LOC6637983 cDNA ORF clone, Drosophila willistoni

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC6637983
Entrez Gene ID	6637983
Full Name	endoribonuclease Dicer
Synonyms	GK10670,GK10670-PA
Gene Type	protein-coding
Organism	Drosophila willistoni

mRNA and Protein(s)

mRNA	Protein	Name
XM_002060985.2	XP_002061021.2	uncharacterized protein
XM_002060985.3	XP_002061021.2	endoribonuclease Dicer
XM_023175056.1	XP_023030824.1	endoribonuclease Dicer

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC6637983 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC6637983 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODf22014	XM_023175056.1 Latest version!	Drosophila willistoni endoribonuclease Dicer (LOC6637983), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODf22014	XM_002060985.3 Latest version!	Drosophila willistoni endoribonuclease Dicer (LOC6637983), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODf22014	XM_002060985.2	Drosophila willistoni uncharacterized protein (Dwil\GK10670), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC6637983 ( XM_002060985.2 ) cDNA ORF clone, Drosophila willistoni -> XP_002061021.2 Drosophila willistoni uncharacterized protein (Dwil\GK10670), mRNA.

Price & Availability↑

CloneID	ODf22014
Clone ID Related Accession (Same CDS sequence)	XM_002060985.2 , XM_002060985.3 , XM_023175056.1
Accession Version	XM_002060985.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila willistoni
Product	uncharacterized protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002032340). On Dec 21, 2015 this sequence version replaced XM_002060985.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTGCTA ACGCGATGAC AACCCCACCC AAGGAAATGC AAGTACGTAG TTATCAATCG 
CGTCTGGTTG ACTATCTAAC TGAGCGCAAT GGGATAGTCT ATTTGCCCAC AGGAGCCGGC 
AAAACCTTTG TGGCCATCCT GACACTAAAA CGTTTCTCAT TGGACTTTGA TAAAACCATC 
GAGGATGGCG GAAAACGAGC TATATTCATG TGCAACACTG TCGAATTAGC CCGTCAACAG 
GCATTGGCTT GTAGATCACT TACAAATTTC AAAGTTGGAT TTTATGTAGG CGATCAAGGT 
GTCGATGAAT GGAGTAGATC CAAATGGGAT GTTGAAATAA GAGAAAAACA AGTTCTTGTT 
GGCACTGCCC AGGTCATGTT GGACCTGATG CTTCACGCCC ATCTGTCTCT GGGCTCCGTA 
AGCATTGTGA TTATAGATGA ATGTCATCAT GGCACCGGAC GTCATCCGTT TCGTGAATTT 
ATGCGTTTAT TTCTACACGT TCGGCCTAAT GTGCAACTGC CACGAGTTGT TGGGCTTACC 
GGAGTCTTGA TCAAGGGCAA TGAGATCAAC AATGTGAAAA AAATGTTGAA AAATCTGGAG 
ACAACATATC ATGGCAATAT TATCACCGTG TCCGATATGA AAGAAATGGA GAATGTCATG 
CTTTACTCGA CCAAGCCCAA GGAGTTTTTT TTGTTATATG GCTATGGAAC TCATAAATTT 
GAGCTCATAG ATACTATTAA ATATTACATT GAAGCGTTTC ACAAGAAGTT GGACAATTAC 
GATATTGGCA AACAACCAGT CCGTTTCTCC AATGGTCTGC AGCAGCAGCG GGAGCCAAAC 
AAGAAAAATT TTGCAAAAAG TCTACTTAAT GATTTTATTT ATCAACTGGA AGAGTATGGT 
ATCTACGTGG CGTCTATAGC AGTCGTTGCT TTACTAGTTG AGTTTGAAAT AAAGAAACGA 
CAGGCCGAGA CGCTGGTCTT AAGATCAATA TACCGAATGG CCATTAGTCT ATGTGATAAT 
ATTCGACATT TCCTTGTAAG CAAATTGAGG GAAGACATAG ATGATGATAT GTCTGATGAC 
AATGTGGACA CCGAAGAAAA CATTTACAAT TTTTCTACCC CTCGAGTGCA AAGATTTCTT 
CATTATTTAA AGCAAACCTT TAGCAACAAG AATCCAAAGG ATATATGCTG TCTAGTATTT 
GTAGAACGAA GGTACACATG TAAGAGCATC TACGGTATGC TTCTTAAGTA TATCGCCTCA 
GTGCCGGAGT TGAAGAATGT ACTGGTGCCG CAGTTTATGG TGGGACGGAA TGGAATCAAT 
CAAAATTTCG ATACCGTACT GGAACGAAAA TGGCAAAACT CAGCCATTCA ACAATTTCGA 
GATGGCGAGG CAAATCTAAT GATATGCTCA TCCGTATTAG AGGAGGGCAT CGATGTGCAG 
GCCTGTAATT ATGTTATTAT ACTGGATCCA TTGAAGACAT TTAACTCATA TGTGCAAACA 
AAGGGACGGG CTCGGTCTAA AGATGCTGTT TTTCTTATCC TAGCGTCGGA ATCTGAAAAG 
CCAAAGACTG CGAAGAGAAT TATGGACTAT CAGAATGCTC ATAGAGATAT AGCCAATTAT 
CTGCAGGATC GTGTCCTGGA GCGTGCCGAG CCAAAAATGG ATGAAATAAA CGAACATTTT 
AGAGATATAA TTCCGCCTTA TATCAATGAG ACAGGTTCTT TGCTTCTGGC CAGTAGTGCG 
CTAGCATTGC TACATCGTTA TTGTCAAACC TTACCATCCG ATGCATTTGG TTTCAATATG 
CCGTGGTACA AATTGCTTAA CCCAGACGAA CGTGTGCAAC ATTTTGGCAA AAGTGCTGAA 
ACTAAGCAAG TGGTTTCCAT TAATCTGCCA CTTAGCACCA AGCTAAGGAG TACCATATAT 
AGTGATCTTA TGAATTGCAA TAAATCAGCT AAAATTTCGG CTGCCTTCAA TGTGTGTAAG 
GAACTTTATA AACTTGGAGA GCTCAATCAA AAATTTCTGC CAACTACATT AACTGACCGC 
CTAAAAGATG TTTCTGATAT CCATTTTAAG CACTGGAAGA AATACGGCGA CGATGTAACC 
AAAACAAACC GTAAGGAAGA TAAATCAGGC AATACCAGCA AATATCCTGT CAACTGTCCC 
ACCCAATTGT ATGATACACG TCCTCGTGTG GGTGAGACCT GTTATGCTTA TGAGATATTA 
TTGAAGCCAC ATTTCGAGCG CAATGAATAC ACGGCCCATA TGCACGATGC CTTACAGACG 
GGGCGTGGGT ATGCCTTGCT CTTGCGTAAG CGACTACCAC CTCTGGCCGA AATGCCATTG 
TTCAGCAGTC ATGGAAAGAT TCATGTATCG GTCGCTGAGC AGCCGCATGA AGTGATTTTG 
AAAACTGAAG AGCAATTGGA TCATCTGCAT CAATTCCATA TTATGCTGTT TAGGGATATC 
TTGAAAATTT GGCAGTCCTA CTTTGTGCTT GATAAACGCA GCAAGGAGAA TTCCTATCTG 
GTGGTCCCCT TGGATATTAA ATCCAGCGGA AAAGGGGAGG GCAATATAAT AGATTGGAAT 
CTAGTGACGC AGTTCTATCG TGGTCTACCA CAATCAAGAA CTCTAAGCCC GGATCAGAGA 
CGCAGGCAAG AACCACCATG CCCGGATGAC TTTGAGGGTA AAATTGTGAC CCAATGGTAT 
GCAAGTTATC AAGATAAACG GATGTTGGTC ACCAAGGTGC ACAGGGATAA GACCCCATTT 
AGCATGATGG AAAGTAACCA AAGCGAAAAT AATTATTATG ACTACACCAT GTCCAAGTAT 
AAAAATGTTA TCGGAGATGT GGTCCATCAA AATCAGTGCC TTATCGAAGT TCGTGAGTTA 
ACACAGCAAT TAAATTTCTA TATAGCACAT CGAGTAAAAA CGTCGGGTCA GAGCAAAGCT 
CGTGCGCGAA TTATTTTAAT ACCCGAGCTA TGCTTCAATT TTCAATTTCC CGGCGATCTT 
TGGGCAAAAT TACTCTTTCT GCCAAGCATT CTGAATCGTT TGCATTACCT GCTTCATGCA 
GAGGCACTGC GTCAGCGTAT GAATACTTAC TTGGGACTAC ATGTGCTACC ACAAAATGGT 
CCAAATTATC GGCCCAAAGT GCTGGATGTC GATTTGTCGT TGCGCCGCAA TGTGGATTCT 
ACAGGAAATG CAGTTAGCAT GGAAGTTGAG GAACCACGCT CCATGTTGGA TCCATTGCCC 
ACCAAACAAT TAGAGGTATC AATGGATCGT CTTCAGATTA GTGATCTAGA AGCACCCTGG 
CAAAGTTATA TGGAACCAGT AGACTTGAGT CGCAACATGA TGTTGACTCA TCCAGTTGAA 
CTTGCTTATT ACTGTCAATT CACCAGCGGC CAGATTGTCA AATTTGACAA GATAGAGGAA 
GAGGACAAAG AATTCTGGAT GGAGACGCAA TTCAAAATGT CCAAGGGAAA TATTTACGAC 
TATAACACTA CGGCGAACGA ATCTCTACCA GCCCTGCTAC CCAACTCGAA TCCAATAACT 
CCAGTCGAGC AGCTATCGGT GTTGCAGAAA ACCATTTCCA ATGAACACAT CACTCCCGCC 
GAGCAGAGTG AATTTCTAAC AGCGATTACA AGTTCCGGAT GCTCGGATGT CTTTGATATG 
GAGCGTTTGG AATTGTTGGG TGATAGTTTT CTAAAGATGA GTGCAACATT ATATTTGTCA 
AGCATTCATA GCAACTGGAA CGAAGGCGTT CTCACCCAAA TAAAATCCAC ATTGGTGTCC 
AATCGCAATC TTTTATATTG CATGAACGAA ACCGATATTC CTTCGAGAAT CAATAGCACC 
TTGTTCACTC CCAAGTACAC CTGGAAACCG CCTGGCCTTA GTTTGCCACA TAATATATTA 
AATCTATGGC AGGAACAGCC AGAAGTCGCC AAATTTGTAT TACCAAGAAA TCTGTGTGCC 
CTACAACTTA CTGAGGAAGA GATTTTCTCA TTGGGAAAAT TAACCCCGGC AAACTTTGAA 
AAGTTTTTGG AAAGCTGTCA GGCCAACACG AATTACACCA GTACGGATTA TGAATCTGAA 
ATGAATTTCT GTATCGGTAA ATCAAAACTG TCAAACAAAG TGGTCGCCGA CACGTTGGAA 
GCTCTCCTTG GCGTTATTGT TAAGAACTAT GGTGTTCAGC ATGGTTTCCG CATTTTGGAA 
TATTTTGGCA TCTGCAAGCA GGTAGACATT GGCAAGCCAT TGTCCCAACT TCTTGACCTT 
CAATTGGGTA GCACTCGTAC ACGAGCGGAT CGTGATCCTC GTGAGATTGA TGGACTACTC 
ATCAATTATC ATTATTTGGA GCGAAATCTT GGATACACCT TCAAAGATCG CGCCTATTTA 
CTACAGGCTC TCACACATCC ATCGAATCCC ACTAACCGTT TGACTGGCTG CTATCAAGAG 
TTGGAGTTTA TTGGTGATGC CATTTTGGAT TTCCTAATCT CAGCATATAT TTTTGAGCGA 
AATATCCGCA TGAATCCTGG CCAACTGACA GATTTGCGAT CCGCTTTGGT CAATAACACA 
ACATTGGCCT GCATTTGTGT TAGGCATCGG TTCCATTTAT TCATTTTGGC TGAAAATACA 
CTACTTACCG AATCCATCAA TAGTTTTGTG GATTTCCAGG AAAAACATAT GCACCGTGTA 
ACCCATCATG TGCGAATACT TCTTGAAGAA AACGATATAC AATGTCACAA TCCCTTGGAC 
GAGGAAGATG AATTGGAAAT GGCCGACAAT TTGTCTACCA ACGATGAACC GCTAGCCAAG 
GGTAACTTGA ATATGTCGGA AAATGTAGAT GTGCCCAAGG CCTTGGGTGA TGTTGTAGAA 
GCCTTAATTG CAGCCGTGTA TCTTGATTGC AAGGATTTAC AAAAAACCTG GGAAGTGGTA 
TACAGACTCT TTGAGCCTGA ACTTATTGAA TTCTCACGCA ATGTGCCTAT AAATCCTCTT 
CGTCAACTCA ATGAGCATAG GCTAGCTAAA CCGATATTCA GTCCTGCCAT TATGGATGAG 
GGTAAGGTGA TGGTTAGTTG CTTATTCACC TGCAATGAGA AAACCATTAA GGTCTATGGA 
TTTGGTACCA ACAACAGCCA AGCTAAGCTG GCGGCAGCAA AACACGCTCT ACGCAAACTT 
GCCAAGAGCG AAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002061021.2
CDS	38..5215
Translation	MPANAMTTPPKEMQVRSYQSRLVDYLTERNGIVYLPTGAGKTFVAILTLKRFSLDFDKTIEDGGKRAIFMCNTVELARQQALACRSLTNFKVGFYVGDQGVDEWSRSKWDVEIREKQVLVGTAQVMLDLMLHAHLSLGSVSIVIIDECHHGTGRHPFREFMRLFLHVRPNVQLPRVVGLTGVLIKGNEINNVKKMLKNLETTYHGNIITVSDMKEMENVMLYSTKPKEFFLLYGYGTHKFELIDTIKYYIEAFHKKLDNYDIGKQPVRFSNGLQQQREPNKKNFAKSLLNDFIYQLEEYGIYVASIAVVALLVEFEIKKRQAETLVLRSIYRMAISLCDNIRHFLVSKLREDIDDDMSDDNVDTEENIYNFSTPRVQRFLHYLKQTFSNKNPKDICCLVFVERRYTCKSIYGMLLKYIASVPELKNVLVPQFMVGRNGINQNFDTVLERKWQNSAIQQFRDGEANLMICSSVLEEGIDVQACNYVIILDPLKTFNSYVQTKGRARSKDAVFLILASESEKPKTAKRIMDYQNAHRDIANYLQDRVLERAEPKMDEINEHFRDIIPPYINETGSLLLASSALALLHRYCQTLPSDAFGFNMPWYKLLNPDERVQHFGKSAETKQVVSINLPLSTKLRSTIYSDLMNCNKSAKISAAFNVCKELYKLGELNQKFLPTTLTDRLKDVSDIHFKHWKKYGDDVTKTNRKEDKSGNTSKYPVNCPTQLYDTRPRVGETCYAYEILLKPHFERNEYTAHMHDALQTGRGYALLLRKRLPPLAEMPLFSSHGKIHVSVAEQPHEVILKTEEQLDHLHQFHIMLFRDILKIWQSYFVLDKRSKENSYLVVPLDIKSSGKGEGNIIDWNLVTQFYRGLPQSRTLSPDQRRRQEPPCPDDFEGKIVTQWYASYQDKRMLVTKVHRDKTPFSMMESNQSENNYYDYTMSKYKNVIGDVVHQNQCLIEVRELTQQLNFYIAHRVKTSGQSKARARIILIPELCFNFQFPGDLWAKLLFLPSILNRLHYLLHAEALRQRMNTYLGLHVLPQNGPNYRPKVLDVDLSLRRNVDSTGNAVSMEVEEPRSMLDPLPTKQLEVSMDRLQISDLEAPWQSYMEPVDLSRNMMLTHPVELAYYCQFTSGQIVKFDKIEEEDKEFWMETQFKMSKGNIYDYNTTANESLPALLPNSNPITPVEQLSVLQKTISNEHITPAEQSEFLTAITSSGCSDVFDMERLELLGDSFLKMSATLYLSSIHSNWNEGVLTQIKSTLVSNRNLLYCMNETDIPSRINSTLFTPKYTWKPPGLSLPHNILNLWQEQPEVAKFVLPRNLCALQLTEEEIFSLGKLTPANFEKFLESCQANTNYTSTDYESEMNFCIGKSKLSNKVVADTLEALLGVIVKNYGVQHGFRILEYFGICKQVDIGKPLSQLLDLQLGSTRTRADRDPREIDGLLINYHYLERNLGYTFKDRAYLLQALTHPSNPTNRLTGCYQELEFIGDAILDFLISAYIFERNIRMNPGQLTDLRSALVNNTTLACICVRHRFHLFILAENTLLTESINSFVDFQEKHMHRVTHHVRILLEENDIQCHNPLDEEDELEMADNLSTNDEPLAKGNLNMSENVDVPKALGDVVEALIAAVYLDCKDLQKTWEVVYRLFEPELIEFSRNVPINPLRQLNEHRLAKPIFSPAIMDEGKVMVSCLFTCNEKTIKVYGFGTNNSQAKLAAAKHALRKLAKSEA

Target ORF information:

RefSeq Version	XM_002060985.2
Organism	Drosophila willistoni
Definition	Drosophila willistoni uncharacterized protein (Dwil\GK10670), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002060985.2

ORF Insert Sequence:

ATGCCTGCTA ACGCGATGAC AACCCCACCC AAGGAAATGC AAGTACGTAG TTATCAATCG 
CGTCTGGTTG ACTATCTAAC TGAGCGCAAT GGGATAGTCT ATTTGCCCAC AGGAGCCGGC 
AAAACCTTTG TGGCCATCCT GACACTAAAA CGTTTCTCAT TGGACTTTGA TAAAACCATC 
GAGGATGGCG GAAAACGAGC TATATTCATG TGCAACACTG TCGAATTAGC CCGTCAACAG 
GCATTGGCTT GTAGATCACT TACAAATTTC AAAGTTGGAT TTTATGTAGG CGATCAAGGT 
GTCGATGAAT GGAGTAGATC CAAATGGGAT GTTGAAATAA GAGAAAAACA AGTTCTTGTT 
GGCACTGCCC AGGTCATGTT GGACCTGATG CTTCACGCCC ATCTGTCTCT GGGCTCCGTA 
AGCATTGTGA TTATAGATGA ATGTCATCAT GGCACCGGAC GTCATCCGTT TCGTGAATTT 
ATGCGTTTAT TTCTACACGT TCGGCCTAAT GTGCAACTGC CACGAGTTGT TGGGCTTACC 
GGAGTCTTGA TCAAGGGCAA TGAGATCAAC AATGTGAAAA AAATGTTGAA AAATCTGGAG 
ACAACATATC ATGGCAATAT TATCACCGTG TCCGATATGA AAGAAATGGA GAATGTCATG 
CTTTACTCGA CCAAGCCCAA GGAGTTTTTT TTGTTATATG GCTATGGAAC TCATAAATTT 
GAGCTCATAG ATACTATTAA ATATTACATT GAAGCGTTTC ACAAGAAGTT GGACAATTAC 
GATATTGGCA AACAACCAGT CCGTTTCTCC AATGGTCTGC AGCAGCAGCG GGAGCCAAAC 
AAGAAAAATT TTGCAAAAAG TCTACTTAAT GATTTTATTT ATCAACTGGA AGAGTATGGT 
ATCTACGTGG CGTCTATAGC AGTCGTTGCT TTACTAGTTG AGTTTGAAAT AAAGAAACGA 
CAGGCCGAGA CGCTGGTCTT AAGATCAATA TACCGAATGG CCATTAGTCT ATGTGATAAT 
ATTCGACATT TCCTTGTAAG CAAATTGAGG GAAGACATAG ATGATGATAT GTCTGATGAC 
AATGTGGACA CCGAAGAAAA CATTTACAAT TTTTCTACCC CTCGAGTGCA AAGATTTCTT 
CATTATTTAA AGCAAACCTT TAGCAACAAG AATCCAAAGG ATATATGCTG TCTAGTATTT 
GTAGAACGAA GGTACACATG TAAGAGCATC TACGGTATGC TTCTTAAGTA TATCGCCTCA 
GTGCCGGAGT TGAAGAATGT ACTGGTGCCG CAGTTTATGG TGGGACGGAA TGGAATCAAT 
CAAAATTTCG ATACCGTACT GGAACGAAAA TGGCAAAACT CAGCCATTCA ACAATTTCGA 
GATGGCGAGG CAAATCTAAT GATATGCTCA TCCGTATTAG AGGAGGGCAT CGATGTGCAG 
GCCTGTAATT ATGTTATTAT ACTGGATCCA TTGAAGACAT TTAACTCATA TGTGCAAACA 
AAGGGACGGG CTCGGTCTAA AGATGCTGTT TTTCTTATCC TAGCGTCGGA ATCTGAAAAG 
CCAAAGACTG CGAAGAGAAT TATGGACTAT CAGAATGCTC ATAGAGATAT AGCCAATTAT 
CTGCAGGATC GTGTCCTGGA GCGTGCCGAG CCAAAAATGG ATGAAATAAA CGAACATTTT 
AGAGATATAA TTCCGCCTTA TATCAATGAG ACAGGTTCTT TGCTTCTGGC CAGTAGTGCG 
CTAGCATTGC TACATCGTTA TTGTCAAACC TTACCATCCG ATGCATTTGG TTTCAATATG 
CCGTGGTACA AATTGCTTAA CCCAGACGAA CGTGTGCAAC ATTTTGGCAA AAGTGCTGAA 
ACTAAGCAAG TGGTTTCCAT TAATCTGCCA CTTAGCACCA AGCTAAGGAG TACCATATAT 
AGTGATCTTA TGAATTGCAA TAAATCAGCT AAAATTTCGG CTGCCTTCAA TGTGTGTAAG 
GAACTTTATA AACTTGGAGA GCTCAATCAA AAATTTCTGC CAACTACATT AACTGACCGC 
CTAAAAGATG TTTCTGATAT CCATTTTAAG CACTGGAAGA AATACGGCGA CGATGTAACC 
AAAACAAACC GTAAGGAAGA TAAATCAGGC AATACCAGCA AATATCCTGT CAACTGTCCC 
ACCCAATTGT ATGATACACG TCCTCGTGTG GGTGAGACCT GTTATGCTTA TGAGATATTA 
TTGAAGCCAC ATTTCGAGCG CAATGAATAC ACGGCCCATA TGCACGATGC CTTACAGACG 
GGGCGTGGGT ATGCCTTGCT CTTGCGTAAG CGACTACCAC CTCTGGCCGA AATGCCATTG 
TTCAGCAGTC ATGGAAAGAT TCATGTATCG GTCGCTGAGC AGCCGCATGA AGTGATTTTG 
AAAACTGAAG AGCAATTGGA TCATCTGCAT CAATTCCATA TTATGCTGTT TAGGGATATC 
TTGAAAATTT GGCAGTCCTA CTTTGTGCTT GATAAACGCA GCAAGGAGAA TTCCTATCTG 
GTGGTCCCCT TGGATATTAA ATCCAGCGGA AAAGGGGAGG GCAATATAAT AGATTGGAAT 
CTAGTGACGC AGTTCTATCG TGGTCTACCA CAATCAAGAA CTCTAAGCCC GGATCAGAGA 
CGCAGGCAAG AACCACCATG CCCGGATGAC TTTGAGGGTA AAATTGTGAC CCAATGGTAT 
GCAAGTTATC AAGATAAACG GATGTTGGTC ACCAAGGTGC ACAGGGATAA GACCCCATTT 
AGCATGATGG AAAGTAACCA AAGCGAAAAT AATTATTATG ACTACACCAT GTCCAAGTAT 
AAAAATGTTA TCGGAGATGT GGTCCATCAA AATCAGTGCC TTATCGAAGT TCGTGAGTTA 
ACACAGCAAT TAAATTTCTA TATAGCACAT CGAGTAAAAA CGTCGGGTCA GAGCAAAGCT 
CGTGCGCGAA TTATTTTAAT ACCCGAGCTA TGCTTCAATT TTCAATTTCC CGGCGATCTT 
TGGGCAAAAT TACTCTTTCT GCCAAGCATT CTGAATCGTT TGCATTACCT GCTTCATGCA 
GAGGCACTGC GTCAGCGTAT GAATACTTAC TTGGGACTAC ATGTGCTACC ACAAAATGGT 
CCAAATTATC GGCCCAAAGT GCTGGATGTC GATTTGTCGT TGCGCCGCAA TGTGGATTCT 
ACAGGAAATG CAGTTAGCAT GGAAGTTGAG GAACCACGCT CCATGTTGGA TCCATTGCCC 
ACCAAACAAT TAGAGGTATC AATGGATCGT CTTCAGATTA GTGATCTAGA AGCACCCTGG 
CAAAGTTATA TGGAACCAGT AGACTTGAGT CGCAACATGA TGTTGACTCA TCCAGTTGAA 
CTTGCTTATT ACTGTCAATT CACCAGCGGC CAGATTGTCA AATTTGACAA GATAGAGGAA 
GAGGACAAAG AATTCTGGAT GGAGACGCAA TTCAAAATGT CCAAGGGAAA TATTTACGAC 
TATAACACTA CGGCGAACGA ATCTCTACCA GCCCTGCTAC CCAACTCGAA TCCAATAACT 
CCAGTCGAGC AGCTATCGGT GTTGCAGAAA ACCATTTCCA ATGAACACAT CACTCCCGCC 
GAGCAGAGTG AATTTCTAAC AGCGATTACA AGTTCCGGAT GCTCGGATGT CTTTGATATG 
GAGCGTTTGG AATTGTTGGG TGATAGTTTT CTAAAGATGA GTGCAACATT ATATTTGTCA 
AGCATTCATA GCAACTGGAA CGAAGGCGTT CTCACCCAAA TAAAATCCAC ATTGGTGTCC 
AATCGCAATC TTTTATATTG CATGAACGAA ACCGATATTC CTTCGAGAAT CAATAGCACC 
TTGTTCACTC CCAAGTACAC CTGGAAACCG CCTGGCCTTA GTTTGCCACA TAATATATTA 
AATCTATGGC AGGAACAGCC AGAAGTCGCC AAATTTGTAT TACCAAGAAA TCTGTGTGCC 
CTACAACTTA CTGAGGAAGA GATTTTCTCA TTGGGAAAAT TAACCCCGGC AAACTTTGAA 
AAGTTTTTGG AAAGCTGTCA GGCCAACACG AATTACACCA GTACGGATTA TGAATCTGAA 
ATGAATTTCT GTATCGGTAA ATCAAAACTG TCAAACAAAG TGGTCGCCGA CACGTTGGAA 
GCTCTCCTTG GCGTTATTGT TAAGAACTAT GGTGTTCAGC ATGGTTTCCG CATTTTGGAA 
TATTTTGGCA TCTGCAAGCA GGTAGACATT GGCAAGCCAT TGTCCCAACT TCTTGACCTT 
CAATTGGGTA GCACTCGTAC ACGAGCGGAT CGTGATCCTC GTGAGATTGA TGGACTACTC 
ATCAATTATC ATTATTTGGA GCGAAATCTT GGATACACCT TCAAAGATCG CGCCTATTTA 
CTACAGGCTC TCACACATCC ATCGAATCCC ACTAACCGTT TGACTGGCTG CTATCAAGAG 
TTGGAGTTTA TTGGTGATGC CATTTTGGAT TTCCTAATCT CAGCATATAT TTTTGAGCGA 
AATATCCGCA TGAATCCTGG CCAACTGACA GATTTGCGAT CCGCTTTGGT CAATAACACA 
ACATTGGCCT GCATTTGTGT TAGGCATCGG TTCCATTTAT TCATTTTGGC TGAAAATACA 
CTACTTACCG AATCCATCAA TAGTTTTGTG GATTTCCAGG AAAAACATAT GCACCGTGTA 
ACCCATCATG TGCGAATACT TCTTGAAGAA AACGATATAC AATGTCACAA TCCCTTGGAC 
GAGGAAGATG AATTGGAAAT GGCCGACAAT TTGTCTACCA ACGATGAACC GCTAGCCAAG 
GGTAACTTGA ATATGTCGGA AAATGTAGAT GTGCCCAAGG CCTTGGGTGA TGTTGTAGAA 
GCCTTAATTG CAGCCGTGTA TCTTGATTGC AAGGATTTAC AAAAAACCTG GGAAGTGGTA 
TACAGACTCT TTGAGCCTGA ACTTATTGAA TTCTCACGCA ATGTGCCTAT AAATCCTCTT 
CGTCAACTCA ATGAGCATAG GCTAGCTAAA CCGATATTCA GTCCTGCCAT TATGGATGAG 
GGTAAGGTGA TGGTTAGTTG CTTATTCACC TGCAATGAGA AAACCATTAA GGTCTATGGA 
TTTGGTACCA ACAACAGCCA AGCTAAGCTG GCGGCAGCAA AACACGCTCT ACGCAAACTT 
GCCAAGAGCG AAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6637983 ( XM_002060985.3 ) cDNA ORF clone, Drosophila willistoni -> XP_002061021.2 Drosophila willistoni endoribonuclease Dicer (LOC6637983), transcript variant X1, mRNA.

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CloneID	ODf22014
Clone ID Related Accession (Same CDS sequence)	XM_002060985.2 , XM_002060985.3 , XM_023175056.1
Accession Version	XM_002060985.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512230400000
Organism	Drosophila willistoni
Product	endoribonuclease Dicer
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002032340.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 4, 2017 this sequence version replaced XM_002060985.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila willistoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGCTA ACGCGATGAC AACCCCACCC AAGGAAATGC AAGTACGTAG TTATCAATCG 
CGTCTGGTTG ACTATCTAAC TGAGCGCAAT GGGATAGTCT ATTTGCCCAC AGGAGCCGGC 
AAAACCTTTG TGGCCATCCT GACACTAAAA CGTTTCTCAT TGGACTTTGA TAAAACCATC 
GAGGATGGCG GAAAACGAGC TATATTCATG TGCAACACTG TCGAATTAGC CCGTCAACAG 
GCATTGGCTT GTAGATCACT TACAAATTTC AAAGTTGGAT TTTATGTAGG CGATCAAGGT 
GTCGATGAAT GGAGTAGATC CAAATGGGAT GTTGAAATAA GAGAAAAACA AGTTCTTGTT 
GGCACTGCCC AGGTCATGTT GGACCTGATG CTTCACGCCC ATCTGTCTCT GGGCTCCGTA 
AGCATTGTGA TTATAGATGA ATGTCATCAT GGCACCGGAC GTCATCCGTT TCGTGAATTT 
ATGCGTTTAT TTCTACACGT TCGGCCTAAT GTGCAACTGC CACGAGTTGT TGGGCTTACC 
GGAGTCTTGA TCAAGGGCAA TGAGATCAAC AATGTGAAAA AAATGTTGAA AAATCTGGAG 
ACAACATATC ATGGCAATAT TATCACCGTG TCCGATATGA AAGAAATGGA GAATGTCATG 
CTTTACTCGA CCAAGCCCAA GGAGTTTTTT TTGTTATATG GCTATGGAAC TCATAAATTT 
GAGCTCATAG ATACTATTAA ATATTACATT GAAGCGTTTC ACAAGAAGTT GGACAATTAC 
GATATTGGCA AACAACCAGT CCGTTTCTCC AATGGTCTGC AGCAGCAGCG GGAGCCAAAC 
AAGAAAAATT TTGCAAAAAG TCTACTTAAT GATTTTATTT ATCAACTGGA AGAGTATGGT 
ATCTACGTGG CGTCTATAGC AGTCGTTGCT TTACTAGTTG AGTTTGAAAT AAAGAAACGA 
CAGGCCGAGA CGCTGGTCTT AAGATCAATA TACCGAATGG CCATTAGTCT ATGTGATAAT 
ATTCGACATT TCCTTGTAAG CAAATTGAGG GAAGACATAG ATGATGATAT GTCTGATGAC 
AATGTGGACA CCGAAGAAAA CATTTACAAT TTTTCTACCC CTCGAGTGCA AAGATTTCTT 
CATTATTTAA AGCAAACCTT TAGCAACAAG AATCCAAAGG ATATATGCTG TCTAGTATTT 
GTAGAACGAA GGTACACATG TAAGAGCATC TACGGTATGC TTCTTAAGTA TATCGCCTCA 
GTGCCGGAGT TGAAGAATGT ACTGGTGCCG CAGTTTATGG TGGGACGGAA TGGAATCAAT 
CAAAATTTCG ATACCGTACT GGAACGAAAA TGGCAAAACT CAGCCATTCA ACAATTTCGA 
GATGGCGAGG CAAATCTAAT GATATGCTCA TCCGTATTAG AGGAGGGCAT CGATGTGCAG 
GCCTGTAATT ATGTTATTAT ACTGGATCCA TTGAAGACAT TTAACTCATA TGTGCAAACA 
AAGGGACGGG CTCGGTCTAA AGATGCTGTT TTTCTTATCC TAGCGTCGGA ATCTGAAAAG 
CCAAAGACTG CGAAGAGAAT TATGGACTAT CAGAATGCTC ATAGAGATAT AGCCAATTAT 
CTGCAGGATC GTGTCCTGGA GCGTGCCGAG CCAAAAATGG ATGAAATAAA CGAACATTTT 
AGAGATATAA TTCCGCCTTA TATCAATGAG ACAGGTTCTT TGCTTCTGGC CAGTAGTGCG 
CTAGCATTGC TACATCGTTA TTGTCAAACC TTACCATCCG ATGCATTTGG TTTCAATATG 
CCGTGGTACA AATTGCTTAA CCCAGACGAA CGTGTGCAAC ATTTTGGCAA AAGTGCTGAA 
ACTAAGCAAG TGGTTTCCAT TAATCTGCCA CTTAGCACCA AGCTAAGGAG TACCATATAT 
AGTGATCTTA TGAATTGCAA TAAATCAGCT AAAATTTCGG CTGCCTTCAA TGTGTGTAAG 
GAACTTTATA AACTTGGAGA GCTCAATCAA AAATTTCTGC CAACTACATT AACTGACCGC 
CTAAAAGATG TTTCTGATAT CCATTTTAAG CACTGGAAGA AATACGGCGA CGATGTAACC 
AAAACAAACC GTAAGGAAGA TAAATCAGGC AATACCAGCA AATATCCTGT CAACTGTCCC 
ACCCAATTGT ATGATACACG TCCTCGTGTG GGTGAGACCT GTTATGCTTA TGAGATATTA 
TTGAAGCCAC ATTTCGAGCG CAATGAATAC ACGGCCCATA TGCACGATGC CTTACAGACG 
GGGCGTGGGT ATGCCTTGCT CTTGCGTAAG CGACTACCAC CTCTGGCCGA AATGCCATTG 
TTCAGCAGTC ATGGAAAGAT TCATGTATCG GTCGCTGAGC AGCCGCATGA AGTGATTTTG 
AAAACTGAAG AGCAATTGGA TCATCTGCAT CAATTCCATA TTATGCTGTT TAGGGATATC 
TTGAAAATTT GGCAGTCCTA CTTTGTGCTT GATAAACGCA GCAAGGAGAA TTCCTATCTG 
GTGGTCCCCT TGGATATTAA ATCCAGCGGA AAAGGGGAGG GCAATATAAT AGATTGGAAT 
CTAGTGACGC AGTTCTATCG TGGTCTACCA CAATCAAGAA CTCTAAGCCC GGATCAGAGA 
CGCAGGCAAG AACCACCATG CCCGGATGAC TTTGAGGGTA AAATTGTGAC CCAATGGTAT 
GCAAGTTATC AAGATAAACG GATGTTGGTC ACCAAGGTGC ACAGGGATAA GACCCCATTT 
AGCATGATGG AAAGTAACCA AAGCGAAAAT AATTATTATG ACTACACCAT GTCCAAGTAT 
AAAAATGTTA TCGGAGATGT GGTCCATCAA AATCAGTGCC TTATCGAAGT TCGTGAGTTA 
ACACAGCAAT TAAATTTCTA TATAGCACAT CGAGTAAAAA CGTCGGGTCA GAGCAAAGCT 
CGTGCGCGAA TTATTTTAAT ACCCGAGCTA TGCTTCAATT TTCAATTTCC CGGCGATCTT 
TGGGCAAAAT TACTCTTTCT GCCAAGCATT CTGAATCGTT TGCATTACCT GCTTCATGCA 
GAGGCACTGC GTCAGCGTAT GAATACTTAC TTGGGACTAC ATGTGCTACC ACAAAATGGT 
CCAAATTATC GGCCCAAAGT GCTGGATGTC GATTTGTCGT TGCGCCGCAA TGTGGATTCT 
ACAGGAAATG CAGTTAGCAT GGAAGTTGAG GAACCACGCT CCATGTTGGA TCCATTGCCC 
ACCAAACAAT TAGAGGTATC AATGGATCGT CTTCAGATTA GTGATCTAGA AGCACCCTGG 
CAAAGTTATA TGGAACCAGT AGACTTGAGT CGCAACATGA TGTTGACTCA TCCAGTTGAA 
CTTGCTTATT ACTGTCAATT CACCAGCGGC CAGATTGTCA AATTTGACAA GATAGAGGAA 
GAGGACAAAG AATTCTGGAT GGAGACGCAA TTCAAAATGT CCAAGGGAAA TATTTACGAC 
TATAACACTA CGGCGAACGA ATCTCTACCA GCCCTGCTAC CCAACTCGAA TCCAATAACT 
CCAGTCGAGC AGCTATCGGT GTTGCAGAAA ACCATTTCCA ATGAACACAT CACTCCCGCC 
GAGCAGAGTG AATTTCTAAC AGCGATTACA AGTTCCGGAT GCTCGGATGT CTTTGATATG 
GAGCGTTTGG AATTGTTGGG TGATAGTTTT CTAAAGATGA GTGCAACATT ATATTTGTCA 
AGCATTCATA GCAACTGGAA CGAAGGCGTT CTCACCCAAA TAAAATCCAC ATTGGTGTCC 
AATCGCAATC TTTTATATTG CATGAACGAA ACCGATATTC CTTCGAGAAT CAATAGCACC 
TTGTTCACTC CCAAGTACAC CTGGAAACCG CCTGGCCTTA GTTTGCCACA TAATATATTA 
AATCTATGGC AGGAACAGCC AGAAGTCGCC AAATTTGTAT TACCAAGAAA TCTGTGTGCC 
CTACAACTTA CTGAGGAAGA GATTTTCTCA TTGGGAAAAT TAACCCCGGC AAACTTTGAA 
AAGTTTTTGG AAAGCTGTCA GGCCAACACG AATTACACCA GTACGGATTA TGAATCTGAA 
ATGAATTTCT GTATCGGTAA ATCAAAACTG TCAAACAAAG TGGTCGCCGA CACGTTGGAA 
GCTCTCCTTG GCGTTATTGT TAAGAACTAT GGTGTTCAGC ATGGTTTCCG CATTTTGGAA 
TATTTTGGCA TCTGCAAGCA GGTAGACATT GGCAAGCCAT TGTCCCAACT TCTTGACCTT 
CAATTGGGTA GCACTCGTAC ACGAGCGGAT CGTGATCCTC GTGAGATTGA TGGACTACTC 
ATCAATTATC ATTATTTGGA GCGAAATCTT GGATACACCT TCAAAGATCG CGCCTATTTA 
CTACAGGCTC TCACACATCC ATCGAATCCC ACTAACCGTT TGACTGGCTG CTATCAAGAG 
TTGGAGTTTA TTGGTGATGC CATTTTGGAT TTCCTAATCT CAGCATATAT TTTTGAGCGA 
AATATCCGCA TGAATCCTGG CCAACTGACA GATTTGCGAT CCGCTTTGGT CAATAACACA 
ACATTGGCCT GCATTTGTGT TAGGCATCGG TTCCATTTAT TCATTTTGGC TGAAAATACA 
CTACTTACCG AATCCATCAA TAGTTTTGTG GATTTCCAGG AAAAACATAT GCACCGTGTA 
ACCCATCATG TGCGAATACT TCTTGAAGAA AACGATATAC AATGTCACAA TCCCTTGGAC 
GAGGAAGATG AATTGGAAAT GGCCGACAAT TTGTCTACCA ACGATGAACC GCTAGCCAAG 
GGTAACTTGA ATATGTCGGA AAATGTAGAT GTGCCCAAGG CCTTGGGTGA TGTTGTAGAA 
GCCTTAATTG CAGCCGTGTA TCTTGATTGC AAGGATTTAC AAAAAACCTG GGAAGTGGTA 
TACAGACTCT TTGAGCCTGA ACTTATTGAA TTCTCACGCA ATGTGCCTAT AAATCCTCTT 
CGTCAACTCA ATGAGCATAG GCTAGCTAAA CCGATATTCA GTCCTGCCAT TATGGATGAG 
GGTAAGGTGA TGGTTAGTTG CTTATTCACC TGCAATGAGA AAACCATTAA GGTCTATGGA 
TTTGGTACCA ACAACAGCCA AGCTAAGCTG GCGGCAGCAA AACACGCTCT ACGCAAACTT 
GCCAAGAGCG AAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002061021.2
CDS	185..5362
Translation	MPANAMTTPPKEMQVRSYQSRLVDYLTERNGIVYLPTGAGKTFVAILTLKRFSLDFDKTIEDGGKRAIFMCNTVELARQQALACRSLTNFKVGFYVGDQGVDEWSRSKWDVEIREKQVLVGTAQVMLDLMLHAHLSLGSVSIVIIDECHHGTGRHPFREFMRLFLHVRPNVQLPRVVGLTGVLIKGNEINNVKKMLKNLETTYHGNIITVSDMKEMENVMLYSTKPKEFFLLYGYGTHKFELIDTIKYYIEAFHKKLDNYDIGKQPVRFSNGLQQQREPNKKNFAKSLLNDFIYQLEEYGIYVASIAVVALLVEFEIKKRQAETLVLRSIYRMAISLCDNIRHFLVSKLREDIDDDMSDDNVDTEENIYNFSTPRVQRFLHYLKQTFSNKNPKDICCLVFVERRYTCKSIYGMLLKYIASVPELKNVLVPQFMVGRNGINQNFDTVLERKWQNSAIQQFRDGEANLMICSSVLEEGIDVQACNYVIILDPLKTFNSYVQTKGRARSKDAVFLILASESEKPKTAKRIMDYQNAHRDIANYLQDRVLERAEPKMDEINEHFRDIIPPYINETGSLLLASSALALLHRYCQTLPSDAFGFNMPWYKLLNPDERVQHFGKSAETKQVVSINLPLSTKLRSTIYSDLMNCNKSAKISAAFNVCKELYKLGELNQKFLPTTLTDRLKDVSDIHFKHWKKYGDDVTKTNRKEDKSGNTSKYPVNCPTQLYDTRPRVGETCYAYEILLKPHFERNEYTAHMHDALQTGRGYALLLRKRLPPLAEMPLFSSHGKIHVSVAEQPHEVILKTEEQLDHLHQFHIMLFRDILKIWQSYFVLDKRSKENSYLVVPLDIKSSGKGEGNIIDWNLVTQFYRGLPQSRTLSPDQRRRQEPPCPDDFEGKIVTQWYASYQDKRMLVTKVHRDKTPFSMMESNQSENNYYDYTMSKYKNVIGDVVHQNQCLIEVRELTQQLNFYIAHRVKTSGQSKARARIILIPELCFNFQFPGDLWAKLLFLPSILNRLHYLLHAEALRQRMNTYLGLHVLPQNGPNYRPKVLDVDLSLRRNVDSTGNAVSMEVEEPRSMLDPLPTKQLEVSMDRLQISDLEAPWQSYMEPVDLSRNMMLTHPVELAYYCQFTSGQIVKFDKIEEEDKEFWMETQFKMSKGNIYDYNTTANESLPALLPNSNPITPVEQLSVLQKTISNEHITPAEQSEFLTAITSSGCSDVFDMERLELLGDSFLKMSATLYLSSIHSNWNEGVLTQIKSTLVSNRNLLYCMNETDIPSRINSTLFTPKYTWKPPGLSLPHNILNLWQEQPEVAKFVLPRNLCALQLTEEEIFSLGKLTPANFEKFLESCQANTNYTSTDYESEMNFCIGKSKLSNKVVADTLEALLGVIVKNYGVQHGFRILEYFGICKQVDIGKPLSQLLDLQLGSTRTRADRDPREIDGLLINYHYLERNLGYTFKDRAYLLQALTHPSNPTNRLTGCYQELEFIGDAILDFLISAYIFERNIRMNPGQLTDLRSALVNNTTLACICVRHRFHLFILAENTLLTESINSFVDFQEKHMHRVTHHVRILLEENDIQCHNPLDEEDELEMADNLSTNDEPLAKGNLNMSENVDVPKALGDVVEALIAAVYLDCKDLQKTWEVVYRLFEPELIEFSRNVPINPLRQLNEHRLAKPIFSPAIMDEGKVMVSCLFTCNEKTIKVYGFGTNNSQAKLAAAKHALRKLAKSEA

Target ORF information:

RefSeq Version	XM_002060985.3
Organism	Drosophila willistoni
Definition	Drosophila willistoni endoribonuclease Dicer (LOC6637983), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002060985.3

ORF Insert Sequence:

ATGCCTGCTA ACGCGATGAC AACCCCACCC AAGGAAATGC AAGTACGTAG TTATCAATCG 
CGTCTGGTTG ACTATCTAAC TGAGCGCAAT GGGATAGTCT ATTTGCCCAC AGGAGCCGGC 
AAAACCTTTG TGGCCATCCT GACACTAAAA CGTTTCTCAT TGGACTTTGA TAAAACCATC 
GAGGATGGCG GAAAACGAGC TATATTCATG TGCAACACTG TCGAATTAGC CCGTCAACAG 
GCATTGGCTT GTAGATCACT TACAAATTTC AAAGTTGGAT TTTATGTAGG CGATCAAGGT 
GTCGATGAAT GGAGTAGATC CAAATGGGAT GTTGAAATAA GAGAAAAACA AGTTCTTGTT 
GGCACTGCCC AGGTCATGTT GGACCTGATG CTTCACGCCC ATCTGTCTCT GGGCTCCGTA 
AGCATTGTGA TTATAGATGA ATGTCATCAT GGCACCGGAC GTCATCCGTT TCGTGAATTT 
ATGCGTTTAT TTCTACACGT TCGGCCTAAT GTGCAACTGC CACGAGTTGT TGGGCTTACC 
GGAGTCTTGA TCAAGGGCAA TGAGATCAAC AATGTGAAAA AAATGTTGAA AAATCTGGAG 
ACAACATATC ATGGCAATAT TATCACCGTG TCCGATATGA AAGAAATGGA GAATGTCATG 
CTTTACTCGA CCAAGCCCAA GGAGTTTTTT TTGTTATATG GCTATGGAAC TCATAAATTT 
GAGCTCATAG ATACTATTAA ATATTACATT GAAGCGTTTC ACAAGAAGTT GGACAATTAC 
GATATTGGCA AACAACCAGT CCGTTTCTCC AATGGTCTGC AGCAGCAGCG GGAGCCAAAC 
AAGAAAAATT TTGCAAAAAG TCTACTTAAT GATTTTATTT ATCAACTGGA AGAGTATGGT 
ATCTACGTGG CGTCTATAGC AGTCGTTGCT TTACTAGTTG AGTTTGAAAT AAAGAAACGA 
CAGGCCGAGA CGCTGGTCTT AAGATCAATA TACCGAATGG CCATTAGTCT ATGTGATAAT 
ATTCGACATT TCCTTGTAAG CAAATTGAGG GAAGACATAG ATGATGATAT GTCTGATGAC 
AATGTGGACA CCGAAGAAAA CATTTACAAT TTTTCTACCC CTCGAGTGCA AAGATTTCTT 
CATTATTTAA AGCAAACCTT TAGCAACAAG AATCCAAAGG ATATATGCTG TCTAGTATTT 
GTAGAACGAA GGTACACATG TAAGAGCATC TACGGTATGC TTCTTAAGTA TATCGCCTCA 
GTGCCGGAGT TGAAGAATGT ACTGGTGCCG CAGTTTATGG TGGGACGGAA TGGAATCAAT 
CAAAATTTCG ATACCGTACT GGAACGAAAA TGGCAAAACT CAGCCATTCA ACAATTTCGA 
GATGGCGAGG CAAATCTAAT GATATGCTCA TCCGTATTAG AGGAGGGCAT CGATGTGCAG 
GCCTGTAATT ATGTTATTAT ACTGGATCCA TTGAAGACAT TTAACTCATA TGTGCAAACA 
AAGGGACGGG CTCGGTCTAA AGATGCTGTT TTTCTTATCC TAGCGTCGGA ATCTGAAAAG 
CCAAAGACTG CGAAGAGAAT TATGGACTAT CAGAATGCTC ATAGAGATAT AGCCAATTAT 
CTGCAGGATC GTGTCCTGGA GCGTGCCGAG CCAAAAATGG ATGAAATAAA CGAACATTTT 
AGAGATATAA TTCCGCCTTA TATCAATGAG ACAGGTTCTT TGCTTCTGGC CAGTAGTGCG 
CTAGCATTGC TACATCGTTA TTGTCAAACC TTACCATCCG ATGCATTTGG TTTCAATATG 
CCGTGGTACA AATTGCTTAA CCCAGACGAA CGTGTGCAAC ATTTTGGCAA AAGTGCTGAA 
ACTAAGCAAG TGGTTTCCAT TAATCTGCCA CTTAGCACCA AGCTAAGGAG TACCATATAT 
AGTGATCTTA TGAATTGCAA TAAATCAGCT AAAATTTCGG CTGCCTTCAA TGTGTGTAAG 
GAACTTTATA AACTTGGAGA GCTCAATCAA AAATTTCTGC CAACTACATT AACTGACCGC 
CTAAAAGATG TTTCTGATAT CCATTTTAAG CACTGGAAGA AATACGGCGA CGATGTAACC 
AAAACAAACC GTAAGGAAGA TAAATCAGGC AATACCAGCA AATATCCTGT CAACTGTCCC 
ACCCAATTGT ATGATACACG TCCTCGTGTG GGTGAGACCT GTTATGCTTA TGAGATATTA 
TTGAAGCCAC ATTTCGAGCG CAATGAATAC ACGGCCCATA TGCACGATGC CTTACAGACG 
GGGCGTGGGT ATGCCTTGCT CTTGCGTAAG CGACTACCAC CTCTGGCCGA AATGCCATTG 
TTCAGCAGTC ATGGAAAGAT TCATGTATCG GTCGCTGAGC AGCCGCATGA AGTGATTTTG 
AAAACTGAAG AGCAATTGGA TCATCTGCAT CAATTCCATA TTATGCTGTT TAGGGATATC 
TTGAAAATTT GGCAGTCCTA CTTTGTGCTT GATAAACGCA GCAAGGAGAA TTCCTATCTG 
GTGGTCCCCT TGGATATTAA ATCCAGCGGA AAAGGGGAGG GCAATATAAT AGATTGGAAT 
CTAGTGACGC AGTTCTATCG TGGTCTACCA CAATCAAGAA CTCTAAGCCC GGATCAGAGA 
CGCAGGCAAG AACCACCATG CCCGGATGAC TTTGAGGGTA AAATTGTGAC CCAATGGTAT 
GCAAGTTATC AAGATAAACG GATGTTGGTC ACCAAGGTGC ACAGGGATAA GACCCCATTT 
AGCATGATGG AAAGTAACCA AAGCGAAAAT AATTATTATG ACTACACCAT GTCCAAGTAT 
AAAAATGTTA TCGGAGATGT GGTCCATCAA AATCAGTGCC TTATCGAAGT TCGTGAGTTA 
ACACAGCAAT TAAATTTCTA TATAGCACAT CGAGTAAAAA CGTCGGGTCA GAGCAAAGCT 
CGTGCGCGAA TTATTTTAAT ACCCGAGCTA TGCTTCAATT TTCAATTTCC CGGCGATCTT 
TGGGCAAAAT TACTCTTTCT GCCAAGCATT CTGAATCGTT TGCATTACCT GCTTCATGCA 
GAGGCACTGC GTCAGCGTAT GAATACTTAC TTGGGACTAC ATGTGCTACC ACAAAATGGT 
CCAAATTATC GGCCCAAAGT GCTGGATGTC GATTTGTCGT TGCGCCGCAA TGTGGATTCT 
ACAGGAAATG CAGTTAGCAT GGAAGTTGAG GAACCACGCT CCATGTTGGA TCCATTGCCC 
ACCAAACAAT TAGAGGTATC AATGGATCGT CTTCAGATTA GTGATCTAGA AGCACCCTGG 
CAAAGTTATA TGGAACCAGT AGACTTGAGT CGCAACATGA TGTTGACTCA TCCAGTTGAA 
CTTGCTTATT ACTGTCAATT CACCAGCGGC CAGATTGTCA AATTTGACAA GATAGAGGAA 
GAGGACAAAG AATTCTGGAT GGAGACGCAA TTCAAAATGT CCAAGGGAAA TATTTACGAC 
TATAACACTA CGGCGAACGA ATCTCTACCA GCCCTGCTAC CCAACTCGAA TCCAATAACT 
CCAGTCGAGC AGCTATCGGT GTTGCAGAAA ACCATTTCCA ATGAACACAT CACTCCCGCC 
GAGCAGAGTG AATTTCTAAC AGCGATTACA AGTTCCGGAT GCTCGGATGT CTTTGATATG 
GAGCGTTTGG AATTGTTGGG TGATAGTTTT CTAAAGATGA GTGCAACATT ATATTTGTCA 
AGCATTCATA GCAACTGGAA CGAAGGCGTT CTCACCCAAA TAAAATCCAC ATTGGTGTCC 
AATCGCAATC TTTTATATTG CATGAACGAA ACCGATATTC CTTCGAGAAT CAATAGCACC 
TTGTTCACTC CCAAGTACAC CTGGAAACCG CCTGGCCTTA GTTTGCCACA TAATATATTA 
AATCTATGGC AGGAACAGCC AGAAGTCGCC AAATTTGTAT TACCAAGAAA TCTGTGTGCC 
CTACAACTTA CTGAGGAAGA GATTTTCTCA TTGGGAAAAT TAACCCCGGC AAACTTTGAA 
AAGTTTTTGG AAAGCTGTCA GGCCAACACG AATTACACCA GTACGGATTA TGAATCTGAA 
ATGAATTTCT GTATCGGTAA ATCAAAACTG TCAAACAAAG TGGTCGCCGA CACGTTGGAA 
GCTCTCCTTG GCGTTATTGT TAAGAACTAT GGTGTTCAGC ATGGTTTCCG CATTTTGGAA 
TATTTTGGCA TCTGCAAGCA GGTAGACATT GGCAAGCCAT TGTCCCAACT TCTTGACCTT 
CAATTGGGTA GCACTCGTAC ACGAGCGGAT CGTGATCCTC GTGAGATTGA TGGACTACTC 
ATCAATTATC ATTATTTGGA GCGAAATCTT GGATACACCT TCAAAGATCG CGCCTATTTA 
CTACAGGCTC TCACACATCC ATCGAATCCC ACTAACCGTT TGACTGGCTG CTATCAAGAG 
TTGGAGTTTA TTGGTGATGC CATTTTGGAT TTCCTAATCT CAGCATATAT TTTTGAGCGA 
AATATCCGCA TGAATCCTGG CCAACTGACA GATTTGCGAT CCGCTTTGGT CAATAACACA 
ACATTGGCCT GCATTTGTGT TAGGCATCGG TTCCATTTAT TCATTTTGGC TGAAAATACA 
CTACTTACCG AATCCATCAA TAGTTTTGTG GATTTCCAGG AAAAACATAT GCACCGTGTA 
ACCCATCATG TGCGAATACT TCTTGAAGAA AACGATATAC AATGTCACAA TCCCTTGGAC 
GAGGAAGATG AATTGGAAAT GGCCGACAAT TTGTCTACCA ACGATGAACC GCTAGCCAAG 
GGTAACTTGA ATATGTCGGA AAATGTAGAT GTGCCCAAGG CCTTGGGTGA TGTTGTAGAA 
GCCTTAATTG CAGCCGTGTA TCTTGATTGC AAGGATTTAC AAAAAACCTG GGAAGTGGTA 
TACAGACTCT TTGAGCCTGA ACTTATTGAA TTCTCACGCA ATGTGCCTAT AAATCCTCTT 
CGTCAACTCA ATGAGCATAG GCTAGCTAAA CCGATATTCA GTCCTGCCAT TATGGATGAG 
GGTAAGGTGA TGGTTAGTTG CTTATTCACC TGCAATGAGA AAACCATTAA GGTCTATGGA 
TTTGGTACCA ACAACAGCCA AGCTAAGCTG GCGGCAGCAA AACACGCTCT ACGCAAACTT 
GCCAAGAGCG AAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6637983 ( XM_023175056.1 ) cDNA ORF clone, Drosophila willistoni -> XP_023030824.1 Drosophila willistoni endoribonuclease Dicer (LOC6637983), transcript variant X2, mRNA.

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CloneID	ODf22014
Clone ID Related Accession (Same CDS sequence)	XM_002060985.2 , XM_002060985.3 , XM_023175056.1
Accession Version	XM_023175056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512230400000
Organism	Drosophila willistoni
Product	endoribonuclease Dicer
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002032340.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila willistoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGCTA ACGCGATGAC AACCCCACCC AAGGAAATGC AAGTACGTAG TTATCAATCG 
CGTCTGGTTG ACTATCTAAC TGAGCGCAAT GGGATAGTCT ATTTGCCCAC AGGAGCCGGC 
AAAACCTTTG TGGCCATCCT GACACTAAAA CGTTTCTCAT TGGACTTTGA TAAAACCATC 
GAGGATGGCG GAAAACGAGC TATATTCATG TGCAACACTG TCGAATTAGC CCGTCAACAG 
GCATTGGCTT GTAGATCACT TACAAATTTC AAAGTTGGAT TTTATGTAGG CGATCAAGGT 
GTCGATGAAT GGAGTAGATC CAAATGGGAT GTTGAAATAA GAGAAAAACA AGTTCTTGTT 
GGCACTGCCC AGGTCATGTT GGACCTGATG CTTCACGCCC ATCTGTCTCT GGGCTCCGTA 
AGCATTGTGA TTATAGATGA ATGTCATCAT GGCACCGGAC GTCATCCGTT TCGTGAATTT 
ATGCGTTTAT TTCTACACGT TCGGCCTAAT GTGCAACTGC CACGAGTTGT TGGGCTTACC 
GGAGTCTTGA TCAAGGGCAA TGAGATCAAC AATGTGAAAA AAATGTTGAA AAATCTGGAG 
ACAACATATC ATGGCAATAT TATCACCGTG TCCGATATGA AAGAAATGGA GAATGTCATG 
CTTTACTCGA CCAAGCCCAA GGAGTTTTTT TTGTTATATG GCTATGGAAC TCATAAATTT 
GAGCTCATAG ATACTATTAA ATATTACATT GAAGCGTTTC ACAAGAAGTT GGACAATTAC 
GATATTGGCA AACAACCAGT CCGTTTCTCC AATGGTCTGC AGCAGCAGCG GGAGCCAAAC 
AAGAAAAATT TTGCAAAAAG TCTACTTAAT GATTTTATTT ATCAACTGGA AGAGTATGGT 
ATCTACGTGG CGTCTATAGC AGTCGTTGCT TTACTAGTTG AGTTTGAAAT AAAGAAACGA 
CAGGCCGAGA CGCTGGTCTT AAGATCAATA TACCGAATGG CCATTAGTCT ATGTGATAAT 
ATTCGACATT TCCTTGTAAG CAAATTGAGG GAAGACATAG ATGATGATAT GTCTGATGAC 
AATGTGGACA CCGAAGAAAA CATTTACAAT TTTTCTACCC CTCGAGTGCA AAGATTTCTT 
CATTATTTAA AGCAAACCTT TAGCAACAAG AATCCAAAGG ATATATGCTG TCTAGTATTT 
GTAGAACGAA GGTACACATG TAAGAGCATC TACGGTATGC TTCTTAAGTA TATCGCCTCA 
GTGCCGGAGT TGAAGAATGT ACTGGTGCCG CAGTTTATGG TGGGACGGAA TGGAATCAAT 
CAAAATTTCG ATACCGTACT GGAACGAAAA TGGCAAAACT CAGCCATTCA ACAATTTCGA 
GATGGCGAGG CAAATCTAAT GATATGCTCA TCCGTATTAG AGGAGGGCAT CGATGTGCAG 
GCCTGTAATT ATGTTATTAT ACTGGATCCA TTGAAGACAT TTAACTCATA TGTGCAAACA 
AAGGGACGGG CTCGGTCTAA AGATGCTGTT TTTCTTATCC TAGCGTCGGA ATCTGAAAAG 
CCAAAGACTG CGAAGAGAAT TATGGACTAT CAGAATGCTC ATAGAGATAT AGCCAATTAT 
CTGCAGGATC GTGTCCTGGA GCGTGCCGAG CCAAAAATGG ATGAAATAAA CGAACATTTT 
AGAGATATAA TTCCGCCTTA TATCAATGAG ACAGGTTCTT TGCTTCTGGC CAGTAGTGCG 
CTAGCATTGC TACATCGTTA TTGTCAAACC TTACCATCCG ATGCATTTGG TTTCAATATG 
CCGTGGTACA AATTGCTTAA CCCAGACGAA CGTGTGCAAC ATTTTGGCAA AAGTGCTGAA 
ACTAAGCAAG TGGTTTCCAT TAATCTGCCA CTTAGCACCA AGCTAAGGAG TACCATATAT 
AGTGATCTTA TGAATTGCAA TAAATCAGCT AAAATTTCGG CTGCCTTCAA TGTGTGTAAG 
GAACTTTATA AACTTGGAGA GCTCAATCAA AAATTTCTGC CAACTACATT AACTGACCGC 
CTAAAAGATG TTTCTGATAT CCATTTTAAG CACTGGAAGA AATACGGCGA CGATGTAACC 
AAAACAAACC GTAAGGAAGA TAAATCAGGC AATACCAGCA AATATCCTGT CAACTGTCCC 
ACCCAATTGT ATGATACACG TCCTCGTGTG GGTGAGACCT GTTATGCTTA TGAGATATTA 
TTGAAGCCAC ATTTCGAGCG CAATGAATAC ACGGCCCATA TGCACGATGC CTTACAGACG 
GGGCGTGGGT ATGCCTTGCT CTTGCGTAAG CGACTACCAC CTCTGGCCGA AATGCCATTG 
TTCAGCAGTC ATGGAAAGAT TCATGTATCG GTCGCTGAGC AGCCGCATGA AGTGATTTTG 
AAAACTGAAG AGCAATTGGA TCATCTGCAT CAATTCCATA TTATGCTGTT TAGGGATATC 
TTGAAAATTT GGCAGTCCTA CTTTGTGCTT GATAAACGCA GCAAGGAGAA TTCCTATCTG 
GTGGTCCCCT TGGATATTAA ATCCAGCGGA AAAGGGGAGG GCAATATAAT AGATTGGAAT 
CTAGTGACGC AGTTCTATCG TGGTCTACCA CAATCAAGAA CTCTAAGCCC GGATCAGAGA 
CGCAGGCAAG AACCACCATG CCCGGATGAC TTTGAGGGTA AAATTGTGAC CCAATGGTAT 
GCAAGTTATC AAGATAAACG GATGTTGGTC ACCAAGGTGC ACAGGGATAA GACCCCATTT 
AGCATGATGG AAAGTAACCA AAGCGAAAAT AATTATTATG ACTACACCAT GTCCAAGTAT 
AAAAATGTTA TCGGAGATGT GGTCCATCAA AATCAGTGCC TTATCGAAGT TCGTGAGTTA 
ACACAGCAAT TAAATTTCTA TATAGCACAT CGAGTAAAAA CGTCGGGTCA GAGCAAAGCT 
CGTGCGCGAA TTATTTTAAT ACCCGAGCTA TGCTTCAATT TTCAATTTCC CGGCGATCTT 
TGGGCAAAAT TACTCTTTCT GCCAAGCATT CTGAATCGTT TGCATTACCT GCTTCATGCA 
GAGGCACTGC GTCAGCGTAT GAATACTTAC TTGGGACTAC ATGTGCTACC ACAAAATGGT 
CCAAATTATC GGCCCAAAGT GCTGGATGTC GATTTGTCGT TGCGCCGCAA TGTGGATTCT 
ACAGGAAATG CAGTTAGCAT GGAAGTTGAG GAACCACGCT CCATGTTGGA TCCATTGCCC 
ACCAAACAAT TAGAGGTATC AATGGATCGT CTTCAGATTA GTGATCTAGA AGCACCCTGG 
CAAAGTTATA TGGAACCAGT AGACTTGAGT CGCAACATGA TGTTGACTCA TCCAGTTGAA 
CTTGCTTATT ACTGTCAATT CACCAGCGGC CAGATTGTCA AATTTGACAA GATAGAGGAA 
GAGGACAAAG AATTCTGGAT GGAGACGCAA TTCAAAATGT CCAAGGGAAA TATTTACGAC 
TATAACACTA CGGCGAACGA ATCTCTACCA GCCCTGCTAC CCAACTCGAA TCCAATAACT 
CCAGTCGAGC AGCTATCGGT GTTGCAGAAA ACCATTTCCA ATGAACACAT CACTCCCGCC 
GAGCAGAGTG AATTTCTAAC AGCGATTACA AGTTCCGGAT GCTCGGATGT CTTTGATATG 
GAGCGTTTGG AATTGTTGGG TGATAGTTTT CTAAAGATGA GTGCAACATT ATATTTGTCA 
AGCATTCATA GCAACTGGAA CGAAGGCGTT CTCACCCAAA TAAAATCCAC ATTGGTGTCC 
AATCGCAATC TTTTATATTG CATGAACGAA ACCGATATTC CTTCGAGAAT CAATAGCACC 
TTGTTCACTC CCAAGTACAC CTGGAAACCG CCTGGCCTTA GTTTGCCACA TAATATATTA 
AATCTATGGC AGGAACAGCC AGAAGTCGCC AAATTTGTAT TACCAAGAAA TCTGTGTGCC 
CTACAACTTA CTGAGGAAGA GATTTTCTCA TTGGGAAAAT TAACCCCGGC AAACTTTGAA 
AAGTTTTTGG AAAGCTGTCA GGCCAACACG AATTACACCA GTACGGATTA TGAATCTGAA 
ATGAATTTCT GTATCGGTAA ATCAAAACTG TCAAACAAAG TGGTCGCCGA CACGTTGGAA 
GCTCTCCTTG GCGTTATTGT TAAGAACTAT GGTGTTCAGC ATGGTTTCCG CATTTTGGAA 
TATTTTGGCA TCTGCAAGCA GGTAGACATT GGCAAGCCAT TGTCCCAACT TCTTGACCTT 
CAATTGGGTA GCACTCGTAC ACGAGCGGAT CGTGATCCTC GTGAGATTGA TGGACTACTC 
ATCAATTATC ATTATTTGGA GCGAAATCTT GGATACACCT TCAAAGATCG CGCCTATTTA 
CTACAGGCTC TCACACATCC ATCGAATCCC ACTAACCGTT TGACTGGCTG CTATCAAGAG 
TTGGAGTTTA TTGGTGATGC CATTTTGGAT TTCCTAATCT CAGCATATAT TTTTGAGCGA 
AATATCCGCA TGAATCCTGG CCAACTGACA GATTTGCGAT CCGCTTTGGT CAATAACACA 
ACATTGGCCT GCATTTGTGT TAGGCATCGG TTCCATTTAT TCATTTTGGC TGAAAATACA 
CTACTTACCG AATCCATCAA TAGTTTTGTG GATTTCCAGG AAAAACATAT GCACCGTGTA 
ACCCATCATG TGCGAATACT TCTTGAAGAA AACGATATAC AATGTCACAA TCCCTTGGAC 
GAGGAAGATG AATTGGAAAT GGCCGACAAT TTGTCTACCA ACGATGAACC GCTAGCCAAG 
GGTAACTTGA ATATGTCGGA AAATGTAGAT GTGCCCAAGG CCTTGGGTGA TGTTGTAGAA 
GCCTTAATTG CAGCCGTGTA TCTTGATTGC AAGGATTTAC AAAAAACCTG GGAAGTGGTA 
TACAGACTCT TTGAGCCTGA ACTTATTGAA TTCTCACGCA ATGTGCCTAT AAATCCTCTT 
CGTCAACTCA ATGAGCATAG GCTAGCTAAA CCGATATTCA GTCCTGCCAT TATGGATGAG 
GGTAAGGTGA TGGTTAGTTG CTTATTCACC TGCAATGAGA AAACCATTAA GGTCTATGGA 
TTTGGTACCA ACAACAGCCA AGCTAAGCTG GCGGCAGCAA AACACGCTCT ACGCAAACTT 
GCCAAGAGCG AAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023030824.1
CDS	189..5366
Translation	MPANAMTTPPKEMQVRSYQSRLVDYLTERNGIVYLPTGAGKTFVAILTLKRFSLDFDKTIEDGGKRAIFMCNTVELARQQALACRSLTNFKVGFYVGDQGVDEWSRSKWDVEIREKQVLVGTAQVMLDLMLHAHLSLGSVSIVIIDECHHGTGRHPFREFMRLFLHVRPNVQLPRVVGLTGVLIKGNEINNVKKMLKNLETTYHGNIITVSDMKEMENVMLYSTKPKEFFLLYGYGTHKFELIDTIKYYIEAFHKKLDNYDIGKQPVRFSNGLQQQREPNKKNFAKSLLNDFIYQLEEYGIYVASIAVVALLVEFEIKKRQAETLVLRSIYRMAISLCDNIRHFLVSKLREDIDDDMSDDNVDTEENIYNFSTPRVQRFLHYLKQTFSNKNPKDICCLVFVERRYTCKSIYGMLLKYIASVPELKNVLVPQFMVGRNGINQNFDTVLERKWQNSAIQQFRDGEANLMICSSVLEEGIDVQACNYVIILDPLKTFNSYVQTKGRARSKDAVFLILASESEKPKTAKRIMDYQNAHRDIANYLQDRVLERAEPKMDEINEHFRDIIPPYINETGSLLLASSALALLHRYCQTLPSDAFGFNMPWYKLLNPDERVQHFGKSAETKQVVSINLPLSTKLRSTIYSDLMNCNKSAKISAAFNVCKELYKLGELNQKFLPTTLTDRLKDVSDIHFKHWKKYGDDVTKTNRKEDKSGNTSKYPVNCPTQLYDTRPRVGETCYAYEILLKPHFERNEYTAHMHDALQTGRGYALLLRKRLPPLAEMPLFSSHGKIHVSVAEQPHEVILKTEEQLDHLHQFHIMLFRDILKIWQSYFVLDKRSKENSYLVVPLDIKSSGKGEGNIIDWNLVTQFYRGLPQSRTLSPDQRRRQEPPCPDDFEGKIVTQWYASYQDKRMLVTKVHRDKTPFSMMESNQSENNYYDYTMSKYKNVIGDVVHQNQCLIEVRELTQQLNFYIAHRVKTSGQSKARARIILIPELCFNFQFPGDLWAKLLFLPSILNRLHYLLHAEALRQRMNTYLGLHVLPQNGPNYRPKVLDVDLSLRRNVDSTGNAVSMEVEEPRSMLDPLPTKQLEVSMDRLQISDLEAPWQSYMEPVDLSRNMMLTHPVELAYYCQFTSGQIVKFDKIEEEDKEFWMETQFKMSKGNIYDYNTTANESLPALLPNSNPITPVEQLSVLQKTISNEHITPAEQSEFLTAITSSGCSDVFDMERLELLGDSFLKMSATLYLSSIHSNWNEGVLTQIKSTLVSNRNLLYCMNETDIPSRINSTLFTPKYTWKPPGLSLPHNILNLWQEQPEVAKFVLPRNLCALQLTEEEIFSLGKLTPANFEKFLESCQANTNYTSTDYESEMNFCIGKSKLSNKVVADTLEALLGVIVKNYGVQHGFRILEYFGICKQVDIGKPLSQLLDLQLGSTRTRADRDPREIDGLLINYHYLERNLGYTFKDRAYLLQALTHPSNPTNRLTGCYQELEFIGDAILDFLISAYIFERNIRMNPGQLTDLRSALVNNTTLACICVRHRFHLFILAENTLLTESINSFVDFQEKHMHRVTHHVRILLEENDIQCHNPLDEEDELEMADNLSTNDEPLAKGNLNMSENVDVPKALGDVVEALIAAVYLDCKDLQKTWEVVYRLFEPELIEFSRNVPINPLRQLNEHRLAKPIFSPAIMDEGKVMVSCLFTCNEKTIKVYGFGTNNSQAKLAAAKHALRKLAKSEA

Target ORF information:

RefSeq Version	XM_023175056.1
Organism	Drosophila willistoni
Definition	Drosophila willistoni endoribonuclease Dicer (LOC6637983), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023175056.1

ORF Insert Sequence:

ATGCCTGCTA ACGCGATGAC AACCCCACCC AAGGAAATGC AAGTACGTAG TTATCAATCG 
CGTCTGGTTG ACTATCTAAC TGAGCGCAAT GGGATAGTCT ATTTGCCCAC AGGAGCCGGC 
AAAACCTTTG TGGCCATCCT GACACTAAAA CGTTTCTCAT TGGACTTTGA TAAAACCATC 
GAGGATGGCG GAAAACGAGC TATATTCATG TGCAACACTG TCGAATTAGC CCGTCAACAG 
GCATTGGCTT GTAGATCACT TACAAATTTC AAAGTTGGAT TTTATGTAGG CGATCAAGGT 
GTCGATGAAT GGAGTAGATC CAAATGGGAT GTTGAAATAA GAGAAAAACA AGTTCTTGTT 
GGCACTGCCC AGGTCATGTT GGACCTGATG CTTCACGCCC ATCTGTCTCT GGGCTCCGTA 
AGCATTGTGA TTATAGATGA ATGTCATCAT GGCACCGGAC GTCATCCGTT TCGTGAATTT 
ATGCGTTTAT TTCTACACGT TCGGCCTAAT GTGCAACTGC CACGAGTTGT TGGGCTTACC 
GGAGTCTTGA TCAAGGGCAA TGAGATCAAC AATGTGAAAA AAATGTTGAA AAATCTGGAG 
ACAACATATC ATGGCAATAT TATCACCGTG TCCGATATGA AAGAAATGGA GAATGTCATG 
CTTTACTCGA CCAAGCCCAA GGAGTTTTTT TTGTTATATG GCTATGGAAC TCATAAATTT 
GAGCTCATAG ATACTATTAA ATATTACATT GAAGCGTTTC ACAAGAAGTT GGACAATTAC 
GATATTGGCA AACAACCAGT CCGTTTCTCC AATGGTCTGC AGCAGCAGCG GGAGCCAAAC 
AAGAAAAATT TTGCAAAAAG TCTACTTAAT GATTTTATTT ATCAACTGGA AGAGTATGGT 
ATCTACGTGG CGTCTATAGC AGTCGTTGCT TTACTAGTTG AGTTTGAAAT AAAGAAACGA 
CAGGCCGAGA CGCTGGTCTT AAGATCAATA TACCGAATGG CCATTAGTCT ATGTGATAAT 
ATTCGACATT TCCTTGTAAG CAAATTGAGG GAAGACATAG ATGATGATAT GTCTGATGAC 
AATGTGGACA CCGAAGAAAA CATTTACAAT TTTTCTACCC CTCGAGTGCA AAGATTTCTT 
CATTATTTAA AGCAAACCTT TAGCAACAAG AATCCAAAGG ATATATGCTG TCTAGTATTT 
GTAGAACGAA GGTACACATG TAAGAGCATC TACGGTATGC TTCTTAAGTA TATCGCCTCA 
GTGCCGGAGT TGAAGAATGT ACTGGTGCCG CAGTTTATGG TGGGACGGAA TGGAATCAAT 
CAAAATTTCG ATACCGTACT GGAACGAAAA TGGCAAAACT CAGCCATTCA ACAATTTCGA 
GATGGCGAGG CAAATCTAAT GATATGCTCA TCCGTATTAG AGGAGGGCAT CGATGTGCAG 
GCCTGTAATT ATGTTATTAT ACTGGATCCA TTGAAGACAT TTAACTCATA TGTGCAAACA 
AAGGGACGGG CTCGGTCTAA AGATGCTGTT TTTCTTATCC TAGCGTCGGA ATCTGAAAAG 
CCAAAGACTG CGAAGAGAAT TATGGACTAT CAGAATGCTC ATAGAGATAT AGCCAATTAT 
CTGCAGGATC GTGTCCTGGA GCGTGCCGAG CCAAAAATGG ATGAAATAAA CGAACATTTT 
AGAGATATAA TTCCGCCTTA TATCAATGAG ACAGGTTCTT TGCTTCTGGC CAGTAGTGCG 
CTAGCATTGC TACATCGTTA TTGTCAAACC TTACCATCCG ATGCATTTGG TTTCAATATG 
CCGTGGTACA AATTGCTTAA CCCAGACGAA CGTGTGCAAC ATTTTGGCAA AAGTGCTGAA 
ACTAAGCAAG TGGTTTCCAT TAATCTGCCA CTTAGCACCA AGCTAAGGAG TACCATATAT 
AGTGATCTTA TGAATTGCAA TAAATCAGCT AAAATTTCGG CTGCCTTCAA TGTGTGTAAG 
GAACTTTATA AACTTGGAGA GCTCAATCAA AAATTTCTGC CAACTACATT AACTGACCGC 
CTAAAAGATG TTTCTGATAT CCATTTTAAG CACTGGAAGA AATACGGCGA CGATGTAACC 
AAAACAAACC GTAAGGAAGA TAAATCAGGC AATACCAGCA AATATCCTGT CAACTGTCCC 
ACCCAATTGT ATGATACACG TCCTCGTGTG GGTGAGACCT GTTATGCTTA TGAGATATTA 
TTGAAGCCAC ATTTCGAGCG CAATGAATAC ACGGCCCATA TGCACGATGC CTTACAGACG 
GGGCGTGGGT ATGCCTTGCT CTTGCGTAAG CGACTACCAC CTCTGGCCGA AATGCCATTG 
TTCAGCAGTC ATGGAAAGAT TCATGTATCG GTCGCTGAGC AGCCGCATGA AGTGATTTTG 
AAAACTGAAG AGCAATTGGA TCATCTGCAT CAATTCCATA TTATGCTGTT TAGGGATATC 
TTGAAAATTT GGCAGTCCTA CTTTGTGCTT GATAAACGCA GCAAGGAGAA TTCCTATCTG 
GTGGTCCCCT TGGATATTAA ATCCAGCGGA AAAGGGGAGG GCAATATAAT AGATTGGAAT 
CTAGTGACGC AGTTCTATCG TGGTCTACCA CAATCAAGAA CTCTAAGCCC GGATCAGAGA 
CGCAGGCAAG AACCACCATG CCCGGATGAC TTTGAGGGTA AAATTGTGAC CCAATGGTAT 
GCAAGTTATC AAGATAAACG GATGTTGGTC ACCAAGGTGC ACAGGGATAA GACCCCATTT 
AGCATGATGG AAAGTAACCA AAGCGAAAAT AATTATTATG ACTACACCAT GTCCAAGTAT 
AAAAATGTTA TCGGAGATGT GGTCCATCAA AATCAGTGCC TTATCGAAGT TCGTGAGTTA 
ACACAGCAAT TAAATTTCTA TATAGCACAT CGAGTAAAAA CGTCGGGTCA GAGCAAAGCT 
CGTGCGCGAA TTATTTTAAT ACCCGAGCTA TGCTTCAATT TTCAATTTCC CGGCGATCTT 
TGGGCAAAAT TACTCTTTCT GCCAAGCATT CTGAATCGTT TGCATTACCT GCTTCATGCA 
GAGGCACTGC GTCAGCGTAT GAATACTTAC TTGGGACTAC ATGTGCTACC ACAAAATGGT 
CCAAATTATC GGCCCAAAGT GCTGGATGTC GATTTGTCGT TGCGCCGCAA TGTGGATTCT 
ACAGGAAATG CAGTTAGCAT GGAAGTTGAG GAACCACGCT CCATGTTGGA TCCATTGCCC 
ACCAAACAAT TAGAGGTATC AATGGATCGT CTTCAGATTA GTGATCTAGA AGCACCCTGG 
CAAAGTTATA TGGAACCAGT AGACTTGAGT CGCAACATGA TGTTGACTCA TCCAGTTGAA 
CTTGCTTATT ACTGTCAATT CACCAGCGGC CAGATTGTCA AATTTGACAA GATAGAGGAA 
GAGGACAAAG AATTCTGGAT GGAGACGCAA TTCAAAATGT CCAAGGGAAA TATTTACGAC 
TATAACACTA CGGCGAACGA ATCTCTACCA GCCCTGCTAC CCAACTCGAA TCCAATAACT 
CCAGTCGAGC AGCTATCGGT GTTGCAGAAA ACCATTTCCA ATGAACACAT CACTCCCGCC 
GAGCAGAGTG AATTTCTAAC AGCGATTACA AGTTCCGGAT GCTCGGATGT CTTTGATATG 
GAGCGTTTGG AATTGTTGGG TGATAGTTTT CTAAAGATGA GTGCAACATT ATATTTGTCA 
AGCATTCATA GCAACTGGAA CGAAGGCGTT CTCACCCAAA TAAAATCCAC ATTGGTGTCC 
AATCGCAATC TTTTATATTG CATGAACGAA ACCGATATTC CTTCGAGAAT CAATAGCACC 
TTGTTCACTC CCAAGTACAC CTGGAAACCG CCTGGCCTTA GTTTGCCACA TAATATATTA 
AATCTATGGC AGGAACAGCC AGAAGTCGCC AAATTTGTAT TACCAAGAAA TCTGTGTGCC 
CTACAACTTA CTGAGGAAGA GATTTTCTCA TTGGGAAAAT TAACCCCGGC AAACTTTGAA 
AAGTTTTTGG AAAGCTGTCA GGCCAACACG AATTACACCA GTACGGATTA TGAATCTGAA 
ATGAATTTCT GTATCGGTAA ATCAAAACTG TCAAACAAAG TGGTCGCCGA CACGTTGGAA 
GCTCTCCTTG GCGTTATTGT TAAGAACTAT GGTGTTCAGC ATGGTTTCCG CATTTTGGAA 
TATTTTGGCA TCTGCAAGCA GGTAGACATT GGCAAGCCAT TGTCCCAACT TCTTGACCTT 
CAATTGGGTA GCACTCGTAC ACGAGCGGAT CGTGATCCTC GTGAGATTGA TGGACTACTC 
ATCAATTATC ATTATTTGGA GCGAAATCTT GGATACACCT TCAAAGATCG CGCCTATTTA 
CTACAGGCTC TCACACATCC ATCGAATCCC ACTAACCGTT TGACTGGCTG CTATCAAGAG 
TTGGAGTTTA TTGGTGATGC CATTTTGGAT TTCCTAATCT CAGCATATAT TTTTGAGCGA 
AATATCCGCA TGAATCCTGG CCAACTGACA GATTTGCGAT CCGCTTTGGT CAATAACACA 
ACATTGGCCT GCATTTGTGT TAGGCATCGG TTCCATTTAT TCATTTTGGC TGAAAATACA 
CTACTTACCG AATCCATCAA TAGTTTTGTG GATTTCCAGG AAAAACATAT GCACCGTGTA 
ACCCATCATG TGCGAATACT TCTTGAAGAA AACGATATAC AATGTCACAA TCCCTTGGAC 
GAGGAAGATG AATTGGAAAT GGCCGACAAT TTGTCTACCA ACGATGAACC GCTAGCCAAG 
GGTAACTTGA ATATGTCGGA AAATGTAGAT GTGCCCAAGG CCTTGGGTGA TGTTGTAGAA 
GCCTTAATTG CAGCCGTGTA TCTTGATTGC AAGGATTTAC AAAAAACCTG GGAAGTGGTA 
TACAGACTCT TTGAGCCTGA ACTTATTGAA TTCTCACGCA ATGTGCCTAT AAATCCTCTT 
CGTCAACTCA ATGAGCATAG GCTAGCTAAA CCGATATTCA GTCCTGCCAT TATGGATGAG 
GGTAAGGTGA TGGTTAGTTG CTTATTCACC TGCAATGAGA AAACCATTAA GGTCTATGGA 
TTTGGTACCA ACAACAGCCA AGCTAAGCTG GCGGCAGCAA AACACGCTCT ACGCAAACTT 
GCCAAGAGCG AAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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