Dpse\GA30272 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA30272
Entrez Gene ID	6899052
Full Name	GA30272 gene product from transcript GA30272-RB
Synonyms	Dpse\GA21759,Dpse\GA25110,Dpse_GA21759,Dpse_GA25110,GA21759,GA25110,GA30272,dpse_GLEANR_16371,dpse_GLEANR_16372
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_002138941.3	XP_002138977.3	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA30272 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA30272 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe22179	XM_002138941.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA30272), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA30272 ( XM_002138941.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002138977.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA30272), partial mRNA.

Price & Availability↑

CloneID	ODe22179
Clone ID Related Accession (Same CDS sequence)	XM_002138941.3
Accession Version	XM_002138941.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_002138941.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGCGCGCCA AAACGGCTCA CAGCAGTCAC TATCGATATT TATATCGATA TTTTGCCCCT 
GCCCAGCACT GGCGGGCAAA TAAACAACCG TGGAAAAAAG AGGGGCAAAA AAACAACAGC 
AAAGCGTGTA AAAAAAAGAA AACAAACACA CAAACACGAA TTCAAGGCGG AAACGCAATC 
GGCGTTGCCA CTTCGCTGAG CTGCGCGGAC TCCAGCACGG GCTCCAGTGA GGATGCGTCC 
GAGCCCGGCT CCCCGTACAG TGCGCACTCG CAACTGAGCG ACGAACAGCT GACCAAGATG 
CCGCCCCAGA TACTGAGCGG GAGCAGTAGC ACTCCCAGCG GCCCGAACAG CTCCTCCACA 
GCGTCCTCCA CCTCCACATC CCACAGCAGC CCGACAGCAG CGCCGGCGAC GCCTCCAGCT 
TCGGCCATTT CCACCCACGG CCACGCCCAC ATCCACAGCC TCAGCATCAG CACTGCCAAA 
CCCACAACGA TGCCGATGGC CATGGCTAAC TCCCAGGGGC GACAGCCCAG CCTGCTGCCC 
ACCCTCCAGT GGCCGTGGAA CACGAATCTG GGCAGCACCT CCACCGCCGT GCCCGCCGTA 
GCGGCCAACA AGAAGCGTCC AGCTGGAGGA TCGGGACCGG GCGCCGGCGT CGCAGAGGGG 
AGCAGTCCGG CCTCGGCGGC GGCCAAGAAA AGTCGCTGCG AGCCCCAGGC AGGTAGCGAC 
GCGAGCAAAA GACTGAAGGC GGCGGCCCTT GAGGAGTCGC AGACGAAGAT CACCGGTTTC 
TTCAAGTCCC AGATGAAGCC CTCACCGGGG GGCAAGCTGT CGCCGCCACC CAACGCCATC 
GCCAGCACAG CCCCTGCCCT GCACCCGGCC AACACGCTGA CTATGAGCAC GCCCGCCACG 
ACCGCCTCCT TGAACAAGTA CTTTAACATT TTGTCGCAGC TCAAGGAGCA GAAACAGCAG 
CAACAGCAGC AGCTGCAGAT GCAACAGCAG GCGACAAAAC AGGCAACGCC CAGCCCCGCT 
GTCCTGGCGC CGCCACAGCC GATTGTGAGT GCAACGACAC AGGCGCCACC GCCAGCGTCA 
CTGCCACTGC CACTGCCAGC CAGCCAGACG ACTAGTCTGC CCGTTCCGGC TCTCAAGAAG 
ATTGAGCGGA GCACCAAGCA GCCGGCGAAG ATCGCCCAGG TGGCGCCCAA CCTACGCAAG 
ACGCCCAGCA ACGGCAGCAA CCACAGCAAC TCCTCCTCCA GCTCCTCGTC CTCCACCTCC 
TCAAACGGCT CCAACAGCTC CCACACGGGG GTGGGCAAGT CGCCGGCCAA GAAGCACGTG 
GCCATCGCAC CGCGAACGCC CGAGATGAAG CAGCAGCAGC AGCAACAACA GCAGCTCCAG 
CTGCAGGCGC AGGCCGGAAA GGCGGCCATG TCCTACCGTC CGCCGGGCAC GAGTCCGGCG 
CTGAAGCAGA AGATCTCTCC GGCCCCAGGG CCAGCCACCA ACACTGCCGC GTGCACCGCC 
ATCACGCATC CGACGACGCC GGCCGCCCCG AGCCCCAACG CCAATCCCAA CCCCAATGCG 
ACGCTCTACC AGCTGCCCGT GCAGCTGCCG AATCTAGTCC AACTGCCGCC CCAACTGGCG 
GCCGCCGCCA ACATAATGCA GCTGAACAAC GTGGCCAAGG CGGCGATGGC CACGGCAGCG 
AACAACAATG CGGCCGCCGC ACAGGCGGCC CAAGCGGCCC AGGCAGCCGC CGCCCAGTAC 
TTCCTCAACG GAACGGTGTT CAAGCTGCAG CAGGTGACAA CGGCGACGAC TACGACGGCC 
ACCACGGCCA CGGCAGCGGC CTCCACGGGC AATCCCTTCG GTCTGCTCAG CGCCAACGAG 
CTGCAGGACC TGCTGATGAA GCAGCAACAG CAGCAACAGC AACAGCTCCA GCAGCAGCAG 
CAGCAGCTGC AACTGCAGCA GGCGGCTGCG GCCAAGGCAG CGGCGACCAC GCCCTCGCCC 
AACAGTGCGC CCAACTTCCA GGAGCTGTTC CAACAGCAGA TTGCTGCCGC CATCCAGCAG 
CAGCAGCTGC AGCGACTGGT GCAGGGAGGA GCCGCCGCCG CTGCGGCCCA GCCAGTGTTC 
ATGGCCACGC CGGCGGGTCT CCTGCTCAAC GCTGCCACGC TGCCTGCCAT GCTGGCCCAG 
GCGGCGGCAG CCATTGCGGC CAACAATCAG CAGCAGCAGC AACAGCAACA ACAACAGCAG 
CAGCAGCAGC AGCAAAAGGT GGCCGCCACC CTGGCCCTAC AGCATCATCA ACATCATCAG 
CAACAGCAAC ACCAGAGCCT GCCCGCCCTG CAGCCCATGC CCGCCCTGAG CTCCATCTTC 
GAGCCTTCGA CGGAGCAGCA GCAGCAGCAG CAACAGCAAC AGCTGCAGAT GCAACAACTG 
ATCACTGCCA GCAACAACCA TAATGCCTCG AATAATCTAA GTAGCATCAA CAGCACTGCC 
ATGCAACTGC ACCAGCAGCA GCAGCAGCAG CTGCAACACC AACACCAACA CCGGGCGATT 
GTGAAGCCTC CGAGGCTCAG CTCCAGCCAG CCCCCGCCTC TGGTGACCAT CTCGAACTCA 
TCCATGGCCA CCAAGCCGCC GATCCTGCCG CGTCGCAAAG TGACGAGCAC GTCTTCCACC 
ACGTCGCAGA TCTTCGGACT GCGGCCGCCC ACCGATGATG TTGACCTCAA GAGCAGGACA 
CGCTCCCTGC CACCCAAGAC GGCCCCTGCC ATTGCCACGC CCACGACACC ACCGCCACTA 
GTGCCCACAT CGGCCACCAA AGAACTGTGT CAGCTGCGGA AGAGCACGAC CCCAGTGGCA 
TCCGTAGCCA CGGTCGCGCT CGCGCTGGCG CCCACTCCCA CGACATGTAA TCCCACGCCG 
CCGCTGGTGA GCATTGCGCC CTCGAAGCTG ACGCCCACGT TGAGCATGGC GAAGGTCCAG 
GTGCCGGCCC TAAAGTTGGG CACCAGCACC AGCAGCAGCA GCAGCAGTAG CAGTAGCATC 
AGTGGCGGTG GCGGTGGCGG TGGCGTCACA CCCGATCTCT TTGACCTGGT CAAGAACTCG 
CACGGAGCCA TCAGCATCGT GGCCAAGGCG AGCAGCCATC CGCTAACGCC GCTGCCCTTC 
AGTTCGCCGA GCAGCAACAG CAGCAGCAGC CTCCTGCGAT CCTCGCCGGC GCTGTCCAGC 
TCCAACTCGT GCACGCTGTC GGCCTTCAGC AAGATCAAGG CGGAGCCCTC GGAGGATTCG 
GCCTCTGCCC TCGTGGCGGC AGTCTCGCTA TGCTCCTCGG TCTCCTTCCT GGCCTCGCCC 
AAGCCGCAGA GCTTTGCCGG CCAGCTGCCT AGCAGCAGGG GCACGGCGGA CGTGGTGAAG 
CACGACCTGC TGCCGGAGTG CAGCAACAGC AACTCGAACT CCAACTCCTG CAGCAGCTAC 
TCCAACAGCG TCAGCTCCGC GGCGGATCTC TCGCTGGAGC CCTCGACGCC CGCCTCCCTC 
AGCTCGCCCT CGCCGGCGCC GTCGCCCTCG GCTGGCAGCA GCAGCAGCCG GAGGGCTGCC 
AGTCAGACGG ACATGCTCAG CGAAATGGTC ACCTCGTCGT GCATCTCCAG CGGGGGCGAG 
GACTGCTCCC AGACGACCAC AGACATGACA CAGCCGCCGC CGGAGAAGAG CGAGCCGACG 
GCCACGCCGC CTCTGATCGC CAGCAGCAAC AGCGGAGGAA GCAGCAGCAG CGGGAGCAGC 
AACTACGACG AGGAGGACGA CAAGTCGGTG GGCTCCCTGG AGACGCATCC GACGCACAAG 
CGGCTGCGGG ATCTGCCCAC GCCCGAGTCC GGGATCGGGG GCAGCCTGAG CAACAGCGAG 
AGCAGCAACT CCATCGCGGA TGGCATGTCT ACCAAATCCG TCTCGGCCTG CCCGCCCACC 
ACTGCCTCGT CCGCGTCCGC GTCTGCGTCC GCTTCCGGCT CGGACAGCAA CTCCCTGGCC 
AGCAACGCAC CATCGCCCGA AGCTCCAGAC TCCCTAGACT CGAGCTCCCC GTGCCCCACG 
CCAGCCAGCA GCACGGAGTC CACGATGGTG GACACGGCCC TGGCCGAATT CGCCAGCAAG 
AGCCTGCCCA AGTGCGCCAT TTCGCCGATC CTCTCGCAGC CAAAGACCAT CCGGTTCCCG 
GCTGGAGCCG GCGCCGGCGG CAAGGGCGGA AAGCGGCACG ACGGCGTCTG CTACTGGGAC 
AAGTGCAACA AGAAGCACGA GAGCAACTCG AAGCTGCTGG ACCACATGCA GACGCACCAC 
GTGAACACCC AGACGGGGCC CTTCGCCTGC CTCTGGGTGG GCTGCAAGGT GTACAACAAG 
GAGTCCTGCT CGCGGCGCTG GCTGGAGCGC CACGTCCTCT CCCACGGCGG CTCCAAGCAG 
TTCAAATGCA TTGTCGAGGG CTGCGGAATA AGGTTCGGTT CACAGCTGGC TCTGCAGAAG 
CATGTGAACA ACCACTTCAA TGCCACTGAC AACGTCAAGG AGAGCGCCAG CAAGCGCACC 
TCCGATCCCC CCGTGCCCAA GCAGCTGCGG AAGAACGGCA AGAAGCTGCG CTACCGCCGA 
CAGCCCTTCT CAGCTCGCAT GTTCGACTTC TTCGACACGG GCATCATGGA GGGGCTGCAG 
CATCGCCTGC GCCAGATCAG CACGCTCACC AACGGCTCCC AGGCCATCAC CTTCCAGGGC 
CAGTGCCTGA TGCGGAGGCG CAACAGCCAG GGTGGCCACG ATTGCTTCGT GCGTTGGTCC 
CCGCGCGAGA TAATATCTGA CGAGTGGTTG CCCGAGTGTC CGAATCGGAC GCGCTGCCAC 
ACGAAGGTGG TGCAGATCAA GCACATGCGT CCAGCGGAGA AGACCCGCGT GGACAGCCTT 
CTGAGTACGG CCTTTAGGCT GCGCTACGAC GCCCAGCTCT TTGCCGACGA CTACAATGTG 
AACGAGCTGC AGGAACTGGG CGCGGTGACT AGTGACTGCG AGGATGACGA TGAGGAGGAC 
GAGAATGATG AAGAGGACGA TGAGGAGGAG GAGGACGATG GCGTCTCTGC GACGGTCAGC 
TCGAGGACTG CCAGCAGTGG GACCGTGTCC AGCTACCAGC AGGTGCTCAG CATTGCCAAA 
CTGCAGATGC AGCAGCGTCG CAAGCACCCC CGCAAACCGC CCAAGATGGT GGCCACGGAT 
GCTCTGGTGC CCATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002138977.3
CDS	1..5178
Translation	MRAKTAHSSHYRYLYRYFAPAQHWRANKQPWKKEGQKNNSKACKKKKTNTQTRIQGGNAIGVATSLSCADSSTGSSEDASEPGSPYSAHSQLSDEQLTKMPPQILSGSSSTPSGPNSSSTASSTSTSHSSPTAAPATPPASAISTHGHAHIHSLSISTAKPTTMPMAMANSQGRQPSLLPTLQWPWNTNLGSTSTAVPAVAANKKRPAGGSGPGAGVAEGSSPASAAAKKSRCEPQAGSDASKRLKAAALEESQTKITGFFKSQMKPSPGGKLSPPPNAIASTAPALHPANTLTMSTPATTASLNKYFNILSQLKEQKQQQQQQLQMQQQATKQATPSPAVLAPPQPIVSATTQAPPPASLPLPLPASQTTSLPVPALKKIERSTKQPAKIAQVAPNLRKTPSNGSNHSNSSSSSSSSTSSNGSNSSHTGVGKSPAKKHVAIAPRTPEMKQQQQQQQQLQLQAQAGKAAMSYRPPGTSPALKQKISPAPGPATNTAACTAITHPTTPAAPSPNANPNPNATLYQLPVQLPNLVQLPPQLAAAANIMQLNNVAKAAMATAANNNAAAAQAAQAAQAAAAQYFLNGTVFKLQQVTTATTTTATTATAAASTGNPFGLLSANELQDLLMKQQQQQQQQLQQQQQQLQLQQAAAAKAAATTPSPNSAPNFQELFQQQIAAAIQQQQLQRLVQGGAAAAAAQPVFMATPAGLLLNAATLPAMLAQAAAAIAANNQQQQQQQQQQQQQQQQKVAATLALQHHQHHQQQQHQSLPALQPMPALSSIFEPSTEQQQQQQQQQLQMQQLITASNNHNASNNLSSINSTAMQLHQQQQQQLQHQHQHRAIVKPPRLSSSQPPPLVTISNSSMATKPPILPRRKVTSTSSTTSQIFGLRPPTDDVDLKSRTRSLPPKTAPAIATPTTPPPLVPTSATKELCQLRKSTTPVASVATVALALAPTPTTCNPTPPLVSIAPSKLTPTLSMAKVQVPALKLGTSTSSSSSSSSSISGGGGGGGVTPDLFDLVKNSHGAISIVAKASSHPLTPLPFSSPSSNSSSSLLRSSPALSSSNSCTLSAFSKIKAEPSEDSASALVAAVSLCSSVSFLASPKPQSFAGQLPSSRGTADVVKHDLLPECSNSNSNSNSCSSYSNSVSSAADLSLEPSTPASLSSPSPAPSPSAGSSSSRRAASQTDMLSEMVTSSCISSGGEDCSQTTTDMTQPPPEKSEPTATPPLIASSNSGGSSSSGSSNYDEEDDKSVGSLETHPTHKRLRDLPTPESGIGGSLSNSESSNSIADGMSTKSVSACPPTTASSASASASASGSDSNSLASNAPSPEAPDSLDSSSPCPTPASSTESTMVDTALAEFASKSLPKCAISPILSQPKTIRFPAGAGAGGKGGKRHDGVCYWDKCNKKHESNSKLLDHMQTHHVNTQTGPFACLWVGCKVYNKESCSRRWLERHVLSHGGSKQFKCIVEGCGIRFGSQLALQKHVNNHFNATDNVKESASKRTSDPPVPKQLRKNGKKLRYRRQPFSARMFDFFDTGIMEGLQHRLRQISTLTNGSQAITFQGQCLMRRRNSQGGHDCFVRWSPREIISDEWLPECPNRTRCHTKVVQIKHMRPAEKTRVDSLLSTAFRLRYDAQLFADDYNVNELQELGAVTSDCEDDDEEDENDEEDDEEEEDDGVSATVSSRTASSGTVSSYQQVLSIAKLQMQQRRKHPRKPPKMVATDALVPI

Target ORF information:

RefSeq Version	XM_002138941.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA30272), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002138941.3

ORF Insert Sequence:

ATGCGCGCCA AAACGGCTCA CAGCAGTCAC TATCGATATT TATATCGATA TTTTGCCCCT 
GCCCAGCACT GGCGGGCAAA TAAACAACCG TGGAAAAAAG AGGGGCAAAA AAACAACAGC 
AAAGCGTGTA AAAAAAAGAA AACAAACACA CAAACACGAA TTCAAGGCGG AAACGCAATC 
GGCGTTGCCA CTTCGCTGAG CTGCGCGGAC TCCAGCACGG GCTCCAGTGA GGATGCGTCC 
GAGCCCGGCT CCCCGTACAG TGCGCACTCG CAACTGAGCG ACGAACAGCT GACCAAGATG 
CCGCCCCAGA TACTGAGCGG GAGCAGTAGC ACTCCCAGCG GCCCGAACAG CTCCTCCACA 
GCGTCCTCCA CCTCCACATC CCACAGCAGC CCGACAGCAG CGCCGGCGAC GCCTCCAGCT 
TCGGCCATTT CCACCCACGG CCACGCCCAC ATCCACAGCC TCAGCATCAG CACTGCCAAA 
CCCACAACGA TGCCGATGGC CATGGCTAAC TCCCAGGGGC GACAGCCCAG CCTGCTGCCC 
ACCCTCCAGT GGCCGTGGAA CACGAATCTG GGCAGCACCT CCACCGCCGT GCCCGCCGTA 
GCGGCCAACA AGAAGCGTCC AGCTGGAGGA TCGGGACCGG GCGCCGGCGT CGCAGAGGGG 
AGCAGTCCGG CCTCGGCGGC GGCCAAGAAA AGTCGCTGCG AGCCCCAGGC AGGTAGCGAC 
GCGAGCAAAA GACTGAAGGC GGCGGCCCTT GAGGAGTCGC AGACGAAGAT CACCGGTTTC 
TTCAAGTCCC AGATGAAGCC CTCACCGGGG GGCAAGCTGT CGCCGCCACC CAACGCCATC 
GCCAGCACAG CCCCTGCCCT GCACCCGGCC AACACGCTGA CTATGAGCAC GCCCGCCACG 
ACCGCCTCCT TGAACAAGTA CTTTAACATT TTGTCGCAGC TCAAGGAGCA GAAACAGCAG 
CAACAGCAGC AGCTGCAGAT GCAACAGCAG GCGACAAAAC AGGCAACGCC CAGCCCCGCT 
GTCCTGGCGC CGCCACAGCC GATTGTGAGT GCAACGACAC AGGCGCCACC GCCAGCGTCA 
CTGCCACTGC CACTGCCAGC CAGCCAGACG ACTAGTCTGC CCGTTCCGGC TCTCAAGAAG 
ATTGAGCGGA GCACCAAGCA GCCGGCGAAG ATCGCCCAGG TGGCGCCCAA CCTACGCAAG 
ACGCCCAGCA ACGGCAGCAA CCACAGCAAC TCCTCCTCCA GCTCCTCGTC CTCCACCTCC 
TCAAACGGCT CCAACAGCTC CCACACGGGG GTGGGCAAGT CGCCGGCCAA GAAGCACGTG 
GCCATCGCAC CGCGAACGCC CGAGATGAAG CAGCAGCAGC AGCAACAACA GCAGCTCCAG 
CTGCAGGCGC AGGCCGGAAA GGCGGCCATG TCCTACCGTC CGCCGGGCAC GAGTCCGGCG 
CTGAAGCAGA AGATCTCTCC GGCCCCAGGG CCAGCCACCA ACACTGCCGC GTGCACCGCC 
ATCACGCATC CGACGACGCC GGCCGCCCCG AGCCCCAACG CCAATCCCAA CCCCAATGCG 
ACGCTCTACC AGCTGCCCGT GCAGCTGCCG AATCTAGTCC AACTGCCGCC CCAACTGGCG 
GCCGCCGCCA ACATAATGCA GCTGAACAAC GTGGCCAAGG CGGCGATGGC CACGGCAGCG 
AACAACAATG CGGCCGCCGC ACAGGCGGCC CAAGCGGCCC AGGCAGCCGC CGCCCAGTAC 
TTCCTCAACG GAACGGTGTT CAAGCTGCAG CAGGTGACAA CGGCGACGAC TACGACGGCC 
ACCACGGCCA CGGCAGCGGC CTCCACGGGC AATCCCTTCG GTCTGCTCAG CGCCAACGAG 
CTGCAGGACC TGCTGATGAA GCAGCAACAG CAGCAACAGC AACAGCTCCA GCAGCAGCAG 
CAGCAGCTGC AACTGCAGCA GGCGGCTGCG GCCAAGGCAG CGGCGACCAC GCCCTCGCCC 
AACAGTGCGC CCAACTTCCA GGAGCTGTTC CAACAGCAGA TTGCTGCCGC CATCCAGCAG 
CAGCAGCTGC AGCGACTGGT GCAGGGAGGA GCCGCCGCCG CTGCGGCCCA GCCAGTGTTC 
ATGGCCACGC CGGCGGGTCT CCTGCTCAAC GCTGCCACGC TGCCTGCCAT GCTGGCCCAG 
GCGGCGGCAG CCATTGCGGC CAACAATCAG CAGCAGCAGC AACAGCAACA ACAACAGCAG 
CAGCAGCAGC AGCAAAAGGT GGCCGCCACC CTGGCCCTAC AGCATCATCA ACATCATCAG 
CAACAGCAAC ACCAGAGCCT GCCCGCCCTG CAGCCCATGC CCGCCCTGAG CTCCATCTTC 
GAGCCTTCGA CGGAGCAGCA GCAGCAGCAG CAACAGCAAC AGCTGCAGAT GCAACAACTG 
ATCACTGCCA GCAACAACCA TAATGCCTCG AATAATCTAA GTAGCATCAA CAGCACTGCC 
ATGCAACTGC ACCAGCAGCA GCAGCAGCAG CTGCAACACC AACACCAACA CCGGGCGATT 
GTGAAGCCTC CGAGGCTCAG CTCCAGCCAG CCCCCGCCTC TGGTGACCAT CTCGAACTCA 
TCCATGGCCA CCAAGCCGCC GATCCTGCCG CGTCGCAAAG TGACGAGCAC GTCTTCCACC 
ACGTCGCAGA TCTTCGGACT GCGGCCGCCC ACCGATGATG TTGACCTCAA GAGCAGGACA 
CGCTCCCTGC CACCCAAGAC GGCCCCTGCC ATTGCCACGC CCACGACACC ACCGCCACTA 
GTGCCCACAT CGGCCACCAA AGAACTGTGT CAGCTGCGGA AGAGCACGAC CCCAGTGGCA 
TCCGTAGCCA CGGTCGCGCT CGCGCTGGCG CCCACTCCCA CGACATGTAA TCCCACGCCG 
CCGCTGGTGA GCATTGCGCC CTCGAAGCTG ACGCCCACGT TGAGCATGGC GAAGGTCCAG 
GTGCCGGCCC TAAAGTTGGG CACCAGCACC AGCAGCAGCA GCAGCAGTAG CAGTAGCATC 
AGTGGCGGTG GCGGTGGCGG TGGCGTCACA CCCGATCTCT TTGACCTGGT CAAGAACTCG 
CACGGAGCCA TCAGCATCGT GGCCAAGGCG AGCAGCCATC CGCTAACGCC GCTGCCCTTC 
AGTTCGCCGA GCAGCAACAG CAGCAGCAGC CTCCTGCGAT CCTCGCCGGC GCTGTCCAGC 
TCCAACTCGT GCACGCTGTC GGCCTTCAGC AAGATCAAGG CGGAGCCCTC GGAGGATTCG 
GCCTCTGCCC TCGTGGCGGC AGTCTCGCTA TGCTCCTCGG TCTCCTTCCT GGCCTCGCCC 
AAGCCGCAGA GCTTTGCCGG CCAGCTGCCT AGCAGCAGGG GCACGGCGGA CGTGGTGAAG 
CACGACCTGC TGCCGGAGTG CAGCAACAGC AACTCGAACT CCAACTCCTG CAGCAGCTAC 
TCCAACAGCG TCAGCTCCGC GGCGGATCTC TCGCTGGAGC CCTCGACGCC CGCCTCCCTC 
AGCTCGCCCT CGCCGGCGCC GTCGCCCTCG GCTGGCAGCA GCAGCAGCCG GAGGGCTGCC 
AGTCAGACGG ACATGCTCAG CGAAATGGTC ACCTCGTCGT GCATCTCCAG CGGGGGCGAG 
GACTGCTCCC AGACGACCAC AGACATGACA CAGCCGCCGC CGGAGAAGAG CGAGCCGACG 
GCCACGCCGC CTCTGATCGC CAGCAGCAAC AGCGGAGGAA GCAGCAGCAG CGGGAGCAGC 
AACTACGACG AGGAGGACGA CAAGTCGGTG GGCTCCCTGG AGACGCATCC GACGCACAAG 
CGGCTGCGGG ATCTGCCCAC GCCCGAGTCC GGGATCGGGG GCAGCCTGAG CAACAGCGAG 
AGCAGCAACT CCATCGCGGA TGGCATGTCT ACCAAATCCG TCTCGGCCTG CCCGCCCACC 
ACTGCCTCGT CCGCGTCCGC GTCTGCGTCC GCTTCCGGCT CGGACAGCAA CTCCCTGGCC 
AGCAACGCAC CATCGCCCGA AGCTCCAGAC TCCCTAGACT CGAGCTCCCC GTGCCCCACG 
CCAGCCAGCA GCACGGAGTC CACGATGGTG GACACGGCCC TGGCCGAATT CGCCAGCAAG 
AGCCTGCCCA AGTGCGCCAT TTCGCCGATC CTCTCGCAGC CAAAGACCAT CCGGTTCCCG 
GCTGGAGCCG GCGCCGGCGG CAAGGGCGGA AAGCGGCACG ACGGCGTCTG CTACTGGGAC 
AAGTGCAACA AGAAGCACGA GAGCAACTCG AAGCTGCTGG ACCACATGCA GACGCACCAC 
GTGAACACCC AGACGGGGCC CTTCGCCTGC CTCTGGGTGG GCTGCAAGGT GTACAACAAG 
GAGTCCTGCT CGCGGCGCTG GCTGGAGCGC CACGTCCTCT CCCACGGCGG CTCCAAGCAG 
TTCAAATGCA TTGTCGAGGG CTGCGGAATA AGGTTCGGTT CACAGCTGGC TCTGCAGAAG 
CATGTGAACA ACCACTTCAA TGCCACTGAC AACGTCAAGG AGAGCGCCAG CAAGCGCACC 
TCCGATCCCC CCGTGCCCAA GCAGCTGCGG AAGAACGGCA AGAAGCTGCG CTACCGCCGA 
CAGCCCTTCT CAGCTCGCAT GTTCGACTTC TTCGACACGG GCATCATGGA GGGGCTGCAG 
CATCGCCTGC GCCAGATCAG CACGCTCACC AACGGCTCCC AGGCCATCAC CTTCCAGGGC 
CAGTGCCTGA TGCGGAGGCG CAACAGCCAG GGTGGCCACG ATTGCTTCGT GCGTTGGTCC 
CCGCGCGAGA TAATATCTGA CGAGTGGTTG CCCGAGTGTC CGAATCGGAC GCGCTGCCAC 
ACGAAGGTGG TGCAGATCAA GCACATGCGT CCAGCGGAGA AGACCCGCGT GGACAGCCTT 
CTGAGTACGG CCTTTAGGCT GCGCTACGAC GCCCAGCTCT TTGCCGACGA CTACAATGTG 
AACGAGCTGC AGGAACTGGG CGCGGTGACT AGTGACTGCG AGGATGACGA TGAGGAGGAC 
GAGAATGATG AAGAGGACGA TGAGGAGGAG GAGGACGATG GCGTCTCTGC GACGGTCAGC 
TCGAGGACTG CCAGCAGTGG GACCGTGTCC AGCTACCAGC AGGTGCTCAG CATTGCCAAA 
CTGCAGATGC AGCAGCGTCG CAAGCACCCC CGCAAACCGC CCAAGATGGT GGCCACGGAT 
GCTCTGGTGC CCATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA30272 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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