FM142 cDNA ORF clone, Naegleria gruberi strain NEG-M

Gene
Clones
gRNAs

Summary

Gene Symbol	FM142
Entrez Gene ID	8853928
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Naegleria gruberi strain NEG-M

mRNA and Protein(s)

mRNA	Protein	Name
XM_002671247.1	XP_002671293.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of Naegleria gruberi illuminates early eukaryotic versatility.
Fritz-Laylin LK, Prochnik SE, Ginger ML, Dacks JB, Carpenter ML, Field MC, Kuo A, Paredez A, Chapman J, Pham J, Shu S, Neupane R, Cipriano M, Mancuso J, Tu H, Salamov A, Lindquist E, Shapiro H, Lucas S, Grigoriev IV, Cande WZ, Fulton C, Rokhsar DS, Dawson SC
Cell140(5)631-42(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FM142 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FM142 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONa05640	XM_002671247.1 Latest version!	Naegleria gruberi hypothetical protein (FM142), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FM142 ( XM_002671247.1 ) cDNA ORF clone, Naegleria gruberi strain NEG-M -> XP_002671293.1 Naegleria gruberi hypothetical protein (FM142), mRNA.

Price & Availability↑

CloneID	ONa05640
Clone ID Related Accession (Same CDS sequence)	XM_002671247.1
Accession Version	XM_002671247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1269705600000
Organism	Naegleria gruberi strain NEG-M
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003163265). COMPLETENESS: incomplete on both ends.

ATGGCTGAAG AAGATTTTAT ACAATCACTT TCTGTTGGAA TTAGAAGAGC AAAATCAGAT 
GAAGCACCCA ACATACAAAA ATATATTAGT GAGAACAGCT TTCTTCTAAC AAAACGTTTC 
GGAAATTACA ATCTAAAAAC AATAATTGAG ACTGGCTACA TTTCTATTGT GGCTGAAGGT 
GATCAAGGAC AATATCAAGG ATTTGCATCA TTCGAATTTG AGCCATCAGA TACATTAGAT 
GTGGAGAAGT ACTTTTCCTA TCTTCCTCTG AATGTTCGGA ATACAATTTG GATTAGACTT 
GTTCATTTTG AACCCCTCTT TGGAGAGACA GTTTTAATTG AAATGCTAAA TTCAATTTTT 
ATTACTTTCT CTGAAGTTGA CAATATTTGT TTTCAACCAG TAAGATCAAA CGTTCAAAGT 
GGTATTGATT TCTTCTCTCA ATTCTCCTTC TTCAAACAAG AAATTGGATC AGATGTCCTT 
GTCAAGTCTG ACACTGAAAA ATGTATTTTT GTTTGCCACA GAGACAATCT AGGTCGTGGA 
GTTAAGCGTT TGTCAATAAG ACAAGCAGTT GTTGAGGATA ATGACGATAT AATTCCTCTA 
TTATCAAGTT GTGAGAATGT AACCTCCTAC AAAACAGAGG GCGAGTACTT TTTGGCTAAT 
CTCTTGTTTG AACAAAATGA TTCTAAAAAG ATTCTTCTAG CTACTGATAC AGAGAGTGGA 
GACATAGTAG GTGTCATGTA CATTACAACT GAGTTGGATA TTAGGCAAAT TCTCTCGGAT 
TACAAGACTT ATAATGAAGG AGAACCAATA TATGAGGATT TTATAGTTGA AAAACAAATT 
GGTGACAATA TGGTCTACCA TTCAAATGCC TTTGAAATTA AGCTCTTCTA CATTCAAGAG 
GAGTACAAGT TTAGAAGTAT TGATTTATTG AGAGCTGCAT TCAAGGCATT CCAAGAATCT 
AAATATTGCA TTGTGGGAAT CAAATTTGAG GACCATTCCA AATCTGAACA TCCTCTTTTA 
AAACAATTCA TCTATATTGA ACCAACTCAA GAATGTTCTA GCCATTCACT TCATATTCTT 
TCTCAAGTAT CCATGGTTGC AGACTTTTTC GTTGTAAAGG CCACAGAAAC AACACTGGAC 
AAAAATTCAT CACCACAAGA GACTATTCTG AGCTTATTGA GACAGGCAGA ATTTCATGAA 
GAGTTTATTG GTGCAGTAGC CAAAAGTTTT AAAGGAACAA GATATGTAAC CATGTTACTG 
TTTGATAGTA ACTCAGCAGT GGAAGGAAGA AATGAAATTA TTGGTATTGC AGTGGTGGAC 
ACCCAAGTTA ATGAATTTCT GCTTCGTAGA CACTTTGATT TTACAGAATA CATTGATTAT 
TCAGCATATA GCTCAAGAGG AACATGTCAA GCAATGATAA CACATTTTTA TATTGAGCAA 
AAGTTTTACA GATACTCCAA ATTGTTTTTG AAGGAAGTTA TGAGAAAATT AGAAAAACAT 
ATTCTCTACT ATGGCTGCTA CAGAGGTGAA ATTCCAAACG ATAGTCTTAA CAAGGAACTC 
ATTTACTTGA AATCGACCAG ACAAATAGAG TTTCCAGGAG CCTTAAATGA GAGTAGTAGT 
GGAAGAAATT CTTCATTATC TGACAATGAT TCCGTTATCT CTGGAGCTAC TGTTTCTAGC 
GGAGGTGGAT CGAGAGTTTT AACGAATGTA CAATACGATT CTCTCTACTT TATTAATAGA 
AGATATTTGA GTGAAGAAAA AACTGAAATA TTTTCCAGAA TTGTGGTTAT AGGTGGTTCA 
AAAACTGCAC TGAGTTTTAT TAAGGAACTT TCTGCCAACT CTTCTCTTTT CTTTAACAAT 
ATTATTGTCG TATCTCCTGA AGGTGATGTT ACCAAGAGCC CTGAATTTTA TGCCGATGAT 
ACGGATGAAG AATTCAACAA CAGAGAATTG CTTAATATGA TCTCTCTACC AAACATTTAC 
TTTGTGAAAG ATAAACTTGA AGATATAGAA AGAGTCAACA AGAGTGTCCT CTTATACGAA 
AATCAATCCA GCTTCATTAC GTATGATATT TTATTATTAT GTACAAGCAG ACAGTACAAG 
ATGAAACCAA AATATTTAGA ACTGGTTGGA TATCCCAAGA AAGGCATTCT CAATTTGAAC 
CCAAGAGTAC CTGTAAATGA AACAGGAGAA GAAATTGATC TAACCGAATA TTTTGAAATG 
TATTTACATG AAAATGATAG TGAAGAAAGA AGTGATAGTT CTTATATTGT AGTATATGGT 
TCCTCCTTAG ATGCATTGTG TACCGTTAAT ACTTTATTGA ACAGATTTAA TGTTGAGCCA 
TCAAAATTAT TACTCATTTT CCCTGATGGA AAGAAATGTA TTTTTAACGA GAATGAAGAA 
ATTGAAGATA GATTGGACCA TATTTTAGAA GTGGCCGGCC TAAAATACTT TTCTCAATAT 
ACATTGGAAG ATTTTGCCTC TGATGAAGAG GGAAATCTAG TGAGAATTTA CCTCTCACCA 
AATGAGGAAA AGGAAGAGAA GAAAAAACAT GCCAAGATGG TTGAAATTGA TTGCTCCTTA 
TTTATAAATT GCCACAGTAA GGATGTTGAT GACGGTATTT TATCAACTGT AAACAGACAG 
TCCCTAGTGT TTGACGGTAG ATTAATTGTG GATACACAAT TCAGAACTAC TGATACAAGT 
ATTTACTCTG CAGGGCCTAT GGCCAAGTTT TCAAGAAGGT TTGGAGAATC GAGAATGATG 
GAAGCTTTCA ACTCGTCTGA AGTTGGATCA AAAGTAGCAC AGTCGGTACT TTCAGCTCTT 
GGAGTAGCTG AAGATCCATT AACAGGATCT ATGCCAACAT TTGTAAAGAA CACATCAATT 
AGGTGTGTTC TTCCAGGGGG TGTAAGATTT TTCAGATGTA TGAATTCTCA ATTCGATGTT 
GAAAAATGTG TTAAATATCA AACAAATAAC GTTTTAGGAC CTCGTACAAT TAATGACATC 
ATAGAGCCTT TTAGATATAC AGGATTGTAT GTCAACAAAC AAACAAATTG TATTGACTGT 
ATTTGTTATC TTGGTCAGGA GCAAGTTGAA GAAGGAAACT TGGCAAGCTT GGTAGGTTTG 
CCATTATCCT ATTTCAATGA TTTAGTGCCA AGGTACAAGG ATGGATTTAT TTATGATTTC 
ATAGCATTTT TGAGAGAAAA TTGGGTTATG GCCATTCTTT GTCATAAGTT TATTTCTTTC 
AAGAATGAAA CTAAAAAGAA ACTTTTAAAT GAGGAGGATA ATATTGGTAA TATTAATGAA 
GTTTGTAATG AAATTGCAGA GCTAATATTG CATGGTGAAT CAGAAATTTT CACTGTTGAT 
CCAACCAAAA CTATTAAAGT TGAGAAGGAA GTTACCAGAG ATGCCAAAAT ATTTGTCCAA 
TCTGAGCTGA TAGATTTCCT TACTGTACAC CAAGAAGTTT TACACTCAAT ACCAGGAGTT 
TATTTTGTAC CAAAAAAACA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002671293.1
CDS	1..3504
Translation	MAEEDFIQSLSVGIRRAKSDEAPNIQKYISENSFLLTKRFGNYNLKTIIETGYISIVAEGDQGQYQGFASFEFEPSDTLDVEKYFSYLPLNVRNTIWIRLVHFEPLFGETVLIEMLNSIFITFSEVDNICFQPVRSNVQSGIDFFSQFSFFKQEIGSDVLVKSDTEKCIFVCHRDNLGRGVKRLSIRQAVVEDNDDIIPLLSSCENVTSYKTEGEYFLANLLFEQNDSKKILLATDTESGDIVGVMYITTELDIRQILSDYKTYNEGEPIYEDFIVEKQIGDNMVYHSNAFEIKLFYIQEEYKFRSIDLLRAAFKAFQESKYCIVGIKFEDHSKSEHPLLKQFIYIEPTQECSSHSLHILSQVSMVADFFVVKATETTLDKNSSPQETILSLLRQAEFHEEFIGAVAKSFKGTRYVTMLLFDSNSAVEGRNEIIGIAVVDTQVNEFLLRRHFDFTEYIDYSAYSSRGTCQAMITHFYIEQKFYRYSKLFLKEVMRKLEKHILYYGCYRGEIPNDSLNKELIYLKSTRQIEFPGALNESSSGRNSSLSDNDSVISGATVSSGGGSRVLTNVQYDSLYFINRRYLSEEKTEIFSRIVVIGGSKTALSFIKELSANSSLFFNNIIVVSPEGDVTKSPEFYADDTDEEFNNRELLNMISLPNIYFVKDKLEDIERVNKSVLLYENQSSFITYDILLLCTSRQYKMKPKYLELVGYPKKGILNLNPRVPVNETGEEIDLTEYFEMYLHENDSEERSDSSYIVVYGSSLDALCTVNTLLNRFNVEPSKLLLIFPDGKKCIFNENEEIEDRLDHILEVAGLKYFSQYTLEDFASDEEGNLVRIYLSPNEEKEEKKKHAKMVEIDCSLFINCHSKDVDDGILSTVNRQSLVFDGRLIVDTQFRTTDTSIYSAGPMAKFSRRFGESRMMEAFNSSEVGSKVAQSVLSALGVAEDPLTGSMPTFVKNTSIRCVLPGGVRFFRCMNSQFDVEKCVKYQTNNVLGPRTINDIIEPFRYTGLYVNKQTNCIDCICYLGQEQVEEGNLASLVGLPLSYFNDLVPRYKDGFIYDFIAFLRENWVMAILCHKFISFKNETKKKLLNEEDNIGNINEVCNEIAELILHGESEIFTVDPTKTIKVEKEVTRDAKIFVQSELIDFLTVHQEVLHSIPGVYFVPKKQ

Target ORF information:

RefSeq Version	XM_002671247.1
Organism	Naegleria gruberi strain NEG-M
Definition	Naegleria gruberi hypothetical protein (FM142), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002671247.1

ORF Insert Sequence:

ATGGCTGAAG AAGATTTTAT ACAATCACTT TCTGTTGGAA TTAGAAGAGC AAAATCAGAT 
GAAGCACCCA ACATACAAAA ATATATTAGT GAGAACAGCT TTCTTCTAAC AAAACGTTTC 
GGAAATTACA ATCTAAAAAC AATAATTGAG ACTGGCTACA TTTCTATTGT GGCTGAAGGT 
GATCAAGGAC AATATCAAGG ATTTGCATCA TTCGAATTTG AGCCATCAGA TACATTAGAT 
GTGGAGAAGT ACTTTTCCTA TCTTCCTCTG AATGTTCGGA ATACAATTTG GATTAGACTT 
GTTCATTTTG AACCCCTCTT TGGAGAGACA GTTTTAATTG AAATGCTAAA TTCAATTTTT 
ATTACTTTCT CTGAAGTTGA CAATATTTGT TTTCAACCAG TAAGATCAAA CGTTCAAAGT 
GGTATTGATT TCTTCTCTCA ATTCTCCTTC TTCAAACAAG AAATTGGATC AGATGTCCTT 
GTCAAGTCTG ACACTGAAAA ATGTATTTTT GTTTGCCACA GAGACAATCT AGGTCGTGGA 
GTTAAGCGTT TGTCAATAAG ACAAGCAGTT GTTGAGGATA ATGACGATAT AATTCCTCTA 
TTATCAAGTT GTGAGAATGT AACCTCCTAC AAAACAGAGG GCGAGTACTT TTTGGCTAAT 
CTCTTGTTTG AACAAAATGA TTCTAAAAAG ATTCTTCTAG CTACTGATAC AGAGAGTGGA 
GACATAGTAG GTGTCATGTA CATTACAACT GAGTTGGATA TTAGGCAAAT TCTCTCGGAT 
TACAAGACTT ATAATGAAGG AGAACCAATA TATGAGGATT TTATAGTTGA AAAACAAATT 
GGTGACAATA TGGTCTACCA TTCAAATGCC TTTGAAATTA AGCTCTTCTA CATTCAAGAG 
GAGTACAAGT TTAGAAGTAT TGATTTATTG AGAGCTGCAT TCAAGGCATT CCAAGAATCT 
AAATATTGCA TTGTGGGAAT CAAATTTGAG GACCATTCCA AATCTGAACA TCCTCTTTTA 
AAACAATTCA TCTATATTGA ACCAACTCAA GAATGTTCTA GCCATTCACT TCATATTCTT 
TCTCAAGTAT CCATGGTTGC AGACTTTTTC GTTGTAAAGG CCACAGAAAC AACACTGGAC 
AAAAATTCAT CACCACAAGA GACTATTCTG AGCTTATTGA GACAGGCAGA ATTTCATGAA 
GAGTTTATTG GTGCAGTAGC CAAAAGTTTT AAAGGAACAA GATATGTAAC CATGTTACTG 
TTTGATAGTA ACTCAGCAGT GGAAGGAAGA AATGAAATTA TTGGTATTGC AGTGGTGGAC 
ACCCAAGTTA ATGAATTTCT GCTTCGTAGA CACTTTGATT TTACAGAATA CATTGATTAT 
TCAGCATATA GCTCAAGAGG AACATGTCAA GCAATGATAA CACATTTTTA TATTGAGCAA 
AAGTTTTACA GATACTCCAA ATTGTTTTTG AAGGAAGTTA TGAGAAAATT AGAAAAACAT 
ATTCTCTACT ATGGCTGCTA CAGAGGTGAA ATTCCAAACG ATAGTCTTAA CAAGGAACTC 
ATTTACTTGA AATCGACCAG ACAAATAGAG TTTCCAGGAG CCTTAAATGA GAGTAGTAGT 
GGAAGAAATT CTTCATTATC TGACAATGAT TCCGTTATCT CTGGAGCTAC TGTTTCTAGC 
GGAGGTGGAT CGAGAGTTTT AACGAATGTA CAATACGATT CTCTCTACTT TATTAATAGA 
AGATATTTGA GTGAAGAAAA AACTGAAATA TTTTCCAGAA TTGTGGTTAT AGGTGGTTCA 
AAAACTGCAC TGAGTTTTAT TAAGGAACTT TCTGCCAACT CTTCTCTTTT CTTTAACAAT 
ATTATTGTCG TATCTCCTGA AGGTGATGTT ACCAAGAGCC CTGAATTTTA TGCCGATGAT 
ACGGATGAAG AATTCAACAA CAGAGAATTG CTTAATATGA TCTCTCTACC AAACATTTAC 
TTTGTGAAAG ATAAACTTGA AGATATAGAA AGAGTCAACA AGAGTGTCCT CTTATACGAA 
AATCAATCCA GCTTCATTAC GTATGATATT TTATTATTAT GTACAAGCAG ACAGTACAAG 
ATGAAACCAA AATATTTAGA ACTGGTTGGA TATCCCAAGA AAGGCATTCT CAATTTGAAC 
CCAAGAGTAC CTGTAAATGA AACAGGAGAA GAAATTGATC TAACCGAATA TTTTGAAATG 
TATTTACATG AAAATGATAG TGAAGAAAGA AGTGATAGTT CTTATATTGT AGTATATGGT 
TCCTCCTTAG ATGCATTGTG TACCGTTAAT ACTTTATTGA ACAGATTTAA TGTTGAGCCA 
TCAAAATTAT TACTCATTTT CCCTGATGGA AAGAAATGTA TTTTTAACGA GAATGAAGAA 
ATTGAAGATA GATTGGACCA TATTTTAGAA GTGGCCGGCC TAAAATACTT TTCTCAATAT 
ACATTGGAAG ATTTTGCCTC TGATGAAGAG GGAAATCTAG TGAGAATTTA CCTCTCACCA 
AATGAGGAAA AGGAAGAGAA GAAAAAACAT GCCAAGATGG TTGAAATTGA TTGCTCCTTA 
TTTATAAATT GCCACAGTAA GGATGTTGAT GACGGTATTT TATCAACTGT AAACAGACAG 
TCCCTAGTGT TTGACGGTAG ATTAATTGTG GATACACAAT TCAGAACTAC TGATACAAGT 
ATTTACTCTG CAGGGCCTAT GGCCAAGTTT TCAAGAAGGT TTGGAGAATC GAGAATGATG 
GAAGCTTTCA ACTCGTCTGA AGTTGGATCA AAAGTAGCAC AGTCGGTACT TTCAGCTCTT 
GGAGTAGCTG AAGATCCATT AACAGGATCT ATGCCAACAT TTGTAAAGAA CACATCAATT 
AGGTGTGTTC TTCCAGGGGG TGTAAGATTT TTCAGATGTA TGAATTCTCA ATTCGATGTT 
GAAAAATGTG TTAAATATCA AACAAATAAC GTTTTAGGAC CTCGTACAAT TAATGACATC 
ATAGAGCCTT TTAGATATAC AGGATTGTAT GTCAACAAAC AAACAAATTG TATTGACTGT 
ATTTGTTATC TTGGTCAGGA GCAAGTTGAA GAAGGAAACT TGGCAAGCTT GGTAGGTTTG 
CCATTATCCT ATTTCAATGA TTTAGTGCCA AGGTACAAGG ATGGATTTAT TTATGATTTC 
ATAGCATTTT TGAGAGAAAA TTGGGTTATG GCCATTCTTT GTCATAAGTT TATTTCTTTC 
AAGAATGAAA CTAAAAAGAA ACTTTTAAAT GAGGAGGATA ATATTGGTAA TATTAATGAA 
GTTTGTAATG AAATTGCAGA GCTAATATTG CATGGTGAAT CAGAAATTTT CACTGTTGAT 
CCAACCAAAA CTATTAAAGT TGAGAAGGAA GTTACCAGAG ATGCCAAAAT ATTTGTCCAA 
TCTGAGCTGA TAGATTTCCT TACTGTACAC CAAGAAGTTT TACACTCAAT ACCAGGAGTT 
TATTTTGTAC CAAAAAAACA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of Naegleria gruberi illuminates early eukaryotic versatility.
Cell140(5)631-42(2010 Mar)
Fritz-Laylin LK,Prochnik SE,Ginger ML,Dacks JB,Carpenter ML,Field MC,Kuo A,Paredez A,Chapman J,Pham J,Shu S,Neupane R,Cipriano M,Mancuso J,Tu H,Salamov A,Lindquist E,Shapiro H,Lucas S,Grigoriev IV,Cande WZ,Fulton C,Rokhsar DS,Dawson SC

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FM142 cDNA ORF clone, Naegleria gruberi strain NEG-M

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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