PC cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	PC
Entrez Gene ID	100015028
Full Name	pyruvate carboxylase
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007502651.2	XP_007502713.1	pyruvate carboxylase, mitochondrial isoform X2
XM_001364491.3	XP_001364528.1	pyruvate carboxylase, mitochondrial isoform X2
XM_007502650.2	XP_007502712.1	pyruvate carboxylase, mitochondrial isoform X1
XM_007502652.2	XP_007502714.1	pyruvate carboxylase, mitochondrial isoform X2

Pathways from BioSystems
Gene Ontology

The following PC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc31725	XM_007502650.2 Latest version!	Monodelphis domestica pyruvate carboxylase (PC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMc31726	XM_001364491.3 Latest version!	Monodelphis domestica pyruvate carboxylase (PC), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMc31726	XM_007502651.2 Latest version!	Monodelphis domestica pyruvate carboxylase (PC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMc31726	XM_007502652.2 Latest version!	Monodelphis domestica pyruvate carboxylase (PC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PC ( XM_007502650.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007502712.1 Monodelphis domestica pyruvate carboxylase (PC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMc31725
Clone ID Related Accession (Same CDS sequence)	XM_007502650.2
Accession Version	XM_007502650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008808.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007502650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGA AGTTTCATTT GGCCCAAAGG GGCCTAAGGC TCCTAAGTAT CCGACATGCC 
TCCATGATGC CCCCCTCAAA CGCCCAACAC ATCAAGTACA AGCCCATCAA GAAAGTCATG 
GTGGCCAACC GAGGTGAGAT AGCCATTCGA GTGTTCCGGG CCTGCACGGA GCTTGGCATC 
CGCACCGTGG CCGTGTATTC CGAGCAGGAC ACTGGGCAGA TGCATCGGCA GAAGGCAGAT 
GAGGCTTACC TCATTGGCCG TGGTCTTAGC CCAGTGCAGG CCTACCTGAA CATCGCAGAC 
ATCATCAAGG TGGCCAAGGA GAATGATGTC GATGCCATTC ACCCCGGCTA TGGGTTCTTG 
TCGGAGCGTG CTGATTTTGC TCAGGCCTGT CTGGATGCTG GGATCAAATT CATTGGCCCC 
AGCCCTGAAG TGGTGCGCAA GATGGGGGAC AAGGTGAGGG CCCGGCCCAT TCCCATTGCT 
GCAGGTGTCC CAGTGGTTCC AGGCACAGAC ACCCCCATCT CCTCCTTACA CGAGGCTCAC 
GAATTCTCCA AAACCTATGG CCTCCCAATT ATCTTCAAAG CTGCCTATGG AGGCGGGGGT 
CGTGGCATGA GGGTCGTACA AAACTTTGAG GAATTGGATG AGAATTTTAA CCGGGCCTAT 
TCCGAGGCCC TGGCCGCCTT TGGGGATGGG ACTCTGTTTG TGGAAAAATT CATTGAGAAT 
CCACGCCACA TTGAAGTGCA AATTCTGGGG GACCAGCATG GGAACATTGT GCACCTCTAC 
GAACGGGACT GCTCGATCCA GCGGAGGCAT CAGAAGGTGG TGGAGATTGC TCCGGCCACT 
CTCCTGGACA CCCAGCTCCG GAATCGCCTG ACCAGTGATT CGGTCAAGTT AGGCAAGCAG 
GTGGGCTATG AGAACGCTGG CACAGTGGAG TTCCTCATAG ACAAACACGG CAAACACTAT 
TTCATCGAGG TCAATTCTCG CCTACAAGTG GAGCACACGG TCACCGAGGA GATCACCCAC 
GTAGACCTGG TCCATGCCCA GATCCATGTA GCTGAGGGCC GGAGCCTGCC GGATCTGGGC 
CTTCTGCAAG AGAATATTCG AATCAGCGGT TGTGCAATCC AGTGTCGGGT GACAACAGAG 
GACCCGTCTC GAGGCTTCCA GCCAGACACT GGCCGGATCG AGGTGTTCCG TAGTGGTGAG 
GGGATGGGCA TCCGCTTGGA CAACGCCTCT GCCTTCCAAG GGTCAGTCAT CTCCCCGCAC 
TACGATTCCC TGTTGGTCAA AGTCATAGCC CATGGAAAGG ACCACCCCAC GGCGGCCACC 
AAGATGAGCC GGGCACTAGC CGAGTTCCGG GTCCGAGGGG TCAAGACCAA CATCCCCTTC 
CTGCAGAATG TTCTCAACAA CCAGCAGTTC CTGGCGGGCA CCGTGGATAC CCAGTTCATC 
GATTCAAACC CTGAGCTCTT CCAGATGCGG CCTGTTCAGA ACCGGGCCCA GAAGCTGCTG 
CACTACCTGG GTCACGTCAT GGTGAATGGG CCCCAGACAC CGATCCCGGT CAAGGCAAGC 
TCCAGCCCCA CGGATCCCAT CATCCCCCCC GTCCCCATGG GCCCACCTCC TCCTGGTTTC 
AGAGACATCC TGCTGCGTGA GGGTCCCGAG GGCTTTGCCC GGGCCGTGCG GAACCACAAG 
GGGCTGCTGC TAATGGACAC GACCTTCCGG GATGCCCATC AGTCCCTGCT GGCCACACGA 
GTGAGGACCT ACGACCTCAA GAAGATCGCC CCTTATGTCG CCCACAGTTT CAACAGCCTT 
TTCAGTCTGG AGAACTGGGG GGGAGCCACG TTTGATGTTG CCATGCGCTT CCTGTACGAG 
TGTCCGTGGC GGAGGCTACA GGAGCTCAGA GAGCTCATCC CCAACATCCC CTTCCAGATG 
CTGCTCCGGG GTGCCAACGC TGTGGGCTAC ACCAACTACC CGGACAACGT GGTCTTTAAG 
TTCTGTGAGG TGGCCAAGGA GAACGGGATG GACGTGTTCC GTGTCTTTGA TTCCCTCAAC 
TACATGCCCA ATCTGCTGCT GGGCATGGAG GCGGCAGGCA GTGCCGGAGG TGTGGTGGAG 
GCAGCCATCT CCTACACGGG CGACGTGGCC GACCCCACGC GCACCAAGTA TTCCCTGAAG 
TACTACATGG ACCTGGCTGA GGAGCTGGTG CGGGCTGGGA CACACATCCT GAGCATCAAG 
GACATGGCAG GCCTTCTGAA GCCGGCTGCA TGCACCATGC TGGTGGGTGC CCTCCGAGAC 
CGCTTCCCAG AGCTCCCCCT GCACATCCAC ACCCATGACA CGGCCGGCTC AGGTGTGGCC 
AATATGCTGG CCTGTGCCCA GGCAGGGGCA GATGTGGTGG ATGTGGCTGT GGACTCCATG 
TCGGGGATCA CGTCCCAGCC CAGCATGGGG GCCCTGGTGG CCTGCACTCT GGGGACCCCC 
TTCGACACAG GGCTACCTTT GGAGCGGGTG TTTGACTACA GCGAGTACTG GGAGGGGGCC 
CGGGGGCTAT ACGCCCCCTT CGACTGCACG GCCACCATGA AATCCGGGAA CTCGGATGTG 
TACGAGAACG AGATCCCGGG AGGCCAGTAC ACAAACCTGC ACTTCCAGCC TTACAGCATG 
GGGCTTGGCA CAAAGTTCAA AGAAGTCAAA AAGGCCTATG TGAGGGGCAA CCAGATGCTG 
GGGGACCTCA TCAAGGTGAC CCCTTCCTCC AAGATCGTGG GGGACCTGGC CCAGTTCATG 
GTGCAGAATG GGCTGAGCCG AGCCGACGTG GAGGCCAAGG CCGAGGAACT GTCCTTCCCG 
CGCTCGGTGG TGGAGTTCTT GCAGGGCTAC ATTGGCATAC CCTATGGGGG CTTCCCCGAA 
CCCTTTCGAT CCAAGGTCCT AAAGGACCTG CCCCGGGTCG AGGGGCGGCC GGGCGCTTCC 
ATGACCGCCC TGGACTTGAG GAAGCTGGAG GATGAGCTGA AGGAACGCAA CGACGAGATA 
ACCCCCGAGG ACGTGCTCTC GGCCGCCATG TATCCCGACG TCTTCCAGCA GTTCAAGGAC 
TTCACGGCCA CCTTCGGGCC CCTGGAGTGC CTCAACACCC GCCTCTTCCT GCAAGGGCCC 
AAGGTCGCCG AAGAGTTTGA GGTAGAGCTG GAACGGGGCA AAACCCTGCA CATCAAGGCC 
TTGGCCCTGG GGGACGTGAA CCGGGCCGGG CAGAGGCAGG TGTTCTTCGA GCTCAACGGG 
CAGGTGCGCT CCATCCTGGT CAAGGACACC CAGGCCATGA AGGAGATGCA CTTCCACCCC 
AAGGCCCTGA AGGACGTCAA GGGCCAGATC GGGGCCCCGA TGCCCGGCAA GGTGATCGAC 
ATCAAGGTGA AGGTGGGGGA CAAGGTGACC AAGGGGACGC CGCTGTGCGT GCTGAGCGCC 
ATGAAGATGG AGACCATGGT GACGGCGCCC CTGGAGGGCA CCATCGCCAA GGTCCACGTG 
ACTCGCGACA TGACTCTGGA GGGCGACGAC CTCATCTTGG AGATCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007502712.1
CDS	132..3662
Translation	MMLKFHLAQRGLRLLSIRHASMMPPSNAQHIKYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLSPVQAYLNIADIIKVAKENDVDAIHPGYGFLSERADFAQACLDAGIKFIGPSPEVVRKMGDKVRARPIPIAAGVPVVPGTDTPISSLHEAHEFSKTYGLPIIFKAAYGGGGRGMRVVQNFEELDENFNRAYSEALAAFGDGTLFVEKFIENPRHIEVQILGDQHGNIVHLYERDCSIQRRHQKVVEIAPATLLDTQLRNRLTSDSVKLGKQVGYENAGTVEFLIDKHGKHYFIEVNSRLQVEHTVTEEITHVDLVHAQIHVAEGRSLPDLGLLQENIRISGCAIQCRVTTEDPSRGFQPDTGRIEVFRSGEGMGIRLDNASAFQGSVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDSNPELFQMRPVQNRAQKLLHYLGHVMVNGPQTPIPVKASSSPTDPIIPPVPMGPPPPGFRDILLREGPEGFARAVRNHKGLLLMDTTFRDAHQSLLATRVRTYDLKKIAPYVAHSFNSLFSLENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYMPNLLLGMEAAGSAGGVVEAAISYTGDVADPTRTKYSLKYYMDLAEELVRAGTHILSIKDMAGLLKPAACTMLVGALRDRFPELPLHIHTHDTAGSGVANMLACAQAGADVVDVAVDSMSGITSQPSMGALVACTLGTPFDTGLPLERVFDYSEYWEGARGLYAPFDCTATMKSGNSDVYENEIPGGQYTNLHFQPYSMGLGTKFKEVKKAYVRGNQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRADVEAKAEELSFPRSVVEFLQGYIGIPYGGFPEPFRSKVLKDLPRVEGRPGASMTALDLRKLEDELKERNDEITPEDVLSAAMYPDVFQQFKDFTATFGPLECLNTRLFLQGPKVAEEFEVELERGKTLHIKALALGDVNRAGQRQVFFELNGQVRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVKVGDKVTKGTPLCVLSAMKMETMVTAPLEGTIAKVHVTRDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_007502650.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica pyruvate carboxylase (PC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007502650.2

ORF Insert Sequence:

ATGATGTTGA AGTTTCATTT GGCCCAAAGG GGCCTAAGGC TCCTAAGTAT CCGACATGCC 
TCCATGATGC CCCCCTCAAA CGCCCAACAC ATCAAGTACA AGCCCATCAA GAAAGTCATG 
GTGGCCAACC GAGGTGAGAT AGCCATTCGA GTGTTCCGGG CCTGCACGGA GCTTGGCATC 
CGCACCGTGG CCGTGTATTC CGAGCAGGAC ACTGGGCAGA TGCATCGGCA GAAGGCAGAT 
GAGGCTTACC TCATTGGCCG TGGTCTTAGC CCAGTGCAGG CCTACCTGAA CATCGCAGAC 
ATCATCAAGG TGGCCAAGGA GAATGATGTC GATGCCATTC ACCCCGGCTA TGGGTTCTTG 
TCGGAGCGTG CTGATTTTGC TCAGGCCTGT CTGGATGCTG GGATCAAATT CATTGGCCCC 
AGCCCTGAAG TGGTGCGCAA GATGGGGGAC AAGGTGAGGG CCCGGCCCAT TCCCATTGCT 
GCAGGTGTCC CAGTGGTTCC AGGCACAGAC ACCCCCATCT CCTCCTTACA CGAGGCTCAC 
GAATTCTCCA AAACCTATGG CCTCCCAATT ATCTTCAAAG CTGCCTATGG AGGCGGGGGT 
CGTGGCATGA GGGTCGTACA AAACTTTGAG GAATTGGATG AGAATTTTAA CCGGGCCTAT 
TCCGAGGCCC TGGCCGCCTT TGGGGATGGG ACTCTGTTTG TGGAAAAATT CATTGAGAAT 
CCACGCCACA TTGAAGTGCA AATTCTGGGG GACCAGCATG GGAACATTGT GCACCTCTAC 
GAACGGGACT GCTCGATCCA GCGGAGGCAT CAGAAGGTGG TGGAGATTGC TCCGGCCACT 
CTCCTGGACA CCCAGCTCCG GAATCGCCTG ACCAGTGATT CGGTCAAGTT AGGCAAGCAG 
GTGGGCTATG AGAACGCTGG CACAGTGGAG TTCCTCATAG ACAAACACGG CAAACACTAT 
TTCATCGAGG TCAATTCTCG CCTACAAGTG GAGCACACGG TCACCGAGGA GATCACCCAC 
GTAGACCTGG TCCATGCCCA GATCCATGTA GCTGAGGGCC GGAGCCTGCC GGATCTGGGC 
CTTCTGCAAG AGAATATTCG AATCAGCGGT TGTGCAATCC AGTGTCGGGT GACAACAGAG 
GACCCGTCTC GAGGCTTCCA GCCAGACACT GGCCGGATCG AGGTGTTCCG TAGTGGTGAG 
GGGATGGGCA TCCGCTTGGA CAACGCCTCT GCCTTCCAAG GGTCAGTCAT CTCCCCGCAC 
TACGATTCCC TGTTGGTCAA AGTCATAGCC CATGGAAAGG ACCACCCCAC GGCGGCCACC 
AAGATGAGCC GGGCACTAGC CGAGTTCCGG GTCCGAGGGG TCAAGACCAA CATCCCCTTC 
CTGCAGAATG TTCTCAACAA CCAGCAGTTC CTGGCGGGCA CCGTGGATAC CCAGTTCATC 
GATTCAAACC CTGAGCTCTT CCAGATGCGG CCTGTTCAGA ACCGGGCCCA GAAGCTGCTG 
CACTACCTGG GTCACGTCAT GGTGAATGGG CCCCAGACAC CGATCCCGGT CAAGGCAAGC 
TCCAGCCCCA CGGATCCCAT CATCCCCCCC GTCCCCATGG GCCCACCTCC TCCTGGTTTC 
AGAGACATCC TGCTGCGTGA GGGTCCCGAG GGCTTTGCCC GGGCCGTGCG GAACCACAAG 
GGGCTGCTGC TAATGGACAC GACCTTCCGG GATGCCCATC AGTCCCTGCT GGCCACACGA 
GTGAGGACCT ACGACCTCAA GAAGATCGCC CCTTATGTCG CCCACAGTTT CAACAGCCTT 
TTCAGTCTGG AGAACTGGGG GGGAGCCACG TTTGATGTTG CCATGCGCTT CCTGTACGAG 
TGTCCGTGGC GGAGGCTACA GGAGCTCAGA GAGCTCATCC CCAACATCCC CTTCCAGATG 
CTGCTCCGGG GTGCCAACGC TGTGGGCTAC ACCAACTACC CGGACAACGT GGTCTTTAAG 
TTCTGTGAGG TGGCCAAGGA GAACGGGATG GACGTGTTCC GTGTCTTTGA TTCCCTCAAC 
TACATGCCCA ATCTGCTGCT GGGCATGGAG GCGGCAGGCA GTGCCGGAGG TGTGGTGGAG 
GCAGCCATCT CCTACACGGG CGACGTGGCC GACCCCACGC GCACCAAGTA TTCCCTGAAG 
TACTACATGG ACCTGGCTGA GGAGCTGGTG CGGGCTGGGA CACACATCCT GAGCATCAAG 
GACATGGCAG GCCTTCTGAA GCCGGCTGCA TGCACCATGC TGGTGGGTGC CCTCCGAGAC 
CGCTTCCCAG AGCTCCCCCT GCACATCCAC ACCCATGACA CGGCCGGCTC AGGTGTGGCC 
AATATGCTGG CCTGTGCCCA GGCAGGGGCA GATGTGGTGG ATGTGGCTGT GGACTCCATG 
TCGGGGATCA CGTCCCAGCC CAGCATGGGG GCCCTGGTGG CCTGCACTCT GGGGACCCCC 
TTCGACACAG GGCTACCTTT GGAGCGGGTG TTTGACTACA GCGAGTACTG GGAGGGGGCC 
CGGGGGCTAT ACGCCCCCTT CGACTGCACG GCCACCATGA AATCCGGGAA CTCGGATGTG 
TACGAGAACG AGATCCCGGG AGGCCAGTAC ACAAACCTGC ACTTCCAGCC TTACAGCATG 
GGGCTTGGCA CAAAGTTCAA AGAAGTCAAA AAGGCCTATG TGAGGGGCAA CCAGATGCTG 
GGGGACCTCA TCAAGGTGAC CCCTTCCTCC AAGATCGTGG GGGACCTGGC CCAGTTCATG 
GTGCAGAATG GGCTGAGCCG AGCCGACGTG GAGGCCAAGG CCGAGGAACT GTCCTTCCCG 
CGCTCGGTGG TGGAGTTCTT GCAGGGCTAC ATTGGCATAC CCTATGGGGG CTTCCCCGAA 
CCCTTTCGAT CCAAGGTCCT AAAGGACCTG CCCCGGGTCG AGGGGCGGCC GGGCGCTTCC 
ATGACCGCCC TGGACTTGAG GAAGCTGGAG GATGAGCTGA AGGAACGCAA CGACGAGATA 
ACCCCCGAGG ACGTGCTCTC GGCCGCCATG TATCCCGACG TCTTCCAGCA GTTCAAGGAC 
TTCACGGCCA CCTTCGGGCC CCTGGAGTGC CTCAACACCC GCCTCTTCCT GCAAGGGCCC 
AAGGTCGCCG AAGAGTTTGA GGTAGAGCTG GAACGGGGCA AAACCCTGCA CATCAAGGCC 
TTGGCCCTGG GGGACGTGAA CCGGGCCGGG CAGAGGCAGG TGTTCTTCGA GCTCAACGGG 
CAGGTGCGCT CCATCCTGGT CAAGGACACC CAGGCCATGA AGGAGATGCA CTTCCACCCC 
AAGGCCCTGA AGGACGTCAA GGGCCAGATC GGGGCCCCGA TGCCCGGCAA GGTGATCGAC 
ATCAAGGTGA AGGTGGGGGA CAAGGTGACC AAGGGGACGC CGCTGTGCGT GCTGAGCGCC 
ATGAAGATGG AGACCATGGT GACGGCGCCC CTGGAGGGCA CCATCGCCAA GGTCCACGTG 
ACTCGCGACA TGACTCTGGA GGGCGACGAC CTCATCTTGG AGATCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_007502651.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007502713.1 Monodelphis domestica pyruvate carboxylase (PC), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMc31726
Clone ID Related Accession (Same CDS sequence)	XM_007502651.2 , XM_001364491.3 , XM_007502652.2
Accession Version	XM_007502651.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008808.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007502651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TTCATTTGGC CCAAAGGGGC CTAAGGCTCC TAAGTATCCG ACATGCCTCC 
ATGATGCCCC CCTCAAACGC CCAACACATC AAGTACAAGC CCATCAAGAA AGTCATGGTG 
GCCAACCGAG GTGAGATAGC CATTCGAGTG TTCCGGGCCT GCACGGAGCT TGGCATCCGC 
ACCGTGGCCG TGTATTCCGA GCAGGACACT GGGCAGATGC ATCGGCAGAA GGCAGATGAG 
GCTTACCTCA TTGGCCGTGG TCTTAGCCCA GTGCAGGCCT ACCTGAACAT CGCAGACATC 
ATCAAGGTGG CCAAGGAGAA TGATGTCGAT GCCATTCACC CCGGCTATGG GTTCTTGTCG 
GAGCGTGCTG ATTTTGCTCA GGCCTGTCTG GATGCTGGGA TCAAATTCAT TGGCCCCAGC 
CCTGAAGTGG TGCGCAAGAT GGGGGACAAG GTGAGGGCCC GGCCCATTCC CATTGCTGCA 
GGTGTCCCAG TGGTTCCAGG CACAGACACC CCCATCTCCT CCTTACACGA GGCTCACGAA 
TTCTCCAAAA CCTATGGCCT CCCAATTATC TTCAAAGCTG CCTATGGAGG CGGGGGTCGT 
GGCATGAGGG TCGTACAAAA CTTTGAGGAA TTGGATGAGA ATTTTAACCG GGCCTATTCC 
GAGGCCCTGG CCGCCTTTGG GGATGGGACT CTGTTTGTGG AAAAATTCAT TGAGAATCCA 
CGCCACATTG AAGTGCAAAT TCTGGGGGAC CAGCATGGGA ACATTGTGCA CCTCTACGAA 
CGGGACTGCT CGATCCAGCG GAGGCATCAG AAGGTGGTGG AGATTGCTCC GGCCACTCTC 
CTGGACACCC AGCTCCGGAA TCGCCTGACC AGTGATTCGG TCAAGTTAGG CAAGCAGGTG 
GGCTATGAGA ACGCTGGCAC AGTGGAGTTC CTCATAGACA AACACGGCAA ACACTATTTC 
ATCGAGGTCA ATTCTCGCCT ACAAGTGGAG CACACGGTCA CCGAGGAGAT CACCCACGTA 
GACCTGGTCC ATGCCCAGAT CCATGTAGCT GAGGGCCGGA GCCTGCCGGA TCTGGGCCTT 
CTGCAAGAGA ATATTCGAAT CAGCGGTTGT GCAATCCAGT GTCGGGTGAC AACAGAGGAC 
CCGTCTCGAG GCTTCCAGCC AGACACTGGC CGGATCGAGG TGTTCCGTAG TGGTGAGGGG 
ATGGGCATCC GCTTGGACAA CGCCTCTGCC TTCCAAGGGT CAGTCATCTC CCCGCACTAC 
GATTCCCTGT TGGTCAAAGT CATAGCCCAT GGAAAGGACC ACCCCACGGC GGCCACCAAG 
ATGAGCCGGG CACTAGCCGA GTTCCGGGTC CGAGGGGTCA AGACCAACAT CCCCTTCCTG 
CAGAATGTTC TCAACAACCA GCAGTTCCTG GCGGGCACCG TGGATACCCA GTTCATCGAT 
TCAAACCCTG AGCTCTTCCA GATGCGGCCT GTTCAGAACC GGGCCCAGAA GCTGCTGCAC 
TACCTGGGTC ACGTCATGGT GAATGGGCCC CAGACACCGA TCCCGGTCAA GGCAAGCTCC 
AGCCCCACGG ATCCCATCAT CCCCCCCGTC CCCATGGGCC CACCTCCTCC TGGTTTCAGA 
GACATCCTGC TGCGTGAGGG TCCCGAGGGC TTTGCCCGGG CCGTGCGGAA CCACAAGGGG 
CTGCTGCTAA TGGACACGAC CTTCCGGGAT GCCCATCAGT CCCTGCTGGC CACACGAGTG 
AGGACCTACG ACCTCAAGAA GATCGCCCCT TATGTCGCCC ACAGTTTCAA CAGCCTTTTC 
AGTCTGGAGA ACTGGGGGGG AGCCACGTTT GATGTTGCCA TGCGCTTCCT GTACGAGTGT 
CCGTGGCGGA GGCTACAGGA GCTCAGAGAG CTCATCCCCA ACATCCCCTT CCAGATGCTG 
CTCCGGGGTG CCAACGCTGT GGGCTACACC AACTACCCGG ACAACGTGGT CTTTAAGTTC 
TGTGAGGTGG CCAAGGAGAA CGGGATGGAC GTGTTCCGTG TCTTTGATTC CCTCAACTAC 
ATGCCCAATC TGCTGCTGGG CATGGAGGCG GCAGGCAGTG CCGGAGGTGT GGTGGAGGCA 
GCCATCTCCT ACACGGGCGA CGTGGCCGAC CCCACGCGCA CCAAGTATTC CCTGAAGTAC 
TACATGGACC TGGCTGAGGA GCTGGTGCGG GCTGGGACAC ACATCCTGAG CATCAAGGAC 
ATGGCAGGCC TTCTGAAGCC GGCTGCATGC ACCATGCTGG TGGGTGCCCT CCGAGACCGC 
TTCCCAGAGC TCCCCCTGCA CATCCACACC CATGACACGG CCGGCTCAGG TGTGGCCAAT 
ATGCTGGCCT GTGCCCAGGC AGGGGCAGAT GTGGTGGATG TGGCTGTGGA CTCCATGTCG 
GGGATCACGT CCCAGCCCAG CATGGGGGCC CTGGTGGCCT GCACTCTGGG GACCCCCTTC 
GACACAGGGC TACCTTTGGA GCGGGTGTTT GACTACAGCG AGTACTGGGA GGGGGCCCGG 
GGGCTATACG CCCCCTTCGA CTGCACGGCC ACCATGAAAT CCGGGAACTC GGATGTGTAC 
GAGAACGAGA TCCCGGGAGG CCAGTACACA AACCTGCACT TCCAGCCTTA CAGCATGGGG 
CTTGGCACAA AGTTCAAAGA AGTCAAAAAG GCCTATGTGA GGGGCAACCA GATGCTGGGG 
GACCTCATCA AGGTGACCCC TTCCTCCAAG ATCGTGGGGG ACCTGGCCCA GTTCATGGTG 
CAGAATGGGC TGAGCCGAGC CGACGTGGAG GCCAAGGCCG AGGAACTGTC CTTCCCGCGC 
TCGGTGGTGG AGTTCTTGCA GGGCTACATT GGCATACCCT ATGGGGGCTT CCCCGAACCC 
TTTCGATCCA AGGTCCTAAA GGACCTGCCC CGGGTCGAGG GGCGGCCGGG CGCTTCCATG 
ACCGCCCTGG ACTTGAGGAA GCTGGAGGAT GAGCTGAAGG AACGCAACGA CGAGATAACC 
CCCGAGGACG TGCTCTCGGC CGCCATGTAT CCCGACGTCT TCCAGCAGTT CAAGGACTTC 
ACGGCCACCT TCGGGCCCCT GGAGTGCCTC AACACCCGCC TCTTCCTGCA AGGGCCCAAG 
GTCGCCGAAG AGTTTGAGGT AGAGCTGGAA CGGGGCAAAA CCCTGCACAT CAAGGCCTTG 
GCCCTGGGGG ACGTGAACCG GGCCGGGCAG AGGCAGGTGT TCTTCGAGCT CAACGGGCAG 
GTGCGCTCCA TCCTGGTCAA GGACACCCAG GCCATGAAGG AGATGCACTT CCACCCCAAG 
GCCCTGAAGG ACGTCAAGGG CCAGATCGGG GCCCCGATGC CCGGCAAGGT GATCGACATC 
AAGGTGAAGG TGGGGGACAA GGTGACCAAG GGGACGCCGC TGTGCGTGCT GAGCGCCATG 
AAGATGGAGA CCATGGTGAC GGCGCCCCTG GAGGGCACCA TCGCCAAGGT CCACGTGACT 
CGCGACATGA CTCTGGAGGG CGACGACCTC ATCTTGGAGA TCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007502713.1
CDS	427..3954
Translation	MLKFHLAQRGLRLLSIRHASMMPPSNAQHIKYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLSPVQAYLNIADIIKVAKENDVDAIHPGYGFLSERADFAQACLDAGIKFIGPSPEVVRKMGDKVRARPIPIAAGVPVVPGTDTPISSLHEAHEFSKTYGLPIIFKAAYGGGGRGMRVVQNFEELDENFNRAYSEALAAFGDGTLFVEKFIENPRHIEVQILGDQHGNIVHLYERDCSIQRRHQKVVEIAPATLLDTQLRNRLTSDSVKLGKQVGYENAGTVEFLIDKHGKHYFIEVNSRLQVEHTVTEEITHVDLVHAQIHVAEGRSLPDLGLLQENIRISGCAIQCRVTTEDPSRGFQPDTGRIEVFRSGEGMGIRLDNASAFQGSVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDSNPELFQMRPVQNRAQKLLHYLGHVMVNGPQTPIPVKASSSPTDPIIPPVPMGPPPPGFRDILLREGPEGFARAVRNHKGLLLMDTTFRDAHQSLLATRVRTYDLKKIAPYVAHSFNSLFSLENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYMPNLLLGMEAAGSAGGVVEAAISYTGDVADPTRTKYSLKYYMDLAEELVRAGTHILSIKDMAGLLKPAACTMLVGALRDRFPELPLHIHTHDTAGSGVANMLACAQAGADVVDVAVDSMSGITSQPSMGALVACTLGTPFDTGLPLERVFDYSEYWEGARGLYAPFDCTATMKSGNSDVYENEIPGGQYTNLHFQPYSMGLGTKFKEVKKAYVRGNQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRADVEAKAEELSFPRSVVEFLQGYIGIPYGGFPEPFRSKVLKDLPRVEGRPGASMTALDLRKLEDELKERNDEITPEDVLSAAMYPDVFQQFKDFTATFGPLECLNTRLFLQGPKVAEEFEVELERGKTLHIKALALGDVNRAGQRQVFFELNGQVRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVKVGDKVTKGTPLCVLSAMKMETMVTAPLEGTIAKVHVTRDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_007502651.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica pyruvate carboxylase (PC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007502651.2

ORF Insert Sequence:

ATGTTGAAGT TTCATTTGGC CCAAAGGGGC CTAAGGCTCC TAAGTATCCG ACATGCCTCC 
ATGATGCCCC CCTCAAACGC CCAACACATC AAGTACAAGC CCATCAAGAA AGTCATGGTG 
GCCAACCGAG GTGAGATAGC CATTCGAGTG TTCCGGGCCT GCACGGAGCT TGGCATCCGC 
ACCGTGGCCG TGTATTCCGA GCAGGACACT GGGCAGATGC ATCGGCAGAA GGCAGATGAG 
GCTTACCTCA TTGGCCGTGG TCTTAGCCCA GTGCAGGCCT ACCTGAACAT CGCAGACATC 
ATCAAGGTGG CCAAGGAGAA TGATGTCGAT GCCATTCACC CCGGCTATGG GTTCTTGTCG 
GAGCGTGCTG ATTTTGCTCA GGCCTGTCTG GATGCTGGGA TCAAATTCAT TGGCCCCAGC 
CCTGAAGTGG TGCGCAAGAT GGGGGACAAG GTGAGGGCCC GGCCCATTCC CATTGCTGCA 
GGTGTCCCAG TGGTTCCAGG CACAGACACC CCCATCTCCT CCTTACACGA GGCTCACGAA 
TTCTCCAAAA CCTATGGCCT CCCAATTATC TTCAAAGCTG CCTATGGAGG CGGGGGTCGT 
GGCATGAGGG TCGTACAAAA CTTTGAGGAA TTGGATGAGA ATTTTAACCG GGCCTATTCC 
GAGGCCCTGG CCGCCTTTGG GGATGGGACT CTGTTTGTGG AAAAATTCAT TGAGAATCCA 
CGCCACATTG AAGTGCAAAT TCTGGGGGAC CAGCATGGGA ACATTGTGCA CCTCTACGAA 
CGGGACTGCT CGATCCAGCG GAGGCATCAG AAGGTGGTGG AGATTGCTCC GGCCACTCTC 
CTGGACACCC AGCTCCGGAA TCGCCTGACC AGTGATTCGG TCAAGTTAGG CAAGCAGGTG 
GGCTATGAGA ACGCTGGCAC AGTGGAGTTC CTCATAGACA AACACGGCAA ACACTATTTC 
ATCGAGGTCA ATTCTCGCCT ACAAGTGGAG CACACGGTCA CCGAGGAGAT CACCCACGTA 
GACCTGGTCC ATGCCCAGAT CCATGTAGCT GAGGGCCGGA GCCTGCCGGA TCTGGGCCTT 
CTGCAAGAGA ATATTCGAAT CAGCGGTTGT GCAATCCAGT GTCGGGTGAC AACAGAGGAC 
CCGTCTCGAG GCTTCCAGCC AGACACTGGC CGGATCGAGG TGTTCCGTAG TGGTGAGGGG 
ATGGGCATCC GCTTGGACAA CGCCTCTGCC TTCCAAGGGT CAGTCATCTC CCCGCACTAC 
GATTCCCTGT TGGTCAAAGT CATAGCCCAT GGAAAGGACC ACCCCACGGC GGCCACCAAG 
ATGAGCCGGG CACTAGCCGA GTTCCGGGTC CGAGGGGTCA AGACCAACAT CCCCTTCCTG 
CAGAATGTTC TCAACAACCA GCAGTTCCTG GCGGGCACCG TGGATACCCA GTTCATCGAT 
TCAAACCCTG AGCTCTTCCA GATGCGGCCT GTTCAGAACC GGGCCCAGAA GCTGCTGCAC 
TACCTGGGTC ACGTCATGGT GAATGGGCCC CAGACACCGA TCCCGGTCAA GGCAAGCTCC 
AGCCCCACGG ATCCCATCAT CCCCCCCGTC CCCATGGGCC CACCTCCTCC TGGTTTCAGA 
GACATCCTGC TGCGTGAGGG TCCCGAGGGC TTTGCCCGGG CCGTGCGGAA CCACAAGGGG 
CTGCTGCTAA TGGACACGAC CTTCCGGGAT GCCCATCAGT CCCTGCTGGC CACACGAGTG 
AGGACCTACG ACCTCAAGAA GATCGCCCCT TATGTCGCCC ACAGTTTCAA CAGCCTTTTC 
AGTCTGGAGA ACTGGGGGGG AGCCACGTTT GATGTTGCCA TGCGCTTCCT GTACGAGTGT 
CCGTGGCGGA GGCTACAGGA GCTCAGAGAG CTCATCCCCA ACATCCCCTT CCAGATGCTG 
CTCCGGGGTG CCAACGCTGT GGGCTACACC AACTACCCGG ACAACGTGGT CTTTAAGTTC 
TGTGAGGTGG CCAAGGAGAA CGGGATGGAC GTGTTCCGTG TCTTTGATTC CCTCAACTAC 
ATGCCCAATC TGCTGCTGGG CATGGAGGCG GCAGGCAGTG CCGGAGGTGT GGTGGAGGCA 
GCCATCTCCT ACACGGGCGA CGTGGCCGAC CCCACGCGCA CCAAGTATTC CCTGAAGTAC 
TACATGGACC TGGCTGAGGA GCTGGTGCGG GCTGGGACAC ACATCCTGAG CATCAAGGAC 
ATGGCAGGCC TTCTGAAGCC GGCTGCATGC ACCATGCTGG TGGGTGCCCT CCGAGACCGC 
TTCCCAGAGC TCCCCCTGCA CATCCACACC CATGACACGG CCGGCTCAGG TGTGGCCAAT 
ATGCTGGCCT GTGCCCAGGC AGGGGCAGAT GTGGTGGATG TGGCTGTGGA CTCCATGTCG 
GGGATCACGT CCCAGCCCAG CATGGGGGCC CTGGTGGCCT GCACTCTGGG GACCCCCTTC 
GACACAGGGC TACCTTTGGA GCGGGTGTTT GACTACAGCG AGTACTGGGA GGGGGCCCGG 
GGGCTATACG CCCCCTTCGA CTGCACGGCC ACCATGAAAT CCGGGAACTC GGATGTGTAC 
GAGAACGAGA TCCCGGGAGG CCAGTACACA AACCTGCACT TCCAGCCTTA CAGCATGGGG 
CTTGGCACAA AGTTCAAAGA AGTCAAAAAG GCCTATGTGA GGGGCAACCA GATGCTGGGG 
GACCTCATCA AGGTGACCCC TTCCTCCAAG ATCGTGGGGG ACCTGGCCCA GTTCATGGTG 
CAGAATGGGC TGAGCCGAGC CGACGTGGAG GCCAAGGCCG AGGAACTGTC CTTCCCGCGC 
TCGGTGGTGG AGTTCTTGCA GGGCTACATT GGCATACCCT ATGGGGGCTT CCCCGAACCC 
TTTCGATCCA AGGTCCTAAA GGACCTGCCC CGGGTCGAGG GGCGGCCGGG CGCTTCCATG 
ACCGCCCTGG ACTTGAGGAA GCTGGAGGAT GAGCTGAAGG AACGCAACGA CGAGATAACC 
CCCGAGGACG TGCTCTCGGC CGCCATGTAT CCCGACGTCT TCCAGCAGTT CAAGGACTTC 
ACGGCCACCT TCGGGCCCCT GGAGTGCCTC AACACCCGCC TCTTCCTGCA AGGGCCCAAG 
GTCGCCGAAG AGTTTGAGGT AGAGCTGGAA CGGGGCAAAA CCCTGCACAT CAAGGCCTTG 
GCCCTGGGGG ACGTGAACCG GGCCGGGCAG AGGCAGGTGT TCTTCGAGCT CAACGGGCAG 
GTGCGCTCCA TCCTGGTCAA GGACACCCAG GCCATGAAGG AGATGCACTT CCACCCCAAG 
GCCCTGAAGG ACGTCAAGGG CCAGATCGGG GCCCCGATGC CCGGCAAGGT GATCGACATC 
AAGGTGAAGG TGGGGGACAA GGTGACCAAG GGGACGCCGC TGTGCGTGCT GAGCGCCATG 
AAGATGGAGA CCATGGTGAC GGCGCCCCTG GAGGGCACCA TCGCCAAGGT CCACGTGACT 
CGCGACATGA CTCTGGAGGG CGACGACCTC ATCTTGGAGA TCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_001364491.3 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_001364528.1 Monodelphis domestica pyruvate carboxylase (PC), transcript variant X4, mRNA.

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CloneID	OMc31726
Clone ID Related Accession (Same CDS sequence)	XM_007502651.2 , XM_001364491.3 , XM_007502652.2
Accession Version	XM_001364491.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008808.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_001364491.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TTCATTTGGC CCAAAGGGGC CTAAGGCTCC TAAGTATCCG ACATGCCTCC 
ATGATGCCCC CCTCAAACGC CCAACACATC AAGTACAAGC CCATCAAGAA AGTCATGGTG 
GCCAACCGAG GTGAGATAGC CATTCGAGTG TTCCGGGCCT GCACGGAGCT TGGCATCCGC 
ACCGTGGCCG TGTATTCCGA GCAGGACACT GGGCAGATGC ATCGGCAGAA GGCAGATGAG 
GCTTACCTCA TTGGCCGTGG TCTTAGCCCA GTGCAGGCCT ACCTGAACAT CGCAGACATC 
ATCAAGGTGG CCAAGGAGAA TGATGTCGAT GCCATTCACC CCGGCTATGG GTTCTTGTCG 
GAGCGTGCTG ATTTTGCTCA GGCCTGTCTG GATGCTGGGA TCAAATTCAT TGGCCCCAGC 
CCTGAAGTGG TGCGCAAGAT GGGGGACAAG GTGAGGGCCC GGCCCATTCC CATTGCTGCA 
GGTGTCCCAG TGGTTCCAGG CACAGACACC CCCATCTCCT CCTTACACGA GGCTCACGAA 
TTCTCCAAAA CCTATGGCCT CCCAATTATC TTCAAAGCTG CCTATGGAGG CGGGGGTCGT 
GGCATGAGGG TCGTACAAAA CTTTGAGGAA TTGGATGAGA ATTTTAACCG GGCCTATTCC 
GAGGCCCTGG CCGCCTTTGG GGATGGGACT CTGTTTGTGG AAAAATTCAT TGAGAATCCA 
CGCCACATTG AAGTGCAAAT TCTGGGGGAC CAGCATGGGA ACATTGTGCA CCTCTACGAA 
CGGGACTGCT CGATCCAGCG GAGGCATCAG AAGGTGGTGG AGATTGCTCC GGCCACTCTC 
CTGGACACCC AGCTCCGGAA TCGCCTGACC AGTGATTCGG TCAAGTTAGG CAAGCAGGTG 
GGCTATGAGA ACGCTGGCAC AGTGGAGTTC CTCATAGACA AACACGGCAA ACACTATTTC 
ATCGAGGTCA ATTCTCGCCT ACAAGTGGAG CACACGGTCA CCGAGGAGAT CACCCACGTA 
GACCTGGTCC ATGCCCAGAT CCATGTAGCT GAGGGCCGGA GCCTGCCGGA TCTGGGCCTT 
CTGCAAGAGA ATATTCGAAT CAGCGGTTGT GCAATCCAGT GTCGGGTGAC AACAGAGGAC 
CCGTCTCGAG GCTTCCAGCC AGACACTGGC CGGATCGAGG TGTTCCGTAG TGGTGAGGGG 
ATGGGCATCC GCTTGGACAA CGCCTCTGCC TTCCAAGGGT CAGTCATCTC CCCGCACTAC 
GATTCCCTGT TGGTCAAAGT CATAGCCCAT GGAAAGGACC ACCCCACGGC GGCCACCAAG 
ATGAGCCGGG CACTAGCCGA GTTCCGGGTC CGAGGGGTCA AGACCAACAT CCCCTTCCTG 
CAGAATGTTC TCAACAACCA GCAGTTCCTG GCGGGCACCG TGGATACCCA GTTCATCGAT 
TCAAACCCTG AGCTCTTCCA GATGCGGCCT GTTCAGAACC GGGCCCAGAA GCTGCTGCAC 
TACCTGGGTC ACGTCATGGT GAATGGGCCC CAGACACCGA TCCCGGTCAA GGCAAGCTCC 
AGCCCCACGG ATCCCATCAT CCCCCCCGTC CCCATGGGCC CACCTCCTCC TGGTTTCAGA 
GACATCCTGC TGCGTGAGGG TCCCGAGGGC TTTGCCCGGG CCGTGCGGAA CCACAAGGGG 
CTGCTGCTAA TGGACACGAC CTTCCGGGAT GCCCATCAGT CCCTGCTGGC CACACGAGTG 
AGGACCTACG ACCTCAAGAA GATCGCCCCT TATGTCGCCC ACAGTTTCAA CAGCCTTTTC 
AGTCTGGAGA ACTGGGGGGG AGCCACGTTT GATGTTGCCA TGCGCTTCCT GTACGAGTGT 
CCGTGGCGGA GGCTACAGGA GCTCAGAGAG CTCATCCCCA ACATCCCCTT CCAGATGCTG 
CTCCGGGGTG CCAACGCTGT GGGCTACACC AACTACCCGG ACAACGTGGT CTTTAAGTTC 
TGTGAGGTGG CCAAGGAGAA CGGGATGGAC GTGTTCCGTG TCTTTGATTC CCTCAACTAC 
ATGCCCAATC TGCTGCTGGG CATGGAGGCG GCAGGCAGTG CCGGAGGTGT GGTGGAGGCA 
GCCATCTCCT ACACGGGCGA CGTGGCCGAC CCCACGCGCA CCAAGTATTC CCTGAAGTAC 
TACATGGACC TGGCTGAGGA GCTGGTGCGG GCTGGGACAC ACATCCTGAG CATCAAGGAC 
ATGGCAGGCC TTCTGAAGCC GGCTGCATGC ACCATGCTGG TGGGTGCCCT CCGAGACCGC 
TTCCCAGAGC TCCCCCTGCA CATCCACACC CATGACACGG CCGGCTCAGG TGTGGCCAAT 
ATGCTGGCCT GTGCCCAGGC AGGGGCAGAT GTGGTGGATG TGGCTGTGGA CTCCATGTCG 
GGGATCACGT CCCAGCCCAG CATGGGGGCC CTGGTGGCCT GCACTCTGGG GACCCCCTTC 
GACACAGGGC TACCTTTGGA GCGGGTGTTT GACTACAGCG AGTACTGGGA GGGGGCCCGG 
GGGCTATACG CCCCCTTCGA CTGCACGGCC ACCATGAAAT CCGGGAACTC GGATGTGTAC 
GAGAACGAGA TCCCGGGAGG CCAGTACACA AACCTGCACT TCCAGCCTTA CAGCATGGGG 
CTTGGCACAA AGTTCAAAGA AGTCAAAAAG GCCTATGTGA GGGGCAACCA GATGCTGGGG 
GACCTCATCA AGGTGACCCC TTCCTCCAAG ATCGTGGGGG ACCTGGCCCA GTTCATGGTG 
CAGAATGGGC TGAGCCGAGC CGACGTGGAG GCCAAGGCCG AGGAACTGTC CTTCCCGCGC 
TCGGTGGTGG AGTTCTTGCA GGGCTACATT GGCATACCCT ATGGGGGCTT CCCCGAACCC 
TTTCGATCCA AGGTCCTAAA GGACCTGCCC CGGGTCGAGG GGCGGCCGGG CGCTTCCATG 
ACCGCCCTGG ACTTGAGGAA GCTGGAGGAT GAGCTGAAGG AACGCAACGA CGAGATAACC 
CCCGAGGACG TGCTCTCGGC CGCCATGTAT CCCGACGTCT TCCAGCAGTT CAAGGACTTC 
ACGGCCACCT TCGGGCCCCT GGAGTGCCTC AACACCCGCC TCTTCCTGCA AGGGCCCAAG 
GTCGCCGAAG AGTTTGAGGT AGAGCTGGAA CGGGGCAAAA CCCTGCACAT CAAGGCCTTG 
GCCCTGGGGG ACGTGAACCG GGCCGGGCAG AGGCAGGTGT TCTTCGAGCT CAACGGGCAG 
GTGCGCTCCA TCCTGGTCAA GGACACCCAG GCCATGAAGG AGATGCACTT CCACCCCAAG 
GCCCTGAAGG ACGTCAAGGG CCAGATCGGG GCCCCGATGC CCGGCAAGGT GATCGACATC 
AAGGTGAAGG TGGGGGACAA GGTGACCAAG GGGACGCCGC TGTGCGTGCT GAGCGCCATG 
AAGATGGAGA CCATGGTGAC GGCGCCCCTG GAGGGCACCA TCGCCAAGGT CCACGTGACT 
CGCGACATGA CTCTGGAGGG CGACGACCTC ATCTTGGAGA TCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001364528.1
CDS	83..3610
Translation	MLKFHLAQRGLRLLSIRHASMMPPSNAQHIKYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLSPVQAYLNIADIIKVAKENDVDAIHPGYGFLSERADFAQACLDAGIKFIGPSPEVVRKMGDKVRARPIPIAAGVPVVPGTDTPISSLHEAHEFSKTYGLPIIFKAAYGGGGRGMRVVQNFEELDENFNRAYSEALAAFGDGTLFVEKFIENPRHIEVQILGDQHGNIVHLYERDCSIQRRHQKVVEIAPATLLDTQLRNRLTSDSVKLGKQVGYENAGTVEFLIDKHGKHYFIEVNSRLQVEHTVTEEITHVDLVHAQIHVAEGRSLPDLGLLQENIRISGCAIQCRVTTEDPSRGFQPDTGRIEVFRSGEGMGIRLDNASAFQGSVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDSNPELFQMRPVQNRAQKLLHYLGHVMVNGPQTPIPVKASSSPTDPIIPPVPMGPPPPGFRDILLREGPEGFARAVRNHKGLLLMDTTFRDAHQSLLATRVRTYDLKKIAPYVAHSFNSLFSLENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYMPNLLLGMEAAGSAGGVVEAAISYTGDVADPTRTKYSLKYYMDLAEELVRAGTHILSIKDMAGLLKPAACTMLVGALRDRFPELPLHIHTHDTAGSGVANMLACAQAGADVVDVAVDSMSGITSQPSMGALVACTLGTPFDTGLPLERVFDYSEYWEGARGLYAPFDCTATMKSGNSDVYENEIPGGQYTNLHFQPYSMGLGTKFKEVKKAYVRGNQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRADVEAKAEELSFPRSVVEFLQGYIGIPYGGFPEPFRSKVLKDLPRVEGRPGASMTALDLRKLEDELKERNDEITPEDVLSAAMYPDVFQQFKDFTATFGPLECLNTRLFLQGPKVAEEFEVELERGKTLHIKALALGDVNRAGQRQVFFELNGQVRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVKVGDKVTKGTPLCVLSAMKMETMVTAPLEGTIAKVHVTRDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_001364491.3
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica pyruvate carboxylase (PC), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001364491.3

ORF Insert Sequence:

ATGTTGAAGT TTCATTTGGC CCAAAGGGGC CTAAGGCTCC TAAGTATCCG ACATGCCTCC 
ATGATGCCCC CCTCAAACGC CCAACACATC AAGTACAAGC CCATCAAGAA AGTCATGGTG 
GCCAACCGAG GTGAGATAGC CATTCGAGTG TTCCGGGCCT GCACGGAGCT TGGCATCCGC 
ACCGTGGCCG TGTATTCCGA GCAGGACACT GGGCAGATGC ATCGGCAGAA GGCAGATGAG 
GCTTACCTCA TTGGCCGTGG TCTTAGCCCA GTGCAGGCCT ACCTGAACAT CGCAGACATC 
ATCAAGGTGG CCAAGGAGAA TGATGTCGAT GCCATTCACC CCGGCTATGG GTTCTTGTCG 
GAGCGTGCTG ATTTTGCTCA GGCCTGTCTG GATGCTGGGA TCAAATTCAT TGGCCCCAGC 
CCTGAAGTGG TGCGCAAGAT GGGGGACAAG GTGAGGGCCC GGCCCATTCC CATTGCTGCA 
GGTGTCCCAG TGGTTCCAGG CACAGACACC CCCATCTCCT CCTTACACGA GGCTCACGAA 
TTCTCCAAAA CCTATGGCCT CCCAATTATC TTCAAAGCTG CCTATGGAGG CGGGGGTCGT 
GGCATGAGGG TCGTACAAAA CTTTGAGGAA TTGGATGAGA ATTTTAACCG GGCCTATTCC 
GAGGCCCTGG CCGCCTTTGG GGATGGGACT CTGTTTGTGG AAAAATTCAT TGAGAATCCA 
CGCCACATTG AAGTGCAAAT TCTGGGGGAC CAGCATGGGA ACATTGTGCA CCTCTACGAA 
CGGGACTGCT CGATCCAGCG GAGGCATCAG AAGGTGGTGG AGATTGCTCC GGCCACTCTC 
CTGGACACCC AGCTCCGGAA TCGCCTGACC AGTGATTCGG TCAAGTTAGG CAAGCAGGTG 
GGCTATGAGA ACGCTGGCAC AGTGGAGTTC CTCATAGACA AACACGGCAA ACACTATTTC 
ATCGAGGTCA ATTCTCGCCT ACAAGTGGAG CACACGGTCA CCGAGGAGAT CACCCACGTA 
GACCTGGTCC ATGCCCAGAT CCATGTAGCT GAGGGCCGGA GCCTGCCGGA TCTGGGCCTT 
CTGCAAGAGA ATATTCGAAT CAGCGGTTGT GCAATCCAGT GTCGGGTGAC AACAGAGGAC 
CCGTCTCGAG GCTTCCAGCC AGACACTGGC CGGATCGAGG TGTTCCGTAG TGGTGAGGGG 
ATGGGCATCC GCTTGGACAA CGCCTCTGCC TTCCAAGGGT CAGTCATCTC CCCGCACTAC 
GATTCCCTGT TGGTCAAAGT CATAGCCCAT GGAAAGGACC ACCCCACGGC GGCCACCAAG 
ATGAGCCGGG CACTAGCCGA GTTCCGGGTC CGAGGGGTCA AGACCAACAT CCCCTTCCTG 
CAGAATGTTC TCAACAACCA GCAGTTCCTG GCGGGCACCG TGGATACCCA GTTCATCGAT 
TCAAACCCTG AGCTCTTCCA GATGCGGCCT GTTCAGAACC GGGCCCAGAA GCTGCTGCAC 
TACCTGGGTC ACGTCATGGT GAATGGGCCC CAGACACCGA TCCCGGTCAA GGCAAGCTCC 
AGCCCCACGG ATCCCATCAT CCCCCCCGTC CCCATGGGCC CACCTCCTCC TGGTTTCAGA 
GACATCCTGC TGCGTGAGGG TCCCGAGGGC TTTGCCCGGG CCGTGCGGAA CCACAAGGGG 
CTGCTGCTAA TGGACACGAC CTTCCGGGAT GCCCATCAGT CCCTGCTGGC CACACGAGTG 
AGGACCTACG ACCTCAAGAA GATCGCCCCT TATGTCGCCC ACAGTTTCAA CAGCCTTTTC 
AGTCTGGAGA ACTGGGGGGG AGCCACGTTT GATGTTGCCA TGCGCTTCCT GTACGAGTGT 
CCGTGGCGGA GGCTACAGGA GCTCAGAGAG CTCATCCCCA ACATCCCCTT CCAGATGCTG 
CTCCGGGGTG CCAACGCTGT GGGCTACACC AACTACCCGG ACAACGTGGT CTTTAAGTTC 
TGTGAGGTGG CCAAGGAGAA CGGGATGGAC GTGTTCCGTG TCTTTGATTC CCTCAACTAC 
ATGCCCAATC TGCTGCTGGG CATGGAGGCG GCAGGCAGTG CCGGAGGTGT GGTGGAGGCA 
GCCATCTCCT ACACGGGCGA CGTGGCCGAC CCCACGCGCA CCAAGTATTC CCTGAAGTAC 
TACATGGACC TGGCTGAGGA GCTGGTGCGG GCTGGGACAC ACATCCTGAG CATCAAGGAC 
ATGGCAGGCC TTCTGAAGCC GGCTGCATGC ACCATGCTGG TGGGTGCCCT CCGAGACCGC 
TTCCCAGAGC TCCCCCTGCA CATCCACACC CATGACACGG CCGGCTCAGG TGTGGCCAAT 
ATGCTGGCCT GTGCCCAGGC AGGGGCAGAT GTGGTGGATG TGGCTGTGGA CTCCATGTCG 
GGGATCACGT CCCAGCCCAG CATGGGGGCC CTGGTGGCCT GCACTCTGGG GACCCCCTTC 
GACACAGGGC TACCTTTGGA GCGGGTGTTT GACTACAGCG AGTACTGGGA GGGGGCCCGG 
GGGCTATACG CCCCCTTCGA CTGCACGGCC ACCATGAAAT CCGGGAACTC GGATGTGTAC 
GAGAACGAGA TCCCGGGAGG CCAGTACACA AACCTGCACT TCCAGCCTTA CAGCATGGGG 
CTTGGCACAA AGTTCAAAGA AGTCAAAAAG GCCTATGTGA GGGGCAACCA GATGCTGGGG 
GACCTCATCA AGGTGACCCC TTCCTCCAAG ATCGTGGGGG ACCTGGCCCA GTTCATGGTG 
CAGAATGGGC TGAGCCGAGC CGACGTGGAG GCCAAGGCCG AGGAACTGTC CTTCCCGCGC 
TCGGTGGTGG AGTTCTTGCA GGGCTACATT GGCATACCCT ATGGGGGCTT CCCCGAACCC 
TTTCGATCCA AGGTCCTAAA GGACCTGCCC CGGGTCGAGG GGCGGCCGGG CGCTTCCATG 
ACCGCCCTGG ACTTGAGGAA GCTGGAGGAT GAGCTGAAGG AACGCAACGA CGAGATAACC 
CCCGAGGACG TGCTCTCGGC CGCCATGTAT CCCGACGTCT TCCAGCAGTT CAAGGACTTC 
ACGGCCACCT TCGGGCCCCT GGAGTGCCTC AACACCCGCC TCTTCCTGCA AGGGCCCAAG 
GTCGCCGAAG AGTTTGAGGT AGAGCTGGAA CGGGGCAAAA CCCTGCACAT CAAGGCCTTG 
GCCCTGGGGG ACGTGAACCG GGCCGGGCAG AGGCAGGTGT TCTTCGAGCT CAACGGGCAG 
GTGCGCTCCA TCCTGGTCAA GGACACCCAG GCCATGAAGG AGATGCACTT CCACCCCAAG 
GCCCTGAAGG ACGTCAAGGG CCAGATCGGG GCCCCGATGC CCGGCAAGGT GATCGACATC 
AAGGTGAAGG TGGGGGACAA GGTGACCAAG GGGACGCCGC TGTGCGTGCT GAGCGCCATG 
AAGATGGAGA CCATGGTGAC GGCGCCCCTG GAGGGCACCA TCGCCAAGGT CCACGTGACT 
CGCGACATGA CTCTGGAGGG CGACGACCTC ATCTTGGAGA TCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_007502652.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007502714.1 Monodelphis domestica pyruvate carboxylase (PC), transcript variant X3, mRNA.

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CloneID	OMc31726
Clone ID Related Accession (Same CDS sequence)	XM_007502651.2 , XM_001364491.3 , XM_007502652.2
Accession Version	XM_007502652.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008808.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007502652.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TTCATTTGGC CCAAAGGGGC CTAAGGCTCC TAAGTATCCG ACATGCCTCC 
ATGATGCCCC CCTCAAACGC CCAACACATC AAGTACAAGC CCATCAAGAA AGTCATGGTG 
GCCAACCGAG GTGAGATAGC CATTCGAGTG TTCCGGGCCT GCACGGAGCT TGGCATCCGC 
ACCGTGGCCG TGTATTCCGA GCAGGACACT GGGCAGATGC ATCGGCAGAA GGCAGATGAG 
GCTTACCTCA TTGGCCGTGG TCTTAGCCCA GTGCAGGCCT ACCTGAACAT CGCAGACATC 
ATCAAGGTGG CCAAGGAGAA TGATGTCGAT GCCATTCACC CCGGCTATGG GTTCTTGTCG 
GAGCGTGCTG ATTTTGCTCA GGCCTGTCTG GATGCTGGGA TCAAATTCAT TGGCCCCAGC 
CCTGAAGTGG TGCGCAAGAT GGGGGACAAG GTGAGGGCCC GGCCCATTCC CATTGCTGCA 
GGTGTCCCAG TGGTTCCAGG CACAGACACC CCCATCTCCT CCTTACACGA GGCTCACGAA 
TTCTCCAAAA CCTATGGCCT CCCAATTATC TTCAAAGCTG CCTATGGAGG CGGGGGTCGT 
GGCATGAGGG TCGTACAAAA CTTTGAGGAA TTGGATGAGA ATTTTAACCG GGCCTATTCC 
GAGGCCCTGG CCGCCTTTGG GGATGGGACT CTGTTTGTGG AAAAATTCAT TGAGAATCCA 
CGCCACATTG AAGTGCAAAT TCTGGGGGAC CAGCATGGGA ACATTGTGCA CCTCTACGAA 
CGGGACTGCT CGATCCAGCG GAGGCATCAG AAGGTGGTGG AGATTGCTCC GGCCACTCTC 
CTGGACACCC AGCTCCGGAA TCGCCTGACC AGTGATTCGG TCAAGTTAGG CAAGCAGGTG 
GGCTATGAGA ACGCTGGCAC AGTGGAGTTC CTCATAGACA AACACGGCAA ACACTATTTC 
ATCGAGGTCA ATTCTCGCCT ACAAGTGGAG CACACGGTCA CCGAGGAGAT CACCCACGTA 
GACCTGGTCC ATGCCCAGAT CCATGTAGCT GAGGGCCGGA GCCTGCCGGA TCTGGGCCTT 
CTGCAAGAGA ATATTCGAAT CAGCGGTTGT GCAATCCAGT GTCGGGTGAC AACAGAGGAC 
CCGTCTCGAG GCTTCCAGCC AGACACTGGC CGGATCGAGG TGTTCCGTAG TGGTGAGGGG 
ATGGGCATCC GCTTGGACAA CGCCTCTGCC TTCCAAGGGT CAGTCATCTC CCCGCACTAC 
GATTCCCTGT TGGTCAAAGT CATAGCCCAT GGAAAGGACC ACCCCACGGC GGCCACCAAG 
ATGAGCCGGG CACTAGCCGA GTTCCGGGTC CGAGGGGTCA AGACCAACAT CCCCTTCCTG 
CAGAATGTTC TCAACAACCA GCAGTTCCTG GCGGGCACCG TGGATACCCA GTTCATCGAT 
TCAAACCCTG AGCTCTTCCA GATGCGGCCT GTTCAGAACC GGGCCCAGAA GCTGCTGCAC 
TACCTGGGTC ACGTCATGGT GAATGGGCCC CAGACACCGA TCCCGGTCAA GGCAAGCTCC 
AGCCCCACGG ATCCCATCAT CCCCCCCGTC CCCATGGGCC CACCTCCTCC TGGTTTCAGA 
GACATCCTGC TGCGTGAGGG TCCCGAGGGC TTTGCCCGGG CCGTGCGGAA CCACAAGGGG 
CTGCTGCTAA TGGACACGAC CTTCCGGGAT GCCCATCAGT CCCTGCTGGC CACACGAGTG 
AGGACCTACG ACCTCAAGAA GATCGCCCCT TATGTCGCCC ACAGTTTCAA CAGCCTTTTC 
AGTCTGGAGA ACTGGGGGGG AGCCACGTTT GATGTTGCCA TGCGCTTCCT GTACGAGTGT 
CCGTGGCGGA GGCTACAGGA GCTCAGAGAG CTCATCCCCA ACATCCCCTT CCAGATGCTG 
CTCCGGGGTG CCAACGCTGT GGGCTACACC AACTACCCGG ACAACGTGGT CTTTAAGTTC 
TGTGAGGTGG CCAAGGAGAA CGGGATGGAC GTGTTCCGTG TCTTTGATTC CCTCAACTAC 
ATGCCCAATC TGCTGCTGGG CATGGAGGCG GCAGGCAGTG CCGGAGGTGT GGTGGAGGCA 
GCCATCTCCT ACACGGGCGA CGTGGCCGAC CCCACGCGCA CCAAGTATTC CCTGAAGTAC 
TACATGGACC TGGCTGAGGA GCTGGTGCGG GCTGGGACAC ACATCCTGAG CATCAAGGAC 
ATGGCAGGCC TTCTGAAGCC GGCTGCATGC ACCATGCTGG TGGGTGCCCT CCGAGACCGC 
TTCCCAGAGC TCCCCCTGCA CATCCACACC CATGACACGG CCGGCTCAGG TGTGGCCAAT 
ATGCTGGCCT GTGCCCAGGC AGGGGCAGAT GTGGTGGATG TGGCTGTGGA CTCCATGTCG 
GGGATCACGT CCCAGCCCAG CATGGGGGCC CTGGTGGCCT GCACTCTGGG GACCCCCTTC 
GACACAGGGC TACCTTTGGA GCGGGTGTTT GACTACAGCG AGTACTGGGA GGGGGCCCGG 
GGGCTATACG CCCCCTTCGA CTGCACGGCC ACCATGAAAT CCGGGAACTC GGATGTGTAC 
GAGAACGAGA TCCCGGGAGG CCAGTACACA AACCTGCACT TCCAGCCTTA CAGCATGGGG 
CTTGGCACAA AGTTCAAAGA AGTCAAAAAG GCCTATGTGA GGGGCAACCA GATGCTGGGG 
GACCTCATCA AGGTGACCCC TTCCTCCAAG ATCGTGGGGG ACCTGGCCCA GTTCATGGTG 
CAGAATGGGC TGAGCCGAGC CGACGTGGAG GCCAAGGCCG AGGAACTGTC CTTCCCGCGC 
TCGGTGGTGG AGTTCTTGCA GGGCTACATT GGCATACCCT ATGGGGGCTT CCCCGAACCC 
TTTCGATCCA AGGTCCTAAA GGACCTGCCC CGGGTCGAGG GGCGGCCGGG CGCTTCCATG 
ACCGCCCTGG ACTTGAGGAA GCTGGAGGAT GAGCTGAAGG AACGCAACGA CGAGATAACC 
CCCGAGGACG TGCTCTCGGC CGCCATGTAT CCCGACGTCT TCCAGCAGTT CAAGGACTTC 
ACGGCCACCT TCGGGCCCCT GGAGTGCCTC AACACCCGCC TCTTCCTGCA AGGGCCCAAG 
GTCGCCGAAG AGTTTGAGGT AGAGCTGGAA CGGGGCAAAA CCCTGCACAT CAAGGCCTTG 
GCCCTGGGGG ACGTGAACCG GGCCGGGCAG AGGCAGGTGT TCTTCGAGCT CAACGGGCAG 
GTGCGCTCCA TCCTGGTCAA GGACACCCAG GCCATGAAGG AGATGCACTT CCACCCCAAG 
GCCCTGAAGG ACGTCAAGGG CCAGATCGGG GCCCCGATGC CCGGCAAGGT GATCGACATC 
AAGGTGAAGG TGGGGGACAA GGTGACCAAG GGGACGCCGC TGTGCGTGCT GAGCGCCATG 
AAGATGGAGA CCATGGTGAC GGCGCCCCTG GAGGGCACCA TCGCCAAGGT CCACGTGACT 
CGCGACATGA CTCTGGAGGG CGACGACCTC ATCTTGGAGA TCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007502714.1
CDS	165..3692
Translation	MLKFHLAQRGLRLLSIRHASMMPPSNAQHIKYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLSPVQAYLNIADIIKVAKENDVDAIHPGYGFLSERADFAQACLDAGIKFIGPSPEVVRKMGDKVRARPIPIAAGVPVVPGTDTPISSLHEAHEFSKTYGLPIIFKAAYGGGGRGMRVVQNFEELDENFNRAYSEALAAFGDGTLFVEKFIENPRHIEVQILGDQHGNIVHLYERDCSIQRRHQKVVEIAPATLLDTQLRNRLTSDSVKLGKQVGYENAGTVEFLIDKHGKHYFIEVNSRLQVEHTVTEEITHVDLVHAQIHVAEGRSLPDLGLLQENIRISGCAIQCRVTTEDPSRGFQPDTGRIEVFRSGEGMGIRLDNASAFQGSVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDSNPELFQMRPVQNRAQKLLHYLGHVMVNGPQTPIPVKASSSPTDPIIPPVPMGPPPPGFRDILLREGPEGFARAVRNHKGLLLMDTTFRDAHQSLLATRVRTYDLKKIAPYVAHSFNSLFSLENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYMPNLLLGMEAAGSAGGVVEAAISYTGDVADPTRTKYSLKYYMDLAEELVRAGTHILSIKDMAGLLKPAACTMLVGALRDRFPELPLHIHTHDTAGSGVANMLACAQAGADVVDVAVDSMSGITSQPSMGALVACTLGTPFDTGLPLERVFDYSEYWEGARGLYAPFDCTATMKSGNSDVYENEIPGGQYTNLHFQPYSMGLGTKFKEVKKAYVRGNQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRADVEAKAEELSFPRSVVEFLQGYIGIPYGGFPEPFRSKVLKDLPRVEGRPGASMTALDLRKLEDELKERNDEITPEDVLSAAMYPDVFQQFKDFTATFGPLECLNTRLFLQGPKVAEEFEVELERGKTLHIKALALGDVNRAGQRQVFFELNGQVRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVKVGDKVTKGTPLCVLSAMKMETMVTAPLEGTIAKVHVTRDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_007502652.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica pyruvate carboxylase (PC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007502652.2

ORF Insert Sequence:

ATGTTGAAGT TTCATTTGGC CCAAAGGGGC CTAAGGCTCC TAAGTATCCG ACATGCCTCC 
ATGATGCCCC CCTCAAACGC CCAACACATC AAGTACAAGC CCATCAAGAA AGTCATGGTG 
GCCAACCGAG GTGAGATAGC CATTCGAGTG TTCCGGGCCT GCACGGAGCT TGGCATCCGC 
ACCGTGGCCG TGTATTCCGA GCAGGACACT GGGCAGATGC ATCGGCAGAA GGCAGATGAG 
GCTTACCTCA TTGGCCGTGG TCTTAGCCCA GTGCAGGCCT ACCTGAACAT CGCAGACATC 
ATCAAGGTGG CCAAGGAGAA TGATGTCGAT GCCATTCACC CCGGCTATGG GTTCTTGTCG 
GAGCGTGCTG ATTTTGCTCA GGCCTGTCTG GATGCTGGGA TCAAATTCAT TGGCCCCAGC 
CCTGAAGTGG TGCGCAAGAT GGGGGACAAG GTGAGGGCCC GGCCCATTCC CATTGCTGCA 
GGTGTCCCAG TGGTTCCAGG CACAGACACC CCCATCTCCT CCTTACACGA GGCTCACGAA 
TTCTCCAAAA CCTATGGCCT CCCAATTATC TTCAAAGCTG CCTATGGAGG CGGGGGTCGT 
GGCATGAGGG TCGTACAAAA CTTTGAGGAA TTGGATGAGA ATTTTAACCG GGCCTATTCC 
GAGGCCCTGG CCGCCTTTGG GGATGGGACT CTGTTTGTGG AAAAATTCAT TGAGAATCCA 
CGCCACATTG AAGTGCAAAT TCTGGGGGAC CAGCATGGGA ACATTGTGCA CCTCTACGAA 
CGGGACTGCT CGATCCAGCG GAGGCATCAG AAGGTGGTGG AGATTGCTCC GGCCACTCTC 
CTGGACACCC AGCTCCGGAA TCGCCTGACC AGTGATTCGG TCAAGTTAGG CAAGCAGGTG 
GGCTATGAGA ACGCTGGCAC AGTGGAGTTC CTCATAGACA AACACGGCAA ACACTATTTC 
ATCGAGGTCA ATTCTCGCCT ACAAGTGGAG CACACGGTCA CCGAGGAGAT CACCCACGTA 
GACCTGGTCC ATGCCCAGAT CCATGTAGCT GAGGGCCGGA GCCTGCCGGA TCTGGGCCTT 
CTGCAAGAGA ATATTCGAAT CAGCGGTTGT GCAATCCAGT GTCGGGTGAC AACAGAGGAC 
CCGTCTCGAG GCTTCCAGCC AGACACTGGC CGGATCGAGG TGTTCCGTAG TGGTGAGGGG 
ATGGGCATCC GCTTGGACAA CGCCTCTGCC TTCCAAGGGT CAGTCATCTC CCCGCACTAC 
GATTCCCTGT TGGTCAAAGT CATAGCCCAT GGAAAGGACC ACCCCACGGC GGCCACCAAG 
ATGAGCCGGG CACTAGCCGA GTTCCGGGTC CGAGGGGTCA AGACCAACAT CCCCTTCCTG 
CAGAATGTTC TCAACAACCA GCAGTTCCTG GCGGGCACCG TGGATACCCA GTTCATCGAT 
TCAAACCCTG AGCTCTTCCA GATGCGGCCT GTTCAGAACC GGGCCCAGAA GCTGCTGCAC 
TACCTGGGTC ACGTCATGGT GAATGGGCCC CAGACACCGA TCCCGGTCAA GGCAAGCTCC 
AGCCCCACGG ATCCCATCAT CCCCCCCGTC CCCATGGGCC CACCTCCTCC TGGTTTCAGA 
GACATCCTGC TGCGTGAGGG TCCCGAGGGC TTTGCCCGGG CCGTGCGGAA CCACAAGGGG 
CTGCTGCTAA TGGACACGAC CTTCCGGGAT GCCCATCAGT CCCTGCTGGC CACACGAGTG 
AGGACCTACG ACCTCAAGAA GATCGCCCCT TATGTCGCCC ACAGTTTCAA CAGCCTTTTC 
AGTCTGGAGA ACTGGGGGGG AGCCACGTTT GATGTTGCCA TGCGCTTCCT GTACGAGTGT 
CCGTGGCGGA GGCTACAGGA GCTCAGAGAG CTCATCCCCA ACATCCCCTT CCAGATGCTG 
CTCCGGGGTG CCAACGCTGT GGGCTACACC AACTACCCGG ACAACGTGGT CTTTAAGTTC 
TGTGAGGTGG CCAAGGAGAA CGGGATGGAC GTGTTCCGTG TCTTTGATTC CCTCAACTAC 
ATGCCCAATC TGCTGCTGGG CATGGAGGCG GCAGGCAGTG CCGGAGGTGT GGTGGAGGCA 
GCCATCTCCT ACACGGGCGA CGTGGCCGAC CCCACGCGCA CCAAGTATTC CCTGAAGTAC 
TACATGGACC TGGCTGAGGA GCTGGTGCGG GCTGGGACAC ACATCCTGAG CATCAAGGAC 
ATGGCAGGCC TTCTGAAGCC GGCTGCATGC ACCATGCTGG TGGGTGCCCT CCGAGACCGC 
TTCCCAGAGC TCCCCCTGCA CATCCACACC CATGACACGG CCGGCTCAGG TGTGGCCAAT 
ATGCTGGCCT GTGCCCAGGC AGGGGCAGAT GTGGTGGATG TGGCTGTGGA CTCCATGTCG 
GGGATCACGT CCCAGCCCAG CATGGGGGCC CTGGTGGCCT GCACTCTGGG GACCCCCTTC 
GACACAGGGC TACCTTTGGA GCGGGTGTTT GACTACAGCG AGTACTGGGA GGGGGCCCGG 
GGGCTATACG CCCCCTTCGA CTGCACGGCC ACCATGAAAT CCGGGAACTC GGATGTGTAC 
GAGAACGAGA TCCCGGGAGG CCAGTACACA AACCTGCACT TCCAGCCTTA CAGCATGGGG 
CTTGGCACAA AGTTCAAAGA AGTCAAAAAG GCCTATGTGA GGGGCAACCA GATGCTGGGG 
GACCTCATCA AGGTGACCCC TTCCTCCAAG ATCGTGGGGG ACCTGGCCCA GTTCATGGTG 
CAGAATGGGC TGAGCCGAGC CGACGTGGAG GCCAAGGCCG AGGAACTGTC CTTCCCGCGC 
TCGGTGGTGG AGTTCTTGCA GGGCTACATT GGCATACCCT ATGGGGGCTT CCCCGAACCC 
TTTCGATCCA AGGTCCTAAA GGACCTGCCC CGGGTCGAGG GGCGGCCGGG CGCTTCCATG 
ACCGCCCTGG ACTTGAGGAA GCTGGAGGAT GAGCTGAAGG AACGCAACGA CGAGATAACC 
CCCGAGGACG TGCTCTCGGC CGCCATGTAT CCCGACGTCT TCCAGCAGTT CAAGGACTTC 
ACGGCCACCT TCGGGCCCCT GGAGTGCCTC AACACCCGCC TCTTCCTGCA AGGGCCCAAG 
GTCGCCGAAG AGTTTGAGGT AGAGCTGGAA CGGGGCAAAA CCCTGCACAT CAAGGCCTTG 
GCCCTGGGGG ACGTGAACCG GGCCGGGCAG AGGCAGGTGT TCTTCGAGCT CAACGGGCAG 
GTGCGCTCCA TCCTGGTCAA GGACACCCAG GCCATGAAGG AGATGCACTT CCACCCCAAG 
GCCCTGAAGG ACGTCAAGGG CCAGATCGGG GCCCCGATGC CCGGCAAGGT GATCGACATC 
AAGGTGAAGG TGGGGGACAA GGTGACCAAG GGGACGCCGC TGTGCGTGCT GAGCGCCATG 
AAGATGGAGA CCATGGTGAC GGCGCCCCTG GAGGGCACCA TCGCCAAGGT CCACGTGACT 
CGCGACATGA CTCTGGAGGG CGACGACCTC ATCTTGGAGA TCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PC cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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