PUF4 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	PUF4
Entrez Gene ID	10966080
Full Name	putative pumillio protein 4
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_003392229.1	XP_003392277.1	putative pumillio protein 4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PUF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc07443	XM_003392229.1 Latest version!	Leishmania infantum JPCM5 putative pumillio protein 4 (PUF4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PUF4 ( XM_003392229.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_003392277.1 Leishmania infantum JPCM5 putative pumillio protein 4 (PUF4), partial mRNA.

Price & Availability↑

CloneID	OLc07443
Clone ID Related Accession (Same CDS sequence)	XM_003392229.1
Accession Version	XM_003392229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative pumillio protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009396). COMPLETENESS: incomplete on both ends.

ATGTTCGCCC AATCCGAGAG CGAAGAGCTG CTGCATCTGC TGTCGCAGGT GCGCCTCGTG 
GCGGACGAGG AAGAGCGTAA CGAGCAAGAG CAGCATCAGC AAGAAAAACT GGAGCAGCGG 
CATCGCCCAA GCGTCGACGG TGAGCACACG ATGCCGGTGT ATACTTCTGC TGCCGGCGCG 
ACAGCGAAGG TACTTGACAA GCAGATAAGC AACGGCAGCT CGGCGAGCAT CGCCAGCGGC 
GGCGGCGGCT CTGCTGCAGG ACGATATACC GCCTCCTCCA CCGCGGCCGT CACGAGCAGC 
TGGACTGTGC ATGACAATAC GGACAGCACC TTTGGCATCA ACACCGCAGC GGCGAGCAAC 
AGCTTTCTCG CCGACTACGA GCACCACCGC GCGAACCCGT TTGACCAGCC GCCCGTGACT 
CGCACCCCGG CCACAGACGT GAAGGTGCTG CTGCAGCCGT CGGACGAGCG TGCAAGGTGC 
ATCGAGGTGG ACATGGCCAG CCGAGGCGAC GGCTACAACG TATTCCGGCA GCCGAAGCAG 
CTCCTCTTCG CCTCCCCATC AGCAGCGGCT CAGGTAGTGA CTGTGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGCGGCGGCG TCTCGAGCAG CTGCAGCAGC AGAGACCATT GTCACTAGTG 
ACGCACGCGC GCCAGTCCAT GCCCTCCCCC CACGCGTACA ACGTTTACAC CGCATCCGTG 
AGCACGGCAA CCAGAACGAC GACACCGCGC TCGATCGACG ATGCAGCGCC GATCTTGCCG 
GTTGCGGTCG AGCGCGAAGG CAGCGGCTAC CGCGGTGCTG CGGCCGATAG TGCGATACTT 
CGAGACACCC CCTCACCAGC TGTCACCTCA TCGGCACCAT CCGCCTACAC CAAGCGTGAT 
GGCAAGACGG TGGCGGAAGT AGTGCTAGAG GGCATCTCGG ACTTTGCAGC TGTGATACAG 
AATGATGGTT TCGACGGGGA TTGCGAGGAG GAGTTTGGCC GCACCAGTGG TGGCGGGGGT 
GGCGGTGACC GGCTATCGGA TGTCTGGACG CCCGCGCGCA GTATCGGCAC GCCGCAGTCA 
GCGCTAGATG GCTCTAACCG ATCGCTGCGC CTGTTTCACC TGCAGCAGCA GCAGCAGTCG 
CATCAACACC TTCACCAGCA CCACACCACC CCATCTTCTC CGTCCTCCGC GGCTGAGGCT 
GCTGTCGCGC AGTTCTTTCG TGTAAGCAGC AGTGTCGATG TCATGGACAA CGGTGGCCTC 
GACAGTGCGT GGCGAGCGAG TGTTGAGGCG GAGCTCGCGG AGCTCCAGCG CGGTGGACGA 
CTGTCCATGA ACAGCGCGGT GGTGCAGGGA ACGCCGATAT CGACGGTCTC GCCGCTTCCA 
CCAACGTCGA CTACACCGTA TCACCAGCTG CTCAGCCAAC TGCGGCACAG CCCGTCACAA 
GTGCCGGCGA GTGGGCTTGG GCCTGCCAAC ACTGCCTTCA CGGGCAGAGC GGCGCACGCG 
AATACGCCGC CGGACCGACG CCCGCCATCA TCATCCTCAC GCCTGGCCAG CATGGGGCCG 
ACGCCGCTCT ACACAACCCA CATCACGAGC ATACCGCGAG CTTCCTCCTC CGCTGCAGCC 
GCTGGCGCAT CGACGGAGCA GGGCCGCAGC TGCTGGCCGC GCTCCAGCCT CACCCCGTCC 
GAGGCGGCAG CTTTGTGGGA GCGCCTAAGT CCGTATGAGA GGGCCGCCGC CGCAGCAGCA 
GCAGCAGCGG CAAGCTCGAA CGCAGCCTCC GTCGCCGCAG CCGCGGCGCT GCTCCGCACC 
CCTGACACGG TTTCGAAGCC AGTCACGCTC TCCTCGATTG CTGCACACCT TCACAGCGGT 
GTTGGCGATC TGGGTAGCTC GGACGAAATG GGCGGAGCGC ACGCGCACGC GGACAATCCT 
TCTCGCACCT ATGCGTCCCC GCCAACGTTT ACCACGGCCC CGATGCGGGT GCCTCGCCCA 
GCCAGCGCGG CGCAGCTCTT CCCACCGTCG TCACCATCTC AGCCCAACTC TGCATCGCCA 
GCTACGGCCG GCACAGGCGT CTCGCCGCAC CACGGCTGGC AGCAAAGCCA CAGCAACCAT 
CGGCAGGGGC AGTATTCGCA GACAGCATCA CCTCGCACGA ATGCAACGAC GGTGATGGCC 
CTGCCACCAC CGCCACCGCC ACCACCTGCG TCGACGCCGT CACTGGCGAC AGCAGCAGCA 
GCAGCAGGCC CGTACAAATC CCAGCAGAAC CATTTGCAGA CGCCTTCGAT GCGCCGTTGG 
AGCCCCGTCG ACGCACCTCC ATCGACCACC GGTGTCGGCC CAGTCGCCGG TAACGCACAC 
AACACCAACT CACCTCCTTC CTGCAGTCTG CGTCCGTCGC CGCACCCTCG TCCGTCCAAC 
GGCAGCTCGC CGGCGCTGCG GCGATACACC ATCACCACTC CCAGCAGTCA CGCGACGACT 
GCGACAGCCG CCAACAACAG CACGAACAGT GGCGGCAAGA AGCACGTCGA TGGGAGCCCT 
CTCGGGGTCA GCGGTGCATC GGTCGCTGAC AAAGGAACGT CGCGCGAGGC GCGGCCACGT 
GTGCAGCTCT CGCAGACGTC CTGCTCGACT GGCGTCGCTG CCGCGGTCTG CGGCGGTGCT 
CGTGGGGCCC GCGGCTCGCT GGGGGCATCT GCACACGGCG CTGGCGGTGG TGCGAGCCCG 
AGCTCACCGT CATACCACCA CAACCACAGC AATCACGCCG GTCACCACCT GAACAGCCCC 
TCGAACTCCG CAGGCGCAAC CGCCGGGCCC GGGGCGTCCA TGGTGTCAGC CGCCGCCGTC 
CAAGCACTCA TCCAGCGCCT ACAGGCAGCC GCGGCGATCG TGCTGCCCAA CTCTCCGTCC 
GCGTCGTCCG CTGTGACGGG TGGCGGGCAG ATGTCTTTCA AGGCTGAGGA AAGCGGCGTC 
GCCAGCGTCG CCGCCGTGCC CGACGTTGCG TCGGCGCCGA TTATACTCGC CATGTCGCAC 
GATCAACACG GCTGCCGGCT TCTGCAGGCG GTGATCGACG CCGAGTGCAG CGAGGGCGAA 
GCGATTGACG GAGGGCACGA CGAGCTCGCG GCAGACGATG CCGCCGCCGC TGCGAGCCGG 
GCAGGGGCTG AGCGGGACGG GAGCGCCGCA GTCTCTCCGT CGATGACAGC CGGGGCGGGT 
GCTGGAGAGA AGAAGGGTAG CGTTAACGAG CGCCGCCGTC GTCGTCGCGC CGAAGCCTTT 
GAGAACTCCA TCCCTGTTCG CGTCGTTCTG CGGGCAATCG AACCCAAGCT GGACGCCGTC 
ATGGCCGACG GGTACGGCAA CTTCCTCCTC CAAAAAGTTT TCGACATGGC ACCTGATGCG 
GAGCGCCAAC GGCTGCTGCG GCTGCCGTCG CTGCAACATC ACCTGTGCGA GGTCGCCTGC 
TCTCCCCATG GCACCTTCGC GGTGCAGCGG CTCGTCGAGA CGGTTCGCAA CATGGAAGAG 
GAGCGCCTCG TTTTCGTCGC TCTGGAGCGT GATCTGCTGC GCCTGCTGAC GAACGCGAAC 
GGGGGCCACG TACTGATGAA GGTGATGGAG TGCATCCGGC GCCAGTATAA CGCCTTGGCA 
TCTGGCGCGA GCAGTGTGAC AGAGCCGTCA TCCGCGTCCA TGGCGGCGCC GGGCGAAAGC 
AGCACCGCAC CATCACGCCG CCTGCTCGAG GAAAGGGTGG CGACACTTTT TCAGGCGATT 
CAGCAGCATC TGCTGTACGT CTGCCAGCAC AAGCAGGGTT GCTGCATTGT CCAGAAGTGC 
CTGGACTTCC TCCACGCGTG CGCTGGATTA TCGCCGTCGT TGCCGTCATC CTCTGGCGCG 
GTGGAGCAGA TGGACTACTT TGAACGCATG GCAGCGCTTC TCCTTCCCCA CGTGCAGGAG 
CTCTCCACTC ACCCGTTTGG CAACTACGTC GTGACGCGCC TCGTGGACGT CTGCTACGCG 
CGCGGGAGCA CCGCCACCAT CGACGCCGTG GCGGCCGGCA TGCAGCGGGA CTTGGTGCAG 
ATGTGCACGA ACAAGTTTGC ATCAAATGTG GTTGAGCACA TTCTGCGCCA CTGCAGCGAG 
CGGCGCATTC GCCTCATCTG CCAGGCGCTG ATGATGCCCC TCGCCCACCC CTCTGCACCA 
GCGGTGTACG CCTCTGCCTC GACAGCGGCG GCCACAAACG CCGCCATCGC TCGGCTGCCG 
TTGATCATCG TTGTCATGGA CCCCTATGGC AACTATGTGA TCCAGACGCT GCTGACGGTG 
GCGCCGGTGG ACGAGCTGGT GAGCACGCGC GCAGAGGGAG GCGGCATGCT ACCCGTGCTT 
CAGCAGCTTC TCCCGCTGCT CAGCTCACGC AACTACGGCC GGAAGCTGGA GACGAAGACG 
GAGCTCGCCT TGCTGAGGGT GGAGCAGCAC CAACAGCAGC AGAAGCAGCG CCGCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003392277.1
CDS	1..4440
Translation	MFAQSESEELLHLLSQVRLVADEEERNEQEQHQQEKLEQRHRPSVDGEHTMPVYTSAAGATAKVLDKQISNGSSASIASGGGGSAAGRYTASSTAAVTSSWTVHDNTDSTFGINTAAASNSFLADYEHHRANPFDQPPVTRTPATDVKVLLQPSDERARCIEVDMASRGDGYNVFRQPKQLLFASPSAAAQVVTVQQQQQQQQQRRRLEQLQQQRPLSLVTHARQSMPSPHAYNVYTASVSTATRTTTPRSIDDAAPILPVAVEREGSGYRGAAADSAILRDTPSPAVTSSAPSAYTKRDGKTVAEVVLEGISDFAAVIQNDGFDGDCEEEFGRTSGGGGGGDRLSDVWTPARSIGTPQSALDGSNRSLRLFHLQQQQQSHQHLHQHHTTPSSPSSAAEAAVAQFFRVSSSVDVMDNGGLDSAWRASVEAELAELQRGGRLSMNSAVVQGTPISTVSPLPPTSTTPYHQLLSQLRHSPSQVPASGLGPANTAFTGRAAHANTPPDRRPPSSSSRLASMGPTPLYTTHITSIPRASSSAAAAGASTEQGRSCWPRSSLTPSEAAALWERLSPYERAAAAAAAAAASSNAASVAAAAALLRTPDTVSKPVTLSSIAAHLHSGVGDLGSSDEMGGAHAHADNPSRTYASPPTFTTAPMRVPRPASAAQLFPPSSPSQPNSASPATAGTGVSPHHGWQQSHSNHRQGQYSQTASPRTNATTVMALPPPPPPPPASTPSLATAAAAAGPYKSQQNHLQTPSMRRWSPVDAPPSTTGVGPVAGNAHNTNSPPSCSLRPSPHPRPSNGSSPALRRYTITTPSSHATTATAANNSTNSGGKKHVDGSPLGVSGASVADKGTSREARPRVQLSQTSCSTGVAAAVCGGARGARGSLGASAHGAGGGASPSSPSYHHNHSNHAGHHLNSPSNSAGATAGPGASMVSAAAVQALIQRLQAAAAIVLPNSPSASSAVTGGGQMSFKAEESGVASVAAVPDVASAPIILAMSHDQHGCRLLQAVIDAECSEGEAIDGGHDELAADDAAAAASRAGAERDGSAAVSPSMTAGAGAGEKKGSVNERRRRRRAEAFENSIPVRVVLRAIEPKLDAVMADGYGNFLLQKVFDMAPDAERQRLLRLPSLQHHLCEVACSPHGTFAVQRLVETVRNMEEERLVFVALERDLLRLLTNANGGHVLMKVMECIRRQYNALASGASSVTEPSSASMAAPGESSTAPSRRLLEERVATLFQAIQQHLLYVCQHKQGCCIVQKCLDFLHACAGLSPSLPSSSGAVEQMDYFERMAALLLPHVQELSTHPFGNYVVTRLVDVCYARGSTATIDAVAAGMQRDLVQMCTNKFASNVVEHILRHCSERRIRLICQALMMPLAHPSAPAVYASASTAAATNAAIARLPLIIVVMDPYGNYVIQTLLTVAPVDELVSTRAEGGGMLPVLQQLLPLLSSRNYGRKLETKTELALLRVEQHQQQQKQRRS

Target ORF information:

RefSeq Version	XM_003392229.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative pumillio protein 4 (PUF4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003392229.1

ORF Insert Sequence:

ATGTTCGCCC AATCCGAGAG CGAAGAGCTG CTGCATCTGC TGTCGCAGGT GCGCCTCGTG 
GCGGACGAGG AAGAGCGTAA CGAGCAAGAG CAGCATCAGC AAGAAAAACT GGAGCAGCGG 
CATCGCCCAA GCGTCGACGG TGAGCACACG ATGCCGGTGT ATACTTCTGC TGCCGGCGCG 
ACAGCGAAGG TACTTGACAA GCAGATAAGC AACGGCAGCT CGGCGAGCAT CGCCAGCGGC 
GGCGGCGGCT CTGCTGCAGG ACGATATACC GCCTCCTCCA CCGCGGCCGT CACGAGCAGC 
TGGACTGTGC ATGACAATAC GGACAGCACC TTTGGCATCA ACACCGCAGC GGCGAGCAAC 
AGCTTTCTCG CCGACTACGA GCACCACCGC GCGAACCCGT TTGACCAGCC GCCCGTGACT 
CGCACCCCGG CCACAGACGT GAAGGTGCTG CTGCAGCCGT CGGACGAGCG TGCAAGGTGC 
ATCGAGGTGG ACATGGCCAG CCGAGGCGAC GGCTACAACG TATTCCGGCA GCCGAAGCAG 
CTCCTCTTCG CCTCCCCATC AGCAGCGGCT CAGGTAGTGA CTGTGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGCGGCGGCG TCTCGAGCAG CTGCAGCAGC AGAGACCATT GTCACTAGTG 
ACGCACGCGC GCCAGTCCAT GCCCTCCCCC CACGCGTACA ACGTTTACAC CGCATCCGTG 
AGCACGGCAA CCAGAACGAC GACACCGCGC TCGATCGACG ATGCAGCGCC GATCTTGCCG 
GTTGCGGTCG AGCGCGAAGG CAGCGGCTAC CGCGGTGCTG CGGCCGATAG TGCGATACTT 
CGAGACACCC CCTCACCAGC TGTCACCTCA TCGGCACCAT CCGCCTACAC CAAGCGTGAT 
GGCAAGACGG TGGCGGAAGT AGTGCTAGAG GGCATCTCGG ACTTTGCAGC TGTGATACAG 
AATGATGGTT TCGACGGGGA TTGCGAGGAG GAGTTTGGCC GCACCAGTGG TGGCGGGGGT 
GGCGGTGACC GGCTATCGGA TGTCTGGACG CCCGCGCGCA GTATCGGCAC GCCGCAGTCA 
GCGCTAGATG GCTCTAACCG ATCGCTGCGC CTGTTTCACC TGCAGCAGCA GCAGCAGTCG 
CATCAACACC TTCACCAGCA CCACACCACC CCATCTTCTC CGTCCTCCGC GGCTGAGGCT 
GCTGTCGCGC AGTTCTTTCG TGTAAGCAGC AGTGTCGATG TCATGGACAA CGGTGGCCTC 
GACAGTGCGT GGCGAGCGAG TGTTGAGGCG GAGCTCGCGG AGCTCCAGCG CGGTGGACGA 
CTGTCCATGA ACAGCGCGGT GGTGCAGGGA ACGCCGATAT CGACGGTCTC GCCGCTTCCA 
CCAACGTCGA CTACACCGTA TCACCAGCTG CTCAGCCAAC TGCGGCACAG CCCGTCACAA 
GTGCCGGCGA GTGGGCTTGG GCCTGCCAAC ACTGCCTTCA CGGGCAGAGC GGCGCACGCG 
AATACGCCGC CGGACCGACG CCCGCCATCA TCATCCTCAC GCCTGGCCAG CATGGGGCCG 
ACGCCGCTCT ACACAACCCA CATCACGAGC ATACCGCGAG CTTCCTCCTC CGCTGCAGCC 
GCTGGCGCAT CGACGGAGCA GGGCCGCAGC TGCTGGCCGC GCTCCAGCCT CACCCCGTCC 
GAGGCGGCAG CTTTGTGGGA GCGCCTAAGT CCGTATGAGA GGGCCGCCGC CGCAGCAGCA 
GCAGCAGCGG CAAGCTCGAA CGCAGCCTCC GTCGCCGCAG CCGCGGCGCT GCTCCGCACC 
CCTGACACGG TTTCGAAGCC AGTCACGCTC TCCTCGATTG CTGCACACCT TCACAGCGGT 
GTTGGCGATC TGGGTAGCTC GGACGAAATG GGCGGAGCGC ACGCGCACGC GGACAATCCT 
TCTCGCACCT ATGCGTCCCC GCCAACGTTT ACCACGGCCC CGATGCGGGT GCCTCGCCCA 
GCCAGCGCGG CGCAGCTCTT CCCACCGTCG TCACCATCTC AGCCCAACTC TGCATCGCCA 
GCTACGGCCG GCACAGGCGT CTCGCCGCAC CACGGCTGGC AGCAAAGCCA CAGCAACCAT 
CGGCAGGGGC AGTATTCGCA GACAGCATCA CCTCGCACGA ATGCAACGAC GGTGATGGCC 
CTGCCACCAC CGCCACCGCC ACCACCTGCG TCGACGCCGT CACTGGCGAC AGCAGCAGCA 
GCAGCAGGCC CGTACAAATC CCAGCAGAAC CATTTGCAGA CGCCTTCGAT GCGCCGTTGG 
AGCCCCGTCG ACGCACCTCC ATCGACCACC GGTGTCGGCC CAGTCGCCGG TAACGCACAC 
AACACCAACT CACCTCCTTC CTGCAGTCTG CGTCCGTCGC CGCACCCTCG TCCGTCCAAC 
GGCAGCTCGC CGGCGCTGCG GCGATACACC ATCACCACTC CCAGCAGTCA CGCGACGACT 
GCGACAGCCG CCAACAACAG CACGAACAGT GGCGGCAAGA AGCACGTCGA TGGGAGCCCT 
CTCGGGGTCA GCGGTGCATC GGTCGCTGAC AAAGGAACGT CGCGCGAGGC GCGGCCACGT 
GTGCAGCTCT CGCAGACGTC CTGCTCGACT GGCGTCGCTG CCGCGGTCTG CGGCGGTGCT 
CGTGGGGCCC GCGGCTCGCT GGGGGCATCT GCACACGGCG CTGGCGGTGG TGCGAGCCCG 
AGCTCACCGT CATACCACCA CAACCACAGC AATCACGCCG GTCACCACCT GAACAGCCCC 
TCGAACTCCG CAGGCGCAAC CGCCGGGCCC GGGGCGTCCA TGGTGTCAGC CGCCGCCGTC 
CAAGCACTCA TCCAGCGCCT ACAGGCAGCC GCGGCGATCG TGCTGCCCAA CTCTCCGTCC 
GCGTCGTCCG CTGTGACGGG TGGCGGGCAG ATGTCTTTCA AGGCTGAGGA AAGCGGCGTC 
GCCAGCGTCG CCGCCGTGCC CGACGTTGCG TCGGCGCCGA TTATACTCGC CATGTCGCAC 
GATCAACACG GCTGCCGGCT TCTGCAGGCG GTGATCGACG CCGAGTGCAG CGAGGGCGAA 
GCGATTGACG GAGGGCACGA CGAGCTCGCG GCAGACGATG CCGCCGCCGC TGCGAGCCGG 
GCAGGGGCTG AGCGGGACGG GAGCGCCGCA GTCTCTCCGT CGATGACAGC CGGGGCGGGT 
GCTGGAGAGA AGAAGGGTAG CGTTAACGAG CGCCGCCGTC GTCGTCGCGC CGAAGCCTTT 
GAGAACTCCA TCCCTGTTCG CGTCGTTCTG CGGGCAATCG AACCCAAGCT GGACGCCGTC 
ATGGCCGACG GGTACGGCAA CTTCCTCCTC CAAAAAGTTT TCGACATGGC ACCTGATGCG 
GAGCGCCAAC GGCTGCTGCG GCTGCCGTCG CTGCAACATC ACCTGTGCGA GGTCGCCTGC 
TCTCCCCATG GCACCTTCGC GGTGCAGCGG CTCGTCGAGA CGGTTCGCAA CATGGAAGAG 
GAGCGCCTCG TTTTCGTCGC TCTGGAGCGT GATCTGCTGC GCCTGCTGAC GAACGCGAAC 
GGGGGCCACG TACTGATGAA GGTGATGGAG TGCATCCGGC GCCAGTATAA CGCCTTGGCA 
TCTGGCGCGA GCAGTGTGAC AGAGCCGTCA TCCGCGTCCA TGGCGGCGCC GGGCGAAAGC 
AGCACCGCAC CATCACGCCG CCTGCTCGAG GAAAGGGTGG CGACACTTTT TCAGGCGATT 
CAGCAGCATC TGCTGTACGT CTGCCAGCAC AAGCAGGGTT GCTGCATTGT CCAGAAGTGC 
CTGGACTTCC TCCACGCGTG CGCTGGATTA TCGCCGTCGT TGCCGTCATC CTCTGGCGCG 
GTGGAGCAGA TGGACTACTT TGAACGCATG GCAGCGCTTC TCCTTCCCCA CGTGCAGGAG 
CTCTCCACTC ACCCGTTTGG CAACTACGTC GTGACGCGCC TCGTGGACGT CTGCTACGCG 
CGCGGGAGCA CCGCCACCAT CGACGCCGTG GCGGCCGGCA TGCAGCGGGA CTTGGTGCAG 
ATGTGCACGA ACAAGTTTGC ATCAAATGTG GTTGAGCACA TTCTGCGCCA CTGCAGCGAG 
CGGCGCATTC GCCTCATCTG CCAGGCGCTG ATGATGCCCC TCGCCCACCC CTCTGCACCA 
GCGGTGTACG CCTCTGCCTC GACAGCGGCG GCCACAAACG CCGCCATCGC TCGGCTGCCG 
TTGATCATCG TTGTCATGGA CCCCTATGGC AACTATGTGA TCCAGACGCT GCTGACGGTG 
GCGCCGGTGG ACGAGCTGGT GAGCACGCGC GCAGAGGGAG GCGGCATGCT ACCCGTGCTT 
CAGCAGCTTC TCCCGCTGCT CAGCTCACGC AACTACGGCC GGAAGCTGGA GACGAAGACG 
GAGCTCGCCT TGCTGAGGGT GGAGCAGCAC CAACAGCAGC AGAAGCAGCG CCGCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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PUF4 cDNA ORF clone, Leishmania infantum JPCM5

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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