NAEGRDRAFT_45438 cDNA ORF clone, Naegleria gruberi strain NEG-M

Gene
Clones
gRNAs

Summary

Gene Symbol	NAEGRDRAFT_45438
Entrez Gene ID	8863254
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Naegleria gruberi strain NEG-M

mRNA and Protein(s)

mRNA	Protein	Name
XM_002682619.1	XP_002682665.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of Naegleria gruberi illuminates early eukaryotic versatility.
Fritz-Laylin LK, Prochnik SE, Ginger ML, Dacks JB, Carpenter ML, Field MC, Kuo A, Paredez A, Chapman J, Pham J, Shu S, Neupane R, Cipriano M, Mancuso J, Tu H, Salamov A, Lindquist E, Shapiro H, Lucas S, Grigoriev IV, Cande WZ, Fulton C, Rokhsar DS, Dawson SC
Cell140(5)631-42(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NAEGRDRAFT_45438 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAEGRDRAFT_45438 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONa13944	XM_002682619.1 Latest version!	Naegleria gruberi predicted protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAEGRDRAFT_45438 ( XM_002682619.1 ) cDNA ORF clone, Naegleria gruberi strain NEG-M -> XP_002682665.1 Naegleria gruberi predicted protein, mRNA.

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CloneID	ONa13944
Clone ID Related Accession (Same CDS sequence)	XM_002682619.1
Accession Version	XM_002682619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1269705600000
Organism	Naegleria gruberi strain NEG-M
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003163325). COMPLETENESS: incomplete on both ends.

ATGTTCGCCA CTGGTCCCAC ATCATCTTCA ACAACACGTG AATCATCAGG TCAAAACAAT 
GATAATGATA CCACAAGAGT CTCATCAACA CATATCATTG TTACACCCGA TACAACTTTT 
CCTACTTTCC AACTTTCCAA TACTCTCCAT TCTACAGTCC CACAACAAAC AATTGACAAG 
AGAACAAGTT ATAGCGCACT GGAGGAGAGA AAGAGAATTA GAGCAAAGAG GAAACAGTTT 
AATAATTATC AACAACAAGA AGGACAATCT ACAGAAGAAC CAACATATCC ACCAGTGAAT 
AAGTCGTCTT CATCATCGTA TTATCAAAAT TATAATGACA CAATTCAAAG ACCAATACAA 
CCTCCAACAA GATCTGTGAT TACAGAGGAG GAAATGAACA AGGATTTGGC AACCTTCAAG 
AGGCAAATAA AGAAGGAAAA GAGGGAGAAA CAAAGAGTTT CAAAGAAAAA AGAAAACAAT 
TTTAATCCTC TGGAAAGTAA TATTACCATT GATAATAATT CCGAATCAAA CGATAAAGCT 
TCATCATCAG TAGCTTCTAC TAGTAGTAGT AATGTTACTG CAAGTAACAT CTATGACAGG 
AAACAAAGAA ACAAGAAGGA AAAGAAACCT AATGATTTTA GGAGGGTTAA AAACTACTTC 
CCTCCAGTTC CAGAAGGACA ACAATTGAAT GTTCAAGGCC CTCGTTTAAT TGAATCTCAA 
CCAGAATCTT TAAGTGCCAC AACTAACAAG CCTTCAACAA GAATTAGAGA AAACAAAAGG 
GTAAATAGCC AAGCTATACA AAACAATTCA ATTGAGAGCT CTGTGACTTT ACCTGTCGAA 
AAGTTACCAA AAGCAAAAAC CAAGAAGGAA AACCAGTTAC TTCCTCAAAT AGGTCAAATC 
AAATTGAACA ATGAACAACA TACAAATACT AAACCAAAAG CTATGGATTC ACTTCCTATT 
CATGAATTTT CATCTTCAAT TATCAATTCT ATTGAAGAAA ATCAAGTCAC TGTCATAACA 
GGTTTCACAG AAGGATGCTT GACTCAAATC ATCATGGGTA TGCATTCTTC ATCAACATCA 
AGCTATGAAA ATTTTCATAA TGAATTTTCA CATATAGTAC TAGACGAAAT TCACGAAAGA 
AAAGTCGACA CAGATCTCCT TCTTTATTTT GTGAGAGAAA TTGTCAAGGT TAATCCTAAA 
ATTAGAATCA TTTTGATGAG TGCCACACTC TCACAAGAAA AGATTTGTAA ATATTTTGAG 
AGTTACCTTA ATATGGATAT CTCATCCGCA AAGAACAATG GACAAAATAA TGGGGGAAAT 
GGAGAGGATG ATGAGCTTTT CGTTTCAAAC ATAGAAAAGA AGGTTCCTGC AATTCATATT 
CCTAGTGGAT GTTATCCCGT AACTGAAAAC CACATTGAAG ATGTGATTAC CCTTTTATAC 
AACCCTGTTT CTAATCCTCT CCCAGAGGAA GAATTATTGA GCAAGCAGCA ATTTGCTCCC 
TTGCTTTCTA AACCATTGAG AGCCAGCATA AAACCAGAGA GAAGAATTTT AGGAGCCCGT 
CTTATTGAAT ACCTACACGT ACGTTATCCA TTAAATCAAT CCATACTAGT ATTCCTTCCA 
GGTATGATGG AGATAGAGGA TTTCGCTGAT GATTTGACAG CTAATTTTAT TGGAGGGGCT 
TCAGCAGTTG ACTGTCCATA CGATATTCAT ATTCTTCACT CCCTCATCGC AGAGCAAGAA 
CAAGAAGAAT CCCTTTTACC ACCAAAAGAA GTTTTGCAAA TACTCATGTT CAATGGAGCT 
TACGTTGATG ACCCAACACA ATTCAAAGGT TTACCTAAGA ATTACAAAAC TCCATCAGAA 
GTTTTGCAAA AGTGTCTAGA TCCTCCAAAT CAAAGTTTGT TAGAGCAGGC TTACTATCAA 
CTTGAATCAC TTGGAGCCAT TGTGAATCAT CAATTGGGAA ATTCTCAAAC TGATTCCAAT 
AGTGTTGTGA ATCAATATGA ATTAGATGAA GCTTACTTGG TTACTAGCAT CGGAAAATTC 
TTTTCTTTAA TTCCAACCGA GTTAAATGCA GCCTACCTTT TACTAATTGG TTATTCATTG 
AATTTATTAC CAGAGTGCAT AATTATTGTT TCCATATTGT CCAGATCAGT GCCAATTCTT 
GCCTTCTCAT CTTTAAAGCT TCTAAGCGCA AGAGCTATTT ATTCATTTGA TAATGGATCT 
GAGAGTGATT TAATTGCTGG ATTCAATGCT TATAACCGCT TCAAACAACT TCAAACATTG 
AGGAGAGATC ACAAAATAAC TCCCAGCCAA TTTAATAGTC AACTGGCCAA GGAAATGCTT 
GCTCTGAAGA AGATGATTGA AATTGATTCT TTAGTGTTAC AAATTTCTCA ATCTCTCTAC 
AGAGCCTATG GTATATTTAT TGAAAACCTT ACTCCGCATG CTATCAAACA CTCAAGGCAA 
GAAATTAAAC CTATTCAATA CTCAACTGAT AAGGTGCTCA GCACTGAAAA GATGAATATT 
ATTAAGGCAC TCATTGCCAC ATTGTACTAT GAACAATCAG TGAAGGGAGC TCTCAAGAAG 
GAACACAAGT CACTCAAAGA AATTGAATGC AGCTTGCAAA TCAAAGATCA AGCCCTTTCA 
CTCACAAATA GTGATGAGAT GCAGAAAGTT TTCTCAGAAC TTTTCCATAA ATTTGACGGA 
GGTGTTGTTG ATTGTACAAA GATCAAGTTC CAATCCGATA AGAAGATTAA TCTCGCCTAT 
AACCTTCCTG TATCTGATTC ATCATTTGTG TTGCCACTTA CAGAAGCTCA AAAGAAGGAC 
CCACTTTTCC CAATTAAATT GCTACAACGT ATGATCAAAG GTAGTTCACA AGAATCAAAA 
GAAAAGAAAA ATCAAACACT TGATTATACA CAGGTGAGAC AAAGTTTGCT TTCAGTGAGG 
GATGATGACC AAATTTTTGG TATTTTCAGA GGACTTCCAG GTGAATTGAT CAAGTCCCAT 
GGATTGAAAT TCAAAGCAAT GTCTTCAGCA TTTGATAGAA AGATTTCATT TATTCATCCA 
CAATCAGATG CAATGAGAAA ACAAAGAATA ATCATTTCTA GAGATTCTGT TTCTCACCCT 
TGGGTTGATT TAACAATACC AGAAAAAATT CTCGTCTCTG GAAGTGTATC AATCAAGGAT 
ATGACCATGT TACTTGATAA GAATACGACA CTTCCTGACA TTGAATTTTT TTATGAAATG 
TCCTTAGTAT TATTCTTGCC TTTGATTTAT CCAAGAAGAT TTTGCCTTTT CAAACCAAAG 
TATGGCGATC AAACATTTTG CCTCATATCA GAACTCTTTG AAAAGATGAA ACCACATGAG 
AAGACTGAAT ACGTTCAAAA TAAGATTAAT CTTGATTTGA ATGCTATCTA TTTAATTTCT 
AGTGGTGTTA AAAACTTTGA CTTGGTTTTA CAAATTCTGA GAAGTTCCAT CCAAAAGAAG 
ATTACTGAAT TACTAGCCAA TCAACCAGTT GTTGATGTTG GTCTGTTATC CGTTCAAATA 
ACTGATCTAC TCAATAATGC CAAGAATTAC CTTTCTCACA AAGGTTACAA TAGAAACATC 
GCCAAAATTA TCTCCGTCTT ACCTGCAGAA GATGAAGCTG TGGGAGGAGA TGATGAATCA 
TGTGGTGATC ACATTCTTAG AACTAGTAGT TTCCAAAGAA TTTCTCAAAG AATATTGAAC 
CTATTGTCTA AACAGTGTCT TCTTTCTCCT GATGATCCAC CTGTCGAAAT CAAAAAGAAG 
GGTAAAACAA CTATAGAAAT AAGCTGTTCA TATGATTATC AATACCTACA AGCCAAATAT 
CTCTTGTTGC GAGAGATTAA CAATGATTAT GCTGTTTTTG CACATCCAAA ACAAGCATTT 
GTATGCTTGG AAGTTGAGAA GAATAATTTG AAAGACTTTT TATCATCAAT GGTATACTTT 
TCAAGTTATG AGGATAAATC TGGAAAGAAT AGAGAATTCT ATACAATCAA TAGAATTCCA 
GCAACCAAAC GGTTAAATAT TAGCATAGAT GAAGGTAAAG AAGGTAATAA TGTAAGAAAA 
TCATTCAATA CCAAGATGAA ATCTGACAAG CTTACTAATG GAGGATTTCT TCTTCAACCT 
TTTACACGCT CGATCTCAGC AGATGCTTTC AGAGAAAAAA TTCCAGCCAA TAGCAGCAAT 
GATGAATACA TGCAAGTGTA TGCATACTTT GGGAAACAAG TTTTTTATCA AGCAAAGGCA 
GGAGCAACAA AGATTCACTC TCAACAAAAG GGAGTAACAT TTGAGTCACT TAGAAAGATG 
AAGATTGGTA ACGATAATGA TGTCAAAACT CTATTCTTAC ACTTTATAGA TGCAAGACGT 
CAGCCTGATT CTTCTCTAAT TTCAAACTTC TTACTTTCCA AGAAAGCAGA AAATAAGGAA 
AGTGTATTCT CCAACAATCA TTACTTTAAG AGAATTCTCG TTGATAAGAA TCCAGTTTTG 
GGAAAGCAAA AGGAGTACAT TTCACTCAAT ATTGTAGACA TGGACAAGAA GGAGAGATTT 
ACAATCTTGC TCTACCTCGA CAGAGAACAG ACCTACAAGG ATGAAAATAC TGACTTGGTT 
AAATGTTCTA TGCAAAGCTG GAGAAGATCT GATACCAAAC TGATTTATTT AGATTTTGTC 
TCTCCAATAC TTGTACAACC AAATGATAAT GCTGAAACAA TATCGAATAA GAATGAAAAT 
ACATTGGACG TGAGATTTTC CAAAGTTACT CACTCTAGAA CTATCAAGGA AGGGTTTAGA 
GATTTAATTC CAGCATCCAT CCAAGAGTTG GAGTCAAGAT TGTGGTACAA TGAGAGCGAC 
TTTACACTTT ATTATTGTTA TGAGAAGGAT GATTCTATAT TATTTGCCAA TCTGAATGAT 
TTGAATTTGT CATTTGGCAG TACATTGGAA AATGATGAGT TGTGCAAATT GAGAGCTGAA 
ACACCACTTG CACGTCTCTC AATCGATTCT ATTCACTACG ATATCAAGGA AAATTTGCAC 
TTTAACGATA TTAAGATTCA ATTTAAGAAT AGAACCGACA TTTCACATCC AACCCAAAGA 
AAATCTTTCA CTACCCTTAG GGTTTGTAGC CATCAATTAC AAGGAGCTTT AGCAACGAGT 
AATAATATCT ATGTCTTGTT AGATTCCGAT GATCATCAGC AACAATTATC AACCTCTCCT 
ACTTCAATAA TTAATATATT AAATAATTCT AGCAATAATA CTACAATTAA CAGTAATATT 
GCAAATAATA ATAATAAGGA TGTATTTATT CAACGTGAAT TTCCCATTCA AGAAATTCCA 
ATTTATAATA TTAGTAGAAT TCCAGCAGAC TCTCAGATAA TTTCCATCTC AGCCTTTAAT 
TCTAATTATG ATAAGAGTAA GCTAGTGTTG GGAGTTACAT TCACACAACC AATTAATACT 
AGTATTAATA CATCATTGGA TAATAGTGAA CAAAAGAGAA AGTATATTGA TGATGATGAA 
AATAGAGATG AGGATCAATA TTCGTCATCG TATGATTCTG GAGGTGGAGG TGATAGTACA 
GATGAGGATT CGGAAATGAA TACTATTCAT TCAAAAAAGA AAAAGAAGAA GGTTAATACA 
AGTGGAGGTG GCTCTTCATT TGTGTCACAT TCAAAGATGA ATGAAAATGA AGAGAATGAA 
CAACAGAATC AACAGAATCA ACAATTTAAT AATTATTTCT ACATTTACTT GTTGGATATT 
GATGAAATTG TGAATAATCA TAATAATATC AAGTACAATT CATTATTGTA CAGTAGTAAT 
ATTAATACAC AAAATCAAAC CAAGCACAAG TGTATTCAAA CATTCTCTTT AGGATATGTT 
CCATTGAAGT TGAGCACTCA AACAACAACT CTATATCCCA ATTTACAAAA ATACTCAATA 
TTTGTCATTT GTGGAAGTGA TAAGAATTTT CATTGTTTTT CTGATATTAT TCTCACTCCT 
CAAAATAATT CAATCCTTCT CAAATTATTT GACCTTCCTC TCTACACTAG GAATAATGAA 
TATGCCAGTA ATATTAGACA TTATTACAAT AGATTCAATG AAAATTTATT TATTGAATAT 
GATTTTATTA TACCAGGAGG TGTACCAGGT TCTGTCATTG ATTTTGATTC TAATAATAAC 
TTATTGGTCA TGGGATGTCA AAATGGTTTT GTGAAAATTT GTAAAGGTGC CAATCTTGAA 
CTACAGTTCA TGTTGAATAG TCCAATTAGT TCTGTAAAAA TTTTAAACTA TACACCTACA 
GAGTTGAGTT TGTTGGTGGG TGAATCTGTT GGTAGGGTTG TCCTCTACAA TGTTCCCCTC 
ACTTCCTTTG TCAAGGATAA TAATAATCAA ATGGTTCCCT CCACTCCCTC CATAATCAAT 
AATACCAATG AAATAATGAC CAATATCCAA TCTACTGTTA TTTACAGTGA CGAGTTTGGA 
GACTCGATTA CATGTCTCTA TGCCCAACCT TACAGGTGTG GCATTACGGA TATTTATATT 
GGTACCTACA ATAGAAAACT AATTGTCTAC CAATTTGAGA GTAATACATG CATTTACAAA 
TGTACCTATC ATTTTGAATA TCCAATACAA TACATTGATT CATTGGACAT TAATAATGAT 
GGAGTGAAGG AAATTATCAT TTGCACCATG TTTGAAATTC AAATATTACA ACCTGACCTT 
ACCTCTATTG CCAATTATAT CAAGGAAAAG TTAAAACTCA ATAATGCAAC ATTGCCAACA 
GAGACAACTA ACCACCACCA ATCCATAACC ACTACCTTAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002682665.1
CDS	1..6825
Translation	MFATGPTSSSTTRESSGQNNDNDTTRVSSTHIIVTPDTTFPTFQLSNTLHSTVPQQTIDKRTSYSALEERKRIRAKRKQFNNYQQQEGQSTEEPTYPPVNKSSSSSYYQNYNDTIQRPIQPPTRSVITEEEMNKDLATFKRQIKKEKREKQRVSKKKENNFNPLESNITIDNNSESNDKASSSVASTSSSNVTASNIYDRKQRNKKEKKPNDFRRVKNYFPPVPEGQQLNVQGPRLIESQPESLSATTNKPSTRIRENKRVNSQAIQNNSIESSVTLPVEKLPKAKTKKENQLLPQIGQIKLNNEQHTNTKPKAMDSLPIHEFSSSIINSIEENQVTVITGFTEGCLTQIIMGMHSSSTSSYENFHNEFSHIVLDEIHERKVDTDLLLYFVREIVKVNPKIRIILMSATLSQEKICKYFESYLNMDISSAKNNGQNNGGNGEDDELFVSNIEKKVPAIHIPSGCYPVTENHIEDVITLLYNPVSNPLPEEELLSKQQFAPLLSKPLRASIKPERRILGARLIEYLHVRYPLNQSILVFLPGMMEIEDFADDLTANFIGGASAVDCPYDIHILHSLIAEQEQEESLLPPKEVLQILMFNGAYVDDPTQFKGLPKNYKTPSEVLQKCLDPPNQSLLEQAYYQLESLGAIVNHQLGNSQTDSNSVVNQYELDEAYLVTSIGKFFSLIPTELNAAYLLLIGYSLNLLPECIIIVSILSRSVPILAFSSLKLLSARAIYSFDNGSESDLIAGFNAYNRFKQLQTLRRDHKITPSQFNSQLAKEMLALKKMIEIDSLVLQISQSLYRAYGIFIENLTPHAIKHSRQEIKPIQYSTDKVLSTEKMNIIKALIATLYYEQSVKGALKKEHKSLKEIECSLQIKDQALSLTNSDEMQKVFSELFHKFDGGVVDCTKIKFQSDKKINLAYNLPVSDSSFVLPLTEAQKKDPLFPIKLLQRMIKGSSQESKEKKNQTLDYTQVRQSLLSVRDDDQIFGIFRGLPGELIKSHGLKFKAMSSAFDRKISFIHPQSDAMRKQRIIISRDSVSHPWVDLTIPEKILVSGSVSIKDMTMLLDKNTTLPDIEFFYEMSLVLFLPLIYPRRFCLFKPKYGDQTFCLISELFEKMKPHEKTEYVQNKINLDLNAIYLISSGVKNFDLVLQILRSSIQKKITELLANQPVVDVGLLSVQITDLLNNAKNYLSHKGYNRNIAKIISVLPAEDEAVGGDDESCGDHILRTSSFQRISQRILNLLSKQCLLSPDDPPVEIKKKGKTTIEISCSYDYQYLQAKYLLLREINNDYAVFAHPKQAFVCLEVEKNNLKDFLSSMVYFSSYEDKSGKNREFYTINRIPATKRLNISIDEGKEGNNVRKSFNTKMKSDKLTNGGFLLQPFTRSISADAFREKIPANSSNDEYMQVYAYFGKQVFYQAKAGATKIHSQQKGVTFESLRKMKIGNDNDVKTLFLHFIDARRQPDSSLISNFLLSKKAENKESVFSNNHYFKRILVDKNPVLGKQKEYISLNIVDMDKKERFTILLYLDREQTYKDENTDLVKCSMQSWRRSDTKLIYLDFVSPILVQPNDNAETISNKNENTLDVRFSKVTHSRTIKEGFRDLIPASIQELESRLWYNESDFTLYYCYEKDDSILFANLNDLNLSFGSTLENDELCKLRAETPLARLSIDSIHYDIKENLHFNDIKIQFKNRTDISHPTQRKSFTTLRVCSHQLQGALATSNNIYVLLDSDDHQQQLSTSPTSIINILNNSSNNTTINSNIANNNNKDVFIQREFPIQEIPIYNISRIPADSQIISISAFNSNYDKSKLVLGVTFTQPINTSINTSLDNSEQKRKYIDDDENRDEDQYSSSYDSGGGGDSTDEDSEMNTIHSKKKKKKVNTSGGGSSFVSHSKMNENEENEQQNQQNQQFNNYFYIYLLDIDEIVNNHNNIKYNSLLYSSNINTQNQTKHKCIQTFSLGYVPLKLSTQTTTLYPNLQKYSIFVICGSDKNFHCFSDIILTPQNNSILLKLFDLPLYTRNNEYASNIRHYYNRFNENLFIEYDFIIPGGVPGSVIDFDSNNNLLVMGCQNGFVKICKGANLELQFMLNSPISSVKILNYTPTELSLLVGESVGRVVLYNVPLTSFVKDNNNQMVPSTPSIINNTNEIMTNIQSTVIYSDEFGDSITCLYAQPYRCGITDIYIGTYNRKLIVYQFESNTCIYKCTYHFEYPIQYIDSLDINNDGVKEIIICTMFEIQILQPDLTSIANYIKEKLKLNNATLPTETTNHHQSITTTLE

Target ORF information:

RefSeq Version	XM_002682619.1
Organism	Naegleria gruberi strain NEG-M
Definition	Naegleria gruberi predicted protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002682619.1

ORF Insert Sequence:

ATGTTCGCCA CTGGTCCCAC ATCATCTTCA ACAACACGTG AATCATCAGG TCAAAACAAT 
GATAATGATA CCACAAGAGT CTCATCAACA CATATCATTG TTACACCCGA TACAACTTTT 
CCTACTTTCC AACTTTCCAA TACTCTCCAT TCTACAGTCC CACAACAAAC AATTGACAAG 
AGAACAAGTT ATAGCGCACT GGAGGAGAGA AAGAGAATTA GAGCAAAGAG GAAACAGTTT 
AATAATTATC AACAACAAGA AGGACAATCT ACAGAAGAAC CAACATATCC ACCAGTGAAT 
AAGTCGTCTT CATCATCGTA TTATCAAAAT TATAATGACA CAATTCAAAG ACCAATACAA 
CCTCCAACAA GATCTGTGAT TACAGAGGAG GAAATGAACA AGGATTTGGC AACCTTCAAG 
AGGCAAATAA AGAAGGAAAA GAGGGAGAAA CAAAGAGTTT CAAAGAAAAA AGAAAACAAT 
TTTAATCCTC TGGAAAGTAA TATTACCATT GATAATAATT CCGAATCAAA CGATAAAGCT 
TCATCATCAG TAGCTTCTAC TAGTAGTAGT AATGTTACTG CAAGTAACAT CTATGACAGG 
AAACAAAGAA ACAAGAAGGA AAAGAAACCT AATGATTTTA GGAGGGTTAA AAACTACTTC 
CCTCCAGTTC CAGAAGGACA ACAATTGAAT GTTCAAGGCC CTCGTTTAAT TGAATCTCAA 
CCAGAATCTT TAAGTGCCAC AACTAACAAG CCTTCAACAA GAATTAGAGA AAACAAAAGG 
GTAAATAGCC AAGCTATACA AAACAATTCA ATTGAGAGCT CTGTGACTTT ACCTGTCGAA 
AAGTTACCAA AAGCAAAAAC CAAGAAGGAA AACCAGTTAC TTCCTCAAAT AGGTCAAATC 
AAATTGAACA ATGAACAACA TACAAATACT AAACCAAAAG CTATGGATTC ACTTCCTATT 
CATGAATTTT CATCTTCAAT TATCAATTCT ATTGAAGAAA ATCAAGTCAC TGTCATAACA 
GGTTTCACAG AAGGATGCTT GACTCAAATC ATCATGGGTA TGCATTCTTC ATCAACATCA 
AGCTATGAAA ATTTTCATAA TGAATTTTCA CATATAGTAC TAGACGAAAT TCACGAAAGA 
AAAGTCGACA CAGATCTCCT TCTTTATTTT GTGAGAGAAA TTGTCAAGGT TAATCCTAAA 
ATTAGAATCA TTTTGATGAG TGCCACACTC TCACAAGAAA AGATTTGTAA ATATTTTGAG 
AGTTACCTTA ATATGGATAT CTCATCCGCA AAGAACAATG GACAAAATAA TGGGGGAAAT 
GGAGAGGATG ATGAGCTTTT CGTTTCAAAC ATAGAAAAGA AGGTTCCTGC AATTCATATT 
CCTAGTGGAT GTTATCCCGT AACTGAAAAC CACATTGAAG ATGTGATTAC CCTTTTATAC 
AACCCTGTTT CTAATCCTCT CCCAGAGGAA GAATTATTGA GCAAGCAGCA ATTTGCTCCC 
TTGCTTTCTA AACCATTGAG AGCCAGCATA AAACCAGAGA GAAGAATTTT AGGAGCCCGT 
CTTATTGAAT ACCTACACGT ACGTTATCCA TTAAATCAAT CCATACTAGT ATTCCTTCCA 
GGTATGATGG AGATAGAGGA TTTCGCTGAT GATTTGACAG CTAATTTTAT TGGAGGGGCT 
TCAGCAGTTG ACTGTCCATA CGATATTCAT ATTCTTCACT CCCTCATCGC AGAGCAAGAA 
CAAGAAGAAT CCCTTTTACC ACCAAAAGAA GTTTTGCAAA TACTCATGTT CAATGGAGCT 
TACGTTGATG ACCCAACACA ATTCAAAGGT TTACCTAAGA ATTACAAAAC TCCATCAGAA 
GTTTTGCAAA AGTGTCTAGA TCCTCCAAAT CAAAGTTTGT TAGAGCAGGC TTACTATCAA 
CTTGAATCAC TTGGAGCCAT TGTGAATCAT CAATTGGGAA ATTCTCAAAC TGATTCCAAT 
AGTGTTGTGA ATCAATATGA ATTAGATGAA GCTTACTTGG TTACTAGCAT CGGAAAATTC 
TTTTCTTTAA TTCCAACCGA GTTAAATGCA GCCTACCTTT TACTAATTGG TTATTCATTG 
AATTTATTAC CAGAGTGCAT AATTATTGTT TCCATATTGT CCAGATCAGT GCCAATTCTT 
GCCTTCTCAT CTTTAAAGCT TCTAAGCGCA AGAGCTATTT ATTCATTTGA TAATGGATCT 
GAGAGTGATT TAATTGCTGG ATTCAATGCT TATAACCGCT TCAAACAACT TCAAACATTG 
AGGAGAGATC ACAAAATAAC TCCCAGCCAA TTTAATAGTC AACTGGCCAA GGAAATGCTT 
GCTCTGAAGA AGATGATTGA AATTGATTCT TTAGTGTTAC AAATTTCTCA ATCTCTCTAC 
AGAGCCTATG GTATATTTAT TGAAAACCTT ACTCCGCATG CTATCAAACA CTCAAGGCAA 
GAAATTAAAC CTATTCAATA CTCAACTGAT AAGGTGCTCA GCACTGAAAA GATGAATATT 
ATTAAGGCAC TCATTGCCAC ATTGTACTAT GAACAATCAG TGAAGGGAGC TCTCAAGAAG 
GAACACAAGT CACTCAAAGA AATTGAATGC AGCTTGCAAA TCAAAGATCA AGCCCTTTCA 
CTCACAAATA GTGATGAGAT GCAGAAAGTT TTCTCAGAAC TTTTCCATAA ATTTGACGGA 
GGTGTTGTTG ATTGTACAAA GATCAAGTTC CAATCCGATA AGAAGATTAA TCTCGCCTAT 
AACCTTCCTG TATCTGATTC ATCATTTGTG TTGCCACTTA CAGAAGCTCA AAAGAAGGAC 
CCACTTTTCC CAATTAAATT GCTACAACGT ATGATCAAAG GTAGTTCACA AGAATCAAAA 
GAAAAGAAAA ATCAAACACT TGATTATACA CAGGTGAGAC AAAGTTTGCT TTCAGTGAGG 
GATGATGACC AAATTTTTGG TATTTTCAGA GGACTTCCAG GTGAATTGAT CAAGTCCCAT 
GGATTGAAAT TCAAAGCAAT GTCTTCAGCA TTTGATAGAA AGATTTCATT TATTCATCCA 
CAATCAGATG CAATGAGAAA ACAAAGAATA ATCATTTCTA GAGATTCTGT TTCTCACCCT 
TGGGTTGATT TAACAATACC AGAAAAAATT CTCGTCTCTG GAAGTGTATC AATCAAGGAT 
ATGACCATGT TACTTGATAA GAATACGACA CTTCCTGACA TTGAATTTTT TTATGAAATG 
TCCTTAGTAT TATTCTTGCC TTTGATTTAT CCAAGAAGAT TTTGCCTTTT CAAACCAAAG 
TATGGCGATC AAACATTTTG CCTCATATCA GAACTCTTTG AAAAGATGAA ACCACATGAG 
AAGACTGAAT ACGTTCAAAA TAAGATTAAT CTTGATTTGA ATGCTATCTA TTTAATTTCT 
AGTGGTGTTA AAAACTTTGA CTTGGTTTTA CAAATTCTGA GAAGTTCCAT CCAAAAGAAG 
ATTACTGAAT TACTAGCCAA TCAACCAGTT GTTGATGTTG GTCTGTTATC CGTTCAAATA 
ACTGATCTAC TCAATAATGC CAAGAATTAC CTTTCTCACA AAGGTTACAA TAGAAACATC 
GCCAAAATTA TCTCCGTCTT ACCTGCAGAA GATGAAGCTG TGGGAGGAGA TGATGAATCA 
TGTGGTGATC ACATTCTTAG AACTAGTAGT TTCCAAAGAA TTTCTCAAAG AATATTGAAC 
CTATTGTCTA AACAGTGTCT TCTTTCTCCT GATGATCCAC CTGTCGAAAT CAAAAAGAAG 
GGTAAAACAA CTATAGAAAT AAGCTGTTCA TATGATTATC AATACCTACA AGCCAAATAT 
CTCTTGTTGC GAGAGATTAA CAATGATTAT GCTGTTTTTG CACATCCAAA ACAAGCATTT 
GTATGCTTGG AAGTTGAGAA GAATAATTTG AAAGACTTTT TATCATCAAT GGTATACTTT 
TCAAGTTATG AGGATAAATC TGGAAAGAAT AGAGAATTCT ATACAATCAA TAGAATTCCA 
GCAACCAAAC GGTTAAATAT TAGCATAGAT GAAGGTAAAG AAGGTAATAA TGTAAGAAAA 
TCATTCAATA CCAAGATGAA ATCTGACAAG CTTACTAATG GAGGATTTCT TCTTCAACCT 
TTTACACGCT CGATCTCAGC AGATGCTTTC AGAGAAAAAA TTCCAGCCAA TAGCAGCAAT 
GATGAATACA TGCAAGTGTA TGCATACTTT GGGAAACAAG TTTTTTATCA AGCAAAGGCA 
GGAGCAACAA AGATTCACTC TCAACAAAAG GGAGTAACAT TTGAGTCACT TAGAAAGATG 
AAGATTGGTA ACGATAATGA TGTCAAAACT CTATTCTTAC ACTTTATAGA TGCAAGACGT 
CAGCCTGATT CTTCTCTAAT TTCAAACTTC TTACTTTCCA AGAAAGCAGA AAATAAGGAA 
AGTGTATTCT CCAACAATCA TTACTTTAAG AGAATTCTCG TTGATAAGAA TCCAGTTTTG 
GGAAAGCAAA AGGAGTACAT TTCACTCAAT ATTGTAGACA TGGACAAGAA GGAGAGATTT 
ACAATCTTGC TCTACCTCGA CAGAGAACAG ACCTACAAGG ATGAAAATAC TGACTTGGTT 
AAATGTTCTA TGCAAAGCTG GAGAAGATCT GATACCAAAC TGATTTATTT AGATTTTGTC 
TCTCCAATAC TTGTACAACC AAATGATAAT GCTGAAACAA TATCGAATAA GAATGAAAAT 
ACATTGGACG TGAGATTTTC CAAAGTTACT CACTCTAGAA CTATCAAGGA AGGGTTTAGA 
GATTTAATTC CAGCATCCAT CCAAGAGTTG GAGTCAAGAT TGTGGTACAA TGAGAGCGAC 
TTTACACTTT ATTATTGTTA TGAGAAGGAT GATTCTATAT TATTTGCCAA TCTGAATGAT 
TTGAATTTGT CATTTGGCAG TACATTGGAA AATGATGAGT TGTGCAAATT GAGAGCTGAA 
ACACCACTTG CACGTCTCTC AATCGATTCT ATTCACTACG ATATCAAGGA AAATTTGCAC 
TTTAACGATA TTAAGATTCA ATTTAAGAAT AGAACCGACA TTTCACATCC AACCCAAAGA 
AAATCTTTCA CTACCCTTAG GGTTTGTAGC CATCAATTAC AAGGAGCTTT AGCAACGAGT 
AATAATATCT ATGTCTTGTT AGATTCCGAT GATCATCAGC AACAATTATC AACCTCTCCT 
ACTTCAATAA TTAATATATT AAATAATTCT AGCAATAATA CTACAATTAA CAGTAATATT 
GCAAATAATA ATAATAAGGA TGTATTTATT CAACGTGAAT TTCCCATTCA AGAAATTCCA 
ATTTATAATA TTAGTAGAAT TCCAGCAGAC TCTCAGATAA TTTCCATCTC AGCCTTTAAT 
TCTAATTATG ATAAGAGTAA GCTAGTGTTG GGAGTTACAT TCACACAACC AATTAATACT 
AGTATTAATA CATCATTGGA TAATAGTGAA CAAAAGAGAA AGTATATTGA TGATGATGAA 
AATAGAGATG AGGATCAATA TTCGTCATCG TATGATTCTG GAGGTGGAGG TGATAGTACA 
GATGAGGATT CGGAAATGAA TACTATTCAT TCAAAAAAGA AAAAGAAGAA GGTTAATACA 
AGTGGAGGTG GCTCTTCATT TGTGTCACAT TCAAAGATGA ATGAAAATGA AGAGAATGAA 
CAACAGAATC AACAGAATCA ACAATTTAAT AATTATTTCT ACATTTACTT GTTGGATATT 
GATGAAATTG TGAATAATCA TAATAATATC AAGTACAATT CATTATTGTA CAGTAGTAAT 
ATTAATACAC AAAATCAAAC CAAGCACAAG TGTATTCAAA CATTCTCTTT AGGATATGTT 
CCATTGAAGT TGAGCACTCA AACAACAACT CTATATCCCA ATTTACAAAA ATACTCAATA 
TTTGTCATTT GTGGAAGTGA TAAGAATTTT CATTGTTTTT CTGATATTAT TCTCACTCCT 
CAAAATAATT CAATCCTTCT CAAATTATTT GACCTTCCTC TCTACACTAG GAATAATGAA 
TATGCCAGTA ATATTAGACA TTATTACAAT AGATTCAATG AAAATTTATT TATTGAATAT 
GATTTTATTA TACCAGGAGG TGTACCAGGT TCTGTCATTG ATTTTGATTC TAATAATAAC 
TTATTGGTCA TGGGATGTCA AAATGGTTTT GTGAAAATTT GTAAAGGTGC CAATCTTGAA 
CTACAGTTCA TGTTGAATAG TCCAATTAGT TCTGTAAAAA TTTTAAACTA TACACCTACA 
GAGTTGAGTT TGTTGGTGGG TGAATCTGTT GGTAGGGTTG TCCTCTACAA TGTTCCCCTC 
ACTTCCTTTG TCAAGGATAA TAATAATCAA ATGGTTCCCT CCACTCCCTC CATAATCAAT 
AATACCAATG AAATAATGAC CAATATCCAA TCTACTGTTA TTTACAGTGA CGAGTTTGGA 
GACTCGATTA CATGTCTCTA TGCCCAACCT TACAGGTGTG GCATTACGGA TATTTATATT 
GGTACCTACA ATAGAAAACT AATTGTCTAC CAATTTGAGA GTAATACATG CATTTACAAA 
TGTACCTATC ATTTTGAATA TCCAATACAA TACATTGATT CATTGGACAT TAATAATGAT 
GGAGTGAAGG AAATTATCAT TTGCACCATG TTTGAAATTC AAATATTACA ACCTGACCTT 
ACCTCTATTG CCAATTATAT CAAGGAAAAG TTAAAACTCA ATAATGCAAC ATTGCCAACA 
GAGACAACTA ACCACCACCA ATCCATAACC ACTACCTTAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of Naegleria gruberi illuminates early eukaryotic versatility.
Cell140(5)631-42(2010 Mar)
Fritz-Laylin LK,Prochnik SE,Ginger ML,Dacks JB,Carpenter ML,Field MC,Kuo A,Paredez A,Chapman J,Pham J,Shu S,Neupane R,Cipriano M,Mancuso J,Tu H,Salamov A,Lindquist E,Shapiro H,Lucas S,Grigoriev IV,Cande WZ,Fulton C,Rokhsar DS,Dawson SC

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NAEGRDRAFT_45438 cDNA ORF clone, Naegleria gruberi strain NEG-M

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Target ORF information:

Target ORF information:

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