Dpse\GA21135 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA21135
Entrez Gene ID	4805482
Full Name	GA21135 gene product from transcript GA21135-RA
Synonyms	GA21135,dpse_GLEANR_16315
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001361840.3	XP_001361877.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA21135 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA21135 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe04582	XM_001361840.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA21135), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA21135 ( XM_001361840.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001361877.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA21135), mRNA.

Price & Availability↑

CloneID	ODe04582
Clone ID Related Accession (Same CDS sequence)	XM_001361840.3
Accession Version	XM_001361840.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_001361840.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCGCCT CGAACGGCGT CATCGTCAAG GACGACGATG ACAAATCGCG TTTGCAGTAC 
AAAACGAACA TCGTCCTGGG ATCAGCAGCA TTCGAAGACC AGGACAAAGA CACAAAGAGC 
TTTGCTCCCA ACAAGACGAA AAAAAAGAAT TCTAAGCATG ACGAGGCGGA AGAGGAGGAG 
CCAAACGATG GCTCCTACAA AGAGGAGGTC AAACAGGTTG GCTACTTCCA GATGTTCCGC 
TATGCCACCA CCAAGGACCG CATGCTCTAT GTCATCGGAC TTCTGTCCGC CGTAGCCACT 
GGTCTCACGA CTCCCGCCAA CAGCTTAATC TTCGGCAACC TGGCAAACAA CTTTATCGAT 
TTGACCGGGG TAGATGAGGG TAGGACGTAC CAACGTGACG GTGACGACGA GGGAGACTTG 
CTCCTCGACA AGGTGCGGGA GTTCTCTCTG CAGAACACAT ACATTGGAAT TGTGATGCTG 
GTGTGCTCCT ATCTCTCCAT CACCTGCTTC AATTACGCCG CCCACTCGCA GATTCTGACC 
ATCCGCTCCA AGTTCTTCCG CTCCATCCTG CACCAGGACA TGAGCTGGTA TGACTTCAAC 
CAGAGCGGCG AAGTGGCCAG CCGTATGAAC GAGGATCTGT CCAAAATGGA AGATGGTCTC 
GCCGAAAAAG TCGTCATGTT CGTCCACTAC TTTGTGTCCT TTGTGGGATC CCTGGTGCTG 
GCCTTTGTCA AAGGCTGGCA GCTGTCTCTG GTGTGCCTTA CGAGCCTGCC GCTAACTTTC 
GTCGCGATGG GACTGGTGTC GGTGGCTACC TCCAGGCTGG CCAAGCAAGA GGTTACCCAG 
TACGCTGCAG CAGCAGTCGT GGCCGAGGGC GCCCTGTCCG GGATTCGCAC GGTAAAGGCG 
TTCGAGGGAG AGGAGAAGGA GGTGTCCGCC TACAAGGAAC GCGTTGTGGC CGCCAAGCTG 
TTGAACATCA AACGCAACAT GTTCTCCGGC ATCGGCTTCG GCATGCTCTG GTTCTTCATC 
TATGCCTCGT ACGCTTTGGC CTTCTGGTAC GGCGTGGGGC TGGTAATCAA GGGGTACCAC 
GATCCGTATT ATGCCAGCTA CGACGCCGGC ACGATGATCA CCGTATTCTT CTCGGTGATG 
ATGGGTTCGA TGAACATTGG CATGGCCGCG CCCTACATCG AGGCATTCGG CATTGCCAAG 
GGCGCCTGCG CGAAGGTCTT CCACATCATC GAGCAGATCC CCACCATCAA TCCCATCGGC 
CATCAAGGTA AGAACCTCAA CGAGCCCCTC ACGACCATCG AGTTTCGTGA CGTCGAGTTC 
CAGTATCCGA CACGCAGCGA GATTCCCATT TTGAACCGTC TCAACCTCAA GATCCATCGC 
GGCCAGACGG TGGCCCTGGT CGGACCCTCC GGCTGCGGCA AGTCCACCTG CATCCAGCTG 
CTGCAGCGCT TCTACGACCC CGCCGGCGGC GATCTATTCT TCAACGGCAC CAGCCTGCGA 
GACATCGACA TCAACTGGCT GCGCTCCCGC ATTGGAGTCG TCGGTCAGGA GCCCGTGCTC 
TTTGCTACAT CGATCTACGA GAACATCCGC TACGGACGCG AGGACGCCAC TCGGGCTGAC 
ATCGAGGCAG CAGCCGAAGC CGCCAATGCG GCCGTGTTCA TCAAAAAGCT GCCACGCGGC 
TATGATACGC TGGTGGGCGA ACGCGGAGCT CAGTTGTCCG GAGGCCAGAA GCAGCGAATT 
GCCATTGCAC GGGCCCTGAT TCGCGACCCG GAGATACTGC TGTTGGACGA GGCCACCTCG 
GCCCTGGACA CTGCCAGTGA GGCGAAGGTG CAGGCTGCCC TGGAGAAGGT CAGCGCCGGA 
CGCACGACCG TCATTGTGGC CCATCGGCTG TCGACGGTAC GCCGGGCGGA CCGCATCGTC 
GTGATCAATA AAGGCGAGGT GGTGGAGAGC GGTACGCACC ATGAGCTGAT GATGCTGAAG 
AGCCACTACT TTAATCTGGT TACCACCCAG CTGGGTGAGG ATGACGGTAC CGTACTCAGC 
CCCAGCGGCG ACATTTACAA GAACTTCGAC ATCAAGGACG AGGACGAGGA GGAGATCAAG 
GTGCTGGAGG AGGACGATGA GAAGGAGCTA GAGGCCGTCG CGAAGGACAA GAAGAAGAAG 
AAGAAGGTCA AGGACCCCAA CGAGGTCAAG CCCATGGCGG AGGTGATGAA GATGAACAAG 
CCGGAGTGGG CGCAGGTTAC CGTCGGCTGC ATCTCTTCGG TGATCATGGG CTGTGCCATG 
CCCATCTTTG CCGTACTCTT CGGCAGCATC CTGCAGGTGC TCTCAGTCAA GAACAACGAC 
GAGTACGTCC GTGAGAACAG CAACAAGTAC AGTCTTTACT TCTTGATTGC CGGCATTGTT 
GTGGGTATCG CTACCTTCAT GCAGATCTAC TTCTTTGGCA TCGCCGGAGA GCGGCTCACG 
GAGCGCCTGC GCGGCCTCAT GTTCGAGAGG ATGCTGAAAC AGGAAGTGGC CTGGTTCGAT 
GACAAGGCGA ACGGCACAGG GAGTCTGTGC GCCCGCCTCT CCGGCGATGC CGCCGCTGTC 
CAAGGCGCAA CTGGCCAGAG AATCGGCACG ATCATTCAGT CTGTTTCCAC GCTAGCCCTC 
GGTATCGGTC TGTCCATGTA CTACGAGTGG AGTCTGGGTC TGGTCGCCCT CGCCTTCACG 
CCCTTTATCC TCATCGCATT TTACATGCAG CGCACGCTCA TGGCCGAAGA AAACATGGGC 
ACGGCCAAGA CCATGGAGAA CTGCACCAAG CTGGCCGTCG AGGTAGTCTC CAACATCCGC 
ACCGTCGTCT CCCTGGGGCG CGAGGAGATG TTCCACCAGA CATACATCGG CATGCTCATC 
CCGTCCGTAA ATAAAGCGAA GAAAAACACC CACTTCCGCG GCCTGGTCTA CGGCCTCGCT 
CGCTCCCTGA TGTTCTTTGC GTATGCCGCC TGCATGTACT ACGGCACCTG GTGCGTGATA 
AATCGGGGCA TCATATTCGG AGATGTCTTC AAAGTATCCC AGGCTTTGAT CATGGGAACG 
GCTTCGATTG CCAACGCCCT GGCCTTTGCC CCCAACATGC AGAAGGGAAT CACAGCCGCA 
AAGACGATCT TCACCTTCCT GCGGCGCCAG CCCATGATCG TGGACCGGCC GGGAGTGTCG 
CGCGAACCGT GGCACTGCCA GGGCAATGTC ACTTACGACA AGGTTGAGTT TAGCTATCCC 
ACGCGCAGAG AGATCCAGGT GCTCAAGGGT CTGGAACTGG GAGTGAAAAA GGGCCAGAAG 
GTGGCCCTGG TGGGTCCCTC CGGGTGCGGC AAGTCCACCT GCATCCAGCT GATCCAGCGC 
TTCTATGACG TGGACGAGGG TGCCGCCCTC ATCGATGAGC AGGATGTCCG AGACGTATCG 
ATGTCAAATC TGCGCCAGCA GCTCGGCATC GTGTCCCAGG AGCCGATCCT TTTTGACCGC 
ACCATCCGGC AGAACATTGC ATATGGCGAC AACACGAGGT CTGTCACCGA CCAGGAGATC 
ATGACAGCCT GCATGAAGTC TAATATTCAC GAGTTCATCG CGAACCTGCC GCTGGGTTAC 
GACACGAGAA TGGGTGAGAA GGGAGCCCAG CTGTCCGGCG GTCAGAAGCA GCGCATTGCC 
ATCGCGCGCG CCCTCATCCG GAACCCCAAG ATCATGCTGC TCGACGAAGC CACCTCGGCA 
CTGGACGCCG AGAGTGAAAA GGTTGTCCAA GATGCTCTGG ATGCCGCATC CGAGGGTCGC 
ACAACGATCA GCATAGCTCA CAGACTGTCC ACAGTCGTCC ATTCGGATGT GATCTTTGTG 
TTTGAGAACG GCGTAGTCTG CGAGACCGGC TCCCACAAGG ATTTGCTGGA GAATCGCGGC 
CTCTACTACA CGCTCTACAA GCTGCAGAGC GGGGCCATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001361877.2
CDS	96..4037
Translation	MTASNGVIVKDDDDKSRLQYKTNIVLGSAAFEDQDKDTKSFAPNKTKKKNSKHDEAEEEEPNDGSYKEEVKQVGYFQMFRYATTKDRMLYVIGLLSAVATGLTTPANSLIFGNLANNFIDLTGVDEGRTYQRDGDDEGDLLLDKVREFSLQNTYIGIVMLVCSYLSITCFNYAAHSQILTIRSKFFRSILHQDMSWYDFNQSGEVASRMNEDLSKMEDGLAEKVVMFVHYFVSFVGSLVLAFVKGWQLSLVCLTSLPLTFVAMGLVSVATSRLAKQEVTQYAAAAVVAEGALSGIRTVKAFEGEEKEVSAYKERVVAAKLLNIKRNMFSGIGFGMLWFFIYASYALAFWYGVGLVIKGYHDPYYASYDAGTMITVFFSVMMGSMNIGMAAPYIEAFGIAKGACAKVFHIIEQIPTINPIGHQGKNLNEPLTTIEFRDVEFQYPTRSEIPILNRLNLKIHRGQTVALVGPSGCGKSTCIQLLQRFYDPAGGDLFFNGTSLRDIDINWLRSRIGVVGQEPVLFATSIYENIRYGREDATRADIEAAAEAANAAVFIKKLPRGYDTLVGERGAQLSGGQKQRIAIARALIRDPEILLLDEATSALDTASEAKVQAALEKVSAGRTTVIVAHRLSTVRRADRIVVINKGEVVESGTHHELMMLKSHYFNLVTTQLGEDDGTVLSPSGDIYKNFDIKDEDEEEIKVLEEDDEKELEAVAKDKKKKKKVKDPNEVKPMAEVMKMNKPEWAQVTVGCISSVIMGCAMPIFAVLFGSILQVLSVKNNDEYVRENSNKYSLYFLIAGIVVGIATFMQIYFFGIAGERLTERLRGLMFERMLKQEVAWFDDKANGTGSLCARLSGDAAAVQGATGQRIGTIIQSVSTLALGIGLSMYYEWSLGLVALAFTPFILIAFYMQRTLMAEENMGTAKTMENCTKLAVEVVSNIRTVVSLGREEMFHQTYIGMLIPSVNKAKKNTHFRGLVYGLARSLMFFAYAACMYYGTWCVINRGIIFGDVFKVSQALIMGTASIANALAFAPNMQKGITAAKTIFTFLRRQPMIVDRPGVSREPWHCQGNVTYDKVEFSYPTRREIQVLKGLELGVKKGQKVALVGPSGCGKSTCIQLIQRFYDVDEGAALIDEQDVRDVSMSNLRQQLGIVSQEPILFDRTIRQNIAYGDNTRSVTDQEIMTACMKSNIHEFIANLPLGYDTRMGEKGAQLSGGQKQRIAIARALIRNPKIMLLDEATSALDAESEKVVQDALDAASEGRTTISIAHRLSTVVHSDVIFVFENGVVCETGSHKDLLENRGLYYTLYKLQSGAM

Target ORF information:

RefSeq Version	XM_001361840.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA21135), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001361840.3

ORF Insert Sequence:

ATGACCGCCT CGAACGGCGT CATCGTCAAG GACGACGATG ACAAATCGCG TTTGCAGTAC 
AAAACGAACA TCGTCCTGGG ATCAGCAGCA TTCGAAGACC AGGACAAAGA CACAAAGAGC 
TTTGCTCCCA ACAAGACGAA AAAAAAGAAT TCTAAGCATG ACGAGGCGGA AGAGGAGGAG 
CCAAACGATG GCTCCTACAA AGAGGAGGTC AAACAGGTTG GCTACTTCCA GATGTTCCGC 
TATGCCACCA CCAAGGACCG CATGCTCTAT GTCATCGGAC TTCTGTCCGC CGTAGCCACT 
GGTCTCACGA CTCCCGCCAA CAGCTTAATC TTCGGCAACC TGGCAAACAA CTTTATCGAT 
TTGACCGGGG TAGATGAGGG TAGGACGTAC CAACGTGACG GTGACGACGA GGGAGACTTG 
CTCCTCGACA AGGTGCGGGA GTTCTCTCTG CAGAACACAT ACATTGGAAT TGTGATGCTG 
GTGTGCTCCT ATCTCTCCAT CACCTGCTTC AATTACGCCG CCCACTCGCA GATTCTGACC 
ATCCGCTCCA AGTTCTTCCG CTCCATCCTG CACCAGGACA TGAGCTGGTA TGACTTCAAC 
CAGAGCGGCG AAGTGGCCAG CCGTATGAAC GAGGATCTGT CCAAAATGGA AGATGGTCTC 
GCCGAAAAAG TCGTCATGTT CGTCCACTAC TTTGTGTCCT TTGTGGGATC CCTGGTGCTG 
GCCTTTGTCA AAGGCTGGCA GCTGTCTCTG GTGTGCCTTA CGAGCCTGCC GCTAACTTTC 
GTCGCGATGG GACTGGTGTC GGTGGCTACC TCCAGGCTGG CCAAGCAAGA GGTTACCCAG 
TACGCTGCAG CAGCAGTCGT GGCCGAGGGC GCCCTGTCCG GGATTCGCAC GGTAAAGGCG 
TTCGAGGGAG AGGAGAAGGA GGTGTCCGCC TACAAGGAAC GCGTTGTGGC CGCCAAGCTG 
TTGAACATCA AACGCAACAT GTTCTCCGGC ATCGGCTTCG GCATGCTCTG GTTCTTCATC 
TATGCCTCGT ACGCTTTGGC CTTCTGGTAC GGCGTGGGGC TGGTAATCAA GGGGTACCAC 
GATCCGTATT ATGCCAGCTA CGACGCCGGC ACGATGATCA CCGTATTCTT CTCGGTGATG 
ATGGGTTCGA TGAACATTGG CATGGCCGCG CCCTACATCG AGGCATTCGG CATTGCCAAG 
GGCGCCTGCG CGAAGGTCTT CCACATCATC GAGCAGATCC CCACCATCAA TCCCATCGGC 
CATCAAGGTA AGAACCTCAA CGAGCCCCTC ACGACCATCG AGTTTCGTGA CGTCGAGTTC 
CAGTATCCGA CACGCAGCGA GATTCCCATT TTGAACCGTC TCAACCTCAA GATCCATCGC 
GGCCAGACGG TGGCCCTGGT CGGACCCTCC GGCTGCGGCA AGTCCACCTG CATCCAGCTG 
CTGCAGCGCT TCTACGACCC CGCCGGCGGC GATCTATTCT TCAACGGCAC CAGCCTGCGA 
GACATCGACA TCAACTGGCT GCGCTCCCGC ATTGGAGTCG TCGGTCAGGA GCCCGTGCTC 
TTTGCTACAT CGATCTACGA GAACATCCGC TACGGACGCG AGGACGCCAC TCGGGCTGAC 
ATCGAGGCAG CAGCCGAAGC CGCCAATGCG GCCGTGTTCA TCAAAAAGCT GCCACGCGGC 
TATGATACGC TGGTGGGCGA ACGCGGAGCT CAGTTGTCCG GAGGCCAGAA GCAGCGAATT 
GCCATTGCAC GGGCCCTGAT TCGCGACCCG GAGATACTGC TGTTGGACGA GGCCACCTCG 
GCCCTGGACA CTGCCAGTGA GGCGAAGGTG CAGGCTGCCC TGGAGAAGGT CAGCGCCGGA 
CGCACGACCG TCATTGTGGC CCATCGGCTG TCGACGGTAC GCCGGGCGGA CCGCATCGTC 
GTGATCAATA AAGGCGAGGT GGTGGAGAGC GGTACGCACC ATGAGCTGAT GATGCTGAAG 
AGCCACTACT TTAATCTGGT TACCACCCAG CTGGGTGAGG ATGACGGTAC CGTACTCAGC 
CCCAGCGGCG ACATTTACAA GAACTTCGAC ATCAAGGACG AGGACGAGGA GGAGATCAAG 
GTGCTGGAGG AGGACGATGA GAAGGAGCTA GAGGCCGTCG CGAAGGACAA GAAGAAGAAG 
AAGAAGGTCA AGGACCCCAA CGAGGTCAAG CCCATGGCGG AGGTGATGAA GATGAACAAG 
CCGGAGTGGG CGCAGGTTAC CGTCGGCTGC ATCTCTTCGG TGATCATGGG CTGTGCCATG 
CCCATCTTTG CCGTACTCTT CGGCAGCATC CTGCAGGTGC TCTCAGTCAA GAACAACGAC 
GAGTACGTCC GTGAGAACAG CAACAAGTAC AGTCTTTACT TCTTGATTGC CGGCATTGTT 
GTGGGTATCG CTACCTTCAT GCAGATCTAC TTCTTTGGCA TCGCCGGAGA GCGGCTCACG 
GAGCGCCTGC GCGGCCTCAT GTTCGAGAGG ATGCTGAAAC AGGAAGTGGC CTGGTTCGAT 
GACAAGGCGA ACGGCACAGG GAGTCTGTGC GCCCGCCTCT CCGGCGATGC CGCCGCTGTC 
CAAGGCGCAA CTGGCCAGAG AATCGGCACG ATCATTCAGT CTGTTTCCAC GCTAGCCCTC 
GGTATCGGTC TGTCCATGTA CTACGAGTGG AGTCTGGGTC TGGTCGCCCT CGCCTTCACG 
CCCTTTATCC TCATCGCATT TTACATGCAG CGCACGCTCA TGGCCGAAGA AAACATGGGC 
ACGGCCAAGA CCATGGAGAA CTGCACCAAG CTGGCCGTCG AGGTAGTCTC CAACATCCGC 
ACCGTCGTCT CCCTGGGGCG CGAGGAGATG TTCCACCAGA CATACATCGG CATGCTCATC 
CCGTCCGTAA ATAAAGCGAA GAAAAACACC CACTTCCGCG GCCTGGTCTA CGGCCTCGCT 
CGCTCCCTGA TGTTCTTTGC GTATGCCGCC TGCATGTACT ACGGCACCTG GTGCGTGATA 
AATCGGGGCA TCATATTCGG AGATGTCTTC AAAGTATCCC AGGCTTTGAT CATGGGAACG 
GCTTCGATTG CCAACGCCCT GGCCTTTGCC CCCAACATGC AGAAGGGAAT CACAGCCGCA 
AAGACGATCT TCACCTTCCT GCGGCGCCAG CCCATGATCG TGGACCGGCC GGGAGTGTCG 
CGCGAACCGT GGCACTGCCA GGGCAATGTC ACTTACGACA AGGTTGAGTT TAGCTATCCC 
ACGCGCAGAG AGATCCAGGT GCTCAAGGGT CTGGAACTGG GAGTGAAAAA GGGCCAGAAG 
GTGGCCCTGG TGGGTCCCTC CGGGTGCGGC AAGTCCACCT GCATCCAGCT GATCCAGCGC 
TTCTATGACG TGGACGAGGG TGCCGCCCTC ATCGATGAGC AGGATGTCCG AGACGTATCG 
ATGTCAAATC TGCGCCAGCA GCTCGGCATC GTGTCCCAGG AGCCGATCCT TTTTGACCGC 
ACCATCCGGC AGAACATTGC ATATGGCGAC AACACGAGGT CTGTCACCGA CCAGGAGATC 
ATGACAGCCT GCATGAAGTC TAATATTCAC GAGTTCATCG CGAACCTGCC GCTGGGTTAC 
GACACGAGAA TGGGTGAGAA GGGAGCCCAG CTGTCCGGCG GTCAGAAGCA GCGCATTGCC 
ATCGCGCGCG CCCTCATCCG GAACCCCAAG ATCATGCTGC TCGACGAAGC CACCTCGGCA 
CTGGACGCCG AGAGTGAAAA GGTTGTCCAA GATGCTCTGG ATGCCGCATC CGAGGGTCGC 
ACAACGATCA GCATAGCTCA CAGACTGTCC ACAGTCGTCC ATTCGGATGT GATCTTTGTG 
TTTGAGAACG GCGTAGTCTG CGAGACCGGC TCCCACAAGG ATTTGCTGGA GAATCGCGGC 
CTCTACTACA CGCTCTACAA GCTGCAGAGC GGGGCCATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA21135 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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