NAEGRDRAFT_78512 cDNA ORF clone, Naegleria gruberi strain NEG-M

Gene
Clones
gRNAs

Summary

Gene Symbol	NAEGRDRAFT_78512
Entrez Gene ID	8848413
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Naegleria gruberi strain NEG-M

mRNA and Protein(s)

mRNA	Protein	Name
XM_002681047.1	XP_002681093.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of Naegleria gruberi illuminates early eukaryotic versatility.
Fritz-Laylin LK, Prochnik SE, Ginger ML, Dacks JB, Carpenter ML, Field MC, Kuo A, Paredez A, Chapman J, Pham J, Shu S, Neupane R, Cipriano M, Mancuso J, Tu H, Salamov A, Lindquist E, Shapiro H, Lucas S, Grigoriev IV, Cande WZ, Fulton C, Rokhsar DS, Dawson SC
Cell140(5)631-42(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NAEGRDRAFT_78512 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAEGRDRAFT_78512 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONa00658	XM_002681047.1 Latest version!	Naegleria gruberi hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAEGRDRAFT_78512 ( XM_002681047.1 ) cDNA ORF clone, Naegleria gruberi strain NEG-M -> XP_002681093.1 Naegleria gruberi hypothetical protein, mRNA.

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CloneID	ONa00658
Clone ID Related Accession (Same CDS sequence)	XM_002681047.1
Accession Version	XM_002681047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1269705600000
Organism	Naegleria gruberi strain NEG-M
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003163320). COMPLETENESS: incomplete on the 5' end.

ATGTTGAATT TATTCAAGAA GAAGATTACC TATCATGCCA CATCTGGTTT GGTTGCATCT 
TCTTCATCGC CGACGAGTGC CCTATTGTTT GGCAGTGGTG CTAATACAAC AGTAACGAAT 
AATATTCTTC TGCCATCAAT AGTCAACAAT AACAACAGTG GGACAACAAG AGGCTTTAAA 
CAAAAGTTTG GTAGAGTTTC ATCATCCAAC AATACGGAAG ATAAACAACA ACAACATTCA 
TCTCAACATG GATTTTCTAC TAATAGTGGT GAAACAAGAC AACCAACATC TAAAAAACCA 
TTCAAGTCTA ATTTCCAACA AAATTCCAAC AATCATCACA ACAAACCACA CCACCAGAAA 
TCACAACAAA ATTATAAGGA AAAACATTCA AACAATACCA CTTCTACCAC ATCTTTCCTA 
AAAGACCATC ACGAAGAACC ATCAAGAAAC TTTAAATTCA ATAATAACCA ACAACATTTT 
GATAAGAGTC AAAGAAAAGG AGGATTTAAT AAGAGAGATG ATAAAGCTCA ATTTCAACAT 
CACAAAAAGC ATAATATGGA AAGGAAACCA AAGAAACAAC AGTCAGAAAC TCCTTTACCC 
GATGTTGTTT CTAATGTTGA AGAGCCTATC AAAGAGGAAA AGAAAAGTTT TGTTGCTCTC 
AATTATGACG ATATTGATAT GAACCTTAAA GGTGAAGATG AAACACACCA AACATCTGCA 
AGTGATGTCT CTCCTTTCTA TGAAGGGAAT GAACATTCAT CTGAATTCAC ACCTTTCACA 
AAAGAATTGG AAATTAATGT AGACATTTTA TTGAAACGAG TTTTAGAAAA GGTCAAAACA 
CAAAATGCTG TAGAAATTGA TAAGGAGAAA GATGGTCTTT CTTCACTTCT CAGAGCTACT 
GATTCTGCAA TCAAGGAAGA GGATGACGAT ATTTTCCAAC CACGTGATTT TAGTCTTATC 
GAGGAAACTG ATGAAGCAGA AGAAGAACAA GATTTTGTTG GAAAGAAATA TGATACACTT 
CATAAGAAAC CACTAGAATT GAAGAAGAAA GCCTCTCGTG AAAAGGAAGA ATTCAGTGAA 
AAACAAAGTG CTGAAGTACT GACAAATGCC AAATACATTC CATCTGTGGG TATAAAATAT 
AGAAGTAAGA ATAATGATGA ATCACTTCCA GCTCTTCACA AATACCTAGT TGATTTTGGA 
GATTCAGAAT ACTTGCAATT GGCTGAAACT CATCCATCTG AAAAGATGTA TGTCGGAGAT 
TCAGATATTA ATCAAGTACC AACTTTGAAA AATGGGCTCA ATAGAGTTTT AACTTCACCT 
GGTGTACACA GAATGTATGA TGGTAAAACT AAAACTCATT TCTTCCATCC ATACATGAGA 
CAACTCCACA AACCAGAAGA AATCAACTTT AATAATTTGA CACCTTTCAT TCCACCATCC 
CAAGATACTA CCCTCCACAA ATTAATGGAT CAAAATCCTT GCAGATATCA ATCATCAACA 
AGTTCCATAA CCTCACCTCT GGTTTCCCTC TATCTTGCAC TCTCTAACTA CAAACCAACA 
AGAGCAAGAA ATTTGGAAAA TTTCAAATAT CTTACAAGCT CATTCACTCC TGCTGTTCGT 
AAGCCAGTCG TTTTTATTCT TCGTCCAAAG CTTTCCAAAC TCGGACATAC TTTCTACTCA 
ATAGATTCAT ACAAATTCCT TTTTGAGCCT CGATCAAATC AAATTTTGAT GGACCTTGGT 
AAAGTAATGG AAAAGCTTTT TGTTATGGAA CCAGAAGATT TTGAAAAGAG ATTTGTTAAA 
CAATATTGTG GAGAAGAACA AACAGTAGAA GATGAAGAAG TATACAGATA CCTTCGCCAC 
GGAAGTTTCC TTTTGAGATC ACAGTTGGAT TGTTACCATC CTCAATATGG TGTTTTTGAT 
ATTAAGACAA GAGCAACAAA TCCAATCAGA ATCAACATGG AAAAATATCA AAAATTCACA 
AACATCAAAC TGAAGATGAT TAAGGGTAAC TATAACTCAT ACGAAAGAGA ATTTTATGAT 
ATGGTTAGGT CTGTATTCAT AAAGTATAGT ATGCAGGGTA GAATTGGTGG CATGGACGGC 
ATGTTCTTGG CCTATCACAA TACTGTCGAA CTTTTTGGTT TTGAATACAT CAAGTTGGGC 
GAAATTGACA AACATGTATT CGGTAGTCCA CATATGGCAG AAAGATTCTT CAGTCTTTTA 
CTCGATTTAT TAGGTGAAGT TTTGGATAGA ATTACTGAAA AATTTGCTGG TCAAGCTCTC 
AAAGTAACTT TCCGTCCAGT TAAAGTAGAA CCATACTTTA TTGACATTTT TGTTGAACCA 
GTACATGGTG ATTTAGGATG GAATGAAAGA AAGCCAACAG AAGAAGAAAA GATGAAATGG 
GAGTCTGATC GATTCAATGC TAATCCATTC GCTGACATGA TAGAAGATAC TGTTTATTGT 
TATCGTTTAT CATGCCAACC AATGGTAAAT GGAGCTGTTT GCAGAAACTA TGTCGAATTC 
AAGGAAGAAG ACAAGGTTGA CATTTTGTAC TGTTTGGAAG AAATGGGTGA TACCACAACT 
GACAAATTCT TATTGAATGA GTACATTCTC ACTTTAAAGA AGGCCTTCTT TGTTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002681093.1
CDS	1..2640
Translation	MLNLFKKKITYHATSGLVASSSSPTSALLFGSGANTTVTNNILLPSIVNNNNSGTTRGFKQKFGRVSSSNNTEDKQQQHSSQHGFSTNSGETRQPTSKKPFKSNFQQNSNNHHNKPHHQKSQQNYKEKHSNNTTSTTSFLKDHHEEPSRNFKFNNNQQHFDKSQRKGGFNKRDDKAQFQHHKKHNMERKPKKQQSETPLPDVVSNVEEPIKEEKKSFVALNYDDIDMNLKGEDETHQTSASDVSPFYEGNEHSSEFTPFTKELEINVDILLKRVLEKVKTQNAVEIDKEKDGLSSLLRATDSAIKEEDDDIFQPRDFSLIEETDEAEEEQDFVGKKYDTLHKKPLELKKKASREKEEFSEKQSAEVLTNAKYIPSVGIKYRSKNNDESLPALHKYLVDFGDSEYLQLAETHPSEKMYVGDSDINQVPTLKNGLNRVLTSPGVHRMYDGKTKTHFFHPYMRQLHKPEEINFNNLTPFIPPSQDTTLHKLMDQNPCRYQSSTSSITSPLVSLYLALSNYKPTRARNLENFKYLTSSFTPAVRKPVVFILRPKLSKLGHTFYSIDSYKFLFEPRSNQILMDLGKVMEKLFVMEPEDFEKRFVKQYCGEEQTVEDEEVYRYLRHGSFLLRSQLDCYHPQYGVFDIKTRATNPIRINMEKYQKFTNIKLKMIKGNYNSYEREFYDMVRSVFIKYSMQGRIGGMDGMFLAYHNTVELFGFEYIKLGEIDKHVFGSPHMAERFFSLLLDLLGEVLDRITEKFAGQALKVTFRPVKVEPYFIDIFVEPVHGDLGWNERKPTEEEKMKWESDRFNANPFADMIEDTVYCYRLSCQPMVNGAVCRNYVEFKEEDKVDILYCLEEMGDTTTDKFLLNEYILTLKKAFFVE

Target ORF information:

RefSeq Version	XM_002681047.1
Organism	Naegleria gruberi strain NEG-M
Definition	Naegleria gruberi hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002681047.1

ORF Insert Sequence:

ATGTTGAATT TATTCAAGAA GAAGATTACC TATCATGCCA CATCTGGTTT GGTTGCATCT 
TCTTCATCGC CGACGAGTGC CCTATTGTTT GGCAGTGGTG CTAATACAAC AGTAACGAAT 
AATATTCTTC TGCCATCAAT AGTCAACAAT AACAACAGTG GGACAACAAG AGGCTTTAAA 
CAAAAGTTTG GTAGAGTTTC ATCATCCAAC AATACGGAAG ATAAACAACA ACAACATTCA 
TCTCAACATG GATTTTCTAC TAATAGTGGT GAAACAAGAC AACCAACATC TAAAAAACCA 
TTCAAGTCTA ATTTCCAACA AAATTCCAAC AATCATCACA ACAAACCACA CCACCAGAAA 
TCACAACAAA ATTATAAGGA AAAACATTCA AACAATACCA CTTCTACCAC ATCTTTCCTA 
AAAGACCATC ACGAAGAACC ATCAAGAAAC TTTAAATTCA ATAATAACCA ACAACATTTT 
GATAAGAGTC AAAGAAAAGG AGGATTTAAT AAGAGAGATG ATAAAGCTCA ATTTCAACAT 
CACAAAAAGC ATAATATGGA AAGGAAACCA AAGAAACAAC AGTCAGAAAC TCCTTTACCC 
GATGTTGTTT CTAATGTTGA AGAGCCTATC AAAGAGGAAA AGAAAAGTTT TGTTGCTCTC 
AATTATGACG ATATTGATAT GAACCTTAAA GGTGAAGATG AAACACACCA AACATCTGCA 
AGTGATGTCT CTCCTTTCTA TGAAGGGAAT GAACATTCAT CTGAATTCAC ACCTTTCACA 
AAAGAATTGG AAATTAATGT AGACATTTTA TTGAAACGAG TTTTAGAAAA GGTCAAAACA 
CAAAATGCTG TAGAAATTGA TAAGGAGAAA GATGGTCTTT CTTCACTTCT CAGAGCTACT 
GATTCTGCAA TCAAGGAAGA GGATGACGAT ATTTTCCAAC CACGTGATTT TAGTCTTATC 
GAGGAAACTG ATGAAGCAGA AGAAGAACAA GATTTTGTTG GAAAGAAATA TGATACACTT 
CATAAGAAAC CACTAGAATT GAAGAAGAAA GCCTCTCGTG AAAAGGAAGA ATTCAGTGAA 
AAACAAAGTG CTGAAGTACT GACAAATGCC AAATACATTC CATCTGTGGG TATAAAATAT 
AGAAGTAAGA ATAATGATGA ATCACTTCCA GCTCTTCACA AATACCTAGT TGATTTTGGA 
GATTCAGAAT ACTTGCAATT GGCTGAAACT CATCCATCTG AAAAGATGTA TGTCGGAGAT 
TCAGATATTA ATCAAGTACC AACTTTGAAA AATGGGCTCA ATAGAGTTTT AACTTCACCT 
GGTGTACACA GAATGTATGA TGGTAAAACT AAAACTCATT TCTTCCATCC ATACATGAGA 
CAACTCCACA AACCAGAAGA AATCAACTTT AATAATTTGA CACCTTTCAT TCCACCATCC 
CAAGATACTA CCCTCCACAA ATTAATGGAT CAAAATCCTT GCAGATATCA ATCATCAACA 
AGTTCCATAA CCTCACCTCT GGTTTCCCTC TATCTTGCAC TCTCTAACTA CAAACCAACA 
AGAGCAAGAA ATTTGGAAAA TTTCAAATAT CTTACAAGCT CATTCACTCC TGCTGTTCGT 
AAGCCAGTCG TTTTTATTCT TCGTCCAAAG CTTTCCAAAC TCGGACATAC TTTCTACTCA 
ATAGATTCAT ACAAATTCCT TTTTGAGCCT CGATCAAATC AAATTTTGAT GGACCTTGGT 
AAAGTAATGG AAAAGCTTTT TGTTATGGAA CCAGAAGATT TTGAAAAGAG ATTTGTTAAA 
CAATATTGTG GAGAAGAACA AACAGTAGAA GATGAAGAAG TATACAGATA CCTTCGCCAC 
GGAAGTTTCC TTTTGAGATC ACAGTTGGAT TGTTACCATC CTCAATATGG TGTTTTTGAT 
ATTAAGACAA GAGCAACAAA TCCAATCAGA ATCAACATGG AAAAATATCA AAAATTCACA 
AACATCAAAC TGAAGATGAT TAAGGGTAAC TATAACTCAT ACGAAAGAGA ATTTTATGAT 
ATGGTTAGGT CTGTATTCAT AAAGTATAGT ATGCAGGGTA GAATTGGTGG CATGGACGGC 
ATGTTCTTGG CCTATCACAA TACTGTCGAA CTTTTTGGTT TTGAATACAT CAAGTTGGGC 
GAAATTGACA AACATGTATT CGGTAGTCCA CATATGGCAG AAAGATTCTT CAGTCTTTTA 
CTCGATTTAT TAGGTGAAGT TTTGGATAGA ATTACTGAAA AATTTGCTGG TCAAGCTCTC 
AAAGTAACTT TCCGTCCAGT TAAAGTAGAA CCATACTTTA TTGACATTTT TGTTGAACCA 
GTACATGGTG ATTTAGGATG GAATGAAAGA AAGCCAACAG AAGAAGAAAA GATGAAATGG 
GAGTCTGATC GATTCAATGC TAATCCATTC GCTGACATGA TAGAAGATAC TGTTTATTGT 
TATCGTTTAT CATGCCAACC AATGGTAAAT GGAGCTGTTT GCAGAAACTA TGTCGAATTC 
AAGGAAGAAG ACAAGGTTGA CATTTTGTAC TGTTTGGAAG AAATGGGTGA TACCACAACT 
GACAAATTCT TATTGAATGA GTACATTCTC ACTTTAAAGA AGGCCTTCTT TGTTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of Naegleria gruberi illuminates early eukaryotic versatility.
Cell140(5)631-42(2010 Mar)
Fritz-Laylin LK,Prochnik SE,Ginger ML,Dacks JB,Carpenter ML,Field MC,Kuo A,Paredez A,Chapman J,Pham J,Shu S,Neupane R,Cipriano M,Mancuso J,Tu H,Salamov A,Lindquist E,Shapiro H,Lucas S,Grigoriev IV,Cande WZ,Fulton C,Rokhsar DS,Dawson SC

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NAEGRDRAFT_78512 cDNA ORF clone, Naegleria gruberi strain NEG-M

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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