PLCB4 cDNA ORF clone, Lipotes vexillifer(Yangtze River dolphin)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLCB4
Entrez Gene ID	103078639
Full Name	phospholipase C beta 4
Gene Type	protein-coding
Organism	Lipotes vexillifer(Yangtze River dolphin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007452036.1	XP_007452098.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
XM_007452037.1	XP_007452099.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
XM_007452038.1	XP_007452100.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X3

Pathways from BioSystems
Gene Ontology

The following PLCB4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCB4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLi42385	XM_007452036.1 Latest version!	Lipotes vexillifer phospholipase C, beta 4 (PLCB4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OLi42386	XM_007452037.1 Latest version!	Lipotes vexillifer phospholipase C, beta 4 (PLCB4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OLi42387	XM_007452038.1 Latest version!	Lipotes vexillifer phospholipase C, beta 4 (PLCB4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLCB4 ( XM_007452036.1 ) cDNA ORF clone, Lipotes vexillifer(Yangtze River dolphin) -> XP_007452098.1 Lipotes vexillifer phospholipase C, beta 4 (PLCB4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLi42385
Clone ID Related Accession (Same CDS sequence)	XM_007452036.1
Accession Version	XM_007452036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1396195200000
Organism	Lipotes vexillifer(Yangtze River dolphin)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006778935.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lipotes vexillifer Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCGTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAG 
GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG ACAAGTTCTA 
GAATGTTCTC TCATCAACAG TGTTCGACTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTATTGG AAAGTCAGAA AATGACCTGG AAGGGCGGGT AGTTTGTGTC 
TGCAGTGGTA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATTCAGAA 
GTAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATAC ACAACTTCAG GGCCAACAAT 
GTCAGTCCCA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACC AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCACTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAA 
CCTGCAGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTC 
CCATTTTATG ATACTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAC 
TTGAAGAAAA AAGGCCAAAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCAG TCTTCCTAGA TCGCCTAGAG CTTTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGCAAG 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGC 
ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCT 
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GCGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGGCC TTTGCCATCC CCAAATGACC TCAAAAGAAA AATACTCATC 
AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TTGAAGCTTT GAAAAGTATG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCTTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAAGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCGGACG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTTAAGAA GGGCCTGGTA 
ACTATAGAAG ATGAGCAGGC ATGGATGGCA TCTTATAAAT ATGTAGGCGC CACCACTAAT 
ATCCATCCAT ATTTGTCCAC AATGATCAAC TATGCACAGC CCGTAAAGTT TCAAGGTTTC 
CACGTGGCTG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA GTCAGTTGGT 
CTAGGCTACT TGAAGACACA TGCGATTGAA TTTGTGAATT ATAACAAACG GCAAATGAGC 
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG 
AACGCTGGCT GCCAGATGGT TTCGCTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG 
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG 
AGACGGCCCG ATAGAACATT TGATCCCTTC TCTGAAACGC CTGTTGATGG GGTTATTGCA 
GCCACTTGCT CAGTGCAGGT CATATCAGGT CAGTTCTTAT CAGATAAAAA AATTGGCACA 
TATGTAGAAG TGGATATGTA TGGGTTGCCC ACCGACACTA TCCGGAAGGA ATTCCGAACA 
CGCATGGTGA TGAACAATGG ACTCAATCCA GTTTACAATG AAGAATCATT TGTATTTCGG 
AAGGTGATCC TCCCTGACCT GGCAGTCTTG AGACTTGCAG TGTATGATGA CAACAACAAG 
CTGATTGGCC AGAGGATTCT GCCACTGGAT GGCCTCCAAG CAGGCTATCG ACACATTTCT 
CTTCGAAATG AGGGAAATAA ACCTTTATCA CTGCCAACAA TTTTCTGCAA TATTGTTCTT 
AAAACATATG TGCCTGATGG ATTTGGAGAT ATTGTGGATG CTTTATCAGA TCCAAAGAAA 
TTTCTTTCCA TTACGGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT 
GACATCGCCG ACGTGCCCAG TGACACGTCA AAGAATGACA AGAAAGGGAA AGCCAACACA 
GCGAAAGCAA GTGTGACCCC TCAGAGCAGC TCAGAGCTCA GGCCCACCAC CACGGCTGCC 
CTGGGAGCTG GCCTGGAGGC CAAGAAAGGT ATTGAACTGA TCCCTCAAGT GAGGATAGAA 
GATTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAACAGAA GGAGCTCAAT 
TCTTTAAAGA AGAAACATGC AAAGGAGCAC AGTACCATGC AGAAGCTACA TTGCACACAA 
GTTGACAAAA TTGTGGCACA GTATGACAAG GAGAAGTCAA CTCATGAGAA AATCCTAGAG 
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTTGAAA TGAAGAAAGA AACAGAAATT 
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AGGAGATCGT GGCGCAGCAC 
ACAAAAGAGT GGTCAGACAT GATCAACACC CACAGTGCAG AGGAGCAGGA GATCCGGGAT 
CTGCACCTCA GCCAGCAGTG CGAGCTGCTC AAAAAGCTCC TGATCAACGC CCACGAGCAG 
CAGACCCAGC AGTTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG 
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA 
GCAGAACGGG AAAGGCGGGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA 
GAAAGAAAGC GACTCGCAAT GAAGCAGTCC AAAGAAATGG ACCAGTTGAA AAAAGTCCAG 
CTTGAACACC TCGAATTCCT AGAGAAACAG AACGAGCAGC TTTTGAAATC CTGTCATGCA 
GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA 
CCGCAGACCA GCAACAGTTG TGTGAAACTC CAGGACACAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007452098.1
CDS	10..3594
Translation	MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEAIGKSENDLEGRVVCVCSGTDLVNISFTYMVAENSEVTKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDTKRAMQIIEMYEPDEDLKKKGQISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSMMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKGLVTIEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRLAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKASVTPQSSSELRPTTTAALGAGLEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKSTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEIRDLHLSQQCELLKKLLINAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSCVKLQDTN

Target ORF information:

RefSeq Version	XM_007452036.1
Organism	Lipotes vexillifer(Yangtze River dolphin)
Definition	Lipotes vexillifer phospholipase C, beta 4 (PLCB4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007452036.1

ORF Insert Sequence:

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCGTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAG 
GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG ACAAGTTCTA 
GAATGTTCTC TCATCAACAG TGTTCGACTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTATTGG AAAGTCAGAA AATGACCTGG AAGGGCGGGT AGTTTGTGTC 
TGCAGTGGTA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATTCAGAA 
GTAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATAC ACAACTTCAG GGCCAACAAT 
GTCAGTCCCA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACC AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCACTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAA 
CCTGCAGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTC 
CCATTTTATG ATACTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAC 
TTGAAGAAAA AAGGCCAAAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCAG TCTTCCTAGA TCGCCTAGAG CTTTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGCAAG 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGC 
ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCT 
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GCGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGGCC TTTGCCATCC CCAAATGACC TCAAAAGAAA AATACTCATC 
AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TTGAAGCTTT GAAAAGTATG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCTTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAAGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCGGACG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTTAAGAA GGGCCTGGTA 
ACTATAGAAG ATGAGCAGGC ATGGATGGCA TCTTATAAAT ATGTAGGCGC CACCACTAAT 
ATCCATCCAT ATTTGTCCAC AATGATCAAC TATGCACAGC CCGTAAAGTT TCAAGGTTTC 
CACGTGGCTG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA GTCAGTTGGT 
CTAGGCTACT TGAAGACACA TGCGATTGAA TTTGTGAATT ATAACAAACG GCAAATGAGC 
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG 
AACGCTGGCT GCCAGATGGT TTCGCTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG 
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG 
AGACGGCCCG ATAGAACATT TGATCCCTTC TCTGAAACGC CTGTTGATGG GGTTATTGCA 
GCCACTTGCT CAGTGCAGGT CATATCAGGT CAGTTCTTAT CAGATAAAAA AATTGGCACA 
TATGTAGAAG TGGATATGTA TGGGTTGCCC ACCGACACTA TCCGGAAGGA ATTCCGAACA 
CGCATGGTGA TGAACAATGG ACTCAATCCA GTTTACAATG AAGAATCATT TGTATTTCGG 
AAGGTGATCC TCCCTGACCT GGCAGTCTTG AGACTTGCAG TGTATGATGA CAACAACAAG 
CTGATTGGCC AGAGGATTCT GCCACTGGAT GGCCTCCAAG CAGGCTATCG ACACATTTCT 
CTTCGAAATG AGGGAAATAA ACCTTTATCA CTGCCAACAA TTTTCTGCAA TATTGTTCTT 
AAAACATATG TGCCTGATGG ATTTGGAGAT ATTGTGGATG CTTTATCAGA TCCAAAGAAA 
TTTCTTTCCA TTACGGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT 
GACATCGCCG ACGTGCCCAG TGACACGTCA AAGAATGACA AGAAAGGGAA AGCCAACACA 
GCGAAAGCAA GTGTGACCCC TCAGAGCAGC TCAGAGCTCA GGCCCACCAC CACGGCTGCC 
CTGGGAGCTG GCCTGGAGGC CAAGAAAGGT ATTGAACTGA TCCCTCAAGT GAGGATAGAA 
GATTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAACAGAA GGAGCTCAAT 
TCTTTAAAGA AGAAACATGC AAAGGAGCAC AGTACCATGC AGAAGCTACA TTGCACACAA 
GTTGACAAAA TTGTGGCACA GTATGACAAG GAGAAGTCAA CTCATGAGAA AATCCTAGAG 
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTTGAAA TGAAGAAAGA AACAGAAATT 
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AGGAGATCGT GGCGCAGCAC 
ACAAAAGAGT GGTCAGACAT GATCAACACC CACAGTGCAG AGGAGCAGGA GATCCGGGAT 
CTGCACCTCA GCCAGCAGTG CGAGCTGCTC AAAAAGCTCC TGATCAACGC CCACGAGCAG 
CAGACCCAGC AGTTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG 
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA 
GCAGAACGGG AAAGGCGGGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA 
GAAAGAAAGC GACTCGCAAT GAAGCAGTCC AAAGAAATGG ACCAGTTGAA AAAAGTCCAG 
CTTGAACACC TCGAATTCCT AGAGAAACAG AACGAGCAGC TTTTGAAATC CTGTCATGCA 
GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA 
CCGCAGACCA GCAACAGTTG TGTGAAACTC CAGGACACAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_007452037.1 ) cDNA ORF clone, Lipotes vexillifer(Yangtze River dolphin) -> XP_007452099.1 Lipotes vexillifer phospholipase C, beta 4 (PLCB4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLi42386
Clone ID Related Accession (Same CDS sequence)	XM_007452037.1
Accession Version	XM_007452037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1396195200000
Organism	Lipotes vexillifer(Yangtze River dolphin)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006778935.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lipotes vexillifer Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCGTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAG 
GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG ACAAGTTCTA 
GAATGTTCTC TCATCAACAG TGTTCGACTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTATTGG AAAGTCAGAA AATGACCTGG AAGGGCGGGT AGTTTGTGTC 
TGCAGTGGTA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATTCAGAA 
GTAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATAC ACAACTTCAG GGCCAACAAT 
GTCAGTCCCA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACC AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCACTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAA 
CCTGCAGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTC 
CCATTTTATG ATACTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAC 
TTGAAGAAAA AAGGCCAAAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCAG TCTTCCTAGA TCGCCTAGAG CTTTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGCAAG 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGC 
ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCT 
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GCGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGGCC TTTGCCATCC CCAAATGACC TCAAAAGAAA AATACTCATC 
AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TTGAAGCTTT GAAAAGTATG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCTTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAAGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCGGACG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTTAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTAACTA TAGAAGATGA GCAGGCATGG 
ATGGCATCTT ATAAATATGT AGGCGCCACC ACTAATATCC ATCCATATTT GTCCACAATG 
ATCAACTATG CACAGCCCGT AAAGTTTCAA GGTTTCCACG TGGCTGAAGA ACGCAATATT 
CATTATAACA TGTCTTCTTT TAATGAGTCA GTTGGTCTAG GCTACTTGAA GACACATGCG 
ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGCCGCA TTTACCCCAA GGGAGGCCGA 
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT 
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGAC GGCCCGATAG AACATTTGAT 
CCCTTCTCTG AAACGCCTGT TGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAAAAAATT GGCACATATG TAGAAGTGGA TATGTATGGG 
TTGCCCACCG ACACTATCCG GAAGGAATTC CGAACACGCA TGGTGATGAA CAATGGACTC 
AATCCAGTTT ACAATGAAGA ATCATTTGTA TTTCGGAAGG TGATCCTCCC TGACCTGGCA 
GTCTTGAGAC TTGCAGTGTA TGATGACAAC AACAAGCTGA TTGGCCAGAG GATTCTGCCA 
CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCTCTTC GAAATGAGGG AAATAAACCT 
TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGACGT GCCCAGTGAC 
ACGTCAAAGA ATGACAAGAA AGGGAAAGCC AACACAGCGA AAGCAAGTGT GACCCCTCAG 
AGCAGCTCAG AGCTCAGGCC CACCACCACG GCTGCCCTGG GAGCTGGCCT GGAGGCCAAG 
AAAGGTATTG AACTGATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC 
TTGAAGCATT TAAAGAAACA ACAGAAGGAG CTCAATTCTT TAAAGAAGAA ACATGCAAAG 
GAGCACAGTA CCATGCAGAA GCTACATTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT 
GACAAGGAGA AGTCAACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA 
AGTAATTGTC TTGAAATGAA GAAAGAAACA GAAATTAAAA TTCAGACGCT GACATCAGAT 
CACAAATCTA AGGTCAAGGA GATCGTGGCG CAGCACACAA AAGAGTGGTC AGACATGATC 
AACACCCACA GTGCAGAGGA GCAGGAGATC CGGGATCTGC ACCTCAGCCA GCAGTGCGAG 
CTGCTCAAAA AGCTCCTGAT CAACGCCCAC GAGCAGCAGA CCCAGCAGTT GAAACTGTCC 
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC 
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGGGTCAGG 
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGCGACT CGCAATGAAG 
CAGTCCAAAG AAATGGACCA GTTGAAAAAA GTCCAGCTTG AACACCTCGA ATTCCTAGAG 
AAACAGAACG AGCAGGCGAA GGAGATGCAG CAGATGGTGA AATTGGAAGC CGAGATGGAC 
CGCAGACCAG CAACAGTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007452099.1
CDS	1..3564
Translation	MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEAIGKSENDLEGRVVCVCSGTDLVNISFTYMVAENSEVTKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDTKRAMQIIEMYEPDEDLKKKGQISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSMMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKASDDLEHENNKKGLVTIEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRLAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKASVTPQSSSELRPTTTAALGAGLEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKSTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEIRDLHLSQQCELLKKLLINAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQAKEMQQMVKLEAEMDRRPATVV

Target ORF information:

RefSeq Version	XM_007452037.1
Organism	Lipotes vexillifer(Yangtze River dolphin)
Definition	Lipotes vexillifer phospholipase C, beta 4 (PLCB4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007452037.1

ORF Insert Sequence:

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCGTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAG 
GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG ACAAGTTCTA 
GAATGTTCTC TCATCAACAG TGTTCGACTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTATTGG AAAGTCAGAA AATGACCTGG AAGGGCGGGT AGTTTGTGTC 
TGCAGTGGTA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATTCAGAA 
GTAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATAC ACAACTTCAG GGCCAACAAT 
GTCAGTCCCA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACC AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCACTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAA 
CCTGCAGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTC 
CCATTTTATG ATACTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAC 
TTGAAGAAAA AAGGCCAAAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCAG TCTTCCTAGA TCGCCTAGAG CTTTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGCAAG 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGC 
ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCT 
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GCGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGGCC TTTGCCATCC CCAAATGACC TCAAAAGAAA AATACTCATC 
AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TTGAAGCTTT GAAAAGTATG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCTTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAAGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCGGACG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTTAAGAA GGCTTCAGAT 
GACCTTGAAC ATGAAAACAA CAAAAAGGGC CTGGTAACTA TAGAAGATGA GCAGGCATGG 
ATGGCATCTT ATAAATATGT AGGCGCCACC ACTAATATCC ATCCATATTT GTCCACAATG 
ATCAACTATG CACAGCCCGT AAAGTTTCAA GGTTTCCACG TGGCTGAAGA ACGCAATATT 
CATTATAACA TGTCTTCTTT TAATGAGTCA GTTGGTCTAG GCTACTTGAA GACACATGCG 
ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGCCGCA TTTACCCCAA GGGAGGCCGA 
GTCGATTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG 
CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT 
GGATCGTGCG GGTACCTTCT CAAACCAGAT TTCATGAGAC GGCCCGATAG AACATTTGAT 
CCCTTCTCTG AAACGCCTGT TGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA 
TCAGGTCAGT TCTTATCAGA TAAAAAAATT GGCACATATG TAGAAGTGGA TATGTATGGG 
TTGCCCACCG ACACTATCCG GAAGGAATTC CGAACACGCA TGGTGATGAA CAATGGACTC 
AATCCAGTTT ACAATGAAGA ATCATTTGTA TTTCGGAAGG TGATCCTCCC TGACCTGGCA 
GTCTTGAGAC TTGCAGTGTA TGATGACAAC AACAAGCTGA TTGGCCAGAG GATTCTGCCA 
CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCTCTTC GAAATGAGGG AAATAAACCT 
TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT 
GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA 
GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGACGT GCCCAGTGAC 
ACGTCAAAGA ATGACAAGAA AGGGAAAGCC AACACAGCGA AAGCAAGTGT GACCCCTCAG 
AGCAGCTCAG AGCTCAGGCC CACCACCACG GCTGCCCTGG GAGCTGGCCT GGAGGCCAAG 
AAAGGTATTG AACTGATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC 
TTGAAGCATT TAAAGAAACA ACAGAAGGAG CTCAATTCTT TAAAGAAGAA ACATGCAAAG 
GAGCACAGTA CCATGCAGAA GCTACATTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT 
GACAAGGAGA AGTCAACTCA TGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAGGGGGGA 
AGTAATTGTC TTGAAATGAA GAAAGAAACA GAAATTAAAA TTCAGACGCT GACATCAGAT 
CACAAATCTA AGGTCAAGGA GATCGTGGCG CAGCACACAA AAGAGTGGTC AGACATGATC 
AACACCCACA GTGCAGAGGA GCAGGAGATC CGGGATCTGC ACCTCAGCCA GCAGTGCGAG 
CTGCTCAAAA AGCTCCTGAT CAACGCCCAC GAGCAGCAGA CCCAGCAGTT GAAACTGTCC 
CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAATAGC 
AAAGCCATCA GCCAAGATAA ATCTATCAAG AATAAAGCAG AACGGGAAAG GCGGGTCAGG 
GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGCGACT CGCAATGAAG 
CAGTCCAAAG AAATGGACCA GTTGAAAAAA GTCCAGCTTG AACACCTCGA ATTCCTAGAG 
AAACAGAACG AGCAGGCGAA GGAGATGCAG CAGATGGTGA AATTGGAAGC CGAGATGGAC 
CGCAGACCAG CAACAGTTGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB4 ( XM_007452038.1 ) cDNA ORF clone, Lipotes vexillifer(Yangtze River dolphin) -> XP_007452100.1 Lipotes vexillifer phospholipase C, beta 4 (PLCB4), transcript variant X3, mRNA.

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CloneID	OLi42387
Clone ID Related Accession (Same CDS sequence)	XM_007452038.1
Accession Version	XM_007452038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1396195200000
Organism	Lipotes vexillifer(Yangtze River dolphin)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006778935.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lipotes vexillifer Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCGTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAG 
GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG ACAAGTTCTA 
GAATGTTCTC TCATCAACAG TGTTCGACTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTATTGG AAAGTCAGAA AATGACCTGG AAGGGCGGGT AGTTTGTGTC 
TGCAGTGGTA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATTCAGAA 
GTAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATAC ACAACTTCAG GGCCAACAAT 
GTCAGTCCCA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACC AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCACTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAA 
CCTGCAGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTC 
CCATTTTATG ATACTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAC 
TTGAAGAAAA AAGGCCAAAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCAG TCTTCCTAGA TCGCCTAGAG CTTTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGCAAG 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGC 
ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCT 
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GCGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGGCC TTTGCCATCC CCAAATGACC TCAAAAGAAA AATACTCATC 
AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TTGAAGCTTT GAAAAGTATG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCTTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAAGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCGGACG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTTAAGAA GGGCCTGGTA 
ACTATAGAAG ATGAGCAGGC ATGGATGGCA TCTTATAAAT ATGTAGGCGC CACCACTAAT 
ATCCATCCAT ATTTGTCCAC AATGATCAAC TATGCACAGC CCGTAAAGTT TCAAGGTTTC 
CACGTGGCTG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA GTCAGTTGGT 
CTAGGCTACT TGAAGACACA TGCGATTGAA TTTGTGAATT ATAACAAACG GCAAATGAGC 
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG 
AACGCTGGCT GCCAGATGGT TTCGCTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG 
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG 
AGACGGCCCG ATAGAACATT TGATCCCTTC TCTGAAACGC CTGTTGATGG GGTTATTGCA 
GCCACTTGCT CAGTGCAGGT CATATCAGGT CAGTTCTTAT CAGATAAAAA AATTGGCACA 
TATGTAGAAG TGGATATGTA TGGGTTGCCC ACCGACACTA TCCGGAAGGA ATTCCGAACA 
CGCATGGTGA TGAACAATGG ACTCAATCCA GTTTACAATG AAGAATCATT TGTATTTCGG 
AAGGTGATCC TCCCTGACCT GGCAGTCTTG AGACTTGCAG TGTATGATGA CAACAACAAG 
CTGATTGGCC AGAGGATTCT GCCACTGGAT GGCCTCCAAG CAGGCTATCG ACACATTTCT 
CTTCGAAATG AGGGAAATAA ACCTTTATCA CTGCCAACAA TTTTCTGCAA TATTGTTCTT 
AAAACATATG TGCCTGATGG ATTTGGAGAT ATTGTGGATG CTTTATCAGA TCCAAAGAAA 
TTTCTTTCCA TTACGGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT 
GACATCGCCG ACGTGCCCAG TGACACGTCA AAGAATGACA AGAAAGGGAA AGCCAACACA 
GCGAAAGCAA GTGTGACCCC TCAGAGCAGC TCAGAGCTCA GGCCCACCAC CACGGCTGCC 
CTGGGAGCTG GCCTGGAGGC CAAGAAAGGT ATTGAACTGA TCCCTCAAGT GAGGATAGAA 
GATTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAACAGAA GGAGCTCAAT 
TCTTTAAAGA AGAAACATGC AAAGGAGCAC AGTACCATGC AGAAGCTACA TTGCACACAA 
GTTGACAAAA TTGTGGCACA GTATGACAAG GAGAAGTCAA CTCATGAGAA AATCCTAGAG 
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTTGAAA TGAAGAAAGA AACAGAAATT 
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AGGAGATCGT GGCGCAGCAC 
ACAAAAGAGT GGTCAGACAT GATCAACACC CACAGTGCAG AGGAGCAGGA GATCCGGGAT 
CTGCACCTCA GCCAGCAGTG CGAGCTGCTC AAAAAGCTCC TGATCAACGC CCACGAGCAG 
CAGACCCAGC AGTTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG 
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA 
GCAGAACGGG AAAGGCGGGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA 
GAAAGAAAGC GACTCGCAAT GAAGCAGTCC AAAGAAATGG ACCAGTTGAA AAAAGTCCAG 
CTTGAACACC TCGAATTCCT AGAGAAACAG AACGAGCAGG CGAAGGAGAT GCAGCAGATG 
GTGAAATTGG AAGCCGAGAT GGACCGCAGA CCAGCAACAG TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007452100.1
CDS	10..3537
Translation	MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSVRLGATPKDPKILAALEAIGKSENDLEGRVVCVCSGTDLVNISFTYMVAENSEVTKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDTKRAMQIIEMYEPDEDLKKKGQISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSMMEAGESAAPVNILEDDNEEEIESAEQEEEAHPEYKYGNELSADDLGHKEAIANSVKKGLVTIEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRLAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKASVTPQSSSELRPTTTAALGAGLEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKSTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSDMINTHSAEEQEIRDLHLSQQCELLKKLLINAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQAKEMQQMVKLEAEMDRRPATVV

Target ORF information:

RefSeq Version	XM_007452038.1
Organism	Lipotes vexillifer(Yangtze River dolphin)
Definition	Lipotes vexillifer phospholipase C, beta 4 (PLCB4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007452038.1

ORF Insert Sequence:

ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCGTTTTT GCAAGAAGGA 
GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAG 
GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG ACAAGTTCTA 
GAATGTTCTC TCATCAACAG TGTTCGACTG GGAGCCACAC CAAAGGATCC CAAAATTTTG 
GCTGCTCTTG AAGCTATTGG AAAGTCAGAA AATGACCTGG AAGGGCGGGT AGTTTGTGTC 
TGCAGTGGTA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA AAATTCAGAA 
GTAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATAC ACAACTTCAG GGCCAACAAT 
GTCAGTCCCA TGACATGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA 
AATGGGAAAA TTCCAGTTAG GAGTATTACC AGAACCTTTG CATCGGGGAA AACAGAAAAG 
GTGATCTTTC AAGCACTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAA 
CCTGCAGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA 
GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC 
CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GATTGAATGA AATTTTATTC 
CCATTTTATG ATACTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC TGATGAAGAC 
TTGAAGAAAA AAGGCCAAAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA 
AATGCCCCAG TCTTCCTAGA TCGCCTAGAG CTTTACCAAG AAATGGACCA CCCTCTGGCT 
CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGCAAG 
TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC 
TGTTGGGATG GAAAAGGTGA GGACCAAGAA CCAATAATAA CTCATGGAAA AGCAATGTGC 
ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCT 
GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG 
TCCAAATATT GCGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT 
CCACTGGAAC CAGGCAGGCC TTTGCCATCC CCAAATGACC TCAAAAGAAA AATACTCATC 
AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TTGAAGCTTT GAAAAGTATG 
ATGGAAGCAG GAGAATCCGC TGCCCCAGTG AACATCTTAG AGGATGACAA TGAAGAGGAA 
ATAGAAAGTG CTGAGCAAGA GGAAGAAGCT CACCCTGAAT ACAAATATGG AAATGAACTT 
TCTGCGGACG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTTAAGAA GGGCCTGGTA 
ACTATAGAAG ATGAGCAGGC ATGGATGGCA TCTTATAAAT ATGTAGGCGC CACCACTAAT 
ATCCATCCAT ATTTGTCCAC AATGATCAAC TATGCACAGC CCGTAAAGTT TCAAGGTTTC 
CACGTGGCTG AAGAACGCAA TATTCATTAT AACATGTCTT CTTTTAATGA GTCAGTTGGT 
CTAGGCTACT TGAAGACACA TGCGATTGAA TTTGTGAATT ATAACAAACG GCAAATGAGC 
CGCATTTACC CCAAGGGAGG CCGAGTCGAT TCCAGTAATT ACATGCCTCA GATTTTCTGG 
AACGCTGGCT GCCAGATGGT TTCGCTGAAC TATCAAACCC CAGATTTAGC GATGCAATTG 
AATCAGGGAA AATTTGAGTA TAATGGATCG TGCGGGTACC TTCTCAAACC AGATTTCATG 
AGACGGCCCG ATAGAACATT TGATCCCTTC TCTGAAACGC CTGTTGATGG GGTTATTGCA 
GCCACTTGCT CAGTGCAGGT CATATCAGGT CAGTTCTTAT CAGATAAAAA AATTGGCACA 
TATGTAGAAG TGGATATGTA TGGGTTGCCC ACCGACACTA TCCGGAAGGA ATTCCGAACA 
CGCATGGTGA TGAACAATGG ACTCAATCCA GTTTACAATG AAGAATCATT TGTATTTCGG 
AAGGTGATCC TCCCTGACCT GGCAGTCTTG AGACTTGCAG TGTATGATGA CAACAACAAG 
CTGATTGGCC AGAGGATTCT GCCACTGGAT GGCCTCCAAG CAGGCTATCG ACACATTTCT 
CTTCGAAATG AGGGAAATAA ACCTTTATCA CTGCCAACAA TTTTCTGCAA TATTGTTCTT 
AAAACATATG TGCCTGATGG ATTTGGAGAT ATTGTGGATG CTTTATCAGA TCCAAAGAAA 
TTTCTTTCCA TTACGGAAAA GAGAGCAGAC CAAATGAGAG CTATGGGCAT TGAAACTAGT 
GACATCGCCG ACGTGCCCAG TGACACGTCA AAGAATGACA AGAAAGGGAA AGCCAACACA 
GCGAAAGCAA GTGTGACCCC TCAGAGCAGC TCAGAGCTCA GGCCCACCAC CACGGCTGCC 
CTGGGAGCTG GCCTGGAGGC CAAGAAAGGT ATTGAACTGA TCCCTCAAGT GAGGATAGAA 
GATTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAACAGAA GGAGCTCAAT 
TCTTTAAAGA AGAAACATGC AAAGGAGCAC AGTACCATGC AGAAGCTACA TTGCACACAA 
GTTGACAAAA TTGTGGCACA GTATGACAAG GAGAAGTCAA CTCATGAGAA AATCCTAGAG 
AAGGCAATGA AGAAGAAGGG GGGAAGTAAT TGTCTTGAAA TGAAGAAAGA AACAGAAATT 
AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AGGAGATCGT GGCGCAGCAC 
ACAAAAGAGT GGTCAGACAT GATCAACACC CACAGTGCAG AGGAGCAGGA GATCCGGGAT 
CTGCACCTCA GCCAGCAGTG CGAGCTGCTC AAAAAGCTCC TGATCAACGC CCACGAGCAG 
CAGACCCAGC AGTTGAAACT GTCCCATGAC AGGGAAAGCA AGGAAATGCG AGCACACCAG 
GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCTAT CAAGAATAAA 
GCAGAACGGG AAAGGCGGGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA 
GAAAGAAAGC GACTCGCAAT GAAGCAGTCC AAAGAAATGG ACCAGTTGAA AAAAGTCCAG 
CTTGAACACC TCGAATTCCT AGAGAAACAG AACGAGCAGG CGAAGGAGAT GCAGCAGATG 
GTGAAATTGG AAGCCGAGAT GGACCGCAGA CCAGCAACAG TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PLCB4 cDNA ORF clone, Lipotes vexillifer(Yangtze River dolphin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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