Dpse\GA30445 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA30445
Entrez Gene ID	6898867
Full Name	GA30445 gene product from transcript GA30445-RC
Synonyms	Dpse\GA15059,Dpse\GA24173,Dpse_GA15059,Dpse_GA24173,GA15059,GA24173,GA30445,dpse_GLEANR_13894,dpse_GLEANR_13895
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_004444388.2	XP_004444445.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA30445 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA30445 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe22072	XM_004444388.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA30445), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA30445 ( XM_004444388.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_004444445.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA30445), mRNA.

Price & Availability↑

CloneID	ODe22072
Clone ID Related Accession (Same CDS sequence)	XM_004444388.2
Accession Version	XM_004444388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_004444388.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGTAG AGAACTTGAA AACACCGCCA ACGCAGCTTC GTAATTTGGT CAAACACGTG 
AACAAGCGAA ATTTTAATGA AACCAGCGAG CAAATAAACC AGTTCATCAA AGACCACGGC 
CTGGAAGCTG ATCGTAGCAG TCTGCGCTAT CTGTTCACTG CGATCAACTT CAGTGACCCA 
TCGCCGACCG TCACCGTCCA GTTGCAAGCC AAATTACTCG GTGGCCAGTT GCAGCGACAG 
TTGCAAAGCT CCTCGTTTGT GTCCAACATC TGCTTCGCGT TTGACCAGCA CTTTGCCAAC 
AGCCAAAAGT CTCTCAAGCC GTCTGAGACA GCAGAGCTCA TTGGCCAGGT GTGCAGACTG 
ACCGGCATAA ACAAGCTCTG CGAGTGCGTA TTTGCCTTGG CGGTGACCCA CTCATCGCAT 
CCAGAGCTTC GAGAATCGGC CAGAAACTGC TTGCGAGGCT CCATAACCGA GCTGCTCGAT 
TCCTATTTGG GGAACAACAA CAGCTCCTCA GCTACCAGTG GGCTGCAAGA TATATCGATC 
GATCTTCTGC AGTATTTGCT GTGCTGCCTC AAAATTTACG TGACGCCGAA GCTGCACGAG 
CAATTTTTGA TCAAACTACG TGAGGAGTTT CCGCGGCAGG CGGTGCCTTT GATTTTGGCG 
CCATTCCTTT ACGGCTCAAC GACGGCGGCG ACCTCGAATC CAGTTGGGGC CAACGCCAGC 
GAAACTGATG CAGAGGCTGA GGCAACGACA ACGACAGAGA CAGAGACGGC CAGCAGCAGC 
ATCAGCCCCA GCACCAGCAG CAACAGTTGC GGCACGGATG CCTTGAATGA GGTTGGAATC 
GAGGATATCT ATGACCATTT AAGCGAAATA ATATTCACAA ACCAGGGTAA AAATAATATT 
ATGGACACAT CTTGGATTAA TCTAATCCTT GAAATCGGTT ACGAATTTAC ATCGAGCGTC 
GAAGAGTGTA AAAATCACCT GTGCTCTCGC GAACGAGAGC GCACCGAACT CCAGCCAAAA 
GATGTTGCCA AAATCGTTGG TCTCATGTGC CGGCGGCACT CGTCTTTGCT CGATTGTAAT 
GTTAATCTAC CGACGCCAGC TAATTTCTGG CCTGGACAAG GTCAAGGCGG TGGCAGCAAC 
AGTAGCGGGG GCTCGTCACA AGCACAGGCC ACCCCAGCGC AGCAGGGACA GAACTCAAGC 
AGCAACAGTA ACAACAACGA TGGAGCCAGC GACGGTGGAG GCAGCTCGTC CAGTGATAAG 
AAGGACAAGA AGGAGACGAC AGATGCCACA CAGACATGGA AGCCGGATGT CTTTGTGCAG 
GCACTCAAGG AACTGGTGCC ACAGCTCAAC TGGAAGGACG TTTGCATTGA GCTCGATCAT 
CCCGAGTTTG TGCTGAAGGA TCGCATCGGC TTGGACCTGC TGTTGACAAT TCTGCGACTG 
GCCACACAGT CGAACATCTT TCCACATCCA GAATGCATTT ACCGCCACTG GGCCAACACG 
GAGGGTCAGC TGTCGCTGAT CTCTACGCTG CTAAAGAATC CGGATCTGTT CTCCTTTGCC 
GACTTTGTGT TCAGTCAGCC AACGCTGGAT GTCCTGAAGA CGGCACCGGA TGCGGACAAT 
AAGGAGATTT CGGCATGGAA GTCCCTCCAC CTTGTCGAGG TCCTGCTCTC TATTGCGGAC 
AAGGGCTACT ACACGCAGGT CCATGAGCTA TTCAAGTTCC CTGCACAGAA CTGCCCGGAC 
GTCCTGTTTT TGGCACTGCT GCACATAAAT CCACCACTGA CACCGCTACG GCAGGACTTG 
TTCAATTTGC TGATACCGAC GTTCCTCAAC AATCATCCCA ATTCGAATGT GATCCTGGCC 
AGCGCCTGGA GCTCGACCAA CTTCTCGCTG CGGCCGAACA TTATGAACGC CATGTCCGAG 
TGGTATCTGC GCAGCAGCGA CTTTGATCAA GTAAAGCTGT CGCGCATTCT CGATCTCGCG 
CAGGACCTGA AGGCCCTCTC GTCGCTCCTG AATGCACGCT CCTTTCTGTT CATCATCGAT 
CTGGCTTGCC TGGCCTCCAG GCGGGAGTAC TTGAAGCTGG AGAAATGGCT GACCGATAAG 
ATCCGCGATC ATGGTGAGCC CTTCATGCAG GCCATGATAA AGGTGCTGCT GCGGCGCTGC 
CCACAGGTGG CCAATGCCAA GGTGCCCGAG GAACAGTTGC CACCGAAACA GGCCCAGCTG 
CTGCCCGAGA CGGTAACCAC GATGATCAAT TCCCTCCAGA CGTGCATCAA CAGTTGCATG 
CAGCCCGAGA TGGTCGAGAT GATCATGCAG ATGACCGCCA ATGTGGCCAT TATGGCGAAT 
AAGGCACGTG CCCAACAGCA GCAGCAGCAG CAACAACAAC AGCAGCCCGG TCTGGTGCCG 
CCACCGCCGC CGAGTATTCT GCGTCATCGC GGGATGGATC TACCCGGTGG CATTGTTCCC 
CCATCACCGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGC CCTTCTCCGG CAGCCTCAAT 
GCCCAGCAAA TGTTTGGTCC CGGCATAGAT CCACTCACCA ATATGTCAAA CAATCTGGCA 
GGCCTCAATC TGAGTGGACC GAATGGAGCC TTCAACTTTG GAAATATGTT AACATCTCCG 
TCCCGCCTGA TGGGTCCCGG AGGCAGCCCC TATCCTCTGA ATCCCATGCA GATGCCGCAG 
CCACTACCGC CGCCACCGAA TGTCGGAAAT CTGGGTCGCA TGCTACCCGG GGGACCGCAA 
CAGCCGCCGA CACCATCATC GGCACCGCCG ACGGCACCCA ATCCCAACAA TCCGGTGATG 
GCTGACCTGC AGATACCCGT GTCCAAGGAG GTGGAGGATG AGGTGAACTC GTATTTCCAA 
CGCATATACA ACCATCAGCC CAATCCGACG CTCTCGATTG ACGAAGTGCT GGACATACTG 
CAGCGCTTCA AGGAGTCCAG CAATCGACGG GAGCAGGAGG TCTTCCTGTG CATGCTGCGA 
AACCTGTTCG AGGAGTATCG CTTCTTCTGC CAGTATCCGG AAAAGGAGCT ACAGATCACC 
GCTCAACTGT TTGGCGGCAT CATAGACCGC AACCTGGTGC CCACTTTCGT GGCGCTCGGT 
CTCTCGCTGC GCTGCGTCCT GGATGCCCTG CGCAAGCCAG ACGGCTCCAA GCTCTATTAC 
TTTGGAGTGA CGGCCCTCGA TCGGTTCAAG ACACGACTCC ACACATACAA CAAGTACTGC 
GAGCATATCC GCTCCATTCC GCACTTCTCG GACTTTCCGC AGCACCTCAT CCAGTATGTG 
GAATATGGGA TGCACGGCCA GGAGCCCCCG CCGCATAAGC TGGTGGGCCT GAGCAACACA 
ATCTCGGCCC TAGCCACAGG ACCACCGACG GAACCGCTCT ATCGCACCAG TGCCATGCAA 
GGTCATGTGC CGGCAACTGC AGCTCCCAGC TCCGGCCAGA AGCCCACTGT GGCTGTGTCG 
CATGCCACAC GCATGAAGTC CATTGCCAAT GCGACGAATA TCGACACTCT GCTGGTGGCC 
AATCAGGAGG AGAAGGTGAC GGTGCCGCCG GAACCGGTGC AGGACAAGAC GGCCTTTATA 
TTCAACAATC TCAGTCAGCT GAACATACCG CAAAAGTGCG ATGAGATCAA GGAGATTATG 
ACCCAGGAGT ATTGGCCATG GCTGGCCCAG TATCTAGTCC TCAAGCGAGC CTCCATGGAG 
TTCAACTTTC ACACGCTCTA CTACAACTTT CTCGATGCGC TCAAGAACGG CGAGATCAAT 
CGTTTTGTGA CCAAGGAGAC ACTGCGCAAC ATCAAGGTGC TGCTGCGCTC CGACAAGGGT 
GTGATCAATT TCTCGGACCG CAGTCTGCTG AAGAATCTCG GCCACTGGCT GGGCATGATG 
ACCCTGGGTC GCAACCGACC CATTCTGCAG CTTGACCTCG ATCTCAAGTC GCTACTGGCC 
GAGGCCTATC ACAAGGGGCA GCAGGAGCTA CTGTTCGTTG TGCCTTTTGT GGCAAAGATC 
TTGGAATCGT CGGCAAAGTC GAAAATCTTC CGATCCCCCA ATCCCTGGAC AATGGGCATC 
ATGTATGTGC TGGGGGAACT GCATCAGGAG CCGGATCTCA AGTTGAACCT CAAGTTCGAA 
ATCGAAGTTC TCTGCAAAAC ACTCAATCTG GAGTTGGCCA AGTTGCGGCC AGTCATCTAC 
CTGAAGGATC CCACCCGGGC CCTGCACATC GAGCAGCAAA TGTCCCAGCC GAAGCCCAAG 
CAGACGGAAT CTGTGCCTTC GCCAGCAGCA ATCCAAGCGA TCCAACAGCC ACCGCCGCCG 
CAGCAGCAAC AACAGCAGCA GCAACAACAA CAACAACAGC AGCAGCCGCC ACCACAGCAA 
CAACAGCAGC AGCAGCAGCA ACAACAACCT CCTCCACCGC CTCCAGCGGA GGTGGATGCC 
GCCCAGGCGG CAGCCATGAT GATGAACGCT GCTGGAGGAG GCTCGAACAA CGCATCAGGA 
TCGGTTGCCT CGCCCAGTCT GCCCATCGAT CCCAGTCAGG TGTCCCTGCC ACCGCCAGAG 
CCACGCTACT CTTACGTGGA GGTCAACGTG ACCAACTTCC AGCTAATAGC CCAGCAGCTG 
GTGCTGCCAC CCAACATACC CTTCCTGCAC GCCAATCCGG GCATCAAGCA CATCGTCATA 
AATGCCGTGG AGCGCACCAT TACCGATTGG CTGCAGCCCG TGGTGGATCG CAGCATCCGC 
ATCGCCTGCG CCACCACAGA GCAGATCATA CGCAAGGACT TTGCCCTGGA TGCGGATGAG 
AACCGCATGC GCACTGCTGC CCACCAAATG GTGCGCAATC TGGCTGCCGG CATGGCCATG 
ATTACCGGCA AAGACGAGAT CGCGCGCGCC ATTAGCCAGA ATCTACACAA AGCCTTTGTG 
TCGGCTCTGA CGGGCATGCC CAGCCTCTCG GAGATTCAGG CCGCCTCCAT GCAGCTGGCC 
AGCGAGAATG TGGAGCTGGT GTGCGCCTTC ATCCAGAAGA CATCGGCCGA GAAGGCGGCC 
CTCGAGATCG ATCGACGCCT TTCCACCGAC ATCGAGACGC GGAAGATTGC CCGCGAGGAG 
GGAAGTCGCT TTGTGGATGC CCAGATCTTG AGCTATCAGC AGGAGCGACT GCCGGAGGCT 
GTGCGCATCA AGGTGGGCGC TGCCCCGGCC ACACTGTATG CGGTCTACTC GGAGTTTGCT 
CGCACCATTC CGGGATTCCA GCAGATGAGC GACAGGGACA TTGCCCTGTT CGCTCCCAAG 
CCGCAGGACC TGCCACCGCC GAATGTATTC GCCAACGATG ACAGCAGCAT GGTGTACGCC 
GAAGTGGCCG CCAAAATGGA GGCCTTTATG AACACGGCCA TTAATGTGCC GACGCTGCAG 
CTGCAGGCCG GCAAAATGCA CATGCTCCTC AACGCCCTGA TGTCCACGCG TCGTCTGCGC 
GATCAGGAGT CTGCGTTCAT TCTGCTGACC CGCGCCGTCG AGGGTCTCAC CGAGGGACTC 
ATCAACGTCC AGGAGCACTT GGAGCAGATG AAGCTTTATC GCGACATTCA CCTGCGCATC 
CTCAGTCTGC TGCACAACAG CTTTGGGGCC CCCAACACCG AGCGAGCGGT GACTAAGTGC 
TTCTTCGAAA TCCGCGAGGA AGCCCGCTTC AACGTGGAGG CCGCCCGTGC CCTGATCACT 
GCGCACTTTG TCAACCTCAA TCAGTTCGAT GGCATGCTGC GCGACTGCAT GGACAATGGC 
AACAATTACA TGGCCATCTC GTTTGGAATT GGTCTGATCG AGCGCCTCAT TGTGGAGGAT 
CGGGCCATTA ACATTGTGTC CGACAACGAG TTCATGGCCA CCGTCGAGCT GCTCGGCCGT 
CTCACCCAAC ATCGCCATCG GTATCCCGAG TGCATTGTCA ATGCCATCGA TACACTGTGG 
TCCGGCAACT TCAACTCCAC CGCTGACTAC AGTCCGTTCA ATGCCAACGA GCGTTCTCTG 
GCCGGTGCCT CCCAGTACAT ACACTCCGGC ATGCTTCACG TTAGGTCATG CGACACAGAC 
GATCCGCCAG GCTTGCAGGA GAAGACCGAG TTCTTGCTCA AGGACTGGGT GGCCCTCTAC 
GCACAGCAGA CCCAACAGAC GACGCGCGAT GCCCGGAACT TTGGGGCCTT TGTCCAGAAG 
ATGAACACCT ATGGGATACT AAAGACAGAC GATTTGATCA CACGCTTCTT CCGGCAGGCG 
ACGAACATCT GCACGGATGT GGTGTACCGC ATGTTTGCGG ATCCGAGCAT GCCCATTAAC 
CAGGCGAAGA ACAAGATATT CCAGTGGATC GATGCGTTTG TGCATCTGAT TGCGATGCTC 
GTGCGACACT CTGGCGAACC GGGTAACCCC ACAACCAAGA TCAACCTGCT GAACAAGGTG 
CTGGGCATTG TGCTGGGCAC ACTTCTCAAG GATCACGAGA TGCGTGGCGT TAGCTTCCAG 
CAGGTGGGCT ACCACCGGTT CTTCATGATG CTCTTCATGG AGCTGTGCTC GGCCGATGTC 
AACCTGGACT CGCTGATGCA CAGCATTGTG TCGGCGTTTG CCTACACATA CCACCAGCTC 
AAACCCAGTG TGGCGCCCGG CTTCTGCTTT GCCTGGCTGG AGCTCATTTC ACACCGCGTC 
TTCCTCGGCC GGATACTCGT TCAGATACCC GGCCAGAAGG GCTGGCCGCT GTACGCCCAG 
CTGCTGCAGG ACCTTTTCAA ATATCTGGCC CCCTTCTTGC GCAACACGGA GCTGGGCAAG 
CCCGTGCAGC TCCTCTACAA GGGCACCTTG CGCGTCCTGC TGGTGCTGCT GCACGACTTT 
CCAGAGTTCC TGTGCGACTA TCATTTCGGG TTCTGCGACA CCATACCGCC CAACTGTGTT 
CAGATGCGCA ACATCATCCT GTCGGCATTC CCGCGCAACA TGCGCCTGCC CGACCCCTTC 
ACGCCCAACC TCAAGGTGGA CATGCTCTCG GACAGCAGCA ATGCCCCCAA GGTGCTCAGC 
AGCTACATCG TGAACATAAA GCCGCCGAAC TTCAAGAAGG ACCTCGACTC TTACCTCAAG 
GCACGCGCCC CAGTCACCTT CCTCTCGGAA CTGCGCGGCC ATTTGCAGGT GACCAGTGAG 
CCGGGCACCC GCTACAACAT GACACTGATG AATGCCCTGG TCATGTATGT GGGCACACAG 
GCAATTGCTC TCATCAGGAA CAAAAACTTT GTGCCCAACA CTTCCAATAT TGCACACAGC 
GCGCACATGG ATATCTTCCA GAATCTGGCC GTTGACTTGG ACACCGAGGG TCGCTATCTG 
TTCCTCAATG CGATTGCCAA TCAGTTGCGG TATCCCAACA GCCACACGCA CTACTTTAGC 
TGTGCCGTCT TGCACTTGTT TGCCGAGGCA AACTCGGAGG CGATCCAGGA GCAAATCACG 
CGTGTTCTAC TCGAGCGTTT GATTGTGAAC CGGCCGCATC CGTGGGGTCT GCTCATCACC 
TTCATTGAGC TGATCAAGAA CCCCATCTAC AAGTTCTGGG ATCACGATTT TGTGCACTGT 
GCTCCGGAGA TAACCAAGCT CTTCGAATCT GTGGCTCGCT CCTGCCTGGC CAAGTCGAAC 
GTCACCCAGC AGCTGAACAT GGCCATCGTC GATGGAGAGA GCCAGGAGGT GGGCAACATT 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004444445.2
CDS	615..8300
Translation	MNVENLKTPPTQLRNLVKHVNKRNFNETSEQINQFIKDHGLEADRSSLRYLFTAINFSDPSPTVTVQLQAKLLGGQLQRQLQSSSFVSNICFAFDQHFANSQKSLKPSETAELIGQVCRLTGINKLCECVFALAVTHSSHPELRESARNCLRGSITELLDSYLGNNNSSSATSGLQDISIDLLQYLLCCLKIYVTPKLHEQFLIKLREEFPRQAVPLILAPFLYGSTTAATSNPVGANASETDAEAEATTTTETETASSSISPSTSSNSCGTDALNEVGIEDIYDHLSEIIFTNQGKNNIMDTSWINLILEIGYEFTSSVEECKNHLCSRERERTELQPKDVAKIVGLMCRRHSSLLDCNVNLPTPANFWPGQGQGGGSNSSGGSSQAQATPAQQGQNSSSNSNNNDGASDGGGSSSSDKKDKKETTDATQTWKPDVFVQALKELVPQLNWKDVCIELDHPEFVLKDRIGLDLLLTILRLATQSNIFPHPECIYRHWANTEGQLSLISTLLKNPDLFSFADFVFSQPTLDVLKTAPDADNKEISAWKSLHLVEVLLSIADKGYYTQVHELFKFPAQNCPDVLFLALLHINPPLTPLRQDLFNLLIPTFLNNHPNSNVILASAWSSTNFSLRPNIMNAMSEWYLRSSDFDQVKLSRILDLAQDLKALSSLLNARSFLFIIDLACLASRREYLKLEKWLTDKIRDHGEPFMQAMIKVLLRRCPQVANAKVPEEQLPPKQAQLLPETVTTMINSLQTCINSCMQPEMVEMIMQMTANVAIMANKARAQQQQQQQQQQQPGLVPPPPPSILRHRGMDLPGGIVPPSPQQQQQQQQQQPFSGSLNAQQMFGPGIDPLTNMSNNLAGLNLSGPNGAFNFGNMLTSPSRLMGPGGSPYPLNPMQMPQPLPPPPNVGNLGRMLPGGPQQPPTPSSAPPTAPNPNNPVMADLQIPVSKEVEDEVNSYFQRIYNHQPNPTLSIDEVLDILQRFKESSNRREQEVFLCMLRNLFEEYRFFCQYPEKELQITAQLFGGIIDRNLVPTFVALGLSLRCVLDALRKPDGSKLYYFGVTALDRFKTRLHTYNKYCEHIRSIPHFSDFPQHLIQYVEYGMHGQEPPPHKLVGLSNTISALATGPPTEPLYRTSAMQGHVPATAAPSSGQKPTVAVSHATRMKSIANATNIDTLLVANQEEKVTVPPEPVQDKTAFIFNNLSQLNIPQKCDEIKEIMTQEYWPWLAQYLVLKRASMEFNFHTLYYNFLDALKNGEINRFVTKETLRNIKVLLRSDKGVINFSDRSLLKNLGHWLGMMTLGRNRPILQLDLDLKSLLAEAYHKGQQELLFVVPFVAKILESSAKSKIFRSPNPWTMGIMYVLGELHQEPDLKLNLKFEIEVLCKTLNLELAKLRPVIYLKDPTRALHIEQQMSQPKPKQTESVPSPAAIQAIQQPPPPQQQQQQQQQQQQQQQPPPQQQQQQQQQQQPPPPPPAEVDAAQAAAMMMNAAGGGSNNASGSVASPSLPIDPSQVSLPPPEPRYSYVEVNVTNFQLIAQQLVLPPNIPFLHANPGIKHIVINAVERTITDWLQPVVDRSIRIACATTEQIIRKDFALDADENRMRTAAHQMVRNLAAGMAMITGKDEIARAISQNLHKAFVSALTGMPSLSEIQAASMQLASENVELVCAFIQKTSAEKAALEIDRRLSTDIETRKIAREEGSRFVDAQILSYQQERLPEAVRIKVGAAPATLYAVYSEFARTIPGFQQMSDRDIALFAPKPQDLPPPNVFANDDSSMVYAEVAAKMEAFMNTAINVPTLQLQAGKMHMLLNALMSTRRLRDQESAFILLTRAVEGLTEGLINVQEHLEQMKLYRDIHLRILSLLHNSFGAPNTERAVTKCFFEIREEARFNVEAARALITAHFVNLNQFDGMLRDCMDNGNNYMAISFGIGLIERLIVEDRAINIVSDNEFMATVELLGRLTQHRHRYPECIVNAIDTLWSGNFNSTADYSPFNANERSLAGASQYIHSGMLHVRSCDTDDPPGLQEKTEFLLKDWVALYAQQTQQTTRDARNFGAFVQKMNTYGILKTDDLITRFFRQATNICTDVVYRMFADPSMPINQAKNKIFQWIDAFVHLIAMLVRHSGEPGNPTTKINLLNKVLGIVLGTLLKDHEMRGVSFQQVGYHRFFMMLFMELCSADVNLDSLMHSIVSAFAYTYHQLKPSVAPGFCFAWLELISHRVFLGRILVQIPGQKGWPLYAQLLQDLFKYLAPFLRNTELGKPVQLLYKGTLRVLLVLLHDFPEFLCDYHFGFCDTIPPNCVQMRNIILSAFPRNMRLPDPFTPNLKVDMLSDSSNAPKVLSSYIVNIKPPNFKKDLDSYLKARAPVTFLSELRGHLQVTSEPGTRYNMTLMNALVMYVGTQAIALIRNKNFVPNTSNIAHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCAVLHLFAEANSEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPIYKFWDHDFVHCAPEITKLFESVARSCLAKSNVTQQLNMAIVDGESQEVGNIN

Target ORF information:

RefSeq Version	XM_004444388.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA30445), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004444388.2

ORF Insert Sequence:

ATGAACGTAG AGAACTTGAA AACACCGCCA ACGCAGCTTC GTAATTTGGT CAAACACGTG 
AACAAGCGAA ATTTTAATGA AACCAGCGAG CAAATAAACC AGTTCATCAA AGACCACGGC 
CTGGAAGCTG ATCGTAGCAG TCTGCGCTAT CTGTTCACTG CGATCAACTT CAGTGACCCA 
TCGCCGACCG TCACCGTCCA GTTGCAAGCC AAATTACTCG GTGGCCAGTT GCAGCGACAG 
TTGCAAAGCT CCTCGTTTGT GTCCAACATC TGCTTCGCGT TTGACCAGCA CTTTGCCAAC 
AGCCAAAAGT CTCTCAAGCC GTCTGAGACA GCAGAGCTCA TTGGCCAGGT GTGCAGACTG 
ACCGGCATAA ACAAGCTCTG CGAGTGCGTA TTTGCCTTGG CGGTGACCCA CTCATCGCAT 
CCAGAGCTTC GAGAATCGGC CAGAAACTGC TTGCGAGGCT CCATAACCGA GCTGCTCGAT 
TCCTATTTGG GGAACAACAA CAGCTCCTCA GCTACCAGTG GGCTGCAAGA TATATCGATC 
GATCTTCTGC AGTATTTGCT GTGCTGCCTC AAAATTTACG TGACGCCGAA GCTGCACGAG 
CAATTTTTGA TCAAACTACG TGAGGAGTTT CCGCGGCAGG CGGTGCCTTT GATTTTGGCG 
CCATTCCTTT ACGGCTCAAC GACGGCGGCG ACCTCGAATC CAGTTGGGGC CAACGCCAGC 
GAAACTGATG CAGAGGCTGA GGCAACGACA ACGACAGAGA CAGAGACGGC CAGCAGCAGC 
ATCAGCCCCA GCACCAGCAG CAACAGTTGC GGCACGGATG CCTTGAATGA GGTTGGAATC 
GAGGATATCT ATGACCATTT AAGCGAAATA ATATTCACAA ACCAGGGTAA AAATAATATT 
ATGGACACAT CTTGGATTAA TCTAATCCTT GAAATCGGTT ACGAATTTAC ATCGAGCGTC 
GAAGAGTGTA AAAATCACCT GTGCTCTCGC GAACGAGAGC GCACCGAACT CCAGCCAAAA 
GATGTTGCCA AAATCGTTGG TCTCATGTGC CGGCGGCACT CGTCTTTGCT CGATTGTAAT 
GTTAATCTAC CGACGCCAGC TAATTTCTGG CCTGGACAAG GTCAAGGCGG TGGCAGCAAC 
AGTAGCGGGG GCTCGTCACA AGCACAGGCC ACCCCAGCGC AGCAGGGACA GAACTCAAGC 
AGCAACAGTA ACAACAACGA TGGAGCCAGC GACGGTGGAG GCAGCTCGTC CAGTGATAAG 
AAGGACAAGA AGGAGACGAC AGATGCCACA CAGACATGGA AGCCGGATGT CTTTGTGCAG 
GCACTCAAGG AACTGGTGCC ACAGCTCAAC TGGAAGGACG TTTGCATTGA GCTCGATCAT 
CCCGAGTTTG TGCTGAAGGA TCGCATCGGC TTGGACCTGC TGTTGACAAT TCTGCGACTG 
GCCACACAGT CGAACATCTT TCCACATCCA GAATGCATTT ACCGCCACTG GGCCAACACG 
GAGGGTCAGC TGTCGCTGAT CTCTACGCTG CTAAAGAATC CGGATCTGTT CTCCTTTGCC 
GACTTTGTGT TCAGTCAGCC AACGCTGGAT GTCCTGAAGA CGGCACCGGA TGCGGACAAT 
AAGGAGATTT CGGCATGGAA GTCCCTCCAC CTTGTCGAGG TCCTGCTCTC TATTGCGGAC 
AAGGGCTACT ACACGCAGGT CCATGAGCTA TTCAAGTTCC CTGCACAGAA CTGCCCGGAC 
GTCCTGTTTT TGGCACTGCT GCACATAAAT CCACCACTGA CACCGCTACG GCAGGACTTG 
TTCAATTTGC TGATACCGAC GTTCCTCAAC AATCATCCCA ATTCGAATGT GATCCTGGCC 
AGCGCCTGGA GCTCGACCAA CTTCTCGCTG CGGCCGAACA TTATGAACGC CATGTCCGAG 
TGGTATCTGC GCAGCAGCGA CTTTGATCAA GTAAAGCTGT CGCGCATTCT CGATCTCGCG 
CAGGACCTGA AGGCCCTCTC GTCGCTCCTG AATGCACGCT CCTTTCTGTT CATCATCGAT 
CTGGCTTGCC TGGCCTCCAG GCGGGAGTAC TTGAAGCTGG AGAAATGGCT GACCGATAAG 
ATCCGCGATC ATGGTGAGCC CTTCATGCAG GCCATGATAA AGGTGCTGCT GCGGCGCTGC 
CCACAGGTGG CCAATGCCAA GGTGCCCGAG GAACAGTTGC CACCGAAACA GGCCCAGCTG 
CTGCCCGAGA CGGTAACCAC GATGATCAAT TCCCTCCAGA CGTGCATCAA CAGTTGCATG 
CAGCCCGAGA TGGTCGAGAT GATCATGCAG ATGACCGCCA ATGTGGCCAT TATGGCGAAT 
AAGGCACGTG CCCAACAGCA GCAGCAGCAG CAACAACAAC AGCAGCCCGG TCTGGTGCCG 
CCACCGCCGC CGAGTATTCT GCGTCATCGC GGGATGGATC TACCCGGTGG CATTGTTCCC 
CCATCACCGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGC CCTTCTCCGG CAGCCTCAAT 
GCCCAGCAAA TGTTTGGTCC CGGCATAGAT CCACTCACCA ATATGTCAAA CAATCTGGCA 
GGCCTCAATC TGAGTGGACC GAATGGAGCC TTCAACTTTG GAAATATGTT AACATCTCCG 
TCCCGCCTGA TGGGTCCCGG AGGCAGCCCC TATCCTCTGA ATCCCATGCA GATGCCGCAG 
CCACTACCGC CGCCACCGAA TGTCGGAAAT CTGGGTCGCA TGCTACCCGG GGGACCGCAA 
CAGCCGCCGA CACCATCATC GGCACCGCCG ACGGCACCCA ATCCCAACAA TCCGGTGATG 
GCTGACCTGC AGATACCCGT GTCCAAGGAG GTGGAGGATG AGGTGAACTC GTATTTCCAA 
CGCATATACA ACCATCAGCC CAATCCGACG CTCTCGATTG ACGAAGTGCT GGACATACTG 
CAGCGCTTCA AGGAGTCCAG CAATCGACGG GAGCAGGAGG TCTTCCTGTG CATGCTGCGA 
AACCTGTTCG AGGAGTATCG CTTCTTCTGC CAGTATCCGG AAAAGGAGCT ACAGATCACC 
GCTCAACTGT TTGGCGGCAT CATAGACCGC AACCTGGTGC CCACTTTCGT GGCGCTCGGT 
CTCTCGCTGC GCTGCGTCCT GGATGCCCTG CGCAAGCCAG ACGGCTCCAA GCTCTATTAC 
TTTGGAGTGA CGGCCCTCGA TCGGTTCAAG ACACGACTCC ACACATACAA CAAGTACTGC 
GAGCATATCC GCTCCATTCC GCACTTCTCG GACTTTCCGC AGCACCTCAT CCAGTATGTG 
GAATATGGGA TGCACGGCCA GGAGCCCCCG CCGCATAAGC TGGTGGGCCT GAGCAACACA 
ATCTCGGCCC TAGCCACAGG ACCACCGACG GAACCGCTCT ATCGCACCAG TGCCATGCAA 
GGTCATGTGC CGGCAACTGC AGCTCCCAGC TCCGGCCAGA AGCCCACTGT GGCTGTGTCG 
CATGCCACAC GCATGAAGTC CATTGCCAAT GCGACGAATA TCGACACTCT GCTGGTGGCC 
AATCAGGAGG AGAAGGTGAC GGTGCCGCCG GAACCGGTGC AGGACAAGAC GGCCTTTATA 
TTCAACAATC TCAGTCAGCT GAACATACCG CAAAAGTGCG ATGAGATCAA GGAGATTATG 
ACCCAGGAGT ATTGGCCATG GCTGGCCCAG TATCTAGTCC TCAAGCGAGC CTCCATGGAG 
TTCAACTTTC ACACGCTCTA CTACAACTTT CTCGATGCGC TCAAGAACGG CGAGATCAAT 
CGTTTTGTGA CCAAGGAGAC ACTGCGCAAC ATCAAGGTGC TGCTGCGCTC CGACAAGGGT 
GTGATCAATT TCTCGGACCG CAGTCTGCTG AAGAATCTCG GCCACTGGCT GGGCATGATG 
ACCCTGGGTC GCAACCGACC CATTCTGCAG CTTGACCTCG ATCTCAAGTC GCTACTGGCC 
GAGGCCTATC ACAAGGGGCA GCAGGAGCTA CTGTTCGTTG TGCCTTTTGT GGCAAAGATC 
TTGGAATCGT CGGCAAAGTC GAAAATCTTC CGATCCCCCA ATCCCTGGAC AATGGGCATC 
ATGTATGTGC TGGGGGAACT GCATCAGGAG CCGGATCTCA AGTTGAACCT CAAGTTCGAA 
ATCGAAGTTC TCTGCAAAAC ACTCAATCTG GAGTTGGCCA AGTTGCGGCC AGTCATCTAC 
CTGAAGGATC CCACCCGGGC CCTGCACATC GAGCAGCAAA TGTCCCAGCC GAAGCCCAAG 
CAGACGGAAT CTGTGCCTTC GCCAGCAGCA ATCCAAGCGA TCCAACAGCC ACCGCCGCCG 
CAGCAGCAAC AACAGCAGCA GCAACAACAA CAACAACAGC AGCAGCCGCC ACCACAGCAA 
CAACAGCAGC AGCAGCAGCA ACAACAACCT CCTCCACCGC CTCCAGCGGA GGTGGATGCC 
GCCCAGGCGG CAGCCATGAT GATGAACGCT GCTGGAGGAG GCTCGAACAA CGCATCAGGA 
TCGGTTGCCT CGCCCAGTCT GCCCATCGAT CCCAGTCAGG TGTCCCTGCC ACCGCCAGAG 
CCACGCTACT CTTACGTGGA GGTCAACGTG ACCAACTTCC AGCTAATAGC CCAGCAGCTG 
GTGCTGCCAC CCAACATACC CTTCCTGCAC GCCAATCCGG GCATCAAGCA CATCGTCATA 
AATGCCGTGG AGCGCACCAT TACCGATTGG CTGCAGCCCG TGGTGGATCG CAGCATCCGC 
ATCGCCTGCG CCACCACAGA GCAGATCATA CGCAAGGACT TTGCCCTGGA TGCGGATGAG 
AACCGCATGC GCACTGCTGC CCACCAAATG GTGCGCAATC TGGCTGCCGG CATGGCCATG 
ATTACCGGCA AAGACGAGAT CGCGCGCGCC ATTAGCCAGA ATCTACACAA AGCCTTTGTG 
TCGGCTCTGA CGGGCATGCC CAGCCTCTCG GAGATTCAGG CCGCCTCCAT GCAGCTGGCC 
AGCGAGAATG TGGAGCTGGT GTGCGCCTTC ATCCAGAAGA CATCGGCCGA GAAGGCGGCC 
CTCGAGATCG ATCGACGCCT TTCCACCGAC ATCGAGACGC GGAAGATTGC CCGCGAGGAG 
GGAAGTCGCT TTGTGGATGC CCAGATCTTG AGCTATCAGC AGGAGCGACT GCCGGAGGCT 
GTGCGCATCA AGGTGGGCGC TGCCCCGGCC ACACTGTATG CGGTCTACTC GGAGTTTGCT 
CGCACCATTC CGGGATTCCA GCAGATGAGC GACAGGGACA TTGCCCTGTT CGCTCCCAAG 
CCGCAGGACC TGCCACCGCC GAATGTATTC GCCAACGATG ACAGCAGCAT GGTGTACGCC 
GAAGTGGCCG CCAAAATGGA GGCCTTTATG AACACGGCCA TTAATGTGCC GACGCTGCAG 
CTGCAGGCCG GCAAAATGCA CATGCTCCTC AACGCCCTGA TGTCCACGCG TCGTCTGCGC 
GATCAGGAGT CTGCGTTCAT TCTGCTGACC CGCGCCGTCG AGGGTCTCAC CGAGGGACTC 
ATCAACGTCC AGGAGCACTT GGAGCAGATG AAGCTTTATC GCGACATTCA CCTGCGCATC 
CTCAGTCTGC TGCACAACAG CTTTGGGGCC CCCAACACCG AGCGAGCGGT GACTAAGTGC 
TTCTTCGAAA TCCGCGAGGA AGCCCGCTTC AACGTGGAGG CCGCCCGTGC CCTGATCACT 
GCGCACTTTG TCAACCTCAA TCAGTTCGAT GGCATGCTGC GCGACTGCAT GGACAATGGC 
AACAATTACA TGGCCATCTC GTTTGGAATT GGTCTGATCG AGCGCCTCAT TGTGGAGGAT 
CGGGCCATTA ACATTGTGTC CGACAACGAG TTCATGGCCA CCGTCGAGCT GCTCGGCCGT 
CTCACCCAAC ATCGCCATCG GTATCCCGAG TGCATTGTCA ATGCCATCGA TACACTGTGG 
TCCGGCAACT TCAACTCCAC CGCTGACTAC AGTCCGTTCA ATGCCAACGA GCGTTCTCTG 
GCCGGTGCCT CCCAGTACAT ACACTCCGGC ATGCTTCACG TTAGGTCATG CGACACAGAC 
GATCCGCCAG GCTTGCAGGA GAAGACCGAG TTCTTGCTCA AGGACTGGGT GGCCCTCTAC 
GCACAGCAGA CCCAACAGAC GACGCGCGAT GCCCGGAACT TTGGGGCCTT TGTCCAGAAG 
ATGAACACCT ATGGGATACT AAAGACAGAC GATTTGATCA CACGCTTCTT CCGGCAGGCG 
ACGAACATCT GCACGGATGT GGTGTACCGC ATGTTTGCGG ATCCGAGCAT GCCCATTAAC 
CAGGCGAAGA ACAAGATATT CCAGTGGATC GATGCGTTTG TGCATCTGAT TGCGATGCTC 
GTGCGACACT CTGGCGAACC GGGTAACCCC ACAACCAAGA TCAACCTGCT GAACAAGGTG 
CTGGGCATTG TGCTGGGCAC ACTTCTCAAG GATCACGAGA TGCGTGGCGT TAGCTTCCAG 
CAGGTGGGCT ACCACCGGTT CTTCATGATG CTCTTCATGG AGCTGTGCTC GGCCGATGTC 
AACCTGGACT CGCTGATGCA CAGCATTGTG TCGGCGTTTG CCTACACATA CCACCAGCTC 
AAACCCAGTG TGGCGCCCGG CTTCTGCTTT GCCTGGCTGG AGCTCATTTC ACACCGCGTC 
TTCCTCGGCC GGATACTCGT TCAGATACCC GGCCAGAAGG GCTGGCCGCT GTACGCCCAG 
CTGCTGCAGG ACCTTTTCAA ATATCTGGCC CCCTTCTTGC GCAACACGGA GCTGGGCAAG 
CCCGTGCAGC TCCTCTACAA GGGCACCTTG CGCGTCCTGC TGGTGCTGCT GCACGACTTT 
CCAGAGTTCC TGTGCGACTA TCATTTCGGG TTCTGCGACA CCATACCGCC CAACTGTGTT 
CAGATGCGCA ACATCATCCT GTCGGCATTC CCGCGCAACA TGCGCCTGCC CGACCCCTTC 
ACGCCCAACC TCAAGGTGGA CATGCTCTCG GACAGCAGCA ATGCCCCCAA GGTGCTCAGC 
AGCTACATCG TGAACATAAA GCCGCCGAAC TTCAAGAAGG ACCTCGACTC TTACCTCAAG 
GCACGCGCCC CAGTCACCTT CCTCTCGGAA CTGCGCGGCC ATTTGCAGGT GACCAGTGAG 
CCGGGCACCC GCTACAACAT GACACTGATG AATGCCCTGG TCATGTATGT GGGCACACAG 
GCAATTGCTC TCATCAGGAA CAAAAACTTT GTGCCCAACA CTTCCAATAT TGCACACAGC 
GCGCACATGG ATATCTTCCA GAATCTGGCC GTTGACTTGG ACACCGAGGG TCGCTATCTG 
TTCCTCAATG CGATTGCCAA TCAGTTGCGG TATCCCAACA GCCACACGCA CTACTTTAGC 
TGTGCCGTCT TGCACTTGTT TGCCGAGGCA AACTCGGAGG CGATCCAGGA GCAAATCACG 
CGTGTTCTAC TCGAGCGTTT GATTGTGAAC CGGCCGCATC CGTGGGGTCT GCTCATCACC 
TTCATTGAGC TGATCAAGAA CCCCATCTAC AAGTTCTGGG ATCACGATTT TGTGCACTGT 
GCTCCGGAGA TAACCAAGCT CTTCGAATCT GTGGCTCGCT CCTGCCTGGC CAAGTCGAAC 
GTCACCCAGC AGCTGAACAT GGCCATCGTC GATGGAGAGA GCCAGGAGGT GGGCAACATT 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

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Dpse\GA30445 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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