SMCHD1 cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMCHD1
Entrez Gene ID	100062029
Full Name	structural maintenance of chromosomes flexible hinge domain containing 1
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023647549.1	XP_023503317.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1
XM_014727620.1	XP_014583106.1	LOW QUALITY PROTEIN: structural maintenance of chromosomes flexible hinge domain-containing protein 1

Pathways from BioSystems
Gene Ontology

The following SMCHD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMCHD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHb32431	XM_014727620.1 Latest version!	Equus caballus structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHb70067	XM_023647549.1 Latest version!	Equus caballus structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMCHD1 ( XM_014727620.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014583106.1 Equus caballus structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), mRNA.

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CloneID	OHb32431
Clone ID Related Accession (Same CDS sequence)	XM_014727620.1
Accession Version	XM_014727620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1447862400000
Organism	Equus caballus(horse)
Product	LOW QUALITY PROTEIN: structural maintenance of chromosomes flexible hinge domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867430.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 2 indels internal stop codons :: corrected 1 genomic stop codons ##RefSeq-Attributes-END##

ATGAACCCAC CTATGCTCAT CACTCAGTTT CGACAGTTAG CACGTATTTA CCACACCAGC 
ATCATTTTTG TCTTTTTTAG CACATTCCAG ACACTTGGCA TTTCACCTGA AGAGAAATTT 
GTTATTACAA CAACAAGTAG GAAAGAAGTT ACCTGTGATA ATTTTGATGA GACTGTTAAA 
GATGGAGTCA CTCTATACCT GCTACAGTCA GTCAATCAGT TACTACAAAC AGCTACAAAA 
GAACGAATTG ACTTCCTACC TCACTATGAC ACACTGGTTA AAAGTGGCAT GTATGAATAT 
TATGCAAGTG AAGGACAAAA TCCTTTACCA TTTGCTCTTG CAGAATTGAT TGACAATTCA 
TTGTCTGCTA CTTCTCGTAA CACTGGTATT AGAAGAATAC AGATCAAATT GCATTTTGAT 
GAAACACAAG GAAAACCTGC CGTTGCAGTG ATAGATAATG GAAGAGGAAT GACCTCTAAA 
CAGCTTAACA ACTGGGCCGT GTATAGGTTG TCAAAATTTA CAAGGCAAGG TGACTTTGAA 
AGTGATCATT CGGGATATGT TCGACCAGTA CCAGTGCCAC GCAGTTTAAA CAGTGATATT 
TCCTATTTTG GTGTTGGGGG CAAGCAGGCT GTTTTCTTTG TTGGACAGTC AGCCAGAATG 
ATAAGCAAAC CTGCAGATTC CCAAGATGTT CATGAGCTTG TGCTTTCTAA AGAAGATTTT 
GAGAAGAAGG AGAAAAACAA AGAGGCAATA TATAGTGGAT ATATTCAAAA CAGAAAGCCA 
TCTGATTCTG TTCACATTAC AAATGATGAT GAAAGATTTC TACATCATCT TATCTCAGAG 
GAGAACGAAA AAGATAGCTT TACTGCTGTG GTTATCACAG GAGTACAACC AGAACACATA 
CAATACTTGA AAAATTATTT CCCTCTTTGG ACAAGACAGT TAGCGCATAT TTATCATTAC 
TATATTCATG GTCCAAAAGG AAATGAAAAA CGTACATCAA AAGAAGTTGG ACCTTTCAAC 
AACATTGATA TTGAAATTTC TATGTTTGAA AAGGGGAAGG CACCTAAGAT TGTCAACCTA 
AGGGAAATAC AAGATGACAT GCAGACGTTG TATATAAACA CAGCAGCTGA TAGCTTTGAG 
TTCAAGGCTC ATGTTGAAGG AGACGGTGTA GTGGAAGGGA TTATCCGATA TCACCCTTTC 
TTATATGATA GAGAAACTTA CCCTGATGAT CCATGCTTTC CATCAAAATT AAAAGATGAA 
GATGATGATG ATGATTGTTT CATACTTGAG AAAGCAGCAA GAGGGAAAAG GCCTATTTTT 
GAATGTTTTT GGAATGGACG ACTAATACCA TATACTTCTG TTGAAGACTT TGACTGGTGT 
ACTCCTCCTA AGAAGAGAGG GCTTGCACCA ATTGAGTGCT ACAATAGAAT ATCTGGTGCA 
TTATTCACTA ATGACAAATT CCAGGTCAGC ACAAATAAAT TGACTTTTAT GGATCTTGAG 
CTAAAACTGA AAGATAAGAA CACCCTTTTT ACAAGGATTT TAAATGGACA GGAGCAGCGA 
ATGAAAATTG ACAGAGAATT TGCTCTATGG CTGAAGGACT GTCATGAAAA GTATGACAAA 
CAAATAAAAT TTACACTTTT TAAAGGAATA ATTACACGTC CTGATCTTCC TTCTAAAAAG 
CAGGGCCCCT GGGCGACATA CTCAGCAATA GAATGGGATG GAAAAATATA CAAAGCAGGA 
CAGCTGGTCA AGACAATCAA GACACTGCCT CTCTTTTATG GAAGCATAGT AAAATTTTTT 
CTTTATGGTG ATCATGATGG AGAAGTATAT GCTACAGGAG GAGAGGTTCA AATTGCAATG 
GAACCTCAGG CACTGTATGA TGAAACAAGA ACTGTGCCAA TTGCAAAACT GGATAGGACG 
GTTGCTGAGA AGACTGTAAA AAAATACGTG GAAGATGAAA TGGCAAGGCT CCCTGATAGA 
TTGTCAGTAA CTTGGCCTGA AGGAGATGAA TTATTGCCAA ATGAGGTTAG GCCTGCTGGA 
ACACCCATTG GTGCATTAAG AATTGAAATA CTGAATAAAA AAGGGGAAGC AATGCAGAAG 
CTTCCAGGAA CAAGTCATGG AGGGTCAAAG AAACTCTTGG TTGAGCTCAA AGTTATATTA 
CACTCTTCAA GTGGAAATAA AGAAATAATT TCACATATTA GTCAGCATGG AGGAAAATGG 
CCTTACTGGT TTAAAAAAAT GGAAAATATT CAAAAGTTGG GGAATTATAC ACTGAAATTA 
CAAGTTGTAT TGAATGAAAG TAATGCAGAC ACTTATGCAG GAAGACCATT ACCATCTAAA 
GCAATTAAAT TTTCTGTTAA AGAGGGTAAG CCAGAGAAAT TTTCATTTGG TCTTCTGGAT 
CCTCCTTTTC GTGTTGGAGT CCCATTCAAT ATTCCTCTGG AGTTTCAAGA TGAATTTGGT 
CATACTAGTC AACTAATAAA TGATATTCAG CCAGTTCTTG AAGCAAGTGG TTTATCTTTA 
CATTATGAAG AAATAACCAA AGGACCGAAT TGTGTAATCC GAGGTGTTAC AGCTAAGGGC 
CCTGTAAACT CTTGTCAAGG CAAGAATTTT AATCTGAAGG TTATTCTGCC GGGTTTAAAA 
GAAGACTCAC AGATTTTGAA AATTAGATTA CTACCAGGTC CCCCTCGTCG ATTGAAAGTG 
AAACCTGATT CTGAAATTTT AGTTATAGAA AATGGAACAG CTTTCCCATT TCAGGTAGAA 
GTTTTGGATG AATCAGACAA TGTAACAGCA CAACCAAAAC TGATTGTTCA TTGTAAGTTT 
TTAGGTGCTC CAAACCTTCC AGTTTATGTT GTGGATTGCA GTAGTTCTGG AACCAGTATT 
TTATCAGGAT CTGCAATTCA AGTTCAGAAT ATTAAAAAAG ACCAGACGCT TAAGGCTAAA 
ATTGAAATAC CTAGTTGTAA AGATGTAGCC CCTGTGGAGA AGACTATTAA GTTGCTTCCC 
AGTAGCCATG TTGCAAGATT ACAGATTTTC AGTGTGGAAG GACAAAAGGC AATCCAGATA 
AAACATCAGG ATGAGGTCAA TTGGATAGCA GGCGATATTA TGCACAACCT TATTTTTCAA 
ATGTATGATG AAGGAGAAAG AGAAATCCAT ATAACATCAG CTTTAGCAGA GAAAATTAAA 
GTTAATTGGA CTCCTGAGAT TAACAAAGAA CACTTGCTGC AGGGTCTGCT TCCTGATGTA 
CAAGTACCAA CGTCTGTAAA AGATATGCGT TATTGCCAGG TTTCATTCCA AGATGATCAT 
GTGTCTTTGG AAAGTGCATT TACAGTAAGA CCACTTCCTG ATGAACCTAA ACATCTAAAA 
TGTGAGTTGA AAGGAGGAAA AACAGTACAG ATGGGCCAAG AGCTTCAAGG AGAAATAGTT 
ATAATAGTTA CAGATCAATA TGGAAATCAG ATTCAAGCAT TTTCACCAAG TTCTTTATCT 
GCCTTGGGAA TTGCTGGGAT TGGACTTGAT AGCTCACGTT TGAAAACTAC TTTTCAGGAA 
AATACACAAA GTATAAGTGT AAGAGGCGTC AAATTTTTTC CAGGTCCTCC TGGAAATAAG 
GATCTTTGTT TTACTTGGCG TGAGTTTTCT GACTTATTCG AGTGCAGCTA AATTTCTGGA 
CCACCAGCTA AACTTCTCCT CATAGACTGG CCCAGAATTA AAGAGTCCAT TCCGGTGATT 
AATGGAAGAG AATTACAGCA TCCTCTCATT GTTCAACTTT GTGATCAGTG GGATAATCCA 
GCACCCGTAC CACAAGTTAA AATAAGTCTC ATAAAAGCTA ACTACTTAAA ACTCACCCCT 
TCAAACCAAC AGCATAAAAC AGATGAAAGG GGCAAGGCTA ATTTGGGAGT GTTCAGTGTT 
TATGCCCCTA GAGGAGAGCA TACTATTCAG ATTAAAGCCG TCTATAACAA GACTACCATA 
GAAGGACCTA TAATTAAGTT AATGATTCTT CCAGACCCTG AAAAACCCAT TCGTCTCAAT 
GTTAAATATG ACAAAGATGC TTCCTTTTTA GCAGGAGGTG TTTTCACTGA TTTTATGATT 
ACTGTTATTT CTGAAGATGA CAGTATCATT AAAAATATTA ATCCAGCACG TATTACTATG 
AAAATGTGGA AGCTGTCTAA CAGTGGGAGC CGACCACCAG CAAATGCAGA AACATTCAGT 
TGTAATAAAA TAAAAGATAA TGACAAGGAA GATGGCTGCT TCTACTTCAG GGATAAAGCA 
ATTCCTAACA AAGTGGGGAC ATATTGTATC CAGTTTGGTT TTATGATGGA TAAAGCAAAT 
ATTCTTAACA GTGAACAGGT TATAGTTGAT GTTCTGCCTA ATCAACCTGT GAAGTTAGTT 
CCGGAAATTC AGCCAGCTAC ACCAGCTGTT TCAAATGTTC GCTCAGTTGC CAGTAGGACC 
TTAGTCAAAG ATTTACATCT TAATATCACG GATGACTACA ACAATTATAC CGGAATTGAT 
TTGGTTGGCA CTGTAATAGC TACCATCAAA GGTTCTAATG AGGAAGATAA TGATACACCT 
CTCTTTATTG GAAAAGTTAG AACACTTGAA TTTCCCTTTG TGAAAGGTTC AGCTGAAATC 
ACGAGCCTAG TGCTGGCAGA AAATAGTCCT GGAAGGGATA GTACCGAATA TTTTATTATA 
TTTGAGCCTC GGCTACCAGC TTTATCAAGA AAATTAGAAT CATATATCCT ACCGTTTATG 
TTTTACAATG ATGTTAAGAA GCAGCAACAA ATGGCAGAAC TTACTAAAGA AAAGGACCAA 
TTATCTAAAT CTATTATAAT GTATAGAAGT TTATTTGAAG CCAGCAAACA GCTTCTTGAT 
GAAATGAAGT GCCAAGTTGA AGAAGCAAAA TTAAAAGAGG CCCAGTTGAG AAATGAACTA 
AAAGCACATA ATATTGACAT TCCTACAACA CAACAGATGC CACACATTGA AGCGCTTTTG 
AAAAGAAAGA CATCAGAACA GGAAGAACTA AGGAAAAAAC CTAGAAGATC ATGTACTCTT 
CCAAACTACA CTAAAGGCAG TGGAGATGTT TTGGGAAAGA TTGCACACCT GGCACAAATT 
GAAGATGATA GAGCTGCCAT GGTTATTTCT TGGCATCTAG CAAGTGACAT GGACTGTGTG 
GTCACCCTGA CCACTGATGC TGCACGTCGT ATCTATGATG AAACACAAGG TCGTCAGCAG 
GTGTTGCCCC TTGATTCTAT TTATAAGAAG ACTCTTCCAG ATTGGAAAAG ACCTCTACCT 
CATTACCGAA ATGGAAAATT ATATTTTAAA CCCGTTGGAG ATCCAGTCTT TGCCCGAGAT 
TTGTTAACAT TTCCAGATAA CGTAGAACAT TGTGAAACAG TATTTGGTAT GCTGTTAGGA 
GACACCATTA TTTTGGATAA TTTGGATGCT GCCAATCATT ATAGAAAAGA GGTTGTTAAA 
ATTACACACT GTCCTACACT GCTGACCAGA GATGGAGATC GCATTCGAAG TAATGGAAAG 
TTTGGCGGCC TTCAAAATAA AGCTCCTCCA ATGGATAAAC TTCGGGGAAT GGTATTTGGA 
GCTCCAATCC CAAAACAGTG CCTGATCTTA GGGGAACAAA TAGATCTTCT TCAGCAGTAT 
CGTACTGCTG TGTGCAAGTT GGGTGCTGTG AATGATGACC TTAATGGTCA GTTAGAGTAC 
CTTCACACGC CTGATATGAA GAAGAAAAAA CAAGAACTCG ATGAACAAGA GAAAAACCTG 
AAACTAATAG AGCAAAAACT AGGTATGACT CCCACACGTA AGTGTAGTGA TTCATTGCGT 
CATCCAATGG CTGATTGTCC AATTCCTCCT AAAAGAATGA GAAGAGACAC TACAAGACAA 
AATAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014583106.1
CDS	1..5889
Translation	MNPPMLITQFRQLARIYHTSIIFVFFSTFQTLGISPEEKFVITTTSRKEVTCDNFDETVKDGVTLYLLQSVNQLLQTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNTGIRRIQIKLHFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIQNRKPSDSVHITNDDERFLHHLISEENEKDSFTAVVITGVQPEHIQYLKNYFPLWTRQLAHIYHYYIHGPKGNEKRTSKEVGPFNNIDIEISMFEKGKAPKIVNLREIQDDMQTLYINTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDDDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTLFKGIITRPDLPSKKQGPWATYSAIEWDGKIYKAGQLVKTIKTLPLFYGSIVKFFLYGDHDGEVYATGGEVQIAMEPQALYDETRTVPIAKLDRTVAEKTVKKYVEDEMARLPDRLSVTWPEGDELLPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLLDPPFRVGVPFNIPLEFQDEFGHTSQLINDIQPVLEASGLSLHYEEITKGPNCVIRGVTAKGPVNSCQGKNFNLKVILPGLKEDSQILKIRLLPGPPRRLKVKPDSEILVIENGTAFPFQVEVLDESDNVTAQPKLIVHCKFLGAPNLPVYVVDCSSSGTSILSGSAIQVQNIKKDQTLKAKIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREIHITSALAEKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCELKGGKTVQMGQELQGEIVIIVTDQYGNQIQAFSPSSLSALGIAGIGLDSSRLKTTFQENTQSISVRGVKFFPGPPGNKDLCFTWREFSDLFECS*ISGPPAKLLLIDWPRIKESIPVINGRELQHPLIVQLCDQWDNPAPVPQVKISLIKANYLKLTPSNQQHKTDERGKANLGVFSVYAPRGEHTIQIKAVYNKTTIEGPIIKLMILPDPEKPIRLNVKYDKDASFLAGGVFTDFMITVISEDDSIIKNINPARITMKMWKLSNSGSRPPANAETFSCNKIKDNDKEDGCFYFRDKAIPNKVGTYCIQFGFMMDKANILNSEQVIVDVLPNQPVKLVPEIQPATPAVSNVRSVASRTLVKDLHLNITDDYNNYTGIDLVGTVIATIKGSNEEDNDTPLFIGKVRTLEFPFVKGSAEITSLVLAENSPGRDSTEYFIIFEPRLPALSRKLESYILPFMFYNDVKKQQQMAELTKEKDQLSKSIIMYRSLFEASKQLLDEMKCQVEEAKLKEAQLRNELKAHNIDIPTTQQMPHIEALLKRKTSEQEELRKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRPLPHYRNGKLYFKPVGDPVFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPIPKQCLILGEQIDLLQQYRTAVCKLGAVNDDLNGQLEYLHTPDMKKKKQELDEQEKNLKLIEQKLGMTPTRKCSDSLRHPMADCPIPPKRMRRDTTRQNR

Target ORF information:

RefSeq Version	XM_014727620.1
Organism	Equus caballus(horse)
Definition	Equus caballus structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014727620.1

ORF Insert Sequence:

ATGAACCCAC CTATGCTCAT CACTCAGTTT CGACAGTTAG CACGTATTTA CCACACCAGC 
ATCATTTTTG TCTTTTTTAG CACATTCCAG ACACTTGGCA TTTCACCTGA AGAGAAATTT 
GTTATTACAA CAACAAGTAG GAAAGAAGTT ACCTGTGATA ATTTTGATGA GACTGTTAAA 
GATGGAGTCA CTCTATACCT GCTACAGTCA GTCAATCAGT TACTACAAAC AGCTACAAAA 
GAACGAATTG ACTTCCTACC TCACTATGAC ACACTGGTTA AAAGTGGCAT GTATGAATAT 
TATGCAAGTG AAGGACAAAA TCCTTTACCA TTTGCTCTTG CAGAATTGAT TGACAATTCA 
TTGTCTGCTA CTTCTCGTAA CACTGGTATT AGAAGAATAC AGATCAAATT GCATTTTGAT 
GAAACACAAG GAAAACCTGC CGTTGCAGTG ATAGATAATG GAAGAGGAAT GACCTCTAAA 
CAGCTTAACA ACTGGGCCGT GTATAGGTTG TCAAAATTTA CAAGGCAAGG TGACTTTGAA 
AGTGATCATT CGGGATATGT TCGACCAGTA CCAGTGCCAC GCAGTTTAAA CAGTGATATT 
TCCTATTTTG GTGTTGGGGG CAAGCAGGCT GTTTTCTTTG TTGGACAGTC AGCCAGAATG 
ATAAGCAAAC CTGCAGATTC CCAAGATGTT CATGAGCTTG TGCTTTCTAA AGAAGATTTT 
GAGAAGAAGG AGAAAAACAA AGAGGCAATA TATAGTGGAT ATATTCAAAA CAGAAAGCCA 
TCTGATTCTG TTCACATTAC AAATGATGAT GAAAGATTTC TACATCATCT TATCTCAGAG 
GAGAACGAAA AAGATAGCTT TACTGCTGTG GTTATCACAG GAGTACAACC AGAACACATA 
CAATACTTGA AAAATTATTT CCCTCTTTGG ACAAGACAGT TAGCGCATAT TTATCATTAC 
TATATTCATG GTCCAAAAGG AAATGAAAAA CGTACATCAA AAGAAGTTGG ACCTTTCAAC 
AACATTGATA TTGAAATTTC TATGTTTGAA AAGGGGAAGG CACCTAAGAT TGTCAACCTA 
AGGGAAATAC AAGATGACAT GCAGACGTTG TATATAAACA CAGCAGCTGA TAGCTTTGAG 
TTCAAGGCTC ATGTTGAAGG AGACGGTGTA GTGGAAGGGA TTATCCGATA TCACCCTTTC 
TTATATGATA GAGAAACTTA CCCTGATGAT CCATGCTTTC CATCAAAATT AAAAGATGAA 
GATGATGATG ATGATTGTTT CATACTTGAG AAAGCAGCAA GAGGGAAAAG GCCTATTTTT 
GAATGTTTTT GGAATGGACG ACTAATACCA TATACTTCTG TTGAAGACTT TGACTGGTGT 
ACTCCTCCTA AGAAGAGAGG GCTTGCACCA ATTGAGTGCT ACAATAGAAT ATCTGGTGCA 
TTATTCACTA ATGACAAATT CCAGGTCAGC ACAAATAAAT TGACTTTTAT GGATCTTGAG 
CTAAAACTGA AAGATAAGAA CACCCTTTTT ACAAGGATTT TAAATGGACA GGAGCAGCGA 
ATGAAAATTG ACAGAGAATT TGCTCTATGG CTGAAGGACT GTCATGAAAA GTATGACAAA 
CAAATAAAAT TTACACTTTT TAAAGGAATA ATTACACGTC CTGATCTTCC TTCTAAAAAG 
CAGGGCCCCT GGGCGACATA CTCAGCAATA GAATGGGATG GAAAAATATA CAAAGCAGGA 
CAGCTGGTCA AGACAATCAA GACACTGCCT CTCTTTTATG GAAGCATAGT AAAATTTTTT 
CTTTATGGTG ATCATGATGG AGAAGTATAT GCTACAGGAG GAGAGGTTCA AATTGCAATG 
GAACCTCAGG CACTGTATGA TGAAACAAGA ACTGTGCCAA TTGCAAAACT GGATAGGACG 
GTTGCTGAGA AGACTGTAAA AAAATACGTG GAAGATGAAA TGGCAAGGCT CCCTGATAGA 
TTGTCAGTAA CTTGGCCTGA AGGAGATGAA TTATTGCCAA ATGAGGTTAG GCCTGCTGGA 
ACACCCATTG GTGCATTAAG AATTGAAATA CTGAATAAAA AAGGGGAAGC AATGCAGAAG 
CTTCCAGGAA CAAGTCATGG AGGGTCAAAG AAACTCTTGG TTGAGCTCAA AGTTATATTA 
CACTCTTCAA GTGGAAATAA AGAAATAATT TCACATATTA GTCAGCATGG AGGAAAATGG 
CCTTACTGGT TTAAAAAAAT GGAAAATATT CAAAAGTTGG GGAATTATAC ACTGAAATTA 
CAAGTTGTAT TGAATGAAAG TAATGCAGAC ACTTATGCAG GAAGACCATT ACCATCTAAA 
GCAATTAAAT TTTCTGTTAA AGAGGGTAAG CCAGAGAAAT TTTCATTTGG TCTTCTGGAT 
CCTCCTTTTC GTGTTGGAGT CCCATTCAAT ATTCCTCTGG AGTTTCAAGA TGAATTTGGT 
CATACTAGTC AACTAATAAA TGATATTCAG CCAGTTCTTG AAGCAAGTGG TTTATCTTTA 
CATTATGAAG AAATAACCAA AGGACCGAAT TGTGTAATCC GAGGTGTTAC AGCTAAGGGC 
CCTGTAAACT CTTGTCAAGG CAAGAATTTT AATCTGAAGG TTATTCTGCC GGGTTTAAAA 
GAAGACTCAC AGATTTTGAA AATTAGATTA CTACCAGGTC CCCCTCGTCG ATTGAAAGTG 
AAACCTGATT CTGAAATTTT AGTTATAGAA AATGGAACAG CTTTCCCATT TCAGGTAGAA 
GTTTTGGATG AATCAGACAA TGTAACAGCA CAACCAAAAC TGATTGTTCA TTGTAAGTTT 
TTAGGTGCTC CAAACCTTCC AGTTTATGTT GTGGATTGCA GTAGTTCTGG AACCAGTATT 
TTATCAGGAT CTGCAATTCA AGTTCAGAAT ATTAAAAAAG ACCAGACGCT TAAGGCTAAA 
ATTGAAATAC CTAGTTGTAA AGATGTAGCC CCTGTGGAGA AGACTATTAA GTTGCTTCCC 
AGTAGCCATG TTGCAAGATT ACAGATTTTC AGTGTGGAAG GACAAAAGGC AATCCAGATA 
AAACATCAGG ATGAGGTCAA TTGGATAGCA GGCGATATTA TGCACAACCT TATTTTTCAA 
ATGTATGATG AAGGAGAAAG AGAAATCCAT ATAACATCAG CTTTAGCAGA GAAAATTAAA 
GTTAATTGGA CTCCTGAGAT TAACAAAGAA CACTTGCTGC AGGGTCTGCT TCCTGATGTA 
CAAGTACCAA CGTCTGTAAA AGATATGCGT TATTGCCAGG TTTCATTCCA AGATGATCAT 
GTGTCTTTGG AAAGTGCATT TACAGTAAGA CCACTTCCTG ATGAACCTAA ACATCTAAAA 
TGTGAGTTGA AAGGAGGAAA AACAGTACAG ATGGGCCAAG AGCTTCAAGG AGAAATAGTT 
ATAATAGTTA CAGATCAATA TGGAAATCAG ATTCAAGCAT TTTCACCAAG TTCTTTATCT 
GCCTTGGGAA TTGCTGGGAT TGGACTTGAT AGCTCACGTT TGAAAACTAC TTTTCAGGAA 
AATACACAAA GTATAAGTGT AAGAGGCGTC AAATTTTTTC CAGGTCCTCC TGGAAATAAG 
GATCTTTGTT TTACTTGGCG TGAGTTTTCT GACTTATTCG AGTGCAGCTA AATTTCTGGA 
CCACCAGCTA AACTTCTCCT CATAGACTGG CCCAGAATTA AAGAGTCCAT TCCGGTGATT 
AATGGAAGAG AATTACAGCA TCCTCTCATT GTTCAACTTT GTGATCAGTG GGATAATCCA 
GCACCCGTAC CACAAGTTAA AATAAGTCTC ATAAAAGCTA ACTACTTAAA ACTCACCCCT 
TCAAACCAAC AGCATAAAAC AGATGAAAGG GGCAAGGCTA ATTTGGGAGT GTTCAGTGTT 
TATGCCCCTA GAGGAGAGCA TACTATTCAG ATTAAAGCCG TCTATAACAA GACTACCATA 
GAAGGACCTA TAATTAAGTT AATGATTCTT CCAGACCCTG AAAAACCCAT TCGTCTCAAT 
GTTAAATATG ACAAAGATGC TTCCTTTTTA GCAGGAGGTG TTTTCACTGA TTTTATGATT 
ACTGTTATTT CTGAAGATGA CAGTATCATT AAAAATATTA ATCCAGCACG TATTACTATG 
AAAATGTGGA AGCTGTCTAA CAGTGGGAGC CGACCACCAG CAAATGCAGA AACATTCAGT 
TGTAATAAAA TAAAAGATAA TGACAAGGAA GATGGCTGCT TCTACTTCAG GGATAAAGCA 
ATTCCTAACA AAGTGGGGAC ATATTGTATC CAGTTTGGTT TTATGATGGA TAAAGCAAAT 
ATTCTTAACA GTGAACAGGT TATAGTTGAT GTTCTGCCTA ATCAACCTGT GAAGTTAGTT 
CCGGAAATTC AGCCAGCTAC ACCAGCTGTT TCAAATGTTC GCTCAGTTGC CAGTAGGACC 
TTAGTCAAAG ATTTACATCT TAATATCACG GATGACTACA ACAATTATAC CGGAATTGAT 
TTGGTTGGCA CTGTAATAGC TACCATCAAA GGTTCTAATG AGGAAGATAA TGATACACCT 
CTCTTTATTG GAAAAGTTAG AACACTTGAA TTTCCCTTTG TGAAAGGTTC AGCTGAAATC 
ACGAGCCTAG TGCTGGCAGA AAATAGTCCT GGAAGGGATA GTACCGAATA TTTTATTATA 
TTTGAGCCTC GGCTACCAGC TTTATCAAGA AAATTAGAAT CATATATCCT ACCGTTTATG 
TTTTACAATG ATGTTAAGAA GCAGCAACAA ATGGCAGAAC TTACTAAAGA AAAGGACCAA 
TTATCTAAAT CTATTATAAT GTATAGAAGT TTATTTGAAG CCAGCAAACA GCTTCTTGAT 
GAAATGAAGT GCCAAGTTGA AGAAGCAAAA TTAAAAGAGG CCCAGTTGAG AAATGAACTA 
AAAGCACATA ATATTGACAT TCCTACAACA CAACAGATGC CACACATTGA AGCGCTTTTG 
AAAAGAAAGA CATCAGAACA GGAAGAACTA AGGAAAAAAC CTAGAAGATC ATGTACTCTT 
CCAAACTACA CTAAAGGCAG TGGAGATGTT TTGGGAAAGA TTGCACACCT GGCACAAATT 
GAAGATGATA GAGCTGCCAT GGTTATTTCT TGGCATCTAG CAAGTGACAT GGACTGTGTG 
GTCACCCTGA CCACTGATGC TGCACGTCGT ATCTATGATG AAACACAAGG TCGTCAGCAG 
GTGTTGCCCC TTGATTCTAT TTATAAGAAG ACTCTTCCAG ATTGGAAAAG ACCTCTACCT 
CATTACCGAA ATGGAAAATT ATATTTTAAA CCCGTTGGAG ATCCAGTCTT TGCCCGAGAT 
TTGTTAACAT TTCCAGATAA CGTAGAACAT TGTGAAACAG TATTTGGTAT GCTGTTAGGA 
GACACCATTA TTTTGGATAA TTTGGATGCT GCCAATCATT ATAGAAAAGA GGTTGTTAAA 
ATTACACACT GTCCTACACT GCTGACCAGA GATGGAGATC GCATTCGAAG TAATGGAAAG 
TTTGGCGGCC TTCAAAATAA AGCTCCTCCA ATGGATAAAC TTCGGGGAAT GGTATTTGGA 
GCTCCAATCC CAAAACAGTG CCTGATCTTA GGGGAACAAA TAGATCTTCT TCAGCAGTAT 
CGTACTGCTG TGTGCAAGTT GGGTGCTGTG AATGATGACC TTAATGGTCA GTTAGAGTAC 
CTTCACACGC CTGATATGAA GAAGAAAAAA CAAGAACTCG ATGAACAAGA GAAAAACCTG 
AAACTAATAG AGCAAAAACT AGGTATGACT CCCACACGTA AGTGTAGTGA TTCATTGCGT 
CATCCAATGG CTGATTGTCC AATTCCTCCT AAAAGAATGA GAAGAGACAC TACAAGACAA 
AATAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCHD1 ( XM_023647549.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023503317.1 Equus caballus structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), mRNA.

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CloneID	OHb70067
Clone ID Related Accession (Same CDS sequence)	XM_023647549.1
Accession Version	XM_023647549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009151.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG CGGGCGGCGG CGGGCCCTCG GTGGGGACTG GAGAGGGCGG CGGCGGCGAC 
GGCTACAGGA CAGTGTACTT GTTTGACCGG CGCGAGAAGG AGTCCGAGCT CGGGGACCGG 
GTGCTGAAGG TCGGGGAGGG CTCGGACTAC GCGGGGTTCC GCGCCTCCGT GTGTCAGACA 
CTTGGCATTT CACCTGAAGA GAAATTTGTT ATTACAACAA CAAGTAGGAA AGAAGTTACC 
TGTGATAATT TTGATGAGAC TGTTAAAGAT GGAGTCACTC TATACCTGCT ACAGTCAGTC 
AATCAGTTAC TACAAACAGC TACAAAAGAA CGAATTGACT TCCTACCTCA CTATGACACA 
CTGGTTAAAA GTGGCATGTA TGAATATTAT GCAAGTGAAG GACAAAATCC TTTACCATTT 
GCTCTTGCAG AATTGATTGA CAATTCATTG TCTGCTACTT CTCGTAACAC TGGTATTAGA 
AGAATACAGA TCAAATTGCA TTTTGATGAA ACACAAGGAA AACCTGCCGT TGCAGTGATA 
GATAATGGAA GAGGAATGAC CTCTAAACAG CTTAACAACT GGGCCGTGTA TAGGTTGTCA 
AAATTTACAA GGCAAGGTGA CTTTGAAAGT GATCATTCGG GATATGTTCG ACCAGTACCA 
GTGCCACGCA GTTTAAACAG TGATATTTCC TATTTTGGTG TTGGGGGCAA GCAGGCTGTT 
TTCTTTGTTG GACAGTCAGC CAGAATGATA AGCAAACCTG CAGATTCCCA AGATGTTCAT 
GAGCTTGTGC TTTCTAAAGA AGATTTTGAG AAGAAGGAGA AAAACAAAGA GGCAATATAT 
AGTGGATATA TTCAAAACAG AAAGCCATCT GATTCTGTTC ACATTACAAA TGATGATGAA 
AGATTTCTAC ATCATCTTAT CTCAGAGGAG AACGAAAAAG ATAGCTTTAC TGCTGTGGTT 
ATCACAGGAG TACAACCAGA ACACATACAA TACTTGAAAA ATTATTTCCC TCTTTGGACA 
AGACAGTTAG CGCATATTTA TCATTACTAT ATTCATGGTC CAAAAGGAAA TGAAAAACGT 
ACATCAAAAG AAGTTGGACC TTTCAACAAC ATTGATATTG AAATTTCTAT GTTTGAAAAG 
GGGAAGGCAC CTAAGATTGT CAACCTAAGG GAAATACAAG ATGACATGCA GACGTTGTAT 
ATAAACACAG CAGCTGATAG CTTTGAGTTC AAGGCTCATG TTGAAGGAGA CGGTGTAGTG 
GAAGGGATTA TCCGATATCA CCCTTTCTTA TATGATAGAG AAACTTACCC TGATGATCCA 
TGCTTTCCAT CAAAATTAAA AGATGAAGAT GATGATGATG ATTGTTTCAT ACTTGAGAAA 
GCAGCAAGAG GGAAAAGGCC TATTTTTGAA TGTTTTTGGA ATGGACGACT AATACCATAT 
ACTTCTGTTG AAGACTTTGA CTGGTGTACT CCTCCTAAGA AGAGAGGGCT TGCACCAATT 
GAGTGCTACA ATAGAATATC TGGTGCATTA TTCACTAATG ACAAATTCCA GGTCAGCACA 
AATAAATTGA CTTTTATGGA TCTTGAGCTA AAACTGAAAG ATAAGAACAC CCTTTTTACA 
AGGATTTTAA ATGGACAGGA GCAGCGAATG AAAATTGACA GAGAATTTGC TCTATGGCTG 
AAGGACTGTC ATGAAAAGTA TGACAAACAA ATAAAATTTA CACTTTTTAA AGGAATAATT 
ACACGTCCTG ATCTTCCTTC TAAAAAGCAG GGCCCCTGGG CGACATACTC AGCAATAGAA 
TGGGATGGAA AAATATACAA AGCAGGACAG CTGGTCAAGA CAATCAAGAC ACTGCCTCTC 
TTTTATGGAA GCATAGTAAA ATTTTTTCTT TATGGTGATC ATGATGGAGA AGTATATGCT 
ACAGGAGGAG AGGTTCAAAT TGCAATGGAA CCTCAGGCAC TGTATGATGA AACAAGAACT 
GTGCCAATTG CAAAACTGGA TAGGACGGTT GCTGAGAAGA CTGTAAAAAA ATACGTGGAA 
GATGAAATGG CAAGGCTCCC TGATAGATTG TCAGTAACTT GGCCTGAAGG AGATGAATTA 
TTGCCAAATG AGGTTAGGCC TGCTGGAACA CCCATTGGTG CATTAAGAAT TGAAATACTG 
AATAAAAAAG GGGAAGCAAT GCAGAAGCTT CCAGGAACAA GTCATGGAGG GTCAAAGAAA 
CTCTTGGTTG AGCTCAAAGT TATATTACAC TCTTCAAGTG GAAATAAAGA AATAATTTCA 
CATATTAGTC AGCATGGAGG AAAATGGCCT TACTGGTTTA AAAAAATGGA AAATATTCAA 
AAGTTGGGGA ATTATACACT GAAATTACAA GTTGTATTGA ATGAAAGTAA TGCAGACACT 
TATGCAGGAA GACCATTACC ATCTAAAGCA ATTAAATTTT CTGTTAAAGA GGGTAAGCCA 
GAGAAATTTT CATTTGGTCT TCTGGATCCT CCTTTTCGTG TTGGAGTCCC ATTCAATATT 
CCTCTGGAGT TTCAAGATGA ATTTGGTCAT ACTAGTCAAC TAATAAATGA TATTCAGCCA 
GTTCTTGAAG CAAGTGGTTT ATCTTTACAT TATGAAGAAA TAACCAAAGG ACCGAATTGT 
GTAATCCGAG GTGTTACAGC TAAGGGCCCT GTAAACTCTT GTCAAGGCAA GAATTTTAAT 
CTGAAGGTTA TTCTGCCGGG TTTAAAAGAA GACTCACAGA TTTTGAAAAT TAGATTACTA 
CCAGGTCCCC CTCGTCGATT GAAAGTGAAA CCTGATTCTG AAATTTTAGT TATAGAAAAT 
GGAACAGCTT TCCCATTTCA GGTAGAAGTT TTGGATGAAT CAGACAATGT AACAGCACAA 
CCAAAACTGA TTGTTCATTG TAAGTTTTTA GGTGCTCCAA ACCTTCCAGT TTATGTTGTG 
GATTGCAGTA GTTCTGGAAC CAGTATTTTA TCAGGATCTG CAATTCAAGT TCAGAATATT 
AAAAAAGACC AGACGCTTAA GGCTAAAATT GAAATACCTA GTTGTAAAGA TGTAGCCCCT 
GTGGAGAAGA CTATTAAGTT GCTTCCCAGT AGCCATGTTG CAAGATTACA GATTTTCAGT 
GTGGAAGGAC AAAAGGCAAT CCAGATAAAA CATCAGGATG AGGTCAATTG GATAGCAGGC 
GATATTATGC ACAACCTTAT TTTTCAAATG TATGATGAAG GAGAAAGAGA AATCCATATA 
ACATCAGCTT TAGCAGAGAA AATTAAAGTT AATTGGACTC CTGAGATTAA CAAAGAACAC 
TTGCTGCAGG GTCTGCTTCC TGATGTACAA GTACCAACGT CTGTAAAAGA TATGCGTTAT 
TGCCAGGTTT CATTCCAAGA TGATCATGTG TCTTTGGAAA GTGCATTTAC AGTAAGACCA 
CTTCCTGATG AACCTAAACA TCTAAAATGT GAGTTGAAAG GAGGAAAAAC AGTACAGATG 
GGCCAAGAGC TTCAAGGAGA AATAGTTATA ATAGTTACAG ATCAATATGG AAATCAGATT 
CAAGCATTTT CACCAAGTTC TTTATCTGCC TTGGGAATTG CTGGGATTGG ACTTGATAGC 
TCACGTTTGA AAACTACTTT TCAGGAAAAT ACACAAAGTA TAAGTGTAAG AGGCGTCAAA 
TTTATTCCAG GTCCTCCTGG AAATAAGGAT CTTTGTTTTA CTTGGCGTGA GTTTTCTGAC 
TTTATTCGAG TGCAGCTAAT TTCTGGACCA CCAGCTAAAC TTCTCCTCAT AGACTGGCCA 
GAATTAAAGG AGTCCATTCC GGTGATTAAT GGAAGAGAAT TACAGCATCC TCTCATTGTT 
CAACTTTGTG ATCAGTGGGA TAATCCAGCA CCCGTACCAC AAGTTAAAAT AAGTCTCATA 
AAAGCTAACT ACTTAAAACT CACCCCTTCA AACCAACAGC ATAAAACAGA TGAAAGGGGC 
AAGGCTAATT TGGGAGTGTT CAGTGTTTAT GCCCCTAGAG GAGAGCATAC TATTCAGATT 
AAAGCCGTCT ATAACAAGAC TACCATAGAA GGACCTATAA TTAAGTTAAT GATTCTTCCA 
GACCCTGAAA AACCCATTCG TCTCAATGTT AAATATGACA AAGATGCTTC CTTTTTAGCA 
GGAGGTGTTT TCACTGATTT TATGATTACT GTTATTTCTG AAGATGACAG TATCATTAAA 
AATATTAATC CAGCACGTAT TACTATGAAA ATGTGGAAGC TGTCTAACAG TGGGAGCCGA 
CCACCAGCAA ATGCAGAAAC ATTCAGTTGT AATAAAATAA AAGATAATGA CAAGGAAGAT 
GGCTGCTTCT ACTTCAGGGA TAAAGCAATT CCTAACAAAG TGGGGACATA TTGTATCCAG 
TTTGGTTTTA TGATGGATAA AGCAAATATT CTTAACAGTG AACAGGTTAT AGTTGATGTT 
CTGCCTAATC AACCTGTGAA GTTAGTTCCG GAAATTCAGC CAGCTACACC AGCTGTTTCA 
AATGTTCGCT CAGTTGCCAG TAGGACCTTA GTCAAAGATT TACATCTTAA TATCACGGAT 
GACTACAACA ATTATACCGG AATTGATTTG GTTGGCACTG TAATAGCTAC CATCAAAGGT 
TCTAATGAGG AAGATAATGA TACACCTCTC TTTATTGGAA AAGTTAGAAC ACTTGAATTT 
CCCTTTGTGA AAGGTTCAGC TGAAATCACG AGCCTAGTGC TGGCAGAAAA TAGTCCTGGA 
AGGGATAGTA CCGAATATTT TATTATATTT GAGCCTCGGC TACCAGCTTT ATCAAGAAAA 
TTAGAATCAT ATATCCTACC GTTTATGTTT TACAATGATG TTAAGAAGCA GCAACAAATG 
GCAGAACTTA CTAAAGAAAA GGACCAATTA TCTAAATCTA TTATAATGTA TAGAAGTTTA 
TTTGAAGCCA GCAAACAGCT TCTTGATGAA ATGAAGTGCC AAGTTGAAGA AGCAAAATTA 
AAAGAGGCCC AGTTGAGAAA TGAACTAAAA GCACATAATA TTGACATTCC TACAACACAA 
CAGATGCCAC ACATTGAAGC GCTTTTGAAA AGAAAGACAT CAGAACAGGA AGAACTAAGG 
AAAAAACCTA GAAGATCATG TACTCTTCCA AACTACACTA AAGGCAGTGG AGATGTTTTG 
GGAAAGATTG CACACCTGGC ACAAATTGAA GATGATAGAG CTGCCATGGT TATTTCTTGG 
CATCTAGCAA GTGACATGGA CTGTGTGGTC ACCCTGACCA CTGATGCTGC ACGTCGTATC 
TATGATGAAA CACAAGGTCG TCAGCAGGTG TTGCCCCTTG ATTCTATTTA TAAGAAGACT 
CTTCCAGATT GGAAAAGACC TCTACCTCAT TACCGAAATG GAAAATTATA TTTTAAACCC 
GTTGGAGATC CAGTCTTTGC CCGAGATTTG TTAACATTTC CAGATAACGT AGAACATTGT 
GAAACAGTAT TTGGTATGCT GTTAGGAGAC ACCATTATTT TGGATAATTT GGATGCTGCC 
AATCATTATA GAAAAGAGGT TGTTAAAATT ACACACTGTC CTACACTGCT GACCAGAGAT 
GGAGATCGCA TTCGAAGTAA TGGAAAGTTT GGCGGCCTTC AAAATAAAGC TCCTCCAATG 
GATAAACTTC GGGGAATGGT ATTTGGAGCT CCAATCCCAA AACAGTGCCT GATCTTAGGG 
GAACAAATAG ATCTTCTTCA GCAGTATCGT ACTGCTGTGT GCAAGTTGGG TGCTGTGAAT 
GATGACCTTA ATGGTCAGTT AGAGTACCTT CACACGCCTG ATATGAAGAA GAAAAAACAA 
GAACTCGATG AACAAGAGAA AAACCTGAAA CTAATAGAGC AAAAACTAGG TATGACTCCC 
ACACGTAAGT GTAGTGATTC ATTGCGTCAT CCAATGGCTG ATTGTCCAAT TCCTCCTAAA 
AGAATGAGAA GAGACACTAC AAGACAAAAT AGGATTATAA CCAAAACAGA TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023503317.1
CDS	139..6135
Translation	MAAAGGGGPSVGTGEGGGGDGYRTVYLFDRREKESELGDRVLKVGEGSDYAGFRASVCQTLGISPEEKFVITTTSRKEVTCDNFDETVKDGVTLYLLQSVNQLLQTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNTGIRRIQIKLHFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIQNRKPSDSVHITNDDERFLHHLISEENEKDSFTAVVITGVQPEHIQYLKNYFPLWTRQLAHIYHYYIHGPKGNEKRTSKEVGPFNNIDIEISMFEKGKAPKIVNLREIQDDMQTLYINTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDDDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTLFKGIITRPDLPSKKQGPWATYSAIEWDGKIYKAGQLVKTIKTLPLFYGSIVKFFLYGDHDGEVYATGGEVQIAMEPQALYDETRTVPIAKLDRTVAEKTVKKYVEDEMARLPDRLSVTWPEGDELLPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLLDPPFRVGVPFNIPLEFQDEFGHTSQLINDIQPVLEASGLSLHYEEITKGPNCVIRGVTAKGPVNSCQGKNFNLKVILPGLKEDSQILKIRLLPGPPRRLKVKPDSEILVIENGTAFPFQVEVLDESDNVTAQPKLIVHCKFLGAPNLPVYVVDCSSSGTSILSGSAIQVQNIKKDQTLKAKIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDIMHNLIFQMYDEGEREIHITSALAEKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCELKGGKTVQMGQELQGEIVIIVTDQYGNQIQAFSPSSLSALGIAGIGLDSSRLKTTFQENTQSISVRGVKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKLLLIDWPELKESIPVINGRELQHPLIVQLCDQWDNPAPVPQVKISLIKANYLKLTPSNQQHKTDERGKANLGVFSVYAPRGEHTIQIKAVYNKTTIEGPIIKLMILPDPEKPIRLNVKYDKDASFLAGGVFTDFMITVISEDDSIIKNINPARITMKMWKLSNSGSRPPANAETFSCNKIKDNDKEDGCFYFRDKAIPNKVGTYCIQFGFMMDKANILNSEQVIVDVLPNQPVKLVPEIQPATPAVSNVRSVASRTLVKDLHLNITDDYNNYTGIDLVGTVIATIKGSNEEDNDTPLFIGKVRTLEFPFVKGSAEITSLVLAENSPGRDSTEYFIIFEPRLPALSRKLESYILPFMFYNDVKKQQQMAELTKEKDQLSKSIIMYRSLFEASKQLLDEMKCQVEEAKLKEAQLRNELKAHNIDIPTTQQMPHIEALLKRKTSEQEELRKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRPLPHYRNGKLYFKPVGDPVFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPIPKQCLILGEQIDLLQQYRTAVCKLGAVNDDLNGQLEYLHTPDMKKKKQELDEQEKNLKLIEQKLGMTPTRKCSDSLRHPMADCPIPPKRMRRDTTRQNRIITKTDV

Target ORF information:

RefSeq Version	XM_023647549.1
Organism	Equus caballus(horse)
Definition	Equus caballus structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023647549.1

ORF Insert Sequence:

ATGGCAGCGG CGGGCGGCGG CGGGCCCTCG GTGGGGACTG GAGAGGGCGG CGGCGGCGAC 
GGCTACAGGA CAGTGTACTT GTTTGACCGG CGCGAGAAGG AGTCCGAGCT CGGGGACCGG 
GTGCTGAAGG TCGGGGAGGG CTCGGACTAC GCGGGGTTCC GCGCCTCCGT GTGTCAGACA 
CTTGGCATTT CACCTGAAGA GAAATTTGTT ATTACAACAA CAAGTAGGAA AGAAGTTACC 
TGTGATAATT TTGATGAGAC TGTTAAAGAT GGAGTCACTC TATACCTGCT ACAGTCAGTC 
AATCAGTTAC TACAAACAGC TACAAAAGAA CGAATTGACT TCCTACCTCA CTATGACACA 
CTGGTTAAAA GTGGCATGTA TGAATATTAT GCAAGTGAAG GACAAAATCC TTTACCATTT 
GCTCTTGCAG AATTGATTGA CAATTCATTG TCTGCTACTT CTCGTAACAC TGGTATTAGA 
AGAATACAGA TCAAATTGCA TTTTGATGAA ACACAAGGAA AACCTGCCGT TGCAGTGATA 
GATAATGGAA GAGGAATGAC CTCTAAACAG CTTAACAACT GGGCCGTGTA TAGGTTGTCA 
AAATTTACAA GGCAAGGTGA CTTTGAAAGT GATCATTCGG GATATGTTCG ACCAGTACCA 
GTGCCACGCA GTTTAAACAG TGATATTTCC TATTTTGGTG TTGGGGGCAA GCAGGCTGTT 
TTCTTTGTTG GACAGTCAGC CAGAATGATA AGCAAACCTG CAGATTCCCA AGATGTTCAT 
GAGCTTGTGC TTTCTAAAGA AGATTTTGAG AAGAAGGAGA AAAACAAAGA GGCAATATAT 
AGTGGATATA TTCAAAACAG AAAGCCATCT GATTCTGTTC ACATTACAAA TGATGATGAA 
AGATTTCTAC ATCATCTTAT CTCAGAGGAG AACGAAAAAG ATAGCTTTAC TGCTGTGGTT 
ATCACAGGAG TACAACCAGA ACACATACAA TACTTGAAAA ATTATTTCCC TCTTTGGACA 
AGACAGTTAG CGCATATTTA TCATTACTAT ATTCATGGTC CAAAAGGAAA TGAAAAACGT 
ACATCAAAAG AAGTTGGACC TTTCAACAAC ATTGATATTG AAATTTCTAT GTTTGAAAAG 
GGGAAGGCAC CTAAGATTGT CAACCTAAGG GAAATACAAG ATGACATGCA GACGTTGTAT 
ATAAACACAG CAGCTGATAG CTTTGAGTTC AAGGCTCATG TTGAAGGAGA CGGTGTAGTG 
GAAGGGATTA TCCGATATCA CCCTTTCTTA TATGATAGAG AAACTTACCC TGATGATCCA 
TGCTTTCCAT CAAAATTAAA AGATGAAGAT GATGATGATG ATTGTTTCAT ACTTGAGAAA 
GCAGCAAGAG GGAAAAGGCC TATTTTTGAA TGTTTTTGGA ATGGACGACT AATACCATAT 
ACTTCTGTTG AAGACTTTGA CTGGTGTACT CCTCCTAAGA AGAGAGGGCT TGCACCAATT 
GAGTGCTACA ATAGAATATC TGGTGCATTA TTCACTAATG ACAAATTCCA GGTCAGCACA 
AATAAATTGA CTTTTATGGA TCTTGAGCTA AAACTGAAAG ATAAGAACAC CCTTTTTACA 
AGGATTTTAA ATGGACAGGA GCAGCGAATG AAAATTGACA GAGAATTTGC TCTATGGCTG 
AAGGACTGTC ATGAAAAGTA TGACAAACAA ATAAAATTTA CACTTTTTAA AGGAATAATT 
ACACGTCCTG ATCTTCCTTC TAAAAAGCAG GGCCCCTGGG CGACATACTC AGCAATAGAA 
TGGGATGGAA AAATATACAA AGCAGGACAG CTGGTCAAGA CAATCAAGAC ACTGCCTCTC 
TTTTATGGAA GCATAGTAAA ATTTTTTCTT TATGGTGATC ATGATGGAGA AGTATATGCT 
ACAGGAGGAG AGGTTCAAAT TGCAATGGAA CCTCAGGCAC TGTATGATGA AACAAGAACT 
GTGCCAATTG CAAAACTGGA TAGGACGGTT GCTGAGAAGA CTGTAAAAAA ATACGTGGAA 
GATGAAATGG CAAGGCTCCC TGATAGATTG TCAGTAACTT GGCCTGAAGG AGATGAATTA 
TTGCCAAATG AGGTTAGGCC TGCTGGAACA CCCATTGGTG CATTAAGAAT TGAAATACTG 
AATAAAAAAG GGGAAGCAAT GCAGAAGCTT CCAGGAACAA GTCATGGAGG GTCAAAGAAA 
CTCTTGGTTG AGCTCAAAGT TATATTACAC TCTTCAAGTG GAAATAAAGA AATAATTTCA 
CATATTAGTC AGCATGGAGG AAAATGGCCT TACTGGTTTA AAAAAATGGA AAATATTCAA 
AAGTTGGGGA ATTATACACT GAAATTACAA GTTGTATTGA ATGAAAGTAA TGCAGACACT 
TATGCAGGAA GACCATTACC ATCTAAAGCA ATTAAATTTT CTGTTAAAGA GGGTAAGCCA 
GAGAAATTTT CATTTGGTCT TCTGGATCCT CCTTTTCGTG TTGGAGTCCC ATTCAATATT 
CCTCTGGAGT TTCAAGATGA ATTTGGTCAT ACTAGTCAAC TAATAAATGA TATTCAGCCA 
GTTCTTGAAG CAAGTGGTTT ATCTTTACAT TATGAAGAAA TAACCAAAGG ACCGAATTGT 
GTAATCCGAG GTGTTACAGC TAAGGGCCCT GTAAACTCTT GTCAAGGCAA GAATTTTAAT 
CTGAAGGTTA TTCTGCCGGG TTTAAAAGAA GACTCACAGA TTTTGAAAAT TAGATTACTA 
CCAGGTCCCC CTCGTCGATT GAAAGTGAAA CCTGATTCTG AAATTTTAGT TATAGAAAAT 
GGAACAGCTT TCCCATTTCA GGTAGAAGTT TTGGATGAAT CAGACAATGT AACAGCACAA 
CCAAAACTGA TTGTTCATTG TAAGTTTTTA GGTGCTCCAA ACCTTCCAGT TTATGTTGTG 
GATTGCAGTA GTTCTGGAAC CAGTATTTTA TCAGGATCTG CAATTCAAGT TCAGAATATT 
AAAAAAGACC AGACGCTTAA GGCTAAAATT GAAATACCTA GTTGTAAAGA TGTAGCCCCT 
GTGGAGAAGA CTATTAAGTT GCTTCCCAGT AGCCATGTTG CAAGATTACA GATTTTCAGT 
GTGGAAGGAC AAAAGGCAAT CCAGATAAAA CATCAGGATG AGGTCAATTG GATAGCAGGC 
GATATTATGC ACAACCTTAT TTTTCAAATG TATGATGAAG GAGAAAGAGA AATCCATATA 
ACATCAGCTT TAGCAGAGAA AATTAAAGTT AATTGGACTC CTGAGATTAA CAAAGAACAC 
TTGCTGCAGG GTCTGCTTCC TGATGTACAA GTACCAACGT CTGTAAAAGA TATGCGTTAT 
TGCCAGGTTT CATTCCAAGA TGATCATGTG TCTTTGGAAA GTGCATTTAC AGTAAGACCA 
CTTCCTGATG AACCTAAACA TCTAAAATGT GAGTTGAAAG GAGGAAAAAC AGTACAGATG 
GGCCAAGAGC TTCAAGGAGA AATAGTTATA ATAGTTACAG ATCAATATGG AAATCAGATT 
CAAGCATTTT CACCAAGTTC TTTATCTGCC TTGGGAATTG CTGGGATTGG ACTTGATAGC 
TCACGTTTGA AAACTACTTT TCAGGAAAAT ACACAAAGTA TAAGTGTAAG AGGCGTCAAA 
TTTATTCCAG GTCCTCCTGG AAATAAGGAT CTTTGTTTTA CTTGGCGTGA GTTTTCTGAC 
TTTATTCGAG TGCAGCTAAT TTCTGGACCA CCAGCTAAAC TTCTCCTCAT AGACTGGCCA 
GAATTAAAGG AGTCCATTCC GGTGATTAAT GGAAGAGAAT TACAGCATCC TCTCATTGTT 
CAACTTTGTG ATCAGTGGGA TAATCCAGCA CCCGTACCAC AAGTTAAAAT AAGTCTCATA 
AAAGCTAACT ACTTAAAACT CACCCCTTCA AACCAACAGC ATAAAACAGA TGAAAGGGGC 
AAGGCTAATT TGGGAGTGTT CAGTGTTTAT GCCCCTAGAG GAGAGCATAC TATTCAGATT 
AAAGCCGTCT ATAACAAGAC TACCATAGAA GGACCTATAA TTAAGTTAAT GATTCTTCCA 
GACCCTGAAA AACCCATTCG TCTCAATGTT AAATATGACA AAGATGCTTC CTTTTTAGCA 
GGAGGTGTTT TCACTGATTT TATGATTACT GTTATTTCTG AAGATGACAG TATCATTAAA 
AATATTAATC CAGCACGTAT TACTATGAAA ATGTGGAAGC TGTCTAACAG TGGGAGCCGA 
CCACCAGCAA ATGCAGAAAC ATTCAGTTGT AATAAAATAA AAGATAATGA CAAGGAAGAT 
GGCTGCTTCT ACTTCAGGGA TAAAGCAATT CCTAACAAAG TGGGGACATA TTGTATCCAG 
TTTGGTTTTA TGATGGATAA AGCAAATATT CTTAACAGTG AACAGGTTAT AGTTGATGTT 
CTGCCTAATC AACCTGTGAA GTTAGTTCCG GAAATTCAGC CAGCTACACC AGCTGTTTCA 
AATGTTCGCT CAGTTGCCAG TAGGACCTTA GTCAAAGATT TACATCTTAA TATCACGGAT 
GACTACAACA ATTATACCGG AATTGATTTG GTTGGCACTG TAATAGCTAC CATCAAAGGT 
TCTAATGAGG AAGATAATGA TACACCTCTC TTTATTGGAA AAGTTAGAAC ACTTGAATTT 
CCCTTTGTGA AAGGTTCAGC TGAAATCACG AGCCTAGTGC TGGCAGAAAA TAGTCCTGGA 
AGGGATAGTA CCGAATATTT TATTATATTT GAGCCTCGGC TACCAGCTTT ATCAAGAAAA 
TTAGAATCAT ATATCCTACC GTTTATGTTT TACAATGATG TTAAGAAGCA GCAACAAATG 
GCAGAACTTA CTAAAGAAAA GGACCAATTA TCTAAATCTA TTATAATGTA TAGAAGTTTA 
TTTGAAGCCA GCAAACAGCT TCTTGATGAA ATGAAGTGCC AAGTTGAAGA AGCAAAATTA 
AAAGAGGCCC AGTTGAGAAA TGAACTAAAA GCACATAATA TTGACATTCC TACAACACAA 
CAGATGCCAC ACATTGAAGC GCTTTTGAAA AGAAAGACAT CAGAACAGGA AGAACTAAGG 
AAAAAACCTA GAAGATCATG TACTCTTCCA AACTACACTA AAGGCAGTGG AGATGTTTTG 
GGAAAGATTG CACACCTGGC ACAAATTGAA GATGATAGAG CTGCCATGGT TATTTCTTGG 
CATCTAGCAA GTGACATGGA CTGTGTGGTC ACCCTGACCA CTGATGCTGC ACGTCGTATC 
TATGATGAAA CACAAGGTCG TCAGCAGGTG TTGCCCCTTG ATTCTATTTA TAAGAAGACT 
CTTCCAGATT GGAAAAGACC TCTACCTCAT TACCGAAATG GAAAATTATA TTTTAAACCC 
GTTGGAGATC CAGTCTTTGC CCGAGATTTG TTAACATTTC CAGATAACGT AGAACATTGT 
GAAACAGTAT TTGGTATGCT GTTAGGAGAC ACCATTATTT TGGATAATTT GGATGCTGCC 
AATCATTATA GAAAAGAGGT TGTTAAAATT ACACACTGTC CTACACTGCT GACCAGAGAT 
GGAGATCGCA TTCGAAGTAA TGGAAAGTTT GGCGGCCTTC AAAATAAAGC TCCTCCAATG 
GATAAACTTC GGGGAATGGT ATTTGGAGCT CCAATCCCAA AACAGTGCCT GATCTTAGGG 
GAACAAATAG ATCTTCTTCA GCAGTATCGT ACTGCTGTGT GCAAGTTGGG TGCTGTGAAT 
GATGACCTTA ATGGTCAGTT AGAGTACCTT CACACGCCTG ATATGAAGAA GAAAAAACAA 
GAACTCGATG AACAAGAGAA AAACCTGAAA CTAATAGAGC AAAAACTAGG TATGACTCCC 
ACACGTAAGT GTAGTGATTC ATTGCGTCAT CCAATGGCTG ATTGTCCAAT TCCTCCTAAA 
AGAATGAGAA GAGACACTAC AAGACAAAAT AGGATTATAA CCAAAACAGA TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SMCHD1 cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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