NAEGRDRAFT_80323 cDNA ORF clone, Naegleria gruberi strain NEG-M

Gene
Clones
gRNAs

Summary

Gene Symbol	NAEGRDRAFT_80323
Entrez Gene ID	8852048
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Naegleria gruberi strain NEG-M

mRNA and Protein(s)

mRNA	Protein	Name
XM_002675312.1	XP_002675358.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of Naegleria gruberi illuminates early eukaryotic versatility.
Fritz-Laylin LK, Prochnik SE, Ginger ML, Dacks JB, Carpenter ML, Field MC, Kuo A, Paredez A, Chapman J, Pham J, Shu S, Neupane R, Cipriano M, Mancuso J, Tu H, Salamov A, Lindquist E, Shapiro H, Lucas S, Grigoriev IV, Cande WZ, Fulton C, Rokhsar DS, Dawson SC
Cell140(5)631-42(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NAEGRDRAFT_80323 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAEGRDRAFT_80323 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONa03929	XM_002675312.1 Latest version!	Naegleria gruberi predicted protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAEGRDRAFT_80323 ( XM_002675312.1 ) cDNA ORF clone, Naegleria gruberi strain NEG-M -> XP_002675358.1 Naegleria gruberi predicted protein, mRNA.

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CloneID	ONa03929
Clone ID Related Accession (Same CDS sequence)	XM_002675312.1
Accession Version	XM_002675312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1269705600000
Organism	Naegleria gruberi strain NEG-M
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003163293). COMPLETENESS: incomplete on both ends.

ATGGCAGCTC TTGGACTCAT CTATTTTGTA TGTACACTCT TTATTGGAGT GGCTGCTTTT 
GTTTGGAGAA AATTCTTTTC TTCGAATGCT AATAATACGA CAACAACGAC TACAACTTCT 
TCATCATCGA CATCGACATT GGGTGATGAG TTGGTTCAGA AAGATGTTTC TTCTAAAGTT 
TCACCTAGAG TATTGGAAAC TGGAAGAAAG GAAGAGGAAA TATTGGATGA AACATTGAAA 
TCTGTATTGG AGGAATCTGG TATATTTATT GAAACTAATG ATGTGAAGGA GGAACCAATT 
AGTGTGGAAG AATCATCAGA CGAAACCTTG TCGGAAGTAA AGACAGACGA AATTCAAGAA 
GAATTGGAAG AAGTTTCTAC ACCACAACAA GAAGAAGAAT ATCAAGAAAC ATTTACTGAA 
GAAAAACAAA TTTCCATACC AGAAGAGTTA TCGGAAACTT GTCAAGAGGA AATTCTGTCC 
CAAACCAAAG ATATTACCGA CATGAAAGAG GAAGAACATT TGACTGATAT CACAGAAAGT 
CAGCAAAAGG ACATCACAGA AGAAGAAATA GTGGAAGATT CTACTACAGC AGTGGAGGAC 
AATGCTAAGG AGAGCATTGT TGTTCCAACA ATTGTTACAG AAGTAAAAGA GGAGAAATCA 
GAACCTAGTG AATTGTACAT AACTTGCCTG ATTGTATTGC AGGCAATTAT TCGAGGATAC 
TTACAAAGAC ATTCAATGGG ACAAGTGAAA GAAACTCTCT CAATGATAAC ACAAGGACTG 
TTAAACTCTT TCAATATCAG AAGTGAACTT GAAGAAGAAA AGAACAGTTT GACAACAATG 
CAATCAATTG TGAGAAATTC CAACTCTGTT AGGAAATTTA TGGATGAAAA ACAAAAAGTA 
CAACTCATTC AAGCTTTTAT TCAATCCAAG TTGGAATTTG TGAAATCTTC GGAACAAAAA 
GACAAGGTAA TAGAATTGCA ATCCATACTC AAATCAAAGC TCTTAATGAA GAATCTAAAG 
AGTTTATTAG AAAGTGTATC TACATTACAG AGCTTTTTGC ATTCTAAAAT AGTAGAGGAG 
AATTATGTGA GTACTGTATC AACAATCCAA TCTCTGCAAG CAAGAGTTCG ATCAAACTAT 
GAATCCAAAC AAGTGGAAGA AGGTTTAAAG AGCTTGGAGA TTTTGCAATC CAGATGCTTG 
AAATCTATAG TAGAAAAGGA TTTAAGGAAT CAATTAGAAA AAATCACCAT CCTACAATCA 
AAGGCACGCA CATTTATTAT TGGTAAAAAG TTAAGAAATG AAATTGAGAA TTGTAAATTA 
ATTCAAAGTG CTTGCACAGC ATACTTGAAG CAAAAACAAA TTCTCCATTT AGAAAACTTA 
TCATCAATGA TTCATTCATT TGCTGCTAGT AAGCAAGATA GACTCAACTA TAGAAATAAT 
GTGGATAATG TCAAAACTTT GGAATCCGTT ATGAGAGGAT ACTCCCAAAG AAACAGTATG 
ACAAATGTAT TGCACGCCAT TACAGACCTG CAATCTCATT TAATTGGACA AAATTCAAGA 
AAGTCGTTGC ATAAGAGTAT TGACAGAGCC AACACACTAT TGGCTCTTTC TAAGGCAAAA 
TGCTACAAGA AATCATTAAT GGTTGACAAG TCACGACTCG TAATATTACA AGCACTTGCT 
AGATCGAGCA TTACCAATAG AAAACTGAAT GCCAAACAAG CAAGCGAAGA GGACTTGGCA 
TTATTAGTCC AATCTCTACA TAATGGTTCA AAGTTGAGAT CAGAACATTC CGAGAAAGTG 
AGGAGTGTAA ACCTCATTAA TAGTTTACTC TCAGCAACTA AATCAAGAAA TACTTTCAAC 
AAAGAAAAGC TCTCAATTCA AACCATTCAA GCAGCTCTCA ACAGCCACAA AGTCCAACAT 
TTACACTCGA ACGACATGTC CAACATTGGC CTTATTCAAT CTGTGATAAT TGCAAAAAAG 
GCAAATGATG TCAAAACTAC AGCAACCAAC AATATAATTC TCCTACAATC ATTAATAAGA 
GGAAGGCAAA TTCAAAAAGA AAAGTCTCAC AACCTTGATC ATGCACATGT TTTGACAGCT 
TTCAGCAAAG GAAGATTGAT GTTACACCTT TTCGAGTCAC AAGTAGCTAC CACCACCAAA 
ATCCAAAGTT TTACTAGACA ATACACATCC CGAAAACAAA TGATGAAAGA GATTGATAAT 
GTTTGTCTCT TGGAATCTTT GTGCACTGGG TTTGTGGAAA GAAGAAATCT TTCAACAGAA 
ATTTCTAATA TTTTGCAATG TCAAAGTTTG ATTAGAGGAT TTATGACAAG AAAAGTCAGA 
GTTTCGGATG ATCAAATTGA TCAATTAGAG AAGAAAACAC ATTTCGTCTC CATGATACAA 
TCATCTATGG ATGGTAGAAA TATTCGAAAG ATTAGAGAAC AAGCAGCTGC TCATGTGAAA 
CTTTTCCAAG CTGTTTCTAG GGCCTTTTTA ACACTAGACA GCTATCAACA ACAACAAATC 
ATCTCCTTGC ACTTGCAATC TATTTGTAGG AGTGTTATGG AAAGGAAAGC ACTTTCAACT 
GACTTGAATA ATCTTCTTGG GTTGCAAGCT CTGTGCAGGA GTAGATTAAT TACTAACAGG 
TCCAAAGTTG ACAAAAATAG AGTTCAAATA GTGCAATCAA ATATTAGAAG ATATTTGGCA 
CACAAACACT TGGATCAAGA AAAATTGAGA GTAGCCAATT TACAAAGTTT GATTCGTGGA 
TTTGCTAACA AACAATTTGT CAAGGATCAA ATTGCTAGAG TGACAGTTAT GCAATCCATG 
ACTAGGGCAT ACTCTTACAG AAAACAAATG AAAAATTCAC AATCTAGAAT CAATATATTA 
CAAGCTAAGA TAAGAGGATA TTTAGAAAAA ACAAGAATTG AAGGACAAAA CTCTCTTTCT 
AACAATCTAG GATCTGTCAT GAAAGGATTT GCCCAAAGAG TCAAGCTATT CAAGGCAATA 
ACCAGCACTA TACCAACTAT TCAATCCTCT GTAAGACAAT TTGCATTGCG TAAAGGAATT 
AGTGAATCAT TGGGTGCTAT CATAAATATT CAATCACTGT GCAGAAAACA CTTGCAAAGT 
GAAAGCAAGC CCCTCTCTTT GAATAGAATA TTATCCCTTC AAGCACTAAT TAGAACATAC 
TCTAAGGGAA AACACACAAA TCAACAGTTG GGCAAGATTC CATATATTCA AGCTCTTTTC 
AGAGGAAAGC AGGCAAGAAA AACCATTGTC AAATCCTCAG ATGTTTCACC TATTCAAGCA 
GTTGCTAGAT CACTCAAACA GAGACGTGTT ATGGAACAAT CCGAACAAGG TATTACCAAA 
ATTAGTGCAG CTCTTCTTGG TTATTCCAAG AGAAGAGAAC TATTCCAAAG TATGAAGAAC 
ATAACTCACT GTCAAACTAT TCTTAAAACA GTGCTTTCAT CCAATGATTT GAGAAGCCAA 
ACGAAACAGT CTCTTGATTT GATATCTAAT TTGCAACAAA AGCTTAAAAC TAGATTGGAA 
TTGGAACGTT TTGAAAAGAA AAAGAAAAGT AGCCTACTTT TACAATCTTT GGCTAGAGGC 
TGCACTATAC GAAAGTTTAT TAGAAACTCT GTCAAACCTT CAGTTGAACT TATTGATTCA 
CTCTGTATTG GTCACATAGA AAGAGAAAAC CACAGAGACA AAGTTGAAAA AGCAACATTA 
TTACAAAGCA TCAGCAGAAG AGCCCAAGCA TGCAACCAAA TTTCTGAACA AAGACAAAAG 
TTATTACTTC TTCAAGCAGC TTTCAAATCA TGGAAAACTC GAGATACTTT CAAGAAGGAA 
CTAGAGGATA AAATTATTCA CGAGCTTGAA GATAAAATTA AGAGAGTTGT TGTCATTCAA 
GGCTTTGTAA GAGGTTACCT CTTCAGAAGA GATAACTTAC TCTACACAAA GAGGAGACAG 
GAATCCCCAA CTCAACAAAT TAGTGAACTA CCTTCCACTG CTGAAATTTT ACGTCAACTC 
TATCAAAATG ATGAGTTTGG AGACAATGAA GATAGTGAGG AAGAAGTACA ACTGGAATAT 
CCAGAAGAAG CTGATGACAT TCCAGAGCCA GTTATAATTG AGGAGCCACA TGTTGAAGTA 
ACTGTTAATA GTTTCCTGGA TACTCCATCT TCTCCTGAGG AGCTACCAAG TATTATTCCA 
ATTCCTCTTC ACAGAAGGAC TTCCAATGCA CCACTTCCTG CTATCAATAT TACAGCATCC 
ACCCCAACAC CAACAACAAC CGTAACTCCT TCTGTATTCT CTAAAGAACC AGTTGGTATT 
ACCTCCTCAA CTAAAAATAC TCGTCTTCCA AGTCAAAAAC CTCCAACCAG AAGACAACTC 
ATTCAAAATA TTATTGAGGA ATGTAAAAGA ACAAAGAATC CAGTCTTGAA TCTGTCTGGT 
TATGATTTGG AAACTTTACC ATACTCTGCC ATTGATTGCA AACATGTCAC AGAACTCATT 
TTGTCTCAAA ATAATCTTGT CACTTTACCT TCATGGTTTG TTGATACATT TAGTGATTTG 
AAAGTGTTAG ACTTGAGTGG AAATCAACTT TATGAGCTTC CAATTGATTT GGCAAGGCTT 
TCATTGACAA GTATTAATTT GAGTAATAAC AAGTTGGAAT TTTTACCTCA AGCATTGAAC 
GAAATGAACA CATTGACCAA GATTGATATT AGCGAAAACG TGCTTCTCAG TTTACCAAGT 
ACTATTAGTC ATTTGGTTAA TGTTGAAGAG TTGGATATTT CCAACAATTA CCTAATGACA 
TTGCCTGATG ATATCAAAAA ATTGAAAAAG CTCAAGAACT TCCAATATTA TAATAATGAA 
TATCTTCAAT CTGAAATTTC TAAAATTGCA GAGGTCATTA GCGCAGTATC TGGAGAAAAA 
GCAAAGACCA TTATTCAAAA ATTGAGAAAT GAAGACAGCA TGGGATATAA GGAAATTGGC 
TTATCCAACA AACATAAGGA AAACAACTTA TTCTTGGATC CTTATGATCT GACAGATATG 
TCAGAGGCAA CTGAAATGTA TAATGGTGAA GATGGTGAAT CAGATTATAG TATGACAGAT 
GATGTTGTAC TGTCTCCAAG ATTAATGAGA AATAATTCCT CTGCCACTAT GGTAGAAACA 
GACACCACTT CAACTCCAAT GACACCAATA TCTGGAAGAA ATAATGACTA TCTGGATACA 
CCTCCTACTC ATACACTTTC TGTAACAACA GAAGAATATG GAACACCATG TATCACCCCA 
CCAGCAATTT CAGATTCAGA TTCTGAATCT GATGTCACCC CACCAAAATA CAATCCACCT 
GTCACTATTC CTCAAATCAA ATTAGAAGTT ATTGAAAATT TGGATTCACC TTCATCATCT 
CCTGCTGAAC ATTCTCCAAT TTCTATGCAT CCTCCAAAAG TAATCATGAC TGAAGAGGAT 
AAGAAGGTCA ACTTTGGACA ACTCAACAAG GAAGGAAATG AAAAATCCTT GACAGATGTT 
GTTCACATGG AGCATCCACT ATTTGCTACT CCAAAGATTA GACCAGTTAG TGCCTCCTAC 
AAGAAGGTAG CACTTGAAGA GTCTGAAGAA GAAAGAAAAG AAAGAGAAGC AAAAGAAAAC 
AAAAAATCAA AGGAAAGAAT TAACATGGTT CTTGAAATTC TTGAAAGTGA AAGAAAATAT 
GTCAAATATC TGGATGTTTT GTGGGATTTA TTCTATGAAC CAATTGTAAA TAACTACTTC 
CCAGCTGATA AAAATAAGAA TCCAGAAGAA AGTGAACGAT TGGTACCTAA AATGATTGCC 
AAGAATTTCT TCCCTAATGA CTTGCTTACC ATCAAGACTT TCAACAAGAA CTTCCTTGTC 
AAACTTGAAG ACAGATTTAA GATTTACAAA GGTGATCCAA CAAAGATTTA TGACATGTTG 
ATTGGAGACT TGTTTGAAAA GATTGCTCCA TTCTTTAAAA TTTACACTTC CTATCTGAGT 
TCATACGAAT CTACAATGAA TAAGATTAGA GAATACAGAG CTGAAGACAA GATTTTTGAC 
AAGTGGCTTG ACAAGAGGAA AACCCATCCA AGATGTGCAG GTTTGGAAAT TGGTAGCTTT 
TTAATTATGC CAGTCCAAAG AATTCCAAGA TACAAGTTGC TATTGGAAAA TTTGTGCAAG 
AATACACCCC CAGATCATGC CGATTACTCC AACTTGATAT CAGCCATTGA GAAAATTACA 
GAATCTGCCA CTACTCAAAA TGCTAAAATT AAGGAAACCA ACAATCGTAT CAAAGTTTAT 
CAATTATCCA GACTAATGAA ATTGAGTGAT TTAGTCCAAC CACACAGAAG ATTGATCAGA 
GAGGGTGAAA TTGTTTTTGG AGATCAACCA AAGATTGAAT ATAAGGCCTA TCTCTTCAAT 
GACATTTTCG TATTCAGAGA AAAGAAGAGA CAAAAGACCA AAGTTGCCAA ATCAGTGAAA 
GCTTACTCAC TTCTCGATGC ATTCATTAGT GGAGGTGGTC AGAATAGACT CTCCATGATT 
CTACTCAACG CAGAAAAGAA CAAGACAGTA GAAAAGGAAA TCCTATTCCC TTCACATGAG 
GTTAAAATGG AGTGGGTATT GGAAATTGAG AAGGCACTCG AAGAGGTCAG AGAAAAACGT 
GAACTCATCT CGAAAGGAAC AAAGGCCATC GTGAGAACTA GTAGTAGAGC TGGTAGATTT 
TTCAGAGCTC TCAAATCACC AGGTAGAAAG GGTGATCTCT CTCCAACCTC TTCAAATCTT 
TCCTCCACCA CTATTGGTCA ACCTTCAACT CCATCAACAT CTGCTCCTGA GCTAAGAGGC 
GTTACCAGAA CCAATTCCAC TGTTTCAGAT CAATCCACAA CAAGTGTTGT CTCCAATTTG 
TCGGTCATTT CATCAACCTC TAATGTTGCC TCTTCCTCAA CTTCCTCTCT CAGCAGTAGT 
AGTGATGGAA AGAAAAAGTC AATCCTCGGT AAAATCATGA TGGGATTTGC CACCAACAAG 
AAAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002675358.1
CDS	1..7029
Translation	MAALGLIYFVCTLFIGVAAFVWRKFFSSNANNTTTTTTTSSSSTSTLGDELVQKDVSSKVSPRVLETGRKEEEILDETLKSVLEESGIFIETNDVKEEPISVEESSDETLSEVKTDEIQEELEEVSTPQQEEEYQETFTEEKQISIPEELSETCQEEILSQTKDITDMKEEEHLTDITESQQKDITEEEIVEDSTTAVEDNAKESIVVPTIVTEVKEEKSEPSELYITCLIVLQAIIRGYLQRHSMGQVKETLSMITQGLLNSFNIRSELEEEKNSLTTMQSIVRNSNSVRKFMDEKQKVQLIQAFIQSKLEFVKSSEQKDKVIELQSILKSKLLMKNLKSLLESVSTLQSFLHSKIVEENYVSTVSTIQSLQARVRSNYESKQVEEGLKSLEILQSRCLKSIVEKDLRNQLEKITILQSKARTFIIGKKLRNEIENCKLIQSACTAYLKQKQILHLENLSSMIHSFAASKQDRLNYRNNVDNVKTLESVMRGYSQRNSMTNVLHAITDLQSHLIGQNSRKSLHKSIDRANTLLALSKAKCYKKSLMVDKSRLVILQALARSSITNRKLNAKQASEEDLALLVQSLHNGSKLRSEHSEKVRSVNLINSLLSATKSRNTFNKEKLSIQTIQAALNSHKVQHLHSNDMSNIGLIQSVIIAKKANDVKTTATNNIILLQSLIRGRQIQKEKSHNLDHAHVLTAFSKGRLMLHLFESQVATTTKIQSFTRQYTSRKQMMKEIDNVCLLESLCTGFVERRNLSTEISNILQCQSLIRGFMTRKVRVSDDQIDQLEKKTHFVSMIQSSMDGRNIRKIREQAAAHVKLFQAVSRAFLTLDSYQQQQIISLHLQSICRSVMERKALSTDLNNLLGLQALCRSRLITNRSKVDKNRVQIVQSNIRRYLAHKHLDQEKLRVANLQSLIRGFANKQFVKDQIARVTVMQSMTRAYSYRKQMKNSQSRINILQAKIRGYLEKTRIEGQNSLSNNLGSVMKGFAQRVKLFKAITSTIPTIQSSVRQFALRKGISESLGAIINIQSLCRKHLQSESKPLSLNRILSLQALIRTYSKGKHTNQQLGKIPYIQALFRGKQARKTIVKSSDVSPIQAVARSLKQRRVMEQSEQGITKISAALLGYSKRRELFQSMKNITHCQTILKTVLSSNDLRSQTKQSLDLISNLQQKLKTRLELERFEKKKKSSLLLQSLARGCTIRKFIRNSVKPSVELIDSLCIGHIERENHRDKVEKATLLQSISRRAQACNQISEQRQKLLLLQAAFKSWKTRDTFKKELEDKIIHELEDKIKRVVVIQGFVRGYLFRRDNLLYTKRRQESPTQQISELPSTAEILRQLYQNDEFGDNEDSEEEVQLEYPEEADDIPEPVIIEEPHVEVTVNSFLDTPSSPEELPSIIPIPLHRRTSNAPLPAINITASTPTPTTTVTPSVFSKEPVGITSSTKNTRLPSQKPPTRRQLIQNIIEECKRTKNPVLNLSGYDLETLPYSAIDCKHVTELILSQNNLVTLPSWFVDTFSDLKVLDLSGNQLYELPIDLARLSLTSINLSNNKLEFLPQALNEMNTLTKIDISENVLLSLPSTISHLVNVEELDISNNYLMTLPDDIKKLKKLKNFQYYNNEYLQSEISKIAEVISAVSGEKAKTIIQKLRNEDSMGYKEIGLSNKHKENNLFLDPYDLTDMSEATEMYNGEDGESDYSMTDDVVLSPRLMRNNSSATMVETDTTSTPMTPISGRNNDYLDTPPTHTLSVTTEEYGTPCITPPAISDSDSESDVTPPKYNPPVTIPQIKLEVIENLDSPSSSPAEHSPISMHPPKVIMTEEDKKVNFGQLNKEGNEKSLTDVVHMEHPLFATPKIRPVSASYKKVALEESEEERKEREAKENKKSKERINMVLEILESERKYVKYLDVLWDLFYEPIVNNYFPADKNKNPEESERLVPKMIAKNFFPNDLLTIKTFNKNFLVKLEDRFKIYKGDPTKIYDMLIGDLFEKIAPFFKIYTSYLSSYESTMNKIREYRAEDKIFDKWLDKRKTHPRCAGLEIGSFLIMPVQRIPRYKLLLENLCKNTPPDHADYSNLISAIEKITESATTQNAKIKETNNRIKVYQLSRLMKLSDLVQPHRRLIREGEIVFGDQPKIEYKAYLFNDIFVFREKKRQKTKVAKSVKAYSLLDAFISGGGQNRLSMILLNAEKNKTVEKEILFPSHEVKMEWVLEIEKALEEVREKRELISKGTKAIVRTSSRAGRFFRALKSPGRKGDLSPTSSNLSSTTIGQPSTPSTSAPELRGVTRTNSTVSDQSTTSVVSNLSVISSTSNVASSSTSSLSSSSDGKKKSILGKIMMGFATNKKK

Target ORF information:

RefSeq Version	XM_002675312.1
Organism	Naegleria gruberi strain NEG-M
Definition	Naegleria gruberi predicted protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002675312.1

ORF Insert Sequence:

ATGGCAGCTC TTGGACTCAT CTATTTTGTA TGTACACTCT TTATTGGAGT GGCTGCTTTT 
GTTTGGAGAA AATTCTTTTC TTCGAATGCT AATAATACGA CAACAACGAC TACAACTTCT 
TCATCATCGA CATCGACATT GGGTGATGAG TTGGTTCAGA AAGATGTTTC TTCTAAAGTT 
TCACCTAGAG TATTGGAAAC TGGAAGAAAG GAAGAGGAAA TATTGGATGA AACATTGAAA 
TCTGTATTGG AGGAATCTGG TATATTTATT GAAACTAATG ATGTGAAGGA GGAACCAATT 
AGTGTGGAAG AATCATCAGA CGAAACCTTG TCGGAAGTAA AGACAGACGA AATTCAAGAA 
GAATTGGAAG AAGTTTCTAC ACCACAACAA GAAGAAGAAT ATCAAGAAAC ATTTACTGAA 
GAAAAACAAA TTTCCATACC AGAAGAGTTA TCGGAAACTT GTCAAGAGGA AATTCTGTCC 
CAAACCAAAG ATATTACCGA CATGAAAGAG GAAGAACATT TGACTGATAT CACAGAAAGT 
CAGCAAAAGG ACATCACAGA AGAAGAAATA GTGGAAGATT CTACTACAGC AGTGGAGGAC 
AATGCTAAGG AGAGCATTGT TGTTCCAACA ATTGTTACAG AAGTAAAAGA GGAGAAATCA 
GAACCTAGTG AATTGTACAT AACTTGCCTG ATTGTATTGC AGGCAATTAT TCGAGGATAC 
TTACAAAGAC ATTCAATGGG ACAAGTGAAA GAAACTCTCT CAATGATAAC ACAAGGACTG 
TTAAACTCTT TCAATATCAG AAGTGAACTT GAAGAAGAAA AGAACAGTTT GACAACAATG 
CAATCAATTG TGAGAAATTC CAACTCTGTT AGGAAATTTA TGGATGAAAA ACAAAAAGTA 
CAACTCATTC AAGCTTTTAT TCAATCCAAG TTGGAATTTG TGAAATCTTC GGAACAAAAA 
GACAAGGTAA TAGAATTGCA ATCCATACTC AAATCAAAGC TCTTAATGAA GAATCTAAAG 
AGTTTATTAG AAAGTGTATC TACATTACAG AGCTTTTTGC ATTCTAAAAT AGTAGAGGAG 
AATTATGTGA GTACTGTATC AACAATCCAA TCTCTGCAAG CAAGAGTTCG ATCAAACTAT 
GAATCCAAAC AAGTGGAAGA AGGTTTAAAG AGCTTGGAGA TTTTGCAATC CAGATGCTTG 
AAATCTATAG TAGAAAAGGA TTTAAGGAAT CAATTAGAAA AAATCACCAT CCTACAATCA 
AAGGCACGCA CATTTATTAT TGGTAAAAAG TTAAGAAATG AAATTGAGAA TTGTAAATTA 
ATTCAAAGTG CTTGCACAGC ATACTTGAAG CAAAAACAAA TTCTCCATTT AGAAAACTTA 
TCATCAATGA TTCATTCATT TGCTGCTAGT AAGCAAGATA GACTCAACTA TAGAAATAAT 
GTGGATAATG TCAAAACTTT GGAATCCGTT ATGAGAGGAT ACTCCCAAAG AAACAGTATG 
ACAAATGTAT TGCACGCCAT TACAGACCTG CAATCTCATT TAATTGGACA AAATTCAAGA 
AAGTCGTTGC ATAAGAGTAT TGACAGAGCC AACACACTAT TGGCTCTTTC TAAGGCAAAA 
TGCTACAAGA AATCATTAAT GGTTGACAAG TCACGACTCG TAATATTACA AGCACTTGCT 
AGATCGAGCA TTACCAATAG AAAACTGAAT GCCAAACAAG CAAGCGAAGA GGACTTGGCA 
TTATTAGTCC AATCTCTACA TAATGGTTCA AAGTTGAGAT CAGAACATTC CGAGAAAGTG 
AGGAGTGTAA ACCTCATTAA TAGTTTACTC TCAGCAACTA AATCAAGAAA TACTTTCAAC 
AAAGAAAAGC TCTCAATTCA AACCATTCAA GCAGCTCTCA ACAGCCACAA AGTCCAACAT 
TTACACTCGA ACGACATGTC CAACATTGGC CTTATTCAAT CTGTGATAAT TGCAAAAAAG 
GCAAATGATG TCAAAACTAC AGCAACCAAC AATATAATTC TCCTACAATC ATTAATAAGA 
GGAAGGCAAA TTCAAAAAGA AAAGTCTCAC AACCTTGATC ATGCACATGT TTTGACAGCT 
TTCAGCAAAG GAAGATTGAT GTTACACCTT TTCGAGTCAC AAGTAGCTAC CACCACCAAA 
ATCCAAAGTT TTACTAGACA ATACACATCC CGAAAACAAA TGATGAAAGA GATTGATAAT 
GTTTGTCTCT TGGAATCTTT GTGCACTGGG TTTGTGGAAA GAAGAAATCT TTCAACAGAA 
ATTTCTAATA TTTTGCAATG TCAAAGTTTG ATTAGAGGAT TTATGACAAG AAAAGTCAGA 
GTTTCGGATG ATCAAATTGA TCAATTAGAG AAGAAAACAC ATTTCGTCTC CATGATACAA 
TCATCTATGG ATGGTAGAAA TATTCGAAAG ATTAGAGAAC AAGCAGCTGC TCATGTGAAA 
CTTTTCCAAG CTGTTTCTAG GGCCTTTTTA ACACTAGACA GCTATCAACA ACAACAAATC 
ATCTCCTTGC ACTTGCAATC TATTTGTAGG AGTGTTATGG AAAGGAAAGC ACTTTCAACT 
GACTTGAATA ATCTTCTTGG GTTGCAAGCT CTGTGCAGGA GTAGATTAAT TACTAACAGG 
TCCAAAGTTG ACAAAAATAG AGTTCAAATA GTGCAATCAA ATATTAGAAG ATATTTGGCA 
CACAAACACT TGGATCAAGA AAAATTGAGA GTAGCCAATT TACAAAGTTT GATTCGTGGA 
TTTGCTAACA AACAATTTGT CAAGGATCAA ATTGCTAGAG TGACAGTTAT GCAATCCATG 
ACTAGGGCAT ACTCTTACAG AAAACAAATG AAAAATTCAC AATCTAGAAT CAATATATTA 
CAAGCTAAGA TAAGAGGATA TTTAGAAAAA ACAAGAATTG AAGGACAAAA CTCTCTTTCT 
AACAATCTAG GATCTGTCAT GAAAGGATTT GCCCAAAGAG TCAAGCTATT CAAGGCAATA 
ACCAGCACTA TACCAACTAT TCAATCCTCT GTAAGACAAT TTGCATTGCG TAAAGGAATT 
AGTGAATCAT TGGGTGCTAT CATAAATATT CAATCACTGT GCAGAAAACA CTTGCAAAGT 
GAAAGCAAGC CCCTCTCTTT GAATAGAATA TTATCCCTTC AAGCACTAAT TAGAACATAC 
TCTAAGGGAA AACACACAAA TCAACAGTTG GGCAAGATTC CATATATTCA AGCTCTTTTC 
AGAGGAAAGC AGGCAAGAAA AACCATTGTC AAATCCTCAG ATGTTTCACC TATTCAAGCA 
GTTGCTAGAT CACTCAAACA GAGACGTGTT ATGGAACAAT CCGAACAAGG TATTACCAAA 
ATTAGTGCAG CTCTTCTTGG TTATTCCAAG AGAAGAGAAC TATTCCAAAG TATGAAGAAC 
ATAACTCACT GTCAAACTAT TCTTAAAACA GTGCTTTCAT CCAATGATTT GAGAAGCCAA 
ACGAAACAGT CTCTTGATTT GATATCTAAT TTGCAACAAA AGCTTAAAAC TAGATTGGAA 
TTGGAACGTT TTGAAAAGAA AAAGAAAAGT AGCCTACTTT TACAATCTTT GGCTAGAGGC 
TGCACTATAC GAAAGTTTAT TAGAAACTCT GTCAAACCTT CAGTTGAACT TATTGATTCA 
CTCTGTATTG GTCACATAGA AAGAGAAAAC CACAGAGACA AAGTTGAAAA AGCAACATTA 
TTACAAAGCA TCAGCAGAAG AGCCCAAGCA TGCAACCAAA TTTCTGAACA AAGACAAAAG 
TTATTACTTC TTCAAGCAGC TTTCAAATCA TGGAAAACTC GAGATACTTT CAAGAAGGAA 
CTAGAGGATA AAATTATTCA CGAGCTTGAA GATAAAATTA AGAGAGTTGT TGTCATTCAA 
GGCTTTGTAA GAGGTTACCT CTTCAGAAGA GATAACTTAC TCTACACAAA GAGGAGACAG 
GAATCCCCAA CTCAACAAAT TAGTGAACTA CCTTCCACTG CTGAAATTTT ACGTCAACTC 
TATCAAAATG ATGAGTTTGG AGACAATGAA GATAGTGAGG AAGAAGTACA ACTGGAATAT 
CCAGAAGAAG CTGATGACAT TCCAGAGCCA GTTATAATTG AGGAGCCACA TGTTGAAGTA 
ACTGTTAATA GTTTCCTGGA TACTCCATCT TCTCCTGAGG AGCTACCAAG TATTATTCCA 
ATTCCTCTTC ACAGAAGGAC TTCCAATGCA CCACTTCCTG CTATCAATAT TACAGCATCC 
ACCCCAACAC CAACAACAAC CGTAACTCCT TCTGTATTCT CTAAAGAACC AGTTGGTATT 
ACCTCCTCAA CTAAAAATAC TCGTCTTCCA AGTCAAAAAC CTCCAACCAG AAGACAACTC 
ATTCAAAATA TTATTGAGGA ATGTAAAAGA ACAAAGAATC CAGTCTTGAA TCTGTCTGGT 
TATGATTTGG AAACTTTACC ATACTCTGCC ATTGATTGCA AACATGTCAC AGAACTCATT 
TTGTCTCAAA ATAATCTTGT CACTTTACCT TCATGGTTTG TTGATACATT TAGTGATTTG 
AAAGTGTTAG ACTTGAGTGG AAATCAACTT TATGAGCTTC CAATTGATTT GGCAAGGCTT 
TCATTGACAA GTATTAATTT GAGTAATAAC AAGTTGGAAT TTTTACCTCA AGCATTGAAC 
GAAATGAACA CATTGACCAA GATTGATATT AGCGAAAACG TGCTTCTCAG TTTACCAAGT 
ACTATTAGTC ATTTGGTTAA TGTTGAAGAG TTGGATATTT CCAACAATTA CCTAATGACA 
TTGCCTGATG ATATCAAAAA ATTGAAAAAG CTCAAGAACT TCCAATATTA TAATAATGAA 
TATCTTCAAT CTGAAATTTC TAAAATTGCA GAGGTCATTA GCGCAGTATC TGGAGAAAAA 
GCAAAGACCA TTATTCAAAA ATTGAGAAAT GAAGACAGCA TGGGATATAA GGAAATTGGC 
TTATCCAACA AACATAAGGA AAACAACTTA TTCTTGGATC CTTATGATCT GACAGATATG 
TCAGAGGCAA CTGAAATGTA TAATGGTGAA GATGGTGAAT CAGATTATAG TATGACAGAT 
GATGTTGTAC TGTCTCCAAG ATTAATGAGA AATAATTCCT CTGCCACTAT GGTAGAAACA 
GACACCACTT CAACTCCAAT GACACCAATA TCTGGAAGAA ATAATGACTA TCTGGATACA 
CCTCCTACTC ATACACTTTC TGTAACAACA GAAGAATATG GAACACCATG TATCACCCCA 
CCAGCAATTT CAGATTCAGA TTCTGAATCT GATGTCACCC CACCAAAATA CAATCCACCT 
GTCACTATTC CTCAAATCAA ATTAGAAGTT ATTGAAAATT TGGATTCACC TTCATCATCT 
CCTGCTGAAC ATTCTCCAAT TTCTATGCAT CCTCCAAAAG TAATCATGAC TGAAGAGGAT 
AAGAAGGTCA ACTTTGGACA ACTCAACAAG GAAGGAAATG AAAAATCCTT GACAGATGTT 
GTTCACATGG AGCATCCACT ATTTGCTACT CCAAAGATTA GACCAGTTAG TGCCTCCTAC 
AAGAAGGTAG CACTTGAAGA GTCTGAAGAA GAAAGAAAAG AAAGAGAAGC AAAAGAAAAC 
AAAAAATCAA AGGAAAGAAT TAACATGGTT CTTGAAATTC TTGAAAGTGA AAGAAAATAT 
GTCAAATATC TGGATGTTTT GTGGGATTTA TTCTATGAAC CAATTGTAAA TAACTACTTC 
CCAGCTGATA AAAATAAGAA TCCAGAAGAA AGTGAACGAT TGGTACCTAA AATGATTGCC 
AAGAATTTCT TCCCTAATGA CTTGCTTACC ATCAAGACTT TCAACAAGAA CTTCCTTGTC 
AAACTTGAAG ACAGATTTAA GATTTACAAA GGTGATCCAA CAAAGATTTA TGACATGTTG 
ATTGGAGACT TGTTTGAAAA GATTGCTCCA TTCTTTAAAA TTTACACTTC CTATCTGAGT 
TCATACGAAT CTACAATGAA TAAGATTAGA GAATACAGAG CTGAAGACAA GATTTTTGAC 
AAGTGGCTTG ACAAGAGGAA AACCCATCCA AGATGTGCAG GTTTGGAAAT TGGTAGCTTT 
TTAATTATGC CAGTCCAAAG AATTCCAAGA TACAAGTTGC TATTGGAAAA TTTGTGCAAG 
AATACACCCC CAGATCATGC CGATTACTCC AACTTGATAT CAGCCATTGA GAAAATTACA 
GAATCTGCCA CTACTCAAAA TGCTAAAATT AAGGAAACCA ACAATCGTAT CAAAGTTTAT 
CAATTATCCA GACTAATGAA ATTGAGTGAT TTAGTCCAAC CACACAGAAG ATTGATCAGA 
GAGGGTGAAA TTGTTTTTGG AGATCAACCA AAGATTGAAT ATAAGGCCTA TCTCTTCAAT 
GACATTTTCG TATTCAGAGA AAAGAAGAGA CAAAAGACCA AAGTTGCCAA ATCAGTGAAA 
GCTTACTCAC TTCTCGATGC ATTCATTAGT GGAGGTGGTC AGAATAGACT CTCCATGATT 
CTACTCAACG CAGAAAAGAA CAAGACAGTA GAAAAGGAAA TCCTATTCCC TTCACATGAG 
GTTAAAATGG AGTGGGTATT GGAAATTGAG AAGGCACTCG AAGAGGTCAG AGAAAAACGT 
GAACTCATCT CGAAAGGAAC AAAGGCCATC GTGAGAACTA GTAGTAGAGC TGGTAGATTT 
TTCAGAGCTC TCAAATCACC AGGTAGAAAG GGTGATCTCT CTCCAACCTC TTCAAATCTT 
TCCTCCACCA CTATTGGTCA ACCTTCAACT CCATCAACAT CTGCTCCTGA GCTAAGAGGC 
GTTACCAGAA CCAATTCCAC TGTTTCAGAT CAATCCACAA CAAGTGTTGT CTCCAATTTG 
TCGGTCATTT CATCAACCTC TAATGTTGCC TCTTCCTCAA CTTCCTCTCT CAGCAGTAGT 
AGTGATGGAA AGAAAAAGTC AATCCTCGGT AAAATCATGA TGGGATTTGC CACCAACAAG 
AAAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of Naegleria gruberi illuminates early eukaryotic versatility.
Cell140(5)631-42(2010 Mar)
Fritz-Laylin LK,Prochnik SE,Ginger ML,Dacks JB,Carpenter ML,Field MC,Kuo A,Paredez A,Chapman J,Pham J,Shu S,Neupane R,Cipriano M,Mancuso J,Tu H,Salamov A,Lindquist E,Shapiro H,Lucas S,Grigoriev IV,Cande WZ,Fulton C,Rokhsar DS,Dawson SC

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NAEGRDRAFT_80323 cDNA ORF clone, Naegleria gruberi strain NEG-M

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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