SPAG17 cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAG17
Entrez Gene ID	100059849
Full Name	sperm associated antigen 17
General protein information	Preferred Names sperm associated antigen 17 Names sperm-associated antigen 17
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001500885.4	XP_001500935.1	sperm-associated antigen 17 isoform X1
XM_001500885.3	XP_001500935.1	sperm-associated antigen 17
XM_023641331.1	XP_023497099.1	sperm-associated antigen 17 isoform X4
XM_023641332.1	XP_023497100.1	sperm-associated antigen 17 isoform X5
XM_023641329.1	XP_023497097.1	sperm-associated antigen 17 isoform X2
XM_023641330.1	XP_023497098.1	sperm-associated antigen 17 isoform X3

Pathways from BioSystems
Gene Ontology

The following SPAG17 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAG17 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHb21198	XM_001500885.4 Latest version!	Equus caballus sperm associated antigen 17 (SPAG17), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb65449	XM_023641329.1 Latest version!	Equus caballus sperm associated antigen 17 (SPAG17), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb65450	XM_023641330.1 Latest version!	Equus caballus sperm associated antigen 17 (SPAG17), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb65451	XM_023641331.1 Latest version!	Equus caballus sperm associated antigen 17 (SPAG17), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHb65452	XM_023641332.1 Latest version!	Equus caballus sperm associated antigen 17 (SPAG17), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OHb21198	XM_001500885.3	Equus caballus sperm associated antigen 17 (SPAG17), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAG17 ( XM_001500885.4 ) cDNA ORF clone, Equus caballus(horse) -> XP_001500935.1 Equus caballus sperm associated antigen 17 (SPAG17), transcript variant X1, mRNA.

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CloneID	OHb21198
Clone ID Related Accession (Same CDS sequence)	XM_001500885.4 , XM_001500885.3
Accession Version	XM_001500885.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	sperm-associated antigen 17 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_001500885.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCA AGAAAGAGAA AAGCGGGTCG GCGAGCGCGA ATTCTAAGAT ATGGGAACCC 
TCGCTCATCG CTGCACACTT CAATCAGAAC GATTGGAAGG GCGTCATCGC TTTTGTAGTT 
GGAAACCGGA TTGAGGATGA GCTTCTCATT GGAGCCCTCA CCCTCGCTGT CCAGGCCCCT 
CAGCGTAGAC TCTTCAGTAT GGTATCATGG CAAGACATCC TCCAGCAGAT CAATGGAACA 
AATGCGCTTC CTGGAACTGC ATCTAAAAAG GGAAAGAAAC CTCTAGGCGG TAGCGCTCCC 
TTATATTCTG AGGTGTTAAT AGCAACAAAG GCAATTATGG ACAGTGGAGA GAAATTAACC 
TTACCGCTGA TAGGGAAACT CTTGAAATAT CAGCTTCTCC AGATTAAATA TAAGGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGGTGACA GAAGACAAAT TTAAGACTGA AAAGGATAAA 
GGGAAAGCGA AATCTCCCAA GGAAAAGAAG CCCTCAGGTA CCAAGGTTGT CAAAGGAAAG 
GGAAAGGATC AGCCTGAGGC AAACACAACA GTGAAAAAGG CCACTCAGTT AAAACAGAGG 
GGAGAAGAAG ACGACGCCAA ACGTTACATT GATGATGAGC CAGATGATGG TGCCCAACAT 
TACATTATAG TTGTGGGCTT CAACGATCCT CAGCTATTAG CGATTATGAC TGAGCTTGGC 
ATTCCCATAA GCAGCGTGAT TAAAATATCT TCAGAGAATT ATGAACCTCT GCAGGCGCAC 
CTGGCAGCAG TTAGCCAGCA GCAGGAAGTT TCTCTTCAGC CAGAAGATAT AGAAGCTGAA 
AAATTGAAGA ATGAGAATAC CATAAAAGAA CTTGAAATTT TCTGGAAGTA CTTGGAACCA 
ATCCTGAATA ATGAGAAACC TGAAACAAAT CTCTTTGATG TTGCTCGTCT TGAATACATG 
GTCAAGGAAG ATGGCTTTCC TTCTGACTGG TCAGACAGTG GGATGATGCT GGAATTGGGC 
ACCGACATTT TTGAAAATAT TGCCTGTTTG ATGTACGACA GCCTGGATTG GAAAAGGCAG 
CACCGGCACT ATTTAGAAAG CATGCAGCTT ATTAATGTCC CACAAGTGGT TAACGAGAAG 
CCTATCTTAG AAGCTGTAGC CATTGCTGAG GCTCCACAAG CTGCTGCTCC AACCCCTGGG 
AAGAAGAAAG CACAAAATGA AGAACGGCAA GCCCCAACAT CAGTGCCTTT TGTCATCACA 
ACTGAAGCAG ACATGAGATA TTACAATGAT TTGCTGAATC CGATTCCAGA AGAATTCATT 
TCTGTGCCCC TGATACTGCA CTGTATGCTG GAACAGGTTG TAGCAACTGA AGAAGACCTT 
GTCCCACCCA GTCTGGTGGA GCCGCCTCCC AGAGCAGATG GGTTGGACCA CAGAATTGCA 
GCTCACATTG TATCCATCCT ACCCTCTCTC TGTCTCACGG AGAAGGAGAA AAAGAATCTC 
CAGGAAATAT TTTTGTCTGA AGAAGAAAAT GAAAGCAAAG CAGTGCCCAA AGGTCCTCTC 
CTACTGAACT ATCACGACAC TCTGGCCCAC AAGAAGTATG CACTGAAGGA CCCAAAGAAT 
TTCGACCCAG TTCAAATTGA GCAGGAGATG CAGTCCAAGT TGCCACTGTG GGAATTCCTT 
GAATTCCCTC TGCCCCCGCC ATGGAACAGC ACTAAACGTC TAGCTACAAT TCATGAGCTC 
ATGCACTTTT GTACAAATGA AGTCTTGAGC TGGAATGAAG TAGAACGAGC CTTCAAAGTG 
TTTACTTTTG AGAGCCTGAA ACTCTCTACA GTTGATGAAA AAGGGAAACT AAAGCCTGCT 
CGGATGATGT ATGGGACTGA TTGTGAGATG TTCAACATAC CATGGGACAA CCCTGCCAGA 
TTTGCTAAAC AGATAAGGCA ACAATACATC ACGAAAATGA ACACCCAAGA GGTCAAACAG 
AAAACAGATA CTGAAACCAA AGACAGAATT TTGTTTGTGG ATCAGAATAT GCCAGTGTCT 
GTGCAAGATG ATGAGAGCAG TAAAGAACCT TCAGACCCTA GTCAGGCTGA TGCTAGCAAT 
ATGCAGCATT CTGACTCGAA TAATAAGAAA TCCTTAGACC CTGATGATAG AGAGCTGTCA 
GAGCAGGACA ACAGCTTGAA GACTCAGCTC CAATCTGAGC CTTTGGAGCA GACCACGGGC 
AATGAGATCA AAGATGAAGC AGTCACAAAT GTTGATCCTC CTGAAAAGAA ATCCAAGAAG 
ATGACCGTGG AAGCAGAGTT GGAGGACATA AAGAAAACAG AGCAACGCAG CCTAATGGAT 
TGGAGTTTCA CTGAACATTT TAAACCGAAA GTTCTACTGC AGGTTCTTCA AGAAGCCAAT 
CAGCATTACA GATGTATTGA TTCTTATTAC CACACCCAAG ACAACTCTTT ACTATTAGTC 
TTTCATAATC CAATGAATTT ACAACGTCTG CATTGTGAAT ATTGGAATGT TGCTCTTCAC 
TCCAATGTTG GATTCAGGAA TTATTTGGAA CTTGTTGCAC AGTCCATTCA AGACTGGATC 
ATAAAAGAAG AAGCTATATA TCAGGAATCT AAAATGGCTG AGGAACTCAG CAGGAGCAAG 
CTTGAGCAGG AAGTGAAATC TCCTGCTAGT GGCAAAATTT CTTCCCCTAG CAAAATTTAT 
TCTGTTAAAA AATCTAAAAG TAACAAAGGA ATGAGCAAAA CAGAGTTAAT AGATCAAGAA 
AAAGAAAAAG AGAAAGAGAA GGAAAAGATT CCTTTCATTT TAGAAGGCTC TCTCAAGGCA 
TGGAAAGAAG AGCAAGATCG ATTAGCAGAA GAGGAACGCT TAAAGGAAGA AAAAAAAGCA 
GAGAAGAAGA GTAAGGACAT TGGTAAAAAG AAAGGCAAGG AAAAGGTAGA GAGAGAAGAT 
AGCAGGGTTT TGAAGAAAAA ATCTTTTTTC AAGGATAAAG CTAAAGAAGA GCAAATCAAG 
ATCTCAGACG TAACAGAGGA GCCCCTTCAA CAGCCAGAAC CTGAGATAGT TTACCCGTTT 
CGCGGATACA ATATGGGAGA TATACCCATC CAAATCTCGG GAACAAATTG TTATCTGTAT 
CCTTCAGATG GAGGGCAGAT TGAAGTAGAA AAGACAACAT TTGAAAAAGG TCCAACTTTT 
ATCAAAGTGA AAGTGAAAAA GGACAAGCAC AATTTTATAA TTCATTTAAA AGATCCTAAG 
AAAATTGTGA GAAACGAAAA AAAAGAAGAC GATTATTCTT CAGAAGATGA GAAAGATGAA 
GGAGAGGAAG AACGAAATCT TGAAATGAAA GAATCAGATG CAGAGAATAA AGCTATCAGC 
AAGTTTGGAT CTTTTTCTGC CACCTTGGAA AATGGAATCT GCCTCTCAAT AAGTTACTAC 
GGATCAAGTG GAATGGCGCC AGAGGATAAG GATCCTAATT TGGAAGCAAT GTTGAATATC 
CCGTCAGCAC TCACTCCAAC AATAATTCCT GTGGTAGTGA CTGTTCCTCA AGGCAAAGGA 
AAAGGGAAAA CCAAAGGCAA AGAAAAGCCC AAAGACTCTA TTAAAGAAGA GGAACATCCA 
AAAGAAGAAG AGAAAAAGGA AGAAGTAGAA CCAGAACCTG TTTTACAAGA GACTGTTTAT 
GTTCCCACCT TCCAAAACCT AAATGTGTCT TGCCCCAGTG GGCTTCTAGT GACCTTCATT 
GGCCAAGAGT CTGCAGATCA CCATGTTATG GATGAGGAAC CCACTTGGGA CATCATGGTC 
CGACAGAGCT ACCCCCAGAA GGTGAAGCAC TATGAGTTCT ACAAAACGGT GATGCCACCA 
GTGGAGCAGG AGGCATCGAG AGTTATCACT AGTCAAGGCA CTGTTGTCAA ATACATGTTG 
GATGGATCCA CACAGATTCT CTTTGCAAAT GGCGCTGTGA GCAGTAGTCC CGATTCAGGT 
CCTGTTTGTC CTCCTCCTGA AATGCCAGCG AGCCCTCATA ACGGAGACTT GATGGACAGT 
GTTTCTCAGC AAAAATCAGA AACAGCACCG TCTGAGACTG TCATCACAAA GAAAGGAAAA 
GGTCACAAAA ATCAGTCATC AGTGCCACAT ACGAATGAAA TCCATGAACC TCCCCAAGAG 
GTAGTTCAAA TAGTAACACC TGTGGAGGTT CATGTAGGCA CCTGGTTTAC AACTACACCT 
GACGGAAATC GGATTGGCAC CAAAGGATTA GAAAAAATAG CAGACTTGGC CCCATTCTTA 
TCCTTTCAGG CCACAGATCC TGTCAATGGA ACGGTTATGA CAACTCGAGA GGACAAGGTG 
ATCATTGTTG AAAAGAAGGA TGGTACTCGG ATCGTGGATC ATGCCGATGG TACCAGAATC 
ACAACCTTTT ATCAAGTTTA TGAAGATCAT ATTATGCCTC CAGATGATCA AGAAATACCC 
GAACATCCTC AAACTCTCAC CAGGCAGGTG AAGTGCATGC GCATAGAAAG CTCACACTAT 
GCCACTGTCA TCACCAACTG CGAGGACAGT AGCTGCTGTG CCACTTTTGG AGATGGGACG 
TCTATTATTG CAAAGCCACA GGGAACATAC CAGGTGTTAC CTTCAAACAA AGGCTGTCTT 
CATATTGATA GAGACTGTTC AGCTGTATAT TACCATGAAT CCAGCAGTGA TATATACCAT 
CCTTTCCAAA AACGTGAGCA GCTGAGAGCT AGCAGGTACA TCATGAAGCA CACGTCAGAG 
GTTCTCTGTG AAGTCCTGGA TCCTGAGGGA AACACCTTTC AGGTCATGGC TGATGGTAGC 
ACATCAACTC TGCTACCTGA AAACAATTTG GAAGATGATT TAAACTCAAA ACCTGAAGGC 
TATGATAGTT TATCATCTAC TCACCTTCAT AAAAATCACC TACAAATATA TGGTGAACAT 
GTCCCCAGGT TTTTTGTTAT CTATGCCGAT GGATCAGGAG TAGAACTTCT TCGAGATGAT 
GACATAGAAG AATACTTATC CTTGGCATAT GGAGAATCAA CCACTGTAGT TCTTCAAGAG 
CCAGTACAAG AACAGCCAGG CGCCCTGAGC ATCACTGTTC TTCGCCCTTT TCATGAAGCA 
TCGCCATGGC TAATGAAGAA AGAATTAGAC ACAATTGTTC CTCCTAATCT CCAGTCAAGG 
TCATGGGAGA AATTTCCCTC AGTTGAGAAA AAAACTCCAG GACCTCCCTT TGGCACTCAT 
ATTTGGAAGG GCCTTAACAT TGAGTGCAAA CAGCTAGTGA GTGCCCCAGC TCCTATACTC 
AAAAGCCCCA ATGTACTACA GATACGCCAG TTCATCCAGC ATGAGGTCAT AAACGATGAG 
GTGAAACTGA AGCTGCAGAT TTCCCTTAAG GACTATATAA ACCATATTTT AAAGAAAGAA 
GATGAGCTAC AGGAAATGAC AGTTAAAGAT TCCAGAACTG AGGAGGAGAG AGGCAATGCT 
GCAGATCTCC TCAAGCTTGT TATGTCTTTT CCTAAAATGG AGGAAACTAC AAAAGGTCAT 
GCTACTGAAG TTGCAGCCCA CTTAGTTGAT TTATTCAAGC AATCTTTGGC TGTTGCTCCA 
GAATGTCCAC CAGAAACATT TGATACAGAT TTCTTGGAAA AGAAAGGGAG GCACATAGCA 
AGTGAAAAGC GCTGGAAAGA AAAGATAGAT CAAAAGAGGA AGGAAATCGA AAAAACACAC 
AATTATCTAG AGGAAATCAG GAACAGAACG GTATCACCCT ACTTCAAATC AGAATTGAGC 
AAGACCTTTC AGTCTCAGTT TAATTATCTA GACGCCCTTT CCAAAAACTT GCCTCCTTTT 
ACAAAGAAAG TTGAAGATAC AAACAAAACA GCTGTTCAAA ATACATCCAA TCTTCAGCCA 
GATTCCCAGC CAAATAAAGT TTCACAACAA GAATCCTCAG ACACACTTAG TCTTCCAAAA 
TCAGAGGCTT TTGTGGATAT GAAGGCGTTC GCTAGCCAGA ACCAAACTGA AAATTTAACA 
AAATCTACTG AAGAACCAGA AACTTATGCC CCTGTGAAAA TTCCAACCCA GTCATTGCTG 
CAGGATGTTG CAGGACAAGC AAGGAAAGAG AAAGTGAGGT TACCTCGCTA TTTGCTGAGT 
TCCAAACCCA AGTCTCAACC TCTCGAGAAG GTGCAAGATT CTGTTGGAGG AAGAGTGAGA 
ACATCCTCGG TTGCTTCTGC TGCCATTAAT AACGCAAATT CATCCCCTTT TGGATTTCAT 
CTTCTCCCAC CGTCAGTGAA TTTTGGAGTG CTTGAAGAAG GGCACACCTA TGAAACCACT 
GTGAAGCTCA AGAATGTTGG AGTGGACTTC TGCAGGTTTA GGGTGAAGCA GCCGCCACCC 
AGCACAGGAT TGAAAGTGAC TTACAAACCT GGGCCTGTGG CAGCTGGTTT GCAGGCAGAA 
CTGAAAGTGG AGTTATTTAC CATGGTTGTT GGAGAGGATG GTGCCAAGGG ATCAGCACAC 
ATCTCTCACA ATATTGAGAT TATGACTGAG CATGATGTCC TGTTCTTACC TGTGGAAGCA 
ACAGTTTTAA CAAGCAGCAA TTATGATAAG CGATTAAAAG ATTTTCCCCA GGAAAAAGAA 
AACCCCACGA TCCAGAGGAC TTCTACAATT TCTTCTGCTA AACTTGGAGT CTTCATGTCT 
CGTAAAGTTT CTCCTTATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001500935.1
CDS	67..6747
Translation	MAPKKEKSGSASANSKIWEPSLIAAHFNQNDWKGVIAFVVGNRIEDELLIGALTLAVQAPQRRLFSMVSWQDILQQINGTNALPGTASKKGKKPLGGSAPLYSEVLIATKAIMDSGEKLTLPLIGKLLKYQLLQIKYKDQQRRESEKKVTEDKFKTEKDKGKAKSPKEKKPSGTKVVKGKGKDQPEANTTVKKATQLKQRGEEDDAKRYIDDEPDDGAQHYIIVVGFNDPQLLAIMTELGIPISSVIKISSENYEPLQAHLAAVSQQQEVSLQPEDIEAEKLKNENTIKELEIFWKYLEPILNNEKPETNLFDVARLEYMVKEDGFPSDWSDSGMMLELGTDIFENIACLMYDSLDWKRQHRHYLESMQLINVPQVVNEKPILEAVAIAEAPQAAAPTPGKKKAQNEERQAPTSVPFVITTEADMRYYNDLLNPIPEEFISVPLILHCMLEQVVATEEDLVPPSLVEPPPRADGLDHRIAAHIVSILPSLCLTEKEKKNLQEIFLSEEENESKAVPKGPLLLNYHDTLAHKKYALKDPKNFDPVQIEQEMQSKLPLWEFLEFPLPPPWNSTKRLATIHELMHFCTNEVLSWNEVERAFKVFTFESLKLSTVDEKGKLKPARMMYGTDCEMFNIPWDNPARFAKQIRQQYITKMNTQEVKQKTDTETKDRILFVDQNMPVSVQDDESSKEPSDPSQADASNMQHSDSNNKKSLDPDDRELSEQDNSLKTQLQSEPLEQTTGNEIKDEAVTNVDPPEKKSKKMTVEAELEDIKKTEQRSLMDWSFTEHFKPKVLLQVLQEANQHYRCIDSYYHTQDNSLLLVFHNPMNLQRLHCEYWNVALHSNVGFRNYLELVAQSIQDWIIKEEAIYQESKMAEELSRSKLEQEVKSPASGKISSPSKIYSVKKSKSNKGMSKTELIDQEKEKEKEKEKIPFILEGSLKAWKEEQDRLAEEERLKEEKKAEKKSKDIGKKKGKEKVEREDSRVLKKKSFFKDKAKEEQIKISDVTEEPLQQPEPEIVYPFRGYNMGDIPIQISGTNCYLYPSDGGQIEVEKTTFEKGPTFIKVKVKKDKHNFIIHLKDPKKIVRNEKKEDDYSSEDEKDEGEEERNLEMKESDAENKAISKFGSFSATLENGICLSISYYGSSGMAPEDKDPNLEAMLNIPSALTPTIIPVVVTVPQGKGKGKTKGKEKPKDSIKEEEHPKEEEKKEEVEPEPVLQETVYVPTFQNLNVSCPSGLLVTFIGQESADHHVMDEEPTWDIMVRQSYPQKVKHYEFYKTVMPPVEQEASRVITSQGTVVKYMLDGSTQILFANGAVSSSPDSGPVCPPPEMPASPHNGDLMDSVSQQKSETAPSETVITKKGKGHKNQSSVPHTNEIHEPPQEVVQIVTPVEVHVGTWFTTTPDGNRIGTKGLEKIADLAPFLSFQATDPVNGTVMTTREDKVIIVEKKDGTRIVDHADGTRITTFYQVYEDHIMPPDDQEIPEHPQTLTRQVKCMRIESSHYATVITNCEDSSCCATFGDGTSIIAKPQGTYQVLPSNKGCLHIDRDCSAVYYHESSSDIYHPFQKREQLRASRYIMKHTSEVLCEVLDPEGNTFQVMADGSTSTLLPENNLEDDLNSKPEGYDSLSSTHLHKNHLQIYGEHVPRFFVIYADGSGVELLRDDDIEEYLSLAYGESTTVVLQEPVQEQPGALSITVLRPFHEASPWLMKKELDTIVPPNLQSRSWEKFPSVEKKTPGPPFGTHIWKGLNIECKQLVSAPAPILKSPNVLQIRQFIQHEVINDEVKLKLQISLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKGHATEVAAHLVDLFKQSLAVAPECPPETFDTDFLEKKGRHIASEKRWKEKIDQKRKEIEKTHNYLEEIRNRTVSPYFKSELSKTFQSQFNYLDALSKNLPPFTKKVEDTNKTAVQNTSNLQPDSQPNKVSQQESSDTLSLPKSEAFVDMKAFASQNQTENLTKSTEEPETYAPVKIPTQSLLQDVAGQARKEKVRLPRYLLSSKPKSQPLEKVQDSVGGRVRTSSVASAAINNANSSPFGFHLLPPSVNFGVLEEGHTYETTVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQAELKVELFTMVVGEDGAKGSAHISHNIEIMTEHDVLFLPVEATVLTSSNYDKRLKDFPQEKENPTIQRTSTISSAKLGVFMSRKVSPY

Target ORF information:

RefSeq Version	XM_001500885.4
Organism	Equus caballus(horse)
Definition	Equus caballus sperm associated antigen 17 (SPAG17), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001500885.4

ORF Insert Sequence:

ATGGCACCCA AGAAAGAGAA AAGCGGGTCG GCGAGCGCGA ATTCTAAGAT ATGGGAACCC 
TCGCTCATCG CTGCACACTT CAATCAGAAC GATTGGAAGG GCGTCATCGC TTTTGTAGTT 
GGAAACCGGA TTGAGGATGA GCTTCTCATT GGAGCCCTCA CCCTCGCTGT CCAGGCCCCT 
CAGCGTAGAC TCTTCAGTAT GGTATCATGG CAAGACATCC TCCAGCAGAT CAATGGAACA 
AATGCGCTTC CTGGAACTGC ATCTAAAAAG GGAAAGAAAC CTCTAGGCGG TAGCGCTCCC 
TTATATTCTG AGGTGTTAAT AGCAACAAAG GCAATTATGG ACAGTGGAGA GAAATTAACC 
TTACCGCTGA TAGGGAAACT CTTGAAATAT CAGCTTCTCC AGATTAAATA TAAGGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGGTGACA GAAGACAAAT TTAAGACTGA AAAGGATAAA 
GGGAAAGCGA AATCTCCCAA GGAAAAGAAG CCCTCAGGTA CCAAGGTTGT CAAAGGAAAG 
GGAAAGGATC AGCCTGAGGC AAACACAACA GTGAAAAAGG CCACTCAGTT AAAACAGAGG 
GGAGAAGAAG ACGACGCCAA ACGTTACATT GATGATGAGC CAGATGATGG TGCCCAACAT 
TACATTATAG TTGTGGGCTT CAACGATCCT CAGCTATTAG CGATTATGAC TGAGCTTGGC 
ATTCCCATAA GCAGCGTGAT TAAAATATCT TCAGAGAATT ATGAACCTCT GCAGGCGCAC 
CTGGCAGCAG TTAGCCAGCA GCAGGAAGTT TCTCTTCAGC CAGAAGATAT AGAAGCTGAA 
AAATTGAAGA ATGAGAATAC CATAAAAGAA CTTGAAATTT TCTGGAAGTA CTTGGAACCA 
ATCCTGAATA ATGAGAAACC TGAAACAAAT CTCTTTGATG TTGCTCGTCT TGAATACATG 
GTCAAGGAAG ATGGCTTTCC TTCTGACTGG TCAGACAGTG GGATGATGCT GGAATTGGGC 
ACCGACATTT TTGAAAATAT TGCCTGTTTG ATGTACGACA GCCTGGATTG GAAAAGGCAG 
CACCGGCACT ATTTAGAAAG CATGCAGCTT ATTAATGTCC CACAAGTGGT TAACGAGAAG 
CCTATCTTAG AAGCTGTAGC CATTGCTGAG GCTCCACAAG CTGCTGCTCC AACCCCTGGG 
AAGAAGAAAG CACAAAATGA AGAACGGCAA GCCCCAACAT CAGTGCCTTT TGTCATCACA 
ACTGAAGCAG ACATGAGATA TTACAATGAT TTGCTGAATC CGATTCCAGA AGAATTCATT 
TCTGTGCCCC TGATACTGCA CTGTATGCTG GAACAGGTTG TAGCAACTGA AGAAGACCTT 
GTCCCACCCA GTCTGGTGGA GCCGCCTCCC AGAGCAGATG GGTTGGACCA CAGAATTGCA 
GCTCACATTG TATCCATCCT ACCCTCTCTC TGTCTCACGG AGAAGGAGAA AAAGAATCTC 
CAGGAAATAT TTTTGTCTGA AGAAGAAAAT GAAAGCAAAG CAGTGCCCAA AGGTCCTCTC 
CTACTGAACT ATCACGACAC TCTGGCCCAC AAGAAGTATG CACTGAAGGA CCCAAAGAAT 
TTCGACCCAG TTCAAATTGA GCAGGAGATG CAGTCCAAGT TGCCACTGTG GGAATTCCTT 
GAATTCCCTC TGCCCCCGCC ATGGAACAGC ACTAAACGTC TAGCTACAAT TCATGAGCTC 
ATGCACTTTT GTACAAATGA AGTCTTGAGC TGGAATGAAG TAGAACGAGC CTTCAAAGTG 
TTTACTTTTG AGAGCCTGAA ACTCTCTACA GTTGATGAAA AAGGGAAACT AAAGCCTGCT 
CGGATGATGT ATGGGACTGA TTGTGAGATG TTCAACATAC CATGGGACAA CCCTGCCAGA 
TTTGCTAAAC AGATAAGGCA ACAATACATC ACGAAAATGA ACACCCAAGA GGTCAAACAG 
AAAACAGATA CTGAAACCAA AGACAGAATT TTGTTTGTGG ATCAGAATAT GCCAGTGTCT 
GTGCAAGATG ATGAGAGCAG TAAAGAACCT TCAGACCCTA GTCAGGCTGA TGCTAGCAAT 
ATGCAGCATT CTGACTCGAA TAATAAGAAA TCCTTAGACC CTGATGATAG AGAGCTGTCA 
GAGCAGGACA ACAGCTTGAA GACTCAGCTC CAATCTGAGC CTTTGGAGCA GACCACGGGC 
AATGAGATCA AAGATGAAGC AGTCACAAAT GTTGATCCTC CTGAAAAGAA ATCCAAGAAG 
ATGACCGTGG AAGCAGAGTT GGAGGACATA AAGAAAACAG AGCAACGCAG CCTAATGGAT 
TGGAGTTTCA CTGAACATTT TAAACCGAAA GTTCTACTGC AGGTTCTTCA AGAAGCCAAT 
CAGCATTACA GATGTATTGA TTCTTATTAC CACACCCAAG ACAACTCTTT ACTATTAGTC 
TTTCATAATC CAATGAATTT ACAACGTCTG CATTGTGAAT ATTGGAATGT TGCTCTTCAC 
TCCAATGTTG GATTCAGGAA TTATTTGGAA CTTGTTGCAC AGTCCATTCA AGACTGGATC 
ATAAAAGAAG AAGCTATATA TCAGGAATCT AAAATGGCTG AGGAACTCAG CAGGAGCAAG 
CTTGAGCAGG AAGTGAAATC TCCTGCTAGT GGCAAAATTT CTTCCCCTAG CAAAATTTAT 
TCTGTTAAAA AATCTAAAAG TAACAAAGGA ATGAGCAAAA CAGAGTTAAT AGATCAAGAA 
AAAGAAAAAG AGAAAGAGAA GGAAAAGATT CCTTTCATTT TAGAAGGCTC TCTCAAGGCA 
TGGAAAGAAG AGCAAGATCG ATTAGCAGAA GAGGAACGCT TAAAGGAAGA AAAAAAAGCA 
GAGAAGAAGA GTAAGGACAT TGGTAAAAAG AAAGGCAAGG AAAAGGTAGA GAGAGAAGAT 
AGCAGGGTTT TGAAGAAAAA ATCTTTTTTC AAGGATAAAG CTAAAGAAGA GCAAATCAAG 
ATCTCAGACG TAACAGAGGA GCCCCTTCAA CAGCCAGAAC CTGAGATAGT TTACCCGTTT 
CGCGGATACA ATATGGGAGA TATACCCATC CAAATCTCGG GAACAAATTG TTATCTGTAT 
CCTTCAGATG GAGGGCAGAT TGAAGTAGAA AAGACAACAT TTGAAAAAGG TCCAACTTTT 
ATCAAAGTGA AAGTGAAAAA GGACAAGCAC AATTTTATAA TTCATTTAAA AGATCCTAAG 
AAAATTGTGA GAAACGAAAA AAAAGAAGAC GATTATTCTT CAGAAGATGA GAAAGATGAA 
GGAGAGGAAG AACGAAATCT TGAAATGAAA GAATCAGATG CAGAGAATAA AGCTATCAGC 
AAGTTTGGAT CTTTTTCTGC CACCTTGGAA AATGGAATCT GCCTCTCAAT AAGTTACTAC 
GGATCAAGTG GAATGGCGCC AGAGGATAAG GATCCTAATT TGGAAGCAAT GTTGAATATC 
CCGTCAGCAC TCACTCCAAC AATAATTCCT GTGGTAGTGA CTGTTCCTCA AGGCAAAGGA 
AAAGGGAAAA CCAAAGGCAA AGAAAAGCCC AAAGACTCTA TTAAAGAAGA GGAACATCCA 
AAAGAAGAAG AGAAAAAGGA AGAAGTAGAA CCAGAACCTG TTTTACAAGA GACTGTTTAT 
GTTCCCACCT TCCAAAACCT AAATGTGTCT TGCCCCAGTG GGCTTCTAGT GACCTTCATT 
GGCCAAGAGT CTGCAGATCA CCATGTTATG GATGAGGAAC CCACTTGGGA CATCATGGTC 
CGACAGAGCT ACCCCCAGAA GGTGAAGCAC TATGAGTTCT ACAAAACGGT GATGCCACCA 
GTGGAGCAGG AGGCATCGAG AGTTATCACT AGTCAAGGCA CTGTTGTCAA ATACATGTTG 
GATGGATCCA CACAGATTCT CTTTGCAAAT GGCGCTGTGA GCAGTAGTCC CGATTCAGGT 
CCTGTTTGTC CTCCTCCTGA AATGCCAGCG AGCCCTCATA ACGGAGACTT GATGGACAGT 
GTTTCTCAGC AAAAATCAGA AACAGCACCG TCTGAGACTG TCATCACAAA GAAAGGAAAA 
GGTCACAAAA ATCAGTCATC AGTGCCACAT ACGAATGAAA TCCATGAACC TCCCCAAGAG 
GTAGTTCAAA TAGTAACACC TGTGGAGGTT CATGTAGGCA CCTGGTTTAC AACTACACCT 
GACGGAAATC GGATTGGCAC CAAAGGATTA GAAAAAATAG CAGACTTGGC CCCATTCTTA 
TCCTTTCAGG CCACAGATCC TGTCAATGGA ACGGTTATGA CAACTCGAGA GGACAAGGTG 
ATCATTGTTG AAAAGAAGGA TGGTACTCGG ATCGTGGATC ATGCCGATGG TACCAGAATC 
ACAACCTTTT ATCAAGTTTA TGAAGATCAT ATTATGCCTC CAGATGATCA AGAAATACCC 
GAACATCCTC AAACTCTCAC CAGGCAGGTG AAGTGCATGC GCATAGAAAG CTCACACTAT 
GCCACTGTCA TCACCAACTG CGAGGACAGT AGCTGCTGTG CCACTTTTGG AGATGGGACG 
TCTATTATTG CAAAGCCACA GGGAACATAC CAGGTGTTAC CTTCAAACAA AGGCTGTCTT 
CATATTGATA GAGACTGTTC AGCTGTATAT TACCATGAAT CCAGCAGTGA TATATACCAT 
CCTTTCCAAA AACGTGAGCA GCTGAGAGCT AGCAGGTACA TCATGAAGCA CACGTCAGAG 
GTTCTCTGTG AAGTCCTGGA TCCTGAGGGA AACACCTTTC AGGTCATGGC TGATGGTAGC 
ACATCAACTC TGCTACCTGA AAACAATTTG GAAGATGATT TAAACTCAAA ACCTGAAGGC 
TATGATAGTT TATCATCTAC TCACCTTCAT AAAAATCACC TACAAATATA TGGTGAACAT 
GTCCCCAGGT TTTTTGTTAT CTATGCCGAT GGATCAGGAG TAGAACTTCT TCGAGATGAT 
GACATAGAAG AATACTTATC CTTGGCATAT GGAGAATCAA CCACTGTAGT TCTTCAAGAG 
CCAGTACAAG AACAGCCAGG CGCCCTGAGC ATCACTGTTC TTCGCCCTTT TCATGAAGCA 
TCGCCATGGC TAATGAAGAA AGAATTAGAC ACAATTGTTC CTCCTAATCT CCAGTCAAGG 
TCATGGGAGA AATTTCCCTC AGTTGAGAAA AAAACTCCAG GACCTCCCTT TGGCACTCAT 
ATTTGGAAGG GCCTTAACAT TGAGTGCAAA CAGCTAGTGA GTGCCCCAGC TCCTATACTC 
AAAAGCCCCA ATGTACTACA GATACGCCAG TTCATCCAGC ATGAGGTCAT AAACGATGAG 
GTGAAACTGA AGCTGCAGAT TTCCCTTAAG GACTATATAA ACCATATTTT AAAGAAAGAA 
GATGAGCTAC AGGAAATGAC AGTTAAAGAT TCCAGAACTG AGGAGGAGAG AGGCAATGCT 
GCAGATCTCC TCAAGCTTGT TATGTCTTTT CCTAAAATGG AGGAAACTAC AAAAGGTCAT 
GCTACTGAAG TTGCAGCCCA CTTAGTTGAT TTATTCAAGC AATCTTTGGC TGTTGCTCCA 
GAATGTCCAC CAGAAACATT TGATACAGAT TTCTTGGAAA AGAAAGGGAG GCACATAGCA 
AGTGAAAAGC GCTGGAAAGA AAAGATAGAT CAAAAGAGGA AGGAAATCGA AAAAACACAC 
AATTATCTAG AGGAAATCAG GAACAGAACG GTATCACCCT ACTTCAAATC AGAATTGAGC 
AAGACCTTTC AGTCTCAGTT TAATTATCTA GACGCCCTTT CCAAAAACTT GCCTCCTTTT 
ACAAAGAAAG TTGAAGATAC AAACAAAACA GCTGTTCAAA ATACATCCAA TCTTCAGCCA 
GATTCCCAGC CAAATAAAGT TTCACAACAA GAATCCTCAG ACACACTTAG TCTTCCAAAA 
TCAGAGGCTT TTGTGGATAT GAAGGCGTTC GCTAGCCAGA ACCAAACTGA AAATTTAACA 
AAATCTACTG AAGAACCAGA AACTTATGCC CCTGTGAAAA TTCCAACCCA GTCATTGCTG 
CAGGATGTTG CAGGACAAGC AAGGAAAGAG AAAGTGAGGT TACCTCGCTA TTTGCTGAGT 
TCCAAACCCA AGTCTCAACC TCTCGAGAAG GTGCAAGATT CTGTTGGAGG AAGAGTGAGA 
ACATCCTCGG TTGCTTCTGC TGCCATTAAT AACGCAAATT CATCCCCTTT TGGATTTCAT 
CTTCTCCCAC CGTCAGTGAA TTTTGGAGTG CTTGAAGAAG GGCACACCTA TGAAACCACT 
GTGAAGCTCA AGAATGTTGG AGTGGACTTC TGCAGGTTTA GGGTGAAGCA GCCGCCACCC 
AGCACAGGAT TGAAAGTGAC TTACAAACCT GGGCCTGTGG CAGCTGGTTT GCAGGCAGAA 
CTGAAAGTGG AGTTATTTAC CATGGTTGTT GGAGAGGATG GTGCCAAGGG ATCAGCACAC 
ATCTCTCACA ATATTGAGAT TATGACTGAG CATGATGTCC TGTTCTTACC TGTGGAAGCA 
ACAGTTTTAA CAAGCAGCAA TTATGATAAG CGATTAAAAG ATTTTCCCCA GGAAAAAGAA 
AACCCCACGA TCCAGAGGAC TTCTACAATT TCTTCTGCTA AACTTGGAGT CTTCATGTCT 
CGTAAAGTTT CTCCTTATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_001500885.3 ) cDNA ORF clone, Equus caballus(horse) -> XP_001500935.1 Equus caballus sperm associated antigen 17 (SPAG17), mRNA.

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CloneID	OHb21198
Clone ID Related Accession (Same CDS sequence)	XM_001500885.4 , XM_001500885.3
Accession Version	XM_001500885.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1447862400000
Organism	Equus caballus(horse)
Product	sperm-associated antigen 17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867420.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_001500885.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCA AGAAAGAGAA AAGCGGGTCG GCGAGCGCGA ATTCTAAGAT ATGGGAACCC 
TCGCTCATCG CTGCACACTT CAATCAGAAC GATTGGAAGG GCGTCATCGC TTTTGTAGTT 
GGAAACCGGA TTGAGGATGA GCTTCTCATT GGAGCCCTCA CCCTCGCTGT CCAGGCCCCT 
CAGCGTAGAC TCTTCAGTAT GGTATCATGG CAAGACATCC TCCAGCAGAT CAATGGAACA 
AATGCGCTTC CTGGAACTGC ATCTAAAAAG GGAAAGAAAC CTCTAGGCGG TAGCGCTCCC 
TTATATTCTG AGGTGTTAAT AGCAACAAAG GCAATTATGG ACAGTGGAGA GAAATTAACC 
TTACCGCTGA TAGGGAAACT CTTGAAATAT CAGCTTCTCC AGATTAAATA TAAGGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGGTGACA GAAGACAAAT TTAAGACTGA AAAGGATAAA 
GGGAAAGCGA AATCTCCCAA GGAAAAGAAG CCCTCAGGTA CCAAGGTTGT CAAAGGAAAG 
GGAAAGGATC AGCCTGAGGC AAACACAACA GTGAAAAAGG CCACTCAGTT AAAACAGAGG 
GGAGAAGAAG ACGACGCCAA ACGTTACATT GATGATGAGC CAGATGATGG TGCCCAACAT 
TACATTATAG TTGTGGGCTT CAACGATCCT CAGCTATTAG CGATTATGAC TGAGCTTGGC 
ATTCCCATAA GCAGCGTGAT TAAAATATCT TCAGAGAATT ATGAACCTCT GCAGGCGCAC 
CTGGCAGCAG TTAGCCAGCA GCAGGAAGTT TCTCTTCAGC CAGAAGATAT AGAAGCTGAA 
AAATTGAAGA ATGAGAATAC CATAAAAGAA CTTGAAATTT TCTGGAAGTA CTTGGAACCA 
ATCCTGAATA ATGAGAAACC TGAAACAAAT CTCTTTGATG TTGCTCGTCT TGAATACATG 
GTCAAGGAAG ATGGCTTTCC TTCTGACTGG TCAGACAGTG GGATGATGCT GGAATTGGGC 
ACCGACATTT TTGAAAATAT TGCCTGTTTG ATGTACGACA GCCTGGATTG GAAAAGGCAG 
CACCGGCACT ATTTAGAAAG CATGCAGCTT ATTAATGTCC CACAAGTGGT TAACGAGAAG 
CCTATCTTAG AAGCTGTAGC CATTGCTGAG GCTCCACAAG CTGCTGCTCC AACCCCTGGG 
AAGAAGAAAG CACAAAATGA AGAACGGCAA GCCCCAACAT CAGTGCCTTT TGTCATCACA 
ACTGAAGCAG ACATGAGATA TTACAATGAT TTGCTGAATC CGATTCCAGA AGAATTCATT 
TCTGTGCCCC TGATACTGCA CTGTATGCTG GAACAGGTTG TAGCAACTGA AGAAGACCTT 
GTCCCACCCA GTCTGGTGGA GCCGCCTCCC AGAGCAGATG GGTTGGACCA CAGAATTGCA 
GCTCACATTG TATCCATCCT ACCCTCTCTC TGTCTCACGG AGAAGGAGAA AAAGAATCTC 
CAGGAAATAT TTTTGTCTGA AGAAGAAAAT GAAAGCAAAG CAGTGCCCAA AGGTCCTCTC 
CTACTGAACT ATCACGACAC TCTGGCCCAC AAGAAGTATG CACTGAAGGA CCCAAAGAAT 
TTCGACCCAG TTCAAATTGA GCAGGAGATG CAGTCCAAGT TGCCACTGTG GGAATTCCTT 
GAATTCCCTC TGCCCCCGCC ATGGAACAGC ACTAAACGTC TAGCTACAAT TCATGAGCTC 
ATGCACTTTT GTACAAATGA AGTCTTGAGC TGGAATGAAG TAGAACGAGC CTTCAAAGTG 
TTTACTTTTG AGAGCCTGAA ACTCTCTACA GTTGATGAAA AAGGGAAACT AAAGCCTGCT 
CGGATGATGT ATGGGACTGA TTGTGAGATG TTCAACATAC CATGGGACAA CCCTGCCAGA 
TTTGCTAAAC AGATAAGGCA ACAATACATC ACGAAAATGA ACACCCAAGA GGTCAAACAG 
AAAACAGATA CTGAAACCAA AGACAGAATT TTGTTTGTGG ATCAGAATAT GCCAGTGTCT 
GTGCAAGATG ATGAGAGCAG TAAAGAACCT TCAGACCCTA GTCAGGCTGA TGCTAGCAAT 
ATGCAGCATT CTGACTCGAA TAATAAGAAA TCCTTAGACC CTGATGATAG AGAGCTGTCA 
GAGCAGGACA ACAGCTTGAA GACTCAGCTC CAATCTGAGC CTTTGGAGCA GACCACGGGC 
AATGAGATCA AAGATGAAGC AGTCACAAAT GTTGATCCTC CTGAAAAGAA ATCCAAGAAG 
ATGACCGTGG AAGCAGAGTT GGAGGACATA AAGAAAACAG AGCAACGCAG CCTAATGGAT 
TGGAGTTTCA CTGAACATTT TAAACCGAAA GTTCTACTGC AGGTTCTTCA AGAAGCCAAT 
CAGCATTACA GATGTATTGA TTCTTATTAC CACACCCAAG ACAACTCTTT ACTATTAGTC 
TTTCATAATC CAATGAATTT ACAACGTCTG CATTGTGAAT ATTGGAATGT TGCTCTTCAC 
TCCAATGTTG GATTCAGGAA TTATTTGGAA CTTGTTGCAC AGTCCATTCA AGACTGGATC 
ATAAAAGAAG AAGCTATATA TCAGGAATCT AAAATGGCTG AGGAACTCAG CAGGAGCAAG 
CTTGAGCAGG AAGTGAAATC TCCTGCTAGT GGCAAAATTT CTTCCCCTAG CAAAATTTAT 
TCTGTTAAAA AATCTAAAAG TAACAAAGGA ATGAGCAAAA CAGAGTTAAT AGATCAAGAA 
AAAGAAAAAG AGAAAGAGAA GGAAAAGATT CCTTTCATTT TAGAAGGCTC TCTCAAGGCA 
TGGAAAGAAG AGCAAGATCG ATTAGCAGAA GAGGAACGCT TAAAGGAAGA AAAAAAAGCA 
GAGAAGAAGA GTAAGGACAT TGGTAAAAAG AAAGGCAAGG AAAAGGTAGA GAGAGAAGAT 
AGCAGGGTTT TGAAGAAAAA ATCTTTTTTC AAGGATAAAG CTAAAGAAGA GCAAATCAAG 
ATCTCAGACG TAACAGAGGA GCCCCTTCAA CAGCCAGAAC CTGAGATAGT TTACCCGTTT 
CGCGGATACA ATATGGGAGA TATACCCATC CAAATCTCGG GAACAAATTG TTATCTGTAT 
CCTTCAGATG GAGGGCAGAT TGAAGTAGAA AAGACAACAT TTGAAAAAGG TCCAACTTTT 
ATCAAAGTGA AAGTGAAAAA GGACAAGCAC AATTTTATAA TTCATTTAAA AGATCCTAAG 
AAAATTGTGA GAAACGAAAA AAAAGAAGAC GATTATTCTT CAGAAGATGA GAAAGATGAA 
GGAGAGGAAG AACGAAATCT TGAAATGAAA GAATCAGATG CAGAGAATAA AGCTATCAGC 
AAGTTTGGAT CTTTTTCTGC CACCTTGGAA AATGGAATCT GCCTCTCAAT AAGTTACTAC 
GGATCAAGTG GAATGGCGCC AGAGGATAAG GATCCTAATT TGGAAGCAAT GTTGAATATC 
CCGTCAGCAC TCACTCCAAC AATAATTCCT GTGGTAGTGA CTGTTCCTCA AGGCAAAGGA 
AAAGGGAAAA CCAAAGGCAA AGAAAAGCCC AAAGACTCTA TTAAAGAAGA GGAACATCCA 
AAAGAAGAAG AGAAAAAGGA AGAAGTAGAA CCAGAACCTG TTTTACAAGA GACTGTTTAT 
GTTCCCACCT TCCAAAACCT AAATGTGTCT TGCCCCAGTG GGCTTCTAGT GACCTTCATT 
GGCCAAGAGT CTGCAGATCA CCATGTTATG GATGAGGAAC CCACTTGGGA CATCATGGTC 
CGACAGAGCT ACCCCCAGAA GGTGAAGCAC TATGAGTTCT ACAAAACGGT GATGCCACCA 
GTGGAGCAGG AGGCATCGAG AGTTATCACT AGTCAAGGCA CTGTTGTCAA ATACATGTTG 
GATGGATCCA CACAGATTCT CTTTGCAAAT GGCGCTGTGA GCAGTAGTCC CGATTCAGGT 
CCTGTTTGTC CTCCTCCTGA AATGCCAGCG AGCCCTCATA ACGGAGACTT GATGGACAGT 
GTTTCTCAGC AAAAATCAGA AACAGCACCG TCTGAGACTG TCATCACAAA GAAAGGAAAA 
GGTCACAAAA ATCAGTCATC AGTGCCACAT ACGAATGAAA TCCATGAACC TCCCCAAGAG 
GTAGTTCAAA TAGTAACACC TGTGGAGGTT CATGTAGGCA CCTGGTTTAC AACTACACCT 
GACGGAAATC GGATTGGCAC CAAAGGATTA GAAAAAATAG CAGACTTGGC CCCATTCTTA 
TCCTTTCAGG CCACAGATCC TGTCAATGGA ACGGTTATGA CAACTCGAGA GGACAAGGTG 
ATCATTGTTG AAAAGAAGGA TGGTACTCGG ATCGTGGATC ATGCCGATGG TACCAGAATC 
ACAACCTTTT ATCAAGTTTA TGAAGATCAT ATTATGCCTC CAGATGATCA AGAAATACCC 
GAACATCCTC AAACTCTCAC CAGGCAGGTG AAGTGCATGC GCATAGAAAG CTCACACTAT 
GCCACTGTCA TCACCAACTG CGAGGACAGT AGCTGCTGTG CCACTTTTGG AGATGGGACG 
TCTATTATTG CAAAGCCACA GGGAACATAC CAGGTGTTAC CTTCAAACAA AGGCTGTCTT 
CATATTGATA GAGACTGTTC AGCTGTATAT TACCATGAAT CCAGCAGTGA TATATACCAT 
CCTTTCCAAA AACGTGAGCA GCTGAGAGCT AGCAGGTACA TCATGAAGCA CACGTCAGAG 
GTTCTCTGTG AAGTCCTGGA TCCTGAGGGA AACACCTTTC AGGTCATGGC TGATGGTAGC 
ACATCAACTC TGCTACCTGA AAACAATTTG GAAGATGATT TAAACTCAAA ACCTGAAGGC 
TATGATAGTT TATCATCTAC TCACCTTCAT AAAAATCACC TACAAATATA TGGTGAACAT 
GTCCCCAGGT TTTTTGTTAT CTATGCCGAT GGATCAGGAG TAGAACTTCT TCGAGATGAT 
GACATAGAAG AATACTTATC CTTGGCATAT GGAGAATCAA CCACTGTAGT TCTTCAAGAG 
CCAGTACAAG AACAGCCAGG CGCCCTGAGC ATCACTGTTC TTCGCCCTTT TCATGAAGCA 
TCGCCATGGC TAATGAAGAA AGAATTAGAC ACAATTGTTC CTCCTAATCT CCAGTCAAGG 
TCATGGGAGA AATTTCCCTC AGTTGAGAAA AAAACTCCAG GACCTCCCTT TGGCACTCAT 
ATTTGGAAGG GCCTTAACAT TGAGTGCAAA CAGCTAGTGA GTGCCCCAGC TCCTATACTC 
AAAAGCCCCA ATGTACTACA GATACGCCAG TTCATCCAGC ATGAGGTCAT AAACGATGAG 
GTGAAACTGA AGCTGCAGAT TTCCCTTAAG GACTATATAA ACCATATTTT AAAGAAAGAA 
GATGAGCTAC AGGAAATGAC AGTTAAAGAT TCCAGAACTG AGGAGGAGAG AGGCAATGCT 
GCAGATCTCC TCAAGCTTGT TATGTCTTTT CCTAAAATGG AGGAAACTAC AAAAGGTCAT 
GCTACTGAAG TTGCAGCCCA CTTAGTTGAT TTATTCAAGC AATCTTTGGC TGTTGCTCCA 
GAATGTCCAC CAGAAACATT TGATACAGAT TTCTTGGAAA AGAAAGGGAG GCACATAGCA 
AGTGAAAAGC GCTGGAAAGA AAAGATAGAT CAAAAGAGGA AGGAAATCGA AAAAACACAC 
AATTATCTAG AGGAAATCAG GAACAGAACG GTATCACCCT ACTTCAAATC AGAATTGAGC 
AAGACCTTTC AGTCTCAGTT TAATTATCTA GACGCCCTTT CCAAAAACTT GCCTCCTTTT 
ACAAAGAAAG TTGAAGATAC AAACAAAACA GCTGTTCAAA ATACATCCAA TCTTCAGCCA 
GATTCCCAGC CAAATAAAGT TTCACAACAA GAATCCTCAG ACACACTTAG TCTTCCAAAA 
TCAGAGGCTT TTGTGGATAT GAAGGCGTTC GCTAGCCAGA ACCAAACTGA AAATTTAACA 
AAATCTACTG AAGAACCAGA AACTTATGCC CCTGTGAAAA TTCCAACCCA GTCATTGCTG 
CAGGATGTTG CAGGACAAGC AAGGAAAGAG AAAGTGAGGT TACCTCGCTA TTTGCTGAGT 
TCCAAACCCA AGTCTCAACC TCTCGAGAAG GTGCAAGATT CTGTTGGAGG AAGAGTGAGA 
ACATCCTCGG TTGCTTCTGC TGCCATTAAT AACGCAAATT CATCCCCTTT TGGATTTCAT 
CTTCTCCCAC CGTCAGTGAA TTTTGGAGTG CTTGAAGAAG GGCACACCTA TGAAACCACT 
GTGAAGCTCA AGAATGTTGG AGTGGACTTC TGCAGGTTTA GGGTGAAGCA GCCGCCACCC 
AGCACAGGAT TGAAAGTGAC TTACAAACCT GGGCCTGTGG CAGCTGGTTT GCAGGCAGAA 
CTGAAAGTGG AGTTATTTAC CATGGTTGTT GGAGAGGATG GTGCCAAGGG ATCAGCACAC 
ATCTCTCACA ATATTGAGAT TATGACTGAG CATGATGTCC TGTTCTTACC TGTGGAAGCA 
ACAGTTTTAA CAAGCAGCAA TTATGATAAG CGATTAAAAG ATTTTCCCCA GGAAAAAGAA 
AACCCCACGA TCCAGAGGAC TTCTACAATT TCTTCTGCTA AACTTGGAGT CTTCATGTCT 
CGTAAAGTTT CTCCTTATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001500935.1
CDS	69..6749
Translation	MAPKKEKSGSASANSKIWEPSLIAAHFNQNDWKGVIAFVVGNRIEDELLIGALTLAVQAPQRRLFSMVSWQDILQQINGTNALPGTASKKGKKPLGGSAPLYSEVLIATKAIMDSGEKLTLPLIGKLLKYQLLQIKYKDQQRRESEKKVTEDKFKTEKDKGKAKSPKEKKPSGTKVVKGKGKDQPEANTTVKKATQLKQRGEEDDAKRYIDDEPDDGAQHYIIVVGFNDPQLLAIMTELGIPISSVIKISSENYEPLQAHLAAVSQQQEVSLQPEDIEAEKLKNENTIKELEIFWKYLEPILNNEKPETNLFDVARLEYMVKEDGFPSDWSDSGMMLELGTDIFENIACLMYDSLDWKRQHRHYLESMQLINVPQVVNEKPILEAVAIAEAPQAAAPTPGKKKAQNEERQAPTSVPFVITTEADMRYYNDLLNPIPEEFISVPLILHCMLEQVVATEEDLVPPSLVEPPPRADGLDHRIAAHIVSILPSLCLTEKEKKNLQEIFLSEEENESKAVPKGPLLLNYHDTLAHKKYALKDPKNFDPVQIEQEMQSKLPLWEFLEFPLPPPWNSTKRLATIHELMHFCTNEVLSWNEVERAFKVFTFESLKLSTVDEKGKLKPARMMYGTDCEMFNIPWDNPARFAKQIRQQYITKMNTQEVKQKTDTETKDRILFVDQNMPVSVQDDESSKEPSDPSQADASNMQHSDSNNKKSLDPDDRELSEQDNSLKTQLQSEPLEQTTGNEIKDEAVTNVDPPEKKSKKMTVEAELEDIKKTEQRSLMDWSFTEHFKPKVLLQVLQEANQHYRCIDSYYHTQDNSLLLVFHNPMNLQRLHCEYWNVALHSNVGFRNYLELVAQSIQDWIIKEEAIYQESKMAEELSRSKLEQEVKSPASGKISSPSKIYSVKKSKSNKGMSKTELIDQEKEKEKEKEKIPFILEGSLKAWKEEQDRLAEEERLKEEKKAEKKSKDIGKKKGKEKVEREDSRVLKKKSFFKDKAKEEQIKISDVTEEPLQQPEPEIVYPFRGYNMGDIPIQISGTNCYLYPSDGGQIEVEKTTFEKGPTFIKVKVKKDKHNFIIHLKDPKKIVRNEKKEDDYSSEDEKDEGEEERNLEMKESDAENKAISKFGSFSATLENGICLSISYYGSSGMAPEDKDPNLEAMLNIPSALTPTIIPVVVTVPQGKGKGKTKGKEKPKDSIKEEEHPKEEEKKEEVEPEPVLQETVYVPTFQNLNVSCPSGLLVTFIGQESADHHVMDEEPTWDIMVRQSYPQKVKHYEFYKTVMPPVEQEASRVITSQGTVVKYMLDGSTQILFANGAVSSSPDSGPVCPPPEMPASPHNGDLMDSVSQQKSETAPSETVITKKGKGHKNQSSVPHTNEIHEPPQEVVQIVTPVEVHVGTWFTTTPDGNRIGTKGLEKIADLAPFLSFQATDPVNGTVMTTREDKVIIVEKKDGTRIVDHADGTRITTFYQVYEDHIMPPDDQEIPEHPQTLTRQVKCMRIESSHYATVITNCEDSSCCATFGDGTSIIAKPQGTYQVLPSNKGCLHIDRDCSAVYYHESSSDIYHPFQKREQLRASRYIMKHTSEVLCEVLDPEGNTFQVMADGSTSTLLPENNLEDDLNSKPEGYDSLSSTHLHKNHLQIYGEHVPRFFVIYADGSGVELLRDDDIEEYLSLAYGESTTVVLQEPVQEQPGALSITVLRPFHEASPWLMKKELDTIVPPNLQSRSWEKFPSVEKKTPGPPFGTHIWKGLNIECKQLVSAPAPILKSPNVLQIRQFIQHEVINDEVKLKLQISLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKGHATEVAAHLVDLFKQSLAVAPECPPETFDTDFLEKKGRHIASEKRWKEKIDQKRKEIEKTHNYLEEIRNRTVSPYFKSELSKTFQSQFNYLDALSKNLPPFTKKVEDTNKTAVQNTSNLQPDSQPNKVSQQESSDTLSLPKSEAFVDMKAFASQNQTENLTKSTEEPETYAPVKIPTQSLLQDVAGQARKEKVRLPRYLLSSKPKSQPLEKVQDSVGGRVRTSSVASAAINNANSSPFGFHLLPPSVNFGVLEEGHTYETTVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQAELKVELFTMVVGEDGAKGSAHISHNIEIMTEHDVLFLPVEATVLTSSNYDKRLKDFPQEKENPTIQRTSTISSAKLGVFMSRKVSPY

Target ORF information:

RefSeq Version	XM_001500885.3
Organism	Equus caballus(horse)
Definition	Equus caballus sperm associated antigen 17 (SPAG17), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001500885.3

ORF Insert Sequence:

ATGGCACCCA AGAAAGAGAA AAGCGGGTCG GCGAGCGCGA ATTCTAAGAT ATGGGAACCC 
TCGCTCATCG CTGCACACTT CAATCAGAAC GATTGGAAGG GCGTCATCGC TTTTGTAGTT 
GGAAACCGGA TTGAGGATGA GCTTCTCATT GGAGCCCTCA CCCTCGCTGT CCAGGCCCCT 
CAGCGTAGAC TCTTCAGTAT GGTATCATGG CAAGACATCC TCCAGCAGAT CAATGGAACA 
AATGCGCTTC CTGGAACTGC ATCTAAAAAG GGAAAGAAAC CTCTAGGCGG TAGCGCTCCC 
TTATATTCTG AGGTGTTAAT AGCAACAAAG GCAATTATGG ACAGTGGAGA GAAATTAACC 
TTACCGCTGA TAGGGAAACT CTTGAAATAT CAGCTTCTCC AGATTAAATA TAAGGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGGTGACA GAAGACAAAT TTAAGACTGA AAAGGATAAA 
GGGAAAGCGA AATCTCCCAA GGAAAAGAAG CCCTCAGGTA CCAAGGTTGT CAAAGGAAAG 
GGAAAGGATC AGCCTGAGGC AAACACAACA GTGAAAAAGG CCACTCAGTT AAAACAGAGG 
GGAGAAGAAG ACGACGCCAA ACGTTACATT GATGATGAGC CAGATGATGG TGCCCAACAT 
TACATTATAG TTGTGGGCTT CAACGATCCT CAGCTATTAG CGATTATGAC TGAGCTTGGC 
ATTCCCATAA GCAGCGTGAT TAAAATATCT TCAGAGAATT ATGAACCTCT GCAGGCGCAC 
CTGGCAGCAG TTAGCCAGCA GCAGGAAGTT TCTCTTCAGC CAGAAGATAT AGAAGCTGAA 
AAATTGAAGA ATGAGAATAC CATAAAAGAA CTTGAAATTT TCTGGAAGTA CTTGGAACCA 
ATCCTGAATA ATGAGAAACC TGAAACAAAT CTCTTTGATG TTGCTCGTCT TGAATACATG 
GTCAAGGAAG ATGGCTTTCC TTCTGACTGG TCAGACAGTG GGATGATGCT GGAATTGGGC 
ACCGACATTT TTGAAAATAT TGCCTGTTTG ATGTACGACA GCCTGGATTG GAAAAGGCAG 
CACCGGCACT ATTTAGAAAG CATGCAGCTT ATTAATGTCC CACAAGTGGT TAACGAGAAG 
CCTATCTTAG AAGCTGTAGC CATTGCTGAG GCTCCACAAG CTGCTGCTCC AACCCCTGGG 
AAGAAGAAAG CACAAAATGA AGAACGGCAA GCCCCAACAT CAGTGCCTTT TGTCATCACA 
ACTGAAGCAG ACATGAGATA TTACAATGAT TTGCTGAATC CGATTCCAGA AGAATTCATT 
TCTGTGCCCC TGATACTGCA CTGTATGCTG GAACAGGTTG TAGCAACTGA AGAAGACCTT 
GTCCCACCCA GTCTGGTGGA GCCGCCTCCC AGAGCAGATG GGTTGGACCA CAGAATTGCA 
GCTCACATTG TATCCATCCT ACCCTCTCTC TGTCTCACGG AGAAGGAGAA AAAGAATCTC 
CAGGAAATAT TTTTGTCTGA AGAAGAAAAT GAAAGCAAAG CAGTGCCCAA AGGTCCTCTC 
CTACTGAACT ATCACGACAC TCTGGCCCAC AAGAAGTATG CACTGAAGGA CCCAAAGAAT 
TTCGACCCAG TTCAAATTGA GCAGGAGATG CAGTCCAAGT TGCCACTGTG GGAATTCCTT 
GAATTCCCTC TGCCCCCGCC ATGGAACAGC ACTAAACGTC TAGCTACAAT TCATGAGCTC 
ATGCACTTTT GTACAAATGA AGTCTTGAGC TGGAATGAAG TAGAACGAGC CTTCAAAGTG 
TTTACTTTTG AGAGCCTGAA ACTCTCTACA GTTGATGAAA AAGGGAAACT AAAGCCTGCT 
CGGATGATGT ATGGGACTGA TTGTGAGATG TTCAACATAC CATGGGACAA CCCTGCCAGA 
TTTGCTAAAC AGATAAGGCA ACAATACATC ACGAAAATGA ACACCCAAGA GGTCAAACAG 
AAAACAGATA CTGAAACCAA AGACAGAATT TTGTTTGTGG ATCAGAATAT GCCAGTGTCT 
GTGCAAGATG ATGAGAGCAG TAAAGAACCT TCAGACCCTA GTCAGGCTGA TGCTAGCAAT 
ATGCAGCATT CTGACTCGAA TAATAAGAAA TCCTTAGACC CTGATGATAG AGAGCTGTCA 
GAGCAGGACA ACAGCTTGAA GACTCAGCTC CAATCTGAGC CTTTGGAGCA GACCACGGGC 
AATGAGATCA AAGATGAAGC AGTCACAAAT GTTGATCCTC CTGAAAAGAA ATCCAAGAAG 
ATGACCGTGG AAGCAGAGTT GGAGGACATA AAGAAAACAG AGCAACGCAG CCTAATGGAT 
TGGAGTTTCA CTGAACATTT TAAACCGAAA GTTCTACTGC AGGTTCTTCA AGAAGCCAAT 
CAGCATTACA GATGTATTGA TTCTTATTAC CACACCCAAG ACAACTCTTT ACTATTAGTC 
TTTCATAATC CAATGAATTT ACAACGTCTG CATTGTGAAT ATTGGAATGT TGCTCTTCAC 
TCCAATGTTG GATTCAGGAA TTATTTGGAA CTTGTTGCAC AGTCCATTCA AGACTGGATC 
ATAAAAGAAG AAGCTATATA TCAGGAATCT AAAATGGCTG AGGAACTCAG CAGGAGCAAG 
CTTGAGCAGG AAGTGAAATC TCCTGCTAGT GGCAAAATTT CTTCCCCTAG CAAAATTTAT 
TCTGTTAAAA AATCTAAAAG TAACAAAGGA ATGAGCAAAA CAGAGTTAAT AGATCAAGAA 
AAAGAAAAAG AGAAAGAGAA GGAAAAGATT CCTTTCATTT TAGAAGGCTC TCTCAAGGCA 
TGGAAAGAAG AGCAAGATCG ATTAGCAGAA GAGGAACGCT TAAAGGAAGA AAAAAAAGCA 
GAGAAGAAGA GTAAGGACAT TGGTAAAAAG AAAGGCAAGG AAAAGGTAGA GAGAGAAGAT 
AGCAGGGTTT TGAAGAAAAA ATCTTTTTTC AAGGATAAAG CTAAAGAAGA GCAAATCAAG 
ATCTCAGACG TAACAGAGGA GCCCCTTCAA CAGCCAGAAC CTGAGATAGT TTACCCGTTT 
CGCGGATACA ATATGGGAGA TATACCCATC CAAATCTCGG GAACAAATTG TTATCTGTAT 
CCTTCAGATG GAGGGCAGAT TGAAGTAGAA AAGACAACAT TTGAAAAAGG TCCAACTTTT 
ATCAAAGTGA AAGTGAAAAA GGACAAGCAC AATTTTATAA TTCATTTAAA AGATCCTAAG 
AAAATTGTGA GAAACGAAAA AAAAGAAGAC GATTATTCTT CAGAAGATGA GAAAGATGAA 
GGAGAGGAAG AACGAAATCT TGAAATGAAA GAATCAGATG CAGAGAATAA AGCTATCAGC 
AAGTTTGGAT CTTTTTCTGC CACCTTGGAA AATGGAATCT GCCTCTCAAT AAGTTACTAC 
GGATCAAGTG GAATGGCGCC AGAGGATAAG GATCCTAATT TGGAAGCAAT GTTGAATATC 
CCGTCAGCAC TCACTCCAAC AATAATTCCT GTGGTAGTGA CTGTTCCTCA AGGCAAAGGA 
AAAGGGAAAA CCAAAGGCAA AGAAAAGCCC AAAGACTCTA TTAAAGAAGA GGAACATCCA 
AAAGAAGAAG AGAAAAAGGA AGAAGTAGAA CCAGAACCTG TTTTACAAGA GACTGTTTAT 
GTTCCCACCT TCCAAAACCT AAATGTGTCT TGCCCCAGTG GGCTTCTAGT GACCTTCATT 
GGCCAAGAGT CTGCAGATCA CCATGTTATG GATGAGGAAC CCACTTGGGA CATCATGGTC 
CGACAGAGCT ACCCCCAGAA GGTGAAGCAC TATGAGTTCT ACAAAACGGT GATGCCACCA 
GTGGAGCAGG AGGCATCGAG AGTTATCACT AGTCAAGGCA CTGTTGTCAA ATACATGTTG 
GATGGATCCA CACAGATTCT CTTTGCAAAT GGCGCTGTGA GCAGTAGTCC CGATTCAGGT 
CCTGTTTGTC CTCCTCCTGA AATGCCAGCG AGCCCTCATA ACGGAGACTT GATGGACAGT 
GTTTCTCAGC AAAAATCAGA AACAGCACCG TCTGAGACTG TCATCACAAA GAAAGGAAAA 
GGTCACAAAA ATCAGTCATC AGTGCCACAT ACGAATGAAA TCCATGAACC TCCCCAAGAG 
GTAGTTCAAA TAGTAACACC TGTGGAGGTT CATGTAGGCA CCTGGTTTAC AACTACACCT 
GACGGAAATC GGATTGGCAC CAAAGGATTA GAAAAAATAG CAGACTTGGC CCCATTCTTA 
TCCTTTCAGG CCACAGATCC TGTCAATGGA ACGGTTATGA CAACTCGAGA GGACAAGGTG 
ATCATTGTTG AAAAGAAGGA TGGTACTCGG ATCGTGGATC ATGCCGATGG TACCAGAATC 
ACAACCTTTT ATCAAGTTTA TGAAGATCAT ATTATGCCTC CAGATGATCA AGAAATACCC 
GAACATCCTC AAACTCTCAC CAGGCAGGTG AAGTGCATGC GCATAGAAAG CTCACACTAT 
GCCACTGTCA TCACCAACTG CGAGGACAGT AGCTGCTGTG CCACTTTTGG AGATGGGACG 
TCTATTATTG CAAAGCCACA GGGAACATAC CAGGTGTTAC CTTCAAACAA AGGCTGTCTT 
CATATTGATA GAGACTGTTC AGCTGTATAT TACCATGAAT CCAGCAGTGA TATATACCAT 
CCTTTCCAAA AACGTGAGCA GCTGAGAGCT AGCAGGTACA TCATGAAGCA CACGTCAGAG 
GTTCTCTGTG AAGTCCTGGA TCCTGAGGGA AACACCTTTC AGGTCATGGC TGATGGTAGC 
ACATCAACTC TGCTACCTGA AAACAATTTG GAAGATGATT TAAACTCAAA ACCTGAAGGC 
TATGATAGTT TATCATCTAC TCACCTTCAT AAAAATCACC TACAAATATA TGGTGAACAT 
GTCCCCAGGT TTTTTGTTAT CTATGCCGAT GGATCAGGAG TAGAACTTCT TCGAGATGAT 
GACATAGAAG AATACTTATC CTTGGCATAT GGAGAATCAA CCACTGTAGT TCTTCAAGAG 
CCAGTACAAG AACAGCCAGG CGCCCTGAGC ATCACTGTTC TTCGCCCTTT TCATGAAGCA 
TCGCCATGGC TAATGAAGAA AGAATTAGAC ACAATTGTTC CTCCTAATCT CCAGTCAAGG 
TCATGGGAGA AATTTCCCTC AGTTGAGAAA AAAACTCCAG GACCTCCCTT TGGCACTCAT 
ATTTGGAAGG GCCTTAACAT TGAGTGCAAA CAGCTAGTGA GTGCCCCAGC TCCTATACTC 
AAAAGCCCCA ATGTACTACA GATACGCCAG TTCATCCAGC ATGAGGTCAT AAACGATGAG 
GTGAAACTGA AGCTGCAGAT TTCCCTTAAG GACTATATAA ACCATATTTT AAAGAAAGAA 
GATGAGCTAC AGGAAATGAC AGTTAAAGAT TCCAGAACTG AGGAGGAGAG AGGCAATGCT 
GCAGATCTCC TCAAGCTTGT TATGTCTTTT CCTAAAATGG AGGAAACTAC AAAAGGTCAT 
GCTACTGAAG TTGCAGCCCA CTTAGTTGAT TTATTCAAGC AATCTTTGGC TGTTGCTCCA 
GAATGTCCAC CAGAAACATT TGATACAGAT TTCTTGGAAA AGAAAGGGAG GCACATAGCA 
AGTGAAAAGC GCTGGAAAGA AAAGATAGAT CAAAAGAGGA AGGAAATCGA AAAAACACAC 
AATTATCTAG AGGAAATCAG GAACAGAACG GTATCACCCT ACTTCAAATC AGAATTGAGC 
AAGACCTTTC AGTCTCAGTT TAATTATCTA GACGCCCTTT CCAAAAACTT GCCTCCTTTT 
ACAAAGAAAG TTGAAGATAC AAACAAAACA GCTGTTCAAA ATACATCCAA TCTTCAGCCA 
GATTCCCAGC CAAATAAAGT TTCACAACAA GAATCCTCAG ACACACTTAG TCTTCCAAAA 
TCAGAGGCTT TTGTGGATAT GAAGGCGTTC GCTAGCCAGA ACCAAACTGA AAATTTAACA 
AAATCTACTG AAGAACCAGA AACTTATGCC CCTGTGAAAA TTCCAACCCA GTCATTGCTG 
CAGGATGTTG CAGGACAAGC AAGGAAAGAG AAAGTGAGGT TACCTCGCTA TTTGCTGAGT 
TCCAAACCCA AGTCTCAACC TCTCGAGAAG GTGCAAGATT CTGTTGGAGG AAGAGTGAGA 
ACATCCTCGG TTGCTTCTGC TGCCATTAAT AACGCAAATT CATCCCCTTT TGGATTTCAT 
CTTCTCCCAC CGTCAGTGAA TTTTGGAGTG CTTGAAGAAG GGCACACCTA TGAAACCACT 
GTGAAGCTCA AGAATGTTGG AGTGGACTTC TGCAGGTTTA GGGTGAAGCA GCCGCCACCC 
AGCACAGGAT TGAAAGTGAC TTACAAACCT GGGCCTGTGG CAGCTGGTTT GCAGGCAGAA 
CTGAAAGTGG AGTTATTTAC CATGGTTGTT GGAGAGGATG GTGCCAAGGG ATCAGCACAC 
ATCTCTCACA ATATTGAGAT TATGACTGAG CATGATGTCC TGTTCTTACC TGTGGAAGCA 
ACAGTTTTAA CAAGCAGCAA TTATGATAAG CGATTAAAAG ATTTTCCCCA GGAAAAAGAA 
AACCCCACGA TCCAGAGGAC TTCTACAATT TCTTCTGCTA AACTTGGAGT CTTCATGTCT 
CGTAAAGTTT CTCCTTATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_023641329.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023497097.1 Equus caballus sperm associated antigen 17 (SPAG17), transcript variant X2, mRNA.

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CloneID	OHb65449
Clone ID Related Accession (Same CDS sequence)	XM_023641329.1
Accession Version	XM_023641329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	sperm-associated antigen 17 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCA AGAAAGAGAA AAGCGGGTCG GCGAGCGCGA ATTCTAAGAT ATGGGAACCC 
TCGCTCATCG CTGCACACTT CAATCAGAAC GATTGGAAGG GCGTCATCGC TTTTGTAGTT 
GGAAACCGGA TTGAGGATGA GCTTCTCATT GGAGCCCTCA CCCTCGCTGT CCAGGCCCCT 
CAGCGTAGAC TCTTCAGTAT GGTATCATGG CAAGACATCC TCCAGCAGAT CAATGGAACA 
AATGCGCTTC CTGGAACTGC ATCTAAAAAG GGAAAGAAAC CTCTAGGCGG TAGCGCTCCC 
TTATATTCTG AGGTGTTAAT AGCAACAAAG GCAATTATGG ACAGTGGAGA GAAATTAACC 
TTACCGCTGA TAGGGAAACT CTTGAAATAT CAGCTTCTCC AGATTAAATA TAAGGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGGTGACA GAAGACAAAT TTAAGACTGA AAAGGATAAA 
GGGAAAGCGA AATCTCCCAA GGAAAAGAAG CCCTCAGGTA CCAAGGTTGT CAAAGGAAAG 
GGAAAGGATC AGCCTGAGGC AAACACAACA GTGAAAAAGG CCACTCAGTT AAAACAGAGG 
GGAGAAGAAG ACGACGCCAA ACGTTACATT GATGATGAGC CAGATGATGG TGCCCAACAT 
TACATTATAG TTGTGGGCTT CAACGATCCT CAGCTATTAG CGATTATGAC TGAGCTTGGC 
ATTCCCATAA GCAGCGTGAT TAAAATATCT TCAGAGAATT ATGAACCTCT GCAGGCGCAC 
CTGGCAGCAG TTAGCCAGCA GCAGGAAGTT TCTCTTCAGC CAGAAGATAT AGAAGCTGAA 
AAATTGAAGA ATGAGAATAC CATAAAAGAA CTTGAAATTT TCTGGAAGTA CTTGGAACCA 
ATCCTGAATA ATGAGAAACC TGAAACAAAT CTCTTTGATG TTGCTCGTCT TGAATACATG 
GTCAAGGAAG ATGGCTTTCC TTCTGACTGG TCAGACAGTG GGATGATGCT GGAATTGGGC 
ACCGACATTT TTGAAAATAT TGCCTGTTTG ATGTACGACA GCCTGGATTG GAAAAGGCAG 
CACCGGCACT ATTTAGAAAG CATGCAGCTT ATTAATGTCC CACAAGTGGT TAACGAGAAG 
CCTATCTTAG AAGCTGTAGC CATTGCTGAG GCTCCACAAG CTGCTGCTCC AACCCCTGGG 
AAGAAGAAAG CACAAAATGA AGAACGGCAA GCCCCAACAT CAGTGCCTTT TGTCATCACA 
ACTGAAGCAG ACATGAGATA TTACAATGAT TTGCTGAATC CGATTCCAGA AGAATTCATT 
TCTGTGCCCC TGATACTGCA CTGTATGCTG GAACAGGTTG TAGCAACTGA AGAAGACCTT 
GTCCCACCCA GTCTGGTGGA GCCGCCTCCC AGAGCAGATG GGTTGGACCA CAGAATTGCA 
GCTCACATTG TATCCATCCT ACCCTCTCTC TGTCTCACGG AGAAGGAGAA AAAGAATCTC 
CAGGAAATAT TTTTGTCTGA AGAAGAAAAT GAAAGCAAAG CAGTGCCCAA AGGTCCTCTC 
CTACTGAACT ATCACGACAC TCTGGCCCAC AAGAAGTATG CACTGAAGGA CCCAAAGAAT 
TTCGACCCAG TTCAAATTGA GCAGGAGATG CAGTCCAAGT TGCCACTGTG GGAATTCCTT 
GAATTCCCTC TGCCCCCGCC ATGGAACAGC ACTAAACGTC TAGCTACAAT TCATGAGCTC 
ATGCACTTTT GTACAAATGA AGTCTTGAGC TGGAATGAAG TAGAACGAGC CTTCAAAGTG 
TTTACTTTTG AGAGCCTGAA ACTCTCTACA GTTGATGAAA AAGGGAAACT AAAGCCTGCT 
CGGATGATGT ATGGGACTGA TTGTGAGATG TTCAACATAC CATGGGACAA CCCTGCCAGA 
TTTGCTAAAC AGATAAGGCA ACAATACATC ACGAAAATGA ACACCCAAGA GGTCAAACAG 
AAAACAGATA CTGAAACCAA AGACAGAATT TTGTTTGTGG ATCAGAATAT GCCAGTGTCT 
GTGCAAGATG ATGAGAGCAG TAAAGAACCT TCAGACCCTA GTCAGGCTGA TGCTAGCAAT 
ATGCAGCATT CTGACTCGAA TAATAAGAAA TCCTTAGACC CTGATGATAG AGAGCTGTCA 
GAGCAGGACA ACAGCTTGAA GACTCAGCTC CAATCTGAGC CTTTGGAGCA GACCACGGGC 
AATGAGATCA AAGATGAAGC AGTCACAAAT GTTGATCCTC CTGAAAAGAA ATCCAAGAAG 
ATGACCGTGG AAGCAGAGTT GGAGGACATA AAGAAAACAG AGCAACGCAG CCTAATGGAT 
TGGAGTTTCA CTGAACATTT TAAACCGAAA GTTCTACTGC AGGTTCTTCA AGAAGCCAAT 
CAGCATTACA GATGTATTGA TTCTTATTAC CACACCCAAG ACAACTCTTT ACTATTAGTC 
TTTCATAATC CAATGAATTT ACAACGTCTG CATTGTGAAT ATTGGAATGT TGCTCTTCAC 
TCCAATGTTG GATTCAGGAA TTATTTGGAA CTTGTTGCAC AGTCCATTCA AGACTGGATC 
ATAAAAGAAG AAGCTATATA TCAGGAATCT AAAATGGCTG AGGAACTCAG CAGGAGCAAG 
CTTGAGCAGG AAGTGAAATC TCCTGCTAGT GGCAAAATTT CTTCCCCTAG CAAAATTTAT 
TCTGTTAAAA AATCTAAAAG TAACAAAGGA ATGAGCAAAA CAGAGTTAAT AGATCAAGAA 
AAAGAAAAAG AGAAAGAGAA GGAAAAGATT CCTTTCATTT TAGAAGGCTC TCTCAAGGCA 
TGGAAAGAAG AGCAAGATCG ATTAGCAGAA GAGGAACGCT TAAAGGAAGA AAAAAAAGCA 
GAGAAGAAGA GTAAGGACAT TGGTAAAAAG AAAGGCAAGG AAAAGGTAGA GAGAGAAGAT 
AGCAGGGTTT TGAAGAAAAA ATCTTTTTTC AAGGATAAAG CTAAAGAAGA GCAAATCAAG 
ATCTCAGACG TAACAGAGGA GCCCCTTCAA CAGCCAGAAC CTGAGATAGT TTACCCGTTT 
CGCGGATACA ATATGGGAGA TATACCCATC CAAATCTCGG GAACAAATTG TTATCTGTAT 
CCTTCAGATG GAGGGCAGAT TGAAGTAGAA AAGACAACAT TTGAAAAAGG TCCAACTTTT 
ATCAAAGTGA AAGTGAAAAA GGACAAGCAC AATTTTATAA TTCATTTAAA AGATCCTAAG 
AAAATTGTGA GAAACGAAAA AAAAGAAGAC GATTATTCTT CAGAAGATGA GAAAGATGAA 
GGAGAGGAAG AACGAAATCT TGAAATGAAA GAATCAGATG CAGAGAATAA AGCTATCAGC 
AAGTTTGGAT CTTTTTCTGC CACCTTGGAA AATGGAATCT GCCTCTCAAT AAGTTACTAC 
GGATCAAGTG GAATGGCGCC AGAGGATAAG GATCCTAATT TGGAAGCAAT GTTGAATATC 
CCGTCAGCAC TCACTCCAAC AATAATTCCT GTGGTAGTGA CTGTTCCTCA AGGCAAAGGA 
AAAGGGAAAA CCAAAGGCAA AGAAAAGCCC AAAGACTCTA TTAAAGAAGA GGAACATCCA 
AAAGAAGAAG AGAAAAAGGA AGAAGTAGAA CCAGAACCTG TTTTACAAGA GACTGTTTAT 
GTTCCCACCT TCCAAAACCT AAATGTGTCT TGCCCCAGTG GGCTTCTAGT GACCTTCATT 
GGCCAAGAGT CTGCAGATCA CCATGTTATG GATGAGGAAC CCACTTGGGA CATCATGGTC 
CGACAGAGCT ACCCCCAGAA GGTGAAGCAC TATGAGTTCT ACAAAACGGT GATGCCACCA 
GTGGAGCAGG AGGCATCGAG AGTTATCACT AGTCAAGGCA CTGTTGTCAA ATACATGTTG 
GATGGATCCA CACAGATTCT CTTTGCAAAT GGCGCTGTGA GCAGTAGTCC CGATTCAGGT 
CCTGTTTGTC CTCCTCCTGA AATGCCAGCG AGCCCTCATA ACGGAGACTT GATGGACAGT 
GTTTCTCAGC AAAAATCAGA AACAGCACCG TCTGAGACTG TCATCACAAA GAAAGGAAAA 
GGTCACAAAA ATCAGTCATC AGTGCCACAT ACGAATGAAA TCCATGAACC TCCCCAAGAG 
GTAGTTCAAA TAGTAACACC TGTGGAGGTT CATGTAGGCA CCTGGTTTAC AACTACACCT 
GACGGAAATC GGATTGGCAC CAAAGGATTA GAAAAAATAG CAGACTTGGC CCCATTCTTA 
TCCTTTCAGG CCACAGATCC TGTCAATGGA ACGGTTATGA CAACTCGAGA GGACAAGGTG 
ATCATTGTTG AAAAGAAGGA TGGTACTCGG ATCGTGGATC ATGCCGATGG TACCAGAATC 
ACAACCTTTT ATCAAGTTTA TGAAGATCAT ATTATGCCTC CAGATGATCA AGAAATACCC 
GAACATCCTC AAACTCTCAC CAGGCAGGTG AAGTGCATGC GCATAGAAAG CTCACACTAT 
GCCACTGTCA TCACCAACTG CGAGGACAGT AGCTGCTGTG CCACTTTTGG AGATGGGACG 
TCTATTATTG CAAAGCCACA GGGAACATAC CAGGTGTTAC CTTCAAACAA AGGCTGTCTT 
CATATTGATA GAGACTGTTC AGCTGTATAT TACCATGAAT CCAGCAGTGA TATATACCAT 
CCTTTCCAAA AACGTGAGCA GCTGAGAGCT AGCAGGTACA TCATGAAGCA CACGTCAGAG 
GTTCTCTGTG AAGTCCTGGA TCCTGAGGGA AACACCTTTC AGGTCATGGC TGATGGTAGC 
ACATCAACTC TGCTACCTGA AAACAATTTG GAAGATGATT TAAACTCAAA ACCTGAAGGC 
TATGATAGTT TATCATCTAC TCACCTTCAT AAAAATCACC TACAAATATA TGGTGAACAT 
GTCCCCAGGT TTTTTGTTAT CTATGCCGAT GGATCAGGAG TAGAACTTCT TCGAGATGAT 
GACATAGAAG AATACTTATC CTTGGCATAT GGAGAATCAA CCACTGTAGT TCTTCAAGAG 
CCAGTACAAG AACAGCCAGG CGCCCTGAGC ATCACTGTTC TTCGCCCTTT TCATGAAGCA 
TCGCCATGGC TAATGAAGAA AGAATTAGAC ACAATTGTTC CTCCTAATCT CCAGTCAAGG 
TCATGGGAGA AATTTCCCTC AGTTGAGAAA AAAACTCCAG GACCTCCCTT TGGCACTCAT 
ATTTGGAAGG GCCTTAACAT TGAGTGCAAA CAGCTAGTGA GTGCCCCAGC TCCTATACTC 
AAAAGCCCCA ATGTACTACA GATACGCCAG TTCATCCAGC ATGAGGTCAT AAACGATGAG 
GTGAAACTGA AGCTGCAGAT TTCCCTTAAG GACTATATAA ACCATATTTT AAAGAAAGAA 
GATGAGCTAC AGGAAATGAC AGTTAAAGAT TCCAGAACTG AGGAGGAGAG AGGCAATGCT 
GCAGATCTCC TCAAGCTTGT TATGTCTTTT CCTAAAATGG AGGAAACTAC AAAAGGTCAT 
GCTACTGAAG TTGCAGCCCA CTTAGTTGAT TTATTCAAGC AATCTTTGGC TGTTGCTCCA 
GAATGTCCAC CAGAAACATT TGATACAGAT TTCTTGGAAA AGAAAGGGAG GAAGGAAATC 
GAAAAAACAC ACAATTATCT AGAGGAAATC AGGAACAGAA CGGTATCACC CTACTTCAAA 
TCAGAATTGA GCAAGACCTT TCAGTCTCAG TTTAATTATC TAGACGCCCT TTCCAAAAAC 
TTGCCTCCTT TTACAAAGAA AGTTGAAGAT ACAAACAAAA CAGCTGTTCA AAATACATCC 
AATCTTCAGC CAGATTCCCA GCCAAATAAA GTTTCACAAC AAGAATCCTC AGACACACTT 
AGTCTTCCAA AATCAGAGGC TTTTGTGGAT ATGAAGGCGT TCGCTAGCCA GAACCAAACT 
GAAAATTTAA CAAAATCTAC TGAAGAACCA GAAACTTATG CCCCTGTGAA AATTCCAACC 
CAGTCATTGC TGCAGGATGT TGCAGGACAA GCAAGGAAAG AGAAAGTGAG GTTACCTCGC 
TATTTGCTGA GTTCCAAACC CAAGTCTCAA CCTCTCGAGA AGGTGCAAGA TTCTGTTGGA 
GGAAGAGTGA GAACATCCTC GGTTGCTTCT GCTGCCATTA ATAACGCAAA TTCATCCCCT 
TTTGGATTTC ATCTTCTCCC ACCGTCAGTG AATTTTGGAG TGCTTGAAGA AGGGCACACC 
TATGAAACCA CTGTGAAGCT CAAGAATGTT GGAGTGGACT TCTGCAGGTT TAGGGTGAAG 
CAGCCGCCAC CCAGCACAGG ATTGAAAGTG ACTTACAAAC CTGGGCCTGT GGCAGCTGGT 
TTGCAGGCAG AACTGAAAGT GGAGTTATTT ACCATGGTTG TTGGAGAGGA TGGTGCCAAG 
GGATCAGCAC ACATCTCTCA CAATATTGAG ATTATGACTG AGCATGATGT CCTGTTCTTA 
CCTGTGGAAG CAACAGTTTT AACAAGCAGC AATTATGATA AGCGATTAAA AGATTTTCCC 
CAGGAAAAAG AAAACCCCAC GATCCAGAGG ACTTCTACAA TTTCTTCTGC TAAACTTGGA 
GTCTTCATGT CTCGTAAAGT TTCTCCTTAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023497097.1
CDS	67..6699
Translation	MAPKKEKSGSASANSKIWEPSLIAAHFNQNDWKGVIAFVVGNRIEDELLIGALTLAVQAPQRRLFSMVSWQDILQQINGTNALPGTASKKGKKPLGGSAPLYSEVLIATKAIMDSGEKLTLPLIGKLLKYQLLQIKYKDQQRRESEKKVTEDKFKTEKDKGKAKSPKEKKPSGTKVVKGKGKDQPEANTTVKKATQLKQRGEEDDAKRYIDDEPDDGAQHYIIVVGFNDPQLLAIMTELGIPISSVIKISSENYEPLQAHLAAVSQQQEVSLQPEDIEAEKLKNENTIKELEIFWKYLEPILNNEKPETNLFDVARLEYMVKEDGFPSDWSDSGMMLELGTDIFENIACLMYDSLDWKRQHRHYLESMQLINVPQVVNEKPILEAVAIAEAPQAAAPTPGKKKAQNEERQAPTSVPFVITTEADMRYYNDLLNPIPEEFISVPLILHCMLEQVVATEEDLVPPSLVEPPPRADGLDHRIAAHIVSILPSLCLTEKEKKNLQEIFLSEEENESKAVPKGPLLLNYHDTLAHKKYALKDPKNFDPVQIEQEMQSKLPLWEFLEFPLPPPWNSTKRLATIHELMHFCTNEVLSWNEVERAFKVFTFESLKLSTVDEKGKLKPARMMYGTDCEMFNIPWDNPARFAKQIRQQYITKMNTQEVKQKTDTETKDRILFVDQNMPVSVQDDESSKEPSDPSQADASNMQHSDSNNKKSLDPDDRELSEQDNSLKTQLQSEPLEQTTGNEIKDEAVTNVDPPEKKSKKMTVEAELEDIKKTEQRSLMDWSFTEHFKPKVLLQVLQEANQHYRCIDSYYHTQDNSLLLVFHNPMNLQRLHCEYWNVALHSNVGFRNYLELVAQSIQDWIIKEEAIYQESKMAEELSRSKLEQEVKSPASGKISSPSKIYSVKKSKSNKGMSKTELIDQEKEKEKEKEKIPFILEGSLKAWKEEQDRLAEEERLKEEKKAEKKSKDIGKKKGKEKVEREDSRVLKKKSFFKDKAKEEQIKISDVTEEPLQQPEPEIVYPFRGYNMGDIPIQISGTNCYLYPSDGGQIEVEKTTFEKGPTFIKVKVKKDKHNFIIHLKDPKKIVRNEKKEDDYSSEDEKDEGEEERNLEMKESDAENKAISKFGSFSATLENGICLSISYYGSSGMAPEDKDPNLEAMLNIPSALTPTIIPVVVTVPQGKGKGKTKGKEKPKDSIKEEEHPKEEEKKEEVEPEPVLQETVYVPTFQNLNVSCPSGLLVTFIGQESADHHVMDEEPTWDIMVRQSYPQKVKHYEFYKTVMPPVEQEASRVITSQGTVVKYMLDGSTQILFANGAVSSSPDSGPVCPPPEMPASPHNGDLMDSVSQQKSETAPSETVITKKGKGHKNQSSVPHTNEIHEPPQEVVQIVTPVEVHVGTWFTTTPDGNRIGTKGLEKIADLAPFLSFQATDPVNGTVMTTREDKVIIVEKKDGTRIVDHADGTRITTFYQVYEDHIMPPDDQEIPEHPQTLTRQVKCMRIESSHYATVITNCEDSSCCATFGDGTSIIAKPQGTYQVLPSNKGCLHIDRDCSAVYYHESSSDIYHPFQKREQLRASRYIMKHTSEVLCEVLDPEGNTFQVMADGSTSTLLPENNLEDDLNSKPEGYDSLSSTHLHKNHLQIYGEHVPRFFVIYADGSGVELLRDDDIEEYLSLAYGESTTVVLQEPVQEQPGALSITVLRPFHEASPWLMKKELDTIVPPNLQSRSWEKFPSVEKKTPGPPFGTHIWKGLNIECKQLVSAPAPILKSPNVLQIRQFIQHEVINDEVKLKLQISLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKGHATEVAAHLVDLFKQSLAVAPECPPETFDTDFLEKKGRKEIEKTHNYLEEIRNRTVSPYFKSELSKTFQSQFNYLDALSKNLPPFTKKVEDTNKTAVQNTSNLQPDSQPNKVSQQESSDTLSLPKSEAFVDMKAFASQNQTENLTKSTEEPETYAPVKIPTQSLLQDVAGQARKEKVRLPRYLLSSKPKSQPLEKVQDSVGGRVRTSSVASAAINNANSSPFGFHLLPPSVNFGVLEEGHTYETTVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQAELKVELFTMVVGEDGAKGSAHISHNIEIMTEHDVLFLPVEATVLTSSNYDKRLKDFPQEKENPTIQRTSTISSAKLGVFMSRKVSPY

Target ORF information:

RefSeq Version	XM_023641329.1
Organism	Equus caballus(horse)
Definition	Equus caballus sperm associated antigen 17 (SPAG17), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023641329.1

ORF Insert Sequence:

ATGGCACCCA AGAAAGAGAA AAGCGGGTCG GCGAGCGCGA ATTCTAAGAT ATGGGAACCC 
TCGCTCATCG CTGCACACTT CAATCAGAAC GATTGGAAGG GCGTCATCGC TTTTGTAGTT 
GGAAACCGGA TTGAGGATGA GCTTCTCATT GGAGCCCTCA CCCTCGCTGT CCAGGCCCCT 
CAGCGTAGAC TCTTCAGTAT GGTATCATGG CAAGACATCC TCCAGCAGAT CAATGGAACA 
AATGCGCTTC CTGGAACTGC ATCTAAAAAG GGAAAGAAAC CTCTAGGCGG TAGCGCTCCC 
TTATATTCTG AGGTGTTAAT AGCAACAAAG GCAATTATGG ACAGTGGAGA GAAATTAACC 
TTACCGCTGA TAGGGAAACT CTTGAAATAT CAGCTTCTCC AGATTAAATA TAAGGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGGTGACA GAAGACAAAT TTAAGACTGA AAAGGATAAA 
GGGAAAGCGA AATCTCCCAA GGAAAAGAAG CCCTCAGGTA CCAAGGTTGT CAAAGGAAAG 
GGAAAGGATC AGCCTGAGGC AAACACAACA GTGAAAAAGG CCACTCAGTT AAAACAGAGG 
GGAGAAGAAG ACGACGCCAA ACGTTACATT GATGATGAGC CAGATGATGG TGCCCAACAT 
TACATTATAG TTGTGGGCTT CAACGATCCT CAGCTATTAG CGATTATGAC TGAGCTTGGC 
ATTCCCATAA GCAGCGTGAT TAAAATATCT TCAGAGAATT ATGAACCTCT GCAGGCGCAC 
CTGGCAGCAG TTAGCCAGCA GCAGGAAGTT TCTCTTCAGC CAGAAGATAT AGAAGCTGAA 
AAATTGAAGA ATGAGAATAC CATAAAAGAA CTTGAAATTT TCTGGAAGTA CTTGGAACCA 
ATCCTGAATA ATGAGAAACC TGAAACAAAT CTCTTTGATG TTGCTCGTCT TGAATACATG 
GTCAAGGAAG ATGGCTTTCC TTCTGACTGG TCAGACAGTG GGATGATGCT GGAATTGGGC 
ACCGACATTT TTGAAAATAT TGCCTGTTTG ATGTACGACA GCCTGGATTG GAAAAGGCAG 
CACCGGCACT ATTTAGAAAG CATGCAGCTT ATTAATGTCC CACAAGTGGT TAACGAGAAG 
CCTATCTTAG AAGCTGTAGC CATTGCTGAG GCTCCACAAG CTGCTGCTCC AACCCCTGGG 
AAGAAGAAAG CACAAAATGA AGAACGGCAA GCCCCAACAT CAGTGCCTTT TGTCATCACA 
ACTGAAGCAG ACATGAGATA TTACAATGAT TTGCTGAATC CGATTCCAGA AGAATTCATT 
TCTGTGCCCC TGATACTGCA CTGTATGCTG GAACAGGTTG TAGCAACTGA AGAAGACCTT 
GTCCCACCCA GTCTGGTGGA GCCGCCTCCC AGAGCAGATG GGTTGGACCA CAGAATTGCA 
GCTCACATTG TATCCATCCT ACCCTCTCTC TGTCTCACGG AGAAGGAGAA AAAGAATCTC 
CAGGAAATAT TTTTGTCTGA AGAAGAAAAT GAAAGCAAAG CAGTGCCCAA AGGTCCTCTC 
CTACTGAACT ATCACGACAC TCTGGCCCAC AAGAAGTATG CACTGAAGGA CCCAAAGAAT 
TTCGACCCAG TTCAAATTGA GCAGGAGATG CAGTCCAAGT TGCCACTGTG GGAATTCCTT 
GAATTCCCTC TGCCCCCGCC ATGGAACAGC ACTAAACGTC TAGCTACAAT TCATGAGCTC 
ATGCACTTTT GTACAAATGA AGTCTTGAGC TGGAATGAAG TAGAACGAGC CTTCAAAGTG 
TTTACTTTTG AGAGCCTGAA ACTCTCTACA GTTGATGAAA AAGGGAAACT AAAGCCTGCT 
CGGATGATGT ATGGGACTGA TTGTGAGATG TTCAACATAC CATGGGACAA CCCTGCCAGA 
TTTGCTAAAC AGATAAGGCA ACAATACATC ACGAAAATGA ACACCCAAGA GGTCAAACAG 
AAAACAGATA CTGAAACCAA AGACAGAATT TTGTTTGTGG ATCAGAATAT GCCAGTGTCT 
GTGCAAGATG ATGAGAGCAG TAAAGAACCT TCAGACCCTA GTCAGGCTGA TGCTAGCAAT 
ATGCAGCATT CTGACTCGAA TAATAAGAAA TCCTTAGACC CTGATGATAG AGAGCTGTCA 
GAGCAGGACA ACAGCTTGAA GACTCAGCTC CAATCTGAGC CTTTGGAGCA GACCACGGGC 
AATGAGATCA AAGATGAAGC AGTCACAAAT GTTGATCCTC CTGAAAAGAA ATCCAAGAAG 
ATGACCGTGG AAGCAGAGTT GGAGGACATA AAGAAAACAG AGCAACGCAG CCTAATGGAT 
TGGAGTTTCA CTGAACATTT TAAACCGAAA GTTCTACTGC AGGTTCTTCA AGAAGCCAAT 
CAGCATTACA GATGTATTGA TTCTTATTAC CACACCCAAG ACAACTCTTT ACTATTAGTC 
TTTCATAATC CAATGAATTT ACAACGTCTG CATTGTGAAT ATTGGAATGT TGCTCTTCAC 
TCCAATGTTG GATTCAGGAA TTATTTGGAA CTTGTTGCAC AGTCCATTCA AGACTGGATC 
ATAAAAGAAG AAGCTATATA TCAGGAATCT AAAATGGCTG AGGAACTCAG CAGGAGCAAG 
CTTGAGCAGG AAGTGAAATC TCCTGCTAGT GGCAAAATTT CTTCCCCTAG CAAAATTTAT 
TCTGTTAAAA AATCTAAAAG TAACAAAGGA ATGAGCAAAA CAGAGTTAAT AGATCAAGAA 
AAAGAAAAAG AGAAAGAGAA GGAAAAGATT CCTTTCATTT TAGAAGGCTC TCTCAAGGCA 
TGGAAAGAAG AGCAAGATCG ATTAGCAGAA GAGGAACGCT TAAAGGAAGA AAAAAAAGCA 
GAGAAGAAGA GTAAGGACAT TGGTAAAAAG AAAGGCAAGG AAAAGGTAGA GAGAGAAGAT 
AGCAGGGTTT TGAAGAAAAA ATCTTTTTTC AAGGATAAAG CTAAAGAAGA GCAAATCAAG 
ATCTCAGACG TAACAGAGGA GCCCCTTCAA CAGCCAGAAC CTGAGATAGT TTACCCGTTT 
CGCGGATACA ATATGGGAGA TATACCCATC CAAATCTCGG GAACAAATTG TTATCTGTAT 
CCTTCAGATG GAGGGCAGAT TGAAGTAGAA AAGACAACAT TTGAAAAAGG TCCAACTTTT 
ATCAAAGTGA AAGTGAAAAA GGACAAGCAC AATTTTATAA TTCATTTAAA AGATCCTAAG 
AAAATTGTGA GAAACGAAAA AAAAGAAGAC GATTATTCTT CAGAAGATGA GAAAGATGAA 
GGAGAGGAAG AACGAAATCT TGAAATGAAA GAATCAGATG CAGAGAATAA AGCTATCAGC 
AAGTTTGGAT CTTTTTCTGC CACCTTGGAA AATGGAATCT GCCTCTCAAT AAGTTACTAC 
GGATCAAGTG GAATGGCGCC AGAGGATAAG GATCCTAATT TGGAAGCAAT GTTGAATATC 
CCGTCAGCAC TCACTCCAAC AATAATTCCT GTGGTAGTGA CTGTTCCTCA AGGCAAAGGA 
AAAGGGAAAA CCAAAGGCAA AGAAAAGCCC AAAGACTCTA TTAAAGAAGA GGAACATCCA 
AAAGAAGAAG AGAAAAAGGA AGAAGTAGAA CCAGAACCTG TTTTACAAGA GACTGTTTAT 
GTTCCCACCT TCCAAAACCT AAATGTGTCT TGCCCCAGTG GGCTTCTAGT GACCTTCATT 
GGCCAAGAGT CTGCAGATCA CCATGTTATG GATGAGGAAC CCACTTGGGA CATCATGGTC 
CGACAGAGCT ACCCCCAGAA GGTGAAGCAC TATGAGTTCT ACAAAACGGT GATGCCACCA 
GTGGAGCAGG AGGCATCGAG AGTTATCACT AGTCAAGGCA CTGTTGTCAA ATACATGTTG 
GATGGATCCA CACAGATTCT CTTTGCAAAT GGCGCTGTGA GCAGTAGTCC CGATTCAGGT 
CCTGTTTGTC CTCCTCCTGA AATGCCAGCG AGCCCTCATA ACGGAGACTT GATGGACAGT 
GTTTCTCAGC AAAAATCAGA AACAGCACCG TCTGAGACTG TCATCACAAA GAAAGGAAAA 
GGTCACAAAA ATCAGTCATC AGTGCCACAT ACGAATGAAA TCCATGAACC TCCCCAAGAG 
GTAGTTCAAA TAGTAACACC TGTGGAGGTT CATGTAGGCA CCTGGTTTAC AACTACACCT 
GACGGAAATC GGATTGGCAC CAAAGGATTA GAAAAAATAG CAGACTTGGC CCCATTCTTA 
TCCTTTCAGG CCACAGATCC TGTCAATGGA ACGGTTATGA CAACTCGAGA GGACAAGGTG 
ATCATTGTTG AAAAGAAGGA TGGTACTCGG ATCGTGGATC ATGCCGATGG TACCAGAATC 
ACAACCTTTT ATCAAGTTTA TGAAGATCAT ATTATGCCTC CAGATGATCA AGAAATACCC 
GAACATCCTC AAACTCTCAC CAGGCAGGTG AAGTGCATGC GCATAGAAAG CTCACACTAT 
GCCACTGTCA TCACCAACTG CGAGGACAGT AGCTGCTGTG CCACTTTTGG AGATGGGACG 
TCTATTATTG CAAAGCCACA GGGAACATAC CAGGTGTTAC CTTCAAACAA AGGCTGTCTT 
CATATTGATA GAGACTGTTC AGCTGTATAT TACCATGAAT CCAGCAGTGA TATATACCAT 
CCTTTCCAAA AACGTGAGCA GCTGAGAGCT AGCAGGTACA TCATGAAGCA CACGTCAGAG 
GTTCTCTGTG AAGTCCTGGA TCCTGAGGGA AACACCTTTC AGGTCATGGC TGATGGTAGC 
ACATCAACTC TGCTACCTGA AAACAATTTG GAAGATGATT TAAACTCAAA ACCTGAAGGC 
TATGATAGTT TATCATCTAC TCACCTTCAT AAAAATCACC TACAAATATA TGGTGAACAT 
GTCCCCAGGT TTTTTGTTAT CTATGCCGAT GGATCAGGAG TAGAACTTCT TCGAGATGAT 
GACATAGAAG AATACTTATC CTTGGCATAT GGAGAATCAA CCACTGTAGT TCTTCAAGAG 
CCAGTACAAG AACAGCCAGG CGCCCTGAGC ATCACTGTTC TTCGCCCTTT TCATGAAGCA 
TCGCCATGGC TAATGAAGAA AGAATTAGAC ACAATTGTTC CTCCTAATCT CCAGTCAAGG 
TCATGGGAGA AATTTCCCTC AGTTGAGAAA AAAACTCCAG GACCTCCCTT TGGCACTCAT 
ATTTGGAAGG GCCTTAACAT TGAGTGCAAA CAGCTAGTGA GTGCCCCAGC TCCTATACTC 
AAAAGCCCCA ATGTACTACA GATACGCCAG TTCATCCAGC ATGAGGTCAT AAACGATGAG 
GTGAAACTGA AGCTGCAGAT TTCCCTTAAG GACTATATAA ACCATATTTT AAAGAAAGAA 
GATGAGCTAC AGGAAATGAC AGTTAAAGAT TCCAGAACTG AGGAGGAGAG AGGCAATGCT 
GCAGATCTCC TCAAGCTTGT TATGTCTTTT CCTAAAATGG AGGAAACTAC AAAAGGTCAT 
GCTACTGAAG TTGCAGCCCA CTTAGTTGAT TTATTCAAGC AATCTTTGGC TGTTGCTCCA 
GAATGTCCAC CAGAAACATT TGATACAGAT TTCTTGGAAA AGAAAGGGAG GAAGGAAATC 
GAAAAAACAC ACAATTATCT AGAGGAAATC AGGAACAGAA CGGTATCACC CTACTTCAAA 
TCAGAATTGA GCAAGACCTT TCAGTCTCAG TTTAATTATC TAGACGCCCT TTCCAAAAAC 
TTGCCTCCTT TTACAAAGAA AGTTGAAGAT ACAAACAAAA CAGCTGTTCA AAATACATCC 
AATCTTCAGC CAGATTCCCA GCCAAATAAA GTTTCACAAC AAGAATCCTC AGACACACTT 
AGTCTTCCAA AATCAGAGGC TTTTGTGGAT ATGAAGGCGT TCGCTAGCCA GAACCAAACT 
GAAAATTTAA CAAAATCTAC TGAAGAACCA GAAACTTATG CCCCTGTGAA AATTCCAACC 
CAGTCATTGC TGCAGGATGT TGCAGGACAA GCAAGGAAAG AGAAAGTGAG GTTACCTCGC 
TATTTGCTGA GTTCCAAACC CAAGTCTCAA CCTCTCGAGA AGGTGCAAGA TTCTGTTGGA 
GGAAGAGTGA GAACATCCTC GGTTGCTTCT GCTGCCATTA ATAACGCAAA TTCATCCCCT 
TTTGGATTTC ATCTTCTCCC ACCGTCAGTG AATTTTGGAG TGCTTGAAGA AGGGCACACC 
TATGAAACCA CTGTGAAGCT CAAGAATGTT GGAGTGGACT TCTGCAGGTT TAGGGTGAAG 
CAGCCGCCAC CCAGCACAGG ATTGAAAGTG ACTTACAAAC CTGGGCCTGT GGCAGCTGGT 
TTGCAGGCAG AACTGAAAGT GGAGTTATTT ACCATGGTTG TTGGAGAGGA TGGTGCCAAG 
GGATCAGCAC ACATCTCTCA CAATATTGAG ATTATGACTG AGCATGATGT CCTGTTCTTA 
CCTGTGGAAG CAACAGTTTT AACAAGCAGC AATTATGATA AGCGATTAAA AGATTTTCCC 
CAGGAAAAAG AAAACCCCAC GATCCAGAGG ACTTCTACAA TTTCTTCTGC TAAACTTGGA 
GTCTTCATGT CTCGTAAAGT TTCTCCTTAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_023641330.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023497098.1 Equus caballus sperm associated antigen 17 (SPAG17), transcript variant X3, mRNA.

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CloneID	OHb65450
Clone ID Related Accession (Same CDS sequence)	XM_023641330.1
Accession Version	XM_023641330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	sperm-associated antigen 17 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCA AGAAAGAGAA AAGCGGGTCG GCGAGCGCGA ATTCTAAGAT ATGGGAACCC 
TCGCTCATCG CTGCACACTT CAATCAGAAC GATTGGAAGG GCGTCATCGC TTTTGTAGTT 
GGAAACCGGA TTGAGGATGA GCTTCTCATT GGAGCCCTCA CCCTCGCTGT CCAGGCCCCT 
CAGCGTAGAC TCTTCAGTAT GGTATCATGG CAAGACATCC TCCAGCAGAT CAATGGAACA 
AATGCGCTTC CTGGAACTGC ATCTAAAAAG GGAAAGAAAC CTCTAGGCGG TAGCGCTCCC 
TTATATTCTG AGGTGACAGA AGACAAATTT AAGACTGAAA AGGATAAAGG GAAAGCGAAA 
TCTCCCAAGG AAAAGAAGCC CTCAGGTACC AAGGTTGTCA AAGGAAAGGG AAAGGATCAG 
CCTGAGGCAA ACACAACAGT GAAAAAGGCC ACTCAGTTAA AACAGAGGGG AGAAGAAGAC 
GACGCCAAAC GTTACATTGA TGATGAGCCA GATGATGGTG CCCAACATTA CATTATAGTT 
GTGGGCTTCA ACGATCCTCA GCTATTAGCG ATTATGACTG AGCTTGGCAT TCCCATAAGC 
AGCGTGATTA AAATATCTTC AGAGAATTAT GAACCTCTGC AGGCGCACCT GGCAGCAGTT 
AGCCAGCAGC AGGAAGTTTC TCTTCAGCCA GAAGATATAG AAGCTGAAAA ATTGAAGAAT 
GAGAATACCA TAAAAGAACT TGAAATTTTC TGGAAGTACT TGGAACCAAT CCTGAATAAT 
GAGAAACCTG AAACAAATCT CTTTGATGTT GCTCGTCTTG AATACATGGT CAAGGAAGAT 
GGCTTTCCTT CTGACTGGTC AGACAGTGGG ATGATGCTGG AATTGGGCAC CGACATTTTT 
GAAAATATTG CCTGTTTGAT GTACGACAGC CTGGATTGGA AAAGGCAGCA CCGGCACTAT 
TTAGAAAGCA TGCAGCTTAT TAATGTCCCA CAAGTGGTTA ACGAGAAGCC TATCTTAGAA 
GCTGTAGCCA TTGCTGAGGC TCCACAAGCT GCTGCTCCAA CCCCTGGGAA GAAGAAAGCA 
CAAAATGAAG AACGGCAAGC CCCAACATCA GTGCCTTTTG TCATCACAAC TGAAGCAGAC 
ATGAGATATT ACAATGATTT GCTGAATCCG ATTCCAGAAG AATTCATTTC TGTGCCCCTG 
ATACTGCACT GTATGCTGGA ACAGGTTGTA GCAACTGAAG AAGACCTTGT CCCACCCAGT 
CTGGTGGAGC CGCCTCCCAG AGCAGATGGG TTGGACCACA GAATTGCAGC TCACATTGTA 
TCCATCCTAC CCTCTCTCTG TCTCACGGAG AAGGAGAAAA AGAATCTCCA GGAAATATTT 
TTGTCTGAAG AAGAAAATGA AAGCAAAGCA GTGCCCAAAG GTCCTCTCCT ACTGAACTAT 
CACGACACTC TGGCCCACAA GAAGTATGCA CTGAAGGACC CAAAGAATTT CGACCCAGTT 
CAAATTGAGC AGGAGATGCA GTCCAAGTTG CCACTGTGGG AATTCCTTGA ATTCCCTCTG 
CCCCCGCCAT GGAACAGCAC TAAACGTCTA GCTACAATTC ATGAGCTCAT GCACTTTTGT 
ACAAATGAAG TCTTGAGCTG GAATGAAGTA GAACGAGCCT TCAAAGTGTT TACTTTTGAG 
AGCCTGAAAC TCTCTACAGT TGATGAAAAA GGGAAACTAA AGCCTGCTCG GATGATGTAT 
GGGACTGATT GTGAGATGTT CAACATACCA TGGGACAACC CTGCCAGATT TGCTAAACAG 
ATAAGGCAAC AATACATCAC GAAAATGAAC ACCCAAGAGG TCAAACAGAA AACAGATACT 
GAAACCAAAG ACAGAATTTT GTTTGTGGAT CAGAATATGC CAGTGTCTGT GCAAGATGAT 
GAGAGCAGTA AAGAACCTTC AGACCCTAGT CAGGCTGATG CTAGCAATAT GCAGCATTCT 
GACTCGAATA ATAAGAAATC CTTAGACCCT GATGATAGAG AGCTGTCAGA GCAGGACAAC 
AGCTTGAAGA CTCAGCTCCA ATCTGAGCCT TTGGAGCAGA CCACGGGCAA TGAGATCAAA 
GATGAAGCAG TCACAAATGT TGATCCTCCT GAAAAGAAAT CCAAGAAGAT GACCGTGGAA 
GCAGAGTTGG AGGACATAAA GAAAACAGAG CAACGCAGCC TAATGGATTG GAGTTTCACT 
GAACATTTTA AACCGAAAGT TCTACTGCAG GTTCTTCAAG AAGCCAATCA GCATTACAGA 
TGTATTGATT CTTATTACCA CACCCAAGAC AACTCTTTAC TATTAGTCTT TCATAATCCA 
ATGAATTTAC AACGTCTGCA TTGTGAATAT TGGAATGTTG CTCTTCACTC CAATGTTGGA 
TTCAGGAATT ATTTGGAACT TGTTGCACAG TCCATTCAAG ACTGGATCAT AAAAGAAGAA 
GCTATATATC AGGAATCTAA AATGGCTGAG GAACTCAGCA GGAGCAAGCT TGAGCAGGAA 
GTGAAATCTC CTGCTAGTGG CAAAATTTCT TCCCCTAGCA AAATTTATTC TGTTAAAAAA 
TCTAAAAGTA ACAAAGGAAT GAGCAAAACA GAGTTAATAG ATCAAGAAAA AGAAAAAGAG 
AAAGAGAAGG AAAAGATTCC TTTCATTTTA GAAGGCTCTC TCAAGGCATG GAAAGAAGAG 
CAAGATCGAT TAGCAGAAGA GGAACGCTTA AAGGAAGAAA AAAAAGCAGA GAAGAAGAGT 
AAGGACATTG GTAAAAAGAA AGGCAAGGAA AAGGTAGAGA GAGAAGATAG CAGGGTTTTG 
AAGAAAAAAT CTTTTTTCAA GGATAAAGCT AAAGAAGAGC AAATCAAGAT CTCAGACGTA 
ACAGAGGAGC CCCTTCAACA GCCAGAACCT GAGATAGTTT ACCCGTTTCG CGGATACAAT 
ATGGGAGATA TACCCATCCA AATCTCGGGA ACAAATTGTT ATCTGTATCC TTCAGATGGA 
GGGCAGATTG AAGTAGAAAA GACAACATTT GAAAAAGGTC CAACTTTTAT CAAAGTGAAA 
GTGAAAAAGG ACAAGCACAA TTTTATAATT CATTTAAAAG ATCCTAAGAA AATTGTGAGA 
AACGAAAAAA AAGAAGACGA TTATTCTTCA GAAGATGAGA AAGATGAAGG AGAGGAAGAA 
CGAAATCTTG AAATGAAAGA ATCAGATGCA GAGAATAAAG CTATCAGCAA GTTTGGATCT 
TTTTCTGCCA CCTTGGAAAA TGGAATCTGC CTCTCAATAA GTTACTACGG ATCAAGTGGA 
ATGGCGCCAG AGGATAAGGA TCCTAATTTG GAAGCAATGT TGAATATCCC GTCAGCACTC 
ACTCCAACAA TAATTCCTGT GGTAGTGACT GTTCCTCAAG GCAAAGGAAA AGGGAAAACC 
AAAGGCAAAG AAAAGCCCAA AGACTCTATT AAAGAAGAGG AACATCCAAA AGAAGAAGAG 
AAAAAGGAAG AAGTAGAACC AGAACCTGTT TTACAAGAGA CTGTTTATGT TCCCACCTTC 
CAAAACCTAA ATGTGTCTTG CCCCAGTGGG CTTCTAGTGA CCTTCATTGG CCAAGAGTCT 
GCAGATCACC ATGTTATGGA TGAGGAACCC ACTTGGGACA TCATGGTCCG ACAGAGCTAC 
CCCCAGAAGG TGAAGCACTA TGAGTTCTAC AAAACGGTGA TGCCACCAGT GGAGCAGGAG 
GCATCGAGAG TTATCACTAG TCAAGGCACT GTTGTCAAAT ACATGTTGGA TGGATCCACA 
CAGATTCTCT TTGCAAATGG CGCTGTGAGC AGTAGTCCCG ATTCAGGTCC TGTTTGTCCT 
CCTCCTGAAA TGCCAGCGAG CCCTCATAAC GGAGACTTGA TGGACAGTGT TTCTCAGCAA 
AAATCAGAAA CAGCACCGTC TGAGACTGTC ATCACAAAGA AAGGAAAAGG TCACAAAAAT 
CAGTCATCAG TGCCACATAC GAATGAAATC CATGAACCTC CCCAAGAGGT AGTTCAAATA 
GTAACACCTG TGGAGGTTCA TGTAGGCACC TGGTTTACAA CTACACCTGA CGGAAATCGG 
ATTGGCACCA AAGGATTAGA AAAAATAGCA GACTTGGCCC CATTCTTATC CTTTCAGGCC 
ACAGATCCTG TCAATGGAAC GGTTATGACA ACTCGAGAGG ACAAGGTGAT CATTGTTGAA 
AAGAAGGATG GTACTCGGAT CGTGGATCAT GCCGATGGTA CCAGAATCAC AACCTTTTAT 
CAAGTTTATG AAGATCATAT TATGCCTCCA GATGATCAAG AAATACCCGA ACATCCTCAA 
ACTCTCACCA GGCAGGTGAA GTGCATGCGC ATAGAAAGCT CACACTATGC CACTGTCATC 
ACCAACTGCG AGGACAGTAG CTGCTGTGCC ACTTTTGGAG ATGGGACGTC TATTATTGCA 
AAGCCACAGG GAACATACCA GGTGTTACCT TCAAACAAAG GCTGTCTTCA TATTGATAGA 
GACTGTTCAG CTGTATATTA CCATGAATCC AGCAGTGATA TATACCATCC TTTCCAAAAA 
CGTGAGCAGC TGAGAGCTAG CAGGTACATC ATGAAGCACA CGTCAGAGGT TCTCTGTGAA 
GTCCTGGATC CTGAGGGAAA CACCTTTCAG GTCATGGCTG ATGGTAGCAC ATCAACTCTG 
CTACCTGAAA ACAATTTGGA AGATGATTTA AACTCAAAAC CTGAAGGCTA TGATAGTTTA 
TCATCTACTC ACCTTCATAA AAATCACCTA CAAATATATG GTGAACATGT CCCCAGGTTT 
TTTGTTATCT ATGCCGATGG ATCAGGAGTA GAACTTCTTC GAGATGATGA CATAGAAGAA 
TACTTATCCT TGGCATATGG AGAATCAACC ACTGTAGTTC TTCAAGAGCC AGTACAAGAA 
CAGCCAGGCG CCCTGAGCAT CACTGTTCTT CGCCCTTTTC ATGAAGCATC GCCATGGCTA 
ATGAAGAAAG AATTAGACAC AATTGTTCCT CCTAATCTCC AGTCAAGGTC ATGGGAGAAA 
TTTCCCTCAG TTGAGAAAAA AACTCCAGGA CCTCCCTTTG GCACTCATAT TTGGAAGGGC 
CTTAACATTG AGTGCAAACA GCTAGTGAGT GCCCCAGCTC CTATACTCAA AAGCCCCAAT 
GTACTACAGA TACGCCAGTT CATCCAGCAT GAGGTCATAA ACGATGAGGT GAAACTGAAG 
CTGCAGATTT CCCTTAAGGA CTATATAAAC CATATTTTAA AGAAAGAAGA TGAGCTACAG 
GAAATGACAG TTAAAGATTC CAGAACTGAG GAGGAGAGAG GCAATGCTGC AGATCTCCTC 
AAGCTTGTTA TGTCTTTTCC TAAAATGGAG GAAACTACAA AAGGTCATGC TACTGAAGTT 
GCAGCCCACT TAGTTGATTT ATTCAAGCAA TCTTTGGCTG TTGCTCCAGA ATGTCCACCA 
GAAACATTTG ATACAGATTT CTTGGAAAAG AAAGGGAGGC ACATAGCAAG TGAAAAGCGC 
TGGAAAGAAA AGATAGATCA AAAGAGGAAG GAAATCGAAA AAACACACAA TTATCTAGAG 
GAAATCAGGA ACAGAACGGT ATCACCCTAC TTCAAATCAG AATTGAGCAA GACCTTTCAG 
TCTCAGTTTA ATTATCTAGA CGCCCTTTCC AAAAACTTGC CTCCTTTTAC AAAGAAAGTT 
GAAGATACAA ACAAAACAGC TGTTCAAAAT ACATCCAATC TTCAGCCAGA TTCCCAGCCA 
AATAAAGTTT CACAACAAGA ATCCTCAGAC ACACTTAGTC TTCCAAAATC AGAGGCTTTT 
GTGGATATGA AGGCGTTCGC TAGCCAGAAC CAAACTGAAA ATTTAACAAA ATCTACTGAA 
GAACCAGAAA CTTATGCCCC TGTGAAAATT CCAACCCAGT CATTGCTGCA GGATGTTGCA 
GGACAAGCAA GGAAAGAGAA AGTGAGGTTA CCTCGCTATT TGCTGAGTTC CAAACCCAAG 
TCTCAACCTC TCGAGAAGGT GCAAGATTCT GTTGGAGGAA GAGTGAGAAC ATCCTCGGTT 
GCTTCTGCTG CCATTAATAA CGCAAATTCA TCCCCTTTTG GATTTCATCT TCTCCCACCG 
TCAGTGAATT TTGGAGTGCT TGAAGAAGGG CACACCTATG AAACCACTGT GAAGCTCAAG 
AATGTTGGAG TGGACTTCTG CAGGTTTAGG GTGAAGCAGC CGCCACCCAG CACAGGATTG 
AAAGTGACTT ACAAACCTGG GCCTGTGGCA GCTGGTTTGC AGGCAGAACT GAAAGTGGAG 
TTATTTACCA TGGTTGTTGG AGAGGATGGT GCCAAGGGAT CAGCACACAT CTCTCACAAT 
ATTGAGATTA TGACTGAGCA TGATGTCCTG TTCTTACCTG TGGAAGCAAC AGTTTTAACA 
AGCAGCAATT ATGATAAGCG ATTAAAAGAT TTTCCCCAGG AAAAAGAAAA CCCCACGATC 
CAGAGGACTT CTACAATTTC TTCTGCTAAA CTTGGAGTCT TCATGTCTCG TAAAGTTTCT 
CCTTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023497098.1
CDS	67..6615
Translation	MAPKKEKSGSASANSKIWEPSLIAAHFNQNDWKGVIAFVVGNRIEDELLIGALTLAVQAPQRRLFSMVSWQDILQQINGTNALPGTASKKGKKPLGGSAPLYSEVTEDKFKTEKDKGKAKSPKEKKPSGTKVVKGKGKDQPEANTTVKKATQLKQRGEEDDAKRYIDDEPDDGAQHYIIVVGFNDPQLLAIMTELGIPISSVIKISSENYEPLQAHLAAVSQQQEVSLQPEDIEAEKLKNENTIKELEIFWKYLEPILNNEKPETNLFDVARLEYMVKEDGFPSDWSDSGMMLELGTDIFENIACLMYDSLDWKRQHRHYLESMQLINVPQVVNEKPILEAVAIAEAPQAAAPTPGKKKAQNEERQAPTSVPFVITTEADMRYYNDLLNPIPEEFISVPLILHCMLEQVVATEEDLVPPSLVEPPPRADGLDHRIAAHIVSILPSLCLTEKEKKNLQEIFLSEEENESKAVPKGPLLLNYHDTLAHKKYALKDPKNFDPVQIEQEMQSKLPLWEFLEFPLPPPWNSTKRLATIHELMHFCTNEVLSWNEVERAFKVFTFESLKLSTVDEKGKLKPARMMYGTDCEMFNIPWDNPARFAKQIRQQYITKMNTQEVKQKTDTETKDRILFVDQNMPVSVQDDESSKEPSDPSQADASNMQHSDSNNKKSLDPDDRELSEQDNSLKTQLQSEPLEQTTGNEIKDEAVTNVDPPEKKSKKMTVEAELEDIKKTEQRSLMDWSFTEHFKPKVLLQVLQEANQHYRCIDSYYHTQDNSLLLVFHNPMNLQRLHCEYWNVALHSNVGFRNYLELVAQSIQDWIIKEEAIYQESKMAEELSRSKLEQEVKSPASGKISSPSKIYSVKKSKSNKGMSKTELIDQEKEKEKEKEKIPFILEGSLKAWKEEQDRLAEEERLKEEKKAEKKSKDIGKKKGKEKVEREDSRVLKKKSFFKDKAKEEQIKISDVTEEPLQQPEPEIVYPFRGYNMGDIPIQISGTNCYLYPSDGGQIEVEKTTFEKGPTFIKVKVKKDKHNFIIHLKDPKKIVRNEKKEDDYSSEDEKDEGEEERNLEMKESDAENKAISKFGSFSATLENGICLSISYYGSSGMAPEDKDPNLEAMLNIPSALTPTIIPVVVTVPQGKGKGKTKGKEKPKDSIKEEEHPKEEEKKEEVEPEPVLQETVYVPTFQNLNVSCPSGLLVTFIGQESADHHVMDEEPTWDIMVRQSYPQKVKHYEFYKTVMPPVEQEASRVITSQGTVVKYMLDGSTQILFANGAVSSSPDSGPVCPPPEMPASPHNGDLMDSVSQQKSETAPSETVITKKGKGHKNQSSVPHTNEIHEPPQEVVQIVTPVEVHVGTWFTTTPDGNRIGTKGLEKIADLAPFLSFQATDPVNGTVMTTREDKVIIVEKKDGTRIVDHADGTRITTFYQVYEDHIMPPDDQEIPEHPQTLTRQVKCMRIESSHYATVITNCEDSSCCATFGDGTSIIAKPQGTYQVLPSNKGCLHIDRDCSAVYYHESSSDIYHPFQKREQLRASRYIMKHTSEVLCEVLDPEGNTFQVMADGSTSTLLPENNLEDDLNSKPEGYDSLSSTHLHKNHLQIYGEHVPRFFVIYADGSGVELLRDDDIEEYLSLAYGESTTVVLQEPVQEQPGALSITVLRPFHEASPWLMKKELDTIVPPNLQSRSWEKFPSVEKKTPGPPFGTHIWKGLNIECKQLVSAPAPILKSPNVLQIRQFIQHEVINDEVKLKLQISLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKGHATEVAAHLVDLFKQSLAVAPECPPETFDTDFLEKKGRHIASEKRWKEKIDQKRKEIEKTHNYLEEIRNRTVSPYFKSELSKTFQSQFNYLDALSKNLPPFTKKVEDTNKTAVQNTSNLQPDSQPNKVSQQESSDTLSLPKSEAFVDMKAFASQNQTENLTKSTEEPETYAPVKIPTQSLLQDVAGQARKEKVRLPRYLLSSKPKSQPLEKVQDSVGGRVRTSSVASAAINNANSSPFGFHLLPPSVNFGVLEEGHTYETTVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQAELKVELFTMVVGEDGAKGSAHISHNIEIMTEHDVLFLPVEATVLTSSNYDKRLKDFPQEKENPTIQRTSTISSAKLGVFMSRKVSPY

Target ORF information:

RefSeq Version	XM_023641330.1
Organism	Equus caballus(horse)
Definition	Equus caballus sperm associated antigen 17 (SPAG17), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023641330.1

ORF Insert Sequence:

ATGGCACCCA AGAAAGAGAA AAGCGGGTCG GCGAGCGCGA ATTCTAAGAT ATGGGAACCC 
TCGCTCATCG CTGCACACTT CAATCAGAAC GATTGGAAGG GCGTCATCGC TTTTGTAGTT 
GGAAACCGGA TTGAGGATGA GCTTCTCATT GGAGCCCTCA CCCTCGCTGT CCAGGCCCCT 
CAGCGTAGAC TCTTCAGTAT GGTATCATGG CAAGACATCC TCCAGCAGAT CAATGGAACA 
AATGCGCTTC CTGGAACTGC ATCTAAAAAG GGAAAGAAAC CTCTAGGCGG TAGCGCTCCC 
TTATATTCTG AGGTGACAGA AGACAAATTT AAGACTGAAA AGGATAAAGG GAAAGCGAAA 
TCTCCCAAGG AAAAGAAGCC CTCAGGTACC AAGGTTGTCA AAGGAAAGGG AAAGGATCAG 
CCTGAGGCAA ACACAACAGT GAAAAAGGCC ACTCAGTTAA AACAGAGGGG AGAAGAAGAC 
GACGCCAAAC GTTACATTGA TGATGAGCCA GATGATGGTG CCCAACATTA CATTATAGTT 
GTGGGCTTCA ACGATCCTCA GCTATTAGCG ATTATGACTG AGCTTGGCAT TCCCATAAGC 
AGCGTGATTA AAATATCTTC AGAGAATTAT GAACCTCTGC AGGCGCACCT GGCAGCAGTT 
AGCCAGCAGC AGGAAGTTTC TCTTCAGCCA GAAGATATAG AAGCTGAAAA ATTGAAGAAT 
GAGAATACCA TAAAAGAACT TGAAATTTTC TGGAAGTACT TGGAACCAAT CCTGAATAAT 
GAGAAACCTG AAACAAATCT CTTTGATGTT GCTCGTCTTG AATACATGGT CAAGGAAGAT 
GGCTTTCCTT CTGACTGGTC AGACAGTGGG ATGATGCTGG AATTGGGCAC CGACATTTTT 
GAAAATATTG CCTGTTTGAT GTACGACAGC CTGGATTGGA AAAGGCAGCA CCGGCACTAT 
TTAGAAAGCA TGCAGCTTAT TAATGTCCCA CAAGTGGTTA ACGAGAAGCC TATCTTAGAA 
GCTGTAGCCA TTGCTGAGGC TCCACAAGCT GCTGCTCCAA CCCCTGGGAA GAAGAAAGCA 
CAAAATGAAG AACGGCAAGC CCCAACATCA GTGCCTTTTG TCATCACAAC TGAAGCAGAC 
ATGAGATATT ACAATGATTT GCTGAATCCG ATTCCAGAAG AATTCATTTC TGTGCCCCTG 
ATACTGCACT GTATGCTGGA ACAGGTTGTA GCAACTGAAG AAGACCTTGT CCCACCCAGT 
CTGGTGGAGC CGCCTCCCAG AGCAGATGGG TTGGACCACA GAATTGCAGC TCACATTGTA 
TCCATCCTAC CCTCTCTCTG TCTCACGGAG AAGGAGAAAA AGAATCTCCA GGAAATATTT 
TTGTCTGAAG AAGAAAATGA AAGCAAAGCA GTGCCCAAAG GTCCTCTCCT ACTGAACTAT 
CACGACACTC TGGCCCACAA GAAGTATGCA CTGAAGGACC CAAAGAATTT CGACCCAGTT 
CAAATTGAGC AGGAGATGCA GTCCAAGTTG CCACTGTGGG AATTCCTTGA ATTCCCTCTG 
CCCCCGCCAT GGAACAGCAC TAAACGTCTA GCTACAATTC ATGAGCTCAT GCACTTTTGT 
ACAAATGAAG TCTTGAGCTG GAATGAAGTA GAACGAGCCT TCAAAGTGTT TACTTTTGAG 
AGCCTGAAAC TCTCTACAGT TGATGAAAAA GGGAAACTAA AGCCTGCTCG GATGATGTAT 
GGGACTGATT GTGAGATGTT CAACATACCA TGGGACAACC CTGCCAGATT TGCTAAACAG 
ATAAGGCAAC AATACATCAC GAAAATGAAC ACCCAAGAGG TCAAACAGAA AACAGATACT 
GAAACCAAAG ACAGAATTTT GTTTGTGGAT CAGAATATGC CAGTGTCTGT GCAAGATGAT 
GAGAGCAGTA AAGAACCTTC AGACCCTAGT CAGGCTGATG CTAGCAATAT GCAGCATTCT 
GACTCGAATA ATAAGAAATC CTTAGACCCT GATGATAGAG AGCTGTCAGA GCAGGACAAC 
AGCTTGAAGA CTCAGCTCCA ATCTGAGCCT TTGGAGCAGA CCACGGGCAA TGAGATCAAA 
GATGAAGCAG TCACAAATGT TGATCCTCCT GAAAAGAAAT CCAAGAAGAT GACCGTGGAA 
GCAGAGTTGG AGGACATAAA GAAAACAGAG CAACGCAGCC TAATGGATTG GAGTTTCACT 
GAACATTTTA AACCGAAAGT TCTACTGCAG GTTCTTCAAG AAGCCAATCA GCATTACAGA 
TGTATTGATT CTTATTACCA CACCCAAGAC AACTCTTTAC TATTAGTCTT TCATAATCCA 
ATGAATTTAC AACGTCTGCA TTGTGAATAT TGGAATGTTG CTCTTCACTC CAATGTTGGA 
TTCAGGAATT ATTTGGAACT TGTTGCACAG TCCATTCAAG ACTGGATCAT AAAAGAAGAA 
GCTATATATC AGGAATCTAA AATGGCTGAG GAACTCAGCA GGAGCAAGCT TGAGCAGGAA 
GTGAAATCTC CTGCTAGTGG CAAAATTTCT TCCCCTAGCA AAATTTATTC TGTTAAAAAA 
TCTAAAAGTA ACAAAGGAAT GAGCAAAACA GAGTTAATAG ATCAAGAAAA AGAAAAAGAG 
AAAGAGAAGG AAAAGATTCC TTTCATTTTA GAAGGCTCTC TCAAGGCATG GAAAGAAGAG 
CAAGATCGAT TAGCAGAAGA GGAACGCTTA AAGGAAGAAA AAAAAGCAGA GAAGAAGAGT 
AAGGACATTG GTAAAAAGAA AGGCAAGGAA AAGGTAGAGA GAGAAGATAG CAGGGTTTTG 
AAGAAAAAAT CTTTTTTCAA GGATAAAGCT AAAGAAGAGC AAATCAAGAT CTCAGACGTA 
ACAGAGGAGC CCCTTCAACA GCCAGAACCT GAGATAGTTT ACCCGTTTCG CGGATACAAT 
ATGGGAGATA TACCCATCCA AATCTCGGGA ACAAATTGTT ATCTGTATCC TTCAGATGGA 
GGGCAGATTG AAGTAGAAAA GACAACATTT GAAAAAGGTC CAACTTTTAT CAAAGTGAAA 
GTGAAAAAGG ACAAGCACAA TTTTATAATT CATTTAAAAG ATCCTAAGAA AATTGTGAGA 
AACGAAAAAA AAGAAGACGA TTATTCTTCA GAAGATGAGA AAGATGAAGG AGAGGAAGAA 
CGAAATCTTG AAATGAAAGA ATCAGATGCA GAGAATAAAG CTATCAGCAA GTTTGGATCT 
TTTTCTGCCA CCTTGGAAAA TGGAATCTGC CTCTCAATAA GTTACTACGG ATCAAGTGGA 
ATGGCGCCAG AGGATAAGGA TCCTAATTTG GAAGCAATGT TGAATATCCC GTCAGCACTC 
ACTCCAACAA TAATTCCTGT GGTAGTGACT GTTCCTCAAG GCAAAGGAAA AGGGAAAACC 
AAAGGCAAAG AAAAGCCCAA AGACTCTATT AAAGAAGAGG AACATCCAAA AGAAGAAGAG 
AAAAAGGAAG AAGTAGAACC AGAACCTGTT TTACAAGAGA CTGTTTATGT TCCCACCTTC 
CAAAACCTAA ATGTGTCTTG CCCCAGTGGG CTTCTAGTGA CCTTCATTGG CCAAGAGTCT 
GCAGATCACC ATGTTATGGA TGAGGAACCC ACTTGGGACA TCATGGTCCG ACAGAGCTAC 
CCCCAGAAGG TGAAGCACTA TGAGTTCTAC AAAACGGTGA TGCCACCAGT GGAGCAGGAG 
GCATCGAGAG TTATCACTAG TCAAGGCACT GTTGTCAAAT ACATGTTGGA TGGATCCACA 
CAGATTCTCT TTGCAAATGG CGCTGTGAGC AGTAGTCCCG ATTCAGGTCC TGTTTGTCCT 
CCTCCTGAAA TGCCAGCGAG CCCTCATAAC GGAGACTTGA TGGACAGTGT TTCTCAGCAA 
AAATCAGAAA CAGCACCGTC TGAGACTGTC ATCACAAAGA AAGGAAAAGG TCACAAAAAT 
CAGTCATCAG TGCCACATAC GAATGAAATC CATGAACCTC CCCAAGAGGT AGTTCAAATA 
GTAACACCTG TGGAGGTTCA TGTAGGCACC TGGTTTACAA CTACACCTGA CGGAAATCGG 
ATTGGCACCA AAGGATTAGA AAAAATAGCA GACTTGGCCC CATTCTTATC CTTTCAGGCC 
ACAGATCCTG TCAATGGAAC GGTTATGACA ACTCGAGAGG ACAAGGTGAT CATTGTTGAA 
AAGAAGGATG GTACTCGGAT CGTGGATCAT GCCGATGGTA CCAGAATCAC AACCTTTTAT 
CAAGTTTATG AAGATCATAT TATGCCTCCA GATGATCAAG AAATACCCGA ACATCCTCAA 
ACTCTCACCA GGCAGGTGAA GTGCATGCGC ATAGAAAGCT CACACTATGC CACTGTCATC 
ACCAACTGCG AGGACAGTAG CTGCTGTGCC ACTTTTGGAG ATGGGACGTC TATTATTGCA 
AAGCCACAGG GAACATACCA GGTGTTACCT TCAAACAAAG GCTGTCTTCA TATTGATAGA 
GACTGTTCAG CTGTATATTA CCATGAATCC AGCAGTGATA TATACCATCC TTTCCAAAAA 
CGTGAGCAGC TGAGAGCTAG CAGGTACATC ATGAAGCACA CGTCAGAGGT TCTCTGTGAA 
GTCCTGGATC CTGAGGGAAA CACCTTTCAG GTCATGGCTG ATGGTAGCAC ATCAACTCTG 
CTACCTGAAA ACAATTTGGA AGATGATTTA AACTCAAAAC CTGAAGGCTA TGATAGTTTA 
TCATCTACTC ACCTTCATAA AAATCACCTA CAAATATATG GTGAACATGT CCCCAGGTTT 
TTTGTTATCT ATGCCGATGG ATCAGGAGTA GAACTTCTTC GAGATGATGA CATAGAAGAA 
TACTTATCCT TGGCATATGG AGAATCAACC ACTGTAGTTC TTCAAGAGCC AGTACAAGAA 
CAGCCAGGCG CCCTGAGCAT CACTGTTCTT CGCCCTTTTC ATGAAGCATC GCCATGGCTA 
ATGAAGAAAG AATTAGACAC AATTGTTCCT CCTAATCTCC AGTCAAGGTC ATGGGAGAAA 
TTTCCCTCAG TTGAGAAAAA AACTCCAGGA CCTCCCTTTG GCACTCATAT TTGGAAGGGC 
CTTAACATTG AGTGCAAACA GCTAGTGAGT GCCCCAGCTC CTATACTCAA AAGCCCCAAT 
GTACTACAGA TACGCCAGTT CATCCAGCAT GAGGTCATAA ACGATGAGGT GAAACTGAAG 
CTGCAGATTT CCCTTAAGGA CTATATAAAC CATATTTTAA AGAAAGAAGA TGAGCTACAG 
GAAATGACAG TTAAAGATTC CAGAACTGAG GAGGAGAGAG GCAATGCTGC AGATCTCCTC 
AAGCTTGTTA TGTCTTTTCC TAAAATGGAG GAAACTACAA AAGGTCATGC TACTGAAGTT 
GCAGCCCACT TAGTTGATTT ATTCAAGCAA TCTTTGGCTG TTGCTCCAGA ATGTCCACCA 
GAAACATTTG ATACAGATTT CTTGGAAAAG AAAGGGAGGC ACATAGCAAG TGAAAAGCGC 
TGGAAAGAAA AGATAGATCA AAAGAGGAAG GAAATCGAAA AAACACACAA TTATCTAGAG 
GAAATCAGGA ACAGAACGGT ATCACCCTAC TTCAAATCAG AATTGAGCAA GACCTTTCAG 
TCTCAGTTTA ATTATCTAGA CGCCCTTTCC AAAAACTTGC CTCCTTTTAC AAAGAAAGTT 
GAAGATACAA ACAAAACAGC TGTTCAAAAT ACATCCAATC TTCAGCCAGA TTCCCAGCCA 
AATAAAGTTT CACAACAAGA ATCCTCAGAC ACACTTAGTC TTCCAAAATC AGAGGCTTTT 
GTGGATATGA AGGCGTTCGC TAGCCAGAAC CAAACTGAAA ATTTAACAAA ATCTACTGAA 
GAACCAGAAA CTTATGCCCC TGTGAAAATT CCAACCCAGT CATTGCTGCA GGATGTTGCA 
GGACAAGCAA GGAAAGAGAA AGTGAGGTTA CCTCGCTATT TGCTGAGTTC CAAACCCAAG 
TCTCAACCTC TCGAGAAGGT GCAAGATTCT GTTGGAGGAA GAGTGAGAAC ATCCTCGGTT 
GCTTCTGCTG CCATTAATAA CGCAAATTCA TCCCCTTTTG GATTTCATCT TCTCCCACCG 
TCAGTGAATT TTGGAGTGCT TGAAGAAGGG CACACCTATG AAACCACTGT GAAGCTCAAG 
AATGTTGGAG TGGACTTCTG CAGGTTTAGG GTGAAGCAGC CGCCACCCAG CACAGGATTG 
AAAGTGACTT ACAAACCTGG GCCTGTGGCA GCTGGTTTGC AGGCAGAACT GAAAGTGGAG 
TTATTTACCA TGGTTGTTGG AGAGGATGGT GCCAAGGGAT CAGCACACAT CTCTCACAAT 
ATTGAGATTA TGACTGAGCA TGATGTCCTG TTCTTACCTG TGGAAGCAAC AGTTTTAACA 
AGCAGCAATT ATGATAAGCG ATTAAAAGAT TTTCCCCAGG AAAAAGAAAA CCCCACGATC 
CAGAGGACTT CTACAATTTC TTCTGCTAAA CTTGGAGTCT TCATGTCTCG TAAAGTTTCT 
CCTTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_023641331.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023497099.1 Equus caballus sperm associated antigen 17 (SPAG17), transcript variant X4, mRNA.

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CloneID	OHb65451
Clone ID Related Accession (Same CDS sequence)	XM_023641331.1
Accession Version	XM_023641331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	sperm-associated antigen 17 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCA AGAAAGAGAA AAGCGGGTCG GCGAGCGCGA ATTCTAAGAT ATGGGAACCC 
TCGCTCATCG CTGCACACTT CAATCAGAAC GATTGGAAGG GCGTCATCGC TTTTGTAGTT 
GGAAACCGGA TTGAGGATGA GCTTCTCATT GGAGCCCTCA CCCTCGCTGT CCAGGCCCCT 
CAGCGTAGAC TCTTCAGTAT GGTATCATGG CAAGACATCC TCCAGCAGAT CAATGGAACA 
AATGCGCTTC CTGGAACTGC ATCTAAAAAG GGAAAGAAAC CTCTAGGCGG TAGCGCTCCC 
TTATATTCTG AGGTGTTAAT AGCAACAAAG GCAATTATGG ACAGTGGAGA GAAATTAACC 
TTACCGCTGA TAGGGAAACT CTTGAAATAT CAGCTTCTCC AGATTAAATA TAAGGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGGTGACA GAAGACAAAT TTAAGACTGA AAAGGATAAA 
GGGAAAGCGA AATCTCCCAA GGAAAAGAAG CCCTCAGGTA CCAAGGTTGT CAAAGGAAAG 
GGAAAGGATC AGCCTGAGGC AAACACAACA GTGAAAAAGG CCACTCAGTT AAAACAGAGG 
GGAGAAGAAG ACGACGCCAA ACGTTACATT GATATAGAAG CTGAAAAATT GAAGAATGAG 
AATACCATAA AAGAACTTGA AATTTTCTGG AAGTACTTGG AACCAATCCT GAATAATGAG 
AAACCTGAAA CAAATCTCTT TGATGTTGCT CGTCTTGAAT ACATGGTCAA GGAAGATGGC 
TTTCCTTCTG ACTGGTCAGA CAGTGGGATG ATGCTGGAAT TGGGCACCGA CATTTTTGAA 
AATATTGCCT GTTTGATGTA CGACAGCCTG GATTGGAAAA GGCAGCACCG GCACTATTTA 
GAAAGCATGC AGCTTATTAA TGTCCCACAA GTGGTTAACG AGAAGCCTAT CTTAGAAGCT 
GTAGCCATTG CTGAGGCTCC ACAAGCTGCT GCTCCAACCC CTGGGAAGAA GAAAGCACAA 
AATGAAGAAC GGCAAGCCCC AACATCAGTG CCTTTTGTCA TCACAACTGA AGCAGACATG 
AGATATTACA ATGATTTGCT GAATCCGATT CCAGAAGAAT TCATTTCTGT GCCCCTGATA 
CTGCACTGTA TGCTGGAACA GGTTGTAGCA ACTGAAGAAG ACCTTGTCCC ACCCAGTCTG 
GTGGAGCCGC CTCCCAGAGC AGATGGGTTG GACCACAGAA TTGCAGCTCA CATTGTATCC 
ATCCTACCCT CTCTCTGTCT CACGGAGAAG GAGAAAAAGA ATCTCCAGGA AATATTTTTG 
TCTGAAGAAG AAAATGAAAG CAAAGCAGTG CCCAAAGGTC CTCTCCTACT GAACTATCAC 
GACACTCTGG CCCACAAGAA GTATGCACTG AAGGACCCAA AGAATTTCGA CCCAGTTCAA 
ATTGAGCAGG AGATGCAGTC CAAGTTGCCA CTGTGGGAAT TCCTTGAATT CCCTCTGCCC 
CCGCCATGGA ACAGCACTAA ACGTCTAGCT ACAATTCATG AGCTCATGCA CTTTTGTACA 
AATGAAGTCT TGAGCTGGAA TGAAGTAGAA CGAGCCTTCA AAGTGTTTAC TTTTGAGAGC 
CTGAAACTCT CTACAGTTGA TGAAAAAGGG AAACTAAAGC CTGCTCGGAT GATGTATGGG 
ACTGATTGTG AGATGTTCAA CATACCATGG GACAACCCTG CCAGATTTGC TAAACAGATA 
AGGCAACAAT ACATCACGAA AATGAACACC CAAGAGGTCA AACAGAAAAC AGATACTGAA 
ACCAAAGACA GAATTTTGTT TGTGGATCAG AATATGCCAG TGTCTGTGCA AGATGATGAG 
AGCAGTAAAG AACCTTCAGA CCCTAGTCAG GCTGATGCTA GCAATATGCA GCATTCTGAC 
TCGAATAATA AGAAATCCTT AGACCCTGAT GATAGAGAGC TGTCAGAGCA GGACAACAGC 
TTGAAGACTC AGCTCCAATC TGAGCCTTTG GAGCAGACCA CGGGCAATGA GATCAAAGAT 
GAAGCAGTCA CAAATGTTGA TCCTCCTGAA AAGAAATCCA AGAAGATGAC CGTGGAAGCA 
GAGTTGGAGG ACATAAAGAA AACAGAGCAA CGCAGCCTAA TGGATTGGAG TTTCACTGAA 
CATTTTAAAC CGAAAGTTCT ACTGCAGGTT CTTCAAGAAG CCAATCAGCA TTACAGATGT 
ATTGATTCTT ATTACCACAC CCAAGACAAC TCTTTACTAT TAGTCTTTCA TAATCCAATG 
AATTTACAAC GTCTGCATTG TGAATATTGG AATGTTGCTC TTCACTCCAA TGTTGGATTC 
AGGAATTATT TGGAACTTGT TGCACAGTCC ATTCAAGACT GGATCATAAA AGAAGAAGCT 
ATATATCAGG AATCTAAAAT GGCTGAGGAA CTCAGCAGGA GCAAGCTTGA GCAGGAAGTG 
AAATCTCCTG CTAGTGGCAA AATTTCTTCC CCTAGCAAAA TTTATTCTGT TAAAAAATCT 
AAAAGTAACA AAGGAATGAG CAAAACAGAG TTAATAGATC AAGAAAAAGA AAAAGAGAAA 
GAGAAGGAAA AGATTCCTTT CATTTTAGAA GGCTCTCTCA AGGCATGGAA AGAAGAGCAA 
GATCGATTAG CAGAAGAGGA ACGCTTAAAG GAAGAAAAAA AAGCAGAGAA GAAGAGTAAG 
GACATTGGTA AAAAGAAAGG CAAGGAAAAG GTAGAGAGAG AAGATAGCAG GGTTTTGAAG 
AAAAAATCTT TTTTCAAGGA TAAAGCTAAA GAAGAGCAAA TCAAGATCTC AGACGTAACA 
GAGGAGCCCC TTCAACAGCC AGAACCTGAG ATAGTTTACC CGTTTCGCGG ATACAATATG 
GGAGATATAC CCATCCAAAT CTCGGGAACA AATTGTTATC TGTATCCTTC AGATGGAGGG 
CAGATTGAAG TAGAAAAGAC AACATTTGAA AAAGGTCCAA CTTTTATCAA AGTGAAAGTG 
AAAAAGGACA AGCACAATTT TATAATTCAT TTAAAAGATC CTAAGAAAAT TGTGAGAAAC 
GAAAAAAAAG AAGACGATTA TTCTTCAGAA GATGAGAAAG ATGAAGGAGA GGAAGAACGA 
AATCTTGAAA TGAAAGAATC AGATGCAGAG AATAAAGCTA TCAGCAAGTT TGGATCTTTT 
TCTGCCACCT TGGAAAATGG AATCTGCCTC TCAATAAGTT ACTACGGATC AAGTGGAATG 
GCGCCAGAGG ATAAGGATCC TAATTTGGAA GCAATGTTGA ATATCCCGTC AGCACTCACT 
CCAACAATAA TTCCTGTGGT AGTGACTGTT CCTCAAGGCA AAGGAAAAGG GAAAACCAAA 
GGCAAAGAAA AGCCCAAAGA CTCTATTAAA GAAGAGGAAC ATCCAAAAGA AGAAGAGAAA 
AAGGAAGAAG TAGAACCAGA ACCTGTTTTA CAAGAGACTG TTTATGTTCC CACCTTCCAA 
AACCTAAATG TGTCTTGCCC CAGTGGGCTT CTAGTGACCT TCATTGGCCA AGAGTCTGCA 
GATCACCATG TTATGGATGA GGAACCCACT TGGGACATCA TGGTCCGACA GAGCTACCCC 
CAGAAGGTGA AGCACTATGA GTTCTACAAA ACGGTGATGC CACCAGTGGA GCAGGAGGCA 
TCGAGAGTTA TCACTAGTCA AGGCACTGTT GTCAAATACA TGTTGGATGG ATCCACACAG 
ATTCTCTTTG CAAATGGCGC TGTGAGCAGT AGTCCCGATT CAGGTCCTGT TTGTCCTCCT 
CCTGAAATGC CAGCGAGCCC TCATAACGGA GACTTGATGG ACAGTGTTTC TCAGCAAAAA 
TCAGAAACAG CACCGTCTGA GACTGTCATC ACAAAGAAAG GAAAAGGTCA CAAAAATCAG 
TCATCAGTGC CACATACGAA TGAAATCCAT GAACCTCCCC AAGAGGTAGT TCAAATAGTA 
ACACCTGTGG AGGTTCATGT AGGCACCTGG TTTACAACTA CACCTGACGG AAATCGGATT 
GGCACCAAAG GATTAGAAAA AATAGCAGAC TTGGCCCCAT TCTTATCCTT TCAGGCCACA 
GATCCTGTCA ATGGAACGGT TATGACAACT CGAGAGGACA AGGTGATCAT TGTTGAAAAG 
AAGGATGGTA CTCGGATCGT GGATCATGCC GATGGTACCA GAATCACAAC CTTTTATCAA 
GTTTATGAAG ATCATATTAT GCCTCCAGAT GATCAAGAAA TACCCGAACA TCCTCAAACT 
CTCACCAGGC AGGTGAAGTG CATGCGCATA GAAAGCTCAC ACTATGCCAC TGTCATCACC 
AACTGCGAGG ACAGTAGCTG CTGTGCCACT TTTGGAGATG GGACGTCTAT TATTGCAAAG 
CCACAGGGAA CATACCAGGT GTTACCTTCA AACAAAGGCT GTCTTCATAT TGATAGAGAC 
TGTTCAGCTG TATATTACCA TGAATCCAGC AGTGATATAT ACCATCCTTT CCAAAAACGT 
GAGCAGCTGA GAGCTAGCAG GTACATCATG AAGCACACGT CAGAGGTTCT CTGTGAAGTC 
CTGGATCCTG AGGGAAACAC CTTTCAGGTC ATGGCTGATG GTAGCACATC AACTCTGCTA 
CCTGAAAACA ATTTGGAAGA TGATTTAAAC TCAAAACCTG AAGGCTATGA TAGTTTATCA 
TCTACTCACC TTCATAAAAA TCACCTACAA ATATATGGTG AACATGTCCC CAGGTTTTTT 
GTTATCTATG CCGATGGATC AGGAGTAGAA CTTCTTCGAG ATGATGACAT AGAAGAATAC 
TTATCCTTGG CATATGGAGA ATCAACCACT GTAGTTCTTC AAGAGCCAGT ACAAGAACAG 
CCAGGCGCCC TGAGCATCAC TGTTCTTCGC CCTTTTCATG AAGCATCGCC ATGGCTAATG 
AAGAAAGAAT TAGACACAAT TGTTCCTCCT AATCTCCAGT CAAGGTCATG GGAGAAATTT 
CCCTCAGTTG AGAAAAAAAC TCCAGGACCT CCCTTTGGCA CTCATATTTG GAAGGGCCTT 
AACATTGAGT GCAAACAGCT AGTGAGTGCC CCAGCTCCTA TACTCAAAAG CCCCAATGTA 
CTACAGATAC GCCAGTTCAT CCAGCATGAG GTCATAAACG ATGAGGTGAA ACTGAAGCTG 
CAGATTTCCC TTAAGGACTA TATAAACCAT ATTTTAAAGA AAGAAGATGA GCTACAGGAA 
ATGACAGTTA AAGATTCCAG AACTGAGGAG GAGAGAGGCA ATGCTGCAGA TCTCCTCAAG 
CTTGTTATGT CTTTTCCTAA AATGGAGGAA ACTACAAAAG GTCATGCTAC TGAAGTTGCA 
GCCCACTTAG TTGATTTATT CAAGCAATCT TTGGCTGTTG CTCCAGAATG TCCACCAGAA 
ACATTTGATA CAGATTTCTT GGAAAAGAAA GGGAGGCACA TAGCAAGTGA AAAGCGCTGG 
AAAGAAAAGA TAGATCAAAA GAGGAAGGAA ATCGAAAAAA CACACAATTA TCTAGAGGAA 
ATCAGGAACA GAACGGTATC ACCCTACTTC AAATCAGAAT TGAGCAAGAC CTTTCAGTCT 
CAGTTTAATT ATCTAGACGC CCTTTCCAAA AACTTGCCTC CTTTTACAAA GAAAGTTGAA 
GATACAAACA AAACAGCTGT TCAAAATACA TCCAATCTTC AGCCAGATTC CCAGCCAAAT 
AAAGTTTCAC AACAAGAATC CTCAGACACA CTTAGTCTTC CAAAATCAGA GGCTTTTGTG 
GATATGAAGG CGTTCGCTAG CCAGAACCAA ACTGAAAATT TAACAAAATC TACTGAAGAA 
CCAGAAACTT ATGCCCCTGT GAAAATTCCA ACCCAGTCAT TGCTGCAGGA TGTTGCAGGA 
CAAGCAAGGA AAGAGAAAGT GAGGTTACCT CGCTATTTGC TGAGTTCCAA ACCCAAGTCT 
CAACCTCTCG AGAAGGTGCA AGATTCTGTT GGAGGAAGAG TGAGAACATC CTCGGTTGCT 
TCTGCTGCCA TTAATAACGC AAATTCATCC CCTTTTGGAT TTCATCTTCT CCCACCGTCA 
GTGAATTTTG GAGTGCTTGA AGAAGGGCAC ACCTATGAAA CCACTGTGAA GCTCAAGAAT 
GTTGGAGTGG ACTTCTGCAG GTTTAGGGTG AAGCAGCCGC CACCCAGCAC AGGATTGAAA 
GTGACTTACA AACCTGGGCC TGTGGCAGCT GGTTTGCAGG CAGAACTGAA AGTGGAGTTA 
TTTACCATGG TTGTTGGAGA GGATGGTGCC AAGGGATCAG CACACATCTC TCACAATATT 
GAGATTATGA CTGAGCATGA TGTCCTGTTC TTACCTGTGG AAGCAACAGT TTTAACAAGC 
AGCAATTATG ATAAGCGATT AAAAGATTTT CCCCAGGAAA AAGAAAACCC CACGATCCAG 
AGGACTTCTA CAATTTCTTC TGCTAAACTT GGAGTCTTCA TGTCTCGTAA AGTTTCTCCT 
TATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023497099.1
CDS	67..6552
Translation	MAPKKEKSGSASANSKIWEPSLIAAHFNQNDWKGVIAFVVGNRIEDELLIGALTLAVQAPQRRLFSMVSWQDILQQINGTNALPGTASKKGKKPLGGSAPLYSEVLIATKAIMDSGEKLTLPLIGKLLKYQLLQIKYKDQQRRESEKKVTEDKFKTEKDKGKAKSPKEKKPSGTKVVKGKGKDQPEANTTVKKATQLKQRGEEDDAKRYIDIEAEKLKNENTIKELEIFWKYLEPILNNEKPETNLFDVARLEYMVKEDGFPSDWSDSGMMLELGTDIFENIACLMYDSLDWKRQHRHYLESMQLINVPQVVNEKPILEAVAIAEAPQAAAPTPGKKKAQNEERQAPTSVPFVITTEADMRYYNDLLNPIPEEFISVPLILHCMLEQVVATEEDLVPPSLVEPPPRADGLDHRIAAHIVSILPSLCLTEKEKKNLQEIFLSEEENESKAVPKGPLLLNYHDTLAHKKYALKDPKNFDPVQIEQEMQSKLPLWEFLEFPLPPPWNSTKRLATIHELMHFCTNEVLSWNEVERAFKVFTFESLKLSTVDEKGKLKPARMMYGTDCEMFNIPWDNPARFAKQIRQQYITKMNTQEVKQKTDTETKDRILFVDQNMPVSVQDDESSKEPSDPSQADASNMQHSDSNNKKSLDPDDRELSEQDNSLKTQLQSEPLEQTTGNEIKDEAVTNVDPPEKKSKKMTVEAELEDIKKTEQRSLMDWSFTEHFKPKVLLQVLQEANQHYRCIDSYYHTQDNSLLLVFHNPMNLQRLHCEYWNVALHSNVGFRNYLELVAQSIQDWIIKEEAIYQESKMAEELSRSKLEQEVKSPASGKISSPSKIYSVKKSKSNKGMSKTELIDQEKEKEKEKEKIPFILEGSLKAWKEEQDRLAEEERLKEEKKAEKKSKDIGKKKGKEKVEREDSRVLKKKSFFKDKAKEEQIKISDVTEEPLQQPEPEIVYPFRGYNMGDIPIQISGTNCYLYPSDGGQIEVEKTTFEKGPTFIKVKVKKDKHNFIIHLKDPKKIVRNEKKEDDYSSEDEKDEGEEERNLEMKESDAENKAISKFGSFSATLENGICLSISYYGSSGMAPEDKDPNLEAMLNIPSALTPTIIPVVVTVPQGKGKGKTKGKEKPKDSIKEEEHPKEEEKKEEVEPEPVLQETVYVPTFQNLNVSCPSGLLVTFIGQESADHHVMDEEPTWDIMVRQSYPQKVKHYEFYKTVMPPVEQEASRVITSQGTVVKYMLDGSTQILFANGAVSSSPDSGPVCPPPEMPASPHNGDLMDSVSQQKSETAPSETVITKKGKGHKNQSSVPHTNEIHEPPQEVVQIVTPVEVHVGTWFTTTPDGNRIGTKGLEKIADLAPFLSFQATDPVNGTVMTTREDKVIIVEKKDGTRIVDHADGTRITTFYQVYEDHIMPPDDQEIPEHPQTLTRQVKCMRIESSHYATVITNCEDSSCCATFGDGTSIIAKPQGTYQVLPSNKGCLHIDRDCSAVYYHESSSDIYHPFQKREQLRASRYIMKHTSEVLCEVLDPEGNTFQVMADGSTSTLLPENNLEDDLNSKPEGYDSLSSTHLHKNHLQIYGEHVPRFFVIYADGSGVELLRDDDIEEYLSLAYGESTTVVLQEPVQEQPGALSITVLRPFHEASPWLMKKELDTIVPPNLQSRSWEKFPSVEKKTPGPPFGTHIWKGLNIECKQLVSAPAPILKSPNVLQIRQFIQHEVINDEVKLKLQISLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKGHATEVAAHLVDLFKQSLAVAPECPPETFDTDFLEKKGRHIASEKRWKEKIDQKRKEIEKTHNYLEEIRNRTVSPYFKSELSKTFQSQFNYLDALSKNLPPFTKKVEDTNKTAVQNTSNLQPDSQPNKVSQQESSDTLSLPKSEAFVDMKAFASQNQTENLTKSTEEPETYAPVKIPTQSLLQDVAGQARKEKVRLPRYLLSSKPKSQPLEKVQDSVGGRVRTSSVASAAINNANSSPFGFHLLPPSVNFGVLEEGHTYETTVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQAELKVELFTMVVGEDGAKGSAHISHNIEIMTEHDVLFLPVEATVLTSSNYDKRLKDFPQEKENPTIQRTSTISSAKLGVFMSRKVSPY

Target ORF information:

RefSeq Version	XM_023641331.1
Organism	Equus caballus(horse)
Definition	Equus caballus sperm associated antigen 17 (SPAG17), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023641331.1

ORF Insert Sequence:

ATGGCACCCA AGAAAGAGAA AAGCGGGTCG GCGAGCGCGA ATTCTAAGAT ATGGGAACCC 
TCGCTCATCG CTGCACACTT CAATCAGAAC GATTGGAAGG GCGTCATCGC TTTTGTAGTT 
GGAAACCGGA TTGAGGATGA GCTTCTCATT GGAGCCCTCA CCCTCGCTGT CCAGGCCCCT 
CAGCGTAGAC TCTTCAGTAT GGTATCATGG CAAGACATCC TCCAGCAGAT CAATGGAACA 
AATGCGCTTC CTGGAACTGC ATCTAAAAAG GGAAAGAAAC CTCTAGGCGG TAGCGCTCCC 
TTATATTCTG AGGTGTTAAT AGCAACAAAG GCAATTATGG ACAGTGGAGA GAAATTAACC 
TTACCGCTGA TAGGGAAACT CTTGAAATAT CAGCTTCTCC AGATTAAATA TAAGGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGGTGACA GAAGACAAAT TTAAGACTGA AAAGGATAAA 
GGGAAAGCGA AATCTCCCAA GGAAAAGAAG CCCTCAGGTA CCAAGGTTGT CAAAGGAAAG 
GGAAAGGATC AGCCTGAGGC AAACACAACA GTGAAAAAGG CCACTCAGTT AAAACAGAGG 
GGAGAAGAAG ACGACGCCAA ACGTTACATT GATATAGAAG CTGAAAAATT GAAGAATGAG 
AATACCATAA AAGAACTTGA AATTTTCTGG AAGTACTTGG AACCAATCCT GAATAATGAG 
AAACCTGAAA CAAATCTCTT TGATGTTGCT CGTCTTGAAT ACATGGTCAA GGAAGATGGC 
TTTCCTTCTG ACTGGTCAGA CAGTGGGATG ATGCTGGAAT TGGGCACCGA CATTTTTGAA 
AATATTGCCT GTTTGATGTA CGACAGCCTG GATTGGAAAA GGCAGCACCG GCACTATTTA 
GAAAGCATGC AGCTTATTAA TGTCCCACAA GTGGTTAACG AGAAGCCTAT CTTAGAAGCT 
GTAGCCATTG CTGAGGCTCC ACAAGCTGCT GCTCCAACCC CTGGGAAGAA GAAAGCACAA 
AATGAAGAAC GGCAAGCCCC AACATCAGTG CCTTTTGTCA TCACAACTGA AGCAGACATG 
AGATATTACA ATGATTTGCT GAATCCGATT CCAGAAGAAT TCATTTCTGT GCCCCTGATA 
CTGCACTGTA TGCTGGAACA GGTTGTAGCA ACTGAAGAAG ACCTTGTCCC ACCCAGTCTG 
GTGGAGCCGC CTCCCAGAGC AGATGGGTTG GACCACAGAA TTGCAGCTCA CATTGTATCC 
ATCCTACCCT CTCTCTGTCT CACGGAGAAG GAGAAAAAGA ATCTCCAGGA AATATTTTTG 
TCTGAAGAAG AAAATGAAAG CAAAGCAGTG CCCAAAGGTC CTCTCCTACT GAACTATCAC 
GACACTCTGG CCCACAAGAA GTATGCACTG AAGGACCCAA AGAATTTCGA CCCAGTTCAA 
ATTGAGCAGG AGATGCAGTC CAAGTTGCCA CTGTGGGAAT TCCTTGAATT CCCTCTGCCC 
CCGCCATGGA ACAGCACTAA ACGTCTAGCT ACAATTCATG AGCTCATGCA CTTTTGTACA 
AATGAAGTCT TGAGCTGGAA TGAAGTAGAA CGAGCCTTCA AAGTGTTTAC TTTTGAGAGC 
CTGAAACTCT CTACAGTTGA TGAAAAAGGG AAACTAAAGC CTGCTCGGAT GATGTATGGG 
ACTGATTGTG AGATGTTCAA CATACCATGG GACAACCCTG CCAGATTTGC TAAACAGATA 
AGGCAACAAT ACATCACGAA AATGAACACC CAAGAGGTCA AACAGAAAAC AGATACTGAA 
ACCAAAGACA GAATTTTGTT TGTGGATCAG AATATGCCAG TGTCTGTGCA AGATGATGAG 
AGCAGTAAAG AACCTTCAGA CCCTAGTCAG GCTGATGCTA GCAATATGCA GCATTCTGAC 
TCGAATAATA AGAAATCCTT AGACCCTGAT GATAGAGAGC TGTCAGAGCA GGACAACAGC 
TTGAAGACTC AGCTCCAATC TGAGCCTTTG GAGCAGACCA CGGGCAATGA GATCAAAGAT 
GAAGCAGTCA CAAATGTTGA TCCTCCTGAA AAGAAATCCA AGAAGATGAC CGTGGAAGCA 
GAGTTGGAGG ACATAAAGAA AACAGAGCAA CGCAGCCTAA TGGATTGGAG TTTCACTGAA 
CATTTTAAAC CGAAAGTTCT ACTGCAGGTT CTTCAAGAAG CCAATCAGCA TTACAGATGT 
ATTGATTCTT ATTACCACAC CCAAGACAAC TCTTTACTAT TAGTCTTTCA TAATCCAATG 
AATTTACAAC GTCTGCATTG TGAATATTGG AATGTTGCTC TTCACTCCAA TGTTGGATTC 
AGGAATTATT TGGAACTTGT TGCACAGTCC ATTCAAGACT GGATCATAAA AGAAGAAGCT 
ATATATCAGG AATCTAAAAT GGCTGAGGAA CTCAGCAGGA GCAAGCTTGA GCAGGAAGTG 
AAATCTCCTG CTAGTGGCAA AATTTCTTCC CCTAGCAAAA TTTATTCTGT TAAAAAATCT 
AAAAGTAACA AAGGAATGAG CAAAACAGAG TTAATAGATC AAGAAAAAGA AAAAGAGAAA 
GAGAAGGAAA AGATTCCTTT CATTTTAGAA GGCTCTCTCA AGGCATGGAA AGAAGAGCAA 
GATCGATTAG CAGAAGAGGA ACGCTTAAAG GAAGAAAAAA AAGCAGAGAA GAAGAGTAAG 
GACATTGGTA AAAAGAAAGG CAAGGAAAAG GTAGAGAGAG AAGATAGCAG GGTTTTGAAG 
AAAAAATCTT TTTTCAAGGA TAAAGCTAAA GAAGAGCAAA TCAAGATCTC AGACGTAACA 
GAGGAGCCCC TTCAACAGCC AGAACCTGAG ATAGTTTACC CGTTTCGCGG ATACAATATG 
GGAGATATAC CCATCCAAAT CTCGGGAACA AATTGTTATC TGTATCCTTC AGATGGAGGG 
CAGATTGAAG TAGAAAAGAC AACATTTGAA AAAGGTCCAA CTTTTATCAA AGTGAAAGTG 
AAAAAGGACA AGCACAATTT TATAATTCAT TTAAAAGATC CTAAGAAAAT TGTGAGAAAC 
GAAAAAAAAG AAGACGATTA TTCTTCAGAA GATGAGAAAG ATGAAGGAGA GGAAGAACGA 
AATCTTGAAA TGAAAGAATC AGATGCAGAG AATAAAGCTA TCAGCAAGTT TGGATCTTTT 
TCTGCCACCT TGGAAAATGG AATCTGCCTC TCAATAAGTT ACTACGGATC AAGTGGAATG 
GCGCCAGAGG ATAAGGATCC TAATTTGGAA GCAATGTTGA ATATCCCGTC AGCACTCACT 
CCAACAATAA TTCCTGTGGT AGTGACTGTT CCTCAAGGCA AAGGAAAAGG GAAAACCAAA 
GGCAAAGAAA AGCCCAAAGA CTCTATTAAA GAAGAGGAAC ATCCAAAAGA AGAAGAGAAA 
AAGGAAGAAG TAGAACCAGA ACCTGTTTTA CAAGAGACTG TTTATGTTCC CACCTTCCAA 
AACCTAAATG TGTCTTGCCC CAGTGGGCTT CTAGTGACCT TCATTGGCCA AGAGTCTGCA 
GATCACCATG TTATGGATGA GGAACCCACT TGGGACATCA TGGTCCGACA GAGCTACCCC 
CAGAAGGTGA AGCACTATGA GTTCTACAAA ACGGTGATGC CACCAGTGGA GCAGGAGGCA 
TCGAGAGTTA TCACTAGTCA AGGCACTGTT GTCAAATACA TGTTGGATGG ATCCACACAG 
ATTCTCTTTG CAAATGGCGC TGTGAGCAGT AGTCCCGATT CAGGTCCTGT TTGTCCTCCT 
CCTGAAATGC CAGCGAGCCC TCATAACGGA GACTTGATGG ACAGTGTTTC TCAGCAAAAA 
TCAGAAACAG CACCGTCTGA GACTGTCATC ACAAAGAAAG GAAAAGGTCA CAAAAATCAG 
TCATCAGTGC CACATACGAA TGAAATCCAT GAACCTCCCC AAGAGGTAGT TCAAATAGTA 
ACACCTGTGG AGGTTCATGT AGGCACCTGG TTTACAACTA CACCTGACGG AAATCGGATT 
GGCACCAAAG GATTAGAAAA AATAGCAGAC TTGGCCCCAT TCTTATCCTT TCAGGCCACA 
GATCCTGTCA ATGGAACGGT TATGACAACT CGAGAGGACA AGGTGATCAT TGTTGAAAAG 
AAGGATGGTA CTCGGATCGT GGATCATGCC GATGGTACCA GAATCACAAC CTTTTATCAA 
GTTTATGAAG ATCATATTAT GCCTCCAGAT GATCAAGAAA TACCCGAACA TCCTCAAACT 
CTCACCAGGC AGGTGAAGTG CATGCGCATA GAAAGCTCAC ACTATGCCAC TGTCATCACC 
AACTGCGAGG ACAGTAGCTG CTGTGCCACT TTTGGAGATG GGACGTCTAT TATTGCAAAG 
CCACAGGGAA CATACCAGGT GTTACCTTCA AACAAAGGCT GTCTTCATAT TGATAGAGAC 
TGTTCAGCTG TATATTACCA TGAATCCAGC AGTGATATAT ACCATCCTTT CCAAAAACGT 
GAGCAGCTGA GAGCTAGCAG GTACATCATG AAGCACACGT CAGAGGTTCT CTGTGAAGTC 
CTGGATCCTG AGGGAAACAC CTTTCAGGTC ATGGCTGATG GTAGCACATC AACTCTGCTA 
CCTGAAAACA ATTTGGAAGA TGATTTAAAC TCAAAACCTG AAGGCTATGA TAGTTTATCA 
TCTACTCACC TTCATAAAAA TCACCTACAA ATATATGGTG AACATGTCCC CAGGTTTTTT 
GTTATCTATG CCGATGGATC AGGAGTAGAA CTTCTTCGAG ATGATGACAT AGAAGAATAC 
TTATCCTTGG CATATGGAGA ATCAACCACT GTAGTTCTTC AAGAGCCAGT ACAAGAACAG 
CCAGGCGCCC TGAGCATCAC TGTTCTTCGC CCTTTTCATG AAGCATCGCC ATGGCTAATG 
AAGAAAGAAT TAGACACAAT TGTTCCTCCT AATCTCCAGT CAAGGTCATG GGAGAAATTT 
CCCTCAGTTG AGAAAAAAAC TCCAGGACCT CCCTTTGGCA CTCATATTTG GAAGGGCCTT 
AACATTGAGT GCAAACAGCT AGTGAGTGCC CCAGCTCCTA TACTCAAAAG CCCCAATGTA 
CTACAGATAC GCCAGTTCAT CCAGCATGAG GTCATAAACG ATGAGGTGAA ACTGAAGCTG 
CAGATTTCCC TTAAGGACTA TATAAACCAT ATTTTAAAGA AAGAAGATGA GCTACAGGAA 
ATGACAGTTA AAGATTCCAG AACTGAGGAG GAGAGAGGCA ATGCTGCAGA TCTCCTCAAG 
CTTGTTATGT CTTTTCCTAA AATGGAGGAA ACTACAAAAG GTCATGCTAC TGAAGTTGCA 
GCCCACTTAG TTGATTTATT CAAGCAATCT TTGGCTGTTG CTCCAGAATG TCCACCAGAA 
ACATTTGATA CAGATTTCTT GGAAAAGAAA GGGAGGCACA TAGCAAGTGA AAAGCGCTGG 
AAAGAAAAGA TAGATCAAAA GAGGAAGGAA ATCGAAAAAA CACACAATTA TCTAGAGGAA 
ATCAGGAACA GAACGGTATC ACCCTACTTC AAATCAGAAT TGAGCAAGAC CTTTCAGTCT 
CAGTTTAATT ATCTAGACGC CCTTTCCAAA AACTTGCCTC CTTTTACAAA GAAAGTTGAA 
GATACAAACA AAACAGCTGT TCAAAATACA TCCAATCTTC AGCCAGATTC CCAGCCAAAT 
AAAGTTTCAC AACAAGAATC CTCAGACACA CTTAGTCTTC CAAAATCAGA GGCTTTTGTG 
GATATGAAGG CGTTCGCTAG CCAGAACCAA ACTGAAAATT TAACAAAATC TACTGAAGAA 
CCAGAAACTT ATGCCCCTGT GAAAATTCCA ACCCAGTCAT TGCTGCAGGA TGTTGCAGGA 
CAAGCAAGGA AAGAGAAAGT GAGGTTACCT CGCTATTTGC TGAGTTCCAA ACCCAAGTCT 
CAACCTCTCG AGAAGGTGCA AGATTCTGTT GGAGGAAGAG TGAGAACATC CTCGGTTGCT 
TCTGCTGCCA TTAATAACGC AAATTCATCC CCTTTTGGAT TTCATCTTCT CCCACCGTCA 
GTGAATTTTG GAGTGCTTGA AGAAGGGCAC ACCTATGAAA CCACTGTGAA GCTCAAGAAT 
GTTGGAGTGG ACTTCTGCAG GTTTAGGGTG AAGCAGCCGC CACCCAGCAC AGGATTGAAA 
GTGACTTACA AACCTGGGCC TGTGGCAGCT GGTTTGCAGG CAGAACTGAA AGTGGAGTTA 
TTTACCATGG TTGTTGGAGA GGATGGTGCC AAGGGATCAG CACACATCTC TCACAATATT 
GAGATTATGA CTGAGCATGA TGTCCTGTTC TTACCTGTGG AAGCAACAGT TTTAACAAGC 
AGCAATTATG ATAAGCGATT AAAAGATTTT CCCCAGGAAA AAGAAAACCC CACGATCCAG 
AGGACTTCTA CAATTTCTTC TGCTAAACTT GGAGTCTTCA TGTCTCGTAA AGTTTCTCCT 
TATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_023641332.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023497100.1 Equus caballus sperm associated antigen 17 (SPAG17), transcript variant X5, mRNA.

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CloneID	OHb65452
Clone ID Related Accession (Same CDS sequence)	XM_023641332.1
Accession Version	XM_023641332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	sperm-associated antigen 17 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCA AGAAAGAGAA AAGCGGGTCG GCGAGCGCGA ATTCTAAGAT ATGGGAACCC 
TCGCTCATCG CTGCACACTT CAATCAGAAC GATTGGAAGG GCGTCATCGC TTTTGTAGTT 
GGAAACCGGA TTGAGGATGA GCTTCTCATT GGAGCCCTCA CCCTCGCTGT CCAGGCCCCT 
CAGCGTAGAC TCTTCAGTAT GGTATCATGG CAAGACATCC TCCAGCAGAT CAATGGAACA 
AATGCGCTTC CTGGAACTGC ATCTAAAAAG GGAAAGAAAC CTCTAGGCGG TAGCGCTCCC 
TTATATTCTG AGGTGTTAAT AGCAACAAAG GCAATTATGG ACAGTGGAGA GAAATTAACC 
TTACCGCTGA TAGGGAAACT CTTGAAATAT CAGCTTCTCC AGATTAAATA TAAGGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGGTGACA GAAGACAAAT TTAAGACTGA AAAGGATAAA 
GGGAAAGCGA AATCTCCCAA GGAAAAGAAG CCCTCAGGTA CCAAGGTTGT CAAAGGAAAG 
GGAAAGGATC AGCCTGAGGC AAACACAACA GTGAAAAAGG CCACTCAGTT AAAACAGAGG 
GGAGAAGAAG ACGACGCCAA ACGTTACATT GATGATGAGC CAGATGATGG TGCCCAACAT 
TACATTATAG TTGTGGGCTT CAACGATCCT CAGCTATTAG CGATTATGAC TGAGCTTGGC 
ATTCCCATAA GCAGCGTGAT TAAAATATCT TCAGAGAATT ATGAACCTCT GCAGGCGCAC 
CTGGCAGCAG TTAGCCAGCA GCAGGAAGTT TCTCTTCAGC CAGAAGATAT AGAAGCTGAA 
AAATTGAAGA ATGAGAATAC CATAAAAGAA CTTGAAATTT TCTGGAAGTA CTTGGAACCA 
ATCCTGAATA ATGAGAAACC TGAAACAAAT CTCTTTGATG TTGCTCGTCT TGAATACATG 
GTCAAGGAAG ATGGCTTTCC TTCTGACTGG TCAGACAGTG GGATGATGCT GGAATTGGGC 
ACCGACATTT TTGAAAATAT TGCCTGTTTG ATGTACGACA GCCTGGATTG GAAAAGGCAG 
CACCGGCACT ATTTAGAAAG CATGCAGCTT ATTAATGTCC CACAAGTGGT TAACGAGAAG 
CCTATCTTAG AAGCTGTAGC CATTGCTGAG GCTCCACAAG CTGCTGCTCC AACCCCTGGG 
AAGAAGAAAG CACAAAATGA AGAACGGCAA GCCCCAACAT CAGTGCCTTT TGTCATCACA 
ACTGAAGCAG ACATGAGATA TTACAATGAT TTGCTGAATC CGATTCCAGA AGAATTCATT 
TCTGTGCCCC TGATACTGCA CTGTATGCTG GAACAGGTTG TAGCAACTGA AGAAGACCTT 
GTCCCACCCA GTCTGGTGGA GCCGCCTCCC AGAGCAGATG GGTTGGACCA CAGAATTGCA 
GCTCACATTG TATCCATCCT ACCCTCTCTC TGTCTCACGG AGAAGGAGAA AAAGAATCTC 
CAGGAAATAT TTTTGTCTGA AGAAGAAAAT GAAAGCAAAG CAGTGCCCAA AGGTCCTCTC 
CTACTGAACT ATCACGACAC TCTGGCCCAC AAGAAGTATG CACTGAAGGA CCCAAAGAAT 
TTCGACCCAG TTCAAATTGA GCAGGAGATG CAGTCCAAGT TGCCACTGTG GGAATTCCTT 
GAATTCCCTC TGCCCCCGCC ATGGAACAGC ACTAAACGTC TAGCTACAAT TCATGAGCTC 
ATGCACTTTT GTACAAATGA AGTCTTGAGC TGGAATGAAG TAGAACGAGC CTTCAAAGTG 
TTTACTTTTG AGAGCCTGAA ACTCTCTACA GTTGATGAAA AAGGGAAACT AAAGCCTGCT 
CGGATGATGT ATGGGACTGA TTGTGAGATG TTCAACATAC CATGGGACAA CCCTGCCAGA 
TTTGCTAAAC AGATAAGGCA ACAATACATC ACGAAAATGA ACACCCAAGA GGTCAAACAG 
AAAACAGATA CTGAAACCAA AGACAGAATT TTGTTTGTGG ATCAGAATAT GCCAGTGTCT 
GTGCAAGATG ATGAGAGCAG TAAAGAACCT TCAGACCCTA GTCAGGCTGA TGCTAGCAAT 
ATGCAGCATT CTGACTCGAA TAATAAGAAA TCCTTAGACC CTGATGATAG AGAGCTGTCA 
GAGCAGGACA ACAGCTTGAA GACTCAGCTC CAATCTGAGC CTTTGGAGCA GACCACGGGC 
AATGAGATCA AAGATGAAGC AGTCACAAAT GTTGATCCTC CTGAAAAGAA ATCCAAGAAG 
ATGACCGTGG AAGCAGAGTT GGAGGACATA AAGAAAACAG AGCAACGCAG CCTAATGGAT 
TGGAGTTTCA CTGAACATTT TAAACCGAAA GTTCTACTGC AGGTTCTTCA AGAAGCCAAT 
CAGCATTACA GATGTATTGA TTCTTATTAC CACACCCAAG ACAACTCTTT ACTATTAGTC 
TTTCATAATC CAATGAATTT ACAACGTCTG CATTGTGAAT ATTGGAATGT TGCTCTTCAC 
TCCAATGTTG GATTCAGGAA TTATTTGGAA CTTGTTGCAC AGTCCATTCA AGACTGGATC 
ATAAAAGAAG AAGCTATATA TCAGGAATCT AAAATGGCTG AGGAACTCAG CAGGAGCAAG 
CTTGAGCAGG AAGTGAAATC TCCTGCTAGT GGCAAAATTT CTTCCCCTAG CAAAATTTAT 
TCTGTTAAAA AATCTAAAAG TAACAAAGGA ATGAGCAAAA CAGAGTTAAT AGATCAAGAA 
AAAGAAAAAG AGAAAGAGAA GGAAAAGATT CCTTTCATTT TAGAAGGCTC TCTCAAGGCA 
TGGAAAGAAG AGCAAGATCG ATTAGCAGAA GAGGAACGCT TAAAGGAAGA AAAAAAAGCA 
GAGAAGAAGA GTAAGGACAT TGGTAAAAAG AAAGGCAAGG AAAAGGTAGA GAGAGAAGAT 
AGCAGGGTTT TGAAGAAAAA ATCTTTTTTC AAGGATAAAG CTAAAGAAGA GCAAATCAAG 
ATCTCAGACG TAACAGAGGA GCCCCTTCAA CAGCCAGAAC CTGAGATAGT TTACCCGTTT 
CGCGGATACA ATATGGGAGA TATACCCATC CAAATCTCGG GAACAAATTG TTATCTGTAT 
CCTTCAGATG GAGGGCAGAT TGAAGTAGAA AAGACAACAT TTGAAAAAGG TCCAACTTTT 
ATCAAAGTGA AAGTGAAAAA GGACAAGCAC AATTTTATAA TTCATTTAAA AGATCCTAAG 
AAAATTGTGA GAAACGAAAA AAAAGAAGAC GATTATTCTT CAGAAGATGA GAAAGATGAA 
GGAGAGGAAG AACGAAATCT TGAAATGAAA GAATCAGATG CAGAGAATAA AGCTATCAGC 
AAGTTTGGAT CTTTTTCTGC CACCTTGGAA AATGGAATCT GCCTCTCAAT AAGTTACTAC 
GGATCAAGTG GAATGGCGCC AGAGGATAAG GATCCTAATT TGGAAGCAAT GTTGAATATC 
CCGTCAGCAC TCACTCCAAC AATAATTCCT GTGGTAGTGA CTGTTCCTCA AGGCAAAGGA 
AAAGGGAAAA CCAAAGGCAA AGAAAAGCCC AAAGACTCTA TTAAAGAAGA GGAACATCCA 
AAAGAAGAAG AGAAAAAGGA AGAAGTAGAA CCAGAACCTG TTTTACAAGA GACTGTTTAT 
GTTCCCACCT TCCAAAACCT AAATGTGTCT TGCCCCAGTG GGCTTCTAGT GACCTTCATT 
GGCCAAGAGT CTGCAGATCA CCATGTTATG GATGAGGAAC CCACTTGGGA CATCATGGTC 
CGACAGAGCT ACCCCCAGAA GGTGAAGCAC TATGAGTTCT ACAAAACGGT GATGCCACCA 
GTGGAGCAGG AGGCATCGAG AGTTATCACT AGTCAAGGCA CTGTTGTCAA ATACATGTTG 
GATGGATCCA CACAGATTCT CTTTGCAAAT GGCGCTGTGA GCAGTAGTCC CGATTCAGGT 
CCTGTTTGTC CTCCTCCTGA AATGCCAGCG AGCCCTCATA ACGGAGACTT GATGGACAGT 
GTTTCTCAGC AAAAATCAGA AACAGCACCG TCTGAGACTG TCATCACAAA GAAAGGAAAA 
GGTCACAAAA ATCAGTCATC AGTGCCACAT ACGAATGAAA TCCATGAACC TCCCCAAGAG 
GTAGTTCAAA TAGTAACACC TGTGGAGGTT CATGTAGGCA CCTGGTTTAC AACTACACCT 
GACGGAAATC GGATTGGCAC CAAAGGATTA GAAAAAATAG CAGACTTGGC CCCATTCTTA 
TCCTTTCAGG CCACAGATCC TGTCAATGGA ACGGTTATGA CAACTCGAGA GGACAAGGTG 
ATCATTGTTG AAAAGAAGGA TGGTACTCGG ATCGTGGATC ATGCCGATGG TACCAGAATC 
ACAACCTTTT ATCAAGTTTA TGAAGATCAT ATTATGCCTC CAGATGATCA AGAAATACCC 
GAACATCCTC AAACTCTCAC CAGGCAGGTG AAGTGCATGC GCATAGAAAG CTCACACTAT 
GCCACTGTCA TCACCAACTG CGAGGACAGT AGCTGCTGTG CCACTTTTGG AGATGGGACG 
TCTATTATTG CAAAGCCACA GGGAACATAC CAGGTGTTAC CTTCAAACAA AGGCTGTCTT 
CATATTGATA GAGACTGTTC AGCTGTATAT TACCATGAAT CCAGCAGTGA TATATACCAT 
CCTTTCCAAA AACGTGAGCA GCTGAGAGCT AGCAGGTACA TCATGAAGCA CACGTCAGAG 
GTTCTCTGTG AAGTCCTGGA TCCTGAGGGA AACACCTTTC AGGTCATGGC TGATGGTAGC 
ACATCAACTC TGCTACCTGA AAACAATTTG GAAGATGATT TAAACTCAAA ACCTGAAGGC 
TATGATAGTT TATCATCTAC TCACCTTCAT AAAAATCACC TACAAATATA TGGTGAACAT 
GTCCCCAGGT TTTTTGTTAT CTATGCCGAT GGATCAGGAG TAGAACTTCT TCGAGATGAT 
GACATAGAAG AATACTTATC CTTGGCATAT GGAGAATCAA CCACTGTAGT TCTTCAAGAG 
CCAGTACAAG AACAGCCAGG CGCCCTGAGC ATCACTGTTC TTCGCCCTTT TCATGAAGCA 
TCGCCATGGC TAATGAAGAA AGAATTAGAC ACAATTGTTC CTCCTAATCT CCAGTCAAGG 
TCATGGGAGA AATTTCCCTC AGTTGAGAAA AAAACTCCAG GACCTCCCTT TGGCACTCAT 
ATTTGGAAGG GCCTTAACAT TGAGTGCAAA CAGCTAGTGA GTGCCCCAGC TCCTATACTC 
AAAAGCCCCA ATGTACTACA GATACGCCAG TTCATCCAGC ATGAGGTCAT AAACGATGAG 
GTGAAACTGA AGCTGCAGAT TTCCCTTAAG GACTATATAA ACCATATTTT AAAGAAAGAA 
GATGAGCTAC AGGAAATGAC AGTTAAAGAT TCCAGAACTG AGGAGGAGAG AGGCAATGCT 
GCAGATCTCC TCAAGCTTGT TATGTCTTTT CCTAAAATGG AGGAAACTAC AAAAGGTCAT 
GCTACTGAAG TTGCAGCCCA CTTAGTTGAT TTATTCAAGC AATCTTTGGC TGTTGCTCCA 
GAATGTCCAC CAGAAACATT TGATACAGAT TTCTTGGAAA AGAAAGGGAG GCACATAGCA 
AGTGAAAAGC GCTGGAAAGA AAAGATAGAT CAAAAGAGGA AGGAAATCGA AAAAACACAC 
AATTATCTAG AGGAAATCAG GAACAGAACG GTATCACCCT ACTTCAAATC AGAATTGAGC 
AAGACCTTTC AGTCTCAGTT TAATTATCTA GACGCCCTTT CCAAAAACTT GCCTCCTTTT 
ACAAAGAAAG TTGAAGATAC AAACAAAACA GCTGTTCAAA ATACATCCAA TCTTCAGCCA 
GATTCCCAGC CAAATAAAGT TTCACAACAA GAATCCTCAG ACACACTTAG TCTTCCAAAA 
TCAGAGGCTT TTGTGGATAT GAAGGCGTTC GCTAGCCAGA ACCAAACTGA AAATTTAACA 
AAATCTACTG AAGAACCAGA AACTTATGCC CCTGTGAAAA TTCCAACCCA GTCATTGCTG 
CAGGATGTTG CAGGACAAGC AAGGAAAGAG AAAGTGAGGT TACCTCGCTA TTTGCTGAGT 
TCCAAACCCA AGTCTCAACC TCTCGAGAAG GTGCAAGATT CTGTTGGAGG AAGAGTGAGA 
ACATCCTCGG TTGCTTCTGC TGCCATTAAT AACGCAAATT CATCCCCTTT TGGATTTCAT 
CTTCTCCCAC CGTCAGTGAA TTTTGGAGTG CTTGAAGAAG GGCACACCTA TGAAACCACT 
GTGAAGCTCA AGAATGTTGG AGTGGACTTC TGCAGGTTTA GGGTGAAGCA GCCGCCACCC 
AGCACAGGAT TGAAAGTGAC TTACAAACCT GGGCCTTTTC TTCTCGGTAT AGCTCAACAG 
CCTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023497100.1
CDS	67..6495
Translation	MAPKKEKSGSASANSKIWEPSLIAAHFNQNDWKGVIAFVVGNRIEDELLIGALTLAVQAPQRRLFSMVSWQDILQQINGTNALPGTASKKGKKPLGGSAPLYSEVLIATKAIMDSGEKLTLPLIGKLLKYQLLQIKYKDQQRRESEKKVTEDKFKTEKDKGKAKSPKEKKPSGTKVVKGKGKDQPEANTTVKKATQLKQRGEEDDAKRYIDDEPDDGAQHYIIVVGFNDPQLLAIMTELGIPISSVIKISSENYEPLQAHLAAVSQQQEVSLQPEDIEAEKLKNENTIKELEIFWKYLEPILNNEKPETNLFDVARLEYMVKEDGFPSDWSDSGMMLELGTDIFENIACLMYDSLDWKRQHRHYLESMQLINVPQVVNEKPILEAVAIAEAPQAAAPTPGKKKAQNEERQAPTSVPFVITTEADMRYYNDLLNPIPEEFISVPLILHCMLEQVVATEEDLVPPSLVEPPPRADGLDHRIAAHIVSILPSLCLTEKEKKNLQEIFLSEEENESKAVPKGPLLLNYHDTLAHKKYALKDPKNFDPVQIEQEMQSKLPLWEFLEFPLPPPWNSTKRLATIHELMHFCTNEVLSWNEVERAFKVFTFESLKLSTVDEKGKLKPARMMYGTDCEMFNIPWDNPARFAKQIRQQYITKMNTQEVKQKTDTETKDRILFVDQNMPVSVQDDESSKEPSDPSQADASNMQHSDSNNKKSLDPDDRELSEQDNSLKTQLQSEPLEQTTGNEIKDEAVTNVDPPEKKSKKMTVEAELEDIKKTEQRSLMDWSFTEHFKPKVLLQVLQEANQHYRCIDSYYHTQDNSLLLVFHNPMNLQRLHCEYWNVALHSNVGFRNYLELVAQSIQDWIIKEEAIYQESKMAEELSRSKLEQEVKSPASGKISSPSKIYSVKKSKSNKGMSKTELIDQEKEKEKEKEKIPFILEGSLKAWKEEQDRLAEEERLKEEKKAEKKSKDIGKKKGKEKVEREDSRVLKKKSFFKDKAKEEQIKISDVTEEPLQQPEPEIVYPFRGYNMGDIPIQISGTNCYLYPSDGGQIEVEKTTFEKGPTFIKVKVKKDKHNFIIHLKDPKKIVRNEKKEDDYSSEDEKDEGEEERNLEMKESDAENKAISKFGSFSATLENGICLSISYYGSSGMAPEDKDPNLEAMLNIPSALTPTIIPVVVTVPQGKGKGKTKGKEKPKDSIKEEEHPKEEEKKEEVEPEPVLQETVYVPTFQNLNVSCPSGLLVTFIGQESADHHVMDEEPTWDIMVRQSYPQKVKHYEFYKTVMPPVEQEASRVITSQGTVVKYMLDGSTQILFANGAVSSSPDSGPVCPPPEMPASPHNGDLMDSVSQQKSETAPSETVITKKGKGHKNQSSVPHTNEIHEPPQEVVQIVTPVEVHVGTWFTTTPDGNRIGTKGLEKIADLAPFLSFQATDPVNGTVMTTREDKVIIVEKKDGTRIVDHADGTRITTFYQVYEDHIMPPDDQEIPEHPQTLTRQVKCMRIESSHYATVITNCEDSSCCATFGDGTSIIAKPQGTYQVLPSNKGCLHIDRDCSAVYYHESSSDIYHPFQKREQLRASRYIMKHTSEVLCEVLDPEGNTFQVMADGSTSTLLPENNLEDDLNSKPEGYDSLSSTHLHKNHLQIYGEHVPRFFVIYADGSGVELLRDDDIEEYLSLAYGESTTVVLQEPVQEQPGALSITVLRPFHEASPWLMKKELDTIVPPNLQSRSWEKFPSVEKKTPGPPFGTHIWKGLNIECKQLVSAPAPILKSPNVLQIRQFIQHEVINDEVKLKLQISLKDYINHILKKEDELQEMTVKDSRTEEERGNAADLLKLVMSFPKMEETTKGHATEVAAHLVDLFKQSLAVAPECPPETFDTDFLEKKGRHIASEKRWKEKIDQKRKEIEKTHNYLEEIRNRTVSPYFKSELSKTFQSQFNYLDALSKNLPPFTKKVEDTNKTAVQNTSNLQPDSQPNKVSQQESSDTLSLPKSEAFVDMKAFASQNQTENLTKSTEEPETYAPVKIPTQSLLQDVAGQARKEKVRLPRYLLSSKPKSQPLEKVQDSVGGRVRTSSVASAAINNANSSPFGFHLLPPSVNFGVLEEGHTYETTVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPFLLGIAQQPP

Target ORF information:

RefSeq Version	XM_023641332.1
Organism	Equus caballus(horse)
Definition	Equus caballus sperm associated antigen 17 (SPAG17), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023641332.1

ORF Insert Sequence:

ATGGCACCCA AGAAAGAGAA AAGCGGGTCG GCGAGCGCGA ATTCTAAGAT ATGGGAACCC 
TCGCTCATCG CTGCACACTT CAATCAGAAC GATTGGAAGG GCGTCATCGC TTTTGTAGTT 
GGAAACCGGA TTGAGGATGA GCTTCTCATT GGAGCCCTCA CCCTCGCTGT CCAGGCCCCT 
CAGCGTAGAC TCTTCAGTAT GGTATCATGG CAAGACATCC TCCAGCAGAT CAATGGAACA 
AATGCGCTTC CTGGAACTGC ATCTAAAAAG GGAAAGAAAC CTCTAGGCGG TAGCGCTCCC 
TTATATTCTG AGGTGTTAAT AGCAACAAAG GCAATTATGG ACAGTGGAGA GAAATTAACC 
TTACCGCTGA TAGGGAAACT CTTGAAATAT CAGCTTCTCC AGATTAAATA TAAGGACCAA 
CAGCGACGGG AAAGCGAAAA GAAGGTGACA GAAGACAAAT TTAAGACTGA AAAGGATAAA 
GGGAAAGCGA AATCTCCCAA GGAAAAGAAG CCCTCAGGTA CCAAGGTTGT CAAAGGAAAG 
GGAAAGGATC AGCCTGAGGC AAACACAACA GTGAAAAAGG CCACTCAGTT AAAACAGAGG 
GGAGAAGAAG ACGACGCCAA ACGTTACATT GATGATGAGC CAGATGATGG TGCCCAACAT 
TACATTATAG TTGTGGGCTT CAACGATCCT CAGCTATTAG CGATTATGAC TGAGCTTGGC 
ATTCCCATAA GCAGCGTGAT TAAAATATCT TCAGAGAATT ATGAACCTCT GCAGGCGCAC 
CTGGCAGCAG TTAGCCAGCA GCAGGAAGTT TCTCTTCAGC CAGAAGATAT AGAAGCTGAA 
AAATTGAAGA ATGAGAATAC CATAAAAGAA CTTGAAATTT TCTGGAAGTA CTTGGAACCA 
ATCCTGAATA ATGAGAAACC TGAAACAAAT CTCTTTGATG TTGCTCGTCT TGAATACATG 
GTCAAGGAAG ATGGCTTTCC TTCTGACTGG TCAGACAGTG GGATGATGCT GGAATTGGGC 
ACCGACATTT TTGAAAATAT TGCCTGTTTG ATGTACGACA GCCTGGATTG GAAAAGGCAG 
CACCGGCACT ATTTAGAAAG CATGCAGCTT ATTAATGTCC CACAAGTGGT TAACGAGAAG 
CCTATCTTAG AAGCTGTAGC CATTGCTGAG GCTCCACAAG CTGCTGCTCC AACCCCTGGG 
AAGAAGAAAG CACAAAATGA AGAACGGCAA GCCCCAACAT CAGTGCCTTT TGTCATCACA 
ACTGAAGCAG ACATGAGATA TTACAATGAT TTGCTGAATC CGATTCCAGA AGAATTCATT 
TCTGTGCCCC TGATACTGCA CTGTATGCTG GAACAGGTTG TAGCAACTGA AGAAGACCTT 
GTCCCACCCA GTCTGGTGGA GCCGCCTCCC AGAGCAGATG GGTTGGACCA CAGAATTGCA 
GCTCACATTG TATCCATCCT ACCCTCTCTC TGTCTCACGG AGAAGGAGAA AAAGAATCTC 
CAGGAAATAT TTTTGTCTGA AGAAGAAAAT GAAAGCAAAG CAGTGCCCAA AGGTCCTCTC 
CTACTGAACT ATCACGACAC TCTGGCCCAC AAGAAGTATG CACTGAAGGA CCCAAAGAAT 
TTCGACCCAG TTCAAATTGA GCAGGAGATG CAGTCCAAGT TGCCACTGTG GGAATTCCTT 
GAATTCCCTC TGCCCCCGCC ATGGAACAGC ACTAAACGTC TAGCTACAAT TCATGAGCTC 
ATGCACTTTT GTACAAATGA AGTCTTGAGC TGGAATGAAG TAGAACGAGC CTTCAAAGTG 
TTTACTTTTG AGAGCCTGAA ACTCTCTACA GTTGATGAAA AAGGGAAACT AAAGCCTGCT 
CGGATGATGT ATGGGACTGA TTGTGAGATG TTCAACATAC CATGGGACAA CCCTGCCAGA 
TTTGCTAAAC AGATAAGGCA ACAATACATC ACGAAAATGA ACACCCAAGA GGTCAAACAG 
AAAACAGATA CTGAAACCAA AGACAGAATT TTGTTTGTGG ATCAGAATAT GCCAGTGTCT 
GTGCAAGATG ATGAGAGCAG TAAAGAACCT TCAGACCCTA GTCAGGCTGA TGCTAGCAAT 
ATGCAGCATT CTGACTCGAA TAATAAGAAA TCCTTAGACC CTGATGATAG AGAGCTGTCA 
GAGCAGGACA ACAGCTTGAA GACTCAGCTC CAATCTGAGC CTTTGGAGCA GACCACGGGC 
AATGAGATCA AAGATGAAGC AGTCACAAAT GTTGATCCTC CTGAAAAGAA ATCCAAGAAG 
ATGACCGTGG AAGCAGAGTT GGAGGACATA AAGAAAACAG AGCAACGCAG CCTAATGGAT 
TGGAGTTTCA CTGAACATTT TAAACCGAAA GTTCTACTGC AGGTTCTTCA AGAAGCCAAT 
CAGCATTACA GATGTATTGA TTCTTATTAC CACACCCAAG ACAACTCTTT ACTATTAGTC 
TTTCATAATC CAATGAATTT ACAACGTCTG CATTGTGAAT ATTGGAATGT TGCTCTTCAC 
TCCAATGTTG GATTCAGGAA TTATTTGGAA CTTGTTGCAC AGTCCATTCA AGACTGGATC 
ATAAAAGAAG AAGCTATATA TCAGGAATCT AAAATGGCTG AGGAACTCAG CAGGAGCAAG 
CTTGAGCAGG AAGTGAAATC TCCTGCTAGT GGCAAAATTT CTTCCCCTAG CAAAATTTAT 
TCTGTTAAAA AATCTAAAAG TAACAAAGGA ATGAGCAAAA CAGAGTTAAT AGATCAAGAA 
AAAGAAAAAG AGAAAGAGAA GGAAAAGATT CCTTTCATTT TAGAAGGCTC TCTCAAGGCA 
TGGAAAGAAG AGCAAGATCG ATTAGCAGAA GAGGAACGCT TAAAGGAAGA AAAAAAAGCA 
GAGAAGAAGA GTAAGGACAT TGGTAAAAAG AAAGGCAAGG AAAAGGTAGA GAGAGAAGAT 
AGCAGGGTTT TGAAGAAAAA ATCTTTTTTC AAGGATAAAG CTAAAGAAGA GCAAATCAAG 
ATCTCAGACG TAACAGAGGA GCCCCTTCAA CAGCCAGAAC CTGAGATAGT TTACCCGTTT 
CGCGGATACA ATATGGGAGA TATACCCATC CAAATCTCGG GAACAAATTG TTATCTGTAT 
CCTTCAGATG GAGGGCAGAT TGAAGTAGAA AAGACAACAT TTGAAAAAGG TCCAACTTTT 
ATCAAAGTGA AAGTGAAAAA GGACAAGCAC AATTTTATAA TTCATTTAAA AGATCCTAAG 
AAAATTGTGA GAAACGAAAA AAAAGAAGAC GATTATTCTT CAGAAGATGA GAAAGATGAA 
GGAGAGGAAG AACGAAATCT TGAAATGAAA GAATCAGATG CAGAGAATAA AGCTATCAGC 
AAGTTTGGAT CTTTTTCTGC CACCTTGGAA AATGGAATCT GCCTCTCAAT AAGTTACTAC 
GGATCAAGTG GAATGGCGCC AGAGGATAAG GATCCTAATT TGGAAGCAAT GTTGAATATC 
CCGTCAGCAC TCACTCCAAC AATAATTCCT GTGGTAGTGA CTGTTCCTCA AGGCAAAGGA 
AAAGGGAAAA CCAAAGGCAA AGAAAAGCCC AAAGACTCTA TTAAAGAAGA GGAACATCCA 
AAAGAAGAAG AGAAAAAGGA AGAAGTAGAA CCAGAACCTG TTTTACAAGA GACTGTTTAT 
GTTCCCACCT TCCAAAACCT AAATGTGTCT TGCCCCAGTG GGCTTCTAGT GACCTTCATT 
GGCCAAGAGT CTGCAGATCA CCATGTTATG GATGAGGAAC CCACTTGGGA CATCATGGTC 
CGACAGAGCT ACCCCCAGAA GGTGAAGCAC TATGAGTTCT ACAAAACGGT GATGCCACCA 
GTGGAGCAGG AGGCATCGAG AGTTATCACT AGTCAAGGCA CTGTTGTCAA ATACATGTTG 
GATGGATCCA CACAGATTCT CTTTGCAAAT GGCGCTGTGA GCAGTAGTCC CGATTCAGGT 
CCTGTTTGTC CTCCTCCTGA AATGCCAGCG AGCCCTCATA ACGGAGACTT GATGGACAGT 
GTTTCTCAGC AAAAATCAGA AACAGCACCG TCTGAGACTG TCATCACAAA GAAAGGAAAA 
GGTCACAAAA ATCAGTCATC AGTGCCACAT ACGAATGAAA TCCATGAACC TCCCCAAGAG 
GTAGTTCAAA TAGTAACACC TGTGGAGGTT CATGTAGGCA CCTGGTTTAC AACTACACCT 
GACGGAAATC GGATTGGCAC CAAAGGATTA GAAAAAATAG CAGACTTGGC CCCATTCTTA 
TCCTTTCAGG CCACAGATCC TGTCAATGGA ACGGTTATGA CAACTCGAGA GGACAAGGTG 
ATCATTGTTG AAAAGAAGGA TGGTACTCGG ATCGTGGATC ATGCCGATGG TACCAGAATC 
ACAACCTTTT ATCAAGTTTA TGAAGATCAT ATTATGCCTC CAGATGATCA AGAAATACCC 
GAACATCCTC AAACTCTCAC CAGGCAGGTG AAGTGCATGC GCATAGAAAG CTCACACTAT 
GCCACTGTCA TCACCAACTG CGAGGACAGT AGCTGCTGTG CCACTTTTGG AGATGGGACG 
TCTATTATTG CAAAGCCACA GGGAACATAC CAGGTGTTAC CTTCAAACAA AGGCTGTCTT 
CATATTGATA GAGACTGTTC AGCTGTATAT TACCATGAAT CCAGCAGTGA TATATACCAT 
CCTTTCCAAA AACGTGAGCA GCTGAGAGCT AGCAGGTACA TCATGAAGCA CACGTCAGAG 
GTTCTCTGTG AAGTCCTGGA TCCTGAGGGA AACACCTTTC AGGTCATGGC TGATGGTAGC 
ACATCAACTC TGCTACCTGA AAACAATTTG GAAGATGATT TAAACTCAAA ACCTGAAGGC 
TATGATAGTT TATCATCTAC TCACCTTCAT AAAAATCACC TACAAATATA TGGTGAACAT 
GTCCCCAGGT TTTTTGTTAT CTATGCCGAT GGATCAGGAG TAGAACTTCT TCGAGATGAT 
GACATAGAAG AATACTTATC CTTGGCATAT GGAGAATCAA CCACTGTAGT TCTTCAAGAG 
CCAGTACAAG AACAGCCAGG CGCCCTGAGC ATCACTGTTC TTCGCCCTTT TCATGAAGCA 
TCGCCATGGC TAATGAAGAA AGAATTAGAC ACAATTGTTC CTCCTAATCT CCAGTCAAGG 
TCATGGGAGA AATTTCCCTC AGTTGAGAAA AAAACTCCAG GACCTCCCTT TGGCACTCAT 
ATTTGGAAGG GCCTTAACAT TGAGTGCAAA CAGCTAGTGA GTGCCCCAGC TCCTATACTC 
AAAAGCCCCA ATGTACTACA GATACGCCAG TTCATCCAGC ATGAGGTCAT AAACGATGAG 
GTGAAACTGA AGCTGCAGAT TTCCCTTAAG GACTATATAA ACCATATTTT AAAGAAAGAA 
GATGAGCTAC AGGAAATGAC AGTTAAAGAT TCCAGAACTG AGGAGGAGAG AGGCAATGCT 
GCAGATCTCC TCAAGCTTGT TATGTCTTTT CCTAAAATGG AGGAAACTAC AAAAGGTCAT 
GCTACTGAAG TTGCAGCCCA CTTAGTTGAT TTATTCAAGC AATCTTTGGC TGTTGCTCCA 
GAATGTCCAC CAGAAACATT TGATACAGAT TTCTTGGAAA AGAAAGGGAG GCACATAGCA 
AGTGAAAAGC GCTGGAAAGA AAAGATAGAT CAAAAGAGGA AGGAAATCGA AAAAACACAC 
AATTATCTAG AGGAAATCAG GAACAGAACG GTATCACCCT ACTTCAAATC AGAATTGAGC 
AAGACCTTTC AGTCTCAGTT TAATTATCTA GACGCCCTTT CCAAAAACTT GCCTCCTTTT 
ACAAAGAAAG TTGAAGATAC AAACAAAACA GCTGTTCAAA ATACATCCAA TCTTCAGCCA 
GATTCCCAGC CAAATAAAGT TTCACAACAA GAATCCTCAG ACACACTTAG TCTTCCAAAA 
TCAGAGGCTT TTGTGGATAT GAAGGCGTTC GCTAGCCAGA ACCAAACTGA AAATTTAACA 
AAATCTACTG AAGAACCAGA AACTTATGCC CCTGTGAAAA TTCCAACCCA GTCATTGCTG 
CAGGATGTTG CAGGACAAGC AAGGAAAGAG AAAGTGAGGT TACCTCGCTA TTTGCTGAGT 
TCCAAACCCA AGTCTCAACC TCTCGAGAAG GTGCAAGATT CTGTTGGAGG AAGAGTGAGA 
ACATCCTCGG TTGCTTCTGC TGCCATTAAT AACGCAAATT CATCCCCTTT TGGATTTCAT 
CTTCTCCCAC CGTCAGTGAA TTTTGGAGTG CTTGAAGAAG GGCACACCTA TGAAACCACT 
GTGAAGCTCA AGAATGTTGG AGTGGACTTC TGCAGGTTTA GGGTGAAGCA GCCGCCACCC 
AGCACAGGAT TGAAAGTGAC TTACAAACCT GGGCCTTTTC TTCTCGGTAT AGCTCAACAG 
CCTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SPAG17 cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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