SPG11 cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPG11
Entrez Gene ID	100070629
Full Name	SPG11, spatacsin vesicle trafficking associated
General protein information	Preferred Names SPG11, spatacsin vesicle trafficking associated Names LOW QUALITY PROTEIN: spatacsin spastic paraplegia 11 (autosomal recessive)
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014733570.1	XP_014589056.1	LOW QUALITY PROTEIN: spatacsin
XM_023618492.1	XP_023474260.1	spatacsin isoform X1
XM_023618503.1	XP_023474271.1	spatacsin isoform X2
XM_023618497.1	XP_023474265.1	spatacsin isoform X2

Pathways from BioSystems
Gene Ontology

The following SPG11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPG11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHb28607	XM_014733570.1 Latest version!	Equus caballus spastic paraplegia 11 (autosomal recessive) (SPG11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHb42160	XM_023618492.1 Latest version!	Equus caballus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHb42161	XM_023618503.1 Latest version!	Equus caballus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb42161	XM_023618497.1 Latest version!	Equus caballus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPG11 ( XM_014733570.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014589056.1 Equus caballus spastic paraplegia 11 (autosomal recessive) (SPG11), mRNA.

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CloneID	OHb28607
Clone ID Related Accession (Same CDS sequence)	XM_014733570.1
Accession Version	XM_014733570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1447862400000
Organism	Equus caballus(horse)
Product	LOW QUALITY PROTEIN: spatacsin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867387.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCTGGTT GCCGCAATAT GAAGATCATT GAGGACCTTG AGGTGGACAG GGACGCAGTT 
CAGCAGCTTT CCCCGGGAGG CACGGGTCTG CGAGCGGGTC TGCTACACTG CGCATGCCCA 
GGATGCAGGC GGGGTCCGGC GCGGGTGACC GGAAGAAACC GCCGAGCCAC GTTGGCCGCA 
GAGCAGGCGG CCGGAAGTGC CGCTGCCGGC GGTAGCTGCA ACGCTGCAGC CATGGAGCGG 
GTTCTGCCTA TGTTGTTGGT GCCAGTCCCC GCCGAGACAA CGGGGCGGCT GGGGGCCCGG 
GCGCAGCTGC ACAGAGAGCA GGAGGTTCTG GGGAGCCTGA CGGCCTCGGG CTGCCTTCAA 
GTGCTCTCCT TGGCGCCCGG AGGCCGGGGC GGGGGCGGCT GCCGCCTGGA TGGCCCCTTT 
CGGCATTTTG TGTGGGAGGA TTCTCATAAC AGCAGCGCAC CAACAGACAA GCCCAGACTG 
CTCGCTCTTA CTGACAATTA TGAACTGCTC GTCTATGAAT TTAATTTGAA AGATGGAAGA 
TGTGATGCAA CCATTTTGTA TAGCTATAGT GAGGAGAGAT TGCAAAAGCT CGTTGAAGAT 
CTAAATATCA GTATTTCCTT ATTATCTTTG AGAATTCTGT CATTTCACAA TAACACATCA 
TTATTGTTCA TCAACAAGTG TATCATCCTG CAGGTTGTAT TTCTTGAAAG AGATACTGAG 
ATTAGAGTAC TCAACTGTTT TACATTACCT CTGCCTACAC AGGCAGTGGA CAGGATTATT 
GACACGCAGC TCTGCAGAGG AATTCTCTTT GTTTTGAGTA GTTTAGGCTG GATCTATATT 
TTTGACATTG TGGACGGTAC ACATGTAGCT CATGTGGATT TAGCACTTCA CCAAGAAGAT 
ATGTGTAATG AGCAACAACA GGAGCCAGCC AAGATTTCTT CTTTTACTTC ACTGAAAGTG 
TCTCAAGACC TAGATGTTGT AGTGATTGTC AGCTCCTCCA ACTCTGCAAT TGCTCTTAAC 
TTAAATTTGT ATTTCAGGCA ACACCCAGGA CACCTACTAT GCGAAAGAAC TCTAGAAGAC 
AGTCCTATTG AAGGCCCTAA GGAGATAGAT GAAGATGATC CTGCTAATTC TGACTACAAC 
ATGAAACTGA CCAAGTTTTC CTTCCAAGTT GATAGGTCTT GGAAAGCCCA GCTATCATCA 
TTGAATGAAA CAATAAAGAG CTCCAAGCTG GAGGTTTCCC ATTGTGCTCC ATGGTTCCAG 
GATATTTTAC ACGTGGAGCC TTCTGAATCT GGTAACCACG GTACCAGTGT GCCAGGCTGG 
GCCTTCATTC CACAGGACAT AATGGGCCGC CAGTATAATT TTCCACAGAA AGATCACACC 
AAGACCAATG ATCCAGGAAG ATCCTGGAAA GTAGTGCACA TCAATGAACA AGAGGAACCC 
ATCAAGCTTA AATGTGTGTC TGTGACAGGA TTCACTGCAC TGTTTACTTG GGAAGCAGAG 
AGGATGGGCT GCACCATTGT CCTTTGGGAT TTGGAGACCC AGGGCATGCA GTGTTGTCCT 
CTTGGCAAAA AATGTATTCC TGTAGACAGC AGTGGAGACC AGCAGCTGTG CCTTGTTTTG 
ACAGAAAGTG GACTTTCCCT GATTTTGTTT GGTTTGACTC AAGAGGAGTT TTTAAATAGA 
CTAATGATCC ATGGAAGTGC CAGCACTGTG GACTCTCTCT GCCATCTCAA TGGTTGGGGG 
AGGTGCTCAA TCCCCATACA TGCACTAGAG GCTGGCATAG AAAATCGTCA GCTGGACACA 
GTAGATTTCT TTTTGAAGAG CAAGGAAAAT CTTTTGAATC CATCCTCAAA ATCTTCTGTA 
GCTGATCAGC TTGACCACTT TCCATCCCAT TTGTATTTAA GAAATGTGGA AGAGCTGACA 
CCAGCATTGG ATTTACTTTG CTCAGCAATT AGAGAAAGTG ATTCTGAAAC CCAGAGCAAA 
CACTTCTCAG AACAGTTGCT TAATCTTACA CTGTCTTTCC TTAACAAACA AATAAAGGAG 
CTTTTTATTC ACAATGAAGA GCTAGATGAA CATCTACAGA AGGGAGTGAA CATTTTGACC 
AGCTACATTA ATGAACTTCG AACCTTCATG ATAAAGTTTC CTTGGAAGCT AACAGATGCC 
ATAGATGAAT ATGATGCAAA TGAAAATGTC CCCAAAGTAA AGGAGAGCCA TATGTGGGAG 
AAACTCAGCG TTGAGGAAGT TATCACCAAT GCCGTTTTAA ACAACAAAAT ACCAGAGGCA 
CAAACTTTCT TTAGGATTAA CAGTCATTCT GCTCAAAGAC TTGAGGAGCT GATTAGAATA 
GGCCTAGATT TGGTCTTTGA CAGTTTGAAA AAGAATAATA TAAAGGAAGC CTCCGAACTT 
TTGAAGAATA TGGGCTTTGA TGTAAAAGAC CAGCTGCTCA AGATATGCTT CTATACGACT 
GATAAAAATA TACGGGACTT TTTGGTTGAA ATTTTAAAAG AAAAAAATTA TTTTTCTGAA 
AAAGAAAAAA CAACTATAGA CTTTGTGCAT CGAGTTGAGA AGTTTTATTC AGGACATTTC 
CAAGAAAATG TGCAGATCCA GTCCTTTCCC AGATATTGGA TAAAGGAAGA AGATTTTTTC 
AAGCACAAAT CTGTTTTGGA CTCATTCCTG AAACATGACC ATAAAGATAA ATTTAACAGA 
CAGGATCATA GAATTGTGTT AAATTGGGCC CAGTGGTGGA ATCGAATAAC ACAAGAATCC 
ATCCTTCTCC CCAGGATAAG TCCAGAAGAA TACAAATCAT GTTCTCCTGA AGCCCTCTGG 
AGGTACCTCA CAGCTCGCCA TGATTGGTTA AACATTGTCA CGTGGATTGG AGAATTTCAG 
ACCCAGAGTA GTGATTCTTC ACTTCAGCAG CACAAGTGGC CCCCTCTGAC TGTTGATGTT 
ATTGACCACA ACACTTGCTG CAGCAACTAC ATGAGGAATG AAATTTTAGA TAAGTTGGCC 
AGGAATGGGA TTTTTCTTAG ATCTGAACTA GAAGACTTTG AACTCTTCCT CTTAAGACTG 
AACCGTATTG GGGGTGTGAT GCAAGATACC CTCCCTGTTC AAAACTACAG GTCCAAAGAA 
GGTTGGGACT TCCATTCTCA CTTCATTCTC TATTGTTTGG AACGCAGTCT GCAGTATCTT 
CTTTATGTCT ATCTAGACTG TTACAAACTT AGTCCTCCTA ATTGTCCTTT TTTGGAAAGA 
AAAGAATTAC ATGAAGCTCA CCCGTGGTTT GAATTTTTAG TTCAGTGTCG ACAAGTTTCC 
AGTAATTTAA CAGATCCCAA ATTAATCTTC CAGGCTAGCC TTGCAAATGC TCAGATTCTG 
ATTCCCAGCA ACCAGGCCAG TGTGAGCAGT ATGCTATTGG AAGGACATAC CCTCCTGGCC 
CTTGCTACCA CAATGTATGC TCCTGGGGGT GTCAGCCAGG TTGTTCAGAA TGAAGAAAAT 
GAGAACTGTT TGAAGAAAGT AGACCCCCAG CTACTGAAGA TGGCATTAAC TCCTTACCCC 
AAGCTTAAAA CTGCTCTCTT CCCACAGTAC ACTGCCCCTA GTGTCCTGCC ACCCGACATT 
ACACTCTACC ACCTTCTTCA GTCGTTATTG CCCTTTGATC CCAGCAGATT GTTTGGCTGG 
CAGTCTGCCA ACACACTAGC TATAGGAGAT GCATCGAGTC ACCTCCCCCA TTTCTCTAGC 
CCAGACCTGG TTAATAAGTA TGCTATCGTG GAACGTCTGA ATTTTGCTTA TTATTTACAT 
CATGGACGGC CATCATTTGC ATTTGGTACT TTTCTGGTCC AGGAATTANA CCAGAGCAAG 
ACTCCCAAGC AGCTGATCCA GCAAGTAGGC AATGAAGCCT ATGTTTTAGG CCTCTCCTCC 
TTCCATGTAC CTTCAATAGG AGCTGCCTGT GTTTGTTTCT TGGAATTGCT TGGCCTCGAC 
AGCCTCAAGC TCAGAGTTGA TATGAAAGTG GCCAATATAA TTTTGAGCTA CAAGTGTGGA 
AATGAAGATG CTCAGTACAG CTTTATCAGG GAGTCTCTAG CTGAAAAACT ATCTAAACTG 
GCTGCGGGTG AAAAGGCAAC CACAGAAGAA TTGCTTATTC TCTTAGAAGA GGGTATATGG 
AACAGCATTC AGCAGCAGGA AACAAAGAGA TTATCCAGTG AATCCAGCAG CCAATGGGCA 
TTAGTGGTAC AATTCTGCAG GCTCCACAAT GTGAAACTGA GCACGTCTTA CCTTATAGAG 
TGTGCCAAAG CAAATGACTG GTTACAGTTC ATTATTCATA GCCAGCTCCA TAATTACCAC 
CCAGAGGAGG TGAAATCTCT TCTCCAGTAT TTCAGCCCTG TCCTTCAAGA CCACTTACAG 
CTGGCTTTTG AGAACTTGCC CTCAGTGTCC AACTCCAGAA TGGACAATGA TCAAGTCTGC 
AACAGGTCCC CCCAGGAACT CCAGAGAAAC AAAGAAGATA CGACTGATTT CTTTAAAATT 
CTGCTGCAGT GCTCAGAGGA GCCAAACTCC TGGTGTTGGC TCCTAGCTGA AGCAGTGAAA 
CAACAGGCCC CTATCCTCAG TGTCCTGGCC TCGTGTCTCC AGGATTCCAG TGCTATTCCT 
TGTCTCTGTG TTTGGATCAT CACTTCTGTG GAGGACAGTG TTGCAGCTGA AGCAATGGGA 
CAAATTCAGG GTTCAACGGA GGGCCACACT TGGGACCTTG AGGACCTCTC TGTCATCTGG 
AGGACATTAT TAACGAGACA GAAGAGCAAA ACTCTCATCA GAGGTTTCCA ACTTTTCTTT 
AAGGATTCCC CGTTGCTATT GATGATGGAA ATGTATGAAC TGTGTATGTT CTTCAAGAAT 
TATAAGGAAG CCAAAGCAAA ACTATTGGAG TTCCAGAAGA ATCTTGAAAC TCTTGAAACA 
GCAGCCACAA AGGTCCACAC TGTCATCCCT GCCCCATGGC TGAAGGAGCA GGTGTGCTTT 
CTTTTGAAGC TTATGCTGCA GCAGTGTAGG ACCCAGTATG AGCTGGGGAA GCTTTTACAG 
CTGTTTGTTG GAGTAGAGCA TCTCTTCCCT GATGGTCCAG ATGTGAAGAA GCTGTGTGTC 
CTCAGCCAGA TTTTGAAAGA TACGTCCATA GCCATTAATC ATGTAATTAT TACCAGCTAC 
AGCCTTGAGA CTTTTCAGCA TGAATGTAGA TCTATCTTGG AAAAACTGGA GACAGATGGA 
CAGTTTGCTT TGGCCAGGAG AGTAGCAGAA TTAGCTGAGT TACCTGTGGA CAACTTGGTT 
ATTGAAGAGA TAACACAGGA AATGCAGACC TTAAAACACA TTGACCAGTG GTCTCTGAAA 
CAAGCAAAAA TTGACTTCTG GAAAAAATGC CATGAGAATT TTAAGAAAAA TTCCATTTCA 
AGCAAAGCAG CTTCTTCCTT TTTCTCAGCC CAGGCCCTTG TGGCATGTGC GTACCCGACT 
GAGGAGGGGC AGAGCAGCGT TGAGGAGCGC CATCTGCTGC TCACCCTGGC AGGGCACTGG 
CTTGCTCAGG AGGACCCGGT GCCTGTCAAC CAGCTGGAGG AGCTGGAGAA GCAGATCTGG 
CTGTGCCGCA TCACCCAGCA CACCCTCAGA GGAAGCCAGG GGGAAACAGA GCCCAGATTT 
TCTCAACAGA TCTCAACTAG TGGGGAACTT TCTTTTGATA GCTTAGCCAG TGAGTTTTCA 
TTCTCCAAGT TGGCTGCTCT GAACACATCA AAATACTTAG AACTTAATGG CCTTCCATCC 
AAAGAGACTT GTGAGAACAG ACTGGATTGG AAGGAGCAGG CGTCACTACA CTTTTTGATT 
GGGCGCCTCT TGGATGATGG CTGTGTGCAT GAAGCAAGTA GAGTGTGCCG GTATTTTCAT 
TTCTACAACC AAGATGTTGT CTTGGTATTG CACTGTAGAG CACTGGCTTC AGGTGAAGCT 
GGTGTGGATG ACCTGCATCC AGAGATCCAT GCTCTCCTAC AAAGTGCTGC GCTGCCTGAG 
GGAGAAGAAG AACCTGGCAT TCCTCTAAGG AAAGCCCAAA GCACTTCAAG TTTGGATAGT 
CAGTCGTTTG TGATGCTGCC CTCAAGTGAT GAAGTGGTGA CTAATCTGAA AACTCTGACT 
GGCAAATGCC TCCATGGGAA AAACTACTGC CGGCAGGTCC TCTGTCTGTA TGAACTTGCC 
AAGGAGTTGG GTTGTTCTTA CACAGATGTG GCTGCCCAGG ATGGTGAGGC CATGCTCCGG 
ACAATCTTGG CCTCTCAGCA GCCTGACCGG TGCAAGCGAG CCCAAGCCTT CATCAGCACC 
CAGGGCCTCG AGCCAGACAC GGTGGCTGAA CTTGTGGCCG AGGAGGTGAC CAGAGAGCTG 
CTGACCCCAT CAGAAAGAGC AGGACAGAAG CAGATGTTCA CCCCAGCAGA GGAAAGCCAG 
ACTTTTCTTC CACTGACCAC TTTGTGTGCA GACCGCACAT TGGTAGGCAT GAAGTTGTTG 
GATAAGATTT CCTCCGTTCC CCATGGGGAG TTGTCTTGCA CCACAGAGCT GCTGATCCTG 
GCCCATCACT GCTTCACGCT GACATGCCAC ATGGAGGGCA TCATCCGAGT CCTGCAGGCC 
GCCCGGCTGC TCACAGACAA CCACTTGGCC CCCAATGAAG AGTACGGGCT GGTGGTGCGT 
CTCCTCACTG GCATTGGAAG GTACAATGAG ATGACATACA TATTTGATTT GCTGCATAAA 
AAGCACTACT TTGAAGTGCT GATGAGGAAG AAATTAGATC CGAGTGGTAC CCTGAAGACA 
GCCCTACTCG ACTACATCAA ACGCTGCCGC CCTGGGGACA GTGAGAAGCA CAACATGATT 
GCCTTGTGCT TCAGCATGTG CCGTGAGATT GGGGAGAACC ATGAGGCAGC TGCCTGCATC 
CAGCTGAAAT TGATCGAGTC TCAGCCCTGG GAGGACAGCC TCAAAGATGG GCACCAACTG 
AAGCAGCTGC TGCTGAAGGC CCTGACTCTG ATGCTGGATG CAGCAGAGAG TTATGCCAAG 
GACTCTTGCG TGCGACAGGC CCTGCACTGT CATCGGCTCA CCAAGTTGAT AACACTGCAG 
ATTCACTTTC TGAACACTGG CCAGAACACA ATGCTCATCA ACCTGAGCCG CCACAGGCTG 
ATGGACTGTA TCATGACCCT ACCTCGGTTC TACCAGGCTT CTATTGTGGC GGAGGCCTAT 
GACTTTGTTC CTGATTGGGC TGAAATTCTA TACCAACGAG TGATTCTTAA AGGAGACTTT 
AATTACTTGG AAGAGTTTAA GCAGCAAAGG TTATTAAAAT CCAATATATT TGAAGAGATT 
TCAAAAAAAT ATAAACAACA TCAGCCTACC GACACAGTTG TGAAAAACCT GAAGAAGTTA 
CTCACGTATT GTGAAGATGT CTACTTGTAT TACAAACTAG CTTATGAACA CAAATTTTGT 
GATATTGTAA ATGTGCTTCT GAAGGACCCT CAGATAGGGT GCTGTTTAAA GGACATGCTA 
GCAGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014589056.1
CDS	1..7509
Translation	MAGCRNMKIIEDLEVDRDAVQQLSPGGTGLRAGLLHCACPGCRRGPARVTGRNRRATLAAEQAAGSAAAGGSCNAAAMERVLPMLLVPVPAETTGRLGARAQLHREQEVLGSLTASGCLQVLSLAPGGRGGGGCRLDGPFRHFVWEDSHNSSAPTDKPRLLALTDNYELLVYEFNLKDGRCDATILYSYSEERLQKLVEDLNISISLLSLRILSFHNNTSLLFINKCIILQVVFLERDTEIRVLNCFTLPLPTQAVDRIIDTQLCRGILFVLSSLGWIYIFDIVDGTHVAHVDLALHQEDMCNEQQQEPAKISSFTSLKVSQDLDVVVIVSSSNSAIALNLNLYFRQHPGHLLCERTLEDSPIEGPKEIDEDDPANSDYNMKLTKFSFQVDRSWKAQLSSLNETIKSSKLEVSHCAPWFQDILHVEPSESGNHGTSVPGWAFIPQDIMGRQYNFPQKDHTKTNDPGRSWKVVHINEQEEPIKLKCVSVTGFTALFTWEAERMGCTIVLWDLETQGMQCCPLGKKCIPVDSSGDQQLCLVLTESGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLNPSSKSSVADQLDHFPSHLYLRNVEELTPALDLLCSAIRESDSETQSKHFSEQLLNLTLSFLNKQIKELFIHNEELDEHLQKGVNILTSYINELRTFMIKFPWKLTDAIDEYDANENVPKVKESHMWEKLSVEEVITNAVLNNKIPEAQTFFRINSHSAQRLEELIRIGLDLVFDSLKKNNIKEASELLKNMGFDVKDQLLKICFYTTDKNIRDFLVEILKEKNYFSEKEKTTIDFVHRVEKFYSGHFQENVQIQSFPRYWIKEEDFFKHKSVLDSFLKHDHKDKFNRQDHRIVLNWAQWWNRITQESILLPRISPEEYKSCSPEALWRYLTARHDWLNIVTWIGEFQTQSSDSSLQQHKWPPLTVDVIDHNTCCSNYMRNEILDKLARNGIFLRSELEDFELFLLRLNRIGGVMQDTLPVQNYRSKEGWDFHSHFILYCLERSLQYLLYVYLDCYKLSPPNCPFLERKELHEAHPWFEFLVQCRQVSSNLTDPKLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQNEENENCLKKVDPQLLKMALTPYPKLKTALFPQYTAPSVLPPDITLYHLLQSLLPFDPSRLFGWQSANTLAIGDASSHLPHFSSPDLVNKYAIVERLNFAYYLHHGRPSFAFGTFLVQELnullQSKTPKQLIQQVGNEAYVLGLSSFHVPSIGAACVCFLELLGLDSLKLRVDMKVANIILSYKCGNEDAQYSFIRESLAEKLSKLAAGEKATTEELLILLEEGIWNSIQQQETKRLSSESSSQWALVVQFCRLHNVKLSTSYLIECAKANDWLQFIIHSQLHNYHPEEVKSLLQYFSPVLQDHLQLAFENLPSVSNSRMDNDQVCNRSPQELQRNKEDTTDFFKILLQCSEEPNSWCWLLAEAVKQQAPILSVLASCLQDSSAIPCLCVWIITSVEDSVAAEAMGQIQGSTEGHTWDLEDLSVIWRTLLTRQKSKTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAKAKLLEFQKNLETLETAATKVHTVIPAPWLKEQVCFLLKLMLQQCRTQYELGKLLQLFVGVEHLFPDGPDVKKLCVLSQILKDTSIAINHVIITSYSLETFQHECRSILEKLETDGQFALARRVAELAELPVDNLVIEEITQEMQTLKHIDQWSLKQAKIDFWKKCHENFKKNSISSKAASSFFSAQALVACAYPTEEGQSSVEERHLLLTLAGHWLAQEDPVPVNQLEELEKQIWLCRITQHTLRGSQGETEPRFSQQISTSGELSFDSLASEFSFSKLAALNTSKYLELNGLPSKETCENRLDWKEQASLHFLIGRLLDDGCVHEASRVCRYFHFYNQDVVLVLHCRALASGEAGVDDLHPEIHALLQSAALPEGEEEPGIPLRKAQSTSSLDSQSFVMLPSSDEVVTNLKTLTGKCLHGKNYCRQVLCLYELAKELGCSYTDVAAQDGEAMLRTILASQQPDRCKRAQAFISTQGLEPDTVAELVAEEVTRELLTPSERAGQKQMFTPAEESQTFLPLTTLCADRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAARLLTDNHLAPNEEYGLVVRLLTGIGRYNEMTYIFDLLHKKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEDSLKDGHQLKQLLLKALTLMLDAAESYAKDSCVRQALHCHRLTKLITLQIHFLNTGQNTMLINLSRHRLMDCIMTLPRFYQASIVAEAYDFVPDWAEILYQRVILKGDFNYLEEFKQQRLLKSNIFEEISKKYKQHQPTDTVVKNLKKLLTYCEDVYLYYKLAYEHKFCDIVNVLLKDPQIGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_014733570.1
Organism	Equus caballus(horse)
Definition	Equus caballus spastic paraplegia 11 (autosomal recessive) (SPG11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014733570.1

ORF Insert Sequence:

ATGGCTGGTT GCCGCAATAT GAAGATCATT GAGGACCTTG AGGTGGACAG GGACGCAGTT 
CAGCAGCTTT CCCCGGGAGG CACGGGTCTG CGAGCGGGTC TGCTACACTG CGCATGCCCA 
GGATGCAGGC GGGGTCCGGC GCGGGTGACC GGAAGAAACC GCCGAGCCAC GTTGGCCGCA 
GAGCAGGCGG CCGGAAGTGC CGCTGCCGGC GGTAGCTGCA ACGCTGCAGC CATGGAGCGG 
GTTCTGCCTA TGTTGTTGGT GCCAGTCCCC GCCGAGACAA CGGGGCGGCT GGGGGCCCGG 
GCGCAGCTGC ACAGAGAGCA GGAGGTTCTG GGGAGCCTGA CGGCCTCGGG CTGCCTTCAA 
GTGCTCTCCT TGGCGCCCGG AGGCCGGGGC GGGGGCGGCT GCCGCCTGGA TGGCCCCTTT 
CGGCATTTTG TGTGGGAGGA TTCTCATAAC AGCAGCGCAC CAACAGACAA GCCCAGACTG 
CTCGCTCTTA CTGACAATTA TGAACTGCTC GTCTATGAAT TTAATTTGAA AGATGGAAGA 
TGTGATGCAA CCATTTTGTA TAGCTATAGT GAGGAGAGAT TGCAAAAGCT CGTTGAAGAT 
CTAAATATCA GTATTTCCTT ATTATCTTTG AGAATTCTGT CATTTCACAA TAACACATCA 
TTATTGTTCA TCAACAAGTG TATCATCCTG CAGGTTGTAT TTCTTGAAAG AGATACTGAG 
ATTAGAGTAC TCAACTGTTT TACATTACCT CTGCCTACAC AGGCAGTGGA CAGGATTATT 
GACACGCAGC TCTGCAGAGG AATTCTCTTT GTTTTGAGTA GTTTAGGCTG GATCTATATT 
TTTGACATTG TGGACGGTAC ACATGTAGCT CATGTGGATT TAGCACTTCA CCAAGAAGAT 
ATGTGTAATG AGCAACAACA GGAGCCAGCC AAGATTTCTT CTTTTACTTC ACTGAAAGTG 
TCTCAAGACC TAGATGTTGT AGTGATTGTC AGCTCCTCCA ACTCTGCAAT TGCTCTTAAC 
TTAAATTTGT ATTTCAGGCA ACACCCAGGA CACCTACTAT GCGAAAGAAC TCTAGAAGAC 
AGTCCTATTG AAGGCCCTAA GGAGATAGAT GAAGATGATC CTGCTAATTC TGACTACAAC 
ATGAAACTGA CCAAGTTTTC CTTCCAAGTT GATAGGTCTT GGAAAGCCCA GCTATCATCA 
TTGAATGAAA CAATAAAGAG CTCCAAGCTG GAGGTTTCCC ATTGTGCTCC ATGGTTCCAG 
GATATTTTAC ACGTGGAGCC TTCTGAATCT GGTAACCACG GTACCAGTGT GCCAGGCTGG 
GCCTTCATTC CACAGGACAT AATGGGCCGC CAGTATAATT TTCCACAGAA AGATCACACC 
AAGACCAATG ATCCAGGAAG ATCCTGGAAA GTAGTGCACA TCAATGAACA AGAGGAACCC 
ATCAAGCTTA AATGTGTGTC TGTGACAGGA TTCACTGCAC TGTTTACTTG GGAAGCAGAG 
AGGATGGGCT GCACCATTGT CCTTTGGGAT TTGGAGACCC AGGGCATGCA GTGTTGTCCT 
CTTGGCAAAA AATGTATTCC TGTAGACAGC AGTGGAGACC AGCAGCTGTG CCTTGTTTTG 
ACAGAAAGTG GACTTTCCCT GATTTTGTTT GGTTTGACTC AAGAGGAGTT TTTAAATAGA 
CTAATGATCC ATGGAAGTGC CAGCACTGTG GACTCTCTCT GCCATCTCAA TGGTTGGGGG 
AGGTGCTCAA TCCCCATACA TGCACTAGAG GCTGGCATAG AAAATCGTCA GCTGGACACA 
GTAGATTTCT TTTTGAAGAG CAAGGAAAAT CTTTTGAATC CATCCTCAAA ATCTTCTGTA 
GCTGATCAGC TTGACCACTT TCCATCCCAT TTGTATTTAA GAAATGTGGA AGAGCTGACA 
CCAGCATTGG ATTTACTTTG CTCAGCAATT AGAGAAAGTG ATTCTGAAAC CCAGAGCAAA 
CACTTCTCAG AACAGTTGCT TAATCTTACA CTGTCTTTCC TTAACAAACA AATAAAGGAG 
CTTTTTATTC ACAATGAAGA GCTAGATGAA CATCTACAGA AGGGAGTGAA CATTTTGACC 
AGCTACATTA ATGAACTTCG AACCTTCATG ATAAAGTTTC CTTGGAAGCT AACAGATGCC 
ATAGATGAAT ATGATGCAAA TGAAAATGTC CCCAAAGTAA AGGAGAGCCA TATGTGGGAG 
AAACTCAGCG TTGAGGAAGT TATCACCAAT GCCGTTTTAA ACAACAAAAT ACCAGAGGCA 
CAAACTTTCT TTAGGATTAA CAGTCATTCT GCTCAAAGAC TTGAGGAGCT GATTAGAATA 
GGCCTAGATT TGGTCTTTGA CAGTTTGAAA AAGAATAATA TAAAGGAAGC CTCCGAACTT 
TTGAAGAATA TGGGCTTTGA TGTAAAAGAC CAGCTGCTCA AGATATGCTT CTATACGACT 
GATAAAAATA TACGGGACTT TTTGGTTGAA ATTTTAAAAG AAAAAAATTA TTTTTCTGAA 
AAAGAAAAAA CAACTATAGA CTTTGTGCAT CGAGTTGAGA AGTTTTATTC AGGACATTTC 
CAAGAAAATG TGCAGATCCA GTCCTTTCCC AGATATTGGA TAAAGGAAGA AGATTTTTTC 
AAGCACAAAT CTGTTTTGGA CTCATTCCTG AAACATGACC ATAAAGATAA ATTTAACAGA 
CAGGATCATA GAATTGTGTT AAATTGGGCC CAGTGGTGGA ATCGAATAAC ACAAGAATCC 
ATCCTTCTCC CCAGGATAAG TCCAGAAGAA TACAAATCAT GTTCTCCTGA AGCCCTCTGG 
AGGTACCTCA CAGCTCGCCA TGATTGGTTA AACATTGTCA CGTGGATTGG AGAATTTCAG 
ACCCAGAGTA GTGATTCTTC ACTTCAGCAG CACAAGTGGC CCCCTCTGAC TGTTGATGTT 
ATTGACCACA ACACTTGCTG CAGCAACTAC ATGAGGAATG AAATTTTAGA TAAGTTGGCC 
AGGAATGGGA TTTTTCTTAG ATCTGAACTA GAAGACTTTG AACTCTTCCT CTTAAGACTG 
AACCGTATTG GGGGTGTGAT GCAAGATACC CTCCCTGTTC AAAACTACAG GTCCAAAGAA 
GGTTGGGACT TCCATTCTCA CTTCATTCTC TATTGTTTGG AACGCAGTCT GCAGTATCTT 
CTTTATGTCT ATCTAGACTG TTACAAACTT AGTCCTCCTA ATTGTCCTTT TTTGGAAAGA 
AAAGAATTAC ATGAAGCTCA CCCGTGGTTT GAATTTTTAG TTCAGTGTCG ACAAGTTTCC 
AGTAATTTAA CAGATCCCAA ATTAATCTTC CAGGCTAGCC TTGCAAATGC TCAGATTCTG 
ATTCCCAGCA ACCAGGCCAG TGTGAGCAGT ATGCTATTGG AAGGACATAC CCTCCTGGCC 
CTTGCTACCA CAATGTATGC TCCTGGGGGT GTCAGCCAGG TTGTTCAGAA TGAAGAAAAT 
GAGAACTGTT TGAAGAAAGT AGACCCCCAG CTACTGAAGA TGGCATTAAC TCCTTACCCC 
AAGCTTAAAA CTGCTCTCTT CCCACAGTAC ACTGCCCCTA GTGTCCTGCC ACCCGACATT 
ACACTCTACC ACCTTCTTCA GTCGTTATTG CCCTTTGATC CCAGCAGATT GTTTGGCTGG 
CAGTCTGCCA ACACACTAGC TATAGGAGAT GCATCGAGTC ACCTCCCCCA TTTCTCTAGC 
CCAGACCTGG TTAATAAGTA TGCTATCGTG GAACGTCTGA ATTTTGCTTA TTATTTACAT 
CATGGACGGC CATCATTTGC ATTTGGTACT TTTCTGGTCC AGGAATTANA CCAGAGCAAG 
ACTCCCAAGC AGCTGATCCA GCAAGTAGGC AATGAAGCCT ATGTTTTAGG CCTCTCCTCC 
TTCCATGTAC CTTCAATAGG AGCTGCCTGT GTTTGTTTCT TGGAATTGCT TGGCCTCGAC 
AGCCTCAAGC TCAGAGTTGA TATGAAAGTG GCCAATATAA TTTTGAGCTA CAAGTGTGGA 
AATGAAGATG CTCAGTACAG CTTTATCAGG GAGTCTCTAG CTGAAAAACT ATCTAAACTG 
GCTGCGGGTG AAAAGGCAAC CACAGAAGAA TTGCTTATTC TCTTAGAAGA GGGTATATGG 
AACAGCATTC AGCAGCAGGA AACAAAGAGA TTATCCAGTG AATCCAGCAG CCAATGGGCA 
TTAGTGGTAC AATTCTGCAG GCTCCACAAT GTGAAACTGA GCACGTCTTA CCTTATAGAG 
TGTGCCAAAG CAAATGACTG GTTACAGTTC ATTATTCATA GCCAGCTCCA TAATTACCAC 
CCAGAGGAGG TGAAATCTCT TCTCCAGTAT TTCAGCCCTG TCCTTCAAGA CCACTTACAG 
CTGGCTTTTG AGAACTTGCC CTCAGTGTCC AACTCCAGAA TGGACAATGA TCAAGTCTGC 
AACAGGTCCC CCCAGGAACT CCAGAGAAAC AAAGAAGATA CGACTGATTT CTTTAAAATT 
CTGCTGCAGT GCTCAGAGGA GCCAAACTCC TGGTGTTGGC TCCTAGCTGA AGCAGTGAAA 
CAACAGGCCC CTATCCTCAG TGTCCTGGCC TCGTGTCTCC AGGATTCCAG TGCTATTCCT 
TGTCTCTGTG TTTGGATCAT CACTTCTGTG GAGGACAGTG TTGCAGCTGA AGCAATGGGA 
CAAATTCAGG GTTCAACGGA GGGCCACACT TGGGACCTTG AGGACCTCTC TGTCATCTGG 
AGGACATTAT TAACGAGACA GAAGAGCAAA ACTCTCATCA GAGGTTTCCA ACTTTTCTTT 
AAGGATTCCC CGTTGCTATT GATGATGGAA ATGTATGAAC TGTGTATGTT CTTCAAGAAT 
TATAAGGAAG CCAAAGCAAA ACTATTGGAG TTCCAGAAGA ATCTTGAAAC TCTTGAAACA 
GCAGCCACAA AGGTCCACAC TGTCATCCCT GCCCCATGGC TGAAGGAGCA GGTGTGCTTT 
CTTTTGAAGC TTATGCTGCA GCAGTGTAGG ACCCAGTATG AGCTGGGGAA GCTTTTACAG 
CTGTTTGTTG GAGTAGAGCA TCTCTTCCCT GATGGTCCAG ATGTGAAGAA GCTGTGTGTC 
CTCAGCCAGA TTTTGAAAGA TACGTCCATA GCCATTAATC ATGTAATTAT TACCAGCTAC 
AGCCTTGAGA CTTTTCAGCA TGAATGTAGA TCTATCTTGG AAAAACTGGA GACAGATGGA 
CAGTTTGCTT TGGCCAGGAG AGTAGCAGAA TTAGCTGAGT TACCTGTGGA CAACTTGGTT 
ATTGAAGAGA TAACACAGGA AATGCAGACC TTAAAACACA TTGACCAGTG GTCTCTGAAA 
CAAGCAAAAA TTGACTTCTG GAAAAAATGC CATGAGAATT TTAAGAAAAA TTCCATTTCA 
AGCAAAGCAG CTTCTTCCTT TTTCTCAGCC CAGGCCCTTG TGGCATGTGC GTACCCGACT 
GAGGAGGGGC AGAGCAGCGT TGAGGAGCGC CATCTGCTGC TCACCCTGGC AGGGCACTGG 
CTTGCTCAGG AGGACCCGGT GCCTGTCAAC CAGCTGGAGG AGCTGGAGAA GCAGATCTGG 
CTGTGCCGCA TCACCCAGCA CACCCTCAGA GGAAGCCAGG GGGAAACAGA GCCCAGATTT 
TCTCAACAGA TCTCAACTAG TGGGGAACTT TCTTTTGATA GCTTAGCCAG TGAGTTTTCA 
TTCTCCAAGT TGGCTGCTCT GAACACATCA AAATACTTAG AACTTAATGG CCTTCCATCC 
AAAGAGACTT GTGAGAACAG ACTGGATTGG AAGGAGCAGG CGTCACTACA CTTTTTGATT 
GGGCGCCTCT TGGATGATGG CTGTGTGCAT GAAGCAAGTA GAGTGTGCCG GTATTTTCAT 
TTCTACAACC AAGATGTTGT CTTGGTATTG CACTGTAGAG CACTGGCTTC AGGTGAAGCT 
GGTGTGGATG ACCTGCATCC AGAGATCCAT GCTCTCCTAC AAAGTGCTGC GCTGCCTGAG 
GGAGAAGAAG AACCTGGCAT TCCTCTAAGG AAAGCCCAAA GCACTTCAAG TTTGGATAGT 
CAGTCGTTTG TGATGCTGCC CTCAAGTGAT GAAGTGGTGA CTAATCTGAA AACTCTGACT 
GGCAAATGCC TCCATGGGAA AAACTACTGC CGGCAGGTCC TCTGTCTGTA TGAACTTGCC 
AAGGAGTTGG GTTGTTCTTA CACAGATGTG GCTGCCCAGG ATGGTGAGGC CATGCTCCGG 
ACAATCTTGG CCTCTCAGCA GCCTGACCGG TGCAAGCGAG CCCAAGCCTT CATCAGCACC 
CAGGGCCTCG AGCCAGACAC GGTGGCTGAA CTTGTGGCCG AGGAGGTGAC CAGAGAGCTG 
CTGACCCCAT CAGAAAGAGC AGGACAGAAG CAGATGTTCA CCCCAGCAGA GGAAAGCCAG 
ACTTTTCTTC CACTGACCAC TTTGTGTGCA GACCGCACAT TGGTAGGCAT GAAGTTGTTG 
GATAAGATTT CCTCCGTTCC CCATGGGGAG TTGTCTTGCA CCACAGAGCT GCTGATCCTG 
GCCCATCACT GCTTCACGCT GACATGCCAC ATGGAGGGCA TCATCCGAGT CCTGCAGGCC 
GCCCGGCTGC TCACAGACAA CCACTTGGCC CCCAATGAAG AGTACGGGCT GGTGGTGCGT 
CTCCTCACTG GCATTGGAAG GTACAATGAG ATGACATACA TATTTGATTT GCTGCATAAA 
AAGCACTACT TTGAAGTGCT GATGAGGAAG AAATTAGATC CGAGTGGTAC CCTGAAGACA 
GCCCTACTCG ACTACATCAA ACGCTGCCGC CCTGGGGACA GTGAGAAGCA CAACATGATT 
GCCTTGTGCT TCAGCATGTG CCGTGAGATT GGGGAGAACC ATGAGGCAGC TGCCTGCATC 
CAGCTGAAAT TGATCGAGTC TCAGCCCTGG GAGGACAGCC TCAAAGATGG GCACCAACTG 
AAGCAGCTGC TGCTGAAGGC CCTGACTCTG ATGCTGGATG CAGCAGAGAG TTATGCCAAG 
GACTCTTGCG TGCGACAGGC CCTGCACTGT CATCGGCTCA CCAAGTTGAT AACACTGCAG 
ATTCACTTTC TGAACACTGG CCAGAACACA ATGCTCATCA ACCTGAGCCG CCACAGGCTG 
ATGGACTGTA TCATGACCCT ACCTCGGTTC TACCAGGCTT CTATTGTGGC GGAGGCCTAT 
GACTTTGTTC CTGATTGGGC TGAAATTCTA TACCAACGAG TGATTCTTAA AGGAGACTTT 
AATTACTTGG AAGAGTTTAA GCAGCAAAGG TTATTAAAAT CCAATATATT TGAAGAGATT 
TCAAAAAAAT ATAAACAACA TCAGCCTACC GACACAGTTG TGAAAAACCT GAAGAAGTTA 
CTCACGTATT GTGAAGATGT CTACTTGTAT TACAAACTAG CTTATGAACA CAAATTTTGT 
GATATTGTAA ATGTGCTTCT GAAGGACCCT CAGATAGGGT GCTGTTTAAA GGACATGCTA 
GCAGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_023618492.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023474260.1 Equus caballus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X1, mRNA.

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CloneID	OHb42160
Clone ID Related Accession (Same CDS sequence)	XM_023618492.1
Accession Version	XM_023618492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	spatacsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGG TTCTGCCTAT GTTGTTGGTG CCAGTCCCCG CCGAGACAAC GGGGCGGCTG 
GGGGCCCGGG CGCAGCTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTGAC GGCCTCGGGC 
TGCCTTCAAG TGCTCTCCTT GGCGCCCGGA GGCCGGGGCG GGGGCGGCTG CCGCCTGGAT 
GGCCCCTTTC GGCATTTTGT GTGGGAGGAT TCTCATAACA GCAGCGCACC AACAGACAAG 
CCCAGACTGC TCGCTCTTAC TGACAATTAT GAACTGCTCG TCTATGAATT TAATTTGAAA 
GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTATAGTG AGGAGAGATT GCAAAAGCTC 
GTTGAAGATC TAAATATCAG TATTTCCTTA TTATCTTTGA GAATTCTGTC ATTTCACAAT 
AACACATCAT TATTGTTCAT CAACAAGTGT ATCATCCTGC AGGTTGTATT TCTTGAAAGA 
GATACTGAGA TTAGAGTACT CAACTGTTTT ACATTACCTC TGCCTACACA GGCAGTGGAC 
AGGATTATTG ACACGCAGCT CTGCAGAGGA ATTCTCTTTG TTTTGAGTAG TTTAGGCTGG 
ATCTATATTT TTGACATTGT GGACGGTACA CATGTAGCTC ATGTGGATTT AGCACTTCAC 
CAAGAAGATA TGTGTAATGA GCAACAACAG GAGCCAGCCA AGATTTCTTC TTTTACTTCA 
CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTCCAA CTCTGCAATT 
GCTCTTAACT TAAATTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTATG CGAAAGAACT 
CTAGAAGACA GTCCTATTGA AGGCCCTAAG GAGATAGATG AAGATGATCC TGCTAATTCT 
GACTACAACA TGAAACTGAC CAAGTTTTCC TTCCAAGTTG ATAGGTCTTG GAAAGCCCAG 
CTATCATCAT TGAATGAAAC AATAAAGAGC TCCAAGCTGG AGGTTTCCCA TTGTGCTCCA 
TGGTTCCAGG ATATTTTACA CGTGGAGCCT TCTGAATCTG GTAACCACGG TACCAGTGTG 
CCAGGCTGGG CCTTCATTCC ACAGGACATA ATGGGCCGCC AGTATAATTT TCCACAGAAA 
GATCACACCA AGACCAATGA TCCAGGAAGA TCCTGGAAAG TAGTGCACAT CAATGAACAA 
GAGGAACCCA TCAAGCTTAA ATGTGTGTCT GTGACAGGAT TCACTGCACT GTTTACTTGG 
GAAGCAGAGA GGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGCATGCAG 
TGTTGTCCTC TTGGCAAAAA ATGTATTCCT GTAGACAGCA GTGGAGACCA GCAGCTGTGC 
CTTGTTTTGA CAGAAAGTGG ACTTTCCCTG ATTTTGTTTG GTTTGACTCA AGAGGAGTTT 
TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTCTG CCATCTCAAT 
GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCACTAGAGG CTGGCATAGA AAATCGTCAG 
CTGGACACAG TAGATTTCTT TTTGAAGAGC AAGGAAAATC TTTTGAATCC ATCCTCAAAA 
TCTTCTGTAG CTGATCAGCT TGACCACTTT CCATCCCATT TGTATTTAAG AAATGTGGAA 
GAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC 
CAGAGCAAAC ACTTCTCAGA ACAGTTGCTT AATCTTACAC TGTCTTTCCT TAACAAACAA 
ATAAAGGAGC TTTTTATTCA CAATGAAGAG CTAGATGAAC ATCTACAGAA GGGAGTGAAC 
ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAGTTTCC TTGGAAGCTA 
ACAGATGCCA TAGATGAATA TGATGCAAAT GAAAATGTCC CCAAAGTAAA GGAGAGCCAT 
ATGTGGGAGA AACTCAGCGT TGAGGAAGTT ATCACCAATG CCGTTTTAAA CAACAAAATA 
CCAGAGGCAC AAACTTTCTT TAGGATTAAC AGTCATTCTG CTCAAAGACT TGAGGAGCTG 
ATTAGAATAG GCCTAGATTT GGTCTTTGAC AGTTTGAAAA AGAATAATAT AAAGGAAGCC 
TCCGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGCTGCTCAA GATATGCTTC 
TATACGACTG ATAAAAATAT ACGGGACTTT TTGGTTGAAA TTTTAAAAGA AAAAAATTAT 
TTTTCTGAAA AAGAAAAAAC AACTATAGAC TTTGTGCATC GAGTTGAGAA GTTTTATTCA 
GGACATTTCC AAGAAAATGT GCAGATCCAG TCCTTTCCCA GATATTGGAT AAAGGAAGAA 
GATTTTTTCA AGCACAAATC TGTTTTGGAC TCATTCCTGA AACATGACCA TAAAGATAAA 
TTTAACAGAC AGGATCATAG AATTGTGTTA AATTGGGCCC AGTGGTGGAA TCGAATAACA 
CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA 
GCCCTCTGGA GGTACCTCAC AGCTCGCCAT GATTGGTTAA ACATTGTCAC GTGGATTGGA 
GAATTTCAGA CCCAGAGTAG TGATTCTTCA CTTCAGCAGC ACAAGTGGCC CCCTCTGACT 
GTTGATGTTA TTGACCACAA CACTTGCTGC AGCAACTACA TGAGGAATGA AATTTTAGAT 
AAGTTGGCCA GGAATGGGAT TTTTCTTAGA TCTGAACTAG AAGACTTTGA ACTCTTCCTC 
TTAAGACTGA ACCGTATTGG GGGTGTGATG CAAGATACCC TCCCTGTTCA AAACTACAGG 
TCCAAAGAAG GTTGGGACTT CCATTCTCAC TTCATTCTCT ATTGTTTGGA ACGCAGTCTG 
CAGTATCTTC TTTATGTCTA TCTAGACTGT TACAAACTTA GTCCTCCTAA TTGTCCTTTT 
TTGGAAAGAA AAGAATTACA TGAAGCTCAC CCGTGGTTTG AATTTTTAGT TCAGTGTCGA 
CAAGTTTCCA GTAATTTAAC AGATCCCAAA TTAATCTTCC AGGCTAGCCT TGCAAATGCT 
CAGATTCTGA TTCCCAGCAA CCAGGCCAGT GTGAGCAGTA TGCTATTGGA AGGACATACC 
CTCCTGGCCC TTGCTACCAC AATGTATGCT CCTGGGGGTG TCAGCCAGGT TGTTCAGAAT 
GAAGAAAATG AGAACTGTTT GAAGAAAGTA GACCCCCAGC TACTGAAGAT GGCATTAACT 
CCTTACCCCA AGCTTAAAAC TGCTCTCTTC CCACAGTACA CTGCCCCTAG TGTCCTGCCA 
CCCGACATTA CACTCTACCA CCTTCTTCAG TCGTTATTGC CCTTTGATCC CAGCAGATTG 
TTTGGCTGGC AGTCTGCCAA CACACTAGCT ATAGGAGATG CATCGAGTCA CCTCCCCCAT 
TTCTCTAGCC CAGACCTGGT TAATAAGTAT GCTATCGTGG AACGTCTGAA TTTTGCTTAT 
TATTTACATC ATGGACGGCC ATCATTTGCA TTTGGTACTT TTCTGGTCCA GGAATTAACC 
AAGAGCAAGA CTCCCAAGCA GCTGATCCAG CAAGTAGGCA ATGAAGCCTA TGTTTTAGGC 
CTCTCCTCCT TCCATGTACC TTCAATAGGA GCTGCCTGTG TTTGTTTCTT GGAATTGCTT 
GGCCTCGACA GCCTCAAGCT CAGAGTTGAT ATGAAAGTGG CCAATATAAT TTTGAGCTAC 
AAGTGTGGAA ATGAAGATGC TCAGTACAGC TTTATCAGGG AGTCTCTAGC TGAAAAACTA 
TCTAAACTGG CTGCGGGTGA AAAGGCAACC ACAGAAGAAT TGCTTATTCT CTTAGAAGAG 
GGTATATGGA ACAGCATTCA GCAGCAGGAA ACAAAGAGAT TATCCAGTGA ATCCAGCAGC 
CAATGGGCAT TAGTGGTACA ATTCTGCAGG CTCCACAATG TGAAACTGAG CACGTCTTAC 
CTTATAGAGT GTGCCAAAGC AAATGACTGG TTACAGTTCA TTATTCATAG CCAGCTCCAT 
AATTACCACC CAGAGGAGGT GAAATCTCTT CTCCAGTATT TCAGCCCTGT CCTTCAAGAC 
CACTTACAGC TGGCTTTTGA GAACTTGCCC TCAGTGTCCA ACTCCAGAAT GGACAATGAT 
CAAGTCTGCA ACAGGTCCCC CCAGGAACTC CAGAGAAACA AAGAAGATAC GACTGATTTC 
TTTAAAATTC TGCTGCAGTG CTCAGAGGAG CCAAACTCCT GGTGTTGGCT CCTAGCTGAA 
GCAGTGAAAC AACAGGCCCC TATCCTCAGT GTCCTGGCCT CGTGTCTCCA GGATTCCAGT 
GCTATTCCTT GTCTCTGTGT TTGGATCATC ACTTCTGTGG AGGACAGTGT TGCAGCTGAA 
GCAATGGGAC AAATTCAGGG TTCAACGGAG GGCCACACTT GGGACCTTGA GGACCTCTCT 
GTCATCTGGA GGACATTATT AACGAGACAG AAGAGCAAAA CTCTCATCAG AGGTTTCCAA 
CTTTTCTTTA AGGATTCCCC GTTGCTATTG ATGATGGAAA TGTATGAACT GTGTATGTTC 
TTCAAGAATT ATAAGGAAGC CAAAGCAAAA CTATTGGAGT TCCAGAAGAA TCTTGAAACT 
CTTGAAACAG CAGCCACAAA GGTCCACACT GTCATCCCTG CCCCATGGCT GAAGGAGCAG 
GTGTGCTTTC TTTTGAAGCT TATGCTGCAG CAGTGTAGGA CCCAGTATGA GCTGGGGAAG 
CTTTTACAGC TGTTTGTTGG AGTAGAGCAT CTCTTCCCTG ATGGTCCAGA TGTGAAGAAG 
CTGTGTGTCC TCAGCCAGAT TTTGAAAGAT ACGTCCATAG CCATTAATCA TGTAATTATT 
ACCAGCTACA GCCTTGAGAC TTTTCAGCAT GAATGTAGAT CTATCTTGGA AAAACTGGAG 
ACAGATGGAC AGTTTGCTTT GGCCAGGAGA GTAGCAGAAT TAGCTGAGTT ACCTGTGGAC 
AACTTGGTTA TTGAAGAGAT AACACAGGAA ATGCAGACCT TAAAACACAT TGACCAGTGG 
TCTCTGAAAC AAGCAAAAAT TGACTTCTGG AAAAAATGCC ATGAGAATTT TAAGAAAAAT 
TCCATTTCAA GCAAAGCAGC TTCTTCCTTT TTCTCAGCCC AGGCCCTTGT GGCATGTGCG 
TACCCGACTG AGGAGGGGCA GAGCAGCGTT GAGGAGCGCC ATCTGCTGCT CACCCTGGCA 
GGGCACTGGC TTGCTCAGGA GGACCCGGTG CCTGTCAACC AGCTGGAGGA GCTGGAGAAG 
CAGATCTGGC TGTGCCGCAT CACCCAGCAC ACCCTCAGAG GAAGCCAGGG GGAAACAGAG 
CCCAGATTTT CTCAACAGAT CTCAACTAGT GGGGAACTTT CTTTTGATAG CTTAGCCAGT 
GAGTTTTCAT TCTCCAAGTT GGCTGCTCTG AACACATCAA AATACTTAGA ACTTAATGGC 
CTTCCATCCA AAGAGACTTG TGAGAACAGA CTGGATTGGA AGGAGCAGGC GTCACTACAC 
TTTTTGATTG GGCGCCTCTT GGATGATGGC TGTGTGCATG AAGCAAGTAG AGTGTGCCGG 
TATTTTCATT TCTACAACCA AGATGTTGTC TTGGTATTGC ACTGTAGAGC ACTGGCTTCA 
GGTGAAGCTG GTGTGGATGA CCTGCATCCA GAGATCCATG CTCTCCTACA AAGTGCTGCG 
CTGCCTGAGG GAGAAGAAGA ACCTGGCATT CCTCTAAGGA AAGCCCAAAG CAATGTGGCT 
GCCCAGGATG GTGAGGCCAT GCTCCGGACA ATCTTGGCCT CTCAGCAGCC TGACCGGTGC 
AAGCGAGCCC AAGCCTTCAT CAGCACCCAG GGCCTCGAGC CAGACACGGT GGCTGAACTT 
GTGGCCGAGG AGGTGACCAG AGAGCTGCTG ACCCCATCAG AAAGAGCAGG ACAGAAGCAG 
ATGTTCACCC CAGCAGAGGA AAGCCAGACT TTTCTTCCAC TGACCACTTT GTGTGCAGAC 
CGCACATTGG TAGGCATGAA GTTGTTGGAT AAGATTTCCT CCGTTCCCCA TGGGGAGTTG 
TCTTGCACCA CAGAGCTGCT GATCCTGGCC CATCACTGCT TCACGCTGAC ATGCCACATG 
GAGGGCATCA TCCGAGTCCT GCAGGCCGCC CGGCTGCTCA CAGACAACCA CTTGGCCCCC 
AATGAAGAGT ACGGGCTGGT GGTGCGTCTC CTCACTGGCA TTGGAAGGTA CAATGAGATG 
ACATACATAT TTGATTTGCT GCATAAAAAG CACTACTTTG AAGTGCTGAT GAGGAAGAAA 
TTAGATCCGA GTGGTACCCT GAAGACAGCC CTACTCGACT ACATCAAACG CTGCCGCCCT 
GGGGACAGTG AGAAGCACAA CATGATTGCC TTGTGCTTCA GCATGTGCCG TGAGATTGGG 
GAGAACCATG AGGCAGCTGC CTGCATCCAG CTGAAATTGA TCGAGTCTCA GCCCTGGGAG 
GACAGCCTCA AAGATGGGCA CCAACTGAAG CAGCTGCTGC TGAAGGCCCT GACTCTGATG 
CTGGATGCAG CAGAGAGTTA TGCCAAGGAC TCTTGCGTGC GACAGGCCCT GCACTGTCAT 
CGGCTCACCA AGTTGATAAC ACTGCAGATT CACTTTCTGA ACACTGGCCA GAACACAATG 
CTCATCAACC TGAGCCGCCA CAGGCTGATG GACTGTATCA TGACCCTACC TCGGTTCTAC 
CAGGCTTCTA TTGTGGCGGA GGCCTATGAC TTTGTTCCTG ATTGGGCTGA AATTCTATAC 
CAACGAGTGA TTCTTAAAGG AGACTTTAAT TACTTGGAAG AGTTTAAGCA GCAAAGGTTA 
TTAAAATCCA ATATATTTGA AGAGATTTCA AAAAAATATA AACAACATCA GCCTACCGAC 
ACAGTTGTGA AAAACCTGAA GAAGTTACTC ACGTATTGTG AAGATGTCTA CTTGTATTAC 
AAACTAGCTT ATGAACACAA ATTTTGTGAT ATTGTAAATG TGCTTCTGAA GGACCCTCAG 
ATAGGGTGCT GTTTAAAGGA CATGCTAGCA GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023474260.1
CDS	96..7211
Translation	MERVLPMLLVPVPAETTGRLGARAQLHREQEVLGSLTASGCLQVLSLAPGGRGGGGCRLDGPFRHFVWEDSHNSSAPTDKPRLLALTDNYELLVYEFNLKDGRCDATILYSYSEERLQKLVEDLNISISLLSLRILSFHNNTSLLFINKCIILQVVFLERDTEIRVLNCFTLPLPTQAVDRIIDTQLCRGILFVLSSLGWIYIFDIVDGTHVAHVDLALHQEDMCNEQQQEPAKISSFTSLKVSQDLDVVVIVSSSNSAIALNLNLYFRQHPGHLLCERTLEDSPIEGPKEIDEDDPANSDYNMKLTKFSFQVDRSWKAQLSSLNETIKSSKLEVSHCAPWFQDILHVEPSESGNHGTSVPGWAFIPQDIMGRQYNFPQKDHTKTNDPGRSWKVVHINEQEEPIKLKCVSVTGFTALFTWEAERMGCTIVLWDLETQGMQCCPLGKKCIPVDSSGDQQLCLVLTESGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLNPSSKSSVADQLDHFPSHLYLRNVEELTPALDLLCSAIRESDSETQSKHFSEQLLNLTLSFLNKQIKELFIHNEELDEHLQKGVNILTSYINELRTFMIKFPWKLTDAIDEYDANENVPKVKESHMWEKLSVEEVITNAVLNNKIPEAQTFFRINSHSAQRLEELIRIGLDLVFDSLKKNNIKEASELLKNMGFDVKDQLLKICFYTTDKNIRDFLVEILKEKNYFSEKEKTTIDFVHRVEKFYSGHFQENVQIQSFPRYWIKEEDFFKHKSVLDSFLKHDHKDKFNRQDHRIVLNWAQWWNRITQESILLPRISPEEYKSCSPEALWRYLTARHDWLNIVTWIGEFQTQSSDSSLQQHKWPPLTVDVIDHNTCCSNYMRNEILDKLARNGIFLRSELEDFELFLLRLNRIGGVMQDTLPVQNYRSKEGWDFHSHFILYCLERSLQYLLYVYLDCYKLSPPNCPFLERKELHEAHPWFEFLVQCRQVSSNLTDPKLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQNEENENCLKKVDPQLLKMALTPYPKLKTALFPQYTAPSVLPPDITLYHLLQSLLPFDPSRLFGWQSANTLAIGDASSHLPHFSSPDLVNKYAIVERLNFAYYLHHGRPSFAFGTFLVQELTKSKTPKQLIQQVGNEAYVLGLSSFHVPSIGAACVCFLELLGLDSLKLRVDMKVANIILSYKCGNEDAQYSFIRESLAEKLSKLAAGEKATTEELLILLEEGIWNSIQQQETKRLSSESSSQWALVVQFCRLHNVKLSTSYLIECAKANDWLQFIIHSQLHNYHPEEVKSLLQYFSPVLQDHLQLAFENLPSVSNSRMDNDQVCNRSPQELQRNKEDTTDFFKILLQCSEEPNSWCWLLAEAVKQQAPILSVLASCLQDSSAIPCLCVWIITSVEDSVAAEAMGQIQGSTEGHTWDLEDLSVIWRTLLTRQKSKTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAKAKLLEFQKNLETLETAATKVHTVIPAPWLKEQVCFLLKLMLQQCRTQYELGKLLQLFVGVEHLFPDGPDVKKLCVLSQILKDTSIAINHVIITSYSLETFQHECRSILEKLETDGQFALARRVAELAELPVDNLVIEEITQEMQTLKHIDQWSLKQAKIDFWKKCHENFKKNSISSKAASSFFSAQALVACAYPTEEGQSSVEERHLLLTLAGHWLAQEDPVPVNQLEELEKQIWLCRITQHTLRGSQGETEPRFSQQISTSGELSFDSLASEFSFSKLAALNTSKYLELNGLPSKETCENRLDWKEQASLHFLIGRLLDDGCVHEASRVCRYFHFYNQDVVLVLHCRALASGEAGVDDLHPEIHALLQSAALPEGEEEPGIPLRKAQSNVAAQDGEAMLRTILASQQPDRCKRAQAFISTQGLEPDTVAELVAEEVTRELLTPSERAGQKQMFTPAEESQTFLPLTTLCADRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAARLLTDNHLAPNEEYGLVVRLLTGIGRYNEMTYIFDLLHKKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEDSLKDGHQLKQLLLKALTLMLDAAESYAKDSCVRQALHCHRLTKLITLQIHFLNTGQNTMLINLSRHRLMDCIMTLPRFYQASIVAEAYDFVPDWAEILYQRVILKGDFNYLEEFKQQRLLKSNIFEEISKKYKQHQPTDTVVKNLKKLLTYCEDVYLYYKLAYEHKFCDIVNVLLKDPQIGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_023618492.1
Organism	Equus caballus(horse)
Definition	Equus caballus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023618492.1

ORF Insert Sequence:

ATGGAGCGGG TTCTGCCTAT GTTGTTGGTG CCAGTCCCCG CCGAGACAAC GGGGCGGCTG 
GGGGCCCGGG CGCAGCTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTGAC GGCCTCGGGC 
TGCCTTCAAG TGCTCTCCTT GGCGCCCGGA GGCCGGGGCG GGGGCGGCTG CCGCCTGGAT 
GGCCCCTTTC GGCATTTTGT GTGGGAGGAT TCTCATAACA GCAGCGCACC AACAGACAAG 
CCCAGACTGC TCGCTCTTAC TGACAATTAT GAACTGCTCG TCTATGAATT TAATTTGAAA 
GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTATAGTG AGGAGAGATT GCAAAAGCTC 
GTTGAAGATC TAAATATCAG TATTTCCTTA TTATCTTTGA GAATTCTGTC ATTTCACAAT 
AACACATCAT TATTGTTCAT CAACAAGTGT ATCATCCTGC AGGTTGTATT TCTTGAAAGA 
GATACTGAGA TTAGAGTACT CAACTGTTTT ACATTACCTC TGCCTACACA GGCAGTGGAC 
AGGATTATTG ACACGCAGCT CTGCAGAGGA ATTCTCTTTG TTTTGAGTAG TTTAGGCTGG 
ATCTATATTT TTGACATTGT GGACGGTACA CATGTAGCTC ATGTGGATTT AGCACTTCAC 
CAAGAAGATA TGTGTAATGA GCAACAACAG GAGCCAGCCA AGATTTCTTC TTTTACTTCA 
CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTCCAA CTCTGCAATT 
GCTCTTAACT TAAATTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTATG CGAAAGAACT 
CTAGAAGACA GTCCTATTGA AGGCCCTAAG GAGATAGATG AAGATGATCC TGCTAATTCT 
GACTACAACA TGAAACTGAC CAAGTTTTCC TTCCAAGTTG ATAGGTCTTG GAAAGCCCAG 
CTATCATCAT TGAATGAAAC AATAAAGAGC TCCAAGCTGG AGGTTTCCCA TTGTGCTCCA 
TGGTTCCAGG ATATTTTACA CGTGGAGCCT TCTGAATCTG GTAACCACGG TACCAGTGTG 
CCAGGCTGGG CCTTCATTCC ACAGGACATA ATGGGCCGCC AGTATAATTT TCCACAGAAA 
GATCACACCA AGACCAATGA TCCAGGAAGA TCCTGGAAAG TAGTGCACAT CAATGAACAA 
GAGGAACCCA TCAAGCTTAA ATGTGTGTCT GTGACAGGAT TCACTGCACT GTTTACTTGG 
GAAGCAGAGA GGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGCATGCAG 
TGTTGTCCTC TTGGCAAAAA ATGTATTCCT GTAGACAGCA GTGGAGACCA GCAGCTGTGC 
CTTGTTTTGA CAGAAAGTGG ACTTTCCCTG ATTTTGTTTG GTTTGACTCA AGAGGAGTTT 
TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTCTG CCATCTCAAT 
GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCACTAGAGG CTGGCATAGA AAATCGTCAG 
CTGGACACAG TAGATTTCTT TTTGAAGAGC AAGGAAAATC TTTTGAATCC ATCCTCAAAA 
TCTTCTGTAG CTGATCAGCT TGACCACTTT CCATCCCATT TGTATTTAAG AAATGTGGAA 
GAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC 
CAGAGCAAAC ACTTCTCAGA ACAGTTGCTT AATCTTACAC TGTCTTTCCT TAACAAACAA 
ATAAAGGAGC TTTTTATTCA CAATGAAGAG CTAGATGAAC ATCTACAGAA GGGAGTGAAC 
ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAGTTTCC TTGGAAGCTA 
ACAGATGCCA TAGATGAATA TGATGCAAAT GAAAATGTCC CCAAAGTAAA GGAGAGCCAT 
ATGTGGGAGA AACTCAGCGT TGAGGAAGTT ATCACCAATG CCGTTTTAAA CAACAAAATA 
CCAGAGGCAC AAACTTTCTT TAGGATTAAC AGTCATTCTG CTCAAAGACT TGAGGAGCTG 
ATTAGAATAG GCCTAGATTT GGTCTTTGAC AGTTTGAAAA AGAATAATAT AAAGGAAGCC 
TCCGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGCTGCTCAA GATATGCTTC 
TATACGACTG ATAAAAATAT ACGGGACTTT TTGGTTGAAA TTTTAAAAGA AAAAAATTAT 
TTTTCTGAAA AAGAAAAAAC AACTATAGAC TTTGTGCATC GAGTTGAGAA GTTTTATTCA 
GGACATTTCC AAGAAAATGT GCAGATCCAG TCCTTTCCCA GATATTGGAT AAAGGAAGAA 
GATTTTTTCA AGCACAAATC TGTTTTGGAC TCATTCCTGA AACATGACCA TAAAGATAAA 
TTTAACAGAC AGGATCATAG AATTGTGTTA AATTGGGCCC AGTGGTGGAA TCGAATAACA 
CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA 
GCCCTCTGGA GGTACCTCAC AGCTCGCCAT GATTGGTTAA ACATTGTCAC GTGGATTGGA 
GAATTTCAGA CCCAGAGTAG TGATTCTTCA CTTCAGCAGC ACAAGTGGCC CCCTCTGACT 
GTTGATGTTA TTGACCACAA CACTTGCTGC AGCAACTACA TGAGGAATGA AATTTTAGAT 
AAGTTGGCCA GGAATGGGAT TTTTCTTAGA TCTGAACTAG AAGACTTTGA ACTCTTCCTC 
TTAAGACTGA ACCGTATTGG GGGTGTGATG CAAGATACCC TCCCTGTTCA AAACTACAGG 
TCCAAAGAAG GTTGGGACTT CCATTCTCAC TTCATTCTCT ATTGTTTGGA ACGCAGTCTG 
CAGTATCTTC TTTATGTCTA TCTAGACTGT TACAAACTTA GTCCTCCTAA TTGTCCTTTT 
TTGGAAAGAA AAGAATTACA TGAAGCTCAC CCGTGGTTTG AATTTTTAGT TCAGTGTCGA 
CAAGTTTCCA GTAATTTAAC AGATCCCAAA TTAATCTTCC AGGCTAGCCT TGCAAATGCT 
CAGATTCTGA TTCCCAGCAA CCAGGCCAGT GTGAGCAGTA TGCTATTGGA AGGACATACC 
CTCCTGGCCC TTGCTACCAC AATGTATGCT CCTGGGGGTG TCAGCCAGGT TGTTCAGAAT 
GAAGAAAATG AGAACTGTTT GAAGAAAGTA GACCCCCAGC TACTGAAGAT GGCATTAACT 
CCTTACCCCA AGCTTAAAAC TGCTCTCTTC CCACAGTACA CTGCCCCTAG TGTCCTGCCA 
CCCGACATTA CACTCTACCA CCTTCTTCAG TCGTTATTGC CCTTTGATCC CAGCAGATTG 
TTTGGCTGGC AGTCTGCCAA CACACTAGCT ATAGGAGATG CATCGAGTCA CCTCCCCCAT 
TTCTCTAGCC CAGACCTGGT TAATAAGTAT GCTATCGTGG AACGTCTGAA TTTTGCTTAT 
TATTTACATC ATGGACGGCC ATCATTTGCA TTTGGTACTT TTCTGGTCCA GGAATTAACC 
AAGAGCAAGA CTCCCAAGCA GCTGATCCAG CAAGTAGGCA ATGAAGCCTA TGTTTTAGGC 
CTCTCCTCCT TCCATGTACC TTCAATAGGA GCTGCCTGTG TTTGTTTCTT GGAATTGCTT 
GGCCTCGACA GCCTCAAGCT CAGAGTTGAT ATGAAAGTGG CCAATATAAT TTTGAGCTAC 
AAGTGTGGAA ATGAAGATGC TCAGTACAGC TTTATCAGGG AGTCTCTAGC TGAAAAACTA 
TCTAAACTGG CTGCGGGTGA AAAGGCAACC ACAGAAGAAT TGCTTATTCT CTTAGAAGAG 
GGTATATGGA ACAGCATTCA GCAGCAGGAA ACAAAGAGAT TATCCAGTGA ATCCAGCAGC 
CAATGGGCAT TAGTGGTACA ATTCTGCAGG CTCCACAATG TGAAACTGAG CACGTCTTAC 
CTTATAGAGT GTGCCAAAGC AAATGACTGG TTACAGTTCA TTATTCATAG CCAGCTCCAT 
AATTACCACC CAGAGGAGGT GAAATCTCTT CTCCAGTATT TCAGCCCTGT CCTTCAAGAC 
CACTTACAGC TGGCTTTTGA GAACTTGCCC TCAGTGTCCA ACTCCAGAAT GGACAATGAT 
CAAGTCTGCA ACAGGTCCCC CCAGGAACTC CAGAGAAACA AAGAAGATAC GACTGATTTC 
TTTAAAATTC TGCTGCAGTG CTCAGAGGAG CCAAACTCCT GGTGTTGGCT CCTAGCTGAA 
GCAGTGAAAC AACAGGCCCC TATCCTCAGT GTCCTGGCCT CGTGTCTCCA GGATTCCAGT 
GCTATTCCTT GTCTCTGTGT TTGGATCATC ACTTCTGTGG AGGACAGTGT TGCAGCTGAA 
GCAATGGGAC AAATTCAGGG TTCAACGGAG GGCCACACTT GGGACCTTGA GGACCTCTCT 
GTCATCTGGA GGACATTATT AACGAGACAG AAGAGCAAAA CTCTCATCAG AGGTTTCCAA 
CTTTTCTTTA AGGATTCCCC GTTGCTATTG ATGATGGAAA TGTATGAACT GTGTATGTTC 
TTCAAGAATT ATAAGGAAGC CAAAGCAAAA CTATTGGAGT TCCAGAAGAA TCTTGAAACT 
CTTGAAACAG CAGCCACAAA GGTCCACACT GTCATCCCTG CCCCATGGCT GAAGGAGCAG 
GTGTGCTTTC TTTTGAAGCT TATGCTGCAG CAGTGTAGGA CCCAGTATGA GCTGGGGAAG 
CTTTTACAGC TGTTTGTTGG AGTAGAGCAT CTCTTCCCTG ATGGTCCAGA TGTGAAGAAG 
CTGTGTGTCC TCAGCCAGAT TTTGAAAGAT ACGTCCATAG CCATTAATCA TGTAATTATT 
ACCAGCTACA GCCTTGAGAC TTTTCAGCAT GAATGTAGAT CTATCTTGGA AAAACTGGAG 
ACAGATGGAC AGTTTGCTTT GGCCAGGAGA GTAGCAGAAT TAGCTGAGTT ACCTGTGGAC 
AACTTGGTTA TTGAAGAGAT AACACAGGAA ATGCAGACCT TAAAACACAT TGACCAGTGG 
TCTCTGAAAC AAGCAAAAAT TGACTTCTGG AAAAAATGCC ATGAGAATTT TAAGAAAAAT 
TCCATTTCAA GCAAAGCAGC TTCTTCCTTT TTCTCAGCCC AGGCCCTTGT GGCATGTGCG 
TACCCGACTG AGGAGGGGCA GAGCAGCGTT GAGGAGCGCC ATCTGCTGCT CACCCTGGCA 
GGGCACTGGC TTGCTCAGGA GGACCCGGTG CCTGTCAACC AGCTGGAGGA GCTGGAGAAG 
CAGATCTGGC TGTGCCGCAT CACCCAGCAC ACCCTCAGAG GAAGCCAGGG GGAAACAGAG 
CCCAGATTTT CTCAACAGAT CTCAACTAGT GGGGAACTTT CTTTTGATAG CTTAGCCAGT 
GAGTTTTCAT TCTCCAAGTT GGCTGCTCTG AACACATCAA AATACTTAGA ACTTAATGGC 
CTTCCATCCA AAGAGACTTG TGAGAACAGA CTGGATTGGA AGGAGCAGGC GTCACTACAC 
TTTTTGATTG GGCGCCTCTT GGATGATGGC TGTGTGCATG AAGCAAGTAG AGTGTGCCGG 
TATTTTCATT TCTACAACCA AGATGTTGTC TTGGTATTGC ACTGTAGAGC ACTGGCTTCA 
GGTGAAGCTG GTGTGGATGA CCTGCATCCA GAGATCCATG CTCTCCTACA AAGTGCTGCG 
CTGCCTGAGG GAGAAGAAGA ACCTGGCATT CCTCTAAGGA AAGCCCAAAG CAATGTGGCT 
GCCCAGGATG GTGAGGCCAT GCTCCGGACA ATCTTGGCCT CTCAGCAGCC TGACCGGTGC 
AAGCGAGCCC AAGCCTTCAT CAGCACCCAG GGCCTCGAGC CAGACACGGT GGCTGAACTT 
GTGGCCGAGG AGGTGACCAG AGAGCTGCTG ACCCCATCAG AAAGAGCAGG ACAGAAGCAG 
ATGTTCACCC CAGCAGAGGA AAGCCAGACT TTTCTTCCAC TGACCACTTT GTGTGCAGAC 
CGCACATTGG TAGGCATGAA GTTGTTGGAT AAGATTTCCT CCGTTCCCCA TGGGGAGTTG 
TCTTGCACCA CAGAGCTGCT GATCCTGGCC CATCACTGCT TCACGCTGAC ATGCCACATG 
GAGGGCATCA TCCGAGTCCT GCAGGCCGCC CGGCTGCTCA CAGACAACCA CTTGGCCCCC 
AATGAAGAGT ACGGGCTGGT GGTGCGTCTC CTCACTGGCA TTGGAAGGTA CAATGAGATG 
ACATACATAT TTGATTTGCT GCATAAAAAG CACTACTTTG AAGTGCTGAT GAGGAAGAAA 
TTAGATCCGA GTGGTACCCT GAAGACAGCC CTACTCGACT ACATCAAACG CTGCCGCCCT 
GGGGACAGTG AGAAGCACAA CATGATTGCC TTGTGCTTCA GCATGTGCCG TGAGATTGGG 
GAGAACCATG AGGCAGCTGC CTGCATCCAG CTGAAATTGA TCGAGTCTCA GCCCTGGGAG 
GACAGCCTCA AAGATGGGCA CCAACTGAAG CAGCTGCTGC TGAAGGCCCT GACTCTGATG 
CTGGATGCAG CAGAGAGTTA TGCCAAGGAC TCTTGCGTGC GACAGGCCCT GCACTGTCAT 
CGGCTCACCA AGTTGATAAC ACTGCAGATT CACTTTCTGA ACACTGGCCA GAACACAATG 
CTCATCAACC TGAGCCGCCA CAGGCTGATG GACTGTATCA TGACCCTACC TCGGTTCTAC 
CAGGCTTCTA TTGTGGCGGA GGCCTATGAC TTTGTTCCTG ATTGGGCTGA AATTCTATAC 
CAACGAGTGA TTCTTAAAGG AGACTTTAAT TACTTGGAAG AGTTTAAGCA GCAAAGGTTA 
TTAAAATCCA ATATATTTGA AGAGATTTCA AAAAAATATA AACAACATCA GCCTACCGAC 
ACAGTTGTGA AAAACCTGAA GAAGTTACTC ACGTATTGTG AAGATGTCTA CTTGTATTAC 
AAACTAGCTT ATGAACACAA ATTTTGTGAT ATTGTAAATG TGCTTCTGAA GGACCCTCAG 
ATAGGGTGCT GTTTAAAGGA CATGCTAGCA GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_023618503.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023474271.1 Equus caballus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X3, mRNA.

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CloneID	OHb42161
Clone ID Related Accession (Same CDS sequence)	XM_023618503.1 , XM_023618497.1
Accession Version	XM_023618503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTAATG AGCAACAACA GGAGCCAGCC AAGATTTCTT CTTTTACTTC ACTGAAAGTG 
TCTCAAGACC TAGATGTTGT AGTGATTGTC AGCTCCTCCA ACTCTGCAAT TGCTCTTAAC 
TTAAATTTGT ATTTCAGGCA ACACCCAGGA CACCTACTAT GCGAAAGAAC TCTAGAAGAC 
AGTCCTATTG AAGGCCCTAA GGAGATAGAT GAAGATGATC CTGCTAATTC TGACTACAAC 
ATGAAACTGA CCAAGTTTTC CTTCCAAGTT GATAGGTCTT GGAAAGCCCA GCTATCATCA 
TTGAATGAAA CAATAAAGAG CTCCAAGCTG GAGGTTTCCC ATTGTGCTCC ATGGTTCCAG 
GATATTTTAC ACGTGGAGCC TTCTGAATCT GGTAACCACG GTACCAGTGT GCCAGGCTGG 
GCCTTCATTC CACAGGACAT AATGGGCCGC CAGTATAATT TTCCACAGAA AGATCACACC 
AAGACCAATG ATCCAGGAAG ATCCTGGAAA GTAGTGCACA TCAATGAACA AGAGGAACCC 
ATCAAGCTTA AATGTGTGTC TGTGACAGGA TTCACTGCAC TGTTTACTTG GGAAGCAGAG 
AGGATGGGCT GCACCATTGT CCTTTGGGAT TTGGAGACCC AGGGCATGCA GTGTTGTCCT 
CTTGGCAAAA AATGTATTCC TGTAGACAGC AGTGGAGACC AGCAGCTGTG CCTTGTTTTG 
ACAGAAAGTG GACTTTCCCT GATTTTGTTT GGTTTGACTC AAGAGGAGTT TTTAAATAGA 
CTAATGATCC ATGGAAGTGC CAGCACTGTG GACTCTCTCT GCCATCTCAA TGGTTGGGGG 
AGGTGCTCAA TCCCCATACA TGCACTAGAG GCTGGCATAG AAAATCGTCA GCTGGACACA 
GTAGATTTCT TTTTGAAGAG CAAGGAAAAT CTTTTGAATC CATCCTCAAA ATCTTCTGTA 
GCTGATCAGC TTGACCACTT TCCATCCCAT TTGTATTTAA GAAATGTGGA AGAGCTGACA 
CCAGCATTGG ATTTACTTTG CTCAGCAATT AGAGAAAGTG ATTCTGAAAC CCAGAGCAAA 
CACTTCTCAG AACAGTTGCT TAATCTTACA CTGTCTTTCC TTAACAAACA AATAAAGGAG 
CTTTTTATTC ACAATGAAGA GCTAGATGAA CATCTACAGA AGGGAGTGAA CATTTTGACC 
AGCTACATTA ATGAACTTCG AACCTTCATG ATAAAGTTTC CTTGGAAGCT AACAGATGCC 
ATAGATGAAT ATGATGCAAA TGAAAATGTC CCCAAAGTAA AGGAGAGCCA TATGTGGGAG 
AAACTCAGCG TTGAGGAAGT TATCACCAAT GCCGTTTTAA ACAACAAAAT ACCAGAGGCA 
CAAACTTTCT TTAGGATTAA CAGTCATTCT GCTCAAAGAC TTGAGGAGCT GATTAGAATA 
GGCCTAGATT TGGTCTTTGA CAGTTTGAAA AAGAATAATA TAAAGGAAGC CTCCGAACTT 
TTGAAGAATA TGGGCTTTGA TGTAAAAGAC CAGCTGCTCA AGATATGCTT CTATACGACT 
GATAAAAATA TACGGGACTT TTTGGTTGAA ATTTTAAAAG AAAAAAATTA TTTTTCTGAA 
AAAGAAAAAA CAACTATAGA CTTTGTGCAT CGAGTTGAGA AGTTTTATTC AGGACATTTC 
CAAGAAAATG TGCAGATCCA GTCCTTTCCC AGATATTGGA TAAAGGAAGA AGATTTTTTC 
AAGCACAAAT CTGTTTTGGA CTCATTCCTG AAACATGACC ATAAAGATAA ATTTAACAGA 
CAGGATCATA GAATTGTGTT AAATTGGGCC CAGTGGTGGA ATCGAATAAC ACAAGAATCC 
ATCCTTCTCC CCAGGATAAG TCCAGAAGAA TACAAATCAT GTTCTCCTGA AGCCCTCTGG 
AGGTACCTCA CAGCTCGCCA TGATTGGTTA AACATTGTCA CGTGGATTGG AGAATTTCAG 
ACCCAGAGTA GTGATTCTTC ACTTCAGCAG CACAAGTGGC CCCCTCTGAC TGTTGATGTT 
ATTGACCACA ACACTTGCTG CAGCAACTAC ATGAGGAATG AAATTTTAGA TAAGTTGGCC 
AGGAATGGGA TTTTTCTTAG ATCTGAACTA GAAGACTTTG AACTCTTCCT CTTAAGACTG 
AACCGTATTG GGGGTGTGAT GCAAGATACC CTCCCTGTTC AAAACTACAG GTCCAAAGAA 
GGTTGGGACT TCCATTCTCA CTTCATTCTC TATTGTTTGG AACGCAGTCT GCAGTATCTT 
CTTTATGTCT ATCTAGACTG TTACAAACTT AGTCCTCCTA ATTGTCCTTT TTTGGAAAGA 
AAAGAATTAC ATGAAGCTCA CCCGTGGTTT GAATTTTTAG TTCAGTGTCG ACAAGTTTCC 
AGTAATTTAA CAGATCCCAA ATTAATCTTC CAGGCTAGCC TTGCAAATGC TCAGATTCTG 
ATTCCCAGCA ACCAGGCCAG TGTGAGCAGT ATGCTATTGG AAGGACATAC CCTCCTGGCC 
CTTGCTACCA CAATGTATGC TCCTGGGGGT GTCAGCCAGG TTGTTCAGAA TGAAGAAAAT 
GAGAACTGTT TGAAGAAAGT AGACCCCCAG CTACTGAAGA TGGCATTAAC TCCTTACCCC 
AAGCTTAAAA CTGCTCTCTT CCCACAGTAC ACTGCCCCTA GTGTCCTGCC ACCCGACATT 
ACACTCTACC ACCTTCTTCA GTCGTTATTG CCCTTTGATC CCAGCAGATT GTTTGGCTGG 
CAGTCTGCCA ACACACTAGC TATAGGAGAT GCATCGAGTC ACCTCCCCCA TTTCTCTAGC 
CCAGACCTGG TTAATAAGTA TGCTATCGTG GAACGTCTGA ATTTTGCTTA TTATTTACAT 
CATGGACGGC CATCATTTGC ATTTGGTACT TTTCTGGTCC AGGAATTAAC CAAGAGCAAG 
ACTCCCAAGC AGCTGATCCA GCAAGTAGGC AATGAAGCCT ATGTTTTAGG CCTCTCCTCC 
TTCCATGTAC CTTCAATAGG AGCTGCCTGT GTTTGTTTCT TGGAATTGCT TGGCCTCGAC 
AGCCTCAAGC TCAGAGTTGA TATGAAAGTG GCCAATATAA TTTTGAGCTA CAAGTGTGGA 
AATGAAGATG CTCAGTACAG CTTTATCAGG GAGTCTCTAG CTGAAAAACT ATCTAAACTG 
GCTGCGGGTG AAAAGGCAAC CACAGAAGAA TTGCTTATTC TCTTAGAAGA GGGTATATGG 
AACAGCATTC AGCAGCAGGA AACAAAGAGA TTATCCAGTG AATCCAGCAG CCAATGGGCA 
TTAGTGGTAC AATTCTGCAG GCTCCACAAT GTGAAACTGA GCACGTCTTA CCTTATAGAG 
TGTGCCAAAG CAAATGACTG GTTACAGTTC ATTATTCATA GCCAGCTCCA TAATTACCAC 
CCAGAGGAGG TGAAATCTCT TCTCCAGTAT TTCAGCCCTG TCCTTCAAGA CCACTTACAG 
CTGGCTTTTG AGAACTTGCC CTCAGTGTCC AACTCCAGAA TGGACAATGA TCAAGTCTGC 
AACAGGTCCC CCCAGGAACT CCAGAGAAAC AAAGAAGATA CGACTGATTT CTTTAAAATT 
CTGCTGCAGT GCTCAGAGGA GCCAAACTCC TGGTGTTGGC TCCTAGCTGA AGCAGTGAAA 
CAACAGGCCC CTATCCTCAG TGTCCTGGCC TCGTGTCTCC AGGATTCCAG TGCTATTCCT 
TGTCTCTGTG TTTGGATCAT CACTTCTGTG GAGGACAGTG TTGCAGCTGA AGCAATGGGA 
CAAATTCAGG GTTCAACGGA GGGCCACACT TGGGACCTTG AGGACCTCTC TGTCATCTGG 
AGGACATTAT TAACGAGACA GAAGAGCAAA ACTCTCATCA GAGGTTTCCA ACTTTTCTTT 
AAGGATTCCC CGTTGCTATT GATGATGGAA ATGTATGAAC TGTGTATGTT CTTCAAGAAT 
TATAAGGAAG CCAAAGCAAA ACTATTGGAG TTCCAGAAGA ATCTTGAAAC TCTTGAAACA 
GCAGCCACAA AGGTCCACAC TGTCATCCCT GCCCCATGGC TGAAGGAGCA GGTGTGCTTT 
CTTTTGAAGC TTATGCTGCA GCAGTGTAGG ACCCAGTATG AGCTGGGGAA GCTTTTACAG 
CTGTTTGTTG GAGTAGAGCA TCTCTTCCCT GATGGTCCAG ATGTGAAGAA GCTGTGTGTC 
CTCAGCCAGA TTTTGAAAGA TACGTCCATA GCCATTAATC ATGTAATTAT TACCAGCTAC 
AGCCTTGAGA CTTTTCAGCA TGAATGTAGA TCTATCTTGG AAAAACTGGA GACAGATGGA 
CAGTTTGCTT TGGCCAGGAG AGTAGCAGAA TTAGCTGAGT TACCTGTGGA CAACTTGGTT 
ATTGAAGAGA TAACACAGGA AATGCAGACC TTAAAACACA TTGACCAGTG GTCTCTGAAA 
CAAGCAAAAA TTGACTTCTG GAAAAAATGC CATGAGAATT TTAAGAAAAA TTCCATTTCA 
AGCAAAGCAG CTTCTTCCTT TTTCTCAGCC CAGGCCCTTG TGGCATGTGC GTACCCGACT 
GAGGAGGGGC AGAGCAGCGT TGAGGAGCGC CATCTGCTGC TCACCCTGGC AGGGCACTGG 
CTTGCTCAGG AGGACCCGGT GCCTGTCAAC CAGCTGGAGG AGCTGGAGAA GCAGATCTGG 
CTGTGCCGCA TCACCCAGCA CACCCTCAGA GGAAGCCAGG GGGAAACAGA GCCCAGATTT 
TCTCAACAGA TCTCAACTAG TGGGGAACTT TCTTTTGATA GCTTAGCCAG TGAGTTTTCA 
TTCTCCAAGT TGGCTGCTCT GAACACATCA AAATACTTAG AACTTAATGG CCTTCCATCC 
AAAGAGACTT GTGAGAACAG ACTGGATTGG AAGGAGCAGG CGTCACTACA CTTTTTGATT 
GGGCGCCTCT TGGATGATGG CTGTGTGCAT GAAGCAAGTA GAGTGTGCCG GTATTTTCAT 
TTCTACAACC AAGATGTTGT CTTGGTATTG CACTGTAGAG CACTGGCTTC AGGTGAAGCT 
GGTGTGGATG ACCTGCATCC AGAGATCCAT GCTCTCCTAC AAAGTGCTGC GCTGCCTGAG 
GGAGAAGAAG AACCTGGCAT TCCTCTAAGG AAAGCCCAAA GCAACCTGTC TTTGCTCCCA 
GCTTCAAGTT TGGATAGTCA GTCGTTTGTG ATGCTGCCCT CAAGTGATGA AGTGGTGACT 
AATCTGAAAA CTCTGACTGG CAAATGCCTC CATGGGAAAA ACTACTGCCG GCAGGTCCTC 
TGTCTGTATG AACTTGCCAA GGAGTTGGGT TGTTCTTACA CAGATGTGGC TGCCCAGGAT 
GGTGAGGCCA TGCTCCGGAC AATCTTGGCC TCTCAGCAGC CTGACCGGTG CAAGCGAGCC 
CAAGCCTTCA TCAGCACCCA GGGCCTCGAG CCAGACACGG TGGCTGAACT TGTGGCCGAG 
GAGGTGACCA GAGAGCTGCT GACCCCATCA GAAAGAGCAG GACAGAAGCA GATGTTCACC 
CCAGCAGAGG AAAGCCAGAC TTTTCTTCCA CTGACCACTT TGTGTGCAGA CCGCACATTG 
GTAGGCATGA AGTTGTTGGA TAAGATTTCC TCCGTTCCCC ATGGGGAGTT GTCTTGCACC 
ACAGAGCTGC TGATCCTGGC CCATCACTGC TTCACGCTGA CATGCCACAT GGAGGGCATC 
ATCCGAGTCC TGCAGGCCGC CCGGCTGCTC ACAGACAACC ACTTGGCCCC CAATGAAGAG 
TACGGGCTGG TGGTGCGTCT CCTCACTGGC ATTGGAAGGT ACAATGAGAT GACATACATA 
TTTGATTTGC TGCATAAAAA GCACTACTTT GAAGTGCTGA TGAGGAAGAA ATTAGATCCG 
AGTGGTACCC TGAAGACAGC CCTACTCGAC TACATCAAAC GCTGCCGCCC TGGGGACAGT 
GAGAAGCACA ACATGATTGC CTTGTGCTTC AGCATGTGCC GTGAGATTGG GGAGAACCAT 
GAGGCAGCTG CCTGCATCCA GCTGAAATTG ATCGAGTCTC AGCCCTGGGA GGACAGCCTC 
AAAGATGGGC ACCAACTGAA GCAGCTGCTG CTGAAGGCCC TGACTCTGAT GCTGGATGCA 
GCAGAGAGTT ATGCCAAGGA CTCTTGCGTG CGACAGGCCC TGCACTGTCA TCGGCTCACC 
AAGTTGATAA CACTGCAGAT TCACTTTCTG AACACTGGCC AGAACACAAT GCTCATCAAC 
CTGAGCCGCC ACAGGCTGAT GGACTGTATC ATGACCCTAC CTCGGTTCTA CCAGGCTTCT 
ATTGTGGCGG AGGCCTATGA CTTTGTTCCT GATTGGGCTG AAATTCTATA CCAACGAGTG 
ATTCTTAAAG GAGACTTTAA TTACTTGGAA GAGTTTAAGC AGCAAAGGTT ATTAAAATCC 
AATATATTTG AAGAGATTTC AAAAAAATAT AAACAACATC AGCCTACCGA CACAGTTGTG 
AAAAACCTGA AGAAGTTACT CACGTATTGT GAAGATGTCT ACTTGTATTA CAAACTAGCT 
TATGAACACA AATTTTGTGA TATTGTAAAT GTGCTTCTGA AGGACCCTCA GATAGGGTGC 
TGTTTAAAGG ACATGCTAGC AGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023474271.1
CDS	507..7133
Translation	MCNEQQQEPAKISSFTSLKVSQDLDVVVIVSSSNSAIALNLNLYFRQHPGHLLCERTLEDSPIEGPKEIDEDDPANSDYNMKLTKFSFQVDRSWKAQLSSLNETIKSSKLEVSHCAPWFQDILHVEPSESGNHGTSVPGWAFIPQDIMGRQYNFPQKDHTKTNDPGRSWKVVHINEQEEPIKLKCVSVTGFTALFTWEAERMGCTIVLWDLETQGMQCCPLGKKCIPVDSSGDQQLCLVLTESGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLNPSSKSSVADQLDHFPSHLYLRNVEELTPALDLLCSAIRESDSETQSKHFSEQLLNLTLSFLNKQIKELFIHNEELDEHLQKGVNILTSYINELRTFMIKFPWKLTDAIDEYDANENVPKVKESHMWEKLSVEEVITNAVLNNKIPEAQTFFRINSHSAQRLEELIRIGLDLVFDSLKKNNIKEASELLKNMGFDVKDQLLKICFYTTDKNIRDFLVEILKEKNYFSEKEKTTIDFVHRVEKFYSGHFQENVQIQSFPRYWIKEEDFFKHKSVLDSFLKHDHKDKFNRQDHRIVLNWAQWWNRITQESILLPRISPEEYKSCSPEALWRYLTARHDWLNIVTWIGEFQTQSSDSSLQQHKWPPLTVDVIDHNTCCSNYMRNEILDKLARNGIFLRSELEDFELFLLRLNRIGGVMQDTLPVQNYRSKEGWDFHSHFILYCLERSLQYLLYVYLDCYKLSPPNCPFLERKELHEAHPWFEFLVQCRQVSSNLTDPKLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQNEENENCLKKVDPQLLKMALTPYPKLKTALFPQYTAPSVLPPDITLYHLLQSLLPFDPSRLFGWQSANTLAIGDASSHLPHFSSPDLVNKYAIVERLNFAYYLHHGRPSFAFGTFLVQELTKSKTPKQLIQQVGNEAYVLGLSSFHVPSIGAACVCFLELLGLDSLKLRVDMKVANIILSYKCGNEDAQYSFIRESLAEKLSKLAAGEKATTEELLILLEEGIWNSIQQQETKRLSSESSSQWALVVQFCRLHNVKLSTSYLIECAKANDWLQFIIHSQLHNYHPEEVKSLLQYFSPVLQDHLQLAFENLPSVSNSRMDNDQVCNRSPQELQRNKEDTTDFFKILLQCSEEPNSWCWLLAEAVKQQAPILSVLASCLQDSSAIPCLCVWIITSVEDSVAAEAMGQIQGSTEGHTWDLEDLSVIWRTLLTRQKSKTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAKAKLLEFQKNLETLETAATKVHTVIPAPWLKEQVCFLLKLMLQQCRTQYELGKLLQLFVGVEHLFPDGPDVKKLCVLSQILKDTSIAINHVIITSYSLETFQHECRSILEKLETDGQFALARRVAELAELPVDNLVIEEITQEMQTLKHIDQWSLKQAKIDFWKKCHENFKKNSISSKAASSFFSAQALVACAYPTEEGQSSVEERHLLLTLAGHWLAQEDPVPVNQLEELEKQIWLCRITQHTLRGSQGETEPRFSQQISTSGELSFDSLASEFSFSKLAALNTSKYLELNGLPSKETCENRLDWKEQASLHFLIGRLLDDGCVHEASRVCRYFHFYNQDVVLVLHCRALASGEAGVDDLHPEIHALLQSAALPEGEEEPGIPLRKAQSNLSLLPASSLDSQSFVMLPSSDEVVTNLKTLTGKCLHGKNYCRQVLCLYELAKELGCSYTDVAAQDGEAMLRTILASQQPDRCKRAQAFISTQGLEPDTVAELVAEEVTRELLTPSERAGQKQMFTPAEESQTFLPLTTLCADRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAARLLTDNHLAPNEEYGLVVRLLTGIGRYNEMTYIFDLLHKKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEDSLKDGHQLKQLLLKALTLMLDAAESYAKDSCVRQALHCHRLTKLITLQIHFLNTGQNTMLINLSRHRLMDCIMTLPRFYQASIVAEAYDFVPDWAEILYQRVILKGDFNYLEEFKQQRLLKSNIFEEISKKYKQHQPTDTVVKNLKKLLTYCEDVYLYYKLAYEHKFCDIVNVLLKDPQIGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_023618503.1
Organism	Equus caballus(horse)
Definition	Equus caballus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023618503.1

ORF Insert Sequence:

ATGTGTAATG AGCAACAACA GGAGCCAGCC AAGATTTCTT CTTTTACTTC ACTGAAAGTG 
TCTCAAGACC TAGATGTTGT AGTGATTGTC AGCTCCTCCA ACTCTGCAAT TGCTCTTAAC 
TTAAATTTGT ATTTCAGGCA ACACCCAGGA CACCTACTAT GCGAAAGAAC TCTAGAAGAC 
AGTCCTATTG AAGGCCCTAA GGAGATAGAT GAAGATGATC CTGCTAATTC TGACTACAAC 
ATGAAACTGA CCAAGTTTTC CTTCCAAGTT GATAGGTCTT GGAAAGCCCA GCTATCATCA 
TTGAATGAAA CAATAAAGAG CTCCAAGCTG GAGGTTTCCC ATTGTGCTCC ATGGTTCCAG 
GATATTTTAC ACGTGGAGCC TTCTGAATCT GGTAACCACG GTACCAGTGT GCCAGGCTGG 
GCCTTCATTC CACAGGACAT AATGGGCCGC CAGTATAATT TTCCACAGAA AGATCACACC 
AAGACCAATG ATCCAGGAAG ATCCTGGAAA GTAGTGCACA TCAATGAACA AGAGGAACCC 
ATCAAGCTTA AATGTGTGTC TGTGACAGGA TTCACTGCAC TGTTTACTTG GGAAGCAGAG 
AGGATGGGCT GCACCATTGT CCTTTGGGAT TTGGAGACCC AGGGCATGCA GTGTTGTCCT 
CTTGGCAAAA AATGTATTCC TGTAGACAGC AGTGGAGACC AGCAGCTGTG CCTTGTTTTG 
ACAGAAAGTG GACTTTCCCT GATTTTGTTT GGTTTGACTC AAGAGGAGTT TTTAAATAGA 
CTAATGATCC ATGGAAGTGC CAGCACTGTG GACTCTCTCT GCCATCTCAA TGGTTGGGGG 
AGGTGCTCAA TCCCCATACA TGCACTAGAG GCTGGCATAG AAAATCGTCA GCTGGACACA 
GTAGATTTCT TTTTGAAGAG CAAGGAAAAT CTTTTGAATC CATCCTCAAA ATCTTCTGTA 
GCTGATCAGC TTGACCACTT TCCATCCCAT TTGTATTTAA GAAATGTGGA AGAGCTGACA 
CCAGCATTGG ATTTACTTTG CTCAGCAATT AGAGAAAGTG ATTCTGAAAC CCAGAGCAAA 
CACTTCTCAG AACAGTTGCT TAATCTTACA CTGTCTTTCC TTAACAAACA AATAAAGGAG 
CTTTTTATTC ACAATGAAGA GCTAGATGAA CATCTACAGA AGGGAGTGAA CATTTTGACC 
AGCTACATTA ATGAACTTCG AACCTTCATG ATAAAGTTTC CTTGGAAGCT AACAGATGCC 
ATAGATGAAT ATGATGCAAA TGAAAATGTC CCCAAAGTAA AGGAGAGCCA TATGTGGGAG 
AAACTCAGCG TTGAGGAAGT TATCACCAAT GCCGTTTTAA ACAACAAAAT ACCAGAGGCA 
CAAACTTTCT TTAGGATTAA CAGTCATTCT GCTCAAAGAC TTGAGGAGCT GATTAGAATA 
GGCCTAGATT TGGTCTTTGA CAGTTTGAAA AAGAATAATA TAAAGGAAGC CTCCGAACTT 
TTGAAGAATA TGGGCTTTGA TGTAAAAGAC CAGCTGCTCA AGATATGCTT CTATACGACT 
GATAAAAATA TACGGGACTT TTTGGTTGAA ATTTTAAAAG AAAAAAATTA TTTTTCTGAA 
AAAGAAAAAA CAACTATAGA CTTTGTGCAT CGAGTTGAGA AGTTTTATTC AGGACATTTC 
CAAGAAAATG TGCAGATCCA GTCCTTTCCC AGATATTGGA TAAAGGAAGA AGATTTTTTC 
AAGCACAAAT CTGTTTTGGA CTCATTCCTG AAACATGACC ATAAAGATAA ATTTAACAGA 
CAGGATCATA GAATTGTGTT AAATTGGGCC CAGTGGTGGA ATCGAATAAC ACAAGAATCC 
ATCCTTCTCC CCAGGATAAG TCCAGAAGAA TACAAATCAT GTTCTCCTGA AGCCCTCTGG 
AGGTACCTCA CAGCTCGCCA TGATTGGTTA AACATTGTCA CGTGGATTGG AGAATTTCAG 
ACCCAGAGTA GTGATTCTTC ACTTCAGCAG CACAAGTGGC CCCCTCTGAC TGTTGATGTT 
ATTGACCACA ACACTTGCTG CAGCAACTAC ATGAGGAATG AAATTTTAGA TAAGTTGGCC 
AGGAATGGGA TTTTTCTTAG ATCTGAACTA GAAGACTTTG AACTCTTCCT CTTAAGACTG 
AACCGTATTG GGGGTGTGAT GCAAGATACC CTCCCTGTTC AAAACTACAG GTCCAAAGAA 
GGTTGGGACT TCCATTCTCA CTTCATTCTC TATTGTTTGG AACGCAGTCT GCAGTATCTT 
CTTTATGTCT ATCTAGACTG TTACAAACTT AGTCCTCCTA ATTGTCCTTT TTTGGAAAGA 
AAAGAATTAC ATGAAGCTCA CCCGTGGTTT GAATTTTTAG TTCAGTGTCG ACAAGTTTCC 
AGTAATTTAA CAGATCCCAA ATTAATCTTC CAGGCTAGCC TTGCAAATGC TCAGATTCTG 
ATTCCCAGCA ACCAGGCCAG TGTGAGCAGT ATGCTATTGG AAGGACATAC CCTCCTGGCC 
CTTGCTACCA CAATGTATGC TCCTGGGGGT GTCAGCCAGG TTGTTCAGAA TGAAGAAAAT 
GAGAACTGTT TGAAGAAAGT AGACCCCCAG CTACTGAAGA TGGCATTAAC TCCTTACCCC 
AAGCTTAAAA CTGCTCTCTT CCCACAGTAC ACTGCCCCTA GTGTCCTGCC ACCCGACATT 
ACACTCTACC ACCTTCTTCA GTCGTTATTG CCCTTTGATC CCAGCAGATT GTTTGGCTGG 
CAGTCTGCCA ACACACTAGC TATAGGAGAT GCATCGAGTC ACCTCCCCCA TTTCTCTAGC 
CCAGACCTGG TTAATAAGTA TGCTATCGTG GAACGTCTGA ATTTTGCTTA TTATTTACAT 
CATGGACGGC CATCATTTGC ATTTGGTACT TTTCTGGTCC AGGAATTAAC CAAGAGCAAG 
ACTCCCAAGC AGCTGATCCA GCAAGTAGGC AATGAAGCCT ATGTTTTAGG CCTCTCCTCC 
TTCCATGTAC CTTCAATAGG AGCTGCCTGT GTTTGTTTCT TGGAATTGCT TGGCCTCGAC 
AGCCTCAAGC TCAGAGTTGA TATGAAAGTG GCCAATATAA TTTTGAGCTA CAAGTGTGGA 
AATGAAGATG CTCAGTACAG CTTTATCAGG GAGTCTCTAG CTGAAAAACT ATCTAAACTG 
GCTGCGGGTG AAAAGGCAAC CACAGAAGAA TTGCTTATTC TCTTAGAAGA GGGTATATGG 
AACAGCATTC AGCAGCAGGA AACAAAGAGA TTATCCAGTG AATCCAGCAG CCAATGGGCA 
TTAGTGGTAC AATTCTGCAG GCTCCACAAT GTGAAACTGA GCACGTCTTA CCTTATAGAG 
TGTGCCAAAG CAAATGACTG GTTACAGTTC ATTATTCATA GCCAGCTCCA TAATTACCAC 
CCAGAGGAGG TGAAATCTCT TCTCCAGTAT TTCAGCCCTG TCCTTCAAGA CCACTTACAG 
CTGGCTTTTG AGAACTTGCC CTCAGTGTCC AACTCCAGAA TGGACAATGA TCAAGTCTGC 
AACAGGTCCC CCCAGGAACT CCAGAGAAAC AAAGAAGATA CGACTGATTT CTTTAAAATT 
CTGCTGCAGT GCTCAGAGGA GCCAAACTCC TGGTGTTGGC TCCTAGCTGA AGCAGTGAAA 
CAACAGGCCC CTATCCTCAG TGTCCTGGCC TCGTGTCTCC AGGATTCCAG TGCTATTCCT 
TGTCTCTGTG TTTGGATCAT CACTTCTGTG GAGGACAGTG TTGCAGCTGA AGCAATGGGA 
CAAATTCAGG GTTCAACGGA GGGCCACACT TGGGACCTTG AGGACCTCTC TGTCATCTGG 
AGGACATTAT TAACGAGACA GAAGAGCAAA ACTCTCATCA GAGGTTTCCA ACTTTTCTTT 
AAGGATTCCC CGTTGCTATT GATGATGGAA ATGTATGAAC TGTGTATGTT CTTCAAGAAT 
TATAAGGAAG CCAAAGCAAA ACTATTGGAG TTCCAGAAGA ATCTTGAAAC TCTTGAAACA 
GCAGCCACAA AGGTCCACAC TGTCATCCCT GCCCCATGGC TGAAGGAGCA GGTGTGCTTT 
CTTTTGAAGC TTATGCTGCA GCAGTGTAGG ACCCAGTATG AGCTGGGGAA GCTTTTACAG 
CTGTTTGTTG GAGTAGAGCA TCTCTTCCCT GATGGTCCAG ATGTGAAGAA GCTGTGTGTC 
CTCAGCCAGA TTTTGAAAGA TACGTCCATA GCCATTAATC ATGTAATTAT TACCAGCTAC 
AGCCTTGAGA CTTTTCAGCA TGAATGTAGA TCTATCTTGG AAAAACTGGA GACAGATGGA 
CAGTTTGCTT TGGCCAGGAG AGTAGCAGAA TTAGCTGAGT TACCTGTGGA CAACTTGGTT 
ATTGAAGAGA TAACACAGGA AATGCAGACC TTAAAACACA TTGACCAGTG GTCTCTGAAA 
CAAGCAAAAA TTGACTTCTG GAAAAAATGC CATGAGAATT TTAAGAAAAA TTCCATTTCA 
AGCAAAGCAG CTTCTTCCTT TTTCTCAGCC CAGGCCCTTG TGGCATGTGC GTACCCGACT 
GAGGAGGGGC AGAGCAGCGT TGAGGAGCGC CATCTGCTGC TCACCCTGGC AGGGCACTGG 
CTTGCTCAGG AGGACCCGGT GCCTGTCAAC CAGCTGGAGG AGCTGGAGAA GCAGATCTGG 
CTGTGCCGCA TCACCCAGCA CACCCTCAGA GGAAGCCAGG GGGAAACAGA GCCCAGATTT 
TCTCAACAGA TCTCAACTAG TGGGGAACTT TCTTTTGATA GCTTAGCCAG TGAGTTTTCA 
TTCTCCAAGT TGGCTGCTCT GAACACATCA AAATACTTAG AACTTAATGG CCTTCCATCC 
AAAGAGACTT GTGAGAACAG ACTGGATTGG AAGGAGCAGG CGTCACTACA CTTTTTGATT 
GGGCGCCTCT TGGATGATGG CTGTGTGCAT GAAGCAAGTA GAGTGTGCCG GTATTTTCAT 
TTCTACAACC AAGATGTTGT CTTGGTATTG CACTGTAGAG CACTGGCTTC AGGTGAAGCT 
GGTGTGGATG ACCTGCATCC AGAGATCCAT GCTCTCCTAC AAAGTGCTGC GCTGCCTGAG 
GGAGAAGAAG AACCTGGCAT TCCTCTAAGG AAAGCCCAAA GCAACCTGTC TTTGCTCCCA 
GCTTCAAGTT TGGATAGTCA GTCGTTTGTG ATGCTGCCCT CAAGTGATGA AGTGGTGACT 
AATCTGAAAA CTCTGACTGG CAAATGCCTC CATGGGAAAA ACTACTGCCG GCAGGTCCTC 
TGTCTGTATG AACTTGCCAA GGAGTTGGGT TGTTCTTACA CAGATGTGGC TGCCCAGGAT 
GGTGAGGCCA TGCTCCGGAC AATCTTGGCC TCTCAGCAGC CTGACCGGTG CAAGCGAGCC 
CAAGCCTTCA TCAGCACCCA GGGCCTCGAG CCAGACACGG TGGCTGAACT TGTGGCCGAG 
GAGGTGACCA GAGAGCTGCT GACCCCATCA GAAAGAGCAG GACAGAAGCA GATGTTCACC 
CCAGCAGAGG AAAGCCAGAC TTTTCTTCCA CTGACCACTT TGTGTGCAGA CCGCACATTG 
GTAGGCATGA AGTTGTTGGA TAAGATTTCC TCCGTTCCCC ATGGGGAGTT GTCTTGCACC 
ACAGAGCTGC TGATCCTGGC CCATCACTGC TTCACGCTGA CATGCCACAT GGAGGGCATC 
ATCCGAGTCC TGCAGGCCGC CCGGCTGCTC ACAGACAACC ACTTGGCCCC CAATGAAGAG 
TACGGGCTGG TGGTGCGTCT CCTCACTGGC ATTGGAAGGT ACAATGAGAT GACATACATA 
TTTGATTTGC TGCATAAAAA GCACTACTTT GAAGTGCTGA TGAGGAAGAA ATTAGATCCG 
AGTGGTACCC TGAAGACAGC CCTACTCGAC TACATCAAAC GCTGCCGCCC TGGGGACAGT 
GAGAAGCACA ACATGATTGC CTTGTGCTTC AGCATGTGCC GTGAGATTGG GGAGAACCAT 
GAGGCAGCTG CCTGCATCCA GCTGAAATTG ATCGAGTCTC AGCCCTGGGA GGACAGCCTC 
AAAGATGGGC ACCAACTGAA GCAGCTGCTG CTGAAGGCCC TGACTCTGAT GCTGGATGCA 
GCAGAGAGTT ATGCCAAGGA CTCTTGCGTG CGACAGGCCC TGCACTGTCA TCGGCTCACC 
AAGTTGATAA CACTGCAGAT TCACTTTCTG AACACTGGCC AGAACACAAT GCTCATCAAC 
CTGAGCCGCC ACAGGCTGAT GGACTGTATC ATGACCCTAC CTCGGTTCTA CCAGGCTTCT 
ATTGTGGCGG AGGCCTATGA CTTTGTTCCT GATTGGGCTG AAATTCTATA CCAACGAGTG 
ATTCTTAAAG GAGACTTTAA TTACTTGGAA GAGTTTAAGC AGCAAAGGTT ATTAAAATCC 
AATATATTTG AAGAGATTTC AAAAAAATAT AAACAACATC AGCCTACCGA CACAGTTGTG 
AAAAACCTGA AGAAGTTACT CACGTATTGT GAAGATGTCT ACTTGTATTA CAAACTAGCT 
TATGAACACA AATTTTGTGA TATTGTAAAT GTGCTTCTGA AGGACCCTCA GATAGGGTGC 
TGTTTAAAGG ACATGCTAGC AGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_023618497.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023474265.1 Equus caballus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X2, mRNA.

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CloneID	OHb42161
Clone ID Related Accession (Same CDS sequence)	XM_023618503.1 , XM_023618497.1
Accession Version	XM_023618497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTAATG AGCAACAACA GGAGCCAGCC AAGATTTCTT CTTTTACTTC ACTGAAAGTG 
TCTCAAGACC TAGATGTTGT AGTGATTGTC AGCTCCTCCA ACTCTGCAAT TGCTCTTAAC 
TTAAATTTGT ATTTCAGGCA ACACCCAGGA CACCTACTAT GCGAAAGAAC TCTAGAAGAC 
AGTCCTATTG AAGGCCCTAA GGAGATAGAT GAAGATGATC CTGCTAATTC TGACTACAAC 
ATGAAACTGA CCAAGTTTTC CTTCCAAGTT GATAGGTCTT GGAAAGCCCA GCTATCATCA 
TTGAATGAAA CAATAAAGAG CTCCAAGCTG GAGGTTTCCC ATTGTGCTCC ATGGTTCCAG 
GATATTTTAC ACGTGGAGCC TTCTGAATCT GGTAACCACG GTACCAGTGT GCCAGGCTGG 
GCCTTCATTC CACAGGACAT AATGGGCCGC CAGTATAATT TTCCACAGAA AGATCACACC 
AAGACCAATG ATCCAGGAAG ATCCTGGAAA GTAGTGCACA TCAATGAACA AGAGGAACCC 
ATCAAGCTTA AATGTGTGTC TGTGACAGGA TTCACTGCAC TGTTTACTTG GGAAGCAGAG 
AGGATGGGCT GCACCATTGT CCTTTGGGAT TTGGAGACCC AGGGCATGCA GTGTTGTCCT 
CTTGGCAAAA AATGTATTCC TGTAGACAGC AGTGGAGACC AGCAGCTGTG CCTTGTTTTG 
ACAGAAAGTG GACTTTCCCT GATTTTGTTT GGTTTGACTC AAGAGGAGTT TTTAAATAGA 
CTAATGATCC ATGGAAGTGC CAGCACTGTG GACTCTCTCT GCCATCTCAA TGGTTGGGGG 
AGGTGCTCAA TCCCCATACA TGCACTAGAG GCTGGCATAG AAAATCGTCA GCTGGACACA 
GTAGATTTCT TTTTGAAGAG CAAGGAAAAT CTTTTGAATC CATCCTCAAA ATCTTCTGTA 
GCTGATCAGC TTGACCACTT TCCATCCCAT TTGTATTTAA GAAATGTGGA AGAGCTGACA 
CCAGCATTGG ATTTACTTTG CTCAGCAATT AGAGAAAGTG ATTCTGAAAC CCAGAGCAAA 
CACTTCTCAG AACAGTTGCT TAATCTTACA CTGTCTTTCC TTAACAAACA AATAAAGGAG 
CTTTTTATTC ACAATGAAGA GCTAGATGAA CATCTACAGA AGGGAGTGAA CATTTTGACC 
AGCTACATTA ATGAACTTCG AACCTTCATG ATAAAGTTTC CTTGGAAGCT AACAGATGCC 
ATAGATGAAT ATGATGCAAA TGAAAATGTC CCCAAAGTAA AGGAGAGCCA TATGTGGGAG 
AAACTCAGCG TTGAGGAAGT TATCACCAAT GCCGTTTTAA ACAACAAAAT ACCAGAGGCA 
CAAACTTTCT TTAGGATTAA CAGTCATTCT GCTCAAAGAC TTGAGGAGCT GATTAGAATA 
GGCCTAGATT TGGTCTTTGA CAGTTTGAAA AAGAATAATA TAAAGGAAGC CTCCGAACTT 
TTGAAGAATA TGGGCTTTGA TGTAAAAGAC CAGCTGCTCA AGATATGCTT CTATACGACT 
GATAAAAATA TACGGGACTT TTTGGTTGAA ATTTTAAAAG AAAAAAATTA TTTTTCTGAA 
AAAGAAAAAA CAACTATAGA CTTTGTGCAT CGAGTTGAGA AGTTTTATTC AGGACATTTC 
CAAGAAAATG TGCAGATCCA GTCCTTTCCC AGATATTGGA TAAAGGAAGA AGATTTTTTC 
AAGCACAAAT CTGTTTTGGA CTCATTCCTG AAACATGACC ATAAAGATAA ATTTAACAGA 
CAGGATCATA GAATTGTGTT AAATTGGGCC CAGTGGTGGA ATCGAATAAC ACAAGAATCC 
ATCCTTCTCC CCAGGATAAG TCCAGAAGAA TACAAATCAT GTTCTCCTGA AGCCCTCTGG 
AGGTACCTCA CAGCTCGCCA TGATTGGTTA AACATTGTCA CGTGGATTGG AGAATTTCAG 
ACCCAGAGTA GTGATTCTTC ACTTCAGCAG CACAAGTGGC CCCCTCTGAC TGTTGATGTT 
ATTGACCACA ACACTTGCTG CAGCAACTAC ATGAGGAATG AAATTTTAGA TAAGTTGGCC 
AGGAATGGGA TTTTTCTTAG ATCTGAACTA GAAGACTTTG AACTCTTCCT CTTAAGACTG 
AACCGTATTG GGGGTGTGAT GCAAGATACC CTCCCTGTTC AAAACTACAG GTCCAAAGAA 
GGTTGGGACT TCCATTCTCA CTTCATTCTC TATTGTTTGG AACGCAGTCT GCAGTATCTT 
CTTTATGTCT ATCTAGACTG TTACAAACTT AGTCCTCCTA ATTGTCCTTT TTTGGAAAGA 
AAAGAATTAC ATGAAGCTCA CCCGTGGTTT GAATTTTTAG TTCAGTGTCG ACAAGTTTCC 
AGTAATTTAA CAGATCCCAA ATTAATCTTC CAGGCTAGCC TTGCAAATGC TCAGATTCTG 
ATTCCCAGCA ACCAGGCCAG TGTGAGCAGT ATGCTATTGG AAGGACATAC CCTCCTGGCC 
CTTGCTACCA CAATGTATGC TCCTGGGGGT GTCAGCCAGG TTGTTCAGAA TGAAGAAAAT 
GAGAACTGTT TGAAGAAAGT AGACCCCCAG CTACTGAAGA TGGCATTAAC TCCTTACCCC 
AAGCTTAAAA CTGCTCTCTT CCCACAGTAC ACTGCCCCTA GTGTCCTGCC ACCCGACATT 
ACACTCTACC ACCTTCTTCA GTCGTTATTG CCCTTTGATC CCAGCAGATT GTTTGGCTGG 
CAGTCTGCCA ACACACTAGC TATAGGAGAT GCATCGAGTC ACCTCCCCCA TTTCTCTAGC 
CCAGACCTGG TTAATAAGTA TGCTATCGTG GAACGTCTGA ATTTTGCTTA TTATTTACAT 
CATGGACGGC CATCATTTGC ATTTGGTACT TTTCTGGTCC AGGAATTAAC CAAGAGCAAG 
ACTCCCAAGC AGCTGATCCA GCAAGTAGGC AATGAAGCCT ATGTTTTAGG CCTCTCCTCC 
TTCCATGTAC CTTCAATAGG AGCTGCCTGT GTTTGTTTCT TGGAATTGCT TGGCCTCGAC 
AGCCTCAAGC TCAGAGTTGA TATGAAAGTG GCCAATATAA TTTTGAGCTA CAAGTGTGGA 
AATGAAGATG CTCAGTACAG CTTTATCAGG GAGTCTCTAG CTGAAAAACT ATCTAAACTG 
GCTGCGGGTG AAAAGGCAAC CACAGAAGAA TTGCTTATTC TCTTAGAAGA GGGTATATGG 
AACAGCATTC AGCAGCAGGA AACAAAGAGA TTATCCAGTG AATCCAGCAG CCAATGGGCA 
TTAGTGGTAC AATTCTGCAG GCTCCACAAT GTGAAACTGA GCACGTCTTA CCTTATAGAG 
TGTGCCAAAG CAAATGACTG GTTACAGTTC ATTATTCATA GCCAGCTCCA TAATTACCAC 
CCAGAGGAGG TGAAATCTCT TCTCCAGTAT TTCAGCCCTG TCCTTCAAGA CCACTTACAG 
CTGGCTTTTG AGAACTTGCC CTCAGTGTCC AACTCCAGAA TGGACAATGA TCAAGTCTGC 
AACAGGTCCC CCCAGGAACT CCAGAGAAAC AAAGAAGATA CGACTGATTT CTTTAAAATT 
CTGCTGCAGT GCTCAGAGGA GCCAAACTCC TGGTGTTGGC TCCTAGCTGA AGCAGTGAAA 
CAACAGGCCC CTATCCTCAG TGTCCTGGCC TCGTGTCTCC AGGATTCCAG TGCTATTCCT 
TGTCTCTGTG TTTGGATCAT CACTTCTGTG GAGGACAGTG TTGCAGCTGA AGCAATGGGA 
CAAATTCAGG GTTCAACGGA GGGCCACACT TGGGACCTTG AGGACCTCTC TGTCATCTGG 
AGGACATTAT TAACGAGACA GAAGAGCAAA ACTCTCATCA GAGGTTTCCA ACTTTTCTTT 
AAGGATTCCC CGTTGCTATT GATGATGGAA ATGTATGAAC TGTGTATGTT CTTCAAGAAT 
TATAAGGAAG CCAAAGCAAA ACTATTGGAG TTCCAGAAGA ATCTTGAAAC TCTTGAAACA 
GCAGCCACAA AGGTCCACAC TGTCATCCCT GCCCCATGGC TGAAGGAGCA GGTGTGCTTT 
CTTTTGAAGC TTATGCTGCA GCAGTGTAGG ACCCAGTATG AGCTGGGGAA GCTTTTACAG 
CTGTTTGTTG GAGTAGAGCA TCTCTTCCCT GATGGTCCAG ATGTGAAGAA GCTGTGTGTC 
CTCAGCCAGA TTTTGAAAGA TACGTCCATA GCCATTAATC ATGTAATTAT TACCAGCTAC 
AGCCTTGAGA CTTTTCAGCA TGAATGTAGA TCTATCTTGG AAAAACTGGA GACAGATGGA 
CAGTTTGCTT TGGCCAGGAG AGTAGCAGAA TTAGCTGAGT TACCTGTGGA CAACTTGGTT 
ATTGAAGAGA TAACACAGGA AATGCAGACC TTAAAACACA TTGACCAGTG GTCTCTGAAA 
CAAGCAAAAA TTGACTTCTG GAAAAAATGC CATGAGAATT TTAAGAAAAA TTCCATTTCA 
AGCAAAGCAG CTTCTTCCTT TTTCTCAGCC CAGGCCCTTG TGGCATGTGC GTACCCGACT 
GAGGAGGGGC AGAGCAGCGT TGAGGAGCGC CATCTGCTGC TCACCCTGGC AGGGCACTGG 
CTTGCTCAGG AGGACCCGGT GCCTGTCAAC CAGCTGGAGG AGCTGGAGAA GCAGATCTGG 
CTGTGCCGCA TCACCCAGCA CACCCTCAGA GGAAGCCAGG GGGAAACAGA GCCCAGATTT 
TCTCAACAGA TCTCAACTAG TGGGGAACTT TCTTTTGATA GCTTAGCCAG TGAGTTTTCA 
TTCTCCAAGT TGGCTGCTCT GAACACATCA AAATACTTAG AACTTAATGG CCTTCCATCC 
AAAGAGACTT GTGAGAACAG ACTGGATTGG AAGGAGCAGG CGTCACTACA CTTTTTGATT 
GGGCGCCTCT TGGATGATGG CTGTGTGCAT GAAGCAAGTA GAGTGTGCCG GTATTTTCAT 
TTCTACAACC AAGATGTTGT CTTGGTATTG CACTGTAGAG CACTGGCTTC AGGTGAAGCT 
GGTGTGGATG ACCTGCATCC AGAGATCCAT GCTCTCCTAC AAAGTGCTGC GCTGCCTGAG 
GGAGAAGAAG AACCTGGCAT TCCTCTAAGG AAAGCCCAAA GCAACCTGTC TTTGCTCCCA 
GCTTCAAGTT TGGATAGTCA GTCGTTTGTG ATGCTGCCCT CAAGTGATGA AGTGGTGACT 
AATCTGAAAA CTCTGACTGG CAAATGCCTC CATGGGAAAA ACTACTGCCG GCAGGTCCTC 
TGTCTGTATG AACTTGCCAA GGAGTTGGGT TGTTCTTACA CAGATGTGGC TGCCCAGGAT 
GGTGAGGCCA TGCTCCGGAC AATCTTGGCC TCTCAGCAGC CTGACCGGTG CAAGCGAGCC 
CAAGCCTTCA TCAGCACCCA GGGCCTCGAG CCAGACACGG TGGCTGAACT TGTGGCCGAG 
GAGGTGACCA GAGAGCTGCT GACCCCATCA GAAAGAGCAG GACAGAAGCA GATGTTCACC 
CCAGCAGAGG AAAGCCAGAC TTTTCTTCCA CTGACCACTT TGTGTGCAGA CCGCACATTG 
GTAGGCATGA AGTTGTTGGA TAAGATTTCC TCCGTTCCCC ATGGGGAGTT GTCTTGCACC 
ACAGAGCTGC TGATCCTGGC CCATCACTGC TTCACGCTGA CATGCCACAT GGAGGGCATC 
ATCCGAGTCC TGCAGGCCGC CCGGCTGCTC ACAGACAACC ACTTGGCCCC CAATGAAGAG 
TACGGGCTGG TGGTGCGTCT CCTCACTGGC ATTGGAAGGT ACAATGAGAT GACATACATA 
TTTGATTTGC TGCATAAAAA GCACTACTTT GAAGTGCTGA TGAGGAAGAA ATTAGATCCG 
AGTGGTACCC TGAAGACAGC CCTACTCGAC TACATCAAAC GCTGCCGCCC TGGGGACAGT 
GAGAAGCACA ACATGATTGC CTTGTGCTTC AGCATGTGCC GTGAGATTGG GGAGAACCAT 
GAGGCAGCTG CCTGCATCCA GCTGAAATTG ATCGAGTCTC AGCCCTGGGA GGACAGCCTC 
AAAGATGGGC ACCAACTGAA GCAGCTGCTG CTGAAGGCCC TGACTCTGAT GCTGGATGCA 
GCAGAGAGTT ATGCCAAGGA CTCTTGCGTG CGACAGGCCC TGCACTGTCA TCGGCTCACC 
AAGTTGATAA CACTGCAGAT TCACTTTCTG AACACTGGCC AGAACACAAT GCTCATCAAC 
CTGAGCCGCC ACAGGCTGAT GGACTGTATC ATGACCCTAC CTCGGTTCTA CCAGGCTTCT 
ATTGTGGCGG AGGCCTATGA CTTTGTTCCT GATTGGGCTG AAATTCTATA CCAACGAGTG 
ATTCTTAAAG GAGACTTTAA TTACTTGGAA GAGTTTAAGC AGCAAAGGTT ATTAAAATCC 
AATATATTTG AAGAGATTTC AAAAAAATAT AAACAACATC AGCCTACCGA CACAGTTGTG 
AAAAACCTGA AGAAGTTACT CACGTATTGT GAAGATGTCT ACTTGTATTA CAAACTAGCT 
TATGAACACA AATTTTGTGA TATTGTAAAT GTGCTTCTGA AGGACCCTCA GATAGGGTGC 
TGTTTAAAGG ACATGCTAGC AGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023474265.1
CDS	1299..7925
Translation	MCNEQQQEPAKISSFTSLKVSQDLDVVVIVSSSNSAIALNLNLYFRQHPGHLLCERTLEDSPIEGPKEIDEDDPANSDYNMKLTKFSFQVDRSWKAQLSSLNETIKSSKLEVSHCAPWFQDILHVEPSESGNHGTSVPGWAFIPQDIMGRQYNFPQKDHTKTNDPGRSWKVVHINEQEEPIKLKCVSVTGFTALFTWEAERMGCTIVLWDLETQGMQCCPLGKKCIPVDSSGDQQLCLVLTESGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLNPSSKSSVADQLDHFPSHLYLRNVEELTPALDLLCSAIRESDSETQSKHFSEQLLNLTLSFLNKQIKELFIHNEELDEHLQKGVNILTSYINELRTFMIKFPWKLTDAIDEYDANENVPKVKESHMWEKLSVEEVITNAVLNNKIPEAQTFFRINSHSAQRLEELIRIGLDLVFDSLKKNNIKEASELLKNMGFDVKDQLLKICFYTTDKNIRDFLVEILKEKNYFSEKEKTTIDFVHRVEKFYSGHFQENVQIQSFPRYWIKEEDFFKHKSVLDSFLKHDHKDKFNRQDHRIVLNWAQWWNRITQESILLPRISPEEYKSCSPEALWRYLTARHDWLNIVTWIGEFQTQSSDSSLQQHKWPPLTVDVIDHNTCCSNYMRNEILDKLARNGIFLRSELEDFELFLLRLNRIGGVMQDTLPVQNYRSKEGWDFHSHFILYCLERSLQYLLYVYLDCYKLSPPNCPFLERKELHEAHPWFEFLVQCRQVSSNLTDPKLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQNEENENCLKKVDPQLLKMALTPYPKLKTALFPQYTAPSVLPPDITLYHLLQSLLPFDPSRLFGWQSANTLAIGDASSHLPHFSSPDLVNKYAIVERLNFAYYLHHGRPSFAFGTFLVQELTKSKTPKQLIQQVGNEAYVLGLSSFHVPSIGAACVCFLELLGLDSLKLRVDMKVANIILSYKCGNEDAQYSFIRESLAEKLSKLAAGEKATTEELLILLEEGIWNSIQQQETKRLSSESSSQWALVVQFCRLHNVKLSTSYLIECAKANDWLQFIIHSQLHNYHPEEVKSLLQYFSPVLQDHLQLAFENLPSVSNSRMDNDQVCNRSPQELQRNKEDTTDFFKILLQCSEEPNSWCWLLAEAVKQQAPILSVLASCLQDSSAIPCLCVWIITSVEDSVAAEAMGQIQGSTEGHTWDLEDLSVIWRTLLTRQKSKTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAKAKLLEFQKNLETLETAATKVHTVIPAPWLKEQVCFLLKLMLQQCRTQYELGKLLQLFVGVEHLFPDGPDVKKLCVLSQILKDTSIAINHVIITSYSLETFQHECRSILEKLETDGQFALARRVAELAELPVDNLVIEEITQEMQTLKHIDQWSLKQAKIDFWKKCHENFKKNSISSKAASSFFSAQALVACAYPTEEGQSSVEERHLLLTLAGHWLAQEDPVPVNQLEELEKQIWLCRITQHTLRGSQGETEPRFSQQISTSGELSFDSLASEFSFSKLAALNTSKYLELNGLPSKETCENRLDWKEQASLHFLIGRLLDDGCVHEASRVCRYFHFYNQDVVLVLHCRALASGEAGVDDLHPEIHALLQSAALPEGEEEPGIPLRKAQSNLSLLPASSLDSQSFVMLPSSDEVVTNLKTLTGKCLHGKNYCRQVLCLYELAKELGCSYTDVAAQDGEAMLRTILASQQPDRCKRAQAFISTQGLEPDTVAELVAEEVTRELLTPSERAGQKQMFTPAEESQTFLPLTTLCADRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAARLLTDNHLAPNEEYGLVVRLLTGIGRYNEMTYIFDLLHKKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEDSLKDGHQLKQLLLKALTLMLDAAESYAKDSCVRQALHCHRLTKLITLQIHFLNTGQNTMLINLSRHRLMDCIMTLPRFYQASIVAEAYDFVPDWAEILYQRVILKGDFNYLEEFKQQRLLKSNIFEEISKKYKQHQPTDTVVKNLKKLLTYCEDVYLYYKLAYEHKFCDIVNVLLKDPQIGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_023618497.1
Organism	Equus caballus(horse)
Definition	Equus caballus SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023618497.1

ORF Insert Sequence:

ATGTGTAATG AGCAACAACA GGAGCCAGCC AAGATTTCTT CTTTTACTTC ACTGAAAGTG 
TCTCAAGACC TAGATGTTGT AGTGATTGTC AGCTCCTCCA ACTCTGCAAT TGCTCTTAAC 
TTAAATTTGT ATTTCAGGCA ACACCCAGGA CACCTACTAT GCGAAAGAAC TCTAGAAGAC 
AGTCCTATTG AAGGCCCTAA GGAGATAGAT GAAGATGATC CTGCTAATTC TGACTACAAC 
ATGAAACTGA CCAAGTTTTC CTTCCAAGTT GATAGGTCTT GGAAAGCCCA GCTATCATCA 
TTGAATGAAA CAATAAAGAG CTCCAAGCTG GAGGTTTCCC ATTGTGCTCC ATGGTTCCAG 
GATATTTTAC ACGTGGAGCC TTCTGAATCT GGTAACCACG GTACCAGTGT GCCAGGCTGG 
GCCTTCATTC CACAGGACAT AATGGGCCGC CAGTATAATT TTCCACAGAA AGATCACACC 
AAGACCAATG ATCCAGGAAG ATCCTGGAAA GTAGTGCACA TCAATGAACA AGAGGAACCC 
ATCAAGCTTA AATGTGTGTC TGTGACAGGA TTCACTGCAC TGTTTACTTG GGAAGCAGAG 
AGGATGGGCT GCACCATTGT CCTTTGGGAT TTGGAGACCC AGGGCATGCA GTGTTGTCCT 
CTTGGCAAAA AATGTATTCC TGTAGACAGC AGTGGAGACC AGCAGCTGTG CCTTGTTTTG 
ACAGAAAGTG GACTTTCCCT GATTTTGTTT GGTTTGACTC AAGAGGAGTT TTTAAATAGA 
CTAATGATCC ATGGAAGTGC CAGCACTGTG GACTCTCTCT GCCATCTCAA TGGTTGGGGG 
AGGTGCTCAA TCCCCATACA TGCACTAGAG GCTGGCATAG AAAATCGTCA GCTGGACACA 
GTAGATTTCT TTTTGAAGAG CAAGGAAAAT CTTTTGAATC CATCCTCAAA ATCTTCTGTA 
GCTGATCAGC TTGACCACTT TCCATCCCAT TTGTATTTAA GAAATGTGGA AGAGCTGACA 
CCAGCATTGG ATTTACTTTG CTCAGCAATT AGAGAAAGTG ATTCTGAAAC CCAGAGCAAA 
CACTTCTCAG AACAGTTGCT TAATCTTACA CTGTCTTTCC TTAACAAACA AATAAAGGAG 
CTTTTTATTC ACAATGAAGA GCTAGATGAA CATCTACAGA AGGGAGTGAA CATTTTGACC 
AGCTACATTA ATGAACTTCG AACCTTCATG ATAAAGTTTC CTTGGAAGCT AACAGATGCC 
ATAGATGAAT ATGATGCAAA TGAAAATGTC CCCAAAGTAA AGGAGAGCCA TATGTGGGAG 
AAACTCAGCG TTGAGGAAGT TATCACCAAT GCCGTTTTAA ACAACAAAAT ACCAGAGGCA 
CAAACTTTCT TTAGGATTAA CAGTCATTCT GCTCAAAGAC TTGAGGAGCT GATTAGAATA 
GGCCTAGATT TGGTCTTTGA CAGTTTGAAA AAGAATAATA TAAAGGAAGC CTCCGAACTT 
TTGAAGAATA TGGGCTTTGA TGTAAAAGAC CAGCTGCTCA AGATATGCTT CTATACGACT 
GATAAAAATA TACGGGACTT TTTGGTTGAA ATTTTAAAAG AAAAAAATTA TTTTTCTGAA 
AAAGAAAAAA CAACTATAGA CTTTGTGCAT CGAGTTGAGA AGTTTTATTC AGGACATTTC 
CAAGAAAATG TGCAGATCCA GTCCTTTCCC AGATATTGGA TAAAGGAAGA AGATTTTTTC 
AAGCACAAAT CTGTTTTGGA CTCATTCCTG AAACATGACC ATAAAGATAA ATTTAACAGA 
CAGGATCATA GAATTGTGTT AAATTGGGCC CAGTGGTGGA ATCGAATAAC ACAAGAATCC 
ATCCTTCTCC CCAGGATAAG TCCAGAAGAA TACAAATCAT GTTCTCCTGA AGCCCTCTGG 
AGGTACCTCA CAGCTCGCCA TGATTGGTTA AACATTGTCA CGTGGATTGG AGAATTTCAG 
ACCCAGAGTA GTGATTCTTC ACTTCAGCAG CACAAGTGGC CCCCTCTGAC TGTTGATGTT 
ATTGACCACA ACACTTGCTG CAGCAACTAC ATGAGGAATG AAATTTTAGA TAAGTTGGCC 
AGGAATGGGA TTTTTCTTAG ATCTGAACTA GAAGACTTTG AACTCTTCCT CTTAAGACTG 
AACCGTATTG GGGGTGTGAT GCAAGATACC CTCCCTGTTC AAAACTACAG GTCCAAAGAA 
GGTTGGGACT TCCATTCTCA CTTCATTCTC TATTGTTTGG AACGCAGTCT GCAGTATCTT 
CTTTATGTCT ATCTAGACTG TTACAAACTT AGTCCTCCTA ATTGTCCTTT TTTGGAAAGA 
AAAGAATTAC ATGAAGCTCA CCCGTGGTTT GAATTTTTAG TTCAGTGTCG ACAAGTTTCC 
AGTAATTTAA CAGATCCCAA ATTAATCTTC CAGGCTAGCC TTGCAAATGC TCAGATTCTG 
ATTCCCAGCA ACCAGGCCAG TGTGAGCAGT ATGCTATTGG AAGGACATAC CCTCCTGGCC 
CTTGCTACCA CAATGTATGC TCCTGGGGGT GTCAGCCAGG TTGTTCAGAA TGAAGAAAAT 
GAGAACTGTT TGAAGAAAGT AGACCCCCAG CTACTGAAGA TGGCATTAAC TCCTTACCCC 
AAGCTTAAAA CTGCTCTCTT CCCACAGTAC ACTGCCCCTA GTGTCCTGCC ACCCGACATT 
ACACTCTACC ACCTTCTTCA GTCGTTATTG CCCTTTGATC CCAGCAGATT GTTTGGCTGG 
CAGTCTGCCA ACACACTAGC TATAGGAGAT GCATCGAGTC ACCTCCCCCA TTTCTCTAGC 
CCAGACCTGG TTAATAAGTA TGCTATCGTG GAACGTCTGA ATTTTGCTTA TTATTTACAT 
CATGGACGGC CATCATTTGC ATTTGGTACT TTTCTGGTCC AGGAATTAAC CAAGAGCAAG 
ACTCCCAAGC AGCTGATCCA GCAAGTAGGC AATGAAGCCT ATGTTTTAGG CCTCTCCTCC 
TTCCATGTAC CTTCAATAGG AGCTGCCTGT GTTTGTTTCT TGGAATTGCT TGGCCTCGAC 
AGCCTCAAGC TCAGAGTTGA TATGAAAGTG GCCAATATAA TTTTGAGCTA CAAGTGTGGA 
AATGAAGATG CTCAGTACAG CTTTATCAGG GAGTCTCTAG CTGAAAAACT ATCTAAACTG 
GCTGCGGGTG AAAAGGCAAC CACAGAAGAA TTGCTTATTC TCTTAGAAGA GGGTATATGG 
AACAGCATTC AGCAGCAGGA AACAAAGAGA TTATCCAGTG AATCCAGCAG CCAATGGGCA 
TTAGTGGTAC AATTCTGCAG GCTCCACAAT GTGAAACTGA GCACGTCTTA CCTTATAGAG 
TGTGCCAAAG CAAATGACTG GTTACAGTTC ATTATTCATA GCCAGCTCCA TAATTACCAC 
CCAGAGGAGG TGAAATCTCT TCTCCAGTAT TTCAGCCCTG TCCTTCAAGA CCACTTACAG 
CTGGCTTTTG AGAACTTGCC CTCAGTGTCC AACTCCAGAA TGGACAATGA TCAAGTCTGC 
AACAGGTCCC CCCAGGAACT CCAGAGAAAC AAAGAAGATA CGACTGATTT CTTTAAAATT 
CTGCTGCAGT GCTCAGAGGA GCCAAACTCC TGGTGTTGGC TCCTAGCTGA AGCAGTGAAA 
CAACAGGCCC CTATCCTCAG TGTCCTGGCC TCGTGTCTCC AGGATTCCAG TGCTATTCCT 
TGTCTCTGTG TTTGGATCAT CACTTCTGTG GAGGACAGTG TTGCAGCTGA AGCAATGGGA 
CAAATTCAGG GTTCAACGGA GGGCCACACT TGGGACCTTG AGGACCTCTC TGTCATCTGG 
AGGACATTAT TAACGAGACA GAAGAGCAAA ACTCTCATCA GAGGTTTCCA ACTTTTCTTT 
AAGGATTCCC CGTTGCTATT GATGATGGAA ATGTATGAAC TGTGTATGTT CTTCAAGAAT 
TATAAGGAAG CCAAAGCAAA ACTATTGGAG TTCCAGAAGA ATCTTGAAAC TCTTGAAACA 
GCAGCCACAA AGGTCCACAC TGTCATCCCT GCCCCATGGC TGAAGGAGCA GGTGTGCTTT 
CTTTTGAAGC TTATGCTGCA GCAGTGTAGG ACCCAGTATG AGCTGGGGAA GCTTTTACAG 
CTGTTTGTTG GAGTAGAGCA TCTCTTCCCT GATGGTCCAG ATGTGAAGAA GCTGTGTGTC 
CTCAGCCAGA TTTTGAAAGA TACGTCCATA GCCATTAATC ATGTAATTAT TACCAGCTAC 
AGCCTTGAGA CTTTTCAGCA TGAATGTAGA TCTATCTTGG AAAAACTGGA GACAGATGGA 
CAGTTTGCTT TGGCCAGGAG AGTAGCAGAA TTAGCTGAGT TACCTGTGGA CAACTTGGTT 
ATTGAAGAGA TAACACAGGA AATGCAGACC TTAAAACACA TTGACCAGTG GTCTCTGAAA 
CAAGCAAAAA TTGACTTCTG GAAAAAATGC CATGAGAATT TTAAGAAAAA TTCCATTTCA 
AGCAAAGCAG CTTCTTCCTT TTTCTCAGCC CAGGCCCTTG TGGCATGTGC GTACCCGACT 
GAGGAGGGGC AGAGCAGCGT TGAGGAGCGC CATCTGCTGC TCACCCTGGC AGGGCACTGG 
CTTGCTCAGG AGGACCCGGT GCCTGTCAAC CAGCTGGAGG AGCTGGAGAA GCAGATCTGG 
CTGTGCCGCA TCACCCAGCA CACCCTCAGA GGAAGCCAGG GGGAAACAGA GCCCAGATTT 
TCTCAACAGA TCTCAACTAG TGGGGAACTT TCTTTTGATA GCTTAGCCAG TGAGTTTTCA 
TTCTCCAAGT TGGCTGCTCT GAACACATCA AAATACTTAG AACTTAATGG CCTTCCATCC 
AAAGAGACTT GTGAGAACAG ACTGGATTGG AAGGAGCAGG CGTCACTACA CTTTTTGATT 
GGGCGCCTCT TGGATGATGG CTGTGTGCAT GAAGCAAGTA GAGTGTGCCG GTATTTTCAT 
TTCTACAACC AAGATGTTGT CTTGGTATTG CACTGTAGAG CACTGGCTTC AGGTGAAGCT 
GGTGTGGATG ACCTGCATCC AGAGATCCAT GCTCTCCTAC AAAGTGCTGC GCTGCCTGAG 
GGAGAAGAAG AACCTGGCAT TCCTCTAAGG AAAGCCCAAA GCAACCTGTC TTTGCTCCCA 
GCTTCAAGTT TGGATAGTCA GTCGTTTGTG ATGCTGCCCT CAAGTGATGA AGTGGTGACT 
AATCTGAAAA CTCTGACTGG CAAATGCCTC CATGGGAAAA ACTACTGCCG GCAGGTCCTC 
TGTCTGTATG AACTTGCCAA GGAGTTGGGT TGTTCTTACA CAGATGTGGC TGCCCAGGAT 
GGTGAGGCCA TGCTCCGGAC AATCTTGGCC TCTCAGCAGC CTGACCGGTG CAAGCGAGCC 
CAAGCCTTCA TCAGCACCCA GGGCCTCGAG CCAGACACGG TGGCTGAACT TGTGGCCGAG 
GAGGTGACCA GAGAGCTGCT GACCCCATCA GAAAGAGCAG GACAGAAGCA GATGTTCACC 
CCAGCAGAGG AAAGCCAGAC TTTTCTTCCA CTGACCACTT TGTGTGCAGA CCGCACATTG 
GTAGGCATGA AGTTGTTGGA TAAGATTTCC TCCGTTCCCC ATGGGGAGTT GTCTTGCACC 
ACAGAGCTGC TGATCCTGGC CCATCACTGC TTCACGCTGA CATGCCACAT GGAGGGCATC 
ATCCGAGTCC TGCAGGCCGC CCGGCTGCTC ACAGACAACC ACTTGGCCCC CAATGAAGAG 
TACGGGCTGG TGGTGCGTCT CCTCACTGGC ATTGGAAGGT ACAATGAGAT GACATACATA 
TTTGATTTGC TGCATAAAAA GCACTACTTT GAAGTGCTGA TGAGGAAGAA ATTAGATCCG 
AGTGGTACCC TGAAGACAGC CCTACTCGAC TACATCAAAC GCTGCCGCCC TGGGGACAGT 
GAGAAGCACA ACATGATTGC CTTGTGCTTC AGCATGTGCC GTGAGATTGG GGAGAACCAT 
GAGGCAGCTG CCTGCATCCA GCTGAAATTG ATCGAGTCTC AGCCCTGGGA GGACAGCCTC 
AAAGATGGGC ACCAACTGAA GCAGCTGCTG CTGAAGGCCC TGACTCTGAT GCTGGATGCA 
GCAGAGAGTT ATGCCAAGGA CTCTTGCGTG CGACAGGCCC TGCACTGTCA TCGGCTCACC 
AAGTTGATAA CACTGCAGAT TCACTTTCTG AACACTGGCC AGAACACAAT GCTCATCAAC 
CTGAGCCGCC ACAGGCTGAT GGACTGTATC ATGACCCTAC CTCGGTTCTA CCAGGCTTCT 
ATTGTGGCGG AGGCCTATGA CTTTGTTCCT GATTGGGCTG AAATTCTATA CCAACGAGTG 
ATTCTTAAAG GAGACTTTAA TTACTTGGAA GAGTTTAAGC AGCAAAGGTT ATTAAAATCC 
AATATATTTG AAGAGATTTC AAAAAAATAT AAACAACATC AGCCTACCGA CACAGTTGTG 
AAAAACCTGA AGAAGTTACT CACGTATTGT GAAGATGTCT ACTTGTATTA CAAACTAGCT 
TATGAACACA AATTTTGTGA TATTGTAAAT GTGCTTCTGA AGGACCCTCA GATAGGGTGC 
TGTTTAAAGG ACATGCTAGC AGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SPG11 cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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