ATG2B cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATG2B
Entrez Gene ID	101121043
Full Name	autophagy related 2B
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012099021.2	XP_011954411.2	autophagy-related protein 2 homolog B isoform X2
XM_012112893.2	XP_011968283.1	autophagy-related protein 2 homolog B isoform X3
XM_012112892.1	XP_011968282.1	autophagy-related protein 2 homolog B isoform X2
XM_012099021.1	XP_011954411.1	autophagy-related protein 2 homolog B isoform X2
XM_004017983.2	XP_004018032.1	autophagy-related protein 2 homolog B isoform X1
XM_004017983.3	XP_004018032.2	autophagy-related protein 2 homolog B isoform X1
XM_012112891.1	XP_011968281.1	autophagy-related protein 2 homolog B isoform X1
XM_012099022.1	XP_011954412.1	autophagy-related protein 2 homolog B isoform X3

Pathways from BioSystems
Gene Ontology

The following ATG2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATG2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv02813	XM_012112893.2 Latest version!	Ovis aries musimon autophagy related 2B (ATG2B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm42092	XM_012099022.1 Latest version!	Ovis aries autophagy related 2B (ATG2B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv02811	XM_012112891.1 Latest version!	Ovis aries musimon autophagy related 2B (ATG2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOv02812	XM_012112892.1 Latest version!	Ovis aries musimon autophagy related 2B (ATG2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm14334	XM_004017983.2 Latest version!	Ovis aries autophagy related 2B (ATG2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm42091	XM_012099021.1 Latest version!	Ovis aries autophagy related 2B (ATG2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm68068	XM_004017983.3 Latest version!	Ovis aries autophagy related 2B (ATG2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSm68069	XM_012099021.2 Latest version!	Ovis aries autophagy related 2B (ATG2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATG2B ( XM_012112893.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011968283.1 Ovis aries musimon autophagy related 2B (ATG2B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOv02813
Clone ID Related Accession (Same CDS sequence)	XM_012112893.2
Accession Version	XM_012112893.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	autophagy-related protein 2 homolog B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942251.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012112893.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTGGT CAAGCTTCAT GACCAGCAGT ATGCAACTGG CAAAAGAATG TCTTAGCCAG 
AAACTAACAG ATGAACAAGG AGAAGGATCT CAGCCTTTTG AAGGACTTGA AAAATTTGCT 
GAAACCATCG AAACAGTACT GAGAAGAGTA AAAGTCACTT TTATAGATAC TGTCTTGAGA 
ATTGAACATG TGCCAGAAAA TTCCAAAACT GGAACTGCAC TTGAAATTCG AATAGAAAGA 
ACCATGTACT GTGATGAAAC TGCTGACGAG TCCTCAGGAA TTAATGTACA TCAACCCACA 
GCTTTTGCTC ACAAGTTACT TCAGCTCTCT GGAGTGTCTC TCTTCTGGGA TGAGTTTTCT 
GCATCTGCAA AATCCTCCCC AGTGTGTTCA ACTGCGCCAG TGGAAACAGA GCCAAAGCTG 
TCACCTAGCT GGAATCCAAA AATTGTTTAT GAGCCTCACC CACAACTAAC AAGGAATTTG 
CCAGAAGTAG CACCTTCTGA CCCAGTGCAG ATAGGAGGGC TCAATGGTAG GCTGGAGTTG 
AGTCTCACAT TGAAACAGAA TGAAGTACTT CCTGGAGCTA AGTTGGATAT TGATGGACAG 
ATAGACTCTA TACATCTATT CCTGTCACCC AGGCAGGTGC ACTTGCTTTT GGACATGTTA 
GCAGCTATTG CTGGACCAGA AAATTCAAGC AAAATTGGAT TAGCTAATAA AGATAGAAAA 
AATCGACCTA TGCAGCAAGA AGATGAATAT CGAATTCAAA TGGAATTGAA CCGATATTAT 
TTGAGGAAAG ATTCCCTCTC TGCCGGCGTG TCCTCAGAGC AAAGCTTTTA TGAGACAGAA 
ACGGCTCGTA CACCTTCTAG CCGGGAAGAA GAAGTTTTCT TCTCCATGGC TGATATGGAC 
ATGTCGCACA GTCTCTCTTC TCTTCCACCC CTTGGGGAAC CCCCAAATAT GGACCTTGAG 
TTATCATTAA CTAGTACATA TACAAACACC CCAGCAGGAT CTCCATTAAG TACTACTGTG 
CTTCAGCCAA CATGGGGAGA CTTCCTTGAT CATCATAAAG AACAGCCAGT AAGAGGATCA 
GCGTTACCCT CCAACCTAGT TCACCCAGCA TCTTTACAAA AGACTGCTCT TCCCTCTAGG 
TCTGTTTCAG TGGATGAATC CAGGCCTGAA CTTATTTTTA GAGTAGCTGT GGGAACGTTT 
TCAGTCTCTG TGCTTCACAT TGATCCTTTA TCTCCACCTG AATCATCACT GAATGTTAAT 
CCATTGACAC CAATGGCGAT AGCTTTCTTT ACTTGTATAG AAAAAATTGA TCCAGCACAA 
TTTTCAACAG AAGATTTTAA GTCTTTTCGA GCAGTATTTG CAGAAGCTTG CTCACATGAT 
CACCTTAGAT TTATAGGCAC TGGCATCAAA GTGTCCTATG AACAAAGACA AAGATCAGCT 
TCCCGATATT TCAGTACTGA TATGTCCATT GGGCAAATGG AACTTTTGGA ATGCTTGTTT 
CCGACTGATT CTCATTCTGT TCCTCCTCAC TATACAGAGC TTTTGATGTT CCATTCCAAA 
GAACGAACTG ATTCTCACCT CCCTGTGTGT CTTCAGCTTC ATTATAAGCA TTCTGAGAAT 
AAAGGACCTC AGGGTAGTCA AGCAAGACTT AGTTCCGTTC CTCAGAAGGC AGAATTACAG 
GTTAAGTTAA ATCCAGTGTT TTGTGAGCTG GATATCAGTA TTGTGGACAG GTTAAATTCC 
TTGCTTCAAC CGCAGAAACT CACTACAGTG GAGATGATGG CATCACATAT GTATACTTCA 
TATAATAAGC ATATTAGTTT GCACAAAGCT TTCACTGAAG TATTTCTAGA TGACTCACAG 
AATCCTGCAA ACTGCCGGGT ATCAGTACAA GTTGCCACAC CAGCATTAAA CCTTTCTGTT 
CGCTTCCCAA TACCTGATCT TCGATCAGAT CAAGAGAGAG GACCATGGTT TAAGAAGTCA 
CTTCAGAAGG AGATTCTTCA TTTAGCATTC ACAGATCTAG AGTTTAAAAC TGAATTCCTA 
GGAGGATCAA CCCCTGAACA AGTTAAATTG GAGCTTACAT TTAGAGAACT AGTTGGGTCA 
TTCCAGGAAG ATAAAGGAGA ACCATCCATT AAGTTTTTCC ATGTGGCTAG TGGCGGAGAT 
GGAGATACAA CGTCATCAGA TGACTTTGAC TGGCCGCGAA TTGTACTGAA AGTGAACCCA 
CCAGCCATGC ATTCCATTTT GGAGAGAATA GCAGCTGAGG AGGAAGAGGA AAGTGATGGC 
CACTATCAGG AGGAAGAGGA GGGAGGAGCT CATTCCCTGA AGGATGTCTG TGATCTGAGA 
AGGCCAGCCC CGTCTCCCTT CTCTTCCCGT AGAGTAATGT TTGAAAATGA ACAGATGGTG 
ATGCCAGGAG ACCCTGTAGA AATGACCGAG TTTCAGAATA AAGCAATCAG CAATTCTCAT 
TATGTGCTGG AACTTACATT ACCCAATATT CATATAACAC TGCCTAATAA AAGCTTTTAT 
GAGAAGCTTT ATAATAGGAT CTTTAATGAT TTGCTGCTAT GGGAACCAAC AGCTCCTTCA 
CCAGTAGAGA CATTTGAAAA TATTTCGTAT GGTATTGGGC TTTCAGTAGC CAGTCAGCTG 
ATTAATACCT TCAGCAAGGA TAGTTTTAGT CCATTTAAAT CTACAGTTCA CTATGATGAG 
GAAAGTGGAT CTGAGGAGGA GACTTTGCAG TATTTTTCTA CTGTTGATCC CAATTATCGT 
TCTCGTAGGA AAAAAAAACT AGACTCCCAG AACAAGAACT CACAGAGTTT TCTCTCAGTT 
CTTCTGAGTA TTAATCATGG GTTAATTGCA GTGTTTACAG ATGTAAAGCA AGATAATGGA 
GATCTGTTGG AAAACAAGCA TGGTGAATTC TGGTTCGAGT TCAGCAGCGG CTCGTTGTTT 
TGTGTGACAA AGTATGAAGG CTTTGACGAC AAGCACTACG TGTGCCTTCA CTGCAGCAGC 
TTCAGTCTCT ACCACAAAGG CATAGTGGAT GGAGCAGTTC CTTCTACAGA AACACGACTG 
CCTAGTTCAA CCCGCCCACA TTGGTTAGAA CCTACTATTT ATTCCTCTGA AGAAGATGGC 
CTCAGTCGAG CTTCTTCAGA TGGTGTTGGA GGAGACACTT TGAATATGCT GTCTGTTGCA 
GTTAAAATAG TGTCTGATAA ATCAGAGTCC AATACAAAGG AGTTTCTCAT TGCTGTAGGG 
CTAAAAGGAG CTACTCTCCA GCATAGAATG CTTCCTTCTG GGCTTAGCTG GCATGAGCAG 
ATTTTATACT TCTTGAATAT TGCTGATGAA CCTGTTCTGG GATACAATCC TCCAACTTCA 
TTTACAACCT TTCATGTTCA TCTCTGGAGC TGTGCGCTTG ATTATAGGCC TCTTTATTTG 
CCAATCCGAT CCCTTCTTAC TGTTGAAACA TTCAGCGTTT CAAGTAGTGT CGCCTTGGAT 
AAATCATCTT CAACTCTCAG AATAATTTTG GATGAAGCTG CTTTACACCT GTCTGACAAG 
TGTAATACTG TTACTATAAA TTTGAATAGA GATTATGTTC GTGTGATGGA TATGGGACTT 
TTGGAATTAA CCATAACTGC AGTAAAGTCT GATTCCGATG GAGAGCAAAC TGAACCACAC 
TTTGAATTGC ACTGTTCGAG TGACGTTGTC CATGTTAGAA CGTGCTCAGA CTCTTGTGCT 
GCTCTAATGA ATCTAATTCA ATACATTGCA AGCTATGGGG ACTTACATGC CACTAACAAG 
GCAGACGTGA AGCCAGGAGC TCCTCAAAGA AAGGTGAAGG TAGATTCTGT TGGTCGGTCA 
TCCTCACGTG GTCCAGTACT TCCTGAAGCA GATCAACAGA TACTACGAGA TTTGATGAGT 
GATGCTATGG AGGAGATTGA CATGCAAGAA GCAGCTTCAT CTGTAAAACC ACAGTCTAAT 
GGTGTTTCCG ATGAAAAATC TCAAGTTCAG GAACCATGTT GTTCAGACCT CTTCCTGTTT 
CCTGATGAGA GTGGAAACGT GTCCCAGGAG CCTGGCCCCA CTTACGCCTC ATTCACCCAC 
CACTTGATTA GTGATGCAGT GACAGGCGTG CCCACGGAAA ATGACGACTT TTGCATTCTT 
TTTGCACCAA GAGCAACTGT TCAGGAGAAG GAAGAAGAAC CAGTTGTAAA AATAATGGTT 
GATGATGCAA TTGTGATAAA AGAGAATTAT TTTAGTCTGC CTGTTACGAA GACAGACACT 
AGCAAAGCCC CGTTACACTT TCCCGTTCCT GTCGTTCGGT ACCTGGTTAA AGAAGTCTCT 
CTCGTCTGGC ATCTTTATGG AGGAAAAGAT TTTGGAACAG CCCCTCCTAC TTCTCCAGCT 
AAAAGTTACA TTAGTCCCCA TAGCTCACCT TCTCACACAC CTACAAGACA TGGACATAAT 
ATGGTATGTG GGGGAAAAGG AAGAAACCAT GACTTTTTAA TGGAAATACA GTTAAGCAAG 
GTAAAGTTTC AGCATGAGGT CTACCCGCCC TGCAAACCAG AATGCGAGTC TAGCCTTGTG 
GAGCACCCGG TGTCCCGGCA GGTGTTCATC GTGCAGGATC TTGAAATTCG AGATCGTCTG 
GCAACATCAC AAATGAATAA ATTTTTGTAC CTGTATTGCA GTAAAGAAAT GCCTCGGAAA 
GCTCATTCCA ACATGTTGAC AATTAAAGCA TTACACGTGC GTCCAGAGTC TGGCAGGTCC 
CCACAGGAGT GCTGCCTAAG AGTATCACTA ATGCCACTTC GCCTCAATAT TGACCAGGAT 
GCCTTGTTCT TCTTGAAGGA TTTCTTCACA AGTCTTTCTG CAGAAGTAGA GCTGCTCATG 
ACTCCAGATC CAGAAGTTAA AAAGTCTCCT GGGGCTGATG TCACCTGCAG TTTGCCAAGG 
CATTTAAGCA CCTCAAAAGA GCCAAATCTA GTTCTTTCTT TCCCTGGGCC AAAGCAGCCT 
TCCCCAAATG GCAGTGCCAA TTCAGTGCAA GCGGTTAATG GGGAAGAAGA GAAGGACTTC 
TCTCCTGAAG AAGCGTTGTT TAGTGATCAG CCCGTGTTTT TTAGAGAATT TAGATTCACA 
TCAGAAGTTC CTATTCGACT TGATTATCAT GGGAAACATG TATCAATGGA TCAGGGTACA 
TTAGCTGGGA TTTTGATTGG TCTCGCCCAG TTAAACTGCT CTGAACTAAA GCTGAAGAGG 
CTTTTCTATC GGCATGGTTT GCTAGGTGTG GACAAATTAT TCTCATATGC AATCACCGAA 
TGGCTTACTG ACATTAAGAA GAACCAGCTT CCAGGAATCC TGGGAGGTGT TGGGCCTATG 
CATTCACTAG TACAACTAGT GCAAGGCCTC AAGGACTTGG TCTGGTTACC AATTGAGCAG 
TACCGAAAGG ACGGTCGCAT TGTCAGAGGA TTTCAGAGAG GGGCTGCCTC CTTTGGCACC 
TCGACGGCGA TGGCTGCTCT CGAGCTCACA AACAGAATGG TTCAAACCAT ACAGGCAGCG 
GCAGAGACTG CGTATGACAT GGTGTCTCCA GGCACCCTTT CTATCGAGCC CAAGAAGGTC 
AGAAGGTTCC CCCATCACCG GTTAGCCCAC CAGCCAGTGG ACCTGAGGGA AGGGGTGGCC 
AAGGCCTACA GTGTCGTGAA GGAGGGAATC GCAGACACGG CTCAGACCAT TTATGAAACT 
GCCGCTCGGG AACACGAGAG CCGGGGGGTG ACGGGCGCCG TGGGGGAAGT CCTGCGCCAG 
CTCCCCCCGG CCGTGGTGAA ACCTCTGATC GTCGCCACAG AAGCGACATC GAACGTCCTG 
GGCGGCATGA GAAACCAGAT CAGACCAGAT GTTCGGCAGG ACGAGTCGCA GAAGTGGCGC 
CATGGGGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011968283.1
CDS	352..6246
Translation	MYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSVSVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQGSQARLSSVPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSQNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLIAVFTDVKQDNGDLLENKHGEFWFEFSSGSLFCVTKYEGFDDKHYVCLHCSSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVKVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDAIVIKENYFSLPVTKTDTSKAPLHFPVPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEVELLMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_012112893.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon autophagy related 2B (ATG2B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012112893.2

ORF Insert Sequence:

ATGTACTGGT CAAGCTTCAT GACCAGCAGT ATGCAACTGG CAAAAGAATG TCTTAGCCAG 
AAACTAACAG ATGAACAAGG AGAAGGATCT CAGCCTTTTG AAGGACTTGA AAAATTTGCT 
GAAACCATCG AAACAGTACT GAGAAGAGTA AAAGTCACTT TTATAGATAC TGTCTTGAGA 
ATTGAACATG TGCCAGAAAA TTCCAAAACT GGAACTGCAC TTGAAATTCG AATAGAAAGA 
ACCATGTACT GTGATGAAAC TGCTGACGAG TCCTCAGGAA TTAATGTACA TCAACCCACA 
GCTTTTGCTC ACAAGTTACT TCAGCTCTCT GGAGTGTCTC TCTTCTGGGA TGAGTTTTCT 
GCATCTGCAA AATCCTCCCC AGTGTGTTCA ACTGCGCCAG TGGAAACAGA GCCAAAGCTG 
TCACCTAGCT GGAATCCAAA AATTGTTTAT GAGCCTCACC CACAACTAAC AAGGAATTTG 
CCAGAAGTAG CACCTTCTGA CCCAGTGCAG ATAGGAGGGC TCAATGGTAG GCTGGAGTTG 
AGTCTCACAT TGAAACAGAA TGAAGTACTT CCTGGAGCTA AGTTGGATAT TGATGGACAG 
ATAGACTCTA TACATCTATT CCTGTCACCC AGGCAGGTGC ACTTGCTTTT GGACATGTTA 
GCAGCTATTG CTGGACCAGA AAATTCAAGC AAAATTGGAT TAGCTAATAA AGATAGAAAA 
AATCGACCTA TGCAGCAAGA AGATGAATAT CGAATTCAAA TGGAATTGAA CCGATATTAT 
TTGAGGAAAG ATTCCCTCTC TGCCGGCGTG TCCTCAGAGC AAAGCTTTTA TGAGACAGAA 
ACGGCTCGTA CACCTTCTAG CCGGGAAGAA GAAGTTTTCT TCTCCATGGC TGATATGGAC 
ATGTCGCACA GTCTCTCTTC TCTTCCACCC CTTGGGGAAC CCCCAAATAT GGACCTTGAG 
TTATCATTAA CTAGTACATA TACAAACACC CCAGCAGGAT CTCCATTAAG TACTACTGTG 
CTTCAGCCAA CATGGGGAGA CTTCCTTGAT CATCATAAAG AACAGCCAGT AAGAGGATCA 
GCGTTACCCT CCAACCTAGT TCACCCAGCA TCTTTACAAA AGACTGCTCT TCCCTCTAGG 
TCTGTTTCAG TGGATGAATC CAGGCCTGAA CTTATTTTTA GAGTAGCTGT GGGAACGTTT 
TCAGTCTCTG TGCTTCACAT TGATCCTTTA TCTCCACCTG AATCATCACT GAATGTTAAT 
CCATTGACAC CAATGGCGAT AGCTTTCTTT ACTTGTATAG AAAAAATTGA TCCAGCACAA 
TTTTCAACAG AAGATTTTAA GTCTTTTCGA GCAGTATTTG CAGAAGCTTG CTCACATGAT 
CACCTTAGAT TTATAGGCAC TGGCATCAAA GTGTCCTATG AACAAAGACA AAGATCAGCT 
TCCCGATATT TCAGTACTGA TATGTCCATT GGGCAAATGG AACTTTTGGA ATGCTTGTTT 
CCGACTGATT CTCATTCTGT TCCTCCTCAC TATACAGAGC TTTTGATGTT CCATTCCAAA 
GAACGAACTG ATTCTCACCT CCCTGTGTGT CTTCAGCTTC ATTATAAGCA TTCTGAGAAT 
AAAGGACCTC AGGGTAGTCA AGCAAGACTT AGTTCCGTTC CTCAGAAGGC AGAATTACAG 
GTTAAGTTAA ATCCAGTGTT TTGTGAGCTG GATATCAGTA TTGTGGACAG GTTAAATTCC 
TTGCTTCAAC CGCAGAAACT CACTACAGTG GAGATGATGG CATCACATAT GTATACTTCA 
TATAATAAGC ATATTAGTTT GCACAAAGCT TTCACTGAAG TATTTCTAGA TGACTCACAG 
AATCCTGCAA ACTGCCGGGT ATCAGTACAA GTTGCCACAC CAGCATTAAA CCTTTCTGTT 
CGCTTCCCAA TACCTGATCT TCGATCAGAT CAAGAGAGAG GACCATGGTT TAAGAAGTCA 
CTTCAGAAGG AGATTCTTCA TTTAGCATTC ACAGATCTAG AGTTTAAAAC TGAATTCCTA 
GGAGGATCAA CCCCTGAACA AGTTAAATTG GAGCTTACAT TTAGAGAACT AGTTGGGTCA 
TTCCAGGAAG ATAAAGGAGA ACCATCCATT AAGTTTTTCC ATGTGGCTAG TGGCGGAGAT 
GGAGATACAA CGTCATCAGA TGACTTTGAC TGGCCGCGAA TTGTACTGAA AGTGAACCCA 
CCAGCCATGC ATTCCATTTT GGAGAGAATA GCAGCTGAGG AGGAAGAGGA AAGTGATGGC 
CACTATCAGG AGGAAGAGGA GGGAGGAGCT CATTCCCTGA AGGATGTCTG TGATCTGAGA 
AGGCCAGCCC CGTCTCCCTT CTCTTCCCGT AGAGTAATGT TTGAAAATGA ACAGATGGTG 
ATGCCAGGAG ACCCTGTAGA AATGACCGAG TTTCAGAATA AAGCAATCAG CAATTCTCAT 
TATGTGCTGG AACTTACATT ACCCAATATT CATATAACAC TGCCTAATAA AAGCTTTTAT 
GAGAAGCTTT ATAATAGGAT CTTTAATGAT TTGCTGCTAT GGGAACCAAC AGCTCCTTCA 
CCAGTAGAGA CATTTGAAAA TATTTCGTAT GGTATTGGGC TTTCAGTAGC CAGTCAGCTG 
ATTAATACCT TCAGCAAGGA TAGTTTTAGT CCATTTAAAT CTACAGTTCA CTATGATGAG 
GAAAGTGGAT CTGAGGAGGA GACTTTGCAG TATTTTTCTA CTGTTGATCC CAATTATCGT 
TCTCGTAGGA AAAAAAAACT AGACTCCCAG AACAAGAACT CACAGAGTTT TCTCTCAGTT 
CTTCTGAGTA TTAATCATGG GTTAATTGCA GTGTTTACAG ATGTAAAGCA AGATAATGGA 
GATCTGTTGG AAAACAAGCA TGGTGAATTC TGGTTCGAGT TCAGCAGCGG CTCGTTGTTT 
TGTGTGACAA AGTATGAAGG CTTTGACGAC AAGCACTACG TGTGCCTTCA CTGCAGCAGC 
TTCAGTCTCT ACCACAAAGG CATAGTGGAT GGAGCAGTTC CTTCTACAGA AACACGACTG 
CCTAGTTCAA CCCGCCCACA TTGGTTAGAA CCTACTATTT ATTCCTCTGA AGAAGATGGC 
CTCAGTCGAG CTTCTTCAGA TGGTGTTGGA GGAGACACTT TGAATATGCT GTCTGTTGCA 
GTTAAAATAG TGTCTGATAA ATCAGAGTCC AATACAAAGG AGTTTCTCAT TGCTGTAGGG 
CTAAAAGGAG CTACTCTCCA GCATAGAATG CTTCCTTCTG GGCTTAGCTG GCATGAGCAG 
ATTTTATACT TCTTGAATAT TGCTGATGAA CCTGTTCTGG GATACAATCC TCCAACTTCA 
TTTACAACCT TTCATGTTCA TCTCTGGAGC TGTGCGCTTG ATTATAGGCC TCTTTATTTG 
CCAATCCGAT CCCTTCTTAC TGTTGAAACA TTCAGCGTTT CAAGTAGTGT CGCCTTGGAT 
AAATCATCTT CAACTCTCAG AATAATTTTG GATGAAGCTG CTTTACACCT GTCTGACAAG 
TGTAATACTG TTACTATAAA TTTGAATAGA GATTATGTTC GTGTGATGGA TATGGGACTT 
TTGGAATTAA CCATAACTGC AGTAAAGTCT GATTCCGATG GAGAGCAAAC TGAACCACAC 
TTTGAATTGC ACTGTTCGAG TGACGTTGTC CATGTTAGAA CGTGCTCAGA CTCTTGTGCT 
GCTCTAATGA ATCTAATTCA ATACATTGCA AGCTATGGGG ACTTACATGC CACTAACAAG 
GCAGACGTGA AGCCAGGAGC TCCTCAAAGA AAGGTGAAGG TAGATTCTGT TGGTCGGTCA 
TCCTCACGTG GTCCAGTACT TCCTGAAGCA GATCAACAGA TACTACGAGA TTTGATGAGT 
GATGCTATGG AGGAGATTGA CATGCAAGAA GCAGCTTCAT CTGTAAAACC ACAGTCTAAT 
GGTGTTTCCG ATGAAAAATC TCAAGTTCAG GAACCATGTT GTTCAGACCT CTTCCTGTTT 
CCTGATGAGA GTGGAAACGT GTCCCAGGAG CCTGGCCCCA CTTACGCCTC ATTCACCCAC 
CACTTGATTA GTGATGCAGT GACAGGCGTG CCCACGGAAA ATGACGACTT TTGCATTCTT 
TTTGCACCAA GAGCAACTGT TCAGGAGAAG GAAGAAGAAC CAGTTGTAAA AATAATGGTT 
GATGATGCAA TTGTGATAAA AGAGAATTAT TTTAGTCTGC CTGTTACGAA GACAGACACT 
AGCAAAGCCC CGTTACACTT TCCCGTTCCT GTCGTTCGGT ACCTGGTTAA AGAAGTCTCT 
CTCGTCTGGC ATCTTTATGG AGGAAAAGAT TTTGGAACAG CCCCTCCTAC TTCTCCAGCT 
AAAAGTTACA TTAGTCCCCA TAGCTCACCT TCTCACACAC CTACAAGACA TGGACATAAT 
ATGGTATGTG GGGGAAAAGG AAGAAACCAT GACTTTTTAA TGGAAATACA GTTAAGCAAG 
GTAAAGTTTC AGCATGAGGT CTACCCGCCC TGCAAACCAG AATGCGAGTC TAGCCTTGTG 
GAGCACCCGG TGTCCCGGCA GGTGTTCATC GTGCAGGATC TTGAAATTCG AGATCGTCTG 
GCAACATCAC AAATGAATAA ATTTTTGTAC CTGTATTGCA GTAAAGAAAT GCCTCGGAAA 
GCTCATTCCA ACATGTTGAC AATTAAAGCA TTACACGTGC GTCCAGAGTC TGGCAGGTCC 
CCACAGGAGT GCTGCCTAAG AGTATCACTA ATGCCACTTC GCCTCAATAT TGACCAGGAT 
GCCTTGTTCT TCTTGAAGGA TTTCTTCACA AGTCTTTCTG CAGAAGTAGA GCTGCTCATG 
ACTCCAGATC CAGAAGTTAA AAAGTCTCCT GGGGCTGATG TCACCTGCAG TTTGCCAAGG 
CATTTAAGCA CCTCAAAAGA GCCAAATCTA GTTCTTTCTT TCCCTGGGCC AAAGCAGCCT 
TCCCCAAATG GCAGTGCCAA TTCAGTGCAA GCGGTTAATG GGGAAGAAGA GAAGGACTTC 
TCTCCTGAAG AAGCGTTGTT TAGTGATCAG CCCGTGTTTT TTAGAGAATT TAGATTCACA 
TCAGAAGTTC CTATTCGACT TGATTATCAT GGGAAACATG TATCAATGGA TCAGGGTACA 
TTAGCTGGGA TTTTGATTGG TCTCGCCCAG TTAAACTGCT CTGAACTAAA GCTGAAGAGG 
CTTTTCTATC GGCATGGTTT GCTAGGTGTG GACAAATTAT TCTCATATGC AATCACCGAA 
TGGCTTACTG ACATTAAGAA GAACCAGCTT CCAGGAATCC TGGGAGGTGT TGGGCCTATG 
CATTCACTAG TACAACTAGT GCAAGGCCTC AAGGACTTGG TCTGGTTACC AATTGAGCAG 
TACCGAAAGG ACGGTCGCAT TGTCAGAGGA TTTCAGAGAG GGGCTGCCTC CTTTGGCACC 
TCGACGGCGA TGGCTGCTCT CGAGCTCACA AACAGAATGG TTCAAACCAT ACAGGCAGCG 
GCAGAGACTG CGTATGACAT GGTGTCTCCA GGCACCCTTT CTATCGAGCC CAAGAAGGTC 
AGAAGGTTCC CCCATCACCG GTTAGCCCAC CAGCCAGTGG ACCTGAGGGA AGGGGTGGCC 
AAGGCCTACA GTGTCGTGAA GGAGGGAATC GCAGACACGG CTCAGACCAT TTATGAAACT 
GCCGCTCGGG AACACGAGAG CCGGGGGGTG ACGGGCGCCG TGGGGGAAGT CCTGCGCCAG 
CTCCCCCCGG CCGTGGTGAA ACCTCTGATC GTCGCCACAG AAGCGACATC GAACGTCCTG 
GGCGGCATGA GAAACCAGAT CAGACCAGAT GTTCGGCAGG ACGAGTCGCA GAAGTGGCGC 
CATGGGGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_012099022.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954412.1 Ovis aries autophagy related 2B (ATG2B), transcript variant X3, mRNA.

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CloneID	OSm42092
Clone ID Related Accession (Same CDS sequence)	XM_012099022.1
Accession Version	XM_012099022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	autophagy-related protein 2 homolog B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTGGT CAAGCTTCAT GACCAGCAGT ATGCAACTGG CAAAAGAATG TCTTAGCCAG 
AAACTAACAG ATGAACAAGG AGAAGGATCT CAGCCTTTTG AAGGACTTGA AAAATTTGCT 
GAAACCATCG AAACAGTACT AAGAAGAGTA AAAGTCACTT TTATAGACAC TGTCTTGAGA 
ATTGAACATG TGCCAGAAAA TTCCAAAACT GGAACTGCAC TTGAAATTCG AATAGAAAGA 
ACCATGTACT GTGATGAAAC TGCTGACGAG TCCTCAGGAA TTAATGTACA TCAACCCACA 
GCTTTTGCTC ACAAGTTACT TCAGCTCTCT GGAGTGTCTC TCTTCTGGGA TGAGTTTTCT 
GCATCTGCAA AATCCTCCCC AGTGTGTTCA ACTGCGCCAG TGGAAACAGA GCCAAAGCTG 
TCACCTAGCT GGAATCCAAA AATTGTTTAT GAGCCTCACC CACAACTAAC AAGGAATTTG 
CCAGAAGTAG CACCTTCTGA CCCAGTGCAG ATAGGAGGGC TCAATGGTAG GCTGGAGTTG 
AGTCTCACAT TGAAACAGAA TGAAGTACTT CCTGGAGCTA AGTTGGATAT TGATGGACAG 
ATAGACTCTA TACATCTATT CCTGTCACCC AGGCAGGTGC ACTTGCTTTT GGACATGTTA 
GCAGCTATTG CTGGACCAGA AAATTCAAGC AAAATTGGAT TAGCTAATAA AGATAGAAAA 
AATCGACCTA TGCAGCAAGA AGATGAATAT CGAATTCAAA TGGAATTGAA CCGATATTAT 
TTGAGGAAAG ATTCCCTCTC TGCTGGCGTG TCCTCAGAGC AAAGCTTTTA TGAGACAGAA 
ACGGCTCGTA CACCTTCTAG CCGGGAAGAA GAAGTTTTCT TCTCCATGGC TGATATGGAC 
ATGTCGCACA GTCTCTCTTC TCTTCCACCC CTTGGGGAAC CCCCAAATAT GGACCTTGAG 
TTATCATTAA CTAGTACATA TACAAACACC CCAGCAGGAT CTCCATTAAG TACTACTGTG 
CTTCAGCCAA CATGGGGAGA CTTCCTTGAT CATCATAAAG AACAGCCAGT AAGAGGATCA 
GCGTTACCCT CCAACCTAGT TCACCCAGCA TCTTTACAAA AGACTGCTCT TCCCTCTAGG 
TCTGTTTCAG TGGATGAATC CAGGCCTGAA CTTATTTTTA GAGTAGCTGT GGGAACGTTT 
TCAGTCTCTG TGCTTCACAT TGATCCTTTA TCTCCACCTG AATCATCACT GAATGTTAAT 
CCATTGACAC CAATGGCGAT AGCTTTCTTT ACTTGTATAG AAAAAATTGA TCCAGCACAA 
TTTTCAACAG AAGATTTTAA GTCTTTTCGA GCAGTATTTG CAGAAGCTTG CTCACATGAT 
CACCTTAGAT TTATAGGCAC TGGCATCAAA GTGTCCTATG AACAAAGACA AAGATCAGCT 
TCCCGATATT TCAGTACTGA TATGTCCATT GGGCAAATGG AACTTTTGGA ATGCTTGTTT 
CCGACTGATT CTCATTCTGT TCCTCCTCAC TATACAGAGC TTTTGATGTT CCATTCCAAA 
GAACGAACTG ATTCTCACCT CCCTGTGTGT CTTCAGCTTC ATTATAAGCA TTCTGAGAAT 
AAAGGACCTC AGGGTAGTCA AGCAAGACTT AGTTCCGTTC CTCAGAAGGC AGAATTACAG 
GTTAAGTTAA ATCCAGTGTT TTGTGAGCTG GATATCAGTA TTGTGGACAG GTTAAATTCC 
TTGCTTCAAC CGCAGAAACT CACTACAGTG GAGATGATGG CATCACATAT GTATACTTCA 
TATAATAAGC ATATTAGTTT GCACAAAGCT TTCACTGAAG TATTTCTAGA TGACTCACAG 
AATCCTGCAA ACTGCCGGGT ATCAGTACAA GTTGCCACAC CAGCATTAAA CCTTTCTGTT 
CGCTTCCCAA TACCTGATCT TCGATCAGAT CAAGAGAGAG GACCATGGTT TAAGAAGTCA 
CTTCAGAAGG AGATTCTTCA TTTAGCATTC ACAGATCTAG AGTTTAAAAC TGAATTCCTA 
GGAGGATCAA CCCCTGAACA AGTTAAATTG GAGCTTACAT TTAGAGAACT AGTTGGGTCA 
TTCCAGGAAG ATAAAGGAGA ACCATCCATT AAGTTTTTCC ATGTGGCTAG TGGAGGAGAT 
GGAGATACAA CGTCATCAGA TGACTTTGAC TGGCCGCGAA TTGTACTGAA AGTGAACCCA 
CCAGCCATGC ATTCCATTTT GGAGAGAATA GCAGCTGAGG AGGAAGAGGA AAGTGATGGC 
CACTATCAGG AGGAAGAGGA GGGAGGAGCT CATTCCCTGA AGGATGTCTG TGATCTGAGA 
AGGCCAGCCC CGTCTCCCTT CTCTTCCCGT AGAGTAATGT TTGAAAATGA ACAGATGGTG 
ATGCCAGGAG ACCCTGTAGA AATGACCGAG TTTCAGAATA AAGCAATCAG CAATTCTCAC 
TATGTGCTGG AACTTACATT ACCCAATATT CATATAACAC TGCCTAATAA AAGCTTTTAT 
GAGAAGCTTT ATAATAGGAT CTTTAATGAT TTGCTGCTAT GGGAACCAAC AGCTCCTTCA 
CCAGTAGAGA CATTTGAAAA TATTTCGTAT GGTATTGGGC TTTCAGTAGC CAGTCAGCTG 
ATTAATACCT TCAGCAAGGA TAGTTTTAGT CCATTTAAAT CTACAGTTCA CTATGATGAG 
GAAAGTGGAT CTGAGGAGGA GACTTTGCAG TATTTTTCTA CTGTTGATCC CAATTATCGT 
TCTCGTAGGA AAAAAAAACT AGACTCCCAG AACAAGAACT CACAGAGTTT TCTCTCAGTT 
CTTCTGAGTA TTAATCATGG GTTAATTGCA GTGTTTACAG ATGTAAAGCA AGATAATGGA 
GATCTGTTGG AAAACAAGCA TGGTGAATTC TGGTTCGAGT TCAGCAGCGG CTCGTTGTTT 
TGTGTGACAA AGTATGAAGG CTTTGACGAC AAGCACTACG TGTGCCTTCA CTGCAGCAGC 
TTCAGTCTCT ACCACAAAGG CATAGTGGAT GGAGCAGTTC CTTCTACAGA AACACGACTG 
CCTAGTTCAA CCCGCCCACA TTGGTTAGAA CCTACTATTT ATTCCTCTGA AGAAGATGGC 
CTCAGTCGAG CTTCTTCAGA TGGTGTTGGA GGAGACACTT TGAATATGCT GTCTGTTGCA 
GTTAAAATAG TGTCTGATAA ATCAGAGTCC AATACAAAGG AGTTTCTCAT TGCTGTAGGG 
CTAAAAGGAG CTACTCTCCA GCATAGAATG CTTCCTTCTG GGCTTAGCTG GCATGAGCAG 
ATTTTATACT TCTTGAATAT TGCTGATGAA CCTGTTCTGG GATACAATCC TCCAACTTCA 
TTTACAACCT TTCATGTTCA TCTCTGGAGC TGTGCGCTTG ATTATAGGCC TCTTTATTTG 
CCAATCCGAT CCCTTCTTAC TGTTGAAACA TTCAGCGTTT CAAGTAGTGT TGCCTTGGAT 
AAATCATCTT CAACTCTCAG AATAATTTTG GATGAAGCTG CTTTACACCT GTCTGACAAG 
TGTAATACTG TTACTATAAA TTTGAATAGA GATTATGTTC GTGTGATGGA TATGGGACTT 
TTGGAATTAA CCATAACTGC AGTAAAGTCT GATTCCGATG GAGAGCAAAC TGAACCACAC 
TTTGAATTGC ACTGTTCGAG TGACGTTGTC CATGTTAGAA CGTGCTCAGA CTCTTGTGCT 
GCTCTAATGA ATCTAATTCA ATACATTGCA AGCTATGGGG ACTTACATGC CACTAACAAG 
GCAGACGTGA AGCCAGGAGC TCCTCAAAGA AAGGTGAAGG TAGATTCTGT TGGTCGGTCG 
TCCTCACGTG GTCCAGTACT TCCTGAAGCA GATCAACAGA TACTACGAGA TTTGATGAGT 
GATGCTATGG AGGAGATTGA CATGCAAGAA GCAGCTTCAT CTGTAAAACC ACAGTCTAAT 
GGTGTTTCCG ATGAAAAATC TCAAGTTCAG GAACCATGTT GTTCAGACCT CTTCCTGTTT 
CCTGATGAGA GTGGAAACGT GTCCCAGGAG CCTGGCCCCA CTTACGCCTC ATTCACCCAC 
CACTTGATTA GTGATGCAGT GACAGGCGTG CCCACGGAAA ATGACGACTT TTGCATTCTT 
TTTGCACCAA GAGCAACTGT TCAGGAGAAG GAAGAAGAAC CAGTTGTAAA AATAATGGTT 
GATGATGCAA TTGTGATAAA AGACAATTAT TTTAGTCTGC CTGTTACGAA GACAGATACT 
AGCAAAGCCC CGTTACACTT TCCCGTTCCT GTCGTTCGGT ACCTGGTTAA AGAAGTCTCT 
CTCGTCTGGC ATCTTTATGG AGGAAAAGAT TTTGGAACAG CCCCTCCTAC TTCTCCAGCT 
AAAAGTTACA TTAGTCCCCA TAGCTCACCT TCTCACACAC CTACAAGACA TGGACATAAT 
ATGGTATGTG GGGGAAAAGG AAGAAACCAT GACTTTTTAA TGGAAATACA GTTAAGCAAG 
GTGAAGTTTC AGCATGAGGT CTACCCGCCC TGCAAACCAG AATGCGAGTC TAGCCTTGTG 
GAGCACCCGG TGTCCCGGCA GGTGTTCATC GTGCAGGATC TTGAAATTCG AGATCGTCTG 
GCAACATCAC AAATGAATAA ATTTTTGTAC CTGTATTGCA GTAAAGAAAT GCCTCGGAAA 
GCTCATTCCA ACATGTTGAC AATTAAAGCA TTACATGTGC GTCCAGAGTC TGGCAGGTCC 
CCACAGGAGT GCTGCCTAAG AGTATCACTA ATGCCACTTC GCCTCAATAT TGACCAGGAT 
GCCTTGTTCT TCTTGAAGGA TTTCTTCACA AGTCTTTCTG CAGAAGTAGA GCTGCTCATG 
ACTCCAGATC CAGAAGTTAA AAAGTCTCCT GGGGCTGATG TCACCTGCAG TTTGCCAAGG 
CATTTAAGCA CCTCAAAAGA GCCAAATCTA GTTCTTTCTT TCCCTGGGCC AAAGCAGCCT 
TCCCCAAATG GCAGTGCCAA TTCAGTGCAA GCGGTTAATG GGGAAGAAGA GAAGGACTTC 
TCTCCTGAAG AAGCGTTGTT TAGTGATCAG CCCGTGTTTT TTAGAGAATT TAGATTCACA 
TCAGAAGTTC CTATTCGACT TGATTATCAT GGGAAACATG TATCAATGGA TCAGGGTACA 
TTAGCTGGGA TTTTGATTGG TCTCGCCCAG TTAAACTGCT CTGAACTAAA GCTGAAGAGG 
CTTTTCTATC GGCATGGTTT GCTAGGTGTG GACAAATTAT TCTCATATGC AATCACCGAA 
TGGCTTACTG ACATTAAGAA GAACCAGCTT CCAGGAATCC TGGGAGGTGT TGGGCCTATG 
CATTCACTAG TACAACTAGT GCAAGGCCTC AAGGACTTGG TCTGGTTACC AATTGAGCAG 
TACCGAAAGG ACGGTCGCAT TGTCAGAGGA TTTCAGAGAG GGGCTGCCTC CTTTGGCACC 
TCGACGGCAA TGGCTGCTCT CGAGCTCACA AACAGAATGG TTCAAACCAT ACAGGCAGCG 
GCAGAGACTG CGTATGACAT GGTGTCTCCA GGCACCCTTT CTATCGAGCC CAAGAAGGTC 
AGAAGGTTCC CCCATCACCG GTTAGCCCAC CAGCCAGTGG ACCTGAGGGA AGGGGTGGCC 
AAGGCCTACA GTGTCGTGAA GGAGGGAATC GCAGACACGG CTCAGACCAT TTATGAAACT 
GCCGCTCGGG AACACGAGAG CCGGGGGGTG ACGGGCGCCG TGGGGGAAGT CCTGCGCCAG 
CTCCCCCCGG CCGTGGTGAA ACCTCTGATC GTCGCCACAG AAGCGACATC GAACGTCCTG 
GGCGGCATGA GAAACCAGAT CAGACCAGAT GTTCGGCAGG ACGAGTCCCA GAAGTGGCGC 
CATGGGGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954412.1
CDS	353..6247
Translation	MYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSVSVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQGSQARLSSVPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSQNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLIAVFTDVKQDNGDLLENKHGEFWFEFSSGSLFCVTKYEGFDDKHYVCLHCSSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVKVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDAIVIKDNYFSLPVTKTDTSKAPLHFPVPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEVELLMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_012099022.1
Organism	Ovis aries(sheep)
Definition	Ovis aries autophagy related 2B (ATG2B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012099022.1

ORF Insert Sequence:

ATGTACTGGT CAAGCTTCAT GACCAGCAGT ATGCAACTGG CAAAAGAATG TCTTAGCCAG 
AAACTAACAG ATGAACAAGG AGAAGGATCT CAGCCTTTTG AAGGACTTGA AAAATTTGCT 
GAAACCATCG AAACAGTACT AAGAAGAGTA AAAGTCACTT TTATAGACAC TGTCTTGAGA 
ATTGAACATG TGCCAGAAAA TTCCAAAACT GGAACTGCAC TTGAAATTCG AATAGAAAGA 
ACCATGTACT GTGATGAAAC TGCTGACGAG TCCTCAGGAA TTAATGTACA TCAACCCACA 
GCTTTTGCTC ACAAGTTACT TCAGCTCTCT GGAGTGTCTC TCTTCTGGGA TGAGTTTTCT 
GCATCTGCAA AATCCTCCCC AGTGTGTTCA ACTGCGCCAG TGGAAACAGA GCCAAAGCTG 
TCACCTAGCT GGAATCCAAA AATTGTTTAT GAGCCTCACC CACAACTAAC AAGGAATTTG 
CCAGAAGTAG CACCTTCTGA CCCAGTGCAG ATAGGAGGGC TCAATGGTAG GCTGGAGTTG 
AGTCTCACAT TGAAACAGAA TGAAGTACTT CCTGGAGCTA AGTTGGATAT TGATGGACAG 
ATAGACTCTA TACATCTATT CCTGTCACCC AGGCAGGTGC ACTTGCTTTT GGACATGTTA 
GCAGCTATTG CTGGACCAGA AAATTCAAGC AAAATTGGAT TAGCTAATAA AGATAGAAAA 
AATCGACCTA TGCAGCAAGA AGATGAATAT CGAATTCAAA TGGAATTGAA CCGATATTAT 
TTGAGGAAAG ATTCCCTCTC TGCTGGCGTG TCCTCAGAGC AAAGCTTTTA TGAGACAGAA 
ACGGCTCGTA CACCTTCTAG CCGGGAAGAA GAAGTTTTCT TCTCCATGGC TGATATGGAC 
ATGTCGCACA GTCTCTCTTC TCTTCCACCC CTTGGGGAAC CCCCAAATAT GGACCTTGAG 
TTATCATTAA CTAGTACATA TACAAACACC CCAGCAGGAT CTCCATTAAG TACTACTGTG 
CTTCAGCCAA CATGGGGAGA CTTCCTTGAT CATCATAAAG AACAGCCAGT AAGAGGATCA 
GCGTTACCCT CCAACCTAGT TCACCCAGCA TCTTTACAAA AGACTGCTCT TCCCTCTAGG 
TCTGTTTCAG TGGATGAATC CAGGCCTGAA CTTATTTTTA GAGTAGCTGT GGGAACGTTT 
TCAGTCTCTG TGCTTCACAT TGATCCTTTA TCTCCACCTG AATCATCACT GAATGTTAAT 
CCATTGACAC CAATGGCGAT AGCTTTCTTT ACTTGTATAG AAAAAATTGA TCCAGCACAA 
TTTTCAACAG AAGATTTTAA GTCTTTTCGA GCAGTATTTG CAGAAGCTTG CTCACATGAT 
CACCTTAGAT TTATAGGCAC TGGCATCAAA GTGTCCTATG AACAAAGACA AAGATCAGCT 
TCCCGATATT TCAGTACTGA TATGTCCATT GGGCAAATGG AACTTTTGGA ATGCTTGTTT 
CCGACTGATT CTCATTCTGT TCCTCCTCAC TATACAGAGC TTTTGATGTT CCATTCCAAA 
GAACGAACTG ATTCTCACCT CCCTGTGTGT CTTCAGCTTC ATTATAAGCA TTCTGAGAAT 
AAAGGACCTC AGGGTAGTCA AGCAAGACTT AGTTCCGTTC CTCAGAAGGC AGAATTACAG 
GTTAAGTTAA ATCCAGTGTT TTGTGAGCTG GATATCAGTA TTGTGGACAG GTTAAATTCC 
TTGCTTCAAC CGCAGAAACT CACTACAGTG GAGATGATGG CATCACATAT GTATACTTCA 
TATAATAAGC ATATTAGTTT GCACAAAGCT TTCACTGAAG TATTTCTAGA TGACTCACAG 
AATCCTGCAA ACTGCCGGGT ATCAGTACAA GTTGCCACAC CAGCATTAAA CCTTTCTGTT 
CGCTTCCCAA TACCTGATCT TCGATCAGAT CAAGAGAGAG GACCATGGTT TAAGAAGTCA 
CTTCAGAAGG AGATTCTTCA TTTAGCATTC ACAGATCTAG AGTTTAAAAC TGAATTCCTA 
GGAGGATCAA CCCCTGAACA AGTTAAATTG GAGCTTACAT TTAGAGAACT AGTTGGGTCA 
TTCCAGGAAG ATAAAGGAGA ACCATCCATT AAGTTTTTCC ATGTGGCTAG TGGAGGAGAT 
GGAGATACAA CGTCATCAGA TGACTTTGAC TGGCCGCGAA TTGTACTGAA AGTGAACCCA 
CCAGCCATGC ATTCCATTTT GGAGAGAATA GCAGCTGAGG AGGAAGAGGA AAGTGATGGC 
CACTATCAGG AGGAAGAGGA GGGAGGAGCT CATTCCCTGA AGGATGTCTG TGATCTGAGA 
AGGCCAGCCC CGTCTCCCTT CTCTTCCCGT AGAGTAATGT TTGAAAATGA ACAGATGGTG 
ATGCCAGGAG ACCCTGTAGA AATGACCGAG TTTCAGAATA AAGCAATCAG CAATTCTCAC 
TATGTGCTGG AACTTACATT ACCCAATATT CATATAACAC TGCCTAATAA AAGCTTTTAT 
GAGAAGCTTT ATAATAGGAT CTTTAATGAT TTGCTGCTAT GGGAACCAAC AGCTCCTTCA 
CCAGTAGAGA CATTTGAAAA TATTTCGTAT GGTATTGGGC TTTCAGTAGC CAGTCAGCTG 
ATTAATACCT TCAGCAAGGA TAGTTTTAGT CCATTTAAAT CTACAGTTCA CTATGATGAG 
GAAAGTGGAT CTGAGGAGGA GACTTTGCAG TATTTTTCTA CTGTTGATCC CAATTATCGT 
TCTCGTAGGA AAAAAAAACT AGACTCCCAG AACAAGAACT CACAGAGTTT TCTCTCAGTT 
CTTCTGAGTA TTAATCATGG GTTAATTGCA GTGTTTACAG ATGTAAAGCA AGATAATGGA 
GATCTGTTGG AAAACAAGCA TGGTGAATTC TGGTTCGAGT TCAGCAGCGG CTCGTTGTTT 
TGTGTGACAA AGTATGAAGG CTTTGACGAC AAGCACTACG TGTGCCTTCA CTGCAGCAGC 
TTCAGTCTCT ACCACAAAGG CATAGTGGAT GGAGCAGTTC CTTCTACAGA AACACGACTG 
CCTAGTTCAA CCCGCCCACA TTGGTTAGAA CCTACTATTT ATTCCTCTGA AGAAGATGGC 
CTCAGTCGAG CTTCTTCAGA TGGTGTTGGA GGAGACACTT TGAATATGCT GTCTGTTGCA 
GTTAAAATAG TGTCTGATAA ATCAGAGTCC AATACAAAGG AGTTTCTCAT TGCTGTAGGG 
CTAAAAGGAG CTACTCTCCA GCATAGAATG CTTCCTTCTG GGCTTAGCTG GCATGAGCAG 
ATTTTATACT TCTTGAATAT TGCTGATGAA CCTGTTCTGG GATACAATCC TCCAACTTCA 
TTTACAACCT TTCATGTTCA TCTCTGGAGC TGTGCGCTTG ATTATAGGCC TCTTTATTTG 
CCAATCCGAT CCCTTCTTAC TGTTGAAACA TTCAGCGTTT CAAGTAGTGT TGCCTTGGAT 
AAATCATCTT CAACTCTCAG AATAATTTTG GATGAAGCTG CTTTACACCT GTCTGACAAG 
TGTAATACTG TTACTATAAA TTTGAATAGA GATTATGTTC GTGTGATGGA TATGGGACTT 
TTGGAATTAA CCATAACTGC AGTAAAGTCT GATTCCGATG GAGAGCAAAC TGAACCACAC 
TTTGAATTGC ACTGTTCGAG TGACGTTGTC CATGTTAGAA CGTGCTCAGA CTCTTGTGCT 
GCTCTAATGA ATCTAATTCA ATACATTGCA AGCTATGGGG ACTTACATGC CACTAACAAG 
GCAGACGTGA AGCCAGGAGC TCCTCAAAGA AAGGTGAAGG TAGATTCTGT TGGTCGGTCG 
TCCTCACGTG GTCCAGTACT TCCTGAAGCA GATCAACAGA TACTACGAGA TTTGATGAGT 
GATGCTATGG AGGAGATTGA CATGCAAGAA GCAGCTTCAT CTGTAAAACC ACAGTCTAAT 
GGTGTTTCCG ATGAAAAATC TCAAGTTCAG GAACCATGTT GTTCAGACCT CTTCCTGTTT 
CCTGATGAGA GTGGAAACGT GTCCCAGGAG CCTGGCCCCA CTTACGCCTC ATTCACCCAC 
CACTTGATTA GTGATGCAGT GACAGGCGTG CCCACGGAAA ATGACGACTT TTGCATTCTT 
TTTGCACCAA GAGCAACTGT TCAGGAGAAG GAAGAAGAAC CAGTTGTAAA AATAATGGTT 
GATGATGCAA TTGTGATAAA AGACAATTAT TTTAGTCTGC CTGTTACGAA GACAGATACT 
AGCAAAGCCC CGTTACACTT TCCCGTTCCT GTCGTTCGGT ACCTGGTTAA AGAAGTCTCT 
CTCGTCTGGC ATCTTTATGG AGGAAAAGAT TTTGGAACAG CCCCTCCTAC TTCTCCAGCT 
AAAAGTTACA TTAGTCCCCA TAGCTCACCT TCTCACACAC CTACAAGACA TGGACATAAT 
ATGGTATGTG GGGGAAAAGG AAGAAACCAT GACTTTTTAA TGGAAATACA GTTAAGCAAG 
GTGAAGTTTC AGCATGAGGT CTACCCGCCC TGCAAACCAG AATGCGAGTC TAGCCTTGTG 
GAGCACCCGG TGTCCCGGCA GGTGTTCATC GTGCAGGATC TTGAAATTCG AGATCGTCTG 
GCAACATCAC AAATGAATAA ATTTTTGTAC CTGTATTGCA GTAAAGAAAT GCCTCGGAAA 
GCTCATTCCA ACATGTTGAC AATTAAAGCA TTACATGTGC GTCCAGAGTC TGGCAGGTCC 
CCACAGGAGT GCTGCCTAAG AGTATCACTA ATGCCACTTC GCCTCAATAT TGACCAGGAT 
GCCTTGTTCT TCTTGAAGGA TTTCTTCACA AGTCTTTCTG CAGAAGTAGA GCTGCTCATG 
ACTCCAGATC CAGAAGTTAA AAAGTCTCCT GGGGCTGATG TCACCTGCAG TTTGCCAAGG 
CATTTAAGCA CCTCAAAAGA GCCAAATCTA GTTCTTTCTT TCCCTGGGCC AAAGCAGCCT 
TCCCCAAATG GCAGTGCCAA TTCAGTGCAA GCGGTTAATG GGGAAGAAGA GAAGGACTTC 
TCTCCTGAAG AAGCGTTGTT TAGTGATCAG CCCGTGTTTT TTAGAGAATT TAGATTCACA 
TCAGAAGTTC CTATTCGACT TGATTATCAT GGGAAACATG TATCAATGGA TCAGGGTACA 
TTAGCTGGGA TTTTGATTGG TCTCGCCCAG TTAAACTGCT CTGAACTAAA GCTGAAGAGG 
CTTTTCTATC GGCATGGTTT GCTAGGTGTG GACAAATTAT TCTCATATGC AATCACCGAA 
TGGCTTACTG ACATTAAGAA GAACCAGCTT CCAGGAATCC TGGGAGGTGT TGGGCCTATG 
CATTCACTAG TACAACTAGT GCAAGGCCTC AAGGACTTGG TCTGGTTACC AATTGAGCAG 
TACCGAAAGG ACGGTCGCAT TGTCAGAGGA TTTCAGAGAG GGGCTGCCTC CTTTGGCACC 
TCGACGGCAA TGGCTGCTCT CGAGCTCACA AACAGAATGG TTCAAACCAT ACAGGCAGCG 
GCAGAGACTG CGTATGACAT GGTGTCTCCA GGCACCCTTT CTATCGAGCC CAAGAAGGTC 
AGAAGGTTCC CCCATCACCG GTTAGCCCAC CAGCCAGTGG ACCTGAGGGA AGGGGTGGCC 
AAGGCCTACA GTGTCGTGAA GGAGGGAATC GCAGACACGG CTCAGACCAT TTATGAAACT 
GCCGCTCGGG AACACGAGAG CCGGGGGGTG ACGGGCGCCG TGGGGGAAGT CCTGCGCCAG 
CTCCCCCCGG CCGTGGTGAA ACCTCTGATC GTCGCCACAG AAGCGACATC GAACGTCCTG 
GGCGGCATGA GAAACCAGAT CAGACCAGAT GTTCGGCAGG ACGAGTCCCA GAAGTGGCGC 
CATGGGGATG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_012112891.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011968281.1 Ovis aries musimon autophagy related 2B (ATG2B), transcript variant X1, mRNA.

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CloneID	OOv02811
Clone ID Related Accession (Same CDS sequence)	XM_012112891.1
Accession Version	XM_012112891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	autophagy-related protein 2 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942251.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CTTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACG GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGAGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCGTCT GGTTCTGAGC CCATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAACT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAATTTG CTGAAACCAT CGAAACAGTA 
CTGAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTCTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAATGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGCGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAGCCTCA CCCACAACTA ACAAGGAATT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATACATCTA 
TTCCTGTCAC CCAGGCAGGT GCACTTGCTT TTGGACATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAATCGACC TATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGATATT ATTTGAGGAA AGATTCCCTC 
TCTGCCGGCG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGAT CAGCGTTACC CTCCAACCTA 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTTCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAGTCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAAATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTTC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCTTGT TTCCGACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGGTAGT 
CAAGCAAGAC TTAGTTCCGT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCGCAGAAA 
CTCACTACAG TGGAGATGAT GGCATCACAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGACTCAC AGAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAGCTTAC ATTTAGAGAA CTAGTTGGGT CATTCCAGGA AGATAAAGGA 
GAACCATCCA TTAAGTTTTT CCATGTGGCT AGTGGCGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTG AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGATG GCCACTATCA GGAGGAAGAG 
GAGGGAGGAG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC CCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACCG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ATTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTGCCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ATTTGCTGCT ATGGGAACCA ACAGCTCCTT CACCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCATTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TATTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTCGA GTTCAGCAGC GGCTCGTTGT TTTGTGTGAC AAAGTATGAA 
GGCTTTGACG ACAAGCACTA CGTGTGCCTT CACTGCAGCA GCTTCAGTCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATACAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCGCT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCCCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTCGCCTTGG ATAAATCATC TTCAACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCAC ACTTTGAATT GCACTGTTCG 
AGTGACGTTG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATACATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTGAA GGTAGATTCT GTTGGTCGGT CATCCTCACG TGGTCCAGTA 
CTTCCTGAAG CAGATCAACA GATACTACGA GATTTGATGA GTGATGCTAT GGAGGAGATT 
GACATGCAAG AAGCAGCTTC ATCTGTAAAA CCACAGTCTA ATGGTGTTTC CGATGAAAAA 
TCTCAAGTTC AGGAACCATG TTGTTCAGAC CTCTTCCTGT TTCCTGATGA GAGTGGAAAC 
GTGTCCCAGG AGCCTGGCCC CACTTACGCC TCATTCACCC ACCACTTGAT TAGTGATGCA 
GTGACAGGCG TGCCCACGGA AAATGACGAC TTTTGCATTC TTTTTGCACC AAGAGCAACT 
GTTCAGGAGA AGGAAGAAGA ACCAGTTGTA AAAATAATGG TTGATGATGC AATTGTGATA 
AAAGAGAATT ATTTTAGTCT GCCTGTTACG AAGACAGACA CTAGCAAAGC CCCGTTACAC 
TTTCCCGTTC CTGTCGTTCG GTACCTGGTT AAAGAAGTCT CTCTCGTCTG GCATCTTTAT 
GGAGGAAAAG ATTTTGGAAC AGCCCCTCCT ACTTCTCCAG CTAAAAGTTA CATTAGTCCC 
CATAGCTCAC CTTCTCACAC ACCTACAAGA CATGGACATA ATATGGTATG TGGGGGAAAA 
GGAAGAAACC ATGACTTTTT AATGGAAATA CAGTTAAGCA AGGTAAAGTT TCAGCATGAG 
GTCTACCCGC CCTGCAAACC AGAATGCGAG TCTAGCCTTG TGGAGCACCC GGTGTCCCGG 
CAGGTGTTCA TCGTGCAGGA TCTTGAAATT CGAGATCGTC TGGCAACATC ACAAATGAAT 
AAATTTTTGT ACCTGTATTG CAGTAAAGAA ATGCCTCGGA AAGCTCATTC CAACATGTTG 
ACAATTAAAG CATTACACGT GCGTCCAGAG TCTGGCAGGT CCCCACAGGA GTGCTGCCTA 
AGAGTATCAC TAATGCCACT TCGCCTCAAT ATTGACCAGG ATGCCTTGTT CTTCTTGAAG 
GATTTCTTCA CAAGTCTTTC TGCAGAAGTA GAGCTGCTCA TGACTCCAGA TCCAGAAGTT 
AAAAAGTCTC CTGGGGCTGA TGTCACCTGC AGTTTGCCAA GGCATTTAAG CACCTCAAAA 
GAGCCAAATC TAGTTCTTTC TTTCCCTGGG CCAAAGCAGC CTTCCCCAAA TGGCAGTGCC 
AATTCAGTGC AAGCGGTTAA TGGGGAAGAA GAGAAGGACT TCTCTCCTGA AGAAGCGTTG 
TTTAGTGATC AGCCCGTGTT TTTTAGAGAA TTTAGATTCA CATCAGAAGT TCCTATTCGA 
CTTGATTATC ATGGGAAACA TGTATCAATG GATCAGGGTA CATTAGCTGG GATTTTGATT 
GGTCTCGCCC AGTTAAACTG CTCTGAACTA AAGCTGAAGA GGCTTTTCTA TCGGCATGGT 
TTGCTAGGTG TGGACAAATT ATTCTCATAT GCAATCACCG AATGGCTTAC TGACATTAAG 
AAGAACCAGC TTCCAGGAAT CCTGGGAGGT GTTGGGCCTA TGCATTCACT AGTACAACTA 
GTGCAAGGCC TCAAGGACTT GGTCTGGTTA CCAATTGAGC AGTACCGAAA GGACGGTCGC 
ATTGTCAGAG GATTTCAGAG AGGGGCTGCC TCCTTTGGCA CCTCGACGGC GATGGCTGCT 
CTCGAGCTCA CAAACAGAAT GGTTCAAACC ATACAGGCAG CGGCAGAGAC TGCGTATGAC 
ATGGTGTCTC CAGGCACCCT TTCTATCGAG CCCAAGAAGG TCAGAAGGTT CCCCCATCAC 
CGGTTAGCCC ACCAGCCAGT GGACCTGAGG GAAGGGGTGG CCAAGGCCTA CAGTGTCGTG 
AAGGAGGGAA TCGCAGACAC GGCTCAGACC ATTTATGAAA CTGCCGCTCG GGAACACGAG 
AGCCGGGGGG TGACGGGCGC CGTGGGGGAA GTCCTGCGCC AGCTCCCCCC GGCCGTGGTG 
AAACCTCTGA TCGTCGCCAC AGAAGCGACA TCGAACGTCC TGGGCGGCAT GAGAAACCAG 
ATCAGACCAG ATGTTCGGCA GGACGAGTCG CAGAAGTGGC GCCATGGGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011968281.1
CDS	2..6238
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRLASGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSVSVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQGSQARLSSVPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSQNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLIAVFTDVKQDNGDLLENKHGEFWFEFSSGSLFCVTKYEGFDDKHYVCLHCSSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVKVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDAIVIKENYFSLPVTKTDTSKAPLHFPVPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEVELLMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_012112891.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon autophagy related 2B (ATG2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012112891.1

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CTTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACG GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGAGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCGTCT GGTTCTGAGC CCATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAACT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAATTTG CTGAAACCAT CGAAACAGTA 
CTGAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTCTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAATGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGCGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAGCCTCA CCCACAACTA ACAAGGAATT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATACATCTA 
TTCCTGTCAC CCAGGCAGGT GCACTTGCTT TTGGACATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAATCGACC TATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGATATT ATTTGAGGAA AGATTCCCTC 
TCTGCCGGCG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGAT CAGCGTTACC CTCCAACCTA 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTTCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAGTCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAAATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTTC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCTTGT TTCCGACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGGTAGT 
CAAGCAAGAC TTAGTTCCGT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCGCAGAAA 
CTCACTACAG TGGAGATGAT GGCATCACAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGACTCAC AGAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAGCTTAC ATTTAGAGAA CTAGTTGGGT CATTCCAGGA AGATAAAGGA 
GAACCATCCA TTAAGTTTTT CCATGTGGCT AGTGGCGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTG AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGATG GCCACTATCA GGAGGAAGAG 
GAGGGAGGAG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC CCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACCG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ATTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTGCCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ATTTGCTGCT ATGGGAACCA ACAGCTCCTT CACCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCATTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TATTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTCGA GTTCAGCAGC GGCTCGTTGT TTTGTGTGAC AAAGTATGAA 
GGCTTTGACG ACAAGCACTA CGTGTGCCTT CACTGCAGCA GCTTCAGTCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATACAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCGCT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCCCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTCGCCTTGG ATAAATCATC TTCAACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCAC ACTTTGAATT GCACTGTTCG 
AGTGACGTTG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATACATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTGAA GGTAGATTCT GTTGGTCGGT CATCCTCACG TGGTCCAGTA 
CTTCCTGAAG CAGATCAACA GATACTACGA GATTTGATGA GTGATGCTAT GGAGGAGATT 
GACATGCAAG AAGCAGCTTC ATCTGTAAAA CCACAGTCTA ATGGTGTTTC CGATGAAAAA 
TCTCAAGTTC AGGAACCATG TTGTTCAGAC CTCTTCCTGT TTCCTGATGA GAGTGGAAAC 
GTGTCCCAGG AGCCTGGCCC CACTTACGCC TCATTCACCC ACCACTTGAT TAGTGATGCA 
GTGACAGGCG TGCCCACGGA AAATGACGAC TTTTGCATTC TTTTTGCACC AAGAGCAACT 
GTTCAGGAGA AGGAAGAAGA ACCAGTTGTA AAAATAATGG TTGATGATGC AATTGTGATA 
AAAGAGAATT ATTTTAGTCT GCCTGTTACG AAGACAGACA CTAGCAAAGC CCCGTTACAC 
TTTCCCGTTC CTGTCGTTCG GTACCTGGTT AAAGAAGTCT CTCTCGTCTG GCATCTTTAT 
GGAGGAAAAG ATTTTGGAAC AGCCCCTCCT ACTTCTCCAG CTAAAAGTTA CATTAGTCCC 
CATAGCTCAC CTTCTCACAC ACCTACAAGA CATGGACATA ATATGGTATG TGGGGGAAAA 
GGAAGAAACC ATGACTTTTT AATGGAAATA CAGTTAAGCA AGGTAAAGTT TCAGCATGAG 
GTCTACCCGC CCTGCAAACC AGAATGCGAG TCTAGCCTTG TGGAGCACCC GGTGTCCCGG 
CAGGTGTTCA TCGTGCAGGA TCTTGAAATT CGAGATCGTC TGGCAACATC ACAAATGAAT 
AAATTTTTGT ACCTGTATTG CAGTAAAGAA ATGCCTCGGA AAGCTCATTC CAACATGTTG 
ACAATTAAAG CATTACACGT GCGTCCAGAG TCTGGCAGGT CCCCACAGGA GTGCTGCCTA 
AGAGTATCAC TAATGCCACT TCGCCTCAAT ATTGACCAGG ATGCCTTGTT CTTCTTGAAG 
GATTTCTTCA CAAGTCTTTC TGCAGAAGTA GAGCTGCTCA TGACTCCAGA TCCAGAAGTT 
AAAAAGTCTC CTGGGGCTGA TGTCACCTGC AGTTTGCCAA GGCATTTAAG CACCTCAAAA 
GAGCCAAATC TAGTTCTTTC TTTCCCTGGG CCAAAGCAGC CTTCCCCAAA TGGCAGTGCC 
AATTCAGTGC AAGCGGTTAA TGGGGAAGAA GAGAAGGACT TCTCTCCTGA AGAAGCGTTG 
TTTAGTGATC AGCCCGTGTT TTTTAGAGAA TTTAGATTCA CATCAGAAGT TCCTATTCGA 
CTTGATTATC ATGGGAAACA TGTATCAATG GATCAGGGTA CATTAGCTGG GATTTTGATT 
GGTCTCGCCC AGTTAAACTG CTCTGAACTA AAGCTGAAGA GGCTTTTCTA TCGGCATGGT 
TTGCTAGGTG TGGACAAATT ATTCTCATAT GCAATCACCG AATGGCTTAC TGACATTAAG 
AAGAACCAGC TTCCAGGAAT CCTGGGAGGT GTTGGGCCTA TGCATTCACT AGTACAACTA 
GTGCAAGGCC TCAAGGACTT GGTCTGGTTA CCAATTGAGC AGTACCGAAA GGACGGTCGC 
ATTGTCAGAG GATTTCAGAG AGGGGCTGCC TCCTTTGGCA CCTCGACGGC GATGGCTGCT 
CTCGAGCTCA CAAACAGAAT GGTTCAAACC ATACAGGCAG CGGCAGAGAC TGCGTATGAC 
ATGGTGTCTC CAGGCACCCT TTCTATCGAG CCCAAGAAGG TCAGAAGGTT CCCCCATCAC 
CGGTTAGCCC ACCAGCCAGT GGACCTGAGG GAAGGGGTGG CCAAGGCCTA CAGTGTCGTG 
AAGGAGGGAA TCGCAGACAC GGCTCAGACC ATTTATGAAA CTGCCGCTCG GGAACACGAG 
AGCCGGGGGG TGACGGGCGC CGTGGGGGAA GTCCTGCGCC AGCTCCCCCC GGCCGTGGTG 
AAACCTCTGA TCGTCGCCAC AGAAGCGACA TCGAACGTCC TGGGCGGCAT GAGAAACCAG 
ATCAGACCAG ATGTTCGGCA GGACGAGTCG CAGAAGTGGC GCCATGGGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_012112892.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011968282.1 Ovis aries musimon autophagy related 2B (ATG2B), transcript variant X2, mRNA.

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CloneID	OOv02812
Clone ID Related Accession (Same CDS sequence)	XM_012112892.1
Accession Version	XM_012112892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	autophagy-related protein 2 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942251.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CTTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACG GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGAGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCGTCT GGTTCTGAGC CCATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAACT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAATTTG CTGAAACCAT CGAAACAGTA 
CTGAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTCTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAATGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGCGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAGCCTCA CCCACAACTA ACAAGGAATT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATACATCTA 
TTCCTGTCAC CCAGGCAGGT GCACTTGCTT TTGGACATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAATCGACC TATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGATATT ATTTGAGGAA AGATTCCCTC 
TCTGCCGGCG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGAT CAGCGTTACC CTCCAACCTA 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTTCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAGTCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAAATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTTC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCTTGT TTCCGACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGGTAGT 
CAAGCAAGAC TTAGTTCCGT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCGCAGAAA 
CTCACTACAG TGGAGATGAT GGCATCACAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGACTCAC AGAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAGCTTAC ATTTAGAGAA CTAGTTGGGT CATTCCAGGA AGATAAAGGA 
GAACCATCCA TTAAGTTTTT CCATGTGGCT AGTGGCGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTG AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGATG GCCACTATCA GGAGGAAGAG 
GAGGGAGGAG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC CCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACCG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ATTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTGCCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ATTTGCTGCT ATGGGAACCA ACAGCTCCTT CACCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCATTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TATTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTCGA GTTCAGCAGC GGCTCGTTGT TTTGTGTGAC AAAGTATGAA 
GGCTTTGACG ACAAGCACTA CGTGTGCCTT CACTGCAGCA GCTTCAGTCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATACAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCGCT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCCCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTCGCCTTGG ATAAATCATC TTCAACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCAC ACTTTGAATT GCACTGTTCG 
AGTGACGTTG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATACATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTAGA TTCTGTTGGT CGGTCATCCT CACGTGGTCC AGTACTTCCT 
GAAGCAGATC AACAGATACT ACGAGATTTG ATGAGTGATG CTATGGAGGA GATTGACATG 
CAAGAAGCAG CTTCATCTGT AAAACCACAG TCTAATGGTG TTTCCGATGA AAAATCTCAA 
GTTCAGGAAC CATGTTGTTC AGACCTCTTC CTGTTTCCTG ATGAGAGTGG AAACGTGTCC 
CAGGAGCCTG GCCCCACTTA CGCCTCATTC ACCCACCACT TGATTAGTGA TGCAGTGACA 
GGCGTGCCCA CGGAAAATGA CGACTTTTGC ATTCTTTTTG CACCAAGAGC AACTGTTCAG 
GAGAAGGAAG AAGAACCAGT TGTAAAAATA ATGGTTGATG ATGCAATTGT GATAAAAGAG 
AATTATTTTA GTCTGCCTGT TACGAAGACA GACACTAGCA AAGCCCCGTT ACACTTTCCC 
GTTCCTGTCG TTCGGTACCT GGTTAAAGAA GTCTCTCTCG TCTGGCATCT TTATGGAGGA 
AAAGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATTAG TCCCCATAGC 
TCACCTTCTC ACACACCTAC AAGACATGGA CATAATATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAGTTA AGCAAGGTAA AGTTTCAGCA TGAGGTCTAC 
CCGCCCTGCA AACCAGAATG CGAGTCTAGC CTTGTGGAGC ACCCGGTGTC CCGGCAGGTG 
TTCATCGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAATTT 
TTGTACCTGT ATTGCAGTAA AGAAATGCCT CGGAAAGCTC ATTCCAACAT GTTGACAATT 
AAAGCATTAC ACGTGCGTCC AGAGTCTGGC AGGTCCCCAC AGGAGTGCTG CCTAAGAGTA 
TCACTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCTT GAAGGATTTC 
TTCACAAGTC TTTCTGCAGA AGTAGAGCTG CTCATGACTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGGG CTGATGTCAC CTGCAGTTTG CCAAGGCATT TAAGCACCTC AAAAGAGCCA 
AATCTAGTTC TTTCTTTCCC TGGGCCAAAG CAGCCTTCCC CAAATGGCAG TGCCAATTCA 
GTGCAAGCGG TTAATGGGGA AGAAGAGAAG GACTTCTCTC CTGAAGAAGC GTTGTTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTTCCTAT TCGACTTGAT 
TATCATGGGA AACATGTATC AATGGATCAG GGTACATTAG CTGGGATTTT GATTGGTCTC 
GCCCAGTTAA ACTGCTCTGA ACTAAAGCTG AAGAGGCTTT TCTATCGGCA TGGTTTGCTA 
GGTGTGGACA AATTATTCTC ATATGCAATC ACCGAATGGC TTACTGACAT TAAGAAGAAC 
CAGCTTCCAG GAATCCTGGG AGGTGTTGGG CCTATGCATT CACTAGTACA ACTAGTGCAA 
GGCCTCAAGG ACTTGGTCTG GTTACCAATT GAGCAGTACC GAAAGGACGG TCGCATTGTC 
AGAGGATTTC AGAGAGGGGC TGCCTCCTTT GGCACCTCGA CGGCGATGGC TGCTCTCGAG 
CTCACAAACA GAATGGTTCA AACCATACAG GCAGCGGCAG AGACTGCGTA TGACATGGTG 
TCTCCAGGCA CCCTTTCTAT CGAGCCCAAG AAGGTCAGAA GGTTCCCCCA TCACCGGTTA 
GCCCACCAGC CAGTGGACCT GAGGGAAGGG GTGGCCAAGG CCTACAGTGT CGTGAAGGAG 
GGAATCGCAG ACACGGCTCA GACCATTTAT GAAACTGCCG CTCGGGAACA CGAGAGCCGG 
GGGGTGACGG GCGCCGTGGG GGAAGTCCTG CGCCAGCTCC CCCCGGCCGT GGTGAAACCT 
CTGATCGTCG CCACAGAAGC GACATCGAAC GTCCTGGGCG GCATGAGAAA CCAGATCAGA 
CCAGATGTTC GGCAGGACGA GTCGCAGAAG TGGCGCCATG GGGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011968282.1
CDS	2..6232
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRLASGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSVSVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQGSQARLSSVPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSQNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLIAVFTDVKQDNGDLLENKHGEFWFEFSSGSLFCVTKYEGFDDKHYVCLHCSSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDAIVIKENYFSLPVTKTDTSKAPLHFPVPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEVELLMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_012112892.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon autophagy related 2B (ATG2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012112892.1

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CTTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACG GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGAGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCGTCT GGTTCTGAGC CCATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAACT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAATTTG CTGAAACCAT CGAAACAGTA 
CTGAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTCTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAATGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGCGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAGCCTCA CCCACAACTA ACAAGGAATT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATACATCTA 
TTCCTGTCAC CCAGGCAGGT GCACTTGCTT TTGGACATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAATCGACC TATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGATATT ATTTGAGGAA AGATTCCCTC 
TCTGCCGGCG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGAT CAGCGTTACC CTCCAACCTA 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTTCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAGTCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAAATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTTC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCTTGT TTCCGACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGGTAGT 
CAAGCAAGAC TTAGTTCCGT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCGCAGAAA 
CTCACTACAG TGGAGATGAT GGCATCACAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGACTCAC AGAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAGCTTAC ATTTAGAGAA CTAGTTGGGT CATTCCAGGA AGATAAAGGA 
GAACCATCCA TTAAGTTTTT CCATGTGGCT AGTGGCGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTG AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGATG GCCACTATCA GGAGGAAGAG 
GAGGGAGGAG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC CCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACCG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ATTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTGCCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ATTTGCTGCT ATGGGAACCA ACAGCTCCTT CACCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCATTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TATTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTCGA GTTCAGCAGC GGCTCGTTGT TTTGTGTGAC AAAGTATGAA 
GGCTTTGACG ACAAGCACTA CGTGTGCCTT CACTGCAGCA GCTTCAGTCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATACAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCGCT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCCCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTCGCCTTGG ATAAATCATC TTCAACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCAC ACTTTGAATT GCACTGTTCG 
AGTGACGTTG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATACATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTAGA TTCTGTTGGT CGGTCATCCT CACGTGGTCC AGTACTTCCT 
GAAGCAGATC AACAGATACT ACGAGATTTG ATGAGTGATG CTATGGAGGA GATTGACATG 
CAAGAAGCAG CTTCATCTGT AAAACCACAG TCTAATGGTG TTTCCGATGA AAAATCTCAA 
GTTCAGGAAC CATGTTGTTC AGACCTCTTC CTGTTTCCTG ATGAGAGTGG AAACGTGTCC 
CAGGAGCCTG GCCCCACTTA CGCCTCATTC ACCCACCACT TGATTAGTGA TGCAGTGACA 
GGCGTGCCCA CGGAAAATGA CGACTTTTGC ATTCTTTTTG CACCAAGAGC AACTGTTCAG 
GAGAAGGAAG AAGAACCAGT TGTAAAAATA ATGGTTGATG ATGCAATTGT GATAAAAGAG 
AATTATTTTA GTCTGCCTGT TACGAAGACA GACACTAGCA AAGCCCCGTT ACACTTTCCC 
GTTCCTGTCG TTCGGTACCT GGTTAAAGAA GTCTCTCTCG TCTGGCATCT TTATGGAGGA 
AAAGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATTAG TCCCCATAGC 
TCACCTTCTC ACACACCTAC AAGACATGGA CATAATATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAGTTA AGCAAGGTAA AGTTTCAGCA TGAGGTCTAC 
CCGCCCTGCA AACCAGAATG CGAGTCTAGC CTTGTGGAGC ACCCGGTGTC CCGGCAGGTG 
TTCATCGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAATTT 
TTGTACCTGT ATTGCAGTAA AGAAATGCCT CGGAAAGCTC ATTCCAACAT GTTGACAATT 
AAAGCATTAC ACGTGCGTCC AGAGTCTGGC AGGTCCCCAC AGGAGTGCTG CCTAAGAGTA 
TCACTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCTT GAAGGATTTC 
TTCACAAGTC TTTCTGCAGA AGTAGAGCTG CTCATGACTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGGG CTGATGTCAC CTGCAGTTTG CCAAGGCATT TAAGCACCTC AAAAGAGCCA 
AATCTAGTTC TTTCTTTCCC TGGGCCAAAG CAGCCTTCCC CAAATGGCAG TGCCAATTCA 
GTGCAAGCGG TTAATGGGGA AGAAGAGAAG GACTTCTCTC CTGAAGAAGC GTTGTTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTTCCTAT TCGACTTGAT 
TATCATGGGA AACATGTATC AATGGATCAG GGTACATTAG CTGGGATTTT GATTGGTCTC 
GCCCAGTTAA ACTGCTCTGA ACTAAAGCTG AAGAGGCTTT TCTATCGGCA TGGTTTGCTA 
GGTGTGGACA AATTATTCTC ATATGCAATC ACCGAATGGC TTACTGACAT TAAGAAGAAC 
CAGCTTCCAG GAATCCTGGG AGGTGTTGGG CCTATGCATT CACTAGTACA ACTAGTGCAA 
GGCCTCAAGG ACTTGGTCTG GTTACCAATT GAGCAGTACC GAAAGGACGG TCGCATTGTC 
AGAGGATTTC AGAGAGGGGC TGCCTCCTTT GGCACCTCGA CGGCGATGGC TGCTCTCGAG 
CTCACAAACA GAATGGTTCA AACCATACAG GCAGCGGCAG AGACTGCGTA TGACATGGTG 
TCTCCAGGCA CCCTTTCTAT CGAGCCCAAG AAGGTCAGAA GGTTCCCCCA TCACCGGTTA 
GCCCACCAGC CAGTGGACCT GAGGGAAGGG GTGGCCAAGG CCTACAGTGT CGTGAAGGAG 
GGAATCGCAG ACACGGCTCA GACCATTTAT GAAACTGCCG CTCGGGAACA CGAGAGCCGG 
GGGGTGACGG GCGCCGTGGG GGAAGTCCTG CGCCAGCTCC CCCCGGCCGT GGTGAAACCT 
CTGATCGTCG CCACAGAAGC GACATCGAAC GTCCTGGGCG GCATGAGAAA CCAGATCAGA 
CCAGATGTTC GGCAGGACGA GTCGCAGAAG TGGCGCCATG GGGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_004017983.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004018032.1 Ovis aries autophagy related 2B (ATG2B), transcript variant X1, mRNA.

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CloneID	OSm14334
Clone ID Related Accession (Same CDS sequence)	XM_004017983.2
Accession Version	XM_004017983.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	autophagy-related protein 2 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 3, 2015 this sequence version replaced XM_004017983.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CTTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACG GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGAGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCGTCT GGTTCTGAGC CCATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAACT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAATTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAAGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAATGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGCGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAGCCTCA CCCACAACTA ACAAGGAATT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATACATCTA 
TTCCTGTCAC CCAGGCAGGT GCACTTGCTT TTGGACATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAATCGACC TATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGATATT ATTTGAGGAA AGATTCCCTC 
TCTGCTGGCG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGAT CAGCGTTACC CTCCAACCTA 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTTCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAGTCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAAATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTTC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCTTGT TTCCGACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGGTAGT 
CAAGCAAGAC TTAGTTCCGT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCGCAGAAA 
CTCACTACAG TGGAGATGAT GGCATCACAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGACTCAC AGAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAGCTTAC ATTTAGAGAA CTAGTTGGGT CATTCCAGGA AGATAAAGGA 
GAACCATCCA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTG AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGATG GCCACTATCA GGAGGAAGAG 
GAGGGAGGAG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC CCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACCG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTGCCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ATTTGCTGCT ATGGGAACCA ACAGCTCCTT CACCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCATTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TATTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTCGA GTTCAGCAGC GGCTCGTTGT TTTGTGTGAC AAAGTATGAA 
GGCTTTGACG ACAAGCACTA CGTGTGCCTT CACTGCAGCA GCTTCAGTCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATACAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCGCT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCCCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTTGCCTTGG ATAAATCATC TTCAACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCAC ACTTTGAATT GCACTGTTCG 
AGTGACGTTG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATACATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTGAA GGTAGATTCT GTTGGTCGGT CGTCCTCACG TGGTCCAGTA 
CTTCCTGAAG CAGATCAACA GATACTACGA GATTTGATGA GTGATGCTAT GGAGGAGATT 
GACATGCAAG AAGCAGCTTC ATCTGTAAAA CCACAGTCTA ATGGTGTTTC CGATGAAAAA 
TCTCAAGTTC AGGAACCATG TTGTTCAGAC CTCTTCCTGT TTCCTGATGA GAGTGGAAAC 
GTGTCCCAGG AGCCTGGCCC CACTTACGCC TCATTCACCC ACCACTTGAT TAGTGATGCA 
GTGACAGGCG TGCCCACGGA AAATGACGAC TTTTGCATTC TTTTTGCACC AAGAGCAACT 
GTTCAGGAGA AGGAAGAAGA ACCAGTTGTA AAAATAATGG TTGATGATGC AATTGTGATA 
AAAGACAATT ATTTTAGTCT GCCTGTTACG AAGACAGATA CTAGCAAAGC CCCGTTACAC 
TTTCCCGTTC CTGTCGTTCG GTACCTGGTT AAAGAAGTCT CTCTCGTCTG GCATCTTTAT 
GGAGGAAAAG ATTTTGGAAC AGCCCCTCCT ACTTCTCCAG CTAAAAGTTA CATTAGTCCC 
CATAGCTCAC CTTCTCACAC ACCTACAAGA CATGGACATA ATATGGTATG TGGGGGAAAA 
GGAAGAAACC ATGACTTTTT AATGGAAATA CAGTTAAGCA AGGTGAAGTT TCAGCATGAG 
GTCTACCCGC CCTGCAAACC AGAATGCGAG TCTAGCCTTG TGGAGCACCC GGTGTCCCGG 
CAGGTGTTCA TCGTGCAGGA TCTTGAAATT CGAGATCGTC TGGCAACATC ACAAATGAAT 
AAATTTTTGT ACCTGTATTG CAGTAAAGAA ATGCCTCGGA AAGCTCATTC CAACATGTTG 
ACAATTAAAG CATTACATGT GCGTCCAGAG TCTGGCAGGT CCCCACAGGA GTGCTGCCTA 
AGAGTATCAC TAATGCCACT TCGCCTCAAT ATTGACCAGG ATGCCTTGTT CTTCTTGAAG 
GATTTCTTCA CAAGTCTTTC TGCAGAAGTA GAGCTGCTCA TGACTCCAGA TCCAGAAGTT 
AAAAAGTCTC CTGGGGCTGA TGTCACCTGC AGTTTGCCAA GGCATTTAAG CACCTCAAAA 
GAGCCAAATC TAGTTCTTTC TTTCCCTGGG CCAAAGCAGC CTTCCCCAAA TGGCAGTGCC 
AATTCAGTGC AAGCGGTTAA TGGGGAAGAA GAGAAGGACT TCTCTCCTGA AGAAGCGTTG 
TTTAGTGATC AGCCCGTGTT TTTTAGAGAA TTTAGATTCA CATCAGAAGT TCCTATTCGA 
CTTGATTATC ATGGGAAACA TGTATCAATG GATCAGGGTA CATTAGCTGG GATTTTGATT 
GGTCTCGCCC AGTTAAACTG CTCTGAACTA AAGCTGAAGA GGCTTTTCTA TCGGCATGGT 
TTGCTAGGTG TGGACAAATT ATTCTCATAT GCAATCACCG AATGGCTTAC TGACATTAAG 
AAGAACCAGC TTCCAGGAAT CCTGGGAGGT GTTGGGCCTA TGCATTCACT AGTACAACTA 
GTGCAAGGCC TCAAGGACTT GGTCTGGTTA CCAATTGAGC AGTACCGAAA GGACGGTCGC 
ATTGTCAGAG GATTTCAGAG AGGGGCTGCC TCCTTTGGCA CCTCGACGGC AATGGCTGCT 
CTCGAGCTCA CAAACAGAAT GGTTCAAACC ATACAGGCAG CGGCAGAGAC TGCGTATGAC 
ATGGTGTCTC CAGGCACCCT TTCTATCGAG CCCAAGAAGG TCAGAAGGTT CCCCCATCAC 
CGGTTAGCCC ACCAGCCAGT GGACCTGAGG GAAGGGGTGG CCAAGGCCTA CAGTGTCGTG 
AAGGAGGGAA TCGCAGACAC GGCTCAGACC ATTTATGAAA CTGCCGCTCG GGAACACGAG 
AGCCGGGGGG TGACGGGCGC CGTGGGGGAA GTCCTGCGCC AGCTCCCCCC GGCCGTGGTG 
AAACCTCTGA TCGTCGCCAC AGAAGCGACA TCGAACGTCC TGGGCGGCAT GAGAAACCAG 
ATCAGACCAG ATGTTCGGCA GGACGAGTCC CAGAAGTGGC GCCATGGGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004018032.1
CDS	2..6238
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRLASGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSVSVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQGSQARLSSVPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSQNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLIAVFTDVKQDNGDLLENKHGEFWFEFSSGSLFCVTKYEGFDDKHYVCLHCSSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVKVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDAIVIKDNYFSLPVTKTDTSKAPLHFPVPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEVELLMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_004017983.2
Organism	Ovis aries(sheep)
Definition	Ovis aries autophagy related 2B (ATG2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004017983.2

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CTTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACG GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGAGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCGTCT GGTTCTGAGC CCATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAACT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAATTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAAGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAATGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGCGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAGCCTCA CCCACAACTA ACAAGGAATT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATACATCTA 
TTCCTGTCAC CCAGGCAGGT GCACTTGCTT TTGGACATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAATCGACC TATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGATATT ATTTGAGGAA AGATTCCCTC 
TCTGCTGGCG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGAT CAGCGTTACC CTCCAACCTA 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTTCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAGTCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAAATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTTC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCTTGT TTCCGACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGGTAGT 
CAAGCAAGAC TTAGTTCCGT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCGCAGAAA 
CTCACTACAG TGGAGATGAT GGCATCACAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGACTCAC AGAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAGCTTAC ATTTAGAGAA CTAGTTGGGT CATTCCAGGA AGATAAAGGA 
GAACCATCCA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTG AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGATG GCCACTATCA GGAGGAAGAG 
GAGGGAGGAG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC CCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACCG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTGCCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ATTTGCTGCT ATGGGAACCA ACAGCTCCTT CACCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCATTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TATTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTCGA GTTCAGCAGC GGCTCGTTGT TTTGTGTGAC AAAGTATGAA 
GGCTTTGACG ACAAGCACTA CGTGTGCCTT CACTGCAGCA GCTTCAGTCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATACAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCGCT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCCCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTTGCCTTGG ATAAATCATC TTCAACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCAC ACTTTGAATT GCACTGTTCG 
AGTGACGTTG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATACATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTGAA GGTAGATTCT GTTGGTCGGT CGTCCTCACG TGGTCCAGTA 
CTTCCTGAAG CAGATCAACA GATACTACGA GATTTGATGA GTGATGCTAT GGAGGAGATT 
GACATGCAAG AAGCAGCTTC ATCTGTAAAA CCACAGTCTA ATGGTGTTTC CGATGAAAAA 
TCTCAAGTTC AGGAACCATG TTGTTCAGAC CTCTTCCTGT TTCCTGATGA GAGTGGAAAC 
GTGTCCCAGG AGCCTGGCCC CACTTACGCC TCATTCACCC ACCACTTGAT TAGTGATGCA 
GTGACAGGCG TGCCCACGGA AAATGACGAC TTTTGCATTC TTTTTGCACC AAGAGCAACT 
GTTCAGGAGA AGGAAGAAGA ACCAGTTGTA AAAATAATGG TTGATGATGC AATTGTGATA 
AAAGACAATT ATTTTAGTCT GCCTGTTACG AAGACAGATA CTAGCAAAGC CCCGTTACAC 
TTTCCCGTTC CTGTCGTTCG GTACCTGGTT AAAGAAGTCT CTCTCGTCTG GCATCTTTAT 
GGAGGAAAAG ATTTTGGAAC AGCCCCTCCT ACTTCTCCAG CTAAAAGTTA CATTAGTCCC 
CATAGCTCAC CTTCTCACAC ACCTACAAGA CATGGACATA ATATGGTATG TGGGGGAAAA 
GGAAGAAACC ATGACTTTTT AATGGAAATA CAGTTAAGCA AGGTGAAGTT TCAGCATGAG 
GTCTACCCGC CCTGCAAACC AGAATGCGAG TCTAGCCTTG TGGAGCACCC GGTGTCCCGG 
CAGGTGTTCA TCGTGCAGGA TCTTGAAATT CGAGATCGTC TGGCAACATC ACAAATGAAT 
AAATTTTTGT ACCTGTATTG CAGTAAAGAA ATGCCTCGGA AAGCTCATTC CAACATGTTG 
ACAATTAAAG CATTACATGT GCGTCCAGAG TCTGGCAGGT CCCCACAGGA GTGCTGCCTA 
AGAGTATCAC TAATGCCACT TCGCCTCAAT ATTGACCAGG ATGCCTTGTT CTTCTTGAAG 
GATTTCTTCA CAAGTCTTTC TGCAGAAGTA GAGCTGCTCA TGACTCCAGA TCCAGAAGTT 
AAAAAGTCTC CTGGGGCTGA TGTCACCTGC AGTTTGCCAA GGCATTTAAG CACCTCAAAA 
GAGCCAAATC TAGTTCTTTC TTTCCCTGGG CCAAAGCAGC CTTCCCCAAA TGGCAGTGCC 
AATTCAGTGC AAGCGGTTAA TGGGGAAGAA GAGAAGGACT TCTCTCCTGA AGAAGCGTTG 
TTTAGTGATC AGCCCGTGTT TTTTAGAGAA TTTAGATTCA CATCAGAAGT TCCTATTCGA 
CTTGATTATC ATGGGAAACA TGTATCAATG GATCAGGGTA CATTAGCTGG GATTTTGATT 
GGTCTCGCCC AGTTAAACTG CTCTGAACTA AAGCTGAAGA GGCTTTTCTA TCGGCATGGT 
TTGCTAGGTG TGGACAAATT ATTCTCATAT GCAATCACCG AATGGCTTAC TGACATTAAG 
AAGAACCAGC TTCCAGGAAT CCTGGGAGGT GTTGGGCCTA TGCATTCACT AGTACAACTA 
GTGCAAGGCC TCAAGGACTT GGTCTGGTTA CCAATTGAGC AGTACCGAAA GGACGGTCGC 
ATTGTCAGAG GATTTCAGAG AGGGGCTGCC TCCTTTGGCA CCTCGACGGC AATGGCTGCT 
CTCGAGCTCA CAAACAGAAT GGTTCAAACC ATACAGGCAG CGGCAGAGAC TGCGTATGAC 
ATGGTGTCTC CAGGCACCCT TTCTATCGAG CCCAAGAAGG TCAGAAGGTT CCCCCATCAC 
CGGTTAGCCC ACCAGCCAGT GGACCTGAGG GAAGGGGTGG CCAAGGCCTA CAGTGTCGTG 
AAGGAGGGAA TCGCAGACAC GGCTCAGACC ATTTATGAAA CTGCCGCTCG GGAACACGAG 
AGCCGGGGGG TGACGGGCGC CGTGGGGGAA GTCCTGCGCC AGCTCCCCCC GGCCGTGGTG 
AAACCTCTGA TCGTCGCCAC AGAAGCGACA TCGAACGTCC TGGGCGGCAT GAGAAACCAG 
ATCAGACCAG ATGTTCGGCA GGACGAGTCC CAGAAGTGGC GCCATGGGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_012099021.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954411.1 Ovis aries autophagy related 2B (ATG2B), transcript variant X2, mRNA.

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CloneID	OSm42091
Clone ID Related Accession (Same CDS sequence)	XM_012099021.1
Accession Version	XM_012099021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	autophagy-related protein 2 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CTTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACG GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGAGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCGTCT GGTTCTGAGC CCATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAACT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAATTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAAGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAATGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGCGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAGCCTCA CCCACAACTA ACAAGGAATT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATACATCTA 
TTCCTGTCAC CCAGGCAGGT GCACTTGCTT TTGGACATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAATCGACC TATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGATATT ATTTGAGGAA AGATTCCCTC 
TCTGCTGGCG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGAT CAGCGTTACC CTCCAACCTA 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTTCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAGTCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAAATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTTC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCTTGT TTCCGACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGGTAGT 
CAAGCAAGAC TTAGTTCCGT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCGCAGAAA 
CTCACTACAG TGGAGATGAT GGCATCACAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGACTCAC AGAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAGCTTAC ATTTAGAGAA CTAGTTGGGT CATTCCAGGA AGATAAAGGA 
GAACCATCCA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTG AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGATG GCCACTATCA GGAGGAAGAG 
GAGGGAGGAG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC CCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACCG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTGCCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ATTTGCTGCT ATGGGAACCA ACAGCTCCTT CACCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCATTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TATTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTCGA GTTCAGCAGC GGCTCGTTGT TTTGTGTGAC AAAGTATGAA 
GGCTTTGACG ACAAGCACTA CGTGTGCCTT CACTGCAGCA GCTTCAGTCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATACAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCGCT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCCCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTTGCCTTGG ATAAATCATC TTCAACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCAC ACTTTGAATT GCACTGTTCG 
AGTGACGTTG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATACATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTAGA TTCTGTTGGT CGGTCGTCCT CACGTGGTCC AGTACTTCCT 
GAAGCAGATC AACAGATACT ACGAGATTTG ATGAGTGATG CTATGGAGGA GATTGACATG 
CAAGAAGCAG CTTCATCTGT AAAACCACAG TCTAATGGTG TTTCCGATGA AAAATCTCAA 
GTTCAGGAAC CATGTTGTTC AGACCTCTTC CTGTTTCCTG ATGAGAGTGG AAACGTGTCC 
CAGGAGCCTG GCCCCACTTA CGCCTCATTC ACCCACCACT TGATTAGTGA TGCAGTGACA 
GGCGTGCCCA CGGAAAATGA CGACTTTTGC ATTCTTTTTG CACCAAGAGC AACTGTTCAG 
GAGAAGGAAG AAGAACCAGT TGTAAAAATA ATGGTTGATG ATGCAATTGT GATAAAAGAC 
AATTATTTTA GTCTGCCTGT TACGAAGACA GATACTAGCA AAGCCCCGTT ACACTTTCCC 
GTTCCTGTCG TTCGGTACCT GGTTAAAGAA GTCTCTCTCG TCTGGCATCT TTATGGAGGA 
AAAGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATTAG TCCCCATAGC 
TCACCTTCTC ACACACCTAC AAGACATGGA CATAATATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAGTTA AGCAAGGTGA AGTTTCAGCA TGAGGTCTAC 
CCGCCCTGCA AACCAGAATG CGAGTCTAGC CTTGTGGAGC ACCCGGTGTC CCGGCAGGTG 
TTCATCGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAATTT 
TTGTACCTGT ATTGCAGTAA AGAAATGCCT CGGAAAGCTC ATTCCAACAT GTTGACAATT 
AAAGCATTAC ATGTGCGTCC AGAGTCTGGC AGGTCCCCAC AGGAGTGCTG CCTAAGAGTA 
TCACTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCTT GAAGGATTTC 
TTCACAAGTC TTTCTGCAGA AGTAGAGCTG CTCATGACTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGGG CTGATGTCAC CTGCAGTTTG CCAAGGCATT TAAGCACCTC AAAAGAGCCA 
AATCTAGTTC TTTCTTTCCC TGGGCCAAAG CAGCCTTCCC CAAATGGCAG TGCCAATTCA 
GTGCAAGCGG TTAATGGGGA AGAAGAGAAG GACTTCTCTC CTGAAGAAGC GTTGTTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTTCCTAT TCGACTTGAT 
TATCATGGGA AACATGTATC AATGGATCAG GGTACATTAG CTGGGATTTT GATTGGTCTC 
GCCCAGTTAA ACTGCTCTGA ACTAAAGCTG AAGAGGCTTT TCTATCGGCA TGGTTTGCTA 
GGTGTGGACA AATTATTCTC ATATGCAATC ACCGAATGGC TTACTGACAT TAAGAAGAAC 
CAGCTTCCAG GAATCCTGGG AGGTGTTGGG CCTATGCATT CACTAGTACA ACTAGTGCAA 
GGCCTCAAGG ACTTGGTCTG GTTACCAATT GAGCAGTACC GAAAGGACGG TCGCATTGTC 
AGAGGATTTC AGAGAGGGGC TGCCTCCTTT GGCACCTCGA CGGCAATGGC TGCTCTCGAG 
CTCACAAACA GAATGGTTCA AACCATACAG GCAGCGGCAG AGACTGCGTA TGACATGGTG 
TCTCCAGGCA CCCTTTCTAT CGAGCCCAAG AAGGTCAGAA GGTTCCCCCA TCACCGGTTA 
GCCCACCAGC CAGTGGACCT GAGGGAAGGG GTGGCCAAGG CCTACAGTGT CGTGAAGGAG 
GGAATCGCAG ACACGGCTCA GACCATTTAT GAAACTGCCG CTCGGGAACA CGAGAGCCGG 
GGGGTGACGG GCGCCGTGGG GGAAGTCCTG CGCCAGCTCC CCCCGGCCGT GGTGAAACCT 
CTGATCGTCG CCACAGAAGC GACATCGAAC GTCCTGGGCG GCATGAGAAA CCAGATCAGA 
CCAGATGTTC GGCAGGACGA GTCCCAGAAG TGGCGCCATG GGGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954411.1
CDS	2..6232
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRLASGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSVSVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQGSQARLSSVPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSQNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLIAVFTDVKQDNGDLLENKHGEFWFEFSSGSLFCVTKYEGFDDKHYVCLHCSSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDAIVIKDNYFSLPVTKTDTSKAPLHFPVPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEVELLMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_012099021.1
Organism	Ovis aries(sheep)
Definition	Ovis aries autophagy related 2B (ATG2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012099021.1

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CTTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACG GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGAGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCGTCT GGTTCTGAGC CCATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAACT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAATTTG CTGAAACCAT CGAAACAGTA 
CTAAGAAGAG TAAAAGTCAC TTTTATAGAC ACTGTCTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAATGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGCGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAGCCTCA CCCACAACTA ACAAGGAATT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATACATCTA 
TTCCTGTCAC CCAGGCAGGT GCACTTGCTT TTGGACATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAATCGACC TATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGATATT ATTTGAGGAA AGATTCCCTC 
TCTGCTGGCG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGAT CAGCGTTACC CTCCAACCTA 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTTCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAGTCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAAATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTTC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCTTGT TTCCGACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGGTAGT 
CAAGCAAGAC TTAGTTCCGT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCGCAGAAA 
CTCACTACAG TGGAGATGAT GGCATCACAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGACTCAC AGAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAGCTTAC ATTTAGAGAA CTAGTTGGGT CATTCCAGGA AGATAAAGGA 
GAACCATCCA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTG AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGATG GCCACTATCA GGAGGAAGAG 
GAGGGAGGAG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC CCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACCG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTGCCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ATTTGCTGCT ATGGGAACCA ACAGCTCCTT CACCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCATTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TATTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTCGA GTTCAGCAGC GGCTCGTTGT TTTGTGTGAC AAAGTATGAA 
GGCTTTGACG ACAAGCACTA CGTGTGCCTT CACTGCAGCA GCTTCAGTCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATACAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCGCT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCCCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTTGCCTTGG ATAAATCATC TTCAACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCAC ACTTTGAATT GCACTGTTCG 
AGTGACGTTG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATACATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTAGA TTCTGTTGGT CGGTCGTCCT CACGTGGTCC AGTACTTCCT 
GAAGCAGATC AACAGATACT ACGAGATTTG ATGAGTGATG CTATGGAGGA GATTGACATG 
CAAGAAGCAG CTTCATCTGT AAAACCACAG TCTAATGGTG TTTCCGATGA AAAATCTCAA 
GTTCAGGAAC CATGTTGTTC AGACCTCTTC CTGTTTCCTG ATGAGAGTGG AAACGTGTCC 
CAGGAGCCTG GCCCCACTTA CGCCTCATTC ACCCACCACT TGATTAGTGA TGCAGTGACA 
GGCGTGCCCA CGGAAAATGA CGACTTTTGC ATTCTTTTTG CACCAAGAGC AACTGTTCAG 
GAGAAGGAAG AAGAACCAGT TGTAAAAATA ATGGTTGATG ATGCAATTGT GATAAAAGAC 
AATTATTTTA GTCTGCCTGT TACGAAGACA GATACTAGCA AAGCCCCGTT ACACTTTCCC 
GTTCCTGTCG TTCGGTACCT GGTTAAAGAA GTCTCTCTCG TCTGGCATCT TTATGGAGGA 
AAAGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATTAG TCCCCATAGC 
TCACCTTCTC ACACACCTAC AAGACATGGA CATAATATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAGTTA AGCAAGGTGA AGTTTCAGCA TGAGGTCTAC 
CCGCCCTGCA AACCAGAATG CGAGTCTAGC CTTGTGGAGC ACCCGGTGTC CCGGCAGGTG 
TTCATCGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAATTT 
TTGTACCTGT ATTGCAGTAA AGAAATGCCT CGGAAAGCTC ATTCCAACAT GTTGACAATT 
AAAGCATTAC ATGTGCGTCC AGAGTCTGGC AGGTCCCCAC AGGAGTGCTG CCTAAGAGTA 
TCACTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCTT GAAGGATTTC 
TTCACAAGTC TTTCTGCAGA AGTAGAGCTG CTCATGACTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGGG CTGATGTCAC CTGCAGTTTG CCAAGGCATT TAAGCACCTC AAAAGAGCCA 
AATCTAGTTC TTTCTTTCCC TGGGCCAAAG CAGCCTTCCC CAAATGGCAG TGCCAATTCA 
GTGCAAGCGG TTAATGGGGA AGAAGAGAAG GACTTCTCTC CTGAAGAAGC GTTGTTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTTCCTAT TCGACTTGAT 
TATCATGGGA AACATGTATC AATGGATCAG GGTACATTAG CTGGGATTTT GATTGGTCTC 
GCCCAGTTAA ACTGCTCTGA ACTAAAGCTG AAGAGGCTTT TCTATCGGCA TGGTTTGCTA 
GGTGTGGACA AATTATTCTC ATATGCAATC ACCGAATGGC TTACTGACAT TAAGAAGAAC 
CAGCTTCCAG GAATCCTGGG AGGTGTTGGG CCTATGCATT CACTAGTACA ACTAGTGCAA 
GGCCTCAAGG ACTTGGTCTG GTTACCAATT GAGCAGTACC GAAAGGACGG TCGCATTGTC 
AGAGGATTTC AGAGAGGGGC TGCCTCCTTT GGCACCTCGA CGGCAATGGC TGCTCTCGAG 
CTCACAAACA GAATGGTTCA AACCATACAG GCAGCGGCAG AGACTGCGTA TGACATGGTG 
TCTCCAGGCA CCCTTTCTAT CGAGCCCAAG AAGGTCAGAA GGTTCCCCCA TCACCGGTTA 
GCCCACCAGC CAGTGGACCT GAGGGAAGGG GTGGCCAAGG CCTACAGTGT CGTGAAGGAG 
GGAATCGCAG ACACGGCTCA GACCATTTAT GAAACTGCCG CTCGGGAACA CGAGAGCCGG 
GGGGTGACGG GCGCCGTGGG GGAAGTCCTG CGCCAGCTCC CCCCGGCCGT GGTGAAACCT 
CTGATCGTCG CCACAGAAGC GACATCGAAC GTCCTGGGCG GCATGAGAAA CCAGATCAGA 
CCAGATGTTC GGCAGGACGA GTCCCAGAAG TGGCGCCATG GGGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_004017983.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004018032.2 Ovis aries autophagy related 2B (ATG2B), transcript variant X1, mRNA.

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CloneID	OSm68068
Clone ID Related Accession (Same CDS sequence)	XM_004017983.3
Accession Version	XM_004017983.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	autophagy-related protein 2 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040269.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_004017983.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CTTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACG GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGAGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCGTCT GGTTCTGAGC CCATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAACT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAATTTG CTGAAACCAT CGAAACAGTA 
CTGAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTCTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAATGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGCGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAGCCTCA CCCACAACTA ACAAGGAATT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATACATCTA 
TTCCTGTCAC CCAGGCAGGT GCACTTGCTT TTGGACATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAATCGACC TATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGATATT ATTTGAGGAA AGATTCCCTC 
TCTGCCGGCG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGAT CAGCGTTACC CTCCAACCTA 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTTCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAGTCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAAATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTTC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCTTGT TTCCGACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGGTAGT 
CAAGCAAGAC TTAGTTCCGT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCGCAGAAA 
CTCACTACAG TGGAGATGAT GGCATCACAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGACTCAC AGAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAGCTTAC ATTTAGAGAA CTAGTTGGGT CATTCCAGGA AGATAAAGGA 
GAACCATCCA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTG AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGATG GCCACTATCA GGAGGAAGAG 
GAGGGAGGAG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC CCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACCG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTGCCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ATTTGCTGCT ATGGGAACCA ACAGCTCCTT CACCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCATTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TGTTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTCGA GTTCAGCAGC GGCTCGTTGT TTTGTGTGAC AAAGTATGAA 
GGCTTTGACG ACAAGCACTA CGTGTGCCTT CACTGCAGCA GCTTCAGTCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATACAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCACT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCCCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTTGCCTTGG ATAAATCATC TTCAACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCAC ACTTTGAATT GCACTGTTCG 
AGTGACGTTG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATACATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTGAA GGTAGATTCT GTTGGTCGGT CATCCTCACG TGGTCCAGTA 
CTTCCTGAAG CAGATCAACA GATACTACGA GATTTGATGA GTGATGCTAT GGAGGAGATT 
GACATGCAAG AAGCAGCTTC ATCTGTAAAA CCACAGTCTA ATGGTGTTTC CGATGAAAAA 
TCTCAAGTTC AGGAACCATG TTGTTCAGAC CTCTTCCTGT TTCCTGATGA GAGTGGAAAC 
GTGTCCCAGG AGCCTGGCCC CACTTACGCC TCATTCACCC ACCACTTGAT TAGTGATGCA 
GTGACAGGCG TGCCCACGGA AAATGACGAC TTTTGCATTC TTTTTGCACC AAGAGCAACT 
GTTCAGGAGA AGGAAGAAGA ACCAGTTGTA AAAATAATGG TTGATGATGC AATTGTGATA 
AAAGACAATT ATTTTAGTCT GCCTGTTACG AAGACAGATA CTAGCAAAGC CCCGTTACAC 
TTTCCCGTTC CTGTCGTTCG ATACCTGGTT AAAGAAGTCT CTCTCGTCTG GCATCTTTAT 
GGAGGAAAAG ATTTTGGAAC AGCCCCTCCT ACTTCTCCAG CTAAAAGTTA CATTAGTCCC 
CATAGCTCAC CTTCTCACAC ACCTACAAGA CATGGACATA ATATGGTATG TGGGGGAAAA 
GGAAGAAACC ATGACTTTTT AATGGAAATA CAGTTAAGCA AGGTGAAGTT TCAGCATGAG 
GTCTACCCGC CCTGCAAACC AGAATGCGAG TCTAGCCTTG TGGAGCACCC GGTGTCCCGG 
CAGGTGTTCA TCGTGCAGGA TCTTGAAATT CGAGATCGTC TGGCAACATC ACAAATGAAT 
AAATTTTTGT ACCTGTATTG CAGTAAAGAA ATGCCTCGGA AAGCTCATTC CAACATGTTG 
ACAATTAAAG CATTACACGT GCGTCCAGAG TCTGGCAGGT CCCCACAGGA GTGCTGCCTA 
AGAGTATCAC TAATGCCACT TCGCCTCAAT ATTGACCAGG ATGCCTTGTT CTTCTTGAAG 
GATTTCTTCA CAAGTCTTTC TGCAGAAGTA GAGCTGCTCA TGACTCCAGA TCCAGAAGTT 
AAAAAGTCTC CTGGGGCTGA TGTCACCTGC AGTTTGCCAA GGCATTTAAG CACCTCAAAA 
GAGCCAAATC TAGTTCTTTC TTTCCCTGGG CCAAAGCAGC CTTCCCCAAA TGGCAGTGCC 
AATTCAGTGC AAGCGGTTAA TGGGGAAGAA GAGAAGGACT TCTCTCCTGA AGAAGCGTTG 
TTTAGTGATC AGCCCGTGTT TTTTAGAGAA TTTAGATTCA CATCAGAAGT TCCTATTCGA 
CTTGATTATC ATGGGAAACA TGTATCAATG GATCAGGGTA CATTAGCTGG GATTTTGATT 
GGTCTCGCCC AGTTAAACTG CTCTGAACTA AAGCTGAAGA GGCTTTTCTA TCGGCATGGT 
TTGCTAGGTG TGGACAAATT ATTCTCATAT GCAATCACCG AATGGCTTAC TGACATTAAG 
AAGAACCAGC TTCCAGGAAT CCTGGGAGGT GTTGGGCCTA TGCATTCACT AGTACAACTA 
GTGCAAGGCC TCAAGGACTT GGTCTGGTTA CCAATTGAGC AGTACCGAAA GGACGGTCGC 
ATTGTCAGAG GATTTCAGAG AGGGGCTGCC TCCTTTGGCA CCTCGACGGC GATGGCTGCT 
CTCGAGCTCA CAAACAGAAT GGTTCAAACC ATACAGGCAG CGGCAGAGAC TGCGTATGAC 
ATGGTGTCTC CAGGCACCCT TTCTATCGAG CCCAAGAAGG TCAGAAGGTT CCCCCATCAC 
CGGTTAGCCC ACCAGCCAGT GGACCTGAGG GAAGGGGTGG CCAAGGCCTA CAGTGTCGTG 
AAGGAGGGAA TCGCAGACAC GGCTCAGACC ATTTATGAAA CTGCCGCTCG GGAACACGAG 
AGCCGGGGGG TGACGGGCGC CGTGGGGGAA GTCCTGCGCC AGCTCCCCCC GGCCGTGGTG 
AAACCTCTGA TCGTCGCCAC AGAAGCGACA TCGAACGTCC TGGGCGGCAT GAGAAACCAG 
ATCAGACCAG ATGTTCGGCA GGACGAGTCG CAGAAGTGGC GCCATGGGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004018032.2
CDS	458..6694
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRLASGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSVSVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQGSQARLSSVPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSQNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSVNHGLIAVFTDVKQDNGDLLENKHGEFWFEFSSGSLFCVTKYEGFDDKHYVCLHCSSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVKVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDAIVIKDNYFSLPVTKTDTSKAPLHFPVPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEVELLMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_004017983.3
Organism	Ovis aries(sheep)
Definition	Ovis aries autophagy related 2B (ATG2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004017983.3

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CTTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACG GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGAGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCGTCT GGTTCTGAGC CCATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAACT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAATTTG CTGAAACCAT CGAAACAGTA 
CTGAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTCTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAATGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGCGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAGCCTCA CCCACAACTA ACAAGGAATT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATACATCTA 
TTCCTGTCAC CCAGGCAGGT GCACTTGCTT TTGGACATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAATCGACC TATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGATATT ATTTGAGGAA AGATTCCCTC 
TCTGCCGGCG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGAT CAGCGTTACC CTCCAACCTA 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTTCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAGTCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAAATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTTC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCTTGT TTCCGACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGGTAGT 
CAAGCAAGAC TTAGTTCCGT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCGCAGAAA 
CTCACTACAG TGGAGATGAT GGCATCACAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGACTCAC AGAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAGCTTAC ATTTAGAGAA CTAGTTGGGT CATTCCAGGA AGATAAAGGA 
GAACCATCCA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTG AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGATG GCCACTATCA GGAGGAAGAG 
GAGGGAGGAG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC CCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACCG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTGCCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ATTTGCTGCT ATGGGAACCA ACAGCTCCTT CACCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCATTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TGTTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTCGA GTTCAGCAGC GGCTCGTTGT TTTGTGTGAC AAAGTATGAA 
GGCTTTGACG ACAAGCACTA CGTGTGCCTT CACTGCAGCA GCTTCAGTCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATACAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCACT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCCCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTTGCCTTGG ATAAATCATC TTCAACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCAC ACTTTGAATT GCACTGTTCG 
AGTGACGTTG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATACATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTGAA GGTAGATTCT GTTGGTCGGT CATCCTCACG TGGTCCAGTA 
CTTCCTGAAG CAGATCAACA GATACTACGA GATTTGATGA GTGATGCTAT GGAGGAGATT 
GACATGCAAG AAGCAGCTTC ATCTGTAAAA CCACAGTCTA ATGGTGTTTC CGATGAAAAA 
TCTCAAGTTC AGGAACCATG TTGTTCAGAC CTCTTCCTGT TTCCTGATGA GAGTGGAAAC 
GTGTCCCAGG AGCCTGGCCC CACTTACGCC TCATTCACCC ACCACTTGAT TAGTGATGCA 
GTGACAGGCG TGCCCACGGA AAATGACGAC TTTTGCATTC TTTTTGCACC AAGAGCAACT 
GTTCAGGAGA AGGAAGAAGA ACCAGTTGTA AAAATAATGG TTGATGATGC AATTGTGATA 
AAAGACAATT ATTTTAGTCT GCCTGTTACG AAGACAGATA CTAGCAAAGC CCCGTTACAC 
TTTCCCGTTC CTGTCGTTCG ATACCTGGTT AAAGAAGTCT CTCTCGTCTG GCATCTTTAT 
GGAGGAAAAG ATTTTGGAAC AGCCCCTCCT ACTTCTCCAG CTAAAAGTTA CATTAGTCCC 
CATAGCTCAC CTTCTCACAC ACCTACAAGA CATGGACATA ATATGGTATG TGGGGGAAAA 
GGAAGAAACC ATGACTTTTT AATGGAAATA CAGTTAAGCA AGGTGAAGTT TCAGCATGAG 
GTCTACCCGC CCTGCAAACC AGAATGCGAG TCTAGCCTTG TGGAGCACCC GGTGTCCCGG 
CAGGTGTTCA TCGTGCAGGA TCTTGAAATT CGAGATCGTC TGGCAACATC ACAAATGAAT 
AAATTTTTGT ACCTGTATTG CAGTAAAGAA ATGCCTCGGA AAGCTCATTC CAACATGTTG 
ACAATTAAAG CATTACACGT GCGTCCAGAG TCTGGCAGGT CCCCACAGGA GTGCTGCCTA 
AGAGTATCAC TAATGCCACT TCGCCTCAAT ATTGACCAGG ATGCCTTGTT CTTCTTGAAG 
GATTTCTTCA CAAGTCTTTC TGCAGAAGTA GAGCTGCTCA TGACTCCAGA TCCAGAAGTT 
AAAAAGTCTC CTGGGGCTGA TGTCACCTGC AGTTTGCCAA GGCATTTAAG CACCTCAAAA 
GAGCCAAATC TAGTTCTTTC TTTCCCTGGG CCAAAGCAGC CTTCCCCAAA TGGCAGTGCC 
AATTCAGTGC AAGCGGTTAA TGGGGAAGAA GAGAAGGACT TCTCTCCTGA AGAAGCGTTG 
TTTAGTGATC AGCCCGTGTT TTTTAGAGAA TTTAGATTCA CATCAGAAGT TCCTATTCGA 
CTTGATTATC ATGGGAAACA TGTATCAATG GATCAGGGTA CATTAGCTGG GATTTTGATT 
GGTCTCGCCC AGTTAAACTG CTCTGAACTA AAGCTGAAGA GGCTTTTCTA TCGGCATGGT 
TTGCTAGGTG TGGACAAATT ATTCTCATAT GCAATCACCG AATGGCTTAC TGACATTAAG 
AAGAACCAGC TTCCAGGAAT CCTGGGAGGT GTTGGGCCTA TGCATTCACT AGTACAACTA 
GTGCAAGGCC TCAAGGACTT GGTCTGGTTA CCAATTGAGC AGTACCGAAA GGACGGTCGC 
ATTGTCAGAG GATTTCAGAG AGGGGCTGCC TCCTTTGGCA CCTCGACGGC GATGGCTGCT 
CTCGAGCTCA CAAACAGAAT GGTTCAAACC ATACAGGCAG CGGCAGAGAC TGCGTATGAC 
ATGGTGTCTC CAGGCACCCT TTCTATCGAG CCCAAGAAGG TCAGAAGGTT CCCCCATCAC 
CGGTTAGCCC ACCAGCCAGT GGACCTGAGG GAAGGGGTGG CCAAGGCCTA CAGTGTCGTG 
AAGGAGGGAA TCGCAGACAC GGCTCAGACC ATTTATGAAA CTGCCGCTCG GGAACACGAG 
AGCCGGGGGG TGACGGGCGC CGTGGGGGAA GTCCTGCGCC AGCTCCCCCC GGCCGTGGTG 
AAACCTCTGA TCGTCGCCAC AGAAGCGACA TCGAACGTCC TGGGCGGCAT GAGAAACCAG 
ATCAGACCAG ATGTTCGGCA GGACGAGTCG CAGAAGTGGC GCCATGGGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_012099021.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011954411.2 Ovis aries autophagy related 2B (ATG2B), transcript variant X2, mRNA.

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CloneID	OSm68069
Clone ID Related Accession (Same CDS sequence)	XM_012099021.2
Accession Version	XM_012099021.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	autophagy-related protein 2 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040269.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_012099021.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CTTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACG GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGAGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCGTCT GGTTCTGAGC CCATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAACT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAATTTG CTGAAACCAT CGAAACAGTA 
CTGAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTCTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAATGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGCGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAGCCTCA CCCACAACTA ACAAGGAATT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATACATCTA 
TTCCTGTCAC CCAGGCAGGT GCACTTGCTT TTGGACATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAATCGACC TATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGATATT ATTTGAGGAA AGATTCCCTC 
TCTGCCGGCG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGAT CAGCGTTACC CTCCAACCTA 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTTCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAGTCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAAATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTTC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCTTGT TTCCGACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGGTAGT 
CAAGCAAGAC TTAGTTCCGT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCGCAGAAA 
CTCACTACAG TGGAGATGAT GGCATCACAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGACTCAC AGAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAGCTTAC ATTTAGAGAA CTAGTTGGGT CATTCCAGGA AGATAAAGGA 
GAACCATCCA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTG AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGATG GCCACTATCA GGAGGAAGAG 
GAGGGAGGAG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC CCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACCG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTGCCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ATTTGCTGCT ATGGGAACCA ACAGCTCCTT CACCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCATTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TGTTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTCGA GTTCAGCAGC GGCTCGTTGT TTTGTGTGAC AAAGTATGAA 
GGCTTTGACG ACAAGCACTA CGTGTGCCTT CACTGCAGCA GCTTCAGTCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATACAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCACT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCCCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTTGCCTTGG ATAAATCATC TTCAACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCAC ACTTTGAATT GCACTGTTCG 
AGTGACGTTG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATACATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTAGA TTCTGTTGGT CGGTCATCCT CACGTGGTCC AGTACTTCCT 
GAAGCAGATC AACAGATACT ACGAGATTTG ATGAGTGATG CTATGGAGGA GATTGACATG 
CAAGAAGCAG CTTCATCTGT AAAACCACAG TCTAATGGTG TTTCCGATGA AAAATCTCAA 
GTTCAGGAAC CATGTTGTTC AGACCTCTTC CTGTTTCCTG ATGAGAGTGG AAACGTGTCC 
CAGGAGCCTG GCCCCACTTA CGCCTCATTC ACCCACCACT TGATTAGTGA TGCAGTGACA 
GGCGTGCCCA CGGAAAATGA CGACTTTTGC ATTCTTTTTG CACCAAGAGC AACTGTTCAG 
GAGAAGGAAG AAGAACCAGT TGTAAAAATA ATGGTTGATG ATGCAATTGT GATAAAAGAC 
AATTATTTTA GTCTGCCTGT TACGAAGACA GATACTAGCA AAGCCCCGTT ACACTTTCCC 
GTTCCTGTCG TTCGATACCT GGTTAAAGAA GTCTCTCTCG TCTGGCATCT TTATGGAGGA 
AAAGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATTAG TCCCCATAGC 
TCACCTTCTC ACACACCTAC AAGACATGGA CATAATATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAGTTA AGCAAGGTGA AGTTTCAGCA TGAGGTCTAC 
CCGCCCTGCA AACCAGAATG CGAGTCTAGC CTTGTGGAGC ACCCGGTGTC CCGGCAGGTG 
TTCATCGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAATTT 
TTGTACCTGT ATTGCAGTAA AGAAATGCCT CGGAAAGCTC ATTCCAACAT GTTGACAATT 
AAAGCATTAC ACGTGCGTCC AGAGTCTGGC AGGTCCCCAC AGGAGTGCTG CCTAAGAGTA 
TCACTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCTT GAAGGATTTC 
TTCACAAGTC TTTCTGCAGA AGTAGAGCTG CTCATGACTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGGG CTGATGTCAC CTGCAGTTTG CCAAGGCATT TAAGCACCTC AAAAGAGCCA 
AATCTAGTTC TTTCTTTCCC TGGGCCAAAG CAGCCTTCCC CAAATGGCAG TGCCAATTCA 
GTGCAAGCGG TTAATGGGGA AGAAGAGAAG GACTTCTCTC CTGAAGAAGC GTTGTTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTTCCTAT TCGACTTGAT 
TATCATGGGA AACATGTATC AATGGATCAG GGTACATTAG CTGGGATTTT GATTGGTCTC 
GCCCAGTTAA ACTGCTCTGA ACTAAAGCTG AAGAGGCTTT TCTATCGGCA TGGTTTGCTA 
GGTGTGGACA AATTATTCTC ATATGCAATC ACCGAATGGC TTACTGACAT TAAGAAGAAC 
CAGCTTCCAG GAATCCTGGG AGGTGTTGGG CCTATGCATT CACTAGTACA ACTAGTGCAA 
GGCCTCAAGG ACTTGGTCTG GTTACCAATT GAGCAGTACC GAAAGGACGG TCGCATTGTC 
AGAGGATTTC AGAGAGGGGC TGCCTCCTTT GGCACCTCGA CGGCGATGGC TGCTCTCGAG 
CTCACAAACA GAATGGTTCA AACCATACAG GCAGCGGCAG AGACTGCGTA TGACATGGTG 
TCTCCAGGCA CCCTTTCTAT CGAGCCCAAG AAGGTCAGAA GGTTCCCCCA TCACCGGTTA 
GCCCACCAGC CAGTGGACCT GAGGGAAGGG GTGGCCAAGG CCTACAGTGT CGTGAAGGAG 
GGAATCGCAG ACACGGCTCA GACCATTTAT GAAACTGCCG CTCGGGAACA CGAGAGCCGG 
GGGGTGACGG GCGCCGTGGG GGAAGTCCTG CGCCAGCTCC CCCCGGCCGT GGTGAAACCT 
CTGATCGTCG CCACAGAAGC GACATCGAAC GTCCTGGGCG GCATGAGAAA CCAGATCAGA 
CCAGATGTTC GGCAGGACGA GTCGCAGAAG TGGCGCCATG GGGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011954411.2
CDS	458..6688
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRLASGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTMYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLNGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSAGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGEPPNMDLELSLTSTYTNTPAGSPLSTTVLQPTWGDFLDHHKEQPVRGSALPSNLVHPASLQKTALPSRSVSVDESRPELIFRVAVGTFSVSVLHIDPLSPPESSLNVNPLTPMAIAFFTCIEKIDPAQFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDSHSVPPHYTELLMFHSKERTDSHLPVCLQLHYKHSENKGPQGSQARLSSVPQKAELQVKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSQNPANCRVSVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILHLAFTDLEFKTEFLGGSTPEQVKLELTFRELVGSFQEDKGEPSIKFFHVASGGDGDTTSSDDFDWPRIVLKVNPPAMHSILERIAAEEEEESDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQNKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFSKDSFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSVNHGLIAVFTDVKQDNGDLLENKHGEFWFEFSSGSLFCVTKYEGFDDKHYVCLHCSSFSLYHKGIVDGAVPSTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKIVSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLNRDYVRVMDMGLLELTITAVKSDSDGEQTEPHFELHCSSDVVHVRTCSDSCAALMNLIQYIASYGDLHATNKADVKPGAPQRKVDSVGRSSSRGPVLPEADQQILRDLMSDAMEEIDMQEAASSVKPQSNGVSDEKSQVQEPCCSDLFLFPDESGNVSQEPGPTYASFTHHLISDAVTGVPTENDDFCILFAPRATVQEKEEEPVVKIMVDDAIVIKDNYFSLPVTKTDTSKAPLHFPVPVVRYLVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGHNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECESSLVEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTIKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEVELLMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVLSFPGPKQPSPNGSANSVQAVNGEEEKDFSPEEALFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKVRRFPHHRLAHQPVDLREGVAKAYSVVKEGIADTAQTIYETAAREHESRGVTGAVGEVLRQLPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_012099021.2
Organism	Ovis aries(sheep)
Definition	Ovis aries autophagy related 2B (ATG2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012099021.2

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GTCGGTACCT CTTGCAGAGG 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GGTCCCTCGC TCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCAGCAG ATGCACCTTT AGAAGTCACG GAAGGATTCA TACAGTCAAT TTCTCTGTCC 
GTTCCATGGG GCTCCTTGTT GCAGGACAAT TGTGCACTGG AAGTGAGAGG GTTAGAAATG 
GTCTTCCGAC CTCGACCCCG CCTAGCGTCT GGTTCTGAGC CCATGTACTG GTCAAGCTTC 
ATGACCAGCA GTATGCAACT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAATTTG CTGAAACCAT CGAAACAGTA 
CTGAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTCTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACCATGTA CTGTGATGAA 
ACTGCTGACG AGTCCTCAGG AATTAATGTA CATCAACCCA CAGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCTGC AAAATCCTCC 
CCAGTGTGTT CAACTGCGCC AGTGGAAACA GAGCCAAAGC TGTCACCTAG CTGGAATCCA 
AAAATTGTTT ATGAGCCTCA CCCACAACTA ACAAGGAATT TGCCAGAAGT AGCACCTTCT 
GACCCAGTGC AGATAGGAGG GCTCAATGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATACATCTA 
TTCCTGTCAC CCAGGCAGGT GCACTTGCTT TTGGACATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCAA GCAAAATTGG ATTAGCTAAT AAAGATAGAA AAAATCGACC TATGCAGCAA 
GAAGATGAAT ATCGAATTCA AATGGAATTG AACCGATATT ATTTGAGGAA AGATTCCCTC 
TCTGCCGGCG TGTCCTCAGA GCAAAGCTTT TATGAGACAG AAACGGCTCG TACACCTTCT 
AGCCGGGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCGCA CAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA ACCCCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAACA CCCCAGCAGG ATCTCCATTA AGTACTACTG TGCTTCAGCC AACATGGGGA 
GACTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGAT CAGCGTTACC CTCCAACCTA 
GTTCACCCAG CATCTTTACA AAAGACTGCT CTTCCCTCTA GGTCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGAGTAGCT GTGGGAACGT TTTCAGTCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAATCATCA CTGAATGTTA ATCCATTGAC ACCAATGGCG 
ATAGCTTTCT TTACTTGTAT AGAAAAAATT GATCCAGCAC AATTTTCAAC AGAAGATTTT 
AAGTCTTTTC GAGCAGTATT TGCAGAAGCT TGCTCACATG ATCACCTTAG ATTTATAGGC 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTCAGTACT 
GATATGTCCA TTGGGCAAAT GGAACTTTTG GAATGCTTGT TTCCGACTGA TTCTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTGATG TTCCATTCCA AAGAACGAAC TGATTCTCAC 
CTCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC TCAGGGTAGT 
CAAGCAAGAC TTAGTTCCGT TCCTCAGAAG GCAGAATTAC AGGTTAAGTT AAATCCAGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCGCAGAAA 
CTCACTACAG TGGAGATGAT GGCATCACAT ATGTATACTT CATATAATAA GCATATTAGT 
TTGCACAAAG CTTTCACTGA AGTATTTCTA GATGACTCAC AGAATCCTGC AAACTGCCGG 
GTATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCAG ATCAAGAGAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATTCTT 
CATTTAGCAT TCACAGATCT AGAGTTTAAA ACTGAATTCC TAGGAGGATC AACCCCTGAA 
CAAGTTAAAT TGGAGCTTAC ATTTAGAGAA CTAGTTGGGT CATTCCAGGA AGATAAAGGA 
GAACCATCCA TTAAGTTTTT CCATGTGGCT AGTGGAGGAG ATGGAGATAC AACGTCATCA 
GATGACTTTG ACTGGCCGCG AATTGTACTG AAAGTGAACC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TAGCAGCTGA GGAGGAAGAG GAAAGTGATG GCCACTATCA GGAGGAAGAG 
GAGGGAGGAG CTCATTCCCT GAAGGATGTC TGTGATCTGA GAAGGCCAGC CCCGTCTCCC 
TTCTCTTCCC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACCG AGTTTCAGAA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACA 
TTACCCAATA TTCATATAAC ACTGCCTAAT AAAAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ATTTGCTGCT ATGGGAACCA ACAGCTCCTT CACCAGTAGA GACATTTGAA 
AATATTTCGT ATGGTATTGG GCTTTCAGTA GCCAGTCAGC TGATTAATAC CTTCAGCAAG 
GATAGTTTTA GTCCATTTAA ATCTACAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC TACTGTTGAT CCCAATTATC GTTCTCGTAG GAAAAAAAAA 
CTAGACTCCC AGAACAAGAA CTCACAGAGT TTTCTCTCAG TTCTTCTGAG TGTTAATCAT 
GGGTTAATTG CAGTGTTTAC AGATGTAAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTCGA GTTCAGCAGC GGCTCGTTGT TTTGTGTGAC AAAGTATGAA 
GGCTTTGACG ACAAGCACTA CGTGTGCCTT CACTGCAGCA GCTTCAGTCT CTACCACAAA 
GGCATAGTGG ATGGAGCAGT TCCTTCTACA GAAACACGAC TGCCTAGTTC AACCCGCCCA 
CATTGGTTAG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTCG AGCTTCTTCA 
GATGGTGTTG GAGGAGACAC TTTGAATATG CTGTCTGTTG CAGTTAAAAT AGTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAGTTTCTC ATTGCTGTAG GGCTAAAAGG AGCTACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTCT GGGATACAAT CCTCCAACTT CATTTACAAC CTTTCATGTT 
CATCTCTGGA GCTGTGCACT TGATTATAGG CCTCTTTATT TGCCAATCCG ATCCCTTCTT 
ACTGTTGAAA CATTCAGCGT TTCAAGTAGT GTTGCCTTGG ATAAATCATC TTCAACTCTC 
AGAATAATTT TGGATGAAGC TGCTTTACAC CTGTCTGACA AGTGTAATAC TGTTACTATA 
AATTTGAATA GAGATTATGT TCGTGTGATG GATATGGGAC TTTTGGAATT AACCATAACT 
GCAGTAAAGT CTGATTCCGA TGGAGAGCAA ACTGAACCAC ACTTTGAATT GCACTGTTCG 
AGTGACGTTG TCCATGTTAG AACGTGCTCA GACTCTTGTG CTGCTCTAAT GAATCTAATT 
CAATACATTG CAAGCTATGG GGACTTACAT GCCACTAACA AGGCAGACGT GAAGCCAGGA 
GCTCCTCAAA GAAAGGTAGA TTCTGTTGGT CGGTCATCCT CACGTGGTCC AGTACTTCCT 
GAAGCAGATC AACAGATACT ACGAGATTTG ATGAGTGATG CTATGGAGGA GATTGACATG 
CAAGAAGCAG CTTCATCTGT AAAACCACAG TCTAATGGTG TTTCCGATGA AAAATCTCAA 
GTTCAGGAAC CATGTTGTTC AGACCTCTTC CTGTTTCCTG ATGAGAGTGG AAACGTGTCC 
CAGGAGCCTG GCCCCACTTA CGCCTCATTC ACCCACCACT TGATTAGTGA TGCAGTGACA 
GGCGTGCCCA CGGAAAATGA CGACTTTTGC ATTCTTTTTG CACCAAGAGC AACTGTTCAG 
GAGAAGGAAG AAGAACCAGT TGTAAAAATA ATGGTTGATG ATGCAATTGT GATAAAAGAC 
AATTATTTTA GTCTGCCTGT TACGAAGACA GATACTAGCA AAGCCCCGTT ACACTTTCCC 
GTTCCTGTCG TTCGATACCT GGTTAAAGAA GTCTCTCTCG TCTGGCATCT TTATGGAGGA 
AAAGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATTAG TCCCCATAGC 
TCACCTTCTC ACACACCTAC AAGACATGGA CATAATATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAGTTA AGCAAGGTGA AGTTTCAGCA TGAGGTCTAC 
CCGCCCTGCA AACCAGAATG CGAGTCTAGC CTTGTGGAGC ACCCGGTGTC CCGGCAGGTG 
TTCATCGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAATTT 
TTGTACCTGT ATTGCAGTAA AGAAATGCCT CGGAAAGCTC ATTCCAACAT GTTGACAATT 
AAAGCATTAC ACGTGCGTCC AGAGTCTGGC AGGTCCCCAC AGGAGTGCTG CCTAAGAGTA 
TCACTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCTT GAAGGATTTC 
TTCACAAGTC TTTCTGCAGA AGTAGAGCTG CTCATGACTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGGG CTGATGTCAC CTGCAGTTTG CCAAGGCATT TAAGCACCTC AAAAGAGCCA 
AATCTAGTTC TTTCTTTCCC TGGGCCAAAG CAGCCTTCCC CAAATGGCAG TGCCAATTCA 
GTGCAAGCGG TTAATGGGGA AGAAGAGAAG GACTTCTCTC CTGAAGAAGC GTTGTTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTTCCTAT TCGACTTGAT 
TATCATGGGA AACATGTATC AATGGATCAG GGTACATTAG CTGGGATTTT GATTGGTCTC 
GCCCAGTTAA ACTGCTCTGA ACTAAAGCTG AAGAGGCTTT TCTATCGGCA TGGTTTGCTA 
GGTGTGGACA AATTATTCTC ATATGCAATC ACCGAATGGC TTACTGACAT TAAGAAGAAC 
CAGCTTCCAG GAATCCTGGG AGGTGTTGGG CCTATGCATT CACTAGTACA ACTAGTGCAA 
GGCCTCAAGG ACTTGGTCTG GTTACCAATT GAGCAGTACC GAAAGGACGG TCGCATTGTC 
AGAGGATTTC AGAGAGGGGC TGCCTCCTTT GGCACCTCGA CGGCGATGGC TGCTCTCGAG 
CTCACAAACA GAATGGTTCA AACCATACAG GCAGCGGCAG AGACTGCGTA TGACATGGTG 
TCTCCAGGCA CCCTTTCTAT CGAGCCCAAG AAGGTCAGAA GGTTCCCCCA TCACCGGTTA 
GCCCACCAGC CAGTGGACCT GAGGGAAGGG GTGGCCAAGG CCTACAGTGT CGTGAAGGAG 
GGAATCGCAG ACACGGCTCA GACCATTTAT GAAACTGCCG CTCGGGAACA CGAGAGCCGG 
GGGGTGACGG GCGCCGTGGG GGAAGTCCTG CGCCAGCTCC CCCCGGCCGT GGTGAAACCT 
CTGATCGTCG CCACAGAAGC GACATCGAAC GTCCTGGGCG GCATGAGAAA CCAGATCAGA 
CCAGATGTTC GGCAGGACGA GTCGCAGAAG TGGCGCCATG GGGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ATG2B cDNA ORF clone, Ovis aries(sheep)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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