SHROOM4 cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	SHROOM4
Entrez Gene ID	100052405
Full Name	shroom family member 4
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014728850.1	XP_014584336.1	protein Shroom4
XM_023633893.1	XP_023489661.1	protein Shroom4 isoform X1
XM_014728850.2	XP_014584336.2	protein Shroom4 isoform X2

Pathways from BioSystems
Gene Ontology

The following SHROOM4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHROOM4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHb38997	XM_014728850.1 Latest version!	Equus caballus shroom family member 4 (SHROOM4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHb71868	XM_014728850.2 Latest version!	Equus caballus shroom family member 4 (SHROOM4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHb71867	XM_023633893.1 Latest version!	Equus caballus shroom family member 4 (SHROOM4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SHROOM4 ( XM_014728850.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014584336.1 Equus caballus shroom family member 4 (SHROOM4), mRNA.

Price & Availability↑

CloneID	OHb38997
Clone ID Related Accession (Same CDS sequence)	XM_014728850.1
Accession Version	XM_014728850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1447862400000
Organism	Equus caballus(horse)
Product	protein Shroom4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001877040.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTTG GCCATTTGGA GACCTCAAGG CAGGGCTCAC AAAGCCTTCC AGCAGAGCAA 
GAGTCTTTTG TTCACCATTC CAGTGATTTC CTGCCTTCAA TAAGGGGCCA CTTGGGATCT 
CAACCTGAGA AGGCTCAGCC CCCTTGCTAT TATGGCATTG GTGGGCTTTG GAGGACATCA 
GAGCAGGAGA CCACTGATTC CCCCAAACCA GAAGCCTTGA TGACAGAAGA GTCCAAACCC 
AAGCCAGCAT GGAGCCAGAG CTACTTCTTT CCTGAGGAGC AGTTCTCTTT TATGGGCTGG 
GGCTCTGAGA AGCAACACCT CAGCCCCGCG GGCTTCGGTG AACCCAAGAC AACAGGACAA 
GAGTTTCAGC ACTTCTCGCC TCCTCAGGGG GCCCCAGGGA TCCCTACCTC TTACTCAGCT 
TATTACAATA TTTCTGTGGC CAAGGCAGAG CTGCTGAACA AGTTGAAAGA CCAGCCCGAG 
ATGGTAGAGA TTGGTCTGGG AGAAGAGGAA GTTGACCATG AACTGGCTCA AAAAAAGATA 
CAGCTTATTG AAAGCATTGG CAGAAAACTT TCTGTCCTGC GGGAGGCCCA GCGAGGACTG 
CTGGAGGACA TCAGTGCCAA TTCTGCCCTT GGGGAGGAGG TGGAAGCCAA CCTGAAAGCC 
GTCTGCAAAT CCAATGAGTT TGAAAAATAT CATTTGTTTA TCGGGGACCT AGATAAAGTG 
GTCAACCTGT TGCTGTCACT GTCTGGACGA CTGGCCCGGG TGGAGAATGC TCTCAACAGC 
ATCGATTCAG AGGCCAACCA GGAGAAGTTG GTGCTGATAG AGAAAAAGCA GCAGTTGACA 
GGGCAGTTGG CAGATGCCAA GGAGCTGAAG GAGCACGTGG ACCGTCGGGA GAAGTTGGTG 
TTTGGTATGG TCTCCCGCTA CCTGCCTCAA GACCAGCTCC AAGATTACCA GCACTTTGTA 
AAGATGAAAT CTGCCCTCAT CATTGAACAG CGAGAGCTGG AAGAGAAGAT CAAGCTCGGG 
GAAGAGCAGC TCAAATGTCT CAGGGAGAGC CTACTCCTGG GGCCCAGCAA TTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014584336.1
CDS	140..1216
Translation	MAFGHLETSRQGSQSLPAEQESFVHHSSDFLPSIRGHLGSQPEKAQPPCYYGIGGLWRTSEQETTDSPKPEALMTEESKPKPAWSQSYFFPEEQFSFMGWGSEKQHLSPAGFGEPKTTGQEFQHFSPPQGAPGIPTSYSAYYNISVAKAELLNKLKDQPEMVEIGLGEEEVDHELAQKKIQLIESIGRKLSVLREAQRGLLEDISANSALGEEVEANLKAVCKSNEFEKYHLFIGDLDKVVNLLLSLSGRLARVENALNSIDSEANQEKLVLIEKKQQLTGQLADAKELKEHVDRREKLVFGMVSRYLPQDQLQDYQHFVKMKSALIIEQRELEEKIKLGEEQLKCLRESLLLGPSNF

Target ORF information:

RefSeq Version	XM_014728850.1
Organism	Equus caballus(horse)
Definition	Equus caballus shroom family member 4 (SHROOM4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014728850.1

ORF Insert Sequence:

ATGGCCTTTG GCCATTTGGA GACCTCAAGG CAGGGCTCAC AAAGCCTTCC AGCAGAGCAA 
GAGTCTTTTG TTCACCATTC CAGTGATTTC CTGCCTTCAA TAAGGGGCCA CTTGGGATCT 
CAACCTGAGA AGGCTCAGCC CCCTTGCTAT TATGGCATTG GTGGGCTTTG GAGGACATCA 
GAGCAGGAGA CCACTGATTC CCCCAAACCA GAAGCCTTGA TGACAGAAGA GTCCAAACCC 
AAGCCAGCAT GGAGCCAGAG CTACTTCTTT CCTGAGGAGC AGTTCTCTTT TATGGGCTGG 
GGCTCTGAGA AGCAACACCT CAGCCCCGCG GGCTTCGGTG AACCCAAGAC AACAGGACAA 
GAGTTTCAGC ACTTCTCGCC TCCTCAGGGG GCCCCAGGGA TCCCTACCTC TTACTCAGCT 
TATTACAATA TTTCTGTGGC CAAGGCAGAG CTGCTGAACA AGTTGAAAGA CCAGCCCGAG 
ATGGTAGAGA TTGGTCTGGG AGAAGAGGAA GTTGACCATG AACTGGCTCA AAAAAAGATA 
CAGCTTATTG AAAGCATTGG CAGAAAACTT TCTGTCCTGC GGGAGGCCCA GCGAGGACTG 
CTGGAGGACA TCAGTGCCAA TTCTGCCCTT GGGGAGGAGG TGGAAGCCAA CCTGAAAGCC 
GTCTGCAAAT CCAATGAGTT TGAAAAATAT CATTTGTTTA TCGGGGACCT AGATAAAGTG 
GTCAACCTGT TGCTGTCACT GTCTGGACGA CTGGCCCGGG TGGAGAATGC TCTCAACAGC 
ATCGATTCAG AGGCCAACCA GGAGAAGTTG GTGCTGATAG AGAAAAAGCA GCAGTTGACA 
GGGCAGTTGG CAGATGCCAA GGAGCTGAAG GAGCACGTGG ACCGTCGGGA GAAGTTGGTG 
TTTGGTATGG TCTCCCGCTA CCTGCCTCAA GACCAGCTCC AAGATTACCA GCACTTTGTA 
AAGATGAAAT CTGCCCTCAT CATTGAACAG CGAGAGCTGG AAGAGAAGAT CAAGCTCGGG 
GAAGAGCAGC TCAAATGTCT CAGGGAGAGC CTACTCCTGG GGCCCAGCAA TTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHROOM4 ( XM_014728850.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_014584336.2 Equus caballus shroom family member 4 (SHROOM4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHb71868
Clone ID Related Accession (Same CDS sequence)	XM_014728850.2
Accession Version	XM_014728850.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	protein Shroom4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009175.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014728850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTCC CTTCTGAAGC CTTCAGCTTG TCCTGGCATT CCGGCTGCAA CACAAGTGAC 
GTGTGTGTGC AGTGGTGTCC ACTCTCCCGG CACTGTAGCA CTGAGAAAAG CAGCTCCATT 
GGCAGCATGG AGAGCCTGGA GCAACCAAGC CAAGCCACCT ATGAGGGCCA CCTCTTGCCC 
ATTGACCAGA ACATGTACCC CAACCAGCGT GACTCAGCCT ACAGCTCCTT CTCGGCCAGC 
TCAAATGCCT CTGACTGTGC CCTTTCCCTC AGGCCAGAGG AGCCAGCCTC TGCTGACTGC 
ATCGTGCAAG GCCCAGGGCC AACTAAGGCC CCTAATGGCC GGCCTAATGT GGCTGACACC 
TCAGGAGGTA GCCAGCGCAC CAATGGGGGC CACCTGACTT CCAGCTCCCA GATGTCATCC 
TGCCCACAGG AGGTCCACCA CTCAGGGCCT GCTAAGACTA CCAGGGGCCC ACCTCAACCT 
CCAGTGAGGC GAGACAGTCT TCAGGCCTCC AGAGCCCAGC TCCTCAATGG AGAGCAGCGC 
AGGGCTTCTG AACCTGTGAA CTCCTTGCAA CAGAAGGAGA AACCAAGCTT AGAAACCATT 
CTATTCCCAA GAAACCCTAA CAGGTTCTGC TGCCTCAGTG GGCAAGATCA AGTGACAAAT 
GAGGGCCATC AGAACTGTGA ACTCAGCCAA ACTCCTGAAC CCAGCCAGCA GGGCTCTGAG 
CATCTACTGA TGGAGGCCTC AGCCAAAGCT GTTGGATTCC CAAAAGCATA TGACAAAGCT 
TCCAGCGTAG ATTCCAGCCC ACTCAACAAG GCTTCAGCAG AGCTAGCTAA GGCTTCTTCT 
TTTGGCAGCA CTCTACACCT CACAGGGCAT CGCCATAGTG CGCCTGAACA GCTGCTGGCA 
TCCCACTTAC AGCGTGTGCA CCTTGATACC AGGGGCAGCA AGGGAATAGA GCTCCCAACT 
GGGCAGGATG GGCATGAGTG GACTCTGTCT CCTTTGCATA GCAGCCACAC AGAGAAGAAA 
AGTCCATGTC CGTCTGCAGG AGGAACCCAG GACCAGCCCA GCAAAGAGAG AAAGACTAGG 
CCAGTGGATG ATAGGCCTTT AGGTTCAGGC CACCAGAGCC CAAGCAGTTC TCCACATGGA 
GAGGCTGATG GACACCCTCC AGAAAGAGGT TTCCAGGACC CAAACAGAGT AGGCAGTGAA 
TTGGCCATCC AGCAGCCCTC TGCTTCTGAC TCGCTTATTC AACAAACCAG GGACTGTTCT 
GCAACCACTA AAGTACCTGG CAGCACAGAG GCAACTGAAG AAGGGGACAA GGAGCCCAAG 
GAGTGTGGCC GGGTGGGTGG TAGGCGAAAT GGAGGGACCC GGGGCCGCTC AATCCAAAAC 
CGGCGGAAGA GTGAGCGTTT TGCTACCAAT CTGCGTAATG AAATTCAGAG GAGGAAGGCT 
CAGCTCCAGA AAAGCAAGAA TCCCTTGTCA CAGCTGTGTG ACACTAAGGA GCCAGTGGAA 
GAGACCGAGG AGCCCCTAGA AAGTCCTTCA CTTCCCCCCT CTAACTCATC TCTTCTATCT 
TCATATAAAA AACCACCTAG CCCCAGAGAC AAGCTCTTCA ACAAGAGCAT GATTCTCAGG 
GCTAGGTCTT CAGAGTGCCT CAGCCAAGCC CCTGAGAGCC ATGAATCTAG GACAGGCTTG 
GAGGGACAAA TAAGCCCTGG CCAGAGGCCC GGCCAGGCCT CTTTGGGCCT GAATACCTGG 
TGGAAAGCAT CTGACCCATC CTCCTCAGAC TCTGAGAAAA CAGATGTTCA CTGTGGAGTC 
CGTGGAGGTC AATGGAGATG GTCTCCAGAG CACAACCTGC AGCCACATGT GGCACTAGCC 
ATGGAAGGCC CTTCCAACCC AGGTGACAAC AAAGAATTGA AGGCTTCTAC TTCCCAAGCT 
GGAGAGGAAA CCATCCTCTT GCCCTTTGCA GACAGAAGAA AATTCTTTGA AGAGAGTAGC 
AAATCCGTAT CTACATCTCA TTTGCCAGGT TTAACCACAC ATGGCAACAA GACTTTTACC 
CAGAGACCAA AACCTATAGA CCGAAACTTC CAGCCAATGA GCTCCACCTA TAGGGAATTG 
AGGCGCCATC CCATGGACCA ACCATATCAT GCCACAGACC AATCATATCA TTCCATGTCA 
CCCCTTCAGT CAGAAACTCC CACTTACTCC GAATGTTTTG TGAGCAAGGG TCTAGAACAA 
TCCATGTGTT GTAAGCCACT GCACTGTGGT GATTTCGATT ACCACAGGAC CTGCTCTTAT 
TCCTGCAATG TCCAGGGAGC TGTAGTCCAT GACCCTTGCA TTTATTGTTC TGGGGAAATC 
TGCCCTGCTC TGCGAAAAAG AAATATGATG CCCAACTGCT ACAACTGCCG GTGCCATCAC 
CACCAGTGCA TCCGGTGTTC AGCTTGCCAT CATAATCCTC AGCACAGTAC CCTCGAGGAC 
AGCAGCTTGG CACCTGGCAA CACTTGGAAA CCCAGGAAGC CGACAGTGCA GGAATTTCCT 
GGGGACAAAT GGAAACCAAT AACAGGAAAC AGGAAGACCA GCCAGTCAGG GAGGGAAATG 
GCTCATTCCA AGGCTAGCTT TTCATGGGCA ACCCCCTTCC ATCCTTGCAC TGAGAACCCA 
GCACTCGACT TGTCAAGCTA CCGAGCAATC TCTTCTCTTG ACCTCCTGGG AGACTTCAAA 
CACTCCTTGA AAAAAACAGA GGAAACTTCA GTATATGAGG AGGGGAGCTC CATGGCCTCC 
GTGCCCCGGC CACTACGCAG CCGTGCCTTC TCAGAGAGCC ATATCAGCTT GGAGCCCCAG 
AGCTCCCGGG CCTGGGGGCA GCATAGGAGG GAGATCTTTA CCAAAGTTGA TGAGACCCAG 
CCGGATCCTC TGGGAGCCAG GAAGAAGGCC TTTCCTCCTC CTCGCCCTCC TCCTCCCAAC 
TGGGAGAAGT ACAGGCTTTT CCGTGCAGCC CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAAC AGCAGCAACA GCAGCAACAG CAACAGCAGC AACAGCAACA GCAACAGCAG 
CAACAGCAAC AGCAGCAGCA AGAGGAGGAG GAAGAAGAGG AGGAGGAAGA GGAGGAAGAA 
GAGGAAGAGG GAGTAGAAGA GGAGGAAGAG GAGGGGGAGG AGGAGGAGCT GCCACCCCAG 
TATTTCAGTT CAGAAACTAC TAGTTCCTGT ACTCTCAATC CTGAGGAGGT CCTGGTGCAG 
GCACAACCCA TGGCCTTTGG CCATTTGGAG ACCTCAAGGC AGGGCTCACA AAGCCTTCCA 
GCAGAGCAAG AGTCTTTTGT TCACCATTCC AATGATTTCC TGCCTTCAAT AAGGGGCCAC 
TTGGGATCTC AACCTGAGAA GGCTCAGCCC CCTTGCTATT ATGGCATTGG TGGGCTTTGG 
AGGACATCAG AGCAGGAGAC CACTGATTCC CCCAAACCAG AAGCCTTGAT GACAGAAGAG 
TCCAAACCCA AGCCAGCATG GAGCCAGAGC TACTTCTTTC CTGAGGAGCA GTTCTCTTTT 
ATGGGCTGGG GCTCTGAGAA GCAACACCTC AGCCCCGCGG GCTTCGGTGA ACCCAAGACA 
ACAGGACAAG AGTTTCAGCA CTTCTCGCCT CCTCAGGGGG CCCCAGGGAT CCCTACCTCT 
TACTCAGCTT ATTACAATAT TTCTGTGGCC AAGGCAGAGC TGCTGAACAA GTTGAAAGAC 
CAGCCCGAGA TGGTAGAGAT TGGTCTGGGA GAAGAGGAAG TTGACCATGA ACTGGCTCAA 
AAAAAGATAC AGCTTATTGA AAGCATTGGC AGAAAACTTT CTGTCCTGCG GGAGGCCCAG 
CGAGGACTGC TGGAGGACAT CAGTGCCAAT TCTGCCCTTG GGGAGGAGGT GGAAGCCAAC 
CTGAAAGCCG TCTGCAAATC CAATGAGTTT GAAAAATATC ATTTGTTTAT CGGGGACCTA 
GATAAAGTGG TCAACCTGTT GCTGTCACTG TCTGGACGAC TGGCCCGGGT GGAGAATGCT 
CTCAACAGCA TCGATTCAGA GGCCAACCAG GAGAAGTTGG TGCTGATAGA GAAAAAGCAG 
CAGTTGACAG GGCAGTTGGC AGATGCCAAG GAGCTGAAGG AGCACGTGGA CCGTCGGGAG 
AAGTTGGTGT TTGGTATGGT CTCCCGCTAC CTGCCTCAAG ACCAGCTCCA AGATTACCAG 
CACTTTGTAA AGATGAAATC TGCCCTCATC ATTGAACAGC GAGAGCTGGA AGAGAAGATC 
AAGCTCGGGG AAGAGCAGCT CAAATGTCTC AGGGAGAGCC TACTCCTGGG GCCCAGCAAT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014584336.2
CDS	506..4831
Translation	MHFPSEAFSLSWHSGCNTSDVCVQWCPLSRHCSTEKSSSIGSMESLEQPSQATYEGHLLPIDQNMYPNQRDSAYSSFSASSNASDCALSLRPEEPASADCIVQGPGPTKAPNGRPNVADTSGGSQRTNGGHLTSSSQMSSCPQEVHHSGPAKTTRGPPQPPVRRDSLQASRAQLLNGEQRRASEPVNSLQQKEKPSLETILFPRNPNRFCCLSGQDQVTNEGHQNCELSQTPEPSQQGSEHLLMEASAKAVGFPKAYDKASSVDSSPLNKASAELAKASSFGSTLHLTGHRHSAPEQLLASHLQRVHLDTRGSKGIELPTGQDGHEWTLSPLHSSHTEKKSPCPSAGGTQDQPSKERKTRPVDDRPLGSGHQSPSSSPHGEADGHPPERGFQDPNRVGSELAIQQPSASDSLIQQTRDCSATTKVPGSTEATEEGDKEPKECGRVGGRRNGGTRGRSIQNRRKSERFATNLRNEIQRRKAQLQKSKNPLSQLCDTKEPVEETEEPLESPSLPPSNSSLLSSYKKPPSPRDKLFNKSMILRARSSECLSQAPESHESRTGLEGQISPGQRPGQASLGLNTWWKASDPSSSDSEKTDVHCGVRGGQWRWSPEHNLQPHVALAMEGPSNPGDNKELKASTSQAGEETILLPFADRRKFFEESSKSVSTSHLPGLTTHGNKTFTQRPKPIDRNFQPMSSTYRELRRHPMDQPYHATDQSYHSMSPLQSETPTYSECFVSKGLEQSMCCKPLHCGDFDYHRTCSYSCNVQGAVVHDPCIYCSGEICPALRKRNMMPNCYNCRCHHHQCIRCSACHHNPQHSTLEDSSLAPGNTWKPRKPTVQEFPGDKWKPITGNRKTSQSGREMAHSKASFSWATPFHPCTENPALDLSSYRAISSLDLLGDFKHSLKKTEETSVYEEGSSMASVPRPLRSRAFSESHISLEPQSSRAWGQHRREIFTKVDETQPDPLGARKKAFPPPRPPPPNWEKYRLFRAAQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQEEEEEEEEEEEEEEEEGVEEEEEEGEEEELPPQYFSSETTSSCTLNPEEVLVQAQPMAFGHLETSRQGSQSLPAEQESFVHHSNDFLPSIRGHLGSQPEKAQPPCYYGIGGLWRTSEQETTDSPKPEALMTEESKPKPAWSQSYFFPEEQFSFMGWGSEKQHLSPAGFGEPKTTGQEFQHFSPPQGAPGIPTSYSAYYNISVAKAELLNKLKDQPEMVEIGLGEEEVDHELAQKKIQLIESIGRKLSVLREAQRGLLEDISANSALGEEVEANLKAVCKSNEFEKYHLFIGDLDKVVNLLLSLSGRLARVENALNSIDSEANQEKLVLIEKKQQLTGQLADAKELKEHVDRREKLVFGMVSRYLPQDQLQDYQHFVKMKSALIIEQRELEEKIKLGEEQLKCLRESLLLGPSNF

Target ORF information:

RefSeq Version	XM_014728850.2
Organism	Equus caballus(horse)
Definition	Equus caballus shroom family member 4 (SHROOM4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014728850.2

ORF Insert Sequence:

ATGCATTTCC CTTCTGAAGC CTTCAGCTTG TCCTGGCATT CCGGCTGCAA CACAAGTGAC 
GTGTGTGTGC AGTGGTGTCC ACTCTCCCGG CACTGTAGCA CTGAGAAAAG CAGCTCCATT 
GGCAGCATGG AGAGCCTGGA GCAACCAAGC CAAGCCACCT ATGAGGGCCA CCTCTTGCCC 
ATTGACCAGA ACATGTACCC CAACCAGCGT GACTCAGCCT ACAGCTCCTT CTCGGCCAGC 
TCAAATGCCT CTGACTGTGC CCTTTCCCTC AGGCCAGAGG AGCCAGCCTC TGCTGACTGC 
ATCGTGCAAG GCCCAGGGCC AACTAAGGCC CCTAATGGCC GGCCTAATGT GGCTGACACC 
TCAGGAGGTA GCCAGCGCAC CAATGGGGGC CACCTGACTT CCAGCTCCCA GATGTCATCC 
TGCCCACAGG AGGTCCACCA CTCAGGGCCT GCTAAGACTA CCAGGGGCCC ACCTCAACCT 
CCAGTGAGGC GAGACAGTCT TCAGGCCTCC AGAGCCCAGC TCCTCAATGG AGAGCAGCGC 
AGGGCTTCTG AACCTGTGAA CTCCTTGCAA CAGAAGGAGA AACCAAGCTT AGAAACCATT 
CTATTCCCAA GAAACCCTAA CAGGTTCTGC TGCCTCAGTG GGCAAGATCA AGTGACAAAT 
GAGGGCCATC AGAACTGTGA ACTCAGCCAA ACTCCTGAAC CCAGCCAGCA GGGCTCTGAG 
CATCTACTGA TGGAGGCCTC AGCCAAAGCT GTTGGATTCC CAAAAGCATA TGACAAAGCT 
TCCAGCGTAG ATTCCAGCCC ACTCAACAAG GCTTCAGCAG AGCTAGCTAA GGCTTCTTCT 
TTTGGCAGCA CTCTACACCT CACAGGGCAT CGCCATAGTG CGCCTGAACA GCTGCTGGCA 
TCCCACTTAC AGCGTGTGCA CCTTGATACC AGGGGCAGCA AGGGAATAGA GCTCCCAACT 
GGGCAGGATG GGCATGAGTG GACTCTGTCT CCTTTGCATA GCAGCCACAC AGAGAAGAAA 
AGTCCATGTC CGTCTGCAGG AGGAACCCAG GACCAGCCCA GCAAAGAGAG AAAGACTAGG 
CCAGTGGATG ATAGGCCTTT AGGTTCAGGC CACCAGAGCC CAAGCAGTTC TCCACATGGA 
GAGGCTGATG GACACCCTCC AGAAAGAGGT TTCCAGGACC CAAACAGAGT AGGCAGTGAA 
TTGGCCATCC AGCAGCCCTC TGCTTCTGAC TCGCTTATTC AACAAACCAG GGACTGTTCT 
GCAACCACTA AAGTACCTGG CAGCACAGAG GCAACTGAAG AAGGGGACAA GGAGCCCAAG 
GAGTGTGGCC GGGTGGGTGG TAGGCGAAAT GGAGGGACCC GGGGCCGCTC AATCCAAAAC 
CGGCGGAAGA GTGAGCGTTT TGCTACCAAT CTGCGTAATG AAATTCAGAG GAGGAAGGCT 
CAGCTCCAGA AAAGCAAGAA TCCCTTGTCA CAGCTGTGTG ACACTAAGGA GCCAGTGGAA 
GAGACCGAGG AGCCCCTAGA AAGTCCTTCA CTTCCCCCCT CTAACTCATC TCTTCTATCT 
TCATATAAAA AACCACCTAG CCCCAGAGAC AAGCTCTTCA ACAAGAGCAT GATTCTCAGG 
GCTAGGTCTT CAGAGTGCCT CAGCCAAGCC CCTGAGAGCC ATGAATCTAG GACAGGCTTG 
GAGGGACAAA TAAGCCCTGG CCAGAGGCCC GGCCAGGCCT CTTTGGGCCT GAATACCTGG 
TGGAAAGCAT CTGACCCATC CTCCTCAGAC TCTGAGAAAA CAGATGTTCA CTGTGGAGTC 
CGTGGAGGTC AATGGAGATG GTCTCCAGAG CACAACCTGC AGCCACATGT GGCACTAGCC 
ATGGAAGGCC CTTCCAACCC AGGTGACAAC AAAGAATTGA AGGCTTCTAC TTCCCAAGCT 
GGAGAGGAAA CCATCCTCTT GCCCTTTGCA GACAGAAGAA AATTCTTTGA AGAGAGTAGC 
AAATCCGTAT CTACATCTCA TTTGCCAGGT TTAACCACAC ATGGCAACAA GACTTTTACC 
CAGAGACCAA AACCTATAGA CCGAAACTTC CAGCCAATGA GCTCCACCTA TAGGGAATTG 
AGGCGCCATC CCATGGACCA ACCATATCAT GCCACAGACC AATCATATCA TTCCATGTCA 
CCCCTTCAGT CAGAAACTCC CACTTACTCC GAATGTTTTG TGAGCAAGGG TCTAGAACAA 
TCCATGTGTT GTAAGCCACT GCACTGTGGT GATTTCGATT ACCACAGGAC CTGCTCTTAT 
TCCTGCAATG TCCAGGGAGC TGTAGTCCAT GACCCTTGCA TTTATTGTTC TGGGGAAATC 
TGCCCTGCTC TGCGAAAAAG AAATATGATG CCCAACTGCT ACAACTGCCG GTGCCATCAC 
CACCAGTGCA TCCGGTGTTC AGCTTGCCAT CATAATCCTC AGCACAGTAC CCTCGAGGAC 
AGCAGCTTGG CACCTGGCAA CACTTGGAAA CCCAGGAAGC CGACAGTGCA GGAATTTCCT 
GGGGACAAAT GGAAACCAAT AACAGGAAAC AGGAAGACCA GCCAGTCAGG GAGGGAAATG 
GCTCATTCCA AGGCTAGCTT TTCATGGGCA ACCCCCTTCC ATCCTTGCAC TGAGAACCCA 
GCACTCGACT TGTCAAGCTA CCGAGCAATC TCTTCTCTTG ACCTCCTGGG AGACTTCAAA 
CACTCCTTGA AAAAAACAGA GGAAACTTCA GTATATGAGG AGGGGAGCTC CATGGCCTCC 
GTGCCCCGGC CACTACGCAG CCGTGCCTTC TCAGAGAGCC ATATCAGCTT GGAGCCCCAG 
AGCTCCCGGG CCTGGGGGCA GCATAGGAGG GAGATCTTTA CCAAAGTTGA TGAGACCCAG 
CCGGATCCTC TGGGAGCCAG GAAGAAGGCC TTTCCTCCTC CTCGCCCTCC TCCTCCCAAC 
TGGGAGAAGT ACAGGCTTTT CCGTGCAGCC CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAAC AGCAGCAACA GCAGCAACAG CAACAGCAGC AACAGCAACA GCAACAGCAG 
CAACAGCAAC AGCAGCAGCA AGAGGAGGAG GAAGAAGAGG AGGAGGAAGA GGAGGAAGAA 
GAGGAAGAGG GAGTAGAAGA GGAGGAAGAG GAGGGGGAGG AGGAGGAGCT GCCACCCCAG 
TATTTCAGTT CAGAAACTAC TAGTTCCTGT ACTCTCAATC CTGAGGAGGT CCTGGTGCAG 
GCACAACCCA TGGCCTTTGG CCATTTGGAG ACCTCAAGGC AGGGCTCACA AAGCCTTCCA 
GCAGAGCAAG AGTCTTTTGT TCACCATTCC AATGATTTCC TGCCTTCAAT AAGGGGCCAC 
TTGGGATCTC AACCTGAGAA GGCTCAGCCC CCTTGCTATT ATGGCATTGG TGGGCTTTGG 
AGGACATCAG AGCAGGAGAC CACTGATTCC CCCAAACCAG AAGCCTTGAT GACAGAAGAG 
TCCAAACCCA AGCCAGCATG GAGCCAGAGC TACTTCTTTC CTGAGGAGCA GTTCTCTTTT 
ATGGGCTGGG GCTCTGAGAA GCAACACCTC AGCCCCGCGG GCTTCGGTGA ACCCAAGACA 
ACAGGACAAG AGTTTCAGCA CTTCTCGCCT CCTCAGGGGG CCCCAGGGAT CCCTACCTCT 
TACTCAGCTT ATTACAATAT TTCTGTGGCC AAGGCAGAGC TGCTGAACAA GTTGAAAGAC 
CAGCCCGAGA TGGTAGAGAT TGGTCTGGGA GAAGAGGAAG TTGACCATGA ACTGGCTCAA 
AAAAAGATAC AGCTTATTGA AAGCATTGGC AGAAAACTTT CTGTCCTGCG GGAGGCCCAG 
CGAGGACTGC TGGAGGACAT CAGTGCCAAT TCTGCCCTTG GGGAGGAGGT GGAAGCCAAC 
CTGAAAGCCG TCTGCAAATC CAATGAGTTT GAAAAATATC ATTTGTTTAT CGGGGACCTA 
GATAAAGTGG TCAACCTGTT GCTGTCACTG TCTGGACGAC TGGCCCGGGT GGAGAATGCT 
CTCAACAGCA TCGATTCAGA GGCCAACCAG GAGAAGTTGG TGCTGATAGA GAAAAAGCAG 
CAGTTGACAG GGCAGTTGGC AGATGCCAAG GAGCTGAAGG AGCACGTGGA CCGTCGGGAG 
AAGTTGGTGT TTGGTATGGT CTCCCGCTAC CTGCCTCAAG ACCAGCTCCA AGATTACCAG 
CACTTTGTAA AGATGAAATC TGCCCTCATC ATTGAACAGC GAGAGCTGGA AGAGAAGATC 
AAGCTCGGGG AAGAGCAGCT CAAATGTCTC AGGGAGAGCC TACTCCTGGG GCCCAGCAAT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHROOM4 ( XM_023633893.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023489661.1 Equus caballus shroom family member 4 (SHROOM4), transcript variant X1, mRNA.

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CloneID	OHb71867
Clone ID Related Accession (Same CDS sequence)	XM_023633893.1
Accession Version	XM_023633893.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	protein Shroom4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009175.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACC GGCCTGGGTC CTTCCAGTAC GTCCCTGTGC AGCTGCAAGG GGGGGCGCCC 
TGGGGTTTCA CCCTTAAGGG GGGTCTGGAA CACTGCGAAC CGCTCACAGT GTCTAAGATT 
GAAGATGGGG GCAAGGCAGC CTTGTCCCAG AAGATGAGGA CTGGTGATGA GCTGGTGAAT 
ATCAATGGCA CTCCATTGTA TGGCTCCCGC CAAGAGGCCC TCATTCTCAT CAAAGGCTCC 
TTCCGGATCC TCAAGCTGAT TGTCAGGAGG AGAAACGCCC CTGTCAGTAG GCCGCACTCA 
TGGCATGTGG CCAAGCTGCT GGAGGGATGC CCTGAAGCGG CCACCACCAT GCATTTCCCT 
TCTGAAGCCT TCAGCTTGTC CTGGCATTCC GGCTGCAACA CAAGTGACGT GTGTGTGCAG 
TGGTGTCCAC TCTCCCGGCA CTGTAGCACT GAGAAAAGCA GCTCCATTGG CAGCATGGAG 
AGCCTGGAGC AACCAAGCCA AGCCACCTAT GAGGGCCACC TCTTGCCCAT TGACCAGAAC 
ATGTACCCCA ACCAGCGTGA CTCAGCCTAC AGCTCCTTCT CGGCCAGCTC AAATGCCTCT 
GACTGTGCCC TTTCCCTCAG GCCAGAGGAG CCAGCCTCTG CTGACTGCAT CGTGCAAGGC 
CCAGGGCCAA CTAAGGCCCC TAATGGCCGG CCTAATGTGG CTGACACCTC AGGAGGTAGC 
CAGCGCACCA ATGGGGGCCA CCTGACTTCC AGCTCCCAGA TGTCATCCTG CCCACAGGAG 
GTCCACCACT CAGGGCCTGC TAAGACTACC AGGGGCCCAC CTCAACCTCC AGTGAGGCGA 
GACAGTCTTC AGGCCTCCAG AGCCCAGCTC CTCAATGGAG AGCAGCGCAG GGCTTCTGAA 
CCTGTGAACT CCTTGCAACA GAAGGAGAAA CCAAGCTTAG AAACCATTCT ATTCCCAAGA 
AACCCTAACA GGTTCTGCTG CCTCAGTGGG CAAGATCAAG TGACAAATGA GGGCCATCAG 
AACTGTGAAC TCAGCCAAAC TCCTGAACCC AGCCAGCAGG GCTCTGAGCA TCTACTGATG 
GAGGCCTCAG CCAAAGCTGT TGGATTCCCA AAAGCATATG ACAAAGCTTC CAGCGTAGAT 
TCCAGCCCAC TCAACAAGGC TTCAGCAGAG CTAGCTAAGG CTTCTTCTTT TGGCAGCACT 
CTACACCTCA CAGGGCATCG CCATAGTGCG CCTGAACAGC TGCTGGCATC CCACTTACAG 
CGTGTGCACC TTGATACCAG GGGCAGCAAG GGAATAGAGC TCCCAACTGG GCAGGATGGG 
CATGAGTGGA CTCTGTCTCC TTTGCATAGC AGCCACACAG AGAAGAAAAG TCCATGTCCG 
TCTGCAGGAG GAACCCAGGA CCAGCCCAGC AAAGAGAGAA AGACTAGGCC AGTGGATGAT 
AGGCCTTTAG GTTCAGGCCA CCAGAGCCCA AGCAGTTCTC CACATGGAGA GGCTGATGGA 
CACCCTCCAG AAAGAGGTTT CCAGGACCCA AACAGAGTAG GCAGTGAATT GGCCATCCAG 
CAGCCCTCTG CTTCTGACTC GCTTATTCAA CAAACCAGGG ACTGTTCTGC AACCACTAAA 
GTACCTGGCA GCACAGAGGC AACTGAAGAA GGGGACAAGG AGCCCAAGGA GTGTGGCCGG 
GTGGGTGGTA GGCGAAATGG AGGGACCCGG GGCCGCTCAA TCCAAAACCG GCGGAAGAGT 
GAGCGTTTTG CTACCAATCT GCGTAATGAA ATTCAGAGGA GGAAGGCTCA GCTCCAGAAA 
AGCAAGAATC CCTTGTCACA GCTGTGTGAC ACTAAGGAGC CAGTGGAAGA GACCGAGGAG 
CCCCTAGAAA GTCCTTCACT TCCCCCCTCT AACTCATCTC TTCTATCTTC ATATAAAAAA 
CCACCTAGCC CCAGAGACAA GCTCTTCAAC AAGAGCATGA TTCTCAGGGC TAGGTCTTCA 
GAGTGCCTCA GCCAAGCCCC TGAGAGCCAT GAATCTAGGA CAGGCTTGGA GGGACAAATA 
AGCCCTGGCC AGAGGCCCGG CCAGGCCTCT TTGGGCCTGA ATACCTGGTG GAAAGCATCT 
GACCCATCCT CCTCAGACTC TGAGAAAACA GATGTTCACT GTGGAGTCCG TGGAGGTCAA 
TGGAGATGGT CTCCAGAGCA CAACCTGCAG CCACATGTGG CACTAGCCAT GGAAGGCCCT 
TCCAACCCAG GTGACAACAA AGAATTGAAG GCTTCTACTT CCCAAGCTGG AGAGGAAACC 
ATCCTCTTGC CCTTTGCAGA CAGAAGAAAA TTCTTTGAAG AGAGTAGCAA ATCCGTATCT 
ACATCTCATT TGCCAGGTTT AACCACACAT GGCAACAAGA CTTTTACCCA GAGACCAAAA 
CCTATAGACC GAAACTTCCA GCCAATGAGC TCCACCTATA GGGAATTGAG GCGCCATCCC 
ATGGACCAAC CATATCATGC CACAGACCAA TCATATCATT CCATGTCACC CCTTCAGTCA 
GAAACTCCCA CTTACTCCGA ATGTTTTGTG AGCAAGGGTC TAGAACAATC CATGTGTTGT 
AAGCCACTGC ACTGTGGTGA TTTCGATTAC CACAGGACCT GCTCTTATTC CTGCAATGTC 
CAGGGAGCTG TAGTCCATGA CCCTTGCATT TATTGTTCTG GGGAAATCTG CCCTGCTCTG 
CGAAAAAGAA ATATGATGCC CAACTGCTAC AACTGCCGGT GCCATCACCA CCAGTGCATC 
CGGTGTTCAG CTTGCCATCA TAATCCTCAG CACAGTACCC TCGAGGACAG CAGCTTGGCA 
CCTGGCAACA CTTGGAAACC CAGGAAGCCG ACAGTGCAGG AATTTCCTGG GGACAAATGG 
AAACCAATAA CAGGAAACAG GAAGACCAGC CAGTCAGGGA GGGAAATGGC TCATTCCAAG 
GCTAGCTTTT CATGGGCAAC CCCCTTCCAT CCTTGCACTG AGAACCCAGC ACTCGACTTG 
TCAAGCTACC GAGCAATCTC TTCTCTTGAC CTCCTGGGAG ACTTCAAACA CTCCTTGAAA 
AAAACAGAGG AAACTTCAGT ATATGAGGAG GGGAGCTCCA TGGCCTCCGT GCCCCGGCCA 
CTACGCAGCC GTGCCTTCTC AGAGAGCCAT ATCAGCTTGG AGCCCCAGAG CTCCCGGGCC 
TGGGGGCAGC ATAGGAGGGA GATCTTTACC AAAGTTGATG AGACCCAGCC GGATCCTCTG 
GGAGCCAGGA AGAAGGCCTT TCCTCCTCCT CGCCCTCCTC CTCCCAACTG GGAGAAGTAC 
AGGCTTTTCC GTGCAGCCCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAACAG 
CAGCAACAGC AGCAACAGCA ACAGCAGCAA CAGCAACAGC AACAGCAGCA ACAGCAACAG 
CAGCAGCAAG AGGAGGAGGA AGAAGAGGAG GAGGAAGAGG AGGAAGAAGA GGAAGAGGGA 
GTAGAAGAGG AGGAAGAGGA GGGGGAGGAG GAGGAGCTGC CACCCCAGTA TTTCAGTTCA 
GAAACTACTA GTTCCTGTAC TCTCAATCCT GAGGAGGTCC TGGTGCAGGC ACAACCCATG 
GCCTTTGGCC ATTTGGAGAC CTCAAGGCAG GGCTCACAAA GCCTTCCAGC AGAGCAAGAG 
TCTTTTGTTC ACCATTCCAA TGATTTCCTG CCTTCAATAA GGGGCCACTT GGGATCTCAA 
CCTGAGAAGG CTCAGCCCCC TTGCTATTAT GGCATTGGTG GGCTTTGGAG GACATCAGAG 
CAGGAGACCA CTGATTCCCC CAAACCAGAA GCCTTGATGA CAGAAGAGTC CAAACCCAAG 
CCAGCATGGA GCCAGAGCTA CTTCTTTCCT GAGGAGCAGT TCTCTTTTAT GGGCTGGGGC 
TCTGAGAAGC AACACCTCAG CCCCGCGGGC TTCGGTGAAC CCAAGACAAC AGGACAAGAG 
TTTCAGCACT TCTCGCCTCC TCAGGGGGCC CCAGGGATCC CTACCTCTTA CTCAGCTTAT 
TACAATATTT CTGTGGCCAA GGCAGAGCTG CTGAACAAGT TGAAAGACCA GCCCGAGATG 
GTAGAGATTG GTCTGGGAGA AGAGGAAGTT GACCATGAAC TGGCTCAAAA AAAGATACAG 
CTTATTGAAA GCATTGGCAG AAAACTTTCT GTCCTGCGGG AGGCCCAGCG AGGACTGCTG 
GAGGACATCA GTGCCAATTC TGCCCTTGGG GAGGAGGTGG AAGCCAACCT GAAAGCCGTC 
TGCAAATCCA ATGAGTTTGA AAAATATCAT TTGTTTATCG GGGACCTAGA TAAAGTGGTC 
AACCTGTTGC TGTCACTGTC TGGACGACTG GCCCGGGTGG AGAATGCTCT CAACAGCATC 
GATTCAGAGG CCAACCAGGA GAAGTTGGTG CTGATAGAGA AAAAGCAGCA GTTGACAGGG 
CAGTTGGCAG ATGCCAAGGA GCTGAAGGAG CACGTGGACC GTCGGGAGAA GTTGGTGTTT 
GGTATGGTCT CCCGCTACCT GCCTCAAGAC CAGCTCCAAG ATTACCAGCA CTTTGTAAAG 
ATGAAATCTG CCCTCATCAT TGAACAGCGA GAGCTGGAAG AGAAGATCAA GCTCGGGGAA 
GAGCAGCTCA AATGTCTCAG GGAGAGCCTA CTCCTGGGGC CCAGCAATTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023489661.1
CDS	272..4945
Translation	MENRPGSFQYVPVQLQGGAPWGFTLKGGLEHCEPLTVSKIEDGGKAALSQKMRTGDELVNINGTPLYGSRQEALILIKGSFRILKLIVRRRNAPVSRPHSWHVAKLLEGCPEAATTMHFPSEAFSLSWHSGCNTSDVCVQWCPLSRHCSTEKSSSIGSMESLEQPSQATYEGHLLPIDQNMYPNQRDSAYSSFSASSNASDCALSLRPEEPASADCIVQGPGPTKAPNGRPNVADTSGGSQRTNGGHLTSSSQMSSCPQEVHHSGPAKTTRGPPQPPVRRDSLQASRAQLLNGEQRRASEPVNSLQQKEKPSLETILFPRNPNRFCCLSGQDQVTNEGHQNCELSQTPEPSQQGSEHLLMEASAKAVGFPKAYDKASSVDSSPLNKASAELAKASSFGSTLHLTGHRHSAPEQLLASHLQRVHLDTRGSKGIELPTGQDGHEWTLSPLHSSHTEKKSPCPSAGGTQDQPSKERKTRPVDDRPLGSGHQSPSSSPHGEADGHPPERGFQDPNRVGSELAIQQPSASDSLIQQTRDCSATTKVPGSTEATEEGDKEPKECGRVGGRRNGGTRGRSIQNRRKSERFATNLRNEIQRRKAQLQKSKNPLSQLCDTKEPVEETEEPLESPSLPPSNSSLLSSYKKPPSPRDKLFNKSMILRARSSECLSQAPESHESRTGLEGQISPGQRPGQASLGLNTWWKASDPSSSDSEKTDVHCGVRGGQWRWSPEHNLQPHVALAMEGPSNPGDNKELKASTSQAGEETILLPFADRRKFFEESSKSVSTSHLPGLTTHGNKTFTQRPKPIDRNFQPMSSTYRELRRHPMDQPYHATDQSYHSMSPLQSETPTYSECFVSKGLEQSMCCKPLHCGDFDYHRTCSYSCNVQGAVVHDPCIYCSGEICPALRKRNMMPNCYNCRCHHHQCIRCSACHHNPQHSTLEDSSLAPGNTWKPRKPTVQEFPGDKWKPITGNRKTSQSGREMAHSKASFSWATPFHPCTENPALDLSSYRAISSLDLLGDFKHSLKKTEETSVYEEGSSMASVPRPLRSRAFSESHISLEPQSSRAWGQHRREIFTKVDETQPDPLGARKKAFPPPRPPPPNWEKYRLFRAAQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQEEEEEEEEEEEEEEEEGVEEEEEEGEEEELPPQYFSSETTSSCTLNPEEVLVQAQPMAFGHLETSRQGSQSLPAEQESFVHHSNDFLPSIRGHLGSQPEKAQPPCYYGIGGLWRTSEQETTDSPKPEALMTEESKPKPAWSQSYFFPEEQFSFMGWGSEKQHLSPAGFGEPKTTGQEFQHFSPPQGAPGIPTSYSAYYNISVAKAELLNKLKDQPEMVEIGLGEEEVDHELAQKKIQLIESIGRKLSVLREAQRGLLEDISANSALGEEVEANLKAVCKSNEFEKYHLFIGDLDKVVNLLLSLSGRLARVENALNSIDSEANQEKLVLIEKKQQLTGQLADAKELKEHVDRREKLVFGMVSRYLPQDQLQDYQHFVKMKSALIIEQRELEEKIKLGEEQLKCLRESLLLGPSNF

Target ORF information:

RefSeq Version	XM_023633893.1
Organism	Equus caballus(horse)
Definition	Equus caballus shroom family member 4 (SHROOM4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023633893.1

ORF Insert Sequence:

ATGGAGAACC GGCCTGGGTC CTTCCAGTAC GTCCCTGTGC AGCTGCAAGG GGGGGCGCCC 
TGGGGTTTCA CCCTTAAGGG GGGTCTGGAA CACTGCGAAC CGCTCACAGT GTCTAAGATT 
GAAGATGGGG GCAAGGCAGC CTTGTCCCAG AAGATGAGGA CTGGTGATGA GCTGGTGAAT 
ATCAATGGCA CTCCATTGTA TGGCTCCCGC CAAGAGGCCC TCATTCTCAT CAAAGGCTCC 
TTCCGGATCC TCAAGCTGAT TGTCAGGAGG AGAAACGCCC CTGTCAGTAG GCCGCACTCA 
TGGCATGTGG CCAAGCTGCT GGAGGGATGC CCTGAAGCGG CCACCACCAT GCATTTCCCT 
TCTGAAGCCT TCAGCTTGTC CTGGCATTCC GGCTGCAACA CAAGTGACGT GTGTGTGCAG 
TGGTGTCCAC TCTCCCGGCA CTGTAGCACT GAGAAAAGCA GCTCCATTGG CAGCATGGAG 
AGCCTGGAGC AACCAAGCCA AGCCACCTAT GAGGGCCACC TCTTGCCCAT TGACCAGAAC 
ATGTACCCCA ACCAGCGTGA CTCAGCCTAC AGCTCCTTCT CGGCCAGCTC AAATGCCTCT 
GACTGTGCCC TTTCCCTCAG GCCAGAGGAG CCAGCCTCTG CTGACTGCAT CGTGCAAGGC 
CCAGGGCCAA CTAAGGCCCC TAATGGCCGG CCTAATGTGG CTGACACCTC AGGAGGTAGC 
CAGCGCACCA ATGGGGGCCA CCTGACTTCC AGCTCCCAGA TGTCATCCTG CCCACAGGAG 
GTCCACCACT CAGGGCCTGC TAAGACTACC AGGGGCCCAC CTCAACCTCC AGTGAGGCGA 
GACAGTCTTC AGGCCTCCAG AGCCCAGCTC CTCAATGGAG AGCAGCGCAG GGCTTCTGAA 
CCTGTGAACT CCTTGCAACA GAAGGAGAAA CCAAGCTTAG AAACCATTCT ATTCCCAAGA 
AACCCTAACA GGTTCTGCTG CCTCAGTGGG CAAGATCAAG TGACAAATGA GGGCCATCAG 
AACTGTGAAC TCAGCCAAAC TCCTGAACCC AGCCAGCAGG GCTCTGAGCA TCTACTGATG 
GAGGCCTCAG CCAAAGCTGT TGGATTCCCA AAAGCATATG ACAAAGCTTC CAGCGTAGAT 
TCCAGCCCAC TCAACAAGGC TTCAGCAGAG CTAGCTAAGG CTTCTTCTTT TGGCAGCACT 
CTACACCTCA CAGGGCATCG CCATAGTGCG CCTGAACAGC TGCTGGCATC CCACTTACAG 
CGTGTGCACC TTGATACCAG GGGCAGCAAG GGAATAGAGC TCCCAACTGG GCAGGATGGG 
CATGAGTGGA CTCTGTCTCC TTTGCATAGC AGCCACACAG AGAAGAAAAG TCCATGTCCG 
TCTGCAGGAG GAACCCAGGA CCAGCCCAGC AAAGAGAGAA AGACTAGGCC AGTGGATGAT 
AGGCCTTTAG GTTCAGGCCA CCAGAGCCCA AGCAGTTCTC CACATGGAGA GGCTGATGGA 
CACCCTCCAG AAAGAGGTTT CCAGGACCCA AACAGAGTAG GCAGTGAATT GGCCATCCAG 
CAGCCCTCTG CTTCTGACTC GCTTATTCAA CAAACCAGGG ACTGTTCTGC AACCACTAAA 
GTACCTGGCA GCACAGAGGC AACTGAAGAA GGGGACAAGG AGCCCAAGGA GTGTGGCCGG 
GTGGGTGGTA GGCGAAATGG AGGGACCCGG GGCCGCTCAA TCCAAAACCG GCGGAAGAGT 
GAGCGTTTTG CTACCAATCT GCGTAATGAA ATTCAGAGGA GGAAGGCTCA GCTCCAGAAA 
AGCAAGAATC CCTTGTCACA GCTGTGTGAC ACTAAGGAGC CAGTGGAAGA GACCGAGGAG 
CCCCTAGAAA GTCCTTCACT TCCCCCCTCT AACTCATCTC TTCTATCTTC ATATAAAAAA 
CCACCTAGCC CCAGAGACAA GCTCTTCAAC AAGAGCATGA TTCTCAGGGC TAGGTCTTCA 
GAGTGCCTCA GCCAAGCCCC TGAGAGCCAT GAATCTAGGA CAGGCTTGGA GGGACAAATA 
AGCCCTGGCC AGAGGCCCGG CCAGGCCTCT TTGGGCCTGA ATACCTGGTG GAAAGCATCT 
GACCCATCCT CCTCAGACTC TGAGAAAACA GATGTTCACT GTGGAGTCCG TGGAGGTCAA 
TGGAGATGGT CTCCAGAGCA CAACCTGCAG CCACATGTGG CACTAGCCAT GGAAGGCCCT 
TCCAACCCAG GTGACAACAA AGAATTGAAG GCTTCTACTT CCCAAGCTGG AGAGGAAACC 
ATCCTCTTGC CCTTTGCAGA CAGAAGAAAA TTCTTTGAAG AGAGTAGCAA ATCCGTATCT 
ACATCTCATT TGCCAGGTTT AACCACACAT GGCAACAAGA CTTTTACCCA GAGACCAAAA 
CCTATAGACC GAAACTTCCA GCCAATGAGC TCCACCTATA GGGAATTGAG GCGCCATCCC 
ATGGACCAAC CATATCATGC CACAGACCAA TCATATCATT CCATGTCACC CCTTCAGTCA 
GAAACTCCCA CTTACTCCGA ATGTTTTGTG AGCAAGGGTC TAGAACAATC CATGTGTTGT 
AAGCCACTGC ACTGTGGTGA TTTCGATTAC CACAGGACCT GCTCTTATTC CTGCAATGTC 
CAGGGAGCTG TAGTCCATGA CCCTTGCATT TATTGTTCTG GGGAAATCTG CCCTGCTCTG 
CGAAAAAGAA ATATGATGCC CAACTGCTAC AACTGCCGGT GCCATCACCA CCAGTGCATC 
CGGTGTTCAG CTTGCCATCA TAATCCTCAG CACAGTACCC TCGAGGACAG CAGCTTGGCA 
CCTGGCAACA CTTGGAAACC CAGGAAGCCG ACAGTGCAGG AATTTCCTGG GGACAAATGG 
AAACCAATAA CAGGAAACAG GAAGACCAGC CAGTCAGGGA GGGAAATGGC TCATTCCAAG 
GCTAGCTTTT CATGGGCAAC CCCCTTCCAT CCTTGCACTG AGAACCCAGC ACTCGACTTG 
TCAAGCTACC GAGCAATCTC TTCTCTTGAC CTCCTGGGAG ACTTCAAACA CTCCTTGAAA 
AAAACAGAGG AAACTTCAGT ATATGAGGAG GGGAGCTCCA TGGCCTCCGT GCCCCGGCCA 
CTACGCAGCC GTGCCTTCTC AGAGAGCCAT ATCAGCTTGG AGCCCCAGAG CTCCCGGGCC 
TGGGGGCAGC ATAGGAGGGA GATCTTTACC AAAGTTGATG AGACCCAGCC GGATCCTCTG 
GGAGCCAGGA AGAAGGCCTT TCCTCCTCCT CGCCCTCCTC CTCCCAACTG GGAGAAGTAC 
AGGCTTTTCC GTGCAGCCCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAACAG 
CAGCAACAGC AGCAACAGCA ACAGCAGCAA CAGCAACAGC AACAGCAGCA ACAGCAACAG 
CAGCAGCAAG AGGAGGAGGA AGAAGAGGAG GAGGAAGAGG AGGAAGAAGA GGAAGAGGGA 
GTAGAAGAGG AGGAAGAGGA GGGGGAGGAG GAGGAGCTGC CACCCCAGTA TTTCAGTTCA 
GAAACTACTA GTTCCTGTAC TCTCAATCCT GAGGAGGTCC TGGTGCAGGC ACAACCCATG 
GCCTTTGGCC ATTTGGAGAC CTCAAGGCAG GGCTCACAAA GCCTTCCAGC AGAGCAAGAG 
TCTTTTGTTC ACCATTCCAA TGATTTCCTG CCTTCAATAA GGGGCCACTT GGGATCTCAA 
CCTGAGAAGG CTCAGCCCCC TTGCTATTAT GGCATTGGTG GGCTTTGGAG GACATCAGAG 
CAGGAGACCA CTGATTCCCC CAAACCAGAA GCCTTGATGA CAGAAGAGTC CAAACCCAAG 
CCAGCATGGA GCCAGAGCTA CTTCTTTCCT GAGGAGCAGT TCTCTTTTAT GGGCTGGGGC 
TCTGAGAAGC AACACCTCAG CCCCGCGGGC TTCGGTGAAC CCAAGACAAC AGGACAAGAG 
TTTCAGCACT TCTCGCCTCC TCAGGGGGCC CCAGGGATCC CTACCTCTTA CTCAGCTTAT 
TACAATATTT CTGTGGCCAA GGCAGAGCTG CTGAACAAGT TGAAAGACCA GCCCGAGATG 
GTAGAGATTG GTCTGGGAGA AGAGGAAGTT GACCATGAAC TGGCTCAAAA AAAGATACAG 
CTTATTGAAA GCATTGGCAG AAAACTTTCT GTCCTGCGGG AGGCCCAGCG AGGACTGCTG 
GAGGACATCA GTGCCAATTC TGCCCTTGGG GAGGAGGTGG AAGCCAACCT GAAAGCCGTC 
TGCAAATCCA ATGAGTTTGA AAAATATCAT TTGTTTATCG GGGACCTAGA TAAAGTGGTC 
AACCTGTTGC TGTCACTGTC TGGACGACTG GCCCGGGTGG AGAATGCTCT CAACAGCATC 
GATTCAGAGG CCAACCAGGA GAAGTTGGTG CTGATAGAGA AAAAGCAGCA GTTGACAGGG 
CAGTTGGCAG ATGCCAAGGA GCTGAAGGAG CACGTGGACC GTCGGGAGAA GTTGGTGTTT 
GGTATGGTCT CCCGCTACCT GCCTCAAGAC CAGCTCCAAG ATTACCAGCA CTTTGTAAAG 
ATGAAATCTG CCCTCATCAT TGAACAGCGA GAGCTGGAAG AGAAGATCAA GCTCGGGGAA 
GAGCAGCTCA AATGTCTCAG GGAGAGCCTA CTCCTGGGGC CCAGCAATTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SHROOM4 cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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