA 
ATTCTCAAAC AAAGTGGCAC AACTCCTCCC ACACCTGTCA CCCACTCCCC ATCTAGTGGT 
ACAACCCCCT CCTCCTGGTG GGAAAATAAT GCAAAACATA TATGGAAAGG AATGGTTTGT 
GCACTAACAT ACAAAGACAA CACGAATGGT GGACCACCAA CACAGGATAA TGATTTGAAA 
AAGGCACTTT GGGACGACAA AACGAACGAG CCAAAAAAAA GCGAATACAA ATACGACCAA 
GTGGAACTTA AAGAAGAAAA TAGTGGTACT GGCGTCACCA AAGGCCACGC TGAAGCCCCT 
GGTGATAACA CTCCCCTGTC CAAATTTGTG TTACGCCCCC CTTATTTCCG TTACCTTGAA 
GAATGGGGTG AAACATTTTG TAGGGAACGG ACGAGGAGGT TGGGGAAGAT AAAGGGGGAG 
TGTAAGGTGG ATGACAGTGG AAGTGGTCGT GGTGGTGTTA TAAAACGACA ATATAGTGGG 
GATGGGGAAG ATTGTGACAA GGTTCATGAG GATCCTACTA CACTTCCGGA TTTAGGTTCC 
AGTTGTCCCA AATCTTGTAG TTCTTATAGA AAATGGATAA AAATAAAAAA AGACGAATAT 
GAGAAACAAC AAAAGGCATA TGAACAACAA AAAAAAAACT GCGAAAAGGA AAGTGAGGGT 
GTTGAAAATA AATCTGATAT TTGTGATCAA AAATTTCTTC AAAATCTTAA AAACTATGGG 
TCAATTGATT CATTTTTAGA TAGATTAAAA AAAGGACCAT GTTCTAAAAC TTATAATGAG 
AGTGGAGAGG ATAATAATAA AATATTTGAG GATACAGAGA AAACGTTTGG ACATCAAAAT 
TATTGTGGTC CATGTTCTGA ATTTAAAATA AATTGTGAAA AAGGTAATTG CGATAAGACT 
AAAGGACAGG AGTGCAATGG TAAAAAGACT ATTGATGCAC AAAATTTTGA ACAAATGGGA 
CAGACTGCTA AAGAATTTGT TATGCGTGTC AGTGATAATG ATACAACGAA ATTTGAAGGT 
CATGGTTTAG AGGATTGTAA CGGTGCAGGT ATCTTTAAAG GTATTAAAGA AAATAAATGG 
AAATGTGATA AGGTATGTGG TTATGTTGTA TGTAAACCGA AAAACGGTAA TGGAAAAAAT 
GAAGGTACAT ACATTATACA AATTAGAGCA TTGGTTAGAC GTTGGGTAGA ATATTTTTTA 
GAAGATTATA ATAAAATTAG AAAAAAATTA AAGCCATGTA TAAATGATGG TAAAGAACCT 
AAATGTATAA AAACTTGTGA TAAAAAATGT AATTGTGCTG GAAAATGGAT AAAGCTGAAA 
CAAGATGAAT GGGAAAATAT AAAAAATCGT TTCAATGAGC AATATAAAAA TGGCAATGAA 
GGTGATTACC CAGTGAAAAC TATTTTGGAG GAATTGATAT CCCGAATTGC TGCTGCAACT 
GATAAAGCAG AACGTGGGAG TTTAGATAAA TTAAAGACGT CCCTTGGATG TAATTGCTCT 
GATAACTCAC AAAAAAAAAA TACTGACGAA AGAGATATCG TAGAATGTTT GCTTGAAAAG 
CTTCAAGTAA AAGCAACATC TTGTCAATCC CAACATAGTG ACAAAACAGA ACAACAGTGT 
CAAGAATACA CCCCTGAACC CGATGAAGAG GACTTACTCC TTGAAGAAGA AAATGAAAAC 
CAAGTGGAAA ATCAAAAAGT GGGAAATAAA GCGCCAGCAT TTTGTCCACC TCCAGAAAAA 
CCACCGAAAG AGGAACCAGA TGAAAAGTGT GGAGATAAAG AAGAAGAAAA AGAACAAGAG 
GAGGAAAAAG ACAAAGGAAA TGAAGAACAA AGTGGCGCCC CTTCATCACC ATCACCTAGT 
GAAGGCACAA AGGAACTTCC ACCTCCACCT GAACCCCCAG AGGAAGCCAA ACCCCCGGAA 
CCAGTCAAAC CAACACCAGC ACCGGCCCCT GCGGCGCCTC CAGCAGCACC AGCACAACCA 
ACCCTCCCTG CGGACGAGCC ATTCAATCGT GACATCCTGG CAACGACCAT CCCTTTTGGT 
ATTGCTTTCG CTTTAGGATC TATTGCTTTT TTATTTTTGA AGAAAAAAAC CAAATCTACT 
ATTGATCTTT TGCGTGTTAT TAATATCCCC AAAAGTGATT ATGATATACC GACAAAACTT 
TCACCCAATA GATATATACC TTATACTAGT GGTAAATACA GAGGCAAACG GTACATTTAC 
CTTGAAGGAG ATAGTGGAAC TGATAGTGGT TACACCGATC ATTATAGTGA TATTACTTCA 
TCTTCCGAAA GTGAGTATGA AGAGATGGAT ATTAATGATA TATATGTACC TGGTAGTCCT 
AAATATAAAA CGTTGATAGA AGTGGTACTT GAACCTAGTG GTAACAACAC AACAGCTAGT 
GGTAACAACA CAACAGCTAG TGATACACAA AATGATATAC AAAATGATGG TATACCTAGT 
AATAAATTTA GTGATAATGA GTGGAATACA TTGAAAGATG ATTTTATATC ACAATATCTA 
CAAAGTGAAC AACCAAATGA TTTACCAAAT GATTATAAAA GTGGAAATGT TACTTTAAAT 
ACAGAACCTA ATATTTTATA TTTTGATAAA CCTGAAGAAA AACCTTTTAT TACTTCTATT 
CATGATAGAA ATTTATATAC TGGAGAAGAA TATAGTTATA ATATTAATAT GAGTACTAAT 
AGTATGGATG ATATTCCAAT TAATCGTGAT AATAATGTTT ATAGTGGTAT AGATTTAATT 
AATGACGCAC TAAATGGTGA TTATGACATT TACGATGAAA TGTTGAAACG AAAAGAAAAT 
GAATTATTTG GGACAAATCA TGTGAAACAA ACAAGTATAC ATAGTGTTGC AAAAAATACA 
AATAGTGACC CCATACACAA CCAACTGGAA CTATTCCATA AATGGTTAGA TCGACATAGA 
GATATGTGCG AACAGTGGGA AAATCATCAC GAACGATTAG CCAAATTGAA AGAAGAGTGG 
GAAAATGAGA CACATAGTGG TAACACTCAC CCTAGTGATA GTAACAAAAC GTTAAATACT 
GATGTTTCTA TACAGATAGA TATGGATGAT GGAAAACCAA AGAAGGAATT TACTAATATG 
GATACTATCT TGGAAGATCT GGATAAATAT AATGAACCTT ATTATGATGT GCAAGATGAT 
ATTTATTATG ATGTAAATGA TCATGATACA TCAACTGTAA ATCCTAATAA TATGGAAAAA 
CCTAGTAAGG TAAAGATTGA ATTAGATGTA AATAAAAAGA CGATAAAAGA GAAATATCCT 
ATATCAGATG TGTGGGATAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.
Nature419(6906)527-31(2002 Oct)
Hall N,Pain A,Berriman M,Churcher C,Harris B,Harris D,Mungall K,Bowman S,Atkin R,Baker S,Barron A,Brooks K,Buckee CO,Burrows C,Cherevach I,Chillingworth C,Chillingworth T,Christodoulou Z,Clark L,Clark R,Corton C,Cronin A,Davies R,Davis P,Dear P,Dearden F,Doggett J,Feltwell T,Goble A,Goodhead I,Gwilliam R,Hamlin N,Hance Z,Harper D,Hauser H,Hornsby T,Holroyd S,Horrocks P,Humphray S,Jagels K,James KD,Johnson D,Kerhornou A,Knights A,Konfortov B,Kyes S,Larke N,Lawson D,Lennard N,Line A,Maddison M,McLean J,Mooney P,Moule S,Murphy L,Oliver K,Ormond D,Price C,Quail MA,Rabbinowitsch E,Rajandream MA,Rutter S,Rutherford KM,Sanders M,Simmonds M,Seeger K,Sharp S,Smith R,Squares R,Squares S,Stevens K,Taylor K,Tivey A,Unwin L,Whitehead S,Woodward J,Sulston JE,Craig A,Newbold C,Barrell BG

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PF3D7_0500100 cDNA ORF clone, Plasmodium falciparum 3D7

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