PAPPA2 cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	PAPPA2
Entrez Gene ID	101118205
Full Name	pappalysin 2
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012165659.2	XP_012021049.1	pappalysin-2 isoform X2
XM_012165660.2	XP_012021050.1	pappalysin-2 isoform X2
XM_004013823.3	XP_004013872.1	pappalysin-2 isoform X1
XM_004013823.4	XP_004013872.2	pappalysin-2
XM_012187595.2	XP_012042985.1	pappalysin-2 isoform X1

Pathways from BioSystems
Gene Ontology

The following PAPPA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PAPPA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv35975	XM_012165660.2 Latest version!	Ovis aries musimon pappalysin 2 (PAPPA2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv35975	XM_012165659.2 Latest version!	Ovis aries musimon pappalysin 2 (PAPPA2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm10754	XM_012187595.2 Latest version!	Ovis aries pappalysin 2 (PAPPA2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm10754	XM_004013823.3 Latest version!	Ovis aries pappalysin 2 (PAPPA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm61874	XM_004013823.4 Latest version!	Ovis aries pappalysin 2 (PAPPA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PAPPA2 ( XM_012165659.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012021049.1 Ovis aries musimon pappalysin 2 (PAPPA2), transcript variant X3, mRNA.

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CloneID	OOv35975
Clone ID Related Accession (Same CDS sequence)	XM_012165659.2 , XM_012165660.2
Accession Version	XM_012165659.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	pappalysin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943164.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012165659.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCT TCAAGATTCT GAGGGTAAGC CTGGTAGTCC TGGCTGGGTG GGCGCTCAGT 
ACTGTCAGCT CGGAGCTGGG CTGGACGCGC AAGAGATCCT TGGCCCAAAG AGGGTACCTG 
AACCAGGTGC TGTCGGAAGG AGAGCGCTGT TGGCTGGGGG CCAAGGTTCG AAGGCCCAGA 
GTTGCTCCTC AGCATCACCT CTTTGGGGTC TATCCCAGCC TGCCTGGGGA CTACCTGAGG 
CCCTACCCTG CAGGAGATCA GGGAAGGGCC CATGTAAGAT GCAGTGAGCC GGATGCCGGG 
GGAATGGTTG TGAGCTCTGT GCCCCCAGAC CCAACTGCAA GTGCAAGAAA GAGGAGTGAG 
GCTGAGCAGC CAGCTGCTCC GTGGGGAGGG GACAGTACTA TTGGGCAGTC GGAGCTACTG 
GGAGATGATG ACACTTATCT TGGTGATGGA GGATCCAAGG AGCCTCTAGG TGAGGCTGGG 
ACTCCAGAAG GCACAGCCTT GGCTGCCACT GTGATCACCA GCTTCCCATG CCCAAAGGAA 
TCCGGACCAG ACACCCAAAG GAAGGGCCGG ACCAAGACCA GGCGTCGGCG CCAGGTGGTG 
AAGGGACAGG CAGGAGGCCT GCGGGGAGAC CCTGAGTTTG CGCCCCAGCA CTTCCAGCCG 
TGGCCTAAGC ATCCCCTCAC GCCTAGGGAT CGAACCGGCC CATCAGAGGG TGGCATCCGG 
AATGGTGGAG GGGACCCCTC CTGGGAAGCA GAGACCGAGA CTGTGAGCCC CCAAGGAGGA 
CTGCCTGTCT TGTACTTCTC TGGGAGGCGG GAGCCGCTGC TGCTGCGTCC AGAAGTGCTG 
GCTGACATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAAGCCGGA GGGAGGACAG 
AACAGCCCAG CCATCATCGC AGGTGTGTTT GATAACTGCT CCCACACTAT CAGTGACAAG 
GGCTGGGCCC TGGGGATCCG CTCGGGGAAG GATGTGGGAA GGCGAGATGC TCGCTTCTTC 
TTCTCTCTTC GCACCGACCG AGTGAAGAAA GCCACCACTG TGATGGGCCA CAGTCGCTAC 
CAACCGGGCA AGTGGACACA TCTGGCAGCC ACTTACGATG GACAGCGCAT GGCCTTGTAT 
GTGGATGGCA CTCAGGTGGC TAGTAGTCGA GATCAGTCTG GTCCCTTGAA CAGCCCCTTC 
ATGGCATCTT GTCGCTCTCT CCTCCTTGGG GGGGACAGCT CGGAGGATGG GCACTCTTTC 
CGTGGACACC TGGGCACCCT GGTCTTCTGG TCGACAGCCC GCCCACAAAG CCACCTTCAG 
CACAGACCTC AGCAGCCACT GGAGGTGAAG GATTTGACCA CCCTGCTACT GACCGCCAGT 
TTTGAGCCCC TGGAAGAACA GTGGGTCCCC TTTAGAGATG GGAAGTACCC ACGGCGTGAG 
GTTCTCCAGG GCTTTGAGTC AAAGCCTGAG ATTCTGTCCC CTCTGCAGCC CCCGCTCTGT 
GGGCAGACAG CATGTGACAA TGTGGAATTG ATCTCTCAGT ACAACGGGCA CTGGCCCCTC 
CGGGGAGAGA AGGTGATCCG CTACCGGGTG GTGAACATCT ATGACGACAA GGGTCTGAAC 
CCCACAGTGA GTGAGGAGCA GATCCGTCGG CAGCACGAGG TGCTCAATGA GGCCTTCAGC 
CGCTACAACA TCAGCTGGCA GCTGAGCGTC CACCGCGTCC ACAACTCCAC CCTGCGCCAC 
CGCATTGTGC TGGTGAACTG TGAGCCCAGC AAAATCGGCA ACGATCACTG TGACCCCGAG 
TGCGAGCATC CACTCACGGG CTATGACGGG GGAGACTGCC GCCTGCAGGG CCGGTGCTAC 
TCCTGGAACC GCAAGGACGG GCTCTGCCAC GTGGGGTGCA ACAACATGCT GAATGACTTT 
GACGAGGGCG ACTGCTGTGA CCCAGACGTG ACCGAGGTGC GCAAGACCTG CTTCGACCCT 
GACTCACCCA GGAGGGCATA CATGAGTGTG AAGGAGCTGA AGGAGGCACT GCAGCTCAAC 
AGCACCCACT TCCTCAATGT CTACTTTGCC AGCTCAGTAC GAGAAAACCT TGCAGGTGCT 
GCCACCTGGC CTTGGGACAA GGAAGCCATC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
GCCTATTATG GCATGCCTGG CCACACCAAC ATCATGATCC ATGAGGTGGG GCATGTCCTG 
GGACTCTACC ATGTCTTCAA AGGGGTCAGT GAGAGAGAAT CCTGCGATGA CCCCTGCAGG 
GAGACTGTGC CGTCCATGGA GACAGGAGAC CTCTGCGCCG ACACTGCCCC TACTCCCAAG 
AGTAAGCTGT GCCAGAACCC AGAGCCTGCC AATGACACCT GTGGCTTCAC CTACTTCCCA 
GGGGCTCCAT TCAACAACTA CATGAGTTAT ACAGACGATG ACTGCACTGA CAGCTTCACC 
CCCAACCAAG TGGCACGAAT GCACTGCTAT CTGGACCTTG TGTATCAGCA GTGGAGCCAG 
AGCCAGAAGC CCACCCCCAT CCCCATCCCC CCCATGGTCA TCGGGCAGAC TCAGAAGTCC 
CTCACTATCC ACTGGCTGCC TCCGATTAGT GGAGTTATGT ATGACAGGGT CCCGGGCAGC 
ATGTGTGGCG CCTGCACTGA AGATGGGACG TTCCGTCAGT ACGTGCACAC AGCTTCCTCC 
CAGCGCGTGT GTGACTCCTC AGGTTACTGG ACTCCGGAGG AGGCTGTGGG GCCTCCGGAC 
GTGGATCAGC CTTGTGAGCC GAGCTTGCAG GCCTGGAGTC CCGAGCTCCA CCTGCACCAC 
ACGAACATGA CGGTCCCCTG CCCTGCGGAA GGCTGCAGCC TGGAGCTGCT CTTTCAGCAC 
CCAGTCCAAG CGGACACTCT CACCCTGTGG GTCACCTACC TCTCCATGGG CTCTTCCCCG 
GTGCTCTTTG ACACAGAGAT CTTGCTGGAA CACCAGAAGT CTGTGCACTT GGGCCCCTTG 
GACACTTTCT GTGACACACC GCTCACTATC AAACTGCATG TTGATGGAAA GGTCATGGGG 
GTGAAAGTCT ACACCTTTGA TGAGCGGATG GAGATTGACG CAGCTCTCCT GACTTCTCAG 
CCCCACAGTC CCCTGTGTTC TGGCTGCAGG CCTGTGAGCT ACCAGATTCT GCGCGAGCCT 
CCGTTTGCCA GCGGCTTGCC TGCAGCGGTG ATGCATCCTC ACCGGAAGTT CACAGACATG 
GAGGTCATGC CTGGGCAGAT GTATCGGTAC CAAGTTCAAG CTGCAGCTGA AGCAGAACTG 
GGAGAAGCTT CGCCTCCTCT GAGCCACATC CATGGAGCTC CTTACTGTGG AGATGGGAAG 
GTTGCAAGGA ACCTGGGAGA AGAGTGTGAT GATGGAGACC TTTTGAATGG AGACGGCTGC 
TCAAGGGCAT GTGAGTTAGA GGAAGGTTTC AACTGTGTAG GGGAACCAAG CCTGTGCTAT 
ATACACGAGG GAGATGGAAT ATGTGAGCCT TTTGAGAAGG AAACCAGCAT TGTAGACTGT 
GGCCTCTATA CTCCCAAAGG ATACCTGGAT CAGTGGGCTA CCCAAGCTTT TGCTTCTCAT 
GAAGACACGA AGAAATGTCC TGTTTCCTTG GTAACTGGAG CACCTCATTC CATGGTTTGC 
ACATCATACC GTCCAGATTT ACCAGAGCAC CGACCCCTAA CTGGCTGGTT TCCCTGTGTC 
CCCAGTGGAA ATAGATCTCA AGATGAAGGG AGTAAGCAGC CAAAAGGTAG CCTGAAGAGA 
GAGGATGAGG TTTGGCTCAA AGTTTGTTTC AGTAGACCAG GAGTGGCCAC AGCAATTTTC 
ATTTTCTTGA CATCTGATGG CCTGGTATCT GGGAAACGTC AGCGGCCAAC AGTGACCCTT 
TACCTGACCG ATGTCAGTGG AAACAACCAC TCTCTTGGAA CCTATGAACT GTCATGCCAG 
CATAACCCGC TGGTTATCAA CATAACTCAT CACTCAAATG TCCTCTTCCA CCATGCCACA 
TCAGTGCTGC TGAATTTCTC AACCCCGTTG GTGGGCATCT CAGCGGTGGC TCTAAGGACA 
TCCTCCGACA CAAGTCCTTC AGCTCCCAAC AGCTGCATCA TCCCAGAGCA AAAGGGGCAA 
AATCATCAGG GACAAAGCTG TGTCCATCGG CCCTGTGGGG AGCAGGGCAG CTGTGTGCCA 
TTGCTGCTTG ATCACGCGGA TATGGTGAAC TGTACCTCTG GTGGCCCGGG TCTCATGAAG 
TGCTCTATCA CCTGTCAGAG GGGGTCTGCC CTTCAGGCCA GCAATGGGCA GTACCTCAGG 
CCCATGCAGA AGAAAATTCT GCTCACATGT TCCTCTGGGC ACTGGGACCG GGCTGTGAGC 
TGCCAACCCC TTGACTGTGG TGTCCCCGAC GCATCTTTGG TGAACTACGC AAATTTCTCC 
TGCTTGGAGG GAACCAACTT TCTGAAACGC TGCTCTATCT CCTGTATCCC ACCAGCCAAG 
CTTCAAGGAC TGAGCCCATG GCTGACCTGT CTTGAAGATG GGCTCTGGTC TCTCCCTGAA 
GCTTACTGCA AGCTGGAGTG TGATGCTCCT CCTGTTATCC CGAATGCCAA CCTGCTCCAG 
CCTCGCTGCC TCCAGGGGAA CCATGACGTG GGCTCTACCT GCAGATATGA ATGCAAACCG 
GGCTACTATG TGCTGGGAAG CACAGAGAAT AAAGTCAGGA ACAAGTCCCT GAAGATCCAG 
TGCCTGGAGA GTGGAATCTG GGAGCAAGGC AGCTGTGTCC CAGTGGTGTG CGAGCCCCCC 
TCCCCTGTCT TTGAAGGCAT GTATGAATGC ACCAATGGCT TTGAGCTGGA CAGCCAGTGT 
TTTATCAACT GTAACCAGGA AAGTAAAAGG CTTCCCATCC GCTGCACGAA GGAGGGACGG 
TGGAGCGAGG AGTTCAAGTT ATGTGAGAAC CTGCGAGGGG AATGCCCACC ACCCACCTCA 
GACCCCAAAT TTGTGGAGTA CAAGTGTGAA CAAGGACACG GGATAGGTGC AGTGTGTTCC 
CCATTGTGTA TCGTCCTCCC TAGTGATCCT GTAATCCTTC CTGAGAATAT CACTACTGAC 
ACTCTGGAGC ACTGGATGGA ACCCGTCAGA GTACAGAGTG TCATGTGTAC CGGACGGCGT 
CAGTGGCACC CAGACCCCCT CCTGATCCAC TGCATCCAGT CCTGCGAGGT AAGGTACTCC 
CCCTCCCTCA AACCCAGACC AGCGCTCTCT GCTCTGCTCA ACTCACAGAG ATTTGAGTCC 
TTCTGGTTCA GTATCTTGCC CTTTCTCTGC TCCGATGCCC TCTGCTCAGT CTCTTACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012021049.1
CDS	1760..7102
Translation	MMCFKILRVSLVVLAGWALSTVSSELGWTRKRSLAQRGYLNQVLSEGERCWLGAKVRRPRVAPQHHLFGVYPSLPGDYLRPYPAGDQGRAHVRCSEPDAGGMVVSSVPPDPTASARKRSEAEQPAAPWGGDSTIGQSELLGDDDTYLGDGGSKEPLGEAGTPEGTALAATVITSFPCPKESGPDTQRKGRTKTRRRRQVVKGQAGGLRGDPEFAPQHFQPWPKHPLTPRDRTGPSEGGIRNGGGDPSWEAETETVSPQGGLPVLYFSGRREPLLLRPEVLADIPREAFTVEAWVKPEGGQNSPAIIAGVFDNCSHTISDKGWALGIRSGKDVGRRDARFFFSLRTDRVKKATTVMGHSRYQPGKWTHLAATYDGQRMALYVDGTQVASSRDQSGPLNSPFMASCRSLLLGGDSSEDGHSFRGHLGTLVFWSTARPQSHLQHRPQQPLEVKDLTTLLLTASFEPLEEQWVPFRDGKYPRREVLQGFESKPEILSPLQPPLCGQTACDNVELISQYNGHWPLRGEKVIRYRVVNIYDDKGLNPTVSEEQIRRQHEVLNEAFSRYNISWQLSVHRVHNSTLRHRIVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRKDGLCHVGCNNMLNDFDEGDCCDPDVTEVRKTCFDPDSPRRAYMSVKELKEALQLNSTHFLNVYFASSVRENLAGAATWPWDKEAISHLGGVVLNPAYYGMPGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMETGDLCADTAPTPKSKLCQNPEPANDTCGFTYFPGAPFNNYMSYTDDDCTDSFTPNQVARMHCYLDLVYQQWSQSQKPTPIPIPPMVIGQTQKSLTIHWLPPISGVMYDRVPGSMCGACTEDGTFRQYVHTASSQRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLHHTNMTVPCPAEGCSLELLFQHPVQADTLTLWVTYLSMGSSPVLFDTEILLEHQKSVHLGPLDTFCDTPLTIKLHVDGKVMGVKVYTFDERMEIDAALLTSQPHSPLCSGCRPVSYQILREPPFASGLPAAVMHPHRKFTDMEVMPGQMYRYQVQAAAEAELGEASPPLSHIHGAPYCGDGKVARNLGEECDDGDLLNGDGCSRACELEEGFNCVGEPSLCYIHEGDGICEPFEKETSIVDCGLYTPKGYLDQWATQAFASHEDTKKCPVSLVTGAPHSMVCTSYRPDLPEHRPLTGWFPCVPSGNRSQDEGSKQPKGSLKREDEVWLKVCFSRPGVATAIFIFLTSDGLVSGKRQRPTVTLYLTDVSGNNHSLGTYELSCQHNPLVINITHHSNVLFHHATSVLLNFSTPLVGISAVALRTSSDTSPSAPNSCIIPEQKGQNHQGQSCVHRPCGEQGSCVPLLLDHADMVNCTSGGPGLMKCSITCQRGSALQASNGQYLRPMQKKILLTCSSGHWDRAVSCQPLDCGVPDASLVNYANFSCLEGTNFLKRCSISCIPPAKLQGLSPWLTCLEDGLWSLPEAYCKLECDAPPVIPNANLLQPRCLQGNHDVGSTCRYECKPGYYVLGSTENKVRNKSLKIQCLESGIWEQGSCVPVVCEPPSPVFEGMYECTNGFELDSQCFINCNQESKRLPIRCTKEGRWSEEFKLCENLRGECPPPTSDPKFVEYKCEQGHGIGAVCSPLCIVLPSDPVILPENITTDTLEHWMEPVRVQSVMCTGRRQWHPDPLLIHCIQSCEVRYSPSLKPRPALSALLNSQRFESFWFSILPFLCSDALCSVSYT

Target ORF information:

RefSeq Version	XM_012165659.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon pappalysin 2 (PAPPA2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012165659.2

ORF Insert Sequence:

ATGATGTGCT TCAAGATTCT GAGGGTAAGC CTGGTAGTCC TGGCTGGGTG GGCGCTCAGT 
ACTGTCAGCT CGGAGCTGGG CTGGACGCGC AAGAGATCCT TGGCCCAAAG AGGGTACCTG 
AACCAGGTGC TGTCGGAAGG AGAGCGCTGT TGGCTGGGGG CCAAGGTTCG AAGGCCCAGA 
GTTGCTCCTC AGCATCACCT CTTTGGGGTC TATCCCAGCC TGCCTGGGGA CTACCTGAGG 
CCCTACCCTG CAGGAGATCA GGGAAGGGCC CATGTAAGAT GCAGTGAGCC GGATGCCGGG 
GGAATGGTTG TGAGCTCTGT GCCCCCAGAC CCAACTGCAA GTGCAAGAAA GAGGAGTGAG 
GCTGAGCAGC CAGCTGCTCC GTGGGGAGGG GACAGTACTA TTGGGCAGTC GGAGCTACTG 
GGAGATGATG ACACTTATCT TGGTGATGGA GGATCCAAGG AGCCTCTAGG TGAGGCTGGG 
ACTCCAGAAG GCACAGCCTT GGCTGCCACT GTGATCACCA GCTTCCCATG CCCAAAGGAA 
TCCGGACCAG ACACCCAAAG GAAGGGCCGG ACCAAGACCA GGCGTCGGCG CCAGGTGGTG 
AAGGGACAGG CAGGAGGCCT GCGGGGAGAC CCTGAGTTTG CGCCCCAGCA CTTCCAGCCG 
TGGCCTAAGC ATCCCCTCAC GCCTAGGGAT CGAACCGGCC CATCAGAGGG TGGCATCCGG 
AATGGTGGAG GGGACCCCTC CTGGGAAGCA GAGACCGAGA CTGTGAGCCC CCAAGGAGGA 
CTGCCTGTCT TGTACTTCTC TGGGAGGCGG GAGCCGCTGC TGCTGCGTCC AGAAGTGCTG 
GCTGACATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAAGCCGGA GGGAGGACAG 
AACAGCCCAG CCATCATCGC AGGTGTGTTT GATAACTGCT CCCACACTAT CAGTGACAAG 
GGCTGGGCCC TGGGGATCCG CTCGGGGAAG GATGTGGGAA GGCGAGATGC TCGCTTCTTC 
TTCTCTCTTC GCACCGACCG AGTGAAGAAA GCCACCACTG TGATGGGCCA CAGTCGCTAC 
CAACCGGGCA AGTGGACACA TCTGGCAGCC ACTTACGATG GACAGCGCAT GGCCTTGTAT 
GTGGATGGCA CTCAGGTGGC TAGTAGTCGA GATCAGTCTG GTCCCTTGAA CAGCCCCTTC 
ATGGCATCTT GTCGCTCTCT CCTCCTTGGG GGGGACAGCT CGGAGGATGG GCACTCTTTC 
CGTGGACACC TGGGCACCCT GGTCTTCTGG TCGACAGCCC GCCCACAAAG CCACCTTCAG 
CACAGACCTC AGCAGCCACT GGAGGTGAAG GATTTGACCA CCCTGCTACT GACCGCCAGT 
TTTGAGCCCC TGGAAGAACA GTGGGTCCCC TTTAGAGATG GGAAGTACCC ACGGCGTGAG 
GTTCTCCAGG GCTTTGAGTC AAAGCCTGAG ATTCTGTCCC CTCTGCAGCC CCCGCTCTGT 
GGGCAGACAG CATGTGACAA TGTGGAATTG ATCTCTCAGT ACAACGGGCA CTGGCCCCTC 
CGGGGAGAGA AGGTGATCCG CTACCGGGTG GTGAACATCT ATGACGACAA GGGTCTGAAC 
CCCACAGTGA GTGAGGAGCA GATCCGTCGG CAGCACGAGG TGCTCAATGA GGCCTTCAGC 
CGCTACAACA TCAGCTGGCA GCTGAGCGTC CACCGCGTCC ACAACTCCAC CCTGCGCCAC 
CGCATTGTGC TGGTGAACTG TGAGCCCAGC AAAATCGGCA ACGATCACTG TGACCCCGAG 
TGCGAGCATC CACTCACGGG CTATGACGGG GGAGACTGCC GCCTGCAGGG CCGGTGCTAC 
TCCTGGAACC GCAAGGACGG GCTCTGCCAC GTGGGGTGCA ACAACATGCT GAATGACTTT 
GACGAGGGCG ACTGCTGTGA CCCAGACGTG ACCGAGGTGC GCAAGACCTG CTTCGACCCT 
GACTCACCCA GGAGGGCATA CATGAGTGTG AAGGAGCTGA AGGAGGCACT GCAGCTCAAC 
AGCACCCACT TCCTCAATGT CTACTTTGCC AGCTCAGTAC GAGAAAACCT TGCAGGTGCT 
GCCACCTGGC CTTGGGACAA GGAAGCCATC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
GCCTATTATG GCATGCCTGG CCACACCAAC ATCATGATCC ATGAGGTGGG GCATGTCCTG 
GGACTCTACC ATGTCTTCAA AGGGGTCAGT GAGAGAGAAT CCTGCGATGA CCCCTGCAGG 
GAGACTGTGC CGTCCATGGA GACAGGAGAC CTCTGCGCCG ACACTGCCCC TACTCCCAAG 
AGTAAGCTGT GCCAGAACCC AGAGCCTGCC AATGACACCT GTGGCTTCAC CTACTTCCCA 
GGGGCTCCAT TCAACAACTA CATGAGTTAT ACAGACGATG ACTGCACTGA CAGCTTCACC 
CCCAACCAAG TGGCACGAAT GCACTGCTAT CTGGACCTTG TGTATCAGCA GTGGAGCCAG 
AGCCAGAAGC CCACCCCCAT CCCCATCCCC CCCATGGTCA TCGGGCAGAC TCAGAAGTCC 
CTCACTATCC ACTGGCTGCC TCCGATTAGT GGAGTTATGT ATGACAGGGT CCCGGGCAGC 
ATGTGTGGCG CCTGCACTGA AGATGGGACG TTCCGTCAGT ACGTGCACAC AGCTTCCTCC 
CAGCGCGTGT GTGACTCCTC AGGTTACTGG ACTCCGGAGG AGGCTGTGGG GCCTCCGGAC 
GTGGATCAGC CTTGTGAGCC GAGCTTGCAG GCCTGGAGTC CCGAGCTCCA CCTGCACCAC 
ACGAACATGA CGGTCCCCTG CCCTGCGGAA GGCTGCAGCC TGGAGCTGCT CTTTCAGCAC 
CCAGTCCAAG CGGACACTCT CACCCTGTGG GTCACCTACC TCTCCATGGG CTCTTCCCCG 
GTGCTCTTTG ACACAGAGAT CTTGCTGGAA CACCAGAAGT CTGTGCACTT GGGCCCCTTG 
GACACTTTCT GTGACACACC GCTCACTATC AAACTGCATG TTGATGGAAA GGTCATGGGG 
GTGAAAGTCT ACACCTTTGA TGAGCGGATG GAGATTGACG CAGCTCTCCT GACTTCTCAG 
CCCCACAGTC CCCTGTGTTC TGGCTGCAGG CCTGTGAGCT ACCAGATTCT GCGCGAGCCT 
CCGTTTGCCA GCGGCTTGCC TGCAGCGGTG ATGCATCCTC ACCGGAAGTT CACAGACATG 
GAGGTCATGC CTGGGCAGAT GTATCGGTAC CAAGTTCAAG CTGCAGCTGA AGCAGAACTG 
GGAGAAGCTT CGCCTCCTCT GAGCCACATC CATGGAGCTC CTTACTGTGG AGATGGGAAG 
GTTGCAAGGA ACCTGGGAGA AGAGTGTGAT GATGGAGACC TTTTGAATGG AGACGGCTGC 
TCAAGGGCAT GTGAGTTAGA GGAAGGTTTC AACTGTGTAG GGGAACCAAG CCTGTGCTAT 
ATACACGAGG GAGATGGAAT ATGTGAGCCT TTTGAGAAGG AAACCAGCAT TGTAGACTGT 
GGCCTCTATA CTCCCAAAGG ATACCTGGAT CAGTGGGCTA CCCAAGCTTT TGCTTCTCAT 
GAAGACACGA AGAAATGTCC TGTTTCCTTG GTAACTGGAG CACCTCATTC CATGGTTTGC 
ACATCATACC GTCCAGATTT ACCAGAGCAC CGACCCCTAA CTGGCTGGTT TCCCTGTGTC 
CCCAGTGGAA ATAGATCTCA AGATGAAGGG AGTAAGCAGC CAAAAGGTAG CCTGAAGAGA 
GAGGATGAGG TTTGGCTCAA AGTTTGTTTC AGTAGACCAG GAGTGGCCAC AGCAATTTTC 
ATTTTCTTGA CATCTGATGG CCTGGTATCT GGGAAACGTC AGCGGCCAAC AGTGACCCTT 
TACCTGACCG ATGTCAGTGG AAACAACCAC TCTCTTGGAA CCTATGAACT GTCATGCCAG 
CATAACCCGC TGGTTATCAA CATAACTCAT CACTCAAATG TCCTCTTCCA CCATGCCACA 
TCAGTGCTGC TGAATTTCTC AACCCCGTTG GTGGGCATCT CAGCGGTGGC TCTAAGGACA 
TCCTCCGACA CAAGTCCTTC AGCTCCCAAC AGCTGCATCA TCCCAGAGCA AAAGGGGCAA 
AATCATCAGG GACAAAGCTG TGTCCATCGG CCCTGTGGGG AGCAGGGCAG CTGTGTGCCA 
TTGCTGCTTG ATCACGCGGA TATGGTGAAC TGTACCTCTG GTGGCCCGGG TCTCATGAAG 
TGCTCTATCA CCTGTCAGAG GGGGTCTGCC CTTCAGGCCA GCAATGGGCA GTACCTCAGG 
CCCATGCAGA AGAAAATTCT GCTCACATGT TCCTCTGGGC ACTGGGACCG GGCTGTGAGC 
TGCCAACCCC TTGACTGTGG TGTCCCCGAC GCATCTTTGG TGAACTACGC AAATTTCTCC 
TGCTTGGAGG GAACCAACTT TCTGAAACGC TGCTCTATCT CCTGTATCCC ACCAGCCAAG 
CTTCAAGGAC TGAGCCCATG GCTGACCTGT CTTGAAGATG GGCTCTGGTC TCTCCCTGAA 
GCTTACTGCA AGCTGGAGTG TGATGCTCCT CCTGTTATCC CGAATGCCAA CCTGCTCCAG 
CCTCGCTGCC TCCAGGGGAA CCATGACGTG GGCTCTACCT GCAGATATGA ATGCAAACCG 
GGCTACTATG TGCTGGGAAG CACAGAGAAT AAAGTCAGGA ACAAGTCCCT GAAGATCCAG 
TGCCTGGAGA GTGGAATCTG GGAGCAAGGC AGCTGTGTCC CAGTGGTGTG CGAGCCCCCC 
TCCCCTGTCT TTGAAGGCAT GTATGAATGC ACCAATGGCT TTGAGCTGGA CAGCCAGTGT 
TTTATCAACT GTAACCAGGA AAGTAAAAGG CTTCCCATCC GCTGCACGAA GGAGGGACGG 
TGGAGCGAGG AGTTCAAGTT ATGTGAGAAC CTGCGAGGGG AATGCCCACC ACCCACCTCA 
GACCCCAAAT TTGTGGAGTA CAAGTGTGAA CAAGGACACG GGATAGGTGC AGTGTGTTCC 
CCATTGTGTA TCGTCCTCCC TAGTGATCCT GTAATCCTTC CTGAGAATAT CACTACTGAC 
ACTCTGGAGC ACTGGATGGA ACCCGTCAGA GTACAGAGTG TCATGTGTAC CGGACGGCGT 
CAGTGGCACC CAGACCCCCT CCTGATCCAC TGCATCCAGT CCTGCGAGGT AAGGTACTCC 
CCCTCCCTCA AACCCAGACC AGCGCTCTCT GCTCTGCTCA ACTCACAGAG ATTTGAGTCC 
TTCTGGTTCA GTATCTTGCC CTTTCTCTGC TCCGATGCCC TCTGCTCAGT CTCTTACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( XM_012165660.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012021050.1 Ovis aries musimon pappalysin 2 (PAPPA2), transcript variant X4, mRNA.

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CloneID	OOv35975
Clone ID Related Accession (Same CDS sequence)	XM_012165659.2 , XM_012165660.2
Accession Version	XM_012165660.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	pappalysin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943164.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012165660.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCT TCAAGATTCT GAGGGTAAGC CTGGTAGTCC TGGCTGGGTG GGCGCTCAGT 
ACTGTCAGCT CGGAGCTGGG CTGGACGCGC AAGAGATCCT TGGCCCAAAG AGGGTACCTG 
AACCAGGTGC TGTCGGAAGG AGAGCGCTGT TGGCTGGGGG CCAAGGTTCG AAGGCCCAGA 
GTTGCTCCTC AGCATCACCT CTTTGGGGTC TATCCCAGCC TGCCTGGGGA CTACCTGAGG 
CCCTACCCTG CAGGAGATCA GGGAAGGGCC CATGTAAGAT GCAGTGAGCC GGATGCCGGG 
GGAATGGTTG TGAGCTCTGT GCCCCCAGAC CCAACTGCAA GTGCAAGAAA GAGGAGTGAG 
GCTGAGCAGC CAGCTGCTCC GTGGGGAGGG GACAGTACTA TTGGGCAGTC GGAGCTACTG 
GGAGATGATG ACACTTATCT TGGTGATGGA GGATCCAAGG AGCCTCTAGG TGAGGCTGGG 
ACTCCAGAAG GCACAGCCTT GGCTGCCACT GTGATCACCA GCTTCCCATG CCCAAAGGAA 
TCCGGACCAG ACACCCAAAG GAAGGGCCGG ACCAAGACCA GGCGTCGGCG CCAGGTGGTG 
AAGGGACAGG CAGGAGGCCT GCGGGGAGAC CCTGAGTTTG CGCCCCAGCA CTTCCAGCCG 
TGGCCTAAGC ATCCCCTCAC GCCTAGGGAT CGAACCGGCC CATCAGAGGG TGGCATCCGG 
AATGGTGGAG GGGACCCCTC CTGGGAAGCA GAGACCGAGA CTGTGAGCCC CCAAGGAGGA 
CTGCCTGTCT TGTACTTCTC TGGGAGGCGG GAGCCGCTGC TGCTGCGTCC AGAAGTGCTG 
GCTGACATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAAGCCGGA GGGAGGACAG 
AACAGCCCAG CCATCATCGC AGGTGTGTTT GATAACTGCT CCCACACTAT CAGTGACAAG 
GGCTGGGCCC TGGGGATCCG CTCGGGGAAG GATGTGGGAA GGCGAGATGC TCGCTTCTTC 
TTCTCTCTTC GCACCGACCG AGTGAAGAAA GCCACCACTG TGATGGGCCA CAGTCGCTAC 
CAACCGGGCA AGTGGACACA TCTGGCAGCC ACTTACGATG GACAGCGCAT GGCCTTGTAT 
GTGGATGGCA CTCAGGTGGC TAGTAGTCGA GATCAGTCTG GTCCCTTGAA CAGCCCCTTC 
ATGGCATCTT GTCGCTCTCT CCTCCTTGGG GGGGACAGCT CGGAGGATGG GCACTCTTTC 
CGTGGACACC TGGGCACCCT GGTCTTCTGG TCGACAGCCC GCCCACAAAG CCACCTTCAG 
CACAGACCTC AGCAGCCACT GGAGGTGAAG GATTTGACCA CCCTGCTACT GACCGCCAGT 
TTTGAGCCCC TGGAAGAACA GTGGGTCCCC TTTAGAGATG GGAAGTACCC ACGGCGTGAG 
GTTCTCCAGG GCTTTGAGTC AAAGCCTGAG ATTCTGTCCC CTCTGCAGCC CCCGCTCTGT 
GGGCAGACAG CATGTGACAA TGTGGAATTG ATCTCTCAGT ACAACGGGCA CTGGCCCCTC 
CGGGGAGAGA AGGTGATCCG CTACCGGGTG GTGAACATCT ATGACGACAA GGGTCTGAAC 
CCCACAGTGA GTGAGGAGCA GATCCGTCGG CAGCACGAGG TGCTCAATGA GGCCTTCAGC 
CGCTACAACA TCAGCTGGCA GCTGAGCGTC CACCGCGTCC ACAACTCCAC CCTGCGCCAC 
CGCATTGTGC TGGTGAACTG TGAGCCCAGC AAAATCGGCA ACGATCACTG TGACCCCGAG 
TGCGAGCATC CACTCACGGG CTATGACGGG GGAGACTGCC GCCTGCAGGG CCGGTGCTAC 
TCCTGGAACC GCAAGGACGG GCTCTGCCAC GTGGGGTGCA ACAACATGCT GAATGACTTT 
GACGAGGGCG ACTGCTGTGA CCCAGACGTG ACCGAGGTGC GCAAGACCTG CTTCGACCCT 
GACTCACCCA GGAGGGCATA CATGAGTGTG AAGGAGCTGA AGGAGGCACT GCAGCTCAAC 
AGCACCCACT TCCTCAATGT CTACTTTGCC AGCTCAGTAC GAGAAAACCT TGCAGGTGCT 
GCCACCTGGC CTTGGGACAA GGAAGCCATC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
GCCTATTATG GCATGCCTGG CCACACCAAC ATCATGATCC ATGAGGTGGG GCATGTCCTG 
GGACTCTACC ATGTCTTCAA AGGGGTCAGT GAGAGAGAAT CCTGCGATGA CCCCTGCAGG 
GAGACTGTGC CGTCCATGGA GACAGGAGAC CTCTGCGCCG ACACTGCCCC TACTCCCAAG 
AGTAAGCTGT GCCAGAACCC AGAGCCTGCC AATGACACCT GTGGCTTCAC CTACTTCCCA 
GGGGCTCCAT TCAACAACTA CATGAGTTAT ACAGACGATG ACTGCACTGA CAGCTTCACC 
CCCAACCAAG TGGCACGAAT GCACTGCTAT CTGGACCTTG TGTATCAGCA GTGGAGCCAG 
AGCCAGAAGC CCACCCCCAT CCCCATCCCC CCCATGGTCA TCGGGCAGAC TCAGAAGTCC 
CTCACTATCC ACTGGCTGCC TCCGATTAGT GGAGTTATGT ATGACAGGGT CCCGGGCAGC 
ATGTGTGGCG CCTGCACTGA AGATGGGACG TTCCGTCAGT ACGTGCACAC AGCTTCCTCC 
CAGCGCGTGT GTGACTCCTC AGGTTACTGG ACTCCGGAGG AGGCTGTGGG GCCTCCGGAC 
GTGGATCAGC CTTGTGAGCC GAGCTTGCAG GCCTGGAGTC CCGAGCTCCA CCTGCACCAC 
ACGAACATGA CGGTCCCCTG CCCTGCGGAA GGCTGCAGCC TGGAGCTGCT CTTTCAGCAC 
CCAGTCCAAG CGGACACTCT CACCCTGTGG GTCACCTACC TCTCCATGGG CTCTTCCCCG 
GTGCTCTTTG ACACAGAGAT CTTGCTGGAA CACCAGAAGT CTGTGCACTT GGGCCCCTTG 
GACACTTTCT GTGACACACC GCTCACTATC AAACTGCATG TTGATGGAAA GGTCATGGGG 
GTGAAAGTCT ACACCTTTGA TGAGCGGATG GAGATTGACG CAGCTCTCCT GACTTCTCAG 
CCCCACAGTC CCCTGTGTTC TGGCTGCAGG CCTGTGAGCT ACCAGATTCT GCGCGAGCCT 
CCGTTTGCCA GCGGCTTGCC TGCAGCGGTG ATGCATCCTC ACCGGAAGTT CACAGACATG 
GAGGTCATGC CTGGGCAGAT GTATCGGTAC CAAGTTCAAG CTGCAGCTGA AGCAGAACTG 
GGAGAAGCTT CGCCTCCTCT GAGCCACATC CATGGAGCTC CTTACTGTGG AGATGGGAAG 
GTTGCAAGGA ACCTGGGAGA AGAGTGTGAT GATGGAGACC TTTTGAATGG AGACGGCTGC 
TCAAGGGCAT GTGAGTTAGA GGAAGGTTTC AACTGTGTAG GGGAACCAAG CCTGTGCTAT 
ATACACGAGG GAGATGGAAT ATGTGAGCCT TTTGAGAAGG AAACCAGCAT TGTAGACTGT 
GGCCTCTATA CTCCCAAAGG ATACCTGGAT CAGTGGGCTA CCCAAGCTTT TGCTTCTCAT 
GAAGACACGA AGAAATGTCC TGTTTCCTTG GTAACTGGAG CACCTCATTC CATGGTTTGC 
ACATCATACC GTCCAGATTT ACCAGAGCAC CGACCCCTAA CTGGCTGGTT TCCCTGTGTC 
CCCAGTGGAA ATAGATCTCA AGATGAAGGG AGTAAGCAGC CAAAAGGTAG CCTGAAGAGA 
GAGGATGAGG TTTGGCTCAA AGTTTGTTTC AGTAGACCAG GAGTGGCCAC AGCAATTTTC 
ATTTTCTTGA CATCTGATGG CCTGGTATCT GGGAAACGTC AGCGGCCAAC AGTGACCCTT 
TACCTGACCG ATGTCAGTGG AAACAACCAC TCTCTTGGAA CCTATGAACT GTCATGCCAG 
CATAACCCGC TGGTTATCAA CATAACTCAT CACTCAAATG TCCTCTTCCA CCATGCCACA 
TCAGTGCTGC TGAATTTCTC AACCCCGTTG GTGGGCATCT CAGCGGTGGC TCTAAGGACA 
TCCTCCGACA CAAGTCCTTC AGCTCCCAAC AGCTGCATCA TCCCAGAGCA AAAGGGGCAA 
AATCATCAGG GACAAAGCTG TGTCCATCGG CCCTGTGGGG AGCAGGGCAG CTGTGTGCCA 
TTGCTGCTTG ATCACGCGGA TATGGTGAAC TGTACCTCTG GTGGCCCGGG TCTCATGAAG 
TGCTCTATCA CCTGTCAGAG GGGGTCTGCC CTTCAGGCCA GCAATGGGCA GTACCTCAGG 
CCCATGCAGA AGAAAATTCT GCTCACATGT TCCTCTGGGC ACTGGGACCG GGCTGTGAGC 
TGCCAACCCC TTGACTGTGG TGTCCCCGAC GCATCTTTGG TGAACTACGC AAATTTCTCC 
TGCTTGGAGG GAACCAACTT TCTGAAACGC TGCTCTATCT CCTGTATCCC ACCAGCCAAG 
CTTCAAGGAC TGAGCCCATG GCTGACCTGT CTTGAAGATG GGCTCTGGTC TCTCCCTGAA 
GCTTACTGCA AGCTGGAGTG TGATGCTCCT CCTGTTATCC CGAATGCCAA CCTGCTCCAG 
CCTCGCTGCC TCCAGGGGAA CCATGACGTG GGCTCTACCT GCAGATATGA ATGCAAACCG 
GGCTACTATG TGCTGGGAAG CACAGAGAAT AAAGTCAGGA ACAAGTCCCT GAAGATCCAG 
TGCCTGGAGA GTGGAATCTG GGAGCAAGGC AGCTGTGTCC CAGTGGTGTG CGAGCCCCCC 
TCCCCTGTCT TTGAAGGCAT GTATGAATGC ACCAATGGCT TTGAGCTGGA CAGCCAGTGT 
TTTATCAACT GTAACCAGGA AAGTAAAAGG CTTCCCATCC GCTGCACGAA GGAGGGACGG 
TGGAGCGAGG AGTTCAAGTT ATGTGAGAAC CTGCGAGGGG AATGCCCACC ACCCACCTCA 
GACCCCAAAT TTGTGGAGTA CAAGTGTGAA CAAGGACACG GGATAGGTGC AGTGTGTTCC 
CCATTGTGTA TCGTCCTCCC TAGTGATCCT GTAATCCTTC CTGAGAATAT CACTACTGAC 
ACTCTGGAGC ACTGGATGGA ACCCGTCAGA GTACAGAGTG TCATGTGTAC CGGACGGCGT 
CAGTGGCACC CAGACCCCCT CCTGATCCAC TGCATCCAGT CCTGCGAGGT AAGGTACTCC 
CCCTCCCTCA AACCCAGACC AGCGCTCTCT GCTCTGCTCA ACTCACAGAG ATTTGAGTCC 
TTCTGGTTCA GTATCTTGCC CTTTCTCTGC TCCGATGCCC TCTGCTCAGT CTCTTACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012021050.1
CDS	1201..6543
Translation	MMCFKILRVSLVVLAGWALSTVSSELGWTRKRSLAQRGYLNQVLSEGERCWLGAKVRRPRVAPQHHLFGVYPSLPGDYLRPYPAGDQGRAHVRCSEPDAGGMVVSSVPPDPTASARKRSEAEQPAAPWGGDSTIGQSELLGDDDTYLGDGGSKEPLGEAGTPEGTALAATVITSFPCPKESGPDTQRKGRTKTRRRRQVVKGQAGGLRGDPEFAPQHFQPWPKHPLTPRDRTGPSEGGIRNGGGDPSWEAETETVSPQGGLPVLYFSGRREPLLLRPEVLADIPREAFTVEAWVKPEGGQNSPAIIAGVFDNCSHTISDKGWALGIRSGKDVGRRDARFFFSLRTDRVKKATTVMGHSRYQPGKWTHLAATYDGQRMALYVDGTQVASSRDQSGPLNSPFMASCRSLLLGGDSSEDGHSFRGHLGTLVFWSTARPQSHLQHRPQQPLEVKDLTTLLLTASFEPLEEQWVPFRDGKYPRREVLQGFESKPEILSPLQPPLCGQTACDNVELISQYNGHWPLRGEKVIRYRVVNIYDDKGLNPTVSEEQIRRQHEVLNEAFSRYNISWQLSVHRVHNSTLRHRIVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRKDGLCHVGCNNMLNDFDEGDCCDPDVTEVRKTCFDPDSPRRAYMSVKELKEALQLNSTHFLNVYFASSVRENLAGAATWPWDKEAISHLGGVVLNPAYYGMPGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMETGDLCADTAPTPKSKLCQNPEPANDTCGFTYFPGAPFNNYMSYTDDDCTDSFTPNQVARMHCYLDLVYQQWSQSQKPTPIPIPPMVIGQTQKSLTIHWLPPISGVMYDRVPGSMCGACTEDGTFRQYVHTASSQRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLHHTNMTVPCPAEGCSLELLFQHPVQADTLTLWVTYLSMGSSPVLFDTEILLEHQKSVHLGPLDTFCDTPLTIKLHVDGKVMGVKVYTFDERMEIDAALLTSQPHSPLCSGCRPVSYQILREPPFASGLPAAVMHPHRKFTDMEVMPGQMYRYQVQAAAEAELGEASPPLSHIHGAPYCGDGKVARNLGEECDDGDLLNGDGCSRACELEEGFNCVGEPSLCYIHEGDGICEPFEKETSIVDCGLYTPKGYLDQWATQAFASHEDTKKCPVSLVTGAPHSMVCTSYRPDLPEHRPLTGWFPCVPSGNRSQDEGSKQPKGSLKREDEVWLKVCFSRPGVATAIFIFLTSDGLVSGKRQRPTVTLYLTDVSGNNHSLGTYELSCQHNPLVINITHHSNVLFHHATSVLLNFSTPLVGISAVALRTSSDTSPSAPNSCIIPEQKGQNHQGQSCVHRPCGEQGSCVPLLLDHADMVNCTSGGPGLMKCSITCQRGSALQASNGQYLRPMQKKILLTCSSGHWDRAVSCQPLDCGVPDASLVNYANFSCLEGTNFLKRCSISCIPPAKLQGLSPWLTCLEDGLWSLPEAYCKLECDAPPVIPNANLLQPRCLQGNHDVGSTCRYECKPGYYVLGSTENKVRNKSLKIQCLESGIWEQGSCVPVVCEPPSPVFEGMYECTNGFELDSQCFINCNQESKRLPIRCTKEGRWSEEFKLCENLRGECPPPTSDPKFVEYKCEQGHGIGAVCSPLCIVLPSDPVILPENITTDTLEHWMEPVRVQSVMCTGRRQWHPDPLLIHCIQSCEVRYSPSLKPRPALSALLNSQRFESFWFSILPFLCSDALCSVSYT

Target ORF information:

RefSeq Version	XM_012165660.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon pappalysin 2 (PAPPA2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012165660.2

ORF Insert Sequence:

ATGATGTGCT TCAAGATTCT GAGGGTAAGC CTGGTAGTCC TGGCTGGGTG GGCGCTCAGT 
ACTGTCAGCT CGGAGCTGGG CTGGACGCGC AAGAGATCCT TGGCCCAAAG AGGGTACCTG 
AACCAGGTGC TGTCGGAAGG AGAGCGCTGT TGGCTGGGGG CCAAGGTTCG AAGGCCCAGA 
GTTGCTCCTC AGCATCACCT CTTTGGGGTC TATCCCAGCC TGCCTGGGGA CTACCTGAGG 
CCCTACCCTG CAGGAGATCA GGGAAGGGCC CATGTAAGAT GCAGTGAGCC GGATGCCGGG 
GGAATGGTTG TGAGCTCTGT GCCCCCAGAC CCAACTGCAA GTGCAAGAAA GAGGAGTGAG 
GCTGAGCAGC CAGCTGCTCC GTGGGGAGGG GACAGTACTA TTGGGCAGTC GGAGCTACTG 
GGAGATGATG ACACTTATCT TGGTGATGGA GGATCCAAGG AGCCTCTAGG TGAGGCTGGG 
ACTCCAGAAG GCACAGCCTT GGCTGCCACT GTGATCACCA GCTTCCCATG CCCAAAGGAA 
TCCGGACCAG ACACCCAAAG GAAGGGCCGG ACCAAGACCA GGCGTCGGCG CCAGGTGGTG 
AAGGGACAGG CAGGAGGCCT GCGGGGAGAC CCTGAGTTTG CGCCCCAGCA CTTCCAGCCG 
TGGCCTAAGC ATCCCCTCAC GCCTAGGGAT CGAACCGGCC CATCAGAGGG TGGCATCCGG 
AATGGTGGAG GGGACCCCTC CTGGGAAGCA GAGACCGAGA CTGTGAGCCC CCAAGGAGGA 
CTGCCTGTCT TGTACTTCTC TGGGAGGCGG GAGCCGCTGC TGCTGCGTCC AGAAGTGCTG 
GCTGACATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAAGCCGGA GGGAGGACAG 
AACAGCCCAG CCATCATCGC AGGTGTGTTT GATAACTGCT CCCACACTAT CAGTGACAAG 
GGCTGGGCCC TGGGGATCCG CTCGGGGAAG GATGTGGGAA GGCGAGATGC TCGCTTCTTC 
TTCTCTCTTC GCACCGACCG AGTGAAGAAA GCCACCACTG TGATGGGCCA CAGTCGCTAC 
CAACCGGGCA AGTGGACACA TCTGGCAGCC ACTTACGATG GACAGCGCAT GGCCTTGTAT 
GTGGATGGCA CTCAGGTGGC TAGTAGTCGA GATCAGTCTG GTCCCTTGAA CAGCCCCTTC 
ATGGCATCTT GTCGCTCTCT CCTCCTTGGG GGGGACAGCT CGGAGGATGG GCACTCTTTC 
CGTGGACACC TGGGCACCCT GGTCTTCTGG TCGACAGCCC GCCCACAAAG CCACCTTCAG 
CACAGACCTC AGCAGCCACT GGAGGTGAAG GATTTGACCA CCCTGCTACT GACCGCCAGT 
TTTGAGCCCC TGGAAGAACA GTGGGTCCCC TTTAGAGATG GGAAGTACCC ACGGCGTGAG 
GTTCTCCAGG GCTTTGAGTC AAAGCCTGAG ATTCTGTCCC CTCTGCAGCC CCCGCTCTGT 
GGGCAGACAG CATGTGACAA TGTGGAATTG ATCTCTCAGT ACAACGGGCA CTGGCCCCTC 
CGGGGAGAGA AGGTGATCCG CTACCGGGTG GTGAACATCT ATGACGACAA GGGTCTGAAC 
CCCACAGTGA GTGAGGAGCA GATCCGTCGG CAGCACGAGG TGCTCAATGA GGCCTTCAGC 
CGCTACAACA TCAGCTGGCA GCTGAGCGTC CACCGCGTCC ACAACTCCAC CCTGCGCCAC 
CGCATTGTGC TGGTGAACTG TGAGCCCAGC AAAATCGGCA ACGATCACTG TGACCCCGAG 
TGCGAGCATC CACTCACGGG CTATGACGGG GGAGACTGCC GCCTGCAGGG CCGGTGCTAC 
TCCTGGAACC GCAAGGACGG GCTCTGCCAC GTGGGGTGCA ACAACATGCT GAATGACTTT 
GACGAGGGCG ACTGCTGTGA CCCAGACGTG ACCGAGGTGC GCAAGACCTG CTTCGACCCT 
GACTCACCCA GGAGGGCATA CATGAGTGTG AAGGAGCTGA AGGAGGCACT GCAGCTCAAC 
AGCACCCACT TCCTCAATGT CTACTTTGCC AGCTCAGTAC GAGAAAACCT TGCAGGTGCT 
GCCACCTGGC CTTGGGACAA GGAAGCCATC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
GCCTATTATG GCATGCCTGG CCACACCAAC ATCATGATCC ATGAGGTGGG GCATGTCCTG 
GGACTCTACC ATGTCTTCAA AGGGGTCAGT GAGAGAGAAT CCTGCGATGA CCCCTGCAGG 
GAGACTGTGC CGTCCATGGA GACAGGAGAC CTCTGCGCCG ACACTGCCCC TACTCCCAAG 
AGTAAGCTGT GCCAGAACCC AGAGCCTGCC AATGACACCT GTGGCTTCAC CTACTTCCCA 
GGGGCTCCAT TCAACAACTA CATGAGTTAT ACAGACGATG ACTGCACTGA CAGCTTCACC 
CCCAACCAAG TGGCACGAAT GCACTGCTAT CTGGACCTTG TGTATCAGCA GTGGAGCCAG 
AGCCAGAAGC CCACCCCCAT CCCCATCCCC CCCATGGTCA TCGGGCAGAC TCAGAAGTCC 
CTCACTATCC ACTGGCTGCC TCCGATTAGT GGAGTTATGT ATGACAGGGT CCCGGGCAGC 
ATGTGTGGCG CCTGCACTGA AGATGGGACG TTCCGTCAGT ACGTGCACAC AGCTTCCTCC 
CAGCGCGTGT GTGACTCCTC AGGTTACTGG ACTCCGGAGG AGGCTGTGGG GCCTCCGGAC 
GTGGATCAGC CTTGTGAGCC GAGCTTGCAG GCCTGGAGTC CCGAGCTCCA CCTGCACCAC 
ACGAACATGA CGGTCCCCTG CCCTGCGGAA GGCTGCAGCC TGGAGCTGCT CTTTCAGCAC 
CCAGTCCAAG CGGACACTCT CACCCTGTGG GTCACCTACC TCTCCATGGG CTCTTCCCCG 
GTGCTCTTTG ACACAGAGAT CTTGCTGGAA CACCAGAAGT CTGTGCACTT GGGCCCCTTG 
GACACTTTCT GTGACACACC GCTCACTATC AAACTGCATG TTGATGGAAA GGTCATGGGG 
GTGAAAGTCT ACACCTTTGA TGAGCGGATG GAGATTGACG CAGCTCTCCT GACTTCTCAG 
CCCCACAGTC CCCTGTGTTC TGGCTGCAGG CCTGTGAGCT ACCAGATTCT GCGCGAGCCT 
CCGTTTGCCA GCGGCTTGCC TGCAGCGGTG ATGCATCCTC ACCGGAAGTT CACAGACATG 
GAGGTCATGC CTGGGCAGAT GTATCGGTAC CAAGTTCAAG CTGCAGCTGA AGCAGAACTG 
GGAGAAGCTT CGCCTCCTCT GAGCCACATC CATGGAGCTC CTTACTGTGG AGATGGGAAG 
GTTGCAAGGA ACCTGGGAGA AGAGTGTGAT GATGGAGACC TTTTGAATGG AGACGGCTGC 
TCAAGGGCAT GTGAGTTAGA GGAAGGTTTC AACTGTGTAG GGGAACCAAG CCTGTGCTAT 
ATACACGAGG GAGATGGAAT ATGTGAGCCT TTTGAGAAGG AAACCAGCAT TGTAGACTGT 
GGCCTCTATA CTCCCAAAGG ATACCTGGAT CAGTGGGCTA CCCAAGCTTT TGCTTCTCAT 
GAAGACACGA AGAAATGTCC TGTTTCCTTG GTAACTGGAG CACCTCATTC CATGGTTTGC 
ACATCATACC GTCCAGATTT ACCAGAGCAC CGACCCCTAA CTGGCTGGTT TCCCTGTGTC 
CCCAGTGGAA ATAGATCTCA AGATGAAGGG AGTAAGCAGC CAAAAGGTAG CCTGAAGAGA 
GAGGATGAGG TTTGGCTCAA AGTTTGTTTC AGTAGACCAG GAGTGGCCAC AGCAATTTTC 
ATTTTCTTGA CATCTGATGG CCTGGTATCT GGGAAACGTC AGCGGCCAAC AGTGACCCTT 
TACCTGACCG ATGTCAGTGG AAACAACCAC TCTCTTGGAA CCTATGAACT GTCATGCCAG 
CATAACCCGC TGGTTATCAA CATAACTCAT CACTCAAATG TCCTCTTCCA CCATGCCACA 
TCAGTGCTGC TGAATTTCTC AACCCCGTTG GTGGGCATCT CAGCGGTGGC TCTAAGGACA 
TCCTCCGACA CAAGTCCTTC AGCTCCCAAC AGCTGCATCA TCCCAGAGCA AAAGGGGCAA 
AATCATCAGG GACAAAGCTG TGTCCATCGG CCCTGTGGGG AGCAGGGCAG CTGTGTGCCA 
TTGCTGCTTG ATCACGCGGA TATGGTGAAC TGTACCTCTG GTGGCCCGGG TCTCATGAAG 
TGCTCTATCA CCTGTCAGAG GGGGTCTGCC CTTCAGGCCA GCAATGGGCA GTACCTCAGG 
CCCATGCAGA AGAAAATTCT GCTCACATGT TCCTCTGGGC ACTGGGACCG GGCTGTGAGC 
TGCCAACCCC TTGACTGTGG TGTCCCCGAC GCATCTTTGG TGAACTACGC AAATTTCTCC 
TGCTTGGAGG GAACCAACTT TCTGAAACGC TGCTCTATCT CCTGTATCCC ACCAGCCAAG 
CTTCAAGGAC TGAGCCCATG GCTGACCTGT CTTGAAGATG GGCTCTGGTC TCTCCCTGAA 
GCTTACTGCA AGCTGGAGTG TGATGCTCCT CCTGTTATCC CGAATGCCAA CCTGCTCCAG 
CCTCGCTGCC TCCAGGGGAA CCATGACGTG GGCTCTACCT GCAGATATGA ATGCAAACCG 
GGCTACTATG TGCTGGGAAG CACAGAGAAT AAAGTCAGGA ACAAGTCCCT GAAGATCCAG 
TGCCTGGAGA GTGGAATCTG GGAGCAAGGC AGCTGTGTCC CAGTGGTGTG CGAGCCCCCC 
TCCCCTGTCT TTGAAGGCAT GTATGAATGC ACCAATGGCT TTGAGCTGGA CAGCCAGTGT 
TTTATCAACT GTAACCAGGA AAGTAAAAGG CTTCCCATCC GCTGCACGAA GGAGGGACGG 
TGGAGCGAGG AGTTCAAGTT ATGTGAGAAC CTGCGAGGGG AATGCCCACC ACCCACCTCA 
GACCCCAAAT TTGTGGAGTA CAAGTGTGAA CAAGGACACG GGATAGGTGC AGTGTGTTCC 
CCATTGTGTA TCGTCCTCCC TAGTGATCCT GTAATCCTTC CTGAGAATAT CACTACTGAC 
ACTCTGGAGC ACTGGATGGA ACCCGTCAGA GTACAGAGTG TCATGTGTAC CGGACGGCGT 
CAGTGGCACC CAGACCCCCT CCTGATCCAC TGCATCCAGT CCTGCGAGGT AAGGTACTCC 
CCCTCCCTCA AACCCAGACC AGCGCTCTCT GCTCTGCTCA ACTCACAGAG ATTTGAGTCC 
TTCTGGTTCA GTATCTTGCC CTTTCTCTGC TCCGATGCCC TCTGCTCAGT CTCTTACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( XM_004013823.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004013872.1 Ovis aries pappalysin 2 (PAPPA2), transcript variant X1, mRNA.

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CloneID	OSm10754
Clone ID Related Accession (Same CDS sequence)	XM_004013823.3 , XM_012187595.2
Accession Version	XM_004013823.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	pappalysin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639021.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_004013823.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCT TCAAGATTCT GAGGGTAAGC CTGGTAGTCC TGGCTGGGTG GGCGCTCAGT 
ACTGTCAGCT CAGAGCTGGG CTGGACGCGC AAGAGATCCT TGGCCCAAAG AGGGTACCTG 
AACCAGGTGC TGTCGGAAGG AGAGCGCTGT TGGCTGGGGG CCAAGGTTCG AAGGCCCAGA 
GTTGCTCCTC AGCATCACCT CTTTGGGGTC TATCCCAGCC TGCCTGGGGA CTACCTGAGG 
CCCTACCCTG CAGGAGATCA GGGAAGGGCC CATGTAAGAT GCAGTGAGCC GGATGCCGGG 
GGAATGGTTG TGAGCTCTGT GCCCCCAGAC CCAACTGCAA GTGCAAGAAA GAGGAGTGAG 
GCTGAGCAGC CAGCTGCTCC GTGGGGAGGG GACAGTACTA TTGGGCAGTC GGAGCTACTG 
GGAGATGATG ACACTTATCT TGGTGATGGA GGATCCAAGG AGCCTCTAGG TGAGGCTGGG 
ACTCCAGAAG GCACAGCCTT GGCTGCCACT GTGATCACCA GCTTCCCATG CCCAAAGGAA 
TCCGGACCAG ACACCCAAAG GAAGGGCCGG ACCAAGACCA GGCGTCGGCG CCAGGTGGTG 
AAGGGACAGG CAGGAGGCCT GCGGGGAGAC CCTGAGTTTG CGCCCCAGCA CTTCCAGCCG 
TGGCCTAAGC ATCCCCTCAC GCCTAGGGAT CGAACCGGCC CATCAGAGGG TGGCATCCGG 
AATGGTGGAG GGGACCCCTC CTGGGAAGCA GAGACCGAGA CTGTGAGCCC CCAAGGAGGA 
CTGCCTGTCT TGTACTTCTC TGGGAGGCGG GAGCCGCTGC TGCTGCGTCC AGAAGTGCTG 
GCTGACATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAAGCCGGA GGGAGGACAG 
AACAGCCCAG CCATCATCGC AGGTGTGTTT GATAACTGCT CCCACACTAT CAGTGACAAG 
GGCTGGGCCC TGGGGATCCG CTCGGGGAAG GATGTGGGAA GGCGAGATGC TCGCTTCTTC 
TTCTCTCTTC GCACCGACCG AGTGAAGAAA GCCACCACTG TGATGGGCCA CAGTCGCTAC 
CAACCGGGCA AGTGGACACA TCTGGCAGCC ACTTATGATG GACAGCGCAT GGCCTTGTAT 
GTGGATGGCA CTCAGGTGGC TAGTAGTCGA GATCAGTCTG GTCCCTTGAA CAGCCCCTTC 
ATGGCATCTT GTCGCTCTCT CCTCCTTGGG GGGGACAGCT CGGAGGACGG GCACTCTTTC 
CGTGGACACC TGGGCACCCT GGTCTTCTGG TCGACAGCCC GCCCACAAAG CCACCTTCAG 
CACAGACCTC AGCAGCCACT GGAGGTGAAG GATTTGACCA CCCTGCTACT GACCGCCAGT 
TTTGAGCCCC TGGAAGAACA GTGGGTCCCC TTTAGAGACG GGAAGTACCC ACGGCGTGAG 
GTTCTCCAGG GCTTTGAGTC AAAGCCTGAG ATTCTGTCCC CTCTGCAGCC CCCGCTCTGT 
GGGCAGACAG CATGTGACAA TGTGGAATTG ATCTCTCAGT ACAATGGGCA CTGGCCCCTC 
CGGGGAGAGA AGGTGATCCG CTACCGGGTG GTGAACATCT ATGACGACAA GGGTCTGAAC 
CCCACGGTGA GTGAGGAGCA GATCCGTCGG CAGCACGAGG TGCTCAATGA GGCCTTCAGC 
CGCTACAACA TCAGCTGGCA GCTGAGCGTC CACCGCGTCC ACAACTCCAC CCTGCGCCAC 
CGCATTGTGC TGGTGAACTG TGAGCCCAGC AAAATCGGCA ACGATCACTG TGACCCCGAG 
TGCGAGCATC CACTCACGGG CTACGACGGG GGAGACTGCC GCCTGCAGGG CCGGTGCTAC 
TCCTGGAACC GCAAGGACGG GCTCTGCCAC GTGGGGTGCA ACAACATGCT GAATGACTTT 
GACGAGGGCG ACTGCTGTGA CCCAGACGTG ACCGAGGTGC GCAAGACCTG CTTCGACCCT 
GACTCACCCA GGAGGGCATA CATGAGTGTG AAGGAGCTGA AGGAGGCACT GCAGCTCAAC 
AGCACCCACT TCCTCAATGT CTACTTTGCC AGCTCAGTAC GAGAAAACCT TGCAGGTGCT 
GCCACCTGGC CTTGGGACAA GGAAGCCATC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
GCCTATTATG GCATGCCTGG CCACACCAAC ATCATGATCC ATGAGGTGGG GCATGTCCTG 
GGACTCTACC ATGTCTTCAA AGGGGTCAGT GAGAGAGAAT CCTGCGATGA CCCCTGCAGG 
GAGACTGTGC CGTCCATGGA GACAGGAGAC CTCTGCGCCG ACACTGCCCC TACTCCCAAG 
AGTAAGCTGT GCCAGAACCC AGAGCCTGCC AATGACACCT GTGGCTTCAC CTACTTCCCA 
GGGGCTCCAT TCAACAACTA CATGAGTTAT ACAGACGATG ACTGCACTGA CAGCTTCACC 
CCCAACCAAG TGGCAAGAAT GCACTGCTAT CTGGACCTTG TGTATCAGCA GTGGAGCCAG 
AGCCAGAAGC CCACCCCCAT CCCCATCCCC CCCATGGTCA TCGGGCAGAC TCAGAAGTCC 
CTCACTATCC ACTGGCTGCC TCCGATTAGT GGAGTTATGT ATGACAGGGT CCCGGGCAGC 
ATGTGTGGCG CCTGCACTGA AGATGGGACG TTCCGTCAGT ACGTGCACAC AGCTTCCTCC 
CAGCGCGTGT GTGACTCCTC AGGTTACTGG ACTCCGGAGG AGGCTGTGGG GCCTCCGGAC 
GTGGATCAGC CTTGTGAGCC GAGCTTGCAG GCCTGGAGTC CCGAGCTCCA CCTGCACCAC 
ACGAACATGA CGGTCCCCTG CCCTGCGGAA GGCTGCAGCC TGGAGCTGCT CTTTCAGCAC 
CCAGTCCAAG CGGACACTCT CACCCTGTGG GTCACCTACC TCTCCATGGG CTCTTCCCCG 
GTGCTCTTTG ACACAGAGAT CTTGCTGGAA CACCAGAAGT CTGTGCACCT GGGCCCCTTG 
GACACTTTCT GTGACACACC GCTCACTATC AAACTGCATG TTGATGGAAA GGTCATGGGG 
GTGAAAGTCT ACACCTTTGA TGAGCGGATG GAGATTGACG CAGCTCTCCT GACTTCTCAG 
CCCCACAGTC CCCTGTGTTC TGGCTGCAGG CCTGTGAGCT ACCAGATTCT CCGCGAGCCT 
CCGTTTGCCA GCGGCTTGCC TGCAGCGGTG ATGCATCCTC ACCGGAAGTT CACAGACATG 
GAGGTCATGC CTGGGCAGAT GTATCGGTAC CAAGTTCAAG CTGCAGCTGA AGCAGAACTG 
GGAGAAGCTT CGCCTCCTCT GAGCCACATC CATGGAGCTC CTTACTGTGG AGATGGGAAG 
GTTGCAAGGA ACCTGGGAGA AGAGTGTGAT GATGGAGACC TTTTGAATGG AGACGGCTGC 
TCAAGGGCAT GTGAGTTAGA GGAAGGTTTC AACTGTGTAG GGGAACCAAG CCTGTGCTAT 
ATACACGAAG GAGATGGAAT ATGTGAGCCT TTTGAGAAGG AAACCAGCAT TGTAGACTGT 
GGCCTCTATA CTCCCAAAGG ATACCTGGAT CAGTGGGCTA CCCAAGCTTT TGCTTCTCAT 
GAAGACACGA AGAAATGTCC TGTTTCCTTG GTAACTGGAG CACCTCATTC CATGGTTTGC 
ACATCATACC GTCCAGATTT ACCAGAGCAC CCACCCCTAA CTGGCTGGTT TCCCTGTGTC 
CCCAGTGGAA ATAGATCTCA AGATGAAGGG AGTAAGCAGC CAAAAGGTAG CCTGAAGAGA 
GAGGATGAGG TTTGGCTCAA AGTTTGTTTC AGTAGACCAG GAGTGGCCAC AGCAATTTTC 
ATTTTCTTGA CATCTGATGG CCTGGTATCT GGGAAACGTC AGCGGCCAAC AGTGACCCTT 
TACCTGACCG ATGTCAGTGG AAACAACCAC TCTCTTGGAA CCTATGAACT GTCATGCCAG 
CATAACCCGC TGGTTATCAA CATAACTCAT CACTCAAATG TCCTCTTCCA CCATGCCACA 
TCAGTGCTGC TGAATTTCTC AACCCCGTTG GTGGGCATCT CAGCGGTGGC TCTAAGGACA 
TCCTCCGACA CAAGTCCTTC AGCTCCCAAC AGCTGCATCA TCCCAGAGCA AAAGGGGCAA 
AATCATCAGG GACAAAGCTG TGTCCATCGG CCCTGTGGGG AGCAGGGCAG CTGTGTGCCA 
TTGCTGCTTG ATCACGCGGA TATGGTGAAC TGTACCTCTG GTGGCCCGGG TCTCATGAAG 
TGCTCTATCA CCTGTCAGAG GGGGTCTGCC CTTCAGGCCA GCAATGGGCA GTACCTCAGG 
CCCCTGCAGA AGAAAATTCT GCTCACATGT TCCTCTGGGC ACTGGGACGG GGCTGTGAGC 
TGCCAACCCC TTGACTGTGG TGTCCCCGAC GCATCTTTGG TGAACTACGC AAATTTCTCC 
TGCTTGGAGG GAACCAACTT TCTGAAACGC TGCTCTATCT CCTGTATCCC ACCAGCCAAG 
CTTCAAGGAC TGAGCCCATG GCTGACCTGT CTTGAAGATG GGCTCTGGTC TCTCCCTGAA 
GCTTACTGCA AGCTGGAGTG TGATGCTCCT CCTGTTATCC CGAATGCCAA CCTGCTCCAG 
CCTCGCTGCC TCCAGGGGAA CCATGACGTG GGCTCTACCT GCAGATATGA ATGCAAACCG 
GGCTACTATG TGCTGGGAAG CACGGAGAAT AAAGTCAGGA ACAAGTCCCT GAAGATCCAG 
TGCCTGGAGA GTGGAATCTG GGAGCAAGGC AGCTGTGTCC CAGTGGTGTG CGAGCCCCCC 
TCCCCTGTCT TTGAAGGCAT GTATGAATGC ACCAATGGCT TTGAGCTGGA CAGCCAGTGT 
TTTATCAACT GTAACCAGGA AAGTAAAAGG CTTCCCATCC GCTGCACGAA GGAGGGACGG 
TGGAGCGAGG AGTTCAAGTT ATGTGAGAAC CTGCGAGGGG AATGCCCACC ACCCACCTCA 
GACCCCAAAT TTGTGGAGTA CAAGTGTGAA CAAGGACATG GGATAGGTGC AGTGTGTTCC 
CCATTGTGTA TCGTCCTCCC TAGTGATCCT GTAATCCTTC CTGAGAATAT CACTACTGAC 
ACTCTGGAGC ACTGGATGGA ACCCGTCAGA GTACAGAGTG TCATGTGTAC CGGACGGCGT 
CAGTGGCACC CAGACCCCCT CCTGATCCAC TGCATCCAGT CCTGCGAGCC TTTCCAAGGG 
GACGGTTGGT GTGACACCAT CAATAACCGG GCCTATTGCA ACTATGATGG GGGAGACTGC 
TGCCCTTCTA CACTCTCCTC CAAGAAGGTC ATTCCATTTG CTGCTGACTG TGATCAGGAT 
GAATGCACCT GCCGAGACCC CAGGGCTGAA GAAAACCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004013872.1
CDS	1757..7138
Translation	MMCFKILRVSLVVLAGWALSTVSSELGWTRKRSLAQRGYLNQVLSEGERCWLGAKVRRPRVAPQHHLFGVYPSLPGDYLRPYPAGDQGRAHVRCSEPDAGGMVVSSVPPDPTASARKRSEAEQPAAPWGGDSTIGQSELLGDDDTYLGDGGSKEPLGEAGTPEGTALAATVITSFPCPKESGPDTQRKGRTKTRRRRQVVKGQAGGLRGDPEFAPQHFQPWPKHPLTPRDRTGPSEGGIRNGGGDPSWEAETETVSPQGGLPVLYFSGRREPLLLRPEVLADIPREAFTVEAWVKPEGGQNSPAIIAGVFDNCSHTISDKGWALGIRSGKDVGRRDARFFFSLRTDRVKKATTVMGHSRYQPGKWTHLAATYDGQRMALYVDGTQVASSRDQSGPLNSPFMASCRSLLLGGDSSEDGHSFRGHLGTLVFWSTARPQSHLQHRPQQPLEVKDLTTLLLTASFEPLEEQWVPFRDGKYPRREVLQGFESKPEILSPLQPPLCGQTACDNVELISQYNGHWPLRGEKVIRYRVVNIYDDKGLNPTVSEEQIRRQHEVLNEAFSRYNISWQLSVHRVHNSTLRHRIVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRKDGLCHVGCNNMLNDFDEGDCCDPDVTEVRKTCFDPDSPRRAYMSVKELKEALQLNSTHFLNVYFASSVRENLAGAATWPWDKEAISHLGGVVLNPAYYGMPGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMETGDLCADTAPTPKSKLCQNPEPANDTCGFTYFPGAPFNNYMSYTDDDCTDSFTPNQVARMHCYLDLVYQQWSQSQKPTPIPIPPMVIGQTQKSLTIHWLPPISGVMYDRVPGSMCGACTEDGTFRQYVHTASSQRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLHHTNMTVPCPAEGCSLELLFQHPVQADTLTLWVTYLSMGSSPVLFDTEILLEHQKSVHLGPLDTFCDTPLTIKLHVDGKVMGVKVYTFDERMEIDAALLTSQPHSPLCSGCRPVSYQILREPPFASGLPAAVMHPHRKFTDMEVMPGQMYRYQVQAAAEAELGEASPPLSHIHGAPYCGDGKVARNLGEECDDGDLLNGDGCSRACELEEGFNCVGEPSLCYIHEGDGICEPFEKETSIVDCGLYTPKGYLDQWATQAFASHEDTKKCPVSLVTGAPHSMVCTSYRPDLPEHPPLTGWFPCVPSGNRSQDEGSKQPKGSLKREDEVWLKVCFSRPGVATAIFIFLTSDGLVSGKRQRPTVTLYLTDVSGNNHSLGTYELSCQHNPLVINITHHSNVLFHHATSVLLNFSTPLVGISAVALRTSSDTSPSAPNSCIIPEQKGQNHQGQSCVHRPCGEQGSCVPLLLDHADMVNCTSGGPGLMKCSITCQRGSALQASNGQYLRPLQKKILLTCSSGHWDGAVSCQPLDCGVPDASLVNYANFSCLEGTNFLKRCSISCIPPAKLQGLSPWLTCLEDGLWSLPEAYCKLECDAPPVIPNANLLQPRCLQGNHDVGSTCRYECKPGYYVLGSTENKVRNKSLKIQCLESGIWEQGSCVPVVCEPPSPVFEGMYECTNGFELDSQCFINCNQESKRLPIRCTKEGRWSEEFKLCENLRGECPPPTSDPKFVEYKCEQGHGIGAVCSPLCIVLPSDPVILPENITTDTLEHWMEPVRVQSVMCTGRRQWHPDPLLIHCIQSCEPFQGDGWCDTINNRAYCNYDGGDCCPSTLSSKKVIPFAADCDQDECTCRDPRAEENQ

Target ORF information:

RefSeq Version	XM_004013823.3
Organism	Ovis aries(sheep)
Definition	Ovis aries pappalysin 2 (PAPPA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004013823.3

ORF Insert Sequence:

ATGATGTGCT TCAAGATTCT GAGGGTAAGC CTGGTAGTCC TGGCTGGGTG GGCGCTCAGT 
ACTGTCAGCT CAGAGCTGGG CTGGACGCGC AAGAGATCCT TGGCCCAAAG AGGGTACCTG 
AACCAGGTGC TGTCGGAAGG AGAGCGCTGT TGGCTGGGGG CCAAGGTTCG AAGGCCCAGA 
GTTGCTCCTC AGCATCACCT CTTTGGGGTC TATCCCAGCC TGCCTGGGGA CTACCTGAGG 
CCCTACCCTG CAGGAGATCA GGGAAGGGCC CATGTAAGAT GCAGTGAGCC GGATGCCGGG 
GGAATGGTTG TGAGCTCTGT GCCCCCAGAC CCAACTGCAA GTGCAAGAAA GAGGAGTGAG 
GCTGAGCAGC CAGCTGCTCC GTGGGGAGGG GACAGTACTA TTGGGCAGTC GGAGCTACTG 
GGAGATGATG ACACTTATCT TGGTGATGGA GGATCCAAGG AGCCTCTAGG TGAGGCTGGG 
ACTCCAGAAG GCACAGCCTT GGCTGCCACT GTGATCACCA GCTTCCCATG CCCAAAGGAA 
TCCGGACCAG ACACCCAAAG GAAGGGCCGG ACCAAGACCA GGCGTCGGCG CCAGGTGGTG 
AAGGGACAGG CAGGAGGCCT GCGGGGAGAC CCTGAGTTTG CGCCCCAGCA CTTCCAGCCG 
TGGCCTAAGC ATCCCCTCAC GCCTAGGGAT CGAACCGGCC CATCAGAGGG TGGCATCCGG 
AATGGTGGAG GGGACCCCTC CTGGGAAGCA GAGACCGAGA CTGTGAGCCC CCAAGGAGGA 
CTGCCTGTCT TGTACTTCTC TGGGAGGCGG GAGCCGCTGC TGCTGCGTCC AGAAGTGCTG 
GCTGACATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAAGCCGGA GGGAGGACAG 
AACAGCCCAG CCATCATCGC AGGTGTGTTT GATAACTGCT CCCACACTAT CAGTGACAAG 
GGCTGGGCCC TGGGGATCCG CTCGGGGAAG GATGTGGGAA GGCGAGATGC TCGCTTCTTC 
TTCTCTCTTC GCACCGACCG AGTGAAGAAA GCCACCACTG TGATGGGCCA CAGTCGCTAC 
CAACCGGGCA AGTGGACACA TCTGGCAGCC ACTTATGATG GACAGCGCAT GGCCTTGTAT 
GTGGATGGCA CTCAGGTGGC TAGTAGTCGA GATCAGTCTG GTCCCTTGAA CAGCCCCTTC 
ATGGCATCTT GTCGCTCTCT CCTCCTTGGG GGGGACAGCT CGGAGGACGG GCACTCTTTC 
CGTGGACACC TGGGCACCCT GGTCTTCTGG TCGACAGCCC GCCCACAAAG CCACCTTCAG 
CACAGACCTC AGCAGCCACT GGAGGTGAAG GATTTGACCA CCCTGCTACT GACCGCCAGT 
TTTGAGCCCC TGGAAGAACA GTGGGTCCCC TTTAGAGACG GGAAGTACCC ACGGCGTGAG 
GTTCTCCAGG GCTTTGAGTC AAAGCCTGAG ATTCTGTCCC CTCTGCAGCC CCCGCTCTGT 
GGGCAGACAG CATGTGACAA TGTGGAATTG ATCTCTCAGT ACAATGGGCA CTGGCCCCTC 
CGGGGAGAGA AGGTGATCCG CTACCGGGTG GTGAACATCT ATGACGACAA GGGTCTGAAC 
CCCACGGTGA GTGAGGAGCA GATCCGTCGG CAGCACGAGG TGCTCAATGA GGCCTTCAGC 
CGCTACAACA TCAGCTGGCA GCTGAGCGTC CACCGCGTCC ACAACTCCAC CCTGCGCCAC 
CGCATTGTGC TGGTGAACTG TGAGCCCAGC AAAATCGGCA ACGATCACTG TGACCCCGAG 
TGCGAGCATC CACTCACGGG CTACGACGGG GGAGACTGCC GCCTGCAGGG CCGGTGCTAC 
TCCTGGAACC GCAAGGACGG GCTCTGCCAC GTGGGGTGCA ACAACATGCT GAATGACTTT 
GACGAGGGCG ACTGCTGTGA CCCAGACGTG ACCGAGGTGC GCAAGACCTG CTTCGACCCT 
GACTCACCCA GGAGGGCATA CATGAGTGTG AAGGAGCTGA AGGAGGCACT GCAGCTCAAC 
AGCACCCACT TCCTCAATGT CTACTTTGCC AGCTCAGTAC GAGAAAACCT TGCAGGTGCT 
GCCACCTGGC CTTGGGACAA GGAAGCCATC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
GCCTATTATG GCATGCCTGG CCACACCAAC ATCATGATCC ATGAGGTGGG GCATGTCCTG 
GGACTCTACC ATGTCTTCAA AGGGGTCAGT GAGAGAGAAT CCTGCGATGA CCCCTGCAGG 
GAGACTGTGC CGTCCATGGA GACAGGAGAC CTCTGCGCCG ACACTGCCCC TACTCCCAAG 
AGTAAGCTGT GCCAGAACCC AGAGCCTGCC AATGACACCT GTGGCTTCAC CTACTTCCCA 
GGGGCTCCAT TCAACAACTA CATGAGTTAT ACAGACGATG ACTGCACTGA CAGCTTCACC 
CCCAACCAAG TGGCAAGAAT GCACTGCTAT CTGGACCTTG TGTATCAGCA GTGGAGCCAG 
AGCCAGAAGC CCACCCCCAT CCCCATCCCC CCCATGGTCA TCGGGCAGAC TCAGAAGTCC 
CTCACTATCC ACTGGCTGCC TCCGATTAGT GGAGTTATGT ATGACAGGGT CCCGGGCAGC 
ATGTGTGGCG CCTGCACTGA AGATGGGACG TTCCGTCAGT ACGTGCACAC AGCTTCCTCC 
CAGCGCGTGT GTGACTCCTC AGGTTACTGG ACTCCGGAGG AGGCTGTGGG GCCTCCGGAC 
GTGGATCAGC CTTGTGAGCC GAGCTTGCAG GCCTGGAGTC CCGAGCTCCA CCTGCACCAC 
ACGAACATGA CGGTCCCCTG CCCTGCGGAA GGCTGCAGCC TGGAGCTGCT CTTTCAGCAC 
CCAGTCCAAG CGGACACTCT CACCCTGTGG GTCACCTACC TCTCCATGGG CTCTTCCCCG 
GTGCTCTTTG ACACAGAGAT CTTGCTGGAA CACCAGAAGT CTGTGCACCT GGGCCCCTTG 
GACACTTTCT GTGACACACC GCTCACTATC AAACTGCATG TTGATGGAAA GGTCATGGGG 
GTGAAAGTCT ACACCTTTGA TGAGCGGATG GAGATTGACG CAGCTCTCCT GACTTCTCAG 
CCCCACAGTC CCCTGTGTTC TGGCTGCAGG CCTGTGAGCT ACCAGATTCT CCGCGAGCCT 
CCGTTTGCCA GCGGCTTGCC TGCAGCGGTG ATGCATCCTC ACCGGAAGTT CACAGACATG 
GAGGTCATGC CTGGGCAGAT GTATCGGTAC CAAGTTCAAG CTGCAGCTGA AGCAGAACTG 
GGAGAAGCTT CGCCTCCTCT GAGCCACATC CATGGAGCTC CTTACTGTGG AGATGGGAAG 
GTTGCAAGGA ACCTGGGAGA AGAGTGTGAT GATGGAGACC TTTTGAATGG AGACGGCTGC 
TCAAGGGCAT GTGAGTTAGA GGAAGGTTTC AACTGTGTAG GGGAACCAAG CCTGTGCTAT 
ATACACGAAG GAGATGGAAT ATGTGAGCCT TTTGAGAAGG AAACCAGCAT TGTAGACTGT 
GGCCTCTATA CTCCCAAAGG ATACCTGGAT CAGTGGGCTA CCCAAGCTTT TGCTTCTCAT 
GAAGACACGA AGAAATGTCC TGTTTCCTTG GTAACTGGAG CACCTCATTC CATGGTTTGC 
ACATCATACC GTCCAGATTT ACCAGAGCAC CCACCCCTAA CTGGCTGGTT TCCCTGTGTC 
CCCAGTGGAA ATAGATCTCA AGATGAAGGG AGTAAGCAGC CAAAAGGTAG CCTGAAGAGA 
GAGGATGAGG TTTGGCTCAA AGTTTGTTTC AGTAGACCAG GAGTGGCCAC AGCAATTTTC 
ATTTTCTTGA CATCTGATGG CCTGGTATCT GGGAAACGTC AGCGGCCAAC AGTGACCCTT 
TACCTGACCG ATGTCAGTGG AAACAACCAC TCTCTTGGAA CCTATGAACT GTCATGCCAG 
CATAACCCGC TGGTTATCAA CATAACTCAT CACTCAAATG TCCTCTTCCA CCATGCCACA 
TCAGTGCTGC TGAATTTCTC AACCCCGTTG GTGGGCATCT CAGCGGTGGC TCTAAGGACA 
TCCTCCGACA CAAGTCCTTC AGCTCCCAAC AGCTGCATCA TCCCAGAGCA AAAGGGGCAA 
AATCATCAGG GACAAAGCTG TGTCCATCGG CCCTGTGGGG AGCAGGGCAG CTGTGTGCCA 
TTGCTGCTTG ATCACGCGGA TATGGTGAAC TGTACCTCTG GTGGCCCGGG TCTCATGAAG 
TGCTCTATCA CCTGTCAGAG GGGGTCTGCC CTTCAGGCCA GCAATGGGCA GTACCTCAGG 
CCCCTGCAGA AGAAAATTCT GCTCACATGT TCCTCTGGGC ACTGGGACGG GGCTGTGAGC 
TGCCAACCCC TTGACTGTGG TGTCCCCGAC GCATCTTTGG TGAACTACGC AAATTTCTCC 
TGCTTGGAGG GAACCAACTT TCTGAAACGC TGCTCTATCT CCTGTATCCC ACCAGCCAAG 
CTTCAAGGAC TGAGCCCATG GCTGACCTGT CTTGAAGATG GGCTCTGGTC TCTCCCTGAA 
GCTTACTGCA AGCTGGAGTG TGATGCTCCT CCTGTTATCC CGAATGCCAA CCTGCTCCAG 
CCTCGCTGCC TCCAGGGGAA CCATGACGTG GGCTCTACCT GCAGATATGA ATGCAAACCG 
GGCTACTATG TGCTGGGAAG CACGGAGAAT AAAGTCAGGA ACAAGTCCCT GAAGATCCAG 
TGCCTGGAGA GTGGAATCTG GGAGCAAGGC AGCTGTGTCC CAGTGGTGTG CGAGCCCCCC 
TCCCCTGTCT TTGAAGGCAT GTATGAATGC ACCAATGGCT TTGAGCTGGA CAGCCAGTGT 
TTTATCAACT GTAACCAGGA AAGTAAAAGG CTTCCCATCC GCTGCACGAA GGAGGGACGG 
TGGAGCGAGG AGTTCAAGTT ATGTGAGAAC CTGCGAGGGG AATGCCCACC ACCCACCTCA 
GACCCCAAAT TTGTGGAGTA CAAGTGTGAA CAAGGACATG GGATAGGTGC AGTGTGTTCC 
CCATTGTGTA TCGTCCTCCC TAGTGATCCT GTAATCCTTC CTGAGAATAT CACTACTGAC 
ACTCTGGAGC ACTGGATGGA ACCCGTCAGA GTACAGAGTG TCATGTGTAC CGGACGGCGT 
CAGTGGCACC CAGACCCCCT CCTGATCCAC TGCATCCAGT CCTGCGAGCC TTTCCAAGGG 
GACGGTTGGT GTGACACCAT CAATAACCGG GCCTATTGCA ACTATGATGG GGGAGACTGC 
TGCCCTTCTA CACTCTCCTC CAAGAAGGTC ATTCCATTTG CTGCTGACTG TGATCAGGAT 
GAATGCACCT GCCGAGACCC CAGGGCTGAA GAAAACCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( XM_012187595.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012042985.1 Ovis aries pappalysin 2 (PAPPA2), transcript variant X2, mRNA.

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CloneID	OSm10754
Clone ID Related Accession (Same CDS sequence)	XM_004013823.3 , XM_012187595.2
Accession Version	XM_012187595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450195200000
Organism	Ovis aries(sheep)
Product	pappalysin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639021.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012187595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCT TCAAGATTCT GAGGGTAAGC CTGGTAGTCC TGGCTGGGTG GGCGCTCAGT 
ACTGTCAGCT CAGAGCTGGG CTGGACGCGC AAGAGATCCT TGGCCCAAAG AGGGTACCTG 
AACCAGGTGC TGTCGGAAGG AGAGCGCTGT TGGCTGGGGG CCAAGGTTCG AAGGCCCAGA 
GTTGCTCCTC AGCATCACCT CTTTGGGGTC TATCCCAGCC TGCCTGGGGA CTACCTGAGG 
CCCTACCCTG CAGGAGATCA GGGAAGGGCC CATGTAAGAT GCAGTGAGCC GGATGCCGGG 
GGAATGGTTG TGAGCTCTGT GCCCCCAGAC CCAACTGCAA GTGCAAGAAA GAGGAGTGAG 
GCTGAGCAGC CAGCTGCTCC GTGGGGAGGG GACAGTACTA TTGGGCAGTC GGAGCTACTG 
GGAGATGATG ACACTTATCT TGGTGATGGA GGATCCAAGG AGCCTCTAGG TGAGGCTGGG 
ACTCCAGAAG GCACAGCCTT GGCTGCCACT GTGATCACCA GCTTCCCATG CCCAAAGGAA 
TCCGGACCAG ACACCCAAAG GAAGGGCCGG ACCAAGACCA GGCGTCGGCG CCAGGTGGTG 
AAGGGACAGG CAGGAGGCCT GCGGGGAGAC CCTGAGTTTG CGCCCCAGCA CTTCCAGCCG 
TGGCCTAAGC ATCCCCTCAC GCCTAGGGAT CGAACCGGCC CATCAGAGGG TGGCATCCGG 
AATGGTGGAG GGGACCCCTC CTGGGAAGCA GAGACCGAGA CTGTGAGCCC CCAAGGAGGA 
CTGCCTGTCT TGTACTTCTC TGGGAGGCGG GAGCCGCTGC TGCTGCGTCC AGAAGTGCTG 
GCTGACATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAAGCCGGA GGGAGGACAG 
AACAGCCCAG CCATCATCGC AGGTGTGTTT GATAACTGCT CCCACACTAT CAGTGACAAG 
GGCTGGGCCC TGGGGATCCG CTCGGGGAAG GATGTGGGAA GGCGAGATGC TCGCTTCTTC 
TTCTCTCTTC GCACCGACCG AGTGAAGAAA GCCACCACTG TGATGGGCCA CAGTCGCTAC 
CAACCGGGCA AGTGGACACA TCTGGCAGCC ACTTATGATG GACAGCGCAT GGCCTTGTAT 
GTGGATGGCA CTCAGGTGGC TAGTAGTCGA GATCAGTCTG GTCCCTTGAA CAGCCCCTTC 
ATGGCATCTT GTCGCTCTCT CCTCCTTGGG GGGGACAGCT CGGAGGACGG GCACTCTTTC 
CGTGGACACC TGGGCACCCT GGTCTTCTGG TCGACAGCCC GCCCACAAAG CCACCTTCAG 
CACAGACCTC AGCAGCCACT GGAGGTGAAG GATTTGACCA CCCTGCTACT GACCGCCAGT 
TTTGAGCCCC TGGAAGAACA GTGGGTCCCC TTTAGAGACG GGAAGTACCC ACGGCGTGAG 
GTTCTCCAGG GCTTTGAGTC AAAGCCTGAG ATTCTGTCCC CTCTGCAGCC CCCGCTCTGT 
GGGCAGACAG CATGTGACAA TGTGGAATTG ATCTCTCAGT ACAATGGGCA CTGGCCCCTC 
CGGGGAGAGA AGGTGATCCG CTACCGGGTG GTGAACATCT ATGACGACAA GGGTCTGAAC 
CCCACGGTGA GTGAGGAGCA GATCCGTCGG CAGCACGAGG TGCTCAATGA GGCCTTCAGC 
CGCTACAACA TCAGCTGGCA GCTGAGCGTC CACCGCGTCC ACAACTCCAC CCTGCGCCAC 
CGCATTGTGC TGGTGAACTG TGAGCCCAGC AAAATCGGCA ACGATCACTG TGACCCCGAG 
TGCGAGCATC CACTCACGGG CTACGACGGG GGAGACTGCC GCCTGCAGGG CCGGTGCTAC 
TCCTGGAACC GCAAGGACGG GCTCTGCCAC GTGGGGTGCA ACAACATGCT GAATGACTTT 
GACGAGGGCG ACTGCTGTGA CCCAGACGTG ACCGAGGTGC GCAAGACCTG CTTCGACCCT 
GACTCACCCA GGAGGGCATA CATGAGTGTG AAGGAGCTGA AGGAGGCACT GCAGCTCAAC 
AGCACCCACT TCCTCAATGT CTACTTTGCC AGCTCAGTAC GAGAAAACCT TGCAGGTGCT 
GCCACCTGGC CTTGGGACAA GGAAGCCATC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
GCCTATTATG GCATGCCTGG CCACACCAAC ATCATGATCC ATGAGGTGGG GCATGTCCTG 
GGACTCTACC ATGTCTTCAA AGGGGTCAGT GAGAGAGAAT CCTGCGATGA CCCCTGCAGG 
GAGACTGTGC CGTCCATGGA GACAGGAGAC CTCTGCGCCG ACACTGCCCC TACTCCCAAG 
AGTAAGCTGT GCCAGAACCC AGAGCCTGCC AATGACACCT GTGGCTTCAC CTACTTCCCA 
GGGGCTCCAT TCAACAACTA CATGAGTTAT ACAGACGATG ACTGCACTGA CAGCTTCACC 
CCCAACCAAG TGGCAAGAAT GCACTGCTAT CTGGACCTTG TGTATCAGCA GTGGAGCCAG 
AGCCAGAAGC CCACCCCCAT CCCCATCCCC CCCATGGTCA TCGGGCAGAC TCAGAAGTCC 
CTCACTATCC ACTGGCTGCC TCCGATTAGT GGAGTTATGT ATGACAGGGT CCCGGGCAGC 
ATGTGTGGCG CCTGCACTGA AGATGGGACG TTCCGTCAGT ACGTGCACAC AGCTTCCTCC 
CAGCGCGTGT GTGACTCCTC AGGTTACTGG ACTCCGGAGG AGGCTGTGGG GCCTCCGGAC 
GTGGATCAGC CTTGTGAGCC GAGCTTGCAG GCCTGGAGTC CCGAGCTCCA CCTGCACCAC 
ACGAACATGA CGGTCCCCTG CCCTGCGGAA GGCTGCAGCC TGGAGCTGCT CTTTCAGCAC 
CCAGTCCAAG CGGACACTCT CACCCTGTGG GTCACCTACC TCTCCATGGG CTCTTCCCCG 
GTGCTCTTTG ACACAGAGAT CTTGCTGGAA CACCAGAAGT CTGTGCACCT GGGCCCCTTG 
GACACTTTCT GTGACACACC GCTCACTATC AAACTGCATG TTGATGGAAA GGTCATGGGG 
GTGAAAGTCT ACACCTTTGA TGAGCGGATG GAGATTGACG CAGCTCTCCT GACTTCTCAG 
CCCCACAGTC CCCTGTGTTC TGGCTGCAGG CCTGTGAGCT ACCAGATTCT CCGCGAGCCT 
CCGTTTGCCA GCGGCTTGCC TGCAGCGGTG ATGCATCCTC ACCGGAAGTT CACAGACATG 
GAGGTCATGC CTGGGCAGAT GTATCGGTAC CAAGTTCAAG CTGCAGCTGA AGCAGAACTG 
GGAGAAGCTT CGCCTCCTCT GAGCCACATC CATGGAGCTC CTTACTGTGG AGATGGGAAG 
GTTGCAAGGA ACCTGGGAGA AGAGTGTGAT GATGGAGACC TTTTGAATGG AGACGGCTGC 
TCAAGGGCAT GTGAGTTAGA GGAAGGTTTC AACTGTGTAG GGGAACCAAG CCTGTGCTAT 
ATACACGAAG GAGATGGAAT ATGTGAGCCT TTTGAGAAGG AAACCAGCAT TGTAGACTGT 
GGCCTCTATA CTCCCAAAGG ATACCTGGAT CAGTGGGCTA CCCAAGCTTT TGCTTCTCAT 
GAAGACACGA AGAAATGTCC TGTTTCCTTG GTAACTGGAG CACCTCATTC CATGGTTTGC 
ACATCATACC GTCCAGATTT ACCAGAGCAC CCACCCCTAA CTGGCTGGTT TCCCTGTGTC 
CCCAGTGGAA ATAGATCTCA AGATGAAGGG AGTAAGCAGC CAAAAGGTAG CCTGAAGAGA 
GAGGATGAGG TTTGGCTCAA AGTTTGTTTC AGTAGACCAG GAGTGGCCAC AGCAATTTTC 
ATTTTCTTGA CATCTGATGG CCTGGTATCT GGGAAACGTC AGCGGCCAAC AGTGACCCTT 
TACCTGACCG ATGTCAGTGG AAACAACCAC TCTCTTGGAA CCTATGAACT GTCATGCCAG 
CATAACCCGC TGGTTATCAA CATAACTCAT CACTCAAATG TCCTCTTCCA CCATGCCACA 
TCAGTGCTGC TGAATTTCTC AACCCCGTTG GTGGGCATCT CAGCGGTGGC TCTAAGGACA 
TCCTCCGACA CAAGTCCTTC AGCTCCCAAC AGCTGCATCA TCCCAGAGCA AAAGGGGCAA 
AATCATCAGG GACAAAGCTG TGTCCATCGG CCCTGTGGGG AGCAGGGCAG CTGTGTGCCA 
TTGCTGCTTG ATCACGCGGA TATGGTGAAC TGTACCTCTG GTGGCCCGGG TCTCATGAAG 
TGCTCTATCA CCTGTCAGAG GGGGTCTGCC CTTCAGGCCA GCAATGGGCA GTACCTCAGG 
CCCCTGCAGA AGAAAATTCT GCTCACATGT TCCTCTGGGC ACTGGGACGG GGCTGTGAGC 
TGCCAACCCC TTGACTGTGG TGTCCCCGAC GCATCTTTGG TGAACTACGC AAATTTCTCC 
TGCTTGGAGG GAACCAACTT TCTGAAACGC TGCTCTATCT CCTGTATCCC ACCAGCCAAG 
CTTCAAGGAC TGAGCCCATG GCTGACCTGT CTTGAAGATG GGCTCTGGTC TCTCCCTGAA 
GCTTACTGCA AGCTGGAGTG TGATGCTCCT CCTGTTATCC CGAATGCCAA CCTGCTCCAG 
CCTCGCTGCC TCCAGGGGAA CCATGACGTG GGCTCTACCT GCAGATATGA ATGCAAACCG 
GGCTACTATG TGCTGGGAAG CACGGAGAAT AAAGTCAGGA ACAAGTCCCT GAAGATCCAG 
TGCCTGGAGA GTGGAATCTG GGAGCAAGGC AGCTGTGTCC CAGTGGTGTG CGAGCCCCCC 
TCCCCTGTCT TTGAAGGCAT GTATGAATGC ACCAATGGCT TTGAGCTGGA CAGCCAGTGT 
TTTATCAACT GTAACCAGGA AAGTAAAAGG CTTCCCATCC GCTGCACGAA GGAGGGACGG 
TGGAGCGAGG AGTTCAAGTT ATGTGAGAAC CTGCGAGGGG AATGCCCACC ACCCACCTCA 
GACCCCAAAT TTGTGGAGTA CAAGTGTGAA CAAGGACATG GGATAGGTGC AGTGTGTTCC 
CCATTGTGTA TCGTCCTCCC TAGTGATCCT GTAATCCTTC CTGAGAATAT CACTACTGAC 
ACTCTGGAGC ACTGGATGGA ACCCGTCAGA GTACAGAGTG TCATGTGTAC CGGACGGCGT 
CAGTGGCACC CAGACCCCCT CCTGATCCAC TGCATCCAGT CCTGCGAGCC TTTCCAAGGG 
GACGGTTGGT GTGACACCAT CAATAACCGG GCCTATTGCA ACTATGATGG GGGAGACTGC 
TGCCCTTCTA CACTCTCCTC CAAGAAGGTC ATTCCATTTG CTGCTGACTG TGATCAGGAT 
GAATGCACCT GCCGAGACCC CAGGGCTGAA GAAAACCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012042985.1
CDS	1198..6579
Translation	MMCFKILRVSLVVLAGWALSTVSSELGWTRKRSLAQRGYLNQVLSEGERCWLGAKVRRPRVAPQHHLFGVYPSLPGDYLRPYPAGDQGRAHVRCSEPDAGGMVVSSVPPDPTASARKRSEAEQPAAPWGGDSTIGQSELLGDDDTYLGDGGSKEPLGEAGTPEGTALAATVITSFPCPKESGPDTQRKGRTKTRRRRQVVKGQAGGLRGDPEFAPQHFQPWPKHPLTPRDRTGPSEGGIRNGGGDPSWEAETETVSPQGGLPVLYFSGRREPLLLRPEVLADIPREAFTVEAWVKPEGGQNSPAIIAGVFDNCSHTISDKGWALGIRSGKDVGRRDARFFFSLRTDRVKKATTVMGHSRYQPGKWTHLAATYDGQRMALYVDGTQVASSRDQSGPLNSPFMASCRSLLLGGDSSEDGHSFRGHLGTLVFWSTARPQSHLQHRPQQPLEVKDLTTLLLTASFEPLEEQWVPFRDGKYPRREVLQGFESKPEILSPLQPPLCGQTACDNVELISQYNGHWPLRGEKVIRYRVVNIYDDKGLNPTVSEEQIRRQHEVLNEAFSRYNISWQLSVHRVHNSTLRHRIVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRKDGLCHVGCNNMLNDFDEGDCCDPDVTEVRKTCFDPDSPRRAYMSVKELKEALQLNSTHFLNVYFASSVRENLAGAATWPWDKEAISHLGGVVLNPAYYGMPGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMETGDLCADTAPTPKSKLCQNPEPANDTCGFTYFPGAPFNNYMSYTDDDCTDSFTPNQVARMHCYLDLVYQQWSQSQKPTPIPIPPMVIGQTQKSLTIHWLPPISGVMYDRVPGSMCGACTEDGTFRQYVHTASSQRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLHHTNMTVPCPAEGCSLELLFQHPVQADTLTLWVTYLSMGSSPVLFDTEILLEHQKSVHLGPLDTFCDTPLTIKLHVDGKVMGVKVYTFDERMEIDAALLTSQPHSPLCSGCRPVSYQILREPPFASGLPAAVMHPHRKFTDMEVMPGQMYRYQVQAAAEAELGEASPPLSHIHGAPYCGDGKVARNLGEECDDGDLLNGDGCSRACELEEGFNCVGEPSLCYIHEGDGICEPFEKETSIVDCGLYTPKGYLDQWATQAFASHEDTKKCPVSLVTGAPHSMVCTSYRPDLPEHPPLTGWFPCVPSGNRSQDEGSKQPKGSLKREDEVWLKVCFSRPGVATAIFIFLTSDGLVSGKRQRPTVTLYLTDVSGNNHSLGTYELSCQHNPLVINITHHSNVLFHHATSVLLNFSTPLVGISAVALRTSSDTSPSAPNSCIIPEQKGQNHQGQSCVHRPCGEQGSCVPLLLDHADMVNCTSGGPGLMKCSITCQRGSALQASNGQYLRPLQKKILLTCSSGHWDGAVSCQPLDCGVPDASLVNYANFSCLEGTNFLKRCSISCIPPAKLQGLSPWLTCLEDGLWSLPEAYCKLECDAPPVIPNANLLQPRCLQGNHDVGSTCRYECKPGYYVLGSTENKVRNKSLKIQCLESGIWEQGSCVPVVCEPPSPVFEGMYECTNGFELDSQCFINCNQESKRLPIRCTKEGRWSEEFKLCENLRGECPPPTSDPKFVEYKCEQGHGIGAVCSPLCIVLPSDPVILPENITTDTLEHWMEPVRVQSVMCTGRRQWHPDPLLIHCIQSCEPFQGDGWCDTINNRAYCNYDGGDCCPSTLSSKKVIPFAADCDQDECTCRDPRAEENQ

Target ORF information:

RefSeq Version	XM_012187595.2
Organism	Ovis aries(sheep)
Definition	Ovis aries pappalysin 2 (PAPPA2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012187595.2

ORF Insert Sequence:

ATGATGTGCT TCAAGATTCT GAGGGTAAGC CTGGTAGTCC TGGCTGGGTG GGCGCTCAGT 
ACTGTCAGCT CAGAGCTGGG CTGGACGCGC AAGAGATCCT TGGCCCAAAG AGGGTACCTG 
AACCAGGTGC TGTCGGAAGG AGAGCGCTGT TGGCTGGGGG CCAAGGTTCG AAGGCCCAGA 
GTTGCTCCTC AGCATCACCT CTTTGGGGTC TATCCCAGCC TGCCTGGGGA CTACCTGAGG 
CCCTACCCTG CAGGAGATCA GGGAAGGGCC CATGTAAGAT GCAGTGAGCC GGATGCCGGG 
GGAATGGTTG TGAGCTCTGT GCCCCCAGAC CCAACTGCAA GTGCAAGAAA GAGGAGTGAG 
GCTGAGCAGC CAGCTGCTCC GTGGGGAGGG GACAGTACTA TTGGGCAGTC GGAGCTACTG 
GGAGATGATG ACACTTATCT TGGTGATGGA GGATCCAAGG AGCCTCTAGG TGAGGCTGGG 
ACTCCAGAAG GCACAGCCTT GGCTGCCACT GTGATCACCA GCTTCCCATG CCCAAAGGAA 
TCCGGACCAG ACACCCAAAG GAAGGGCCGG ACCAAGACCA GGCGTCGGCG CCAGGTGGTG 
AAGGGACAGG CAGGAGGCCT GCGGGGAGAC CCTGAGTTTG CGCCCCAGCA CTTCCAGCCG 
TGGCCTAAGC ATCCCCTCAC GCCTAGGGAT CGAACCGGCC CATCAGAGGG TGGCATCCGG 
AATGGTGGAG GGGACCCCTC CTGGGAAGCA GAGACCGAGA CTGTGAGCCC CCAAGGAGGA 
CTGCCTGTCT TGTACTTCTC TGGGAGGCGG GAGCCGCTGC TGCTGCGTCC AGAAGTGCTG 
GCTGACATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAAGCCGGA GGGAGGACAG 
AACAGCCCAG CCATCATCGC AGGTGTGTTT GATAACTGCT CCCACACTAT CAGTGACAAG 
GGCTGGGCCC TGGGGATCCG CTCGGGGAAG GATGTGGGAA GGCGAGATGC TCGCTTCTTC 
TTCTCTCTTC GCACCGACCG AGTGAAGAAA GCCACCACTG TGATGGGCCA CAGTCGCTAC 
CAACCGGGCA AGTGGACACA TCTGGCAGCC ACTTATGATG GACAGCGCAT GGCCTTGTAT 
GTGGATGGCA CTCAGGTGGC TAGTAGTCGA GATCAGTCTG GTCCCTTGAA CAGCCCCTTC 
ATGGCATCTT GTCGCTCTCT CCTCCTTGGG GGGGACAGCT CGGAGGACGG GCACTCTTTC 
CGTGGACACC TGGGCACCCT GGTCTTCTGG TCGACAGCCC GCCCACAAAG CCACCTTCAG 
CACAGACCTC AGCAGCCACT GGAGGTGAAG GATTTGACCA CCCTGCTACT GACCGCCAGT 
TTTGAGCCCC TGGAAGAACA GTGGGTCCCC TTTAGAGACG GGAAGTACCC ACGGCGTGAG 
GTTCTCCAGG GCTTTGAGTC AAAGCCTGAG ATTCTGTCCC CTCTGCAGCC CCCGCTCTGT 
GGGCAGACAG CATGTGACAA TGTGGAATTG ATCTCTCAGT ACAATGGGCA CTGGCCCCTC 
CGGGGAGAGA AGGTGATCCG CTACCGGGTG GTGAACATCT ATGACGACAA GGGTCTGAAC 
CCCACGGTGA GTGAGGAGCA GATCCGTCGG CAGCACGAGG TGCTCAATGA GGCCTTCAGC 
CGCTACAACA TCAGCTGGCA GCTGAGCGTC CACCGCGTCC ACAACTCCAC CCTGCGCCAC 
CGCATTGTGC TGGTGAACTG TGAGCCCAGC AAAATCGGCA ACGATCACTG TGACCCCGAG 
TGCGAGCATC CACTCACGGG CTACGACGGG GGAGACTGCC GCCTGCAGGG CCGGTGCTAC 
TCCTGGAACC GCAAGGACGG GCTCTGCCAC GTGGGGTGCA ACAACATGCT GAATGACTTT 
GACGAGGGCG ACTGCTGTGA CCCAGACGTG ACCGAGGTGC GCAAGACCTG CTTCGACCCT 
GACTCACCCA GGAGGGCATA CATGAGTGTG AAGGAGCTGA AGGAGGCACT GCAGCTCAAC 
AGCACCCACT TCCTCAATGT CTACTTTGCC AGCTCAGTAC GAGAAAACCT TGCAGGTGCT 
GCCACCTGGC CTTGGGACAA GGAAGCCATC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
GCCTATTATG GCATGCCTGG CCACACCAAC ATCATGATCC ATGAGGTGGG GCATGTCCTG 
GGACTCTACC ATGTCTTCAA AGGGGTCAGT GAGAGAGAAT CCTGCGATGA CCCCTGCAGG 
GAGACTGTGC CGTCCATGGA GACAGGAGAC CTCTGCGCCG ACACTGCCCC TACTCCCAAG 
AGTAAGCTGT GCCAGAACCC AGAGCCTGCC AATGACACCT GTGGCTTCAC CTACTTCCCA 
GGGGCTCCAT TCAACAACTA CATGAGTTAT ACAGACGATG ACTGCACTGA CAGCTTCACC 
CCCAACCAAG TGGCAAGAAT GCACTGCTAT CTGGACCTTG TGTATCAGCA GTGGAGCCAG 
AGCCAGAAGC CCACCCCCAT CCCCATCCCC CCCATGGTCA TCGGGCAGAC TCAGAAGTCC 
CTCACTATCC ACTGGCTGCC TCCGATTAGT GGAGTTATGT ATGACAGGGT CCCGGGCAGC 
ATGTGTGGCG CCTGCACTGA AGATGGGACG TTCCGTCAGT ACGTGCACAC AGCTTCCTCC 
CAGCGCGTGT GTGACTCCTC AGGTTACTGG ACTCCGGAGG AGGCTGTGGG GCCTCCGGAC 
GTGGATCAGC CTTGTGAGCC GAGCTTGCAG GCCTGGAGTC CCGAGCTCCA CCTGCACCAC 
ACGAACATGA CGGTCCCCTG CCCTGCGGAA GGCTGCAGCC TGGAGCTGCT CTTTCAGCAC 
CCAGTCCAAG CGGACACTCT CACCCTGTGG GTCACCTACC TCTCCATGGG CTCTTCCCCG 
GTGCTCTTTG ACACAGAGAT CTTGCTGGAA CACCAGAAGT CTGTGCACCT GGGCCCCTTG 
GACACTTTCT GTGACACACC GCTCACTATC AAACTGCATG TTGATGGAAA GGTCATGGGG 
GTGAAAGTCT ACACCTTTGA TGAGCGGATG GAGATTGACG CAGCTCTCCT GACTTCTCAG 
CCCCACAGTC CCCTGTGTTC TGGCTGCAGG CCTGTGAGCT ACCAGATTCT CCGCGAGCCT 
CCGTTTGCCA GCGGCTTGCC TGCAGCGGTG ATGCATCCTC ACCGGAAGTT CACAGACATG 
GAGGTCATGC CTGGGCAGAT GTATCGGTAC CAAGTTCAAG CTGCAGCTGA AGCAGAACTG 
GGAGAAGCTT CGCCTCCTCT GAGCCACATC CATGGAGCTC CTTACTGTGG AGATGGGAAG 
GTTGCAAGGA ACCTGGGAGA AGAGTGTGAT GATGGAGACC TTTTGAATGG AGACGGCTGC 
TCAAGGGCAT GTGAGTTAGA GGAAGGTTTC AACTGTGTAG GGGAACCAAG CCTGTGCTAT 
ATACACGAAG GAGATGGAAT ATGTGAGCCT TTTGAGAAGG AAACCAGCAT TGTAGACTGT 
GGCCTCTATA CTCCCAAAGG ATACCTGGAT CAGTGGGCTA CCCAAGCTTT TGCTTCTCAT 
GAAGACACGA AGAAATGTCC TGTTTCCTTG GTAACTGGAG CACCTCATTC CATGGTTTGC 
ACATCATACC GTCCAGATTT ACCAGAGCAC CCACCCCTAA CTGGCTGGTT TCCCTGTGTC 
CCCAGTGGAA ATAGATCTCA AGATGAAGGG AGTAAGCAGC CAAAAGGTAG CCTGAAGAGA 
GAGGATGAGG TTTGGCTCAA AGTTTGTTTC AGTAGACCAG GAGTGGCCAC AGCAATTTTC 
ATTTTCTTGA CATCTGATGG CCTGGTATCT GGGAAACGTC AGCGGCCAAC AGTGACCCTT 
TACCTGACCG ATGTCAGTGG AAACAACCAC TCTCTTGGAA CCTATGAACT GTCATGCCAG 
CATAACCCGC TGGTTATCAA CATAACTCAT CACTCAAATG TCCTCTTCCA CCATGCCACA 
TCAGTGCTGC TGAATTTCTC AACCCCGTTG GTGGGCATCT CAGCGGTGGC TCTAAGGACA 
TCCTCCGACA CAAGTCCTTC AGCTCCCAAC AGCTGCATCA TCCCAGAGCA AAAGGGGCAA 
AATCATCAGG GACAAAGCTG TGTCCATCGG CCCTGTGGGG AGCAGGGCAG CTGTGTGCCA 
TTGCTGCTTG ATCACGCGGA TATGGTGAAC TGTACCTCTG GTGGCCCGGG TCTCATGAAG 
TGCTCTATCA CCTGTCAGAG GGGGTCTGCC CTTCAGGCCA GCAATGGGCA GTACCTCAGG 
CCCCTGCAGA AGAAAATTCT GCTCACATGT TCCTCTGGGC ACTGGGACGG GGCTGTGAGC 
TGCCAACCCC TTGACTGTGG TGTCCCCGAC GCATCTTTGG TGAACTACGC AAATTTCTCC 
TGCTTGGAGG GAACCAACTT TCTGAAACGC TGCTCTATCT CCTGTATCCC ACCAGCCAAG 
CTTCAAGGAC TGAGCCCATG GCTGACCTGT CTTGAAGATG GGCTCTGGTC TCTCCCTGAA 
GCTTACTGCA AGCTGGAGTG TGATGCTCCT CCTGTTATCC CGAATGCCAA CCTGCTCCAG 
CCTCGCTGCC TCCAGGGGAA CCATGACGTG GGCTCTACCT GCAGATATGA ATGCAAACCG 
GGCTACTATG TGCTGGGAAG CACGGAGAAT AAAGTCAGGA ACAAGTCCCT GAAGATCCAG 
TGCCTGGAGA GTGGAATCTG GGAGCAAGGC AGCTGTGTCC CAGTGGTGTG CGAGCCCCCC 
TCCCCTGTCT TTGAAGGCAT GTATGAATGC ACCAATGGCT TTGAGCTGGA CAGCCAGTGT 
TTTATCAACT GTAACCAGGA AAGTAAAAGG CTTCCCATCC GCTGCACGAA GGAGGGACGG 
TGGAGCGAGG AGTTCAAGTT ATGTGAGAAC CTGCGAGGGG AATGCCCACC ACCCACCTCA 
GACCCCAAAT TTGTGGAGTA CAAGTGTGAA CAAGGACATG GGATAGGTGC AGTGTGTTCC 
CCATTGTGTA TCGTCCTCCC TAGTGATCCT GTAATCCTTC CTGAGAATAT CACTACTGAC 
ACTCTGGAGC ACTGGATGGA ACCCGTCAGA GTACAGAGTG TCATGTGTAC CGGACGGCGT 
CAGTGGCACC CAGACCCCCT CCTGATCCAC TGCATCCAGT CCTGCGAGCC TTTCCAAGGG 
GACGGTTGGT GTGACACCAT CAATAACCGG GCCTATTGCA ACTATGATGG GGGAGACTGC 
TGCCCTTCTA CACTCTCCTC CAAGAAGGTC ATTCCATTTG CTGCTGACTG TGATCAGGAT 
GAATGCACCT GCCGAGACCC CAGGGCTGAA GAAAACCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( XM_004013823.4 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004013872.2 Ovis aries pappalysin 2 (PAPPA2), mRNA.

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CloneID	OSm61874
Clone ID Related Accession (Same CDS sequence)	XM_004013823.4
Accession Version	XM_004013823.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549296000000
Organism	Ovis aries(sheep)
Product	pappalysin-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040263.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_004013823.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCT TCAAGATTCT GAGGGTAAGC CTGGTAGTCC TGGCTGGGTG GGCGCTCAGT 
ACTGTCAGCT CAGAGCTGGG CTGGACGCGC AAGAGATCCT TGGCCCAAAG AGGGTACCTG 
AACCAGGTGC TGTCGGAAGG AGAGCGCTGT TGGCTGGGGG CCAAGGTTCG AAGGCCCAGA 
GTTGCTCCTC AGCATCACCT CTTTGGGGTC TATCCCAGCC TGCCTGGGGA CTACCTGAGG 
CCCTACCCTG CAGGAGATCA GGGAAGGGCC CATGTAAGAT GCAGTGAGCC GGATGCCGGG 
GGAATGGTTG TGAGCTCTGT GCCCCCAGAC CCAACTGCAA GTGCAAGAAA GAGGAGTGAG 
GCTGAGCAGC CAGCTGCTCC GTGGGGAGGG GACAGTACTA TTGGGCAGTC GGAGCTACTG 
GGAGATGATG ACACTTATCT TGGTGATGGA GGATCCAAGG AGCCTCTAGG TGAGGCTGGG 
ACTCCAGAAG GCACAGCCTT GGCTGCCACT GTGATCACCA GCTTCCCATG CCCAAAGGAA 
TCCGGACCAG ACACCCAAAG GAAGGGCCGG ACCAAGACCA GGCGTCGGCG CCAGGTGGTG 
AAGGGACAGG CAGGAGGCCT GCGGGGAGAC CCTGAGTTTG CGCCCCAGCA CTTCCAGCCG 
TGGCCTAAGC ATCCCCTCAC GCCTAGGGAT CGAACCGGCC CATCAGAGGG TGGCATCCGG 
AATGGTGGAG GGGACCCCTC CTGGGAAGCA GAGACCGAGA CTGTGAGCCC CCAAGGAGGA 
CTGCCTGTCT TGTACTTCTC TGGGAGGCGG GAGCCGCTGC TGCTGCGTCC AGAAGTGCTG 
GCTGACATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAAGCCGGA GGGAGGACAG 
AACAGCCCAG CCATCATCGC AGGTGTGTTT GATAACTGCT CCCACACTAT CAGTGACAAG 
GGCTGGGCCC TGGGGATCCG CTCGGGGAAG GATGTGGGAA GGCGAGATGC TCGCTTCTTC 
TTCTCTCTTC GCACCGACCG AGTGAAGAAA GCCACCACTG TGATGGGCCA CAGTCGCTAC 
CAACCGGGCA AGTGGACACA TCTGGCAGCC ACTTACGATG GACAGCGCAT GGCCTTGTAT 
GTGGATGGCA CTCAGGTGGC TAGTAGTCGA CATCAGTCTG GTCCCTTGAA CAGCCCCTTC 
ATGGCATCTT GTCGCTCTCT CCTCCTTGGG GGGGACAGCT CGGAGGACGG GCACTCTTTC 
CGTGGACACC TGGGCACCCT GGTCTTCTGG TCGACAGCCC GCCCACAAAG CCACCTTCAG 
CACAGACCTC AGCAGCCACT GGAGGTGAAG GATTTGACCA CCCTGCTACT GACCGCCAGT 
TTTGAGCCCC TGGAAGAACA GTGGGTCCCC TTTAGAGACG GGAAGTACCC ACGGCGTGAG 
GTTCTCCAGG GCTTTGAGTC AAAGCCTGAG ATTCTGTCCC CTCTGCAGCC CCCGCTCTGT 
GGGCAGACAG CATGTGACAA TGTGGAATTG ATCTCTCAGT ACAATGGGCA CTGGCCCCTC 
CGGGGAGAGA AGGTGATCCG CTACCGGGTG GTGAACATCT ATGACGACAA GGGTCTGAAC 
CCCACGGTGA GTGAGGAGCA GATCCGTCGG CAGCACGAGG TGCTCAATGA GGCCTTCAGC 
CGCTACAACA TCAGCTGGCA GCTGAGCGTC CACCGCGTCC ACAACTCCAC CCTGCGCCAC 
CGCATTGTGC TGGTGAACTG TGAGCCCAGC AAAATCGGCA ACGATCACTG TGACCCCGAG 
TGCGAGCATC CACTCACGGG CTACGACGGG GGAGACTGCC GCCTGCAGGG CCGGTGCTAC 
TCCTGGAACC GCAAGGACGG GCTCTGCCAC GTGGGGTGCA ACAACATGCT GAATGACTTT 
GACGAGGGCG ACTGCTGTGA CCCAGACGTG ACCGAGGTGC GCAAGACCTG CTTCGACCCT 
GACTCACCCA GGAGGGCATA CATGAGTGTG AAGGAGCTGA AGGAGGCACT GCAGCTCAAC 
AGCACCCACT TCCTCAATGT CTACTTTGCC AGCTCAGTAC GAGAAAACCT TGCAGGTGCT 
GCCACCTGGC CTTGGGACAA GGAAGCCATC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
GCCTATTATG GCATGCCTGG CCACACCAAC ATCATGATCC ATGAGGTGGG GCATGTCCTG 
GGACTCTACC ATGTCTTCAA AGGGGTCAGT GAGAGAGAAT CCTGCGATGA CCCCTGCAGG 
GAGACTGTGC CGTCCATGGA GACAGGAGAC CTCTGCGCCG ACACTGCCCC TACTCCCAAG 
AGTAAGCTGT GCCAGAACCC AGAGCCTGCC AATGACACCT GTGGCTTCAC CTACTTCCCA 
GGGGCTCCAT TCAACAACTA CATGAGTTAT ACAGACGATG ACTGCACTGA CAGCTTCACC 
CCCAACCAAG TGGCACGAAT GCACTGCTAT CTGGACCTTG TGTATCAGCA GTGGAGCCAG 
AGCCAGAAGC CCACCCCCAT CCCCATCCCC CCCATGGTCA TCGGGCAGAC TCAGAAGTCC 
CTCACTATCC ACTGGCTGCC TCCGATTAGT GGAGTTATGT ATGACAGGGT CCCGGGCAGC 
ATGTGTGGCG CCTGCACTGA AGATGGGACG TTCCGTCAGT ACGTGCACAC AGCTTCCTCC 
CAGCGCGTGT GTGACTCCTC AGGTTACTGG ACTCCGGAGG AGGCTGTGGG GCCTCCGGAC 
GTGGATCAGC CTTGTGAGCC GAGCTTGCAG GCCTGGAGTC CCGAGCTCCA CCTGCACCAC 
ACGAACATGA CGGTCCCCTG CCCTGCGGAA GGCTGCAGCC TGGAGCTGCT CTTTCAGCAC 
CCAGTCCAAG CGGACACTCT CACCCTGTGG GTCACCTACC TCTCCATGGG CTCTTCCCCG 
GTGCTCTTTG ACACAGAGAT CTTGCTGGAA CACCAGAAGT CTGTGCACTT GGGCCCCTTG 
GACACTTTCT GTGACACACC GCTCACTATC AAACTGCATG TTGATGGAAA GGTCATGGGG 
GTGAAAGTCT ACACCTTTGA TGAGCGGATG GAGATTGACG CAGCTCTCCT GACTTCTCAG 
CCCCACAGTC CCCTGTGTTC TGGCTGCAGG CCTGTGAGCT ACCAGATTCT GCGCGAGCCT 
CCGTTTGCCA GCGGCTTGCC TGCAGCGGTG ATGCATCCTC ACCGGAAGTT CACAGACATG 
GAGGTCATGC CTGGGCAGAT GTATCGGTAC CAAGTTCAAG CTGCAGCTGA AGCAGAACTG 
GGAGAAGCTT CGCCTCCTCT GAGCCACATC CATGGAGCTC CTTACTGTGG AGATGGGAAG 
GTTGCAAGGA ACCTGGGAGA AGAGTGTGAT GATGGAGACC TTTTGAATGG AGACGGCTGC 
TCAAGGGCAT GTGAGTTAGA GGAAGGTTTC AACTGTGTAG GGGAACCAAG CCTGTGCTAT 
ATACACGAGG GAGATGGAAT ATGTGAGCCT TTTGAGAAGG AAACCAGCAT TGTAGACTGT 
GGCCTCTATA CTCCCAAAGG ATACCTGGAT CAGTGGGCTA CCCAAGCTTT TGCTTCTCAT 
GAAGACACGA AGAAATGTCC TGTTTCCTTG GTAACTGGAG CACCTCATTC CATGGTTTGC 
ACATCATACC GTCCAGATTT ACCAGAGCAC CGACCCCTAA CTGGCTGGTT TCCCTGTGTC 
CCCAGTGGAA ATAGATCTCA AGATGAAGGG AGTAAGCAGC CAAAAGGTAG CCTGAAGAGA 
GAGGATGAGG TTTGGCTCAA AGTTTGTTTC AGTAGACCAG GAGTGGCCAC AGCAATTTTC 
ATTTTCTTGA CATCTGATGG CCTGGTATCT GGGAAACGTC AGCGGCCAAC AGTGACCCTT 
TACCTGACCG ATGTCAGTGG AAACAACCAC TCTCTTGGAA CCTATGAACT GTCATGCCAG 
CATAACCCGC TGGTTATCAA CATAACTCAT CACTCAAATG TCCTCTTCCA CCATGCCACA 
TCAGTGCTGC TGAATTTCTC AACCCCGTTG GTGGGCATCT CAGCGGTGGC TCTAAGGACA 
TCCTCCGACA CAAGTCCTTC AGCTCCCAAC AGCTGCATCA TCCCAGAGCA AAAGGGGCAA 
AATCATCAGG GACAAAGCTG TGTCCATCGG CCCTGTGGGG AGCAGGGCAG CTGTGTGCCA 
TTGCTGCTTG ATCACGCGGA TATGGTGAAC TGTACCTCTG GTGGCCCGGG TCTCATGAAG 
TGCTCTATCA CCTGTCAGAG GGGGTCTGCC CTTCAGGCCA GCAATGGGCA GTACCTCAGG 
CCCATGCAGA AGAAAATTCT GCTCACATGT TCCTCTGGGC ACTGGGACCG GGCTGTGAGC 
TGCCAACCCC TTGACTGTGG TGTCCCCGAC GCATCTTTGG TGAACTACGC AAATTTCTCC 
TGCTTGGAGG GAACCAACTT TCTGAAACGC TGCTCTATCT CCTGTATCCC ACCAGCCAAG 
CTTCAAGGAC TGAGCCCATG GCTGACCTGT CTTGAAGATG GGCTCTGGTC TCTCCCTGAA 
GCTTACTGCA AGCTGGAGTG TGATGCTCCT CCTGTTATCC CGAATGCCAA CCTGCTCCAG 
CCTCGCTGCC TCCAGGGGAA CCATGACGTG GGCTCTACCT GCAGATATGA ATGCAAACCG 
GGCTACTATG TGCTGGGAAG CACAGAGAAT AAAGTCAGGA ACAAGTCCCT GAAGATCCAG 
TGCCTGGAGA GTGGAATCTG GGAGCAAGGC AGCTGTGTCC CAGTGGTGTG CGAGCCCCCC 
TCCCCTGTCT TTGAAGGCAT GTATGAATGC ACCAATGGCT TTGAGCTCGA CAGCCAGTGT 
TTTATCAACT GTAACCAGGA AAGTAAAAGG CTTCCCATCC GCTGCACGAA GGAGGGACAG 
TGGAGCGAGG AGTTCAAGTT ATGTGAGAAC CTGCGAGGGG AATGCCCACC ACCCACCTCA 
GACCCCAAAT TTGTGGAGTA CAAGTGTGAA CAAGGACACG GGATAGGTGC AGTGTGTTCC 
CCATTGTGTA TCGTCCTCCC TAGTGATCCT GTAATCCTTC CTGAGAATGT CACTACTGAC 
ACTCTGGAGC ACTGGATGGA ACCCGTCAGA GTACAGAGTG TCATGTGTAC TGGACGGCGT 
CAGTGGCACC CAGACCCCCT CCTGATCCAC TGCATCCAGT CCTGCGAGCC TTTCCAAGGG 
GACGGTTGGT GTGACACCAT CAATAACCGG GCCTATTGCA ACTATGATGG GGGAGACTGC 
TGCCCTTCTA CACTCTCCTC CAAGAAGGTC ATTCCATTTG CTGCTGACTG TGATCAGGAT 
GAATGCACCT GCCGAGACCC CAGGGCTGAA GAAAACCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004013872.2
CDS	934..6315
Translation	MMCFKILRVSLVVLAGWALSTVSSELGWTRKRSLAQRGYLNQVLSEGERCWLGAKVRRPRVAPQHHLFGVYPSLPGDYLRPYPAGDQGRAHVRCSEPDAGGMVVSSVPPDPTASARKRSEAEQPAAPWGGDSTIGQSELLGDDDTYLGDGGSKEPLGEAGTPEGTALAATVITSFPCPKESGPDTQRKGRTKTRRRRQVVKGQAGGLRGDPEFAPQHFQPWPKHPLTPRDRTGPSEGGIRNGGGDPSWEAETETVSPQGGLPVLYFSGRREPLLLRPEVLADIPREAFTVEAWVKPEGGQNSPAIIAGVFDNCSHTISDKGWALGIRSGKDVGRRDARFFFSLRTDRVKKATTVMGHSRYQPGKWTHLAATYDGQRMALYVDGTQVASSRHQSGPLNSPFMASCRSLLLGGDSSEDGHSFRGHLGTLVFWSTARPQSHLQHRPQQPLEVKDLTTLLLTASFEPLEEQWVPFRDGKYPRREVLQGFESKPEILSPLQPPLCGQTACDNVELISQYNGHWPLRGEKVIRYRVVNIYDDKGLNPTVSEEQIRRQHEVLNEAFSRYNISWQLSVHRVHNSTLRHRIVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRKDGLCHVGCNNMLNDFDEGDCCDPDVTEVRKTCFDPDSPRRAYMSVKELKEALQLNSTHFLNVYFASSVRENLAGAATWPWDKEAISHLGGVVLNPAYYGMPGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMETGDLCADTAPTPKSKLCQNPEPANDTCGFTYFPGAPFNNYMSYTDDDCTDSFTPNQVARMHCYLDLVYQQWSQSQKPTPIPIPPMVIGQTQKSLTIHWLPPISGVMYDRVPGSMCGACTEDGTFRQYVHTASSQRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLHHTNMTVPCPAEGCSLELLFQHPVQADTLTLWVTYLSMGSSPVLFDTEILLEHQKSVHLGPLDTFCDTPLTIKLHVDGKVMGVKVYTFDERMEIDAALLTSQPHSPLCSGCRPVSYQILREPPFASGLPAAVMHPHRKFTDMEVMPGQMYRYQVQAAAEAELGEASPPLSHIHGAPYCGDGKVARNLGEECDDGDLLNGDGCSRACELEEGFNCVGEPSLCYIHEGDGICEPFEKETSIVDCGLYTPKGYLDQWATQAFASHEDTKKCPVSLVTGAPHSMVCTSYRPDLPEHRPLTGWFPCVPSGNRSQDEGSKQPKGSLKREDEVWLKVCFSRPGVATAIFIFLTSDGLVSGKRQRPTVTLYLTDVSGNNHSLGTYELSCQHNPLVINITHHSNVLFHHATSVLLNFSTPLVGISAVALRTSSDTSPSAPNSCIIPEQKGQNHQGQSCVHRPCGEQGSCVPLLLDHADMVNCTSGGPGLMKCSITCQRGSALQASNGQYLRPMQKKILLTCSSGHWDRAVSCQPLDCGVPDASLVNYANFSCLEGTNFLKRCSISCIPPAKLQGLSPWLTCLEDGLWSLPEAYCKLECDAPPVIPNANLLQPRCLQGNHDVGSTCRYECKPGYYVLGSTENKVRNKSLKIQCLESGIWEQGSCVPVVCEPPSPVFEGMYECTNGFELDSQCFINCNQESKRLPIRCTKEGQWSEEFKLCENLRGECPPPTSDPKFVEYKCEQGHGIGAVCSPLCIVLPSDPVILPENVTTDTLEHWMEPVRVQSVMCTGRRQWHPDPLLIHCIQSCEPFQGDGWCDTINNRAYCNYDGGDCCPSTLSSKKVIPFAADCDQDECTCRDPRAEENQ

Target ORF information:

RefSeq Version	XM_004013823.4
Organism	Ovis aries(sheep)
Definition	Ovis aries pappalysin 2 (PAPPA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004013823.4

ORF Insert Sequence:

ATGATGTGCT TCAAGATTCT GAGGGTAAGC CTGGTAGTCC TGGCTGGGTG GGCGCTCAGT 
ACTGTCAGCT CAGAGCTGGG CTGGACGCGC AAGAGATCCT TGGCCCAAAG AGGGTACCTG 
AACCAGGTGC TGTCGGAAGG AGAGCGCTGT TGGCTGGGGG CCAAGGTTCG AAGGCCCAGA 
GTTGCTCCTC AGCATCACCT CTTTGGGGTC TATCCCAGCC TGCCTGGGGA CTACCTGAGG 
CCCTACCCTG CAGGAGATCA GGGAAGGGCC CATGTAAGAT GCAGTGAGCC GGATGCCGGG 
GGAATGGTTG TGAGCTCTGT GCCCCCAGAC CCAACTGCAA GTGCAAGAAA GAGGAGTGAG 
GCTGAGCAGC CAGCTGCTCC GTGGGGAGGG GACAGTACTA TTGGGCAGTC GGAGCTACTG 
GGAGATGATG ACACTTATCT TGGTGATGGA GGATCCAAGG AGCCTCTAGG TGAGGCTGGG 
ACTCCAGAAG GCACAGCCTT GGCTGCCACT GTGATCACCA GCTTCCCATG CCCAAAGGAA 
TCCGGACCAG ACACCCAAAG GAAGGGCCGG ACCAAGACCA GGCGTCGGCG CCAGGTGGTG 
AAGGGACAGG CAGGAGGCCT GCGGGGAGAC CCTGAGTTTG CGCCCCAGCA CTTCCAGCCG 
TGGCCTAAGC ATCCCCTCAC GCCTAGGGAT CGAACCGGCC CATCAGAGGG TGGCATCCGG 
AATGGTGGAG GGGACCCCTC CTGGGAAGCA GAGACCGAGA CTGTGAGCCC CCAAGGAGGA 
CTGCCTGTCT TGTACTTCTC TGGGAGGCGG GAGCCGCTGC TGCTGCGTCC AGAAGTGCTG 
GCTGACATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAAGCCGGA GGGAGGACAG 
AACAGCCCAG CCATCATCGC AGGTGTGTTT GATAACTGCT CCCACACTAT CAGTGACAAG 
GGCTGGGCCC TGGGGATCCG CTCGGGGAAG GATGTGGGAA GGCGAGATGC TCGCTTCTTC 
TTCTCTCTTC GCACCGACCG AGTGAAGAAA GCCACCACTG TGATGGGCCA CAGTCGCTAC 
CAACCGGGCA AGTGGACACA TCTGGCAGCC ACTTACGATG GACAGCGCAT GGCCTTGTAT 
GTGGATGGCA CTCAGGTGGC TAGTAGTCGA CATCAGTCTG GTCCCTTGAA CAGCCCCTTC 
ATGGCATCTT GTCGCTCTCT CCTCCTTGGG GGGGACAGCT CGGAGGACGG GCACTCTTTC 
CGTGGACACC TGGGCACCCT GGTCTTCTGG TCGACAGCCC GCCCACAAAG CCACCTTCAG 
CACAGACCTC AGCAGCCACT GGAGGTGAAG GATTTGACCA CCCTGCTACT GACCGCCAGT 
TTTGAGCCCC TGGAAGAACA GTGGGTCCCC TTTAGAGACG GGAAGTACCC ACGGCGTGAG 
GTTCTCCAGG GCTTTGAGTC AAAGCCTGAG ATTCTGTCCC CTCTGCAGCC CCCGCTCTGT 
GGGCAGACAG CATGTGACAA TGTGGAATTG ATCTCTCAGT ACAATGGGCA CTGGCCCCTC 
CGGGGAGAGA AGGTGATCCG CTACCGGGTG GTGAACATCT ATGACGACAA GGGTCTGAAC 
CCCACGGTGA GTGAGGAGCA GATCCGTCGG CAGCACGAGG TGCTCAATGA GGCCTTCAGC 
CGCTACAACA TCAGCTGGCA GCTGAGCGTC CACCGCGTCC ACAACTCCAC CCTGCGCCAC 
CGCATTGTGC TGGTGAACTG TGAGCCCAGC AAAATCGGCA ACGATCACTG TGACCCCGAG 
TGCGAGCATC CACTCACGGG CTACGACGGG GGAGACTGCC GCCTGCAGGG CCGGTGCTAC 
TCCTGGAACC GCAAGGACGG GCTCTGCCAC GTGGGGTGCA ACAACATGCT GAATGACTTT 
GACGAGGGCG ACTGCTGTGA CCCAGACGTG ACCGAGGTGC GCAAGACCTG CTTCGACCCT 
GACTCACCCA GGAGGGCATA CATGAGTGTG AAGGAGCTGA AGGAGGCACT GCAGCTCAAC 
AGCACCCACT TCCTCAATGT CTACTTTGCC AGCTCAGTAC GAGAAAACCT TGCAGGTGCT 
GCCACCTGGC CTTGGGACAA GGAAGCCATC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
GCCTATTATG GCATGCCTGG CCACACCAAC ATCATGATCC ATGAGGTGGG GCATGTCCTG 
GGACTCTACC ATGTCTTCAA AGGGGTCAGT GAGAGAGAAT CCTGCGATGA CCCCTGCAGG 
GAGACTGTGC CGTCCATGGA GACAGGAGAC CTCTGCGCCG ACACTGCCCC TACTCCCAAG 
AGTAAGCTGT GCCAGAACCC AGAGCCTGCC AATGACACCT GTGGCTTCAC CTACTTCCCA 
GGGGCTCCAT TCAACAACTA CATGAGTTAT ACAGACGATG ACTGCACTGA CAGCTTCACC 
CCCAACCAAG TGGCACGAAT GCACTGCTAT CTGGACCTTG TGTATCAGCA GTGGAGCCAG 
AGCCAGAAGC CCACCCCCAT CCCCATCCCC CCCATGGTCA TCGGGCAGAC TCAGAAGTCC 
CTCACTATCC ACTGGCTGCC TCCGATTAGT GGAGTTATGT ATGACAGGGT CCCGGGCAGC 
ATGTGTGGCG CCTGCACTGA AGATGGGACG TTCCGTCAGT ACGTGCACAC AGCTTCCTCC 
CAGCGCGTGT GTGACTCCTC AGGTTACTGG ACTCCGGAGG AGGCTGTGGG GCCTCCGGAC 
GTGGATCAGC CTTGTGAGCC GAGCTTGCAG GCCTGGAGTC CCGAGCTCCA CCTGCACCAC 
ACGAACATGA CGGTCCCCTG CCCTGCGGAA GGCTGCAGCC TGGAGCTGCT CTTTCAGCAC 
CCAGTCCAAG CGGACACTCT CACCCTGTGG GTCACCTACC TCTCCATGGG CTCTTCCCCG 
GTGCTCTTTG ACACAGAGAT CTTGCTGGAA CACCAGAAGT CTGTGCACTT GGGCCCCTTG 
GACACTTTCT GTGACACACC GCTCACTATC AAACTGCATG TTGATGGAAA GGTCATGGGG 
GTGAAAGTCT ACACCTTTGA TGAGCGGATG GAGATTGACG CAGCTCTCCT GACTTCTCAG 
CCCCACAGTC CCCTGTGTTC TGGCTGCAGG CCTGTGAGCT ACCAGATTCT GCGCGAGCCT 
CCGTTTGCCA GCGGCTTGCC TGCAGCGGTG ATGCATCCTC ACCGGAAGTT CACAGACATG 
GAGGTCATGC CTGGGCAGAT GTATCGGTAC CAAGTTCAAG CTGCAGCTGA AGCAGAACTG 
GGAGAAGCTT CGCCTCCTCT GAGCCACATC CATGGAGCTC CTTACTGTGG AGATGGGAAG 
GTTGCAAGGA ACCTGGGAGA AGAGTGTGAT GATGGAGACC TTTTGAATGG AGACGGCTGC 
TCAAGGGCAT GTGAGTTAGA GGAAGGTTTC AACTGTGTAG GGGAACCAAG CCTGTGCTAT 
ATACACGAGG GAGATGGAAT ATGTGAGCCT TTTGAGAAGG AAACCAGCAT TGTAGACTGT 
GGCCTCTATA CTCCCAAAGG ATACCTGGAT CAGTGGGCTA CCCAAGCTTT TGCTTCTCAT 
GAAGACACGA AGAAATGTCC TGTTTCCTTG GTAACTGGAG CACCTCATTC CATGGTTTGC 
ACATCATACC GTCCAGATTT ACCAGAGCAC CGACCCCTAA CTGGCTGGTT TCCCTGTGTC 
CCCAGTGGAA ATAGATCTCA AGATGAAGGG AGTAAGCAGC CAAAAGGTAG CCTGAAGAGA 
GAGGATGAGG TTTGGCTCAA AGTTTGTTTC AGTAGACCAG GAGTGGCCAC AGCAATTTTC 
ATTTTCTTGA CATCTGATGG CCTGGTATCT GGGAAACGTC AGCGGCCAAC AGTGACCCTT 
TACCTGACCG ATGTCAGTGG AAACAACCAC TCTCTTGGAA CCTATGAACT GTCATGCCAG 
CATAACCCGC TGGTTATCAA CATAACTCAT CACTCAAATG TCCTCTTCCA CCATGCCACA 
TCAGTGCTGC TGAATTTCTC AACCCCGTTG GTGGGCATCT CAGCGGTGGC TCTAAGGACA 
TCCTCCGACA CAAGTCCTTC AGCTCCCAAC AGCTGCATCA TCCCAGAGCA AAAGGGGCAA 
AATCATCAGG GACAAAGCTG TGTCCATCGG CCCTGTGGGG AGCAGGGCAG CTGTGTGCCA 
TTGCTGCTTG ATCACGCGGA TATGGTGAAC TGTACCTCTG GTGGCCCGGG TCTCATGAAG 
TGCTCTATCA CCTGTCAGAG GGGGTCTGCC CTTCAGGCCA GCAATGGGCA GTACCTCAGG 
CCCATGCAGA AGAAAATTCT GCTCACATGT TCCTCTGGGC ACTGGGACCG GGCTGTGAGC 
TGCCAACCCC TTGACTGTGG TGTCCCCGAC GCATCTTTGG TGAACTACGC AAATTTCTCC 
TGCTTGGAGG GAACCAACTT TCTGAAACGC TGCTCTATCT CCTGTATCCC ACCAGCCAAG 
CTTCAAGGAC TGAGCCCATG GCTGACCTGT CTTGAAGATG GGCTCTGGTC TCTCCCTGAA 
GCTTACTGCA AGCTGGAGTG TGATGCTCCT CCTGTTATCC CGAATGCCAA CCTGCTCCAG 
CCTCGCTGCC TCCAGGGGAA CCATGACGTG GGCTCTACCT GCAGATATGA ATGCAAACCG 
GGCTACTATG TGCTGGGAAG CACAGAGAAT AAAGTCAGGA ACAAGTCCCT GAAGATCCAG 
TGCCTGGAGA GTGGAATCTG GGAGCAAGGC AGCTGTGTCC CAGTGGTGTG CGAGCCCCCC 
TCCCCTGTCT TTGAAGGCAT GTATGAATGC ACCAATGGCT TTGAGCTCGA CAGCCAGTGT 
TTTATCAACT GTAACCAGGA AAGTAAAAGG CTTCCCATCC GCTGCACGAA GGAGGGACAG 
TGGAGCGAGG AGTTCAAGTT ATGTGAGAAC CTGCGAGGGG AATGCCCACC ACCCACCTCA 
GACCCCAAAT TTGTGGAGTA CAAGTGTGAA CAAGGACACG GGATAGGTGC AGTGTGTTCC 
CCATTGTGTA TCGTCCTCCC TAGTGATCCT GTAATCCTTC CTGAGAATGT CACTACTGAC 
ACTCTGGAGC ACTGGATGGA ACCCGTCAGA GTACAGAGTG TCATGTGTAC TGGACGGCGT 
CAGTGGCACC CAGACCCCCT CCTGATCCAC TGCATCCAGT CCTGCGAGCC TTTCCAAGGG 
GACGGTTGGT GTGACACCAT CAATAACCGG GCCTATTGCA ACTATGATGG GGGAGACTGC 
TGCCCTTCTA CACTCTCCTC CAAGAAGGTC ATTCCATTTG CTGCTGACTG TGATCAGGAT 
GAATGCACCT GCCGAGACCC CAGGGCTGAA GAAAACCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PAPPA2 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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